Faculty Profiles - ONO Mitsuaki

MISC - ONO Mitsuaki

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抜歯窩肉芽組織由来間葉系幹細胞の同定

中島隆, 大野充昭, 園山亘, 笈田育尚, ハラ・エミリオ・サトシ, 窪木拓男

日本骨代謝学会学術集会プログラム抄録集 29回 184 - 184 2011.7

BMP-2は骨髄腔内では骨形成を促進しない

笈田育尚, 大野充昭, 園山亘, Hara Emilio, 窪木拓男

日本骨代謝学会学術集会プログラム抄録集 29回 234 - 234 2011.7

Simvastatin Induces the Odontogenic Differentiation of Human Dental Pulp Stem Cells (DPSCs)

OKAMOTO Y, SONOYAMA W, ONO M, AKIYAMA K, FUJISAWA T, OSHIMA M, TSUCHIMOTO Y, MATSUKA Y, KUBOKI T

1 ( 118 ) 78 - 78 2010.8

ヘパリン結合活性を増強した大腸菌由来改良型BMP-2を用いた骨形成能を有する口腔インプラントの開発

笈田育尚, 下野賢吾, 大野充昭, 完山学, 窪木拓男

日本口腔インプラント学会誌 23 ( 特別号 ) 150 - 150 2010.8

WISP-1/CCN4はBMP-2の機能を制御することで骨形成を促進する

大野充昭, 園山亘, 窪木拓男, ヤング・マリアン

日本骨代謝学会学術集会プログラム抄録集 28回 163 - 163 2010.7

歯周病原性細菌に対する歯科表面滑沢硬化材の影響

熊田愛, 上原淳二, 大野充昭, 松香芳三, 窪木拓男

日本補綴歯科学会誌 1 ( 1 ) E2 - E2 2009.1

bFGF局所投与における顎骨骨質改善ウサギ下顎骨モデルにおける組織学的・細胞生物学的検討

大島正充, 園山亘, 大野充昭, 下野賢吾, 暈貴行, 新川重彦, 松香芳三, 窪木拓男

日本骨代謝学会学術集会プログラム抄録集 26回 202 - 202 2008.10

bFGF局所投与における顎骨骨質改善ウサギモデルにおける組織学的・細胞生物学的検討

大島正充, 園山亘, 大野充昭, 下野賢吾, 暈貴行, 岡本洋介, 土本洋平, 松香芳三, 窪木拓男

Inflammation and Regeneration 28 ( 4 ) 335 - 335 2008.7

bFGF局所投与効果の組織学的ならびに骨形態学的検討インプラント埋入部の骨質改善に向けて

大島正充, 園山亘, 大野充昭, 下野賢吾, 窪木拓男

日本口腔インプラント学会誌 21 ( 1 ) 199 - 200 2008.3

骨再生における結合組織成長因子CCN2/CTGFの効果多機能成長因子を用いた新たな骨再生療法確立に向けて

大野充昭, 園山亘, 秋山謙太郎, 大島正充, 窪木拓男

日本口腔インプラント学会誌 21 ( 1 ) 78 - 78 2008.3

CCN family 2/connective tissue growth factor (CCN2/CTGF) stimulates proliferation and differentiation of auricular chondrocytes

T. Fujisawa, T. Hattori, M. Ono, J. Uehara, S. Kubota, T. Kuboki, M. Takigawa

Osteoarthritis and Cartilage 16 ( 7 ) 787 - 795 2008

Objectives: CCN family 2/connective tissue growth factor (CCN2/CTGF) is an atypical growth factor for growth plate chondrocytes. It plays an important role in their proliferation and differentiation in vitro, but does not stimulate hypertrophy or calcification of articular chondrocytes. We herein report for the first time that CCN2/CTGF promotes growth and differentiation of auricular chondrocytes and maintains their molecular phenotype in vitro and in vivo. Methods: Auricular chondrocytes were isolated from rabbit auricular cartilage by trypsin-collagenase treatment, and treated with human recombinant CCN2/CTGF or infected with adenovirus harboring the ccn2/ctgf gene. Cell proliferation was measured by [3H] thymidine incorporation and MTS assay, and changes in gene expression of auricular chondrocyte markers were monitored by real-time polymerase chain reaction, Northern hybridization, and histological analysis. For in vivo studies, auricular chondrocytes were cultured as pellets and implanted subcutaneously after treatment of recombinant human CCN2/CTGF. Ectopically formed cartilage was subjected to histological analysis. Cell death was monitored by in situ TUNEL analysis. Results: CCN2/CTGF stimulated proliferation, differentiation and synthesis of elastin and proteoglycans of rabbit primary auricular chondrocytes in a dose-dependent manner. CCN2/CTGF caused a 2.5-fold increase in the expression of elastin in comparison to the control, resulting in enhanced deposition of elastin fibers in a monolayer culture of auricular chondrocytes. Mineralization was not induced; in contrast, CCN2/CTGF stimulated expression of matrix gla protein which is known to impair mineralization. Furthermore, pretreatment of pellets of auricular chondrocytes with CCN2/CTGF and subcutaneous implantation significantly enhanced the growth of ectopic auricular cartilage pieces expressing phenotypic markers of auricular chondrocytes including type II and X collagen. Notably, chondrocyte apoptosis was impaired by CCN2/CTGF. Conclusions: These findings show that CCN2/CTGF may be a suitable agent for promoting differentiation and growth of auricular chondrocytes, while preventing mineralization and apoptosis, and suggests that CCN2/CTGF may be useful for the repair or reconstruction of elastic cartilage. © 2007 Osteoarthritis Research Society International.

DOI： 10.1016/j.joca.2007.11.001

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大腸菌由来・遺伝子組換BMP-2 と塩基性ゼラチンを用いた骨補填材の効果 Reviewed

下野賢吾, 大島正充, 完山学, 大野充昭, 縄稚久美子, 田畑泰彦, Walter Sebald, 洲鎌和茂, 窪木拓男

第21回日本歯科医学会総会（2008. 11.14-16．横浜） 61 ( 5 ) 513 - 513 2008