Faculty Profiles - RIKIISHI Kazuhide

写真a

RIKIISHI Kazuhide

Organization

Scheduled update Assistant Professor

Homepage

http://www.rib.okayama-u.ac.jp

External link

Degree

農学修士 ( 東北大学 )
農学博士 ( 東北大学 )

Research Interests

Plant Breeding
植物育種学

Research Areas

Environmental Science/Agriculture Science / Science in plant genetics and breeding

Education

Tohoku University

- 1987

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Tohoku University 農学研究科農学

- 1987

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Country： Japan

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Ibaraki University

- 1985

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Ibaraki University 農学部農学

- 1985

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Country： Japan

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Research History

- Assistant Professor,Institute of Plant Science and Resources,Okayama University

2004

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2004

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Professional Memberships

日本植物細胞分子生物学会

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穂発芽研究会

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日本育種学会

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Committee Memberships

穂発芽研究会幹事

2009

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Committee type：Academic society

穂発芽研究会

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Papers

Transcriptomic analysis of developing seeds in a wheat (Triticum aestivum L.) mutant RSD32 with reduced seed dormancy Reviewed

Rikiishi K, Sugimoto M, Maekawa M.

Breeding Science 71 155 - 166 2021

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1270/jsbbs.20016.
Tamyb10-D1 restores red grain color and increases grain dormancy via suppressing expression of TaLTP2.128, non-specific lipid transfer protein in wheat Reviewed

Eiko Himi, Shiho Kurihara‐Yonemoto, Fumitaka Abe, Hidekazu Takahashi, Keisuke Tanaka, Takakazu Matsuura, Masahiko Maekawa, Takuji Sasaki, Kazuhide Rikiishi

Euphytica 220 16 2024.1

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Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1007/s10681-023-03265-3

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Rice Nudix Hydrolase OsNUDX2 Sanitizes Oxidized Nucleotides Reviewed

Yuki Kondo, Kazuhide Rikiishi, Manabu Sugimoto

Antioxidants 11 ( 9 ) 1805 - 1805 2022.9

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Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Nudix hydrolase (NUDX) hydrolyzes 8-oxo-(d)GTP to reduce the levels of oxidized nucleotides in the cells. 8-oxo-(d)GTP produced by reactive oxygen species (ROS) is incorporated into DNA/RNA and mispaired with adenine, causing replicational and transcriptional errors. Here, we identified a rice OsNUDX2 gene, whose expression level was increased 15-fold under UV-C irradiation. The open reading frame of the OsNUDX2 gene, which encodes 776 amino acid residues, was cloned into Escherichia coli cells to produce the protein of 100 kDa. The recombinant protein hydrolyzed 8-oxo-dGTP, in addition to dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP), as did Arabidopsis AtNUDX1; whereas the amino acid sequence of OsNUDX2 had 18% identity with AtNUDX1. OsNUDX2 had 14% identity with barley HvNUDX12, which hydrolyzes 8-oxo-dGTP and diadenosine tetraphosphates. Suppression of the lacZ amber mutation caused by the incorporation of 8-oxo-GTP into mRNA was prevented to a significant degree when the OsNUDX2 gene was expressed in mutT-deficient E. coli cells. These results suggest that the different substrate specificity and identity among plant 8-oxo-dGTP-hydrolyzing NUDXs and OsNUDX2 reduces UV stress by sanitizing the oxidized nucleotides.

DOI： 10.3390/antiox11091805

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The Presence of the Hairy-Root-Disease-Inducing (Ri) Plasmid in Wheat Endophytic Rhizobia Explains a Pathogen Reservoir Function of Healthy Resistant Plants Reviewed International journal

Byoungwoo Kang, Taichi Maeshige, Aya Okamoto, Yui Kataoka, Shinji Yamamoto, Kazuhide Rikiishi, Akio Tani, Hiroyuki Sawada, Katsunori Suzuki

Applied and Environmental Microbiology 86 ( 17 ) 2020.8

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Language：English Publishing type：Research paper (scientific journal) Publisher：American Society for Microbiology

Crown gall and hairy root diseases exhibit very wide host-plant ranges that cover gymnosperm and dicot plants. The
<named-content content-type="genus-species">Rhizobium radiobacter</named-content>
species complex harbors causative agents of the two diseases. Recently, endophyte isolates from many plant species have been assigned to this species complex. We isolated seven endophyte strains belonging to the species complex from wheat plants and revealed their genomovar affiliations and plasmid profile. The significance of this study is the finding of the genomovar correlation between the endophytes and the known pathogens, the presence of a virulence ability in two of the seven endophyte strains, and the high ratio of the pathogenic strains in the endophyte strains. This study therefore provides convincing evidence that could unravel the mechanism that maintains pathogenic agents of this species and sporadically delivers them to susceptible plants.

DOI： 10.1128/aem.00671-20

PubMed

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Establishment of nDart1-tagged lines of Koshihikari, an elite variety of rice in Japan. Reviewed

Hideki Nishimura, Eiko Himi, Kazuhide Rikiishi, Kazuo Tsugane, Masahiko Maekawa

Breeding science 69 ( 4 ) 696 - 701 2019.12

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Language：English

To utilize a transposon-tagged mutant as a breeding material in rice, an endogenous DNA transposon, nDart1-0, was introduced into Koshihikari by successive backcrossing together with aDart1-27, an active autonomous element. The founder line for nDart1-tagged lines of Koshihikari carried nDart1-0 on chromosome 9 and transposed nDart1-12s on chromosomes 1 and 8 and nDart1-3 on chromosome 11. In nDart1-tagged lines, there were the most abnormal phenotypic mutants and many aberrant chlorophyll mutants at seedling stage. At mature stage, many semi-sterile mutants were observed. Dwarf, reduced culm number and lesion mimic mutants were also found. In total, 43.2% of the lines segregated some phenotypic mutants. Thus, the nDart1-tagged lines of Koshihikari are expected to be potentially useful for screening stress-tolerant mutants under abiotic or biotic stress conditions.

DOI： 10.1270/jsbbs.19049

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LARGE GRAIN Encodes a Putative RNA-Binding Protein that Regulates Spikelet Hull Length in Rice Reviewed

Wan-Yi Chiou, Tadafumi Kawamoto, Eiko Himi, Kazuhide Rikiishi, Manabu Sugimoto, Mika Hayashi-Tsugane, Kazuo Tsugane, Masahiko Maekawa

Plant and Cell Physiology 2019.1

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Publisher：Oxford University Press ({OUP})

DOI： 10.1093/pcp/pcz014

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ムギ種子休眠性遺伝子　～突然変異系統の解析から見えてきたこと～ Invited

力石和英

穂発芽研究会20周年記念誌 50 - 57 2019

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Authorship：Lead author Language：Japanese Publishing type：Research paper (other academic)
Light Inhibition of Shoot Regeneration Is Regulated by Endogenous Abscisic Acid Level in Calli Derived from Immature Barley Embryos Reviewed

Kazuhide Rikiishi, Takakazu Matsuura, Yoko Ikeda, Masahiko Maekawa

PLOS ONE 10 ( 12 ) e0145242 2015.12

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：PUBLIC LIBRARY SCIENCE

Shoot regeneration in calli derived from immature barley embryos is regulated by light conditions during the callus-induction period. Barley cultivars Kanto Nijo-5 (KN5) and K-3 (K3) showed lower efficiency of shoot regeneration in a 16-h photoperiod during callus-induction than those in continuous darkness, whereas shoot regeneration was enhanced in cultures under a 16-h photoperiod in Golden Promise (GP) and Lenins (LN). These cultivars were classified as photo-inhibition type (KN5 and K3) or photo-induction type (GP and LN) according to their response to light. Contents of endogenous plant hormones were determined in calli cultured under a 16-h photoperiod and continuous darkness. In photo-inhibition type, higher accumulation of abscisic acid (ABA) was detected in calli cultured under a 16-h photoperiod, whereas calli showed lower levels of endogenous ABA in continuous darkness. However, cultivars of photo-induction type showed lower levels of ABA in calli cultured under both light conditions, similarly to photo-inhibition type in continuous darkness. Exogenous ABA inhibited the callus growth and shoot regeneration independent of light conditions in all cultivars. In photo-inhibition type, lower levels of endogenous ABA induced by ABA biosynthesis inhibitor, fluridone, reduced the photo-inhibition of shoot regeneration. Expression of ABA biosynthesis gene, HvNCED1, in calli was regulated by the light conditions. Higher expression was observed in calli cultured under a 16-h photoperiod. These results indicate that ABA biosynthesis could be activated through the higher expression of HvNCED1 in a 16-h photoperiod and that the higher accumulations of ABA inhibit shoot regeneration in the photo-inhibition type cultivars.

