2021/08/19 更新

写真a

ナス ヤストモ
那須 保友
NASU Yasutomo
所属
本部 理事
職名
理事
外部リンク

学位

  • 医学博士

  • 医学博士 ( 岡山大学 )

研究分野

  • ライフサイエンス / 泌尿器科学

所属学協会

▼全件表示

委員歴

  • 特定非営利活動法人せとうち臨床遺伝研究会   監事  

    2020年12月 - 現在   

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  • 第4回日本地域医療連携システム学会   会長  

    2020年12月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会財務委員会   委員  

    2020年11月 - 現在   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会会則検討委員会   委員長  

    2020年11月 - 現在   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会倫理・COI委員会   委員  

    2020年11月 - 現在   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会総会賞選考委員会   委員長  

    2020年11月 - 現在   

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    団体区分:学協会

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  • 第34回日本泌尿器内視鏡学会総会   会長  

    2020年11月   

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    団体区分:学協会

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  • 日本学術会議   連携会員  

    2020年10月 - 現在   

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  • 第7回泌尿器画像診断・治療技術研究会   会長  

    2019年9月   

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    団体区分:学協会

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  • (公財)岡山医学振興会   監事  

    2019年6月 - 現在   

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  • (公財)ウエスコ学術振興財団   選考委員  

    2019年6月 - 現在   

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  • 岡山県医用工学研究会   顧問  

    2019年4月 - 現在   

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  • 日本泌尿器科学会知的財産管理委員会   委員  

    2019年4月 - 2021年12月   

      詳細を見る

    団体区分:学協会

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  • 日本泌尿器科学会 監事  

    2019年4月 - 2021年4月   

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    団体区分:学協会

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  • 日本泌尿器科学会医療賞選考委員会   委員  

    2019年4月 - 2021年4月   

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    団体区分:学協会

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  • 日本泌尿器腫瘍学会広報委員会   委員  

    2019年4月 - 2021年3月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会医工連携・新技術検討委員会   委員  

    2018年11月 - 現在   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会Innovation Award選考委員会   副委員長  

    2018年11月 - 現在   

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    団体区分:学協会

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  • 医薬品医療機器総合機構 科学委員会専門部会   委員  

    2018年11月 - 2020年10月   

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  • 認定NPO法人岡山医師研修支援機構   理事  

    2018年6月 - 現在   

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  • 日本泌尿器科学会専門医制度審議会専門研修委員会   委員  

    2018年6月 - 現在   

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    団体区分:学協会

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  • 日本泌尿器科学会専門医制度審議会   委員  

    2018年4月 - 現在   

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    団体区分:学協会

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  • 第4回日本泌尿器癌局所療法研究会   会長  

    2018年2月   

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    団体区分:学協会

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  • 文部科学省地域イノベーション・エコシステム形成プログラム   山口地域事業アドバイザー  

    2017年12月   

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  • 第23回日本遺伝子細胞治療学会   会長  

    2017年7月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会泌尿器腹腔鏡ガイドライン作成委員会   小委員長  

    2017年5月 - 2020年11月   

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    団体区分:学協会

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  • 泌尿器科紀要編集委員会   委員  

    2017年4月 - 現在   

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  • 日本泌尿器腫瘍学会総務/広報委員会   委員  

    2017年4月 - 2019年3月   

      詳細を見る

    団体区分:学協会

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  • 日本泌尿器内視鏡学会ロボット支援手術委員会   委員  

    2016年11月 - 現在   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会学術委員会   副委員長  

    2016年11月 - 2020年11月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会財務委員会   委員長  

    2016年11月 - 2020年11月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会倫理・COI委員会   副委員長  

    2016年11月 - 2020年11月   

      詳細を見る

    団体区分:学協会

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  • 日本泌尿器内視鏡学会総会賞選考委員会   委員  

    2016年11月 - 2020年11月   

      詳細を見る

    団体区分:学協会

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  • 日本泌尿器内視鏡学会総務委員会   委員  

    2016年11月 - 2020年11月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会阿曽賞選考委員会   委員  

    2016年11月 - 2018年11月   

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    団体区分:学協会

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  • ARO協議会   理事  

    2016年8月 - 現在   

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  • 日本泌尿器内視鏡学会   代議員  

    2016年5月 - 現在   

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    団体区分:学協会

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  • 高知大学   非常勤講師  

    2016年5月 - 2021年7月   

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  • 日本泌尿器腫瘍学会 代議員  

    2016年4月 - 現在   

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  • ARO協議会TRセンター運営戦略連絡会   幹事  

    2016年4月 - 現在   

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  • 日本泌尿器科学会学会賞・坂口賞選考委員会   委員  

    2016年4月 - 2018年4月   

      詳細を見る

    団体区分:学協会

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  • 日本遺伝子治療学会財務委員会   委員  

    2015年10月 - 現在   

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    団体区分:学協会

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  • 日本性機能学会 代議員  

    2015年9月 - 現在   

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  • 西日本泌尿器科   編集委員  

    2015年7月 - 2022年3月   

      詳細を見る

    団体区分:学協会

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  • 大阪大学第二特定認定再生医療等委員会   委員  

    2015年6月 - 現在   

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  • 西日本泌尿器科学会 理事  

    2015年6月 - 2022年3月   

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  • 日本泌尿器科学会国際委員会   委員  

    2015年4月 - 2021年4月   

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    団体区分:学協会

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  • 日本泌尿器科学会男女共同参画委員会   委員  

    2015年4月 - 2019年4月   

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    団体区分:学協会

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  • 日本泌尿器科学会生殖・ゲノム委員会   委員  

    2015年4月 - 2019年4月   

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    団体区分:学協会

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  • 日本泌尿器科学会利益相反委員会   委員  

    2015年4月 - 2019年4月   

      詳細を見る

    団体区分:学協会

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  • 日本泌尿器科学会選挙管理委員会   委員  

    2015年4月 - 2017年4月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会プロクター認定制度委員会   委員  

    2014年11月 - 2016年11月   

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    団体区分:学協会

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  • 特定非営利活動法人臨床研究推進ネットワークジャパン   理事  

    2014年4月 - 2020年3月   

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  • 第3回日本泌尿器病理研究会   会長  

    2014年3月   

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    団体区分:学協会

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  • 医薬品医療機器総合機構   専門委員  

    2013年10月 - 現在   

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  • 日本泌尿器内視鏡学会 事務局長  

    2012年11月 - 2016年11月   

      詳細を見る

  • 日本泌尿器内視鏡学会阿曽賞選考委員会   委員長  

    2012年11月 - 2016年11月   

      詳細を見る

    団体区分:学協会

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  • 日本泌尿器内視鏡学会編集委員会   委員  

    2012年11月 - 2016年11月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会ロボット支援手術部会医事小委員会   委員長  

    2012年11月 - 2016年11月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会ロボット支援手術部会   副部会長  

    2012年11月 - 2016年11月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会将来検討委員会   副委員長  

    2012年11月 - 2016年11月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会学術委員会   委員長  

    2012年11月 - 2014年11月   

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    団体区分:学協会

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  • 日本泌尿器科学会 代議員(西部)  

    2012年10月 - 現在   

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  • 日本遺伝子細胞治療学会 理事  

    2011年7月 - 現在   

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  • 厚生労働省 厚生科学審議会   専門委員  

    2011年2月 - 2021年6月   

      詳細を見る

    団体区分:政府

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  • 日本泌尿器内視鏡学会 理事  

    2010年10月 - 現在   

      詳細を見る

  • 日本泌尿器内視鏡学会将来検討委員会   委員長  

    2010年10月 - 2012年11月   

      詳細を見る

    団体区分:学協会

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  • 泌尿器科分子・細胞研究会 世話人  

    2010年4月 - 現在   

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  • (独)理化学研究所分子イメージング科学研究センターマイクロドーズ臨床試験推進検討委員会   委員  

    2010年2月 - 2011年7月   

      詳細を見る

  • 科学研究費委員会   専門委員  

    2009年12月 - 現在   

      詳細を見る

  • 西日本泌尿器科学会 評議員  

    2008年11月 - 現在   

      詳細を見る

  • 日本泌尿器内視鏡学会学術委員会   委員  

    2006年10月 - 2010年10月   

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    団体区分:学協会

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  • 日本泌尿器内視鏡学会広報委員会   委員  

    2004年11月 - 2012年11月   

      詳細を見る

    団体区分:学協会

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  • 日本泌尿器内視鏡学会国際委員会   委員  

    2004年11月 - 2012年11月   

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    団体区分:学協会

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▼全件表示

 

論文

  • Comparison of inflammation-based prognostic scores as predictors of survival outcomes in patients with germ cell tumors

    Yoshinaga K, Sadahira T, Maruyama Y, Mitsui Y, Iwata T, Wada K, Araki M, Watanabe T, Nasu Y

    Investing Cline Urol   62 ( 1 )   47 - 55   2021年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Nationwide surveillance of bacterial pathogens isolated from patients with acute uncomplicated cystitis in 2018: Conducted by the Japanese Research Group for Urinary Tract Infections (JRGU)

    Wada K, Yokoyama T, Uno S, Araki M, Sadahira T, Maruyama Y, Acosta H, Nakajima H, Hiyama Y, Kunishima Y, Togo Y, Nukaya T, Yamada H, Shigemura K, Ito S, Tanimura M, Kobayashi K, Kitano H, Teishima J, Yasuda M, Uehara S, Hamasuna R, Watanabe T, Nakagawa T, Hayami H, Miyazaki J, Takahashi S, Masumori N, Ishikawa K, Kiyota H, Fujisawa M, Arakawa S, Nasu Y, Yamamoto S

    J Infect Chemother   1169 ( 1180 )   2021年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • A second opinion pathology review improves the diagnostic concordance between prostate cancer biopsy and radical prostatectomy specimens

    Maehara T, Sadahira T, Maruyama Y, Wada K, Araki M, Watanabe M, Watanabe T, Yanai H, Nasu Y

    UROLOGY ANNALS   13 ( 2 )   119 - 124   2021年6月

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    記述言語:英語  

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  • A Cace of Metastatic Fumarate Hydratase-Deficient-like Renal Cell Carcinoma Successfully Managed by Ipilimumab plus Nivolumab (Ipi/Nivo)

    Sekito T, Takamoto A, Kobayashi Y, Mitsui M, Watari S, Kubota R, Sadahira T, Iwata T, Nishimura S, Edamura K, Sako T, Araki M, Watanabe M, Watanabe T, Shibata R, Ennishi D, Nasu Y

    Acta Med. Okayama   75 ( 3 )   397 - 402   2021年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Relevance of CYP3A5 Expression on the Clinical Outcome of Patients With Renal Cell Carcinoma

    MatsumotoJ, Kotera Y, Watari S, Takeuchi K, Ueki H, Koyama T, Wada K, Fujiyoshi M, Nasu Y, Ariyoshi N

    ANTICANCER RESEARCH   41 ( 5 )   2511 - 2521   2021年5月

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    記述言語:英語  

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  • Efficacy of holmium laser enucleation in patients with a small(less than 30ml)prostate volume

    Tsuboi I, Maruyama Y, Sadahira T, Wada K, Ando N, Nishimura Y, Araki M, Kurashige T, Ichikawa T, Arata R, Ono N, Watanabe T, Hayata S, Shiina H, Nasu Y

    Investig Clin Urol   62 ( 3 )   298 - 304   2021年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Robot-assisted partial nephrectomy versus standard laparoscopic partial nephrectomy for renal hilar tumor: A prospective multi-institutional study

    Hinata N, Shiroki R, Tanabe K, Eto M, Takenaka A, Kawakita M, Hara I, Hongo F, Ibuki N, Nasu Y, Teishima J, Kawai N, Kawauchi A, Kondo T, Kawamorita N, Oyama C, Horie S, Shimbo M, Kato M, Kanayama H, Koito Y, Fujisawa M

    Int J Urol   28 ( 4 )   382 - 389   2021年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Dual-Functional PLGA Nanoparticles Co-Loaded with Indocyanine Green and Resiquimod for Prostate Cancer Treatment

    Lin W, Li C, Xu N, Watanabe M, Xue R, Xu A, Araki M, Sun R, Liu C, Nasu Y, Huang P

    Int J Nanomedicine   16   2775 - 2787   2021年4月

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    記述言語:英語  

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  • Photodynamic diagnostic ureteroscopy using the VISERA ELITE video system for diagnosis of upper-urinary tract urothelial carcinoma: a prospective cohort pilot study

    Wada K, Araki M, Tanimoto R, Sadahira T, Watari S, Maruyama Y, Mitsui Y, Nakajima H, Acosta H, Katayama S, Iwata T, Nisimura S, Takamoto A, SakoT, Edamura K, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    BMC Urol   21 ( 1 )   45   2021年3月

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    記述言語:英語  

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  • Long-term ureteroscopic management of upper tract urothelial carcinoma: 28-year single-centre experience

    Maruyama Y, Araki M, Wada K, Yoshinaga K, Mitsui Y, Sadahira T, Nishimura S, Edamura K, Kobayashi Y, Watanabe M, Watanabe T, Monga M, Nasu Y, Kumon H

    Jpn J Clin Oncol   51 ( 1 )   130 - 137   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Comparison of intracorporeal versus extracorporeal urinary diversion after robot-assisted radical cystectomy at a medium-sized facility

    Iwata T, Kobayashi Y, Maruyama Y, Kawad T, Sadahira T, Oiwa Y, Katayama S, Nishimura S, Takamoto A, SakoT, Wada K, Edamura K, Araki M, Watanabe M, Watanabe T, Nasu Y

    Int J Clin Oncol   2021年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • The role of Wnt signaling in male reproductive physiology and pathology

    Xue R, Lin W, Sun J, Watanabe M, Xu A, Araki M, Nasu Y, Tang Z, Huang P

    molecular human reproduction   27 ( 1 )   2021年

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    記述言語:英語  

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  • Feasible kidney donation with living marginal donors, including diabetes mellitus

    Yoshinaga K, Araki M, Wada K, Sekito T, Watari S, Maruyama Y, Mitsui Y, Sadahira T, Kubota R, Nishimura S, Edamura K, Kobayashi Y, Tanabe K, Takeuchi H, Kitagawa M, Kitamura S, Wada J, Watanabe M, Watanabe T, Nasu Y

    lmmun lnflamm Dis   9 ( 3 )   1061 - 1068   2021年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Prognostic value of the systemic immune‑infammation index in non‑muscle invasive bladder cance

    Katayama S, Mori K, Pradere B, Laukhtina E, SchuettfortQuhal F, Motlagh RS, Mostafaei H, Grossmann NC, Rajwa P, Moschini M, Mathieu R, Abufaraj M, D'Andrea D, Comperat E, Haydter M, Egawa S, Nasu Y, Shariat SF

    World Journal of Urology   2021年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Discovery and Validation of Nitroxoline as a Novel STAT3 Inhibitor in Drug-resistant Urothelial Bladder Cancer

    Lin W, Sun J, Sadahira T, Xu N, Wada K, Liu C, Araki M, Xu A, Watanabe M, Nasu Y, Huang P

    Int. J. Biol. Sci   17 ( 12 )   3255 - 3267   2021年

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    記述言語:英語  

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  • Efficacy of Lactobacillus vaginal suppositories for the prevention of recurrent cystitis: A phase II clinical trial

    Sadahira T, Wada K, Araki M, Mitsuhata R, Yamamoto M, Maruyama Y, Iwata T, Watanabe M, Watanabe T, Kariyama R, Nasu Y, Ishii A

    INTERNATIONAL JOURNAL OF UROLOGY   2021年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Combined Laparoscopic and CT Monitoring of the Ice-Ball Margin during Cryoablation for Renal Cell Carcinoma Associated with von Hippel-Lindau

    Sekito T, Araki M, Hiraki T, Uka M, Komaki T, Matsui Y, Iguchi T, Katayama S, Yoshinaga K, Watari S, Maruyama Y, Mitsui Y, Kubota R, Sadahira T, Nishimura S, Wada K, Takamoto A, Edamura K, Sako T, Kobayashi Y, Watanabe T, Kanazawa S, Nasu Y

    Acta Med. Okayama   74 ( 5 )   443 - 448   2020年10月

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  • Low-dose rituximab induction therapy is effective in immunological high-risk renal transplantation without increasing cytomegalovirus infection

    Yoshinaga K, Araki M, Wada K, Maruyama Y, Mitsui Y, Sadahira T, Kubota R, Nishimura S, Kobayashi Y, Takeuchi H, Tanabe K, Kitagawa M, Morinaga H, Uchida HA, Kitamura S, Sugiyama H, Wada J, Watanabe M, Watanabe T, Nasu Y

    Int J Urol   27 ( 12 )   1136 - 1142   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Aspects of gene therapy products using current genome-editing technology in Japan

    Yamaguchi T, Uchida E, Okada T, Ozawa K, Onodera M, Kume A, Shimada T, Takahashi S, Tani K, Nasu Y, Mashimo T, Mizuguchi H, Mitani K, Maki K

    Human Gene Therapy   31 ( 19and20 )   1043 - 1053   2020年10月

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    記述言語:英語  

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  • Correlation between lumbar skeletal muscle size and urinary incontinence after radical prostatectomy

    Mitsui Y, Sadahira T, Watanabe T, Araki M, Maruyama Y, Sato R, Rodrigo A.G.H, Wada K, Watanabe M, Chancellor M.B, Nasu Y

    LUTS-Low Urin Tract Symptoms   12 ( 3 )   245 - 252   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Tumor suppressor REIC/Dkk-3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport

    Experimental and Therapeutic Medicine   20 ( 2 )   1739 - 1745   2020年8月

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    記述言語:英語  

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  • Nitroxoline inhibits bladder cancer progression by reversing EMT process and enhancing anti-tumor immunity

    Xu N, Lin W, Sun J, Sadahira T, Xu A, Watanabe M, Guo K, Araki M, Li G, Liu C, Nasu Y, Huang P

    Journal of Cancer   11 ( 22 )   6633 - 6641   2020年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Impact of sarcopenia on longitudinal erectile functional outcomes after nerve-sparing robot assisted radical prostatectomy

    Mitsui Y, Sadahira T, Maruyama Y, Araki M, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    Eur Urol Open Sci   19 ( Suppl 2 )   e1112   2020年7月

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    記述言語:英語  

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  • The second opinion pathology review improves concordance between prostate biopsy and radical prostatectomy specimens

    Maruyama Y, Sadahira T, Araki M, Mitsui Y, Wada K, Edamura K, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    Eur Urol Open Sci   19 ( Suppl 2 )   e1556   2020年7月

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    記述言語:英語  

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  • Preventive efficacy and safety of lactobacillus vaginal suppositories in women with recurrent cystitis: A phase 2 study

    Sadahira T, Wada K, Ishii A, Maruyama Y, Iwata T, Araki M, Watanabe M, Watanabe T, Nasu Y

    Eur Urol Open Sci   19 ( Suppl 2 )   e2036   2020年7月

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    記述言語:英語  

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  • Mixed 20‑peptide cancer vaccine in combination with docetaxel and dexamethasone for castration‑resistant prostate cancer: a randomized phase II trial

    Noguchi M, Arai G, Egawa S, Ohyama C, Naito S, Matsumoto K, Uemura H, Nakagawa M, Nasu Y, Eto M, Suekane S, Sasada T, Shichijo S, Yamada A, Kakuma T, Itoh K

    Cancer Immunol Immunother   69 ( 5 )   847 - 857   2020年5月

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    記述言語:英語  

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  • Factors predicting pathological upgrading after prostatectomy in patients with Gleason grade group 1 prostate cancer based on opinion-matched biopsy specimens. 査読 国際誌

    Yuki Maruyama, Takuya Sadahira, Motoo Araki, Yosuke Mitsui, Koichiro Wada, Acosta Gonzalez Herik Rodrigo, Kazuaki Munetomo, Yasuyuki Kobayashi, Masami Watanabe, Hiroyuki Yanai, Toyohiko Watanabe, Yasutomo Nasu

    Molecular and clinical oncology   12 ( 4 )   384 - 389   2020年4月

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    記述言語:英語  

    The present study investigated the concordance between Gleason scores assigned to prostate biopsy specimens by outside pathologists and a urological pathology expert, and determined the risk of upgrading between opinion-matched Gleason grade group (GGG) 1 biopsy specimens and radical prostatectomy specimens. Between January 2012 and May 2018, 733 patients underwent robot-assisted radical prostatectomy. Patients whose original biopsy specimens from outside hospitals were reviewed by a urological pathology expert Okayama University Hospital were included. Patients who had received neoadjuvant hormonal therapy were excluded. Logistic regression analysis was used to identify predictors of upgrading among GGG 1 diagnoses. A total of 403 patients were included in the present study. Agreement in GGG between initial and second-opinion diagnoses was present in 256 cases (63.5%). Although opinion-matched cases improved concordance between biopsy and prostatectomy specimen GGG compared with single-opinion cases (initial, 35.2%; second-opinion, 36.5%; matched, 41.4%), 71% (56/79) of cases classified as GGG 1 were upgraded after prostatectomy. Multivariate analysis revealed that prostate-specific antigen density and Prostate Imaging Reporting and Data System version 2 score were significant predictors of upgrading (odds ratio, 1.10; P=0.01; and odds ratio, 1.88; P=0.03, respectively). In conclusion, the GGG concordance rate between needle-core biopsy and radical prostatectomy specimens was higher in opinion-matched cases; however, 71% of opinion-matched GGG1 cases were upgraded after robot-assisted radical prostatectomy. Urologists should propose treatment strategies or further biopsy rather than active surveillance for patients with GGG1 and a high PSAD and/or PI-RADS score.

    DOI: 10.3892/mco.2020.1996

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  • Quality of Life and Mental Satisfaction Improve Slowly in Preemptive Kidney Transplantation Compared With Nonpreemptive Kidney Transplantation. 査読 国際誌

    Yosuke Mitsui, Motoo Araki, Yuki Maruyama, Kasumi Yoshinaga, Takuya Sadahira, Koichiro Wada, Katsuyuki Tanabe, Masashi Kitagawa, Yasuyuki Kobayashi, Masami Watanabe, Toyohiko Watanabe, Yasutomo Nasu

    Transplantation proceedings   2020年3月

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    記述言語:英語  

    BACKGROUND AND AIMS: Preemptive kidney transplantation (PEKT) is recognized as the best therapy to avoid dialysis. However, it is not clear whether PEKT recipients experience an improvement in quality of life (QoL) after kidney transplantation (KT) that exceeds that of non-PEKT recipients, since PEKT recipients have not experienced the heavy burden of dialysis. The aim of this study was to compare the changes in QoL for PEKT and non-PEKT recipients following transplantation. METHODS: Patients included in this study underwent living donor KT in our hospital. We excluded patients with incomplete SF-36 scores and with factors that could affect QoL, such as complications or rejection. QoL was assessed by the Short Form 36-Item Health Survey version 2.0 preoperatively and 3 and 12 months postoperatively. RESULTS: Eighty-eight patients underwent living donor KT in our hospital. Twelve PEKT and 20 non-PEKT recipients were enrolled in this retrospective study. In the non-PEKT group, both the physical and mental domain scores dramatically improved from baseline at 3 months, and remained at a similar level at 12 months. In contrast, in the PEKT group, only 1 domain of the physical and mental score improved at 3 months, and the social functioning score gradually improved at 12 months. Although the mental component score showed significant improvement in the non-PEKT group, it did not change in the PEKT group. CONCLUSIONS: The improvement of QoL after transplantation is more evident in the non-PEKT group. PEKT recipients have less mental satisfaction than non-PEKT recipients.

    DOI: 10.1016/j.transproceed.2020.01.042

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  • Differentiation of small (≤ 4 cm) renal masses on multiphase contrast-enhanced CT by deep learning

    Tanaka T, Huang Y, Marukawa Y, Tsuboi Y, Masaoka Y, Kojima K, Iguchi T, Hraki T, Gobara H, Yanai H, Nasu Y, Kanazawa S

    AJR Am20 J Roentgenol   214 ( 3 )   605 - 612   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Robotic Renal Autotransplantation: A Feasibility Study in a Porcine Model. 査読

    Risa Kubota, Motoo Araki, Koichiro Wada, Kasumi Kawamura, Yuki Maruyama, Yosuke Mitsui, Takuya Sadahira, Yuichi Ariyoshi, Takehiro Iwata, Shingo Nishimura, Atsushi Takamoto, Tomoko Sako, Kohei Edamura, Yasuyuki Kobayashi, Yuzuki Kano, Masashi Kitagawa, Katsuyuki Tanabe, Hitoshi Sugiyama, Jun Wada, Masami Watanabe, Toyohiko Watanabe, Yasutomo Nasu

    Acta medica Okayama   74 ( 1 )   53 - 58   2020年2月

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    記述言語:英語  

    We investigated the feasibility of robotic renal autotransplantation (RAT) in a porcine model to reduce invasiveness of RAT. Five pigs underwent robotic RAT using the da Vinci® robotic system. A robotic left nephrectomy was performed in all cases. Robotic RAT was performed on the left side in all but one case. Four ports were used. In 3 cases, the kidney was taken out through the GelPort® and irrigated on ice with Ringer's solution. In 2 cases, a complete intracorporeal robotic RAT was performed. An end-to-side anastomosis was performed between the renal vein and the external iliac vein and between the renal artery and the external iliac artery. Ureteroneocystostomy was also performed in 2 cases. All cases were performed robotically without open conversion. The median (IQR) console time was 3.1 (0.7) h, and the operative time was 3.8 (1.1) h. The estimated blood loss was 30 (0) ml. The warm ischemia time was 4.0 (0.2) min, and the cold ischemia time was 97 (17) min. Intracorporeal transarterial hypothermic renal perfusion was feasible in the 2 complete intracorporeal robotic RAT cases by using a perfusion catheter through a laparoscopic port. Robotic RAT has the potential to be a new minimally invasive substitute for conventional open surgery.

    DOI: 10.18926/AMO/57953

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  • Post-ablation syndrome after percutaneous cryoablation of small renal tumors: A prospective study of incidence, severity, duration, and effect on lifestyle

    Kawabata T, Hiraki T, Iguchi T, Matsui Y. Uka M, Masaoka Y, Komaki T, Sakurai J, Gobara H, Araki M, Nasu Y, Kanazawa S

    Eur J Radiol   2020年

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  • Comparison of longitudinal health‐related quality‐of‐life outcomes between anterior and posterior surgical approaches to robot‐assisted radical prostatectomy

    Maruyama Y, Sadahira T, Araki M, Wada K, Tanimoto R, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    J of Robotic Surgery   14 ( 2 )   255 - 260   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Prognostic role of the urokinase plasminogen activator (uPA) system in patients with nonmuscle invasive bladder cancer

    Iwata T, Kimura S, Abufarai M, Janisch F, Parizi M.K, Haitel A, Rink M, Roupret M, Fajkovic H, Seebacher V, Nyirady P, Karakiewicz P.I, Enikeev D, Rapoport L.M, Nasu Y, Shariat S.F

    Urol Oncol   37 ( 10 )   774 - 783   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • 腎移植後に発生し治療により完治したリンパ嚢腫の2例 査読

    窪田 理沙, 荒木 元朗, 和田 耕一郎, 河村 香澄, 丸山 雄樹, 光井 洋介, 西村 慎吾, 山下 里美, 小林 泰之, 渡邉 豊彦, 那須 保友, 加納 弓月, 北川 正史, 田邊 克幸, 和田 淳

    移植   54 ( 総会臨時 )   278 - 278   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • Upregulation of Mobility in Pancreatic Cancer Cells by Secreted S100A11 Through Activation of Surrounding Fibroblasts. 査読 国際誌

    Yosuke Mitsui, Nahoko Tomonobu, Masami Watanabe, Rie Kinoshita, I Wayan Sumardika, Chen Youyi, Hitoshi Murata, Ken-Ichi Yamamoto, Takuya Sadahira, Acosta Gonzalez Herik Rodrigo, Hitoshi Takamatsu, Kota Araki, Akira Yamauchi, Masahiro Yamamura, Hideyo Fujiwara, Yusuke Inoue, Junichiro Futami, Ken Saito, Hidekazu Iioka, Eisaku Kondo, Masahiro Nishibori, Shinichi Toyooka, Yasuhiko Yamamoto, Yasutomo Nasu, Masakiyo Sakaguchi

    Oncology research   27 ( 8 )   945 - 956   2019年8月

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    記述言語:英語  

    S100A11, a member of the S100 family of proteins, is actively secreted from pancreatic ductal adenocarcinoma (PDAC) cells. However, the role of the extracellular S100A11 in PDAC progression remains unclear. In the present study, we investigated the extracellular role of S100A11 in crosstalking between PDAC cells and surrounding fibroblasts in PDAC progression. An abundant S100A11 secreted from pancreatic cancer cells stimulated neighboring fibroblasts through receptor for advanced glycation end products (RAGE) upon S100A11 binding and was followed by not only an enhanced cancer cell motility in vitro but also an increased number of the PDAC-derived circulating tumor cells (CTCs) in vivo. Mechanistic investigation of RAGE downstream in fibroblasts revealed a novel contribution of a mitogen-activated protein kinase kinase kinase (MAPKKK), tumor progression locus 2 (TPL2), which is required for positive regulation of PDAC cell motility through induction of cyclooxygenase 2 (COX2) and its catalyzed production of prostaglandin E2 (PGE2), a strong chemoattractive fatty acid. The extracellularly released PGE2 from fibroblasts was required for the rise in cellular migration as well as infiltration of their adjacent PDAC cells in a coculture setting. Taken together, our data reveal a novel role of the secretory S100A11 in PDAC disseminative progression through activation of surrounding fibroblasts triggered by the S100A11-RAGE-TPL2-COX2 pathway. The findings of this study will contribute to the establishment of a novel therapeutic antidote to PDACs that are difficult to treat by regulating cancer-associated fibroblasts (CAFs) through targeting the identified pathway.

    DOI: 10.3727/096504019X15555408784978

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  • 当院におけるニボルマブ+イピリムマブの初期経験 査読

    片山 聡, 高本 篤, 関戸 崇了, 角南 亮輔, 佐久間 貴文, 和田里 章悟, 河村 香澄, 丸山 雄樹, 光井 洋介, 窪田 理沙, 大岩 裕子, 定平 卓也, 岩田 健宏, 西村 慎吾, 佐古 智子, 和田 耕一郎, 枝村 康平, 小林 泰之, 荒木 元朗, 石井 亜矢乃, 渡部 昌実, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   81 ( 4 )   483 - 484   2019年8月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • Oncologic outcomes after robot‑assisted versus open radical cystectomy: a systematic review and meta‑analysis

    Iwata T, Kimura S, Foerster B, Fossati N, Briganti A, Karakiewicz P.I, Gust K.M, Egawa S, Nasu Y, Abufaraj M, Shariat S.F

    World J Urol   37 ( 8 )   1557 - 1570   2019年8月

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    記述言語:英語  

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  • Comparison of the predictive value among inflammation-based scoring systems for bleomycin pulmonary toxicity in patients with germ cell tumors

    Maruyama Y, Sadahira T, Araki M, Mitsui Y, Wada K, Edamura K, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    Int J Urol   26 ( 8 )   813 - 819   2019年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Comparison of perioperative complications and health-related quality of life between robot-assisted and open radical cystectomy:A systematic review and meta-anasis

    Kimura S, Iwata T, Foerster B, Fossati N, Briganti A, Nasu Y, Egawa S, Abufaraj M, Shariat S.F

    Int J Urol   26 ( 8 )   760 - 774   2019年8月

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    記述言語:英語  

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  • Interobserver variability of 3.0-tesla and 1.5-tesla magnetic resonance imaging/computed tomography fusion image-based post -implant dosimetry of prostate brachytherapy

    Watanabe K, Katayama N, Katsui K, Matsushita T, Takamoto A, Ihara H, Nasu Y, Takemoto M, Kuroda M, Kanazawa S

    J Radiat Res   60 ( 4 )   483 - 489   2019年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Inflammatory myofibroblastic bladder tumor with divergent behavior in a patient with spinal cord injury

    Tsuboi I, Maruyama Y, Sadahira T, Wada K, Ando N, Mitsui Y, Nishimura Y, Arata R, Nasu Y, Ono N

    IJU   2 ( 4 )   212 - 214   2019年7月

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    記述言語:英語  

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  • The role of adjuvant radiotherapy after surgery for upper and lower urinary tract urothelialcarcinoma: A systematic review

    Iwata T, Kimura S, Abufaraj M, Janisch F, Karakiewicz P.I, Seebacher V, Roupre't M, Nasu Y, Shariat S.F

    Urol Oncol   37 ( 10 )   659 - 671   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Clinical phamacokinetics of oral azithromycin in epididymal tissue

    Sadahira T, Wada K, Ikawa K, Morikawa N, Mitsui M, Araki M, Fujiyoshi M, Ishii A, Watanabe M, Watanabe T, Nasu Y

    J Infect Chemother   25 ( 10 )   832 - 834   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Rituximab induction therapy is effective without increasing adverse events in immunological high risk recipients

    Kawamura K, Araki M, Maruyama Y, Mitsui Y, Sadahira T, Kubota T, Nishimura S, Wada K, Kobayashi Y, Watanabe T, Nasu Y

    The Journal of Urology   201(Supplement 4) ( 4S )   e1124 - e1125   2019年5月

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    記述言語:英語  

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  • Pelvic magnetic resonance imaging parameters predict urinary incontinence after robot-assisted radical prostatectomy

    Sadahira T, Mitsui Y, Araki M, Maruyama Y, Wada K, Edamura K, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    LUTS-Low Urin Tract Symptoms   11 ( 3 )   122 - 126   2019年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Perioperative blood transfusion affects oncologic outcomes after nephrectomy for renal cellcarcinoma: A systematic review and meta-analysis

    Iwata T, Kimura S, Foerster B, Abufaraj M, Karakiewicz P, Preisse F, Nasu Y, Shariat S.F

    Urol Oncol   37 ( 4 )   273 - 281   2019年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Contrast-enhanced computed tomography-guided percutaneous cryoablation of renal cell carcinoma in a renal allograft:First case in Asia

    Tsuboi I, Araki M, Fujiwara H, Iguchi T, Hiraki T, Arichi N, Kawamura K, Maruyama Y, Mitsui Y, Sadahira T, Kubota R, Nishimura S, Sako T, Takamoto A, Wada K, Kobayashi Y, Watanabe T, Yanai H, Kitagawa M, Tanabe K, Sugiyama H, Wada J, Shiina H, Kanazawa S, Nasu Y

    Acta Med. Okayama   73 ( 3 )   269 - 272   2019年4月

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    記述言語:英語  

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  • Use of single-dose perioperative antimicrobial therapy is acceptable in recipients of living-donor renal transplants in the rituximab era

    Nishimura S, Wada K, Araki M, Sadahira T, Maruyama Y, Mitsui Y, Tanimoto R, Kobayashi Y, Watanabe T, Nasu Y

    J infect Chemother   25 ( 4 )   247 - 252   2019年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Comparison of longitudinal health-related quality of life outcomes between the anterior and posterior surgical approach to robot-assisted radical prostatectomy.

    Maruyama Y, Sadahira T, Araki M, Tanimoto R, Kawamura K, Mitsui Y, Wada K, Edamura K, Kobayashi Y, Watanabe M, Nasu Y

    European Urology Supplements   18 ( 1 )   e1186 - e1187   2019年3月

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    記述言語:英語  

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  • Editorial comment from Dr.Sadahira et al. to use of fosfomycin as targeted antibiotic prophylaxis before prostate biopsy:a prospective randomized study

    Sadahira T, Wada K, Nasu Y

    Int J Urol   26 ( 3 )   399   2019年3月

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    記述言語:英語  

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  • Clinical impact of abdominal fat distribution measured by 3-D computed tomography volumetry on post-transplant renal function in recipients after living kidney transplantation:a retrospective study

    Mitsui Y, Sadahira T, Araki M, Maruyama Y, Wada K, Tanimoto R, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    Clin Exp Nephrol   23 ( 3 )   415 - 424   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • The 3-D volumetric measurement including resected specimen for predicting renal function afterRobot-assisted partial nephrectomy

    Mitsui Y, Sadahira T, Araki M, Maruyama Y, Nishimura S, Wada K, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    Urology   125   104 - 110   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Sarcopenia patients are clinically dissatisfied with postoperative urinary function compared with non-sarcopenia patients in robot-assisted radical prostatectomy

    Mitsui Y, Araki M, Maruyama Y, Sadahira T, Wada K, Edamura K, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    European Urology Supplements   18 ( 1 )   e1194 - e1195   2019年3月

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    記述言語:英語  

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  • The level of QOL improves slower in preemptive kidney transplantation than the one in non-preemptive kidney transplantation

    Mitsui Y, Araki M, Maruyama Y, Sadahira T, Wada K, Edamura K, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    European Urology Supplements   18 ( 1 )   e1651 - e1652   2019年3月

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  • Tumor suppressor REIC/Dkk-3 and its co-chaperone SGTA: Their interaction and role to control castration-resistant prostate cancer by the release from androgen independence and malignancy

    Sadahira T, Maruyama Y, Mitsui Y, Wada K, Edamura K, Kobayashi Y, Araki M, Watanabe M, Nasu Y

    European Urology Supplements   18 ( 1 )   e68   2019年3月

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  • Loss of psoas major muscle volume during systemic chemotherapy is related to worse prognosis in testicular cancer

    Mitsui Y, Sadahira T, Araki M, Maruyama Y, Wada K, Tanimoto R, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    Jpn J Clin Oncol   49 ( 2 )   183 - 189   2019年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Clitoral blood flow changes after surgery with tension-free vaginal mesh for pelvic organ prolapse

    Oiwa Y, Watanabe T, Sadahira T, Ishii A, Sako T, Inoue M, Wada K, Kobayashi Y, Araki M, Nasu Y

    Acta Med. Okayama   73 ( 1 )   21 - 27   2019年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • The Novel Combination of Nitroxoline and PD-1 Blockade, Exerts a Potent Antitumor Effect in a Mouse Model of Prostate Cancer. 査読 国際誌

    Naijin Xu, Linglong Huang, Xiezhao Li, Masami Watanabe, Chaoming Li, Abai Xu, Chunxiao Liu, Qiang Li, Motoo Araki, Koichiro Wada, Yasutomo Nasu, Peng Huang

    International journal of biological sciences   15 ( 5 )   919 - 928   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Programmed cell death protein 1 (PD-1) blockade is a promising therapeutic strategy against prostate cancer. Nitroxoline has been found to have effective anticancer properties in several cancer types. We investigated the efficacy of a combination therapy involving nitroxoline and PD-1 blockade in a prostate cancer mouse model. In our in vitro analysis, we found that nitroxoline inhibited the viability and proliferation of the mouse prostate cancer cell line RM9-Luc-PSA. Additionally, nitroxoline downregulated the expressions of phospho-PI3 kinase, phospho-Akt (Thr308), phospho-Akt (Ser473), phospho-GSK-3β, Bcl-2, and Bcl-xL. Nitroxoline also downregulated programmed death-ligand 1 (PD-L1) expression levels in prostate cancer cell line and tumor tissue. In our murine prostate cancer orthotopic model, nitroxoline plus PD-1 blockade synergistically suppressed tumor growth when compared with nitroxoline or PD-1 blockade alone, leading to reductions in tumor weight, bioluminescence tumor signals, and serum prostate-specific antigen levels. Furthermore, fluorescence-activated cell sorting analysis showed that the combination strategy significantly enhanced antitumor immunity by increasing CD44+CD62L+CD8+ memory T cell numbers and reducing myeloid-derived suppressor cell numbers in peripheral blood. In conclusion, our findings suggest that nitroxoline plus PD-1 blockade may be a promising treatment strategy in patients with prostate cancer.

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  • Favorable long-term oncological and urinary outcomes of incidental prostate cancer following holmium laser enucleation of the prostate

    Tominaga Y, Sadahira T, Mitsui Y, Maruyama Y, Tanimoto R, Wada K, Munemasa S, Kusaka N, Nishiyama Y, Kurashige T, Nasu Y, Hayata S

    Mol Clin Oncol   10 ( 6 )   605 - 609   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Burned-out Prostate Cancer ? Primary Metastatic Cancer Not Detected on Repeat Biopsy. 査読

    Mitsui Y, Sadahira T, Maruyama Y, Wada K, Tanimoto R, Sugimoto M, Araki M, Watanabe M, Yanai H, Watanabe T, Nasu Y

    Acta medica Okayama   72 ( 6 )   605 - 609   2018年12月

  • Prospective randomized controlled trial of postoperative early intravesical chemotherapy with pirarubicin (THP) for solitary non-muscle invasive bladder cancer comparing single and two-time instillation 査読

    Ryuta Tanimoto, Takashi Saika, Shin Ebara, Yasuyuki Kobayashi, Ryoji Nasu, Daisuke Yamada, Hitoshi Takamoto, Yoshiyuki Miyaji, Yasutomo Nasu, Tomoyasu Tsushima, Hiromi Kumon

    World Journal of Urology   36 ( 6 )   889 - 895   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Verlag  

    Purpose: Single immediate intravesical instillation of chemotherapy after transurethral resection of bladder tumor (TURBT) has been the gold standard treatment for patients with low- and intermediate-risk non-muscle invasive bladder cancer (NMIBC). Herein, we conducted a multicenter prospective randomized controlled trial in Japan, comparing recurrence-free survival between single and two-time instillation of pirarubicin (THP) for solitary NMIBC. Methods: Between 2005 and 2009, 257 patients with solitary NMIBC were enrolled and randomized to single instillation of THP (30 mg/50 mL) immediately after TURBT (Group A) or two-time instillation of THP immediately after and 1 day after TURBT (Group B). The primary endpoint was recurrence-free survival. Secondary endpoints included rates of recurrence and adverse effects, including hematuria, micturition pain, difficult urination, pollakiuria, systemic symptoms, and other complications. This study was registered as UMIN C000000266. Results: Of 257 patients, 99 in Group A and 102 in Group B could be evaluated for recurrence. Median follow-up was 71 months. The overall recurrence rate was 39 and 31%, respectively (p = 0.2704). Although the 5-year recurrence-free survival rates were 55.9% and 67.7% in groups A and B, respectively, the difference between groups was not significant (p = 0.2031). No significant differences in adverse effects were observed between groups, except for pollakiuria (7 vs 22%, p = 0.0031). Multivariate analyses did not show that the treatment group was a significant risk factor for bladder cancer recurrence. Conclusions: Postoperative two-time intravesical instillation of THP was not superior to single immediate instillation for preventing recurrence after complete resection of a solitary NMIBC.

    DOI: 10.1007/s00345-018-2196-8

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  • Renal function after nephrectomy influences the risk of cardiovascular events

    Kambara T, Tanimoto R, Araki M, Saika T, Hashimoto H, Oeda T, Tsushima T, Hayata S, Nasu Y, Kobayashi Y

    Acta Med. Okayama   72 ( 3 )   241 - 247   2018年6月

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    記述言語:英語   掲載種別:研究論文(大学,研究機関等紀要)  

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  • Prognostic impact of bleomycin pulmonary toxicity on the outcomes of patients with testicular cancer

    Maruyama Y, Sadahira T, Mitsui Y, Takamoto A, Wada K, Tanimoto R, Kobayashi Y, Araki M, Watanabe M, Watanabe T, Nasu Y

    The Journal of Urology   199 ( 4S )   e1131 - e1132   2018年5月

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  • Oncological impact of neoadjuvant hormonal therapy on permanentiodine-125 seed brachytherapy in patients with low- and intermediate-risk prostate cancer

    Takamoto A, Tanimoto R, Bekku K, Araki M, Sadahira T, Wada K, Ebara S, Katayama N, Yanai H, Nasu Y

    Int J Urol   25 ( 5 )   507 - 512   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Clinical impact of abdominal fat distribution using 3-D computed tomography volumetry on renal function after renal transplantation

    Mitsui Y, Sadahira T, Maruyama Y, Takamoto A, Wada K, Tanimoto R, Kobayashi Y, Araki M, Watanabe M, Watanabe T, Nasu Y

    The Journal of Urology   199 ( 4S )   e831   2018年4月

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  • Prognostic impact of bleomycin pulmonary toxicity on the outcomes of patients with germ cell tumors

    Maruyama Y, Sadahira T, Mitsui Y, Araki M, Wada K, Tanimoto R, Kobayashi Y, Watanabe M, Watanabe T, Nasu Y

    Med Oncol   35 ( 6 )   453 - 458   2018年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Acute Inflammatory Syndrome Paradoxically Induced by de novo Purine Inhibitors Synthesis before Renal Transplantation: A Case Report and Review of the Literature

    Maruyama Y, Sadahira T, Mitsui Y, Wada K, Tanimoto R, Nishimura S, Kobayashi Y, Watanabe T, Nasu Y, Araki M

    Transplant Proc   50 ( 3 )   895 - 897   2018年4月

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  • Phase Ⅱstudy of radium-223 dichloride in Japanese patients with symptomatic castration -resistant prostate cancer

    Matsubara N, Ngamori S, Wakumoto Y, Uemura H, Kimura G, Yokomizo A, Kikukawa H, Mizokami A, Kosaka T, Masumori N, Kawasaki Y, Yonese J, Nasu Y, Fukasawa S, Sugiyama T, Kimura S, Hosono M, Yamaguchi I, Tsutsui H, Uemura H

    Int J Clin Oncol   23 ( 1 )   173 - 180   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Induction of cells with prostate cancer stem-like properties from mouse induced pluripotent stem cells via conditioned medium. 査読 国際誌

    Naijin Xu, Xiezhao Li, Masami Watanabe, Hideo Ueki, Hao Hu, Na Li, Motoo Araki, Koichiro Wada, Abai Xu, Chunxiao Liu, Yasutomo Nasu, Peng Huang

    American journal of cancer research   8 ( 8 )   1624 - 1632   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cancer stem cells (CSCs) that closely correlated with tumor growth, metastasis, provide a plausible explanation for chemoresistance and cancer relapse. CSCs are usually isolated and enriched from carcinoma cells, which is inconvenient, low-efficient, and even unreliable. Here, we converted mouse induced pluripotent stem cells (miPSCs) into prostate cancer stem-like cells with carcinoma microenvironment following exposure to conditioned medium (CM) derived from RM9, a mouse prostate cancer cell line. These transformed cells, termed as miPS-RM9CM, displayed CSCs properties, including spheroids morphology and expression of both stemness genes and cancer stem cells surface markers, such as Oct3/4, Sox2, Nanog, Klf-4, c-Myc, CD44, and CD133. In addition, in vivo transplantation experiment was performed to confirm the tumorigenicity. Furthermore, we used the model to assess conventional chemotherapeutic agent, docetaxel. The results showed that miPS-RM9CM cells exhibited increased resistance to docetaxel, however, high susceptibility to the cancer cell stemness inhibitor I (BBI-608). Our current study demonstrates that CM from cultured RM9 cells play a crucial role in the determination of cell fate from miPSCs to cancer stem-like cells and provide a potentially valuable system for the study of CSCs.

