Updated on 2025/06/06

写真a

 
HYOUDOU Kiwamu
 
Organization
Scheduled update Associate Professor
Position
Associate Professor
External link

Degree

  • 博士(農学) ( 京都大学 )

Education

  • Kyoto University   農学研究科   応用生物科学専攻

    2011.4 - 2014.3

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    Notes: 博士課程

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  • 京都大学大学院   農学研究科   応用生物科学専攻

    2009.4 - 2011.3

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    Notes: 修士課程

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  • Kyoto University   農学部   資源生物科学科

    2005.4 - 2009.3

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Research History

  • Okayama University   Institute of Plant Science and Resources   Associate Professor

    2020.4

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  • Okayama University   Institute of Plant Science and Resources   Assistant Professor

    2015.4 - 2020.3

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Papers

  • Evidence for the replication of a plant rhabdovirus in its arthropod mite vector. International journal

    Hideki Kondo, Miki Fujita, Paul Telengech, Kazuyuki Maruyam, Kiwamu Hyodo, Aline Daniele Tassi, Ronald Ochoa, Ida Bagus Andika, Nobuhiro Suzuki

    Virus research   351   199522 - 199522   2025.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    Transmission of plant viruses that replicate in the insect vector is known as persistent-propagative manner. However, it remains unclear whether such virus-vector relationships also occur between plant viruses and other biological vectors such as arthropod mites. In this study, we investigated the possible replication of orchid fleck virus (OFV), a segmented plant rhabdovirus, within its mite vector (Brevipalpus californicus s.l.) using quantitative RT-qPCR, western blotting and next-generation sequencing. Time-course RT-qPCR and western blot analyses showed an increasing OFV accumulation pattern in mites after virus acquisition. Since OFV genome expression requires the transcription of polyadenylated mRNAs, polyadenylated RNA fractions extracted from the viruliferous mite samples and OFV-infected plant leaves were used for RNA-seq analysis. In the mite and plant datasets, a large number of sequence reads were aligned to genomic regions of OFV RNA1 and RNA2 corresponding to transcribed viral gene mRNAs. This includes the short polyadenylated transcripts originating from the leader and trailer regions at the ends of the viral genome, which are believed to play a crucial role in viral transcription/replication. In contrast, a low number of reads were mapped to the non-transcribed regions (gene junctions). These results strongly suggested that OFV gene expression occurs both in mites and plants. Additionally, deep sequencing revealed the accumulation of OFV-derived small RNAs in mites, although their size profiles differ from those found in plants. Taken together, our results indicated that OFV replicates within a mite vector and is targeted by the RNA-silencing mechanism.

    DOI: 10.1016/j.virusres.2024.199522

    PubMed

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  • SHORT AND CROOKED AWN, Encoding an Epigenetic Regulator EMF1, Promotes Barley Awn Development.

    Koki Nakamura, Yuichi Kikuchi, Mizuho Shiraga, Toshihisa Kotake, Kiwamu Hyodo, Shin Taketa, Yoko Ikeda

    Plant & cell physiology   2024.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    The awn is a bristle-like extension from the lemma of grass spikelets. In barley, the predominant cultivars possess long awns that contribute to grain yield and quality through photosynthesis. Barley is a useful cereal crop to investigate the mechanism of awn development as various awn morphological mutants are available. Here, we identified the gene causative of the short and crooked awn (sca) mutant, which exhibits a short and curved awn phenotype. Intercrossing experiments revealed that the sca mutant induced in the Japanese cultivar (cv.) 'Akashinriki' is allelic to independently isolated moderately short-awn mutant breviaristatum-a (ari-a). Map-based cloning and sequencing revealed that SCA encodes the Polycomb group-associated protein EMBRYONIC FLOWER 1 (EMF1). We found that SCA affects awn development through the promotion of cell proliferation, elongation, and cell wall synthesis. RNA sequencing of cv. Bowman (BW) backcross-derived near-isogenic lines of sca and ari-a6 alleles showed that SCA is directly or indirectly involved in promoting the expression of genes related to awn development. Additionally, SCA represses various transcription factors essential for floral organ development and plant architecture, such as MADS-box and KNOX1 genes. Notably, the repression of the C-class MADS-box gene HvMADS58 by SCA in awns is associated with the accumulation of the repressive histone modification H3K27me3. These findings highlight the potential role of SCA-mediated gene regulation, including histone modification, as a novel pathway in barley awn development.

