Updated on 2022/10/01

写真a

 
HAYANO Satoru
 
Organization
Okayama University Hospital Lecturer
Position
Lecturer
External link

Degree

  • Doctor (Dentistry) ( Okayama University )

Research Interests

  • Craniofacial development

  • 頭蓋顔面発生

Research Areas

  • Life Science / Developmental dentistry

Education

  • Okayama University    

    - 2011

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  • Okayama University   医歯薬学総合研究科  

    - 2011

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    Country: Japan

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  • Okayama University    

    - 2006

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  • Okayama University   歯学部   歯学科

    - 2006

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    Country: Japan

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Research History

  • Okayama University Hospital   Department of Orthodontics   Lecturer

    2019.2

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    Country:Japan

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  • Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences   Department of Orthodontics   Assistant Professor

    2015.3 - 2019.1

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  • University of Michigan School of Dentistry   Department of Biologic and Materials Sciences   Research Fellow

    2013.1 - 2015.2

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  • Okayama University   Department of Orthodontics Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences   Assistant Professor

    2012.6 - 2012.12

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  • - Assistant Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2012

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Professional Memberships

 

Papers

  • Role of the inferior alveolar nerve in rodent lower incisor stem cells

    Hayano S, Fukui Y, Kawanabe N, Kono K, Nakamura M, Ishihara Y, Kamioka H

    Journal of Dental Research   97 ( 8 )   954 - 961   2018

  • Investigation of the molecular causes underlying physical abnormalities in Diamond‐Blackfan anemia patients with RPL5 haploinsufficiency Reviewed

    Yuko Fukui, Satoru Hayano, Noriaki Kawanabe, Ziyi Wang, Akira Shimada, Megumu K. Saito, Isao Asaka, Hiroshi Kamioka

    Pathology International   71 ( 12 )   803 - 813   2021.9

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/pin.13168

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pin.13168

  • O‐GlcNAcylation drives calcium signaling toward osteoblast differentiation: A bioinformatics‐oriented study Reviewed

    Yao Weng, Ziyi Wang, Yoko Fukuhara, Airi Tanai, Mika Ikegame, Daisuke Yamada, Takeshi Takarada, Takashi Izawa, Satoru Hayano, Kaya Yoshida, Hiroshi Kamioka, Hirohiko Okamura

    BioFactors   47 ( 6 )   992 - 1015   2021.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/biof.1774

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/biof.1774

  • A case of Angle Class II malocclusion with severe gummy smile and deep overbite treated with temporary anchorage devices for maxillary incisors intrusion Reviewed

    HAYANO Satoru, SUMIYOSHI-MIYAMOTO Kumi, KAMIOKA Hiroshi

    32 ( 1 )   17 - 24   2020.8

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

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  • Mechanical regulation of bone homeostasis through p130Cas-mediated alleviation of NF-κB activity Reviewed International journal

    T. Miyazaki, Z. Zhao, Y. Ichihara, D. Yoshino, T. Imamura, K. Sawada, S. Hayano, H. Kamioka, S. Mori, H. Hirata, K. Araki, K. Kawauchi, K. Shigemoto, S. Tanaka, L. F. Bonewald, H. Honda, M. Shinohara, M. Nagao, T. Ogata, I. Harada, Y. Sawada

    Science Advances   5 ( 9 )   eaau7802   2019.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Association for the Advancement of Science ({AAAS})  

    Mechanical loading plays an important role in bone homeostasis. However, molecular mechanisms behind the mechanical regulation of bone homeostasis are poorly understood. We previously reported p130Cas (Cas) as a key molecule in cellular mechanosensing at focal adhesions. Here, we demonstrate that Cas is distributed in the nucleus and supports mechanical loading-mediated bone homeostasis by alleviating NF-κB activity, which would otherwise prompt inflammatory processes. Mechanical unloading modulates Cas distribution and NF-κB activity in osteocytes, the mechanosensory cells in bones. Cas deficiency in osteocytes increases osteoclastic bone resorption associated with NF-κB-mediated RANKL expression, leading to osteopenia. Upon shear stress application on cultured osteocytes, Cas translocates into the nucleus and down-regulates NF-κB activity. Collectively, fluid shear stress-dependent Cas-mediated alleviation of NF-κB activity supports bone homeostasis. Given the ubiquitous expression of Cas and NF-κB together with systemic distribution of interstitial fluid, the Cas-NF-κB interplay may also underpin regulatory mechanisms in other tissues and organs.

    DOI: 10.1126/sciadv.aau7802

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  • Compound mutations in Bmpr1a and Tak1 synergize facial deformities via increased cell death

    Xia Liu, Satoru Hayano, Haichun Pan, Maiko Inagaki, Jun Ninomiya-Tsuji, Hongchen Sun, Yuji Mishina

    Genesis   56 ( 3 )   2018.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:John Wiley and Sons Inc.  