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  • Sarcopenia in patients with testicular cancer undergoing chemotherapy :Prognostic impact of psoas major muscle loss

    Mitsui Y, Sadahira T, Maruyama Y, Takanoto A, Wada K, Tanimoto R, Kobayashi Y, Araki M, Watanabe M, Watanabe T, Nasu Y

    European Urology Supplements   17 ( 2 )   e1137 - e1138   2018年

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  • Red nodular melanoma of the penile foreskin : A case report and literature review

    Maruyama Y, Sadahira T, Mitsui Y, Wada K, Tanimoto R, Kobayashi Y, Araki M, Watanabe M, Watanabe T, Nasu Y

    Mol Clin Oncol   9 ( 4 )   449 - 452   2018年

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  • Impact of selective media for detecting fluoroquinolone-insusceptible/extended-spectrum beta-lactamase-producing Escherichia coli before transrectal prostate biopsy 査読

    Takuya Sadahira, Koichiro Wada, Motoo Araki, Ayano Ishii, Toyohiko Watanabe, Yoshitsugu Nasu, Masaya Tsugawa, Tadasu Takenaka, Yasutomo Nasu, Hiromi Kumon

    INTERNATIONAL JOURNAL OF UROLOGY   24 ( 12 )   842 - 847   2017年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    ObjectivesTo investigate the prevalence of fluoroquinolone-insusceptible and/or extended-spectrum beta-lactamase-producing Escherichia coli colonizing in the male rectum before transrectal prostate biopsy.
    MethodsWe carried out a prospective cohort study of men undergoing transrectal prostate biopsy. CHROMagar Orientation originally supplemented with levofloxacin and CHROMagar Orientation/extended-spectrum beta-lactamase were used for detecting fluoroquinolone-insusceptible and extended-spectrum beta-lactamase-producing Escherichia coli. Rectal specimens were collected before prostate biopsy, and the results of cultures in the selective medium were compared with drug susceptibility measured by standard methods. Targeted prophylactic antimicrobials were administered to patients with drug-resistant Escherichia coli and the incidence of postoperative prostatitis was investigated. In the case of prostatitis, pathogens preoperatively isolated from the rectum and those from urine were compared using pulsed-field gel electrophoresis.
    ResultsRectal colonization of fluoroquinolone-insusceptible or extended-spectrum beta-lactamase-producing Escherichia coli was detected in 217 of 694 (31.3%) and 85 of 640 (13.3%) participants, respectively. The sensitivity and specificity of fluoroquinolone-insusceptible selective media were 96.8% and 88.2%, respectively. A total of 618 participants underwent transrectal prostate biopsy, and postoperative acute prostatitis was observed in four of 618 (0.6%) participants. Escherichia coli strains isolated preoperatively from the rectum and postoperatively from urine were found to be identical.
    ConclusionsThe present findings showed accuracy and performance of the selective media. Screening cultures before transrectal prostate biopsy using selective media seems to be helpful for guiding antibiotic prophylaxis and thus decreasing the rate of post-biopsy acute prostatitis.

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  • Exogenous DKK-3/REIC inhibits Wnt/beta-catenin signaling and cell proliferation in human kidney cancer KPK1 査読

    Jiaqi Xu, Takuya Sadahira, Rie Kinoshita, Shun-Ai Li, Peng Huang, Koichiro Wada, Motoo Araki, Kazuhiko Ochiai, Hirofumi Noguchi, Masakiyo Sakaguchi, Yasutomo Nasu, Masami Watanabe

    ONCOLOGY LETTERS   14 ( 5 )   5638 - 5642   2017年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    The third member of the Dickkopf family (DKK-3), also known as reduced expression in immortalized cells (REIC), is a tumor suppressor present in a variety of tumor cells. Regarding the regulation of the Wnt/beta-catenin signaling pathway, exogenous DKK-1 and DKK-2 are reported to inhibit Wnt signaling by binding the associated effectors. However, whether exogenous DKK-3 inhibits Wnt signaling remains unclear. A recombinant protein of human full-length DKK-3 was used to investigate the exogenous effects of the protein in vitro in KPK1 human renal cell carcinoma cells. It was demonstrated that the expression of phosphorylated (p-)beta-catenin (inactive form as the transcriptional factor) was increased in KPK1 cells treated with the exogenous DKK-3 protein. The levels of non-p-beta-catenin (activated form of beta- catenin) were consistently decreased. It was revealed that the expression of transcription factor (TCF) 1 and c-Myc, the downstream transcription factors of the Wnt/beta-catenin signaling pathway, was inhibited following treatment with DKK-3. A cancer cell viability assay confirmed the anti-proliferative effects of exogenous DKK-3 protein, which was consistent with a suppressed Wnt/beta-catenin signaling cascade. In addition, as low-density lipoprotein receptor-related protein 6 (LRP6) is a receptor of DKK-1 and DKK-2 and their interaction on the cell surface inhibits Wnt/beta-catenin signaling, it was examined whether the exogenous DKK-3 protein affects LRP6-mediated Wnt/beta-catenin signaling. The LRP6 gene was silenced and the effects of DKK-3 on the time course of the upregulation of p-beta-catenin expression were subsequently analyzed. Notably, LRP6 depletion elevated the base level of p-beta-catenin; however, there was no significant effect on its upregulation course or expression pattern. These findings indicate that exogenous DKK-3 upregulates p-beta-catenin and inhibits Wnt/beta-catenin signaling in an LRP6-independent manner. Therefore, exogenous DKK-3 protein may inhibit the proliferation of KPK1 cells via inactivating Wnt/beta-catenin signaling.

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  • Ureterolithotripsy for a Ureteral Calculus at the Ureteroureterostomy of a Renal-transplant Recipient 査読

    Yosuke Mitsui, Koichiro Wada, Motoo Araki, Takashi Yoshioka, Yuichi Ariyoshi, Shingo Nishimura, Yasuyuki Kobayashi, Katsumi Sasaki, Toyohiko Watanabe, Yasutomo Nasu

    ACTA MEDICA OKAYAMA   71 ( 5 )   449 - 452   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We describe a 40-year-old living-donor renal-transplant recipient who underwent successful ureterolithotripsy. He had been on hemodialysis for >15 years pre-transplant and underwent ureteroureterostomy along with the surgery. One year post-transplant, ultrasound examination demonstrated hydronephrosis, and CT showed a 6-mm ureteral calculus at the ureteroureterostomy site. No pain and no elevated serum creatinine were present. As the ureter was easily accessed, we performed a ureterolithotripsy, which would confirm whether a suture caused the calculus. Despite ureteral tortuosity, laser stone fragmentation succeeded. The calculus was completely removed with an antegrade guidewire. Mild postoperative ureteral stenosis resolved with a temporary ureteral stent without balloon dilation. Ureterolithotripsy is effective even in renal transplant recipients with ureteroureterostomy.

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  • Promising therapeutic efficacy of a novel reduced expression in immortalized cells/dickkopf-3 expressing adenoviral vector for hepatocellular carcinoma 査読

    Hiroaki Sawahara, Hidenori Shiraha, Daisuke Uchida, Hironari Kato, Ryo Kato, Atsushi Oyama, Teruya Nagahara, Masaya Iwamuro, Shigeru Horiguchi, Koichiro Tsutsumi, Mari Mandai, Tetsushige Mimura, Nozomu Wada, Yasuto Takeuchi, Kenji Kuwaki, Hideki Onishi, Shinichiro Nakamura, Masami Watanabe, Masakiyo Sakaguchi, Akinobu Takaki, Kazuhiro Nouso, Takahito Yagi, Yasutomo Nasu, Hiromi Kumon, Hiroyuki Okada

    JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY   32 ( 10 )   1769 - 1777   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Background and Aim: Reduced expression in immortalized cells (REIC)/dickkopf-3 (Dkk-3) is a tumor suppressor gene that is downregulated in various cancers. In our previous study of prostate cancer, the REIC/Dkk-3-expressing adenoviral vector (Ad-REIC) was found to induce cancer-selective apoptosis. This study recently developed a novel super gene expression (SGE) system and used this system to re-construct an Ad-REIC vector, termed the Ad-SGE-REIC, to achieve more effective therapeutic outcomes. In this study, the therapeutic effects of Ad-SGE-REIC on hepatocellular carcinoma (HCC) was assessed.
    Methods: Human HCC cell lines (HLE, Huh7, HepG2, HLF, SK-Hep1, and PLC), human HCC tissues, and mouse HCC cell line (Hepa1-6) were used in this study. REIC/Dkk-3 expression was assessed by immunoblotting and immunohistochemistry. The relative cell viability and the apoptotic effect were examined in vitro, and the anti-tumor effects of Ad-SGE-REIC treatment were analyzed in the mouse xenograft model. This study additionally assessed anti-tumor immunological effects on the immunocompetent mice.
    Results: REIC/Dkk-3 expression was decreased in HCC cell lines and HCC tissues. Ad-SGE-REIC reduced cell viability and induced apoptosis in HCC cell lines (HLE and Huh7), inhibited tumor growth in the mouse xenograft model, and demonstrated in vivo anti-cancer immunostimulatory effects on the HCC cell line (Hepa1-6).
    Conclusions: Ad-SGE-REIC treatment not only enhanced cell killing effects in vitro but also elicited significant therapeutic effects, with tumor growth suppression, in vivo. REIC/Dkk-3 gene therapy using Ad-SGE-REIC potentially represents an innovative new therapeutic tool for HCC.

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  • Overexpression of REIC/Dkk-3 suppresses the expression of CD147 and inhibits the proliferation of human bladder cancer cells 査読

    Yuhei Horikawa, Masami Watanabe, Takuya Sadahirai, Yuichi Ariyoshi, Yasuyuki Kobayashi, Motoo Araki, Koichiro Wada, Kazuhiko Ochiai, Shun-Ai Li, Yasutomo Nasu

    ONCOLOGY LETTERS   14 ( 3 )   3223 - 3228   2017年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    Our group previously developed an adenoviral vector encoding the REIC/Dkk-3 gene (Ad-REIC), a tumor suppressor, for cancer gene therapy. The Ad-REIC agent induces apoptosis and inhibits invasion in a number of cancer cell lines; however, the molecular mechanisms underlying its effects remain unclear. Cluster of differentiation 147 (CD147), also known as extracellular matrix metalloproteinase inducer (EMMPRIN), is a key molecule that promotes cancer proliferation and invasion. In order to elucidate the therapeutic mechanism of Ad-REIC, its effect on the expression of CD147 in human bladder cancer KK47 cells was investigated. Treatment with Ad-REIC markedly downregulated the expression of CD147 and significantly inhibited cellular proliferation. Since the expression of CD147 is reported to be under the positive control of mitogen-activated protein kinase (MAPK) signaling and the c-Myc protein, the correlations between the expression of CD147 and the activation of MAPKs or the expression of c-Myc were examined. Unexpectedly, no positive correlation was observed between the level of CD147 and the potential regulators that were assessed, indicating that another signaling pathway is responsible for the downregulation of CD147. The results from the present study demonstrate that Ad-REIC treatment can significantly downregulate the expression of CD147 in bladder cancer cells. Downregulation of the cancer-progression factor CD147 may be a novel mechanism that underlies the therapeutic effects of Ad-REIC treatment.

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  • Robotic Renal Autotransplantation: First Case Outside of North America 査読

    Motoo Araki, Koichiro Wada, Yosuke Mitsui, Takuya Sadahira, Risa Kubota, Shingo Nishimura, Takashi Yoshioka, Yuichi Ariyoshi, Kei Fujio, Atsushi Takamoto, Morito Sugimoto, Katsumi Sasaki, Yasuyuki Kobayashi, Shin Ebara, Hideki Taninishi, Hiroyuki Amano, Masashi Inui, Masami Watanabe, Toyohiko Watanabe, Yasutomo Nasu

    ACTA MEDICA OKAYAMA   71 ( 4 )   351 - 355   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    A 38-year-old woman with a 2.7-cm left ureteral stenosis requiring chronic ureteral stent exchange elected to undergo robotic renal autotransplantation. Left ureteropelvic junction obstruction (UPJO) was also suspected. Robotic donor nephrectomy contributed to the fine dissection for desmoplastic changes. The kidney was removed through a Gelport and examined on ice. UPJO was not seen. An end-to-side robotic anastomosis was created between the renal and external iliac vessels. The console time was 507 min, and the warm ischemia time was 4 min 5 sec. She became stent-free. Robotic renal autotransplantation is a new, minimally invasive approach to renal preservation.

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  • Antimicrobial susceptibilities of Chlamydia trachomatis isolated from the urethra and pharynx of Japanese males 査読

    Seiji Kai, Koichiro Wada, Takuya Sadahira, Motoo Araki, Ayano Ishii, Toyohiko Watanabe, Koichi Monden, Satoshi Uno, Tohru Araki, Yasutomo Nasu

    JOURNAL OF INFECTION AND CHEMOTHERAPY   23 ( 8 )   512 - 516   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Objectives: Sexually transmitted infections due to Chlamydia trachomatis (C. trachomatis) are a worldwide public health problem. The aim of this study was to investigate the drug susceptibilities of C. trachomatis strains isolated from the urethra and pharynx of Japanese males.
    Methods: Urethral and pharyngeal swabs were collected between 2013 and 2014 from Japanese males with urethritis. Using a McCoy cell line, 18 chlamydial strains were isolated from urethra in 18 patients and 7 from the pharynx in 7 of the 18 patients. The minimum inhibitory concentrations (MICs) of levofloxacin (LVFX) and azithromycin (AZM) were measured using the standard method of the Japanese Society of Chemotherapy.
    Results: The MICs of LVFX and AZM against urethral chlamydial strains were 0.125-0.5 mu g/mL and 0.125-0.25 mu g/mL, respectively. In pharyngeal strains, the MICs of LVFX and AZM were 0.125-0.25 mu g/mL and 0.125-0.25 mu g/mL, respectively. In 7 patients with chlamydial strains isolated from both the urethra and pharynx, the MICs of LVFX between these strains were identical in 3 of 6 patients (no growth was observed for one pharyngeal strain), while the MICs of AZM between these strains were identical in all 6 patients (not performed for one patient).
    Conclusions: Our data suggest that C. trachomatis strains isolated from the urethra and pharynx of Japanese males are susceptible to LVFX and AZM. Although measuring the MICs of chlamydial strains is labor intensive, it is a significant surveillance tool for treating chlamydial infections and preventing the spread of STIs. (C) 2017 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

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  • Robust cancer-specific gene expression by a novel cassette with hTERT and CMV promoter elements 査読

    Masakiyo Sakaguchi, Takuya Sadahira, Hideo Ueki, Rie Kinoshita, Hitoshi Murata, Ken-Ichi Yamamoto, Junichiro Futami, Yasutomo Nasu, Kazuhiko Ochiai, Hiromi Kumon, Nam-Ho Huh, Masami Watanabe

    ONCOLOGY REPORTS   38 ( 2 )   1108 - 1114   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    We developed and validated a novel hTERT/CMV promoter element-driven gene expression cassette that can robustly enhance cancer-specific gene expression. The following gene expressional elements were located in tandem within the plasmid construct: [hTERT core promoter, cytomegalovirus (CMV) minimized promoter, RU5' sequence, an inserted gene, BGH polyA, hTERT enhancer]; this is hereafter referred to as the hT/Cm-R-hT construct. Using various human cancer cell lines and normal cells, the cancer-specific transcription of the green fluorescent protein (GFP) gene was examined by western blotting and fluorescence microscopy. Cancer-specific gene expression was robustly achieved in the hT/Cm-R-hT plasmid in comparison to the other control hT/Cm-driven construct. Notably, the expression level of GFP observed in the hT/Cm-RhT-driven construct was superior to that of the control plasmid with the conventional CMV promoter in HEK293 cells, which are known to possess higher hTERT activity than normal cells. We next examined the availability of hT/Cm-R-hT in detecting the target GFP expressing cancer cells from human peripheral blood mononuclear cells (PBMCs). The hT/Cm-R-hT plasmid successfully induced cancer-specific gene expression; the robust expression of GFP was observed in target HeLa cancer cells, whereas GFP was not visibly expressed in normal PBMCs. The plasmid allowed for the selective visualization of viable HeLa cancer cells in mixed cell cultures containing up to 10000-fold more PBMCs. These findings indicate that the hT/Cm-R-hT expressional system is a valuable tool for detecting viable cancer cells mixed with normal cells. The current system can therefore be applied to the in vitro detection of cancer cells that are disseminated in the blood and other types of body fluid in vivo. Since the current system can also be applied to other types of vectors, including virus vectors, this approach using the hTERT promoter-based construct is expected to become a valuable tool for enhancing cancer specific gene expression.

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  • Expression of tumor suppressor REIC/Dkk-3 by a newly improved adenovirus vector with insertion of a hTERT promoter at the 3'-side of the transgene 査読

    Endy Widya Putranto, Rie Kinoshita, Masami Watanabe, Takuya Sadahira, Hitoshi Murata, Ken-Ichi Yamamoto, Junichiro Futami, Ken Kataoka, Yusuke Inoue, I. Made Winarsa Ruma, I. Wayan Sumardika, Chen Youyi, Miyoko Kubo, Yoshihiko Sakaguchi, Kenji Saito, Yasutomo Nasu, Hiromi Kumon, Nam-Ho Huh, Masakiyo Sakaguchi

    ONCOLOGY LETTERS   14 ( 1 )   1041 - 1048   2017年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    Reduced expression in immortalized cells (REIC)/Dickkopf-3 (Dkk-3) overexpression, induced using an adenovirus (Ad)-REIC, has been revealed to have a dramatic therapeutic effect on multiple types of cancer. To achieve an improved therapeutic effect from Ad-REIC on cancer, our group previously developed an enhanced gene expression system, the C-TSC cassette [cytomegalovirus (CMV)-RU5' located upstream (C); another promoter unit composed of triple tandem promoters, human telomerase reverse transcriptase (hTERT), simian virus 40 and CMV, located downstream of the cDNA (TSC); plus a polyadenylation (polyA) signal]. When applied to the conventional Ad-REIC, this novel system induced the development of an enhanced product, Ad-C-TSC-REIC, which exhibited a noticeable anticancer effect. However, there were difficulties in terms of Ad-C-TSC-REIC productivity in HEK293 cells, which are a widely used donor cell line for viral production. Productivity of Ad-C-TSC-REIC was significantly reduced compared with the conventional Ad-REIC, as the Ad-C-TSC-REIC had a significantly higher ability to induce apoptotic cell death of not only various types of cancer cell, but also HEK293 cells. The present study aimed to overcome this problem by modifying the C-TSC structure, resulting in an improved candidate: A C-T cassette (C: CMV-RU5' located upstream; T: another promoter unit composed of a single hTERT promoter, located downstream of the cDNA plus a polyA signal), which demonstrated gene expression comparable to that of the C-TSC system. The improved adenovirus REIC/Dkk-3 product with the C-T cassette, named Ad-C-T-REIC, exhibited a higher expression level of REIC/Dkk3, similar to that of Ad-C-TSC-REIC. Notably, the vector mitigated the cell death of donor HEK293 cells, resulting in a higher rate of production of its adenovirus. These results indicated that Ad-C-T-REIC has the potential to be a useful tool for application in cancer gene therapy.

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  • Successful microscopic renal autotransplantation for left renal aneurysm associated with segmental arterial mediolysis

    Yoshioka T, Araki M, Ariyoshi Y, Wada K, Tanaka N, Nasu Y

    Journal of Vascular Surgery   66 ( 1 )   261 - 264   2017年7月

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  • The Downregulation of the Expression of CD147 by Tumor Suppressor REIC/Dkk-3, and Its Implication in Human Prostate Cancer Cell Growth Inhibition 査読

    Akihiro Mori, Masami Watanabe, Takuya Sadahira, Yasuyuki Kobayashi, Yuichi Ariyoshi, Hideo Ueki, Koichiro Wada, Kazuhiko Ochiai, Shun-Ai Li, Yasutomo Nasu

    ACTA MEDICA OKAYAMA   71 ( 2 )   135 - 142   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    The cluster of differentiation 147 (CD147), also known as EMMPRIN, is a key molecule that promotes cancer progression. We previously developed an adenoviral vector encoding a tumor suppressor REIC/Dkk-3 gene (Ad-REIC) for cancer gene therapy. The therapeutic effects are based on suppressing the growth of cancer cells, but, the underlying molecular mechanism has not been fully clarified. To elucidate this mechanism, we investigated the effects of Ad-REIC on the expression of CD147 in LNCaP prostate cancer cells. Western blotting revealed that the expression of CD147 was significantly suppressed by Ad-REIC. Ad-REIC also suppressed the cell growth of LNCaP cells. Since other researchers have demonstrated that phosphorylated mitogen-activated protein kinases (MAPKs) and c-Myc protein positively regulate the expression of CD147, we investigated the correlation between the CD147 level and the activation of MAPK and c-Myc expression. Unexpectedly, no positive correlation was observed between CD147 and its possible regulators, suggesting that another signaling pathway was involved in the downregulation of CD147. This is the first study to show the downregulation of CD147 by Ad-REIC in prostate cancer cells. At least some of the therapeutic effects of Ad-REIC may be due to the downregulation of the cancer-progression factor, CD147.

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  • A Combination Therapy of Partial Nephrectomy and Cryoablation Achieved Good Cancer Control and Renal Function in Bilateral Synchronous Renal Cell Carcinoma 査読

    Atsushi Takamoto, Motoo Araki, Koichiro Wada, Morito Sugimoto, Yasuyuki Kobayashi, Katsumi Sasaki, Toyohiko Watanabe, Yasutomo Nasu

    ACTA MEDICA OKAYAMA   71 ( 2 )   187 - 190   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We report the case of a 58-year-old Japanese man with bilateral synchronous renal cell carcinoma (RCC). The diameters of the right and left tumors were 56 and 69 mm, respectively. Both tumors were endophytic. Cryoablation with prophylactic embolization was performed for the left tumor, and 1 month later, a right open partial nephrectomy was performed. No recurrence was observed during a 16-month follow-up, and the serum creatinine level has been stable. The prognosis of bilateral synchronous RCC is better than that of dialysis patients. The novel approach of combining cryoablation and partial nephrectomy can achieve good cancer control and renal function in bilateral RCC.

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  • Clinical pharmacokinetics of oral levofloxacin and sitafloxacin in epididymal tissue 査読

    Takuya Sadahira, Koichiro Wada, Kazuro Ikawa, Norifumi Morikawa, Hiroaki Kurahashi, Takashi Yoshioka, Yuichi Ariyoshi, Yasuyuki Kobayashi, Motoo Araki, Ayano Ishii, Masami Watanabe, Shinya Uehara, Toyohiko Watanabe, Yasutomo Nasu

    JOURNAL OF INFECTION AND CHEMOTHERAPY   23 ( 4 )   214 - 217   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Objectives: This study aimed to investigate the penetration of fluoroquinolones into human epididymal tissue.
    Methods: The penetration of levofloxacin (LVFX) 500 mg or sitafloxacin (STFX) 100 mg into epididymal tissue was examined. Patients with prostate cancer who were referred for orchiectomy were included. LVFX 500 mg (n = 9) or STFX 100 mg (n = 9) was administered orally 1 h before orchiectomy, and 0.5 g of epididymal tissue and blood samples were collected simultaneously during surgery. Drug concentrations were measured by high-performance liquid chromatography, and patient characteristics and adverse events were analyzed.
    Results: The mean ratio of the epididymal concentration to the serum concentration was 1.48 +/- 0.45 for LVFX and 1.54 +/- 0.81 for STFX. For LVFX, the simulated curves estimated the following: maximum concentrations (Cmax) of 8.84 mu g/ml in serum and 14.1 mu g/g in epididymal tissue and area under the concentration-time curve for 24 h (AUC(24)) of 68.5 mu g h/ml in serum and 108.9 mu g h/g in epididymal tissue. For STFX, the Cmax was 1.22 mu g/ml in serum and 1.66 mu g/g in epididymal tissue, and the AUC24 was 9.58 mu g h/ml in serum and 13.1 mu g h/g in epididymal tissue. Neither treatment-related adverse events nor postoperative urogenital infections were observed.
    Conclusions: The results of this study suggest that oral administration of LVFX 500 mg or STFX 100 mg achieves effective epididymal concentrations for treatment of epididymitis. (C) 2016 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jiac.2016.12.010

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  • Evaluation of urinary stone composition and differentiation between urinary stones and phleboliths using single-source dual-energy computed tomography

    Ogawa N, Sato S, Ida K, Kato K, Ariyoshi Y, Wada K, Nasu Y, Kanazawa S

    Acta Med. Okayama   71 ( 2 )   91 - 96   2017年4月

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    記述言語:英語   掲載種別:研究論文(大学,研究機関等紀要)  

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  • Synergistic effects of the immune checkpoint inhibitor CTLA-4 combined with the growth inhibitor lycorine in a mouse model of renal cell carcinoma 査読

    Xiezhao Li, Peng Xu, Chongshan Wang, Naijin Xu, Abai Xu, Yawen Xu, Takuya Sadahira, Motoo Araki, Koichiro Wada, Eiji Matsuura, Masami Watanabe, Junxia Zheng, Pinghua Sun, Peng Huang, Yasutomo Nasu, Chunxiao Liu

    ONCOTARGET   8 ( 13 )   21177 - 21186   2017年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IMPACT JOURNALS LLC  

    Renal cell carcinoma (RCC) management has undergone a major transformation over the past decade; immune checkpoint inhibitors are currently undergoing clinical trials and show promising results. However, the effectiveness of immune checkpoint inhibitors in patients with metastatic RCC (mRCC) is still limited. Lycorine, an alkaloid extracted from plants of the Amaryllidaceae family, is touted as a potential anti-cancer drug because of its demonstrative growth inhibition capacity (induction of cell cycle arrest and inhibition of vasculogenic mimicry formation). Moreover, T cell checkpoint blockade therapy with antibodies targeting cytotoxic T-lymphocyte associated protein 4 (CTLA-4) has improved outcomes in cancer patients. However, the anti-tumor efficacy of combined lycorine and anti-CTLA-4 therapy remains unknown. Thus, we investigated a combination therapy of lycorine hydrochloride and anti-CTLA-4 using a murine RCC model. As a means of in vitro confirmation, we found that lycorine hydrochloride inhibited the viability of various RCC cell lines. Furthermore, luciferase-expressing Renca cells were implanted in the left kidney and the lung of BALB/c mice to develop a RCC metastatic mouse model. Lycorine hydrochloride and anti-CTLA-4 synergistically decreased tumor weight, lung metastasis, and luciferin-staining in tumor images. Importantly, the observed anti-tumor effects of this combination were dependent on significantly suppressing regulatory T cells while upregulating effector T cells; a decrease in regulatory T cells by 31.43% but an increase in effector T cells by 31.59% were observed in the combination group compared with those in the control group). We suggest that a combination of lycorine hydrochloride and anti-CTLA-4 is a viable therapeutic option for RCC patients.

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  • Reality of nerve sparing and surgical margins in surgeons' early experience with robot-assisted radical prostatectomy in Japan

    Tatsugami K, Yoshioka K, Shiroki R, Eto M, Yoshino Y, Tozawa K, Fukasawa S, Fujisawa M, Takenaka A, Nasu Y, Kashiwagi A, Gotoh M, Terachi T

    Int J Urol   24 ( 3 )   191 - 196   2017年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Interfractional Seminal Vesicle Motion Relative to the Prostate Gland for Image-guided Radiotherapy for Prostate Cancer with/without Androgen Deprivation Therapy: A Retrospective Cohort Study 査読

    Takahiro Waki, Kuniaki Katsui, Toshiharu Mitsuhashi, Takeshi Ogata, Norihisa Katayama, Mitsuhiro Takemoto, Yasutomo Nasu, Hiromi Kumon, Susumu Kanazawa

    ACTA MEDICA OKAYAMA   71 ( 1 )   31 - 39   2017年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We investigated differences in seminal vesicle (SV) length and interfractional SV motion relative to the prostate gland in prostate cancer patients. We compared 32 patients who received androgen deprivation therapy (ADT) before radiotherapy with 12 patients receiving radiotherapy alone at Okayama University Hospital in August 2008-July 2011. We examined the right and left SVs' length and motion by computed tomography (CT) to determine the ADT's effects and analyzed 347 CT scans in a multiple linear regression model. The ADT patients' SV length was significantly shorter than the non-ADT patients'. The differences in right and left SV lengths between the ADT and non-ADT patients were 6.8 mm (95% CI 2.0-11.7 mm) and 7.2 mm (95% CI 3.111.3 mm) respectively in an adjusted regression model. SV motion did not differ between the ADT and nonADT patients in terms of interfractional motion of the SV tips and the SVs' center relative to the prostate gland. The ADT patients had significantly shorter SVs compared to the non-ADT patients, but no difference in SV motion was observed. SV interfractional motion should thus be compensated using the same planning margins, regardless of whether ADT is used.

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  • Efficacy and safety of 3 day versus 7 day cefditoren pivoxil regimens for acute uncomplicated cystitis: multicentre, randomized, open-label trial 査読

    Takuya Sadahira, Koichiro Wada, Motoo Araki, Ayano Ishii, Atsushi Takamoto, Yasuyuki Kobayashi, Masami Watanabe, Toyohiko Watanabe, Yasutomo Nasu, Hiromi Kumon

    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY   72 ( 2 )   529 - 534   2017年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Background: Fluoroquinolone-non-susceptible Escherichia coli isolated from patients with acute uncomplicated cystitis are a matter of increasing concern. Cefditoren pivoxil is an oral, beta-lactamase-stable, extended-spectrum cephalosporin that is effective against fluoroquinolone-non-susceptible bacteria.
    Objectives: To evaluate the clinical and microbiological efficacies of cefditoren pivoxil against acute uncomplicated cystitis and to determine the optimal duration of cefditoren pivoxil treatment.
    Methods: We compared 3 and 7 day regimens of cefditoren pivoxil in a multicentre, randomized, open-label study.
    Results: A total of 104 female patients with acute uncomplicated cystitis were enrolled and randomized into 3 day (n = 51) or 7 day (n = 53) treatment groups. At first visit, 94 bacterial strains were isolated from the 104 participants of which 81.7% (85/104) were E. coli. Clinical and microbiological efficacies were evaluated 59 days following administration of the final dose of cefditoren pivoxil. The clinical efficacies of the 3 and 7 day groups were 90.9% (40/44) and 93.2% (41/44), respectively (P = 1.000). The microbiological efficacies of the 3 and 7 day groups were 82.5% (33/40) and 90.2% (37/41), respectively (P = 0.349). There were no adverse events due to cefditoren pivoxil treatment, with the exception of a mild allergic reaction in one patient, after which the cefditoren pivoxil was exchanged for another antimicrobial.
    Conclusions: Cefditoren pivoxil is safe and effective for uncomplicated cystitis, with no significant differences in clinical and microbiological efficacies between 3 and 7 day regimens.

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  • The Prostate in Female? Clear Cell Adenocarcinoma in a Female Urethral Diverticulum 査読

    Takuya Sadahira, Yuuki Maruyama, Motoo Araki, Yasuyuki Kobayashi, Toyohiko Watanabe, Yasutomo Nasu

    UROLOGY   99   E25 - E26   2017年1月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    Urethral diverticular clear cell adenocarcinoma in a female patient is extremely rare. This tumor sometimes displays a characteristic image on magnetic resonance imaging, surrounding the urethra like the prostate does in men. We report a patient with this tumor who underwent cystourethrectomy and ileal conduit diversion. We think that imaging studies could be useful to make the appropriate diagnosis. UROLOGY 99: e25-e26, 2017. (C) 2016 Elsevier Inc.

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  • Distant Bystander Effect of REIC/DKK3 Gene Therapy Through Immune System Stimulation in Thoracic Malignancies 査読

    Ken Suzawa, Kazuhiko Shien, Huang Peng, Masakiyo Sakaguchi, Masami Watanabe, Shinsuke Hashida, Yuho Maki, Hiromasa Yamamoto, Shuta Tomida, Junichi Soh, Hiroaki Asano, Kazunori Tsukuda, Yasutomo Nasu, Hiromi Kumon, Shinichiro Miyoshi, Shinichi Toyooka

    ANTICANCER RESEARCH   37 ( 1 )   301 - 307   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT INST ANTICANCER RESEARCH  

    Background: Reduced expression in immortalized cell (REIC)/Dickkoph-3 (DKK3) is a tumor-suppressor gene, and its overexpression by adenovirus vector (Ad-REIC) exhibits a remarkable therapeutic effect on various human cancer types through a mechanism triggered by endoplasmic reticulum stress. Materials and Methods: We examined the direct anti-tumor effect of Ad-REIC gene therapy on lung cancer and malignant mesothelioma cell lines in vitro, and the distant bystander effect using immunocompetent mouse allograft models with bilateral flank tumors. Results: Ad-REIC treatment showed antitumor effect in many lung cancer and malignant mesothelioma cell lines in vitro. In an in vivo model, Ad-REIC treatment inhibited the growth not only of directly treated tumors but also of distant untreated tumors. By immunohistochemical analysis, infiltration of T-cells and natural killer (NK) cells and expression of the major histocompatibility complex (MHC) class I molecules were observed in bilateral tumors. Conclusion: Ad-REIC treatment not only had a direct antitumor effect but also an indirect bystander effect through stimulation of the immune system.

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  • Diagnostic Ureteroscopy for Cases Clinically Suspected of Carcinoma in Situ of the Upper Urinary Tract 査読

    Katsumi Sasaki, Koichiro Wada, Motoo Araki, Yasuyuki Kobayashi, Morito Sugimoto, Shin Ebara, Toyohiko Watanabe, Yasutomo Nasu

    ACTA MEDICA OKAYAMA   71 ( 3 )   227 - 232   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We elucidate the fate of cases clinically suspected of carcinoma in situ (Cis) of the upper tract with serial ureteroscopy. Of 143 patients who underwent ureteroscopy for suspected upper tract urothelial carcinoma (UTUC) between January 2008 and February 2016, 12 cases with consistently positive urine cytology and poorly detectable upper-tract malignancies by imaging were reviewed. In these 12 patients, 19 ureteroscopy procedures (25 renal units) were performed. Vesical random biopsy was performed before the 1st ureteroscopy to exclude malignancy of the bladder in all 12 cases. Median follow-up was 42 (13-67) months. Positive biopsy results at the 1st ureteroscopy were obtained in 3 (25%) patients and all were diagnosed wth Cis of the upper tract. Two (17%) of 9 patients who were negative or inconclusive at the 1st ureteroscopy were finally diagnosed as UTUC, but plural ureteroscopy procedures were needed for the diagnoses in both. Carcinoma of the bladder appeared in 5 (42%) patients during follow-up, despite the earlier ruling out of vesical malignancy. Four (33%) of those 5 patients never developed upper-tract urothelial carcinoma during follow-up. Caution is required before undertaking radical surgery for cases clinically suspected of Cis of the upper tract. In our experience, only 42% of such patients developed UTUC; another 33% eventually developed carcinoma of the bladder without UTUC.

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  • Value of percutaneous needle biopsy of small renal tumors in patients referred for cryoablation 査読

    Toshihiro Iguchi, Takao Hiraki, Hideo Gobara, Hiroyasu Fujiwara, Jun Sakurai, Yusuke Matsui, Motoo Araki, Yasutomo Nasu, Susumu Kanazawa

    MINIMALLY INVASIVE THERAPY & ALLIED TECHNOLOGIES   26 ( 2 )   86 - 91   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Purpose: To retrospectively evaluate the safety and diagnostic yield of needle biopsy of small renal tumors, and the clinical consequences of performing needle biopsy in patients referred for percutaneous cryoablation before their treatment.
    Material and methods: Biopsy was performed for 120 tumors (mean diameter, 2.2 cm) in 119 patients. All procedures were divided into diagnostic and non-diagnostic biopsies. Various variables were compared between the two groups. All cryoablation procedures were divided into two groups: procedures with or without simultaneous biopsy. The rates of benign or non-diagnostic tumors in each group were compared.
    Results: After performing 120 initial and eight repeat biopsies, Grade 1 bleedings occurred in 44 cases. Six tumors were non-diagnostic and 114 were pathologically diagnosed. There were no significant variables between the diagnostic and non-diagnostic biopsies. Unnecessary cryoablation was avoided in nine benign lesions by performing biopsy in advance. Cryoablation performed simultaneously with biopsy included significantly more benign or non-diagnostic tumors than cryoablation performed after biopsy (15.2% vs. 1.4%; p =.01).
    Conclusions: Percutaneous biopsy of small renal tumors referred for cryoablation was a safe procedure with high diagnostic yield. The confirmation of pathological diagnosis prior to cryoablation is necessary because patients with benign tumors can avoid unnecessary treatment.

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  • Development of a Novel “Optical Pu+C2:F23ncture Needle” Using Ultrathin High-Definition Image Guide System for Percutaneous Nephrolithotomy

    Tanimoto R, Wada K, Sasaki K, Sadahira T, Takamoto A, Araki M, Kobayashi K, Watanabe T, Nasu Y, Kumon H

    Videourology   31 ( 5 )   2017年

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  • Adenovirus vector carrying REICIDKK-3 gene: neoadjuvant intraprostatic injection for high-risk localized prostate cancer undergoing radical prostatectomy 査読

    H. Kumon, Y. Ariyoshi, K. Sasaki, T. Sadahira, M. Araki, S. Ebara, H. Yanai, M. Watanabe, Y. Nasu

    CANCER GENE THERAPY   23 ( 11 )   400 - 409   2016年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    As the First-In-Human study of in situ gene therapy using an adenovirus vector carrying the human REIC (reduced expression in immortalized cell)/Dkk-3 gene (Ad-REIC), we conducted neoadjuvant intraprostatic injections in patients with high-risk localized prostate cancer undergoing radical prostatectomy (RP). Patients with recurrence probability of 35% or more within 5 years following RP, as calculated by Kaftan's nomogram, were enrolled. Patients received two ultrasound-guided intratumoral injections at 2-week intervals, followed by RP 6 weeks after the second injection. After confirming the safety of the therapeutic interventions with initially planned three escalating doses of 1.0 x 10(10), 1.0 x 10(11) and 1.0 x 10(12) viral particles (vp) in 1.0-1.2 ml (n=3, 3 and 6), an additional higher dose of 3.0 x 10(12) vp in 3.6 ml (n=6) Was further studied. All four DLs including the additional dose level-4 (DL-4) were feasible with no adverse events, except for grade 1 or 2 transient fever. Laboratory toxicities were grade 1 or 2 elevated aspartate transaminase/alanine transaminase (n=4). Regarding antitumor activities, cytopathic effects (tumor degeneration With cytolysis and pyknosis) and remarkable tumor-infiltrating lymphocytes in the targeted tumor areas were detected in a clear dose-dependent manner. Consequently, biochemical recurrence-free survival in DL-4 was significantly more favorable than in patient groups DL-1+2+2:

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  • Dynamin2 GTPase contributes to invadopodia formation in invasive bladder cancer cells 査読

    Yubai Zhang, Maya Nolan, Hiroshi Yamada, Masami Watanabe, Yasutomo Nasu, Kohji Takei, Tetsuya Takeda

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   480 ( 3 )   409 - 414   2016年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Cancer cell invasion is mediated by actin-based membrane protrusions termed invadopodia. Invadopodia consist of "core" F-actin bundles associated with adhesive and proteolytic machineries promoting cell invasion by degrading extracellular matrix (ECM). Formation of the F-actin core in invadopodia is regulated by various actin-binding proteins including Arp2/3 complex and cortactin. Dynamin GTPase localizes to the invadopodia and is implicated in cancer cell invasion, but its precise role at the invadopodia remained elusive.
    In this study, we examined the roles of dynamin at the invadopodia of bladder cancer cells. Although all three dynamin isoforms (dynamin1, 2 and 3) are expressed in human bladder cancer cell line T24, only dynamin2 localizes to the invadopodia. Inhibition of dynamin2 function, using either RNA interference (RNAi) or the dynamin specific inhibitor Dynasore, caused defects in invadopodia formation and suppressed invasive activity of 124 bladder cancer cells. Structure-function analysis using dynamin2 deletion fragments identified the proline/arginine-rich domain (PRD) of dynamin2 as indispensable for invadopodia formation and invasiveness of 124 cells. Thus, dynamin2 contributes to bladder cancer invasion by controlling invadopodia formation in bladder cancer cells and may prove a valuable therapeutic target. (C) 2016 Elsevier Inc. All rights reserved.

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  • A Phase II Clinical Trial Evaluating the Preventive Effectiveness of Lactobacillus Vaginal Suppositories in Patients with Recurrent Cystitis 査読

    Koichiro Wada, Shinya Uehara, Ayano Ishii, Takuya Sadahira, Masumi Yamamoto, Ritsuko Mitsuhata, Atsushi Takamoto, Motoo Araki, Yasuyuki Kobayashi, Masami Watanabe, Toyohiko Watanabe, Katsuyuki Hotta, Yasutomo Nasu

    ACTA MEDICA OKAYAMA   70 ( 4 )   299 - 302   2016年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    Urinary tract infections (UTIs) are the most common bacterial infections in women, and many patients experience frequent recurrence. The aim of this report is to introduce an on-going prospective phase II clinical trial performed to evaluate the preventive effectiveness of Lactobacillus vaginal suppositories for prevention of recurrent cystitis. Patients enrolled in this study are administered vaginal suppositories containing the GAI 98322 strain of Lactobacillus crispatus every 2 days or 3 times a week for one year. The primary endpoint is recurrence of cystitis and the secondary endpoints are adverse events. Recruitment began in December 2013 and target sample size is 20 participants.

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  • The Efficacy of Rituximab in High-risk Renal Transplant Recipients 査読

    Motoo Araki, Koichiro Wada, Yosuke Mitsui, Risa Kubota, Takashi Yoshioka, Yuichi Ariyoshi, Yasuyuki Kobayashi, Masashi Kitagawa, Katsuyuki Tanabe, Hiroshi Sugiyama, Jun Wada, Masami Watanabe, Toyohiko Watanabe, Katsuyuki Hotta, Yasutomo Nasu

    ACTA MEDICA OKAYAMA   70 ( 4 )   295 - 297   2016年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    Although graft survival following renal transplantation (RTx) has improved, outcomes following highrisk RTx are variable. Preexisting antibodies, including donor-specific antibodies (DSA), play an important role in graft dysfunction and survival. We have designed a study to investigate the safety and efficacy of anti-CD20 monoclonal antibodies (rituximab) in high-risk RTx recipients. Major eligibility criteria include: 1) major and minor ABO blood group mismatch, 2) positive DSA. Thirty-five patients will receive 200 mg/body of rituximab. The primary endpoint is the incidence of B cell depletion. This study will clarify whether rituximab is efficacious in improving graft survival in high-risk RTx recipients.

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  • Novel REIC/Dkk-3-encoding adenoviral vector as a promising therapeutic agent for pancreatic cancer 査読

    H. Sawahara, H. Shiraha, D. Uchida, H. Kato, T. Nagahara, M. Iwamuro, J. Kataoka, S. Horiguchi, M. Watanabe, M. Sakaguchi, A. Takaki, K. Nouso, Y. Nasu, H. Kumon, H. Okada

    CANCER GENE THERAPY   23 ( 8 )   278 - 283   2016年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Reduced expression in immortalized cells (REIC)/dickkopf-3 (Dkk-3), a tumor suppressor gene, is downregulated in various cancers. We previously reported the tumor-inhibitory effects of the REIC/Dkk-3 gene, delivered by a conventional adenoviral vector (Ad-CAG-REIC) in pancreatic cancer. Here, we developed an Ad-REIC vector with a novel gene expression system, termed the super gene expression (SGE) system, and assessed its therapeutic effects relative to those of Ad-CAG-REIC in pantreatic cancer cells. Human pancreatic cancer cell lines ASPC1 and MIAPaCa2 were used. REIC/Dkk-3 expression was assessed by western blot analysis. Relative cell viability and apoptotic effects were examined in vitro. The anti-tumor effects of Ad-REIC treatment were assessed in the mouse xenograft model. Compared with Ad-CAG-REIC, Ad-SGE-REIC elicited a significant increase in REIC protein expression in the cells studied, Relative to Ad-CAG-REIC, Ad-SGE-REIC reduced cell viability and induced apoptosis in the ASPC1 and MIAPaCa2 cell lines in vitro, and achieved superior tumor growth inhibition in the mouse xenograft model. Compared with conventional Ad-REIC agents, Ad-SGE-REIC provided enhanced inhibitory effects against tumor growth. Our results indicate that Ad-SGE-REIC is an innovative therapeutic tool for pancreatic cancer.

    DOI: 10.1038/cgt.2016.31

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  • Multi-institutional retrospective analysis of learning curves on dosimetry and operation time befor and after introduction of intraoperatively built custom-linked seeds in prostate brachytherapy

    Ishiyama H, Satoh T, Yorozu A, Saito S, Kataoka M, Hashine K, Nakamura R, Tanji S, Masui K, Okihara K, Ohashi T, Momma T, Aoki M, Miki K, Kato M, Morita M, Katayama N, Nasu Y, Kawanaka T, Fukumori T, Ito F, Shiroki R, Baba Y, Inadome A, Yoshioka Y, Takayama H, Hayakawa K

    J Radiat Res   57 ( 1 )   68 - 74   2016年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Clinical analysis of bacterial strain profiles isolated from urinary tract infections:A 30-year study

    Wada K, Uehara S, Yamamoto M, Sadahira T, Mitsuhata R, Araki M, Kobayashi Y, Ishii A, Kariyama R, Watanabe T, Nasu Y, Kumon H

    J Infect Chemother   22 ( 7 )   478 - 482   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Synergistic anti-pancreatic cancer immunological effects by treatment with reduced expression in immortalized cells/dickkopf-3 protein and peripheral blood mononuclear cells 査読

    Daisuke Uchida, Hidenori Shiraha, Hironari Kato, Hiroaki Sawahara, Teruya Nagahara, Masaya Iwamuro, Junro Kataoka, Shigeru Horiguchi, Masami Watanabe, Akinobu Takaki, Kazuhiro Nouso, Yasutomo Nasu, Hiromi Kumon, Kazuhide Yamamoto

    JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY   31 ( 6 )   1154 - 1159   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Background and AimReduced expression in immortalized cells/dickkopf-3 (REIC/DKK3) is a reported tumor suppressor gene and has potential to become an innovative therapy for various cancers. We examined the antitumor immunological effects of human REIC/DKK3 protein against pancreatic cancer.
    MethodsActivation of extracellular signal-regulated kinases 1 and 2, mammalian target of rapamycin, and signal transducer and activator of transcription 3 by REIC/DKK3 protein was assessed in human peripheral blood mononuclear cells using immunoblotting. Pancreatic cancer cell lines (AsPC-1 and MIA Paca-2) were cocultured with peripheral blood mononuclear cells, and the anticancer effects of REIC/DKK3 protein were assessed using the methyl thiazole tetrazolium, cytotoxicity, and enzyme-linked immunospot assays. The antitumor immunological effects of the combined treatment with REIC/DKK3 protein and peripheral blood mononuclear cells were also assessed in a pancreatic cancer model using non-obese diabetic/severe combined immunodeficiency mice.
    ResultsThe REIC/DKK3 protein activated extracellular signal-regulated kinases 1 and 2, mammalian target of rapamycin, and signal transducer and activator of transcription 3 in peripheral blood mononuclear cells. REIC/DKK3 protein inhibited in vitro cancer cell viability and enhanced cytotoxicity when incubated with peripheral blood mononuclear cells. REIC/DKK3 protein induced significant production of interferon gamma from lymphocytes incubated with pancreatic cancer cells, indicating that CD8+ T cells were activated in the peripheral blood mononuclear cells when cocultured with AsPC-1 and MIA Paca-2 in the presence of REIC/DKK3 protein. Combined treatment with REIC/DKK3 protein and peripheral blood mononuclear cells produced in vivo anticancer immunostimulatory effects on pancreatic cancer cells.
    ConclusionsThe REIC/DKK3 protein and peripheral blood mononuclear cells synergistically enhanced anticancer immunological effects against pancreatic cancer cells. The observed immunomodulatory effect of combined treatment likely occurs in adenovirus-mediated REIC/DKK3 gene therapy and provides important clues to the therapeutic mechanisms involving immune cells.

