Faculty Profiles - MORI Izumi.C

Papers - MORI Izumi.C

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Sulfur dioxide-induced guard cell death and stomatal closure are attenuated in nitrate/proton antiporter AtCLCa mutants

Lia Ooi, Takakazu Matsuura, Izumi C Mori

Plant And Cell Physiology 2025.6

Amino Acid Substitutions in Loop C of Arabidopsis PIP2 Aquaporins Alters the Permeability of CO2

Shaila Shermin Tania, Shigeko Utsugi, Yoshiyuki Tsuchiya, Shizuka Sasano, Maki Katsuhara, Izumi C. Mori

Plant, Cell & Environment 2025.6

Physiological and Biochemical Traits of Dormancy Release and Growth Resumption in Japanese Cedar in the Warm-Temperate Zone

Shoma Hiejima, Hiroto Seino, Rico Hachisuka, Yuka Watanabe, Takakazu Matsuura, Izumi C. Mori, Shin Ugawa

Forest Science 2025.6

TGG1 and TGG2 mutations impair allyl isothiocyanate-mediated stomatal closure in Arabidopsis thaliana. International journal

Kadri Oumaima, Mohammad Shakhawat Hossain, Wenxiu Ye, Eiji Okuma, Mohammad Issak, Mohammad Mahbub Islam, Misugi Uraji, Yoshimasa Nakamura, Izumi C Mori, Shintaro Munemasa, Yoshiyuki Murata

Protoplasma 2025.2

Language：English Publishing type：Research paper (scientific journal)

Myrosinase, referred to as thioglucoside glucohydrolase (TGG), plays a crucial role in plant physiology through catalyzing the hydrolysis of glucosinolates into bioactive isothiocyanates. In Arabidopsis thaliana, the myrosinases TGG1 and TGG2 are essential for abscisic acid- and methyl jasmonate-induced stomata closure. Allyl isothiocyanate (AITC), one of myrosinase products, triggers stomatal closure in A. thaliana. We investigated stomatal responses to AITC to clarify the role of TGG1 and TGG2 in Arabidopsis guard-cell signaling. Allyl isothiocyanate at 50 μM and 100 μM induced stomatal closure in the tgg1 and tgg2 single mutants but not in the tgg1 tgg2 double mutant. Furthermore, AITC at 50 μM induced the production of reactive oxygen species and nitric oxide, cytosolic alkalization, and oscillations in cytosolic free calcium concentration in guard cells of both wild-type and mutant plants. These findings suggest that TGG1 and TGG2 are involved in AITC signaling pathway through interaction with signal component(s) downstream of these signaling events, which is not accompanied by hydrolysis of glucosinolates because of the difference in subcellular localization between enzymes (myrosinases) and substrates (glucosinolates).

DOI： 10.1007/s00709-025-02039-z

PubMed

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Plant growth‐promoting abilities of Methylobacterium sp. 2A involve auxin‐mediated regulation of the root architecture Reviewed

Cecilia E. M. Grossi, Akio Tani, Izumi C. Mori, Takakazu Matsuura, Rita M. Ulloa

Plant, Cell & Environment 2024.8

Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Abstract

Methylobacterium sp. 2A, a plant growth‐promoting rhizobacteria (PGPR) able to produce indole‐3‐acetic acid (IAA), significantly promoted the growth of Arabidopsis thaliana plants in vitro. We aimed to understand the determinants of Methylobacterium sp. 2A–A. thaliana interaction, the factors underlying plant growth‐promotion and the host range. Methylobacterium sp. 2A displayed chemotaxis to methanol and formaldehyde and was able to utilise 1‐aminocyclopropane carboxylate as a nitrogen source. Confocal microscopy confirmed that fluorescent protein‐labelled Methylobacterium sp. 2A colonises the apoplast of A. thaliana primary root cells and its inoculation increased jasmonic and salicylic acid in A. thaliana, while IAA levels remained constant. However, inoculation increased DR5 promoter activity in root tips of A. thaliana and tomato plants. Inoculation of this PGPR partially restored the agravitropic response in yucQ mutants and lateral root density was enhanced in iaa19, arf7, and arf19 mutant seedlings. Furthermore, Methylobacterium sp. 2A volatile organic compounds (VOCs) had a dose‐dependent effect on the growth of A. thaliana. This PGPR is also able to interact with monocots eliciting positive responses upon inoculation. Methylobacterium sp. 2A plant growth‐promoting effects can be achieved through the regulation of plant hormone levels and the emission of VOCs that act either locally or at a distance.

