Updated on 2024/04/20

写真a

 
MARUYAMA Takayuki
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Associate Professor
Position
Associate Professor
External link

Degree

  • 博士(歯学) ( 2012.3   岡山大学 )

  • Ph.D. ( 2012.3   Okayama University )

Research Interests

  • 周術期口腔機能管理

  • 予防歯科学

  • 頭頸部がん

  • 歯周病

  • 動物実験

  • 臨床研究

  • 疫学研究

Research Areas

  • Life Science / Social dentistry

Education

  • Okayama University   大学院医歯薬学総合研究科   口腔保健学分野

    2007.4 - 2012.3

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  • Okayama University   歯学部   歯学科

    2001.4 - 2007.3

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Research History

  • 岡山大学学術研究院医歯薬学域   准教授

    2023.12

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  • 岡山大学学術研究院医歯薬学域   助教

    2020.4 - 2023.11

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  • Okayama University   新医療研究開発センター   Assistant Professor

    2014.1 - 2020.3

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  • Okayama University   Graduate School of Medicine , Dentistry and Pharmaceutical Sciences   Assistant Professor

    2012.4 - 2013.12

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Committee Memberships

  • 日本口腔衛生学会   査読委員  

    2021.5   

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    Committee type:Academic society

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  • 岡山大学臨床研究審査委員会   副委員長  

    2020.4   

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    Committee type:Other

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  • 日本口腔衛生学会   代議員  

    2013.5   

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    Committee type:Academic society

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Papers

  • サルコペニアと口腔機能との関連(横断研究)

    稲田 さくら, 竹内 倫子, 澤田 ななみ, 外山 直樹, 横井 彩, 丸山 貴之, 角田 伊知郎, 福原 大樹, 森田 学, 江國 大輔

    口腔衛生学会雑誌   74 ( 1 )   60 - 60   2024.1

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    Language:Japanese   Publisher:(一社)日本口腔衛生学会  

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  • Effects of Bacillus subtilis on periodontitis in a mouse model(タイトル和訳中)

    Zhang Yixuan, Mohammad Nurhamim, 中原 桃子, 福原 大樹, 丸山 貴之, 江國 大輔

    岡山歯学会雑誌   42 ( 2 )   76 - 76   2023.12

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    Language:English   Publisher:岡山歯学会  

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  • 口腔支持療法により外来放射線治療を完遂できた口腔癌3症例

    武田 斉子, 有村 友紀, 田村 庄平, 中辻 和樹, 三上 彩可, 松崎 秀信, 丸山 貴之, 横井 彩, 天野 克比古, 水川 展吉, 飯田 征二

    日本口腔ケア学会雑誌   17 ( 3 )   242 - 242   2023.4

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    Language:Japanese   Publisher:(一社)日本口腔ケア学会  

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  • 大学生における歯周病発症と受動喫煙を含む危険因子との関係 コホート研究

    内田 悠理香, 江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, 福原 瑶子, 外山 直樹, 澤田 ななみ, 中島 千穂, 中原 桃子, 角田 伊知郎, 森田 学

    口腔衛生学会雑誌   73 ( 増刊 )   149 - 149   2023.4

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  • 大学生における主観的口腔健康評価の傾向と,口腔健康状態および口腔保健行動との関連性

    中原 桃子, 外山 直樹, 江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, 澤田 ななみ, 中島 千穂, 森田 学

    岡山歯学会雑誌   41 ( 2 )   61 - 61   2022.12

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    Language:Japanese   Publisher:岡山歯学会  

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  • The Impact of Oral Health Behaviors, Health Belief Model, and Absolute Risk Aversion on the Willingness of Japanese University Students to Undergo Regular Dental Check-Ups: A Cross-Sectional Study Reviewed International journal

    Ichiro Sumita, Naoki Toyama, Daisuke Ekuni, Takayuki Maruyama, Aya Yokoi, Daiki Fukuhara, Yoko Uchida-Fukuhara, Momoko Nakahara, Manabu Morita

    International Journal of Environmental Research and Public Health   19 ( 21 )   13920 - 13920   2022.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Oral health behaviors, risk aversion, and the health belief model are associated with health behaviors. However, there have been few studies that investigated the association between these factors and the willingness to undergo regular dental check-ups. The purpose of this cross-sectional study was to investigate the associations between the willingness of Japanese university students to undergo regular dental check-ups and oral health behaviors, the health belief model, and absolute risk aversion. An analysis was conducted with the cooperation of questionnaire respondents (n = 748) who underwent dental check-ups at Okayama University. The students answered questionnaires on oral health behaviors, the health belief model, absolute risk aversion, and willingness to undergo regular dental check-ups. The logistic regression analysis showed significant positive associations (p < 0.05) between oral health behaviors (use of the inter-dental brush and the dental floss) and the health belief model with the willingness to undergo regular dental check-ups. However, there was no significant association with absolute risk aversion (p > 0.05). These results suggest that willingness to undergo regular dental check-ups was associated with oral health behaviors and the health belief model, but not with absolute risk aversion.

    DOI: 10.3390/ijerph192113920

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  • Trends in Self-Rated Oral Health and Its Associations with Oral Health Status and Oral Health Behaviors in Japanese University Students: A Cross-Sectional Study from 2011 to 2019. Reviewed International journal

    Momoko Nakahara, Naoki Toyama, Daisuke Ekuni, Noriko Takeuchi, Takayuki Maruyama, Aya Yokoi, Daiki Fukuhara, Nanami Sawada, Yukiho Nakashima, Manabu Morita

    International journal of environmental research and public health   19 ( 20 )   2022.10

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    Self-rated oral health (SROH) is a valid, comprehensive indicator of oral health status. The purpose of this cross-sectional study was to analyze how oral health behaviors and clinical oral status were associated with SROH and how they had changed over the course of nine years in Japanese university students. Data were obtained from 17,996 students who underwent oral examinations and completed self-questionnaires from 2011 to 2019. Oral status was assessed using the decayed and filled teeth scores, bleeding on probing (BOP), probing pocket depth, the Oral Hygiene Index-Simplified (OHI-S), oral health behaviors, and related factors. SROH improved from 2011 to 2019. The logistic regression model showed that university students who were female and had a high daily frequency of tooth brushing, no BOP, no decayed teeth, no filled teeth, and a low OHI-S score and were significantly more likely to report very good, good, or fair SROH. An interaction effect was observed between survey year and regular dental check-ups (year × regular dental check-ups). The improvement trend in SROH might be associated with changes in oral health behaviors and oral health status.

    DOI: 10.3390/ijerph192013580

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  • Relationship between Psychological Stress Determined by Voice Analysis and Periodontal Status: A Cohort Study. Reviewed International journal

    Takayuki Maruyama, Daisuke Ekuni, Masakazu Higuchi, Eiji Takayama, Shinichi Tokuno, Manabu Morita

    International journal of environmental research and public health   19 ( 15 )   2022.8

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    In modern society, evaluation and management of psychological stress may be important for the prevention of periodontal disease. The purpose of this study was to examine the relationship between psychological stress (vitality and mental activity) evaluated by Mind Monitoring System (MIMOSYS) and periodontal status. Forty students of Okayama University underwent the oral examination and self-reported questionnaire on the first day (baseline) and the 14th day (follow-up). Voice recording was performed every day with the MIMOSYS app during the whole study period. The participants completed the Patient Health Questionnaire (PHQ)-9 and Beck Depression Inventory (BDI) at baseline and at follow-up. Spearman's rank correlation coefficient was used to determine the significance of correlations among variables. The PHQ-9 and BDI scores were negatively correlated with vitality in the morning. Change in vitality in the morning was significantly correlated with changes in periodontal inflammation. Mental activity was significantly correlated with change in mean probing pocket depth. This result shows that measurement of psychological stress using a voice-based tool to assess mental health may contribute to the early detection of periodontal disease.

    DOI: 10.3390/ijerph19159489

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  • Association between serum miRNAs and gingival gene expression in an obese rat model Reviewed

    Takayuki Maruyama, Terumasa Kobayashi, Yoshio Sugiura, Toshiki Yoneda, Daisuke Ekuni, Manabu Morita

    Journal of Periodontal Research   57 ( 3 )   502 - 509   2022.6

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    Introduction: Recent studies have reported a relationship between periodontitis and obesity; however, the mechanisms of obesity’s effects on periodontitis are not well understood. On the other hand, microRNAs (miRNAs) are known to play key roles in the post-transcriptional regulation gene expression by suppressing translation and protein synthesis. We examined the association between obesity-related miRNAs and gene expression in gingival tissue using miRNA–messenger RNA (mRNA) pairing analysis in an obese rat model. Methods: Sixteen male Wistar rats aged 8 weeks old were divided into two groups: the control group was fed a normal powdered food for 8 weeks, and the obesity group was fed a high-fat diet for 8 weeks. Distance from the cement–enamel junction to the alveolar bone crest of the first molars was measured. miRNA microarray analysis was performed on samples of serum and gingival tissue; the resulting data were used to calculate fold changes in miRNA levels in the obesity group relative to the control group, and miRNA–mRNA pairing analysis was performed to identify mRNAs potentially targeted by miRNAs of interest. Results: Alveolar bone loss in the obesity group exceeded that in the control group (p =.017). miRNA–mRNA pairing analysis identified an association between 4 miRNAs (miR-759, miR-9a-3p, miR-203b-3p, and miR-878) that were differentially expressed in the obesity and control groups and 7 genes (Ly86, Arid5b, Rgs18, Mlana, P2ry13, Kif1b, and Myt1) expressed in gingival tissue. Conclusion: This study revealed that several miRNAs play an important role in the mechanism of periodontal disease progression induced by the obesity.

    DOI: 10.1111/jre.12979

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  • コロナ禍前とコロナ禍における大学生の睡眠時ブラキシズムの検討(横断研究)

    江國 大輔, 丸山 貴之, 横井 彩, 福原 大樹, 外山 直樹, 澤田 ななみ, 中原 桃子, 角田 伊知郎, 森田 学

    口腔衛生学会雑誌   72 ( 増刊 )   79 - 79   2022.4

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  • 周術期のガム咀嚼トレーニングは胸部食道がん患者の咀嚼能力を維持・向上させる

    山中 玲子, 白川 靖博, 横井 彩, 中村 吉秀, 難波 奏人, 田辺 俊介, 野間 和広, 清水 一好, 内田 悠理香, 吉冨 愛子, 丸山 貴之, 江國 大輔, 森田 学

    口腔衛生学会雑誌   72 ( 増刊 )   101 - 101   2022.4

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  • 舌背への光線力学療法による口臭の改善効果の検討 ランダム化比較試験

    長崎 潤一郎, 丸山 貴之, 横井 彩, 江國 大輔, 澤田 ななみ, 森田 学

    口腔衛生学会雑誌   72 ( 増刊 )   81 - 81   2022.4

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  • 大学病院における口腔ケア 岡山大学病院頭頸部がんセンターにおける顎骨再建後のインプラントと口腔ケアの重要性

    水川 展吉, 武田 斉子, 木股 敬裕, 松本 洋, 安藤 瑞生, 丸山 貴之, 佐々木 禎子, 田中 祐貴, 上田 明広, 飯田 征二

    日本口腔ケア学会雑誌   16 ( 3 )   83 - 83   2022.4

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  • 家庭での毎日の受動喫煙曝露は大学生の歯周病と関係する(横断研究)

    江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, 外山 直樹, イスラム・モハマド・モニルル, 澤田 ななみ, 中原 桃子, 中島 千穂, 出口 純也, 福原 瑶子, 森田 学

    口腔衛生学会雑誌   72 ( 1 )   54 - 54   2022.1

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  • 岡山大学新入生の口腔健康状態および主観的口腔健康評価の9年間の変化

    中原 桃子, 外山 直樹, 江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, イスラム・モハマド・モニルル, 澤田 ななみ, 中島 千穂, 出口 純也, 森田 学

    口腔衛生学会雑誌   72 ( 1 )   54 - 54   2022.1

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  • Detection of Salivary miRNAs That Predict Chronic Periodontitis Progression: A Cohort Study. Reviewed International journal

    Kohei Fujimori, Toshiki Yoneda, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Yoshio Sugiura, Manabu Morita

    International journal of environmental research and public health   18 ( 15 )   2021.7

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    The aim of this two-year cohort study was to investigate salivary microRNAs (miRNAs) that predict periodontitis progression. A total of 120 patients who underwent supportive periodontal therapy were recruited. Unstimulated whole saliva was collected at baseline. Two years later, 44 patients were followed up (median age, 67.1 years) and divided into two groups: progression group (n = 22), with one or more sites with clinical attachment level (CAL) progression (>3 mm compared with baseline) or tooth extraction due to periodontitis progression; and the control group (n = 22), which did not exhibit CAL progression. In the microarray analysis of salivary miRNAs, hsa-miR-5571-5p, hsa-miR-17-3p, hsa-let-7f-5p, hsa-miR-4724-3p, hsa-miR-99a-5p, hsa-miR-200a-3p, hsa-miR-28-5p, hsa-miR-320d, and hsa-miR-31-5p showed fold change values <0.5 or ≥2.0 in the progression group compared with the control group (p < 0.05). On receiver operating characteristic curve analysis, areas under the curves of hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were >0.7, indicating fair discrimination power. The expressions of salivary hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were associated with periodontitis progression in patients with chronic periodontitis. These salivary miRNAs may be new biomarkers for progression of periodontitis, and monitoring them may contribute to new diagnostics and precision medicine for periodontitis.