    BMP signaling plays a critical role in craniofacial development. Augmentation of BMPR1A signaling through neural crest-specific expression of constitutively active Bmpr1a (caBmpr1a) results in craniofacial deformities in mice. To investigate whether deletion of Tak1 may rescue the craniofacial deformities caused by enhanced Smad-dependent signaling through caBMPR1A, we generated embryos to activate transcription of caBmpr1a transgene and ablate Tak1 in neural crest derivatives at the same time. We found that deformities of the double mutant mice showed more severe than those with each single mutation, including median facial cleft and cleft palate. We found higher levels of cell death in the medial nasal and the lateral nasal processes at E10.5 in association with higher levels of p53 in the double mutant embryos. We also found higher levels of pSmad1/5/9 in the lateral nasal processes at E10.5 in the double mutant embryos. Western analyses revealed that double mutant embryos showed similar degrees of upregulation of pSmad1/5/9 with caBmpr1a or Tak1-cKO embryos while the double mutant embryos showed higher levels of phospho-p38 than caBmpr1a or Tak1-cKO embryos at E17.5, but not at E10.5. It suggested that deletion of Tak1 aggravates the craniofacial deformities of the caBmpr1a mutants by increasing p53 and phospho-p38 at different stage of embryogenesis.

    DOI: 10.1002/dvg.23093

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  • 岡山大学大学院歯科矯正学分野研究室における過去15年間および2013年から2016年の4年間の岡山大学の学生および職員に対する矯正相談の実態調査 Reviewed

    田中 智代, 村上 隆, 片岡 伴記, 石原 嘉人, 植田 紘貴, 早野 暁, 星島 光博, 中村 政裕, 川邉 紀章, 上岡 寛

    岡山歯学会雑誌   36 ( 2 )   45 - 51   2018.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

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  • Role of Osteocyte-PDL Crosstalk in Tooth Movement via SOST/Sclerostin

    Odagaki N, Ishihara Y, Wang Z, Ei Hsu, Hlaing E, Nakamura M, Hoshijima M, Hayano S, Kawanabe N, Kamioka H

    Journal of Dental Research   2018

  • Rapamycin rescues BMP mediated midline craniosynostosis phenotype through reduction of mTOR signaling in a mouse model

    Kramer K, Yang J, Swanson WB, Hayano S, Toda M, Pan H, Kim JK, Krebsbach PH, Mishina Y

    Genesis   56 ( 6 )   2018

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  • Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model

    Mitsuaki Ono, Masamitsu Oshima, Miho Ogawa, Wataru Sonoyama, Emilio Satoshi Hara, Yasutaka Oida, Shigehiko Shinkawa, Ryu Nakajima, Atsushi Mine, Satoru Hayano, Satoshi Fukumoto, Shohei Kasugai, Akira Yamaguchi, Takashi Tsuji, Takuo Kuboki

    Scientific Reports   7   44522 - 44522   2017.3

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Nature  

    DOI: 10.1038/srep44522

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  • 岡山大学歯科矯正学分野「歯の移動の臨床手技実習」における60分授業・クォーター制への対応

    片岡伴記, 星島光博, 原規子, 中村政裕, 早野暁, 村上隆, 川邊紀章, 上岡寛

    岡山歯学会雑誌   35 ( 2 )   59 - 65   2017

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    Language:Japanese   Publisher:岡山歯学会  

    当大学の歯科矯正学分野において、「歯の移動の臨床手技実習」における60分授業・クォーター制を導入し、導入前後の年度で学生と教職員にアンケートを実施した。学生アンケートでは、導入前の平成27年度の実習受講生53名と導入後の平成28年度の受講生46名を対象とした。教職員アンケートでは、平成28年度の実習で指導にあたった教職員7名と補助した医局員20名を対象とした。その結果、導入後では実習内容、指導法、実習書といった教育コンテンツの改善により学生の自修意欲が高まり、一定の成果を上げることができていた。また、ICTを活用した授業支援システム"WebClass"も予習・復習に積極的に利用されており、成果に貢献していた。しかし、実習時間の短縮への対応の点では問題が残り、「時間不足」を訴える意見が多く聞かれた。

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  • Alternation in the gap junctional intercellular communication capacity during the maturation of osteocytes in the embryonic chick calvaria

    Ziyi Wang, Naoya Odagaki, Tomoyo Tanaka, Mana Hashimoto, Masahiro Nakamura, Satoru Hayano, Yoshihito Ishihara, Noriaki Kawanabe, Hiroshi Kamioka

    BONE   91   20 - 29   2016.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE INC  