DOI： 10.1371/journal.pone.0145242

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Seed Maturation Regulators Are Related to the Control of Seed Dormancy in Wheat (Triticum aestivum L.) Reviewed

Kazuhide Rikiishi, Masahiko Maekawa

PLOS ONE 9 ( 9 ) e107618 2014.9

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：PUBLIC LIBRARY SCIENCE

In Arabidopsis, the regulation network of the seed maturation program controls the induction of seed dormancy. Wheat EST sequences showing homology with the master regulators of seed maturation, LEAFY COTYLEDON1 (LEC1), LEC2 and FUSCA3 (FUS3), were searched from databases and designated respectively as TaL1L (LEC1-LIKE), TaL2L (LEC2-LIKE), and TaFUS3. TaL1LA, TaL2LA and TaFUS3 mainly expressed in seeds or embryos, with the expression limited to the early stages of seed development. Results show that tissue-specific and developmental-stage-dependent expressions are similar to those of seed maturation regulators in Arabidopsis. In wheat cultivars, the expression level of TaL1LA is correlated significantly with the germination index (GI) of whole seeds at 40 days after pollination (DAP) (r = -0.83**). Expression levels of TaFUS3 and TaL2LA are significantly correlated respectively with GIs at 40 DAP and 50 DAP, except for dormant cultivars. No correlation was found between the expression level of TaVP1, orthologue of ABA INSENSITIVE3 (ABI3), and seed dormancy. DELAY OF GERMINATION1 (DOG1) was identified as a quantitative trait locus (QTL) for the regulation of seed dormancy in Arabidopsis. Its promoter has RY motif, which is a target sequence of LEC2. Significant correlation was found between the expression of TaDOG1 and seed dormancy except for dormant cultivars. These results indicate that TaL1LA, TaL2LA, and TaFUS3 are wheat orthologues of seed maturation regulators. The expressions of these genes affect the level of seed dormancy. Furthermore, the pathways, which involve seed maturation regulators and TaDOG1, are important for regulating seed dormancy in wheat.

DOI： 10.1371/journal.pone.0107618

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TaABF1, ABA response element binding factor 1, is related to seed dormancy and ABA sensitivity in wheat (Triticum aestivum L.) seeds Reviewed

Kazuhide Rikiishi, Takakazu Matsuura, Masahiko Maekawa

JOURNAL OF CEREAL SCIENCE 52 ( 2 ) 236 - 238 2010.9

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD

A seed-specific bZIP type transcription factor involved in abscisic acid (ABA) signal transduction of developing wheat (Triticum aestivum L) seeds is TaABF1, ABA response element binding factor 1. The expression of TaABF1 was examined in wheat cultivars possessing different levels of seed dormancy and ABA sensitivity at 40-50 days after pollination. Zenkoujikomugi, Norin61, and OW104 were dormant cultivars showing high sensitivity to ABA. Chihokukomugi, RL4137, and Chinese Spring, all non-dormant cultivars, show low sensitivity to ABA. Tamaizumi, AUS1408, and Kitakei-1354 represent intermediate levels of seed dormancy and sensitivity to ABA. The relation between seed dormancy and ABA sensitivity is high (r = 0.89) in these cultivars. Relative amounts of transcripts of TaABF1 in embryos revealed high coefficients of correlation to seed dormancy (r = 0.78) and ABA sensitivity (r = 0.86). These results suggest that TaABF1 plays a role in the regulation of seed dormancy and ABA sensitivity in wheat. (C) 2010 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jcs.2010.05.012

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Characterization of a novel wheat (Triticum aestivum L.) mutant with reduced seed dormancy Reviewed

Kazuhide Rikiishi, Masahiko Maekawa

JOURNAL OF CEREAL SCIENCE 51 ( 3 ) 292 - 298 2010.5

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD

A novel wheat (Triticum aestivum L) mutant with reduced seed dormancy, RSD32, was isolated from a NaN(3)-treated population of a dormant cultivar, Norin61. Results show that RSD32 reduced not only the seed dormancy but also sensitivity to abscisic acid (ABA) on germination. The segregation ratio in the F(2) population of a cross that had been derived from Norin61 and RSD32 suggested that the reduced dormant phenotype was inherited as a recessive trait. At 30 days after pollination (DAP30) and later developmental stages, ABA-insensitivity of RSD32 was observed, but the seeds were sensitive to ABA at DAP20. Defects of ABA sensitivity depended upon seed development. Auxin acted synergistically for sensitivity to ABA in germination of Norin61, but the effects of auxin on ABA sensitivity were diminished in RSD32. The expression of TaABF, a bZIP class transcription factor associated with ABA signal transduction in seed, was lower in embryos of RSD32 than those of Norin61. TaDOG1 is a wheat orthologue of Arabidopsis DOG?, which is identified as a quantitative trait locus (QTL) for the regulation of seed dormancy. Moreover, RSD32 showed lower expression of TaDOG1 in embryos. Both TaABF and TaDOG1 have potential functions for regulation of wheat seed dormancy. (C) 2010 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jcs.2010.01.003

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Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos Reviewed

Kazuhide Rikiishi, Daisuke Saisho, Kazuyoshi Takeda

BREEDING SCIENCE 58 ( 2 ) 149 - 155 2008.6

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Authorship：Lead author,　Corresponding author Language：English Publisher：JAPANESE SOC BREEDING

Barley includes semi-dwarf varieties, called uzu, which are localized in parts of southwestern Japan, the Southern Korea peninsula, and coastal areas of China. The uzu phenotype possesses dark green leaves and short coleoptiles, awns, and panicles. It is controlled by a single recessive gene: uzu. Uzu results from a mutation in the brassinosteroid receptor kinase gene (HvBR11). Brassinosteroid synergistically acts with auxin on plant morphology, which is an important plant hormone for tissue culture. For this study, tissue Culture traits, including callus growth and shoot regeneration capability, were examined in F-2 populations derived from crosses between normal and uzu lines, and in isogenic lines for the uzu gene. The uzu genotype shows a lower percentage of shoot regeneration than the normal genotype in F, populations and isogenic lines. The uzu gene negatively affects shoot regeneration. No significant differences were Found In callus growth capability between uzu and normal genotypes. Uzu isogenic lines show higher sensitivity to exogenous auxin for callus initiation than normal lines, when immature embryos were incubated on media supplemented with several concentrations of 2,4-D under culture at higher temperature (25 degrees C). Tissue Culture traits of uzu might be regulated through cross-talk between brassinosteroid and auxin.

