Updated on 2025/12/11

写真a

 
KOBUCHI Hirotsugu
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Lecturer
Position
Lecturer
External link

Degree

  • Ph. D. ( Okayama University )

Research Interests

  • 創薬化学

  • 腫瘍学

  • 生化学

  • 分子細胞生物学

  • Biochemistry

Research Areas

  • Life Science / Tumor biology

  • Life Science / Pharmaceutical chemistry and drug development sciences

  • Life Science / Medical biochemistry

  • Life Science / Molecular biology

  • Life Science / Physiology

Education

  • Okayama University   医学部  

    1991.4 - 1995.8

      More details

    Country: Japan

    researchmap

  • Okayama University    

    - 1995

      More details

  • Kochi Medical School   医学部  

    1988.4 - 1991.3

      More details

  • Kochi University   農学部   農芸化学科

    1984.4 - 1988.3

      More details

    Country: Japan

    researchmap

  • Kochi University    

    - 1988

      More details

Research History

  • 岡山大学学術研究院医歯薬学域, 臓器創造医療・生命医工学分野, 講師

    2024.9

      More details

  • Okayama University   細胞化学分野   Lecturer

    2021.4

      More details

    Country:Japan

    researchmap

  • Okayama University   Department of Cell Chemistry   Lecturer   博士

    2021.4

  • Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences   Department of Cell Chemistry   Assistant Professor

    2005.4

      More details

  • - Senior Assistant Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2005

      More details

  • Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences   Department of Cell Chemistry   Assistant Professor

    2001.4 - 2005.3

      More details

  • Senior Assistant Professor,Okayama University

    2001 - 2005

      More details

  • Okayama University   Medical School   Research Assistant

    1998.10 - 2001.3

      More details

  • Research Associate,Medical School,Okayama University

    1998 - 2001

      More details

  • University of California, Berkeley   Department of Molecular biology   Post doctoral fellow

    1994.12 - 1998.9

      More details

  • Researcher,University of California, Berkeley

    1994 - 1998

      More details

  • 倉敷成人病センター   研究員

    1988.4 - 1998.9

      More details

  • Researcher,Kurasiki Medical center

    1988 - 1998

      More details

▼display all

Professional Memberships

 

Papers

  • Protein N-myristoylation plays a critical role in the mitochondrial localization of human mitochondrial complex I accessory subunit NDUFB7 Reviewed

    Haruna Harada, Koko Moriya, Hirotsugu Kobuchi, Naotada Ishihara, Toshihiko Utsumi

    Scientific Reports   13 ( 1 )   22991 - 22991   2023.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Abstract

    The present study examined human N-myristoylated proteins that specifically localize to mitochondria among the 1,705 human genes listed in MitoProteome, a mitochondrial protein database. We herein employed a strategy utilizing cellular metabolic labeling with a bioorthogonal myristic acid analog in transfected COS-1 cells established in our previous studies. Four proteins, DMAC1, HCCS, NDUFB7, and PLGRKT, were identified as N-myristoylated proteins that specifically localize to mitochondria. Among these proteins, DMAC1 and NDUFB7 play critical roles in the assembly of complex I of the mitochondrial respiratory chain. DMAC1 functions as an assembly factor, and NDUFB7 is an accessory subunit of complex I. An analysis of the intracellular localization of non-myristoylatable G2A mutants revealed that protein N-myristoylation occurring on NDUFB7 was important for the mitochondrial localization of this protein. Furthermore, an analysis of the role of the CHCH domain in NDUFB7 using Cys to Ser mutants revealed that it was essential for the mitochondrial localization of NDUFB7. Therefore, the present results showed that NDUFB7, a vital component of human mitochondrial complex I, was N-myristoylated, and protein N-myrisotylation and the CHCH domain were both indispensable for the specific targeting and localization of NDUFB7 to mitochondria.

    DOI: 10.1038/s41598-023-50390-z

    researchmap

    Other Link: https://www.nature.com/articles/s41598-023-50390-z

  • A Novel 89Zr-labeled DDS Device Utilizing Human IgG Variant (scFv): “Lactosome” Nanoparticle-Based Theranostics for PET Imaging and Targeted Therapy Reviewed

    Melissa Siaw Han Lim, Takashi Ohtsuki, Fumiaki Takenaka, Kazuko Kobayashi, Masaru Akehi, Hirotaka Uji, Hirotsugu Kobuchi, Takanori Sasaki, Eiichi Ozeki, Eiji Matsuura

    Life   11 ( 2 )   158 - 186   2021.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    “Theranostics,” a new concept of medical advances featuring a fusion of therapeutic and diagnostic systems, provides promising prospects in personalized medicine, especially cancer. The theranostics system comprises a novel 89Zr-labeled drug delivery system (DDS), derived from the novel biodegradable polymeric micelle, “Lactosome” nanoparticles conjugated with specific shortened IgG variant, and aims to successfully deliver therapeutically effective molecules, such as the apoptosis-inducing small interfering RNA (siRNA) intracellularly while offering simultaneous tumor visualization via PET imaging. A 27 kDa-human single chain variable fragment (scFv) of IgG to establish clinically applicable PET imaging and theranostics in cancer medicine was fabricated to target mesothelin (MSLN), a 40 kDa-differentiation-related cell surface glycoprotein antigen, which is frequently and highly expressed by malignant tumors. This system coupled with the cell penetrating peptide (CPP)-modified and photosensitizer (e.g., 5, 10, 15, 20-tetrakis (4-aminophenyl) porphyrin (TPP))-loaded Lactosome particles for photochemical internalized (PCI) driven intracellular siRNA delivery and the combination of 5-aminolevulinic acid (ALA) photodynamic therapy (PDT) offers a promising nano-theranostic-based cancer therapy via its targeted apoptosis-inducing feature. This review focuses on the combined advances in nanotechnology and material sciences utilizing the “89Zr-labeled CPP and TPP-loaded Lactosome particles” and future directions based on important milestones and recent developments in this platform.

    DOI: 10.3390/life11020158

    researchmap

  • Lactosome-Conjugated siRNA Nanoparticles for Photo-Enhanced Gene Silencing in Cancer Cells. Reviewed International journal

    Melissa Siaw Han Lim, Yuki Nishiyama, Takashi Ohtsuki, Kazunori Watanabe, Hirotsugu Kobuchi, Kazuko Kobayashi, Eiji Matsuura

    Journal of pharmaceutical sciences   110 ( 4 )   1788 - 1798   2021.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The A3B-type Lactosome comprised of poly(sarcosine)3-block-poly(l-lactic acid), a biocompatible and biodegradable polymeric nanomicelle, was reported to accumulate in tumors in vivo via the enhanced permeability and retention (EPR) effect. Recently, the cellular uptake of Lactosome particles was enhanced through the incorporation of a cell-penetrating peptide (CPP), L7EB1. However, the ability of Lactosome as a drug delivery carrier has not been established. Herein, we have developed a method to conjugate the A3B-type Lactosome with ATP-binding cassette transporter G2 (ABCG2) siRNA for inducing in vitro apoptosis in the cancer cell lines PANC-1 and NCI-H226. The L7EB1 peptide facilitates the cellular uptake efficiency of Lactosome but does not deliver siRNA into cytosol. To establish the photoinduced cytosolic dispersion of siRNA, a photosensitizer loaded L7EB1-Lactosome was prepared, and the photosensitizer 5,10,15,20-tetra-kis(pentafluorophenyl)porphyrin (TPFPP) showed superiority in photoinduced cytosolic dispersion. We exploited the combined effects of enhanced cellular uptake by L7EB1 and photoinduced endosomal escape by TPFPP to efficiently deliver ABCG2 siRNA into the cytosol for gene silencing. Moreover, the silencing of ABCG2, a protoporphyrin IX (PpIX) transporter, also mediated photoinduced cell death via 5-aminolevulinic acid (ALA)-mediated PpIX accumulated photodynamic therapy (PDT). The synergistic capability of the L7EB1/TPFPP/siRNA-Lactosome complex enabled both gene silencing and PDT.

