2022/02/02 更新

写真a

オガワ ヒロヒト
小川 寛人
OGAWA Hirohito
所属
医歯薬学域 助教
職名
助教
外部リンク

学位

  • 博士(獣医学) ( 岐阜大学 )

研究キーワード

  • Zoonosis

  • 人獣共通感染症

  • 病原微生物学

  • Virology

  • Microbiology

  • アフリカ

  • ヘルペスウイルス

  • 疫学

  • アデノウイルス

  • フィロウイルス

  • ウイルス学

研究分野

  • ライフサイエンス / 胎児医学、小児成育学

  • ライフサイエンス / ウイルス学

学歴

  • 岐阜大学   The United Graduate School of Veterinary Sciences  

    2004年4月 - 2007年3月

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    国名: 日本国

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経歴

  • 岡山大学   大学院医歯薬学総合研究科   助教

    2015年4月 - 現在

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  • The University of Zambia   講師

    2010年5月 - 2018年5月

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  • 北海道大学   人獣共通感染症リサーチセンター   特任助教

    2010年3月 - 2015年3月

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  • 一般財団法人日本生物科学研究所   研究員

    2007年4月 - 2010年2月

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所属学協会

 

論文

  • Nectin-2 Acts as a Viral Entry Mediated Molecule That Binds to Human Herpesvirus 6B Glycoprotein B 査読

    Hirohito Ogawa, Daisuke Fujikura, Hikaru Namba, Nobuko Yamashita, Tomoyuki Honda, Masao Yamada

    Viruses   14 ( 1 )   160 - 160   2022年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    Human herpesvirus 6B (HHV-6B) is a T-lymphotropic virus and the etiological agent of exanthem subitum. HHV-6B is present in a latent or persistent form after primary infection and is produced in the salivary glands or transmitted to this organ. Infected individuals continue to secrete the virus in their saliva, which is thus considered a source for virus transmission. HHV-6B primarily propagates in T cells because its entry receptor, CD134, is mainly expressed by activated T cells. The virus then spreads to the host’s organs, including the salivary glands, nervous system, and liver. However, CD134 expression is not detected in these organs. Therefore, HHV-6B may be entering cells via a currently unidentified cell surface molecule, but the mechanisms for this have not yet been investigated. In this study, we investigated a CD134-independent virus entry mechanism in the parotid-derived cell line HSY. First, we confirmed viral infection in CD134-membrane unanchored HSY cells. We then determined that nectin cell adhesion molecule 2 (nectin-2) mediated virus entry and that HHV-6B-insensitive T-cells transduced with nectin-2 were transformed into virus-permissive cells. We also found that virus entry was significantly reduced in nectin-2 knockout parotid-derived cells. Furthermore, we showed that HHV-6B glycoprotein B (gB) interacted with the nectin-2 V-set domain. The results suggest that nectin-2 acts as an HHV-6B entry-mediated protein.

    DOI: 10.3390/v14010160

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  • Serological and molecular epidemiological study on swine influenza in Zambia. 査読 国際誌

    Hayato Harima, Kosuke Okuya, Masahiro Kajihara, Hirohito Ogawa, Edgar Simulundu, Eugene Bwalya, Yongjin Qiu, Akina Mori-Kajihara, Musso Munyeme, Yoshihiro Sakoda, Takehiko Saito, Bernard M Hang'ombe, Hirofumi Sawa, Aaron S Mweene, Ayato Takada

    Transboundary and emerging diseases   2021年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Influenza A viruses (IAVs) cause highly contagious respiratory diseases in humans and animals. In 2009, a swine-origin pandemic H1N1 IAV, designated A(H1N1)pdm09 virus, spread worldwide, and has since frequently been introduced into pig populations. Since novel reassortant IAVs with pandemic potential may emerge in pigs, surveillance for IAV in pigs is therefore necessary not only for the pig industry but also for public health. However, epidemiological information on IAV infection of pigs in Africa remains sparse. In this study, we collected 246 serum and 605 nasal swab samples from pigs in Zambia during the years 2011-2018. Serological analyses revealed that 49% and 32% of the sera collected in 2011 were positive for hemagglutination-inhibition (HI) and neutralizing antibodies against A(H1N1)pdm09 virus, respectively, whereas less than 5.3% of sera collected during the following period (2012-2018) were positive in both serological tests. The positive rate and the neutralization titers to A(H1N1)pdm09 virus were higher than those to classical swine H1N1 and H1N2 IAVs. On the other hand, the positive rate for swine H3N2 IAV was very low in the pig population in Zambia in 2011-2018 (5.3% and 0% in HI and neutralization tests, respectively). From nasal swab samples, we isolated one H3N2 and eight H1N1 IAV strains with an isolation rate of 1.5%. Phylogenetic analyses of all eight gene segments revealed that the isolated IAVs were closely related to human IAV strains belonging to A(H1N1)pdm09 and seasonal H3N2 lineages. Our findings indicate that reverse zoonotic transmission from humans to pigs occurred during the study period in Zambia and highlight the need for continued surveillance to monitor the status of IAVs circulating in swine populations in Africa. This article is protected by copyright. All rights reserved.

    DOI: 10.1111/tbed.14373

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  • Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus. 査読 国際共著 国際誌

    Yoshihiro Takadate, Rashid Manzoor, Takeshi Saito, Yurie Kida, Junki Maruyama, Tatsunari Kondoh, Hiroko Miyamoto, Hirohito Ogawa, Masahiro Kajihara, Manabu Igarashi, Ayato Takada

    Microorganisms   8 ( 10 )   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lloviu virus (LLOV), a bat-derived filovirus that is phylogenetically distinct from human pathogenic filoviruses such as Ebola virus (EBOV) and Marburg virus (MARV), was discovered in Europe. However, since infectious LLOV has never been isolated, the biological properties of this virus remain poorly understood. We found that vesicular stomatitis virus (VSV) pseudotyped with the glycoprotein (GP) of LLOV (VSV-LLOV) showed higher infectivity in one bat (Miniopterus sp.)-derived cell line than in the other bat-derived cell lines tested, which was distinct from the tropism of VSV pseudotyped with EBOV (VSV-EBOV) and MARV GPs. We then focused on the interaction between GP and Niemann-Pick C1 (NPC1) protein, one of the cellular receptors of filoviruses. We introduced the Miniopterus bat and human NPC1 genes into NPC1-knockout Vero E6 cells and their susceptibilities to the viruses were compared. The cell line expressing the bat NPC1 showed higher susceptibility to VSV-LLOV than that expressing human NPC1, whereas the opposite preference was seen for VSV-EBOV. Using a site-directed mutagenesis approach, amino acid residues involved in the differential tropism were identified in the NPC1 and GP molecules. Our results suggest that the interaction between GP and NPC1 is an important factor in the tropism of LLOV to a particular bat species.

    DOI: 10.3390/microorganisms8101530

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  • Bacterial and protozoan pathogens/symbionts in ticks infecting wild grasscutters (Thryonomys swinderianus) in Ghana. 査読 国際共著 国際誌

    Christopher Adenyo, Kenji Ohya, Yongjin Qiu, Yasuhiro Takashima, Hirohito Ogawa, Tateki Matsumoto, May June Thu, Kozue Sato, Hiroki Kawabata, Yukie Katayama, Tsutomu Omatsu, Tetsuya Mizutani, Hideto Fukushi, Ken Katakura, Narikaki Nonaka, Miho Inoue-Murayama, Boniface Kayang, Ryo Nakao

    Acta tropica   205   105388 - 105388   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Ticks and tick-borne pathogens constitute a great threat to livestock production and are a potential health hazard to humans. Grasscutters (Thryonomys swinderianus) are widely hunted for meat in Ghana and many other West and Central African countries. However, tick-borne zoonotic risks posed by wild grasscutters have not been assessed. The objective of this study was to investigate bacterial and protozoan pathogens in ticks infecting wild grasscutters. A total of 81 ticks were collected from three hunted grasscutters purchased from Kantamanto, the central bushmeat market in Accra. Ticks were identified as Ixodes aulacodi and Rhipicephalus sp. based on morphological keys, which were further confirmed by sequencing mitochondrial 16S ribosomal DNA (rDNA) and cytochrome oxidase I (COI) genes of specimens. Protozoan infections were tested by PCR amplifying 18S rDNA of Babesia/Theileria/Hepatozoon, while bacterial infections were evaluated by PCRs or real-time PCRs targeting Anaplasmataceae, Borrelia, spotted fever group rickettsiae, chlamydiae and Candidatus Midichloria mitochondrii. The results of PCR screening showed that 35.5% (27 out of 76) of I. aulacodi were positive for parasite infections. Sequencing analysis of the amplified products gave one identical sequence showing similarity with Babesia spp. reported from Africa. The Ca. M. mitochondrii endosymbiont was present in 85.5% (65 out of 76) of I. aulacodi but not in the five Rhipicephalus ticks. Two Anaplasmataceae bacteria genetically related to Ehrlichia muris and Anaplasma phagocytophilum were also detected in two I. aulacodi. None of the ticks were positive for Borrelia spp., spotted fever group rickettsiae and chlamydiae. Since I. aulacodi on wild grasscutters are potential carriers of tick-borne pathogens, some of which could be of zoonotic potential, rigorous tick control and pathogen analyses should be instituted especially when wild caught grasscutters are being used as foundation stock for breeding.

    DOI: 10.1016/j.actatropica.2020.105388

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  • Comparative Analysis of Fecal Microbiota in Grasscutter (Thryonomys swinderianus) and Other Herbivorous Livestock in Ghana. 査読 国際共著 国際誌

    Kiyonori Kawasaki, Kenji Ohya, Tsutomu Omatsu, Yukie Katayama, Yasuhiro Takashima, Tsuyoshi Kinoshita, Justice Opare Odoi, Kotaro Sawai, Hideto Fukushi, Hirohito Ogawa, Miho Inoue-Murayama, Tetsuya Mizutani, Christopher Adenyo, Yoshiki Matsumoto, Boniface Kayang

    Microorganisms   8 ( 2 )   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The grasscutter (also known as the greater cane rat; Thryonomys swinderianus) is a large rodent native to West Africa that is currently under domestication process for meat production. However, little is known about the physiology of this species. In the present study, aiming to provide information about gut microbiota of the grasscutter and better understand its physiology, we investigated the intestinal microbiota of grasscutters and compared it with that of other livestock (cattle, goat, rabbit, and sheep) using 16S rRNA metagenomics analysis. Similar to the other herbivorous animals, bacteria classified as Bacteroidales, Clostridiales, Ruminococcaceae, and Lachnospiraceae were abundant in the microbiome of grasscutters. However, Prevotella and Treponema bacteria, which have fiber fermentation ability, were especially abundant in grasscutters, where the relative abundance of these genera was higher than that in the other animals. The presence of these genera might confer grasscutters the ability to easily breakdown dietary fibers. Diets for grasscutters should be made from ingredients not consumed by humans to avoid competition for resources and the ability to digest fibers may allow the use of fiber-rich feed materials not used by humans. Our findings serve as reference and support future studies on changes in the gut microbiota of the grasscutter as domestication progresses in order to establish appropriate feeding methods and captivity conditions.