    DOI: 10.3390/ijerph18158010

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  • Parafunctional masseter muscle activity during waking is related to periodontitis progression: A pilot prospective cohort study. Reviewed International journal

    Daisuke Ekuni, Seiya Kato, Shigehisa Kawakami, Takayuki Maruyama, Kota Kataoka, Yoko Uchida-Fukuhara, Daiki Fukuhara, Naoki Toyama, Aya Yokoi, Md Monirul Islam, Subrina Binta Khair, Naoki Kodama, Manabu Morita

    Journal of clinical periodontology   48 ( 6 )   785 - 794   2021.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    AIM: The purpose of this pilot prospective cohort study was to investigate the effects of parafunctional masseter muscle activity on periodontitis progression among patients receiving supporting periodontal therapy (SPT). MATERIALS AND METHODS: We collected data of patients treated at Okayama University Hospital from August 2014 to September 2018. The progression group was defined as patients with ≥2 teeth demonstrating a longitudinal loss of proximal attachment of ≥3 mm during the 3-year study period and/or at least one tooth extraction due to periodontitis progression. Surface electromyography of masseter muscles at baseline was continuously recorded while patients were awake and asleep. RESULTS: We analysed 48 patients (36 females) aged 66.8 ± 9.1 years (mean ± SD). The rate of parafunctional masseter muscle activity during waking hours and sleeping hours at baseline was 60.4% and 52.1%, respectively. Cox's proportional hazards regression model showed that the incidence of periodontitis progression was significantly associated with number of teeth present (p = 0.001) and parafunctional masseter muscle activity during waking hours (p = 0.041). CONCLUSION: Our results suggest that parafunctional masseter muscle activity during waking hours is a risk factor for periodontitis progression among patients receiving SPT.

    DOI: 10.1111/jcpe.13432

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jcpe.13432

  • 噛みたばこ習慣のあるミャンマーでの口腔がん検診

    水川 展吉, Swe Swe Win, 加藤 昌, 丸山 貴之, 山近 英樹, 木股 敬裕, 飯田 征二

    頭頸部癌   47 ( 2 )   244 - 244   2021.5

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  • 「口臭による日常生活への支障」に関連する要因

    佐保 輝, 横井 彩, 丸山 貴之, 竹内 倫子, 江國 大輔, 森田 学

    日本口臭学会会誌   12 ( 1 )   11 - 17   2021.5

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    Language:Japanese   Publisher:日本口臭学会  

    本調査の目的は、自己記入式質問票の「社会生活への支障」「家庭生活への支障」「行動制限」「自身の口臭が原因による他人の嫌がる態度やジェスチャー」の4項目を「口臭による日常生活への支障」と定義しその実態を把握すること、および、「口臭による日常生活への支障」と関連する要因について検討することである。2018年1月から2020年1月までの間に岡山大学病院口臭専門外来を受診した全患者32名(男性12名、女性20名、平均年齢53.2±16.2歳)を対象とした。オーラルクロマ(NISSHAエフアイエス株式会社、大阪)を用い、口腔内の揮発性硫黄化合物の濃度を測定した。自己記入式の質問票を用いて、上記4項目のほかに年齢、性別、口臭を意識するようになったきっかけ、口臭を意識した時期、頻回の歯磨き・うがい薬の使用・舌磨きによる口臭対策、医療者への相談経験を調査した。なお、口臭による日常生活への支障については、「社会生活への支障」「家庭生活への支障」「行動制限」「自身の口臭が原因による他人の嫌がる態度やジェスチャー」の各質問に対する回答【はい(1点)/いいえ(0点)】をもとに、合計スコア0〜4点で評価した。各質問項目との関連についてSpearmanの順位相関係数とカイ二重検定を用いて検討した。有意水準は5%とした。その結果、本調査で定義した「口臭による日常生活への支障」を有する患者が87.5%存在した。その支障の程度が大きい者は、長期にわたって口臭を意識しており、医療者への相談や複数の口臭対策を行っていた。一方で、実際の口腔内の揮発性硫黄化合物の濃度は有意に低いことが明らかになった。本調査結果は、口臭専門外来における新たな視点を提供する可能性がある。(著者抄録)

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  • 「口臭による日常生活への支障」に関連する要因

    佐保 輝, 横井 彩, 丸山 貴之, 竹内 倫子, 江國 大輔, 森田 学

    日本口臭学会会誌   12 ( 1 )   49 - 50   2021.5

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  • 岡山大学病院頭頸部がんセンターにおける頭頸部がん支持療法に関する臨床実習教育の取り組みについて

    丸山 貴之, 横井 彩, 中原 桃子, 佐々木 禎子, 梶谷 明子, 藤代 万由, 中本 美保, 吉田 陽子, 森田 学, 三浦 留美, 山中 玲子, 水川 展吉, 浅海 淳一, 安藤 瑞生, 木股 敬裕

    日本口腔ケア学会雑誌   15 ( 3 )   186 - 186   2021.4

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  • ヘルスリテラシーとリスク選好が定期的な歯科健診への受診意志に与える影響

    角田 伊知郎, 森田 学, 江國 大輔, 丸山 貴之, 片岡 広太, 横井 彩, 福原 大樹, 福原 瑶子, 外山 直樹, 中原 桃子

    口腔衛生学会雑誌   71 ( 増刊 )   88 - 88   2021.4

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  • 頭頸部がん治療による口腔機能・QOLへの影響 縦断研究

    横井 彩, 丸山 貴之, 中原 桃子, 山中 玲子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   71 ( 増刊 )   116 - 116   2021.4

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  • 岡山大学病院頭頸部がんセンターにおけるエピシル使用経験について

    武田 斉子, 水川 展吉, 田村 庄平, 岡田 亜由美, 中田 靖章, 横井 彩, 丸山 貴之, 松崎 秀信, 松崎 久美子, 藤代 万由, 佐々木 禎子, 山中 玲子, 安藤 瑞生, 木股 敬裕, 浅海 淳一, 飯田 征二

    日本口腔ケア学会雑誌   15 ( 3 )   172 - 172   2021.4

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  • Effects of Coffee Intake on Oxidative Stress During Aging-related Alterations in Periodontal Tissue Reviewed

    TERUMASA KOBAYASHI, TAKAYUKI MARUYAMA, TOSHIKI YONEDA, HISATAKA MIYAI, TETSUJI AZUMA, TAKAAKI TOMOFUJI, DAISUKE EKUNI, MANABU MORITA

    In Vivo   34 ( 2 )   615 - 622   2020.3

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    DOI: 10.21873/invivo.11815

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  • 慢性歯周炎患者の歯周状態の悪化に関連する唾液中miRNAの探索 コホート研究

    藤森 浩平, 米田 俊樹, 杉浦 嘉雄, 丸山 貴之, 江國 大輔, 東 哲司, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   70 ( 増刊 )   158 - 158   2020.3

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  • Detection of Serum miRNAs Affecting Liver Apoptosis in a Periodontitis Rat Model Reviewed

    YOSHIO SUGIURA, TOSHIKI YONEDA, KOHEI FUJIMORI, TAKAYUKI MARUYAMA, HISATAKA MIYAI, TERUMASA KOBAYASHI, DAISUKE EKUNI, TAKAAKI TOMOFUJI, MANABU MORITA

    In Vivo   34 ( 1 )   117 - 123   2020.1

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  • Relationship between acetaldehyde concentration in mouth air and characteristics of microbiota of tongue dorsum in Japanese healthy adults: a cross-sectional study. Reviewed International journal

    Aya Yokoi, Daisuke Ekuni, Hironobu Hata, Mayu Yamane-Takeuchi, Takayuki Maruyama, Reiko Yamanaka, Manabu Morita

    Journal of applied oral science : revista FOB   27   e20180635   2019.6

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    OBJECTIVE: Acetaldehyde, associated with consumption of alcoholic beverages, is known to be a carcinogen and to be related to the tongue dorsum. The aim of this study was to investigate the relationship between acetaldehyde concentration in mouth air and bacterial characteristics on the tongue dorsum. METHODOLOGY: Thirty-nine healthy volunteers participated in the study. Acetaldehyde concentrations in mouth air were evaluated by a high-sensitivity semiconductor gas sensor. A 16S rRNA gene sequencing technique was used to compare microbiomes between two groups, focusing on the six samples with the highest acetaldehyde concentrations (HG) and the six samples with lowest acetaldehyde concentrations (LG). RESULTS: Acetaldehyde concentration increased in correlation with the increase in bacterial count (p=0.048). The number of species observed in the oral microbiome of the HG was higher than that in the oral microbiome of the LG (p=0.011). The relative abundances of Gemella sanguinis, Veillonella parvula and Neisseria flavescens in the oral microbiome of the HG were higher than those in the oral microbiome of the LG (p<0.05). CONCLUSION: Acetaldehyde concentration in mouth air was associated with bacterial count, diversity of microbiome, and relative abundance of G. sanguinis, V. parvula, and N. flavescens.

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  • Serum microRNAs and chronic periodontitis: A case-control study. Reviewed International journal

    Toshiki Yoneda, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Kohei Fujimori, Yoshio Sugiura, Manabu Morita

    Archives of oral biology   101   57 - 63   2019.5

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    OBJECTIVE: An association is present between periodontitis and rates of expression of certain microRNAs (miRNAs) in periodontal tissue. However, the association between periodontitis and miRNA levels in human serum is unknown. We performed a case-control study in patients with chronic periodontitis to investigate serum miRNA levels. DESIGN: We enrolled 30 healthy patients without periodontitis and 30 patients with chronic periodontitis. Participants underwent clinical examination, case selection, and a blood draw from the antecubital vein. Serum miRNA profiles were compared in samples from participants with and without chronic periodontitis using microarray and real-time PCR. RESULTS: Microarray demonstrated seven miRNAs that were expressed <1/1.5 or >1.5 in the control group compared to the periodontitis group (p < 0.05). Real-time PCR showed that hsa-miR-664a-3p, hsa-miR-501-5p, and hsa-miR-21-3p were higher in the periodontitis group than the control group (p < 0.05). CONCLUSION: Hsa-miR-664a-3p, hsa-miR-501-5p, and hsa-miR-21-3p are candidate serum biomarkers for chronic periodontitis.

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  • Detection of Salivary miRNAs Reflecting Chronic Periodontitis: A Pilot Study. Reviewed International journal

    Kohei Fujimori, Toshiki Yoneda, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Hirofumi Mizuno, Yoshio Sugiura, Manabu Morita

    Molecules (Basel, Switzerland)   24 ( 6 )   2019.3

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    The purpose of this cross-sectional pilot study was to find salivary microRNAs (miRNAs) reflecting periodontal condition in chronic periodontitis. One hundred and twenty chronic periodontitis patients (mean age, 68.4 years) participated in the study, from whom unstimulated whole saliva was collected. A multiphase study was conducted to explore salivary miRNAs as biomarkers of periodontitis. At first, a polymerase chain reaction (PCR) array was performed to compare salivary miRNAs profiles in no and mild (no/mild) and severe periodontitis patients. Next, the relative expression of salivary miRNAs on individual samples was assessed by real-time reverse transcription-PCR. The numbers (%) of patients were 26 (21.6%, no/mild), 58 (48.3%, moderate) and 36 (30.0%, severe), respectively. Among 84 miRNAs, only the relative expression of hsa-miR-381-3p in the severe periodontitis group was significantly higher than that of the no/mild periodontitis group (p < 0.05). Among the 120 patients, there was also a significant correlation between the relative expression of hsa-miR-381-3p and the mean probing pocket depth (PPD) (r = 0.181, p < 0.05). Salivary hsa-miR-381-3p was correlated with periodontitis condition in chronic periodontitis patients.

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  • Relationship Between Renal Dysfunction and Oral Mucositis in Patients Undergoing Concurrent Chemoradiotherapy for Pharyngeal Cancer: A Retrospective Cohort Study. Reviewed

    Mizuno H, Miyai H, Yokoi A, Kobayashi T, Inabu C, Maruyama T, Ekuni D, Mizukawa N, Kariya S, Nishizaki K, Kimata Y, Morita M

    In vivo (Athens, Greece)   33 ( 1 )   183 - 189   2019.1

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  • New oral hygiene care regimen reduces postoperative oral bacteria count and number of days with elevated fever in ICU patients with esophageal cancer. Reviewed

    Hirofumi Mizuno, Shinsuke Mizutani, Daisuke Ekuni, Ayano Tabata-Taniguchi, Takayuki Maruyama, Aya Yokoi, Chie Omori, Kazuyoshi Shimizu, Hiroshi Morimatsu, Yasuhiro Shirakawa, Manabu Morita

    Journal of oral science   60 ( 4 )   536 - 543   2018.12

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    Using a controlled pre/post study design, we investigated the effects of professional mechanical cleaning of the oral cavity with benzethonium chloride, interdental brushes, and hydrogen peroxide on the number of oral bacteria and postoperative complications among esophageal cancer patients in an intensive care unit. Before surgery, 44 patients with esophageal cancer were recruited at Okayama Hospital from January through August 2015. The control group (n = 23) received routine oral hygiene care in the intensive care unit. The intervention group (n = 21) received intensive interdental cleaning with benzethonium chloride solution and tongue cleaning with hydrogen peroxide. The number of oral bacteria on the tongue surface and plaque index were significantly lower in the intervention group than in the control group on postoperative days 1 and 2 (P < 0.05). Additionally, the number of days with elevated fever during a 1-week period was significantly lower in the intervention group than in the control group (P = 0.037). As compared with routine oral hygiene, a new oral hygiene regimen comprising benzethonium chloride, interdental brushes, and hydrogen peroxide significantly reduced the number of oral bacteria and days with elevated fever in patients with esophageal cancer.

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  • The effects of non-surgical periodontal treatment on glycemic control, oxidative stress balance and quality of life in patients with type 2 diabetes: A randomized clinical trial. Reviewed International journal

    Hirofumi Mizuno, Daisuke Ekuni, Takayuki Maruyama, Kota Kataoka, Toshiki Yoneda, Daiki Fukuhara, Yoshio Sugiura, Takaaki Tomofuji, Jun Wada, Manabu Morita

    PloS one   12 ( 11 )   e0188171   2017.11

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    Aim
    The purpose of this study was to investigate the effects of non-surgical periodontal treatment on hemoglobinA1c (HbA1c) levels, oxidative stress balance and quality of life (QOL) in patients with type 2 diabetes mellitus (T2DM) compared to no periodontal treatment (simple oral hygiene instructions only).
    Methods
    The design was a 6-month, single-masked, single center, randomized clinical trial. Patients had both T2DM and chronic periodontitis. Forty participants were enrolled between April 2014 and March 2016 at the Nephrology, Diabetology and Endocrinology Department of Okayama University Hospital. The periodontal treatment group (n = 20) received non-surgical periodontal therapy, including scaling and root planing plus oral hygiene instructions, and consecutive supportive periodontal therapy at 3 and 6 months. The control group (n = 17) received only oral hygiene instructions without treatment during the experimental period. The primary study outcome was the change in HbA1c levels from baseline to 3 months. Secondary outcomes included changes in oxidative stress balance (Oxidative-INDEX), the Diabetes Therapy-Related QOL and clinical periodontal parameters from baseline to 3 months and baseline to 6 months.
    Results
    Changes in HbA1c in the periodontal treatment group were not significantly different with those in the control group at 3 and 6 months. Systemic oxidative stress balance and QOL significantly improved in the periodontal treatment group compared to the control group at 3 months. In the subgroup analysis (moderately poor control of diabetes), the decrease in HbA1c levels in the periodontal treatment group was greater than that in the control group at 3 months but not significant.
    Conclusions
    In T2DM patients, non-surgical periodontal treatment improved systemic oxidative stress balance and QOL, but did not decrease HbA1c levels at 3 months follow-up.

