Language：English   Publishing type：Research paper (scientific journal)  

Myrosinase, referred to as thioglucoside glucohydrolase (TGG), plays a crucial role in plant physiology through catalyzing the hydrolysis of glucosinolates into bioactive isothiocyanates. In Arabidopsis thaliana, the myrosinases TGG1 and TGG2 are essential for abscisic acid- and methyl jasmonate-induced stomata closure. Allyl isothiocyanate (AITC), one of myrosinase products, triggers stomatal closure in A. thaliana. We investigated stomatal responses to AITC to clarify the role of TGG1 and TGG2 in Arabidopsis guard-cell signaling. Allyl isothiocyanate at 50 μM and 100 μM induced stomatal closure in the tgg1 and tgg2 single mutants but not in the tgg1 tgg2 double mutant. Furthermore, AITC at 50 μM induced the production of reactive oxygen species and nitric oxide, cytosolic alkalization, and oscillations in cytosolic free calcium concentration in guard cells of both wild-type and mutant plants. These findings suggest that TGG1 and TGG2 are involved in AITC signaling pathway through interaction with signal component(s) downstream of these signaling events, which is not accompanied by hydrolysis of glucosinolates because of the difference in subcellular localization between enzymes (myrosinases) and substrates (glucosinolates).

DOI： 10.1007/s00709-025-02039-z

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

Abstract

Methylobacterium sp. 2A, a plant growth‐promoting rhizobacteria (PGPR) able to produce indole‐3‐acetic acid (IAA), significantly promoted the growth of Arabidopsis thaliana plants in vitro. We aimed to understand the determinants of Methylobacterium sp. 2A–A. thaliana interaction, the factors underlying plant growth‐promotion and the host range. Methylobacterium sp. 2A displayed chemotaxis to methanol and formaldehyde and was able to utilise 1‐aminocyclopropane carboxylate as a nitrogen source. Confocal microscopy confirmed that fluorescent protein‐labelled Methylobacterium sp. 2A colonises the apoplast of A. thaliana primary root cells and its inoculation increased jasmonic and salicylic acid in A. thaliana, while IAA levels remained constant. However, inoculation increased DR5 promoter activity in root tips of A. thaliana and tomato plants. Inoculation of this PGPR partially restored the agravitropic response in yucQ mutants and lateral root density was enhanced in iaa19, arf7, and arf19 mutant seedlings. Furthermore, Methylobacterium sp. 2A volatile organic compounds (VOCs) had a dose‐dependent effect on the growth of A. thaliana. This PGPR is also able to interact with monocots eliciting positive responses upon inoculation. Methylobacterium sp. 2A plant growth‐promoting effects can be achieved through the regulation of plant hormone levels and the emission of VOCs that act either locally or at a distance.

DOI： 10.1111/pce.15116

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

ABSTRACT

SO2/H2SO3 can damage plants. However, its toxic mechanism has still been controversial. Two models have been proposed, cytosolic acidification model and cellular oxidation model. Here, we assessed the toxic mechanism of H2SO3 in three cell types of Arabidopsis thaliana, mesophyll cells, guard cells (GCs), and petal cells. The sensitivity of GCs of Chloride channel a (CLCa)-knockout mutants to H2SO3 was significantly lower than those of wildtype plants. Expression of other CLC genes in mesophyll cells and petal cells were different from GCs. Treatment with antioxidant, disodium 4,5-dihydroxy-1,3-benzenedisulfonate (tiron), increased the median lethal concentration (LC50) of H2SO3 in GCs indicating the involvement of cellular oxidation, while the effect was negligible in mesophyll cells and petal cells. These results indicate that there are two toxic mechanisms of SO2 to Arabidopsis cells: cytosolic acidification and cellular oxidation, and the toxic mechanism may vary among cell types.

DOI： 10.1093/bbb/zbae092

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DOI： 10.1101/2024.07.05.602132

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Publisher：Cold Spring Harbor Laboratory  

Seasonal changes in spring induce flowering by expressing the florigen, FLOWERING LOCUS T (FT), in Arabidopsis. FT is expressed in unique phloem companion cells with unknown characteristics. The question of which genes are co-expressed with FT and whether they have roles in flowering remains elusive. Through tissue-specific translatome analysis, we discovered that under long-day conditions with the natural sunlight red/far-red ratio, the FT-producing cells express a gene encoding FPF1-LIKE PROTEIN 1 (FLP1). The master FT regulator, CONSTANS (CO), controls FLP1 expression, suggesting FLP1's involvement in the photoperiod pathway. FLP1 promotes early flowering independently of FT, is active in the shoot apical meristem, and induces the expression of SEPALLATA 3 (SEP3), a key E-class homeotic gene. Unlike FT, FLP1 facilitates inflorescence stem elongation. Our cumulative evidence indicates that FLP1 may act as a mobile signal. Thus, FLP1 orchestrates floral initiation together with FT and promotes inflorescence stem elongation during reproductive transitions.