DOI： 10.1111/pce.15116

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Cytosolic acidification and oxidation are the toxic mechanisms of SO2 in Arabidopsis guard cells Reviewed

Mahdi Mozhgani, Lia Ooi, Christelle Espagne, Sophie Filleur, Izumi C Mori

Bioscience, Biotechnology, and Biochemistry 2024.7

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

ABSTRACT

SO2/H2SO3 can damage plants. However, its toxic mechanism has still been controversial. Two models have been proposed, cytosolic acidification model and cellular oxidation model. Here, we assessed the toxic mechanism of H2SO3 in three cell types of Arabidopsis thaliana, mesophyll cells, guard cells (GCs), and petal cells. The sensitivity of GCs of Chloride channel a (CLCa)-knockout mutants to H2SO3 was significantly lower than those of wildtype plants. Expression of other CLC genes in mesophyll cells and petal cells were different from GCs. Treatment with antioxidant, disodium 4,5-dihydroxy-1,3-benzenedisulfonate (tiron), increased the median lethal concentration (LC50) of H2SO3 in GCs indicating the involvement of cellular oxidation, while the effect was negligible in mesophyll cells and petal cells. These results indicate that there are two toxic mechanisms of SO2 to Arabidopsis cells: cytosolic acidification and cellular oxidation, and the toxic mechanism may vary among cell types.

DOI： 10.1093/bbb/zbae092

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MAP4K1 and MAP4K2 regulate ABA-induced and Ca2+-mediated stomatal closure in Arabidopsis

Kota Yamashita, Sotaro Katagiri, Hinano Takase, Yangdan Li, Airi Otoguro, Yoshiaki Kamiyama, Shota Yamauchi, Atsushi Takemiya, Izumi C. Mori, Taishi Umezawa

2024.7

Florigen-producing cells express FPF1-LIKE PROTEIN 1 that accelerates flowering and stem growth in long days with sunlight red/far-red ratio in Arabidopsis

Hiroshi Takagi, Nayoung Lee, Andrew K. Hempton, Savita Purushwani, Michitaka Notaguchi, Kota Yamauchi, Kazumasa Shirai, Yaichi Kawakatsu, Susumu Uehara, William G. Albers, Benjamin L. R. Downing, Shogo Ito, Takamasa Suzuki, Takakazu Matsuura, Izumi C. Mori, Nobutaka Mitsuda, Daisuke Kurihara, Tomonao Matsushita, Young Hun Song, Yoshikatsu Sato, Mika Nomoto, Yasuomi Tada, Kousuke Hanada, Josh Cuperus, Christine Queitsch, Takato Imaizumi

2024.4

Publisher：Cold Spring Harbor Laboratory

Seasonal changes in spring induce flowering by expressing the florigen, FLOWERING LOCUS T (FT), in Arabidopsis. FT is expressed in unique phloem companion cells with unknown characteristics. The question of which genes are co-expressed with FT and whether they have roles in flowering remains elusive. Through tissue-specific translatome analysis, we discovered that under long-day conditions with the natural sunlight red/far-red ratio, the FT-producing cells express a gene encoding FPF1-LIKE PROTEIN 1 (FLP1). The master FT regulator, CONSTANS (CO), controls FLP1 expression, suggesting FLP1's involvement in the photoperiod pathway. FLP1 promotes early flowering independently of FT, is active in the shoot apical meristem, and induces the expression of SEPALLATA 3 (SEP3), a key E-class homeotic gene. Unlike FT, FLP1 facilitates inflorescence stem elongation. Our cumulative evidence indicates that FLP1 may act as a mobile signal. Thus, FLP1 orchestrates floral initiation together with FT and promotes inflorescence stem elongation during reproductive transitions.