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  • A Case of Metastatic Urachal Cancer Including a Neuroendocrine Component Treated with Gemcitabine, Cisplatin and Paclitaxel Combination Chemotherapy 査読

    Shin Ebara, Yasuyuki Kobayashi, Katsumi Sasaki, Motoo Araki, Morito Sugimoto, Koichirou Wada, Kei Fujio, Atsushi Takamoto, Toyohiko Watanabe, Hiroyuki Yanai, Yasutomo Nasu

    ACTA MEDICA OKAYAMA   70 ( 3 )   223 - 227   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    The present case report describes a case of recurrent and advanced urachal carcinoma including neuroendocrine features with iliac bone metastasis after partial cystectomy and adjuvant chemotherapy consisting of irinotecan and cisplatin in a 32-year-old man. He received gemcitabine/cisplatin/paclitaxel (GCP) combination chemotherapy, consisting of gemcitabin (1,000mg/m(2)) on day 1, 8, cisplatin (70mg/m(2)) on day 1, and paclitaxel (80mg/m(2)) on day 1 and 8. After three cycles of chemotherapy, PET-CT showed complete regression of the disease. So the patient underwent total cystoure-threctomy, and histological examination showed an almost complete pathological response. External beam radiation therapy was also given to the ileac bone metastasis regions. However, PET-CT taken 17 months after the external beam radiation showed multiple lung metastases. He received GCP chemotherapy again, which resulted in a complete response again after three cycles of chemotherapy. This is the first report on GCP chemotherapy used not only as a salvage chemotherapy but also as a rechallenge regimen for metastatic urachal cancer including a neuroendocrine component.

    DOI: 10.18926/AMO/54423

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  • Effectiveness and Safety of Ureteroscopic Holmium Laser Lithotripsy for Upper Urinary Tract Calculi in Elderly Patients 査読

    Takashi Yoshioka, Hideo Otsuki, Shinya Uehara, Toshihiro Shimizu, Wataru Murao, Koji Fujio, Kei Fujio, Koichiro Wada, Motoo Araki, Yasutomo Nasu

    ACTA MEDICA OKAYAMA   70 ( 3 )   159 - 166   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    Upper urinary tract calculi are common; however, there is no recommended treatment selection for elderly patients. Ureteroscopic holmium laser lithotripsy (URS lithotripsy) is minimally invasive, and it provides a high stone-free rate (SFR) treatment for upper urinary tract calculi. Here, we retrospectively evaluated the surgical outcomes of URS lithotripsy after dividing the 189 cases into 3 groups by patient age: the '< 65 group' (< 65 years old, n=108), the '65-74 group' (65-74 years old, n=42), and the '>= 75 group' (>= 75 years old, n=39). The patients 'characteristics, stone status, and perioperative outcomes were assessed. The 65-74 group and the >= 75 group had a significantly higher prevalence of hypertension compared to the < 65 group. Compared to the < 65 group, the 65-74 group had a significantly higher prevalence of hyperlipidemia, and the >= 75 group had significantly higher the American Society of Anesthesiologists (ASA) scores. Despite these preoperative risk factors, SFR and postoperative pyelonephritis in the 65-74 group and the >= 75 group were similar to those of the < 65 group. In conclusion, URS lithotripsy is the preferred treatment for upper urinary tract calculi, even for elderly patients who have multiple preoperative risk factors.

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  • The induction of antigen-specific CTL by in situ Ad-REIC gene therapy 査読

    Y. Ariyoshi, M. Watanabe, S. Eikawa, C. Yamazaki, T. Sadahira, T. Hirata, M. Araki, S. Ebara, Y. Nasu, H. Udono, H. Kumon

    GENE THERAPY   23 ( 5 )   408 - 414   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    An adenovirus vector carrying the human Reduced Expression in Immortalized Cell (REIC)/Dkk-3 gene (Ad-REIC) mediates simultaneous induction of cancer-selective apoptosis and augmentation of anticancer immunity. In our preclinical and clinical studies, in situ Ad-REIC gene therapy showed remarkable direct and indirect antitumor effects to realize therapeutic cancer vaccines. We herein aimed to confirm the induction of tumor-associated antigen-specific cytotoxic T lymphocytes (CTLs) by Ad-REIC. Using an ovalbumin (OVA), a tumor-associated antigen, expressing E.G7 tumor-bearing mouse model, we investigated the induction and expansion of OVA-specific CTLs responsible for indirect, systemic effects of Ad-REIC. The intratumoral administration of Ad-REIC mediated clear antitumor effects with the accumulation of OVA-specific CTLs in the tumor tissues and spleen. The CD86-positive dendritic cells (DCs) were upregulated in the tumor draining lymph nodes of Ad-REIC-treated mice. In a dual tumor-bearing mouse model in the left and right back, Ad-REIC injection in one side significantly suppressed the tumor growth on both sides and significant infiltration of OVA-specific CTLs into non-injected tumor was also detected. Consequently, in situ Ad-REIC gene therapy is expected to realize a new-generation cancer vaccine via anticancer immune activation with DC and tumor antigen-specific CTL expansion.

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  • Factors Predicting Adhesion between Renal Capsule and Perinephric Adipose Tissue in Partial Nephrectomy 査読

    Yasuyuki Kobayashi, Hiroaki Kurahashi, Yuko Matsumoto, Koichiro Wada, Katsumi Sasaki, Motoo Araki, Shin Ebara, Toyohiko Watanabe, Yasutomo Nasu

    ACTA MEDICA OKAYAMA   70 ( 2 )   69 - 74   2016年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    In minimally invasive partial nephrectomy (MIPN), it is important to preoperatively predict the degree of difficulty of tumor resection. When severe adhesions occur between the renal capsule and perinephric adipose tissue, detachment can be difficult. Preoperative prediction of adhesion is thought to be useful in the selection of surgical procedure. Subjects were 63 patients of a single surgeon who had received MIPN between April 2008 and August 2013 at Okayama University Hospital. Of these patients, this study followed 47 in whom the presence or absence of adhesions between the renal capsule and perinephric adipose tissue was confirmed using intraoperative videos. Data collected included: sex, BMI, CT finding (presence of fibroids in perinephric adipose tissue), comorbidities and lifestyle. Adhesion was observed in 7 patients (14.9). The mean operative time was 291.6min in the adhesion group, and 226.3min in the group without. The increased time in the adhesions group was significant (p<0.05). Predictive factors were a positive CT finding for fibroid structure and comorbidity of hypertension (p<0.05). In MIPN, difficulty of surgery can be affected by the presence of adhesion of the perinephric adipose tissue. Predicting such adhesion from preoperative CT is thus important.

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  • Development of a Novel REIC/DKK-3-Encoding Adenoviral Agent: Its Robust and Promising Therapeutic Effects in Pancreatic Cancer 査読

    Daisuke Uchida, Hidenori Shiraha, Hironari Kato, Sawahara Hiroaki, Masakiyo Sakaguchi, Masami Watanabe, Yasutomo Nasu, Hiromi Kumon, Hiroyuki Okada

    GASTROENTEROLOGY   150 ( 4 )   S294 - S294   2016年4月

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    記述言語:英語   出版者・発行元:W B SAUNDERS CO-ELSEVIER INC  

    DOI: 10.1016/S0016-5085(16)31025-3

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  • Promising Therapeutic Efficacy of a Novel REIC/Dkk-3 Expressing Adenoviral Vector for Hepatocellular Carcinoma 査読

    Sawahara Hiroaki, Hidenori Shiraha, Daisuke Uchida, Masakiyo Sakaguchi, Masami Watanabe, Yasutomo Nasu, Hiromi Kumon, Hiroyuki Okada

    GASTROENTEROLOGY   150 ( 4 )   S1153 - S1153   2016年4月

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    記述言語:英語   出版者・発行元:W B SAUNDERS CO-ELSEVIER INC  

    DOI: 10.1016/S0016-5085(16)33891-4

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  • Comparison of implant quality between intraoperatively built custom-linked seeds and loose seeds in permanent prostate brachytherapy using sector analysis

    Katayama N, Takemoto M, Takamoto A, Ihara H, Katsui K, Ebara S, Nasu Y, Kanazawa S

    J Radiat Res   57 ( 4 )   393 - 399   2016年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • The gender specific risk factors for prolonged hospitalization due to acute pyelonephritis in a Japanese tertiary emergency center 査読

    Risa Muneishi, Ryuta Tanimoto, Koichiro Wada, Philip Hsiao, Jun Eguchi, Motoo Araki, Toyohiko Watanabe, Yasutomo Nasu, Naoki Akebi

    JOURNAL OF INFECTION AND CHEMOTHERAPY   22 ( 1-2 )   108 - 111   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Objectives: The aim of this study is to characterize the potential differences between male and female patients with acute pyelonephritis (AP) and to predict the severity of AP based on the length of hospital stay.
    Methods: We conducted a retrospective medical chart review of 172 consecutive adult patients who were hospitalized in Tsuyama Central Hospital due to AP from January 2007 through June 2012. We analyzed the length of hospital stay by the proportional hazard model.
    Results: A total of 172 patients were identified who were admitted to our hospital with a diagnosis of AP. Of them, 62% (106/172) were female. Except for urological malignancy, there was no significant difference between men and women in underlying disease. Out of 26 variables, univariate analysis in male showed that only urolithiasis (OR 1.75, p = 0.0294) was significantly associated with longer hospital stay, while septic shock (OR 3.18, P = 0.003), urological malignancy (OR 2.94, P = 0.002), age over 65 (OR 1.66, p = 0.018) and neurogenic bladder (OR 1.92, p = 0.014) were all associated with longer hospital stay in female patients.
    Conclusions: This is the first report to identify the risk factors for prolonged hospital stay for the patients who were admitted with AP in the Japanese population. The risk factors causing prolonged hospital stay were totally different between males and females. Reviewing the medical history based on sex gender might enable a clinician to predict the severity of acute pyelonephritis during the initial evaluation. (C) 2015, Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jiac.2015.11.007

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  • An Open-Label, Randomized Phase II Trial of Personalized Peptide Vaccination in Patients with Bladder Cancer that Progressed after Platinum-Based Chemotherapy 査読

    Masanori Noguchi, Kazumasa Matsumoto, Hirotsugu Uemura, Gaku Arai, Masatoshi Eto, Seiji Naito, Chikara Ohyama, Yasutomo Nasu, Masatoshi Tanaka, Fukuko Moriya, Shigetaka Suekane, Satoko Matsueda, Nobukazu Komatsu, Tetsuro Sasada, Akira Yamada, Tatsuyuki Kakuma, Kyogo Itoh

    CLINICAL CANCER RESEARCH   22 ( 1 )   54 - 60   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC CANCER RESEARCH  

    Purpose: The prognosis of platinum-based chemotherapy-resistant metastatic urothelial cancer of the bladder remains poor. Personalized selection of the right peptides for each patient could be a novel approach for a cancer vaccine to boost anticancer immunity.
    Experimental Design: In this randomized, open-label, phase II study, patients ages >= 18 years with progressive bladder cancer after first-line platinum-based chemotherapy were randomly assigned (1: 1) to receive personalized peptide vaccination (PPV) plus best supportive care (BSC) or BSC. PPV treatment used a maximum of four peptides chosen from 31 candidate peptides according to human leukocyte antigen types and peptide-reactive IgG titers, for 12 s.c. injections (8 injections, weekly; 4 injections, bi-weekly). The primary endpoint was progression-free survival (PFS). Secondary end-points were overall survival (OS), immune response, and toxicity.
    Results: Eighty patients were randomly assigned to receive either PPV plus BSC (n = 39) or BSC (n = 41). No significant improvement in PFS was noted [HR, 0.7; 95% confidence interval (CI), 0.4-1.2, P = 0.17]. For the secondary endpoints, PPV plus BSC significantly prolonged OS compared with BSC (HR, 0.58; 95% CI, 0.34-0.99, P = 0.049), with median OS of 7.9 months (95% CI, 3.5-12.0) in the PPV plus BSC and 4.1 months (95% CI, 2.8-6.9) in the BSC. PPV treatment was well tolerated, without serious adverse drug reactions.
    Conclusions: PPV could not prolong PFS, but OS appeared to be improved with low toxicity and immune responses. Further large-scale, randomized trials are needed to confirm these results. (C) 2015 AACR.

    DOI: 10.1158/1078-0432.CCR-15-1265

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  • Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity 査読

    Kazuhiko Ochiai, Masami Morimatsu, Yuiko Kato, Toshina Ishiguro-Oonuma, Chihiro Udagawa, Oumaporn Rungsuriyawiboon, Daigo Azakami, Masaki Michishita, Yuichi Ariyoshi, Hideo Ueki, Yasutomo Nasu, Hiromi Kumon, Masami Watanabe, Toshinori Omi

    ONCOTARGET   7 ( 3 )   3273 - 3286   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IMPACT JOURNALS LLC  

    REIC/DKK-3 is a tumor suppressor, however, its intracellular physiological functions and interacting molecules have not been fully clarified. Using yeast two-hybrid screening, we found that small glutamine-rich tetratricopeptide repeat-containing protein a (SGTA), known as a negative modulator of cytoplasmic androgen receptor (AR) signaling, is a novel interacting partner of REIC/DKK-3. Mammalian two-hybrid and pull-down assay results indicated that the SGTA-REIC/DKK-3 interaction involved the N-terminal regions of both REIC/DKK-3 and SGTA and that REIC/DKK-3 interfered with the dimerization of SGTA, which is a component of the AR complex and a suppressor of dynein motor-dependent AR transport and signaling. A reporter assay in human prostate cancer cells that displayed suppressed AR signaling by SGTA showed recovery of AR signaling by REIC/DKK-3 expression. Considering these results and our previous data that REIC/DKK-3 interacts with the dynein light chain TCTEX-1, we propose that the REIC/DKK-3 protein interferes with SGTA dimerization, promotes dynein-dependent AR transport and then upregulates AR signaling.

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  • Feasibility of Neoadjuvant Ad-REIC Gene Therapy in Patients with High-Risk Localized Prostate Cancer Undergoing Radical Prostatectomy 査読

    Hiromi Kumon, Katsumi Sasaki, Yuichi Ariyoshi, Takuya Sadahira, Motoo Araki, Shin Ebara, Hiroyuki Yanai, Masami Watanabe, Yasutomo Nasu

    CTS-CLINICAL AND TRANSLATIONAL SCIENCE   8 ( 6 )   837 - 840   2015年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    In a phase I/IIa study of in situ gene therapy using an adenovirus vector carrying the human REIC/Dkk-3 gene (Ad-REIC), we assessed the inhibitory effects of cancer recurrence after radical prostatectomy (RP), in patients with high risk localized prostate cancer (PCa). After completing the therapeutic interventions with initially planned three escalating doses of 1.0 x 10(10), 1.0 x 10(11), and 1.0 x 10(12) viral particles (VP) in 1.0-1.2 mL (n = 3, 3, and 6), an additional higher dose of 3.0 x 10(12) VP in 3.6 mL (n = 6) was further studied. Patients with recurrence probability of 35% or more within 5 years after RP as calculated by Kattan's nomogram, were enrolled. They received two ultrasound-guided intratumoral injections at 2-week intervals, followed by RP 6 weeks after the second injection. Based on the findings of MRI and biopsy mapping, as a rule, one track injection to the most prominent cancer area was given to initial 12 patients and 3 track injections to multiple cancer areas in additional 6 patients. As compared to the former group, biochemical recurrence-free survival of the latter showed a significantly favorable outcome. Neoadjuvant Ad-REIC, mediating simultaneous induction of cancer selective apoptosis and augmentation of antitumor immunity, is a feasible approach in preventing cancer recurrence after RP. (199)

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  • Distant Bystander Effect of REIC/Dkk-3 Gene Therapy through Immune System Stimulation in a Murine Model of Thoracic Malignancies 査読

    Suzawa Ken, Shien Kazuhiko, Huang Peng, Sakaguchi Masakiyo, Watanabe Masami, Hashida Shinsuke, Soh Junichi, Yamamoto Hiromasa, Maki Yuho, Asano Hiroaki, Tsukuda Kazunori, Nasu Yasutomo, Kumon Hiromi, Miyoshi Shinichiro, Toyooka Shinichi

    JOURNAL OF THORACIC ONCOLOGY   10 ( 9 )   S599   2015年9月

  • A vaccine strategy with multiple prostatic acid phosphatase (PAP)-fused cytokines for prostate cancer treatment. 査読

    Kei Fujio, Masami Watanabe, Hideo Ueki, Shun-Ai Li, Rie Kinoshita, Kazuhiko Ochiai, Junichiro Futami, Toyohiko Watanabe, Yasutomo Nasu, Hiromi Kumon

    Oncology Reports   33 ( 4 )   1585 - 1592   2015年4月

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    記述言語:英語  

    DOI: 10.3892/or.2015.3770

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  • Ad-REIC Gene Therapy: Promising Results in a Patient with Metastatic CRPC Following Chemotherapy. 査読

    Hiromi Kumon, Katsumi Sasaki, Yuichi Ariyoshi, Takuya Sadahira, Shin Ebara, Takao Hiraki, Susumu Kanazawa, Hiroyuki Yanai, Masami Watanabe, Yasutomo Nasu

    Clinical Medicine Insights: Oncology   9   31 - 38   2015年3月

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    記述言語:英語  

    DOI: 10.4137/CMO.S23252

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  • Integrin antagonist augments the therapeutic effect of adenovirus-mediated REIC/Dkk-3 gene therapy for malignant glioma. 査読

    Yosuke Shimazu, Kazuhiko Kurozumi, Tomotsugu Ichikawa, Kentaro Fujii, Manabu Onishi, Joji Ishida, Tetsuo Oka, Masami Watanabe, Yasutomo Nasu, Hiromi Kumon, Isao Date

    Gene Therapy   22 ( 2 )   146 - 154   2015年2月

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    記述言語:英語  

    DOI: 10.1038/gt.2014.100

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  • Genome-wide association study identified SNP on 15q24 associated with bladder cancer risk in Japanese population

    Matsuda K, Takahashi A, Middlebrooks C.D, Obara W, Nasu Y, Inoue K, Tamura K, Yamasaki I, Naya Y, Tanikawa C, Cui R, Figueroa J.D, Silverman D.T, Rothman N, Namiki M, Tomita Y, Nishiyama H, Kohri K, Deguchi T, Nakagawa M, Yokoyama M, Miki T, Kumon H, Fujioka T, Ludmila P.O, Kubo M, Nakamura Y, Shuin T

    Human Molecular Genetics   24 ( 4 )   1177 - 1184   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Significant association between the Axin2 rs2240308 single nucleotide polymorphism and the incidence of prostate cancer 査読

    Chao Ma, Chunxiao Liu, Peng Huang, Haruki Kaku, Jie Chen, Kai Guo, Hideo Ueki, Akiko Sakai, Yasutomo Nasu, Hiromi Kumon, Kenji Shimizu, Masami Watanabe

    ONCOLOGY LETTERS   8 ( 2 )   789 - 794   2014年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    The Wnt signaling pathway plays a crucial role in human cancer development, and axis inhibition protein 2 (Axin2) is a master scaffold protein involved in Wnt signaling. Axin2 negatively regulates Wnt signaling and acts as a tumor suppressor protein. The present study evaluated the association between the Axin2 single nucleotide polymorphism (SNP) rs2240308 [guanine (G)/adenine (A)] and the incidence of prostate cancer. In total, 103 patients with prostate cancer and 100 cancer-free control males were included in this case-control study, and were genotyped using the genomic DNA extracted from peripheral blood samples. The results revealed a higher incidence of prostate cancer in the subjects with the homozygous GG genotype and a reduced cancer incidence in the patients with the GA genotype of the rs2240308 SNP (G/A) in the Axin2 gene. T-he adjusted odds ratio for carriers with the GA genotype was 0.377 (95% CI, 0.206-0.688; P=0.001) and that for the AA genotype was 0.830 (95% CI, 0.309-2.232; P=0.712) compared with the GG genotype. Therefore, the GA genotype was found to exhibit a protective effect that decreased the risk of prostate cancer. To the best of our knowledge, this is the first study to demonstrate the significant association between this SNP (rs2240308, G/A) and the risk of prostate cancer. This association indicates the possibility that the variations in the Axin2 gene in this position may play a significant role in promoting the development of cancer in the prostate. We believe that the Axin2 SNP (rs2240308) could be a useful biomarker for the predisposition and early diagnosis of the disease.

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  • Immunological aspects of REIC/Dkk-3 gene therapy : the mechanism of the robust anti-tumor effects. 査読

    Masami Watanabe, Peng Huang, Fernando Abarzua, Haruki Kaku, Katsumi Sasaki, Hideo Ueki, Toyohiko Wananabe, Yasutomo Nasu, Hiromi Kumon

    JOURNAL OF GENE MEDICINE   16 ( 7-8 )   226 - 227   2014年7月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

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  • Dramatic Increase in Expression of a Transgene by Insertion of Promoters Downstream of the Cargo Gene 査読

    Masakiyo Sakaguchi, Masami Watanabe, Rie Kinoshita, Haruki Kaku, Hideo Ueki, Junichiro Futami, Hitoshi Murata, Yusuke Inoue, Shun-Ai Li, Peng Huang, Endy Widya Putranto, I. Made Winarsa Ruma, Yasutomo Nasu, Hiromi Kumon, Nam-ho Huh

    MOLECULAR BIOTECHNOLOGY   56 ( 7 )   621 - 630   2014年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:HUMANA PRESS INC  

    For expression of genes in mammalian cells, various vectors have been developed using promoters including CMV, EF-1 alpha, and CAG promoters and have been widely used. However, such expression vectors sometimes fail to attain sufficient expression levels depending on the nature of cargo genes and/or on host cell types. In the present study, we aimed to develop a potent promoter system that enables high expression levels of cargo genes ubiquitously in many different cell types. We found that insertion of an additional promoter downstream of a cargo gene greatly enhanced the expression levels. Among the constructs we tested, C-TSC cassette (C: CMV-RU5' located upstream; TSC: another promoter unit composed of triple tandem promoters, hTERT, SV40, and CMV, located downstream of the cDNA plus a polyadenylation signal) had the most potent capability, showing far higher efficiency than that of potent conventional vector systems. The results indicate that the new expression system is useful for production of recombinant proteins in mammalian cells and for application as a gene therapeutic measure.

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  • Potential of adenovirus-mediated REIC/Dkk-3 gene therapy for use in the treatment of pancreatic cancer 査読

    Daisuke Uchida, Hidenori Shiraha, Hironari Kato, Teruya Nagahara, Masaya Iwamuro, Junro Kataoka, Shigeru Horiguchi, Masami Watanabe, Akinobu Takaki, Kazuhiro Nouso, Yasutomo Nasu, Takahito Yagi, Hiromi Kumon, Kazuhide Yamamoto

    JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY   29 ( 5 )   973 - 983   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Background and AimThe reduced expression in immortalized cells REIC/the dickkopf 3 (Dkk-3) gene, tumor suppressor gene, is downregulated in various malignant tumors. In a prostate cancer study, an adenovirus vector carrying the REIC/Dkk-3 gene (Ad-REIC) induces apoptosis. In the current study, we examined the effects of REIC/Dkk-3 gene therapy in pancreatic cancer.
    MethodsREIC/Dkk-3 expression was assessed by immunoblotting and immunohistochemistry in the pancreatic cancer cell lines (ASPC1, MIAPaCa2, Panc1, BxPC3, SUIT-2, KLM1, and T3M4) and pancreatic cancer tissues. The Ad-REIC agent was used to investigate the apoptotic effect in vitro and antitumor effects in vivo. We also assessed the therapeutic effects of Ad-REIC therapy with gemcitabine.
    ResultsThe REIC/Dkk-3 expression was lost in the pancreatic cancer cell lines and decreased in pancreatic cancer tissues. Ad-REIC induced apoptosis and inhibited cell growth in the ASPC1 and MIAPaCa2 lines in vitro, and Ad-REIC inhibited tumor growth in the mouse xenograft model using ASPC1 cells. The antitumor effect was further enhanced in combination with gemcitabine. This synergistic effect may be caused by the suppression of autophagy via the enhancement of mammalian target of rapamycin signaling.
    ConclusionsAd-REIC induces apoptosis and inhibits tumor growth in pancreatic cancer cell lines. REIC/Dkk-3 gene therapy is an attractive therapeutic tool for pancreatic cancer.

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  • Adenovirus-mediated REIC/Dkk-3 gene therapy: Development of an autologous cancer vaccination therapy (Review) 査読

    Masami Watanabe, Yasutomo Nasu, Hiromi Kumon

    ONCOLOGY LETTERS   7 ( 3 )   595 - 601   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    Reduced expression in immortalized cells (REIC)/Dickkopf (Dkk)-3 is a tumor suppressor and therapeutic gene and has been studied with respect to the application of cancer gene therapy. Our previous studies demonstrated that the intratumoral injection of an adenovirus vector carrying the human REIC/Dkk-3 gene (Ad-REIC) suppresses tumor growth in mouse models of prostate, breast and testicular cancer and malignant mesothelioma. The mechanisms underlying these antitumor therapeutic effects have only been clarified recently. It has been demonstrated that Ad-REIC treatment inhibits cancer progression via the upregulation of systemic anticancer immunity. Under experimental conditions, autologous cancer vaccination via cancer-specific apoptosis and anticancer immune activation is a possible therapeutic mechanism. The robust anticancer effects observed in previous preclinical studies support the clinical utility of Ad-REIC. At present, a phase I-IIa study of Ad-REIC gene therapy in prostate cancer patients is ongoing. The current study reviews the observations of previous fundamental studies and summarizes the anticancer mechanisms of intratumoral Ad-REIC treatment in terms of cancer vaccination.

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  • A novel gene expression system strongly enhances the anticancer effects of a REIC/Dkk-3-encoding adenoviral vector 査読

    Masami Watanabe, Masakiyo Sakaguchi, Rie Kinoshita, Haruki Kaku, Yuichi Ariyoshi, Hideo Ueki, Ryuta Tanimoto, Shin Ebara, Kazuhiko Ochiai, Junichiro Futami, Shun-Ai Li, Peng Huang, Yasutomo Nasu, Nam-Ho Huh, Hiromi Kumon

    ONCOLOGY REPORTS   31 ( 3 )   1089 - 1095   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    Gene expression systems with various promoters, including the cytomegalovirus (CMV) promoter, have been developed to increase the gene expression in a variety of normal and cancer cells. In particular, in the clinical trials of cancer gene therapy, a more efficient and robust gene expression system is required to achieve sufficient therapeutic outcomes. By inserting the triple translational enhancer sequences of human telomerase reverse transcriptase (hTERT), Simian virus 40 (SV40) and CMV downstream of the sequence of the BGH polyA, we were able to develop a novel gene expression system that significantly enhances the expression of the genes of interest. We termed this novel gene expression cassette the super gene expression (SGE) system, and herein verify the utility of the SGE cassette for a replication-deficient adenoviral vector. We newly developed an adenoviral vector expressing the tumor suppressor, reduced expression in immortalized cells (REIC)/Dickkopf-3 (Dkk-3), based on the CMV promoter-driven SGE system (Ad-SGE-REIC) and compared the therapeutic utility of Ad-SGE-REIC with that of the conventional adenoviral vectors (Ad-CMV-REIC or Ad-CAG-REIC). The results demonstrated that the CMV promoter-SGE system allows for more potent gene expression, and that the Ad-SGE-REIC is superior to conventional adenoviral systems in terms of the REIC protein expression and therapeutic effects. Since the SGE cassette can be applied for the expression of various therapeutic genes using various vector systems, we believe that this novel system will become an innovative tool in the field of gene expression and gene therapy.

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  • Retroperitoneal bronchogenic cyst: a rare incidentaloma discovered in a juvenile hypertensive patient

    Terasaka T, Otsuka F, Ogura-Ochi K, Miyoshi T, Inagaki K, Kobayashi Y, Nasu Y, Makino H

    Hypertens Res   37 ( 6 )   595 - 597   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • A Rare Complication: Misdirection of an Indwelling Urethral Catheter into the Ureter 査読

    Tsutomu Ishikawa, Motoo Araki, Takeshi Hirata, Masami Watanabe, Shin Ebara, Toyohiko Watanabe, Yasutomo Nasu, Hiromi Kumon

    ACTA MEDICA OKAYAMA   68 ( 1 )   47 - 51   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We report 3 patients with the rare complication of an indwelling urethral catheter misdirected into the ureter. This is the largest series to date. Patients were referred to us for a variety of reasons following exchange of their chronic indwelling urinary catheters. CT in all cases demonstrated the urinary catheters residing in the left ureter. The ages of the patients were 37, 67 and 81 years old. All patients suffered from neurogenic bladder. Two patients were female, one was male, and 2 of the 3 had a sensory disorder inhibiting their pain response. The catheters were replaced with open-end Foley catheters. Extensive follow-up CT scans were obtained in one case, demonstrating improvement of hydronephrosis and no evidence of ureteral stenosis. Cystoscopy in this patient demonstrated normally positioned and functioning ureteral orifices. Although the placement of an indwelling urethral catheter is a comparatively safe procedure, one must keep in mind that this complication can occur, particularly in female patients with neurogenic bladder. CT without contrast is a noninvasive, definitive diagnostic tool.

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  • Anti-Cancer Effects of REIC/Dkk-3-encoding Adenoviral Vector for the Treatment of Non-small Cell Lung Cancer 査読

    Kazuhiko Shien, Norimitsu Tanaka, Masami Watanabe, Junichi Soh, Masakiyo Sakaguchi, Keitaro Matsuo, Hiromasa Yamamoto, Masashi Furukawa, Hiroaki Asano, Kazunori Tsukuda, Yasutomo Nasu, Nam-Ho Huh, Shinichiro Miyoshi, Hiromi Kumon, Shinichi Toyooka

    PLOS ONE   9 ( 2 )   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Objectives: REIC/Dkk-3 is down-regulated in a broad range of human cancer cells and is considered to function as a tumor suppressor. We previously reported that REIC/Dkk-3-expressing adenovirus vector (Ad-REIC) induced endoplasmic reticulum (ER) stress and cancer-specific apoptosis in human prostate cancer. In this study, we examined the therapeutic impact of Ad-REIC on non-small cell lung cancer (NSCLC).
    Materials and Methods: We examined the anti-tumor effect of Ad-REIC on 25 NSCLC cell lines in vitro and A549 cells in vivo. Two of these cell lines were artificially established as EGFR-tyrosine kinase inhibitor (TKI) resistant sublines.
    Results: Ad-REIC-treatment inhibited the cell viability by 40% or more in 13 (52%) of the 25 cell lines at multiplicity of infection (MOI) of 20 (20 MOI). These cell lines were regarded as being highly sensitive cells. The cell viability of a nonmalignant immortalized cell line, OUMS-24, was not inhibited at 200 MOI of Ad-REIC. The effects of Ad-REIC on EGFR-TKI resistant sublines were equivalent to those in the parental cell lines. Here, we demonstrated that Ad-REIC treatment activated c-Jun N-terminal kinase (JNK) in NSCLC cell lines, indicating the induction of ER stress with GRP78/BiP (GRP78) up-regulation and resulting in apoptosis. A single intratumoral injection of Ad-REIC significantly inhibited the tumorigenic growth of A549 cells in vivo. As predictive factors of sensitivity for Ad-REIC treatment in NSCLC, we examined the expression status of GRP78 and coxsackievirus and adenovirus receptor (CAR). We found that the combination of the GRP78 and CAR expressional statuses may be used as a predictive factor for Ad-REIC sensitivity in NSCLC cells.
    Conclusion: Ad-REIC induced JNK activation and subsequent apoptosis in NSCLC cells. Our study indicated that Ad-REIC has therapeutic potential against NSCLC and that the expression statuses of GRP78 and CAR may predict a potential therapeutic benefit of Ad-REIC.

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    その他リンク: http://orcid.org/0000-0003-1761-6314

  • Robot-Assisted Radical Prostatectomy: Modified Ultradissection Reduces pT2 Positive Surgical Margins on the Bladder Neck 査読

    Motoo Araki, Wooju Jeong, Sung Yul Park, Young Hoon Lee, Yasutomo Nasu, Hiromi Kumon, Sung Joon Hong, Koon Ho Rha

    ACTA MEDICA OKAYAMA   68 ( 1 )   35 - 41   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    The purpose of this study was to compare the positive surgical margin (PSM) rates of 2 techniques of robot-assisted radical prostatectomy (RARP) for pT2 (localized) prostate cancer. A retrospective analysis was conducted of 361 RARP cases, performed from May 2005 to September 2008 by a single surgeon (KHR) at our institution (Yonsei University College of Medicine). In the conventional technique, the bladder neck was transected first. In the modified ultradissection, the lateral border of the bladder neck was dissected and then the bladder neck was transected while the detrusor muscle of the bladder was well visualized. Perioperative characteristics and outcomes and PSM rates were analyzed retrospectively for pT2 patients (n = 217), focusing on a comparison of those undergoing conventional (n = 113) and modified ultradissection (n = 104) techniques. There was no difference between the conventional and modified ultradissection group in mean age, BMI, PSA, prostate volume, biopsy Gleason score, and D'Amico prognostic criteria distributions. The mean operative time was shorter (p < 0.001) and the estimated blood loss was less (p < 0.01) in the modified ultradissection group. The PSM rate for the bladder neck was significantly reduced by modified ultradissection, from 6.2% to 0% (p < 0.05). In conclusion, modified ultradissection reduces the PSM rate for the bladder neck.

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  • Testosterone replacement elevates the serum uric acid levels in patients with female to male gender identity disorder 査読

    Hiroaki Kurahashi, Masami Watanabe, Morito Sugimoto, Yuichi Ariyoshi, Sabina Mahmood, Motoo Araki, Kazushi Ishii, Yasutomo Nasu, Atsushi Nagai, Hiromi Kumon

    ENDOCRINE JOURNAL   60 ( 12 )   1321 - 1327   2013年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN ENDOCRINE SOC  

    Gender identity disorder (GID) results from a disagreement between a person's biological sex and the gender to which he or she identifies. With respect to the treatment of female to male GID, testosterone replacement therapy (TRT) is available. The uric acid (UA) level can be influenced by testosterone; however, the early effects and dose-dependency of TRT on the serum UA concentration have not been evaluated in this population. We herein conducted a dose-response analysis of TRT in 160 patients with female to male GID. The TRT consisted of three treatment groups who received intramuscular injections of testosterone enanthate: 125 mg every two weeks, 250 mg every three weeks and 250 mg every two weeks. Consequently, serum UA elevation was observed after three months of TRT and there was a tendency toward testosterone dose-dependency. The onset of hyperuricemia was more prevalent in the group who received the higher dose. We also demonstrated a positive correlation between increased levels of serum UA and serum creatinine. Since the level of serum creatinine represents an individual's muscle volume and the muscle is a major source of purine, which induces UA upregulation, the serum UA elevation observed during TRT is at least partially attributed to an increase in muscle mass. This is the first study showing an association between serum UA elevation and a TRT-induced increase in muscle mass. The current study provides important information regarding TRT for the follow-up and management of the serum UA levels in GID patients.

    DOI: 10.1507/endocrj.EJ13-0203

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  • Predictive factors for acute and late urinary toxicity after permanent interstitial brachytherapy in Japanese patients

    Tanimoto R, Bekku K, Katayama N, Kobayashi Y, Ebara S, Araki M, Takemoto M, Yanai H, Nasu Y, Kumon H

    Int J Urol   20 ( 8 )   812 - 817   2013年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Clinical significance of CD24 as a predictor of bladder cancer recurrence. 査読

    Chunxiao Liu, Shaobo Zheng, Haiyan Shen, Kai Xu, Jie Chen, Hulin Li, Yawen Xu, Abai Xu, Binshen Chen, Haruki Kaku, Yasutomo Nasu, Hiromi Kumon, Peng Huang, Masami Watanabe

    Oncology Letters   96 - 100   2013年7月

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    記述言語:英語  

    DOI: 10.3892/ol.2013.1357

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  • Dose-response analysis of testosterone replacement therapy in patients with female to male gender identity disorder

    Nakamura A, Watanabe M, Sugimoto M, Sako T, Mahmood S, Kaku H, Nasu Y, Ishii K, Nagai A, Kumon H

    Endocrine Journal   60 ( 3 )   275 - 281   2013年3月

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  • Could salvage surgery after chemotherapy have clinical impact on cancer survival of patients with metastatic urothelial carcinoma? 査読

    Kensuke Bekku, Takashi Saika, Yasuyuki Kobayashi, Ryo Kioshimoto, Taiki Kanbara, Yasutomo Nasu, Hiromi Kumon

    INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY   18 ( 1 )   110 - 115   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER JAPAN KK  

    The clinical impact of salvage surgery after chemotherapy on cancer survival of patients with metastatic urothelial carcinoma is controversial. We aimed to verify the clinical role of salvage surgery by analyzing the long-term outcome in patients with urothelial carcinoma treated by chemotherapy.
    Between 2003 and 2010 at a single institution, 31 of 47 patients (66%) with metastatic urothelial carcinoma showed objective responses (CR in 4, PR in 27) after multiple courses of cisplatin/gemcitabine/paclitaxel-based chemotherapy, and a cohort of patients with partial response (PR) were retrospectively enrolled. Twelve (10 male and 2 female, median age 64.0 years) of 27 patients with PR underwent salvage surgeries after the chemotherapy: metastatectomy of residual lesions (10 retroperitoneal lymph nodes, 2 lung), and 6 radical surgeries for primary lesions as well. Progression-free survival and overall patient survival rates were analyzed retrospectively and compared with those of patients without salvage surgery.
    All 12 patients achieved surgical CR. Pathological findings of metastatic lesions showed viable cancer cells in 3 patients. In univariate analysis, sole salvage surgery affected overall survival in 27 patients with PR to the chemotherapy (P = 0.0037). Progression-free survival and overall survival rates in patients with salvage surgery were better than those in 15 PR patients without the surgery (39.8 vs. 0%, and 71.6 vs. 12.1% at 3 years, P = 0.01032 and 0.01048; log-rank test).
    Salvage surgery for patients with residual tumor who achieve partial response to chemotherapy could have a possible impact on cancer survival.

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  • Cancer stem cell-like characteristics of a CD133+ subpopulation in the J82 human bladder cancer cell line. 査読

    Peng Huang, Masami Watanabe, Haruki Kaku, Hideo Ueki, Hirofumi Noguchi, Morito Sugimoto, Takeshi Hirata, Hiroshi Yamada, Kohji Takei, Shaobo Zheng, Kai Xu, Yasutomo Nasu, Yasuyuki Fujii, Chunxiao Liu, Hiromi Kumon

    Molecular and Clinical Oncology   180 - 184   2013年1月

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  • Preclinical biodistribution and safety study of reduced expression in immortalized cells/Dickkopf-3-encoding adenoviral vector for prostate cancer gene therapy 査読

    Morito Sugimoto, Masami Watanabe, Haruki Kaku, Shun-Ai Li, Hirofumi Noguchi, Hideo Ueki, Masakiyo Sakaguchi, Nam-Ho Huh, Yasutomo Nasu, Hiromi Kumon

    ONCOLOGY REPORTS   28 ( 5 )   1645 - 1652   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    The biodistribution and safety of adenoviral vectors encoding the human REIC/Dkk-3 tumor suppressor gene (Ad-REIC) were examined in this preclinical study for in situ prostate cancer gene therapy. First, the in vitro apoptotic effects of Ad-REIC in normal and cancer cells derived from the prostate and liver were examined. Significant apoptotic effects were observed at 100 MOI (multiplicity of infection) in prostate cancer cells (LNCaP, PC3) and hepatoma cells (HEP3B and HEPG2); however, no effects were seen in normal cells. To analyze the safety of intraprostatic Ad-REIC administration, the biodistribution and histology after Ad-REIC injection were evaluated in various organs of normal male C57BL6 mice. In a supporting study, vector dissemination following intravenous injection of Ad-REIC into tail veins was determined. To evaluate whether Ad-REIC was present in the collected tissue specimens, human REIC gene detection was performed using DNA-PCR. Intraprostatic treatment administered at lower doses showed vector biodistribution into the colon, urinary bladder and prostate. At higher doses, vector dissemination was observed in tissues more distant from the prostate, including the lung, thymus, heart, liver and adrenal gland. After intravenous injection a: Ad-REIC, dissemination was observed in the liver and spleen. These results indicate that the biodistribution of Ad-REIC is determined by the dose and route of administration. Although acute inflammatory effects were observed in the prostate after intraprostatic administration at higher doses, no abnormal histological findings were noted in the other tissues, including those of intravenously treated mice. Regarding the safety of Ad-REIC administration, no deaths and no signs of toxicity or unusual behavior were observed in the mice in any treatment group. Based on these preclinical experiments, adenovirus-mediated in situ REIC/Dkk-3 gene therapy is considered to be safe for use as a treatment for human prostate cancer.

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  • DIRECT THERAPEUTIC EFFECT OF REIC/DKK-3-ENCODING ADENOVIRAL VECTOR FOR NON-SMALL CELL LUNG CANCER 査読

    Yamamoto Hiromasa, Tanaka Norimitsu, Toyooka Shinichi, Watanabe Masami, Sakaguchi Masakiyo, Soh Junichi, Shien Kazuhiko, Furukawa Masashi, Asano Hiroaki, Tsukuda Kazunori, Nasu Yasutomo, Huh Nam-ho, Kumon Hiromi, Miyoshi Shinichirou

    JOURNAL OF THORACIC ONCOLOGY   7 ( 11 )   S463   2012年11月

  • Laparoscopic management of complicated urachal remnants in adults 査読

    Motoo Araki, Takashi Saika, Daiji Araki, Yasuyuki Kobayashi, Shinya Uehara, Toyohiko Watanabe, Kiyoshi Yamada, Yasutomo Nasu, Hiromi Kumon

    WORLD JOURNAL OF UROLOGY   30 ( 5 )   647 - 650   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Objectives The traditional surgical approach for removing a urachal remnant is via a large transverse or midline infraumbilical incision. We review our experience with laparoscopic urachal cyst excision and report the efficacy and outcomes of this approach as a less morbid, minimally invasive alternative.
    Methods Between August 2005 and March 2009, eight patients with a mean age of 26 years who had symptomatic urachal cysts underwent laparoscopic radical excision of the urachal remnant. Using three ports, the urachal remnant was dissected from the umbilicus to the bladder dome and then removed intact via the umbilicus. Umbilicoplasty was performed by a plastic surgeon. We retrospectively reviewed the perioperative records to assess morbidity and outcomes.
    Results All eight operations were completed successfully. No intraoperative or postoperative complications were reported at a mean follow-up of 3.2 years. Mean operative time was 147.5 min including umbilicoplasty. Pathological evaluation confirmed a benign urachal remnant in each case. There have been no recurrences of symptoms nor postoperative complications during follow-up. Mean time to full recovery, defined as return to normal life without pain, was 16 days. The patients with bladder cuff resection had a delayed full convalescence (25 vs. 13 days) due to a minimum degree of dysuria.
    Conclusion A laparoscopic approach with the removal of urachal remnants via the umbilicus appears to be a safe and effective alternative with better cosmesis when compared to an open approach.

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  • REIC/Dkk-3-encoding adenoviral vector as a potentially effective therapeutic agent for bladder cancer 査読

    Takeshi Hirata, Masami Watanabe, Haruki Kaku, Yasuyuki Kobayashi, Hiroshi Yamada, Masakiyo Sakaguchi, Kohji Takei, Nam-Ho Huh, Yasutomo Nasu, Hiromi Kumon

    INTERNATIONAL JOURNAL OF ONCOLOGY   41 ( 2 )   559 - 564   2012年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    Bladder cancer is one of the most common urogenital malignancies. The intravesical instillation of anticancer agents is an attractive strategy to treat a superficial lesion or floating/disseminated cancer cells after transurethral operation. An adenovirus carrying REIC/Dkk-3, a tumor suppressor gene (Ad-REIC), exhibits cancer-specific apoptotic effects in various types of cancer cells. The aim of the present study was to examine the potential of Ad-REIC as a therapeutic agent for bladder cancer. KK47 and RT4 human bladder cancer cells were sensitive to the Ad-REIC treatment for apoptosis induction, but some human bladder cancer cell lines (T24, J82 and TccSup) were resistant. Significant cell growth inhibition was observed when these resistant cancer cell lines were treated with Ad-REIC in a condition of floating cells, which is clinically observed after transurethral operation and becomes a cause of intravesical cancer dissemination. The therapeutic potential of Ad-REIC for the treatment of multidrug-resistant bladder cancer was investigated. The adriamycin-resistant KK47 bladder cancer cells (KK47/ADM), which also present multidrug resistance, showed induction of significant apoptosis following Ad-REIC treatment. The Ad-REIC treatment induced downregulation of P-glycoprotein in KK47/ADM cells and restored the sensitivity to doxorubicin (adriamycin). Ad-REIC suppressed P-glycoprotein expression in a c-Jun-NH2-kinase (JNK)-dependent manner. Therefore, the current study indicated two therapeutic aspects of the Ad-REIC agent against human bladder cancer cells, as an apoptosis inducer/cell growth inhibitor and as a sensitizer of chemotherapeutic agents in multidrug-resistant cancer cells. The intravesical instillation of Ad-REIC could be an attractive therapeutic method in human bladder cancer where the treatment of superficial lesions and floating/disseminated or multidrug-resistant cancer cells is necessary.

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  • A novel gene expression system for detecting viable bladder cancer cells 査読

    Hideo Ueki, Masami Watanabe, Haruki Kaku, Peng Huang, Shun-Ai Li, Kazuhiko Ochiai, Takeshi Hirata, Hirofumi Noguchi, Hiroshi Yamada, Kohji Takei, Yasutomo Nasu, Yuji Kashiwakura, Hiromi Kumon

    INTERNATIONAL JOURNAL OF ONCOLOGY   41 ( 1 )   135 - 140   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    A novel transcriptional system was developed that can robustly enhance cancer-specific gene expression. In the system, hTERT promoter-driven gene expression was enhanced by an advanced two-step transcriptional amplification (TSTA). This construct was used to develop a novel system for detection of bladder cancer cells. The current study evaluated the advanced TSTA system by examining the cancer-specific gene transcription in various bladder cancer cell lines. The system significantly enhanced cancer-specific luciferase gene expression in the bladder cancer cell lines in comparison to the previous expression system of one-step or conventional TSTA. The fold gain of the enhancement was significantly correlated to the telomerase activity of the cell lines. A green fluorescent protein (GFP) gene encoding plasmid vector was constructed where hTERT promoter-driving transcription is enhanced by the advanced TSTA to utilize the system for the imaging and detection of viable bladder cancer cells. The advanced TSTA-hTERT-GFP plasmid successfully induced cancer-specific gene expression, showing robust GFP expression in human bladder cancer cell lines, but no visible GFP expression in normal bladder urothelial cells. The control GFP plasm id with a CMV promoter yielded GFP expression in both normal bladder cells and cancer cells. The advanced TSTA-hTERT-GFP plasmid allowed selective visualization of viable human bladder cancer cells in mixed cell culture containing 10- and 100-fold more normal bladder urothelial cells. These findings indicate that the advanced TSTA-hTERT expressional system is a valuable tool for detecting viable bladder cancer cells. The current system can be applied for in vitro detection of bladder cancer cells in urine and other types of cancer cells disseminated in vivo.

