研究者詳細 - 鶴田　剛司

論文 - 鶴田　剛司

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Specific Heat-Killed Lactic Acid Bacteria Enhance Mucosal Aminopeptidase N Activity in the Small Intestine of Aged Mice. 査読国際誌

Takeshi Tsuruta, Mami Wakisaka, Takumi Watanabe, Aoi Nishijima, Akihito Ikeda, Mao Teraoka, Tianyang Wang, Kuiyi Chen, Naoki Nishino

International journal of molecular sciences 26 ( 12 ) 2025年6月

記述言語：英語掲載種別：研究論文（学術雑誌）

Aminopeptidase N (APN), an enzyme expressed in the small intestinal mucosa, is involved in dietary protein digestion. Previous studies have shown that oral administration of fermented milk containing lactic acid bacteria (LAB) enhances mucosal APN activity in young mice. This study aimed to investigate whether LAB strains stimulate mucosal APN activity in aged mice and to evaluate its relevance to age-related changes in body composition. The underlying molecular mechanisms were also explored in vitro. Experiment 1: Aged C57BL/6J mice were fed diets supplemented with heat-killed LAB strains-Enterococcus faecalis OU-23 (EF), Leuconostoc mesenteroides OU-03 (LM), or Lactiplantibacillus plantarum SNK12 (LP). Compared to the aged Control group, the ileal APN activity was significantly higher in the LP group. LP administration also elevated serum Gla-osteocalcin levels and decreased serum CTX-1 levels. Experiment 2: IEC-6 cells were co-cultured with LP that had been treated with RNase, DNase, or lysozyme. APN activity was significantly lower in cells co-cultured with DNase- or lysozyme-treated LP compared to those co-cultured with untreated LP. A specific LAB strain may enhance mucosal APN activity in the aged intestine, potentially contributing to improved bone metabolism. This effect may be mediated by bacterial DNA and peptidoglycan.

DOI： 10.3390/ijms26125742

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Extracellular vesicles shed by macrophages co-cultured with <i>Lactiplantibacillus plantarum</i> No. 14 reduce adipocyte size in mice fed a high-fat diet 査読

Takeshi TSURUTA, Yohei ITO, Kei SONOYAMA

Bioscience of Microbiota, Food and Health 2025年

Cyclic Oligosaccharide-Induced Modulation of Immunoglobulin A Reactivity to Gut Bacteria Contributes to Alterations in the Bacterial Community Structure 査読

Taisei Miyamoto, Takeshi Tsuruta, Mao Teraoka, Tianyang Wang, Naoki Nishino

Nutrients 16 ( 17 ) 2824 - 2824 2024年8月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Immunoglobulin A (IgA) is a major gut antibody that coats commensal gut bacteria and contributes to shaping a stable gut bacterial composition. Although previous studies have shown that cyclic oligosaccharides, including cyclic nigerosyl-1,6-nigerose (CNN) and cyclodextrins (CDs, including αCD, βCD, and γCD), alter the gut bacterial composition, it remains unclear whether cyclic oligosaccharides modify the IgA coating of gut bacteria, which relates to cyclic oligosaccharide-induced alteration of the gut bacterial composition. To address this issue, mice were maintained for 12 weeks on diets containing CNN, αCD, βCD, or γCD; the animals’ feces were evaluated for their bacterial composition and the IgA coating index (ICI), a measure of the degree of IgA coating of bacteria. We observed that the intake of each cyclic oligosaccharide altered the gut bacterial composition, with changes in the ICI found at both the phylum and genus levels. The ICI for Bacillota, Lachnospiraceae NK4A136 group, UC Lachnospiraceae, and Tuzzerella were significantly and positively correlated with the relative abundance (RA) in total bacteria for these bacteria; in contrast, significant correlations were not seen for other phyla and genera. Our observations suggest that cyclic oligosaccharide-induced modulation of the IgA coating of gut bacteria may partly relate to changes in the community structure of the gut bacteria.