DOI： 10.1101/2024.04.26.591289

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DOI： 10.1007/s10327-024-01191-3

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DOI： 10.1016/j.plantsci.2023.111840

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Language：English   Publishing type：Research paper (scientific journal)  

Dihydroxyacetone (DHA) occurs in wide-ranging organisms including plants and can undergo spontaneous conversion to methylglyoxal (MG). While the toxicity of MG to plants is well-known, the toxicity of DHA to plants remains to be elucidated. We investigated the effects of DHA and MG on Arabidopsis. Exogenous DHA at up to 10 m m did not affect the radicle emergence, the expansion of green cotyledons, the seedling growth, or the activity of glyoxalase II while DHA at 10 m m inhibited the root elongation and increased the activity of glyoxalase I. Exogenous MG at 1.0 m m inhibited these physiological responses and increased both activities. DHA at 10 m m increased the MG content in the roots. These results indicate that DHA is not so toxic as MG in Arabidopsis seeds and seedlings and suggest that the toxic effect of DHA at high concentrations is attributed to MG accumulation by the conversion to MG.

DOI： 10.1093/bbb/zbad109

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DOI： 10.1111/tpj.16281

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

DOI： 10.1021/acs.jafc.2c08479

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Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

<title>Abstract</title>Perception of pathogen-derived ligands by corresponding host receptors is a pivotal strategy in eukaryotic innate immunity. In plants, this is complemented by circadian anticipation of infection timing, promoting basal resistance even in the absence of pathogen threat. Here, we report that trichomes, hair-like structures on the epidermis, directly sense external mechanical forces, including raindrops, to anticipate pathogen infections in <italic>Arabidopsis thaliana</italic>. Exposure of leaf surfaces to mechanical stimuli initiates the concentric propagation of intercellular calcium waves away from trichomes to induce defence-related genes. Propagating calcium waves enable effective immunity against pathogenic microbes through the CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 3 (CAMTA3) and mitogen-activated protein kinases. We propose an early layer of plant immunity in which trichomes function as mechanosensory cells that detect potential risks.

DOI： 10.1038/s41467-022-28813-8

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Other Link： https://www.nature.com/articles/s41467-022-28813-8

Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Association for the Advancement of Science (AAAS)  

The ubiquitin-proteasome system is vital to hormone-mediated developmental and stress responses in plants. Ubiquitin ligases target hormone-specific transcriptional activators (TAs) for degradation, but how TAs are processed by proteasomes remains unknown. We report that in Arabidopsis , the salicylic acid– and ethylene-responsive TAs, NPR1 and EIN3, are relayed from pathway-specific ubiquitin ligases to proteasome-associated HECT-type UPL3/4 ligases. Activity and stability of NPR1 were regulated by sequential action of three ubiquitin ligases, including UPL3/4, while proteasome processing of EIN3 required physical handover between ethylene-responsive SCF ^EBF2 and UPL3/4 ligases. Consequently, UPL3/4 controlled extensive hormone-induced developmental and stress-responsive transcriptional programs. Thus, our findings identify unknown ubiquitin ligase relays that terminate with proteasome-associated HECT-type ligases, which may be a universal mechanism for processive degradation of proteasome-targeted TAs and other substrates.

DOI： 10.1126/sciadv.abn4466

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Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1111/pce.14373