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  • The clinical impact of pathological review on selection the treatment modality for localized prostate cancer in candidates for brachytherapy monotherapy 査読

    Ryo Kishimoto, Takashi Saika, Kensuke Bekku, Hiroyuki Nose, Fernando Abarzua, Yasuyuki Kobayashi, Motoo Araki, Hiroyuki Yanai, Yasutomo Nasu, Hiromi Kumon

    WORLD JOURNAL OF UROLOGY   30 ( 3 )   375 - 378   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    To evaluate the impact of pathological review by pathologist with genitourinary expertise (PGU) on treatment modality of localized prostate cancer, we analyzed Gleason grade (GG) migration and the final treatment decision in a cohort of patients designated for permanent prostate brachytherapy (PPB).
    From February 2005 to July 2010, a total of 247 patients with localized prostate cancer diagnosed by local community hospitals were referred to our hospital for PPB monotheray. All pathologic slides of prostate biopsies were reviewed by a single PGU. Patients ultimately selected their treatment modality from our recommendations based on the review. Indication for PPB monotherapy was the NCCN classification of patients as good or intermediate risk. In addition, patient with Primary GG 4 was regarded as unadapted case.
    Six cases were reinterpreted as no cancer (2.4%). GG change occurred in 94 cases (38.1%) of which 77 (81.9%) were upgraded and 17 (18.1%) downgraded. Of the total 247 patients, 86 (34.8%) changed therapies and 30 (12.1%) did so based on the pathologic slide review.
    Pathological review of biopsy specimens is mandatory for the determination of treatment modality especially in candidates for monotherapy of permanent prostate brachytherapy.

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  • Implications of transcriptional factor, OCT-4, in human bladder malignancy and tumor recurrence 査読

    Peng Huang, Jie Chen, Lei Wang, Yanqun Na, Haruki Kaku, Hideo Ueki, Katsumi Sasaki, Ken Yamaguchi, Kai Zhang, Takashi Saika, Yasutomo Nasu, Masami Watanabe, Hiromi Kumon

    MEDICAL ONCOLOGY   29 ( 2 )   829 - 834   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:HUMANA PRESS INC  

    OCT-4, which is also known as POU5f1, is a key regulator of self-renewal in embryonic stem cells. The new cancer stem cell concept proposes that the expression of such genes is potentially correlated with tumorigenesis and can affect some aspects of the cancer behavior, such as recurrence or metastasis. This study investigated the association between OCT-4 expression in cancer tissues obtained by transurethral surgery and the clinical data to clarify the involvement of OCT-4 in human bladder malignancy. Immunohistochemical analysis demonstrated that a positive rate of OCT-4 expression was significantly associated with the higher-grade cancer (G2 and G3) in comparison with that of the lower grade (G1). In addition, positive OCT-4 expression was significantly associated with the intra-bladder tumor recurrence after the operation. The staining intensity of OCT-4 expression was also correlated with tumor recurrence. These data indicate that positive OCT-4 expression may be involved in the development of high-grade bladder cancer and with the bladder cancer recurrence. This is the first study showing a correlation between the expression of OCT-4 and bladder cancer recurrence. OCT-4 may be a valuable clinical marker for the progression of bladder cancer and may be an attractive therapeutic target for the development of new medicines for the treatment of malignancy.

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  • Ureteroscopic Management of Chronic Unilateral Hematuria: A Single-Center Experience over 22 Years 査読

    Motoo Araki, Shinya Uehara, Katsumi Sasaki, Koichi Monden, Masaya Tsugawa, Toyohiko Watanabe, Manoji Monga, Yasutomo Nasu, Hiromi Kumon

    PLOS ONE   7 ( 6 )   e36729   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Objective: To analyze the short and long term safety and efficacy of ureteroscopic evaluation and management of chronic unilateral hematuria.
    Methods: We retrospectively reviewed patients with chronic unilateral hematuria from 1987 to 2008. The distal to middle ureter was evaluated with a semi-rigid ureteroscope without a guidewire. Subsequently, the flexible ureteroscope was advanced into the upper ureter to the renal pelvis using a low-pressure automated irrigant system (Uromat (TM)). Lesions identified ureteroscopically were treated with diathermy fulguration.
    Results: One hundred and four (56 male, 48 female) patients were identified, with a median age of 37 (14-80) years and median follow-up of 139 (34-277) months. The median preoperative duration of gross hematuria was 5 (1-144) months. Endoscopic findings included 61 (56%) minute venous rupture (MVR; a venous bleeding without clear abnormalities), 21 (20%) hemangioma (vascular tumor-like structure), 3 (3%) varix (tortuous vein), 1 (1%) calculus and 18 (17%) no lesions. The incidence of "no lesions" was less in the recent 12 years (9%) than the first 10 years (27%), while the incidence of MVR increased from 40 to 66% (p<0.05). All patients were treated endoscopically. Immediate success rate was 96% (100% in the recent 12 years). Long-term recurrent gross hematuria rate was 7%. Six resolved spontaneously and only 1 required ureteroscopy, revealing a different bleeding site.
    Conclusion: Ureteroscopy and diathermy fulguration is highly useful for evaluation and treatment of chronic unilateral hematuria. Sophisticated technique and improved instrumentation contributes to a better outcome.

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  • DNA methylation status of REIC/Dkk-3 gene in human malignancies 査読

    Tatsuro Hayashi, Hiroaki Asano, Shinichi Toyooka, Kazunori Tsukuda, Junichi Soh, Tadahiko Shien, Naruto Taira, Yuho Maki, Norimitsu Tanaka, Hiroyoshi Doihara, Yasutomo Nasu, Nam-ho Huh, Shinichiro Miyoshi

    JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY   138 ( 5 )   799 - 809   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    The REIC (reduced expression in immortalized cells)/Dkk-3 is down-regulated in various cancers and considered to be a tumor suppressor gene. REIC/Dkk-3 mRNA has two isoforms (type-a,b). REIC type-a mRNA has shown to be a major transcript in various cancer cells, and its promoter activity was much stronger than that of type-b. In this study, we examined the methylation status of REIC/Dkk-3 type-a in a broad range of human malignancies.
    We examined REIC/Dkk-3 type-a methylation in breast cancers, non-small-cell lung cancers, gastric cancers, colorectal cancers, and malignant pleural mesotheliomas using a quantitative combined bisulfite restriction analysis assay and bisulfate sequencing. REIC/Dkk-3 type-a and type-b expression was examined using reverse transcriptional PCR. The relationships between the methylation and clinicopathological factors were analyzed.
    The rate of REIC/Dkk-3 type-a methylation ranged from 26.2 to 50.0% in the various primary tumors that were examined. REIC/Dkk-3 type-a methylation in breast cancer cells was significantly heavier than that in the other cell lines that we tested. REIC/Dkk-3 type-a methylation was inversely correlated with REIC/Dkk-3 type-a expression. There was a correlation between REIC/Dkk-3 type-a and type-b mRNA expression. REIC/Dkk-3 type-a expression was restored in MDA-MB-231 cells using 5-aza-2'-deoxycytidine treatment. We found that estrogen receptor-positive breast cancers were significantly more common among the methylated group than among the non-methylated group.
    REIC/Dkk-3 type-a methylation was frequently detected in a broad range of cancers and appeared to play a key role in silencing REIC/Dkk-3 type-a expression in these malignancies.

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  • Preoperative positive urine cytology is a risk factor for subsequent development of bladder cancer after nephroureterectomy in patients with upper urinary tract urothelial carcinoma 査読

    Yasuyuki Kobayashi, Takashi Saika, Yoshiyuki Miyaji, Michinao Saegusa, Ryoji Arata, Naoki Akebi, Tadasu Takenaka, Daisuke Manabe, Yasutomo Nasu, Hiromi Kumon

    WORLD JOURNAL OF UROLOGY   30 ( 2 )   271 - 275   2012年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    To determine the independent risk factors of bladder recurrence in patients with upper urinary tract urothelial carcinoma (UUT-UC).
    A total of 364 patients underwent nephroureterectomy (NUx) for UUT-UC between January 2005 and April 2009 in Okayama University and 17 affiliated hospitals. Patients with concomitant bladder cancer were excluded from the analysis. The clinicopathologic data for the remaining 288 patients with UUT-UC were retrospectively reviewed. Median follow-up after NUx was 20.2 months. The following variables were evaluated for any association with bladder recurrence: sex, age, tumor stage, tumor grade, venous invasion, lymphatic invasion, tumor location, multifocality, surgical modalities, time of ligation of the ureter, and preoperative urine cytology. The significance of each variable was tested univariately using the log-rank test. The simultaneous effects of multiple risk factors were estimated by multiple regression analysis using the Cox proportional hazards model.
    Bladder recurrence occurred in 103 patients (35.8%). Median time to first bladder recurrence was 6.9 months. Significant risk factors for bladder recurrences on univariate analysis were tumor location (P = 0.046) and preoperative positive urine cytology (P < 0.001). Multivariate analysis revealed that preoperative urine cytology positive was significant for bladder recurrence (HR: 1.977; 95% CI: 1.310-2.983, P = 0.001).
    Risk factor for subsequent development of bladder cancer after NUx was preoperative positive urine cytology.

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  • Partial sensitization of human bladder cancer cells to a gene-therapeutic adenovirus carrying REIC/Dkk-3 by downregulation of BRPK/PINK1 査読

    Yu Jin, Hitoshi Murata, Masakiyo Sakaguchi, Ken Kataoka, Masami Watanabe, Yasutomo Nasu, Hiromi Kumon, Nam-Ho Huh

    ONCOLOGY REPORTS   27 ( 3 )   695 - 699   2012年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    REIC/Dkk-3 is a tumor suppressor gene that was first identified as a gene downregulated in association with immortalization of normal human fibroblasts. We have demonstrated that an adenovirus carrying REIC/Dkk-3 (Ad-REIC) showed a tumor-specific killing effect on a wide range of cancers. However, some human cancers, bladder cancers in particular, are resistant to Ad-REIC. In this study, we investigated the combination effect of downregulation of BRPK/PINK1 (PINK1) and Ad-REIC on bladder cancer cells. Five bladder cancer cell lines among six cell lines examined were resistant to Ad-REIC. Among the cell lines, the resistance of two cell lines was probably due to low infection efficiency of the adenovirus. PINK1-specific siRNA remarkably downregulated Bcl-x(L) and TRAP1 proteins and upregulated BAX protein expression. Finally, downregulation of PINK1 partially sensitized the other three cell lines that were resistant to Ad-REIC. This sensitization was associated with increasing production of reactive oxygen species (ROS). These results indicate that PINK1 is one of the key molecules for the mitochondrial protection system and that PINK1 can be a new target molecule to sensitize bladder cancer cells that are resistant to Ad-REIC.

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  • Preclinical Safety and Efficacy of in Situ REIC/Dkk-3 Gene Therapy for Prostate Cancer 査読

    Keiichiro Kawauchi, Masami Watanabe, Haruki Kaku, Peng Huang, Kasumi Sasaki, Masakiyo Sakaguchi, Kazuhiko Ochiai, Nam-ho Huh, Yasutomo Nasu, Hiromi Kumon

    ACTA MEDICA OKAYAMA   66 ( 1 )   7 - 16   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    The preclinical safety and therapeutic efficacy of adenoviral vectors that express the REIC/Dkk-3 tumor suppressor gene (Ad-REIC) was examined for use in prostate cancer gene therapy. The Ad-human (h) and mouse (m) REIC were previously demonstrated to induce strong anti-cancer effects in vitro and in vivo, and we herein report the results of two in vivo studies. First, intra-tumor Ad-hREIC administration was examined for toxicity and therapeutic effects in a subcutaneous tumor model using the PC3 prostate cancer cell line. Second, intra-prostatic Ad-mREIC administration was tested for toxicity in normal mice. The whole-body and spleen weights, hematological and serum chemistry parameters, and histological evaluation of tissues from throughout the body were analyzed. Both experiments indicated that there was no significant difference in the examined parameters between the Ad-REIC-treated group and the control (PBS- or Ad-LacZ-treated) group. In the in vitro analysis using PC3 cells, a significant apoptotic effect was observed after Ad-hREIC treatment. Confirming this observation, the robust anti-tumor efficacy of Ad-hREIC was demonstrated in the in vivo subcutaneous prostate cancer model. Based on the results of these preclinical experiments, we consider the adenovirus-mediated REIC/Dkk-3 in situ gene therapy to be safe and useful for the clinical treatment of prostate cancer.

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  • Primary Gleason grade 4 impact on biochemical recurrence after permanent interstitial brachytherapy in Japanese patients with low- or intermediate-risk prostate cancer. 査読

    Uesugi T, Saika T, Edamura K, Nose H, Kobuke M, Ebara S, Abarzua F, Katayama N, Yanai H, Nasu Y, Kumon H

    International journal of radiation oncology, biology, physics   82 ( 2 )   e219 - 23   2012年2月

  • URETEROSCOPIC MANAGEMENT FOR UPPER TRACT UROTHELIAL CARCINOMA: LONG-TERM SINGLE INSTITUTION EXPERIENCE 査読

    Araki Motoo, Uehara Shinya, Watanabe Toyohiko, Koichi Monden, Masaya Tsugawa, Yasuyuki Kobayashi, Shin Ebara, Saika Takashi, Yasutomo Nasu, Hiromi Kumon

    JOURNAL OF ENDOUROLOGY   25   A177 - A177   2011年11月

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    記述言語:英語   出版者・発行元:MARY ANN LIEBERT INC  

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  • Advanced two-step transcriptional amplification as a novel method for cancer-specific gene expression and imaging 査読

    Masami Watanabe, Hideo Ueki, Kazuhiko Ochiai, Peng Huang, Yasuyuki Kobayashi, Yasutomo Nasu, Katsumi Sasaki, Haruki Kaku, Yuji Kashiwakura, Hiromi Kumon

    ONCOLOGY REPORTS   26 ( 4 )   769 - 775   2011年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    The two-step transcriptional amplification (TSTA) system was previously reported to enhance the tissue-specific gene expression driven by weak promoters, but the enhancement of the gene expression is limited to use in in vitro and in vivo experimental situations. To achieve robust tissue-specific gene expression using the TSTA system, we developed an advanced TSTA system which includes polyglutamines and rat glucocorticoid receptor sequences between the GAL4 and VP16 sequences in the region of the first step of transcription. We evaluated the advanced TSTA system as a method to enhance the human telomerase reverse transcriptase (hTERT) promoter-driving cancer-specific transcription in various cancer cell lines. As a result, the advanced TSTA enhanced cancer-specific luciferase gene expression in all of the examined cancer cell lines, when compared with both the one-step and conventional TSTA systems (an similar to 6- and similar to 17-fold enhancement, respectively). Notably, the enhancement of the hTERT driven expression by the conventional TSTA system was modest and even inferior to the one-step system in several cancer cell lines. We then constructed a luciferase gene encoding the adeno-associated virus vector in which the hTERT promoter-mediated expression was driven by the advanced TSTA or control systems. In an orthotopic liver tumor model, mice were treated with the vector via tail vein injection. An optical imaging device was used to visualize the in vivo luciferase expression in the orthotopic tumor. The advanced TSTA system significantly enhanced the luciferase expression compared with the one-step and conventional TSTA systems (18.0+/-1.0- and 15.9+/-0.85-fold gain, respectively). Therefore, the advanced TSTA system significantly improves hTERT-dependent cancer-specific gene expression both in vitro and in vivo when compared with the previous systems. Since the advanced TSTA method can also be applied to other site-specific gene expression systems using tissue-specific promoters, this approach is expected to become a valuable tool enabling in vivo site-specific targeting in the field of gene therapy and molecular imaging.

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  • Single Nucleotide Polymorphism WRN Leu1074Phe Is Associated with Prostate Cancer Susceptibility in Chinese Subjects 査読

    Lei Wang, Haruki Kaku, Peng Huang, Kexin Xu, Kai Yang, Jiheng Zhang, Ming Li, Liping Xie, Xiaofeng Wang, Akiko Sakai, Masami Watanabe, Yasutomo Nasu, Kenji Shimizu, Hiromi Kumon, Yanqun Na

    ACTA MEDICA OKAYAMA   65 ( 5 )   315 - 323   2011年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    Deficiencies in the human DNA repair gene WRN are the cause of Werner syndrome, a rare autosomal recessive disorder characterized by premature aging and a predisposition to cancer. This study evaluated the association of WRN Leu1074Phe (rs1801195), a common missense single nucleotide polymorphism in WRN, with prostate cancer susceptibility in Chinese subjects. One hundred and forty-seven prostate cancer patients and 111 male cancer-free control subjects from 3 university hospitals in China were included. Blood samples were obtained from each subject, and the single nucleotide polymorphism WRN Leu1074Phe was genotyped by using a Snapshot assay. The results showed that WRN Leu1074Phe was associated with the risk of prostate cancer in Chinese men and that the TG/GG genotype displayed a decreased prevalence of prostate cancer compared with the TT genotype (OR = 0.58, 95% CI: 0.35-0.97, p = 0.039). Through stratified analysis, more significant associations were revealed for the TG/GG genotype in the subgroup with diagnosis age <= 72 yr (OR = 0.27, 95% CI: 0.12-0.61, p = 0.002) and in patients with localized diseases (OR = 0.36, 95% CI: 0.19-0.70, p = 0.003). However, no statistically significant difference was found in the subgroup with age > 72 yr or in patients with advanced diseases. We concluded that the genetic variant Leu1074Phe in the DNA repair gene WRN might play a role in the risk of prostate cancer in Chinese subjects.

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  • T2*-weighted image/T2-weighted image fusion in postimplant dosimetry of prostate brachytherapy

    Katayama N, Takemoto M, Yoshio K, Katsui K, Uesugi T, Nasu Y, Matsushita T, Kaji M, Kumon H, Kanazawa S

    J Radiat Res   52 ( 5 )   680 - 684   2011年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1 査読

    Kazuhiko Ochiai, Masami Watanabe, Hideo Ueki, Peng Huang, Yasuyuki Fujii, Yasutomo Nasu, Hirofumi Noguchi, Takeshi Hirata, Masakiyo Sakaguchi, Nam-ho Huh, Yuji Kashiwakura, Haruki Kaku, Hiromi Kumon

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   412 ( 2 )   391 - 395   2011年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    REIC/Dkk-3 is a member of the Dickkopf family proteins known as Wnt-antagonists, and REIC/Dkk-3 expression is downregulated in a broad range of cancer types. REIC/Dkk-3 acts as a tumor suppressor in multiple cancer cell lines by inducing apoptosis through endoplasmic reticulum (ER) stress signaling. However, the intracellular interaction partners of REIC/Dkk-3 have not been fully elucidated. By employing yeast two-hybrid screening, we identified the human dynein light chain, Tctex-1, as a novel interaction partner of REIC/Dkk-3. We further disclosed that the interaction involves the 136-157 amino acid region of REIC/Dkk-3 by using the mammalian two-hybrid system. Interestingly, this binding region of REIC/Dkk-3 with Tctex-1 contains an amino acid sequence motif [-(E) under bar -X-(G) under bar-(R) under bar-(R) under bar -X-(H) under bar-] which was previously reported as the Tctex-1 binding domain of dynein intermediate chain (DIC). Immunocytochemistry demonstrated that both REIC/Dkk-3 and Tctex-1 were localized around the ER of human fibroblasts, and the similar distribution pattern of the proteins suggests that their interaction occurs around the ER. This is the first study showing the interaction of a Dickkopf family protein with a dynein motor complex protein. The link between REIC/Dkk-3 and Tctex-1 may be of significance for understanding the molecular functions of the proteins in ER stress signaling and intracellular dynein motor dynamics, respectively. (C) 2011 Elsevier Inc. All rights reserved.

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  • Epigenetic Silencing of MicroRNA-34b/c Plays an Important Role in the Pathogenesis of Malignant Pleural Mesothelioma 査読

    Takafumi Kubo, Shinichi Toyooka, Kazunori Tsukuda, Masakiyo Sakaguchi, Takuya Fukazawa, Junichi Soh, Hiroaki Asano, Tsuyoshi Ueno, Takayuki Muraoka, Hiromasa Yamamoto, Yasutomo Nasu, Takumi Kishimoto, Harvey I. Pass, Hideki Matsui, Nam-ho Huh, Shinichiro Miyoshi

    CLINICAL CANCER RESEARCH   17 ( 15 )   4965 - 4974   2011年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC CANCER RESEARCH  

    Purpose: Malignant pleural mesothelioma (MPM) is an aggressive tumor with a dismal prognosis. Unlike other malignancies, TP53 mutations are rare in MPM. Recent studies have showed that altered expression of microRNA (miRNA) is observed in human malignant tumors. In this study, we investigated the alterations of miR-34s, a direct transcriptional target of TP53, and the role of miR-34s on the pathogenesis of MPM.
    Experimental Design: Aberrant methylation and expression of miR-34s were examined in MPM cell lines and tumors. miR-34b/c was transfected to MPM cells to estimate the protein expression, cell proliferation, invasion, and cell cycle.
    Results: Aberrant methylation was present in 2 (33.3%) of 6 MPM cell lines and 13 (27.7%) of 47 tumors in miR-34a and in all 6 MPM cell lines (100%) and 40 (85.1%) of 47 tumors in miR-34b/c. Expression of miR-34a and 34b/c in all methylated cell lines was reduced and restored with 5-aza-2'-deoxycytidine treatment. Because epigenetic silencing was the major event in miR-34b/c, we investigated the functional role of miR-34b/c in MPM. miR-34b/c-transfected MPM cells with physiologic miR-34b/c expression exhibited antiproliferation with G(1) cell cycle arrest and suppression of migration, invasion, and motility. The forced overexpression of miR-34b/c, but not p53, showed a significant antitumor effect with the induction of apoptosis in MPM cells.
    Conclusions: We show that the epigenetic silencing of miR-34b/c by methylation is a crucial alteration and plays an important role in the tumorigenesis of MPM, suggesting potential therapeutic options for MPM. Clin Cancer Res; 17(15); 4965-74. (C)2011 AACR.

    DOI: 10.1158/1078-0432.CCR-10-3040

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  • Nerve growth factor level in the prostatic fluid of patients with chronic prostatitis/chronic pelvic pain syndrome is correlated with symptom severity and response to treatment 査読

    Toyohiko Watanabe, Miayabi Inoue, Katsumi Sasaki, Motoo Araki, Shinya Uehara, Koichi Monden, Takashi Saika, Yasutomo Nasu, Hiromi Kumon, Michael B. Chancellor

    BJU INTERNATIONAL   108 ( 2 )   248 - 251   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    OBJECTIVE
    To explore whether levels of nerve growth factor (NGF) in expressed prostatic secretions (EPS) are correlated with symptom severity in chronic prostatitis (CP) and chronic pelvic pain syndrome (CPPS).
    PATIENTS AND METHODS
    All patients with CP/CPPS underwent a complete history and physical examination, and were scored according to the National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI).
    Expressed prostatic secretion samples from 20 patients with CP/CPPS and from four asymptomatic control patients were collected and frozen, and NGF levels in EPS were measured by enzyme-linked immunosorbent assay.
    Patients were asked to complete NIH-CPSI questionnaires at baseline and 8 weeks after treatment and patients with at least a 25% decrease in total NIH-CPSI score from the baseline values were classified as responders to treatment.
    RESULTS
    The mean (+/-SD) NGF levels in EPS of patients with CP/CPPS and asymptomatic control patients were 7409 (+/-3788) pg/mL and 4174 (+/-1349) pg/mL, respectively. The NGF level in patients with CP/CPPS correlated directly with pain severity (P = 0.014, r = 0.541).
    There were no significant differences between NGF levels in EPS before and after treatment. However, successful treatment significantly decreased NGF levels in responders (P = 0.001).
    CONCLUSION
    Nerve growth factor might contribute to the pathophysiology of CP/CPPS as changes in NGF level in EPS occurred in proportion to pain severity. Therefore, these results suggest that NGF could be used as a new biomarker to evaluate the symptoms of CP/CPPS and the effects of treatment.

    DOI: 10.1111/j.1464-410X.2010.09716.x

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  • Maintenance therapy with bacillus Calmette-Guerin Connaught strain clearly prolongs recurrence-free survival following transurethral resection of bladder tumour for non-muscle-invasive bladder cancer

    Hinotsu S, Akaza H, Naito S, Ozono S, Sumiyoshi Y, Noguchi S, Yamaguchi A, Nagamori S, Terai A, Nasu Y, Kume H, Tomita Y, Tanaka Y, Samma S, Uemura H, Koga H, Tsushima T

    BJU International   108 ( 2 )   187 - 195   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Nerve growth factor level in the prostatic fluid of patients with chronic prostatitis/chronic pelvic pain syndrome is correlated with symptom severity and response to treatment

    Watanabe T, Inoue M, Sasaki K, Araki M, Uehara S, Monden K, Saika T, Nasu Y, Kumon H, Chancellor MB

    JU   185 ( 4 )   1301 - 1302   2011年7月

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    記述言語:英語  

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  • [REIC/Dkk-3 gene therapy]. 査読

    Masami Watanabe, Haruki Kaku, Peng Huang, Hiromi Kumon, Yasutomo Nasu

    Nihon rinsho. Japanese journal of clinical medicine   69 Suppl 5   559 - 63   2011年6月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • Unexpected occurrence of adrenal Cushing's syndrome in a patient with systemic lupus erythematosus 査読

    Akihiro Katayama, Fumio Otsuka, Katsuyuki Tanabe, Naoko Tsukamoto, Ryutaro Yamanaka, Yoshinori Matsumoto, Yasutomo Nasu, Hirofumi Makino

    HYPERTENSION RESEARCH   34 ( 5 )   662 - 663   2011年5月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    DOI: 10.1038/hr.2011.13

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  • Intraperitoneal administration of an adenovirus vector carrying REIC/Dkk-3 suppresses peritoneal dissemination of scirrhous gastric carcinoma 査読

    Swe Swe Than, Ken Kataoka, Masakiyo Sakaguchi, Hitoshi Murata, Fernando Abarzua, Chika Taketa, Gang Du, Masakazu Yashiro, Kazuyoshi Yanagihara, Yasutomo Nasu, Hiromi Kumon, Nam-Ho Huh

    ONCOLOGY REPORTS   25 ( 4 )   989 - 995   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    Expression levels of the novel tumor suppressor gene REIC/Dkk-3 are reduced in many human cancers. We have previously showed that an adenovirus vector carrying REIC/Dkk-3 (Ad-REIC) induced apoptosis of cancer cells selectively and exerted bystander antitumor effects via ER stress. We examined possible effects of Ad-REIC in a peritoneal dissemination model of scirrhous gastric carcinoma (SGC). Among various types of gastric cancer, SGC continues to be associated with the worst prognosis due to a high incidence of metastases in the peritoneal cavity. We found that a single intraperitoneal injection of Ad-REIC suppressed tumor dissemination and disease progression. Immunomodulation by Ad-REIC led to recruitment of natural killer cells inside tumor nodules. We conclude that Ad-REIC gene therapy may be a potential tool in combinatorial approaches to achieve curative effects in SGC.

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  • CHANGES IN CLITORIAL BLOOD FLOW FOLLOWING SURGERY WITH TENSION-FREE VAGINAL MESH FOR PELVIC ORGAN PROLAPSE 査読

    Toyohiko Watanabe, Miyabi Inoue, Ayano Ishii, Toyoko Yamato, Motoo Araki, Shinya Uehara, Takashi Saika, Yasutomo Nasu, Hiromi Kumon

    JOURNAL OF UROLOGY   185 ( 4 )   E685 - E685   2011年4月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    DOI: 10.1016/j.juro.2011.02.1894

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  • PRIMARY GLEASON GRADE 4 IMPACTS ON BIOCHEMICAL RECURRENCE AFTER PERMANENT INTERSTITIAL BRACHYTHERAPY IN JAPANESE PATIENTS WITH LOW- OR INTERMEDIATE-RISK PROSTATE CANCER 査読

    Takashi Saika, Tatsuya Uesugi, Kohei Edamura, Hiroyuki Nose, Makoto Kobuke, Shin Ebara, Norihisa Katayama, Hiroyuki Yanai, Yasutomo Nasu, Hiromi Kumon

    JOURNAL OF UROLOGY   185 ( 4 )   E523 - E523   2011年4月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    DOI: 10.1016/j.juro.2011.02.1008

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  • Prognostic Factors Influencing Survival after Nephroureterectomy for Transitional Cell Carcinoma of the Upper Urinary Tract 査読

    Yasuyuki Kobayashi, Takashi Saika, Daisuke Manabe, Yasutomo Nasu, Hiromi Kumon

    ACTA MEDICA OKAYAMA   65 ( 2 )   A27 - A31   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We analyzed the prognostic factors influencing survival after surgeries for upper urinary tract urothelial carcinoma (UUT-UC) with longer follow-up periods than in previous studies. Between January 2000 and December 2004, 386 patients underwent nephroureterectomy for UUT-UC. The data for the 221 patients with UUT-UC were retrospectively reviewed. Nine variables were evaluated for association with the survival outcomes of disease-specific survival. The prognostic significance was tested univariately with the log-rank test. The simultaneous effects of multiple prognostic factors were estimated by multiple regression analysis using the Cox proportional hazards model. The median follow-up was 38.4 months. The 5-year over all survival was 62.3%. Significant prognostic factors for disease-specific survival rate on univariate analysis were pathological stage (p < 0.0001), tumor grade (p = 0.0324), and venous invasion (p < 0.0001). Multivariate analysis revealed that only venous invasion was significant for disease-specific survival rate (p = 0.0205). Venous invasion was the only independent prognostic factor in pathologically localized UUT-UC.

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  • URETEROSCOPIC EVALUATION AND TREATMENT OF CHRONIC UNILATERAL HEMATURIA: A 24-YEAR EXPERIENCE 査読

    Motoo Araki, Shinya Uehara, Koichi Monden, Toyohiko Watanabe, Takashi Saika, Yasutomo Nasu, Hiromi Kumon

    JOURNAL OF UROLOGY   185 ( 4 )   E556 - E557   2011年4月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    DOI: 10.1016/j.juro.2011.02.1240

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  • Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development

    Kai Zhang, Masami Watanabe, Yuji Kashiwakura, Shun-Al Li, Kohei Edamura, Peng Huang, Ken Yamaguchi, Yasutomo Nasu, Yasuyuki Kobayashi, Masakiyo Sakaguchi, Kazuhiko Ochiai, Hiroshi Yamada, Kohji Takei, Hideo Ueki, Nam-Ho Huh, Ming Li, Haruki Kaku, Yanqun Na, Hiromi Kumon

    INTERNATIONAL JOURNAL OF ONCOLOGY   37 ( 6 )   1495 - 1501   2010年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    The tumor suppressor REIC/Dkk-3 is a secretory protein which was originally identified to be downregulated in human immortalized cells In the present study, we investigated the expression pattern of REIC/Dkk-3 in various cell types to characterize its physiological functions We first examined the expression level of REIC/Dkk-3 in a broad range of cancer cell types and confirmed that it was significantly downregulated in all of the cell types We also examined the tissue distribution pattern in a variety of normal mouse organs Ubiquitous REIC/Dkk-3 protein expression was observed in the organs The expression was abundant in the liver, heart and brain tissue, but was absent in the spleen and peripheral blood mononuclear cells The immunohistochemical analyses revealed that the subcellular localization of REIC/Dkk-3 had a punctate pattern around the nucleus, indicating its association with secretory vesicles In cancer cells stably transfected with REIC/Dkk-3 the protein was predominantly localized to the endoplasmic reticulum (ER) under observation with confocal microscopy Because REIC/ Dkk-3 was found to be abundantly expressed in the acinar epithelial cells of the mouse prostate, we analyzed the effects of recombinant REIC/Dkk-3 protein on the acinar morphogenesis of RWPE-1 cells, which are derived from human normal prostate epithelium Statistically significant acinar growth was observed in the culture condition with 10 mu g/m1 REIC/Dkk-3 protein, implicating the soluble form m prostatic acinar development Current results suggest that REIC/Dkk-3 may play a role in regulating the morphological process of normal tissue architecture through an autocrine and/or paracrine manner

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  • Indications for Ureteropyeloscopy Based on Radiographic Findings and Urine Cytology in Detection of Upper Urinary Tract Carcinoma 査読

    Akira Takao, Takashi Saika, Shinya Uehara, Koichi Monden, Fernando Abarzua, Yasutomo Nasu, Hiromi Kumon

    JAPANESE JOURNAL OF CLINICAL ONCOLOGY   40 ( 11 )   1087 - 1091   2010年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    To verify the indication of diagnostic ureteropyeloscopy based on clinical features for upper urinary tract urothelial cancer with over 100 patients and over a 10-year series.
    From January 1997 to December 2008, consecutive 129 units in 124 patients underwent ureteropyeloscopy to obtain a definitive diagnosis of upper urinary tract cancer or to rule out a malignancy. Patients were divided into four subgroups based on voided urine cytology and preoperative radiographic findings: group A (n = 8), positive urine cytology and positive radiographic findings; group B (n = 4), positive cytology and negative radiographic findings; group C (n = 55), negative cytology and positive radiographic findings and group D (n = 62), gross hematuria originating from the upper urinary tract with negative cytology and negative radiographic findings. Ureteropyeloscopic findings were compared with radiographic and cytological results. Adverse effects were also investigated.
    In group A, all patients had confirmed cancer. In group B, one revealed small cancer and the remaining three confirmed carcinoma in situ by biopsy with ureteropyeloscopy. In groups C and D, 33 patients (60%) and four (6.5%) revealed cancer. Seventy-eight patients out of 80 (97.5%) in groups C and D were confirmed to have benign disease. No patient was found with malignancy during follow up after negative finding of ureteropyeloscopy.
    Ureteropyeloscopy can help in detecting upper urinary tract cancer or to rule out malignancy for patients with negative voiding cytology. However, ureteropyeloscopy is redundant for patients with positive radiographic findings and positive voiding cytology.

    DOI: 10.1093/jjco/hyq096

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  • Bladder cancer working group report

    Kakehi Y, Hirao Y, Kim W-J, Ozono S, Masumori N, Miyanaga N, Nasu Y, Yokomizo A

    Jpn J Clin Oncol   40 ( Suppl1 )   57 - 64   2010年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Hepatocyte growth factor activator inhibitors(HAI-1 and HAI-2) are potential targets in uterine leiomyosarcoma

    Nakamura K, Abarzua F, Hongo A, Kodama J, Nasu Y, Kumon H, Hiramatsu Y

    Int J Oncol   37 ( 3 )   605 - 614   2010年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Two instillations of epirubicin as prophylaxis for recurrence after transurethral resection of Ta and T1 transitional cell bladder cancer: a prospective, randomized controlled study 査読

    Takashi Saika, Tomoyasu Tsushima, Yasutomo Nasu, Yoshiyuki Miyaji, Michihisa Saegusa, Katsuji Takeda, Hiromi Kumon

    WORLD JOURNAL OF UROLOGY   28 ( 4 )   413 - 418   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Although transurethral resection (TUR) is the standard treatment for non-muscle-invasive bladder tumors, 40-80% of tumors recur in spite of complete resection.
    To evaluate the efficacy, dose effectiveness and safety of early short-duration intravesical instillation therapy using epirubicin (EPI) administered immediately after TUR and on the next day following TUR.
    Between 1995 and 2001, 303 patients with Ta and T1 non-muscle-invasive bladder carcinoma were enrolled in this study. Patients were randomized into three groups. Group A patients were treated with two intravesical infusions of EPI 20 mg/40 ml saline immediately after TUR and within 24 h. Group B patients were treated with EPI 50 mg/100 ml on the same schedule as group A. Group C patients were treated by TUR alone as a control group. The primary endpoint was a duration to the first recurrence.
    Of the 303 patients, 79 in Group A, 84 in Group B, and 77 in Group C could be evaluated for recurrence. Median follow-up was 44 months. Median recurrence-free survival durations for Groups A, B, and C were 24, 38, and 13 months, respectively. The difference between Groups B and C was statistically significant (p = 0.04). Adverse reactions related to instillation were observed in about 30% of the patients. These reactions included micturition pain and frequency. These toxicities were mild and transient.
    Intravesical instillation of EPI 50 mg twice within 24 h after TUR was effective as prophylactic therapy for non-muscle-invasive bladder cancer with tolerable toxicity problems.

    DOI: 10.1007/s00345-009-0502-1

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  • 5αDH-DOC(5α-dihydro-deoxycorticosterone)activates androgen receptor in castration-resistant prostate cancer

    Uemura M, Honma S, Chung S, Takata R, Furihata M, Nishimura K, Nonomura N, Nasu Y, Miki T, Shuin T, Fujioka T, Okuyama A, Nakamura Y, Nakagawa H

    Cancer Sci   101 ( 8 )   1897 - 1904   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Potent antitumor effects of combined therapy with a telomerase-specific, replication-competent adenovirus (OBP-301) and IL-2 in a mouse model of renal cell carcinoma 査読

    P. Huang, H. Kaku, J. Chen, Y. Kashiwakura, T. Saika, Y. Nasu, Y. Urata, T. Fujiwara, M. Watanabe, H. Kumon

    CANCER GENE THERAPY   17 ( 7 )   484 - 491   2010年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    OBP-301 (a telomerase-specific, replication-competent adenovirus with hTERT promoter) was constructed in a previous study and it showed a strong anticancer effect by inducing cell lysis in human lung and prostate cancer cells. This study investigated the effectiveness of a combination therapy of OBP-301 and interleukin-2 (IL-2) in a mouse model of renal cell carcinoma (RCC). The cell-killing effect of OBP-301 was confirmed in vitro in the RENCA cancer cells. In in vivo experiment, luciferase-expressing RENCA cells were implanted in the left kidney and lung of BALB/c mice to prepare the RCC metastatic model. The animals were randomly divided into four treatment groups: PBS, IL-2 alone, OBP-301 alone and the combination. The analyses of orthotopic tumor weight, lung metastasis and luciferin-stained tumor images 14 days after each treatment showed significant tumor growth inhibition in the combination group in comparison with that in the OBP-301- or IL-2-treated groups. In addition, the percentage of regulatory T-cells (Tregs) in the combination group was significantly suppressed in comparison with that in the PBS and single-agent treatment groups. The outcomes of this study suggest that tumor-specific oncolytic immunovirotherapy may become an attractive strategy for the treatment of human RCC. Cancer Gene Therapy (2010) 17, 484-491; doi:10.1038/cgt.2010.5; published online 19 February 2010

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  • A randomized phase II traial of personalized peptide vaccine plus low dose estramustin phosphate(EMP)versus standard dose EMP in patients with castration resistant prostate cancer

    Noguchi M, Kakuma T, Uemura H, Nasu Y, Kumon H, Hirao Y, Moriya F, Suekane S, Matsuoka K, Komatsu N, Shichijo S, Yamada A, Itoh K

    Cancer Immunol Immunother   59 ( 7 )   1001 - 1009   2010年7月

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  • Down-regulation of BiP/GRP78 sensitizes resistant prostate cancer cells to gene-therapeutic overexpression of REIC/Dkk-3 査読

    Ryuta Tanimoto, Masakiyo Sakaguchi, Fernando Abarzua, Ken Kataoka, Kaoru Kurose, Hitoshi Murata, Yasutomo Nasu, Hiromi Kumon, Nam-Ho Huh

    INTERNATIONAL JOURNAL OF CANCER   126 ( 7 )   1562 - 1569   2010年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JOHN WILEY & SONS INC  

    We have recently shown that an adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The aim of the present study was to determine the molecular mechanisms of resistance to Ad-REIC. First, we isolated resistant clones from a human prostate cancer cell line, PC3, after repeated exposure to Ad-REIC. Infection efficiency of the adenovirus vector and expression level of REIC/Dkk-3 in the resistant clones were similar to those in the parental PC3 cells. By screening for alteration in levels and functional status of proteins involved in Ad-REIC-induced apoptosis, we found that BiP/GRP78, an ER-residing chaperone protein, was expressed at higher levels consistently among resistant cells. Expression levels of BiP and rates of apoptosis induced by Ad-REIC were inversely correlated. Down-regulation of BiP with siRNA sensitized the resistant cells to Ad-REIC in vivo as well as in culture. These results indicate that BiP is a major determinant of resistance to Ad-REIC-induced apoptosis. Thus BiP is useful for diagnosis of inherent and acquired resistance of cancers and also as a target molecule to overcome resistance to the gene therapeutic Ad-REIC.

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  • Frequent silencing of tumor suppressive MicroRNA-34b/c by aberrant methylation in malignant mesothelioma 査読

    Takafumi Kubo, Shinichi Toyooka, Kazunori Tsukuda, Hiroaki Asano, Masakiyo Sakaguchi, Junichi Soh, Tsuyoshi Ueno, Hiromasa Yamamoto, Masaomi Yamane, Takahiro Oto, Yasutomo Nasu, Hideki Matsui, Nam Ho Huh, Shinichro Miyoshi

    CANCER RESEARCH   70   2010年4月

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    記述言語:英語   出版者・発行元:AMER ASSOC CANCER RESEARCH  

    DOI: 10.1158/1538-7445.AM10-2081

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  • CLITORIAL BLOOD FLOW CHANGES AFTER SURGERY WITH TENSION-FREE VAGINAL MESH FOR PELVIC ORGAN PROLAPSE 査読

    Watanabe Toyohiko, Inoue Miyabi, Ishii Ayano, Yamato Toyoko, Nose Hiroyuki, Uematsu Katsutoshi, Sasaki Katsumi, Araki Motoo, Uehara Shinya, Saika Takashi, Nasu Yasutomo, Kumon Hiromi

    JOURNAL OF UROLOGY   183 ( 4 )   E583   2010年4月

  • Prognostic Factors Influencing Survival after Nephroureterectomy for Transitional Cell Carcinoma of the Upper Urinary Tract 査読

    Yasuyuki Kobayashi, Takashi Saika, Daisuke Manabe, Yasutomo Nasu, Hiromi Kumon

    ACTA MEDICA OKAYAMA   64 ( 1 )   27 - 31   2010年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We analyzed the prognostic factors influencing survival after surgeries for upper urinary tract urothelial carcinoma (UUT-UC) with longer follow-up periods than in previous studies. Between January 2000 and December 2004, 386 patients underwent nephroureterectomy for UUT-UC. The data for the 221 patients with UUT-UC were retrospectively reviewed. Nine variables were evaluated for association with the survival outcomes of cause-specific survival. The prognostic significance wits tested univariately with the log-rank test. The simultaneous effects of multiple prognostic factors were estimated by multiple regression analysis using the Cox proportional hazards model. The median follow-up was 38.4 months. The 5-year over all survival was 62.3%. Significant prognostic factors for disease-specific survival rate on univariate analysis were pathological stage (p < 0.0001), tumor grade (p = 0.0324), and venous invasion (p < 0.0001). Multivariate analysis revealed that only venous invasion was significant for disease-specific survival rate (p = 0.0205). Venous invasion wits the only independent prognostic factor in pathologrically localized UUT-UC.

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  • REIC/Dkk-3 stable transfection reduces the malignant phenotype of mouse prostate cancer RM9 cells

    Jie Chen, Masami Watanabe, Peng Huang, Masakiyo Sakaguchi, Kazuhiko Ochiai, Yasutomo Nasu, Mamoru Ouchida, Nam-Ho Huh, Kenji Shimizu, Yuji Kashiwakura, Haruki Kaku, Hiromi Kumon

    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE   24 ( 6 )   789 - 794   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    The reduced expression in immortalized cells (REIC)/Dickkopf (Dkk)-3. a member of the Dkk gene family, is a tumor suppressor in a broad range of cancers. REIC/Dkk-3 transfected stable clones of mouse prostate cancer RM9 cells (RM9-REIC) and the empty vector-transfected control clone cells (RM9-EV) were established. Clones were used to evaluate the anti-cancer effects and a proteomics analysis of REIC/Dkk-3 continuous expression was performed. The RM9-REIC cells show a feeble appearance and the cell membrane shows irregular buds known as blebs. In vitro cell proliferation was significantly suppressed in RM9-REIC clones in comparison to the control. The apoptosis assay was done under standard culture conditions and RM9-REIC showed a higher incidence of apoptosis. The RM9-EV and RM9-REIC cells were orthotopically implanted into a C57BL/6 mouse prostate. After 2 weeks, the tumor growth was significantly inhibited in RM9-REIC cells in comparison to the control. Two-dimensional gel electrophoresis was used to examine the modification of protein expression by the gene transfection. The analysis with mass spectrometry disclosed that expression of peroxiredoxin-l, GST-P1. transgelin-2, MRP-L12, ARD, GRP78 and Sorcin were increased and eEF1A-1 and cyclophilin-40 protein were decreased in RM9-REIC cells. Therefore, REIC/Dkk-3 stable transfectants show a reduction of malignancy in mouse prostate cancer RM9 cells in vitro and in vivo. The result of the proteomics analysis might provide important clues to clarify the anti-cancer molecular mechanism of REIC/Dkk-3 gene transfer.