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  • Topical application of ointment containing 0.5% green tea catechins suppresses tongue oxidative stress in 5-fluorouracil administered rats. Reviewed International journal

    Hisataka Miyai, Takayuki Maruyama, Takaaki Tomofuji, Toshiki Yoneda, Tetsuji Azuma, Hirofumi Mizuno, Yoshio Sugiura, Terumasa Kobayashi, Daisuke Ekuni, Manabu Morita

    Archives of oral biology   82   247 - 255   2017.10

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    Objective: The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats.
    Design: Male Wistar rats (n = 28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1% or 0.5% green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus.
    Results: After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P &lt; 0.05). On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group (P &lt; 0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5% green tea catechin group than in the positive control group (P &lt; 0.05).
    Conclusions: Topical application of ointment containing 0.5% green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.

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  • Expression of Salivary miR-203a-3p Was Related with Oral Health-Related Quality of Life in Healthy Volunteers. Reviewed International journal

    Terumasa Kobayashi, Takaaki Tomofuji, Tatsuya Machida, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Akiko Hirose, Manabu Morita

    International journal of molecular sciences   18 ( 6 )   2017.6

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    Oral health-related quality of life (OHRQoL) is a multidimensional construct that involves subjective evaluation of an individual's oral health. Although it is difficult to evaluate OHRQoL biologically, recently, it has been reported that circulating microRNAs (miRNAs) in several body fluids could reflect various health conditions. The aim of this pilot study was to investigate whether salivary miRNAs expression differs according to OHRQoL in healthy volunteers. Forty-six volunteers (median age, 23.0 years) were recruited, and their OHRQoL was assessed using the Japanese version of the Oral Health Impact Profile (OHIP-J). Then, we compared salivary microRNA profiles of the high-OHRQoL group (&lt;= 25th percentile score of OHIP-J) and the low-OHRQoL group (&gt;= 75th percentile score of OHIP-J) using the polymerase chain reaction (PCR) array and the quantitative real-time PCR. There were no significant differences between the two groups in terms of oral health status. In the PCR array, miR-203a-3p and miR-30b-5p were significantly more expressed in the low-OHRQoL group (p &lt; 0.05). Quantitative real-time PCR assay also showed that miR-203a-3p was more highly expressed in the low-OHRQoL group than in the high-OHRQoL group (p &lt; 0.05). These observations suggest that expression of salivary miR-203a-3p was related with OHRQoL in healthy volunteers.

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  • Association between periodontitis and prognosis of pancreatobiliary tract cancer: A pilot study. Reviewed International journal

    Takayuki Maruyama, Takaaki Tomofuji, Tatsuya Machida, Hironari Kato, Koichiro Tsutsumi, Daisuke Uchida, Akinobu Takaki, Toshiki Yoneda, Hisataka Miyai, Hirofumi Mizuno, Daisuke Ekuni, Hiroyuki Okada, Manabu Morita

    Molecular and clinical oncology   6 ( 5 )   683 - 687   2017.5

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    Several studies have indicated that periodontitis is a risk factor for cancer. However, the association between periodontitis and the prognosis of pancreatobiliary tract cancer remains unclear. The aim of this pilot study was to investigate the association between periodontitis and prognosis of pancreatobiliary tract cancer. A total of 22 patients diagnosed with pancreatobiliary tract cancer were analyzed. Oral health status, including severity of periodontitis, general health status and biochemical serum markers were evaluated. The Kaplan-Meier method and Cox proportional hazards model were used to assess factors affecting the prognosis of pancreatobiliary tract cancer. The Kaplan-Meier analysis demonstrated that low body mass index, high concentration of serum C-reactive protein (CRP) and severe periodontitis were significant prognostic factors for survival rate. The Cox proportional hazards model revealed that serum carbohydrate antigen 19-9 concentration [hazard ratio (HR)=1.002; 95% confidence interval (CI): 1.000-1.004] and serum CRP concentration (HR=2.57; 95% CI: 1.15-5.74) were significantly associated with the prognosis of pancreatobiliary tract cancer. In addition, cancer patients with severe periodontitis had higher serum CRP concentrations compared with those without severe periodontitis. Therefore, severe periodontitis indirectly affected the prognosis of pancreatobiliary tract cancer through promoting systemic inflammation.

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  • The Serum Oxidative/Anti-oxidative Stress Balance Becomes Dysregulated in Patients with Non-alcoholic Steatohepatitis Associated with Hepatocellular Carcinoma. Reviewed

    Yasuyuki Shimomura, Akinobu Takaki, Nozomu Wada, Tetsuya Yasunaka, Fusao Ikeda, Takayuki Maruyama, Naofumi Tamaki, Daisuke Uchida, Hideki Onishi, Kenji Kuwaki, Shinichiro Nakamura, Kazuhiro Nouso, Yasuhiro Miyake, Kazuko Koike, Takaaki Tomofuji, Manabu Morita, Kazuhide Yamamoto, Hiroyuki Okada

    Internal medicine (Tokyo, Japan)   56 ( 3 )   243 - 251   2017

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    Objective Oxidative stress is associated with the progression of chronic liver disease. Non-alcoholic fatty liver disease (NAFLD) is also an oxidative stress-related disease. However, the oxidative/anti-oxidative balance has not been fully characterized in NAFLD. The objective of the present study was to investigate the balance between oxidative stress and the anti-oxidative activity in NAFLD, including non-alcoholic steatohepatitis (NASH)-related hepatocellular carcinoma (HCC).
    Patients We recruited 69 patients with histologically proven NAFLD without HCC (NAFLD; n=58), and with NASH-related HCC (NASH-HCC; n=11). The 58 NAFLD patients included patients with non-alcoholic fatty liver (NAFL; n=14) and NASH (n=44).
    Methods The serum levels of reactive oxygen metabolites (ROM) and anti-oxidative markers (OXY) were determined and then used to calculate the oxidative index. The correlations among such factors as ROM, OXY, oxidative index, and clinical characteristics were investigated.
    Results In NAFLD, ROM positively correlated with the body mass index (BMI), hemoglobin A1c (HbA1c), C-reactive protein (CRP), and the histological grade or inflammatory scores, while only high HbA1c and CRP levels were significant factors that correlated with a higher ROM according to a multivariate analysis. OXY positively correlated with the platelet counts, albumin, and creatinine levels, while negatively correlating with age. However, it improved after treatment intervention. The oxidative index positively correlated with BMI, CRP, and HbA1c. The NASH-HCC patients exhibited a lower OXY than the NASH patients, probably due to the effects of aging.
    Conclusion Oxidative stress correlated with the levels of NASH activity markers, while the anti-oxidative function was preserved in younger patients as well as in patients with a well-preserved liver function. The NASH-HCC patients tended to be older and exhibited a diminished anti-oxidative function.

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  • miR‑1246 and miR‑4644 in salivary exosome as potential biomarkers for pancreatobiliary tract cancer. Reviewed International journal

    Tatsuya Machida, Takaaki Tomofuji, Takayuki Maruyama, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Hisataka Miyai, Hirofumi Mizuno, Hironari Kato, Koichiro Tsutsumi, Daisuke Uchida, Akinobu Takaki, Hiroyuki Okada, Manabu Morita

    Oncology reports   36 ( 4 )   2375 - 81   2016.10

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    Pancreatobiliary tract cancer is a highly fatal cancer. Detection of pancreatobiliary tract cancer is difficult because it lacks typical clinical symptoms and because of its anatomical location. Biomarker discovery is therefore important to detect pancreatobiliary tract cancer in its early stage. A study demonstrated that expression levels of miR-1246, miR-3976, miR-4306, and miR-4644 in serum exosomes were higher in pancreatic cancer patients than these levels in healthy control participants. Supposing that microRNA (miRNA) expression profiles in saliva are similar to those in serum, four miRNAs (miR-1246, miR-3976, miR-4306, and miR-4644) in salivary exosomes may also be useful for detection of pancreatobiliary tract cancer. In this study, it was examined whether these miRNAs could be used as biomarkers for pancreatobiliary tract cancer. Twelve pancreatobiliary tract cancer patients and 13 healthy control participants were analyzed as a cancer and a control group, respectively. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. Using quantitative real-time PCR (RT-qPCR), the relative expression ratios of miR-1246 and miR-4644 were significantly higher in the cancer group than these ratios in the control group. Receiver operating characteristic (ROC) curves were constructed to analyze the discrimination power of these miRNAs. For miR-1246, the results yielded an area under the curve (AUC) of 0.814 (P=0.008). For miR-4644, the results yielded an AUC of 0.763 (P=0.026). For the combination of miR-1246 and miR-4644, the results yielded an increased AUC of 0.833 (P=0.005). This pilot study suggests that miR-1246 and miR-4644 in salivary exosomes could be candidate biomarkers for pancreatobiliary tract cancer.

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  • Oxidative stress balance is dysregulated and represents an additional target for treating cholangiocarcinoma. Reviewed International journal

    Daisuke Uchida, Akinobu Takaki, Hisashi Ishikawa, Yasuko Tomono, Hironari Kato, Koichiro Tsutsumi, Naofumi Tamaki, Takayuki Maruyama, Takaaki Tomofuji, Ryuichiro Tsuzaki, Tetsuya Yasunaka, Kazuko Koike, Hiroshi Matsushita, Fusao Ikeda, Yasuhiro Miyake, Hidenori Shiraha, Kazuhiro Nouso, Ryuichi Yoshida, Yuzo Umeda, Susumu Shinoura, Takahito Yagi, Toshiyoshi Fujiwara, Manabu Morita, Masaki Fukushima, Kazuhide Yamamoto, Hiroyuki Okada

    Free radical research   50 ( 7 )   732 - 43   2016.7

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    Background: Pancreatico-biliary malignancies exhibit similar characteristics, including obesity-related features and poor prognosis, and require new treatment strategies. Oxidative stress is known to induce DNA damage and carcinogenesis, and its reduction is viewed as being favorable. However, it also has anti-infection and anti-cancer functions that need to be maintained. To reveal the effect of oxidative stress on cancer progression, we evaluated oxidative stress and anti-oxidative balance in pancreatic cancer (PC) and cholangiocarcinoma (CC) patients, as well as the effect of add-on antioxidant treatment to chemotherapy in a mouse cholangiocarcinoma model.
    Methods: We recruited 84 CC and 80 PC patients who were admitted to our hospital. Serum levels of reactive oxygen metabolites (ROM) and the anti-oxidative OXY-adsorbent test were determined and the balance of these tests was defined as an oxidative index. A diabetic mouse-based cholangiocarcinoma model was utilized to evaluate the effects of add-on antioxidant therapy on cholangiocarcinoma chemotherapy.
    Results: Serum ROM was higher and anti-oxidant OXY was lower in CC patients with poor outcomes. These parameters were not significantly different in PC patients. In mice, vitamin E administration induced antioxidant hemeoxygenase (HO)-1 protein expression in cancer tissue, while the number of stem-like cells increased. L-carnitine administration improved intestinal microbiome and biliary acid balance, upregulated the hepatic mitochondrial membrane uptake related gene Cpt1 in non-cancerous tissue, and did not alter stem-like cell numbers.
    Conclusion: Oxidative stress balance was dysregulated in cholangiocarcinoma with poor outcome. The mitochondrial function-supporting agent L-carnitine is a good candidate to control oxidative stress conditions.

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  • MicroRNAs as serum biomarkers for periodontitis. Reviewed International journal

    Takaaki Tomofuji, Toshiki Yoneda, Tatsuya Machida, Daisuke Ekuni, Tetsuji Azuma, Kota Kataoka, Takayuki Maruyama, Manabu Morita

    Journal of clinical periodontology   43 ( 5 )   418 - 25   2016.5

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    Aim: Studies demonstrated that periodontitis modulates microRNA (miRNAs) expression rates in periodontal tissue. However, the relationship between periodontitis and miRNAs profile in circulation remains unclear. In this study, we investigated the effects of periodontitis on serum miRNAs profile in a rat model.
    Material and Methods: Male Wistar rats (n=32, 8weeks old) were divided into four groups of eight rats each. The control groups received no treatment for 2 or 4weeks. In the other two groups, periodontitis was ligature induced for 2 or 4weeks. Serum miRNAs expression profiles of each group were compared.
    Results: Ligation around teeth induced periodontal inflammation at 2weeks and periodontal tissue destruction at 4weeks. Microarray results showed that 25 miRNAs were expressed with a &lt;0.5 or &gt;2 difference between the control and periodontitis groups at 4weeks. Results of real-time PCR revealed that the periodontitis group up-regulated expression rates of serum miR-207 and miR-495 at 2weeks, and miR-376b-3p at 4weeks (p&lt;0.05).
    Conclusion: Serum miRNAs (miR-207, miR-495, and miR-376b-3p) could be valuable biomarkers for periodontitis.

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  • MicroRNAs in Salivary Exosome as Potential Biomarkers of Aging. Reviewed International journal

    Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Takayuki Maruyama, Toshiki Yoneda, Yuya Kawabata, Hirofumi Mizuno, Hisataka Miyai, Muneyoshi Kunitomo, Manabu Morita

    International journal of molecular sciences   16 ( 9 )   21294 - 309   2015.9

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    The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.