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pce.14373

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

SUMMARY

Increasing the vegetative growth period of crops can increase biomass and grain yield. In rice (Oryza sativa), the concentration of trans ‐zeatin, an active cytokinin, was high in the leaves during vegetative growth and decreased rapidly upon induction of florigen expression, suggesting that this hormone is involved in the regulation of the vegetative phase. To elucidate whether exogenous cytokinin application influences the length of the vegetative phase, we applied 6‐benzylaminopurine (BAP) to rice plants at various developmental stages. Our treatment delayed flowering time by 8–9 days when compared with mock‐treated rice plants, but only at the transition stage when the flowering signals were produced. Our observations also showed that flowering in the paddy field is delayed by thidiazuron, a stable chemical that mimics the effects of cytokinin. The transcript levels of florigen genes Heading date 3a (Hd3a) and Rice Flowering locus T1 (RFT1) were significantly reduced by the treatment, but the expression of Early heading date 1 (Ehd1), a gene found directly upstream of the florigen genes, was not altered. In maize (Zea mays), similarly, BAP treatment increased the vegetative phage by inhibiting the expression of ZCN8, an ortholog of Hd3a. We showed that cytokinin treatment induced the expression of two type‐A response regulators (OsRR1 and OsRR2) which interacted with Ehd1, a type‐B response regulator. We also observed that cytokinin did not affect flowering time in ehd1 knockout mutants. Our study indicates that cytokinin application increases the duration of the vegetative phase by delaying the expression of florigen genes in rice and maize by inhibiting Ehd1.

DOI： 10.1111/tpj.15760

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.15760

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To acclimate to waterlogged conditions, wetland plants form a barrier to radial oxygen loss (ROL) that can enhance oxygen transport to the root apex. We hypothesized that one or more hormones are involved in the induction of the barrier and searched for such hormones in rice. We previously identified 98 genes that were tissue-specifically upregulated during ROL barrier formation in rice. The RiceXPro database showed that most of these genes were highly enhanced by exogenous abscisic acid (ABA). We then examined the effect of ABA on ROL barrier formation by using an ABA biosynthesis inhibitor (fluridone, FLU), by applying exogenous ABA and by examining a mutant with a defective ABA biosynthesis gene (osaba1). FLU suppressed barrier formation in a stagnant solution that mimics waterlogged soil. Under aerobic conditions, rice does not naturally form a barrier, but 24 h of ABA treatment induced barrier formation. osaba1 did not form a barrier under stagnant conditions, but the application of ABA rescued the barrier. In parallel with ROL barrier formation, suberin lamellae formed in the exodermis. These findings strongly suggest that ABA is an inducer of suberin lamellae formation in the exodermis, resulting in an ROL barrier formation in rice.

DOI： 10.1111/nph.17751

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media {LLC}  

It is known that rice roots take up cadmium (Cd) via the symplastic route mediated by membrane-bound mineral transporters. Here we provide evidence that apoplastic bypass flow is another Cd uptake route in rice. High concentrations of Cd rendered apoplastic bypass flow rate increased in rice seedlings. These concentrations of Cd compromised membrane integrity in the root meristem and transition zone. Polyethleneglycol and proline inhibited the Cd-induced apoplastic bypass flow and Cd transfer to the shoots. Loss-of-function mutant of the Cd uptake transporter, nramp5, showed Cd transport to the shoot comparable to the wild type. At a low Cd concentration, increased apoplastic bypass flow rate by NaCl stress resulted in an elevation of Cd transport to shoots both in the wildtype and nramp5. These observations indicate that apoplastic bypass flow in roots carries Cd transport leading to xylem loading of Cd in addition to the symplastic pathway mediated by mineral transporters under stressed conditions.

DOI： 10.1007/s10265-021-01319-y

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press ({OUP})  

Plastids are involved in phytohormone metabolism as well as photosynthesis. However, the mechanism by which plastid retrograde signals and phytohormones cooperatively regulate plastid biogenesis remains elusive. Here, we investigated the effects of an inhibitor and a mutation that generate biogenic plastid signals on phytohormones, and vice versa. Inhibition of plastid biogenesis by norflurazon (NF)-treatment and the plastid protein import2 (ppi2) mutation caused a decrease in salicylic acid (SA) and jasmonic acid (JA). This effect can be attributed in part to the altered expression of genes involved in the biosynthesis and metabolism of SA and JA. However, SA-dependent induction of the PATHOGENESIS-RELATED1 gene was virtually unaffected in NF-treated plants and the ppi2 mutant. Instead, the level of chlorophyll in these plants was partially restored by exogenous application of SA. Consistent with this observation, the levels of some photosynthesis-associated proteins increased in the ppi2 and NF-treated plants in response to SA treatment. This regulation in true leaves seems to occur at post-transcriptional level, since SA treatment did not induce the expression of photosynthesis associated genes. In salicylic acid induction deficient 2 and lesions simulating disease resistance 1 mutants, endogenous SA regulates the accumulation of photosynthesis-associated proteins through transcriptional and post-transcriptional mechanisms. These data indicate that SA acts antagonistically to the inhibition of plastid biogenesis by promoting the accumulation of photosynthesis-associated proteins in Arabidopsis, suggesting a possible link between SA and biogenic plastid signaling.