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  • Dynasore, a dynamin inhibitor, suppresses lamellipodia formation and cancer cell invasion by destabilizing actin filaments 査読

    Hiroshi Yamada, Tadashi Abe, Shun-Ai Li, Yuki Masuoka, Mihoko Isoda, Masami Watanabe, Yasutomo Nasu, Hiromi Kumon, Akira Asai, Kohji Takei

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   390 ( 4 )   1142 - 1148   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Dynamic remodeling of actin filaments are bases for a variety of cellular events including cell motility and cancer invasion, and the regulation of actin dynamics implies dynamin, well characterized endocytotic protein. Here we report that dynasore, a inhibitor of dynamin GTPase, potently destabilizes F-actin in vitro, and it severely inhibits the formation of pseudopodia and cancer cell invasion, both of which are supported by active F-actin formation. Dynasore rapidly disrupted F-actin formed in brain cytosol in vitro, and the dynasore's effect on F-actin was indirect. Dynasore significantly suppressed serum-induced lamellipodia formation in U2OS cell. Dynasore also destabilized F-actin in resting cells, which caused the retraction of the plasma membrane. A certain amount of dynamin 2 in U2OS cells localized along F-actin, and co-localized with cortactin, a physiological binding partner of dynamin and F-actin. However. these associations of dynamin were partially disrupted by dynasore treatment. Furthermore, invasion activity of H1080 cell, a lung cancer cell line, was suppressed by approximately 40% with dynasore treatment. These results strongly suggest that dynasore potently destabilizes F-actin, and the effect implies dynamin. Dynasore or its derivative would be suitable candidates as potent anti-cancer drugs. (C) 2009 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2009.10.105

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  • A comparison of proteomic profiles changes during 17beta-estradiol treatment in human prostate cancer PC-3 cell line. 査読

    Chen J,Huang P, Kaku H, Zhang K, Watanabe M, Saika T, Nasu Y, Kumon H

    Cancer Genomics Proteomics   2009年11月

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  • Overexpression of the potential kinase serine/ threonine/tyrosine kinase 1 (STYK 1) in castration-resistant prostate cancer

    Chung S, Tamura K, Furihata M, Uemura M, Daigo Y, Nasu Y, Miki T, Shuin T, Fujioka T, Nakamura Y, Nakagawa H

    Cancer Sci   100 ( 11 )   2109 - 2114   2009年11月

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  • The role of hepatocyte growth factor activator inhibitor-1(HAI-1)as a prognostic indicator in cervical cancer

    Nakamura K, Abarzua F, Hongo A, Kodama J, Nasu Y, Kumon H, Hiramatsu Y

    Int J Oncol   35 ( 2 )   239 - 248   2009年8月

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  • Overexpressing PKIB in prostate cancer promotes its aggressiveness by linking between PKA and Akt pathways

    Chung S, Furihata M, Tamura K, Uemura M, Daigo Y, Nasu Y, Miki T, Shuin T, Fujioka T, Nakamura Y, Nakagawa H

    Oncogene   28 ( 32 )   2849 - 2859   2009年8月

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  • Prospective Longitudinal Comparative Study of Health-Related Quality of Life in Patients Treated with Radical Prostatectomy or Permanent Brachytherapy for Prostate Cancer 査読

    Makoto Kobuke, Takashi Saika, Yoshiko Nakanishi, Shin Ebara, Daisuke Manabe, Tatsuya Uesugi, Hiroyuki Nose, Ryoji Arata, Tomoyasu Tsushima, Yasutomo Nasu, Hiromi Kumon

    ACTA MEDICA OKAYAMA   63 ( 3 )   129 - 135   2009年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    To determine health-related quality of life (HRQOL) after radical retropubic prostatectomy (RRP) or permanent prostate brachytherapy (BT), third party-conducted QOL surveys were prospectively compared. Between 2004 and 2005, 37 patients underwent RRP and 36 were treated with BT. A QOL survey consisting of the Medical Outcomes Study 36-Item Short Form (SF-36), the University of California, Los Angeles, Prostate Cancer Index (UCLA-PCI) and the International Prostate Symptoms Score (IPSS) was completed prospectively by a research coordinator at baseline, and at 1, 3, 6 and 12 months after treatment. The RRP patients scored well in general QOL except at I month after surgery, with their mental health better than at baseline by 6 months after surgery. Disease-specific QOL in RRP patients received a low score at I month for both urinary and sexual function, though urinary function rapidly recovered to baseline levels. BT patient QOL was not affected by the therapy except in the IPSS score. However, general and mental health scores in BT patients were inferior to those in RRP patients. This prospective study revealed differences in QOL after RRP and BT. These results will be helpful in making treatment decisions.

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  • Overexpression of REIC/Dkk-3 in Normal Fibroblasts Suppresses Tumor Growth via Induction of Interleukin-7

    Masakiyo Sakaguchi, Ken Kataoka, Fernando Abarzua, Ryuta Tanimoto, Masami Watanabe, Hitoshi Murata, Swe Swe Than, Kaoru Kurose, Yuji Kashiwakura, Kazuhiko Ochiai, Yasutomo Nasu, Hiromi Kumon, Nam-ho Huh

    JOURNAL OF BIOLOGICAL CHEMISTRY   284 ( 21 )   14236 - 14244   2009年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    We previously showed that the tumor suppressor gene REIC/Dkk-3, when overexpressed by an adenovirus (Ad-REIC), exhibited a dramatic therapeutic effect on human cancers through a mechanism triggered by endoplasmic reticulum stress. Adenovirus vectors show no target cell specificity and thus may elicit unfavorable side effects through infection of normal cells even upon intra-tumoral injection. In this study, we examined possible effects of Ad-REIC on normal cells. We found that infection of normal human fibroblasts (NHF) did not cause apoptosis but induced production of interleukin (IL)-7. The induction was triggered by endoplasmic reticulum stress and mediated through IRE1 alpha, ASK1, p38, and IRF-1. When Ad-REIC-infected NHF were transplanted in a mixture with untreated human prostate cancer cells, the growth of the cancer cells was significantly suppressed. Injection of an IL-7 antibody partially abrogated the suppressive effect of Ad-REIC-infected NHF. These results indicate that Ad-REIC has another arm against human cancer, an indirect host-mediated effect because of overproduction of IL-7 by mis-targeted NHF, in addition to its direct effect on cancer cells.

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  • Expression of Matriptase and Clinical Outcome of Human Endometrial Cancer 査読

    Keiichiro Nakamura, Atsushi Hongo, Junichi Kodama, Fernando Abarzua, Yasutomo Nasu, Hiromi Kumon, Yuji Hiramatsu

    ANTICANCER RESEARCH   29 ( 5 )   1685 - 1690   2009年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT INST ANTICANCER RESEARCH  

    Background: Matriptase, a type II transmembrane serine protease is involved in angiogenesis, degradation of extracellular matrix and in progression of some epithelial cancers. The purpose of the present study was to examine matriptase expression and evaluate its clinicopathological significance in endometrial cancer. Patients and Methods: Matriptase expression was examined in normal endometrium (n=20), endometrial hyperplasia (n=11) and endometrial cancer (n=65) samples. The distribution of cases that scored positive for each of the biological parameters examined was correlated with matriptase expression status obtained by immunohistochemistry. Results: Matriptase was found to be significantly overexpressed in endometrial cancer as compared with normal endometrium and endometrial hyperplasia. Interestingly, matriptase expression is associated with stage (p=0.010), grade (p=0.021), depth of myometrial invasion (p=0.004), cervical involvement (p=0.021), lymph node metastasis (p=0.009), LVS involvement (p=0.041) and peritoneal cytology (p=0.045). The high matriptase expression was a significant predictor for poor prognosis when compared with low matriptase expression (Disease-free survival rate; p=0.032, Overall survival rate; p=0.011). Conclusion: High matriptase expression in endometrial cancer may be associated with poor prognosis.

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  • Stanniocalcin 2 overexpression in castration-resistant prostate cancer and aggressive prostate cancer

    Tamura K, Furihata M, Chung S.Y, Uemura M, Yoshioka H, Iiyama T, Ashida S, Nasu Y, Fujioka T, Shuin T, Nakamura Y, Nakagawa H

    Cancer Sci   100 ( 5 )   914 - 919   2009年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Prostate-specific antigen 'bounce' after permanent 125I -implant brachytherapy in Japanese men:a multi-institutional pooled analysis

    Satoh T, Ishiyama H, Matsumoto K, Tsumura H, Kitano M, Hayakawa K, Ebara S, Nasu Y, Kumon H, Kanazawa S, Miki K, Egawa S, Aoki M, Toya K, Yorozu A, Nagata H, Saito S, Baba S

    BJU International   103 ( 8 )   1064 - 1068   2009年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Immunological aspects of REIC/Dkk-3 in monocyte differentiation and tumor regression

    Masami Watanabe, Yuji Kashiwakura, Peng Huang, Kazuhiko Ochiai, Junichiro Futami, Shun-Ai Li, Munenori Takaoka, Yasutomo Nasu, Masakiyo Sakaguchi, Nam-Ho Huh, Hiromi Kumon

    INTERNATIONAL JOURNAL OF ONCOLOGY   34 ( 3 )   657 - 663   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    The REIC/Dkk-3 gene has been reported to be a tumor suppressor and the expression is significantly down-regulated in a broad range of cancer cell types. The protein is secretory, but the physiological function remains unclear. This study demonstrated that recombinant REIC/Dkk-3 protein induced the differentiation of human CD14(+) monocytes into a novel cell type ((REIC/Dkx-3)Mo). (REIC/Dkk-3)Mo resembles immature dendritic cells generated with IL-4 and GM-CSF. Both these cell populations exhibit similar proportions of CD11c(+), CD40(+), CD86(+) and HLA-DR(+) cells and endocytic capacity, but (REIC/Dkk-3)Mo is negative for CD1a antigen. An analysis of the signal transducers and activators of transcription (STAT) pathways revealed that REIC/Dkk-3 induces phosphorylation of STAT 1 and STAT 3. Furthermore, intratumoral administration of REIC/Dkk-3 protein significantly suppressed tumor growth with CD11c(+) and CD8(+) (dendritic and killer T cell marker, respectively) cell accumulation and enhanced anticancer cytolytic activity of splenocytes. These data indicated a cytokine-like role of REIC/Dkk-3 protein in monocyte differentiation that might be exploited therapeutically.

    DOI: 10.3892/ijo_00000191

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  • Expression of hepatocyte growth factor activator inhibitors (HAI-1 and HAI-2) in ovarian cancer

    Nakamura K, Abarzua F, Kodama J, Hongo A, Nasu Y, Kumon H, Hiramatsu Y

    Int J Oncol   34 ( 2 )   345 - 353   2009年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Hepatocyte growth factor activator inhibitor-2(HAI-2)is a favorable prognosis marker and inhibits cell growth through the apoptotic pathway in cervical cancer

    Nakamura K, Abarzua F, Hongo A, Kodama J, Nasu Y, Kumon H, Hiramatsu Y

    Annals of Oncology   20 ( 1 )   63 - 70   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Maximum androgen blockade中のPSA再燃に対する非ステロイド性抗アンドロゲン剤交替療法 NASA-PC研究会報告を中心に

    藤本 直浩, 松本 哲朗, 鈴木 啓悦, 市川 智彦, 沖原 宏治, 三木 恒治, 三宅 秀明, 藤澤 正人, 植村 元秀, 三好 進, 田丁 貴俊, 矢野 明, 藤井 元廣, 滝花 義男, 武田 正之, 石 光広, 松原 昭郎, 碓井 亞, 木下 秀文, 松田 公志, 麦谷 荘一, 大園 誠一郎, 那須 保友, 雑賀 隆史, 公文 裕巳

    西日本泌尿器科   71 ( 1 )   3 - 10   2009年1月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • Initial report of hybrid radical prostatectomy for prostate cancer: reduced bleeding, clear vision, and secure surgical margins. 査読

    Saika T, Kobayashi Y, Watanabe T, Manabe D, Ebara S, Uehara S, Nasu Y, Kumon H

    Acta medica Okayama   62 ( 6 )   379 - 384   2008年12月

  • Mechanistic analysis of resistance to REIC/Dkk-3-induced apoptosis in human bladder cancer cells. 査読

    Kobayashi T, Sakaguchi M, Tanimoto R, Abarzua F, Takaishi M, Kaku H, Kataoka K, Saika T, Nasu Y, Miyazaki M, Kumon H, Huh NH

    Acta medica Okayama   62 ( 6 )   393 - 401   2008年12月

  • An N-terminal 78 amino acid truncation of REIC/Dkk-3 effectively induces apoptosis

    Fernando Abarzua, Yuji Kashiwakura, Munenori Takaoka, Masami Watanabe, Kazuhiko Ochiai, Masakiyo Sakaguchi, Takao Iwawaki, Ryuta Tanimoto, Yasutomo Nasu, Nam-ho Huh, Hiromi Kumon

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   375 ( 4 )   614 - 618   2008年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Overexpression of REIC/Dkk-3 (a tumor suppressor gene) induces cancer cell apoptosis through endoplasmic reticulum (ER) stress. Therefore, the identification of the portion of REIC/Dkk-3 that causes ER stress may be essential for the development of cancer treatment based on REIC/Dkk-3. Here, we made several truncated forms of REIC/Dkk-3 and investigated their therapeutic potentials against prostate cancer. Among three truncated forms, a variant comprising the N-terminal 78 amino acid region of REIC/Dkk-3 ((1-78) REIC/Dkk-3) most strongly induced ER stress and apoptosis in human prostate cancer cells (PC3). For in vivo gene expression, we coupled a biodegradable polymer with naked DNA, which attained robust trans-gene expression in PC3-derived subcutaneous tumor. In therapeutic experiments, we demonstrated that multiple direct injections of polymer-conjugated (1-78)REIC/Dkk-3 plasmid provoke ER stress and significantly reduced the subcutaneous tumor volume compared with the control group. We suggest this non-viral strategy may be an effective alternative to viral gene therapy. (C) 2008 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2008.08.079

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  • Down-regulation of Inhibition of Differentiation-1 via Activation of Activating Transcription Factor 3 and Smad Regulates REIC/Dickkopf-3-Induced Apoptosis

    Yuji Kashiwakura, Kazuhiko Ochiai, Masami Watanabe, Fernando Abarzua, Masakiyo Sakaguchi, Munenori Takaoka, Ryuta Tanimoto, Yasutomo Nasu, Nam-ho Hub, Hiromi Kumon

    CANCER RESEARCH   68 ( 20 )   8333 - 8341   2008年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC CANCER RESEARCH  

    REIC/Dickkopf-3 (Dkk-3), a tumor suppressor gene, has been investigated in gene therapy studies. Our previous study suggested that REIC/Dkk-3-induced apoptosis mainly resulted from phosphorylation of c-Jun-NH2 kinase (JNK) in prostate cancer cells. However, the precise mechanisms, especially the molecular mechanisms regulating JNK phosphorylation, remain unclear. In this study, we investigated the mechanisms participating in JNK phosphorylation in the context of a refractory cancer disease, malignant mesothelioma (MM). Adenovirus-mediated overexpression of REIC/Dkk-3 induced apoptosis mainly through JNK activation in immortalized MM cells (211H cells). Interestingly, transcriptional down-regulation of inhibition of differentiation-1 (Id-1) was detected in REIC/Dkk-3-overexpressed 211H cells. Moreover, restoration of Id-1 expression antagonized REIC/Dkk-3-induced JNK phosphorylation and apoptosis. Mutagenesis experiments with the 2.1-kb human Id-1 promoter revealed that activating transcription factor 3 (ATF3) and Smad interaction, with their respective binding motifs, was essential for REIC/Dkk-3-mediated suppression of Id-1 promoter activity. ATF3 activation was probably induced by endoplasmic reticulum stress. Finally, we showed strong antitumor effects from REIC/Dkk-3 gene transfer into the pleural cavity in an orthotopic MM mouse model. Relative to control tumor tissue, REIC/Dkk-3-treated tumor tissue showed down-regulated expression of Id-1 mRNA, enhanced expression of phosphorylated JNK, and an increased number of apoptotic cells. In summary, we first showed that both ATF3 and Smad were crucially and synergistically involved in down-regulation of Id-1, which regulated JNK phosphorylation in REIC/Dkk-3-induced apoptosis. Thus, gene therapy with REIC/Dkk-3 may be a promising therapeutic tool for MM. [Cancer Res 2008;68(20):8333-41]

    DOI: 10.1158/0008-5472.CAN-08-0080

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  • The benefits of clamping the renal artery in laparoscopic partial nephrectomy 査読

    Yasuyuki Kobayashi, Takashi Saika, Daisuke Manabe, Yasutomo Nasu, Hiromi Kumon

    ACTA MEDICA OKAYAMA   62 ( 4 )   269 - 273   2008年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    The purpose of this study is to compare the performance of laparoscopic partial nephrectomy (LPN) with and without clamping of the renal artery and to evaluate the impact of clamping on postoperative renal function. A total of 20 patients underwent LPN, 13 without and 7 with clamping of the renal artery. The 2 groups were compared with respect to complications, blood loss, operative time, mean tumor size, and incidence of positive margins. Renal function was evaluated by pre- and postoperative renal scintigraphy using (99m)Technetium-mercaptoacetyltriglycine (Tc-99m-MAG3). Intraoperative blood loss was significantly higher in the group without clamping than in the group with clamping (p = 0.04). In the group with clamping, the median warm ischemic time was 35 min (range 25-40 min). The serum creatinine values and the renal scintigraphy showed no influence on postoperative renal function with or without clamping. In the group without clamping, 2 cases were showed positive surgical margins. The procedure performed with clamping of the renal artery is superior to the procedure performed without clamping as it provides the advantages of controlling hemorrhaging without injury to renal function and prolonging the surgical time and allowing for more accurate resection of renal tumors.

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  • Adenovirus-mediated REIC/DKK-3 gene transfer prevented mesothelioma tumor progressions in orthotopic mice model 査読

    Kashiwakura Yuji, Watanabe Masami, Abarzua Fernando, Sakaguchi Masakiyo, Takaoka Munenori, Tanimoto Ryuta, Nasu Yasutomo, Huh Nam-ho, Kumon Hiromi

    JOURNAL OF GENE MEDICINE   10 ( 4 )   469 - 470   2008年4月

  • REIC/DKK-3 as a potential gene therapeutic agent against human testicular cancer 査読

    Tanimoto Ryuta, Abarzua Fernando, Sakaguchi Masakiyo, Nasu Yasutomo, Kumon Hiromi, Huh Nam-Ho

    JOURNAL OF GENE MEDICINE   10 ( 4 )   454   2008年4月

  • A comparative study of the oncological long-term outcome of laparoscopic nephroureterectomy and standard nephroureterectomy for upper urinary tract transitional cell carcinoma 査読

    Kobayashi Yasuyuki, Manabe Daisuke, Uehara Shinya, Watanabe Toyohiko, Saika Takashi, Nasu Yasutomo, Kumon Hiromi

    JOURNAL OF UROLOGY   179 ( 4 )   291   2008年4月

  • A rare tumor in the adrenal region: Neuron-specific enolase (NSE)-producing leiomyosarcoma in an elderly hypertensive patient 査読

    Junko Goto, Fumio Otsuka, Ryo Kodera, Tomoko Miyoshi, Masaru Kinomura, Hiroyuki Otani, Yukari Mimura, Toshio Ogura, Hiroyuki Yanai, Yasutomo Nasu, Hirofumi Makino

    ENDOCRINE JOURNAL   55 ( 1 )   175 - 181   2008年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN ENDOCRINE SOC  

    A 73-year-old Japanese woman was referred for examination of right flank pain and progressive hypertension. Abdominal CT incidentally detected a right adrenal mass 8 cm in size. The tumor exhibited isodensity by CT and contained high-intense lesion by T2-weighted MRI. Scintigraphy with I-131-metaiodobenzylguanidine and I-131-adosterol showed no abnormal uptake by whole body scan. Positron emission tomography scan with F-18-2-fluoro-D-deoxyglucose demonstrated an exclusive uptake in the right adrenal mass. Adrenocortical hormone levels and catecholamine secretion were within normal range; however, the level of serum neuron-specific enolase (NSE) was found to be markedly high. After controlling systemic blood pressure with an alpha 1-blocker, the right adrenal tumor was surgically removed, along with the right kidney and inferior vena cava which adhered to it. The tumor was pathologically proven to be leiomyosarcoma, which was immunohistochemically positive with a-smooth muscle actin and negative with CD57, S-100 and c-kit proteins. Notably, NSE protein was massively expressed in the resected tumor. After surgery blood pressure was controlled with regular medication and serum NSE levels have since normalized. The possibility of leiomyosarcoma should be kept in mind in adrenal incidentalomas with rapid growth and atypical radiological images. Our findings suggest that circulating NSE levels may be clinically useful for early detection of recurrence.

    DOI: 10.1507/endocrj.K07E-020

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  • Iodine-125 seed implantation (permanent brachytherapy) for clinically localized prostate cancer 査読

    Shin Ebara, Yoshihisa Katayama, Ryuta Tanimoto, Kohei Edamura, Hiroyuki Nose, Daisuke Manabe, Tomoko Kobayashi, Yasuyuki Kobayashi, Makoto Kobuke, Mitsuhiro Takemoto, Takashi Saika, Yasutomo Nasu, Susumu Kanazawa, Hiromi Kumon

    ACTA MEDICA OKAYAMA   62 ( 1 )   9 - 13   2008年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    From January 2004 to March 2007, 308 patients with clinically localized prostate cancer were treated using iodine-125 (I-125) seed implantation (permanent brachytherapy) at Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. We evaluated the treatiment's efficacy and morbidity in 300 prostate cancer patients who were followed up for more than I month after brachytherapy. Based on the National Comprehensive Cancer Network (NCCN) guidelines, patients with a prostate volume of less than 40 ml in transrectal ultrasound imaging were classified as low or intermediate risk. The median patient age was 67 years (range 50 to 79 years), the median prostate-specific antigen (PSA) value before biopsy was 6.95 ng/ml (range 1.13 to 24.7 ng/ml), and the median prostate volume was 24.33 ml (range 9.3 to 41.76 ml). The median follow-up was 18 months (range 1 to 36 months) and the PSA levels decreased in almost all patients after brachytherapy. Although 194 of 300 patients (64.7%) complained of difficulty in urination, pollakisuria/urgency, miction pain, and/or urinary incontinence, all of which might be associated with radiation prostatitis during the first month after brachytherapy, these symptoms gradually improved. I-125 seed implantation brachytherapy is safe and effective for localized prostate cancer within short-term follow up.

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  • Expression of the serine protease hepsin and clinical outcome of human endometrial cancer

    Matsuo T, Nakamura K, Takamoto N, Kodama J, Hongo A, Abrzua F, Nasu Y, Kumon H, Hiramatsu Y

    Anticancer Research   28   159 - 164   2008年

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  • New highly potent and specific E6 and E7 siRNAs for treatment of HPV16 positive cervical cancer

    Yamato K, Yamada T, Kizaki M, Ui-Tei K, Natori Y, Fujino M, Nishihara T, Ikeda Y, Nasu Y, Saigo K, Yoshinouchi M

    Cancer Gene Therapy   15   140 - 153   2008年

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  • Secretory leukoprotease inhibitor inhibits cell growth through apoptotic pathway on ovarian cancer

    Nakamura K, Takamoto N, Hongo A, Kodama J, Abrzua F, Nasu Y, Kumon H, Hiramatsu Y

    Oncol Rep   19 ( 5 )   1085 - 1091   2008年

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  • Hepsin inhibits the cell growth of endometrial cancer

    Nakamura K, Takamoto N, Abarzua F, Hongo A, Kodama J, Nasu Y, Kumon H, Hiramatsu Y

    Int J Mol Med   22 ( 3 )   389 - 397   2008年

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  • REIC/Dkk-3 overexpression downregulates P-glycoprotein in multidrug-resistant MCF7/ADR cells and induces apoptosis in breast cancer.

    Kawasaki K, Watanabe M, Sakaguchi M, Ogasawara Y, Ochiai K, Nasu Y, Doihara H, Kashiwakura Y, Huh NH, Kumon H, Date H

    Cancer Gene Ther.   16 ( 1 )   65 - 72   2008年

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    The overexpression of reduced expression in immortalized cells (REIC)/Dickkopf-3 (Dkk-3), a tumor suppressor gene, induced apoptosis in human prostatic and testicular cancer cells. The aim of this study is to examine the potential of REIC/Dkk-3 as a therapeutic target against breast cancer. First, the in vitro apoptotic effect of Ad-REIC treatment was investigated in breast cancer cell lines and the adenovirus-mediated overexpression of REIC/Dkk-3 was thus found to lead to apoptotic cell death in a c-Jun-NH2-kinase (JNK) phosphorylaion-dependent manner. Moreover, an in vivo apoptotic effect and MCF/Wt tumor growth inhibition were observed in the mouse model after intratumoral Ad-REIC injection. As multidrug resistance (MDR) is a major problem in the chemotherapy of progressive breast cancer, the in vitro effects of Ad-REIC treatment were investigated in terms of the sensitivity of multidrug-resistant MCF7/ADR cells to doxorubicin and of the P-glycoprotein expression. Ad-REIC treatment in MCF7/ADR cells also downregulated P-glycoprotein expresssion through JNK activation, and sensitized its drug resistance against doxorubicin. Therefore, not only apoptosis induction but also the reversal of anticancer drug resistance was achieved using Ad-REIC. We suggest that REIC/Dkk-3 is a novel target for breast cancer treatment and that Ad-REIC might be an attractive agent against drug-resistant cancer in combination with conventional antineoplastic agents. © 2009 Nature Publishing Group All rights reserved.

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  • Routine transurethral biopsy of the bladder is not necessary to evaluate the response to bacillus Calmette-Guerin therapy 査読

    Takanori Murakami, Shin Ebara, Takashi Saika, Shin Irie, Katsuji Takeda, Yoshio Maki, Sadayuki Miyaji, Daisuke Manabe, Haruki Kaku, Yasutomo Nasu, Tomoyasu Tsushima, Hiromi Kumon

    ACTA MEDICA OKAYAMA   61 ( 6 )   341 - 344   2007年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We evaluated the need for transurethral biopsy at first follow-up after intravesical. bacillus Calmette-Guerin (BCG) therapy for superficial bladder cancer. The records of 84 patients with superficial bladder cancer who received a 6- or 8-week course of BCG were reviewed. Pathological results before BCG, cystoscopic findings, urinary cytology, and biopsy results for evaluation of BCG therapy were reviewed. All 19 patients with positive urinary cytology had evidence of positive bladder biopsy results. Fifty-three of 54 patients (98.1 %) with no visible recurrent tumor and negative urinary cytology demonstrated negative pathological results on bladder biopsy. When not found in conjunction with positive urinary cytology, erythematous mucosa on cystoscopy was not an indicator of tumor recurrence or residual cancer. In conclusion, routine transurethral biopsy of the bladder for evaluating the response to BCG intravesical therapy is not necessary in patients who have no visible tumor on cystoscopy and negative urinary cytology.

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  • The efficacy of neoadjuvant androgen deprivation therapy as a prostate volume reduction before brachytherapy for clinically localized prostate cancer 査読

    Shin Ebara, Daisuke Manabe, Yasuyuki Kobayashi, Ryuta Tanimoto, Takashi Saika, Yasutomo Nasu, Shirou Saito, Takefumi Satoh, Kenta Miki, Katsuyoshi Hashine, Hiromi Kumon

    ACTA MEDICA OKAYAMA   61 ( 6 )   335 - 340   2007年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    From September 2003 to December 2005, 188 patients who visited our hospital and allied institutions for the purpose of prostate brachytherapy were administrated hormonal therapy for volume reductions before brachytherapy. The pretreatment and posttreatment of prostate volume using a transrectal ultrasound volumetric study and the types and duration of hormonal therapy were analyzed. We administered 91 patients with Luteinizing hormone-releasing hormone (LH-RH) agonis, 49 patients with anti-androgen (bicaltamide/flutamide), and 48 patients with maximum androgen blockade (MAB). The duration of the hormonal therapy was 1-3 months for 49 patients, 4-6 months for 59 patients, 7-9 months for 40 patients, 10-12 months for 32 patients, and over 13 months for 8 patients. Before the initiation of hormonal therapy, the mean prostate volume was 35.12 ml (11.04-78.71 ml), and the average of prostate volume before and after hormonal therapy was 36.79 ml and 24.79 ml, respectively (a 32.4 % reduction). The prostate volume reduction rate was 32.0 % for the LH-RH agonist only, 18.1 % for the anti-androgen only and 41.2 % for the NUB. No statistically significant difference was observed for the duration of hormonal therapy between 3 groups. A three-month course of the neoadjuvant LH-RH agonist indicated a sufficient volume reduction effectiveness for a large prostate volume.

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  • Adenovirus-mediated REIC/Dkk-3 gene transfer inhibits tumor growth and metastasis in an orthotopic prostate cancer model 査読

    K. Edamura, Y. Nasu, M. Takaishi, T. Kobayashi, F. Abarzua, M. Sakaguchi, Y. Kashiwakura, S. Ebara, T. Saika, M. Watanabe, N-H Huh, H. Kumon

    CANCER GENE THERAPY   14 ( 9 )   765 - 772   2007年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    We had previously reported that REIC/Dkk-3, a member of the Dickkopf ( Dkk) gene family, works as a tumor suppressor. In this study, we evaluated the therapeutic effects of an intratumoral injection with adenoviral vector encoding REIC/Dkk-3 gene ( Ad-REIC) using an orthotopic mouse prostate cancer model of RM-9 cells. We also investigated the in vivo anti-metastatic effect and in vitro anti-invasion effect of Ad-REIC gene delivery. We demonstrated that the Ad-REIC treatment inhibited prostate cancer growth and lymph node metastasis, and prolonged mice survival in the model. These therapeutic responses were consistent with the intratumoral apoptosis induction and in vitro suppression of cell invasion/migration with reduced matrix metalloprotease-2 activity. We thus concluded that in situ Ad-REIC/Dkk-3 gene transfer may be a promising therapeutic intervention modality for the treatment of prostate cancer.

    DOI: 10.1038/sj.cgt.7701071

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  • Health-related quality of life after radical cystectomy for bladder cancer in elderly patients with an ileal conduit, ureterocutaneostomy, or orthotopic urinary reservoir: A comparative questionnaire survey 査読

    Takashi Saika, Ryoji Arata, Tomoyasu Tsushima, Yasutomo Nasu, Bunzo Suyama, Katsuji Takeda, Shin Ebara, Daisuke Manabe, Tomoko Kobayashi, Ryuta Tanimoto, Hiromi Kumon

    ACTA MEDICA OKAYAMA   61 ( 4 )   199 - 203   2007年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    To compare the health-related quality of life of elderly patients after radical cystectomy for bladder cancer in urinary diversion groups: ileal conduit, ureterocutaneostomy, or orthotopic urinary reservoir. The 109 participating elderly patients aged 75 or older completed self-reporting questionnaires: the QNQ-C30, and on satisfaction with urinary diversion methods. Fifty-six patients had undergone constructions for ileal conduit diversion, 31 for ureterocutaneostomy, and 22 for orthotopic urinary reservoir (OUR). The median follow-up period for each group was 4.0 years (range 0.3-11.2), 4.5 years (range 0.3-18.0), and 3.3 years (range 0.3-6.7), respectively. Regardless of the type of urinary diversion, the majority of patients reported having good overall quality of life, although with some problem of pain. No significant differences among urinary diversion subgroups were found in any quality of life area in the QLQ-C30 questionnaire. More patients in the OUR sub-group felt disappointment than those in the ileal conduit or cutaneostomy sub-groups. However, a questionnaire which asked which diversion method would be preferable showed a trend that more patients in the OUR subgroup would have chosen the same one. Health-related quality of life appeared relatively good in these 3 groups. Patient demands and expectations may be so different from the results that the details of each urinary diversion method should be explained thoroughly. OUR construction could be a candidate even for elderly patients.

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  • Orthotopic ileal neobladder versus sigmoidal neobladder: A "Quality of life" (QOL) survey 査読

    Ryoji Arata, Takashi Saika, Tomoyasu Tsushima, Fernando Abarzua, Yasutomo Nasu, Hiromi Kumon

    ACTA MEDICA OKAYAMA   61 ( 4 )   229 - 234   2007年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    To compare the quality of life (QOL) in patients with ileal neobladder and sigmoidal neobladder, a retrospective survey was conducted using a formulated questionnaire. Between January and March 1999, a QOL survey was conducted using self-administered questionnaires (EORTC QLQ-C30, IPSS, supplemented with detailed questionnaires about continence, sexual function, and patient's satisfaction with the selected urinary diversion method) for 78 patients with orthotopic urinary reservoir (OUR) who were followed-up for more than 3 months after cystectomy. Among 78 patients, 63 had OUR using an ileal segment (male/female 59/4, median age: 70.8 years old, median follow-up: 1.7 years). Fifteen patients had OUR using a sigmoidal segment (male/female = 13/2, median age: 71.9, median follow-up: 3.9). The QLQ-C30 functional evaluation and the items in relation to sexual function showed no differences between the 2 groups. Concerning the voiding condition, bladder emptying, frequency, and urgency, scores in the sigmoidal OUR group were significantly higher. The QOL score concerning voiding conditions, daytime, and nighttime continence and quantity of pad showed a better score in the ileal OUR group. Concerning the satisfaction with methods of urinary diversion, patients in the sigmoidal OUR group expressed less satisfaction than their preoperative expectations. Considering several postoperative voiding conditions, ileal OUR seems superior to sigmoidal OUR.

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  • Natural course of lower urinary tract symptoms following discontinuation of alpha-1-adrenergic blockers in patients with benign prostatic hyperplasia 査読

    Teruhiko Yokoyama, Toyohiko Watanabe, Takashi Saika, Yasutomo Nasu, Hiromi Kumon, Yoshiyuki Miyaji, Atsushi Nagai

    INTERNATIONAL JOURNAL OF UROLOGY   14 ( 7 )   598 - 601   2007年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    Objectives: alpha 1-adrenergic blockers (alpha b) remain the first-line therapy in men with lower urinary tract symptoms (LUTS). The current published work advocates continued use of alpha b for their effect to be maintained. However, some patients decide to discontinue use of the medication after their symptoms are relieved and can keep good conditions. In this study, we investigated the natural course of LUTS after the discontinuation of successful treatment of alpha b.
    Methods: Among 75 patients with LUTS who stopped alpha b medication once their symptoms improved, 60 patients (age, 50-87 years; median, 70) who could be followed for at least 12 months after discontinuation of alpha b were analyzed in this study. Evaluations included a clinical determination of the International Prostate Symptom Score (IPSS), peak flow rate (Qmax) and postvoid residual urine volume (PVR). Upon patient request or in cases of PVR more than 100 mL, administration of alpha b was resumed.
    Results: Eighteen out of the 60 patients (30%) asked for re-treatment within 12 months after discontinuation (re-treatment group). The other 42 patents were able to maintain good condition without medication (discontinuation group). The IPSS was 15.9, 8.7, 10.1, 10.2, 9.7, 8.8 and 9.0, on the first visit, just before discontinuation, and 1, 3, 6, 9 and 12 months after stopping treatment among the discontinuation group, respectively. Similarly, Qmax was 10.6, 14.8, 14.2, 14.3, 14.7, 13.2 and 13.6 mL/ s, respectively. Treatment periods, prostatic volume and peak flow rates just before discontinuation of medication differed significantly between the re-treatment and discontinuation group.
    Conclusions: In spite of the short follow-up periods, these results suggest that selected patients with relatively small prostatic volume and good flow rates after therapy can discontinue alpha b medication after their symptoms improve.

    DOI: 10.1111/j.1442-2042.2007.01784.x

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  • Heat shock proteins play a crucial role in tumor-specific apoptosis by REIC/Dkk-3 査読

    Fernando Abarzua, Masakiyo Sakaguchi, Ryuta Tanimoto, Hiroyuki Sonegawa, Dai-Wei L, Kohei Edamura, Tomoko Kobayashi, Masami Watanabe, Yuji Kashiwakura, Haruki Kaku, Takashi Saika, Keiichiro Nakamura, Yasutomo Nasu, Hiromi Kumon, Nam-Ho Huh

    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE   20 ( 1 )   37 - 43   2007年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PROFESSOR D A SPANDIDOS  

    We recently showed that overexpression of REIC/Dickkopf-3 (Dkk-3), a tumor suppressor gene, induced apoptosis in a tumor cell-specific manner. The aim of the present study was to determine the mechanisms underlying the selective induction of apoptosis. At first, we found a mouse renal carcinoma cell line, RENCA, to be extremely sensitive to an adenovirus carrying REIC/Dkk-3 (Ad-REIC), and we showed that activation of c-Jun N-terminal kinase (JNK) was a critical step in cell death, i.e. a process similar to that in human prostate and testicular cancer observed in our previous studies. Among the proteins interfering with the activation of JNK, heat shock protein (Hsp)70/72 was reduced in expression in RENCA cells compared with that in NIH3T3 cells. An Hsp70/72 inducer protected RENCA cells from Ad-REIC-induced apoptosis, while an Hsp70/72 inhibitor sensitized NIH3T3 cells for apoptosis induction. These results indicate that functionally active Hsp70/72 is a key factor in tumor cell-specific induction of apoptotic cell death and that analyses of the expression levels of Hsp70/72 may be essential in determining the significance of Ad-REIC-based gene therapy against human cancer.

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  • Molecular features of hormone-refractory prostate cancer cells by genome-wide gene expression profiles 査読

    Kenji Tamura, Mutsuo Furihata, Tatsuhiko Tsunoda, Shingo Ashida, Ryo Takata, Wataru Mara, Hiroki Yoshioka, Yataro Daigo, Yasutomo Nasu, Hiromi Kumon, Hiroyuki Konaka, Mikio Namiki, Keiichi Tozawa, Kenjiro Kohri, Nozomu Tanji, Masayoshi Yokoyama, Toru Shimazui, Hideyuki Akaza, Yoichi Mizutani, Tsuneharu Miki, Tomoaki Fujioka, Taro Shuin, Yusuke Nakamura, Hidewaki Nakagawa

    CANCER RESEARCH   67 ( 11 )   5117 - 5125   2007年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC CANCER RESEARCH  

    One of the most critical issues in prostate cancer clinic is emerging hormone-refractory prostate cancers (HRPCs) and their management. Prostate cancer is usually androgen dependent and responds well to androgen ablation therapy. However, at a certain stage, they eventually acquire androgen-independent and more aggressive phenotype and show poor response to any anticancer therapies. To characterize the molecular features of clinical HRPCs, we analyzed gene expression profiles of 25 clinical HRPCs and 10 hormone-sensitive prostate cancers (HSPCs) by genome-wide cDNA microarrays combining with laser microbeam microdissection. An unsupervised hierarchical clustering analysis clearly distinguished expression patterns of HRPC cells from those of HSPC cells. In addition, primary and metastatic HRPCs from three patients were closely clustered regardless of metastatic organs. A supervised analysis and permutation test identified 36 up-regulated genes and 70 down-regulated genes in HRPCs compared with HSPCs (average fold difference > 1.5; P < 0.0001). We observed overexpression of AR, ANLN, and SNWPE and down-regulation of NR4A1, CYP27A1, and HLA-A antigen in HRPC progression. AR overexpression is likely to play a central role of hormone-refractory phenotype, and other genes we identified were considered to be related to more aggressive phenotype of clinical HRPCs, and in fact, knockdown of these overexpressing genes by small interfering RNA resulted in drastic attenuation of prostate cancer cell viability. Our microarray analysis of HRPC cells should provide useful information to understand the molecular mechanism of HRPC progression and to identify molecular targets for development of HRPC treatment.

    DOI: 10.1158/0008-5472.CAN-06-4040

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  • Suicide gene therapy with adenoviral delivery of HSV-tK gene for patients with local recurrence of prostate cancer after hormonal therapy 査読

    Nasu Yasutomo, Saika Takashi, Ebara Shin, Kusaka Nobuyuki, Kaku Haruki, Abarzua Fernando, Manabe Daisuke, Thompson Timothy C, Kumon Hiromi

    MOLECULAR THERAPY   15 ( 4 )   834 - 840   2007年4月

  • Comparative study of oncologic outcome of laparoscopic nephroureterectomy and standard nephroureterectomy for upper urinary tract transitional cell carcinoma 査読

    Daisuke Manabe, Takashi Saika, Shin Ebara, Shinya Uehara, Atsushi Nagai, Ryuji Fujita, Shin Irie, Daisuke Yamada, Tomoyasu Tsushima, Yasutomo Nasu, Hiromi Kumon

    UROLOGY   69 ( 3 )   457 - 461   2007年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    OBJECTIVES To determine the oncologic safety of laparoscopic nephroureterectomy (LNU), we compared the long-term oncologic outcome of LNU versus open nephroureterectomy (ONU) in patients with upper tract transitional cell carcinoma.
    METHODS A total of 367 nephroureterectomy procedures were performed at our institutes for upper tract transitional cell carcinoma without distant metastases. Of 224 patients without concomitant or previous bladder cancer, 58 underwent LNU with open intact specimen retrieval plus open distal ureter and bladder cuff removal and 166 underwent ONU. Their data were reviewed and analyzed retrospectively. The mean follow-up was 13.6 months (range 14 to 34) for the LNU group and 28.0 months (range 14 to 36) for the ONU group.
    RESULTS Bladder recurrence was recognized in 19 patients (32.8%) after LNU at a median follow-up of 5.6 months compared with 63 patients (38.0%) after ONU. Local recurrence only developed in 2 patients (1.1%) after ONU. One port site metastasis occurred in a patient who had undergone LNU. Distant metastases developed in 10 patients (17.2%) after LNU and 33 patients (19.9%) after ONU. The frequency of bladder recurrence, local recurrence, and distant metastases did not differ significantly between the two groups. The actual disease-free 2-year survival rates were similar (75.6% versus 81.7%). In all patients, the risk of metastases and death increased with advanced tumor stage and grade, but not by surgical procedure.
    CONCLUSIONS In the surgical management of upper tract transitional cell carcinoma, LNU does not negatively affect long-term oncologic control and can be considered an alternative modality. Tumor stage and grade are, however, important prognostic factors in the incidence of metastases and cancer-specific mortality.

    DOI: 10.1016/j.urology.2006.11.005

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  • REIC/Dkk-3 as a potential gene therapeutic agent against human testicular cancer 査読

    Ryuta Tanimoto, Fernando Abarzua, Masakiyo Sakaguchi, Mikiro Takaishi, Yasutomo Nasu, Hiromi Kumon, Nam-Ho Huh

    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE   19 ( 3 )   363 - 368   2007年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PROFESSOR D A SPANDIDOS  

    Human testicular cancer is very sensitive to chemotherapy and radiation therapy and is regarded as a curable cancer. The cancer prevails in the young reproductive generation and testicular dysfunction is often observed as a side effect, remaining a serious challenge. In the present study, we examined the potential utility of REIC/Dkk-3-based gene therapy against human testicular cancer. Expression of REIC/Dkk-3 was reduced in all of the human seminoma and non-seminomatous germ cell tumor tissues. Overexpression of REIC/Dkk-3 using an adenovirus vector (Ad-REIC) induced apoptosis in a testicular germ cell cancer cell line NCCIT but not in normal human fibroblasts. c-Jun terminal kinase (JNK) was activated by Ad-REIC and the induction of apoptosis was abrogated by a JNK inhibitor. A single intratumoral injection of Ad-REIC markedly inhibited the tumorigenic growth of NCCIT cells in nude mice. These results indicate that Ad-REIC may lead to developing less insulting and non-genotoxic therapeutic measures against human testicular cancer.

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  • テロメラーゼ特異的複製:前立腺癌に対する選択的ウイルス療法(Telomerase-Specific Replication: Selective Virotherapy for Prostate Cancer) 査読

    黄 鵬, 渡部 昌実, 陳 潔, 賀来 春紀, 那須 保友, 雑賀 隆史, 藤原 俊義, 浦田 泰生, 公文 裕巳

    日本泌尿器科学会雑誌   98 ( 2 )   458 - 458   2007年2月

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    記述言語:英語   出版者・発行元:(一社)日本泌尿器科学会  

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  • Primary aldosteronism caused by a unilateral adrenal adenoma accompanied by autonomous cortisol secretion

    Suzuki J, Otsuka F, Inagaki K, Otani H, Miyoshi T, Terasaka T, Ogura T, Omori M, Nasu Y, Makino H

    Hypertens Res   30 ( 4 )   367 - 373   2007年

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    記述言語:英語  

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  • Implication of amphiphysin 1 and dynamin 2 in tubulobulbar complex formation and spermatid release

    Kusumi N, Watanabe M, Yamada H, Li S, Kashiwakura Y, Matsukawa T, Nagai A, Nasu Y, Kumon H, Takei K

    Cell Structure and Function   32   101 - 113   2007年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Phase study of adenoviral vector delivery of HSV-tk gene and the intravenous administration of ganciclovir in men with local recurrence of prostate cancer after hormonal therapy 査読

    Nasu Yasutomo, Kaku Haruki, Ebara Shin, Saika Takashi, Thompson Timothy C, Kumon Hiromi

    JOURNAL OF GENE MEDICINE   8 ( 12 )   1443   2006年12月

  • Telomerase-specific replication-selective virotherapy for prostate cancer 査読

    Kaku Haruki, Nasu Yasutomo, Huang Peng, Saika Takashi, Kagawa Shunsuke, Fujiwara Toshiyoshi, Kumon Hiromi, Urata Yasuo

    JOURNAL OF GENE MEDICINE   8 ( 12 )   1454   2006年12月

  • Phase I/II Study of Adenoviral Vector Delivery of HSV-tk Gene and the Intravenous Administration of Ganciclovir in Men with Local Recurrence of Prostate Cancer after Hormonal Therapy 査読

    Nasu Yasutomo, Kaku Haruki, Ebara Shin, Saika Takashi, Kumon Hiromi, Thompson Timothy C

    MOLECULAR THERAPY   13   S19 - S20   2006年5月

  • Effects of radiofrequency ablation on individual renal function: Assessment by technetium-99m mercaptoacetyltriglycine renal scintigraphy 査読

    T Mukai, S Sato, T Iguchi, H Mimura, K Yasui, H Gobara, T Saika, Y Nasu, H Kumon, S Kanazawa

    ACTA MEDICA OKAYAMA   60 ( 2 )   85 - 91   2006年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We quantitatively evaluated total and individual renal function by technetium-99m mercaptoacetyltriglycine (Tc-99m MAG3) renal scintigraphy before and after radiofrequency ablation (RFA) of renal tumors. Eleven patients who underwent Tc-99m MAG3 renal scintigraphy I week before and after RFA were evaluated (7 men and 4 women; age range: 23-83 years; mean age: 60.6 years). Five patients had solitary kidneys, and five had normally or minimally functioning contralateral kidneys. One patient had a renal cell carcinoma in the contralateral. kidney. One patient with a solitary kidney underwent RFA a second time for a residual tumor. In patients with a solitary kidney, MAG3 clearance decreased after 5 of 6 RFAs, and in patients with a normally functioning contralateral kidney, MAG3 clearance decreased after 4 of 5 RFAs, but no significant differences were observed between before and after treatments. In addition to the total MAG3 clearance, the split MAG3 clearance was evaluated in patients with a normally functioning contralateral. kidney. MAG3 clearance decreased in 4 of 5 treated kidneys, while it adversely increased in the contralateral kidneys after 4 of 5 RFAs. No significant differences, however, were observed between before and after treatments. The results of our study revealed no significant differences in sCr, BUN, CCr, or MAG3 clearance between pre- and post-RFA values. These results support data regarding the functional impact and safety of renal RFA in published reports. We evaluated total and individual renal function quantitatively using Tc-99m MAG3 renal scintigraphy before and after treatment. This scintigraphy was very useful in assessing the effects of RFA on renal function.