    DOI: 10.3390/microorganisms8020265

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  • Detection of novel orthoreovirus genomes in shrew (Crocidura hirta) and fruit bat (Rousettus aegyptiacus). 査読

    Hayato Harima, Michihito Sasaki, Masahiro Kajihara, Akina Mori-Kajihara, Bernard M Hang'ombe, Katendi Changula, Yasuko Orba, Hirohito Ogawa, Martin Simuunza, Reiko Yoshida, Aaron Mweene, Ayato Takada, Hirofumi Sawa

    The Journal of veterinary medical science   82 ( 2 )   162 - 167   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Orthoreoviruses have been indentified in several mammals, however, there is no information about orthoreoviruses in shrews. In this study, we screened wild animals in Zambia, including shrews, rodents, and bats for the detection of orthoreoviruses. Two orthoreovirus RNA genomes were detected from a shrew intestinal-contents (1/24) and a bat colon (1/96) sample by reverse-transcription (RT)-PCR targeting the RNA-dependent RNA polymerase gene of orthoreoviruses. Phylogenetic analyses revealed that each of the identified orthoreoviruses formed a distinct branch among members of the Orthoreovirus genus. This is the first report that shrews are susceptible to orthoreovirus infection. Our results suggest the existence of undiscovered orthoreoviruses in shrews and provide important information about the genetic diversity of orthoreoviruses.

    DOI: 10.1292/jvms.19-0424

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  • Niemann-Pick C1 Heterogeneity of Bat Cells Controls Filovirus Tropism. 査読 国際共著 国際誌

    Yoshihiro Takadate, Tatsunari Kondoh, Manabu Igarashi, Junki Maruyama, Rashid Manzoor, Hirohito Ogawa, Masahiro Kajihara, Wakako Furuyama, Masahiro Sato, Hiroko Miyamoto, Reiko Yoshida, Terence E Hill, Alexander N Freiberg, Heinz Feldmann, Andrea Marzi, Ayato Takada

    Cell reports   30 ( 2 )   308 - 319   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Fruit bats are suspected to be natural hosts of filoviruses, including Ebola virus (EBOV) and Marburg virus (MARV). Interestingly, however, previous studies suggest that these viruses have different tropisms depending on the bat species. Here, we show a molecular basis underlying the host-range restriction of filoviruses. We find that bat-derived cell lines FBKT1 and ZFBK13-76E show preferential susceptibility to EBOV and MARV, respectively, whereas the other bat cell lines tested are similarly infected with both viruses. In FBKT1 and ZFBK13-76E, unique amino acid (aa) sequences are found in the Niemann-Pick C1 (NPC1) protein, one of the cellular receptors interacting with the filovirus glycoprotein (GP). These aa residues, as well as a few aa differences between EBOV and MARV GPs, are crucial for the differential susceptibility to filoviruses. Taken together, our findings indicate that the heterogeneity of bat NPC1 orthologs is an important factor controlling filovirus species-specific host tropism.

    DOI: 10.1016/j.celrep.2019.12.042

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  • Marburgvirus in Egyptian Fruit Bats, Zambia. 査読 国際誌

    Masahiro Kajihara, Bernard M Hang'ombe, Katendi Changula, Hayato Harima, Mao Isono, Kosuke Okuya, Reiko Yoshida, Akina Mori-Kajihara, Yoshiki Eto, Yasuko Orba, Hirohito Ogawa, Yongjin Qiu, Hirofumi Sawa, Edgar Simulundu, Daniel Mwizabi, Musso Munyeme, David Squarre, Victor Mukonka, Aaron Mweene, Ayato Takada

    Emerging infectious diseases   25 ( 8 )   1577 - 1580   2019年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We detected Marburg virus genome in Egyptian fruit bats (Rousettus aegyptiacus) captured in Zambia in September 2018. The virus was closely related phylogenetically to the viruses that previously caused Marburg outbreaks in the Democratic Republic of the Congo. This finding demonstrates that Zambia is at risk for Marburg virus disease.

    DOI: 10.3201/eid2508.190268

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  • Risk assessment for hepatitis E virus infection from domestic pigs introduced into an experimental animal facility in a medical school. 査読

    Ogawa H, Hirayama H, Tanaka S, Yata N, Namba H, Yamashita N, Yonemitsu K, Maeda K, Mominoki K, Yamada M

    The Journal of veterinary medical science   81 ( 8 )   1191 - 1196   2019年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1292/jvms.19-0086

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  • Generation of bat-derived influenza viruses and their reassortants. 査読 国際共著 国際誌

    Masahiro Sato, Junki Maruyama, Tatsunari Kondoh, Naganori Nao, Hiroko Miyamoto, Yoshihiro Takadate, Wakako Furuyama, Masahiro Kajihara, Hirohito Ogawa, Rashid Manzoor, Reiko Yoshida, Manabu Igarashi, Ayato Takada

    Scientific reports   9 ( 1 )   1158 - 1158   2019年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Two novel influenza A virus-like genomes were detected in fruit bats in Central and South America. However, the biological properties of these bat-derived influenza viruses (BatIVs) are still largely unknown since infectious viral particles have never been isolated from the infected host species. In this study, a reverse genetics approach was used to generate infectious BatIV particles entirely from plasmids encoding full-length sequences in eight gene segments. We inoculated BatIV particles into various cell cultures including bat-derived cell lines and found that BatIVs infected particular bat-derived cells efficiently but not the other cell lines tested. Reassortant viruses between the two BatIVs were also successfully generated and their replication in the susceptible bat cell lines was confirmed. These findings suggest a limited host range and reassortment potential of BatIVs in nature, providing fundamental information for understanding of the ecology of BatIVs.

    DOI: 10.1038/s41598-018-37830-x

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  • Identification of group A rotaviruses from Zambian fruit bats provides evidence for long-distance dispersal events in Africa 査読

    Michihito Sasaki, Masahiro Kajihara, Katendi Changula, Akina Mori-Kajihara, Hirohito Ogawa, Bernard M. Hang'ombe, Aaron S. Mweene, Martin Simuunza, Reiko Yoshida, Michael Carr, Yasuko Orba, Ayato Takada, Hirofumi Sawa

    Infection, Genetics and Evolution   63   104 - 109   2018年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier B.V.  

    Group A rotavirus (RVA) is a major cause of diarrhea in children worldwide. Although RVA infects many animals, little is known about RVA in bats. The present study investigated the genetic diversity of RVA in Zambian bats. We identified RVA from two straw-colored fruit bats (Eidolon helvum) and an Egyptian fruit bat (Rousettus aegyptiacus), and analyzed the genome sequences of these strains. Genome segments of the RVA strains from Zambian E. helvum showed 97%–99% nucleotide sequence identity with those of other RVA strains from E. helvum in Cameroon, which is 2800 km from the sampling locations. These findings suggest that migratory straw-colored fruit bat species, distributed across sub-Saharan Africa, have the potential to disseminate RVA across long distances. By contrast, the RVA strain from Zambian R. aegyptiacus carried highly divergent NSP2 and NSP4 genes, leading us to propose novel genotypes N21 and E27, respectively. Notably, this RVA strain also shared the same genotype for VP6 and NSP3 with the RVA strains from Zambian E. helvum, suggesting interspecies transmission and genetic reassortment may have occurred between these two bat species in the past. Our study has important implications for RVA dispersal in bat populations, and expands our knowledge of the ecology, diversity and evolutionary relationships of RVA.

    DOI: 10.1016/j.meegid.2018.05.016

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  • Loss of Bacitracin Resistance Due to a Large Genomic Deletion among Bacillus anthracis Strains. 査読

    Furuta Y, Harima H, Ito E, Maruyama F, Ohnishi N, Osaki K, Ogawa H, Squarre D, Hang'ombe BM, Higashi H

    mSystems   3 ( 5 )   2018年9月

  • Co-circulation of multiple genotypes of African swine fever viruses among domestic pigs in Zambia (2013–2015) 査読

    E. Simulundu, H. M. Chambaro, Y. Sinkala, M. Kajihara, H. Ogawa, A. Mori, J. Ndebe, G. Dautu, L. Mataa, C. H. Lubaba, C. Simuntala, P. Fandamu, M. Simuunza, G. S. Pandey, K. L. Samui, G. Misinzo, A. Takada, A. S. Mweene

    Transboundary and Emerging Diseases   65 ( 1 )   114 - 122   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Blackwell Publishing Ltd  

    During 2013–2015, several and severe outbreaks of African swine fever (ASF) affected domestic pigs in six provinces of Zambia. Genetic characterization of ASF viruses (ASFVs) using standardized genotyping procedures revealed that genotypes I, II and XIV were associated with these outbreaks. Molecular and epidemiological data suggest that genotype II ASFV (Georgia 2007/1-like) detected in Northern Province of Zambia may have been introduced from neighbouring Tanzania. Also, a genotype II virus detected in Eastern Province of Zambia showed a p54 phylogenetic relationship that was inconsistent with that of p72, underscoring the genetic variability of ASFVs. While it appears genotype II viruses detected in Zambia arose from a domestic pig cycle, genotypes I and XIV possibly emerged from a sylvatic cycle. Overall, this study demonstrates the co-circulation of multiple genotypes of ASFVs, involvement of both the sylvatic and domestic pig cycle in ASF outbreaks in Zambia and possible trans-boundary spread of the disease in south-eastern Africa. Indeed, while there is need for regional or international concerted efforts in the control of ASF, understanding pig marketing practices, pig population dynamics, pig housing and rearing systems and community engagement will be important considerations when designing future prevention and control strategies of this disease in Zambia.