    DOI: 10.1016/j.xphs.2021.01.026

    PubMed

    researchmap

  • Identification and characterization of protein N-myristoylation occurring on four human mitochondrial proteins, SAMM50, TOMM40, MIC19, and MIC25. Reviewed

    Utsumi T, Matsuzaki K, Kiwado A, Tanikawa A, Kikkawa Y, Hosokawa T, Otsuka A, Iuchi Y, Kobuchi H, Moriya K

    PloS one   13 ( 11 )   e0206355   2018.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0206355

    PubMed

    researchmap

  • 89Zr標識ヒト抗体バリアントと新規DDSキャリアによるTheranostics技術 Reviewed

    竹中文章, 小林和子, 木村俊作, 小関英一, 大槻高史, 小渕浩嗣, 松浦栄次

    Drug Delivery System   33 ( 3 )   214 - 222   2018.7

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:日本DDS学会 ; 2000-  

    CiNii Article

    CiNii Books

    researchmap

    Other Link: https://search.jamas.or.jp/link/ui/2018300955

  • PET imaging utilizing 89Zr-labeled human antibody variant and theranostic technologies provided by a novel DDS carrier

    Fumiaki Takenaka, Kazuko Kobayashi, Shunsaku Kimura, Eiichi Ozeki, Takashi Ohtsuki, Hirotsugu Kobuchi, Eiji Matsuura

    Drug Delivery System   33 ( 3 )   214 - 222   2018

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japan Society of Drug Delivery System  

    DOI: 10.2745/dds.33.214

    Scopus

    researchmap

  • Phytoestrogen Suppresses Efflux of the Diagnostic Marker Protoporphyrin IX in Lung Carcinoma Reviewed

    Hirofumi Fujita, Keisuke Nagakawa, Hirotsugu Kobuchi, Tetsuya Ogino, Yoichi Kondo, Keiji Inoue, Taro Shuin, Toshihiko Utsumi, Kozo Utsumi, Junzo Sasaki, Hideyo Ohuchi

    CANCER RESEARCH   76 ( 7 )   1837 - 1846   2016.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1158/0008-5472.CAN-15-1484

    Web of Science

    researchmap

  • Identification of Human N-Myristoylated Proteins from Human Complementary DNA Resources by Cell-Free and Cellular Metabolic Labeling Analyses Reviewed

    Emi Takamitsu, Motoaki Otsuka, Tatsuki Haebara, Manami Yano, Kanako Matsuzaki, Hirotsugu Kobuchi, Koko Moriya, Toshihiko Utsumi

    PLOS ONE   10 ( 8 )   e0136360-e0136360   2015.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0136360

    Web of Science

    researchmap

  • Erythroid and megakaryocytic differentiation of K562 erythroleukemic cells by monochloramine Reviewed

    T. Ogino, H. Kobuchi, H. Fujita, A. Matsukawa, K. Utsumi

    FREE RADICAL RESEARCH   48 ( 3 )   292 - 302   2014.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3109/10715762.2013.865840

    Web of Science

    PubMed

    researchmap

  • Necrotic and apoptotic cells serve as nuclei for calcification on osteoblastic differentiation of human mesenchymal stem cells in vitro Reviewed

    Hirofumi Fujita, Masanao Yamamoto, Tetsuya Ogino, Hirotsugu Kobuchi, Naoko Ohmoto, Eriko Aoyama, Takashi Oka, Tohru Nakanishi, Keiji Inoue, Junzo Sasaki

    CELL BIOCHEMISTRY AND FUNCTION   32 ( 1 )   77 - 86   2014.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cbf.2974

    Web of Science

    researchmap

  • Acetyl-L-carnitine suppresses thyroid hormone-induced and spontaneous anuran tadpole tail shortening Reviewed

    Hideki Hanada, Hirotsugu Kobuchi, Masanao Yamamoto, Keiko Kashiwagi, Kenjiro Katsu, Toshihiko Utsumi, Akihiko Kashiwagi, Junzo Sasaki, Masayasu Inoue, Kozo Utsumi

    HEREDITAS   150 ( 1 )   1 - 9   2013.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1601-5223.2013.02284.x

    Web of Science

    PubMed

    researchmap

  • Mitochondrial Localization of ABC Transporter ABCG2 and Its Function in 5-Aminolevulinic Acid-Mediated Protoporphyrin IX Accumulation Reviewed

    Hirotsugu Kobuchi, Koko Moriya, Tetsuya Ogino, Hirofumi Fujita, Keiji Inoue, Taro Shuin, Tatsuji Yasuda, Kozo Utsumi, Toshihiko Utsumi

    PLOS ONE   7 ( 11 )   e50082-e50082   2012.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0050082

    Web of Science

    researchmap

  • Serum-dependent export of protoporphyrin IX by ATP-binding cassette transporter G2 in T24 cells Reviewed

    Tetsuya Ogino, Hirotsugu Kobuchi, Kazuaki Munetomo, Hirofumi Fujita, Masanao Yamamoto, Toshihiko Utsumi, Keiji Inoue, Taro Shuin, Junzo Sasaki, Masayasu Inoue, Kozo Utsumi

    MOLECULAR AND CELLULAR BIOCHEMISTRY   358 ( 1-2 )   297 - 307   2011.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s11010-011-0980-5

    Web of Science

    researchmap

  • Mechanism of A23187-induced apoptosis in HL-60 cells: Dependency on mitochondrial permeability transition but not on NADPH oxidase Reviewed

    Noriko Kajitani, Hirotsugu Kobuchi, Hirofumi Fujita, Hiromi Yano, Takuzo Fujiwara, Tatsuji Yasuda, Kozo Utsumi

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   71 ( 11 )   2701 - 2711   2007.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.70304

    Web of Science

    PubMed

    researchmap

  • Cell-permeable cAMP analog suppresses 6-hydroxydopamine-induced apoptosis in PC12 cells through the activation of the Akt pathway Reviewed

    Hirofumi Fujita, Tetsuya Ogino, Hirotsugu Kobuchi, Takuzo Fujiwara, Hiromi Yano, Jitsuo Akiyama, Kozo Utsumi, Junzo Sasaki

    BRAIN RESEARCH   1113 ( 1 )   10 - 23   2006.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.brainres.2006.06.079

    Web of Science

    researchmap

  • Induction of cell death in human papillomavirus 18-positive cervical cancer cells by E6 siRNA Reviewed

    K Yamato, J Fen, H Kobuchi, Y Nasu, T Yamada, T Nishihara, Y Ikeda, M Kizaki, M Yoshinouchi

    CANCER GENE THERAPY   13 ( 3 )   234 - 241   2006.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/sj.cgt.7700891

    Web of Science

    researchmap

  • アポトーシスとミトコンドリアー神経細胞を加味して Reviewed

    藤田洋史, 小渕浩嗣, 内海耕慥

    Clinical Neuroscience   24 ( 6 )   643 - 646   2006

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • 志賀毒素誘導の腎上皮様Vero細胞のアポトーシスにおけるAktの関与(Involvement of Akt in Shiga toxin-induced apoptosis of renal epithelial cells)

    Kobuchi Hirotsugu, Kaihara Keiko, Utsumi Kozo, Yasuda Tatsuji

    生化学   76 ( 8 )   1089 - 1089   2004.8

     More details

    Language:English   Publisher:(公社)日本生化学会  

    researchmap

  • Involvement of ceramide in the mechanism of Cr(VI)-induced apoptosis of CHO cells Reviewed

    S Muranaka, T Kanno, H Fujita, H Kobuchi, J Akiyama, T Yasuda, K Utsumi

    FREE RADICAL RESEARCH   38 ( 6 )   613 - 621   2004.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/10715760410001694035

    Web of Science

    researchmap

  • Cholesteryl-hemisuccinate-induced apoptosis of promyelocytic leukemia HL-60 cells through a cyclosporin A-insensitive mechanism Reviewed

    K Yamada, K Arita, H Kobuchi, S Yamamoto, T Yoshioka, H Tamai, K Utsumi

    BIOCHEMICAL PHARMACOLOGY   65 ( 3 )   339 - 348   2003.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0006-2952(02)01518-6

    Web of Science

    researchmap

  • Mitochondria and apoptosis. Reviewed

    Kanno T, Utsumi K, Kobuchi H

    Nihon rinsho. Japanese journal of clinical medicine   60 Suppl 4   189 - 193   2002.4

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    PubMed

    researchmap

  • Mevastatin, an inhibitor of HMG-CoA reductase, induces apoptosis, differentiation and Rap1 expression in HL-60 cells Reviewed