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  • Drinking Hydrogen-Rich Water Has Additive Effects on Non-Surgical Periodontal Treatment of Improving Periodontitis: A Pilot Study. Reviewed International journal

    Tetsuji Azuma, Mayu Yamane, Daisuke Ekuni, Yuya Kawabata, Kota Kataoka, Kenta Kasuyama, Takayuki Maruyama, Takaaki Tomofuji, Manabu Morita

    Antioxidants (Basel, Switzerland)   4 ( 3 )   513 - 22   2015.7

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    Oxidative stress is involved in the pathogenesis of periodontitis. A reduction of oxidative stress by drinking hydrogen-rich water (HW) might be beneficial to periodontal health. In this pilot study, we compared the effects of non-surgical periodontal treatment with or without drinking HW on periodontitis. Thirteen patients (3 women, 10 men) with periodontitis were divided into two groups: The control group (n = 6) or the HW group (n = 7). In the HW group, participants consumed HW 4-5 times/day for eight weeks. At two to four weeks, all participants received non-surgical periodontal treatment. Oral examinations were performed at baseline, two, four and eight weeks, and serum was obtained at these time points to evaluate oxidative stress. At baseline, there were no significant differences in periodontal status between the control and HW groups. The HW group showed greater improvements in probing pocket depth and clinical attachment level than the control group at two, four and eight weeks (p < 0.05). The HW group also exhibited an increased serum level of total antioxidant capacity at four weeks, compared to baseline (p < 0.05). Drinking HW enhanced the effects of non-surgical periodontal treatment, thus improving periodontitis.

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  • Relationship between acetaldehyde concentration in mouth air and tongue coating volume Reviewed

    Aya Yokoi, Takayuki Maruyama, Reiko Yamanaka, Daisuke Ekuni, Takaaki Tomofuji, Haruhiko Kashiwazaki, Yutaka Yamazaki, Manabu Morita

    JOURNAL OF APPLIED ORAL SCIENCE   23 ( 1 )   64 - 70   2015.1

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    Objective: Acetaldehyde is the first metabolite of ethanol and is produced in the epithelium by mucosal ALDH, while higher levels are derived from microbial oxidation of ethanol by oral microflora such as Candida species. However, it is uncertain whether acetaldehyde concentration in human breath is related to oral condition or local production of acetaldehyde by oral microflora. The aim of this pilot study was to investigate the relationship between physiological acetaldehyde concentration and oral condition in healthy volunteers. Material and Methods: Sixty-five volunteers (51 males and 14 females, aged from 20 to 87 years old) participated in the present study. Acetaldehyde concentration in mouth air was measured using a portable monitor. Oral examination, detection of oral Candida species and assessment of alcohol sensitivity were performed. Results: Acetaldehyde concentration [median (25%, 75%)] in mouth air was 170.7 (73.5, 306.3) ppb. Acetaldehyde concentration in participants with a tongue coating status score of 3 was significantly higher than in those with a score of 1 (p&lt;0.017). After removing tongue coating, acetaldehyde concentration decreased significantly (p&lt;0.05). Acetaldehyde concentration was not correlated with other clinical parameters, presence of Candida species, smoking status or alcohol sensitivity. Conclusion: Physiological acetaldehyde concentration in mouth air was associated with tongue coating volume.

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  • Serum Oxidative/anti-oxidative Stress Balance Is Dysregulated in Potentially Pulmonary Hypertensive Patients with Liver Cirrhosis: A Case Control Study. Reviewed

    Masako Terao, Akinobu Takaki, Takayuki Maruyama, Hiroki Oe, Tetsuya Yasunaka, Naofumi Tamaki, Kazufumi Nakamura, Takaaki Tomofuji, Takahito Yagi, Hiroshi Sadamori, Yuzo Umeda, Susumu Shinoura, Ryuichi Yoshida, Kazuhiro Nouso, Daisuke Ekuni, Kazuko Koike, Fusao Ikeda, Hidenori Shiraha, Manabu Morita, Hiroshi Ito, Toshiyoshi Fujiwara, Kazuhide Yamamoto

    Internal medicine (Tokyo, Japan)   54 ( 22 )   2815 - 26   2015

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    Objective Hepatopulmonary syndrome (HPS) is characterized by vascular dilatation and hyperdynamic circulation, while portopulmonary hypertension (POPH) is characterized by vasoconstriction with fibrous obliteration of the vascular bed. Vasoactive molecules such as nitric oxide (NO) are candidate factors for cirrhotic complications associated with these diseases. However, oxidative stress balance is not well characterized in HPS and POPH. The present objective is to investigate the oxidative stress and anti-oxidative stress balance and NO pathway balance in patients with potential HPS and POPH.
    Methods We recruited patients with decompensated cirrhosis (n = 69) admitted to our hospital as liver transplantation candidates. Patients exhibiting partial pressure of oxygen lower than 80 mmHg and alveolararterial oxygen gradient (AaDO(2)) &gt;= 15 mmHg were categorized as potentially having HPS (23 of 69 patients). Patients exhibiting a tricuspid regurgitation pressure gradient &gt;= 25 mmHg were categorized as potentially having POPH (29 of 61 patients). Serum reactive oxygen metabolites were measured and anti-oxidative OXY-adsorbent test (OXY) were performed, and the balance of these tests was defined as the oxidative index. The correlation between these values and the clinical characteristics of the patients were assessed in a cross-sectional study.
    Results Potential HPS patients exhibited no correlation with oxidative stress markers. Potential POPH patients exhibited lower OXY (p = 0.037) and higher oxidative index values (p = 0.001). Additionally, the vascular NO synthase enzyme inhibiting protein, asymmetric dimethylarginine, was higher in potential POPH patients (p = 0.049). The potential POPH patients exhibited elevated AaDO(2), suggesting the presence of pulmonary shunting.
    Conclusion Potential POPH patients exhibited elevated oxidative stress with decreased anti-oxidative function accompanied by inhibited NO production. Anti-oxidants represent a candidate treatment for potential POPH patients.

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  • Severe periodontitis is inversely associated with coffee consumption in the maintenance phase of periodontal treatment. Reviewed International journal

    Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Noriko Takeuchi, Takayuki Maruyama, Shinsuke Mizutani, Kota Kataoka, Yuya Kawabata, Manabu Morita

    Nutrients   6 ( 10 )   4476 - 90   2014.10

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    This cross-sectional study addressed the relationship between coffee consumption and periodontitis in patients during the maintenance phase of periodontal treatment. A total of 414 periodontitis patients in the maintenance phase of periodontal treatment completed a questionnaire including items related to coffee intake and underwent periodontal examination. Logistic regression analysis showed that presence of moderate/severe periodontitis was correlated with presence of hypertension (Odds Ratio (OR) = 1.99, p &lt; 0.05), smoking (former, OR = 5.63, p &lt; 0.01; current, OR = 6.81, p = 0.076), number of teeth present (OR = 0.89, p &lt; 0.001), plaque control record &gt;= 20% (OR = 1.88, p &lt; 0.05), and duration of maintenance phase (OR = 1.07, p &lt; 0.01). On the other hand, presence of severe periodontitis was correlated with smoking (former, OR = 1.35, p = 0.501; current, OR = 3.98, p &lt; 0.05), coffee consumption (&gt;= 1 cup/day, OR = 0.55, p &lt; 0.05), number of teeth present (OR = 0.95, p &lt; 0.05), and bleeding on probing &gt;= 20% (OR = 3.67, p &lt; 0.001). There appears to be an inverse association between coffee consumption (&gt;= 1 cup/day) and prevalence of severe periodontitis in the maintenance phase of periodontal treatment.

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  • アルコール摂取後のブラッシング行動と歯周病の活動性との関連

    水谷 慎介, 江國 大輔, 友藤 孝明, 山根 真由, 東 哲司, 丸山 貴之, 町田 達哉, 米田 俊樹, 片岡 広太, 佐久本 雅美, 川端 勇也, 大森 智栄, 森田 学

    口腔衛生学会雑誌   64 ( 2 )   173 - 173   2014.4

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  • Occlusion and Weight Change in a Patient After Esophagectomy: Success Derived from Restoration of Occlusal Support Reviewed

    Reiko Yamanaka, Yoshihiko Soga, Mami Minakuchi, Kumiko Nawachi, Takayuki Maruyama, Takuo Kuboki, Manabu Morita

    INTERNATIONAL JOURNAL OF PROSTHODONTICS   26 ( 6 )   574 - 576   2013.11

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    Occlusal support may be an important factor affecting nutritional support after major surgery. This report presents a patient who gained body weight after receiving a new prosthesis. The patient was an 82-year-old man with thoracic esophageal carcinoma. He did not have occlusal support because of multiple caries lesions. His body weight slowly increased after surgery, but almost stopped in the period of 54 to 68 days after surgery. After treatment with dentures (day 72 postsurgery), body weight gain was observed again, although his medical treatment had not changed. An appropriate prosthesis could contribute to perioperative nutrition support and may lead to earlier recovery after surgery.

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  • Serum oxidative-anti-oxidative stress balance is dysregulated in patients with hepatitis C virus-related hepatocellular carcinoma. Reviewed International journal

    Mamoru Nishimura, Akinobu Takaki, Naofumi Tamaki, Takayuki Maruyama, Hideki Onishi, Sayo Kobayashi, Kazuhiro Nouso, Tetsuya Yasunaka, Kazuko Koike, Hiroaki Hagihara, Kenji Kuwaki, Shinichiro Nakamura, Fusao Ikeda, Yoshiaki Iwasaki, Takaaki Tomofuji, Manabu Morita, Kazuhide Yamamoto

    Hepatology research : the official journal of the Japan Society of Hepatology   43 ( 10 )   1078 - 92   2013.10

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    Aim: Oxidative stress is associated with progression of chronic liver disease (CLD). This association is best established in chronic hepatitis C. However, the anti-oxidative state is not well characterized. The objective of the present study was to investigate the balance of oxidative and anti-oxidative stress in CLD patients. Methods: We recruited a study population of 208 patients, including healthy volunteers (HV
    n=15), patients with hepatitis B virus (HBV)-related CLD without or with hepatocellular carcinoma (HBV-non-HCC, n=25, and HBV-HCC, n=50, respectively), and patients with hepatitis C virus (HCV)-related CLD without or with HCC (HCV-non-HCC, n=49, and HCV-HCC, n=69, respectively). Serum levels of reactive oxygen metabolites (ROM) and anti-oxidative markers (OXY-adsorbent test
    OXY) were determined, and the balance of these values was used as the oxidative index. Correlations among ROM, OXY, oxidative index and clinical characteristics were investigated. Results: Patients with CLD exhibited elevated ROM and oxidative index compared to HV. Among patients with CLD, HCV positive status correlated with increased ROM. In CLD, HCV-HCC patients exhibited the highest ROM levels. Among HCV-related CLD patients, lower OXY correlated with HCC positive status, but was recovered by eradication of HCC. In HCV-HCC, lower OXY correlated with high PT-INR. Conclusion: HCV positive CLD patients displayed higher oxidative stress and HCV-HCC patients displayed lower anti-oxidative state. Anti-oxidative state depression was associated with liver reservoir-related data in HCV-HCC and could be reversed with HCC eradication. © 2012 The Japan Society of Hepatology.

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  • Relationship between serum albumin concentration and periodontal condition in patients with head and neck cancer. Reviewed International journal

    Takayuki Maruyama, Reiko Yamanaka, Aya Yokoi, Daisuke Ekuni, Takaaki Tomofuji, Nobuyoshi Mizukawa, Tomoo Onoda, Motoharu Eguchi, Manabu Morita

    Journal of periodontology   83 ( 9 )   1110 - 5   2012.9

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    Background: Serum albumin concentration is known to be an independent predictor of survival in head and neck cancer. The previous studies suggested relationships between serum albumin concentration and oral health status in populations without serious systemic disorders. However, these relationships remain unclear in patients with head and neck cancer. The purpose of this study is to investigate the relationship between serum albumin concentration and oral health status in patients with head and neck cancer.
    Methods: Fifty individuals diagnosed with primary head and neck cancer, 25 individuals with normal serum albumin concentration (&gt;= 3.85 g/dL), and 25 age- and sex-matched individuals with lower serum albumin concentration (&lt;3.85 g/dL) were analyzed. General status, including cancer stage, body mass index, drinking and smoking habits, and biochemical serum markers, were evaluated. Oral health status, including periodontal condition and occlusion tooth pairs, were also evaluated.
    Results: Mean clinical attachment level (CAL) and C-reactive protein in the lower serum albumin concentration group were greater than those in the normal serum albumin concentration group (P = 0.009 and P = 0.002, respectively). However, there were no significant differences in any other oral and/or serum parameters between the two serum albumin groups. A logistic regression model showed that mean CAL was significantly associated with high or low levels of serum albumin concentration (odds ratio = 9.752; 95% confidence interval = 1.702 to 55.861; P = 0.011).
    Conclusions: This study suggests an association between periodontal disease and serum albumin concentration in patients with head and neck cancer. Longitudinal studies are necessary to examine the causal relationship between serum albumin concentration and periodontal condition. J Periodontol 2012;83:1110-1115.

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  • Relationships between periodontal inflammation, lipid peroxide and oxidative damage of multiple organs in rats. Reviewed

    Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takayuki Maruyama, Tatsuo Yamamoto, Tatsuo Watanabe, Manabu Morita

    Biomedical research (Tokyo, Japan)   32 ( 5 )   343 - 9   2011.10

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    Gingival response to periodontal inflammation generates excessive lipid peroxide and such a condition may augment systemic health through increased circulating lipid peroxide. The purpose of the present study was to investigate whether the generation of lipid peroxide in periodontal inflammation could induce tissue injury in the liver, heart, kidney and brain using a rat model. Twelve Wistar rats (8 week-old male) were divided into 2 groups: the periodontal inflammation group, receiving topical application of lipopolysaccharide and proteases to the gingival sulcus for 4 weeks, and the control group using instead pyrogen-free water. After blood samples were collected, specimens from the brain, heart, liver and kidney were resected to determine the concentration of 8-hydroxydeoxyguanosine (an indicator of oxidative DNA damage). Gingival and serum levels for hexanoyl-lysine were measured to evaluate lipid peroxide. Administration of lipopolysaccharide and proteases induced periodontal inflammation, with increasing gingival and serum levels of hexanoyl-lysine. The level of 8-hydroxydeoxyguanosine increased 2.27, 2.01, 1.49 and 1.40 times in mitochondrial DNA from the liver, heart, kidney and brain of rats with periodontal inflammation, respectively. The results reveal that excessive production of lipid peroxide following periodontal inflammation is involved in oxidative DNA damage of the brain, heart, liver and kidney.