    DOI: 10.1111/tbed.12635

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  • Involvement of multiple Chlamydia suis genotypes in porcine conjunctivitis 査読

    R. Chahota, H. Ogawa, K. Ohya, T. Yamaguchi, K. D.E. Everett, H. Fukushi

    Transboundary and Emerging Diseases   65 ( 1 )   272 - 277   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Blackwell Publishing Ltd  

    Chlamydia suis has been detected in numerous disease conditions of pigs, particularly in eye infections. This study examined recurring conjunctivitis cases in five commercial pig farms in Japan. 40.5% of the cases were identified as Chlamydia positive using impression cytology of ocular smears and a genus-specific direct fluorescent antibody. C. suis was detected in 59.5% of the samples using PCR tests targeting 16S-23S rRNA intergenic spacer region (ISR) and ompA gene. Genetic analysis of PCR amplicons revealed nine sequence variants of 16S-23S rRNA ISR and 20 sequence variants within ompA gene. Among C. suis-positive conjunctivitis cases, 36.4% showed concurrent infection with 2–4 varied ompA sequence types and 9.1% showed multiple 16S-23S rRNA ISR sequence types of C. suis. Multiple genotypes were found circulating in four of five farms. All 20 detected strains and 25 previously reported C. suis strains were grouped into four clusters. Japanese C. suis strains were closely related to American and European strains indicating wide distribution of these genetically variant strains. This study is the first to show multiple and genetically diverse C. suis strain associations in pig conjunctivitis.

    DOI: 10.1111/tbed.12645

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  • Metabolic pathway catalyzed by Vanin-1 pantetheinase plays a suppressive role in influenza virus replication in human alveolar epithelial A549 cells 査読

    Nobuko Yamashita, Masato Yashiro, Hirohito Ogawa, Hikaru Namba, Nobuyuki Nosaka, Yousuke Fujii, Tsuneo Morishima, Hirokazu Tsukahara, Masao Yamada

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   489 ( 4 )   466 - 471   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Our previous analysis of gene expression profiles in the peripheral blood from patients with influenza A (H1N1) pdm09 pneumonia revealed elevated transcription levels of the vanin-1 (vascular non inflammatory molecule 1, VNN1) gene, which encodes an epithelial ectoenzyme with pantetheinase activity involved in recycling coenzyme A. Here, to elucidate the role of VNN1 in influenza A virus (IAV) H1N1 infection, we investigated the change of VNN1 expression in the context of IAV infection and the effects of its related substances, i.e., its direct substrate pantetheine and its two metabolites pantothenic acid and cysteamine on the replication of IAV in the human alveolar epithelial carcinoma cell line A549. The messenger RNA expression of VNN1 in A549 cells was significantly increased (by 4.9-fold) after IAV infection under an elevated concentration of pantetheine. Moreover, VNN1 mRNA levels were elevated by > 100-fold in response to pro-inflammatory cytokines, especially TNF-alpha and IL-1 beta. Pantetheine significantly reduced the IAV replication and IAV Matrix 1 (M1) mRNA levels when it was administered prior to and during infection. In addition, cysteamine treatment during IAV infection significantly reduced the viral replication and IAV M1 mRNA levels, whereas pantothenic acid did not. These findings suggest that the metabolic pathway catalyzed by VNNI pantetheinase plays a suppressive role in IAV infection in the respiratory tract, especially in severe conditions under hypercytokinemia. (C) 2017 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2017.05.172

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  • Monitoring of Human Herpesviruses-6 and-7 DNA in Saliva Samples During the Acute and Convalescent Phases of Exanthem Subitum 査読

    Yuki Miyazaki, Hikaru Namba, Sadayoshi Torigoe, Masahiro Watanabe, Nobuko Yamashita, Hirohito Ogawa, Tsuneo Morishima, Masao Yamada

    JOURNAL OF MEDICAL VIROLOGY   89 ( 4 )   696 - 702   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    The amounts of the DNAs of human herpesviruses-6 (HHV-6) and -7 (HHV-7) in saliva samples were monitored during the acute and convalescent phases of exanthem subitum (ES) to elucidate the kinetics of virus shedding after ES. A total of 247 saliva samples were collected from 17 children (5 males and 12 females: 8-31 months old at onset). The monitoring period ranged from 152 to 721 days after onset, and in 15 children it was longer than 1 year. Among the 17 cases, 16 were attributed to HHV-6B, while a single case was attributed to HHV-7. Detection rates and average amounts of HHV-6 DNA in saliva samples after ES attributed to HHV-6B were low in the acute phase, increased to the maximum in the convalescent phase at 3-7 months, and then decreased. In addition, to investigate the source of infection, saliva samples from the older siblings (age 3-9 years) and parents of ES patients and children with a history of ES were also examined. The detection rate of HHV-6 DNA in saliva samples from 3- to 9-year-old children was significantly higher than the rate in adult saliva samples. Taken together, these findings suggest that the saliva of children in the convalescent phase of ES might be a more likely source of HHV-6 infection than that of adults. (C) 2016 Wiley Periodicals, Inc.

    DOI: 10.1002/jmv.24690

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  • Molecular characterization of infectious bursal disease viruses detected in vaccinated commercial broiler flocks in Lusaka, Zambia 査読

    Kunda Ndashe, Edgar Simulundu, Bernard M. Hang'ombe, Ladslav Moonga, Hirohito Ogawa, Ayato Takada, Aaron S. Mweene

    ARCHIVES OF VIROLOGY   161 ( 3 )   513 - 519   2016年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER WIEN  

    Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive viral disease of young chickens and remains one of the economically most important diseases threatening the poultry industry worldwide. In this study, 16 and 11 nucleotide sequences of the VP2 hypervariable region (VP2-HVR) and part of VP1, respectively, of IBD virus (IBDV) detected in vaccinated broiler chickens in Lusaka in 2012 were determined. Phylogenetic analysis revealed that these Zambian IBDVs separated into three genotypes of very virulent (VV) IBDVs. Although the majority of these viruses belonged to the African VV type (VV1), which consisted of viruses from West Africa, South Africa and Zambia, one virus belonged to the East African VV type (VV2). Interestingly, a Zambian IBDV belonging to the VV3 genotype (composed of viruses from several continents) clustered with attenuated vaccine strains. Although sequence analysis of VP2-HVR showed that all detected Zambian IBDVs had conserved putative virulence marker amino acids (i.e., 222A, 242I, 256I, 294I and 299S), one virus had two unique amino acid substitutions, N280S and E300A. This study demonstrates the diversity of Zambian IBDVs and documents for the first time the possible involvement of attenuated vaccine strains in the epidemiology of IBD in Zambia. Strict biosecurity of poultry farms, monitoring of live vaccine use in the field, surveillance and characterization of IBDV in poultry and development of a vaccine from local or regional IBDV field strains are recommended for improved IBD control in Zambia.

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  • Characterization of the glycoproteins of bat-derived influenza viruses 査読

    Junki Maruyama, Naganori Nao, Hiroko Miyamoto, Ken Maeda, Hirohito Ogawa, Reiko Yoshida, Manabu Igarashi, Ayato Takada

    VIROLOGY   488   43 - 50   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Recently found bat-derived influenza viruses (BatlVs) have hemagglutinin (HA) and neuraminidase (NA) gene segments distinct from those of previously known influenza A viruses. However, pathogenicities of these BatlVs remain unknown since infectious virus strains have not been isolated yet. To gain insight into the biological properties of BatlVs, we generated vesicular stomatitis viruses (VSVs) pseudotyped with the BatIV HA and NA. We found that VSVs pseudotyped with BatIV HAs and NAs efficiently infected particular bat cell lines but not those derived from primates, and that proteolytic cleavage with a trypsinlike protease was necessary for HA-mediated virus entry. Treatment of the susceptible bat cells with some enzymes and inhibitors revealed that BatIV HAs might recognize some cellular glycoproteins as receptors rather than the sialic acids used for the other known influenza viruses. These data provide fundamental information on the mechanisms underlying the cellular entry and host restriction of BatIVs. (C) 2015 Elsevier Inc. All rights reserved.

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  • Seroepidemiological Prevalence of Multiple Species of Filoviruses in Fruit Bats (Eidolon helvum) Migrating in Africa 査読

    Hirohito Ogawa, Hiroko Miyamoto, Eri Nakayama, Reiko Yoshida, Ichiro Nakamura, Hirofumi Sawa, Akihiro Ishii, Yuka Thomas, Emiko Nakagawa, Keita Matsuno, Masahiro Kajihara, Junki Maruyama, Naganori Nao, Mieko Muramatsu, Makoto Kuroda, Edgar Simulundu, Katendi Changula, Bernard Hang'ombe, Boniface Namangala, Andrew Nambota, Jackson Katampi, Manabu Igarashi, Kimihito Ito, Heinz Feldmann, Chihiro Sugimoto, Ladslav Moonga, Aaron Mweene, Ayato Takada

    JOURNAL OF INFECTIOUS DISEASES   212   S101 - S108   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS INC  

    Fruit bats are suspected to be a natural reservoir of filoviruses, including Ebola and Marburg viruses. Using an enzyme-linked immunosorbent assay based on the viral glycoprotein antigens, we detected filovirus-specific immunoglobulin G antibodies in 71 of 748 serum samples collected from migratory fruit bats (Eidolon helvum) in Zambia during 2006-2013. Although antibodies to African filoviruses (eg, Zaire ebolavirus) were most prevalent, some serum samples showed distinct specificity for Reston ebolavirus, which that has thus far been found only in Asia. Interestingly, the transition of filovirus species causing outbreaks in Central and West Africa during 2005-2014 seemed to be synchronized with the change of the serologically dominant virus species in these bats. These data suggest the introduction of multiple species of filoviruses in the migratory bat population and point to the need for continued surveillance of filovirus infection of wild animals in sub-Saharan Africa, including hitherto nonendemic countries.

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  • Genetic and antigenic characterization of H5 and H7 influenza viruses isolated from migratory water birds in Hokkaido, Japan and Mongolia from 2010 to 2014 査読

    Takahiro Hiono, Ayako Ohkawara, Kohei Ogasawara, Masatoshi Okamatsu, Tomokazu Tamura, Duc-Huy Chu, Mizuho Suzuki, Saya Kuribayashi, Shintaro Shichinohe, Ayato Takada, Hirohito Ogawa, Reiko Yoshida, Hiroko Miyamoto, Naganori Nao, Wakako Furuyama, Junki Maruyama, Nao Eguchi, Gerelmaa Ulziibat, Bazarragchaa Enkhbold, Munkhduuren Shatar, Tserenjav Jargalsaikhan, Selenge Byambadorj, Batchuluun Damdinjav, Yoshihiro Sakoda, Hiroshi Kida

    VIRUS GENES   51 ( 1 )   57 - 68   2015年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Migratory water birds are the natural reservoir of influenza A viruses. H5 and H7 influenza viruses are isolated over the world and also circulate among poultry in Asia. In 2010, two H5N1 highly pathogenic avian influenza viruses (HPAIVs) were isolated from fecal samples of water birds on the flyway of migration from Siberia, Russia to the south in Hokkaido, Japan. H7N9 viruses are sporadically isolated from humans and circulate in poultry in China. To monitor whether these viruses have spread in the wild bird population, we conducted virological surveillance of avian influenza in migratory water birds in autumn from 2010 to 2014. A total of 8103 fecal samples from migratory water birds were collected in Japan and Mongolia, and 350 influenza viruses including 13 H5 and 19 H7 influenza viruses were isolated. A phylogenetic analysis revealed that all isolates are genetically closely related to viruses circulating among wild water birds. The results of the antigenic analysis indicated that the antigenicity of viruses in wild water birds is highly stable despite their nucleotide sequence diversity but is distinct from that of HPAIVs recently isolated in Asia. The present results suggest that HPAIVs and Chinese H7N9 viruses were not predominantly circulating in migratory water birds; however, continued monitoring of H5 and H7 influenza viruses both in domestic and wild birds is recommended for the control of avian influenza.