DOI： 10.1101/2024.04.26.591289

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Possible roles of immunity-related response in modulating chlorosis induced by the silencing of chloroplast HSP90C in tobacco models

Okon Odiong Unung, Houssam Eddine Said Bensedira, Takakazu Matsuura, Izumi C. Mori, Yuta Shimomura, Takashi Yaeno, Hidetaka Kaya, Kappei Kobayashi

Journal of General Plant Pathology 2024

MHP1 and MHL generate odd-chain fatty acids from 2-hydroxy fatty acids in sphingolipids and are related to immunity in Arabidopsis thaliana

Marina Ushio, Toshiki Ishikawa, Takakazu Matsuura, Izumi C. Mori, Maki Kawai-Yamada, Yoichiro Fukao, Minoru Nagano

Plant Science 336 111840 - 111840 2023.11

The effect of exogenous dihydroxyacetone and methylglyoxal on growth, anthocyanin accumulation, and the glyoxalase system in Arabidopsis. International journal

Maoxiang Zhao, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Izumi C Mori, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 2023.8

Language：English Publishing type：Research paper (scientific journal)

Dihydroxyacetone (DHA) occurs in wide-ranging organisms including plants and can undergo spontaneous conversion to methylglyoxal (MG). While the toxicity of MG to plants is well-known, the toxicity of DHA to plants remains to be elucidated. We investigated the effects of DHA and MG on Arabidopsis. Exogenous DHA at up to 10 m m did not affect the radicle emergence, the expansion of green cotyledons, the seedling growth, or the activity of glyoxalase II while DHA at 10 m m inhibited the root elongation and increased the activity of glyoxalase I. Exogenous MG at 1.0 m m inhibited these physiological responses and increased both activities. DHA at 10 m m increased the MG content in the roots. These results indicate that DHA is not so toxic as MG in Arabidopsis seeds and seedlings and suggest that the toxic effect of DHA at high concentrations is attributed to MG accumulation by the conversion to MG.

DOI： 10.1093/bbb/zbad109

PubMed

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Zinc deficiency-induced defensin-like proteins are involved in the inhibition of root growth in Arabidopsis

Sachie Kimura, Aleksia Vaattovaara, Tomoya Ohshita, Kotomi Yokoyama, Kota Yoshida, Agnes Hui, Hidetaka Kaya, Ai Ozawa, Mami Kobayashi, Izumi C. Mori, Yoshiyuki Ogata, Yoko Ishino, Shigeo S. Sugano, Minoru Nagano, Yoichiro Fukao

PLANT JOURNAL 2023.6

Application of trehalose mitigates short-styled flowers in Solanaceous crops Reviewed

Izumi C. Mori, Takakazu Matsuura, Masahiro Otao, Lia Ooi, Yasuyo Nishimura, Takashi Hirayama

Journal of Agricultural and Food Chemistry 71 ( 14 ) 5476 - 5482 2023.4

Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana

Mamoru Matsumura, Mika Nomoto, Tomotaka Itaya, Yuri Aratani, Mizuki Iwamoto, Takakazu Matsuura, Yuki Hayashi, Tsuyoshi Mori, Michael J. Skelly, Yoshiharu Y. Yamamoto, Toshinori Kinoshita, Izumi C. Mori, Takamasa Suzuki, Shigeyuki Betsuyaku, Steven H. Spoel, Masatsugu Toyota, Yasuomi Tada

Nature Communications 13 ( 1 ) 2022.12

Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

<title>Abstract</title>Perception of pathogen-derived ligands by corresponding host receptors is a pivotal strategy in eukaryotic innate immunity. In plants, this is complemented by circadian anticipation of infection timing, promoting basal resistance even in the absence of pathogen threat. Here, we report that trichomes, hair-like structures on the epidermis, directly sense external mechanical forces, including raindrops, to anticipate pathogen infections in <italic>Arabidopsis thaliana</italic>. Exposure of leaf surfaces to mechanical stimuli initiates the concentric propagation of intercellular calcium waves away from trichomes to induce defence-related genes. Propagating calcium waves enable effective immunity against pathogenic microbes through the CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 3 (CAMTA3) and mitogen-activated protein kinases. We propose an early layer of plant immunity in which trichomes function as mechanosensory cells that detect potential risks.

