Updated on 2025/04/02

写真a

 
RIKIISHI Kazuhide
 
Organization
Scheduled update Assistant Professor
Position
Assistant Professor
External link

Degree

  • 農学修士 ( 東北大学 )

  • 農学博士 ( 東北大学 )

Research Interests

  • Plant Breeding

  • 植物育種学

Research Areas

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

Education

  • Tohoku University    

    - 1987

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  • Tohoku University   農学研究科   農学

    - 1987

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    Country: Japan

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  • Ibaraki University    

    - 1985

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  • Ibaraki University   農学部   農学

    - 1985

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    Country: Japan

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Research History

  • - Assistant Professor,Institute of Plant Science and Resources,Okayama University

    2004

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  • -

    2004

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Professional Memberships

Committee Memberships

  • 穂発芽研究会   幹事  

    2009   

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    Committee type:Academic society

    穂発芽研究会

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Papers

  • Transcriptomic analysis of developing seeds in a wheat (Triticum aestivum L.) mutant RSD32 with reduced seed dormancy Reviewed

    Rikiishi K, Sugimoto M, Maekawa M.

    Breeding Science   71   155 - 166   2021

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1270/jsbbs.20016.

  • Tamyb10-D1 restores red grain color and increases grain dormancy via suppressing expression of TaLTP2.128, non-specific lipid transfer protein in wheat Reviewed

    Eiko Himi, Shiho Kurihara‐Yonemoto, Fumitaka Abe, Hidekazu Takahashi, Keisuke Tanaka, Takakazu Matsuura, Masahiko Maekawa, Takuji Sasaki, Kazuhide Rikiishi

    Euphytica   220   16   2024.1

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10681-023-03265-3

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  • Rice Nudix Hydrolase OsNUDX2 Sanitizes Oxidized Nucleotides Reviewed

    Yuki Kondo, Kazuhide Rikiishi, Manabu Sugimoto

    Antioxidants   11 ( 9 )   1805 - 1805   2022.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Nudix hydrolase (NUDX) hydrolyzes 8-oxo-(d)GTP to reduce the levels of oxidized nucleotides in the cells. 8-oxo-(d)GTP produced by reactive oxygen species (ROS) is incorporated into DNA/RNA and mispaired with adenine, causing replicational and transcriptional errors. Here, we identified a rice OsNUDX2 gene, whose expression level was increased 15-fold under UV-C irradiation. The open reading frame of the OsNUDX2 gene, which encodes 776 amino acid residues, was cloned into Escherichia coli cells to produce the protein of 100 kDa. The recombinant protein hydrolyzed 8-oxo-dGTP, in addition to dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP), as did Arabidopsis AtNUDX1; whereas the amino acid sequence of OsNUDX2 had 18% identity with AtNUDX1. OsNUDX2 had 14% identity with barley HvNUDX12, which hydrolyzes 8-oxo-dGTP and diadenosine tetraphosphates. Suppression of the lacZ amber mutation caused by the incorporation of 8-oxo-GTP into mRNA was prevented to a significant degree when the OsNUDX2 gene was expressed in mutT-deficient E. coli cells. These results suggest that the different substrate specificity and identity among plant 8-oxo-dGTP-hydrolyzing NUDXs and OsNUDX2 reduces UV stress by sanitizing the oxidized nucleotides.

    DOI: 10.3390/antiox11091805

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  • The Presence of the Hairy-Root-Disease-Inducing (Ri) Plasmid in Wheat Endophytic Rhizobia Explains a Pathogen Reservoir Function of Healthy Resistant Plants Reviewed International journal

    Byoungwoo Kang, Taichi Maeshige, Aya Okamoto, Yui Kataoka, Shinji Yamamoto, Kazuhide Rikiishi, Akio Tani, Hiroyuki Sawada, Katsunori Suzuki

    Applied and Environmental Microbiology   86 ( 17 )   2020.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    Crown gall and hairy root diseases exhibit very wide host-plant ranges that cover gymnosperm and dicot plants. The
    <named-content content-type="genus-species">Rhizobium radiobacter</named-content>
    species complex harbors causative agents of the two diseases. Recently, endophyte isolates from many plant species have been assigned to this species complex. We isolated seven endophyte strains belonging to the species complex from wheat plants and revealed their genomovar affiliations and plasmid profile. The significance of this study is the finding of the genomovar correlation between the endophytes and the known pathogens, the presence of a virulence ability in two of the seven endophyte strains, and the high ratio of the pathogenic strains in the endophyte strains. This study therefore provides convincing evidence that could unravel the mechanism that maintains pathogenic agents of this species and sporadically delivers them to susceptible plants.