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  • Bacillus cereus from the environment is genetically related to the highly pathogenic B. cereus in Zambia 査読

    Hirohito Ogawa, Miyuki Ohnuma, David Squarre, Aaron Simanyengwe Mweene, Takayuki Ezaki, Daisuke Fujikura, Naomi Ohnishi, Yuka Thomas, Bernard Mudenda Hang'Ombe, Hideaki Higashi

    JOURNAL OF VETERINARY MEDICAL SCIENCE   77 ( 8 )   993 - 995   2015年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC VET SCI  

    To follow-up anthrax in Zambia since the outbreak in 2011, we have collected samples from the environment and the carcasses of anthrax-suspected animals, and have tried to isolate Bacillus anthracis. In the process of identification of B. anthracis, we collected two isolates, of which colonies were similar to B. anthracis; however, from the results of identification using the molecular-based methods, two isolates were genetically related to the highly pathogenic B. cereus, of which clinical manifestation is severe and fatal (e.g., pneumonia). In this study, we showed the existence of bacteria suspected to be highly pathogenic B. cereus in Zambia, indicating the possibility of an outbreak caused by highly pathogenic B. cereus.

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  • Molecular epidemiology of pathogenic Leptospira spp. in the straw-colored fruit bat (Eidolon helvum) migrating to Zambia from the Democratic Republic of Congo 査読

    Hirohito Ogawa, Nobuo Koizumi, Aiko Ohnuma, Alisheke Mutemwa, Bernard M. Hang'ombe, Aaron S. Mweene, Ayato Takada, Chihiro Sugimoto, Yasuhiko Suzuki, Hiroshi Kida, Hirofumi Sawa

    INFECTION GENETICS AND EVOLUTION   32   143 - 147   2015年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The role played by bats as a potential source of transmission of Leptospira spp. to humans is poorly understood, despite various pathogenic Leptospira spp. being identified in these mammals. Here, we investigated the prevalence and diversity of pathogenic Leptospira spp. that infect the straw-colored fruit bat (Eidolon helvum). We captured this bat species, which is widely distributed in Africa, in Zambia during 2008-2013. We detected the flagellin B gene (flaB) from pathogenic Leptospira spp. in kidney samples from 79 of 529 E. helvum (14.9%) bats. Phylogenetic analysis of 70 flaB fragments amplified from E. helvum samples and previously reported sequences, revealed that 12 of the fragments grouped with Leptospira borgpetersenii and Leptospira kirschneri; however, the remaining 58 flaB fragments appeared not to be associated with any reported species. Additionally, the 16S ribosomal RNA gene (rrs) amplified from 27 randomly chosen flaB-positive samples was compared with previously reported sequences, including bat-derived Leptospira spp. All 27 rrs fragments clustered into a pathogenic group. Eight fragments were located in unique branches, the other 19 fragments were closely related to Leptospira spp. detected in bats. These results show that rrs sequences in bats are genetically related to each other without regional variation, suggesting that Leptospira are evolutionarily well-adapted to bats and have uniquely evolved in the bat population. Our study indicates that pathogenic Leptospira spp. in E. helvum in Zambia have unique genotypes. (C) 2015 The Authors. Published by Elsevier B.V.

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  • A Novel Multiplex PCR Discriminates Bacillus anthracis and Its Genetically Related Strains from Other Bacillus cereus Group Species 査読

    Hirohito Ogawa, Daisuke Fujikura, Miyuki Ohnuma, Naomi Ohnishi, Bernard M. Hang'ombe, Hitomi Mimuro, Takayuki Ezaki, Aaron S. Mweene, Hideaki Higashi

    PLOS ONE   10 ( 3 )   e0122004   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Anthrax is an important zoonotic disease worldwide that is caused by Bacillus anthracis, a spore-forming pathogenic bacterium. A rapid and sensitive method to detect B. anthracis is important for anthrax risk management and control in animal cases to address public health issues. However, it has recently become difficult to identify B. anthracis by using previously reported molecular-based methods because of the emergence of B. cereus, which causes severe extra-intestinal infection, as well as the human pathogenic B. thuringiensis, both of which are genetically related to B. anthracis. The close genetic relation of chromosomal backgrounds has led to complexity of molecular-based diagnosis. In this study, we established a B. anthracis multiplex PCR that can screen for the presence of B. anthracis virulent plasmids and differentiate B. anthracis and its genetically related strains from other B. cereus group species. Six sets of primers targeting a chromosome of B. anthracis and B. anthracis-like strains, two virulent plasmids, pXO1 and pXO2, a bacterial gene, 16S rRNA gene, and a mammalian gene, actin-beta gene, were designed. The multiplex PCR detected approximately 3.0 CFU of B. anthracis DNA per PCR reaction and was sensitive to B. anthracis. The internal control primers also detected all bacterial and mammalian DNAs examined, indicating the practical applicability of this assay as it enables monitoring of appropriate amplification. The assay was also applied for detection of clinical strains genetically related to B. anthracis, which were B. cereus strains isolated from outbreaks of hospital infections in Japan, and field strains isolated in Zambia, and the assay differentiated B. anthracis and its genetically related strains from other B. cereus group strains. Taken together, the results indicate that the newly developed multiplex PCR is a sensitive and practical method for detecting B. anthracis.

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  • Pathological and molecular diagnosis of the 2013 African swine fever outbreak in Lusaka, Zambia 査読

    John Yabe, Pharaoh Hamambulu, Edgar Simulundu, Hirohito Ogawa, Masahiro Kajihara, Akina Mori-Kajihara, Katendi Changula-Chitanga, Max Mwase, Mutinta Mweemba-Muwowo, Herman Moses Chambaro, Liywalii Mataa, Bernard Hang'ombe, Bonniface Namangala, Paul Fandamu, Hirofumi Sawa, Ayato Takada, Hideaki Higashi, Aaron Simanyengwe Mweene

    TROPICAL ANIMAL HEALTH AND PRODUCTION   47 ( 2 )   459 - 463   2015年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    African swine fever (ASF) is a highly contagious and fatal hemorrhagic viral disease of domestic pigs. The disease is widespread in sub-Saharan Africa and has repeatedly been introduced into other continents. The current study describes the diagnostic investigations of a hemorrhagic disease that was reported in pigs in Lusaka (October 2013), Zambia. Necropsy, histopathology, and molecular diagnosis using polymerase chain reaction and sequence analysis confirmed the disease to be ASF. The sequences obtained showed high similarity to previously isolated ASF viruses. Consistent surveillance and rapid diagnosis of the disease is recommended to prevent future outbreaks and economic losses as there is currently no vaccine against the disease.

    DOI: 10.1007/s11250-014-0732-0

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  • Orthopoxvirus infection among wildlife in Zambia 査読

    Yasuko Orba, Michihito Sasaki, Hiroki Yamaguchi, Akihiro Ishii, Yuka Thomas, Hirohito Ogawa, Bernard M. Hang'ombe, Aaron S. Mweene, Shigeru Morikawa, Masayuki Saijo, Hirofumi Sawa

    JOURNAL OF GENERAL VIROLOGY   96 ( Pt 2 )   390 - 394   2015年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Human monkeypox is a viral zoonosis caused by monkeypox virus, an orthopoxvirus (OPXV). The majority of human monkeypox cases have been reported in moist forested regions in West and Central Africa, particularly in the Democratic Republic of the Congo (DRC). In this study we investigated zoonotic OPXV infection among wild animals in Zambia, which shares a border with DRC, to assess the geographical distribution of OPXV. We screened for OPXV antibodies in sera from non-human primates (NHPs), rodents and shrews by ELISA, and performed real-time PCR to detect OPXV DNA in spleen samples. Serological analysis indicated that 38 of 259 (14.7 %) rodents, 14 of 42 (33.3%) shrews and 4 of 188 (2.1 %) NHPs had antibodies against OPXV. The OPXV DNA could not be detected in spleens from any animals tested. Our results indicated that wild animals living in rural human habitation areas of Zambia have been infected with OPXV.

    DOI: 10.1099/vir.0.070219-0

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  • The zoonotic potential of avian influenza viruses isolated from wild waterfowl in Zambia 査読

    Edgar Simulundu, Naganori Nao, John Yabe, Nilton A. Muto, Thami Sithebe, Hirofumi Sawa, Rashid Manzoor, Masahiro Kajihara, Mieko Muramatsu, Akihiro Ishii, Hirohito Ogawa, Aaron S. Mweene, Ayato Takada

    ARCHIVES OF VIROLOGY   159 ( 10 )   2633 - 2640   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER WIEN  

    Whilst remarkable progress in elucidating the mechanisms governing interspecies transmission and pathogenicity of highly pathogenic avian influenza viruses (AIVs) has been made, similar studies focusing on low-pathogenic AIVs isolated from the wild waterfowl reservoir are limited. We previously reported that two AIV strains (subtypes H6N2 and H3N8) isolated from wild waterfowl in Zambia harbored some amino acid residues preferentially associated with human influenza virus proteins (so-called human signatures) and replicated better in the lungs of infected mice and caused more morbidity than a strain lacking such residues. To further substantiate these observations, we infected chickens and mice intranasally with AIV strains of various subtypes (H3N6, H3N8, H4N6, H6N2, H9N1 and H11N9) isolated from wild waterfowl in Zambia. Although some strains induced seroconversion, all of the tested strains replicated poorly and were nonpathogenic for chickens. In contrast, most of the strains having human signatures replicated well in the lungs of mice, and one of these strains caused severe illness in mice and induced lung injury that was characterized by a severe accumulation of polymorphonuclear leukocytes. These results suggest that some strains tested in this study may have the potential to infect mammalian hosts directly without adaptation, which might possibly be associated with the possession of human signature residues. Close monitoring and evaluation of host-associated signatures may help to elucidate the prevalence and emergence of AIVs with potential for causing zoonotic infections.