    Introduction: The intercellular network of cell-cell communication among osteocytes is mediated by gap junctions. Gap junctional intercellular communication (GJIC) is thought to play an important role in the integration and synchronization of bone remodeling. To further understand the mechanism of bone development it is important to quantify the difference in the GJIC capacity of young and developmentally mature osteocytes.
    Materials and methods: We first established an embryonic chick calvaria growth model to show the growth of the calvaria in embryos at 13 to 21 days of age. We then applied a fluorescence recovery after photobleaching (FRAP) technique to compare the difference in the GJIC capacity of young osteocytes with that of developmentally mature osteocytes. Finally, we quantified the dye (Calcein) diffusion from the FRAP data using a mathematic model of simple diffusion which was also used to identify simple diffusion GJIC pattern cells (fitted model) and accelerated diffusion GJIC pattern cells (non-fitted model).
    Results: The relationship between the longest medial-lateral length of the calvaria (frontal bone) and the embryonic age fit a logarithmic growth model: length = 5.144 x ln(day) - 11.340. The morphometric data during osteocyte differentiation showed that the cellular body becomes more spindle-shaped and that the cell body volume decreased by approximately 22% with an increase in the length of the processes between the cells. However, there were no significant differences in the cellular body surface area or in the distance between the mass centres of the cells. The dye-displacement rate in young osteocytes was significantly higher than that in developmentally mature osteocytes: dye displacement only occurred in 26.88% of the developmentally mature osteocytes, while it occurred in 64.38% of the young osteocytes. Additionally, in all recovered osteocytes, 36% of the developmentally mature osteocytes comprised non-fitted model cells while 53.19% of the young osteocytes were the non-fitted model, which indicates the active transduction of dye molecules. However, there were no statistically significant differences between the young and developmentally mature osteocytes with regard to the diffusion coefficient, permeability coefficient, or permeance of the osteocyte processes, which were 3.93 +/- 3.77 (x 10(-8) cm(2)/s), 5.12 +/- 4.56 (x 10(-8) cm(2)/s) and 2.99 +/- 2.47 (x 10(-13) cm(2)/s) (mean SD), respectively.
    Conclusions: These experiments comprehensively quantified the GJIC capacity in the embryonic chick calvaria and indicated that the cell-cell communication capacity of the osteocytes in the embryonic chick calvaria was related to their development. (C) 2016 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bone.2016.06.016

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  • Common mechanisms in development and disease: BMP signaling in craniofacial development

    Daniel Graf, Zeba Malik, Satoru Hayano, Yuji Mishina

    CYTOKINE & GROWTH FACTOR REVIEWS   27   129 - 139   2016.2

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    BMP signaling is one of the key pathways regulating craniofacial development. It is involved in the early patterning of the head, the development of cranial neural crest cells, and facial patterning. It regulates development of its mineralized structures, such as cranial bones, maxilla, mandible, palate, and teeth. Targeted mutations in the mouse have been instrumental to delineate the functional involvement of this signaling network in different aspects of craniofacial development. Gene polymorphisms and mutations in BMP pathway genes have been associated with various non-syndromic and syndromic human craniofacial malformations. The identification of intricate cellular interactions and underlying molecular pathways illustrate the importance of local fine-regulation of Bmp signaling to control proliferation, apoptosis, epithelial-mesenchymal interactions, and stem/progenitor differentiation during craniofacial development. Thus, BMP signaling contributes both to shape and functionality of our facial features. BMP signaling also regulates postnatal craniofacial growth and is associated with dental structures life-long. A more detailed understanding of BMP function in growth, homeostasis, and repair of postnatal craniofacial tissues will contribute to our ability to rationally manipulate this signaling network in the context of tissue engineering. (C) 2015 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.cytogfr.2015.11.004

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  • 歯科矯正用アンカースクリューおよびオクルーザルスプリントを 用いて咬合再構築を行った成人開咬症例 Reviewed

    早野暁, 星島光博, 石川崇典, 上岡寛

    中・四矯歯誌   28 ( 1 )   1 - 9   2016

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  • BMP signaling mediated by constitutively active Activin type 1 receptor (ACVR1) results in ectopic bone formation localized to distal extremity joints

    Shailesh Agarwal, Shawn J. Loder, Cameron Brownley, Oluwatobi Eboda, Jonathan R. Peterson, Satoru Hayano, Bingrou Wu, Bin Zhao, Vesa Kaartinen, Victor C. Wong, Yuji Mishina, Benjamin Levi

    DEVELOPMENTAL BIOLOGY   400 ( 2 )   202 - 209   2015.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    BMP signaling mediated by ACVR1 plays a critical role for development of multiple structures including the cardiovascular and skeletal systems. While deficient ACVR1 signaling impairs normal embryonic development, hyperactive ACVR1 function (R206H in humans and Q207D mutation in mice, ca-ACVR1) results in formation of heterotopic ossification (HO). We developed a mouse line, which conditionally expresses ca-ACVR1 with Nfatcl-Cre transgene. Mutant mice developed ectopic cartilage and bone at the distal joints of the extremities including the interphalangeal joints and hind limb ankles as early as P4 in the absence of trauma or exogenous bone morphogenetic protein (BMP) administration. Micro-CT showed that even at later time points (up to P40), cartilage and bone development persisted at the affected joints most prominently in the ankle. Interestingly, this phenotype was not present in areas of bone outside of the joints - tibia are normal in mutants and littermate controls away from the ankle. These findings demonstrate that this model may allow for further studies of heterotopic ossification, which does not require the use of stem cells, direct trauma or activation with exogenous Cre gene administration. (C) 2015 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.ydbio.2015.02.011

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  • Augmented BMP signaling in the neural crest inhibits nasal cartilage morphogenesis by inducing p53-mediated apoptosis

    Satoru Hayano, Yoshihiro Komatsu, Haichun Pan, Yuji Mishina

    DEVELOPMENT   142 ( 7 )   1357 - 1367   2015.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:COMPANY OF BIOLOGISTS LTD  