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  • Supplementation of green tea catechins in dentifrices suppresses gingival oxidative stress and periodontal inflammation. Reviewed International journal

    Takayuki Maruyama, Takaaki Tomofuji, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Daisuke Ekuni, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    Archives of oral biology   56 ( 1 )   48 - 53   2011.1

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    Objective: This study examined the effects of a dentifrice containing green tea catechins on gingival oxidative stress and periodontal inflammation using a rat model.
    Design: Twenty-four male Wister rats were randomly divided into four groups. The first group (Control group) received no treatment for 8 weeks. Periodontal inflammation was induced in the second group for 8 weeks. Periodontal inflammation was induced in the last two groups for 8 weeks and dentifrices with or without green tea catechins were topically applied to the gingival sulcus daily for 4 weeks prior to the end of the experimental period. Results: Rats that had experimental periodontal inflammation showed apical migration of the junctional epithelium, alveolar bone loss and inflammatory cell infiltration in the connective tissue subjacent to the junctional epithelium at 8 weeks, whilst the control group showed no pathologic changes. Topical application of a green tea catechin-containing dentifrice reduced inflammatory cell infiltration in the periodontal lesions to a greater degree than the control dentifrice at 8 weeks. The gingiva in which green tea catechin-containing dentifrice was applied also showed a lower level of expression of hexanoyl-lysine (a marker of lipid peroxidation), nitrotyrosine (a marker of oxidative protein damage), and tumour necrosis factor-a (an indicator of pro-inflammatory cytokines) at 8 weeks compared to gingiva in which the control dentifrice was applied.
    Conclusions: Adding green tea catechins to a dentifrice may contribute to prevention of periodontal inflammation by decreasing gingival oxidative stress and expression of pro-inflammatory cytokines. (C) 2010 Elsevier Ltd. All rights reserved.

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  • Effects of improvement in periodontal inflammation by toothbrushing on serum lipopolysaccharide concentration and liver injury in rats. Reviewed International journal

    Takaaki Tomofuji, Daisuke Ekuni, Toshihiro Sanbe, Tetsuji Azuma, Naofumi Tamaki, Koichiro Irie, Takayuki Maruyama, Tatsuo Yamamoto, Tatsuo Watanabe, Mutsumi Miyauchi, Takashi Takata

    Acta odontologica Scandinavica   67 ( 4 )   200 - 5   2009.7

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    Objective. Periodontitis increases the serum lipopolysaccharide level, contributing to liver injury. Toothbrushing improves periodontitis and may also affect serum lipopolysaccharide concentration and periodontitis-induced liver injury. The purpose of the present study was to examine whether the improvement in periodontal inflammation by toothbrushing clinically affects the serum lipopolysaccharide level and hepatic pathological changes in rat periodontitis. Material and methods. Thirty male Wistar rats were divided into 5 groups, 2 groups receiving topical application of pyrogen-free water to the gingival sulcus for 4 or 8 weeks. The next 2 groups received topical application of lipopolysaccharide and proteases for 4 or 8 weeks. The last group received topical application of lipopolysaccharide and proteases for 8 weeks, and the palatal gingiva was brushed with a powered toothbrush once a day for 4 weeks prior to the end of the experimental period. Results. Topical application of lipopolysaccharide and proteases induced not only periodontal inflammation but also an elevation in the serum lipopolysaccharide concentration, with increasing hepatic inflammation, steatosis and 8-hydroxydeoxyguanosine levels in a time-dependent mariner. The rats that received gingival stimulation showed decreased polymorphonuclear leukocyte infiltration and collagen loss levels in the periodontal lesions. Furthermore, this group also showed a decrease in serum lipopolysaccharide concentration and hepatic inflammation, steatosis and 8-hydroxydeoxyguanosine levels, compared with the group receiving no treatment. Conclusions. Toothbrushing promoted healing of periodontal lesions, decreased serum lipopolysaccharide concentration and suppressed liver injury in a rat periodontitis model.

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  • Vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress. Reviewed International journal

    Daisuke Ekuni, Takaaki Tomofuji, Toshihiro Sanbe, Koichiro Irie, Tetsuji Azuma, Takayuki Maruyama, Naofumi Tamaki, Jun Murakami, Susumu Kokeguchi, Tatsuo Yamamoto

    Archives of oral biology   54 ( 5 )   495 - 502   2009.5

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    Objective: Periodontitis has been causally linked to cardiovascular disease, which is mediated through the oxidative stress induced by periodontitis. Since vitamin C has been suggested to limit oxidative damage, we hypothesized that vitamin C intake may reduce endothelial oxidative stress induced by periodontitis in the aorta. The aim of this study was to investigate the effects of vitamin C intake on the initiation of atherosclerosis in a ligature-induced rat periodontitis model.
    Design: Eighteen 8-week-old-male Wistar rats were divided into three groups of six rats and all rats received daily fresh water and powdered food through out the 6-week study. In the vitamin C and periodontitis groups, periodontitis was ligature-induced for the first 4 weeks. In the vitamin C group, rats were given distilled water containing 1 g/L vitamin C for the 2 weeks after removing the ligature.
    Results: In the periodontitis group, there was lipid deposition in the descending aorta and significant increases of serum level of hexanoyl-lysine (HEL), and aortic levels of nitrotyrosine expression, HEL expression and 8-hydroxydeoxyguanosine (8-OHdG) compared to the control group. Vitamin C intake significantly increased plasma vitamin C level and GSH:GSSG ratio (178% and 123%, respectively), and decreased level of serum HEL and aortic levels of nitrotyrosine, HEL and 8-OHdG (23%, 87%, 84%, and 38%, respectively).
    Conclusions: These results suggest that vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress. (C) 2009 Elsevier Ltd. All rights reserved.

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  • Validity of a questionnaire for periodontitis screening of Japanese employees. Reviewed International journal

    Tatsuo Yamamoto, Reiko Koyama, Naofumi Tamaki, Takayuki Maruyama, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Tetsuji Azuma, Manabu Morita

    Journal of occupational health   51 ( 2 )   137 - 43   2009.3

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    Validity of a Questionnaire for Periodontitis Screening of Japanese Employees: Tatsuo YAMAMOTO, et al. Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences-Objectives: Workplaces are suitable for screening for periodontal disease effectively. However, the majority of workplaces do not have a screening program. One possible reason may be that pocket probing, which is commonly used for the screening, needs qualified examiners and is time-consuming. In this study, the validity of a self-reported questionnaire was assessed for screening of periodontitis in 50- to 59-yr-old male employees in Japan. Methods: A total of 250 people were asked to complete a self-administered questionnaire regarding signs and symptoms of periodontal disease, and experience of periodontal treatment, followed by a clinical examination by a dentist. Subjects with at least one tooth having a clinical attachment loss of 7 mm or more were defined as periodontitis patients. Results: Thirteen percent of the subjects were diagnosed as having periodontitis. Logistic regression selected 4 questions, "Are you a current or past smoker?", "Have your gums bled recently?", "Do you think that you can see more roots of teeth than in the past?", and "Have you ever been told that you need periodontal or gum treatment?" as potential predictors of periodontitis. When subjects with at least 3 "yes" responses to the 4 questions were separated from the others, the subjects with periodontitis were separated most effectively (showing the highest sensitivity + specificity: 1.524) from those without. The area under the receiver operating characteristic curve was 0.81. Conclusions: These results suggest that the self-reported questions are useful for screening of periodontitis in 50- to 59-yr-old Japanese workers.

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  • Effects of vitamin C intake on gingival oxidative stress in rat periodontitis. Reviewed International journal

    Takaaki Tomofuji, Daisuke Ekuni, Toshihiro Sanbe, Koichiro Irie, Tetsuji Azuma, Takayuki Maruyama, Naofumi Tamaki, Jun Murakami, Susumu Kokeguchi, Tatsuo Yamamoto

    Free radical biology & medicine   46 ( 2 )   163 - 8   2009.1

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    Increased levels of oxidative stress due to excessive production of reactive oxygen species are involved in the pathogenesis of periodontitis. Studies Suggest a negative association between plasma vitamin C level and the severity of periodontitis. We hypothesized that increases in plasma vitamin C levels after vitamin C intake might clinically reduce gingival oxidative stress in a rat periodontitis model. A ligature was placed around rat mandibular molars for 4 weeks to induce periodontitis, and the rats were then given drinking water with or without 1 g/L vitamin C for 2 weeks after the ligature was removed. The periodontitis-induced rats showed a 149% increase in 8-hydroxydeoxyguanosine level and a 40% decrease in reduced:oxidized glutathione ratio in gingival tissue. Vitamin C intake induced a 175% increase in plasma vitamin C level, resulting in an improvement in the gingival 8-hydroxydeoxyguanosine level (decreased) and in the reduced:oxidized glutathione ratio (increased). Furthermore, in ligature-induced periodontitis lesions, gene expression encoding inflammation, including interleukin-1 alpha and interleukin-1 beta, was more than twofold down-regulated by vitamin C intake. The results suggest that systemic administration of vitamin C could be clinically beneficial in improving periodontitis-induced oxidative stress by down-regulating inflammatory gene expression. (C) 2008 Elsevier Inc. All rights reserved.

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  • Oxidative damage of rat liver induced by ligature-induced periodontitis and chronic ethanol consumption. Reviewed International journal

    Takaaki Tomofuji, Toshihiro Sanbe, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Takayuki Maruyama, Naofumi Tamaki, Tatsuo Yamamoto

    Archives of oral biology   53 ( 12 )   1113 - 8   2008.12

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    Objective: oxidative stress plays a central role in the initiation and progression of liver disease. Chronic ethanol consumption induces oxidative damage of the liver. Using a rat model, we previously showed that chronic administration of lipopolysaccharide and proteases to gingival sulcus induced both periodontal inflammation and liver injury. Periodontitis and ethanol consumption may have an additional effect on hepatic oxidative damage. The present study investigated the effects of periodontitis on ethanol-induced oxidative damage of the liver using a rat model.
    Design: Male Wistar rats were divided into four groups (six rats/group). During the experimental period of eight weeks, two groups were fed an ethanol-containing liquid diet, and two groups were on a pair-fed control diet. Four weeks prior to the end of the experimental period, one group from each dietary treatment was ligated to induce periodontitis, while the other group was left unligated. In order to evaluate hepatic oxidative damage, the level of hexanoyl-lysine and the ratio of reduced form glutathione/oxidized form glutathione (GSH/GSSG) was determined. The concentration of blood hexanoyl-lysine was also measured as an index of circulating lipid peroxide.
    Results: Ligature-induced periodontitis increased plasma levels of hexanoyl-lysine. in the liver, periodontitis decreased the CSH/GSSG ratio, and the combination of periodontitis and ethanol consumption induced a significant increase in hexanoyl-lysine level compared to ethanol consumption alone.
    Conclusion: In the rat model, ligature-induced periodontitis increased plasma lipid peroxide, decreased the hepatic GSH/GSSG ratio and augmented ethanol-induced lipid peroxidation in the liver. (C) 2008 Elsevier Ltd. All rights reserved.

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  • Relationship between periodontal condition and plasma reactive oxygen metabolites in patients in the maintenance phase of periodontal treatment. Reviewed International journal

    Naofumi Tamaki, Takaaki Tomofuji, Takayuki Maruyama, Daisuke Ekuni, Reiko Yamanaka, Noriko Takeuchi, Tatsuo Yamamoto

    Journal of periodontology   79 ( 11 )   2136 - 42   2008.11

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    Background: The relationship between systemic antioxidative status and periodontal condition has been investigated in epidemiologic studies. However, little literature is available with regard to the correlation between systemic reactive oxygen species and periodontal condition. The purpose of this cross-sectional study was to investigate the relationship between plasma reactive oxygen metabolites (ROM) and periodontal condition in patients in the maintenance phase of periodontal treatment.
    Methods: Eighty-one subjects (mean age: 57.4 years) who had entered a periodontal maintenance program were examined for probing depth (PD) and clinical attachment level (CAL). Plasma levels of ROM and biologic antioxidant potential (BAP) were determined with a free radical electric evaluator.
    Results: The plasma level of ROM was positively correlated to mean CAL (r = 0.281; P = 0.011) and percentage of teeth with CAL A mm (r = 0.236; P = 0.034), but not mean PD (r = 0.196; P = 0.080). Logistic regression analysis showed that subjects with ROM levels &gt;400 Carratelli units (CARR U) had significantly higher mean CAL compared to subjects with ROM &gt;400 CARR U after adjusting for age, gender, and the number of teeth present (P = 0.011). However, the plasma level of BAP was not significantly correlated with the periodontal parameters.
    Conclusions: A positive association was found between plasma oxidative status and CAL in patients in the maintenance phase of periodontal therapy. A systemic increase in oxidative stress may influence the rate of progression of periodontal disease. J Periodontol 2008; 79:2136-2142.