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  • Detection and characterization of zoonotic pathogens of free-ranging non-human primates from Zambia 査読

    Jesca Nakayima, Kyoko Hayashida, Ryo Nakao, Akihiro Ishii, Hirohito Ogawa, Ichiro Nakamura, Ladslav Moonga, Bernard M. Hang'ombe, Aaron S. Mweene, Yuka Thomas, Yasuko Orba, Hirofumi Sawa, Chihiro Sugimoto

    PARASITES & VECTORS   7   490   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMED CENTRAL LTD  

    Background: Wildlife may harbor infectious pathogens that are of zoonotic concern acting as a reservoir of diseases transmissible to humans and domestic animals. This is due to human-wildlife conflicts that have become more frequent and severe over recent decades, competition for the available natural habitats and resources leading to increased human encroachment on previously wild and uninhabited areas.
    Methods: A total of 88 spleen DNA samples from baboons and vervet monkeys from Zambia were tested for zoonotic pathogens using genus or species-specific PCR. The amplified products were then subjected to sequencing analysis.
    Results: We detected three different pathogenic agents, including Anaplasma phagocytophilum in 12 samples (13.6%), Rickettsia spp. in 35 samples (39.8%) and Babesia spp. in 2 samples (2.3%).
    Conclusion: The continuously increasing contacts between humans and primate populations raise concerns about transmission of pathogens between these groups. Therefore, increased medical and public awareness and public health surveillance support will be required to detect and control infections caused by these agents at the interface between humans and wildlife.

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  • Molecular Epidemiology of Paramyxoviruses in Frugivorous Eidolon helvum Bats in Zambia 査読

    Walter Muleya, Michihito Sasaki, Yasuko Orba, Akihiro Ishii, Yuka Thomas, Emiko Nakagawa, Hirohito Ogawa, Bernard Hang'ombe, Boniface Namangala, Aaron Mweene, Ayato Takada, Takashi Kimura, Hirofumi Sawa

    JOURNAL OF VETERINARY MEDICAL SCIENCE   76 ( 4 )   611 - 614   2014年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC VET SCI  

    In this study, we describe the detection of novel paramyxoviruses from the Eidolon helvum species of fruit bats. We extracted RNA from 312 spleen samples from bats captured in Zambia over a period of 4 years (2008-2011). Semi-nested RT-PCR detected a total of 25 (8%) positive samples for paramyxoviruses which were then directly sequenced and analyzed using phylogenetic analysis. Among the positive samples, seven novel paramyxoviruses were detected. Five viruses were closely related to the genus Henipavirus, while two viruses were related to the unclassified Bat paramyxoviruses from Ghana and Congo Brazzaville. Our study identified novel Henipavirus-related and unrelated viruses using RT-PCR in fruit bats from Kansaka National Park and indicated the presence of similar Bat paramyxoviruses originating from wide geographic areas, suggesting the ability of bats to harbor and transmit viruses. The presence of these viruses in fruit bats might pose a public health risk.

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  • Characterization of the Envelope Glycoprotein of a Novel Filovirus, Lloviu Virus 査読

    Junki Maruyama, Hiroko Miyamoto, Masahiro Kajihara, Hirohito Ogawa, Ken Maeda, Yoshihiro Sakoda, Reiko Yoshida, Ayato Takada

    JOURNAL OF VIROLOGY   88 ( 1 )   99 - 109   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Lloviu virus (LLOV), a novel filovirus detected in bats, is phylogenetically distinct from viruses in the genera Ebolavirus and Marburgvirus in the family Filoviridae. While filoviruses are known to cause severe hemorrhagic fever in humans and/or nonhuman primates, LLOV is biologically uncharacterized, since infectious LLOV has never been isolated. To examine the properties of LLOV, we characterized its envelope glycoprotein (GP), which likely plays a key role in viral tropism and pathogenicity. We first found that LLOV GP principally has the same primary structure as the other filovirus GPs. Similar to the other filoviruses, virus-like particles (VLPs) produced by transient expression of LLOV GP, matrix protein, and nucleoprotein in 293T cells had densely arrayed GP spikes on a filamentous particle. Mouse antiserum to LLOV VLP was barely cross-reactive to viruses of the other genera, indicating that LLOV is serologically distinct from the other known filoviruses. For functional study of LLOV GP, we utilized a vesicular stomatitis virus (VSV) pseudotype system and found that LLOV GP requires low endosomal pH and cathepsin L, and that human C-type lectins act as attachment factors for LLOV entry into cells. Interestingly, LLOV GP-pseudotyped VSV infected particular bat cell lines more efficiently than viruses bearing other filovirus GPs. These results suggest that LLOV GP mediates cellular entry in a manner similar to that of the other filoviruses while showing preferential tropism for some bat cells.

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  • Genome sequence of a Bacillus anthracis outbreak strain from Zambia, 2011 査読

    Naomi Ohnishi, Fumito Maruyama, Hirohito Ogawa, Hirokazu Kachi, Shunsuke Yamada, Daisuke Fujikura, Ichiro Nakagawa, Mudenda B. Hang'ombe, Yuka Thomas, Aaron S. Mweene, Hideaki Higashi

    Genome Announcements   2 ( 2 )   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    In August 2011, an anthrax outbreak occurred among Hippopotamus amphibius hippopotamuses and humans in Zambia. Here, we report the draft genome sequence of the Bacillus anthracis outbreak strain CZC5, isolated from tissues of H. amphibius hippopotamuses that had died in the outbreak area.

    DOI: 10.1128/genomeA.00116-14

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  • Human Parainfluenza Virus Type 3 in Wild Nonhuman Primates, Zambia 査読

    Michihito Sasaki, Akihiro Ishii, Yasuko Orba, Yuka Thomas, Bernard M. Hang'ombe, Ladslav Moonga, Aaron S. Mweene, Hirohito Ogawa, Ichiro Nakamura, Takashi Kimura, Hirofumi Sawa

    EMERGING INFECTIOUS DISEASES   19 ( 9 )   1500 - 1503   2013年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CENTERS DISEASE CONTROL  

    Human parainfluenza virus type 3 (HPIV3) genome was detected in 4 baboons in Zambia. Antibody for HPIV3 was detected in 13 baboons and 6 vervet monkeys in 2 distinct areas in Zambia. Our findings suggest that wild nonhuman primates are susceptible to HPIV3 infection.

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  • Identification of a novel polyomavirus from vervet monkeys in Zambia 査読

    Hiroki Yamaguchi, Shintaro Kobayashi, Akihiro Ishii, Hirohito Ogawa, Ichiro Nakamura, Ladslav Moonga, Bernard M. Hang'ombe, Aaron S. Mweene, Yuka Thomas, Takashi Kimura, Hirofumi Sawa, Yasuko Orba

    Journal of General Virology   94 ( 6 )   1357 - 1364   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To examine polyomavirus (PyV) infection in wildlife, we investigated the presence of PyVs in Zambia with permission from the Zambia Wildlife Authority. We analysed 200 DNA samples from the spleens and kidneys (n5100 each) of yellow baboons and vervet monkeys (VMs) (n550 each). We detected seven PyV genome fragments in 200 DNA samples using a nested broadspectrum PCR method, and identified five full-length viral genomes using an inverse PCR method. Phylogenetic analysis of virally encoded proteins revealed that four PyVs were closely related to either African green monkey PyV or simian agent 12. Only one virus detected from a VM spleen was found to be related, with relatively low nucleotide sequence identity (74 %), to the chimpanzee PyV, which shares 48% nucleotide sequence identity with the human Merkel cell PyV identified from Merkel cell carcinoma. The obtained entire genome of this virus was 5157 bp and had large T- and small t-antigens, and VP1 and VP2 ORFs. This virus was tentatively named vervet monkey PyV 1 (VmPyV1) as a novel PyV. Comparison with other PyVs revealed that VmPyV1, like chimpanzee PyV, had a longer VP1 ORF. To examine whether the VmPyV1 genome could produce viral proteins in cultured cells, the whole genome was transfected into HEK293T cells. We detected VP1 protein expression in the transfected HEK293T cells by immunocytochemical and immunoblot analyses. Thus, we identified a novel PyV genome from VM spleen. © 2013 SGM.

    DOI: 10.1099/vir.0.050740-0

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  • Relatedness between Host Species and Genotype of Beak and Feather Disease Virus Suggesting Possible Interspecies Cross Infection during Bird Trade 査読

    Hirohito Ogawa, Rajesh Chahota, Kenji Ohya, Tsuyoshi Yamaguchi, Hideto Fukushi

    JOURNAL OF VETERINARY MEDICAL SCIENCE   75 ( 4 )   503 - 507   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC VET SCI  

    Beak and feather disease virus (BFDV) is a causative agent of psittacine beak and feather disease (PBFD), which shows a characteristic feather disorder in psittacine birds. In the present study, the subclinical infection rate of PBFD in imported and domestically bred psittacine birds was investigated by polymerase chain reaction. As a result, 126 of 402 birds (31.3%) were found to be BFDV positive. The DNA sequences of the part of open reading frame (ORF) Cl were determined for 16 BFDV-positive randomly selected samples. One of 16 samples was found to have a mixed infection, and 5 different BFDV sequences were obtained from a single African grey parrot. In phylogenic analysis, almost BFDV sequences included in each genetic cluster of phylogenic tree belonged to the same psittacine subfamily. BFDV derived from African grey parrot was closely related to the BFDV derived from cockatoos by way of exception. The natural habitat of the African grey parrot and cockatoos is different, and therefore, the possibility of interspecies cross infection through the bird trade is suggested from the exceptional BFDV sequences.

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  • Human-animal anthrax outbreak in the Luangwa valley of Zambia in 2011 査読

    Mudenda B. Hang'ombe, James C. L. Mwansa, Sergio Muwowo, Phillip Mulenga, Muzala Kapina, Eric Musenga, David Squarre, Liywali Mataa, Suzuki Y. Thomas, Hirohito Ogawa, Hirofumi Sawa, Hideaki Higashi

    TROPICAL DOCTOR   42 ( 3 )   136 - 139   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ROYAL SOC MEDICINE PRESS LTD  

    There has been a reduction of incidences of anthrax in the developed countries but it is still a public health problem in the developing countries where communities live in interface areas with wildlife. An outbreak of anthrax in Hippopotamus amphibious was observed in Zambia. Following the death of hippopotamuses, suspected human cases were reported. The objective of this study was to isolate and confirm Bacillus anthracis and to determine the antimicrobial susceptibility for the management of the disease. Of the specimens collected, 29.4% (95% confidence interval [CI], 11.4-56.0) were from humans, 42.1% (95% CI, 21.1-66.0) were from hippopotamuses and 20.0% (95% CI, 6.61-44.3) from the soil were found to be positive were for B. anthracis. An antimicrobial susceptibility test revealed that all the isolates were found to be sensitive to the recommended antibiotics. The disease control was achieved by case management and by explaining to the communities that they should avoid contact with animals that die from unknown causes.