DOI： 10.1093/pcp/pcab128

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DOI： 10.1016/j.jplph.2021.153448

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Rhizoctonia solani is a soil-borne necrotrophic fungus that causes sheath blight in grasses. The basal resistance of compatible interactions between R. solani and rice is known to be modulated by some WRKY transcription factors (TFs). However, genes and defense responses involved in incompatible interaction with R. solani remain unexplored, because no such interactions are known in any host plants. Recently, we demonstrated that Bd3-1, an accession of the model grass Brachypodium distachyon, is resistant to R. solani and, upon inoculation with the fungus, undergoes rapid induction of genes responsive to the phytohormone salicylic acid (SA) that encode the WRKY TFs BdWRKY38 and BdWRKY44. Here, we show that endogenous SA and these WRKY TFs positively regulate this accession-specific R. solani resistance. In contrast to a susceptible accession (Bd21), the infection process in the resistant accessions Bd3-1 and Tek-3 was suppressed at early stages before the development of fungal biomass and infection machinery. A comparative transcriptome analysis during pathogen infection revealed that putative WRKY-dependent defense genes were induced faster in the resistant accessions than in Bd21. A gene regulatory network (GRN) analysis based on the transcriptome dataset demonstrated that BdWRKY38 was a GRN hub connected to many target genes specifically in resistant accessions, whereas BdWRKY44 was shared in the GRNs of all three accessions. Moreover, overexpression of BdWRKY38 increased R. solani resistance in Bd21. Our findings demonstrate that these resistant accessions can activate an incompatible host response to R. solani, and BdWRKY38 regulates this response by mediating SA signaling.

DOI： 10.1111/tpj.14976

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Plant phenotypes caused by mineral deficiencies differ depending on growth conditions. We recently reported that the growth of Arabidopsis thaliana was severely inhibited on MGRL-based zinc (Zn)-deficient medium but not on Murashige-Skoog-based Zn-deficient medium. Here, we explored the underlying reason for the phenotypic differences in Arabidopsis grown on the different media. The root growth and chlorophyll contents reduced by Zn deficiency were rescued by the addition of extra manganese (Mn) during short-term growth (10 or 14 d). However, this treatment did not affect the growth recovery after long-term growth (38 d). To investigate the reason for plant recovery from Zn deficiency, we performed the RNA-seq analysis of the roots grown on the Zn-basal medium and the Zn-depleted medium with/without additional Mn. Principal component analysis of the RNA-seq data showed that the gene expression patterns of plants on the Zn-basal medium were similar to those on the Zn-depleted medium with Mn, whereas those on the Zn-depleted medium without Mn were different from the others. The expression of several transcription factors and reactive oxygen species (ROS)-related genes was upregulated in only plants on the Zn-depleted medium without Mn. Consistent with the gene expression data, ROS accumulation in the roots grown on this medium was higher than those grown in other conditions. These results suggest that plants accumulate ROS and reduce their biomass under undesirable growth conditions, such as Zn depletion. Taken together, this study shows that the addition of extra Mn to the Zn-depleted medium induces transcriptional changes in ROS-related genes, thereby alleviating short-term growth inhibition due to Zn deficiency.

DOI： 10.1093/pcp/pcaa094

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Peel degreening is an important aspect of fruit ripening in many citrus fruit, and previous studies have shown that it can be advanced by ethylene treatment or by low-temperature storage. However, the important regulators and pathways involved in natural peel degreening remain largely unknown. To determine how natural peel degreening is regulated in lemon fruit (Citrus limon), we studied transcriptome and physiochemical changes in the flavedo in response to ethylene treatment and low temperatures. Treatment with ethylene induced rapid peel degreening, which was strongly inhibited by the ethylene antagonist, 1-methylcyclopropene (1-MCP). Compared with 25 ºC, moderately low storage temperatures of 5-20 °C also triggered peel degreening. Surprisingly, repeated 1-MCP treatments failed to inhibit the peel degreening induced by low temperature. Transcriptome analysis revealed that low temperature and ethylene independently regulated genes associated with chlorophyll degradation, carotenoid metabolism, photosystem proteins, phytohormone biosynthesis and signalling, and transcription factors. Peel degreening of fruit on trees occurred in association with drops in ambient temperature, and it coincided with the differential expression of low temperature-regulated genes. In contrast, genes that were uniquely regulated by ethylene showed no significant expression changes during on-tree peel degreening. Based on these findings, we hypothesize that low temperature plays a prominent role in regulating natural peel degreening independently of ethylene in citrus fruit.