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  • Effects of Ethanol Consumption on Periodontal Inflammation in Rats Reviewed

    K. Irie, T. Tomofuji, N. Tamaki, T. Sanbe, D. Ekuni, T. Azuma, T. Maruyama, T. Yamamoto

    Journal of Dental Research   87 ( 5 )   456 - 460   2008.5

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  • Relationship between periodontal status and levels of oxidative stress and antioxidant potential in plasma

    Maruyama Takayuki, Tamaki Naofumi, Tomofuji Takaaki, Ekuni Daisuke, Yamanaka Reiko, Takeuchi Noriko, Yamamoto Tatsuo

    Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology   2008   52 - 52   2008

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  • 日中の咬筋の筋活動は歯周炎の進行と関係する:コホート研究

    江國大輔, 加藤聖也, 川上滋央, 丸山貴之, 片岡広太, 内田瑤子, 福原大樹, 外山直樹, 皆木省吾, 森田学

    日本疫学会学術総会講演集(Web)   30th   2020

  • 歯周病ラットにおける血清miRNAの肝臓mRNAへの影響

    杉浦 嘉雄, 米田 俊樹, 藤森 浩平, 丸山 貴之, 宮井 久敬, 小林 暉政, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   69 ( 増刊 )   182 - 182   2019.4

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  • ラット歯周病モデルにおける血清miRNAと肝臓への影響

    杉浦 嘉雄, 米田 俊樹, 藤森 浩平, 丸山 貴之, 宮井 久敬, 小林 暉政, 江國 大輔, 友藤 孝明, 森田 学

    岡山歯学会雑誌   37 ( 2 )   81 - 81   2018.12

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  • 化学放射線療法中の口腔粘膜炎管理を行った症例

    益成 美保, 水野 裕文, 丸山 貴之, 横井 彩, 小林 暉政, 佐々木 禎子, 志茂 加代子, 三浦 留美, 水川 展吉, 江國 大輔, 森田 学

    口腔衛生学会雑誌   68 ( 4 )   231 - 237   2018.10

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    頭頸部がんに対する化学放射線療法(CRT)の有害事象である口腔粘膜炎による疼痛は、患者のQOLを低下させ、がん治療の完遂を困難にする。今回、われわれは術後CRTを受けている患者に対して口腔管理を行い、口腔粘膜炎および疼痛を制御した結果、がん治療の完遂に貢献できた症例を報告する。患者は67歳、男性である。右側舌がん(T2N2bM0)に対して舌可動部半側切除術、右側頸部郭清術、遊離前外側大腿皮弁による再建術を施行した。術後の病理組織検査より切除断端陽性、頸部リンパ節の節外浸潤を認めたため、術後CRT(抗がん剤:シスプラチン、5-フルオロウラシル;2クール、強度変調放射線治療60Gy)を行うこととなった。CRT前から、口腔管理および衛生実地指導を継続して行った。CRT開始時はプラークの除去を中心とした口腔管理を行った。22Gy照射時点で口腔粘膜炎(Grade2)が発症したものの、その後軽度疼痛を認める程度で経過していた。42Gy照射時点で口腔粘膜炎の悪化(Grade3)および自発痛・接触痛を認めたため、治療完遂まで毎日、歯科衛生士がプラークの除去に加え、口腔粘膜保護を目的とした口腔管理を行った。その結果、56Gy照射時点で口腔粘膜炎は改善傾向を示し(Grade2)、疼痛も緩和され、CRT完遂に至った。術後CRT開始直後から口腔粘膜炎の状態に応じた口腔管理を行うことで、疼痛の制御が成功し、CRTの完遂に貢献できたと考えられる。(著者抄録)

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  • 歯周組織における加齢に伴う酸化ストレスの増加に対するコーヒー摂取の影響

    小林 暉政, 東 哲司, 杉浦 嘉雄, 宮井 久敬, 米田 俊樹, 丸山 貴之, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   68 ( 増刊 )   182 - 182   2018.4

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  • 抗がん剤投与ラットへのカテキン局所塗布による抗酸化作用の検討

    小林 暉政, 宮井 久敬, 丸山 貴之, 友藤 孝明, 東 哲司, 米田 俊樹, 杉浦 嘉雄, 江國 大輔, 森田 学

    口腔衛生学会雑誌   67 ( 3 )   203 - 203   2017.7

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  • 唾液中microRNAと歯周病の重症度との関連

    藤森 浩平, 米田 俊樹, 友藤 孝明, 江國 大輔, 東 哲司, 丸山 貴之, 町田 達哉, 水野 裕文, 森田 学

    口腔衛生学会雑誌   67 ( 増刊 )   168 - 168   2017.4

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  • 2型糖尿病患者における非外科的歯周治療による酸化ストレスの改善 ランダム化比較試験

    杉浦 嘉雄, 水野 裕文, 福原 大樹, 丸山 貴之, 江國 大輔, 米田 俊樹, 片岡 広太, 川端 勇也, 水谷 慎介, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   67 ( 増刊 )   119 - 119   2017.4

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  • 本邦のさまざまな医療機関における倫理委員会の現状と課題 臨床研究が効率よく実施されるために

    藤原 智洋, 藤井 政孝, 吉原 久美子, 大野 彩, 三橋 利晴, 丸山 貴之, 桑木 健司, 伊東 孝, 難波 志穂子, 尾崎 敏文, 樋之津 史郎

    日本整形外科学会雑誌   91 ( 2 )   S303 - S303   2017.3

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  • 口腔内アセトアルデヒド濃度と舌背の細菌数との関係

    加藤 壮真, 吉田 宗生, 横井 彩, 丸山 貴之, 江國 大輔, 山根 真由, 山中 玲子, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   66 ( 2 )   296 - 296   2016.4

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  • 食道癌の術後患者に対する歯科専門職による口腔のケアの効果

    水野 裕文, 水谷 慎介, 丸山 貴之, 横井 彩, 谷口 彩乃, 山中 玲子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   66 ( 2 )   219 - 219   2016.4

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  • 歯肉炎のスクリーニングにおける唾液中LDH簡易測定キットの有効性

    吉田 宗生, 加藤 壮真, 山根 真由, 江國 大輔, 横井 彩, 丸山 貴之, 山中 玲子, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   66 ( 2 )   294 - 294   2016.4

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  • 口腔ケアの感染症予防・治療への有効性 上部消化癌患者と健常者における舌苔と口腔内アセトアルデヒド濃度との関連

    山中 玲子, 横井 彩, 丸山 貴之, 江國 大輔, 森田 学

    日本化学療法学会雑誌   64 ( 2 )   314 - 315   2016.3

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  • 新しい統合指針の導入により倫理審査体制はどう変化したか 倫理審査を兼任する整形外科医が読み取る倫理申請の現状とこれから

    藤原 智洋, 吉原 久美子, 大野 彩, 三橋 利晴, 丸山 貴之, 桑木 健志, 加藤 有加, 伊東 孝, 難波 志穂子, 樋之津 史郎, 尾崎 敏文

    日本整形外科学会雑誌   90 ( 3 )   S657 - S657   2016.3

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  • 口腔ケアの感染症予防・治療への有効性 上部消化癌患者と健常者における舌苔と口腔内アセトアルデヒド濃度との関連

    山中 玲子, 横井 彩, 丸山 貴之, 江國 大輔, 森田 学

    感染症学雑誌   90 ( 2 )   168 - 169   2016.3

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  • 歯周病メインテナンス期患者に対する血液検査による生活習慣病スクリーニングの検討

    町田 達哉, 友藤 孝明, 江國 大輔, 東 哲司, 竹内 倫子, 丸山 貴之, 川端 勇也, 森田 学

    口腔衛生学会雑誌   65 ( 4 )   354 - 361   2015.7

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    生活習慣病予防はわが国喫緊の課題である。生活習慣病スクリーニングを受ける機会の提供、生活習慣病に関する情報提供、および、医療機関への適切な受診勧奨等が生活習慣病予防のために重要である。本研究では、これらを実施する場の一つとして歯科を捉え、その可能性を検討することを目的とした。簡易な血液検査による生活習慣病スクリーニングを歯周病メインテナンス期患者に行い、分析した。2014年1月から7月に岡山大学病院予防歯科外来を受診した歯周病メインテナンス期患者63名(男性22名、女性41名、平均年齢65.9±6.7歳)を対象に、自己記入式質問紙調査と指尖血の採取を行い、肝疾患、高脂血症(脂質異常症)、腎疾患、痛風、糖尿病前症、および糖尿病の疑いの有無を判定した。過去1年間の医療機関受診歴を53名(84.1%)が有していた。過去1年間に肝疾患、高脂血症、腎疾患、痛風、および糖尿病の診断のある者は、それぞれ3名、14名、2名、1名、3名おり、各々の分析から除外された。血液検査の結果、肝疾患、高脂血症、腎疾患、痛風、糖尿病前症、および糖尿病の疑いは、それぞれ対象者の16.7%、34.7%、16.4%、1.6%、46.7%、および6.7%に認められた。医療機関受診歴を有する者の多さに関わらず、生活習慣病予防が必要と思われる者の割合は十分多かった。歯科の場において、簡易な生活習慣病スクリーニングを行い、医療機関への受診勧奨等を行う流れを作ることで、国民の生活習慣病予防の一端を担えるかもしれない。(著者抄録)

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  • 多職種連携の中で専門的口腔衛生管理を行い、経口摂取を継続しながら化学放射線治療が完遂できた一症例

    志茂 加代子, 丸山 貴之, 横井 彩, 山中 玲子, 三浦 留美, 江國 大輔, 森田 学

    口腔衛生学会雑誌   65 ( 2 )   187 - 187   2015.4

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  • 岡山大学病院周術期管理センターにおける術前プラークフリーの実際

    丸山 貴之, 水谷 慎介, 山中 玲子, 横井 彩, 町田 達哉, 米田 俊樹, 片岡 広太, 川端 勇也, 森田 学

    口腔衛生学会雑誌   65 ( 3 )   312 - 312   2015.4

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  • Self-efficacy and progression of periodontal disease: a prospective cohort study. International journal

    Shinsuke Mizutani, Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

    Journal of clinical periodontology   42 ( 12 )   1083 - 1089   2015

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    AIM: The purpose of this longitudinal study was to investigate whether self-efficacy scale for self-care (SESS) was related to changes in oral health behaviours and progression of periodontal disease in Japanese university students. MATERIAL AND METHODS: Students (n = 533) who were interested in receiving oral health examinations before entering the university and before graduation were included in the analysis. Self-efficacy was assessed using SESS, which uses three subscales: self-efficacy for brushing of the teeth (SE-BR), for dentist consultations (SE-DC) and for dietary habits. The informations about oral health behaviours were also collected. For all participants, oral hygiene instructions was performed at baseline. After 3 years, the oral health behaviours and the periodontal condition were re-assessed. RESULTS: The progression of periodontal disease was related to low baseline SE-BR. Logistic regression analysis showed that progression of periodontal disease risk during a 3-year period was associated with low baseline SE-BR (OR: 1.516; 95% CI: 1.010-2.275; p < 0.05). The increase in the score of SE-DC was related to the increase in regular dental checkups (p < 0.05). CONCLUSION: Low SESS was associated with progression of periodontal disease and improvement of oral health behaviours in university students who received oral hygiene instructions at baseline.

    DOI: 10.1111/jcpe.12476

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  • 若年者における急性上気道炎の発症頻度と智歯萠出状態との関連

    町田 達哉, 水谷 慎介, 片岡 広太, 山根 真由, 丸山 貴之, 東 哲司, 友藤 孝明, 江國 大輔, 森田 学

    口腔衛生学会雑誌   64 ( 4 )   328 - 335   2014.7

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    口腔状態と気道感染の関連性の議論が近年盛んである。口腔は上気道と接しており、半萠出智歯の存在は、隣在歯との間に清掃困難な空間を形成し、智歯周囲の細菌量を増加させ、上気道を含む周囲組織に影響を与えうる。そこで、対象者を智歯萠出時期である18-20歳の大学生とし、急性上気道炎の発症頻度と智歯萠出状態の関連を検討することとした。2013年岡山大学新入生健康診断の際に歯科健診を任意受診した18-67歳(平均年齢18.5±2.1歳)の2,205名中、非喫煙者でデータ欠損のない18-20歳の1,964名(男性1,130名、女性834名)を分析対象とした。口腔診査を行い口腔清掃度、地域歯周疾患指数、および智歯萠出状態を調べた。自己記入式質問調査により過去1年間の急性上気道炎の発症頻度を尋ね、年間3回以上発症した群と3回未満発症した群の2群に分けた。χ2検定で2群比較を行い、有意な関連を智歯萠出状態と過去1年間の一番奥の歯ぐきの痛みの既往で認めた(P&lt;0.05)。口腔清掃度にも一定の関連性を認めた(P=0.054)。性別、口腔清掃度、智歯萠出状態、過去1年間の一番奥の歯ぐきの痛みの既往を説明変数としたロジスティック回帰分析を行ったところ、萠出智歯なしの者に対し、半萠出智歯を有する者は急性上気道炎を過去1年間に3回以上発症していた者の割合が有意に高かった(P=0.003)。大学生において智歯萠出状態と急性上気道炎の発症頻度に関連性があることが示唆された。(著者抄録)

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  • 頭頸部がん患者を支えるチーム医療 岡山大学病院頭頸部がんセンターにおける口腔ケア

    丸山 貴之, 志茂 加代子, 佐々木 禎子, 田中 千加, 横井 彩, 水谷 慎介, 山中 玲子, 森田 学

    口腔衛生学会雑誌   64 ( 3 )   316 - 316   2014.4

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  • 若年者における血圧と歯周炎との関連についてのコホート研究

    川端 勇也, 江國 大輔, 友藤 孝明, 東 哲司, 丸山 貴之, 水谷 慎介, 米田 俊樹, 町田 達哉, 片岡 広太, 山根 真由, 佐久本 雅美, 大森 智栄, 森田 学

    口腔衛生学会雑誌   64 ( 2 )   175 - 175   2014.4

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  • アルコール摂取後の口腔保健行動の変化と歯周病との関連

    山根 真由, 江國 大輔, 水谷 慎介, 町田 達哉, 片岡 広大, 丸山 貴之, 森田 学

    岡山歯学会雑誌   32 ( 2 )   87 - 88   2013.12

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  • 頭頸部がん患者に対する歯科衛生士の取り組みと今後の展望

    佐々木 禎子, 志茂 加代子, 田中 千加, 三浦 留美, 山中 玲子, 丸山 貴之, 横井 彩, 水川 展吉, 森田 学, 宮脇 卓也

    岡山歯学会雑誌   32 ( 2 )   81 - 81   2013.12

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  • 若年者における風邪の発症頻度と智歯の萠出状態との関連

    町田 達哉, 水谷 慎介, 片岡 広太, 山根 真由, 丸山 貴之, 友藤 孝明, 江國 大輔, 森田 学

    岡山歯学会雑誌   32 ( 2 )   87 - 87   2013.12

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  • 頭頸部がん患者に対して口腔ケアを行った2症例

    丸山 貴之, 山中 玲子, 志茂 加代子, 田中 千加, 曽我 賢彦, 森田 学

    日本歯周病学会会誌   55 ( 3 )   262 - 268   2013.9

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    頭頸部癌は他部位の癌と異なり、細菌の多い口腔が領域に含まれるのが特徴である。したがって、細菌感染による合併症の発症リスクが高い。頭頸部癌治療に対する口腔ケアは合併症の予防・軽減に有効である。岡山大学病院予防歯科では、多職種がチーム医療を展開する頭頸部がんセンターが設立されている。同院予防歯科では周術期および退院後の継続的な口腔ケアを担当している。その中から2症例を紹介した。