    DOI: 10.1128/aem.00671-20

    PubMed

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  • Establishment of nDart1-tagged lines of Koshihikari, an elite variety of rice in Japan. Reviewed

    Hideki Nishimura, Eiko Himi, Kazuhide Rikiishi, Kazuo Tsugane, Masahiko Maekawa

    Breeding science   69 ( 4 )   696 - 701   2019.12

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    Language:English  

    To utilize a transposon-tagged mutant as a breeding material in rice, an endogenous DNA transposon, nDart1-0, was introduced into Koshihikari by successive backcrossing together with aDart1-27, an active autonomous element. The founder line for nDart1-tagged lines of Koshihikari carried nDart1-0 on chromosome 9 and transposed nDart1-12s on chromosomes 1 and 8 and nDart1-3 on chromosome 11. In nDart1-tagged lines, there were the most abnormal phenotypic mutants and many aberrant chlorophyll mutants at seedling stage. At mature stage, many semi-sterile mutants were observed. Dwarf, reduced culm number and lesion mimic mutants were also found. In total, 43.2% of the lines segregated some phenotypic mutants. Thus, the nDart1-tagged lines of Koshihikari are expected to be potentially useful for screening stress-tolerant mutants under abiotic or biotic stress conditions.

    DOI: 10.1270/jsbbs.19049

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MISC

  • A novel role for lipid-transfer protein in the control of wheat seed dormancy

    氷見英子, 栗原志保, 安倍史高, 高橋秀和, 田中啓介, 松浦恭和, 前川雅彦, 力石和英

    育種学研究   24   2022

  • コムギ種子休眠性遺伝子 ~突然変異系統の解析から見えてきたこと~

    力石和英

    穂発芽被害ゼロをめざして 穂発芽研究会20周年記念誌   50 - 57   2019

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    Authorship:Lead author, Corresponding author   Language:Japanese  

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  • Genetic analysis of a large grain mutant found in transposon-tagged lines in rice

    Chiou w, K. Rikiishi, E. Himi, H. Nishimura, K. Tsugane, M. Maekawa

    育種学研究   19 ( 別1 )   55 - 55   2017

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  • オオムギ未熟胚培養系の植物体再分化における光制御機構のマイクロアレイ解析

    力石和英, 西村秀希, 前川雅彦

    育種学研究   18 ( 別2 )   211 - 211   2016

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  • オオムギ未熟胚由来カルスからの植物体再分化における光制御は内生ABA含量の増加により引き起こされる

    力石和英, 松浦恭和, 池田陽子, 前川雅彦

    育種学研究   17 ( 別2 )   218 - 218   2015

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Presentations

  • MutMap法を用いたコムギの種子休眠性低下突然変異rsd32の同定

    力石和英, 土屋江利, 杉本学

    穂発芽研究会  2024.12.10 

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    Event date: 2024.12.10

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • MutMap法によるコムギの種子休眠性低下突然変異の原因遺伝子rsd32の同定

    力石和英, 土屋江利, 杉本学

    種子生理生化学研究会年会  2024.11.22 

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    Event date: 2024.11.21 - 2024.11.22

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • MutMap法を用いたコムギの種子休眠性低下突然変異rsd32の同定

    力石和英, 土屋江利, 杉本学

    日本育種学会  2024.9.20 

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    Event date: 2024.9.19 - 2024.9.20

    Language:Japanese   Presentation type:Poster presentation  

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  • 酸化ヌクレオチド除去に関与するトマトNudix hydrolaseの探索

    横山聡太郎, 力石和英, 杉本 学

    日本農芸化学会  2024.3.24 

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    Event date: 2024.3.24 - 2024.3.27

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • コムギ内生菌アグロバクテリウムのサイトカイニン合成と合成遺伝子クラスター

    鈴木克周, 清川一矢, 山篠貴史, 力石和英

    第46回日本分子生物学会年会  2023.12.6 

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    Event date: 2023.12.6 - 2023.12.8

