研究者詳細 - 秋山　謙太郎

ハイドロキシアパタイトを援用した骨再生におけるCCN2/CTGFの効果

大島正充, 大野充昭, 久保田聡, 藤澤拓生, 園山亘, 秋山謙太郎, 川木晴美, 西田崇, 滝川正春, 窪木拓男

日本骨代謝学会学術集会プログラム抄録集 25回 254 - 254 2007年6月

Promotion of attachment of human bone marrow stromal cells by CCN2 査読

Mitsuaki Ono, Satoshi Kubota, Takuo Fujisawa, Wataru Sonoyama, Harumi Kawaki, Kentaro Akiyama, Masamitsu Oshima, Takashi Nishida, Yasuhlro Yoshida, Kazuomi Suzuki, Masaharu Takigawa, Takuo Kuboki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 357 ( 1 ) 20 - 25 2007年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE

Cell attachment is a crucial step in tissue regeneration. In this study, human bone marrow stromal cells (hBMSCs) were isolated, and the effects of CCN2 on their attachment were examined. CCN2 significantly enhanced the hBMSC attachment, and this enhanced cell attachment was mainly regulated by the C-terminal module of CCN2. This enhancement was negated by the anti-integrin alpha(v)beta(3) antibody and p38 MAPK inhibitor, and phosphorylation of p38 MAPK was detected upon the enhanced cell attachment mediated by CCN2. We thus conclude that CCN2 enhances hBMSC attachment via integrin-p38 MAPK signal pathway. Enhanced hBMSC attachment on hydroxyapatite plates by CCN2 further indicated the utility of CCN2 in bone regeneration. (c) 2007 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2007.03.052

骨芽細胞様細胞(MC3T3E1)分化に対するチタンの影響

秋山謙太郎, 藤澤拓生, 明貝文夫, 大野充明, 吉田靖弘, 高柴正悟, 鈴木一臣, 窪木拓男

日本骨代謝学会学術集会プログラム抄録集 24回 229 - 229 2006年7月

結合組織成長因子CCN2/CTGFによる骨髄由来間質細胞の細胞接着、遊走の亢進とシグナル伝達経路の活性化

大野充昭, 藤澤拓生, 久保田聡, 園山亘, 秋山謙太郎, 西田崇, 滝川正春, 窪木拓男

日本骨代謝学会学術集会プログラム抄録集 24回 248 - 248 2006年7月

骨芽細胞様細胞株(MC3T3E1細胞)の細胞接着,増殖,分化および遺伝子発現に対するチタンの影響

秋山謙太郎, 藤澤拓生, 明貝文夫, 完山学, 吉田靖弘, 高柴正悟, 鈴木一臣, 窪木拓男

日本口腔インプラント学会誌 19 ( 1 ) 32 - 32 2006年3月

結合組織成長因子CCN2/CTGF/Hcs24はヒト骨髄由来間質細胞の細胞接着を促進させる

大野充昭, 園山亘, 藤澤拓生, 秋山謙太郎, 前川賢治, 完山学, 西田崇, 久保田聡, 滝川正春, 窪木拓男

日本骨代謝学会学術集会プログラム抄録集 23回 225 - 225 2005年6月

ヒト歯髄細胞の細胞接着,増殖,分化に与える各種成長因子ならびにアパタイトの影響

園山亘, 大野充昭, 秋山謙太郎, 藤沢拓生, 窪木拓男

日本補綴歯科学会雑誌 48 ( 111回特別 ) 211 - 211 2004年5月

Connective tissue growth factor expressed in rat alveolar bone regeneration sites after tooth extraction 査読

M Kanyama, T Kuboki, K Akiyama, K Nawachi, FM Miyauchi, H Yatani, S Kubota, T Nakanishi, M Takigawa

ARCHIVES OF ORAL BIOLOGY 48 ( 10 ) 723 - 730 2003年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD

Objective: To understand bone regeneration process after tooth extraction could be a clue to develop a new strategy for alveolar bone reconstruction. Recently, accumulated evidences support that connective tissue growth factor (CTGF) is implicated in tissue repair of many tissues. In this study, we investigated the spatial and temporal expression of CTGF in the rat tooth extraction sockets. Design: Five weeks old wild type mate rats (weighing 120 g) were used for this experiment. Expression of CTGF was determined by immunohistochemistry and in situ hybridization in the rat upper molar tooth extraction sockets at 2, 4, 7, 10 and 14 days after tooth extraction. Results: CTGF was expressed strongly in the endothelial. cells migrating into the granulation tissue at the bottom of the sockets during 4 days after tooth extraction. During the reparative process, no apparent chondrocyte-like cell appeared in the sockets, while osteoblast-like cells proliferated in the sockets with low CTGF expression at 7, 10, 14 days after extraction. As expected, no staining was observed with the preimmune rabbit IgG and CTGF sense probe. CTGF may play an important rote in angiogenesis and granulation tissue formation specifically at early heating stage after tooth extraction to initiate alveolar bone repair. Conclusion: CTGF was expressed at early heating stage of the rat tooth extraction wound. (C) 2003 Elsevier Ltd. All. rights reserved.

DOI： 10.1016/S0003-9969(03)00153-5

Cbfa1/Runx2 gene expression in articular chondrocytes of the mice temporomandibular and knee joints in vivo 査読

T Kuboki, M Kanyama, T Nakanishi, K Akiyama, K Nawachi, H Yatani, K Yamashita, T Takano-Yamamoto, M Takigawa

ARCHIVES OF ORAL BIOLOGY 48 ( 7 ) 519 - 525 2003年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD

Healthy articular cartilage is thought to be maintained by the modulation of Cbfa1 expression, although little is currently known about Cbfa1 expression in such tissues. Therefore, we examined in vivo Cbfa1 transcript levels in the temporomandibular (TM) and knee joints of 3- and 10-week-old mate ICR mice (weighing 50-70 g). A digoxigenin-11-UTP-labeled single-stranded RNA probe (0.6 kbp PstI-HindIII fragment of the 3' of untranslated region in exon 8 of mouse Cbfa1 cDNA) was prepared and in situ hybridization was performed on paraffin-embedded TM and knee joint sections. The antisense probe detected Cbfa1 transcripts in prehypertrophic chondrocytes, but not in the articular surface layer chondrocytes, of 3- and 10-week-old mice TMJs. Despite the intense Cbfa1 expression in prehypertrophic chondrocytes, articular surface layer chondrocytes of the knee joints expressed tow and undetectable level of Cbfa1 in the 3- and 10-week-old mice, respectively. These results indicate that Cbfa1 are highly expressed in the prehypertrophic chondrocytes presumably for articular tissue remodeling during the entire lifespan of the mouse, whereas Cbfa1 expression is suppressed in the articular surface chondrocytes in the adult mouse TM and knee joints to obtain the permanent cartilage phenotype. (C) 2003 Elsevier Science Ltd. All rights reserved.

DOI： 10.1016/S0003-9969(03)00088-8