DOI： 10.3390/nu16172824

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Unabsorbed Fecal Fat Content Correlates with a Reduction of Immunoglobulin a Coating of Gut Bacteria in High-Lard Diet-Fed Mice. 査読国際誌

Emiko Katsumata, Takeshi Tsuruta, Kei Sonoyama, Takashi Yoshida, Mio Sasaki, Mao Teraoka, Tianyang Wang, Naoki Nishino

Molecular nutrition & food research 68 ( 15 ) e2400078 2024年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

SCOPE: Immunoglobulin A (IgA) selectively coats gut bacteria and contributes to regulatory functions in gastrointestinal inflammation and glucose metabolism. Excess intake of lard leads to decrease in the IgA coating of gut bacteria, although the underlying mechanisms remain unknown. This study validates how unabsorbed fat derived from a high-lard diet in the gut affects the IgA coating of bacteria, as assessed in mouse models using three types of dietary fat (lard, medium-, and long-chain triglycerides [MLCTs], and medium-chain triglycerides [MCTs]) exhibiting different digestibilities. METHODS AND RESULTS: C57BL/6J mice are maintained on diets containing lard, MLCTs, or MCTs at 7% or 30% w/w for 10 weeks (n = 6 per group). The fecal fatty acid concentration is measured to quantify unabsorbed fat content. The ratio of IgA-coated bacteria to total bacteria (IgA coating ratio) in the feces is measured by flow cytometry. Compared to lard-fed mice, MLCT- and MCT-fed mice exhibit lower fecal concentrations of palmitic acid, stearic acid, and oleic acid and higher IgA coating ratios at both 7% and 30% dietary fat, and these parameters exhibit significant negative correlations. CONCLUSION: Unabsorbed fat content in the gut may result in attenuated IgA coating of bacteria in high-lard diet-fed mice.

DOI： 10.1002/mnfr.202400078

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Cyclic Nigerosylnigerose Attenuates High-Fat Diet-Induced Fat Deposition, Colonic Inflammation, and Abnormal Glucose Metabolism and Modifies Gut Immunoglobulin a Reactivity to Commensal Bacteria. 査読国際誌

Takeshi Tsuruta, Kei Sonoyama, Taisei Miyamoto, Qui D Nguyen, Akiko Mizote, Mao Teraoka, Naoki Nishino

Molecular nutrition & food research 67 ( 14 ) e2200389 2023年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

SCOPE: High-fat diet (HFD) intake induces gut dysbiosis, inflammation in the peripheral tissues, and a reduction in immunoglobulin A (IgA) coating of gut bacteria, which is related to HFD-induced insulin resistance (IR). This study evaluates the effect of cyclic nigerosylnigerose (CNN), a dietary fiber that prevents gut inflammation and promotes IgA coating of gut bacteria, on the above-mentioned HFD-induced disorders. METHODS AND RESULTS: Balb/c mice are fed an HFD and administered CNN for 20 weeks. CNN administration reduces mesenteric adipose tissue weight, colonic tumor necrosis factor α (TNFα) mRNA expression, and serum endotoxin levels and ameliorates HFD-induced abnormal glucose metabolism. Additionally, CNN administration promotes gut bacteria-specific IgA secretion and alters IgA reactivity to gut bacteria. The alterations of IgA reactivity to specific bacteria such as Erysipelatoclostridium, Escherichia, Faecalibaculum, Lachnospiraceae genera, and Stenotrophomonas are correlated with mesenteric adipose tissue weight, colonic TNFα mRNA expression, serum endotoxin levels, and a homeostasis model assessment for IR. CONCLUSION: CNN-induced alterations in IgA reactivity to gut bacteria may be related to the suppression of HFD-induced fat deposition, colonic inflammation, endotoxemia, and IR. These observations indicate that dietary fiber that modulates IgA reactivity to gut bacteria may be useful in preventing HFD-induced disorders.

DOI： 10.1002/mnfr.202200389

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Modulatory Effects of A1 Milk, A2 Milk, Soy, and Egg Proteins on Gut Microbiota and Fermentation. 査読国際誌

Nuomin, Riyang Baek, Takeshi Tsuruta, Naoki Nishino

Microorganisms 11 ( 5 ) 2023年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