    Tomoko Kanno, Hirotsugu Kobuchi, Noriko Kajitani, Toshihiko Utsumi, Hiromi Yano, Alan A. Horton, Tatsuji Yasuda, Kozo Utsumi

    Physiological Chemistry and Physics and Medical NMR   34 ( 1 )   1 - 15   2002

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Scopus

    PubMed

    researchmap

  • ミトコンドリアと細胞死 Reviewed

    菅野智子, 小渕浩嗣, 内海耕慥

    日本臨床   60 ( 4 )   189 - 193   2002

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • Mechanism of apoptosis in HL-60 cells induced by n-3 and n-6 polyunsaturated fatty acids Reviewed

    K Arita, H Kobuchi, T Utsumi, Y Takehara, J Akiyama, AA Horton, K Utsumi

    BIOCHEMICAL PHARMACOLOGY   62 ( 7 )   821 - 828   2001.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0006-2952(01)00723-7

    Web of Science

    researchmap

  • 志賀毒素による細胞死誘導とその機序 Reviewed

    小渕 浩嗣

    岡山大学アイソトープセンターニュース   11   4 - 7   2001

     More details

    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)  

    researchmap

  • Mechanism of dibucaine-induced apoptosis in promyelocytic leukemia cells (HL-60) Reviewed

    K Arita, T Utsumi, A Kato, T Kanno, H Kobuchi, B Inoue, J Akiyama, K Utsumi

    BIOCHEMICAL PHARMACOLOGY   60 ( 7 )   905 - 915   2000.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0006-2952(00)00406-8

    Web of Science

    researchmap

  • Mechanism of alpha-tocopheryl succinate-induced apoptosis of promyelocytic leukemia cells Reviewed

    S Yamamoto, H Tamai, R Ishisaka, T Kanno, K Arita, H Kobuchi, K Utsumi

    FREE RADICAL RESEARCH   33 ( 4 )   407 - +   2000

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    researchmap

  • Quercetin inhibits inducible ICAM-1 expression in human endothelial cells through the JNK pathway Reviewed

    H Kobuchi, S Roy, CK Sen, HG Nguyen, L Packer

    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY   277 ( 3 )   C403 - C411   1999.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    researchmap

  • Assay of inducible form of nitric oxide synthase activity: Effect of flavonoids and plant extracts Reviewed

    H Kobuchi, F Virgili, L Packer

    NITRIC OXIDE, PT C   301   504 - 513   1999

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0076-6879(99)01113-1

    Web of Science

    researchmap

  • Antioxidant regulation of phorbol ester-induced adhesion of human jurkat T-cells to endothelial cells Reviewed

    S Roy, CK Sen, H Kobuchi, L Packer

    FREE RADICAL BIOLOGY AND MEDICINE   25 ( 2 )   229 - 241   1998.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0891-5849(98)00062-8

    Web of Science

    researchmap

  • Antioxidant regulation of phorbol ester-induced adhesion of human jurkat T-cells to endothelial cells Reviewed

    S Roy, CK Sen, H Kobuchi, L Packer

    FREE RADICAL BIOLOGY AND MEDICINE   25 ( 2 )   229 - 241   1998.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0891-5849(98)00062-8

    Web of Science

    PubMed

    researchmap

  • Procyanidins extracted from pinus Maritima (Pycnogenol((R))): Scavengers of free radical species and modulators of nitrogen monoxide metabolism in activated murine raw 264.7 macrophages Reviewed

    F Virgili, H Kobuchi, L Packer

    FREE RADICAL BIOLOGY AND MEDICINE   24 ( 7-8 )   1120 - 1129   1998.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0891-5849(97)00430-9

    Web of Science

    PubMed

    researchmap

  • Ginkgo biloba extract (EGb 761): antioxidant properties and regulation of gene expression Reviewed

    S Roy, H Kobuchi, CK Sen, K Gohil, MT Droy-Lefaix, L Packer

    GINKGO BILOBA EXTRACT (EGB 761): LESSONS FROM CELL BIOLOGY   7   1 - 17   1998

     More details

    Language:English   Publishing type:Research paper (international conference proceedings)  

    Web of Science

    researchmap

  • Monochloramine inhibits phorbol ester-inducible neutrophil respiratory burst activation and T cell interleukin-2 receptor expression by inhibiting inducible protein kinase C activity Reviewed

    T Ogino, H Kobuchi, CK Sen, S Roy, L Packer, JJ Maguire

    JOURNAL OF BIOLOGICAL CHEMISTRY   272 ( 42 )   26247 - 26252   1997.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.272.42.26247

    Web of Science

    researchmap

  • Bio-Normalizer modulates interferon-gamma-induced nitric oxide production in the mouse macrophage cell line RAW 264.7 Reviewed

    H Kobuchi, L Packer

    BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL   43 ( 1 )   141 - 152   1997.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    researchmap

  • Ginkgo biloba extract (EGb 761): Inhibitory effect on nitric oxide production in the macrophage cell line RAW 264.7 Reviewed

    H Kobuchi, MT DroyLefaix, Y Christen, L Packer

    BIOCHEMICAL PHARMACOLOGY   53 ( 6 )   897 - 903   1997.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0006-2952(96)00873-8

    Web of Science

    researchmap

  • Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilization Reviewed

    D Han, G Handelman, L Marcocci, CK Sen, S Roy, H Kobuchi, HJ Tritschler, L Flohe, L Packer

    BIOFACTORS   6 ( 3 )   321 - 338   1997

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    researchmap

  • Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilization Reviewed

    D Han, G Handelman, L Marcocci, CK Sen, S Roy, H Kobuchi, HJ Tritschler, L Flohe, L Packer

    BIOFACTORS   6 ( 3 )   321 - 338   1997

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    PubMed

    researchmap

  • Efficacy of hypochlorous acid scavengers in the prevention of protein carbonyl formation Reviewed

    LJ Yan, MG Traber, H Kobuchi, S Matsugo, HJ Tritschler, L Packer

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   327 ( 2 )   330 - 334   1996.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1006/abbi.1996.0130

    Web of Science

    researchmap

  • INHIBITION OF NEUTROPHIL SUPEROXIDE GENERATION BY HYPERICIN, AN ANTIRETROVIRAL AGENT Reviewed

    T NISHIUCHI, T UTSUMI, T KANNO, Y TAKEHARA, H KOBUCHI, T YOSHIOKA, AA HORTON, T YASUDA, K UTSUMI

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   323 ( 2 )   335 - 342   1995.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1006/abbi.1995.9956

    Web of Science

    PubMed

    researchmap

  • Bio-Catalyzer alpha center dot rho No 11 (Bio-Normalizer) supplementation: Effect on oxidative stress to isolated rat hearts. Reviewed

    N Haramaki, L Marcocci, R DAnna, LJ Yan, H Kobuchi, L Packer

    BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL   36 ( 6 )   1263 - 1268   1995.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    PubMed

    researchmap

  • PHOTOACTIVATED INHIBITION OF SUPEROXIDE GENERATION AND PROTEIN-KINASE-C ACTIVITY IN NEUTROPHILS BY BLEPHARISMIN, A PROTOZOAN PHOTODYNAMICALLY ACTIVE PIGMENT Reviewed

    Y WATANABE, K EDASHIGE, H KOBUCHI, Y KATO, T MATSUOKA, T UTSUMI, T YOSHIOKA, AA HORTON, K UTSUMI

    BIOCHEMICAL PHARMACOLOGY   49 ( 4 )   529 - 536   1995.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/0006-2952(94)00409-F

    Web of Science

    PubMed

    researchmap

  • LIGHT-DEPENDENT INHIBITION OF PROTEIN-KINASE-C AND SUPEROXIDE GENERATION OF NEUTROPHILS BY HYPERICIN, AN ANTIRETROVIRAL AGENT Reviewed

    T UTSUMI, M OKUMA, T UTSUMI, T KANNO, T YASUDA, H KOBUCHI, AA HORTON, K UTSUMI

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   316 ( 1 )   493 - 497   1995.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1006/abbi.1995.1065