    Bone morphogenetic protein (BMP) signaling plays many roles in skull morphogenesis. We have previously reported that enhanced BMP signaling through the BMP type IA receptor (BMPR1A) in cranial neural crest cells causes craniosynostosis during postnatal development. Additionally, we observed that 55% of Bmpr1a mutant mice show neonatal lethality characterized by a distended gastrointestinal tract. Here, we show that severely affected mutants exhibit defective nasal cartilage, failure of fusion between the nasal septum and the secondary palate, and higher levels of phosphorylated SMAD1 and SMAD5 in the nasal tissue. TUNEL demonstrated an increase in apoptosis in both condensing mesenchymal tissues and cartilage of the nasal region in mutants. The levels of p53 (TRP53) tumor suppressor protein were also increased in the same tissue. Injection of pifithrin-alpha, a chemical inhibitor of p53, into pregnant mice prevented neonatal lethality while concomitantly reducing apoptosis in nasal cartilage primordia, suggesting that enhanced BMP signaling induces p53-mediated apoptosis in the nasal cartilage. The expression of Bax and caspase 3, downstream targets of p53, was increased in the mutants; however, the p53 expression level was unchanged. It has been reported that MDM2 interacts with p53 to promote degradation. We found that the amount of MDM2-p53 complex was decreased in all mutants, and the most severely affected mutants had the largest decrease. Our previous finding that the BMP signaling component SMAD1 prevents MDM2-mediated p53 degradation coupled with our new data indicate that augmented BMP signaling induces p53-mediated apoptosis by prevention of p53 degradation in developing nasal cartilage. Thus, an appropriate level of BMP signaling is required for proper craniofacial morphogenesis.

    DOI: 10.1242/dev.118802

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  • Runx/Cbfb signaling regulates postnatal development of granular convoluted tubule in the mouse submandibular gland. International journal

    Md Nurul Islam, Shinsuke Itoh, Takeshi Yanagita, Kumi Sumiyoshi, Satoru Hayano, Koh-Ichi Kuremoto, Hiroshi Kurosaka, Tadashi Honjo, Noriaki Kawanabe, Hiroshi Kamioka, Takayoshi Sakai, Naozumi Ishimaru, Ichiro Taniuchi, Takashi Yamashiro

    Developmental dynamics : an official publication of the American Association of Anatomists   244 ( 3 )   488 - 96   2015.3

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    BACKGROUND: The rodent salivary gland is not fully developed at birth and the cellular definitive differentiation takes place postnatally. However, little is known about its molecular mechanism. RESULTS: Here we provide the loss-of-function genetic evidence that Runx signaling affects postnatal development of the submandibular gland (SMG). Core binding factor β (Cbfb) is a cotranscription factor which forms a heterodimer with Runx proteins. Cbfb was specifically expressed in the duct epithelium, specifically in the SMG. Epithelial Cbfb deficiency resulted in decrease in the size of the SMG and in the saliva secretion on postnatal day 35. The Cbfb mutant SMG specifically exhibited involution of the granular convoluted tubules (GCT), with a down-regulated expression of its marker genes, such as Klk1, Ngf, and Egf. The induction of GCT is under the control of androgens, and the Cbfb mutant SMG demonstrated down-regulated expression of Crisp3, an androgen-dependent transcript. Because the circulating testosterone or tissue dihydrotestosterone levels were not affected in the Cbfb mutants, it appears that Runx/Cbfb signaling regulate androgen receptor pathway, but does not affect the circulating testosterone levels or the enzymatic conversion to DHT. CONCLUSIONS: Runx signaling is important in the postnatal development of androgen-dependent GCT in the SMG.

    DOI: 10.1002/dvdy.24231

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  • Topical Application of Lithium Chloride on the Pulp Induces Dentin Regeneration

    Kazuya Ishimoto, Satoru Hayano, Takeshi Yanagita, Hiroshi Kurosaka, Noriaki Kawanabe, Shinsuke Itoh, Mitsuaki Ono, Takuo Kuboki, Hiroshi Kamioka, Takashi Yamashiro

    PLOS ONE   10 ( 3 )   2015.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

    We herein describe a novel procedure for dentin regeneration that mimics the biological processes of tooth development in nature. The canonical Wnt signaling pathway is an important regulator of the Dentin sialophosphoprotein (Dspp) expression. Our approach mimics the biological processes underlying tooth development in nature and focuses on the activation of canonical Wnt signaling to trigger the natural process of dentinogenesis. The coronal portion of the dentin and the underlying pulp was removed from the first molars. We applied lithium chloride (LiCl), an activator of canonical Wnt signaling, on the amputated pulp surface to achieve transdifferentiation toward odontoblasts from the surrounding pulpal cells. MicroCT and microscopic analyses demonstrated that the topical application of LiCl induced dentin repair, including the formation of a complete dentin bridge. LiCl-induced dentin is a tubular dentin in which the pulp cells are not embedded within the matrix, as in primary dentin. In contrast, a dentin bridge was not induced in the control group treated with pulp capping with material carriers alone, although osteodentin without tubular formation was induced at a comparatively deeper position from the pulp exposure site. We also evaluated the influence of LiCl on differentiation toward odontoblasts in vitro. In the mDP odontoblast cell line, LiCl activated the mRNA expression of Dspp, Axin2 and Kallikrein 4 (Klk4) and downregulated the Osteopontin (Osp) expression. These results provide a scientific basis for the biomimetic regeneration of dentin using LiCl as a new capping material to activate dentine regeneration.