    Language:Japanese  

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Research Projects

  • コムギ種子休眠性を誘導する新規の種子特異的制御因子の機能解明とその育種的利用

    Grant number:22K05573  2022.04 - 2025.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    力石 和英

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

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  • Cereal plant transformation using endophytic agrobacteria

    Grant number:21H02167  2021.04 - 2025.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    鈴木 克周, 力石 和英

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    Grant amount:\17290000 ( Direct expense: \13300000 、 Indirect expense:\3990000 )

    主要穀物植物は単子葉植物であり、病原性アグロバクテリア(Agrobacterium tumefaciens)による感染を受けず、ムギ類に代表されるようにアグロバクテリアを用いる形質転換が難しい。本研究は植物の形質転換問題を改善することを目標としている。先ず、申請者らがコムギから単離したアグロバクテリア内生菌株を植物形質転換に汎用的に利用できるドナー株に改変加工を行う。次いで、胚性カルスの形質転換へ適用する評価試験を行ってどのような特性を持つか明らかにすることで、世界で初めて有用株化した内生細菌株を公開し、広く形質転換に活用できるように整備することが本研究の目的である。
    既に申請者らがコムギから単離したアグロバクテリア(A. tumefaciens)内生菌株には、Riプラスミドを持つG7グループ菌株とTiプラスミド/Riプラスミドどちらをも持たないG1グループ菌株の計2系統がある。2021年度の実施計画は主に、それぞれの内生菌株でT-DNA輸送能力を持つが病気を生じさせない(アームレス化)ドナー菌株を作成することと、来年度本格実施する植物形質転換の準備を行うという内容である。
    G1グループ内生菌株へアームレスRiプラスミド導入した菌株を作成した。G7グループ内生菌株については、Riプラスミド除去を行ったうえでアームレスRiプラスミド導入した菌株を作成した。一方、既存のTiプラスミド導入体を得ることができなかった。G7グループ内生菌株RN3の塩基配列決定によって、野生型RiプラスミドpRiRN3は長さ234,326 bpであり、1つのT-DNAを持っていることが判った。この配列情報に基づいて、T-DNA部分のみを除去するための加工用プラスミドを設計した。

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  • Analysis of wheat mutants in reduced seed dormancy

    Grant number:16580005  2004 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    RIKIISHI Kazuhide

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    Grant amount:\3400000 ( Direct expense: \3400000 )

    In this project, wheat mutants in reduced seed dormancy were analyzed for understanding the regulations of seed dormancy and pre-harvest sprouting in wheat.
    1. ABA sensitivity in RSD (Reduced Seed Dormancy) mutants
    RSD mutants showed lower sensitivity to exogenous ABA and the levels of sensitivity were different among mutant lines. Seed developmental stages affected ABA sensitivity of Norin 61 and RSD mutants and the different responses had shown among mutants.
    2. Genetic analysis
    Reduced seed dormancy of RSD14-1 and RSD12 were dominant mutations and those of RSD16-1 and RSD32 were recessive mutations. Segregation ratio for reduced seed dormancy in F2 population derived from the cross between Norin 61 and RSD32, suggests that mutant phenotype of RSD32 is a single recessive mutation.
    3. Analysis of the regulation for a-amylase expression
    RSD mutants showed the higher expression of a-amylase in embryos than Norin 61. Expression levels of genes that were negative regulator of a-amylase and showed seed specific manner were determined. TaABF were expressed lower levels in three RSD mutants. Two RSD mutants also showed lower expression of TaAFP.
    4. Regulation of ABA inducible genes in RSD mutants
    Expression of Em genes that were induced by ABA and specifically expressed in seeds were lower level in RSD mutants, suggesting that ABA signals diminished in seed of RSD mutants. On the other hand, Norin 61 and RSD mutants showed no differences in the expressions of ABA inducible genes in seedlings. These results indicate that the mutations of RSD are specific in seed.
    5. Comparison of gene expression by cDNA subtraction
    Two clones (sbtc49 and sbtc63) were selected by the subtraction of cDNAs derived from embryos of Norin 61 and RSD32.
    6. Proteomic analysis of two-dimensional protein-pattern
    Quantitative and qualitative differences were detected in two-dimensional patterns of the protein extracted from embryos of Norin 61 and RSD32.
    7. Screening of pre-harvest sprouting mutants
    Twenty seven pre-harvest sprouting mutants (EPS: Enhanced Pre-harvest Sprouting) were screened by the germination in imbibed panicles from the population of mutagenized Norin 61 that showed resistance to pre-harvest sprouting.