    Web of Science

    PubMed

    researchmap

  • AAPHによるリポソームの区画性低下に及ぼすトコフェロールの影響 Reviewed

    竹原良記, 小渕浩嗣, 菅野智子, 保田立二, 秋山実男, 吉岡 保, 内海耕慥

    ビタミンE研究の進歩   5   87 - 92   1995

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • SELECTIVE-INHIBITION OF HUMAN TNF-ALPHA ACTION BY FLECAINIDE ACETATE, AN ANTIARRHYTHMIC DRUG Reviewed

    S NOJIMA, H KOBUCHI, T SAIBARA, T YASUDA, K UTSUMI

    PHYSIOLOGICAL CHEMISTRY AND PHYSICS AND MEDICAL NMR   27 ( 2 )   77 - 89   1995

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    PubMed

    researchmap

  • INHIBITION OF STIMULUS-SPECIFIC NEUTROPHIL SUPEROXIDE GENERATION BY ALPHA-TOCOPHEROL Reviewed

    T KANNO, T UTSUMI, H KOBUCHI, Y TAKEHARA, J AKIYAMA, T YOSHIOKA, AA HORTON, K UTSUMI

    FREE RADICAL RESEARCH   22 ( 5 )   431 - 440   1995

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    PubMed

    researchmap

  • キサンチン酸化におけるルミノール化学発光の解析 Reviewed

    小渕浩嗣, 秋丸国広, 岡添陽子, 佐藤英介, 枝重圭祐, 吉岡保, 内海耕慥

    過酸化脂質研究   17 ( 2 )   132 - 135   1993

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • EFFECT OF INDOMETHACIN AND ASPIRIN ON THE TNF-ALPHA-INDUCED PRIMING AND PROTEIN TYROSYL PHOSPHORYLATION OF HUMAN NEUTROPHILS Reviewed

    K MINAKAMI, Y WATANABE, M MIYAHARA, H KOBUCHI, T KURASHIGE, K UTSUMI

    PHYSIOLOGICAL CHEMISTRY AND PHYSICS AND MEDICAL NMR   25 ( 1 )   55 - 67   1993

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    PubMed

    researchmap

  • 好中球の活性化とカルシウム動態 Reviewed

    小渕浩嗣, 吉岡 保, 内海耕慥

    Clinical Calcium   3 ( 12 )   31 - 38   1993

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • INHIBITION OF NEUTROPHIL PRIMING AND TYROSYL PHOSPHORYLATION BY CEPHARANTHINE, A NONSTEROIDAL ANTIINFLAMMATORY DRUG Reviewed

    H KOBUCHI, MJ LI, T MATSUNO, T YASUDA, K UTSUMI

    CELL STRUCTURE AND FUNCTION   17 ( 6 )   385 - 393   1992.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1247/csf.17.385

    Web of Science

    researchmap

  • NEUTROPHIL PRIMING BY GRANULOCYTE COLONY STIMULATING FACTOR AND ITS MODULATION BY PROTEIN-KINASE INHIBITORS Reviewed

    M TANIMURA, H KOBUCHI, T UTSUMI, T YOSHIOKA, S KATAOKA, Y FUJITA, K UTSUMI

    BIOCHEMICAL PHARMACOLOGY   44 ( 6 )   1045 - 1052   1992.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/0006-2952(92)90366-Q

    Web of Science

    PubMed

    researchmap

  • NEUTROPHIL PRIMING BY GRANULOCYTE COLONY STIMULATING FACTOR AND ITS MODULATION BY PROTEIN-KINASE INHIBITORS Reviewed

    M TANIMURA, H KOBUCHI, T UTSUMI, T YOSHIOKA, S KATAOKA, Y FUJITA, K UTSUMI

    BIOCHEMICAL PHARMACOLOGY   44 ( 6 )   1045 - 1052   1992.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/0006-2952(92)90366-Q

    Web of Science

    researchmap

  • DYNAMIC ASPECTS OF OVARIAN SUPEROXIDE-DISMUTASE ISOZYMES DURING THE OVULATORY PROCESS IN THE RAT Reviewed

    EF SATO, H KOBUCHI, K EDASHIGE, M TAKAHASHI, T YOSHIOKA, K UTSUMI, M INOUE

    FEBS LETTERS   303 ( 2-3 )   121 - 125   1992.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    PubMed

    researchmap

  • Neutrophil Priming by Cytokines : in Relation to the Protein Tyrosyl Phosphorylation of Neutrophils Proteins. Reviewed

    WATANABE Yoshiya, KOBUCHI Hirotsugu, AKIMARU Kunihiro, SATO Eisuke F., MINAKAMI Kayoko, KANBARA Tetsuya, TOMODA Miho, KOBAYASHI Sumio, YOSHIOKA Tamotsu, UTSUMI Kozo

    Research reports of Kochi Medical School Liberal arts   8   45 - 56   1992

     More details

    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)   Publisher:Kochi University  

    We have investigated the effect of tumor necrosis factor-α (TNF-α) and granulocyte colony stimulating factor (G-CSF) on human peripheral neutrophils (HPPMN). TNF-α and G -CSF enhanced fonylmethionyl-1eucyl-phenylalanine (FMLP) -stimiulated O^∸_2 generation of HPPMN in concentration and time dependent manners. The enhancement of 0^∸_2 generation was inhibited by tyrosine kinase inhibitors. These cytokins also enhanced the tyrosyl phosphorylation of neutrophil proteins, such as 115,110,98,83,72,70,60,54 kDa and many of low molecular weight proteins. Both tyrosine kinase and phosphotyr-osine phosphatase might regulate the protein tyrosyl phosphorylation since the phosphorylation inhibited by tyrosine kinase inhibitor, genistein. The TNF-α enhanced tyrosyl phosphorylation of 115 and 108 kDa proteins, which distributed in the membrane fraction, was increased in manners of incubation and concentration dependent. The kinetics of TNF-α enhanced FMLP -dependent O^∸_2 generation was quite similar to that of tyrosyl phosphorylation of neutrophil proteins by TNF-α Furthermore, cepharanthine specifically inhibited either TNF-α or G-CSF enhanced FMLP-stimulated 0^∸_2 generation and tyrosyl phophorylation of some neutrophil proteins, 115 kDa. Taken together with this finding, it is concluded that substrate proteins for tyrosin kinases might act as critical effector molecules in the mechanism for priming in neutrophils.

    CiNii Article

    CiNii Books

    researchmap

  • Mechanism of Superoxide Generation in Human Peripheral Neutrophils : Its Priming, Stimulation, and Protein Kinase Specificities Reviewed

    AKIMARU Kunihiro, AKIMARU Yoko, SATO Eisuke F., EDASHIGE Keisuke, TAKAHASHI Masahiko, TANIMURA Masanobu, KOBUCHI Hirotsugu, KOBAYASHI Sumio, YOSHIOKA Tamotsu, UTSUMI Kozo

    Research reports of Kochi Medical School Liberal arts   7   47 - 59   1991

     More details

    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)   Publisher:Kochi University  

    Healthy human peripheral neutrophils (HPPMN) are not primed, and have weak responses to stimuli which activate HPPMN through their membrane receptors. Recombinant human tumor necrosis factor-α (rHuTNF) and recombinant granulocyte colony stimulation factor (rG-CSF) primed HPPMN. Superoxide (O^&minusd;_2) generation by formylmethionyl-leucylphenylalanine (FMLP) or opsonized zymosan (OZ) was enhanced by these primers. However, O^&minusd;_2 generation induced by phorbol myristate acetate (PMA) or dioctanoylglycerol (DOG) was not enhanced by the primers. Receptor mediated O^&minusd;_2 generation in rHuTNF primed neutrophils was inhibited by the genistein or alpha-cyan0-3-ethoxy-4-hydroxy-5phenylthiomethylcinnamamide (ST 638), inhibitors of tyrosine kinase (TK). But it was enhanced by 1-(5-isoquinoline sulfonyl)-3-methyl-piperazine (H-7) or staurosporine, inhibitors of Ca^<++>_ and phospholipid-dependent protein kinase (PKO, in their concentration dependent manner. On the contrary, O^&minusd;_2 generation of HPPMN at higher concentration of PMA or DOG was rather stimulated by genistein or ST 638 and was inhibited by H-7 or staurosporine. These results suggest that protein kinases participates in the NADPH-oxidase activation of neutrophils and that two pathways exist in the NADPH-oxidase activation : one in PMA- or DOG-stimulated PKC-dependent pathway and the other in FMLP-stimulated TK-dependent pathway. Moreover, it suggests that TK might be involved in the reaction of neutrophil priming with various ligands.