Milk can be divided into A1 and A2 types according to β-casein variants, and there is a debate about whether A1 milk consumption exacerbates gut environments. This study examined the cecum microbiota and fermentation in mice fed A1 casein, A2 casein, mixed casein (commercial casein), soy protein isolate, and egg white. The cecum acetic acid concentration was higher, and the relative abundances of Muribaculaceae and Desulfovibrionaceae were greater in mice fed A1 versus A2 casein. The other parameters of cecum fermentation and microbiota composition were similar among the mice fed A1, A2, and mixed caseins. The differences were more distinctive among the three caseins, soy, and egg feedings. Chao 1 and Shannon indices of the cecum microbiota were lowered in egg white-fed mice, and the microbiota of mice fed milk, soy, and egg proteins were separately grouped by principal coordinate analysis. Mice fed the three caseins were characterized by a high abundance of Lactobacillaceae and Clostridiaceae, those fed soy were characterized by Corynebacteriaceae, Muribaculaceae, and Ruminococcaceae, and those fed egg white were characterized by Eggerthellaceae, Rikenellaceae, and Erysipelatoclostridiaceae. Thus, although several differences can arise between A1 and A2 caseins in terms of their modulatory effects on gut environments, the differences between milk, soy, and egg proteins can be more distinctive and are worth further consideration.

DOI： 10.3390/microorganisms11051194

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Bacterial and fungal microbiota of total mixed ration silage stored at various temperatures. 査読国際誌

Ajmal Wali, Jianjian Hou, Takeshi Tsuruta, Naoki Nishino

Journal of applied microbiology 133 ( 2 ) 579 - 590 2022年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

AIMS: To obtain insights into how bacterial and fungal microbiota and fermentation products composition are affected by storage temperature for TMR silage, which can be manufactured year-round. METHODS AND RESULTS: TMR silage was stored at 10°C, 25°C, ambient temperature (AT; 20-35°C) and 40°C. Lactic acid production was delayed when stored at 10°C, and acid production stagnated after 2 weeks when stored at 40°C. The patterns of acetic acid and ethanol production were inversely related, with ethanol production promoted at 10°C and 25°C and acetic acid production promoted at AT and 40°C. The bacterial diversity was reduced in TMR silage with high lactic acid and acetic acid content, and the fungal diversity was reduced in TMR silage with high ethanol content. CONCLUSIONS: The intensity of lactic acid production was accounted for by the high abundance of Lactobacillus, and its stagnated production at a substantially high storage temperature was related to an increased abundance of Bacillus. The enhanced production of acetic acid or ethanol can be explained by differences in the fungal microbiota. SIGNIFICANCE AND IMPACT OF THE STUDY: The integrated analysis of bacterial and fungal microbiota can provide in-depth insights into the impact of storage temperature on TMR silage fermentation.

DOI： 10.1111/jam.15582

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Fecal, Milk, Uterine, Airborne Dust, and Water Microbiota in Dairy Farms in Southern Vietnam: A Pilot Study 査読

Tu Thi Minh Tran, Diep Hoang Tran, Thuong Thi Nguyen, Tomas J. Acosta, Takeshi Tsuruta, Naoki Nishino, Hai Thanh Duong

Advances in Animal and Veterinary Sciences 10 ( 7 ) 1525 - 1531 2022年7月

Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissue 査読

Manami SEKI, Akiho MIWA, Fumina OHSAKA, Yugo KARATSU, Takeshi TSURUTA, Shingo HINO, Tatsuya MORITA, Kei SONOYAMA

Bioscience of Microbiota, Food and Health 41 ( 2 ) 54 - 65 2022年

Aicda deficiency exacerbates high-fat diet-induced hyperinsulinemia but not gut dysbiosis in mice 査読

Takeshi Tsuruta, Teresia Aluoch Muhomah, Kei Sonoyama, Qui D. Nguyen, Yurika Takase, Aoi Nishijima, Shiori Himoto, Emiko Katsumata, Naoki Nishino

Nutrition Research 93 15 - 26 2021年9月

Consumption of indigestible saccharides and administration of Bifidobacterium pseudolongum reduce mucosal serotonin in murine colonic mucosa 査読

Misa Tatsuoka, Yosuke Osaki, Fumina Ohsaka, Takeshi Tsuruta, Yoshihiro Kadota, Takumi Tochio, Shingo Hino, Tatsuya Morita, Kei Sonoyama

British Journal of Nutrition 1 - 13 2021年4月

掲載種別：研究論文（学術雑誌）出版者・発行元：Cambridge University Press (CUP)