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  • Time course of serum testosterone and luteinizing hormone levels after cessation of long-term luteinizing hormone-releasing hormone agonist treatment in patients with prostate cancer 査読

    H Kaku, T Saika, T Tsushima, S Ebara, T Senoh, T Yamato, Y Nasu, H Kumon

    PROSTATE   66 ( 4 )   439 - 444   2006年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    INTRODUCTION. In order to elucidate the influence of hormone-releasing hormone (LHRH) agonist therapy cessation on pituitary/testicular function and its clinical implications, we investigated prospectively hormonal (luteinizing hormone: LH; testosterone: T) responses in patients with prostate cancer who received long-term LH-RH 10 agonist therapy.
    PATIENTS AND METHODS. A consecutive 32 patients who had received LH-RH agonist therapy over 24 months were enrolled. As a baseline, T and LH were measured at the time of LHRH agonist therapy cessation, monthly for 3 months, and subsequently, every 3 months.
    RESULTS. The median duration of LH-RH agonist therapy was 30 months (24-87 months) with median follow-up duration of 24 months following cessation. All patients had castrated T levels and suppressed LH levels at baseline. Median duration of castrated T levels following cessation was 6 months. Median time to normalization of T levels was 24 months. LH levels returned to normal within 3 months in all cases. Patients who received androgen deprivation therapy for 30 months or longer required a longer time for recovery of T levels. Patients over 65 years of age showed a statistically significant longer time for recovery of T levels (P = 0.0167).
    CONCLUSIONS. Long-term LH-RH agonist therapy has remarkable effects on serum T level that last for a significant time after cessation, a fact that should be applied to the interpretation of both PSA and serum T levels after cessation of androgen deprivation therapy.

    DOI: 10.1002/pros.20341

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  • Combination chemotherapy with estramustine phosphate, ifosfamide and cisplatin for hormone-refractory prostate cancer 査読

    H Kaku, T Saika, T Tsushima, A Nagai, T Yokoyama, F Abarzua, S Ebara, D Manabe, Y Nasu, H Kumon

    ACTA MEDICA OKAYAMA   60 ( 1 )   43 - 49   2006年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We evaluated the efficiency and toxicity of estramustine phosphate (ECT), ifosfamide (IFM) and cisplatin (CDDP) combination chemotherapy in twenty-one patients with hormone-refractory prostate cancer (HRPC), for which there is currently no effective treatment. Patients received a daily dose of 560 mg ECT in combination with 1.2 g/m(2) IFM on days 1 to 5 and 70 mg/m(2) CDDP on day 1. This combination therapy was given every 3 to 4 weeks. An objective response of more than 50% reduction in prostate-specific antigen was observed in 9 of 18 patients (50% and a more than 50% reduction in bi-dimensionally measurable soft-tissue lesions was observed in 2 of 7 patients (29%). The median duration of response among the cases showing partial response was 40 weeks, while the median duration of response of overall partial-response plus stable cases was 30 weeks. The median survival duration of all cases was 47 weeks. Toxicity was modest and acceptable. In conclusion, the ECT, IFM and CDDP combination chemotherapy regimen is a viable treatment option for HRPC. However, in comparison with our previous chemotherapy regimen of IFM and CDDP, no additional long-lasting effects resulting from the inclusion of ECT could be affirmed.

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  • Induction of cell death in human papillomavirus 18-positive cervical cancer cells by E6 siRNA

    Yamato K, Fen J, Kobuchi H, Nasu Y, Yamada T, Nishihara T, Ikeda Y, Kizaki M, Yoshinouchi M

    Cancer Gene Therapy   13   234 - 241   2006年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Comparison of 25 and 75 mg/day naftopidil for lower urinary tract symptoms associated with benign prostatic hyperplasia: a prospective, randomized controlled study.

    Yokoyama T, Kumon H, Nasu Y, Takamoto H, Watanabe T

    Int J Urol   13 ( 7 )   932 - 938   2006年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • The Jounal of gene medicine Japanese society of gene therapy young investigator award 2006

    Nasu Y

    The Journal of Gene Medicine   8   1363 - 1364   2006年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Hepsin shows inhibitory effects through apoptotic pathway on ovarian cancer cell lines

    Nakamura K, Nasu Y, Hongo A, Matsuo T, Kodama J, Ebara S, Nagai A, Abrzua F, Kumon H, Hiramatsu Y

    International Journal of Oncology   28   393 - 398   2006年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Intracavernous injection of prostaglandin E-1 is effective in patients with erectile dysfunction not responding to phosphodiesterase 5 inhibitors 査読

    A Nagai, N Kusumi, H Tsuboi, K Ishii, T Saika, Y Nasu, H Kumon

    ACTA MEDICA OKAYAMA   59 ( 6 )   279 - 280   2005年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We report on 64 patients who did not achieve erections adequate for satisfactory sexual intercourse from among a total of 243 patients who were prescribed PDE5 inhibitors for erectile dysfunction (ED). Intracavernous injection (ICI) of PGE(1), was performed in this non-responder group. An ICI of 20 or 40 mcg of PGE(1) in 1 ml saline was performed and the responses evaluated. Forty-nine out of 64 (77%) cases responded to 20 mcg of PGE(1). Forty mcg of PGE(1) was injected into the 15 non-responding cases, and 9 patients responded favorably. The overall effective rate was 58/64 (91 %). No major adverse effects were observed.

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  • Adenovirus-mediated overexpression of REIC/Dkk-3 selectively induces apoptosis in human prostate cancer cells through activation of c-Jun-NH2-kinase 査読

    F Abarzua, M Sakaguchi, M Takaishi, Y Nasu, K Kurose, S Ebara, M Miyazaki, M Namba, H Kumon, N Huh

    CANCER RESEARCH   65 ( 21 )   9617 - 9622   2005年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC CANCER RESEARCH  

    Alteration in genes which takes place during malignant conversion and progression could be potential targets for gene therapy. We previously identified REIC/Dkk-3 as a gene whose expression is reduced in many human cancers. Here, we showed that expression of REIC/Dkk-3 was consistently reduced in human prostate cancer tissues in a stage-dependent manner. Forced expression of REIC/Dkk-3 induced apoptosis in human prostate cancer cell lines lacking endogenous REIC/Dkk-3 expression but not in REIC/Dkk3-proficient normal prostate epithelial and stromal cells. The apoptosis involved c-jun-NH2-kinase activation, mitochondrial translocation of Bax, and reduction of Bcl-2. A single injection of an adenovirus vector carrying REIC/Dkk-3 showed a dramatic antitumor effect on a xenotransplanted human prostate cancer. Thus, REIC/Dkk-3 could be a novel target for gene-based therapy of prostate cancer.

    DOI: 10.1158/0008-5472.CAN-05-0829

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  • Long-term clinical outcomes of 420 consecutive prostate cancer patients in a single institute 査読

    K Edamura, T Saika, T Senoh, F Koizumi, D Manabe, S Ebara, H Kaku, T Yokoyama, F Abarzua, A Nagai, Y Nasu, T Tsushima, H Kumon

    ACTA MEDICA OKAYAMA   59 ( 5 )   195 - 199   2005年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    This study was undertaken to reveal the trends of prostate cancer and the outcome of treatment modalities for each disease stage in patients in a single institute over a 10-year period. From January 1994 through December 2003, 420 consecutive patients with previously untreated and histologically confirmed prostate cancer were analyzed for annual distributions of disease stages and treatment modalities and for long-term clinical progression-free survival, prostate cancer-specific survival, and prostate-specific antigen (PSA) failure-free survival rates for each stage and treatment modality. Annual trends showed that the number of patients, especially those with clinically localized cancer, increased dramatically. The 5-year disease-specific survival rates for patients with clinically localized disease were 100% for all treatment modalities, including hormonal therapy alone. Patients with PSA levels less than 10 ng/ml showed an 81% 5-year PSA failure-free survival rate with radical prostatectomy. Stage C patients treated by surgery or radiation-based therapy with concomitant hormonal therapy obtained 93% and 100% cause-specific survival rates, respectively, and those treated by hormonal therapy alone showed a 79% rate. The number of patients with localized prostate cancer was increasing in this decade. While long-term hormonal therapy alone was highly efficient in controlling localized prostate cancer, radical therapies in conjunction with neo-adjuvant hormonal therapy produced better survival rates in cases of locally advanced disease.

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  • Clinical results of one-stage urethroplasty with parameatal foreskin flap for hypospadias 査読

    A Nagai, Y Nasu, M Watanabe, N Kusumi, H Tsuboi, H Kumon

    ACTA MEDICA OKAYAMA   59 ( 2 )   45 - 48   2005年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    We investigated the usefulness of one-stage urethroplasty by the parameatal foreskin flap method (OUPF procedure), which is useful for repairing all types of hypospadias. Between June 1992 and March 2001, the OUPF procedure was performed on 18 patients with hypospadias: 10 patients with distal and 8 with proximal hypospadias. The follow-up periods ranged from 33-75 months, with an average of 52 months. The duration of surgery, the catheter indwelling period, and the postoperative complications of each patient were analyzed. The median age of the patients at the time of surgery was 3 years and 8 months. The length of surgery for OUPF II ranged from 150-230 min (average 186 min), and from 190-365 min (average 267 min) for OUPF IV. Postoperative complications were confirmed in 3 of the 18 patients (16.6%). Two patients had fistulas, and one had a meatal regression. The fistulas were successfully closed by the simple multilayered closure method. After adopting DuoDerm dressings instead of elastic bandages for protection of the wound, no fistulization occurred. DuoDerm dressings are useful in the healing of wounds without complications. To date, the longest follow-up period has been 75 months, and during that time there have been no late complications such as urethral stenosis or penile curvature. OUPF is a useful method in the treatment of hypospadias with a low incidence of early and late complications.

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  • Predicting postoperative renal insufficiency in patients undergoing nephrectomy for renal malignancy: Assessment by renal scintigraphy using (99m)Technetium-mercaptoacetyltriglycine 査読

    Y Shirasaki, T Saika, T Tsushima, Y Nasu, R Arata, H Kumon

    JOURNAL OF UROLOGY   173 ( 2 )   388 - 390   2005年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Purpose: We performed 99(m)Tc-mercaptoacetyltriglycine (MAG3) renal scintigraphy in patients with renal malignancy to evaluate the function of each renal unit before and after nephrectomy to see if postoperative functional deterioration could be predicted based on scintigraphy results and creatinine clearance.
    Materials and Methods: A total of 22 men and 13 women with renal malignancy, including 32 with renal cell carcinoma and 3 with urothelial cancer, were prospectively enrolled in this study. Average patient age was 64.3 years (median 65, range 43 to 88). All patients underwent MAG3 renal scintigraphy before and after unilateral nephrectomy. At the same time serum creatinine and endogenous creatinine clearance were determined.
    Results: Mean serum creatinine was 0.93 mg/dl before and 1.31 after nephrectomy (p <0.0001). Preoperative endogenous creatinine clearance was 70.8 ml per minute per 1.73 m(2), which decreased to 49.0 ml per minute per 1.73 m(2) after nephrectomy (p <0.0001). Mean MAG3 clearance of the remaining kidney increased 35.1% above baseline from 156.5 to 211.5 ml per minute per 1.73 m(2) following nephrectomy. Spearman rank core analysis revealed that preoperative MAG3 clearance of the remaining kidney significantly correlated with postoperative creatinine clearance (r = 0.596, p = 0.0005). Preoperative MAG3 clearance of the remaining kidney more than 130 ml per minute per 1.73 M-2 coincided with postoperative creatinine clearance above 40 ml per minute per 1.73 m(2).
    Conclusions: MAG3 renal scintigraphy may be useful for predicting renal insufficiency after nephrectomy. The findings in this study suggest that preoperative MAG3 clearance of the remaining kidney less than 130 ml per minute per 1.73 m(2) is a risk factor for postoperative renal insufficiency.

    DOI: 10.1097/01.ju.0000149980.61593.5f

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  • Varicocele rupture due to sexual intercourse

    Nishiyama Y, Nagai A, Nasu Y, Watanabe M, Kusumi N, Monden K, Kumon H

    Int J Urol   12   585 - 587   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Sex reassignment surgery for male to female transsexuals:Initial experience at Okayama University Hospital

    Nagai A, Tokuyama E, Nanba Y, Tsutsui T, Kimata Y, Nakatsuka M, Koshima I, Saika T, Nasu Y, Kumon H

    Acta Med. Okayama   59 ( 5 )   231 - 233   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Adeno-associated virus-mediated human IL-10 gene transfer suppresses the development of experimental autoimmune orchitis

    Watanabe M, Kashiwakura Y, Kusumi N, Tamayose K, Nasu Y, Nagai A, Shimada T, Daida H, Kumon H

    Gene Therapy   12   1126 - 1132   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Minimal invasiveness and effectivity of subinguinal microscopic varicocelectomy:A comparative study with retroperitoneal high and laparoscopic approaches

    Watanabe M, Nagai A, Kusumi N, Tsuboi H, Nasu Y, Kumon H

    Int J Urol   12   892 - 898   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Transrectal high-intensity focused ultrasound in the treatment of localized prostate cancer:A multicenter study

    Uchida T, Baba S, Irie A, Soh S, Masumori N, Tsukamoto T, Nakatsu H, Fujimoto H, Kakizoe T, Ueda T, Ichikawa T, Ohta N, Kitamura T, Sumitomo M, Hayakawa M, Aoyagi T, Tachibana M, Ikeda R, Suzuki K, Tsuru N, Suzuki K, Ozono S, Fujimoto K, Hirao Y, Monden K, Nasu Y, Kumon H, Nishi K, Ueda S, Koga H, Naitoh S

    Acta Urol Jpn   51 ( 10 )   651 - 658   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Analysis of human ejaculation using color doppler ultrasonography:A comparison between antegrade and retrograde ejaculation

    Nagai A, Watanabe M, Nasu Y, Iguchi H, Kusumi N, Kumon H

    Urology   65 ( 2 )   365 - 368   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Adeno-Associated Virus 2-Mediated intratumoral prostate cancer gene therapy:Long-term maspin expression efficiently suppresses tumor growth

    Watanabe M, Nasu Y, Kashiwakura Y, Kusumi N, Tamayose K, Nagai A, Sasano T, Shimada T, Daida H, Kumon H

    Human Gene Therapy   16   699 - 710   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • XAGE-1 rnRNA expression in prostate cancer and antibody response in patients 査読

    F Koizumi, Y Noguchi, T Saika, K Nakagawa, S Sato, AMA Eldib, Y Nasu, H Kumon, E Nakayama

    MICROBIOLOGY AND IMMUNOLOGY   49 ( 5 )   471 - 476   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    To evaluate the feasibility of cancer vaccine targeting XAGE-1, we investigated the expression of 4 XAGE-1 transcript variants and the humoral immune response to XAGE-1 in prostate cancer patients. XAGE-1a, b, c, d mRNA expression was analyzed in 54 prostate cancer specimens and 8 specimens of benign prostate hypertrophy (BPH) by reverse transcription-polymerase chain reaction (RT-PCR). The Immoral response to XAGE-1 was investigated in sera obtained from 278 patients with prostate cancer and 40 healthy volunteers by enzyme-linked immunosorbent assay (ELISA) using recombinant protein. XAGE-1b mRNA expression was observed in 14 of 54 (26%) prostate cancer specimens, while XAGE-1a, c, and d mRNA expressions were observed in 1, 1, and 3, respectively. None of the 4 XAGE-1 transcript variants was observed in the 8 BPH specimens. Antibody against XAGE-1 was detected in sera from 2 of 129 stage D2 patients, whereas none of sera from 149 patients with localized prostate cancer or lymph node metastasis had detectable XAGE-1 antibody. No reactivity to XAGE-1 was found in sera from the 40 healthy individuals.

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  • Long-term consequence of renal function following nephrectomy for renal cell cancer 査読

    Y Shirasaki, T Tsushima, Y Nasu, H Kumon

    INTERNATIONAL JOURNAL OF UROLOGY   11 ( 9 )   704 - 708   2004年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING ASIA  

    Aim: A retrospective analysis was performed involving patients who had undergone radical nephrectomy for renal cell cancer to determine the long-term outcome of this surgery on renal status.
    Materials and Methods Between 1977 and 2001, 172 patients having undergone radical nephrectomy for renal cell cancer were followed for a period of more than one year at the Department of Urology Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan. The preoperative and postoperative serum creatinine values of these individuals were reviewed. Long-term effects of radical nephrectomy on renal function and factors influencing postoperative renal dysfunction were analyzed.
    Results: Six (3.5%) of the 172 individuals progressed to hemodialysis. Eleven (6.4%) patients displayed serum creatinine values of greater than or equal to1.6 mg/dL. The remaining 155 patients (90.1%) demonstrated postoperative serum creatinine values of <1.6 mg/dL. The present study suggests that aging, a high degree of proteinuria, hypertension and diabetes mellitus were the predominant risk factors for renal deterioration following nephrectomy.
    Conclusions: We have primarily selected radical nephrectomy for cases exhibiting smaller renal tumors with a normal contralateral kidney. We encourage nephron-sparing surgery where possible in patients displaying risk factors, including a high degree of proteinuria, hypertension, or diabetes mellitus.

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  • Positive correlation between allelic loss at chromosome 14q24-31 and poor prognosis of patients with renal cell carcinoma 査読

    H Kaku, S Ito, S Ebara, M Ouchida, Y Nasu, T Tsushima, H Kumon, K Shimizu

    UROLOGY   64 ( 1 )   176 - 181   2004年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Objectives. To report our development of a new application of the inter-AN long polymerase chain reaction (PCR) for genomic scanning to screen for tumor-specific alterations in tumor DNA. Using this method, we detected a rearranged chromosomal region in renal cell carcinomas (RCCs). We then examined tumor-specific allelic loss in this region using microsatellite markers and determined whether a relationship was present between this allelic loss and the clinicopathologic features of the patients.
    Methods. The inter-Alu long PCR genomic scan method was performed using RCC DNA samples and primers specific for a minor subset of the human repeat sequence Alu. We analyzed DNA samples from 42 pairs of matched normal and nonpapillary RCC tissues with seven microsatellite markers.
    Results. The inter-Alu long PCR genomic scan method revealed an altered DNA region on chromosome 14q24-31, which is the location of several putative tumor suppressor genes. At least one of seven microsatellite markers on chromosome 14q24-31 showed loss of heterozygosity in 23 (54.8%) of 42 informative cases of RCC. The prevalent loss region was confined to a 2-Mb region around D14S67. We found a positive correlation between the presence of the loss of heterozygosity on 14q24-31 and tumor stage (P < 0.05). We also found that cases with allelic loss at 14q24-31 had a poor prognosis (P = 0.045).
    Conclusions. Our inter-Alu long PCR genomic scan method is a powerful method for the screening of DNA alterations, and our data suggest that the chromosome 14q24-31 region contains likely tumor suppressor genes associated with the progression of RCC. (C) 2004 Elsevier Inc.

    DOI: 10.1016/j.urology.2004.03.015

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  • Effects of conadotropin-releasing hormone agonists on bone metabolism markers and bone mineral density in patients with prostate cancer 査読

    Y Miyaji, T Saika, Y Yamamoto, N Kusaka, R Arata, S Ebara, Y Nasu, T Tsushima, H Kumon

    UROLOGY   64 ( 1 )   128 - 131   2004年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Objectives. To better understand bone metabolism and predict bone loss in treatment using gonadotropin-releasing hormone agonist for patients with prostate cancer.
    Methods. The changes in bone mineral density and blood levels of bone metabolism markers and the level of pyridinoline cross-linked carboxy-terminal telopeptide of type I collagen, a specific marker of bone resorption, and carboxy-terminal pro-peptide of human type I procollagen, a specific marker of bone formation, were examined in 27 consecutive patients with prostate cancer without bone metastasis.
    Results. After 2 years of gonadotropin-releasing hormone treatment, the bone mineral density was significantly lower (median 0.937 g/cm(2)) than before treatment. Pyridinoline cross-linked carboxy-terminal telopeptide of type I collagen began to increase significantly 6 months after the start of treatment (3.0 to 8.3 ng/mL, median 4.6, at baseline versus 3.4 to 8.2 ng/mL, median 5.2, after 6 months). Carboxy-terminal pro-peptide of human type I procollagen began to show a significant rise I year after the start of treatment (from 72.8 to 221.5 ng/mL, median 102.0, at baseline to 82.7 to 293.4 ng/mL, median 132.0, at I year).
    Conclusions. Functional coupling between bone resorption and formation was noted, and a decrease in bone mass, even in men, owing to androgen deficiency, was biochemically demonstrated. Fluctuations in these two bone metabolism markers preceded the decrease of bone mineral density. Therefore, these markers might be a predictor of bone loss. (C) 2004 Elsevier Inc.

    DOI: 10.1016/j.urology.2004.03.012

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  • Kidney function after nephrectomy for renal cell carcinoma 査読

    Y Shirasaki, T Tsushima, T Saika, Y Nasu, H Kumon

    UROLOGY   64 ( 1 )   43 - 47   2004年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Objectives. To evaluate the function of the remaining kidney after nephrectomy for renal cell cancer by technetium-99m-mercaptoacetyltriglycine (Tc-99m-MAG3) renal scintigraphy.
    Methods. We evaluated 30 consecutive patients who were undergoing unilateral radical nephrectomy by Tc-99m-MAG3 scintigraphy. All patients underwent three consecutive 99mTc-MAG3 scintigraphy studies. The first study was performed before nephrectomy, the second 1 month after surgery, and the third 1 year after surgery. At these times, the serum creatinine levels were also evaluated.
    Results. The mean preoperative MAG3 clearance of the remaining kidney of the 30 patients was 155.4 mL/min/1.73 m(2). The mean MAG3 clearance of the remaining kidney had increased to 209.2 mL/min/1.73 m(2) by I month after nephrectomy, and the average percentage increase was 39.5%. After 1 year, it had increased to 211.3 mL/min/1.73 m(2), with a 40.5% average percentage increase. The preoperative MAG3 clearance of the remaining kidney was inversely correlated with the percentage of increase in MAG3 clearance of the remaining kidney. Abnormal serum creatinine levels (greater than 1.3 mg/dL) were more common after nephrectomy, occurring in 6 patients at 1 month and in 5 patients at 1 year postoperatively. In all 6 patients with elevated creatinine levels, the preoperative MAG3 clearance of the remaining kidney was less than 150 mL/min/1.73 m(2).
    Conclusions. Adaptive hyperfunction occurs soon after nephrectomy that is not associated with age or sex and continues for at least 1 year. A greater compensatory response is produced in patients with more severe renal deterioration. Using (99)mTc-MAG3 scintigraphy, we may be able to predict postoperative renal function. (C) 2004 Elsevier Inc.

    DOI: 10.1016/j.urology.2004.02.039

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  • Comparative study of ureteral stripping versus open ureterectomy for nephroureterectomy in patients with transitional carcinoma of the renal pelvis 査読

    T Saika, J Nishiguchi, T Tsushima, Y Nasu, A Nagai, Y Miyaji, Y Maki, T Akaeda, M Saegusa, H Kumon

    UROLOGY   63 ( 5 )   848 - 852   2004年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Objectives. To evaluate the clinical outcome of nephroureterectomy with endoscopically assisted transurethral ureteral stripping for transitional cell carcinoma of the renal pelvis in a comparative study.
    Methods. Sixty patients with localized renal pelvic cancer were enrolled in a prospective comparative nonrandomized study. Of these, 28 patients underwent nephroureterectomy with endoscopically assisted transurethral ureteral stripping and 32 underwent conventional nephroureterectomy with a bladder cuff. Both short-term and long-term results were analyzed in this series.
    Results. The operating time for patients with ureteral stripping was significantly shorter than for those with a standard two-incision nephroureterectomy (median 183 versus 250 minutes, P = 0.0231), and the amount of blood loss was significantly less (median 150 versus 390 mL, P = 0.0002). Intravesical recurrence was detected in 10 (35.7%) of the 28 patients with ureteral stripping, and the 1-year and 3-year recurrence-free rate was 68.0% and 57.7%, respectively. Seven patients treated by the standard two-incision nephroureterectomy (21.9%) experienced intravesical recurrence, with a 1-year and 3-year recurrence-free rate of 96.8% and 75.0%, respectively. The recurrence rate was significantly greater in the group with ureteral stripping (P = 0.0287).
    Conclusions. Compared with conventional nephroureterectomy with a bladder cuff, nephroureterectomy with transurethral stripping is a minimally invasive procedure with a shorter operating time and less blood loss, but a statistically significantly greater intravesical recurrence rate. Greater consideration should be taken before selecting this procedure. (C) 2004 Elsevier Inc.

    DOI: 10.1016/j.urology.2003.12.003

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  • Decreased expression of REIC/Dkk-3 in human renal clear cell carcinoma

    Kurose K, Sakaguchi M, Nasu Y, Ebara S, Kaku H, Kariyama R, Arao Y, Miyazaki M, Tsushima T, Namba M, Kumon H, Huh N

    J Urol   171   1314 - 1318   2004年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Analysis of retrograde ejaculation using color Doppler ultrasonography before and after transurethral collagen injection

    Nagai A, Nasu Y, Watanabe M, Tsugawa M, Iguchi H, Kumon H

    International Journal of Impotence Research   16   456 - 458   2004年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Ureteroscopy using a detachable access sheath

    Abarazua F, Monden K, Nagai A, Nasu Y, Kumon H

    Acta Med. Okayama   58 ( 4 )   215 - 216   2004年

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    記述言語:英語   掲載種別:研究論文(大学,研究機関等紀要)  

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  • Dynamin-2 regulates oxidized low-density lipoprotein-induced apoptosis of vascular smooth muscle cell.

    Kashiwakura Y, Watanabe M, Kusumi N, Sumiyoshi K, Nasu Y, Yamada H, Sawamura T, Kumon H, Takei K, Daida H

    Circulation   110 ( 21 )   3329 - 3334   2004年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Anterior urethral recurrence of superficial bladder cancer: Its clinical significance 査読

    T Saika, T Tsushima, Y Nasu, R Arata, H Kaku, N Akebi, N Kusaka, H Kumon

    ACTA MEDICA OKAYAMA   57 ( 6 )   293 - 297   2003年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    The aim of this study was to reveal the clinical features of anterior urethral recurrence in patients with superficial bladder cancer, and to determine the appropriate treatment. Three hundred and three patients with superficial bladder cancer, who were newly diagnosed and initially treated conservatively in our hospital between 1965 and 1990, were followed for at least 5 years and their clinical outcomes were analyzed. Clinical factors, including anterior urethral recurrence, were evaluated statistically regarding tumor progression. Eight patients (2.6%) had anterior urethral recurrence following superficial bladder cancer. Twenty-four patients (7.9%) had tumor progression and 149 (49.2%) had tumor recurrence. In a multivariate analysis using a logistic model, anterior urethral recurrence was the most important factor, followed by histological grade. Four of 5 patients who were treated for anterior urethral recurrent tumors by transurethral resection showed progression and died of the cancer within one year. Two of the remaining three patients who underwent radical cysto-urethrectomy at the time of anterior urethral recurrence survived. Anterior urethral recurrence following superficial bladder cancer is a predictor for rapid subsequent malignant progression. Once there is anterior urethral recurrence, radical intensive therapy, including radical cysto-urethrectomy, should be carried out immediately.

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  • NY-ESO-1 mRNA expression and immunogenicity in advanced prostate cancer 査読

    Tetsuya Nakada, Yuji Noguchi, Shuichiro Satoh, Toshiro Ono, Takashi Saika, Takushi Kurashige, Sacha Gnjatic, Gerd Ritter, Yao-Tseng Chen, Elisabeth Stockert, Yasutomo Nasu, Tomoyasu Tsushima, Hiromi Kumon, Lloyd J. Old, Eiichi Nakayama

    Cancer Immunity   3   1 - 12   2003年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Academy of Cancer Immunology  

    NY-ESO-1 mRNA expression was investigated in advanced prostate cancer by conventional and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). NY-ESO-1 mRNA was detected in 20 of 53 (38%) tumor specimens. Four of 15 (27%) stage C, 1 of 3 stage D1 (33%) and 15 of 35 (43%) stage D2 prostate cancers were positive. The presence of NY-ESO-1 antibodies was evaluated in sera from a panel of 218 patients with prostate cancer, including the 53 patients whose tumors were examined for NY-ESO-1 mRNA expression. NY-ESO-1 antibodies were detected in 1 of 30 (3.3%) stage D1 and 9 of 110 (8.2%) stage D2 patients, whereas none of the 78 patients with localized prostate cancer (stages A, B and C) had detectable NY-ESO-1 antibodies. Of the 53 patients whose tumors were examined for NY-ESO-1 mRNA expression, 2 of 20 patients with NY-ESO-1 mRNA-positive tumors had NY-ESO-1 antibodies. No antibody was found in the sera of 32 patients with NY-ESO-1 mRNA-negative tumors, with the exception of one patient with regional lymph node metastasis (stage D1). CD8 T cell responses specific to NY-ESO-1 were detected in two of three patients with NY-ESO-1 antibodies.

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  • Laparoscopic removal of mullerian structures and orchiopexy for persistent mullerian duct syndrom

    Shirasaki Y, Nagai A, Nasu Y, Iguchi H, Kumon H

    Urology   62 ( 6 )   1121   2003年

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    記述言語:英語  

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  • Clinical study of G3 superficial bladder cancer without concomitant CIS treated with conservative therapy 査読

    T Saika, T Tsushima, Y Nasu, R Arata, H Kaku, N Kusaka, H Kumon

    JAPANESE JOURNAL OF CLINICAL ONCOLOGY   32 ( 11 )   461 - 465   2002年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Objective: The treatment for superficial G3 transitional cell carcinoma (TCC) of the urinary bladder remains controversial. It is important to reveal the clinical features of superficial G3 bladder cancer that can be treated conservatively.
    Patients and Methods: A total of 39 patients with primary superficial bladder cancer (Ta, T1) with G3 components but without concomitant carcinoma in situ (CIS), who had been treated initially with transurethral resection (TUR), were retrospectively analyzed for factors related to tumor recurrence, progression and survival. The patients were 34 males and five females who,se age ranged from 49 to 85 years (average, 68 years). Initial tumor stages were Ta in one patient and T1 in 38. Initial treatments were TUR alone in 18 patients and TUR with adjuvant therapy (intravesical chemotherapy or BCG therapy) in 21. Factors examined included age, gender, morphology, size and number of tumors and adjuvant therapies.
    Results: Follow-up periods were 3-138 months (median, 37 months). Tumor recurrence, progression and cancer death were observed in 23, seven and four cases, respectively. The 5-year progression-free rate (75%) and survival rate (83%) in 39 patients with G3 did not show a statistically significant difference from those of the 109 patients with G1 or the 187 patients with G2-superficial bladder cancer who were treated with TUR initially. Only the rate of recurrence of patients with G3 was significantly higher than that of patients with G2 or G1. Adjuvant therapies reduced the recurrence rate of the patients with G3. Only tumor morphology, papillary or non-papillary, affected both the progression-free rate and the survival rate of patients with G3. There were no statistically significant differences associated with other factors.
    Conclusion: The results suggest that superficial G3 bladder cancer could be treated with TUR initially, especially for papillary tumors.

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  • Prostate specific antigen complexed to alpha-1-antichymotrypsin in patients with intermediate prostate specific antigen levels 査読

    T Saika, T Tsushima, Y Nasu, N Kusaka, Y Miyaji, H Takamoto, K Takeda, S Uno, H Kumon

    CANCER   94 ( 6 )   1685 - 1691   2002年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JOHN WILEY & SONS INC  

    BACKGROUND. The authors attempted to evaluate prospectively the usefulness of serum prostate specific antigen (PSA) complexed to alpha-1-antichymotrypsin (PSA-ACT) in the early detection of prostate carcinoma and its ability to discriminate between prostate carcinoma and benign prostatic hyperplasia (BPH), especially among patients with intermediate PSA levels.
    METHODS. Between December 1999 and August 2000, systematic sextant biopsies were performed on 281 prospective patients with prostate carcinoma who had serum PSA levels between 4.1 ng/mL and 20.0 ng/mL. The serum samples were assayed by using kits that were designed specifically for measuring serum PSA, PSA-ACT, and free PSA levels. The clinical values of PSA, PSA-ACT, the free PSA to total PSA ratio (F/T ratio), the free PSA to PSA-ACT ratio, PSA density (PSAD), and PSA-ACT density (ACTD) were compared by using receiver operating characteristic (ROC) curve analysis.
    RESULTS. Biopsy yielded no evidence of malignancy in 198 patients, and prostate carcinoma was confirmed in 83 patients. ROC analysis demonstrated that the area under the curve (AUC) for PSA-ACT was greater than that for total PSA and was equivalent to that for the F/T ratio in both groups of patients (PSA ranges of 4.1-20.0 ng/mL and 4.1-10.0 ng/mL, respectively). The AUC for the ACTD was greater than the AUC for the PSAD and had the highest value of all parameters.
    CONCLUSIONS. The measurement of PSA-ACT represents an alternative to the use of total and free PSA. The ACTD value is the most useful for discriminating between BPH and prostate carcinoma. (C) 2002 American Cancer Society

    DOI: 10.1002/cncr.10377

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  • Validation of transrectal ultrasonographic volumetry for orthotopic prostate tumours in mice

    Kraaji R, van Weerden W.M, de Ridder C.M, Gussenhoven E.J, Honkoop J, Nasu Y, Bangma C.H

    Lab Anim   36 ( 2 )   165 - 172   2002年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Laparoscopic radical prostatectomy:Initial cases at Okayama University Hospital

    Nagai A, Nasu Y, Shirasaki Y, Iguchi H, Arata R, Tsugawa M, Tsushima T, Kumon H

    Acta Med. Okayama   56 ( 1 )   51 - 52   2002年

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    記述言語:英語   掲載種別:研究論文(大学,研究機関等紀要)  

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  • Gene therapy for prostate cancer: toxicological profile of four HSV-tk transducing adenoviral vectors regulated by different promoters 査読

    S Ebara, S Shimura, Y Nasu, H Kaku, H Kumon, G Yang, J Wang, TL Timme, E Aguilar-Cordova, TC Thompson

    PROSTATE CANCER AND PROSTATIC DISEASES   5 ( 4 )   316 - 325   2002年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Adenoviral vector delivery of the Herpes simplex virus thymidine kinase (HSV-tk) gene in combination with the prodrug ganciclovir (GCV) has been tested in phase I clinical trials for prostate cancer and found to exhibit a satisfactory toxicity profile. We have developed additional adenoviral vectors with differing promoters to optimize the expression profile and in the present study evaluate the potential systemic toxicity of these vectors. Four recombinant adenoviral vectors that express the HSV-tk gene were generated using three different promoters: CMV (leftward orientation); RSV (both rightward and leftward orientation); and the mouse caveolin-1 (cav-1) promoter (leftward orientation). Efficacy was determined in vitro by cytotoxicity assays in a mouse prostate cancer cell line, RM-9, and in vivo by treating orthotopic tumors. Potential toxicity was evaluated from liver histology and apoptotic cell counts and enzyme levels in the serum following intravenous adenoviral vector injection. Although there were differences in HSV-tk expression at the protein level among the four vectors there were no significant differences in in-vitro cytotoxicity studies with GCV or in vivo in tumor growth suppression of an orthotopic mouse prostate cancer model in GCV treated mice. Intravenous delivery of high doses of all adenoviral vectors lead to abnormalities in liver function as measured by specific serum markers and histological evaluation of liver tissue and increased levels of apoptosis in the liver. These abnormalities were most prevalent with the vector containing the CMV promoter and the rightward oriented RSV promoter. They were least prevalent in the vector regulated by the cav-1 promoter. Upregulation of specific chemokines, MIP-2 and MIP-1beta was correlated with apoptotic counts. Our results demonstrate that comprehensive toxicological analysis of adenoviral vectors provides internally consistent information that can differentiate vectors with comparable efficacy based on toxicity. In these studies vectors with the cav-1 promoter-driven and leftward RSV-driven HSV-tk gene demonstrated minimal toxicities with cytotoxic effectiveness comparable to more toxic vectors. Our studies further suggest that promoter selection can influence the toxic effects of an adenoviral gene therapy vector.

    DOI: 10.1038/sj.pcan.4500610

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  • Orthotopic neobladder reconstruction in elderly bladder cancer patients 査読

    T Saika, B Suyama, T Murata, D Manabe, T Kurashige, Y Nasu, T Tsushima, H Kumon

    INTERNATIONAL JOURNAL OF UROLOGY   8 ( 10 )   533 - 538   2001年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL SCIENCE ASIA  

    Background: We compared the clinical results of orthotopic neobladder reconstruction in elderly patients and those in younger patients retrospectively in order to verify whether age is a critical factor in selecting a method of urinary diversion.
    Methods: Following radical cystectomy for bladder cancer, 12 patients aged 75 or older and 17 patients under 75 who underwent orthotopic neobladder reconstruction between January 1992 and May 1999 were investigated in this study. The authors TS and BS were among the surgeons who performed operations for all cases. Of the 12 elderly patients, orthotopic neobladders were constructed according to Hautmann's method in nine cases, Studer's method in one case and Reddy's method in two cases. Of the 17 younger patients, these methods were employed in 12, one and four cases, respectively. Operative procedure, early and late complications, prognosis, continence and voiding pattern were investigated in these patients.
    Results: The follow-up periods for elderly and younger groups ranged from 21.3 to 82.7 months and from 8.8 to 94.2 months, respectively. No difference in operation time, amount of bleeding or postoperative length of hospitalization was observed between elderly and younger patients. The rates of early complications in elderly and younger patients were 41.7% and 35.3%, respectively. Late complication rates were 33.3% and 47.1%, respectively The difference in these complication rates was not statistically significant. One of the elderly and two of the younger patients had local recurrence and metastasis postoperatively. Those three patients had died of their bladder cancer. No statistically significant difference between groups was recognized in either cause-specific survival or overall survival, nor was there such a difference in relation to micturition/continence.
    Conclusion: Based on these results, we believe that because age is not a critical factor in the selection of urinary diversion method, neobladder reconstruction following cystectomy for bladder cancer is indicated in elderly patients. As stoma management is difficult for the patients, we consider orthotopic neobladder reconstruction to be the method of choice if the patients' general physical condition allows.

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  • Retroperitoneoscopic pyelotomy combined with the transposition of crossing vessels for ureteropelvic junction obstruction

    Nagai A, Nasu Y, Hashimoto H, Tsugawa M, Yasui K, Kumon H

    The Journal of Urology   165   23 - 26   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Optimal starting time for flutamide to prevent disease flare in prostate cancer patients treated with a gonadotropin-releasing hormone agonist 査読

    T Tsushima, Y Nasu, T Saika, Y Maki, M Noda, B Suyama, T Yamato, H Kumon

    UROLOGIA INTERNATIONALIS   66 ( 3 )   135 - 139   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    Objective: Flare-up phenomena, such as an increase in prostate-specific antigen (PSA) and/or deterioration of symptoms, are observed in some patients undergoing gonadotropin-releasing hormone (GnRH) agonist therapy. This study was carried out to determine the optimal time for starting the administration of flutamide to prevent flare-up phenomena. Patients and Methods: Twenty-six patients with prostate cancer and elevated serum levels of PSA were randomly assigned to 5 groups. Group A patients (n = 6) were treated with a subcutaneous injection of 3.75 mg leuprorelin acetate depot alone. Group B, C, D and E patients (5 patients in each group) were treated with 375 mg/day of orally administered flutamide combined with leuprorelin. Flutamide was initiated on the day of leuprorelin injection in group B, and at 1, 2 and 4 weeks before leuprorelin injection in groups C, D and E, respectively. Serum PSA and testosterone levels were measured in each patient. Results: Pretreatment with flutamide increased the serum testosterone level, but the testosterone surge after leuprorelin administration was almost the same in all 5 treatment groups. In patients who had been treated with flutamide in combination with leuprorelin, the mean PSA level did not exceed the pretreatment levels after leuprorelin administration. The rate of decrease in PSA in the group receiving simultaneous administration of flutamide with leuprorelin showed a decline comparable to that during the period before leuprorelin administration in the flutamide pretreatment groups. Conclusion: Simultaneous administration of flutamide with a GnRH agonist is sufficient to prevent flare-up phenomena. Copyright (C) 2001 S. Karger AG, Basel.

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  • Treatment of androgen-independent prostate cancer with dexamethasone: A prospective study in stage D2 patients 査読

    T Saika, N Kusaka, T Tsushima, T Yamato, T Ohashi, B Suyama, R Arata, Y Nasu, H Kumon

    INTERNATIONAL JOURNAL OF UROLOGY   8 ( 6 )   290 - 294   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL SCIENCE ASIA  

    Purpose: In order to evaluate the efficacy of dexamethasone in the treatment of Japanese men with androgen-independent prostate cancer, a prospective study was conducted using prostate-specific antigen (PSA) as a primary end-point.
    Methods: Nineteen Japanese men with stage D2 androgen-independent prostate cancer were registered and treatment was started. After ruling out anti-androgen withdrawal syndrome, they were treated with dexamethasone (1.5 mg daily). Patients were monitored for PSA, symptoms, radiologic response, survival rate, time to disease progression, time to treatment failure and complications.
    Results: Prostate-specific antigen levels decreased in nine patients (50.0%); five (27.8%) showed a 50% or greater decrease and two (11.1%) showed an 80% or greater decrease. For the nine patients, the mean duration of PSA response was 7.3 months and the median duration was 2.1 months (range, 1.2-27.5+). Bone pain, which was noted in 13 patients at study entry, improved in seven patients (53.8%). Of nine patients who had serial radiographic examinations with bone scan, three (33%) showed partial response, two (22%) were stable and four (44%) showed disease progression. Treatment was well tolerated, except for one patient who suffered a severe pulmonary infection.
    Conclusion: Dexamethasone decreased PSA levels and produced subjective symptomatic improvement in the patients with stage D2 androgen-independent prostate cancer.

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  • Noninvasive urodynamic evaluation of bladder outlet obstruction using doppler ultrasonography

    Ozawa H, Chancellor M.B, Ding Y.Y, Nasu Y, Yokoyama T, Kumon H

    Urology   56 ( 3 )   408 - 412   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Reliability of color doppler ultrasound urodynamics in the evaluation of bladder outlet obstruction

    Yew Yoong Ding, Ozawa H, Yokoyama T, Nasu Y, Chancellor M.B, Kumon H

    Urology   56 ( 6 )   967 - 971   2000年

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  • The clinical value of contrast-enhanced color doppler ultrasound : is it really useful in the diagnosis of prostate cancer?

    Nasu Y, Saika T, Arata R, Tsugawa M, Tsushima T, Kumon H, Seno T

    J Urol   163 ( 4 )   280   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Primary non-Hodgkin lymphoma of the ureter 査読

    H Hashimoto, M Tsugawa, Y Nasu, T Tsushima, H Kumon

    BJU INTERNATIONAL   83 ( 1 )   148 - 149   1999年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL SCIENCE LTD  

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  • Clinical evaluation of serum prostate-specific antigen-alpha1-antichymotrypsin complex values in diagnosis of prostate cancer:A cooperative study

    Kuriyama M, Ueno K, Uno H, Kawada Y, Akimoto S, Noda M, Nasu Y, Tsushima T, Ohmori H, Sakai H, Saito Y, Meguro N, Usami M, Kotake T, Suzuki Y, Arai Y, Shimazaki J

    Int J Urol   5 ( 1 )   48 - 54   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Gene therapy for prostate cancer

    Bangma C.H, Thompson T.C, Timme T.L, Scardino P.T, Yang G, Wang J, Nasu Y, Park S.H, Hull J.W

    Prostate Cancer and Prostatic Diseases   1 ( S-1 )   33   1998年

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    記述言語:英語  

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  • Prospective randomized comparative study of antibiotic prophylaxis in urethrocystoscopy and urethrocystography

    Tsugawa M, Monden K, Nasu Y, Kumon H, Ohmori H

    Int J Urol   5 ( 5 )   441 - 443   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Absorption of epirubicin instilled intravesically immediately after transurethral resection of superficial bladder cancer 査読

    T Tsushima, Y Miyaji, M Noda, Y Nasu, H Kumon, H Ohmori

    UROLOGIA INTERNATIONALIS   60 ( 3 )   161 - 164   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    As postoperative adjuvant therapy for superficial bladder cancer, intravesical instillation therapy is commonly conducted. In this case, from the view point of prevention of intraoperative dissemination, commencement of instillation therapy at an early postoperative period is preferred. However, increased drug permeability is suspected because of damage to the bladder mucosa during operation. Therefore, this study was conducted to investigate the plasma level of epirubicin (EPI) instilled immediately after transurethral operation. EPI (20 mg/40 ml or 50 mg/100 ml) was instilled immediately after a transurethral operation, and retained in the bladder for 1 h. Blood samples were obtained before instillation, as well as 30, 60, 120 and 240 min after instillation, and EPI levels were assayed. The mean EPI concentrations (ng/ml) among the 20-mg/40 ml group (n = 5) were <2.5 and <2.0 at 30 and 60 min, respectively, after which they were undetectable. The 50-mg/100 ml group (n = 5) recorded 5.0, 4.4 and <3.0 after 30, 60 and 120 min, respectively, and after 240 min it was undetectable. Intravesical instillation of EPI immediately after a transurethral operation causes a small increase in the plasma level and it is thought to cause small systemic side effects.

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  • INHIBITION OF A PRIMARY MOUSE PROSTATE TUMOR WITH ADENOVIRAL MEDIATED HERPES SIMPLEX VIRUS THYMIDINE KINASE GENE TRANSDUCTION & GANCICLOVIR THERAPY

    Hall S.J, Yang G, Mutchnik S.E, Adier H.L, Nasu Y, Timme T.L, Woo S.L.C, Thompson T.C

    The Journal of Urology   157 ( S-4 )   368   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • THE EFFECT OF A GONADOTROPIN-RELEASING HORMONE AGONIST ON BONE MINERAL DENSITY OF PROSTATIC CANCER PATIENTS

    Noda M, Tsushima T, Nasu Y, Ohmori H

    The Journal of Urology   157 ( S-4 )   232   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Study of the Nephrotoxicity of Iron Oxide Fumes Released by Welding in an Experimental Model

    Noda M, Tsushima T, Nasu Y, Kumon H, Ohmori H, Okada S

    Pathophysiology   4   221 - 226   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Clinical Studies on Advantages and Safety of Visual Laser Ablation for Patients with Benign Prostatic Hyperplasia

    Nasu Y, Watanabe T, Kumon H, Ohmori H

    Int J Urol   3 ( S-1 )   S53 - S54   1996年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • New Technological Methods of Managing BPH -Visual Laser Ablation of Prostate(VLAP):Non-contact-

    Nasu Y, Kumon H, Ohmori H

    Japanese Journal of Endourology and ESWL   9 ( 2 )   137 - 139   1996年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Relationship of intracellular concentration and duration of contamination of pirarubicin and adriamycin in human bladder cancer cell lines and human bladder normal mucosa cell line 査読

    T. Saika, T. Tsushima, Y. Nasu, N. Akebi, H. Ohmori

    Japanese Journal of Cancer and Chemotherapy   23 ( 7 )   905 - 909   1996年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    To establish a method for reasonable clinical use of adriamycin (ADM) and pirarubicin (THP) in the intravesical chemotherapy for superficial bladder cancer, intracellular concentrations of these drugs were examined in culture cell lines (T-24, T-24/ADM and FHS736b1) with variable durations of contamination. The intracellular concentration of THP showed a plateau at 15- 30 min. contamination in T-24, and in T-24/ADM, and showed the time dependence of contamination in FHS736b1, human normal bladder mucosa cell line. The intracellular concentration of ADM showed the time dependence of contamination in T-24, T-24/ADM and FHS736b1. And these concentrations of THP were 20 times higher than those of ADM. In conclusion, it seems better that THP was retained for 5-15 min. in the bladder in the intravesical chemotherapy, from the point of view of drug efficacy and preventing side effects. And it seems good that ADM was retained for more than 30 min. in the case with drug sensitive tumors.