    CiNii Article

    CiNii Books

    researchmap

  • 口腔内多形核白血球の刺激応答性 Reviewed

    佐藤英介, 秋丸国広, 枝重圭祐, 片岡真一, 山本昌澄, 森下誠治, 高橋正彦, 小渕浩嗣, 内海耕慥

    医学のあゆみ   158 ( 11 )   761 - 762   1991

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    researchmap

  • INHIBITION OF ACTIVE OXYGEN GENERATION IN GUINEA-PIG NEUTROPHILS BY BISCOCLAURINE ALKALOIDS Reviewed

    T MATSUNO, K ORITA, K EDASHIGE, H KOBUCHI, EF SATO, B INOUYE, M INOUE, K UTSUMI

    BIOCHEMICAL PHARMACOLOGY   39 ( 7 )   1255 - 1259   1990.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/0006-2952(90)90271-L

    Web of Science

    PubMed

    researchmap

  • Levels of sister-chromatid exchanges in hybrids between Bloom syndrome B-lymphoblastoid cells and various cell lines with lymphoid malignancy. Reviewed

    Shiraishi Y, Kobuchi H, Utsumi K, Minowada J

    Mutat. Res.   243 ( 1 )   13 - 20   1990.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PubMed

    researchmap

  • LEVELS OF SISTER-CHROMATID EXCHANGES IN HYBRIDS BETWEEN BLOOM SYNDROME B-LYMPHOBLASTOID CELLS AND VARIOUS CELL-LINES WITH LYMPHOID MALIGNANCY Reviewed

    Y SHIRAISHI, H KOBUCHI, K UTSUMI, J MINOWADA

    MUTATION RESEARCH   243 ( 1 )   13 - 20   1990.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    researchmap

  • Levels of sister-chromatid exchanges in hybrids between Bloom syndrome B-lymphoblastoid cells and various cell lines with lymphoid malignancy Reviewed

    Yukimasa Shiraishi, Hirotsugu Kobuchi, Kozo Utsumi, Jun Minowada

    Mutation Research Letters   243 ( 1 )   13 - 20   1990

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/0165-7992(90)90117-3

    Scopus

    PubMed

    researchmap

  • EXPRESSION OF THE CDNA-ENCODING LIPOCORTIN-LIKE 39-KDA PROTEIN OF GUINEA-PIG NEUTROPHILS IN YEAST - PURIFICATION AND BIOLOGICAL CHARACTERIZATION Reviewed

    EF SATO, Y TANAKA, K EDASHIGE, H KOBUCHI, S MORISHITA, YM SUGINO, M INOUE, K UTSUMI

    FEBS LETTERS   255 ( 2 )   231 - 236   1989.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/0014-5793(89)81097-X

    Web of Science

    PubMed

    researchmap

  • Distribution of EGF Receptor/Kinase Substrates in Various Organs of Guinea Pig Reviewed

    UTSUMI Kozo, MORISHITA Seiji, SATO Eisuke F., SUGINO Yasuo M., KOBUCHI Hirotsugu

    Research reports of Kochi Medical School Liberal arts   5   39 - 48   1989

     More details

    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)   Publisher:Kochi University  

    Distribution of EGF receptor/kinase substrates in cytoplasmic proteins of guinea pig various organs was studied. 35kDa and 38kDa proteins in SDS-PAGE were detected as substrates for the enzyme in lung, spleen, stomach, urinary bladder and skin. 35kDa protein is confermed as lipocortin-like 39K protein of neutrophils by the method of immunoblotting using antibody of 39K protein. The Ca^<2+>-dependent membrane binding activity protein was an important character for the protein phosphorylation by this enzyme. A recombinant 39K protein was also phosphorylated by EGF receptor/kinase and the amount of phosphorylated 38kDa detected on SDS-PAGE was increased by the time of incubation.

    CiNii Article

    CiNii Books

    researchmap

▼display all

Books

  • 両備てい園記念財団 平成18年度 研究報告書

    2008 

     More details

  • 生物学に関する試験研究論叢 23

    2008 

     More details

  • 浜崎癌研究助成 平成18年度 研究報告書

    2007 

     More details

  • 財団法人ウエスコ学術振興財団 平成17年度(2005〜2006)研究助成報告

    2006 

     More details

  • 財団法人ウエスコ学術振興財団 平成16年度(2004〜2005)研究助成報告

    2005 

     More details

  • 財団法人ウエスコ学術振興財団 平成15年度研究助成報告

    2004 

     More details

  • 財団法人両備てい園記念財団研究助成報告 生物学に関する試験研究論叢

    2004 

     More details

  • 新ミトコンドリア学

    小渕浩嗣( Role: Sole author ,  細胞内および単離ミトコンドリアの膜電位測定)

    共立出版  2001 

     More details

  • Antioxidant Food Supplements in Human Health (L. Packer, and T. Yoshikawa, eds.)

    Virgili F, Kobuchi H, Noda Y, Cossins E, Packer L( Role: Joint author ,  Procyanidins from Pinus marittima Bark: Antioxidant Activity, Effects on the Immune System, and Modulation of Nitrogen Monoxide Metabolism.)

    Academic Press  1999 

     More details

  • Antioxidant food supplements in human health

    Academic Press  1999 

     More details

  • Methods Enzymol.

    Kobuchi H, Virgili F, Packer L( Role: Joint author ,  Assay of inducible form of nitric oxide synthase activity: effect of flavonoids and plant extracts.)

    Elsevier  1999 

     More details

  • Antioxidant Food Supplements in Human Health (L. Packer, M. Hiramatsu, and T. Yoshikawa, eds.)

    Kobuchi H, Haramaki N, Marcocci L, Packer L( Role: Joint author ,  Biological Effects of the Fermentation Product of Carica papaya (Bio-Normalizer).)

    Academic Press  1999 

     More details

  • Flavonoids in Health and Disease (C. A. Rice-Evans, and L. Packer, eds.)

    Virgili F, Kobuchi H, Packer L( Role: Joint author ,  Antioxidant properties and modulation of inducible NO synthase activity in activated macrophages by procyanidins extracted from Pinus maritima (Pycnogenol®).)

    Marcel Dekker, Inc.  1998 

     More details

  • Advances in Ginkgo biloba Extract Research, vol. 7 Ginkgo biloba Extract (EGb 761): Lessons from Cell Biology (L. Packer, and Y. Christen, eds.)

    Roy S, Kobuchi H, Sen C.K, Gohil K, Droy-Lefaix M.T, Packer L( Role: Joint author ,  Ginkgo biloba extract (EGb 761): antioxidant properties and regulation of gene expression.)

    Elsevier  1998 

     More details

  • Molecular mechanisms and health effects (Packer, L. Traber, M. G., and Xin, W. eds.)

    Packer L, Marcocci L, Haramaki N, Kobuchi H, Christen Y, Droy-Lefaix M.T( Role: Joint author ,  Antioxidant properties of Ginkgo biloba extract EGb 761 and clinical implications.)

    AOCS Press  1996 

     More details

▼display all

MISC

  • ビタミンE及びコレステロールのコハク酸エステルによるヒト前骨髄性白血病細胞(HL-60)のアポトーシス誘導とその比較

    山田 勝彦, 山本 真司, 竹原 良記, 小渕 浩嗣, 吉岡 保, 内海 耕慥, 玉井 浩

    ビタミン   76 ( 3 )   158 - 158   2002

     More details

    Language:Japanese   Publisher:公益社団法人 日本ビタミン学会  

    CiNii Article

    CiNii Books

    researchmap

  • Antioxidants regulate agonists-induced cell adhesion molecule expression and function

    S Roy, CK Sen, H Kobuchi, L Packer

    FASEB JOURNAL   13 ( 7 )   A1581 - A1581   1999.4

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • French Maritime Pine bark extract: Antioxidant activity and effects on regulation of nitric oxide metabolism and cell adhesion

    L Packer, T Bito, E Cossins, H Kobuchi, H Moini, Y Noda, G Rimbach, S Roy, J Vaya, F Virgili

    FASEB JOURNAL   13 ( 4 )   A546 - A546   1999.3

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Effect of polyphenols extracted from pine bark (pycnogenol) on nitric oxide metabolism and their role in the antioxidant network: Development of tools for the management of free-radical associated diseases?