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  • 頭頸部がん患者における予後推定栄養指数と口腔内状態との関係

    丸山 貴之, 友藤 孝明, 江國 大輔, 水谷 慎介, 森田 学

    口腔衛生学会雑誌   63 ( 4 )   321 - 327   2013.7

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    頭頸部がん治療の予後を知るうえで、小野寺の予後推定栄養指数(O-PNI)は重要な指標であるが、O-PNIと栄養摂取器官である口腔内の状態との関連について検討したものはない。本研究の目的は、頭頸部がん患者におけるO-PNIと口腔内状態との関連を調べることである。嚥下機能への影響を考慮するため、がんの発生部位を口腔内と口腔外に分け、それぞれのO-PNIと口腔内状態との関連について比較検討した。岡山大学病院にて頭頸部がんと診断を受けたがん治療開始前の150名を分析対象とした。がんの部位、進行度(ステージ)、body mass index、飲酒経験、喫煙量、肝機能、腎機能、C反応性タンパク(CRP)、口腔内指標を調べた。口腔内外それぞれのがんにおいて、O-PNIと各指標との重回帰分析を行い、O-PNIとの関連因子について検討した。重回帰分析の結果、口腔がん患者においてはO-PNIと年齢(p=0.017)、平均クリニカルアタッチメントレベル(p=0.049)、CRP(p&lt;0.001)との間に関連を認めた。一方、口腔がん以外の頭頸部がん患者においてはO-PNIとCRP(p=0.005)との間に関連を認めたが、口腔内指標との関連は認められなかった。以上より、頭頸部がん患者におけるO-PNIを左右する因子として、口腔内のがん患者については歯周状態が関連しているのに対し、口腔外のがん患者については口腔内指標との関連はなかった。(著者抄録)

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  • 頭頸部がんセンターにおける定期的な病棟往診の役割

    丸山 貴之, 志茂 加代子, 佐々木 禎子, 水谷 慎介, 児島 梓, 米田 俊樹, 町田 達哉, 横井 彩, 山中 玲子, 森田 学

    岡山歯学会雑誌   31 ( 2 )   91 - 92   2012.12

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  • 小学生における永久歯の萠出状況の報告

    横井 彩, 山中 玲子, 江國 大輔, 丸山 貴之, 入江 浩一郎, 遠藤 康正, 水谷 慎介, 友藤 孝明, 森田 学

    岡山歯学会雑誌   31 ( 1 )   39 - 40   2012.6

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  • 耳鼻咽喉科病棟看護師を対象に口腔ケアに関するアンケートの実施とその報告

    志茂 加代子, 岡崎 惠子, 三浦 留美, 山中 玲子, 水川 展吉, 江国 大輔, 丸山 貴之, 横井 彩, 仲田 直樹, 武田 斉子, 喜田 沙音里, 森田 学

    岡山歯学会雑誌   31 ( 1 )   34 - 35   2012.6

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  • 小学生における永久歯の萠出状況の報告

    横井 彩, 山中 玲子, 江國 大輔, 丸山 貴之, 入江 浩一郎, 遠藤 康正, 水谷 慎介, 友藤 孝明, 森田 学

    岡山歯学会雑誌   30 ( 2 )   87 - 87   2011.12

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  • 耳鼻咽喉科病棟看護師を対象に口腔ケアに関するアンケートの実施とその報告

    志茂 加代子, 岡崎 惠子, 三浦 留美, 山中 玲子, 水川 展吉, 江国 大輔, 丸山 貴之, 横井 彩, 仲田 直樹, 武田 斉子, 喜田 沙音里, 森田 学

    岡山歯学会雑誌   30 ( 2 )   82 - 82   2011.12

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  • 小学生における視力と口唇閉鎖力の関係(Relationship between Visual Acuity and Labial Closure Force in Japanese Elementary School Children)

    山中 玲子, アクター・ラヘナ, 古田 美智子, 江國 大輔, 江崎 光恵, 山本 龍生, 岡崎 好秀, 丸山 貴之, 横井 彩, 森田 学

    口腔衛生学会雑誌   61 ( 3 )   288 - 294   2011.7

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    口唇閉鎖力は、主に口輪筋の機能を反映している。口輪筋は発音や咀嚼などさまざまな口腔機能に関連している。口唇閉鎖力は咀嚼能力や不正咬合に関連がある。一方、裸眼視力1.0未満(&lt;20/20)の小学生は、日本においてこの20年間増え続けている。小学生の視力低下を予防することは、重要な課題である。視力は眼輪筋の強さに関連する可能性がある。眼輪筋は口輪筋とともに顔面筋の一つであり、両者ともに顔面神経の支配を受けており、頬筋などを介して機械的にもつながっており、協調して運動する。口輪筋の強さは、眼輪筋の強さを反映していると推測される。本研究では、口唇閉鎖力が視力に関連しているという仮説を立て、小学生の視力と口唇閉鎖力の関連性を横断的に検討することを目的とした。対象は岡山市内の7歳から12歳までの小学生396名(男子197名、女子199名)とした。口腔内状態と視力は、定期健康診断の結果を用いた。口唇閉鎖力は、LIP DE CUM/LDC-110(コスモ計器、東京)を用いて測定した。典型的な不正咬合を有する16名を除外し、高視力群(≧20/20)264名と低視力群(&lt;20/20)116名に分けた。統計分析には、Mann-Whitney U検定、χ2検定、ロジスティック回帰分析を用いた。高視力群では、口唇閉鎖力(p=0.024)と男子の割合(p=0.045)が有意に高く、年齢(p=0.001)とヘルマンの歯齢(p=0.002)が有意に低かった。性別、年齢、ヘルマンの歯齢で調整しても、低視力群では口唇閉鎖力が有意に低かった(OR 1.65、95% CI 1.04-2.64、p=0.004)。小学生において、視力と口唇閉鎖力には関連があることが示唆された。(著者抄録)

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  • 頭頸部癌患者における口腔内状態と血清アルブミン値の関連

    山中 玲子, 丸山 貴之, 横井 彩, 森田 学, 水川 展吉, 小野田 友男, 江口 元治

    頭頸部癌   37 ( 2 )   220 - 220   2011.5

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  • 化学放射線療法を実施した頭頸部癌患者における、口腔粘膜炎と、C反応性タンパク、酸化ストレスの関係

    横井 彩, 山中 玲子, 丸山 貴之, 玉木 直文, 森田 学

    日本口腔ケア学会雑誌   5 ( 1 )   109 - 109   2011.1

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  • 小学生における視力と口唇閉鎖力の関係

    山中 玲子, 古田 美智子, 江崎 光恵, 江國 大輔, 丸山 貴之, 岡崎 好秀, 森田 学

    口腔衛生学会雑誌   60 ( 5 )   606 - 606   2010.10

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  • 頭頸部がん患者における口腔内の状態と血清アルブミン値の関係

    丸山 貴之, 山中 玲子, 横井 彩, 古田 美智子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   60 ( 4 )   461 - 461   2010.8

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  • 周術期における歯科介入の重要性 岡山大学病院周術期管理センター歯科部門の取り組み

    丸山 貴之, 山中 玲子, 曽我 賢彦, 縄稚 久美子, 柳 文修, 兒玉 直紀, 中田 貴, 村田 尚道, 有岡 享子, 山根 美榮子, 三浦 留美, 羽川 操, 木南 美香, 高橋 明子, 住吉 由季子, 竹内 哲男, 窪木 拓男, 佐々木 朗, 森田 学

    日本口腔ケア学会雑誌   4 ( 1 )   83 - 83   2010.3

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    J-GLOBAL

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  • ワークショップ「頭頸部がんチーム医療における口腔ケアの促進」と連携強化.

    山中玲子, 丸山貴之, 横井 彩, 山田小百合, 玉木直文, 江國大輔, 友藤孝明, 森田 学

    岡山歯学会雑誌   61   2010

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  • 急性期病院における頭頸部癌患者への口腔ケア

    山中 玲子, 小山 玲子, 江國 大輔, 古田 美智子, 丸山 貴之, 山本 龍生, 森田 学

    口腔衛生学会雑誌   59 ( 5 )   609 - 610   2009.10

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  • アンケートによる歯周疾患スクリーニングの有効性

    小山 玲子, 玉木 直文, 丸山 貴之, 友藤 孝明, 江國 大輔, 山中 玲子, 東 哲司, 山本 龍生, 森田 学

    岡山歯学会雑誌   28 ( 1 )   88 - 88   2009.6

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  • Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis

    D. Ekuni, T. Tomofuji, T. Sanbe, K. Irie, T. Azuma, T. Maruyama, N. Tamaki, J. Murakami, S. Kokeguchi, T. Yamamoto

    Journal of Periodontal Research   44 ( 4 )   434 - 442   2009

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  • アンケートによる歯周疾患スクリーニングの有効性

    小山 玲子, 玉木 直文, 丸山 貴之, 友藤 孝明, 江國 大輔, 山中 玲子, 東 哲司, 山本 龍生

    口腔衛生学会雑誌   58 ( 5 )   557 - 557   2008.10

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  • 歯周炎モデルラットにおける歯肉酸化ストレス度・抗酸化力に対するビタミンC全身投与の効果

    入江 浩一郎, 友藤 孝明, 江國 大輔, 三部 俊博, 東 哲司, 丸山 貴之, 玉木 直文, 山本 龍生, 森田 学

    口腔衛生学会雑誌   58 ( 4 )   454 - 454   2008.8

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  • 歯周組織の状態と血漿の酸化ストレス度・抗酸化力との関係

    丸山 貴之, 玉木 直文, 友藤 孝明, 江國 大輔, 山中 玲子, 竹内 倫子, 山本 龍生

    日本歯周病学会会誌   50 ( 春季特別 )   137 - 137   2008.4

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Research Projects

  • 摂食嚥下療法は頭頸部がんサバイバーの復職支援となりうるか?

    Grant number:21K10209  2021.04 - 2024.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    横井 彩, 丸山 貴之, 江國 大輔, 森田 学, 山中 玲子

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    Grant amount:\3640000 ( Direct expense: \2800000 、 Indirect expense:\840000 )

    本研究の目的は、頭頸部がんに対する治療を終了した頭頸部がんサバイバーにおいて、①術後の早期復職に関連する口腔機能の特定と、②術後早期からの嚥下訓練による早期復職・QOLへの影響について明らかにすることである。
    令和3年度では、頭頸部がんサバイバーにおいて就労状況と、QOL、口腔機能との関係について明らかにした。具体的には、口腔ケア目的に予防歯科外来を受診した頭頸部がんサバイバーに対し、以下の項目について評価した。1,就労状況(就労日数・時間)、2,QOL(自己記述式アンケート)、3,口腔機能(舌を押し上げる力、口の開き具合、口の中の粘膜の水分量、かみ合わせ、舌上の細菌の量、自己記述式アンケート)。対象者95名のうち、39名ががん治療後に復職していた。仕事をしているがんサバイバー28名と、仕事をしていないがんサバイバー28名との間で、QOLと口腔機能を比較した。
    統計解析の結果、仕事をしていないがんサバイバーは、仕事をしているがんサバイバーと比較し、再発や転移などで再手術を受けた者の割合が高く、QOLが低かった。また、仕事をしていないがんサバイバーは、仕事をしているがんサバイバーと比較し、嚥下機能の指標となる「舌を押し上げる力(舌圧)」は同程度であるものの、口腔機能に関するアンケート調査において、「嚥下機能に問題がある」と答えた者の割合が高かった。

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  • Regulation of microRNAs that transmit information from gingival tissue to systemic organs

    Grant number:21K10207  2021.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    森田 学, 丸山 貴之, 江國 大輔, 友藤 孝明, 片岡 広太

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    歯周病は、歯周組織の炎症性疾患である。また、歯周病は様々な全身疾患に影響することが知られている。しかし、歯周病が、どのような機序で全身臓器に影響するかについては不明な点が多い。
    microRNAは、17~25塩基対からなる短鎖ノンコーディングRNAであり、互いに部分相補的な遺伝子配列を持つメッセンジャー RNA(mRNA)に結合し、その遺伝子発現を制御する。本研究では、歯周病由来の特定の血清microRNAが、全身臓器のmRNAの遺伝子発現を制御し、病変を引き起こすという仮説を立て、候補となる血清microRNAを特定することを目的とした。
    ラットを用いて、歯周病を人工的に惹起させる群(実験群、8匹)と何もしない群(対照群、8匹)に分け4週間飼育した。まず肝臓に注目したところ、実験群でアポトーシスが認められた。また、2群間で、血清中の発現量が著しく異なるmicroRNA52種類と肝臓で発現量が著しく異なるmRNA33種類がスクリーニングされた。バイオインフォマティクス解析の結果、肝臓mRNAのうち、12種類(Gpr12、Hyou1、Rgma、Rad51、Dusp4、Chac1、Ier5、Ybx3、Pard6g、Bloc1s3、Sephs2、Il7)は、変動した血清miRNA (発現量比 >1.5、<0.67)の標的遺伝子と一致していることが判明した。そのうち、Hyou1、Chac1、Bloc1s3は、アポトーシスに関わる作用を有しており、これら3種類の肝臓mRNAの上流に存在する血清microRNAは、miR-3591、miR-181a-2-3p、miR-6321であった。
    以上のことから、歯周病モデルラットにおいて、miR-3591、miR-181a-2-3p、miR-6321は、肝臓mRNAであるHyou1、Chac1、Bloc1s3の遺伝子発現を制御し、肝細胞のアポトーシスを引き起こした可能性が示唆された。

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  • 乳酸菌の継続摂取による口腔細菌叢の質的量的分析と関連する唾液バイオマーカーの模索

    Grant number:21K10252  2021.04 - 2024.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    佐保 輝, 丸山 貴之, 森田 学, 江國 大輔

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    Grant amount:\4030000 ( Direct expense: \3100000 、 Indirect expense:\930000 )

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  • 次世代型アルツハイマー病モデルにおける酸化ストレス制御に着目した認知症予防の検討

    Grant number:21K10227  2021.04 - 2022.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    片岡 広太, 江國 大輔, 福原 大樹, 丸山 貴之, 中原 桃子, 福原 瑶子, 森田 学

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    Grant amount:\4030000 ( Direct expense: \3100000 、 Indirect expense:\930000 )