DOI： 10.1270/jsbbs.58.149

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The wheat Rc gene for red coleoptile colour codes for a transcriptional activator of late anthocyanin biosynthesis genes Reviewed

Nisar Ahmed, Masahiko Maekawa, Shigeko Utsugi, Kazuhide Rikiishi, Aftab Ahmad, Kazuhiko Noda

JOURNAL OF CEREAL SCIENCE 44 ( 1 ) 54 - 58 2006.7

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Language：English Publishing type：Research paper (scientific journal) Publisher：ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD

Genes that regulate anthocyanin pigmentation in wheat coleoptiles were investigated in three lines of wheat. When grown in the light, the wheat line Hope developed red coleoptiles, while another line, Chinese Spring (CS), expressed green coleoptiles. Another line CS(Hope7A), a chromosome substitution line that carries chromosome 7A of Hope in a CS background, also developed a red coleoptile. In the dark, none of the three lines developed colored coleoptiles. Anthocyanin biosynthesis pathway genes have been grouped into two subsets: early biosynthesis genes (EBGs), which include CHS, CHI and F3H, and late biosynthesis genes (LBGs), which include DFR, ANS and UFGT. None of these six genes was upregulated in coleoptiles grown in the dark, whereas all were activated by light in coleoptiles of Hope and CS(Hope7A). In coleoptiles of CS grown in the light, the EBGs were expressed, but the three LBGs were not. The Rc gene located on chromosome 7 appears to encode a transcription activator of LBGs involved in light-regulation of anthocyanin synthesis. (c) 2006 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jcs.2006.03.002

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ABA sensitivity in seedling of two novel mutants with reduced dormancy of a common wheat cultivar ‘Norin 61’.

Kobayashi, F, Rikiishi, K, Nakamura, C, Takumi, S

Wheat Inf. Serv. 101 4 - 7 2006

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Characteristics of alpha-amylase induced in distal half-grains of wheat Reviewed

A Hader, K Rikiishi, A Nisar, K Noda

BREEDING SCIENCE 53 ( 2 ) 119 - 124 2003.6

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Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC BREEDING

alpha-Amylase activity was measured in distal half-grains of four wheat lines, RL4137, OS21-5, Shirogane-komugi (Shirogane) and Hokkai250, after 72 h soaking at 20degreesC. The half-grains of RL4137 and Hokkai250 at the maturity stage (about 15% water content) showed a significantly (P < 0.01) higher alpha-amylase activity after 72 h soaking (3.95 mU/mg, and 2.14 mU/mg, respectively) than those of OS21-5 and Shirogane (1.05 mU/mg and 0.91 mU/mg). During grain development, 30 to 50 days postanthesis (DPA), soaking also induced the production of a-amylase in the half-grains of RL4137 after 72 h. The alpha-amylases induced consisted of heat-labile alpha-AMY2 (low pI) type of enzymes. They were similar to alpha-AMY2 which was reported to be induced in the developing pericarp in the absence of stimulus from gibberellic acid (GA), but different from heat-resistant alpha-AMY2 expressed in the aleurone tissue after stimulation by GA. It is suggested that the RL4137 aleurone tissue has a potential for the synthesis of heat-labile alpha-AMY2, although the level of induced a-amylase activity was very low compared with that of the enzyme synthesized in the embryo and endosperm at the time of germination.

DOI： 10.1270/jsbbs.53.119

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Barley lines showing prominent high green shoot regeneration in cultures derived from immature embryos Reviewed

K Rikiishi, T Matsuura, M Maekawa, K Noda, K Takeda

PLANT BREEDING 122 ( 2 ) 105 - 111 2003.4

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Language：English Publishing type：Research paper (scientific journal) Publisher：BLACKWELL VERLAG GMBH

Eighty-four cultivars of barley (Hordeum vulgare) and 95 wild strains (82 of H. spontaneum and 13 of H. agriocrithon) were surveyed for the production of callus, callus growth, and shoot regeneration in cultures derived from immature embryos. All cultivars except for 'Turkey 381', induced calli from more than 90% of embryos. On the other hand, the wild lines showed a large variation in the percentage of callus induction from 0 to 100%. Among the cultivars, those with the brittle rachis genotype, bt Bt(2), on chromosome 3H, regenerated shoots with a significantly higher percentage than the cultivars with the Bt bt(2) genotype. Green shoots were produced in a higher ratio (0.84) in the cultivars than in the wild lines (0.52). Among the lines examined, 'Lenins' regenerated shoots efficiently (90.4%) and produced the highest number of calli with green shoots per embryo (4.77) followed by 'Golden Promise' (3.15). Examination of callus growth and shoot regeneration from embryos at different developmental stages revealed that scutellum development affected the quantity and quality of callus and shoot regeneration.

DOI： 10.1046/j.1439-0523.2003.00823.x

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Genetic analysis of tissue culture traits in barley cv. 'Lenins' Reviewed

K Rikiishi, T Matsuura, M Maekawa, K Noda, K Takeda

PLANT BREEDING 122 ( 2 ) 99 - 104 2003.4

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Language：English Publishing type：Research paper (scientific journal) Publisher：BLACKWELL VERLAG GMBH

The barley cultivar 'Lenins' was found to be a genotype showing high shoot regeneration ability in cultures derived from immature embryos. Five cultivars different from 'Lenins' in shoot regeneration ability were reciprocally crossed with 'Lenins' and the inheritance of tissue culture traits was investigated. F-2 plants showed continuous distributions in callus growth and percentage of shoot regeneration, suggesting that these traits were controlled by polygenes. The F-2 population, derived from a cross between 'Lenins' and '6721', showed a monogenic segregation for the number of regenerated shoots, and the segregation ratio fitted 1 : 2 : 1. Tissue culture traits of 'Lenins' were controlled by several genes, whereas the number of regenerated shoots related to the efficiency of shoot regeneration is controlled by one major gene.

DOI： 10.1046/j.1439-0523.2003.00822.x

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Transient expression of anthocyanin in developing wheat Coleoptile by maize C1 and beta-peru regulatory genes for anthocyanin synthesis Reviewed

N Ahmed, M Maekawa, S Utsugi, E Himi, H Ablet, K Rikiishi, K Noda

BREEDING SCIENCE 53 ( 1 ) 29 - 34 2003.3

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Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC BREEDING

Maize transcription factors for the genes of anthocyanin synthesis pathway, C1/B-peru, were delivered into developing wheat coleoptiles by a particle bombardment method. Anthocyanin pigments were induced as discrete red spots and their number reached about 60 spots per coleoptile, compared to about 20 diffused blue GUS spots, which were induced by the GusA gene transferred concomitantly. Coleoptiles of seedlings collected 36 h after germination were most suitable tissue for the expression of delivered foreign genes. One to five mug of plasmid DNA for coating gold particles was sufficient for induction of anthocyanin and GUS, indicating that the transferred gene was expressed efficiently in coleoptile cells. Helium gas pressure (900, 1100, 1300 or 1500 pounds per square inch) and distances (10, 15, or 20 cm) between the stopping screen and coleoptile did not affect significantly the efficiency of gene transfer. Seedlings arranged in a circle with a 1-cm radius on a MS medium plate were targeted well by gold particles. The results showed that the wheat coleoptile was a good target tissue for transient assay of wheat genes and that C1/B-peru can be used as a reporter gene.

DOI： 10.1270/jsbbs.53.29

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Genetical Studies on Callus Growth and Plant Regeneration from Immature Embryos in Barley.