    DOI: 10.1371/journal.pone.0121938

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  • Ex vivo real-time observation of Ca2+ signaling in living bone in response to shear stress applied on the bone surface

    Yoshihito Ishihara, Yasuyo Sugawara, Hiroshi Kamioka, Noriaki Kawanabe, Satoru Hayano, Tarek A. Balam, Keiji Naruse, Takashi Yamashiro

    BONE   53 ( 1 )   204 - 215   2013.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE INC  

    Bone cells respond to mechanical stimuli by producing a variety of biological signals, and one of the earliest events is intracellular calcium ([Ca2+](i)) mobilization. Our recently developed ex vivo live [Ca2+](i) imaging system revealed that bone cells in intact bone explants showed autonomous [Ca2+](i) oscillations, and osteocytes specifically modulated these oscillations through gap junctions. However, the behavior and connectivity of the [Ca2+](i) signaling networks in mechanotransduction have not been investigated in intact bone. We herein introduce a novel fluid-flow platform for probing cellular signaling networks in live intact bone, which allows the application of capillary-driven flow just on the bone explant surface while performing real-time fluorogenic monitoring of the [Ca2+](i) changes. In response to the flow, the percentage of responsive cells was increased in both osteoblasts and osteocytes, together with upregulation of c-fos expression in the explants. However, enhancement of the peak relative fluorescence intensity was not evident. Treatment with 18 alpha-GA, a reversible inhibitor of gap junction, significantly blocked the [Ca2+](i), responsiveness in osteocytes without exerting any major effect in osteoblasts. On the contrary, such treatment significantly decreased the flow-activated oscillatory response frequency in both osteoblasts and osteocytes. The stretch-activated membrane channel, when blocked by Gd3+, is less affected in the flow-induced [Ca2+](i) response. These findings indicated that flow-induced mechanical stimuli accompanied the activation of the autonomous [Ca2+](i) oscillations in both osteoblasts and osteocytes via gap junction-mediated cell-cell communication and hemichannel. Although how the bone sense the mechanical stimuli in vivo still needs to be elucidated, the present study suggests that cell-cell signaling via augmented gap junction and hemichannel-mediated [Ca2+](i) mobilization could be involved as an early signaling event in mechanotransduction. (C) 2012 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bone.2012.12.002

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  • Surgically assisted rapid palatal expansion(SARPE)を併用した骨格性下顎前突症例

    早野 暁, 菅原 康代, 山城 隆

    中・四国矯正歯科学会雑誌   24 ( 1 )   113 - 113   2012.8

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  • Surgically assisted rapid palatal expansion(SARPE)を併用した骨格性下顎前突症例

    早野 暁, 菅原 康代, 吉岡 徳枝, 西山 明慶, 佐々木 朗, 山城 隆

    日本顎変形症学会雑誌   22 ( 2 )   178 - 178   2012.5

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    Language:Japanese   Publisher:(NPO)日本顎変形症学会  

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  • Core binding factor beta functions in the maintenance of stem cells and orchestrates continuous proliferation and differentiation in mouse incisors. Reviewed

    Kurosaka H, Islam MN, Kuremoto K, Hayano S, Nakamura M, Kawanabe N, Yanagita T, Rice DP, Harada H, Taniuchi I, Yamashiro T

    Stem cells (Dayton, Ohio)   29 ( 11 )   1792 - 1803   2011.11

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    Rodent incisors grow continuously throughout life, and epithelial progenitor cells are supplied from stem cells in the cervical loop. We report that epithelial Runx genes are involved in the maintenance of epithelial stem cells and their subsequent continuous differentiation and therefore growth of the incisors. Core binding factor β (Cbfb) acts as a binding partner for all Runx proteins, and targeted inactivation of this molecule abrogates the activity of all Runx complexes. Mice deficient in epithelial Cbfb produce short incisors and display marked underdevelopment of the cervical loop and suppressed epithelial Fgf9 expression and mesenchymal Fgf3 and Fgf10 expression in the cervical loop. In culture, FGF9 protein rescues these phenotypes. These findings indicate that epithelial Runx functions to maintain epithelial stem cells and that Fgf9 may be a target gene of Runx signaling. Cbfb mutants also lack enamel formation and display downregulated Shh mRNA expression in cells differentiating into ameloblasts. Furthermore, Fgf9 deficiency results in a proximal shift of the Shh expressing cell population and ectopic FGF9 protein suppresses Shh expression. These findings indicate that Shh as well as Fgf9 expression is maintained by Runx/Cbfb but that Fgf9 antagonizes Shh expression. The present results provide the first genetic evidence that Runx/Cbfb genes function in the maintenance of stem cells in developing incisors by activating Fgf signaling loops between the epithelium and mesenchyme. In addition, Runx genes also orchestrate continuous proliferation and differentiation by maintaining the expression of Fgf9 and Shh mRNA. © AlphaMed Press.