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  • A Case of Congenital Hyperostosis in a Newborn Piglet 査読

    Koji Uetsuka, Takayuki Suzuki, Hirohito Ogawa, Hiroko Sato, Kunio Doi, Tetsuo Nunoya

    JOURNAL OF VETERINARY MEDICAL SCIENCE   74 ( 2 )   259 - 262   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC VET SCI  

    One female newborn piglet showed prominent thickening of both forelimbs and died soon after birth. Histopathologically, thin and woven trabeculae of bone was extending out from the edge of cortical bone in the affected forelimbs, and diagnosed as congenital hyperostosis. The extent of radially proliferated trabeculae was most prominent in radioulna. Many round- to spindle-shaped cells were observed in periosteum, which were considered to be osteoblasts. Around the periosteum, the mesenchymal proliferation was extensive with abundant mucus, and cartilaginous metaplastic changes were observed mainly around the radioulna and humerus. Dilatation of vessels with fibrin deposition in vessel walls was often observed, which were considered to reflect the localized circulatory disturbance.

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  • Proventricular Dilatation Disease Associated with Avian Bornavirus Infection in a Citron-Crested Cockatoo that Was Born and Hand-Reared in Japan 査読

    Hirohito Ogawa, Yasuyuki Sanada, Naoko Sanada, Megumi Kudo, Kotaro Tuchiya, Toshiaki Kodama, Koji Uetsuka

    JOURNAL OF VETERINARY MEDICAL SCIENCE   73 ( 6 )   837 - 840   2011年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC VET SCI  

    A 5-month-old female Citron-crested Cockatoo (Cacatua sulphurea citrinocristata) that was born and hand-reared in Japan died with suspected proventricular dilatation disease (PDD). Macroscopic and microscopic examinations of the bird revealed characteristic features of PDD, i.e., distention of the proventriculus and infiltration of lymphocytes and plasma cells in ganglia of various organs and in central and peripheral nerves. A linkage of this PDD case to infection with avian bornavirus (ABV) was documented by RTPCR amplification of the virus genomes from the affected bird. Phylogenetic analysis revealed that the ABV identified in this study clustered into the genotype 2, which is one of the dominant ABV genotypes worldwide. To the best of our knowledge, this is the first report of a natural case of PDD associated with ABV infection in Japan.

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  • Detection of all known filovirus species by reverse transcription-polymerase chain reaction using a primer set specific for the viral nucleoprotein gene 査読

    Hirohito Ogawa, Hiroko Miyamoto, Hideki Ebihara, Kimihito Ito, Shigeru Morikawa, Heinz Feldmann, Ayato Takada

    JOURNAL OF VIROLOGICAL METHODS   171 ( 1 )   310 - 313   2011年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The filoviruses, Marburg virus (MARV) and Ebola virus (EBOV), are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates. Sporadic outbreaks of filovirus infection have occurred in Central Africa and parts of Asia. Identification of the natural reservoir animals that are unknown yet and epidemiological investigations are current challenges to forestall outbreaks of filovirus diseases. The filovirus species identified currently include one in the MARV group and five in the EBOV group, with large genetic variations found among the species. Therefore, it has been difficult to develop a single sensitive assay to detect all filovirus species, which would advance laboratory diagnosis greatly in endemic areas. In this study, a highly sensitive universal RT-PCR assay targeting the nucleoprotein (NP) gene of filoviruses was developed. The genomic RNAs of all known MARV and EBOV species were detected by using an NP-specific primer set. In addition, this RT-PCR procedure was verified further for its application to detect viral RNAs in tissue samples of animals infected experimentally and blood specimens of infected patients. This assay will be a useful method for diagnostics and epidemiological studies of filovirus infections. (C) 2010 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jviromet.2010.11.010

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  • A novel genotype of beak and feather disease virus in budgerigars (Melopsittacus undulatus) 査読

    Hirohito Ogawa, Hiroshi Katoh, Naoko Sanada, Yasuyuki Sanada, Kenji Ohya, Tsuyoshi Yamaguchi, Hideto Fukushi

    VIRUS GENES   41 ( 2 )   231 - 235   2010年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Beak and feather disease virus (BFDV) is a causative agent for psittacine beak and feather disease (PBFD), which shows a characteristic feather disorder in psittacine birds. Nineteen budgerigars, which were clinically suspected to have PBFD, were examined by two polymerase chain reactions (PCR), which target each of open reading frames (ORFs) V1 and C1. All of the 19 samples were detected BFDV by the PCR targeting ORF C1, whereas only two of them were detected by the PCR targeting ORF V1. It was assumed that BFDV derived from budgerigar (budgerigar BFDV) has two genotypes, which are tentatively classified as budgerigar BFDV genotype 1 and genotype 2 by the PCR amplification patterns. Whole genome sequences of six budgerigar BFDVs were determined to reveal the existence of two genotypes. In the phylogenic analysis, six budgerigar BFDV sequences formed a unique group branched from the other 23 published BFDV sequences. The budgerigar BFDV genotype 1 and genotype 2 were also segregated each other, and budgerigar BFDV genotype 2 was particularly distantly related with the other BFDVs. These results suggest budgerigar BFDV is a unique in the known BFDVs and is divided into two genotypes.

    DOI: 10.1007/s11262-010-0509-0

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  • A Review of DNA Viral Infections in Psittacine Birds 査読

    Hiroshi Katohi, Hirohito Ogawa, Kenji Ohya, Hideto Fukushi

    JOURNAL OF VETERINARY MEDICAL SCIENCE   72 ( 9 )   1099 - 1106   2010年9月

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    記述言語:英語   出版者・発行元:JAPAN SOC VET SCI  

    To date, several DNA viral infections have been reported in psittacine birds. Psittacine beak and feather disease (PBFD) is characterized by symmetric feather dystrophy and loss and development of beak deformities. PBFD is caused by beak and feather virus, which belongs to the Circoviridae, and is the most important infection in psittacine birds worldwide. Avian polyomavirus infection causes acute death, abdominal distention, and feather abnormalities. Pacheco's disease (PD), which is caused by psittacid herpesvirus type I, is an acute lethal disease without a prodrome. Psittacine adenovirus infections are described as having a clinical progression similar to PD. The clinical changes in psittacine poxvirus-infected birds include serious ocular discharge, rhinitis, and conjunctivitis, followed by the appearance of ulcerations on the medial canthi of the eyes. Internal papillomatosis of parrots (IPP) is a tumor disease characterized by progressive development of papillomas in the oral and cloacal mucosa. IPP has been suggested to caused by papillomavirus or herpesvirus. However, information about these diseases is limited. Here we review the etiology, clinical features, pathology, epidemiology, and diagnosis of these DNA viruses.

    DOI: 10.1292/jvms.10-0022

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  • Prevalence of swine Torque teno virus genogroups 1 and 2 in Japanese swine with suspected post-weaning multisystemic wasting syndrome and porcine respiratory disease complex 査読

    Osamu Taira, Hirohito Ogawa, Aki Nagao, Kotaro Tuchiya, Tetsuo Nunoya, Susumu Ueda

    VETERINARY MICROBIOLOGY   139 ( 3-4 )   347 - 350   2009年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Torque teno virus (TTV) was first isolated from a human hepatitis patient in 1997. TTV was also identified in several animals, including pigs, cattle, sheep, cats and dogs. In this study, we analysed the prevalence of swine TTV genogroups 1 (TTV1) and 2 (TTV2) in Japanese swine populations with suspected post-weaning multisystemic wasting syndrome and porcine respiratory disease by using a nested polymerase chain reaction method. Of 153 serum samples from 16 different herds in Japan, TTVI was detected in 46 samples (30%), TTV2 in 47 samples (31%) and both in 15 samples (10%). There was no significant difference in the detection rate among geographical regions. The overall prevalence rate of TTV genogroups was significantly lower in <= 30-day-old pigs (11%) compared to that in older age groups (54-82%). These results suggest that swine TTV may be widespread in post-weaning pigs and could play aetiological roles in pig diseases in Japan. This is the first report on the prevalence of swine TTV in Japan. (C) 2009 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.vetmic.2009.06.010

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  • Multiplex PCR and multiplex RT-PCR for inclusive detection of major swine DNA and RNA viruses in pigs with multiple infections 査読

    Hirohito Ogawa, Osamu Taira, Takuya Hirai, Hiromi Takeuchi, Aki Nagao, Yoshiki Ishikawa, Kotaro Tuchiya, Tetsuo Nunoya, Susumu Ueda

    JOURNAL OF VIROLOGICAL METHODS   160 ( 1-2 )   210 - 214   2009年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Multiplex PCR and multiplex RT-PCR were developed to identify nine viruses in pigs with multiple infections. These viruses are: porcine circovirus type 2 (PCV2), suid herpesvirus 1, porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), Japanese encephalitis virus, porcine rotavirus A (PoRV-A), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and Getah virus. These methods were shown to be high specificity and sensitivity. In the clinical application, a total of 75 field samples were examined by our methods and previously reported methods for PCV2, PRRSV, TGEV, and PEDV. As a result, the detection rates of our multiplex PCR and multiplex RTPCR were higher than those of the previously reported methods. Furthermore, it was confirmed that 24 PCV2 positive samples were co-infected with other viruses, 11 with PRRSV, 10 with PPV, 2 with PoRV-A, and 1 with TGEV by a combination of multiplex PCR and multiplex RT-PCR. PPV and PoRV-A were newly detected by multiplex PCR and multiplex RT-PCR. These results suggest that the combination of our multiplex PCR and multiplex RT-PCR is useful for rapid and accurate identification of nine major pathogenic viruses in pigs with multiple infections. (C) 2009 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jviromet.2009.05.010

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  • A survey of avian polyomavirus (APV) infection in imported and domestic bred psittacine birds in Japan 査読

    Hirohito Ogawa, Rajesh Chahota, Takayuki Hagino, Kenji Ohya, Tsuyoshi Yamaguchi, Hideto Fukushi

    JOURNAL OF VETERINARY MEDICAL SCIENCE   68 ( 7 )   743 - 745   2006年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC VET SCI  

    Although birds infected with avian polyomavirus (APV) subclinically could be a source of infection, no epidemiological studies of APV in psittacine birds have been reported in Japan. In the present study, we investigated subclinical morbidity rate of APV in imported and domestically bred psittacine birds by polymerase chain reaction (PCR). Of 402 live birds from which blood or feather samples were taken between April, 2003 and March, 2004, 11 (2.7%) were found to be APV positive. The DNA sequences of the APV t/T antigen region were determined for five APV-positive randomly selected samples and were found to be conserved.