DOI： 10.1038/s41467-022-28813-8

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Other Link： https://www.nature.com/articles/s41467-022-28813-8

Proteasome-associated ubiquitin ligase relays target plant hormone-specific transcriptional activators Reviewed

Zhishuo Wang, Beatriz Orosa-Puente, Mika Nomoto, Heather Grey, Thomas Potuschak, Takakazu Matsuura, Izumi C. Mori, Yasuomi Tada, Pascal Genschik, Steven H. Spoel

Science Advances 8 ( 42 ) 2022.10

Language：English Publishing type：Research paper (scientific journal) Publisher：American Association for the Advancement of Science (AAAS)

The ubiquitin-proteasome system is vital to hormone-mediated developmental and stress responses in plants. Ubiquitin ligases target hormone-specific transcriptional activators (TAs) for degradation, but how TAs are processed by proteasomes remains unknown. We report that in Arabidopsis , the salicylic acid– and ethylene-responsive TAs, NPR1 and EIN3, are relayed from pathway-specific ubiquitin ligases to proteasome-associated HECT-type UPL3/4 ligases. Activity and stability of NPR1 were regulated by sequential action of three ubiquitin ligases, including UPL3/4, while proteasome processing of EIN3 required physical handover between ethylene-responsive SCF ^EBF2 and UPL3/4 ligases. Consequently, UPL3/4 controlled extensive hormone-induced developmental and stress-responsive transcriptional programs. Thus, our findings identify unknown ubiquitin ligase relays that terminate with proteasome-associated HECT-type ligases, which may be a universal mechanism for processive degradation of proteasome-targeted TAs and other substrates.

DOI： 10.1126/sciadv.abn4466

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Functional roles of ALMT‐type anion channels in malate‐induced stomatal closure in tomato and Arabidopsis

Takayuki Sasaki, Michiyo Ariyoshi, Yoko Yamamoto, Izumi C. Mori

Plant, Cell and Environment 2022.6

Cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize Reviewed

Lae‐Hyeon Cho, Jinmi Yoon, Win Tun, Gibeom Baek, Xin Peng, Woo‐Jong Hong, Izumi C. Mori, Yuko Hojo, Takakazu Matsuura, Sung‐Ryul Kim, Sun‐Tae Kim, Soon‐Wook Kwon, Ki‐Hong Jung, Jong‐Seong Jeon, Gynheung An

The Plant Journal 110 ( 6 ) 1619 - 1635 2022.4

Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

SUMMARY

Increasing the vegetative growth period of crops can increase biomass and grain yield. In rice (Oryza sativa), the concentration of trans ‐zeatin, an active cytokinin, was high in the leaves during vegetative growth and decreased rapidly upon induction of florigen expression, suggesting that this hormone is involved in the regulation of the vegetative phase. To elucidate whether exogenous cytokinin application influences the length of the vegetative phase, we applied 6‐benzylaminopurine (BAP) to rice plants at various developmental stages. Our treatment delayed flowering time by 8–9 days when compared with mock‐treated rice plants, but only at the transition stage when the flowering signals were produced. Our observations also showed that flowering in the paddy field is delayed by thidiazuron, a stable chemical that mimics the effects of cytokinin. The transcript levels of florigen genes Heading date 3a (Hd3a) and Rice Flowering locus T1 (RFT1) were significantly reduced by the treatment, but the expression of Early heading date 1 (Ehd1), a gene found directly upstream of the florigen genes, was not altered. In maize (Zea mays), similarly, BAP treatment increased the vegetative phage by inhibiting the expression of ZCN8, an ortholog of Hd3a. We showed that cytokinin treatment induced the expression of two type‐A response regulators (OsRR1 and OsRR2) which interacted with Ehd1, a type‐B response regulator. We also observed that cytokinin did not affect flowering time in ehd1 knockout mutants. Our study indicates that cytokinin application increases the duration of the vegetative phase by delaying the expression of florigen genes in rice and maize by inhibiting Ehd1.

DOI： 10.1111/tpj.15760

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.15760

Abscisic acid is required for exodermal suberization to form a barrier to radial oxygen loss in the adventitious roots of rice (Oryza sativa). International journal

Katsuhiro Shiono, Marina Yoshikawa, Tino Kreszies, Sumiyo Yamada, Yuko Hojo, Takakazu Matsuura, Izumi C Mori, Lukas Schreiber, Toshihito Yoshioka

The New phytologist 233 ( 2 ) 655 - 669 2022.1

Language：English Publishing type：Research paper (scientific journal)