    アルツハイマー型認知症は加齢に伴って増加する神経変性疾患であり、症状の進行により最終的には寝たきり介護が必要な状態となる。脳内のアミロイドβの集積による神経毒性が疾患プロセスの中心とされているが、有効な治療法や、緩和方法が確立されていないのが現状である。神経変性の病態は未だ解明されていないが、アルツハイマー型認知症モデルマウスにおいて、抗酸化遺伝子の誘導が酸化ストレスと神経変性を抑制することが示された。近年、歯周病がマウスの脳細胞へのアミロイドβの蓄積量を増加させるがことが報告されている。歯周病の病態にも酸化ストレスが関与するが、歯周病に由来するアミロイドβの蓄積に酸化ストレスがどの程度関係するかは不明である。本研究では、アルツハイマー型認知症モデルマウスにおいて、歯周病由来のアミロイドβの蓄積に対する酸化ストレスの役割を検討して、抗酸化療法の予防効果について検討した。
    初年度は、最適なモデルを検討することとしていた。そこで、アルツハイマー型認知症モデルマウスを歯周病群と対照群に分けた。歯周病群には、下顎の第1臼歯に絹糸を巻き、歯周病を惹起した。対照群は無処置とした。2か月後に、マウスから脳を摘出した。一部は病理組織標本に用い、一部は遺伝子の抽出に用いた。対照群と比較して、歯周病群では脳内の酸化ストレスに関与する遺伝子が一部変動していた。脳内の2倍以上の変動があった316個の遺伝子群のうち、酸化ストレスに関与する遺伝子が2種類含まれていた。また、免疫染色の結果、海馬におけるアミロイドβの増加もみられた。

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  • がん化学療法に伴う歯周組織への影響に関する検討

    Grant number:20K10267  2020.04 - 2023.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    丸山 貴之, 森田 学, 江國 大輔, 小林 暉政, 杉浦 嘉雄

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    本研究の目的は、がん化学療法に用いられる5-フルオロウラシル(5-FU)投与による歯科疾患の重症化メカニズムについて、動物実験を行い、組織学的、生化学的な観点から解明することである。また、5-FU投与による歯周疾患の重症化に対する予防法についても検討する。
    2021年度は予備実験として、別研究(酸化ストレス可視化マウスによる抗がん剤の酸化ストレスの検討)で得られた血液および歯周組織を用いて、酸化ストレスの程度を比較検討した。マウスを3群(①コントロール群、②生理食塩水腹腔内投与群、③5-FU腹腔内投与群)に分類し、それぞれの処置を行った。1週間後、すべての群のマウスを多量のイソフルレンで安楽死させ、血液および歯周組織を採取した。その後、血清中および歯周組織中の酸化ストレスを測定し、比較検討した。
    その結果、5-FU腹腔内投与群はコントロール群、生理食塩水腹腔内投与群と比較して、血清中および歯周組織中の酸化ストレスが高い傾向を認めた。一方、コントロール群と生理食塩水腹腔内投与群の間で、血清中および歯周組織中の酸化ストレスに大きな違いは認められなかった。このことから、5-FU投与により、血清中の酸化ストレスが増加し、その酸化ストレスが歯周組織に影響を及ぼすことで、歯周状態を悪化する可能性が示唆された。

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  • gum chewing during postoperative fasting improve the prognosis after esophageal cancer surgery?

    Grant number:19K10444  2019.04 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    山中 玲子, 横井 彩, 森田 学, 丸山 貴之

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    本研究は,「胸部食道がん患者における周術期のガム咀嚼と舌圧との関連」を明らかにし、食道がん術後合併症予防に寄与する方策を歯科的に検討するものである。
    科研申請時は、食道がん手術を受ける患者を対象に,術後絶食期間中に,従来の口腔衛生管理のみを行う「対照群」と,術後絶食中にガム咀嚼による口腔機能訓練を加えて行う「介入群」にランダムに割付け,2群間で比較して,食道がん術後絶食中のガム咀嚼による咀嚼機能や嚥下機能の維持効果,予後改善効果を検討するランダム化比較試験(必要症例数約70例)としていた。その後、具体的な研究方法について、倫理委員会、各科の医師・歯科医師、パラメディカル、統計専門家等と議論し、過去の我々の研究でえた結果を対照群とし、周術期にガム咀嚼を行う1群のみの介入研究とすることとなった。
    我々の過去の研究では,食道がん術後2週間目に舌圧が低下した患者の割合は64.5%程度(38人/59人中)であったため,期待割合:40%,閾値割合:65%で計算すると,24症例で10人低下(41.7%)の場合,信頼区間は22.1%~63.3%で,両側p値=0.03,24症例で9人低下(37.5%)の場合,信頼区間は18.8%~59.4%で,両側p値=0.01となり,必要症例数は24例となった。また,過去の研究では,92名が参加して33名(35.9%)が同意撤回,中止,脱落したため,36%が脱落すると仮定し,その分も加味すると必要症例数は38例となる。今回,40例あれば,同意撤回,中止,脱落等が生じても,統計学的考察も可能と考えられるため,目標症例数は40例とした。
    令和3年度は、37症例を登録し、30症例が完了した。22症例を収集した時点で、中間解析を行ったところ、ガム咀嚼トレーニングを行った群では、有意に舌圧の低下を予防できたことを確認した。結果はがん口腔支持療法学会で発表した。
    令和4年度中に対象者の募集を終え、最終解析、学会発表、論文発表を行う予定である。

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  • Multifactorial analysis for progression of oral frail

    Grant number:17H04421  2017.04 - 2021.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Morita Manabu

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    Grant amount:\13130000 ( Direct expense: \10100000 、 Indirect expense:\3030000 )

    This clinical study determined the oral function which related to the physical frailty. A total of 203 outpatients of Preventive Dentistry Clinic at Okayama University Hospital participated. The path analysis, in the cross sectional study, revealed that ODK was positively associated with nutritional status. In addition, there was a negative association between nutritional status and the number of criterion met in physical frailty, and a positive association between nutritional status and walking distance without rest. In the cohort study, keeping good ODK was significantly associated mainly with confidence not falling, followed by the tongue pressure, masticatory function, swallowing function and number of present teeth. We concluded that articulatory oral motor skill, social participation and physical frailty were associated to each other in the aging process.

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  • Mechanism of periodontal disease on disease progression of non-alcoholic steatohepatitis

    Grant number:17K17371  2017.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    Maruyama Takayuki

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    Grant amount:\3640000 ( Direct expense: \2800000 、 Indirect expense:\840000 )

    Non-alcoholic hepatitis (NASH) can be a risk factor for periodontal disease, however, the mechanism that NASH exerts on the pathological progression of periodontal disease is unclear. On the other hand, microRNA in blood serves as a communication medium for systemic disease and periodontal disease. The purpose of this study was to clarify the influence of NASH on periodontal tissue by pairing analysis of microRNA in blood and gene expression in periodontal tissue. As a result, it is suggested that NASH regulates gene expression in periodontal tissue via microRNA in blood.

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  • Biomarker to reflect for condition of periodontitis

    Grant number:16K11855  2016.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Miyai Hisataka

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    The purpose of this study is to explore serum miRNAs which reflect periodontal condition and which can influence systematic condition. Wistar rats aged 8-weeks old (n=16) were divided into two groups. Cotton ligature was placed in a submarginal position on the maxillary second molars for 4 weeks to induce periodontitis in periodontitis group. Control groups received no treatment for 4 weeks. Ligation around teeth induced periodontal inflammation and periodontal tissue destruction. In serum investigation, microarray and real-time PCR results showed that miR-376b-3p could be biomarker for periodontitis. Microarray of liver and integrated analysis between serum miR-376b and mRNA of liver was examined. Periodontitis could affect the liver through serum miRNAs.

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  • Relation between oral health status and pancreatobiliary tract cancer

    Grant number:26670904  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    Morita Manabu

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    Grant amount:\3640000 ( Direct expense: \2800000 、 Indirect expense:\840000 )

    Patients diagnosed with pancreatobiliary tract cancer were analyzed. Factors affecting the prognosis of pancreatobiliary tract cancer were evaluated. The results demonstrated that low body mass index, high concentration of serum C-reactive protein (CRP) and severe periodontitis were significant prognostic factors for survival rate. It was also found that micro-RNAs in salivary exosomes could be candidate biomarkers for pancreatobiliary tract cancer.

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  • Topical application of ointment containing 0.5% green tea catechins suppresses tongue oxidative stress in 5-fluorouracil administered rats

    Grant number:26861830  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    Maruyama Takayuki

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    Grant amount:\3640000 ( Direct expense: \2800000 、 Indirect expense:\840000 )

    The purpose of this study was to investigate the preventive effects of topical application of catechins on tongue oxidative stress induced by 5-FU administration in rats. Rats were divided into four groups of seven rats each: a negative control group (saline + ointment without catechins), a positive control group (5-FU + ointment without catechins), and two experimental groups (5-FU + ointment containing 0.1% or 0.5% catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-OHdG was determined to evaluate oxidative stress.
    The ratios of 8-OHdG positive cells were higher in the positive control group than in the negative control group. On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group.
    Topical application of ointment containing 0.5% catechins could prevent tongue oxidative stress in 5-FU administered rats.

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  • The effectiveness of postoperative continuous oral management in perioperative care

    Grant number:25463242  2013.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Mizutani Shinsuke, MORITA Manabu, TOMOFUJI Takaaki, EKUNI Daisuke, MARUYAMA Takayuki, YOKOI Aya, TANIGUCHI Ayano, MIZUNO Hirofumi

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    The purpose of this study was to determine the effect of professional oral healthcare on postoperative complications after esophagectomy among patients with esophageal cancer.
    Patients were divided into two groups: patients who received professional oral healthcare from dentists after surgery (oral care group, n = 21) and those who did not receive oral healthcare (control group, n = 23).
    Logistic regression analysis showed that the risk for postoperative pyrexia (≧38.0℃) was associated with the lack of professional oral healthcare intervention (Odds ratio: 13.09; 95%confidence interval: 2.04-84.03; P < 0.05) and anastomotic leakage (Odds ratio: 28.15; 95%confidence interval: 2.06-384.25; P < 0.05). This suggests that professional oral healthcare intervention is effective in reducing the incidence of postoperative pyrexia in patients who undergo esophagectomy.

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  • Prevention of cancer by oral care inhibiting the production of acetaldehyde

    Grant number:24593154  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    YOKOI AYA, YAMANAKA Reiko, MORITA Manabu, YAMASAKI Yutaka, KASHIWAZAKI Haruhiko, HATA Hironobu, TOMOFUJI Takaaki, TAMAKI Naofumi, EKUNI Daisuke, MARUYAMA Takayuki, SOGA Yoshihiko

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    Grant amount:\5070000 ( Direct expense: \3900000 、 Indirect expense:\1170000 )

    The aim of this study was to investigate the relationship between acetaldehyde concentration in mouth air and tongue coating volume in healthy volunteers. Acetaldehyde concentration in participants with a tongue coating score of 3 was significantly higher than those with a score of 1. After removing tongue coating, acetaldehyde concentration was significantly decreased.
    Oral microflora on the tongue may be the main source of local production of acetaldehyde. Removing tongue coating may be a method inhibiting the production of acetaldehyde.

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  • Effectiveness of comprehensive food education (Shokuiku) against the lifestyle-related disease

    Grant number:23593089  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    MARUYAMA Takayuki, MORITA Manabu, TOMOFUJI Takaaki, EKUNI Daisuke, YAMANAKA Reiko, TAKEUCHI Noriko

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    Grant amount:\4940000 ( Direct expense: \3800000 、 Indirect expense:\1140000 )

    Recently, it is noticed the importance of comprehensive food education (Shokuiku) against the lifestyle-related disease. In this study, we investigated whether eating until full, eating quickly, or both eating behaviors were associated with being overweight, the relationship between subjective and objective methods to evaluate eating quickly and to numerically characterize the kinesis of eating quickly. Also, we investigated the effects of intervention using a pamphlet or the combination of a pamphlet and dietary records on improvement of eating quickly. Furthermore, we investigated the relationship between knowledge about comprehensive food education and dental caries experience.

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Class subject in charge

  • Human Ecology (2023academic year) Fourth semester  - 火3

  • Health Statistics (2023academic year) Second semester  - 水3

  • Public Health (2023academic year) Fourth semester  - 火1,火2

  • Practice in Oral Health Evaluation (2023academic year) 1st semester  - 月5,月6,月7

  • Oral Health (2023academic year) Third semester  - 金2,金3

  • Dental Health (2023academic year) 1st and 2nd semester  - 金7~8

  • Etiology of Oral Diseases (2023academic year) 1st semester  - 月4

  • Epidemiology (2023academic year) Second semester  - 水1,水2

  • Social Dentistry & Forensic Dentistry (2023academic year) Third semester  - 水4,水5

  • Clinical Preventive Dentistry (2023academic year) Second semester  - 月1,月2

  • Human Ecology (2022academic year) Fourth semester  - 火3

  • Health Statistics (2022academic year) Second semester  - 水3

  • Public Health (2022academic year) Fourth semester  - 火1,火2

  • Practice in Oral Health Evaluation (2022academic year) 1st semester  - 月5,月6,月7

  • Oral Health (2022academic year) Third semester  - 金2,金3

  • Etiology of Oral Diseases (2022academic year) 1st semester  - 月4

  • Epidemiology (2022academic year) Second semester  - 水1,水2

  • Social Dentistry &? Forensic Dentistry (2022academic year) Third semester  - 水4,水5

  • Clinical Preventive Dentistry (2022academic year) Second semester  - 月1,月2

  • Practice in Oral Health Evaluation (2021academic year) 1st semester  - 月5,月6,月7

  • Etiology of Oral Diseases (2021academic year) 1st semester  - 月4

  • Practice in Oral Health Evaluation (2020academic year) 1st semester  - 月5,月6,月7

  • Etiology of Oral Diseases (2020academic year) 1st semester  - 月4

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Media Coverage

  • 肥満になると歯周病悪化 Newspaper, magazine

    山陽新聞  2022.7.4

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    Author:Other 

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  • 歯周病 やはり肥満が大敵 動物実験でメカニズム解明 Newspaper, magazine

    朝日新聞  2022.5.28

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    Author:Other 

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