間野吉郎, 力石和英, 安田昭三

岡山大学資源生物科学研究所報告 2 ( 1 ) 43 - 53 1994.3

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A novel role for lipid-transfer protein in the control of wheat seed dormancy

氷見英子, 栗原志保, 安倍史高, 高橋秀和, 田中啓介, 松浦恭和, 前川雅彦, 力石和英

育種学研究 24 2022

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コムギ種子休眠性遺伝子～突然変異系統の解析から見えてきたこと～

力石和英

穂発芽被害ゼロをめざして穂発芽研究会20周年記念誌 50 - 57 2019

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Authorship：Lead author,　Corresponding author Language：Japanese

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Genetic analysis of a large grain mutant found in transposon-tagged lines in rice

Chiou w, K. Rikiishi, E. Himi, H. Nishimura, K. Tsugane, M. Maekawa

育種学研究 19 ( 別1 ) 55 - 55 2017

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オオムギ未熟胚培養系の植物体再分化における光制御機構のマイクロアレイ解析

力石和英, 西村秀希, 前川雅彦

育種学研究 18 ( 別2 ) 211 - 211 2016

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オオムギ未熟胚由来カルスからの植物体再分化における光制御は内生ABA含量の増加により引き起こされる

力石和英, 松浦恭和, 池田陽子, 前川雅彦

育種学研究 17 ( 別2 ) 218 - 218 2015

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オオムギ未熟胚由来カルスにおける植物体再分化と内生ホルモン含量の光制御

力石和英, 松浦恭和, 森泉, 前川雅彦

育種学研究 16 ( 別1 ) 228 - 228 2014

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Effects of seed maturation regulators on seed dormancy in wheat

K. Rikiishi, M. Maekawa

Proceedings of the 12th international wheat genetics symposium 150 - 150 2013

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Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos

Kazuhide Rikiishi, Takakazu Matsuura, Masahiko Maekawa, Kazuyoshi Takeda

BREEDING SCIENCE 58 ( 2 ) 129 - 135 2008.6

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Authorship：Lead author,　Corresponding author Language：English Publisher：JAPANESE SOC BREEDING

Callus growth and shoot regeneration were examined in Cultures of immature barley embryos incubated under various combinations of a week under a 16-h photoperiod and a week under continuous darkness. incubation in darkness for four weeks, during which calli were formed, enhanced shoot regeneration in 'K-3' and 'Kanto Nijo-5', but inhibited it in 'Lenins'. 'K-3' and 'Kanto Nijo-5', incubated in darkness during the first two weeks of callus induction, followed by two weeks of a 16-h photoperiod (D2L2), showed a higher percentage of shoot regeneration than those incubated for two weeks with a 16-h photoperiod followed by two weeks of darkness (L2D2). Nevertheless, when the light conditions, D2L2 and L2D2, were combined into the same periods of a 16-h photoperiod and continuous darkness, except for the order, light conditions affected shoot regeneration differently. The early stage of callus induction seems to be sensitive to light. The expression ratios of the auxin-responsive gene (AUX/IAA) to the cytokinin-responsive gene (WPK4) were increased in continuous darkness ill 'K-3' and 'Kanto Nijo-5'. The effects of light qualities (white, red, far-red, and blue) on callus growth and shoot regeneration were also examined. Blue light inhibited shoot regeneration, as did white light.. in 'K-3' and 'Kanto Nijo-5', and far-red light ill 'Kanto Nijo-5'. Light probably controls shoot regeneration from calli by modifying cytokinin levels and/or response; blue light signals act in photo-inhibition of shoot regeneration in immature barley embryo Culture.

DOI： 10.1270/jsbbs.58.129

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東アジアに局在するオオムギ半矮性系統”渦”の進化的・形態的・生理的な多様性解析

武田和義, 力石和英, 最相大輔

平成16年度〜平成18年度科学研究費補助金基盤研究B 研究成果報告書 1 - 110 2008

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突然変異系統を利用したコムギ穂発芽発生機構の解析

力石和英

平成16年度〜平成18年度科学研究費補助金基盤研究C 研究成果報告書 1 - 43 2007

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コムギの種子休眠性が低下した突然変異系統におけるABA感受性の解析

力石和英, 前川雅彦

育種学研究 2006

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ABA sensitivity in seedlings of novel three reduced dormancy mutants of a common wheat cultovar 'Norin61'

Kobayashi, F, Rikiishi, K, Nakamura, C, Takumi, S

Wheat Information Service 2005

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Analysis of mutants with reduced seed dormancy

Rikiishi, K, Maekawa, M

Proc. 10th Int. Symp. Pre-Harvest Sprout. in Cereals 2004

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オオムギ未熟胚由来カルスからの植物体再分化に及ぼすフィトクロムの影響

力石和英, 前川雅彦

育種学研究 2004

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コムギ除胚半切種子で誘導されるα-amylaseの特性

Hader Albet, 力石和英, Nisar Ahmed, 野田和彦

育種学研究 5 ( 2 ) 60 - 60 2003.6

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トウモロコシのアントシアニン合成系遺伝子の制御因子CIとB-peruによる発達中のコムギ幼葉鞘におけるアントシアニンの一過的発現

Ahmed Nisar, 前川雅彦, 宇都木繁子, 氷見英子, Ablet Hader, 力石和英, 野田和彦

育種学研究 5 ( 1 ) 22 - 23 2003.3

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トウモロコシのアントシアニン合成系遺伝子の転写制御因子CI B-peruを発達中のコムギ幼葉鞘にパーティクルボンバードメント法で導入した.アントシアニン色素が明確な赤い点として誘導され,その数は幼葉鞘当たり約60に達した.一方,同時に導入したGusA遺伝子では約20の滲んだ青いGUSの点が誘導された.発芽後36時間の幼葉鞘が,これら外来遺伝子の導入発現に最も良い組織であった.また1から5μgのプラスミドDNAで覆った金粒子で充分にアントシアニンやGUSの発現が誘導された.ヘリウムガス圧(900,1100,1300,1500ポンド/インチ^2)或いは停止スクリーンと幼葉鞘間の距離(10,15,20？)は遺伝子導入効率には有意に影響を与えなかった.一方,MS培地に半径1？の円状に並べた芽生えで最も良く遺伝子が導入された.これらの結果は,コムギ幼葉鞘はコムギ遺伝子の一過的発現分析に良い組織であり,またCI B-peruはレポーター遺伝子として使用できることを示した.

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Transient expression of anthocyanin in developing wheat Coleoptile by maize C1 and beta-peru regulatory genes for anthocyanin synthesis

N Ahmed, M Maekawa, S Utsugi, E Himi, H Ablet, K Rikiishi, K Noda

BREEDING SCIENCE 53 ( 1 ) 29 - 34 2003.3

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Maize transcription factors for the genes of anthocyanin synthesis pathway, C1/B-peru, were delivered into developing wheat coleoptiles by a particle bombardment method. Anthocyanin pigments were induced as discrete red spots and their number reached about 60 spots per coleoptile, compared to about 20 diffused blue GUS spots, which were induced by the GusA gene transferred concomitantly. Coleoptiles of seedlings collected 36 h after germination were most suitable tissue for the expression of delivered foreign genes. One to five mug of plasmid DNA for coating gold particles was sufficient for induction of anthocyanin and GUS, indicating that the transferred gene was expressed efficiently in coleoptile cells. Helium gas pressure (900, 1100, 1300 or 1500 pounds per square inch) and distances (10, 15, or 20 cm) between the stopping screen and coleoptile did not affect significantly the efficiency of gene transfer. Seedlings arranged in a circle with a 1-cm radius on a MS medium plate were targeted well by gold particles. The results showed that the wheat coleoptile was a good target tissue for transient assay of wheat genes and that C1/B-peru can be used as a reporter gene.

DOI： 10.1270/jsbbs.53.29

Web of Science

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種子休眠性が低下したコムギ突然変異体の解析

力石和英, 松浦恭和, 前川雅彦, 野田和彦

育種学研究 2003

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渦遺伝子がオオムギ未熟胚由来カルスからの植物体再分化に及ぼす影響

力石和英, 最相大輔, 前川雅彦, 武田和義

育種学研究 2003

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Wheat Re gene for coleoptile colour regulates the expression of DFR gene in developing coleoptile.