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  • Study for genetic diversity of 'uzu'semi-dwarfbarley varieties in evolutionary, morphological and physiological aspects.

    Grant number:16380008  2004 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    TAKEDA Kazuyoshi, SAISHO Daisuke, RIKIISHI Kazuhide

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    Grant amount:\15600000 ( Direct expense: \15600000 )

    In order to dissect the diversity of semi-dwarf barley variety 'uzu' in evolutionary, morphological and physiological aspects , we analyzed the genetic diversity of barley uzu varieties from three view points:(1) phylogeny of 'uzu' accessions,(2) genetic dissection of the 'uzu' accession specific morphological trait including the emergence of projection on coleoptile under high temperature condition, and (3) the physiological diversity of tissue culture traits including callus growth and shoot regeneration capacity. Results from these studies are:
    (1) Phylogeographic analysis of five nuclear loci and morphological assessment of two traits in 282 barley accessions including 19 wild barley lines reveal that landraces distributed in South and East Asia are genetically distinct from those in 'Occidental' region. The six 'uzu' accessions investigated in this study possessed almost same haplotype among the five loci. These evidence strongly indicated that the origin of 'uzu' accessions was single mutation even.
    (2) One of the 'uzu'-specific morphological traits, the emergence of projection on coleoptile under high temperature germination condition, is caused by ectopic cell division of coleoptiles apart from embryo genesis. Evaluation of this trait among 266 'uzu' accessions and segregation analysis of five F2 populations indicate that genetic factor (s) concerning to this character are genetically diverse within 'uzu' accessions. As the result of diallel analysis using 10 x 10 half diallel table, it is suggested that this trait is controlled by several additive genes.
    (3) Effects of exogenous auxin and cytokinin on the emergence of projection on coleoptile were examined in three independent isogenic lines of barley with uzu gene. Although the emergence of projection was reduced in uzu lines under low temperature condition, both auxin and cytokinin enhanced the emergence of projection. These results indicate that auxin and cytokinin regulate the emergence of projection. These results indicate that auxin and cytokinin regulate the emergence of projection on coleoptile

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  • Genetic studies on regeneration and somaclonal variation in barley tissue culture.

    Grant number:03454038  1991 - 1992

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for General Scientific Research (B)

    YASUDA Shozo, RIKIISHI Kazuhide

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    Grant amount:\3800000 ( Direct expense: \3800000 )

    This study was made using a total of 132 barley varieties which were collected from various regions in the world. The items of the experiments are as follows: (1) varietal variation and its geographical distribution in the rates of root and plantlet regenerations, (2) physiological analysis for callus formation and differentiation to the root and plantlet using mature and immature embryos, (3) diallel analysis for potentiality of callus formation, and plantlet regeneration, (4) effects of dry and wet conditions of culture medium, darkness and lightness for culture and content of hormonal substances in medium on regeneration of root and plantlets, (5) somaclonal variation in number of chromosomes and morphological characteristics, and (6) suspension culture of the pollen grains at the uninucleate stage. As the results, it was recognized that there were several factors affecting regenerational potentiality, and the gene or genes for root regeneration seemed to differ with those for plantlet regeneration. Secondary, it was suggested that the differences in the regeneration rate between mature embryos and immature embryos were affected by the hormonal contents in these embryos. Several environmental factors were considered as the third factor. Darkness and lightness, wet and dry media and high and low temperature etc. were tested in regeneration of root and plantlet, and darkness and dry medium raised regeneration rates in some varieties.

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Class subject in charge

  • Plant Morphology (2024academic year) Third semester  - 水1,水2

  • Plant Morphology (2023academic year) Third semester  - 水1,水2

  • Plant Morphology (2022academic year) Third semester  - 水1,水2

  • Plant Morphology (2021academic year) Third semester  - 水1,水2

  • Plant Morphology (2020academic year) Third semester  - 水1,水2