    L Packer, F Virgill, H Kobuchi, R Lee, E Cossins

    FASEB JOURNAL   12 ( 5 )   A814 - A814   1998.3

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Regulation of phorbol ester induced adhesion of human jurkat T-cells to endothelial cells by the thiol antioxidant alpha-lipoate

    S Roy, CK Sen, H Kobuchi, L Packer

    FASEB JOURNAL   12 ( 5 )   A772 - A772   1998.3

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Antioxidant regulation of ICAM-1 expression

    CK Sen, S Roy, H Kobuchi, L Packer

    FREE RADICAL BIOLOGY AND MEDICINE   25   S52 - S52   1998

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • Pycnogenol (R): Antioxidant mechanism and regulation of no metabolism and cell adhesion

    L Packer, Y Cossins, Y Noda, F Virgili, H Kobuchi, S Roy, T Bito, J Vaya, H Moini

    FREE RADICAL BIOLOGY AND MEDICINE   25   S36 - S36   1998

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • 7.胎盤組織における過酸化脂質とビタミンE : ビタミンE研究会第2回研究発表要旨

    吉岡 保, 竹原 良記, 藤井 好孝, 小渕 浩嗣

    ビタミン   64 ( 11 )   671 - 672   1990.11

     More details

    Language:Japanese   Publisher:日本ビタミン学会  

    CiNii Article

    CiNii Books

    researchmap

  • 17.ヒト卵胞液中の過酸化脂質濃度とビタミンE及びステロイドホルモンの関連性(第1回研究発表要旨,ビタミンE研究会)

    竹原 良記, 藤井 好孝, 小渕 浩嗣, 橋本 光月, 本山 洋明, 吉岡 保

    ビタミン   63 ( 11 )   587 - 588   1989.11

     More details

    Language:Japanese   Publisher:日本ビタミン学会  

    CiNii Article

    CiNii Books

    researchmap

▼display all

Presentations

  • 効率的なsiRNAの光依存的細胞内送達能を持つ生分解性ナノキャリアの開発

    田中七星, 渡邉和則, 小渕浩嗣, 久保貴紀, 松浦栄次, 大槻高史

    第44回日本バイオマテリアル学会  2022.11.21 

     More details

    Event date: 2022.11.21 - 2022.11.22

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • N-myristoylation dependent mitochondrial targeting of NDUFB7, an accessory subunit of mitochondrial respiratory complex I

    2021.11.3 

     More details

    Event date: 2021.11.3 - 2021.11.5

    Language:Japanese  

    researchmap

  • N-ミリストイル化依存的にミトコンドリアに特異的に局在するタンパク質NDUFB7の発見

    原田春菜, 岩本奈津子, 西上美里, 七田真由, 守屋康子, 小渕浩嗣, 内海俊彦

    第62回 日本生化学会 中国・四国支部例会  2021.9.11 

     More details

    Event date: 2021.9.10 - 2021.9.11

    Language:Japanese  

    researchmap

  • Identification of N-myristoylated protein specifically localized to mitochondria by protein N-myristoylation

    2021.3.18 

     More details

    Event date: 2021.3.18 - 2021.3.21

    researchmap

  • N-ミリストイル化されたミトコンドリアタンパク質の探索

    原田春菜, 岩本奈津子, 西谷美里, 守屋康子, 小渕浩嗣, 内海俊彦

    第61回 日本生化学大会中四国支部例会  2020.7.18 

     More details

    Event date: 2020.7.18 - 2020.7.20

    Language:Japanese  

    researchmap

  • siRNAを用いたABCG2発現抑制と細胞死誘導の解析

    小渕浩嗣, 藤田洋史

    第61回 日本生化学大会中四国支部例会  2020.7.18 

     More details

    Event date: 2020.7.18 - 2020.7.20

    Language:Japanese  

    researchmap

  • ABCG2トランスポーターの発現抑制によるアポトーシス誘導

    小渕浩嗣, 藤田洋史

    第92回 日本生化学大会  2019.9.18 

     More details

    Event date: 2019.9.18 - 2019.9.20

    Language:English  

    researchmap

  • siRNAを腫瘍組織へ送達するための細胞内侵入性ミセル型キャリアの開発

    西山雄基, 小渕浩嗣, 松浦栄次, 小関英一, 渡邉和則, 大槻高史

    日本核酸医薬学会第5回年会  2019.7.10 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ABCG2 siRNA導入によるがん細胞のアポトーシス誘導

    小渕浩嗣, 藤田洋史

    第90回日本生化学会大会  2017.12.6 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • Small interfering RNA targeting of the ABCG2 gene induces apoptosis of cancer cells.

    Small interfering RNA targeting of the ABCG2 gene induces apoptosis of cancer cells.  2017 

     More details

  • ヘム代謝関連遺伝子のRNAi効果による光線力学療法の改善

    小渕浩嗣, 藤田洋史

    第89回日本生化学会大会  2016.9.25 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • ヘム代謝制御に基づく光感受性物質protoporphyrin IX蓄積機構の研究

    藤田洋史, 永川恵介, 小渕浩嗣, 荻野哲也, 近藤洋一, 井上啓史, 執印太郎, 内海俊彦, 内海耕慥, 佐々木順造, 大内淑代

    第69回 日本酸化ストレス学会学術集会  2016.8.30 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • N-ミリストイル化されたミトコンドリアタンパク質SAMM50, TOMM40, CHCHD3, CHCHD6の間に生ずる相互作用の解析

    松崎嘉奈子, 岩永友花, 守屋康子, 小渕浩嗣, 内海俊彦

    第57回 日本生化学会 中国・四国支部例会  2016.5.27 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • がんの光線力学的治療の効率化におけるABCG2抑制の有用性

    小渕浩嗣, 藤田洋史

    第68回 日本酸化ストレス学会学術集会  2015.6.11 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • アミノレブリン酸を用いた光線力学的作用におけるABCトランスポーターG2阻害の効果

    小渕浩嗣, 藤田洋史

    第4回 ポルフィリンーALA学会年会  2014.4.26 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • アセチル-L-カルニチンによる無尾両生類幼生尾部短縮の抑制

    花田秀樹, 小渕浩嗣, 柏木啓子, 内海俊彦, 井上正康, 佐々木順造, 勝賢二郎, 佐藤英介, 内海耕慥, 柏木昭彦

    第84回 日本動物学会  2013.9.26 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • マウス骨髄細胞培養系におけるTNF-a による破骨細胞形成と骨髄間様系細胞によるその抑制効果

    藤田洋史, 小渕浩嗣, 内海耕慥, 佐々木順造, 大内淑代

    第86回 日本生化学大会  2013.9.11 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • ALA投与によるプロトポルフィリンIX蓄積におけるABCトランスポーターABCG2の役割とミトコンドリア局在

    小渕浩嗣, 守屋康子, 荻野哲也, 藤田洋史, 井上啓史, 執印太郎, 保田立二, 内海耕慥, 内海俊彦

    第3回 ポルフィリンーALA学会年会  2013.4.27 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Acetyl-L-carnitine suppresses thyroid hormone-induced and spontaneous anuran tadpole tail shortening

    2013 

     More details

  • 5−アミノレブリン酸によるプロトポルフィリンIX蓄積におけるABCG2のミトコンドリア局在と機能

    小渕浩嗣, 藤田洋史

    第85回 日本生化学会大会  2012.9 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • ABCG2のミトコンドリア局在とプロトポルフィリンIX蓄積の制御

    小渕浩嗣

    第53回 日本生化学会 中国・四国支部例会  2012.5 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Mitochondrial localization of ABC transporter ABCG2 and its function in 5-aminolevulinic acid-mediated protoporphyrin IX accumulation.