To acclimate to waterlogged conditions, wetland plants form a barrier to radial oxygen loss (ROL) that can enhance oxygen transport to the root apex. We hypothesized that one or more hormones are involved in the induction of the barrier and searched for such hormones in rice. We previously identified 98 genes that were tissue-specifically upregulated during ROL barrier formation in rice. The RiceXPro database showed that most of these genes were highly enhanced by exogenous abscisic acid (ABA). We then examined the effect of ABA on ROL barrier formation by using an ABA biosynthesis inhibitor (fluridone, FLU), by applying exogenous ABA and by examining a mutant with a defective ABA biosynthesis gene (osaba1). FLU suppressed barrier formation in a stagnant solution that mimics waterlogged soil. Under aerobic conditions, rice does not naturally form a barrier, but 24 h of ABA treatment induced barrier formation. osaba1 did not form a barrier under stagnant conditions, but the application of ABA rescued the barrier. In parallel with ROL barrier formation, suberin lamellae formed in the exodermis. These findings strongly suggest that ABA is an inducer of suberin lamellae formation in the exodermis, resulting in an ROL barrier formation in rice.

DOI： 10.1111/nph.17751

PubMed

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Cadmium uptake via apoplastic bypass flow in Oryza sativa Reviewed

Izumi C. Mori, Carlos Raul Arias-Barreiro, Lia Ooi, Nam-Hee Lee, Muhammad Abdus Sobahan, Yoshimasa Nakamura, Yoshihiko Hirai, Yoshiyuki Murata

Journal of Plant Research 134 ( 5 ) 1139 - 1148 2021.9

Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media {LLC}

It is known that rice roots take up cadmium (Cd) via the symplastic route mediated by membrane-bound mineral transporters. Here we provide evidence that apoplastic bypass flow is another Cd uptake route in rice. High concentrations of Cd rendered apoplastic bypass flow rate increased in rice seedlings. These concentrations of Cd compromised membrane integrity in the root meristem and transition zone. Polyethleneglycol and proline inhibited the Cd-induced apoplastic bypass flow and Cd transfer to the shoots. Loss-of-function mutant of the Cd uptake transporter, nramp5, showed Cd transport to the shoot comparable to the wild type. At a low Cd concentration, increased apoplastic bypass flow rate by NaCl stress resulted in an elevation of Cd transport to shoots both in the wildtype and nramp5. These observations indicate that apoplastic bypass flow in roots carries Cd transport leading to xylem loading of Cd in addition to the symplastic pathway mediated by mineral transporters under stressed conditions.

DOI： 10.1007/s10265-021-01319-y

PubMed

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Salicylic Acid Acts Antagonistically to Plastid Retrograde Signaling by Promoting the Accumulation of Photosynthesis-associated Proteins in Arabidopsis Reviewed

Yoshihiro Hirosawa, Akari Tada, Takakazu Matsuura, Izumi C Mori, Yoshitoshi Ogura, Tetsuya Hayashi, Susumu Uehara, Yasuko Ito-Inaba, Takehito Inaba

Plant and Cell Physiology 2021.8

Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press ({OUP})

Plastids are involved in phytohormone metabolism as well as photosynthesis. However, the mechanism by which plastid retrograde signals and phytohormones cooperatively regulate plastid biogenesis remains elusive. Here, we investigated the effects of an inhibitor and a mutation that generate biogenic plastid signals on phytohormones, and vice versa. Inhibition of plastid biogenesis by norflurazon (NF)-treatment and the plastid protein import2 (ppi2) mutation caused a decrease in salicylic acid (SA) and jasmonic acid (JA). This effect can be attributed in part to the altered expression of genes involved in the biosynthesis and metabolism of SA and JA. However, SA-dependent induction of the PATHOGENESIS-RELATED1 gene was virtually unaffected in NF-treated plants and the ppi2 mutant. Instead, the level of chlorophyll in these plants was partially restored by exogenous application of SA. Consistent with this observation, the levels of some photosynthesis-associated proteins increased in the ppi2 and NF-treated plants in response to SA treatment. This regulation in true leaves seems to occur at post-transcriptional level, since SA treatment did not induce the expression of photosynthesis associated genes. In salicylic acid induction deficient 2 and lesions simulating disease resistance 1 mutants, endogenous SA regulates the accumulation of photosynthesis-associated proteins through transcriptional and post-transcriptional mechanisms. These data indicate that SA acts antagonistically to the inhibition of plastid biogenesis by promoting the accumulation of photosynthesis-associated proteins in Arabidopsis, suggesting a possible link between SA and biogenic plastid signaling.

DOI： 10.1093/pcp/pcab128

PubMed

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Papers - MORI Izumi.C