    DOI: 10.1002/stem.722

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  • チタンスクリューを用いて下顎大臼歯の圧下および遠心移動を行った開咬症例

    本城 正, 早野 暁, 上岡 寛, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集   70回   339 - 339   2011.10

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  • 歯の発生及びエナメル芽細胞分化におけるcore-binding factor β(Cbfb)の役割について

    黒坂 寛, 早野 暁, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集   69回   187 - 187   2010.9

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  • ヘパラン硫酸の糖残基修飾は象牙質形成に関与する

    早野 暁, 黒坂 寛, 富田 奈緒, 柳田 剛, 川邊 紀章, ディルクス・トーマス, カルス・イナ, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集   69回   185 - 185   2010.9

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  • Isolation of multipotent stem cells in human periodontal ligament using stage-specific embryonic antigen-4. Reviewed

    Kawanabe N, Murata S, Murakami K, Ishihara Y, Hayano S, Kurosaka H, Kamioka H, Takano-Yamamoto T, Yamashiro T

    Differentiation; research in biological diversity   79 ( 2 )   74 - 83   2010.2

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    The periodontal ligament (PDL) comprises adult stem cells, which are responsible for periodontal tissue regeneration. In the present study, we investigated the specific profile of the stem cells in the human PDL. Microscopic analysis demonstrated that PDL cells showed a fibroblastic appearance, forming flat and loose aggregates. PDL cells expressed embryonic stem cell-associated antigens (SSEA-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, OCT4, NANOG, SOX2, and REX1, and alkaline phosphatase activity), as well as conventional mesenchymal stem cell markers. When PDL cells were cultured in the presence of all-trans-retinoic acid, the numbers of SSEA-3+ and SSEA-4+ PDL cells were significantly decreased, while that of SSEA-1+ was increased. SSEA-4+ PDL cells showed a greater telomere length and growth rate. SSEA-4+ PDL cells exhibited the potential to generate specialized cells derived from three embryonic germ layers: mesodermal (adipocytes, osteoblasts, and chondrocytes), ectodermal (neurons), and endodermal (hepatocytes) lineages. Our findings demonstrated that SSEA-4, a major antigen to distinguish human embryonic stem cells, could also be used to identify multipotent stem cells in the PDL. Hence, SSEA-4+ human PDL cells appear to be a promising source of stem cells for regenerative medicine. © 2009 International Society of Differentiation.

    DOI: 10.1016/j.diff.2009.10.005

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MISC

  • 象牙質形成におけるヘパラン硫酸の糖残基修飾とWnt10aの役割について

    早野 暁, 黒坂 寛, 柳田 剛志, 川邉 紀章, カルス・イナ, ディルクス・トーマス, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集   71回   175 - 175   2012.9

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  • Roles of Heparan Sulfate Sulfation in Dentinogenesis

    Satoru Hayano, Hiroshi Kurosaka, Takeshi Yanagita, Ina Kalus, Fabian Milz, Yoshihito Ishihara, Md. Nurul Islam, Noriaki Kawanabe, Masahiro Saito, Hiroshi Kamioka, Taiji Adachi, Thomas Dierks, Takashi Yamashiro

    JOURNAL OF BIOLOGICAL CHEMISTRY   287 ( 15 )   12217 - 12229   2012.4

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    Language:English   Publisher:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Cell surface heparan sulfate (HS) is an essential regulator of cell signaling and development. HS traps signaling molecules, like Wnt in the glycosaminoglycan side chains of HS proteoglycans (HSPGs), and regulates their functions. Endosulfatases Sulf1 and Sulf2 are secreted at the cell surface to selectively remove 6-O-sulfate groups from HSPGs, thereby modifying the affinity of cell surface HSPGs for its ligands. This study provides molecular evidence for the functional roles of HSPG sulfation and desulfation in dentinogenesis. We show that odontogenic cells are highly sulfated on the cell surface and become desulfated during their differentiation to odontoblasts, which produce tooth dentin. Sulf1/Sulf2 double null mutant mice exhibit a thin dentin matrix and short roots combined with reduced expression of dentin sialophosphoprotein (Dspp) mRNA, encoding a dentin-specific extracellular matrix precursor protein, whereas single Sulf mutants do not show such defective phenotypes. In odontoblast cell lines, Dspp mRNA expression is potentiated by the activation of the Wnt canonical signaling pathway. In addition, pharmacological interference with HS sulfation promotes Dspp mRNA expression through activation of Wnt signaling. On the contrary, the silencing of Sulf suppresses the Wnt signaling pathway and subsequently Dspp mRNA expression. We also show that Wnt10a protein binds to cell surface HSPGs in odontoblasts, and interference with HS sulfation decreases the binding affinity of Wnt10a for HSPGs, which facilitates the binding of Wnt10a to its receptor and potentiates the Wnt signaling pathway, thereby up-regulating Dspp mRNA expression. These results demonstrate that Sulf-mediated desulfation of cellular HSPGs is an important modification that is critical for the activation of the Wnt signaling in odontoblasts and for production of the dentin matrix.