Ahmed, N, Utsugi, S, Himi, E, Maekawa, M, Rikiishi, K, Matsuura, T, Noda, K

Breed. Res. 4 (suppl. 1) 2002

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コムギ除胚半切種子で吸水によって誘導されるα-アミラーゼの特性

ハードルアブラビ, 力石和英, 宇都木繁子, 松浦恭和, 前川雅彦, 野田和彦

育種学研究 2002

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オオムギにおける半矮性「渦」の特性解析

武田和義, 最相大輔, 力石和英, 北野英巳

育種学研究 2002

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Light control of shoot regeneration in callus cultures derived from barley immature embryos

Rikiishi, K

Proc. 2nd Symp. Agricultural Plant Stress Research Center 2002

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高いαーアミラーゼ活性を示したコムギ除胚半切種子におけるデンプン分解酵素の解析

ハードル、アブラビ, 力石和英, 宇都木繁子, 松浦恭和, 前川雅彦, 野田和彦

育種学研究 2001

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Sugar suppression of alfa-glucosidase activity in wheat embryos

Rikiishi, K, Sugimoto, M, Maetani, M, Matsuura T, Utsugi, S, Maekawa, M, Noda, K

Proc. 9th Int. Symp. Pre-Harvest Sprouting in Creals 2001

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コムギ胚におけるα-glucosidase活性の糖による抑制

力石和英, 杉本泰俊, 前谷成生, 前川雅彦, 野田和彦

育種学研究 2001

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Aberrant segregation of 1g observed in F2 of I-102(indica type)//T-65 1g BC10 in rice.

MAEKAWA M., RIKIISHI K., MATSUURA T., UTSUGI S., NODA K.

2 ( 2 ) 256 - 256 2000.9

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インド型イネI-102//T-65 lg BC10F2に生じたlgの異常分離

前川雅彦, 力石和英, 松浦恭和, 宇都木繁子, 野田和彦

育種学研究 2000

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イネ完熟種子糊粉層におけるアントシアニン生合成に関わる転写制御因子のトランジェントアッセイ系

前川雅彦, 景山圭祐, 力石和英, 宇都木繁子, 野田和彦, 坂本亘

育種学研究 2 ( 4 ) 181 - 187 2000

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イネにおけるアントシアニン生合成の転写制御遺伝子の発現解析に必要なトランジェントアッセイ系を, 常時材料を準備できるイネ完熟種子糊粉層で確立した. まず, 岡大資生研紫米 (RIB-PI) の完熟種子を用いて糊粉層を露出するための吸水時間と温度を検討した. 露出できた糊粉層にトウモロコシのアントシアニン生合成の転写制御遺伝子, ClとB-Peruをパーティクルガンで導入したところ, アントシアニンのスポットが観察されたので, 両遺伝子を用いたトランジェントアッセイ系について至適条件を検討した. その結果, 28℃ で4時間吸水させて露出した糊粉層でアントシアニンのスポット数が最大になった. また, 導入するプラスミドの量は, ClとBPeruがともに5μgかClが5μgでB-Peruが10μgのときに, アントシアニンのスポット数が多くなった. 以上の結果から, イネの完熟種子糊粉層を用いたトランジェントアッセイとして, 28℃ で4時間吸水させて糊粉層を露出させ, C1とB-Pmをともに5μgずつ導入することによって, 安定的にアントシアニンを誘導できることが明らかとなった.

DOI： 10.1270/jsbbr.2.181

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Non-segregation of albino character in rice plants heterozygous for variegated albino

MAEKAWA M., RIKIISHI K., MATSUURA T., UTSUGI S., NODA K.

2 ( 1 ) 15 - 15 2000

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Non-segregation of albino character in rice plants heterozygous for variegated albino

MAEKAWA M., RIKIISHI K., MATSUURA T., UTSUGI S., NODA K.

2 ( 1 ) 15 - 15 2000

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Wheat seed color and expression of the genes in anthocyanin synthesis pathway

HIMI E., UTSUGI S., RIKIISHI K., MAEKAWA M., NODA K.

2 ( 1 ) 71 - 71 2000

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イネ完熟種子糊粉層におけるアントシアニン生合成に係わる転写制御遺伝子のトランジットアッセイ系

前川雅彦, 景山圭祐, 力石和英, 宇都木繁子, 野田和彦, 坂本亘

育種学研究 2 ( 4 ) 181 - 187 2000

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イネにおけるアントシアニン生合成の転写制御遺伝子の発現解析に必要なトランジェントアッセイ系を, 常時材料を準備できるイネ完熟種子糊粉層で確立した. まず, 岡大資生研紫米 (RIB-PI) の完熟種子を用いて糊粉層を露出するための吸水時間と温度を検討した. 露出できた糊粉層にトウモロコシのアントシアニン生合成の転写制御遺伝子, ClとB-Peruをパーティクルガンで導入したところ, アントシアニンのスポットが観察されたので, 両遺伝子を用いたトランジェントアッセイ系について至適条件を検討した. その結果, 28℃ で4時間吸水させて露出した糊粉層でアントシアニンのスポット数が最大になった. また, 導入するプラスミドの量は, ClとBPeruがともに5μgかClが5μgでB-Peruが10μgのときに, アントシアニンのスポット数が多くなった. 以上の結果から, イネの完熟種子糊粉層を用いたトランジェントアッセイとして, 28℃ で4時間吸水させて糊粉層を露出させ, C1とB-Pmをともに5μgずつ導入することによって, 安定的にアントシアニンを誘導できることが明らかとなった.

DOI： 10.1270/jsbbr.2.181

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イネのアントシアニン制御因子XT1とXT2による糊粉層におけるアントシアニン発現

景山圭祐, 大森拓, 前川雅彦, 力石和英, 野田和彦, 坂本亘, 本吉総男

育種学研究 1999

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イネアントシアニン転写制御遺伝子PLwの構造

大森拓, 宮崎力, 景山圭祐, 前川雅彦, 力石和英, 野田和彦, 斉藤彰, 本吉総男, 坂本亘

育種学研究 1999

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コムギ幼穂におけるexpressed Sequence Tags (EST)の大量解析

荻原保成, 藤田雅子, 川浦香奈子, 大澤智子, 村井耕二, 松岡由浩, 布藤聡, 佐藤恵美, 早川克志, 野田和彦, 宇都木繁子, 力石和英, Ahmed Nisar

育種学研究 1999

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Putative alternative splicing occurred in XT2, a transcription factor for anthocyanin pigmentation in rice.

景山圭祐, 大森拓, 前川雅彦, 力石和英, 宇都木繁子, 野田和彦, 坂本亘, 本吉総男

育種学研究 1 1999

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遠縁イネ間交雑から生じた葉緑素異常変異体における易変性の遺伝的制御

前川雅彦, 力石和英, 松浦恭和, 野田和彦

育種学研究 1999

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コムギ種子胚のアブシジン酸（ABA）感受性に関わる染色体の同定

野田和彦, 松浦恭和, 氷見英子, 力石和英, 前川雅彦

育種学研究 1999

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Geographical Differentiation and Varietal Variation in Root Differentiation Ability of Calli Derived from Mature Embryos in Local Barley Varieties.

Rikiishi Kazuhide, Takeda Kazuyoshi, Yasuda Shozo

Breeding Science 45 ( 2 ) 211 - 215 1995

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Differentiation ability of calli derived from mature embryos was surveyed using a total of 269 Iocal barley varieties collected from various countries or regions of the world. Eighty five varieties differentiated roots, though they did not regenerate shoots. The percentages of root differentiation in all tested varieties ranged from O to 100 %, and their average was 10.2 % . Varieties collected from Japan, Korea. China. Nepal, Turkey and North Africa showed a low differentiation ability (1 -5 % ), while those from South-West Asia, Europe and Ethiopia showed a high differentiation ability (13-19 % ).