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  • EPIDERMAL GROWTH FACTOR AS A MARKER FOR RECURRENCE OF THE BLADDER CANCER 査読

    T SAIKA, T TSUSHIMA, Y NASU, M NODA, Y MORIYA, H OHMORI

    PROCEEDINGS OF THE XVI INTERNATIONAL CANCER CONGRESS - FREE PAPERS AND POSTERS, TOMES 1-4   2145 - 2149   1994年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:MONDUZZI EDITORE  

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  • THERAPEUTIC OUTCOME OF G-CSF THERAPY ON ADVANCED TESTICULAR TUMORS 査読

    T TSUSHIMA, M TAKAMATSU, T SAIKA, Y MAKI, N AKEBI, Y NASU, H OHMORI

    PROCEEDINGS OF THE XVI INTERNATIONAL CANCER CONGRESS - FREE PAPERS AND POSTERS, TOMES 1-4   2225 - 2229   1994年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:MONDUZZI EDITORE  

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  • OVER‐EXPRESSION OF METALLOTHIONEIN AND DRUG‐RESISTANCE IN BLADDER CANCER 査読

    Takashi Saika, Tomoyasu Tsushima, Jyunzo Och, Naoki Akebi, Yasutomo Nasu, Yosuke Matsumura, Hiroyuki Ohmori

    International Journal of Urology   1 ( 2 )   135 - 139   1994年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Metallothionein (MT) in tumor cells has been implicated as one of the factors involved in mechanisms of resistance to anti‐cancer drugs, including cis‐diaminedichroloplatinum (CDDP) and adriamycin (ADM). The relationship between the expression of MT and chemotherapy with anti‐cancer drugs was studied in CDDP‐ and ADM‐resistant human bladder cancer cell lines and tissue samples from clinical cases. In drug‐resistant cell lines (T‐24/ADM, CI‐7/CDDP) established in our laboratory, MT expression was studied by immunohistochemistry using the avidin‐biotin peroxidase complex (ABC) method and radioimmunoassay (RIA), using anti‐MT antibody. In addition, other potentfal mechanisms of drug resistance, such as P‐glycoprotein expression were examined and the levels of reduced glutathione (GSH), oxidized glutathione (GSSG) and glutathione‐S‐transferase (GST) determined in these cell lines. The results of these investigations demonstrate that the expression of MT in resistant cell lines increased 2.1‐ and 2.5‐fold when compared with parent cell lines (CI‐7, T‐24). GSH, GSSG and GST levels were unchanged and P‐glycoprotein was not over‐expressed. A total of 120 tissue samples from 35 clinical cases of bladder cancer, before and after chemotherapy, were stained for MT which was detected in 10 of the 35 cases before chemotherapy. The incidence of MT expression was significantly higher (p less than 0.05) in cases with lower pathological tumor grades. By analyzing the MT staining after chemotherapy in the cases whose MT staining was negative before chemotherapy, it was found that cases receiving continuous administration (intravesical chemotherapy or peroral chemotherapy) showed a higher incidence (9/13) of positive staining for MT, than patients receiving intermittent administration (intravenous chemotherapy) (1/8), (p less than 0.05). These results demonstrate that: 1) a correlation exists between MT expression and tumor differentiation and 2) repetitive and continuous administration of anti‐cancer drugs results in increased MT expression in bladder cancer cells. MT expression may therefore be one of the mechanisms by which urothelial tumors acquire resistance to anti‐cancer drugs. Copyright © 1994, Wiley Blackwell. All rights reserved

    DOI: 10.1111/j.1442-2042.1994.tb00022.x

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  • Clinical study of urethral recurrence of superficial bladder cancer : its clinical significance as a prognostic implication

    Nasu Y, Tsushima T, Akebi N, Maki Y, Takamatsu M, Inoue T, Ohmori H

    J Urol   151 ( 5 )   232A   1994年

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  • Hyperthermotherapy Added to the Multidisciplinary Therpay for Penile Cancer

    Kuroda M, Tsushima T, Nasu Y, Asaumi J, Nishikawa K, Gao X.S, Joja I, Takeda Y, Togami I, Makihata E, Kawasaki S, Ohmor H, Hiraki Y

    Acta Med. Okayama   47 ( 3 )   169 - 174   1993年

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    記述言語:英語   掲載種別:研究論文(大学,研究機関等紀要)  

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  • Endopyeloplasty for Ureteropelvic Junction Obstruction with a New, Multifunction Surgical Endoscope in Children

    Kumon H, Nasu Y, Ono N, Hayashi T, Ohmori H

    Japanese Journal of Endourology and ESWL   6 ( 1 )   35 - 38   1993年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Tissue concentration of intravesically instilled (2″R)-4′-0-tetrahydropyranyl-adriamycin or adriamycin in superficial bladder cancer 査読

    Takashi Saika, Tomoyasu Tsushima, Yasutomo Nasu, Masatoshi Noda, Naoki Akebi, Syunko Kaku, Masatake Takamatsu, Hiroyuki Ohmori, Satoru Uno, Taiichiro Johsen

    Japanese Journal of Urology   84 ( 7 )   1206 - 1210   1993年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Twenty-one patients with superficial bladder cancer entered an analysis of single dose (2′R)-4′-0-tetrahydropyranyladriamycin (THP) or adriamycin (ADM) administration. The patients in each group that have been or not have been treated previously with anti-cancer drugs were randomized into two groups, one was given THP and the other ADM. Thirty-mg of THP or ADM dissolved in 30 ml of physiologic saline was instilled into the bladder, and retained for 1 hour. After 1 hour retention of the drugs, tumor tissues and normal mucosas were removed by punch biopsy forceps transurethrally. The tissue concentrations of THP and ADM were estimated by high performance liquid chromatography. The tissue concentrations of THP and ADM in the tumors were significantly greater (p&lt
    0.05) than those in the normal bladder mucosas. The tissue concentration of THP in the tumors were greater than that of ADM. The tissue concentrations of THP and ADM in the tumor of patients who have been treated with anti-cancer drugs priviously were less than those of patients who have not. This results demonstrated that prior therapy with anti-cancer drug may cause a resistance for intravesical instillation chemotherapy. However in patients with prior therapy, the tissue concentration of THP in the tumors were greater than that of ADM. Based on these findings, THP has been shown to be were effective as an intravesical instillating agent expecially, in cases with prior chemotherapy. © 1993, THE JAPANESE UROLOGICAL ASSOCIATION. All rights reserved.

    DOI: 10.5980/jpnjurol1989.84.1206

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  • Phase 2 study of 5'-DFUR for bladder and prostatic cancer 査読

    H. Ohmori, Y. Matsumura, J. Ochi, K. Kobashi, N. Akebi, T. Saika, K. Nanba, T. Tanahashi, T. Josen, Y. Nasu, M. Saegusa, Y. Ozaki, M. Hara, T. Asahi, N. Ike, T. Tsushima, N. Mitsuhata, T. Akagi, T. Kaeda

    Japanese Journal of Cancer and Chemotherapy   18 ( 13 )   2307 - 2314   1991年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    Phase 2 study of 5'-DFUR in bladder and prostatic cancer was conducted at 15 collaborative institutions including Okayama University. 5'-DFUR was orally administered to patients at a daily dose of 800-1200 mg for more than 4 weeks. Forty-one patients with bladder cancer and 12 patients with prostatic cancer were evaluated. The response rate for bladder cancer was 31.7% (CFR, 1 case: PR, 12 cases), against no response with prostatic cancer. Moreover, the concentration of 5-FU in bladder tumors was confirmed to be high. Adverse reactions such as diarrhea, anorexia, and nausea were observed. Thus, 5'-DFUR seems to be useful for the treatment of bladder cancer.

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  • A COMBINED LIGHT AND SCANNING ELECTRON-MICROSCOPY ON HUMAN URINARY CYTOLOGY 査読

    H KUMON, Y NASU, Y MATSUMURA, H OHMORI, T TANAKA

    JOURNAL OF ELECTRON MICROSCOPY   32 ( 1 )   27 - 32   1983年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

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書籍等出版物

  • 5.医学・薬学・看護学 5.4先進医療 5.4.2遺伝子医療(治療)

    光井洋介, 那須保友, 一般社団法人日本医療情報学会医療情報技師育成部会, 代表, 岡田美保子( 担当: 分担執筆 ,  範囲: 医療情報 第6版:医学医療編)

    篠原出版新社,東京  2019年3月 

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  • Robotic-assisted renal autotransplantation:Preliminary studies and future directions

    Araki M, Wada K, Kawamura K, Maruyama Y, Mitsui Y, Sadahira T, Kubota R, Nishimura S, Yoshioka T, Ariyoshi Y, Fujio K, Takamoto A, Sugimoto M, Sasaki K, Kobayashi Y, Ebara S, Amano H, Inui M, Watanabe M, Watanabe T, Nasu Y, Editors.Chan S.Y, Matsuda T( 担当: 分担執筆 ,  範囲: Endourology Progress)

    Springer,Bundesrepublik Deutschland  2019年 

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  • 12.男性の排尿障害~老化現象とあきらめないで

    那須保友( 担当: 分担執筆 ,  範囲: 女と男のディクショナリーHUMAN+)

    公益遮断法人日本産科婦人科学会,横浜市  2018年9月 

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  • Ⅴ薬物療法(去勢抵抗性前立腺癌):61 去勢抵抗性前立腺癌に対する薬剤の使用手順の決定に重要な因子は何ですか?

    谷本竜太, 那須保友( 担当: 分担執筆 ,  範囲: Q&Aでスッキリわかる前立腺癌)

    株式会社メジカルビュー社,東京  2017年8月 

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  • 16-43 持続勃起症

    那須保友, 監修, 猿田享男, 喜多村惣一郎( 担当: 分担執筆 ,  範囲: 1336専門家による私の治療 2017-18年度版)

    日本医事新報社,東京  2017年7月 

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  • 第4章がんと遺伝子治療 1.臨床の現場に近づいた前立腺がん遺伝子治療の現状と今後の展開

    那須保友( 担当: 分担執筆 ,  範囲: トランスレーショナルリサーチを支援する遺伝子医学MOOK30 今、着実に実り始めた遺伝子治療ー最新研究と今後の展開)

    メディカル ドゥ,大阪  2016年6月 

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  • 5.4.2遺伝子医療(治療)

    那須保友, 一般社団法人日本医療情報学会医療情報技師育成部会( 担当: 分担執筆 ,  範囲: 医療情報第5版 医学・医療編)

    篠原出版新社,東京  2016年3月 

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  • 膀胱尿管逆流症

    那須保友, 福井次矢, 高木 誠, 小室一誠( 担当: 分担執筆 ,  範囲: 今日の治療指針 私はこう治療している)

    医学書院,東京  2014年1月 

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  • 泌尿器科疾患 Wilms腫瘍

    那須保友, 山口, 徹, 北原光夫, 福井次矢( 担当: 分担執筆 ,  範囲: 今日の治療指針 私はこう治療している)

    医学書院,東京  2011年1月 

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  • 遺伝子治療の最先端

    賀来春紀, 那須保友, 公文裕巳, 村井, 勝, 奥山明彦, 内藤誠二編( 担当: 分担執筆 ,  範囲: 泌尿器疾患治療の新しいストラテジー)

    メジカルビュー社,東京  2007年 

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  • 中皮腫に対する遺伝子治療

    柏倉祐司, 那須保友, 公文裕巳( 担当: 分担執筆 ,  範囲: 胸膜中皮腫診療ハンドブック)

    中外医学社,東京  2007年 

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  • Chapter 10 Committee 13 New therapeutic targets and treatments for metastatic prostate cancer:New therapeutic targets and treatments for metastatic prostate cancer

    Nelson W.G, Saad F, Debruyne F.M.J, Eisenberger M.A, Fourcade R, Frenkel E.P, Kantoff P.W, Kumon H, Nasu Y, Oudard S, Wirth M.(editors. Mcconnell J, Denis L, Akaza H, Khoury S, Schalken J( 担当: 分担執筆 ,  範囲: Prostate Cancer)

    Health Publications,Paris  2006年 

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  • Indications for ureteroscopy

    Kumon H, Abarzua F, Nasu Y, Smith A.D, Badlani G.H, Bagley D.H, Clayman R.V, Docimo S.G, Jordan G.H, Kavoussi L.R, Lee B.R, Lingeman J.E, Preminger G.M, Segura J( 担当: 分担執筆 ,  範囲: Smith's Textbook of Endourology Second Edition)

    BC Decker Inc,London  2006年 

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  • Targeting energy-assisted gene delivery in urooncology

    Nasu Y, Abarzua F, Kumon H, Kumon H, Murai M, Baba S. E( 担当: 分担執筆 ,  範囲: Recent Advances in Endourology 6 Endourooncology New Horizons in Endourology)

    Springer  2005年 

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  • 泌尿器科領域 3.前立腺癌の遺伝子治療

    那須保友, 伊藤克己, 浅野 泰, 遠藤 仁, 御手洗哲也, 東原英二( 担当: 分担執筆 ,  範囲: Annual Review 腎臓)

    中外医学社,東京  2003年 

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  • 腎機能検査

    那須保友, 日下信行( 担当: 分担執筆 ,  範囲: 看護のための最新医学講座22泌尿・生殖器疾患)

    中山書店,東京  2001年 

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  • 泌尿器科癌に対する遺伝子治療

    那須保友, 勝岡洋治, 赤座英之( 担当: 分担執筆 ,  範囲: 泌尿器科悪性腫瘍治療ハンドブック)

    新興医学出版社,東京  2001年 

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  • Bone metastasis of urogenital cancer

    Bone metastasis and related disease  1995年 

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  • IV骨転移診療の実際 5章 泌尿器科領域悪性腫瘍の骨転移

    骨転移 病態・診断・治療 金芳堂  1995年 

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MISC

  • 腎門部に発生した粘液囊胞腺癌の 1 例

    三宅修司, 岩田健宏, 長尾賢太郎, 河田達志, 富永悠介, 定平卓也, 西村慎吾, 高本 篤, 佐古智子, 和田耕一郎, 枝村康平, 小林康之, 荒木元朗, 渡部昌実, 渡辺豊彦, 柳井広之, 那須保友

    西日本泌尿器科   83 ( 2 )   100 - 105   2021年6月

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    記述言語:日本語  

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  • 腎癌-最新の診断-局所療法(凍結療法,ラジオ波焼却術を含む)

    高本 篤, 荒木元朗, 那須保友

    日本臨牀   79 ( 5 )   753 - 758   2021年5月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • 遠隔治療 泌尿器科編

    富永悠介, 荒木元朗, 那須保友

    岡山医学会雑誌   133 ( 1 )   73 - 75   2021年4月

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    記述言語:日本語  

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  • 第34回日本泌尿器内視鏡学会総会開催報告

    那須保友

    岡山医学会雑誌   133 ( 1 )   83 - 84   2021年4月

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    記述言語:日本語  

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  • 若年発症の前立腺炎症性偽腫瘍の 1 例

    佐久間貴文, 丸山雄樹, 定平卓也, 高本 篤, 和田耕一郎, 小林泰之, 荒木元朗, 渡部昌実, 渡辺豊彦, 那須保友

    西日本泌尿器科   83 ( 1 )   54 - 58   2021年4月

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    記述言語:日本語  

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  • 遺伝性平滑筋腫症-腎細胞癌症候群関連腎細胞癌の術後骨転移に対してイピリムマブ・ニボルマブ併用療法が有効であった1例

    渡部智文, 榮枝一磨, 津川昌也, 関戸崇了, 富永悠介, 高本 篤, 定平卓也, 小林泰之, 荒木元朗, 渡辺豊彦, 小田和歌子, 黒田直人, 十川麗美, 山本英喜, 平沢 晃, 那須保友

    西日本泌尿器科   83 ( 1 )   31 - 36   2021年4月

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    記述言語:日本語  

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  • 抗 TFE3 抗体による免疫染色陽性所見からXp11.2 転座型腎癌と考えられた 3 例

    井上陽介, 関戸崇了, 定平卓也, 高本 篤, 杉本盛人, 和田耕一郎, 荒木元朗, 那須保友

    西日本泌尿器科   83 ( 1 )   42 - 47   2021年4月

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    記述言語:日本語  

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  • 第323回日本泌尿器科学会岡山地方会演題抄録 岡山大学病院における去勢抵抗性前立腺癌に対するドセタキセルの長期治療成績

    河田達志, 高本 篤, 横山周平, 宗田大二郎, 坪井一馬, 長尾賢太郎, 富永悠介, 岩田健宏, 西村慎吾, 佐古智子, 枝村康平, 小林泰之, 荒木元朗, 渡辺豊彦, 那須保友

    西日本泌尿器科   82 ( 6 )   605   2021年2月

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    記述言語:日本語  

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  • 神経線維腫症1型に合併した後腹膜悪性末梢神経鞘腫瘍の1例

    宗田大二郎, 岩田健宏, 定平卓也, 富永悠介, 片山 聡, 西村慎吾, 高本 篤, 佐古智子, 枝村康平, 小林泰之, 荒木元朗, 渡部昌実, 渡辺豊彦, 那須保友, 中田英二, 柳井広之

    西日本泌尿器科   82 ( 6 )   596 - 600   2021年

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    記述言語:日本語  

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  • 排尿障害を伴う全周性尿道憩室に対して外科的治療を行った1例

    松尾聡子, 谷本竜太, 杉本盛人, 津島知靖, 中村あや, 定平卓也, 和田里章悟, 小林泰之, 荒木元朗, 渡辺豊彦, 那須保友

    西日本泌尿器科   82 ( 5 )   514 - 517   2020年12月

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    記述言語:日本語  

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  • Lynch症候群に発症した上部尿路上皮癌の1例

    本郷智拡, 神原太樹, 野田 岳, 森 聰博, 中田哲也, 田中屋宏爾, 赤木 究, 定平卓也, 荒木元朗, 那須保友

    西日本泌尿器科   82 ( 4 )   477 - 482   2020年10月

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    記述言語:日本語  

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  • ニボルマブ・イピリムマブ併用療法による血球貧食症候群を発症した1例

    笹岡丈人, 平田武志, 倉繁拓志, 早田俊司, 富永悠介, 光井洋介, 定平卓也, 高本 篤, 小林泰之, 荒木元朗, 渡辺豊彦, 那須保友

    西日本泌尿器科   82 ( 4 )   449 - 452   2020年10月

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    記述言語:日本語  

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  • 腎摘除術を要した多房性気腫性腎盂腎炎の1例

    小林宏州, 高本 篤, 定平卓也, 和田耕一郎, 前原貴典, 大岩裕子, 岩田健宏, 西村慎吾, 杉本盛人, 小林泰之, 荒木元朗, 渡部昌実, 渡辺豊彦, 那須保友

    西日本泌尿器科   82 ( 4 )   465 - 469   2020年10月

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    記述言語:日本語  

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  • ロボット手術の教育方法-それぞれの施設での試み-岡山大学病院泌尿器科におけるロボッ手術教育の現状

    小林泰之, 岩田健宏, 西村慎吾, 高本 篤, 佐古智子, 定平卓也, 枝村康平, 荒木元朗, 渡辺豊彦, 那須保友

    Japanese Journal of Endourology   33 ( 2 )   221 - 224   2020年9月

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    記述言語:日本語  

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  • 特集 人工知能(AI)を活用した医療の展開 序説 人工知能(AI)への期待:Development of artificial intelligence (AI) in medical field

    那須保友

    岡山医学会雑誌   132   91   2020年8月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(大学・研究所紀要)  

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  • 【腎盂・尿管癌の治療を考える】腎盂・尿管癌における腹腔鏡下腎尿管全摘除術の適応

    吉永香澄, 荒木元朗, 和田里章悟, 丸山雄樹, 光井洋介, 窪田理沙, 定平卓也, 岩田健宏, 西村慎吾, 高本 篤, 和田耕一郎, 佐古智子, 枝村康平, 小林泰之, 渡部昌実, 渡辺豊彦, 那須保友

    泌尿器外科   33 ( 5 )   455 - 461   2020年5月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • 岡山大学病院における進行性尿路上皮癌に対するpembrolizumabの治療経験

    富永悠介, 高本 篤, 関戸崇了, 和田里章悟, 大岩裕子, 窪田理沙, 片山 聡, 岩田健宏, 西村慎吾, 佐古智子, 和田耕一郎, 枝村康平, 小林泰之, 荒木元朗, 渡辺豊彦, 那須保友

    西日本泌尿器科   82 ( 1 )   106 - 110   2020年4月

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    記述言語:日本語  

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  • 第321回日本泌尿器科学会岡山地方会演題抄録 後腹膜悪性末梢神経鞘腫の1例

    宗田大二郎, 高本 篤, 荒木元朗, 角南亮輔, 関戸崇了, 佐久間貴文, 和田里章悟, 吉永香澄, 丸山雄樹, 光井洋介, 富永悠介, 大岩裕子, 窪田理沙, 定平卓也, 片山 聰, 岩田健宏, 西村慎吾, 佐古智子, 和田耕一郎, 枝村康平, 小林泰之, 石井亜矢乃, 渡部昌実, 渡辺豊彦, 那須保友

    西日本泌尿器科   82 ( 1 )   173   2020年4月

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    記述言語:日本語  

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  • 学会好事:第7回泌尿器画像診断・治療技術研究会(JSURT)

    那須保友

    Urology Today   27 ( 1 )   41   2020年3月

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    記述言語:日本語  

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  • 非淋菌性尿道炎に対する 1 日 200 mg sitafloxacin 投与法

    和田耕一郎, 定平卓也, 荒木元朗, 渡辺豊彦, 那須保友

    日本化学療法学会雑誌   68 ( 2 )   181 - 185   2020年3月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • 第65回日本化学療法学会総会シンポジウム12:性感染症の治療におけるUp to date 非淋菌性尿道炎に対するSTFX 200 mg/day

    和田耕一郎, 定平卓也, 荒木元朗, 渡辺豊彦, 那須保友

    日化療会誌   68 ( 2 )   181 - 185   2020年

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    記述言語:日本語  

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  • 岡山大学病院における進行性尿路上皮癌に対するpembrolizumabの治療経験

    富永 悠介, 高本 篤, 角南 亮輔, 関戸 崇了, 和田里 章悟, 河村 香澄, 丸山 雄樹, 光井 洋介, 大岩 裕子, 窪田 理沙, 定平 卓也, 片山 聡, 岩田 健宏, 西村 慎吾, 佐古 智子, 和田 耕一郎, 枝村 康平, 小林 泰之, 荒木 元朗, 石井 亜矢乃, 渡部 昌実, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   81 ( 6 )   656 - 656   2019年12月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • Fumarate hydratase(FH)欠損腎細胞癌によるリンパ節転移に対して、イピリムマブ+ニボルマブ併用療法が奏功した一例

    関戸 崇了, 高本 篤, 小林 泰之, 三井 將雄, 和田里 章吾, 窪田 理沙, 岩田 健宏, 枝村 康平, 佐古 智子, 荒木 元朗, 渡邉 豊彦, 柳井 広之, 那須 保友

    西日本泌尿器科   81 ( 増刊 )   151 - 151   2019年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • セカンドルック前立腺生検標本を含めた病理標本の一致率の検討

    定平 卓也, 丸山 雄樹, 光井 洋介, 荒木 元朗, 小林 泰之, 渡辺 豊彦, 那須 保友

    西日本泌尿器科   81 ( 増刊 )   199 - 199   2019年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 糖尿病患者は腎部分切除術後一年で腎機能が低下する Propensity Score-Matched Analysis

    河村 香澄, 荒木 元朗, 丸山 雄樹, 光井 洋介, 定平 卓也, 佐古 智子, 和田 耕一郎, 枝村 康平, 小林 泰之, 渡辺 豊彦, 那須 保友

    西日本泌尿器科   81 ( 増刊 )   177 - 177   2019年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • S100A11は筋浸潤性膀胱癌細胞と線維芽細胞のクロストークに関与し腫瘍進行に寄与する(S100A11 contributes to tumor progression with cross talking between muscle invasive bladder cancer cells and fibroblasts)

    光井 洋介, 定平 卓也, 渡部 昌実, 丸山 雄樹, 荒木 元朗, 渡邉 豊彦, 阪口 政清, 那須 保友

    西日本泌尿器科   81 ( 増刊 )   138 - 138   2019年10月

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    記述言語:英語   出版者・発行元:西日本泌尿器科学会  

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  • 症例から学ぶ 治療変遷から読み解く腎癌治療 何を、どこまで、どのように? 下大静脈腫瘍塞栓を伴う腎腫瘍の新しい手術アプローチ

    荒木 元朗, 小林 泰之, 枝村 康平, 角南 亮輔, 関戸 崇了, 和田里 章悟, 河村 香澄, 丸山 雄樹, 光井 洋介, 大岩 裕子, 窪田 理沙, 定平 卓也, 片山 聡, 岩田 健宏, 西村 慎吾, 高本 篤, 佐古 智子, 和田 耕一郎, 渡辺 豊彦, 渡部 昌実, 那須 保友, 楳田 佑三, 八木 孝仁

    西日本泌尿器科   81 ( 増刊 )   113 - 113   2019年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 岡山大学病院における進行性尿路上皮腫瘍に対するpembrolizumabの治療経験

    高本 篤, 小林 泰之, 大岩 裕子, 片山 聡, 枝村 康平, 窪田 理沙, 岩田 健宏, 西村 慎吾, 佐古 智子, 和田 耕一郎, 荒木 元朗, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   81 ( 増刊 )   183 - 183   2019年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • サルコペニアが前立腺全摘除後の排尿関連QoLに与える影響 Expanded Prostate Cancer Index Compositeを用いた経時的変化

    光井 洋介, 渡邉 豊彦, 定平 卓也, 丸山 雄樹, 和田 耕一郎, 枝村 康平, 小林 泰之, 荒木 元朗, 渡部 昌実, 那須 保友

    日本排尿機能学会誌   30 ( 1 )   255 - 255   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本排尿機能学会  

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  • ロボット補助腹腔鏡下前立腺全摘術後尿失禁の予測因子としての骨盤MRIパラメーターの有用性

    定平 卓也, 河村 香澄, 丸山 雄樹, 光井 洋介, 和田 耕一郎, 小林 泰之, 荒木 元朗, 渡部 昌実, 渡邉 豊彦, 那須 保友

    日本排尿機能学会誌   30 ( 1 )   321 - 321   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本排尿機能学会  

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  • 生体腎移植8年後に回腸末端に発生したPTLDの一例

    和田里 章悟, 荒木 元朗, 河村 香澄, 丸山 雄樹, 光井 洋介, 窪田 理沙, 定平 卓也, 西村 慎吾, 和田 耕一郎, 枝村 康平, 小林 泰之, 渡邉 豊彦, 北川 正史, 田邊 克幸, 那須 保友

    移植   54 ( 総会臨時 )   278 - 278   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 前立腺癌に対するロボット支援前立腺全摘除術における前方・後方アプローチ間での術後QOLの比較

    丸山 雄樹, 定平 卓也, 荒木 元朗, 河村 香澄, 光井 洋介, 和田 耕一郎, 谷本 竜太, 小林 泰之, 渡部 昌実, 渡邊 豊彦, 那須 保友

    日本排尿機能学会誌   30 ( 1 )   255 - 255   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本排尿機能学会  

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  • 腎虚血再灌流障害における好中球動態の2光子励起顕微鏡を用いた解析

    丸山 雄樹, 荒木 元朗, 城所 研吾, 和田 耕一郎, 河村 香澄, 光井 洋介, 定平 卓也, 窪田 理沙, 西村 慎吾, 渡部 昌実, 渡邉 豊彦, 田邊 克幸, 和田 淳, 柏原 直樹, 那須 保友

    移植   54 ( 総会臨時 )   241 - 241   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 岡山大学病院における腹腔鏡下と後腹膜鏡下ドナー腎採取術の比較検討

    西村 慎吾, 荒木 元朗, 小林 泰之, 山下 里美, 坪井 一朗, 河村 香澄, 和田里 章悟, 丸山 雄樹, 光井 洋介, 窪田 理沙, 和田 耕一郎, 渡辺 豊彦, 那須 保友

    移植   54 ( 総会臨時 )   201 - 201   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 生体腎移植におけるドナー・レシピエントのQoL変化の相違の検討

    光井 洋介, 荒木 元朗, 山下 里美, 丸山 雄樹, 河村 香澄, 窪田 理沙, 西村 慎吾, 和田 耕一郎, 小林 泰之, 渡邉 豊彦, 北川 正史, 田邊 克幸, 杉山 斉, 和田 淳, 那須 保友

    移植   54 ( 総会臨時 )   253 - 253   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 腎移植後に発生し治療により完治したリンパ嚢腫の2例

    窪田 理沙, 荒木 元朗, 和田 耕一郎, 河村 香澄, 丸山 雄樹, 光井 洋介, 西村 慎吾, 山下 里美, 小林 泰之, 渡邉 豊彦, 那須 保友, 加納 弓月, 北川 正史, 田邊 克幸, 和田 淳

    移植   54 ( 総会臨時 )   278 - 278   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 生体腎移植8年後に回腸末端に発生したPTLDの一例

    和田里 章悟, 荒木 元朗, 河村 香澄, 丸山 雄樹, 光井 洋介, 窪田 理沙, 定平 卓也, 西村 慎吾, 和田 耕一郎, 枝村 康平, 小林 泰之, 渡邉 豊彦, 北川 正史, 田邊 克幸, 那須 保友

    移植   54 ( 総会臨時 )   278 - 278   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 生体腎移植におけるドナー・レシピエントのQoL変化の相違の検討

    光井 洋介, 荒木 元朗, 山下 里美, 丸山 雄樹, 河村 香澄, 窪田 理沙, 西村 慎吾, 和田 耕一郎, 小林 泰之, 渡邉 豊彦, 北川 正史, 田邊 克幸, 杉山 斉, 和田 淳, 那須 保友

    移植   54 ( 総会臨時 )   253 - 253   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 腎虚血再灌流障害における好中球動態の2光子励起顕微鏡を用いた解析

    丸山 雄樹, 荒木 元朗, 城所 研吾, 和田 耕一郎, 河村 香澄, 光井 洋介, 定平 卓也, 窪田 理沙, 西村 慎吾, 渡部 昌実, 渡邉 豊彦, 田邊 克幸, 和田 淳, 柏原 直樹, 那須 保友

    移植   54 ( 総会臨時 )   241 - 241   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • マージナルドナーからの生体腎移植の検討

    河村 香澄, 荒木 元朗, 関戸 崇了, 丸山 雄樹, 光井 洋介, 窪田 理沙, 西村 慎吾, 和田 耕一郎, 山下 里美, 小林 泰之, 渡邉 豊彦, 那須 保友

    移植   54 ( 総会臨時 )   324 - 324   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • マージナルドナーからの生体腎移植の検討

    河村 香澄, 荒木 元朗, 関戸 崇了, 丸山 雄樹, 光井 洋介, 窪田 理沙, 西村 慎吾, 和田 耕一郎, 山下 里美, 小林 泰之, 渡邉 豊彦, 那須 保友

    移植   54 ( 総会臨時 )   324 - 324   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 岡山大学病院泌尿器科における腹腔鏡教育プログラムの有用性 腹腔鏡下副腎摘除術

    和田里 章悟, 小林 泰之, 定平 卓也, 岩田 健宏, 西村 慎吾, 高本 篤, 佐古 智子, 枝村 康平, 和田 耕一郎, 荒木 元朗, 渡邉 豊彦, 那須 保友

    Japanese Journal of Endourology   32 ( 2 )   225 - 229   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器内視鏡学会  

    当院泌尿器科では2006年から独自の腹腔鏡手術教育プログラムを作成し手術教育を行っている。2009年3月から2017年11月までに当院で腹腔鏡技術認定医トレーニング中の泌尿器科医師が執刀した腹腔鏡下副腎摘除術76症例と、当院の腹腔鏡技術認定医が行った43症例との周術期成績の比較検討を行った。プログラム対象者は合計18人(卒後6-11年目)で、1人あたりの執刀症例数は中央値4例(range:1-9)、認定医の術者は9名で中央値2例(range:1-26)であった。患者背景には大きな差は認めなかった。手術時間は認定医群で有意に短かった(p=0.0122)ものの、両群において輸血や開腹移行症例、重篤な合併症はなかった。手術時間・出血量に急激なラーニングカーブは認めなかった。系統的な手術教育により腹腔鏡手術経験の未熟な術者の初期症例においても、安定した周術期成績を残すことが出来た。(著者抄録)

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  • 岡山大学病院における腹腔鏡下と後腹膜鏡下ドナー腎採取術の比較検討

    西村 慎吾, 荒木 元朗, 小林 泰之, 山下 里美, 坪井 一朗, 河村 香澄, 和田里 章悟, 丸山 雄樹, 光井 洋介, 窪田 理沙, 和田 耕一郎, 渡辺 豊彦, 那須 保友

    移植   54 ( 総会臨時 )   201 - 201   2019年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • ロボット支援腹腔鏡下腎部分切除術後の腎癌尿管再発の1例

    角南 亮輔, 大岩 裕子, 高本 篤, 関戸 崇了, 佐久間 貴文, 和田里 章悟, 河村 香澄, 丸山 雄樹, 光井 洋介, 窪田 理沙, 定平 卓也, 片山 聡, 岩田 健宏, 西村 慎吾, 佐古 智子, 和田 耕一郎, 枝村 康平, 小林 泰之, 荒木 元朗, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   81 ( 4 )   482 - 482   2019年8月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 消化器癌による転移性尿管腫瘍の2例

    河村 香澄, 荒木 元朗, 定平 卓也, 丸山 雄樹, 光井 洋介, 西村 慎吾, 高本 篤, 和田 耕一郎, 谷本 竜太, 杉本 盛人, 小林 泰之, 渡部 昌実, 渡辺 豊彦, 那須 保友, 柳井 広之

    西日本泌尿器科   81 ( 4 )   461 - 466   2019年8月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    消化器癌を原発とする転移性尿管腫瘍の2例を経験したため報告する。【症例1】64歳、男性。2007年9月、下行結腸癌に対して下行結腸切除術を施行した。2014年、術後のCTで右尿管腫瘍を指摘され当科へ紹介となった。右尿管鏡検査時の生検病理結果より下行結腸癌の尿管転移と診断し、開腹腫瘍摘出術を行った。術後4ヵ月の時点で肝転移を認めたためTS-1 120mg内服療法を14ヵ月行い、様々な変更を経て現在はXELIRI + Bevacizumab療法8コース目を行っている。肝転移は緩徐に増大中である。【症例2】62歳、男性。2014年12月、多発胃癌に対して幽門側胃切除術、胆嚢摘出術およびRoux-en-Y再建術を施行し、術後TS-1 100mg内服療法を1年間行った。2017年、CTで右水腎症と尿管壁肥厚を指摘され、右尿管鏡検査を行うも狭窄が強く組織は採取できず、検査時の分腎尿細胞診はclass IVであった。再施行した右尿管鏡検査において狭窄尾側を生検したところ浸潤性尿路上皮癌の診断であったため、後腹膜鏡下右腎尿管摘除術を施行した。右尿管は腹膜およびS状結腸と著しく癒着し、漿膜ごと摘除した。病理結果は胃癌の右尿管転移であった。術後weekly PTX 100mgを開始したが骨転移の出現や腹膜転移の進行を認め、PTX + Ramucirumab 1コース施行した後に抗PD-1抗体180mgを5コース行ったが全身状態は徐々に悪化した。術後7ヵ月で緩和療法に移行し、術後11ヵ月で永眠された。転移性尿管腫瘍は術前生検しても診断が難しい場合がある。予後不良な疾患であり、悪性疾患の既往のある尿管腫瘍は転移性尿管腫瘍も鑑別に挙げる必要がある。(著者抄録)

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  • 消化器癌による転移性尿管腫瘍の2例

    河村 香澄, 荒木 元朗, 定平 卓也, 丸山 雄樹, 光井 洋介, 西村 慎吾, 高本 篤, 和田 耕一郎, 谷本 竜太, 杉本 盛人, 小林 泰之, 渡部 昌実, 渡辺 豊彦, 那須 保友, 柳井 広之

    西日本泌尿器科   81 ( 4 )   461 - 466   2019年8月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    消化器癌を原発とする転移性尿管腫瘍の2例を経験したため報告する。【症例1】64歳、男性。2007年9月、下行結腸癌に対して下行結腸切除術を施行した。2014年、術後のCTで右尿管腫瘍を指摘され当科へ紹介となった。右尿管鏡検査時の生検病理結果より下行結腸癌の尿管転移と診断し、開腹腫瘍摘出術を行った。術後4ヵ月の時点で肝転移を認めたためTS-1 120mg内服療法を14ヵ月行い、様々な変更を経て現在はXELIRI + Bevacizumab療法8コース目を行っている。肝転移は緩徐に増大中である。【症例2】62歳、男性。2014年12月、多発胃癌に対して幽門側胃切除術、胆嚢摘出術およびRoux-en-Y再建術を施行し、術後TS-1 100mg内服療法を1年間行った。2017年、CTで右水腎症と尿管壁肥厚を指摘され、右尿管鏡検査を行うも狭窄が強く組織は採取できず、検査時の分腎尿細胞診はclass IVであった。再施行した右尿管鏡検査において狭窄尾側を生検したところ浸潤性尿路上皮癌の診断であったため、後腹膜鏡下右腎尿管摘除術を施行した。右尿管は腹膜およびS状結腸と著しく癒着し、漿膜ごと摘除した。病理結果は胃癌の右尿管転移であった。術後weekly PTX 100mgを開始したが骨転移の出現や腹膜転移の進行を認め、PTX + Ramucirumab 1コース施行した後に抗PD-1抗体180mgを5コース行ったが全身状態は徐々に悪化した。術後7ヵ月で緩和療法に移行し、術後11ヵ月で永眠された。転移性尿管腫瘍は術前生検しても診断が難しい場合がある。予後不良な疾患であり、悪性疾患の既往のある尿管腫瘍は転移性尿管腫瘍も鑑別に挙げる必要がある。(著者抄録)

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  • ロボット支援腹腔鏡下腎部分切除術後の腎癌尿管再発の1例

    角南 亮輔, 大岩 裕子, 高本 篤, 関戸 崇了, 佐久間 貴文, 和田里 章悟, 河村 香澄, 丸山 雄樹, 光井 洋介, 窪田 理沙, 定平 卓也, 片山 聡, 岩田 健宏, 西村 慎吾, 佐古 智子, 和田 耕一郎, 枝村 康平, 小林 泰之, 荒木 元朗, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   81 ( 4 )   482 - 482   2019年8月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 腎移植、明日への挑戦 ロボット自家腎移植

    荒木 元朗, 和田 耕一郎, 西村 慎吾, 窪田 理沙, 河村 香澄, 丸山 雄樹, 光井 洋介, 定平 卓也, 高本 篤, 佐古 智子, 枝村 康平, 小林 泰之, 石井 亜矢乃, 渡部 昌美, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   81 ( 3 )   314 - 320   2019年6月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    自家腎移植は本来摘出される腎臓を救済できる優れた手術だが、侵襲が大きく臨床応用の頻度は極めて少ない。2014年以降、傷の小さい低侵襲のロボット自家腎移植が北米を中心に臨床応用されている。当科ではまずブタでfeasibility studyを行いヒトへの臨床応用に移行した。対象は30代女性。左卵巣嚢腫破裂で緊急帝王切開および左付属器切除(出血量4L)。再出血のため左内腸骨動脈の血管塞栓術を施行。術後左尿管口より5cm頭側で長さ2.7cmの尿管狭窄に対し左尿管ステント留置。尿路造影にて左腎盂尿管移行部狭窄も疑われた。4年後、手術目的に当科を紹介されるも躊躇。さらに4年後、再診。創の小さいロボット自家腎移植を選択。傷も小さくステントフリーとなり患者は満足されている。ロボット自家腎移植は我々の症例も含め世界で4例報告されており、すべて距離の長い尿管狭窄に対して行われている。そうした症例に頻用されるileal ureterは腸管の尿の再吸収による代謝性アシドーシスなどが問題となり、Cr>2mg/dlでは禁忌である。Cr>2mg/dlの日本人は56万人も存在し、成人の179人に1人に相当することよりileal ureterを造設したときCr 2mg/dl以下でも、その患者が一生涯Cr 2mg/dl以下で過ごせる可能性は高くない。Boari flapは逆流防止がないもしくは困難で膀胱尿管逆流による尿路感染症などが問題となる。自家腎移植は尿の再吸収による代謝性アシドーシスがなく、逆流防止も可能な優れた手術である。ロボット自家腎移植は腎保存の新しい低侵襲手術である。(著者抄録)

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  • 経尿道的砕石術(TUL)を施行した小児腎結石症の1例

    佐久間貴文, 和田耕一郎, 本郷智拡, 前原貴典, 高本篤, 坪井一朗, 松尾聡子, 三井將雄, 和田里章悟, 河村香澄, 丸山雄樹, 光井洋介, 窪田理沙, 大岩裕子, 定平卓也, 西村慎吾, 佐古智子, 枝村康平, 小林泰之, 石井亜矢乃, 荒木元朗, 渡部昌実, 渡邉豊彦, 那須保友

    西日本泌尿器科   81 ( 2 )   243‐244 - 244   2019年4月

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    記述言語:日本語  

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  • 胚細胞腫瘍におけるブレオマイシン関連薬剤性肺障害の炎症性バイオマーカーの探索

    丸山 雄樹, 定平 卓也, 荒木 元朗, 光井 洋介, 西村 慎吾, 高本 篤, 佐古 智子, 和田 耕一郎, 枝村 康平, 小林 泰之, 渡部 昌実, 渡邉 豊彦, 那須 保友

    日本泌尿器科学会総会   107回   PP1 - 137   2019年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

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  • 胚細胞腫瘍に対する化学療法前の炎症性バイオマーカーと生存率の比較検討

    河村 香澄, 丸山 雄樹, 定平 卓也, 光井 洋介, 西村 慎吾, 高本 篤, 佐古 智子, 和田 耕一郎, 枝村 康平, 小林 泰之, 荒木 元朗, 渡辺 豊彦, 那須 保友

    日本泌尿器科学会総会   107回   OP - 248   2019年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

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  • ロボット支援腎部分切除術における切除皮質体積の重要性 切除組織からの3D再構築による精密測定を用いて

    光井 洋介, 定平 卓也, 荒木 元朗, 丸山 雄樹, 西村 慎吾, 高本 篤, 佐古 智子, 和田 耕一郎, 枝村 康平, 小林 泰之, 渡部 昌実, 渡邉 豊彦, 那須 保友

    日本泌尿器科学会総会   107回   AOP - 105   2019年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

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  • 前立腺全摘除術前における針生検病理標本second-lookの有用性

    定平 卓也, 丸山 雄樹, 光井 洋介, 西村 慎吾, 高本 篤, 佐古 智子, 和田 耕一郎, 枝村 康平, 小林 泰之, 荒木 元朗, 渡部 昌実, 渡邉 豊彦, 那須 保友

    日本泌尿器科学会総会   107回   PP1 - 070   2019年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

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  • 尿路性器感染症における遺伝子解析 前立腺生検後の急性前立腺炎における原因微生物の遺伝子解析

    和田 耕一郎, 定平 卓也, 荒木 元朗, 小林 泰之, 石井 亜矢乃, 渡邉 豊彦, 藤田 竜二, 竹中 皇, 那須 良次, 津川 昌也, 津島 知靖, 那須 保友, 公文 裕巳

    日本泌尿器科学会総会   107回   SY10 - 1   2019年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

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  • PNにおける腎機能温存は生命予後に寄与しているのか? 腎部分切除術における腎機能温存と心血管イベントの関連性の検討

    枝村 康平, 窪田 理沙, 大岩 裕子, 定平 卓也, 西村 慎吾, 高本 篤, 佐古 智子, 和田 耕一郎, 小林 泰之, 荒木 元朗, 渡部 昌実, 渡邉 豊彦, 那須 保友

    日本泌尿器科学会総会   107回   SY15 - 3   2019年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

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  • がん・生殖の現状と今後の展望地域ネットワークにおける泌尿器科医の役割は何か? 岡山大学病院におけるがん生殖医療の現状と課題

    佐古 智子, 杉本 盛人, 高山 修, 中塚 幹也, 和田 耕一郎, 小林 泰之, 荒木 元朗, 渡部 昌実, 渡辺 豊彦, 那須 保友

    日本泌尿器科学会総会   107回   SY20 - 4   2019年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

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  • QOL向上をめざした泌尿器がん治療 岡山大学病院におけるロボット支援腹腔鏡下膀胱全摘除術の経験

    小林 泰之, 三井 将雄, 西村 慎吾, 高本 篤, 佐古 智子, 和田 耕一郎, 枝村 康平, 荒木 元朗, 渡辺 豊彦, 那須 保友

    西日本泌尿器科   81 ( 2 )   122 - 127   2019年4月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    2018年にロボット支援腹腔鏡下膀胱全摘除術が保険収載され、今後本邦においても普及していくことが予想される。今回、当院にて膀胱全摘除術を行った102例(ORC35例、LRC52例、RARC15例)を対象に後方視的に検討を行った。検討項目は、手術時間、出血量、術後合併症、入院日数、術後の歩行開始日、術後疼痛、術後発熱とした。手術時間はRARCが最も短く、出血量はRARCが最も少なかった。術後の合併症はいずれの群においても約33%(全グレード)認めた。術後の歩行開始日、発熱が持続した期間は鏡視下手術群がORCと比して優れた結果であった。入院日数においては3群に有意な差はなかった。RARCはORCに比べ術後早期に回復が期待できるだけでなく、症例ごとの術後経過に差が少なく安定した手術成績が期待できる。(著者抄録)

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  • 第317回日本泌尿器科学会岡山地方会演題抄録 難治性過活動膀胱(OAB)に対する仙骨神経刺激療法(SNM)の初期経験と今後の治療戦略について

    渡辺豊彦, 大岩裕子, 佐久間貴文, 定平卓也, 石井亜矢乃, 佐古智子, 和田耕一郎, 枝村康平, 小林泰之, 荒木元朗, 那須保友, 横山光彦, 井上 雅, 小林知子

    西日本泌尿器科   81 ( 2 )   245   2019年4月

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    記述言語:日本語  

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  • 特集2:腹腔鏡手術~初心者に何をどう教えるべきか~:ロボット支援腹腔鏡下手術時代における腹腔鏡教育

    小林泰之, 和田耕一郎, 枝村康平, 荒木元朗, 渡辺豊彦, 那須保友

    Japanese Journal of Endourology   32 ( 1 )   28 - 31   2019年4月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • タゾバクタム/セフトロザンの尿路感染症における有用性

    和田耕一郎, 定平卓也, 山本満寿美, 石井亜矢乃, 渡邉豊彦, 那須保友

    日本化学療法学会雑誌   67 ( Supplement-A )   230   2019年3月

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    記述言語:日本語  

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  • 当科における尿中ESBL産生大腸菌の分離状況と薬剤感受性