    DOI: 10.1292/jvms.68.743

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  • Genetic diversity and epizootiology of Chlamydophila psittaci prevalent among the captive and feral avian species based on VD2 region of ompA gene 査読

    Rajesh Chahota, Hirohito Ogawa, Yoko Mitsuhashi, Kenji Ohya, Tsuyoshi Yamaguchi, Hideto Fukushi

    MICROBIOLOGY AND IMMUNOLOGY   50 ( 9 )   663 - 678   2006年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    To study genetic diversity and occurrence of Chlamydophila psittaci, a total of 1,147 samples from 11 avian orders including 53 genera and 113 species of feral and captive birds were examined using ompA gene based nested PCR. Three types of chlamydiae: C. psittaci (94.12%), C. abortus (4.41%) and unknown Chlamydophila sp. (1.47%) were identified among 68 (5.93%) positive samples (Psittaciformes-59, Ciconiiformes-8 and Passeriformes-1). Based on nucleotide sequence variations in the VD2 region of ompA gene, all 64 detected C. psittaci strains were grouped into 4 genetic clusters. Clusters I, II, III and IV were detected from 57.35%, 19.12%, 10.29% and 7.35% samples respectively. A single strain of unknown Chlamydophila sp. was found phylogenetically intermediate between Chlamydophila species infecting avian and mammalian hosts. Among Psittaciformes, 28 out of 81 tested species including 10 species previously unreported were found to be chlamydiae positive. Chlamydiosis was detected among 8.97% sick and 48.39% dead birds as well 4.43% clinically normal birds. Therefore, it was observed that though various genetically diverse chlamydiae may cause avian chlamydiosis, only a few C. psittaci strains are highly prevalent and frequently associated with clinical/subclinical infections.

    DOI: 10.1111/j.1348-0421.2006.tb03839.x

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  • Duplex shuttle PCR for differential diagnosis of budgerigar fledgling disease and psittacine beak and feather disease 査読

    H Ogawa, T Yamaguchi, H Fukushi

    MICROBIOLOGY AND IMMUNOLOGY   49 ( 3 )   227 - 237   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    Two common viral diseases in psittacine birds including budgerigar fledgling disease (BFD), generally called avian polyomavirus (APV) infection, and psittacine beak and feather disease (PBFD) have similar clinical manifestations characterized by feather disorders. A duplex shuttle PCR was developed for detection of APV and PBFD virus (PBFDV). Two pairs of oligonucleotide primers were designed to amplify a 298-bp fragment of the t/T antigen region of APV genome and a 495-bp fragment of the capsid protein region encoded by open reading frame (ORF) C1 of PBFDV genome, respectively. In the present study, APV and PBFDV were detected simultaneously in one tube by duplex shuttle PCR using these two pairs of primers. The detection limits were 2 viral copies of APV and 3 viral copies of PBFDV. In the clinical application, we detected 16 APV-positive, 15 PBFDV-positive, and 3 mixed infected samples in 39 samples examined. Sequences of the amplified products were read. The t/T antigen region was conserved in the APV-positive samples as expected. ORF C1 of PBFDV genome showed diversity. Phylogenic analysis indicated that PBFDV ORF C1 consisted of 6 clusters which were related to subfamilies of psittacine birds. Our duplex shuttle PCR could be a useful method for differential diagnosis and molecular epidemiology of BFD and PBFD.

    DOI: 10.1111/j.1348-0421.2005.tb03724.x

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  • Regulation of the major immediate early gene promoter by tegument proteins of human herpesvirus-6

    李 昊堃, 難波ひかる, 小川 寛人, 本田 知之

    第68回 日本ウイルス学会学術集会 Program Book   302   2021年11月

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    掲載種別:研究発表ペーパー・要旨(全国大会,その他学術会議)  

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  • ヒトヘルペスウイルス 6Bの新規細胞侵入レセプターの探索

    小川 寛人, 藤倉 大輔, 難波ひかる, 山下 信子, 本田 知之, 山田 雅夫

    第68回 日本ウイルス学会学術集会 Program Book   303   2021年11月

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    担当区分:筆頭著者   掲載種別:研究発表ペーパー・要旨(全国大会,その他学術会議)  

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  • インフルエンザウイルスの細胞侵入に対するセシウム添加の影響(in vitro)

    山下 信子, 小川 寛人, 難波 ひかる, 山田 雅夫

    臨床とウイルス   48 ( 3 )   S91 - S91   2020年9月

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    記述言語:日本語   掲載種別:研究発表ペーパー・要旨(全国大会,その他学術会議)   出版者・発行元:日本臨床ウイルス学会  

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  • 岡山大学自然生命科学研究支援センターに搬入される家畜ブタのE型肝炎ウイルス感染状況について

    小川 寛人, 平山 晴子, 田中 爽暉, 矢田 範夫, 難波 ひかる, 山下 信子, 米満 研三, 前田 健, 樅木 勝巳, 山田 雅夫

    岡山実験動物研究会報   ( 35 )   62 - 63   2019年6月

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    記述言語:日本語   掲載種別:研究発表ペーパー・要旨(全国大会,その他学術会議)   出版者・発行元:岡山実験動物研究会  

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  • 唾液からのHHV6とHHV7のDNA検出率の前方視的検討 保育園入園半年と非就園半年の比較

    鳥越 貞義, 渡辺 正博, 難波 ひかる, 山下 信子, 小川 寛人, 山田 雅夫, 菅 秀, 根来 麻奈美

    臨床とウイルス   47 ( 2 )   S84 - S84   2019年4月

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    記述言語:日本語   掲載種別:研究発表ペーパー・要旨(全国大会,その他学術会議)   出版者・発行元:日本臨床ウイルス学会  

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  • ザンビアのエジプトルーセットオオコウモリから検出されたマールブルグウイルスの遺伝学的解析

    梶原将大, CHANGULA Katendi, HANG’OMBE Bernard, 播磨勇人, 宮本洋子, 衛藤芳樹, 奥谷公亮, 磯野真央, 吉田玲子, 邱永晋, 森(梶原)亜紀奈, 大場靖子, 澤洋文, 澤洋文, 小川寛人, SIMULUNDU Edgar, SQUARRE David, MUKONKA Victor, MWEENE Aaron, 高田礼人, 高田礼人

    日本ウイルス学会学術集会プログラム・予稿集(Web)   67th   2019年

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    掲載種別:研究発表ペーパー・要旨(全国大会,その他学術会議)  

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  • セシウムがインフルエンザウイルス増殖に及ぼす影響

    山下信子, 小川寛人, 難波ひかる, 山田雅夫

    日本ウイルス学会学術集会プログラム・予稿集(Web)   67th   2019年

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    掲載種別:研究発表ペーパー・要旨(全国大会,その他学術会議)  

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  • HHV6Bテグメント蛋白質間相互作用の網羅的解析

    難波ひかる, 小川寛人, 山下信子, 山田雅夫

    日本ウイルス学会学術集会プログラム・予稿集(Web)   67th   2019年

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    掲載種別:研究発表ペーパー・要旨(全国大会,その他学術会議)  

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  • 長鎖型シークエンサーを用いた炭疽菌のゲノム欠失およびDNAメチル化の解析

    古田芳一, 播磨勇人, 伊藤絵美子, 丸山史人, 大西なおみ, 大崎研, 小川寛人, SQUARRE David, OMBE Bernard Hang, 東秀明

    日本細菌学雑誌(Web)   74 ( 1 )   2019年

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    掲載種別:研究発表ペーパー・要旨(全国大会,その他学術会議)  

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  • ザンビアにおけるRousettus aegyptiacusコウモリのフィロウイルス感染の血清有病率 査読 国際誌

    KAJIHARA Masahiro, CHANGULA Katendi, MORI-KAJIHARA Akina, ETO Yoshiki, MIYAMOTO Hiroko, YOSHIDA Reiko, SHIGENO Asako, HANG’OMBE Bernard M, QIU Yongjin, DANIEL Mwizabi, SQUARRE David, OGAWA Hirohito, HARIMA Hayato, SIMULUNDU Edgar, KONDOH Tatsunari, SATO Masahiro, TAKADATE Yoshihiro, MUKONKA Victor, MWEENE Aaron S, TAKADA Ayato, TAKADA Ayato

    日本ウイルス学会学術集会プログラム・抄録集   218 ( suppl_5 )   S312-S317   2018年11月

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  • 歯周病原細菌によるヒトと伴侶動物イヌとの人獣共通感染症検査の研究

    田井 真砂子, 伊東 孝, 平山 晴子, 矢田 範夫, 小川 寛人, 田村 和也, 伊東 有希, 大久保 圭祐, 伊東 昌洋, 中村 心, 岡本 憲太郎, 平井 公人, 山城 圭介, 大森 一弘, 山本 直史, 樅木 勝巳, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   135 - 135   2018年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 養豚農場から大学動物実験施設に搬入される家畜ブタのE型肝炎ウイルス感染状況について

    小川 寛人, 平山 晴子, 田中 爽暉, 矢田 範夫, 難波 ひかる, 山下 信子, 米満 研三, 前田 健, 樅木 勝巳, 山田 雅夫

    日本獣医学会学術集会講演要旨集   161回   475 - 475   2018年8月

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    記述言語:日本語   出版者・発行元:(公社)日本獣医学会  

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  • 動物実験施設に搬入される家畜ブタにおけるE型肝炎ウイルスの遺伝子検出および血清学的解析

    小川 寛人, 難波 ひかる, 山下 信子, 山田 雅夫, 田中 爽暉, 矢田 範夫, 平山 晴子, 樅木 勝巳, 米満 研三, 前田 健

    臨床とウイルス   46 ( 2 )   S58 - S58   2018年4月

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    記述言語:日本語   出版者・発行元:日本臨床ウイルス学会  

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  • フィロウイルスの差次的トロピズムはコウモリNiemann-Pick C1蛋白質の多形により制御される

    TAKADATE Yoshihiro, KONDOH Tatsunari, MARUYAMA Junki, MARUYAMA Junki, MANZOOR Rashid, KURODA Makoto, KURODA Makoto, OGAWA Hirohito, OGAWA Hirohito, SATO Masahiro, FURUYAMA Wakako, FURUYAMA Wakako, YOSHIDA Reiko, IGARASHI Manabu, TAKADA Ayato, TAKADA Ayato

    日本ウイルス学会学術集会プログラム・抄録集   66th   2018年

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  • 西アフリカの大型草食齧歯類グラスカッターと反芻家畜の腸内菌叢比較

    大屋賢司, 片山幸枝, 大松勉, 高島康弘, 澤井宏太郎, 小川寛人, ADENYO Chris, KAYANG Boniface, 水谷哲也, 福士秀人, 村山美穂