DOI： 10.1270/jsbbs1951.45.211

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Geographical Differentiation and Varietal Variation in Root Differentiation Ability of Calli Derived from Mature Embryos in Local Barley Varieties.

Rikiishi Kazuhide, Takeda Kazuyoshi, Yasuda Shozo

Breeding Science 45 ( 2 ) 211 - 215 1995

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Differentiation ability of calli derived from mature embryos was surveyed using a total of 269 Iocal barley varieties collected from various countries or regions of the world. Eighty five varieties differentiated roots, though they did not regenerate shoots. The percentages of root differentiation in all tested varieties ranged from O to 100 %, and their average was 10.2 % . Varieties collected from Japan, Korea. China. Nepal, Turkey and North Africa showed a low differentiation ability (1 -5 % ), while those from South-West Asia, Europe and Ethiopia showed a high differentiation ability (13-19 % ).

DOI： 10.1270/jsbbs1951.45.211

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Geographical dfferentiation and varietal variation in root differentiation ability of calli derived from mature embryos in local barley varieties

Rikiishi Kazuhide, Takeda Kazuyoshi, Yasuda Shozo

Breeding Science 45 ( 2 ) 211 - 215 1995

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Differentiation ability of calli derived from mature embryos was surveyed using a total of 269 Iocal barley varieties collected from various countries or regions of the world. Eighty five varieties differentiated roots, though they did not regenerate shoots. The percentages of root differentiation in all tested varieties ranged from O to 100 %, and their average was 10.2 % . Varieties collected from Japan, Korea. China. Nepal, Turkey and North Africa showed a low differentiation ability (1 -5 % ), while those from South-West Asia, Europe and Ethiopia showed a high differentiation ability (13-19 % ).

DOI： 10.1270/jsbbs1951.45.211

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オオムギ近緑野生種と栽培種における未熟胚由来カルスからの再分化能の比較

力石和英

岡山大学資源生物科学研究所報告 3 ( 1 ) 54 - 62 1995

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オオムギ未熟胚培養系における植物体再分化能の遺伝解析

間野吉郎, 力石和英, 安田昭三

日本作物学会中国支部研究集録 ( 34 ) 78 - 79 1993.8

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近年、作物の育種を行うにあたり、形質転換や細胞選抜等の生物工学的手法が利用されるようになってきた。その一方で、形質転換や細胞選抜では培養細胞からの植物体再分化が困難であるなどの問題点がある。本研究では、オオムギ培養細胞からの再分化を制御し、生物工学的手法の実用化に寄与するために、植物体再分化を支配する遺伝子の遺伝様式の解析を行った。

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オオムギ栽培品種における完熟胚培養系の不定根再分化に関する地理的変異

力石和英, 安田昭三

日本作物学会中国支部研究集録 ( 33 ) 38 - 39 1992.8

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C₄-like plants derived from a cross (Atriplex rosea (C₄) x A. patula (C₃)) x A. rosea

RIKIISHI Kazuhide, OGUR0 Hitoshi, SAMEJIMA Muneaki, SUGIYAMA Tatsuo, HINATA Kokichi

Japanese Journal of Breeding 38 ( 4 ) 397 - 408 1988

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To analyse the genetic aspects of C₃ and C₄ photosynthetic systems, the photosynthetic charcacteristics of the progenies derived from a backcross, (Atriplex rosea (C₄, 2 n=18, 2x)xA.patula (C₃, 2n=36, 4x))xA, rosea, were investigated. Out of three plants derived from the backcross, two showed C4-Iike traits based on the C0₂ compensation point and δ¹³C values as well as enzyme analysis and leaf anatomy. Another plant showed C₃-like characteristics. The descendants of these plants were segregated into three discrete morphofogical types ; p-type plants which were similar to A. patula ; d-type plants which were dwarf with dark green leaves ; and r-type plants which resembled A.rosea but exhibited a highly branching habit. The isozyme patterns of the leaves of the pand d-types were similar to those of A.patula, while th.e isozyme patterns of the r-type were either similar to those of A.rosea or different from the parental patterns. Based on anatomical, photosynthetic and enzymological observations, the p- and d-types showed C₃-like traits, whereas the r-type C₄-like ones. Chromosome analysis from the root tips revealed a 2n=36 number in the p- and d-types and a 2n=18 one in the r-type. It is suggested that the expression of C₄ photosynthesis is affected by the dose of the genome of the C₄ species and that the genes related to C₄ photosynthesis tend to be inherited as a group in the interspecific hybrids.

DOI： 10.1270/jsbbs1951.38.397

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C4-like plants derived from a cross (Atriplex rosea (C4)*A. patula (C3))*A. rosea.

RIKIISHI Kazuhide, OGUR0 Hitoshi, SAMEJIMA Muneaki, SUGIYAMA Tatsuo, HINATA Kokichi

Breeding Science 38 ( 4 ) 397 - 408 1988

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To analyse the genetic aspects of C₃ and C₄ photosynthetic systems, the photosynthetic charcacteristics of the progenies derived from a backcross, (Atriplex rosea (C₄, 2 n=18, 2x)xA.patula (C₃, 2n=36, 4x))xA, rosea, were investigated. Out of three plants derived from the backcross, two showed C4-Iike traits based on the C0₂ compensation point and δ¹³C values as well as enzyme analysis and leaf anatomy. Another plant showed C₃-like characteristics. The descendants of these plants were segregated into three discrete morphofogical types ; p-type plants which were similar to A. patula ; d-type plants which were dwarf with dark green leaves ; and r-type plants which resembled A.rosea but exhibited a highly branching habit. The isozyme patterns of the leaves of the pand d-types were similar to those of A.patula, while th.e isozyme patterns of the r-type were either similar to those of A.rosea or different from the parental patterns. Based on anatomical, photosynthetic and enzymological observations, the p- and d-types showed C₃-like traits, whereas the r-type C₄-like ones. Chromosome analysis from the root tips revealed a 2n=36 number in the p- and d-types and a 2n=18 one in the r-type. It is suggested that the expression of C₄ photosynthesis is affected by the dose of the genome of the C₄ species and that the genes related to C₄ photosynthesis tend to be inherited as a group in the interspecific hybrids.

DOI： 10.1270/jsbbs1951.38.397

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On C4-Iike backcrossed progenies, obtained in (( Atriplex rosea (C4,2n) x A.patula (C3,4n)) x A. rosea) in view of CO2 compensation point and isozyme analysis

RIKIISHI K., HINATA K.

Japanese Journal of Breeding 36 ( 2 ) 178 - 179 1986

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Presentations

MutMap法を用いたコムギの種子休眠性低下突然変異rsd32の同定

力石和英, 土屋江利, 杉本学

穂発芽研究会 2024.12.10

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Event date： 2024.12.10

Language：Japanese Presentation type：Oral presentation (general)

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MutMap法によるコムギの種子休眠性低下突然変異の原因遺伝子rsd32の同定

力石和英, 土屋江利, 杉本学

種子生理生化学研究会年会 2024.11.22

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Event date： 2024.11.21 - 2024.11.22

Language：Japanese Presentation type：Oral presentation (general)

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MutMap法を用いたコムギの種子休眠性低下突然変異rsd32の同定

力石和英, 土屋江利, 杉本学

日本育種学会 2024.9.20

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Event date： 2024.9.19 - 2024.9.20

Language：Japanese Presentation type：Poster presentation

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酸化ヌクレオチド除去に関与するトマトNudix hydrolaseの探索

横山聡太郎, 力石和英, 杉本学

日本農芸化学会 2024.3.24

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Event date： 2024.3.24 - 2024.3.27

Language：Japanese Presentation type：Oral presentation (general)

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コムギ内生菌アグロバクテリウムのサイトカイニン合成と合成遺伝子クラスター