    和田耕一郎, 山本満寿美, 三井將雄, 定平卓也, 石井亜矢乃, 渡邉豊彦, 那須保友

    日本化学療法学会雑誌   67 ( Supplement-A )   260   2019年3月

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  • 尿路感染症における多剤耐性の現状

    和田耕一郎, 定平卓也, 山本満寿美, 光畑律子, 石井亜矢乃, 狩山玲子, 渡邉豊彦, 那須保友

    感染症学雑誌   93   280‐281   2019年3月

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    記述言語:日本語  

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  • Azithromycinのヒト精巣上体組織への移行性の検討および部位特異的PK-PD解析による投与法の評価

    三井 將雄, 定平 卓也, 和田 耕一郎, 猪川 和朗, 山本 満寿美, 石井 亜矢乃, 渡邉 豊彦, 森川 則文, 那須 保友

    日本化学療法学会雑誌   67 ( 2 )   229 - 230   2019年3月

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    記述言語:日本語   出版者・発行元:(公社)日本化学療法学会  

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  • Azithromycinのヒト精巣上体組織への移行性の検討および部位特異的PK-PD解析による投与法の評価

    三井 將雄, 定平 卓也, 和田 耕一郎, 猪川 和朗, 山本 満寿美, 石井 亜矢乃, 渡邉 豊彦, 森川 則文, 那須 保友

    日本化学療法学会雑誌   67 ( 2 )   229 - 230   2019年3月

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    記述言語:日本語   出版者・発行元:(公社)日本化学療法学会  

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  • 高位精巣摘除術後14年後に再発をきたしたStage I期セミノーマの1例(VERY LATE RELAPSE AND MANAGEMENT OF CLINICAL STAGE I SEMINOMA AFTER ORCHIECTOMY: A CASE REPORT)

    和田里 章悟, 定平 卓也, 高本 篤, 丸山 雄樹, 光井 洋介, 和田 耕一郎, 谷本 竜太, 杉本 盛人, 小林 泰之, 荒木 元朗, 渡邉 昌実, 渡邉 豊彦, 那須 保友, 柳井 広之

    西日本泌尿器科   81 ( 1 )   45 - 48   2019年2月

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    記述言語:英語   出版者・発行元:西日本泌尿器科学会  

    Stage I期セミノーマの手術後には補助治療を行わず経過観察とするsurveillanceが現在の主流となっており、再発率は13〜20%と言われている。補助治療の有無によらず、再発の大半は手術後2年以内が占める。以降の再発は晩期再発と呼ばれ区別されているが、そのリスク因子は明確になっていない。手術後5年以降の再発は0.8%〜2.5%と報告されており、術後10年を超えての再発はさらに稀である。今回、我々は高位精巣摘除術14年後に発生したセミノーマ晩期再発を経験したので、若干の文献的考察を加え報告する。症例は40代、男性。右下腿浮腫、総腸骨リンパ節の腫大、骨盤内腫瘤を指摘され当科紹介受診した。患者は前医受診の14年前に右精巣腫瘍に対し高位精巣摘除術を行われており、セミノーマpT2N0M0 Stage IBとして術後6年間のフォローの後に終診となっていた。当科受診時にはLDH 667 U/l(120〜240U/l)、β-hCG 17.5mIU/l(<0.5mIU/l)と高値を認め、骨盤内腫瘤生検によりセミノーマの再発と診断、救済化学療法としてBEP3コース、VeIP療法を3コース施行した。VeIP療法施行後、骨盤内残存腫瘤の切除を行った。摘出標本は壊死組織のみでviable cellは認めず、術後7ヵ月時点では局所再発、転移は認めていない。(著者抄録)

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  • 生体腎移植における術前抗ドナー抗体が治療成績にもたらす影響

    丸山 雄樹, 荒木 元朗, 和田 耕一郎, 坪井 一朗, 河村 香澄, 光井 洋介, 窪田 理沙, 西村 慎吾, 山下 里美, 渡邉 豊彦, 那須 保友

    日本臨床腎移植学会プログラム・抄録集   52回   170 - 170   2019年2月

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    記述言語:日本語   出版者・発行元:(一社)日本臨床腎移植学会  

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  • 当院の生体腎移植におけるリツキシマブ投与の効果と有害事象

    河村 香澄, 荒木 元朗, 丸山 雄樹, 坪井 一朗, 光井 洋介, 窪田 理沙, 西村 慎吾, 和田 耕一郎, 山下 里美, 小林 泰之, 渡邉 豊彦, 那須 保友

    日本臨床腎移植学会プログラム・抄録集   52回   261 - 261   2019年2月

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    記述言語:日本語   出版者・発行元:(一社)日本臨床腎移植学会  

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  • 当科における腹腔鏡下と後腹膜鏡下ドナー腎採取術の比較検討

    西村 慎吾, 荒木 元朗, 小林 泰之, 山下 里美, 坪井 一朗, 河村 香澄, 丸山 雄樹, 光井 洋介, 和田 耕一郎, 渡辺 豊彦, 那須 保友

    日本臨床腎移植学会プログラム・抄録集   52回   217 - 217   2019年2月

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    記述言語:日本語   出版者・発行元:(一社)日本臨床腎移植学会  

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  • 当院で施行した先行的腎移植の検討

    坪井一朗, 荒木元朗, 河村香澄, 丸山雄樹, 窪田理沙, 定平卓也, 西村慎吾, 和田耕一郎, 小林泰之, 渡邉豊彦, 北川正史, 田邊克幸, 杉山斉, 和田淳, 那須保友

    日本臨床腎移植学会プログラム・抄録集   52nd   142 - 142   2019年2月

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    記述言語:日本語   出版者・発行元:(一社)日本臨床腎移植学会  

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  • 高位精巣摘除術後14年後に再発をきたしたStage I期セミノーマの1例(VERY LATE RELAPSE AND MANAGEMENT OF CLINICAL STAGE I SEMINOMA AFTER ORCHIECTOMY: A CASE REPORT)

    和田里 章悟, 定平 卓也, 高本 篤, 丸山 雄樹, 光井 洋介, 和田 耕一郎, 谷本 竜太, 杉本 盛人, 小林 泰之, 荒木 元朗, 渡邉 昌実, 渡邉 豊彦, 那須 保友, 柳井 広之

    西日本泌尿器科   81 ( 1 )   45 - 48   2019年2月

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    記述言語:英語   出版者・発行元:西日本泌尿器科学会  

    Stage I期セミノーマの手術後には補助治療を行わず経過観察とするsurveillanceが現在の主流となっており、再発率は13〜20%と言われている。補助治療の有無によらず、再発の大半は手術後2年以内が占める。以降の再発は晩期再発と呼ばれ区別されているが、そのリスク因子は明確になっていない。手術後5年以降の再発は0.8%〜2.5%と報告されており、術後10年を超えての再発はさらに稀である。今回、我々は高位精巣摘除術14年後に発生したセミノーマ晩期再発を経験したので、若干の文献的考察を加え報告する。症例は40代、男性。右下腿浮腫、総腸骨リンパ節の腫大、骨盤内腫瘤を指摘され当科紹介受診した。患者は前医受診の14年前に右精巣腫瘍に対し高位精巣摘除術を行われており、セミノーマpT2N0M0 Stage IBとして術後6年間のフォローの後に終診となっていた。当科受診時にはLDH 667 U/l(120〜240U/l)、β-hCG 17.5mIU/l(<0.5mIU/l)と高値を認め、骨盤内腫瘤生検によりセミノーマの再発と診断、救済化学療法としてBEP3コース、VeIP療法を3コース施行した。VeIP療法施行後、骨盤内残存腫瘤の切除を行った。摘出標本は壊死組織のみでviable cellは認めず、術後7ヵ月時点では局所再発、転移は認めていない。(著者抄録)

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  • 先行的腎移植における健康関連QOLの検討

    光井 洋介, 荒木 元朗, 和田 耕一郎, 山下 里美, 河村 香澄, 丸山 雄樹, 坪井 一朗, 窪田 理沙, 有吉 勇一, 西村 慎吾, 那須 保友

    日本臨床腎移植学会プログラム・抄録集   52回   215 - 215   2019年2月

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    記述言語:日本語   出版者・発行元:(一社)日本臨床腎移植学会  

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  • 当科における腹腔鏡下と後腹膜鏡下ドナー腎採取術の比較検討

    西村 慎吾, 荒木 元朗, 小林 泰之, 山下 里美, 坪井 一朗, 河村 香澄, 丸山 雄樹, 光井 洋介, 和田 耕一郎, 渡辺 豊彦, 那須 保友

    日本臨床腎移植学会プログラム・抄録集   52回   217 - 217   2019年2月

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    記述言語:日本語   出版者・発行元:(一社)日本臨床腎移植学会  

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  • 当院の生体腎移植におけるリツキシマブ投与の効果と有害事象

    河村 香澄, 荒木 元朗, 丸山 雄樹, 坪井 一朗, 光井 洋介, 窪田 理沙, 西村 慎吾, 和田 耕一郎, 山下 里美, 小林 泰之, 渡邉 豊彦, 那須 保友

    日本臨床腎移植学会プログラム・抄録集   52回   261 - 261   2019年2月

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    記述言語:日本語   出版者・発行元:(一社)日本臨床腎移植学会  

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  • 先行的腎移植における健康関連QOLの検討

    光井 洋介, 荒木 元朗, 和田 耕一郎, 山下 里美, 河村 香澄, 丸山 雄樹, 坪井 一朗, 窪田 理沙, 有吉 勇一, 西村 慎吾, 那須 保友

    日本臨床腎移植学会プログラム・抄録集   52回   215 - 215   2019年2月

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    記述言語:日本語   出版者・発行元:(一社)日本臨床腎移植学会  

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  • 生体腎移植における術前抗ドナー抗体が治療成績にもたらす影響

    丸山 雄樹, 荒木 元朗, 和田 耕一郎, 坪井 一朗, 河村 香澄, 光井 洋介, 窪田 理沙, 西村 慎吾, 山下 里美, 渡邉 豊彦, 那須 保友

    日本臨床腎移植学会プログラム・抄録集   52回   170 - 170   2019年2月

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    記述言語:日本語   出版者・発行元:(一社)日本臨床腎移植学会  

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  • 女性過活動膀胱に対する牛車腎気丸の効果と冷えとの関係について

    松本 裕子, 石井 亜矢乃, 山本 満寿美, 松尾 聡子, 佐古 智子, 荒木 元朗, 渡邉 豊彦, 那須 保友, 井上 雅, 荒木 詞奈子, 石戸 則孝, 高本 均

    西日本泌尿器科   81 ( 1 )   12 - 16   2019年2月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    牛車腎気丸には過活動膀胱症状を改善する効果があることが知られているが、末梢血流増加作用もある。女性過活動膀胱に対し牛車腎気丸を投与し、治療効果と冷えとの関係を検討した。成人女性過活動膀胱患者56例を対象とし、TJ-107ツムラ牛車腎気丸エキス顆粒(牛車腎気丸)1日7.5gを分3で4週間投与し、治療前、治療4週目に過活動膀胱質問票(Overactive Bladder Symptom Score;OABSS)、冷えに関する質問票で症状を確認し、非接触型赤外線温度計で体表温度(手掌、恥骨上、足蹠)を測定した。他の過活動膀胱治療薬を内服している場合は変更せず継続した。平均年齢は35〜90歳(平均64.1±13.8歳)で、OABSSは治療前7.57±3.36、後6.73±3.50と有意に改善した(p=0.006)。治療前後で体表温度の有意な上昇はみられなかったが、治療前に冷えを自覚した36例(64.3%、平均65.1±13.8歳)ではOABSSが治療前7.94±3.65、後6.86±3.61と有意に改善し(p=0.004)、治療前に冷えを自覚しなかった20例(35.7%、平均62.3±13.6歳)では改善傾向はあったが有意差はなかった。このことから、治療前に冷えの自覚があるほうが、自覚のない症例に比べて牛車腎気丸による治療効果が期待でき、治療の指標になりうると考えられた。(著者抄録)

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  • 尿道腫瘤を認めたメトトレキサート関連リンパ増殖性疾患の1例

    河村香澄, 丸山雄樹, 定平卓也, 光井洋介, 西村慎吾, 高本篤, 甲斐誠二, 和田耕一郎, 谷本竜太, 杉本盛人, 小林泰之, 荒木元朗, 渡部昌実, 渡辺豊彦, 那須保友, 田中健大

    西日本泌尿器科   80 ( 12 )   678‐683 - 683   2018年12月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    症例は64歳、女性。2007年に関節リウマチの診断を受け、2008年よりメトトレキサート(MTX)内服中であった。2015年に繰り返す発熱で近医を受診。CTで子宮頸癌および両肺多発転移を疑われた。当院婦人科で子宮頸部パンチ生検を施行するも悪性所見なく、肺結節も自然消退したため経過観察となっていた。2017年2月、症状が再燃し近医を受診した。CTで左下葉結節影、右肺門リンパ節腫脹、腹腔内リンパ節腫脹および左腎盂拡張を認め、PET-CTで左腎門部リンパ節、尿道、上咽頭に高集積を認めた。MTX関連リンパ増殖性疾患(MTX-LPD)を疑い、施行した上咽頭生検はHodgkinリンパ腫の像を呈し、EBER-ISH陽性であった。MTX中止後は左尿管病変および尿道病変に著変はないものの、肺腫瘤とリンパ節は縮小傾向で現在経過観察中である。(著者抄録)

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  • 超細径HDIGスコープを用いた直視下腎杯穿刺とPCNLの初期成績

    和田耕一郎, 公文裕巳, 谷本竜太, 荒木元朗, 本郷智拡, 三井將雄, 松尾聡子, 坪井一朗, 佐久間貴文, 和田里章悟, 河村香澄, 丸山雄樹, 光井洋介, 前原貴典, 窪田理沙, 大岩裕子, 定平卓也, 岩田健宏, 西村慎吾, 高本篤, 佐古智子, 枝村康平, 杉本盛人, 小林泰之, 石井亜矢乃, 渡部昌実, 渡邉豊彦, 那須保友

    西日本泌尿器科   80 ( 12 )   700 - 700   2018年12月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • JUA Future Vision 2018:4-1海外泌尿器科organization(AUA, EAU,SIU,UAA)との連携とFacultyとしての参画のありかた 4-2個々の国との連携のありかた(これまでの交流を将来に生かす可能性を考えて) 4-3日泌総会、東部、中部、西日本総会等学術集会での国際化のありかた

    冨田善彦, 久米春喜, 藤井靖久, 木下秀文, 齋藤和英, 那須保友, 安井孝周, 頴川 晋, 立神勝則, 荒木元朗, 柿崎秀宏, 井川 掌, 巴ひかる, 橘田岳也

    日本泌尿器科学会雑誌   109   44 - 57   2018年11月

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    記述言語:日本語  

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  • 再発性尿路感染症に対するプロバイオティクスの有効性

    定平卓也, 和田耕一郎, 三井將雄, 石井亜矢乃, 荒木元朗, 渡部昌実, 渡邉豊彦, 那須保友

    西日本泌尿器科   80 ( 10 )   505‐509 - 509   2018年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    尿路感染症に対する治療として中心的な役割を果たしてきた抗菌化学療法は大きな転機を迎えている。世界的な時流として耐性菌の問題や医療経済的にも抗菌薬の使用量を減少させる方向に移行している。尿路感染症においても例外ではなく、そのマネジメントに関して治療法の見直しだけでなく予防の重要性についての議論がなされている。そのうち、これまでに様々な臨床研究において有効性が確認されつつあるプロバイオティクスが抗菌薬とは異なる尿路感染症対策として注目されている。健常な閉経前の女性の腟内においては、基本的に乳酸菌が細菌叢(フローラ)を形成しているが、月経中や閉経後は乳酸菌が減少して腸内細菌が腟内に定着することとなる。その腟がリザーバーとなって細菌を供給し、頻繁に尿路の逆行性感染を引き起こすと考えられている。すなわち、腟内を乳酸菌中心のフローラに保つことで尿路感染症の再発(反復)リスクが低くなることが期待でき、実際にプロバイオティクスを用いた多くの研究が行われている。我々が現在行っている乳酸菌製剤のほか、プロバイオティクスの経口摂取が腟内フローラに与える影響に関しても検証が進んでおり、プロバイオティクスが尿路感染症の予防や治療に寄与することが期待されている。(著者抄録)

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  • Azithromycinのヒト精巣上体組織への移行性の検討および部位特異的PK‐PD解析による投与法の評価

    三井將雄, 定平卓也, 和田耕一郎, 猪川和朗, 山本満寿美, 石井亜矢乃, 渡邉豊彦, 森川則文, 那須保友

    日本化学療法学会西日本支部総会プログラム・講演抄録   66th   157   2018年10月

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  • 尿路上皮癌リンパ節再発を来した血液透析患者にGC療法が奏功した一例

    川野 香, 和田里 章悟, 谷本 竜太, 三井 将雄, 西村 慎吾, 佐古 智子, 大西 章史, 和田 耕一郎, 小林 泰之, 荒木 元朗, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 増刊 )   186 - 186   2018年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 腹腔鏡下膀胱全摘術および回腸導管による尿路変更後に空腸導管症候群様の症状を来した1例

    松尾 聡子, 杉本 盛人, 西村 慎吾, 河村 香澄, 大岩 裕子, 岩田 健宏, 高本 篤, 大西 章史, 北川 正史, 寺石 文則, 小林 泰之, 渡辺 豊彦, 那須 保友

    西日本泌尿器科   80 ( 増刊 )   180 - 180   2018年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • がん免疫療法、明日への挑戦 前立腺癌における免疫遺伝子治療

    定平 卓也, 那須 保友

    西日本泌尿器科   80 ( 増刊 )   142 - 142   2018年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 再発性尿路感染症に対するプロバイオティクスの有効性

    定平 卓也, 和田 耕一郎, 三井 將雄, 石井 亜矢乃, 荒木 元朗, 渡部 昌実, 渡邉 豊彦, 那須 保友

    西日本泌尿器科 = The Nishinihon journal of urology   80 ( 10 )   505 - 509   2018年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    尿路感染症に対する治療として中心的な役割を果たしてきた抗菌化学療法は大きな転機を迎えている。世界的な時流として耐性菌の問題や医療経済的にも抗菌薬の使用量を減少させる方向に移行している。尿路感染症においても例外ではなく、そのマネジメントに関して治療法の見直しだけでなく予防の重要性についての議論がなされている。そのうち、これまでに様々な臨床研究において有効性が確認されつつあるプロバイオティクスが抗菌薬とは異なる尿路感染症対策として注目されている。健常な閉経前の女性の腟内においては、基本的に乳酸菌が細菌叢(フローラ)を形成しているが、月経中や閉経後は乳酸菌が減少して腸内細菌が腟内に定着することとなる。その腟がリザーバーとなって細菌を供給し、頻繁に尿路の逆行性感染を引き起こすと考えられている。すなわち、腟内を乳酸菌中心のフローラに保つことで尿路感染症の再発(反復)リスクが低くなることが期待でき、実際にプロバイオティクスを用いた多くの研究が行われている。我々が現在行っている乳酸菌製剤のほか、プロバイオティクスの経口摂取が腟内フローラに与える影響に関しても検証が進んでおり、プロバイオティクスが尿路感染症の予防や治療に寄与することが期待されている。(著者抄録)

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  • 腎尿管全摘術後ピラルビシン膀胱内注入療法の膀胱内再発予防効果

    前原 貴典, 小林 泰之, 江原 伸, 那須 良次, 宮地 禎幸, 竹中 皇, 津島 知靖, 明比 直樹, 佐々木 克己, 高本 篤, 和田 耕一郎, 荒木 元朗, 渡邉 豊彦, 那須 保友

    日本癌治療学会学術集会抄録集   56回   P36 - 3   2018年10月

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    記述言語:英語   出版者・発行元:(一社)日本癌治療学会  

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  • ロボット支援下腎部分切除におけるearly unclamp法の術後腎機能に対する有用性の検討

    和田里 章悟, 谷本 竜太, 丸山 雄樹, 光井 洋介, 前原 貴典, 西村 慎吾, 高本 篤, 杉本 盛人, 和田 耕一郎, 小林 泰之, 荒木 元朗, 渡辺 豊彦, 那須 保友

    西日本泌尿器科   80 ( 増刊 )   189 - 189   2018年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • ロボット支援下腎部分切除におけるearly unclamp法の術後腎機能に対する有用性の検討

    和田里 章悟, 谷本 竜太, 丸山 雄樹, 光井 洋介, 前原 貴典, 西村 慎吾, 高本 篤, 杉本 盛人, 和田 耕一郎, 小林 泰之, 荒木 元朗, 渡辺 豊彦, 那須 保友

    西日本泌尿器科   80 ( 増刊 )   189 - 189   2018年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • がん免疫療法、明日への挑戦 前立腺癌における免疫遺伝子治療

    定平 卓也, 那須 保友

    西日本泌尿器科   80 ( 増刊 )   142 - 142   2018年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 腎移植、明日への挑戦 ロボット自家腎移植

    荒木 元朗, 和田 耕一郎, 小林 泰之, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 増刊 )   134 - 134   2018年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 腎癌に対するロボット支援腹腔鏡下腎部分切除術後の腹腔内再発の1例

    河村 香澄, 小林 泰之, 岩田 健宏, 西村 慎吾, 高本 篤, 佐古 智子, 和田 耕一郎, 杉本 盛人, 谷本 竜太, 荒木 元朗, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 増刊 )   190 - 190   2018年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 移植腎における腎細胞癌に対して造影CTガイド下経皮的凍結療法を施行した1例

    坪井 一朗, 荒木 元朗, 藤原 寛康, 生口 俊浩, 有地 直子, 窪田 理沙, 西村 慎吾, 和田 耕一郎, 小林 泰之, 渡邉 豊彦, 椎名 浩昭, 金澤 右, 那須 保友

    西日本泌尿器科   80 ( 増刊 )   165 - 165   2018年10月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 自然消失した膀胱炎症性偽腫瘍の1例

    丸山雄樹, 高本篤, 光井洋介, 定平卓也, 岩田健宏, 西村慎吾, 甲斐誠二, 和田耕一朗, 谷本竜太, 杉本盛人, 小林泰之, 荒木元朗, 渡辺昌実, 渡邉豊彦, 那須保友

    西日本泌尿器科   80 ( 9 )   465‐468 - 468   2018年9月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    症例は65歳、女性。繰り返す膀胱炎様症状で前医を受診。膀胱鏡検査で膀胱後壁に腫瘤性病変を認めた。尿細胞診は陰性、CT、MRIでは炎症波及による2次性膀胱腫瘤の可能性を指摘され、経尿道的膀胱腫瘍生検を行うも病理結果では悪性所見なく炎症性変化との診断であった。症状の改善なく本年2月に当院紹介受診となった。各種自己免疫疾患の検査はいずれも陰性であった。CT、MRIを再検したがやはり炎症性腫瘤を疑われ、再度経尿道的膀胱腫瘍生検を試みた。膀胱内を観察すると腫瘤性病変は消退しており前回生検時の瘢痕を残すのみであった。瘢痕部位を再度生検し病理検査に提出したが結果は炎症所見のみで悪性所見は認めず炎症性偽腫瘍と診断した。生検後は大幅な症状の改善を認め、さらなる侵襲的な検査は行わず、経過観察とした。術後15ヵ月現在、症状の再燃を認めていない。(著者抄録)

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  • 前立腺に発生したSFT(Solitary fibrous tumor)の1例

    本郷智拡, 高本篤, 三井將雄, 松尾聡子, 坪井一朗, 佐久間貴文, 和田里章悟, 河村香澄, 丸山雄樹, 光井洋介, 前原貴典, 窪田理沙, 大岩裕子, 定平卓也, 西村慎吾, 佐古智子, 和田耕一郎, 谷本竜太, 杉本盛人, 小林泰之, 石井亜矢乃, 荒木元朗, 渡部昌実, 渡邉豊彦, 那須保友

    西日本泌尿器科   80 ( 9 )   497 - 497   2018年9月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 排尿障害を伴う女性尿道憩室に対して手術治療を行った1例

    松尾聡子, 杉本盛人, 佐久間貴文, 坪井一朗, 本郷智拡, 三井將雄, 河村香澄, 和田里章悟, 丸山雄樹, 光井洋介, 定平卓也, 前原貴典, 大岩裕子, 西村慎吾, 高本篤, 佐古智子, 和田耕一郎, 谷本竜太, 小林泰之, 荒木元朗, 石井亜矢乃, 渡部昌実, 渡邉豊彦, 那須保友, 中村あや, 津島知靖

    西日本泌尿器科   80 ( 9 )   495 - 495   2018年9月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 自然消失した膀胱炎症性偽腫瘍の1例

    丸山 雄樹, 高本 篤, 光井 洋介, 定平 卓也, 岩田 健宏, 西村 慎吾, 甲斐 誠二, 和田 耕一朗, 谷本 竜太, 杉本 盛人, 小林 泰之, 荒木 元朗, 渡辺 昌実, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 9 )   465 - 468   2018年9月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    症例は65歳、女性。繰り返す膀胱炎様症状で前医を受診。膀胱鏡検査で膀胱後壁に腫瘤性病変を認めた。尿細胞診は陰性、CT、MRIでは炎症波及による2次性膀胱腫瘤の可能性を指摘され、経尿道的膀胱腫瘍生検を行うも病理結果では悪性所見なく炎症性変化との診断であった。症状の改善なく本年2月に当院紹介受診となった。各種自己免疫疾患の検査はいずれも陰性であった。CT、MRIを再検したがやはり炎症性腫瘤を疑われ、再度経尿道的膀胱腫瘍生検を試みた。膀胱内を観察すると腫瘤性病変は消退しており前回生検時の瘢痕を残すのみであった。瘢痕部位を再度生検し病理検査に提出したが結果は炎症所見のみで悪性所見は認めず炎症性偽腫瘍と診断した。生検後は大幅な症状の改善を認め、さらなる侵襲的な検査は行わず、経過観察とした。術後15ヵ月現在、症状の再燃を認めていない。(著者抄録)

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  • 前立腺に発生したSFT(Solitary fibrous tumor)の1例

    本郷 智拡, 高本 篤, 三井 將雄, 松尾 聡子, 坪井 一朗, 佐久間 貴文, 和田里 章悟, 河村 香澄, 丸山 雄樹, 光井 洋介, 前原 貴典, 窪田 理沙, 大岩 裕子, 定平 卓也, 西村 慎吾, 佐古 智子, 和田 耕一郎, 谷本 竜太, 杉本 盛人, 小林 泰之, 石井 亜矢乃, 荒木 元朗, 渡部 昌実, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 9 )   497 - 497   2018年9月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 腎移植患者における24時間蓄尿から見る1日塩分・蛋白摂取量の意義

    光井 洋介, 荒木 元朗, 丸山 雄樹, 坪井 一朗, 河村 香澄, 窪田 理沙, 西村 慎吾, 和田 耕一郎, 小林 泰之, 渡辺 豊彦, 北川 正史, 田邊 克之, 杉山 斉, 和田 淳, 那須 保友

    移植   53 ( 総会臨時 )   475 - 475   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • リツキシマブは腎移植レシピエントの周術期細菌感染症のリスクになるか

    西村 慎吾, 和田 耕一郎, 荒木 元朗, 山下 里美, 坪井 一朗, 河村 香澄, 丸山 雄樹, 光井 洋介, 窪田 理沙, 定平 卓也, 谷本 竜太, 小林 泰之, 渡邉 豊彦, 那須 保友

    移植   53 ( 総会臨時 )   436 - 436   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 生体腎移植におけるリツキシマブ投与の効果と有害事象

    河村 香澄, 荒木 元朗, 丸山 雄樹, 坪井 一朗, 光井 洋介, 窪田 理沙, 西村 真吾, 和田 耕一郎, 小林 泰之, 渡邉 豊彦, 那須 保友, 山下 里美, 北川 正史, 田邉 克幸, 杉山 斉, 和田 淳

    移植   53 ( 総会臨時 )   481 - 481   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 鰓耳腎症候群の小児に対する生体腎移植の1例

    窪田 理沙, 荒木 元朗, 和田 耕一郎, 河村 香澄, 丸山 雄樹, 光井 洋介, 西村 慎吾, 小林 泰之, 渡邉 豊彦, 那須 保友, 宮井 貴之, 北川 正史, 田邊 克幸, 和田 淳

    移植   53 ( 総会臨時 )   434 - 434   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 生体腎移植レシピエントにおけるCT値を用いた骨密度の変化に関する検討

    丸山 雄樹, 荒木 元朗, 和田 耕一郎, 坪井 一朗, 河村 香澄, 光井 洋介, 窪田 理沙, 西村 慎吾, 小林 泰之, 渡邊 豊彦, 北川 正史, 田邊 克幸, 杉山 斉, 和田 淳, 那須 保友

    移植   53 ( 総会臨時 )   490 - 490   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 生体腎移植におけるリツキシマブ投与の効果と有害事象

    河村 香澄, 荒木 元朗, 丸山 雄樹, 坪井 一朗, 光井 洋介, 窪田 理沙, 西村 真吾, 和田 耕一郎, 小林 泰之, 渡邉 豊彦, 那須 保友, 山下 里美, 北川 正史, 田邉 克幸, 杉山 斉, 和田 淳

    移植   53 ( 総会臨時 )   481 - 481   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 鰓耳腎症候群の小児に対する生体腎移植の1例

    窪田 理沙, 荒木 元朗, 和田 耕一郎, 河村 香澄, 丸山 雄樹, 光井 洋介, 西村 慎吾, 小林 泰之, 渡邉 豊彦, 那須 保友, 宮井 貴之, 北川 正史, 田邊 克幸, 和田 淳

    移植   53 ( 総会臨時 )   434 - 434   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 生体腎移植レシピエントにおけるCT値を用いた骨密度の変化に関する検討

    丸山 雄樹, 荒木 元朗, 和田 耕一郎, 坪井 一朗, 河村 香澄, 光井 洋介, 窪田 理沙, 西村 慎吾, 小林 泰之, 渡邊 豊彦, 北川 正史, 田邊 克幸, 杉山 斉, 和田 淳, 那須 保友

    移植   53 ( 総会臨時 )   490 - 490   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 排尿障害を伴う女性尿道憩室に対して手術治療を行った1例

    松尾 聡子, 杉本 盛人, 佐久間 貴文, 坪井 一朗, 本郷 智拡, 三井 將雄, 河村 香澄, 和田里 章悟, 丸山 雄樹, 光井 洋介, 定平 卓也, 前原 貴典, 大岩 裕子, 西村 慎吾, 高本 篤, 佐古 智子, 和田 耕一郎, 谷本 竜太, 小林 泰之, 荒木 元朗, 石井 亜矢乃, 渡部 昌実, 渡邉 豊彦, 那須 保友, 中村 あや, 津島 知靖

    西日本泌尿器科   80 ( 9 )   495 - 495   2018年9月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • リツキシマブは腎移植レシピエントの周術期細菌感染症のリスクになるか

    西村 慎吾, 和田 耕一郎, 荒木 元朗, 山下 里美, 坪井 一朗, 河村 香澄, 丸山 雄樹, 光井 洋介, 窪田 理沙, 定平 卓也, 谷本 竜太, 小林 泰之, 渡邉 豊彦, 那須 保友

    移植   53 ( 総会臨時 )   436 - 436   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 腎移植患者における24時間蓄尿から見る1日塩分・蛋白摂取量の意義

    光井 洋介, 荒木 元朗, 丸山 雄樹, 坪井 一朗, 河村 香澄, 窪田 理沙, 西村 慎吾, 和田 耕一郎, 小林 泰之, 渡辺 豊彦, 北川 正史, 田邊 克之, 杉山 斉, 和田 淳, 那須 保友

    移植   53 ( 総会臨時 )   475 - 475   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)日本移植学会  

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  • 尿路感染を契機とした化膿性脊椎炎の3例

    森 聰博, 和田 耕一郎, 定平 卓也, 西村 慎吾, 藤尾 圭, 高本 篤, 堀川 雄平, 谷本 竜太, 杉本 盛人, 小林 泰之, 佐々木 克己, 荒木 元朗, 渡部 昌実, 江原 伸, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 8 )   428 - 433   2018年8月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    化膿性脊椎炎は尿路感染症や尿性敗血症(ウロセプシス)が原因の一つとなる。今回、尿路感染症の治療経過中に発症し、保存的加療により治癒した化膿性脊椎炎の3例を経験した。いずれの症例も腎盂腎炎に対する抗菌薬の投与中に腰背部痛が遷延もしくは増強し、発症から9日以上経過した複数回目のMRIで診断し得た。尿路感染症治療中に持続、または増強する背部痛、腰痛には注意が必要であり、化膿性脊椎炎を疑って複数回の画像診断を行う必要があると考えられた。(著者抄録)

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  • 尿路感染を契機とした化膿性脊椎炎の3例

    森 聰博, 和田 耕一郎, 定平 卓也, 西村 慎吾, 藤尾 圭, 高本 篤, 堀川 雄平, 谷本 竜太, 杉本 盛人, 小林 泰之, 佐々木 克己, 荒木 元朗, 渡部 昌実, 江原 伸, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 8 )   428 - 433   2018年8月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    化膿性脊椎炎は尿路感染症や尿性敗血症(ウロセプシス)が原因の一つとなる。今回、尿路感染症の治療経過中に発症し、保存的加療により治癒した化膿性脊椎炎の3例を経験した。いずれの症例も腎盂腎炎に対する抗菌薬の投与中に腰背部痛が遷延もしくは増強し、発症から9日以上経過した複数回目のMRIで診断し得た。尿路感染症治療中に持続、または増強する背部痛、腰痛には注意が必要であり、化膿性脊椎炎を疑って複数回の画像診断を行う必要があると考えられた。(著者抄録)

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  • 膵がん進展に導く膵がん細胞 間質線維芽細胞クロストークを介在する分泌性S100A11 受容体RAGE連携の役割

    光井 洋介, 山本 健一, Sumardika I Wayan, 木下 理恵, 村田 等, 二見 淳一郎, 高松 仁, 山本 靖彦, 西堀 正洋, 豊岡 伸一, 渡部 昌実, 那須 保友, 阪口 政清

    日本がん免疫学会総会プログラム・抄録集   22回   156 - 156   2018年7月

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    記述言語:日本語   出版者・発行元:日本がん免疫学会  

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  • 西日本泌尿器科と私

    那須保友

    西日本泌尿器科   80 ( 7 )   377 - 379   2018年7月

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    記述言語:日本語  

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  • 転移性尿管腫瘍の2例

    河村 香澄, 杉本 盛人, 小林 宏州, 和田里 章悟, 丸山 雄樹, 光井 洋介, 前原 貴典, 大岩 裕子, 定平 卓也, 岩田 健宏, 西村 慎吾, 高本 篤, 甲斐 誠二, 和田 耕一郎, 谷本 竜太, 荒木 元朗, 小林 泰之, 石井 亜矢乃, 渡部 昌実, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 5 )   242 - 242   2018年5月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 【遺伝子治療の新局面】 悪性腫瘍 前立腺癌(Ad-REIC) Ad-REICによる遺伝子治療の現状

    定平 卓也, 那須 保友

    医学のあゆみ   265 ( 5 )   422 - 428   2018年5月

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    記述言語:日本語   出版者・発行元:医歯薬出版(株)  

    泌尿器科領域においては,癌ワクチン効果を期待した種々の前立腺癌治療薬の開発が中心課題のひとつとなっている.著者らは,これまでに癌抑制・治療遺伝子REICを発現するアデノウイルスベクター(Ad-REIC)を臨床開発し,難治性前立腺癌に対するin-situ遺伝子治療を実施し,その抗腫瘍効果を実証してきた.この治療効果は,Ad-REIC製剤を用いた局所遺伝子治療による癌細胞の選択的細胞死と抗癌免疫の活性化による相乗効果増強作用(自己癌ワクチン化)に基づくと考えられている.本稿では,Ad-REIC製剤を用いた難治性前立腺癌の治療について,最近の研究結果も交えつつ解説する.(著者抄録)

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  • 岡山大学におけるロボット支援腹腔鏡下前立腺全摘除術の治療成績

    谷本 竜太, 小林 宏州, 河村 香澄, 和田里 章悟, 丸山 雄樹, 光井 洋介, 前原 貴典, 大岩 裕子, 定平 卓也, 岩田 健宏, 西村 慎吾, 高本 篤, 松本 裕子, 甲斐 誠二, 和田 耕一郎, 杉本 盛人, 荒木 元朗, 小林 泰之, 石井 亜矢乃, 渡部 昌実, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 5 )   246 - 246   2018年5月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 耐性菌時代の尿路感染症対策-尿路感染症の予防- Probioticsによる尿路感染症予防

    和田 耕一郎, 定平 卓也, 山本 満寿美, 光畑 律子, 石井 亜矢乃, 渡邉 豊彦, 那須 保友

    日本化学療法学会雑誌   66 ( 3 )   421 - 421   2018年5月

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    記述言語:日本語   出版者・発行元:(公社)日本化学療法学会  

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  • 耐性菌時代の尿路感染症対策-尿路感染症の予防- Probioticsによる尿路感染症予防

    和田 耕一郎, 定平 卓也, 山本 満寿美, 光畑 律子, 石井 亜矢乃, 渡邉 豊彦, 那須 保友

    感染症学雑誌   92 ( 3 )   430 - 430   2018年5月

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    記述言語:日本語   出版者・発行元:(一社)日本感染症学会  

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  • 岡山大学病院泌尿器科における腹腔鏡下尿膜管摘出術の治療経験

    前原 貴典, 杉本 盛人, 和田里 章悟, 河村 香澄, 小林 宏州, 丸山 雄樹, 光井 洋介, 大岩 裕子, 定平 卓也, 岩田 健宏, 西村 慎吾, 高本 篤, 甲斐 誠二, 和田 耕一郎, 谷本 竜太, 小林 泰之, 石井 亜矢乃, 荒木 元朗, 渡部 昌実, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 4 )   181 - 181   2018年4月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    J-GLOBAL

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  • 非セミノーマにおいて好中球・リンパ球比(NLR)は予後予測因子となる

    丸山 雄樹, 荒木 元朗, 光井 洋介, 定平 卓也, 西村 慎吾, 高本 篤, 和田 耕一郎, 谷本 竜太, 杉本 盛人, 小林 泰之, 渡邊 豊彦, 那須 保友

    日本泌尿器科学会総会   106回   OP - 041   2018年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

    J-GLOBAL

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  • 上部尿路上皮癌の診断法の再考 上部尿路上皮癌の診断における尿管鏡の有用性の再考

    荒木 元朗, 和田 耕一郎, 谷本 竜太, 佐々木 克己, 高本 篤, 杉本 盛人, 小林 泰之, 渡辺 豊彦, 那須 保友

    日本泌尿器科学会総会   106回   SY9 - 2   2018年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

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  • 非セミノーマにおいて好中球・リンパ球比(NLR)は予後予測因子となる

    丸山 雄樹, 荒木 元朗, 光井 洋介, 定平 卓也, 西村 慎吾, 高本 篤, 和田 耕一郎, 谷本 竜太, 杉本 盛人, 小林 泰之, 渡邊 豊彦, 那須 保友

    日本泌尿器科学会総会   106回   OP - 041   2018年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

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  • 体腔鏡下膀胱全摘・尿路変向のpitfall 岡山大学病院における腹腔鏡下膀胱全摘除術の経験

    小林 泰之, 杉本 盛人, 高本 篤, 和田 耕一郎, 荒木 元朗, 渡辺 豊彦, 那須 保友

    日本泌尿器科学会総会   106回   SY15 - 3   2018年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

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  • 精巣腫瘍に対する化学療法後の大腰筋体積の変化は新しい予後予測因子

    光井 洋介, 丸山 雄樹, 定平 卓也, 西村 慎吾, 高本 篤, 和田 耕一郎, 杉本 盛人, 荒木 元朗, 小林 泰之, 渡辺 豊彦, 那須 保友

    日本泌尿器科学会総会   106回   OP - 042   2018年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

    J-GLOBAL

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  • 筋層非浸潤性膀胱癌に対する経尿道的膀胱腫瘍切除術後のピラルビシン単回注入と2回注入の無作為化比較試験

    岩田 健宏, 谷本 竜太, 公文 裕巳, 大岩 裕子, 西村 慎吾, 高本 篤, 杉本 盛人, 和田 耕一郎, 小林 泰之, 荒木 元朗, 渡邉 豊彦, 那須 保友, 江原 伸, 雑賀 隆史, 那須 良次, 山田 大介, 高本 均, 宮地 禎幸, 津島 知靖, 佐々木 克己, 小野 憲昭

    西日本泌尿器科   80 ( 4 )   183 - 183   2018年4月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 超高齢時代の筋層浸潤性膀胱癌に対する低侵襲手術 岡山大学における高齢者に対する腹腔鏡下膀胱全摘除術の経験

    小林 泰之, 大岩 裕子, 高本 篤, 和田 耕一郎, 杉本 盛人, 荒木 元朗, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 4 )   132 - 136   2018年4月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

    岡山大学病院にて膀胱全摘除術を施行した81例(腹腔鏡下膀胱全摘除術(LRC)46例、開腹膀胱全摘除術(ORC)35例)を対象に、75歳以上を高齢者と定義し、75歳以上と75歳未満に層別し解析を行った。75歳以上の平均出血量(LRC 530ml、ORC 1,165ml:p=0.003)と術後14日目のヘモグロビン値(LRC 10.7g/dl、ORC 9.5g/dl:p=0.006)が統計学的に有意な差をもってLRCが優れた結果であった。平均入院日数は、75歳以上のLRC 30.4日、ORC 32.4日とLRCの方がやや短く、術後の平均歩行開始日はLRC 1.4日、ORC 2.3日(p=0.03)と統計学的に有意な差をもってLRCが早かった。術後の合併症(all grades)に関しては、75歳以上のLRC 50%、ORC 59%で、75歳未満のLRC 15.9%、ORC 33.3%に比べると高率に生じていた。今回の検討では、高齢者におけるLRCの低侵襲性が示され、また、LRCでは高齢者でも75歳未満の症例と同様の回復(術後ヘモグロビン値、歩行開始日)であった。しかしながら、高齢者ではLRCにおいても高率に術後合併症が発生しており、その適応は慎重に判断すべきである。(著者抄録)

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  • 精巣腫瘍に対する化学療法後の大腰筋体積の変化は新しい予後予測因子

    光井 洋介, 丸山 雄樹, 定平 卓也, 西村 慎吾, 高本 篤, 和田 耕一郎, 杉本 盛人, 荒木 元朗, 小林 泰之, 渡辺 豊彦, 那須 保友

    日本泌尿器科学会総会   106回   OP - 042   2018年4月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器科学会総会事務局  

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  • 他科から泌尿器科にコンサルトすべき尿路・性器感染症-治療すべき症例選択- カテーテル関連尿路感染症の臨床的意義

    和田 耕一郎, 定平 卓也, 山本 満寿美, 光畑 律子, 石井 亜矢乃, 渡邉 豊彦, 那須 保友

    感染症学雑誌   92 ( 2 )   168 - 168   2018年3月

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    記述言語:日本語   出版者・発行元:(一社)日本感染症学会  

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  • 高リスク前立腺癌に対するロボット支援腹腔鏡下前立腺全摘除術の摘出病理に関する検討

    高本 篤, 谷本 竜太, 小林 泰之, 和田里 章悟, 河村 香澄, 小林 宏州, 丸山 雄樹, 土井 啓介, 光井 洋介, 前原 貴典, 大岩 裕子, 定平 卓也, 岩田 健宏, 西村 慎吾, 和田 耕一郎, 杉本 盛人, 荒木 元朗, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 3 )   118 - 118   2018年3月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 他科から泌尿器科にコンサルトすべき尿路・性器感染症:治療すべき症例選択 カテーテル関連尿路感染症の臨床的意義

    和田 耕一郎, 定平 卓也, 山本 満寿美, 光畑 律子, 石井 亜矢乃, 渡邉 豊彦, 那須 保友

    日本化学療法学会雑誌   66 ( 2 )   224 - 224   2018年3月

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    記述言語:日本語   出版者・発行元:(公社)日本化学療法学会  

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  • 高位精巣摘除術14年後に再発を認めたStageIセミノーマの1例

    和田里 章悟, 高本 篤, 丸山 雄樹, 河村 香澄, 小林 宏州, 土井 啓介, 光井 洋介, 前原 貴典, 大岩 裕子, 定平 卓也, 岩田 健宏, 西村 慎吾, 堀川 雄平, 甲斐 誠二, 杉本 盛人, 谷本 竜太, 和田 耕一郎, 小林 泰之, 荒木 元朗, 渡邉 豊彦, 那須 保友

    西日本泌尿器科   80 ( 3 )   117 - 117   2018年3月

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    記述言語:日本語   出版者・発行元:西日本泌尿器科学会  

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  • 特集:泌尿器科診療におけるゲノム医療の可能性

    那須保友

    Urology Today   25 ( 1 )   22 - 38   2018年3月

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    記述言語:日本語  

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  • 肝腎同時移植における腎保護

    荒木元朗, 和田耕一郎, 山下里美, 丸山雄樹, 光井洋介, 定平卓也, 西村慎吾, 甲斐誠二, 高本篤, 谷本竜太, 杉本盛人, 小林泰之, 渡邉豊彦, 那須保友, 北川正史, 田邊克幸, 和田淳, 楳田祐三, 藤原俊義, 八木孝仁

    日本臨床腎移植学会プログラム・抄録集   51st   97   2018年

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    J-GLOBAL

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  • 第23回日本遺伝子細胞治療学会学術集会(JSGCT2017岡山)を終えて

    那須保友

    岡山医学会雑誌   129 ( 3 )   203 - 205   2017年12月

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    記述言語:日本語  

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  • 第23回日本遺伝子細胞治療学会学術集会(2017年7月20~22日,岡山)

    那須保友

    Urology Today   24 ( 4 )   54 - 55   2017年12月

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    記述言語:日本語  

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  • 高リスク前立腺癌に対するロボット支援腹腔鏡下前立腺全摘除術の摘出病理に関する検討

    高本 篤, 谷本 竜太, 小林 泰之, 西村 慎吾, 甲斐 誠司, 和田 耕一郎, 杉本 盛人, 荒木 元朗, 渡邉 豊彦, 那須 保友

    Japanese Journal of Endourology   30 ( 3 )   207 - 207   2017年11月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器内視鏡学会  

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  • 上部尿路腫瘍に対する腎盂尿管鏡による診断と治療 概論

    荒木 元朗, 和田 耕一郎, 谷本 竜太, 西村 慎吾, 高本 篤, 甲斐 誠二, 杉本 盛人, 小林 泰之, 渡辺 豊彦, 那須 保友

    Japanese Journal of Endourology   30 ( 3 )   141 - 141   2017年11月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器内視鏡学会  

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  • HDIG(High Definition Image Guide)スコープを用いた直視下腎杯穿刺とPCNLの検討

    和田 耕一郎, 公文 裕巳, 谷本 竜太, 佐々木 克己, 岩田 健宏, 高本 篤, 荒木 元朗, 小林 泰之, 渡邉 豊彦, 那須 保友

    Japanese Journal of Endourology   30 ( 3 )   187 - 187   2017年11月

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    記述言語:日本語   出版者・発行元:(一社)日本泌尿器内視鏡学会  

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  • 岡山大学病院におけるロボット支援腹腔鏡下腎部分切除術の検討

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