<title>Abstract</title>
SCFA increase serotonin (5-hydroxytryptamine, 5-HT) synthesis and content in the colon <italic>in vitro</italic> and <italic>ex vivo</italic>, but little is known <italic>in vivo</italic>. We tested whether dietary indigestible saccharides, utilised as a substrate to produce SCFA by gut microbiota, would increase colonic 5-HT content in mice. Male C57BL/6J mice were fed a purified diet and water supplemented with 4 % (w/v) 1-kestose (KES) for 2 weeks. Colonic 5-HT content and enterochromaffin (EC) cell numbers were lower in mice supplemented with KES than those without supplementation, while monoamine oxidase A activity and mRNA levels of tryptophan hydroxylase 1 (<italic>Tph1</italic>), chromogranin A (<italic>Chga</italic>), <italic>Slc6a4</italic> and monoamine oxidase A (<italic>Maoa</italic>) genes in the colonic mucosa, serum 5-HT concentration and total 5-HT content in the colonic contents did not differ between groups. Caecal acetate concentration and <italic>Bifidobacterium pseudolongum</italic> population were higher in KES-supplemented mice. Similar trends were observed in mice supplemented with other indigestible saccharides, that is, fructo-oligosaccharides, inulin and raffinose. Intragastric administration of live <italic>B. pseudolongum</italic> (10⁸ colony-forming units/d) for 2 weeks reduced colonic 5-HT content and EC cell numbers. These results suggest that changes in synthesis, reuptake, catabolism and overflow of 5-HT in the colonic mucosa are not involved in the reduction of colonic 5-HT content by dietary indigestible saccharides in mice. We propose that gut microbes including <italic>B. pseudolongum</italic> could contribute to the reduction of 5-HT content in the colonic mucosa via diminishing EC cells.

DOI： 10.1017/s0007114521001306

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Cecum microbiota in rats fed soy, milk, meat, fish, and egg proteins with prebiotic oligosaccharides. 査読国際誌

Souliphone Sivixay, Gaowa Bai, Takeshi Tsuruta, Naoki Nishino

AIMS microbiology 7 ( 1 ) 1 - 12 2021年

記述言語：英語掲載種別：研究論文（学術雑誌）

Diet is considered the most influential factor in modulating the gut microbiota but how dietary protein sources differ in their modulatory effects is not well understood. In this study, soy, meat (mixture of beef and pork), and fish proteins (experiment 1) and soy, milk (casein), and egg proteins (experiment 2) were fed to rats with cellulose (CEL) and raffinose (RAF); the microbiota composition and short-chain fatty acid concentration in the cecum were determined. Egg protein feeding decreased the concentration of acetic acid and the richness and diversity of the cecum microbiota. RAF feeding increased the concentrations of acetic and propionic acids and decreased the richness and diversity of the cecum microbiota. When fed with CEL, the abundance of Ruminococcaceae and Christensenellaceae, Akkermansiaceae and Tannerellaceae, and Erysipelotrichaceae enhanced with soy protein, meat and fish proteins, and egg protein, respectively. The effects of dietary proteins diminished with RAF feeding and the abundance of Bifidobacteriaceae, Erysipelotrichaceae, and Lachnospiraceae increased and that of Ruminococcaceae and Christensenellaceae decreased regardless of the protein source. These results indicate that, although the effect of prebiotics is more robust and distinctive, dietary protein sources may influence the composition and metabolic activities of the gut microbiota. The stimulatory effects of soy, meat, and egg proteins on Christensenellaceae, Akkermansiaceae, and Erysipelotrichaceae deserve further examination to better elucidate the dietary manipulation of the gut microbiota.

DOI： 10.3934/microbiol.2021001

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Expression of serotonin receptor HTR4 in glucagon-like peptide-1-positive enteroendocrine cells of the murine intestine 査読

Motoshi Okumura, Akihiro Hamada, Fumina Ohsaka, Takeshi Tsuruta, Tohru Hira, Kei Sonoyama

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 472 ( 10 ) 1521 - 1532 2020年10月

Examination of milk microbiota, fecal microbiota, and blood metabolites of Jersey cows in cool and hot seasons. 査読国際誌