    DOI: 10.1074/jbc.M111.332924

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  • 象牙質形成におけるWntシグナリングとヘパラン硫酸の糖残基修飾

    早野 暁, 黒坂 寛, 冨田 奈緒, 柳田 剛志, 川邉 紀章, ディルクス・トーマス, カルス・イナ, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集   70回   250 - 250   2011.10

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  • 歯の発生及びエナメル芽細胞分化におけるcore-binding factorβ(Cbfb)の役割について

    黒坂 寛, 早野 暁, 呉本 晃一, 原田 英光, 山城 隆

    Journal of Oral Biosciences   52 ( Suppl )   121 - 121   2010.9

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    Language:Japanese   Publisher:(一社)歯科基礎医学会  

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  • ヘパラン硫酸の糖残基修飾は象牙質形成に関与する

    早野 暁, 黒坂 寛, 齋藤 正寛, 山城 隆

    Journal of Oral Biosciences   52 ( Suppl )   119 - 119   2010.9

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  • 歯の発生に対するWntシグナリングと糖鎖による修飾

    早野 暁, 黒坂 寛, 富田 奈緒, 柳田 剛志, 川邊 紀章, Dierks Thomas, Kalus Ina, 山城 隆

    組織培養研究   29 ( 1 )   112 - 112   2010.3

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  • Stage-specific embryonic antigen-4を用いたヒト歯根膜幹細胞の同定

    川邉 紀章, 村田 智子, 村上 薫, 石原 嘉人, 早野 暁, 黒坂 寛, 上岡 寛, 山本 照子, 山城 隆

    組織培養研究   29 ( 1 )   111 - 111   2010.3

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    Language:Japanese   Publisher:日本組織培養学会  

    DOI: 10.11418/jtca.29.111

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  • SSEA-4を用いたヒト歯根膜幹細胞の同定

    川邉 紀章, 村田 智子, 村上 薫, 早野 暁, 黒坂 寛, 上岡 寛, 山本 照子, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集   68回   180 - 180   2009.11

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  • 歯の発生におけるヘパラン硫酸の役割について

    黒坂 寛, 早野 暁, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集   67回   159 - 159   2008.9

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  • Coconspirator Statements and Coconspirator Confessions

    Hayano S.

    The Chuo law review   113 ( 1 )   439 - 474   2006.7

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    Language:Japanese   Publisher:Chuo University  

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  • Hearsay Rule and Finding of Fact

    Hayano S

    The Chuo law review   111 ( 5 )   291 - 314   2005.1

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Presentations

  • DENTAL AND CRANIOFACIAL CHARACTERISTICS OF A PATIENT WITH SINGLETON-MERTEN SYNDROME International conference

    The 9th international orthodontic congress 

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    Event date: 2020.10.4 - 2020.10.7

    Language:English   Presentation type:Poster presentation  

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  • Dental and Craniofacial Characteristics of a Patient with Regional Odontodysplasia

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    Event date: 2020.7.4 - 2020.7.5

    Language:Japanese   Presentation type:Poster presentation  

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  • Investigation of the molecular causes underlying physical abnormalities in Diamond-Blackfan anemia patients with RPL5 haploinsufficiency

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    Event date: 2019.11.20 - 2019.11.22

    Language:Japanese   Presentation type:Poster presentation  

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  • Increased BMP Signaling in Neural Crest Cells Causes Midfacial Clefting International conference

    Shawn A. Hallett, B.S., Jingwen Yang, Ph.D., Xia Liu, Ph.D., Satoru Hayano, Ph.D., Haichun Pan, M.S., Yuji Mishina, Ph.D.

    IADR/AADR/CADR General Session 2019  International Association for Dental Research

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    Event date: 2019.6.19 - 2019.6.22

    Language:English   Presentation type:Poster presentation  

    Venue:Vancouver, BC, Canada  

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  • Relation between chondrodysplasia and RPL5-associated cell death in patients with Diamond-Blackfan Anemia International conference

    95th European Orthodontic Society Congress 

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    Event date: 2019.6.17 - 2019.6.22

    Language:English   Presentation type:Poster presentation  

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  • ROLE OF THE INFERIOR ALVEOLAR NERVE IN THE MESENCHYMAL STEM/PROGENITOR CELLS International conference

    Satoru Hayano, Noriaki Kawanabe, Yuko Fukui, Hiroshi Kamioka

    94th European Orthodontic Society Congress  2018  European Orthodontic Society

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    Event date: 2018.6.17 - 2018.6.22

    Language:English   Presentation type:Poster presentation  

    Venue:Edinburgh, U.K.  