鈴木克周, 清川一矢, 山篠貴史, 力石和英

第46回日本分子生物学会年会 2023.12.6

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Event date： 2023.12.6 - 2023.12.8

Language：Japanese

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RNA-seq analysis of developing seeds in a wheat mutant RSD32 with reduced seed dormancy

Rikiishi, K, Sugimoto, M, Maekawa, M

15th International Symposium on Pre-Harvest Sprouting in Cereals 2023.10.5

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Event date： 2023.10.4 - 2023.10.6

Language：English

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酸化ヌクレオチド消去をするイネOsNUDX2の転写エラー抑制機能

杉本学, 力石和英, 近藤雄基

日本宇宙生物科学会第37回大会 2023.9.22

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Event date： 2023.9.22 - 2023.9.24

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イネアルビノ様葉緑素変異体pyl-stbの幼苗致死性に関する光応答制御機構の解析

力石和英, 小野蒼生, 前川雅彦, 杉本学

日本育種学会第142回講演会 2022.9.24

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Event date： 2022.9.23 - 2022.9.25

Language：Japanese Presentation type：Poster presentation

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酸化ヌクレオチド消去に関与するイネNudix hydrolaseの探索

近藤雄基, 力石和英, 杉本学

日本宇宙生物科学会第36回大会 2022.9.16

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Event date： 2022.9.16 - 2022.9.18

Language：Japanese Presentation type：Oral presentation (general)

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コムギ種子休眠に関わるlipid transfer protein遺伝子について

氷見英子, 栗原志保, 安倍史高, 高橋秀和, 田中啓介, 松浦恭和, 前川雅彦, 力石和英

日本育種学会第141回講演会 2022.3.20

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Event date： 2022.3.20 - 2022.3.21

Language：Japanese Presentation type：Oral presentation (general)

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コムギの根から単離したアグロバクテリア菌株NR3の特性とゲノム

鈴木克周, 清川一矢, 谷明生, 力石和英

第7回デザイン生命工学研究会 2022.3.10

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Event date： 2022.3.10

Language：Japanese Presentation type：Oral presentation (general)

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Genetic analysis of a large grain mutant found in transposon-tagged lines in rice

日本育種学会第131回講演会 2017

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RNA-seqによるコムギ種子休眠性低下突然変異系統RSD32の遺伝解析

日本育種学会第132回講演会 2017

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Genetic analysis of the large grain mutant found in transposon-tagged lines of rice

Taiwan-Japan Plant Biology 2017 2017

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概日時計遺伝子はコムギの種子休眠性を制御するのか？

第21回穂発芽研究会 2017

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オオムギ未熟胚培養系の植物体再分化における光制御機構のマイクロアレイ解析

日本育種学会第130回講演会 2016

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Microarray analysis for photo-regulation of shoot regeneration in callus cultures derived from barley immature embryos

2016

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オオムギ未熟胚由来カルスからの植物体再分化における光制御は内生ABA含量の増加により引き起こされる

日本育種学会第128回講演会 2015

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オオムギ未熟胚由来カルスにおける植物体再分化と内生ホルモン含量の光制御

日本育種学会第125回講演会 2014

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種子成熟制御因子はコムギの種子休眠性を制御するか？

日本育種学会・日本作物学会北海道談話会例会 2014

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穂発芽抵抗性白粒コムギ1-117のMFTタイプと種子休眠

第18回穂発芽研究会 2014

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Effects of seed maturation regulators on seed dormancy in wheat

第12回国際コムギ遺伝学シンポジウム 2013

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種子成熟因子がコムギ種子休眠性に及ぼす影響

日本育種学会第123回講演会 2013

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国際穂発芽シンポジウム報告

第16回穂発芽研究会 2012

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コムギにおけるLEC2様遺伝子の発現解析

第16回穂発芽研究会 2012

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Characterization of a novel wheat (Triticum aestivum L.) mutant with reduced seed dormancy

第12回国際穂発芽シンポジウム 2011

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コムギ種子発芽の光制御と休眠性

第15回穂発芽研究会 2010

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Analysis of wheat mutants in reduced seed dormancy

The 14th meeting of a society of Pre-Harvest Sprouting 2010

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Effects of seed dormancy on the light control of germination in wheat

The 15th meeting of a Society of Pre-Harvest Sprouting 2010

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突然変異系統を用いたコムギ穂発芽制御機構の解析

第14回穂発芽研究会 2010

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コムギ種子休眠性の制御に関する遺伝子の解析

日本育種学会第116回講演会 2009

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Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos

10th international barley genetics symposium 2008

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コムギの種子休眠性低下変異体におけるABA信号伝達関連遺伝子の発現解析

日本育種学会第112回講演会 2007

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コムギ種子休眠性が低下した突然変異系統におけるABA感受性の解析

日本育種学会第109回講演会 2006

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オオムギにおける形質転換

第一回理研植物科学研究センター形質転換勉強会 2006

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オオムギ未熟胚由来カルスからの植物体再分化に及ぼすフィトクロムの影響

日本育種学会 2004

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Analysis of wheat mutants with reduced seed dormancy

10th International symposium on pre-harvest sprouting in cereals 2004

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渦遺伝子がオオムギ未熟胚由来カルスからの植物体再分化に及ぼす影響

日本育種学会第１０４回講演会 2003

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種子休眠性が低下したコムギ突然変異体の解析

日本育種学会第１０３回講演会 2003

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オオムギにおける半矮性「渦」の特性解析

日本育種学会第102回講演会 2002

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Light control of shoot regeneration in callus cultures derived from barley immature embryos.

Agricultural Plant Stress Response 2 2002

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Wheat Re gene for coleoptile colour regulates the expression of DFR gene in developing coleoptile.

日本育種学会第101回講演会 2002

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コムギ除胚半切種子で吸水によって誘導されるα-アミラーゼの特性

日本育種学会第101回講演会 2002

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高いα-amylaseを示したコムギ除胚半切種子におけるデンプン分解酵素の解析

日本育種学会 2001

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Sugar suppression of alfa-glucosidase activity in wheat embryos

9th Int. Symp. on Pre-Harvest Sprouting 2001

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コムギ胚におけるα-glucosidase活性の糖による制御

日本育種学会 2001

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イネの斑入りアルビノ形質ヘテロ個体におけるアルビノ形質不分離

日本育種学会 2000

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コムギ粒色とアントシアニン合成系遺伝子の発現

日本育種学会 2000

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インド型イネ I-102//T-65 lg BC10F2に生じた異常分離

日本育種学会 2000

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コムギ種子胚のアブシジン酸（ABA）感受性に関わる染色体の同定

日本育種学会 1999

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イネのアントシアニン制御因子XT1とXT2による糊粉層におけるアントシアニン発現

日本育種学会 1999

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イネアントシアニン転写制御遺伝子Plwの構造

日本育種学会 1999

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コムギ幼穂におけるExpressed Sequence Tags (EST)の大量解析

日本育種学会 1999

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イネのアントシアニン制御因子XT2におけるalternative splicing

日本育種学会 1999

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遠縁イネ間交雑から生じた葉緑素異常変異体における易変性の遺伝的制御

日本育種学会 1999

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Research Projects

コムギ種子休眠性を誘導する新規の種子特異的制御因子の機能解明とその育種的利用

Grant number：22K05573 2022.04 - 2025.03

日本学術振興会科学研究費助成事業基盤研究(C)

力石和英

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Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

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Cereal plant transformation using endophytic agrobacteria

Grant number：21H02167 2021.04 - 2025.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

鈴木克周, 力石和英

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Grant amount：\17290000 （ Direct expense: \13300000 、 Indirect expense：\3990000 ）