Qui D Nguyen, Takeshi Tsuruta, Naoki Nishino

Animal science journal = Nihon chikusan Gakkaiho 91 ( 1 ) e13441 2020年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Microbiota of individual cow milk, bulk tank milk, and feces of Jersey cows were examined. Samples were collected from two farms (F1 and F2) in cool (November, Nov) and hot (July, Jul) seasons. Milk yield and milk composition were similar between the two farms and between the two seasons. Prevalent taxa of the fecal microbiota, i.e. Ruminococcaceae, Bacteroidaceae, Lachnospiraceae, Rikenellaceae, and Clostridiaceae, were unaffected by the farm and season. Relative abundance of milk microbiota for Pseudomonadaceae, Enterobacteriaceae, and Streptococcaceae (F1 > F2) and Lactobacillaceae, Bifidobacteriaceae, and Cellulomonadaceae (F1 < F2) were different between the two farms, and those for Staphylococcaceae, Bacillaceae, Ruminococcaceae, and Veillonellaceae (Nov < Jul) and Methylobacteriaceae and Moraxellaceae (Nov > Jul) were different between the two seasons. The microbiota of bulk tank milk was numerically different from that of individual cow milk. Principal coordinate analysis indicated that the milk microbiota was unrelated to the fecal microbiota. The finding that relative abundance of Pseudomonadaceae and Moraxellaceae appeared greater than those reported for Holstein milk suggested that higher protein and fat content may result in a greater abundance of proteolytic and lipolytic taxa in Jersey cow milk.

DOI： 10.1111/asj.13441

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Cyclic nigerosylnigerose ameliorates DSS-induced colitis with restoration of goblet cell number and increase in IgA reactivity against gut microbiota in mice. 査読

Takeshi Tsuruta, Emiko Katsumata, Akiko Mizote, Hou Jian Jian, Teresia Aluoch Muhomah, Naoki Nishino

Bioscience of microbiota, food and health 39 ( 3 ) 188 - 196 2020年

記述言語：英語掲載種別：研究論文（学術雑誌）

Cyclic nigerosylnigerose (CNN) is a cyclic oligosaccharide. Oral administration of CNN promotes immunoglobulin A (IgA) secretion in the gut. IgA is a major antibody secreted into the gut and plays a crucial role in suppressing gut inflammation due to commensal gut microbiota. To investigate the effect of administration of CNN to promote IgA secretion on gut inflammation, experimental colitis was induced with dextran sulfate sodium (DSS) in Balb/c mice after 6 weeks of CNN pre-feeding. The severity of colitis was evaluated based on a disease activity index (DAI), the gene expression of inflammatory cytokines, and a histological examination. The CNN-treated mice with DSS-induced colitis (CNN-DSS group) showed significantly lower DAI scores and mRNA levels of interleukin-1 compared with the CNN-untreated mice with DSS-induced colitis (DSS group). Histological examination of the colon revealed that the pathological score was significantly lower in the CNN-DSS group compared with the DSS group due to the reduced infiltration of immune cells. The number of goblet cells was significantly higher in the CNN-DSS group compared with the DSS group. The IgA concentration and the ratio of microbiota coated with IgA were evaluated in the cecal content. Although there was no difference in the IgA concentration among groups, a higher proportion of cecal microbiota were coated with IgA in the CNN-DSS group compared with that in the DSS group. These results suggest that CNN might preserve goblet cells in the colon and promote IgA coating of gut microbiota, which synergistically ameliorate gut inflammation in mice with DSS-induced colitis.

DOI： 10.12938/bmfh.2020-012

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The Relationship between Uterine, Fecal, Bedding, and Airborne Dust Microbiota from Dairy Cows and Their Environment: A Pilot Study. 査読

Nguyen, T. T, Miyake, A, Tran, T, Tsuruta, T, Nishino, N

9 ( 12 ) 1007 2019年

Rumen fluid, feces, milk, water, feed, airborne dust, and bedding microbiota in dairy farms managed by automatic milking systems. 査読

Wu, H, Nguyen, Q. D, Tran, T. T, Tang, M. T, Tsuruta, T, Nishino, N

Animal Science Journal 90 ( 3 ) 445 - 452 2019年

High-fat diet reduces the level of secretory immunoglobulin A coating of commensal gut microbiota. 査読

Muhomah, T. A, Nishino, N, Katsumata, E, Haoming, W, Tsuruta, T

Bioscience of microbiota, food and health 18 27 2019年

Dietary soy, meat, and fish proteins modulate the effects of prebiotic raffinose on composition and fermentation of gut microbiota in rats 査読

Gaowa Bai, Takeshi Tsuruta, Naoki Nishino

International Journal of Food Sciences and Nutrition 69 ( 4 ) 480 - 487 2018年5月

Extra-adrenal glucocorticoids contribute to the postprandial increase of circulating leptin in mice 査読

Tomabechi Y, Tsuruta T, Saito S, Wabitsch M, Sonoyama K

12 ( 2 ) 433 - 439 2018年

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