    2012 

     More details

  • 骨髄間様系幹細胞のosteogenic cultureによる細胞死仲介性石灰化と活性酸素の関与

    藤田洋史, 小渕浩嗣, 内海耕慥, 佐々木順造, 大内淑代

    第85回 日本生化学会大会  2012 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • がん細胞のポルフィリン蓄積におけるABCG2の役割 International conference

    小渕浩嗣

    第84回日本生化学会大会  2011.9 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • がん細胞のプロトポルフィリンIX蓄積におけるABCG2の役割

    小渕浩嗣

    第52回日本生化学会中国四国支部例会  2011 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 志賀毒素のアポトーシスにおけるPKC-deltaの役割

    小渕浩嗣

    題51回 日本生化学会 中国四国支部例会  2010 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • アポトーシスにおける活性型カスパーゼ3と細胞骨格構造との結合

    藤田洋史

    第82回日本生化学会大会  2009 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • Etoposideによるアポトーシスにおける活性型カスパーゼ3の 細胞内局在-特に細胞骨格構造との結合について

    藤田洋史

    第50回日本生化学会 中国・四国支部例会  2009 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 志賀毒素のアポトーシスにおけるカスパーゼを介したミトコンドリア機能障害のフィードバック増幅

    小渕浩嗣

    第82回日本生化学会大会  2009 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 食餌性ポリフェノールによる志賀毒素誘導の腎上皮細胞アポトーシスの抑制

    小渕浩嗣

    Biochemistry and molecular biology 2008  2008 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 志賀毒素による腎上皮細胞のアポトーシスにおけるProtein kinase C-deltaの関与

    小渕浩嗣

    Biochemistry and Molecular Biology 2007  2007 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 志賀毒素誘導のアポトーシスにおけるPKC-deltaの活性化とロットレリンの効果

    小渕浩嗣

    第48回 日本生化学会中国四国支部例会  2007 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Mechanism of A23187-induced apoptosis in HL-60 cells: dependency on mitochondrial permeability transition but not NADPH oxidase International conference

    小渕浩嗣

    第20回 国際生化学・分子生物学会議 (20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress)  2006 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • A23187誘導のアポトーシスにおける活性酸素生成とミトコンドリア膜透過性遷移(MPT)の関与

    小渕浩嗣

    第17回中国四国生体ラジカル研究会  2006 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Involvement of Bax translocation in Shiga toxin-induced apoptosis

    小渕浩嗣

    第28回 日本分子生物学会年会  2005 

     More details

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • 志賀毒素はBax活性化によりアポトーシスを誘導する

    小渕浩嗣

    第78回 日本生化学会大会  2005 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 志賀毒素はJNK依存的Bcl-2リン酸化を介してアポトーシスを誘導する

    小渕浩嗣

    第77回 日本細菌学会総会  2004 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 志賀毒素誘導の腎上皮様Vero細胞のアポトーシスにおけるAktの関与

    小渕浩嗣

    第77回 日本生化学会大会  2004 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 志賀毒素のアポトーシス誘導機構におけるJNKの関与 (ワークショップ)

    第76回 日本細菌学会総会  2003 

     More details

  • 志賀毒素誘導アポトーシスにおけるJNK活性化の意義

    小渕浩嗣

    第44回 日本生化学会中四国支部例会  2003 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Requirement for ceramide-mediated JNK pathway in Shiga toxin-induced apoptosis

    小渕浩嗣

    第76回 日本生化学会大会  2003 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 六価クロムによるCHO細胞のアポトーシス誘導:特に初期機構の解析

    小渕浩嗣

    日本過酸化脂質・フリーラジカル学会第26回大会  2002 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 志賀毒素によるJNKを介したアポトーシス誘導

    小渕浩嗣

    第75回 日本生化学会大会  2002 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • コハク酸エステルによるHL-60細胞のアポトーシスとその初期反応

    小渕浩嗣

    第75回 日本生化学会大会  2002 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 志賀毒素誘導のアポトーシスにおけるセラミドの関与

    小渕浩嗣

    第75回 日本細菌学会総会  2002 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 志賀毒素のアポトーシス誘導機構におけるセラミドの関与

    小渕浩嗣

    第43回 日本生化学会中四国支部例会  2002 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • Involvement of ceramide in Shiga toxin-induced apoptotic cell death International conference

    小渕浩嗣

    Gordon Conference Glycolipid and Sphingolipid Biology  2002 

     More details

    Language:English   Presentation type:Oral presentation (general)  

    researchmap

  • 高度不飽和脂肪酸によるHL-60細胞のアポトーシスと脂質過酸化の関与

    小渕浩嗣

    第74回 日本生化学会大会  2001 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • カテプシンBによるベロ毒素誘導アポトーシスの制御

    小渕浩嗣

    第74回 日本細菌学会総会  2001 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • コレステリルサクシネートによるHL-60細胞のアポトーシス誘導機構

    小渕浩嗣

    第74回 日本生化学会大会  2001 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • メバスタチンによるHL-60細胞のアポトーシス誘導とその機構

    小渕浩嗣

    第74回 日本生化学会大会  2001 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 志賀毒素誘導のアポトーシスにおけるセラミド産生とJNK活性化の関与

    小渕浩嗣

    第74回 日本生化学会大会  2001 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

  • 高度不飽和脂肪酸によるHL60細胞のアポトーシスとその機構

    小渕浩嗣

    第73回 日本生化学会大会  2000 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ベロ毒素誘導アポトーシスにおけるカテプシンの関与

    小渕浩嗣

    第73回 日本細菌学会総会  2000 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ベロ毒素誘導アポトーシスにおけるカテプシンBの関与

    小渕浩嗣

    第73回 日本生化学会大会  2000 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ミトコンドリアMPTのカルニチンによる抑制とアポトーシスへの関与

    小渕浩嗣

    第73回 日本生化学会大会  2000 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ベロ毒素によるアポトーシス誘導機構の解析

    小渕浩嗣

    第72回 日本生化学会大会  1999 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ベロ毒素誘導アポトーシスの機構解析

    小渕浩嗣

    日本過酸化脂質・フリーラジカル学会第23回大会  1999 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • ベロ毒素のアポトーシス誘導機構の解析

    小渕浩嗣

    第72回 日本細菌学会総会  1999 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

▼display all

Industrial property rights

  • 修飾RNAを含有する分子集合体及びそれを用いたRNA送達システム

    大槻高史, 小関英一, 小渕浩嗣, 松浦栄次

     More details

    Application no:特願PTC/JP2015/081352  Date applied:2016.5.12

    WO2016/072500 A1

    researchmap

  • ポリ乳酸修飾RNAを有する分子集合体及びそれを用いたRNA送達システム

    大槻高史, 小関英一, 小渕浩嗣, 松浦栄次

     More details

    Application no:特願2014-227811  Date applied:2014.11.8

    researchmap

Awards

  • 中四国支部奨励賞

    2002.7   日本生化学会   志賀毒素のアポトーシス誘導機構におけるセラミドの関与

    小渕浩嗣

     More details

    Country:Japan

    researchmap

Research Projects

  • 骨変性部位の目印「dig-hereシグナル」を認識する破骨細胞特異的受容体の探索

    Grant number:22K09305  2022.04 - 2025.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    藤田 洋史, 小渕 浩嗣

      More details

    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    researchmap

  • Analysis of cell death induction and antitumor effect by suppression of the expression of ABC transporter

    Grant number:21K07124  2021.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    小渕 浩嗣, 藤田 洋史

      More details

    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    ATP-binding cassette transporter G2 (ABCG2)は、様々な薬剤や生理活性物質を体外に排出する生体バリア機能を担う輸送体である。代表者はABCG2機能阻害の手段として、siRNAによるABCG2遺伝子ノックダウンが、各種のがん細胞で増殖抑制や細胞死が誘導されることを見出し、その細胞死シグナル伝達機構やABCトランスポーターの新たな生理的意義を明らかにすることを目的としている。
    初年度となる令和3年度は、種々のがん細胞株における各種ABCトランスポーターの発現抑制と細胞生存率の解析を中心に行った。この解析の目的は、ABCG2と同様に、siRNAによるタンパク質発現抑制によって細胞死を誘導するABCトランスポーターの存在を明らかにすることである。
    ABCG2遺伝子の他に、ATP-binding cassette subfamily B Member 1(ABCB1,別名:P-Glycoprotein 1)、Multidrug resistance-associated protein 1(ABCC1)など、主として薬剤や生理活性物質を細胞外へ排出するABCトランスポーターについて検討した。各種siRNAの細胞内導入には市販トランスフェクション試薬を使用し、各遺伝子ノックダウン条件の最適化を行い、最も毒性の低い条件でノックダウンを施行した。膵臓がんをはじめ、様々な種類のヒトがん培養細胞株における各遺伝子ノックダウンによる影響は、それらの細胞生存率をMTT法により経時的に測定することで評価した。
    結果として、ABCG2以外でも細胞生存率を有意に低下させるABCトランスポーター遺伝子の存在を認めた。その割合はABCG2遺伝子抑制と比較すると低いものであったが、何らかの共通点が存在する可能性も否定できない。この点については、今後の課題として解析を試みる予定である。

    researchmap

  • 深部・転移がんへのRadio-induced photodynamic (RIPD) - Theranosticsを実現する89Zr標識・抗体担持生分解性キャリアの開発