    DOI: 10.1093/pcp/pcae150

    PubMed

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  • Replication of single viruses across the kingdoms, Fungi, Plantae, and Animalia. International journal

    Paul Telengech, Kiwamu Hyodo, Hiroaki Ichikawa, Ryusei Kuwata, Hideki Kondo, Nobuhiro Suzuki

    Proceedings of the National Academy of Sciences of the United States of America   121 ( 25 )   e2318150121   2024.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    It is extremely rare that a single virus crosses host barriers across multiple kingdoms. Based on phylogenetic and paleovirological analyses, it has previously been hypothesized that single members of the family Partitiviridae could cross multiple kingdoms. Partitiviridae accommodates members characterized by their simple bisegmented double-stranded RNA genome; asymptomatic infections of host organisms; the absence of an extracellular route for entry in nature; and collectively broad host range. Herein, we show the replicability of single fungal partitiviruses in three kingdoms of host organisms: Fungi, Plantae, and Animalia. Betapartitiviruses of the phytopathogenic fungusRosellinia necatrix could replicate in protoplasts of the carrot (Daucus carota), Nicotiana benthamiana and Nicotiana tabacum, in some cases reaching a level detectable by agarose gel electrophoresis. Moreover, betapartitiviruses showed more robust replication than the tested alphapartitiviruses. One of the fungal betapartitiviruses, RnPV18, could persistently and stably infect carrot plants regenerated from virion-transfected protoplasts. Both alpha- and betapartitiviruses, although with different host preference, could replicate in two insect cell lines derived from the fall armyworm Spodoptera frugiperda and the fruit fly Drosophila melanogaster. Our results indicate the replicability of single partitiviruses in members of three kingdoms and provide insights into virus adaptation, host jumping, and evolution.

    DOI: 10.1073/pnas.2318150121

    PubMed

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  • Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan

    Hideki Kondo, Hitomi Sugahara, Miki Fujita, Kiwamu Hyodo, Ida Bagus Andika, Hiroshi Hisano, Nobuhiro Suzuki

    Pathogens   12 ( 3 )   358 - 358   2023.2

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    Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Many aphid-borne viruses are important pathogens that affect wheat crops worldwide. An aphid-transmitted closterovirus named wheat yellow leaf virus (WYLV) was found to have infected wheat plants in Japan in the 1970s; however, since then, its viral genome sequence and occurrence in the field have not been investigated. We observed yellowing leaves in the 2018/2019 winter wheat-growing season in an experimental field in Japan where WYLV was detected five decades ago. A virome analysis of those yellow leaf samples lead to the discovery of a closterovirus together with a luteovirus (barley yellow dwarf virus PAV variant IIIa). The complete genomic sequence of this closterovirus, named wheat closterovirus 1 isolate WL19a (WhCV1-WL19a), consisted of 15,452 nucleotides harboring nine open reading frames. Additionally, we identified another WhCV1 isolate, WL20, in a wheat sample from the winter wheat-growing season of 2019/2020. A transmission test indicated that WhCV1-WL20 was able to form typical filamentous particles and transmissible by oat bird-cherry aphid (Rhopalosiphum pad). Sequence and phylogenetic analyses showed that WhCV1 was distantly related to members of the genus Closterovirus (family Closteroviridae), suggesting that the virus represents a novel species in the genus. Furthermore, the characterization of WhCV1-WL19a-derived small RNAs using high-throughput sequencing revealed highly abundant 22-nt-class small RNAs potentially derived from the 3′-terminal end of the WhCV1 negative-strand genomic RNA, indicating that this terminal end of the WhCV1 genome is likely particularly targeted for the synthesis of viral small RNAs in wheat plants. Our results provide further knowledge on closterovirus diversity and pathogenicity and suggest that the impact of WhCV1 on wheat production warrants further investigations.