    日本獣医学会学術集会講演要旨集   161st   2018年

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  • ストローオオコウモリ(Eidolon helvum)から分離された新規コウモリアデノウイルスの性状解析 査読 国際誌

    OGAWA Hirohito, KAJIHARA Masahiro, NAO Naganori, SHIGENO Asako, FUJIKURA Daisuke, HANG’OMBE Bernard M, MWEENE Aaron S, MUTEMWA Alisheke, SQUARRE David, YAMADA Masao, HIGASHI Hideaki, SAWA Hirofumi, TAKADA Ayato

    日本ウイルス学会学術集会プログラム・抄録集   9 ( 12 )   2017年12月

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  • 西アフリカの大型齧歯類グラスカッターの保有する微生物について

    大屋 賢司, 高島 康弘, 小川 寛人, 大松 勉, 片山 幸枝, 水谷 哲也, 佐藤 梢[大久保], 川端 寛樹, 松本 干城, 中尾 亮, 福士 秀人, Adenyo Christopher, Kayang B. Boniface, 村山 美穂

    日本獣医学会学術集会講演要旨集   160回   527 - 527   2017年8月

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    記述言語:日本語   出版者・発行元:(公社)日本獣医学会  

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  • ザンビア共和国に飛来するストローオオコウモリ(Eidolon helvum)から分離されたアデノウイルスの性状解析と分子疫学的解析

    小川寛人, 小川寛人, 小川寛人, 梶原将大, 直亨則, 丸山隼輝, 上野恵介, 石井秋宏, 石井秋宏, 藤倉大輔, HANG’OMBE Bernard, AARON Mweene, 山田雅夫, 東秀明, 東秀明, 澤洋文, 澤洋文, 高田礼人, 高田礼人

    日本獣医学会学術集会講演要旨集   159th   376 - 376   2016年8月

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    記述言語:日本語   出版者・発行元:(公社)日本獣医学会  

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  • 北海道大学人獣共通感染症リサーチセンターザンビア拠点における感染症対策への取り組み

    小川寛人, 喜田宏

    最新医学   70 ( 4 )   2015年

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  • Lloviu virusの表面糖タンパク質GPの性状解析

    丸山隼輝, 宮本洋子, 吉田玲子, 前田健, 小川寛人, 迫田義博, 高田礼人

    日本ウイルス学会学術集会プログラム・抄録集   61st   164   2013年10月

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    記述言語:日本語  

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  • 霊長類動物からの新規ポリオーマウイルスの検出

    山口宏樹, 小林進太郎, 石井秋宏, 小川寛人, 木村享史, 澤洋文, 大場靖子

    日本分子生物学会年会プログラム・要旨集(Web)   36th   2013年

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  • フィロウイルスの生態

    梶原将大, 小川寛人, 高田礼人

    実験医学   2013年

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  • ザンビアの野生動物におけるオルソポックスウイルス感染の疫学調査

    大場靖子, 石井秋宏, トーマス 由佳, 小川寛人, 中村一郎, 木村享史, 森川茂, 西條政幸, 澤洋文

    日本ウイルス学会学術集会プログラム・抄録集   60th   443   2012年10月

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  • 霊長類動物におけるパラインフルエンザウイルス3型の疫学調査

    佐々木道仁, 石井秋宏, 大場靖子, THOMAS Yuka, 小川寛人, 中村一郎, 木村享史, 澤洋文, 澤洋文

    日本分子生物学会年会プログラム・要旨集(Web)   35th   2012年

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  • ザンビアにおける霊長類動物からの新規ポリオーマウイルスの検出

    山口宏樹, 山口宏樹, 大場靖子, 小林進太郎, 小林進太郎, 石井秋宏, 小川寛人, 中村一郎, THOMAS由佳, 木村享史, 澤洋文, 澤洋文

    日本ウイルス学会学術集会プログラム・抄録集   60th   2012年

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  • 霊長類動物におけるパラインフルエンザウイルスの疫学調査

    佐々木道仁, 石井秋宏, 大場靖子, トーマス由佳, 小川寛人, 中村一郎, 木村享史, 澤洋文, 澤洋文

    日本ウイルス学会学術集会プログラム・抄録集   60th   2012年

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  • ザンビア共和国の霊長類動物におけるポリオーマウイルスの疫学調査

    山口宏樹, 小林進太郎, 大場靖子, 石井秋宏, 小川寛人, Thomas由佳, 木村享史, 澤洋文

    日本獣医学会学術集会講演要旨集   152nd   2011年

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  • 新生豚にみられた先天性過骨症の一例

    上塚浩司, 鈴木敬之, 富岡ひとみ, 小川寛人, 金子寛子, 土井邦雄, 布谷鉄夫

    日本獣医学会学術集会講演要旨集   148th   2009年

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  • 豚エンテロウイルス性脳脊髄炎

    小川 寛人

    日生研たより   55 ( 6 )   2009年

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  • 鳥ポリオーマウイルスの神経病原性関連因子に関する遺伝子学的解析

    加藤大志, 小川寛人, 細川美里, 大屋賢司, 大屋賢司, 宇根有美, 山口剛士, 山口剛士, 福士秀人, 福士秀人

    日本獣医学会学術集会講演要旨集   142nd   2006年

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  • セキセイインコにおけるオウム・インコ類の嘴-羽毛病ウイルス(BFDV)の新しい遺伝子型と病態について

    小川寛人, 真田直子, 真田靖幸, 加藤大志, 萩野貴之, 米丸加余子, 酒井洋樹, 大屋賢司, 櫻井徳磨, 山口剛士, 山口剛士, 柵木利昭, 福士秀人, 福士秀人

    日本獣医学会学術集会講演要旨集   141st   2006年

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  • ヒトおよび鳥類より分離されたChlamydophila psittaci 株の特性

    Chahota Rajesh, 小川寛人, 大屋賢司, 松本明, 山口剛士, 福士秀人

    獣医畜産新報   59 ( 4 )   289 - 291   2006年

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  • オウム目鳥類の背景病変に関する病理組織学的研究

    高須伸二, 柳井徳磨, 米丸加余子, 小川寛人, 酒井洋樹, 福士秀人, 柵木利昭

    日本獣医学会学術集会講演要旨集   139th   180   2005年3月

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    記述言語:日本語  

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  • 日本の愛玩鳥および展示鳥類におけるオウム病クラミジアの感染状況

    CHAHOTA Rajesh, 大屋賢司, 小川寛人, 山口剛士, 山口剛士, 福士秀人, 福士秀人

    日本獣医学会学術集会講演要旨集   140th   2005年

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  • 国内外由来オウム類におけるオウム・インコ類のくちばし-羽毛病(PBFD)の感染状況およびPBFDウイルス(PBFDV)の系統解析

    小川寛人, RAJESH Chahota, 萩野貴之, 大屋賢司, 山口剛士, 福士秀人

    日本獣医学会学術集会講演要旨集   140th   2005年

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  • 愛玩鳥におけるクラミジア症の3例

    高須伸二, 柳井徳磨, 小川寛人, 福士秀人, 酒井洋樹, 柵木利昭

    日本獣医学会学術集会講演要旨集   136th   111   2003年9月

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    記述言語:日本語  

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  • 愛玩鳥のウイルスおよびクラミジア感染症の疫学

    小川寛人, 寺崎香織, 道越小雪, 山田荘一, 山口剛士, 酒井洋樹, 柳井徳磨, 柵木利昭, 福士秀人

    日本獣医学会学術集会講演要旨集   136th   2003年

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受賞

  • ポスター賞

    2021年11月   第68回日本ウイルス学会学術集会   ヒトヘルペスウイルス 6Bの新規細胞侵入レセプターの探索

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  • Best Poster Award

    2009年10月   4th Congress of APVS 2009 in Tsukuba, Asian Pig Veterinary Society 2009,  

    小川 寛人

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  • Poster Contest 2nd Place

    2006年8月   27th AAV Annual Conference & Expo, Association of Avian Veterinarians,  

    小川 寛人

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共同研究・競争的資金等の研究

  • ヒトヘルペスウイルス6B新規細胞侵入レセプターの探索

    2019年04月 - 2022年03月

    文部科学省  科学研究補助金(基盤研究(C)) 

    小川 寛人

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    担当区分:研究代表者  資金種別:競争的資金

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  • 果食コウモリから分離された新規アデノウイルス(EhAdV)の初期遺伝子領域の機能解析および新規ベクター開発に向けた基盤研究

    2018年12月

    公益財団法人岡山医学振興会  第18回研究助成 

    小川 寛人

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    担当区分:研究代表者  資金種別:競争的資金

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  • ガーナ共和国のブタにおけるフィロウイルスの血清疫学調査

    2017年04月 - 2018年03月

    北海道大学人獣共通感染症リサーチセンター  平成29年度一般共同研究 

    小川 寛人

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    担当区分:研究代表者  資金種別:競争的資金

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  • ヒトヘルペスウイルス6Bの伝播機構の解明

    2016年06月 - 2019年

    武田科学振興財団  医学系研究奨励(感染症) 

    小川 寛人

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    担当区分:研究代表者  資金種別:競争的資金

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  • レストンエボラウイルスはアジアのウイルスか?-南部アフリカにおける疫学調査

    2015年04月 - 2018年03月

    文部科学省  科学研究費補助金(若手研究(B)) 

    小川 寛人

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    担当区分:研究代表者  資金種別:競争的資金

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  • A New Genotype of PBFD Virus in Budgerigars

    2006年

    (財)加藤記念バイオサイエンス研究振興財団  第18回(2006年度)加藤記念国際交流助成 

    小川 寛人

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    担当区分:研究代表者  資金種別:競争的資金

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担当授業科目

  • ウイルス学 (2021年度) 特別  - その他

  • ウイルス学実習 (2021年度) 特別  - その他

  • 感染症と戦う (2021年度) 第2学期  - 木1~2

  • 病原ウイルス学I(演習・実習) (2021年度) 特別  - その他

  • 病原ウイルス学I(講義・演習) (2021年度) 特別  - その他

  • 病原ウイルス学II(演習・実習) (2021年度) 特別  - その他

  • 病原ウイルス学II(講義・演習) (2021年度) 特別  - その他

  • ウイルス学 (2020年度) 特別  - その他

  • ウイルス学実習 (2020年度) 特別  - その他

  • 感染症と戦う (2020年度) 第2学期  - 木1,木2

  • 病原ウイルス学I(演習・実習) (2020年度) 特別  - その他

  • 病原ウイルス学I(講義・演習) (2020年度) 特別  - その他

  • 病原ウイルス学II(演習・実習) (2020年度) 特別  - その他

  • 病原ウイルス学II(講義・演習) (2020年度) 特別  - その他

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