    2019.04 - 2022.03

    日本医療研究開発機構 次世代がん医療創生研究事業  次世代がん医療創生研究事業

    松浦栄次、大槻高史、小渕浩嗣、宇治広隆

      More details

    Authorship:Coinvestigator(s) 

    researchmap

  • The role of pattern recognition receptors in osteoclast differentiation and function

    Grant number:19K09625  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Fujita Hirofumi

      More details

    Authorship:Coinvestigator(s) 

    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    How osteoclasts recognize old bone remains to be elucidated. We focused on pattern recognition receptors and found that some pattern recognition receptors are specifically expressed on osteoclasts. In this study, we aimed to identify old bone recognition molecules from these pattern recognition receptors. We then generated some knockout mice of candidate molecules in mice by genome editing. Contrary to expectations, disruption of the pattern recognition receptor A gene strongly promoted osteoclast differentiation. This result showed that gene A might contribute to the recognition of new bone.

    researchmap

  • 深部・転移がんへのRadio-induced photodynamic (RIPD) - Theranosticsを実現する89Zr標識・抗体担持生分解性キャリアの開発

    2016.06 - 2019.03

    日本医療研究開発機構  次世代がん医療創生研究事業 

    松浦栄次、大槻高史、小渕浩嗣、宇治広隆

      More details

    Grant type:Competitive

    researchmap

  • Development of a novel radiological-photo-therapy essential for Theranostics targeting deep lesions

    Grant number:16K15582  2016.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    MATSUURA Eiji, KOBUCHI Hirotsugu, TAKENAKA Fumiaki, AKEHI Masaru

      More details

    Grant amount:\3510000 ( Direct expense: \2700000 、 Indirect expense:\810000 )

    Photodynamic therapy (PDT) using a photosensitizer capable of inducing cytotoxicity by light irradiation is effective only for the lesion closed to the body surface, due to low permeability of the near infrared light, and is ineffective for therapy targeting the deep tissues. To solve the problem, we studied using Cherenkov light generated by β+-ray emission of a PET nuclide as a internal light source. We have given the PDT effect by Cherenkov light to the innovative "Theranostics (a treatment and diagnostic enforcement) technology" consisting of the specific antibody/Zr-89 labeled-drug delivery system (DDS) that we have ever established. We were newly able to verify some of the new basic technologies to overcome the problem (limit) of the PDT effect.

    researchmap

  • 炎症性サイトカインによる破骨細胞分化を制御するグルタチオン応答性タンパク質の同定

    2015.04 - 2017.03

    日本学術振興会  基盤研究(C)基金 

    藤田洋史

      More details

    Grant type:Competitive

    researchmap

  • 動脈硬化性疾患の早期診断を可能にする分子イメージング・リピドーム解析技術の構築

    2014.04 - 2017.03

    日本学術振興会  基盤研究(A)補助金 

    松浦栄次

      More details

    Grant type:Competitive

    researchmap

  • 骨髄間葉系幹細胞が分泌する新規破骨細胞分化調節分子の同定

    2012.04 - 2014.03

    日本学術振興会  基盤研究(C)基金 

    藤田洋史

      More details

    Grant type:Competitive

    researchmap

  • ミトコンドリア新生の促進因子のスクリーニングと細胞機能亢進の解析

    2011.04 - 2013.03

    日本学術振興会  挑戦的萌芽研究 基金 

    小渕浩嗣

      More details

    Authorship:Principal investigator  Grant type:Competitive

    researchmap

▼display all

Other research activities

  • GTP結合タンパク質に関する研究

    2018.04

  • 癌の光線動力学的診断・治療法(PDD・PDT)に関する研究

    2018.04

  • 骨髄間葉系幹細胞が分泌する新規破骨細胞分化調節分子の同定

    2018.04

  • ミリストイル化タンパク質に関する研究

    2018.04

 

Class subject in charge

  • Medical Biology (2025academic year) special  - その他

  • Biochemistry (2025academic year) Concentration  - その他

  • Mystery of Life 2 (2025academic year) Third semester  - 月7~8

  • Practicals: Organ Generation and Biomedical Engineering (2025academic year) special  - その他

  • Research Projects: Organ Generation and Biomedical Engineering (2025academic year) special  - その他

  • 医学セミナー(チュートリアル) (2024academic year) 第1学期  - 火曜日、 2,3原

  • Medical Biology (2024academic year) special  - その他

  • 医学研究インターンシップ (2024academic year) 特別

  • 基礎病態演習 (2024academic year) 特別

  • Biochemistry (2024academic year) special  - その他

  • Biochemistry (2024academic year) Concentration  - その他

  • Practice in Biochemistry (2024academic year) special  - その他

  • Mystery of Life 2 (2024academic year) Third semester  - 火1~2

  • 研究方法論基礎 (2024academic year) 特別

  • Practicals:Cell Chemistry (2024academic year) special  - その他

  • Research Projects:Cell Chemistry (2024academic year) special  - その他

  • Research Projects and Practicals: Cell Chemistry I (2024academic year) special  - その他

  • Lecture and Research Projects: Cell Chemistry I (2024academic year) special  - その他

  • Research Projects and Practicals:Cell Chemistry II (2024academic year) special  - その他

  • Lecture and Research Projects: Cell Chemistry II (2024academic year) special  - その他

  • Biochemistry (2023academic year) special  - その他

  • Biochemistry (2023academic year) Concentration  - その他

  • Practice in Biochemistry (2023academic year) special  - その他

  • Practicals:Cell Chemistry (2023academic year) special  - その他

  • Research Projects:Cell Chemistry (2023academic year) special  - その他

  • Research Projects and Practicals: Cell Chemistry I (2023academic year) special  - その他

  • Lecture and Research Projects: Cell Chemistry I (2023academic year) special  - その他

  • Research Projects and Practicals:Cell Chemistry II (2023academic year) special  - その他

  • Lecture and Research Projects: Cell Chemistry II (2023academic year) special  - その他

  • Biochemistry (2022academic year) special  - その他

  • Biochemistry (2022academic year) Concentration  - その他

  • Practice in Biochemistry and Molecular Biology (2022academic year) special  - その他

  • Practice in Biochemistry (2022academic year) special  - その他

  • Research Projects and Practicals: Cell Chemistry I (2022academic year) special  - その他

  • Lecture and Research Projects: Cell Chemistry I (2022academic year) special  - その他

  • Research Projects and Practicals:Cell Chemistry II (2022academic year) special  - その他

  • Lecture and Research Projects: Cell Chemistry II (2022academic year) special  - その他

  • Biochemistry (2021academic year) special  - その他

  • Biochemistry (2021academic year) Concentration  - その他

  • Practice in Biochemistry (2021academic year) special  - その他

  • Research Projects and Practicals: Cell Chemistry I (2021academic year) special  - その他

  • Lecture and Research Projects: Cell Chemistry I (2021academic year) special  - その他

  • Research Projects and Practicals:Cell Chemistry II (2021academic year) special  - その他

  • Lecture and Research Projects: Cell Chemistry II (2021academic year) special  - その他

  • Biochemistry (2020academic year) special  - その他

  • Biochemistry (2020academic year) Concentration  - その他

  • Practice in Biochemistry and Molecular Biology (2020academic year) special  - その他

  • Research Projects and Practicals: Cell Chemistry I (2020academic year) special  - その他

  • Lecture and Research Projects: Cell Chemistry I (2020academic year) special  - その他

  • Research Projects and Practicals:Cell Chemistry II (2020academic year) special  - その他

  • Lecture and Research Projects: Cell Chemistry II (2020academic year) special  - その他

▼display all