主要穀物植物は単子葉植物であり、病原性アグロバクテリア（Agrobacterium tumefaciens）による感染を受けず、ムギ類に代表されるようにアグロバクテリアを用いる形質転換が難しい。本研究は植物の形質転換問題を改善することを目標としている。先ず、申請者らがコムギから単離したアグロバクテリア内生菌株を植物形質転換に汎用的に利用できるドナー株に改変加工を行う。次いで、胚性カルスの形質転換へ適用する評価試験を行ってどのような特性を持つか明らかにすることで、世界で初めて有用株化した内生細菌株を公開し、広く形質転換に活用できるように整備することが本研究の目的である。
既に申請者らがコムギから単離したアグロバクテリア（A. tumefaciens）内生菌株には、Riプラスミドを持つG７グループ菌株とTiプラスミド/Riプラスミドどちらをも持たないG１グループ菌株の計２系統がある。2021年度の実施計画は主に、それぞれの内生菌株でT-DNA輸送能力を持つが病気を生じさせない（アームレス化）ドナー菌株を作成することと、来年度本格実施する植物形質転換の準備を行うという内容である。
G１グループ内生菌株へアームレスRiプラスミド導入した菌株を作成した。G７グループ内生菌株については、Riプラスミド除去を行ったうえでアームレスRiプラスミド導入した菌株を作成した。一方、既存のTiプラスミド導入体を得ることができなかった。G７グループ内生菌株RN3の塩基配列決定によって、野生型RiプラスミドpRiRN3は長さ234,326 bpであり、１つのT-DNAを持っていることが判った。この配列情報に基づいて、T-DNA部分のみを除去するための加工用プラスミドを設計した。

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Analysis of wheat mutants in reduced seed dormancy

Grant number：16580005 2004 - 2006

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

RIKIISHI Kazuhide

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Grant amount：\3400000 （ Direct expense: \3400000 ）

In this project, wheat mutants in reduced seed dormancy were analyzed for understanding the regulations of seed dormancy and pre-harvest sprouting in wheat.
1. ABA sensitivity in RSD (Reduced Seed Dormancy) mutants
RSD mutants showed lower sensitivity to exogenous ABA and the levels of sensitivity were different among mutant lines. Seed developmental stages affected ABA sensitivity of Norin 61 and RSD mutants and the different responses had shown among mutants.
2. Genetic analysis
Reduced seed dormancy of RSD14-1 and RSD12 were dominant mutations and those of RSD16-1 and RSD32 were recessive mutations. Segregation ratio for reduced seed dormancy in F2 population derived from the cross between Norin 61 and RSD32, suggests that mutant phenotype of RSD32 is a single recessive mutation.
3. Analysis of the regulation for a-amylase expression
RSD mutants showed the higher expression of a-amylase in embryos than Norin 61. Expression levels of genes that were negative regulator of a-amylase and showed seed specific manner were determined. TaABF were expressed lower levels in three RSD mutants. Two RSD mutants also showed lower expression of TaAFP.
4. Regulation of ABA inducible genes in RSD mutants
Expression of Em genes that were induced by ABA and specifically expressed in seeds were lower level in RSD mutants, suggesting that ABA signals diminished in seed of RSD mutants. On the other hand, Norin 61 and RSD mutants showed no differences in the expressions of ABA inducible genes in seedlings. These results indicate that the mutations of RSD are specific in seed.
5. Comparison of gene expression by cDNA subtraction
Two clones (sbtc49 and sbtc63) were selected by the subtraction of cDNAs derived from embryos of Norin 61 and RSD32.
6. Proteomic analysis of two-dimensional protein-pattern
Quantitative and qualitative differences were detected in two-dimensional patterns of the protein extracted from embryos of Norin 61 and RSD32.
7. Screening of pre-harvest sprouting mutants
Twenty seven pre-harvest sprouting mutants (EPS: Enhanced Pre-harvest Sprouting) were screened by the germination in imbibed panicles from the population of mutagenized Norin 61 that showed resistance to pre-harvest sprouting.

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Study for genetic diversity of 'uzu'semi-dwarfbarley varieties in evolutionary, morphological and physiological aspects.

Grant number：16380008 2004 - 2006

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

TAKEDA Kazuyoshi, SAISHO Daisuke, RIKIISHI Kazuhide

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Grant amount：\15600000 （ Direct expense: \15600000 ）

In order to dissect the diversity of semi-dwarf barley variety 'uzu' in evolutionary, morphological and physiological aspects , we analyzed the genetic diversity of barley uzu varieties from three view points:(1) phylogeny of 'uzu' accessions,(2) genetic dissection of the 'uzu' accession specific morphological trait including the emergence of projection on coleoptile under high temperature condition, and (3) the physiological diversity of tissue culture traits including callus growth and shoot regeneration capacity. Results from these studies are:
(1) Phylogeographic analysis of five nuclear loci and morphological assessment of two traits in 282 barley accessions including 19 wild barley lines reveal that landraces distributed in South and East Asia are genetically distinct from those in 'Occidental' region. The six 'uzu' accessions investigated in this study possessed almost same haplotype among the five loci. These evidence strongly indicated that the origin of 'uzu' accessions was single mutation even.
(2) One of the 'uzu'-specific morphological traits, the emergence of projection on coleoptile under high temperature germination condition, is caused by ectopic cell division of coleoptiles apart from embryo genesis. Evaluation of this trait among 266 'uzu' accessions and segregation analysis of five F2 populations indicate that genetic factor (s) concerning to this character are genetically diverse within 'uzu' accessions. As the result of diallel analysis using 10 x 10 half diallel table, it is suggested that this trait is controlled by several additive genes.
(3) Effects of exogenous auxin and cytokinin on the emergence of projection on coleoptile were examined in three independent isogenic lines of barley with uzu gene. Although the emergence of projection was reduced in uzu lines under low temperature condition, both auxin and cytokinin enhanced the emergence of projection. These results indicate that auxin and cytokinin regulate the emergence of projection. These results indicate that auxin and cytokinin regulate the emergence of projection on coleoptile

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Genetic studies on regeneration and somaclonal variation in barley tissue culture.

Grant number：03454038 1991 - 1992

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (B)

YASUDA Shozo, RIKIISHI Kazuhide

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Grant amount：\3800000 （ Direct expense: \3800000 ）

This study was made using a total of 132 barley varieties which were collected from various regions in the world. The items of the experiments are as follows: (1) varietal variation and its geographical distribution in the rates of root and plantlet regenerations, (2) physiological analysis for callus formation and differentiation to the root and plantlet using mature and immature embryos, (3) diallel analysis for potentiality of callus formation, and plantlet regeneration, (4) effects of dry and wet conditions of culture medium, darkness and lightness for culture and content of hormonal substances in medium on regeneration of root and plantlets, (5) somaclonal variation in number of chromosomes and morphological characteristics, and (6) suspension culture of the pollen grains at the uninucleate stage. As the results, it was recognized that there were several factors affecting regenerational potentiality, and the gene or genes for root regeneration seemed to differ with those for plantlet regeneration. Secondary, it was suggested that the differences in the regeneration rate between mature embryos and immature embryos were affected by the hormonal contents in these embryos. Several environmental factors were considered as the third factor. Darkness and lightness, wet and dry media and high and low temperature etc. were tested in regeneration of root and plantlet, and darkness and dry medium raised regeneration rates in some varieties.

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C_3,C_4光合成系の遺伝様式に関する研究

Grant number：03760004 1991

日本学術振興会科学研究費助成事業奨励研究(A)

力石和英

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Grant amount：\900000 （ Direct expense: \900000 ）

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Plant Morphology （2024academic year） Third semester - 水1,水2
Plant Morphology （2023academic year） Third semester - 水1,水2
Plant Morphology （2022academic year） Third semester - 水1,水2
Plant Morphology （2021academic year） Third semester - 水1,水2
Plant Morphology （2020academic year） Third semester - 水1,水2