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  • Reduced mesenchymal stem cell and incisal malformation after inferior alveolar nerve neurectomy in rats

    2016 

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    Event date: 2016.11.7 - 2016.11.9

    Language:Japanese   Presentation type:Poster presentation  

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  • 神経提におけるBMPシグナリングの亢進が頭蓋顔面の発生に与える影響 International conference

    Satoru Hayano

    2013年度近畿大学生物理工学研究科 招待講演  2014  岸上 哲士

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    Event date: 2014.2.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:〒649-6493和歌山県紀の川市西三谷930 近畿大学大学院生物理工学研究科  

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  • 下歯槽神経切断によるラット下顎切歯の形態形成と歯原性間葉系幹細胞の減少

    第75回日本矯正歯科学会大会  2016 

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Awards

  • 94th EOS Congress Best poster award

    2018   European Orthodontic Society  

    Satoru Hayano

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Research Projects

  • I型インターフェロンが顎顔面の形態形成に及ぼす影響

    Grant number:19K10381  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    早野 暁, 川邉 紀章, 宝田 剛志

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    本研究の目的であるI型インターフェロンの機能亢進による全身性骨形成不全の発生機序を解明するため、Singleton-Merten Syndrome 患者および対照群となる健常患者の抜去歯から間葉系幹細胞を単離し、骨芽細胞への分化を試みた。また同時に、健常患者の抜去歯から単離した間葉系幹細胞にI型インターフェロンのリコンビナントプロテイン(IFN-alfa-2a, IFN-alfa-2b, IFN-beta)添加した実験群と、同じく健常患者由来の間葉系幹細胞にI型インターフェロンを添加しない対照群とで骨芽細胞への分化を試みた。どちらの実験でもI型インターフェロンが亢進している群において、重度の骨芽細胞への分化抑制が認められた。興味深いことに後者の実験から骨芽細胞への分化抑制は特にIFN-betaにおいて著しいことが分かった。
    これら一連の結果の原因を解明するため、培養後の細胞からRNAを単離し、qPCR法によって骨芽細胞分化マーカー、p53経路の活性、細胞死の状況を確認した。我々の当初の仮説では、I型インターフェロンの機能亢進が間葉系幹細胞においてp53細胞死経路を活性化することで骨芽細胞への分化抑制が生じていると考えていたが、予想に反してp53細胞死経路の活性化はI型インターフェロン亢進群では見られなかった。つまり、I型インターフェロンによる骨芽細胞への分化抑制はアポトーシスによるものではない可能性が示唆された。
    この結果はSingleton-Merten Syndrome の全身性骨形成不全の原因を解明する上で非常に重要な情報だと言える。

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  • Functional analysis of RPL5 using induced pluripotent stem cells from the patient with Diamond-Blackfan Anemia

    Grant number:17K17323  2017.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Hayano Satoru, SHIMADA Akira, SAITO Megumu, KAMIOKA Hiroshi, KAWANABE Noriaki, Fukui Yuko

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    Grant amount:\4030000 ( Direct expense: \3100000 、 Indirect expense:\930000 )

    In this study, we focused on Diamond-Blackfan anemia (DBA), which is characterized by red blood cell aplasia and craniofacial defects. iPS cells derived from DBA patient was used in this study. Our current study indicates that craniofacial defect is caused by activation of p53-mediated cell death pathway which is induced by interaction between ribosomal proteins and MDM2.

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  • Functional analysis of BMP signaling on tooth and limb development

    Grant number:15H06423  2015.08 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up  Grant-in-Aid for Research Activity Start-up

    Hayano Satoru

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    Grant amount:\2730000 ( Direct expense: \2100000 、 Indirect expense:\630000 )

    In this study, we investigated the BMP signaling function on tooth and limb formation during their development. We found that BMP ligands and phospho-SMADs were expressed in the area where programed cell death occurs. Further, tooth and limb bud organ culture with p53 inhibitor revealed that p53 pathway plays important role in tooth and limb formation.

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Class subject in charge

  • 人体発生学入門 (2021academic year) 第3学期  - 金曜日 2,3限

  • 学際的研究と臨床 (2021academic year) 第3学期

  • Clinical training:Early orthodontic treatment (2021academic year) special  - その他

  • Lecture and Research Projects:Early orthodontic treatment (2021academic year) special  - その他

  • Clinical training: Adult orthodontics (2021academic year) special  - その他

  • Lecture and Research Projects: Adult orthodontics (2021academic year) special  - その他

  • 歯の移動の臨床手技実習 (2021academic year) 1・2学期

  • Research Projects and Practicals: Orthodontics and Dentofacial Orthopedics I (2021academic year) special  - その他

  • Lecture and Research Projects: Orthodontics and Dentofacial Orthopedics I (2021academic year) special  - その他

  • Research Projects and Practicals: Orthodontics and Dentofacial Orthopedics II (2021academic year) special  - その他

  • Lecture and Research Projects: Orthodontics and Dentofacial Orthopedics II (2021academic year) special  - その他

  • Interdisciplinary Research and Clinic (2020academic year) Third semester  - 月1,月2

  • Clinical training:Early orthodontic treatment (2020academic year) special  - その他

  • Lecture and Research Projects:Early orthodontic treatment (2020academic year) special  - その他

  • Clinical training: Adult orthodontics (2020academic year) special  - その他

  • Lecture and Research Projects: Adult orthodontics (2020academic year) special  - その他

  • Research Projects and Practicals: Orthodontics and Dentofacial Orthopedics I (2020academic year) special  - その他

  • Lecture and Research Projects: Orthodontics and Dentofacial Orthopedics I (2020academic year) special  - その他

  • Research Projects and Practicals: Orthodontics and Dentofacial Orthopedics II (2020academic year) special  - その他

  • Lecture and Research Projects: Orthodontics and Dentofacial Orthopedics II (2020academic year) special  - その他

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