    DOI: 10.3390/pathogens12030358

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  • Identification and characterization of host factors involved in plant RNA virus replication

    Kiwamu Hyodo

    JOURNAL OF GENERAL PLANT PATHOLOGY   87 ( 6 )   415 - 417   2021.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10327-021-01022-9

    Web of Science

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Books

MISC

  • Characterization of a closterovirus from wheat plants with yellowing leaf symptoms in Japan

    近藤秀樹, 兵頭究, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2024   2024

  • Replication of single partitiviruses in organisms across three kingdoms: Fungi, Plantae and Animalia

    TELENGECH Paul Kipkemboi, HYODO Kiwamu, ICHIKAWA Hiroaki, KONDO Hideki, SUZUKI Nobuhiro

    日本植物病理学会大会プログラム・講演要旨予稿集   2023   2023

  • Highjack of a host proviral vacuolar-type H+-ATPase by dianthoviruses

    兵頭究, 首藤丘宇, 近藤秀樹, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • Virome analysis of aphid populations that infest the barley field

    近藤秀樹, 藤田美貴, 久野裕, 兵頭究, 鈴木信弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021

  • Identification and characterization of host factors involved in plant RNA virus replication

    HYODO K.

    Japanese Journal of Phytopathology   87 ( 3 )   131 - 131   2021

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    Language:Japanese   Publisher:The Phytopathological Society of Japan  

    DOI: 10.3186/jjphytopath.87.131

    CiNii Article

    J-GLOBAL

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Awards

  • 学術奨励賞

    2021   日本植物病理学会  

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Research Projects

  • 作物超個体における根圏RNAウイルス叢の実体解明とその生態学的役割

    Grant number:23H02214  2023.04 - 2027.03

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    近藤 秀樹, 久野 裕, 兵頭 究, 鈴木 信弘

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    Grant amount:\18590000 ( Direct expense: \14300000 、 Indirect expense:\4290000 )

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  • 作物超個体における根圏RNAウイルス叢の実体解明とその生態学的役割

    Grant number:23K26907  2023.04 - 2027.03

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    近藤 秀樹, 久野 裕, 兵頭 究, 鈴木 信弘

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    Grant amount:\18590000 ( Direct expense: \14300000 、 Indirect expense:\4290000 )

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  • 実験室進化から紐解く抗ウイルス免疫打破株の出現と耐性機構

    Grant number:22K19183  2022.06 - 2025.03

    日本学術振興会  科学研究費助成事業 挑戦的研究(萌芽)  挑戦的研究(萌芽)

    兵頭 究

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    Grant amount:\6370000 ( Direct expense: \4900000 、 Indirect expense:\1470000 )

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  • Frontier in mycoimmunity research and its new developments in phytopathology

    Grant number:21H04727  2021.04 - 2025.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    鈴木 信弘, 近藤 秀樹, 兵頭 究

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    Grant amount:\41600000 ( Direct expense: \32000000 、 Indirect expense:\9600000 )

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  • MAPKカスケードを巡る植物-ウイルス間相互作用の解明

    Grant number:21H02198  2021.04 - 2024.03

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    兵頭 究

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    Grant amount:\17550000 ( Direct expense: \13500000 、 Indirect expense:\4050000 )

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Class subject in charge

  • Seminar in Molecular Virology (2024academic year) Prophase  - その他

  • Seminar in Molecular Virology (2024academic year) Late  - その他

  • Seminar in Virology (2024academic year) Year-round  - その他

  • Plant Virology (2024academic year) 1st semester  - 木3,木4

  • Topics in Plant-Virus Interactions (2024academic year) Late  - その他

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