DOI： 10.1093/jxb/eraa206

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Informa {UK} Limited  

Reactive oxygen species and nitric oxide (NO•) concomitantly play essential roles in guard cell signaling. Studies using catalase mutants have revealed that the inducible and constitutive elevations of intracellular hydrogen peroxide (H2O2) have different roles: only the inducible H2O2 production transduces the abscisic acid (ABA) signal leading stomatal closure. However, the involvement of inducible or constitutive NO• productions, if exists, in this process remains unknown. We studied H2O2 and NO• mobilization in guard cells of catalase mutants. Constitutive H2O2 level was higher in the mutants than that in wild type, but constitutive NO• level was not different among lines. Induced NO• and H2O2 levels elicited by ABA showed a high correlation with each other in all lines. Furthermore, NO• levels increased by exogenous H2O2 also showed a high correlation with stomatal aperture size. Our results demonstrate that ABA-induced intracellular H2O2 accumulation triggers NO• production leading stomatal closure. ABBREVIATIONS: ABA: abscisic acid; CAT: catalase; cGMP: cyclic guanosine monophosphate; DAF-2DA: 4,5-diaminofluorescein-2 diacetate; H2DCF-DA: 2',7'-dichlorodihydrofluorescein diacetate; MeJA: methyljasmonate; NOS: nitric oxide synthetase; NR: nitrate reductase; POX: peroxidase; ROS: reactive oxygen species; SNAP: S-nitroso-N-acetyl-DL-penicillamine; SNP: sodium nitroprusside; NOX: NADP(H) oxidase.

DOI： 10.1080/09168451.2020.1743168

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier {BV}  

DOI： 10.1016/j.chemosphere.2020.125933

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DOI： 10.1007/s10265-019-01156-0

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DOI： 10.1270/jsbbs.19030

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DOI： 10.1111/tpj.14456

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Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.postharvbio.2019.110949

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DOI： 10.1186/s12870-018-1491-2

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

Plants closing stomata in the presence of harmful gases is believed to be a stress avoidance mechanism. SO2 , one of the major airborne pollutants, has long been reported to induce stomatal closure, yet the mechanism remains unknown. Little is known about the stomatal response to airborne pollutants besides O3 . SLOW ANION CHANNEL-ASSOCIATED 1 (SLAC1) and OPEN STOMATA 1 (OST1) were identified as genes mediating O3 -induced closure. SLAC1 and OST1 are also known to mediate stomatal closure in response to CO2 , together with RESPIRATORY BURST OXIDASE HOMOLOGs (RBOHs). The overlaying roles of these genes in response to O3 and CO2 suggested that plants share their molecular regulators for airborne stimuli. Here, we investigated and compared stomatal closure event induced by a wide concentration range of SO2 in Arabidopsis through molecular genetic approaches. O3 - and CO2 -insensitive stomata mutants did not show significant differences from the wild type in stomatal sensitivity, guard cell viability, and chlorophyll content revealing that SO2 -induced closure is not regulated by the same molecular mechanisms as for O3 and CO2 . Nonapoptotic cell death is shown as the reason for SO2 -induced closure, which proposed the closure as a physicochemical process resulted from SO2 distress, instead of a biological protection mechanism.

DOI： 10.1111/pce.13406

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：{MDPI} {AG}  

Cyclic nucleotide-gated channels (CNGCs) have been postulated to contribute significantly in plant development and stress resistance. However, their electrophysiological properties remain poorly understood. Here, we characterized barley CNGC2-3 (HvCNGC2-3) by the two-electrode voltage-clamp technique in the Xenopus laevis oocyte heterologous expression system. Current was not observed in X. laevis oocytes injected with HvCNGC2-3 complementary RNA (cRNA) in a bathing solution containing either Na⁺ or K⁺ solely, even in the presence of 8-bromoadenosine 3',5'-cyclic monophosphate (8Br-cAMP) or 8-bromoguanosine 3',5'-cyclic monophosphate (8Br-cGMP). A weakly voltage-dependent slow hyperpolarization-activated ion current was observed in the co-presence of Na⁺ and K⁺ in the bathing solution and in the presence of 10 µM 8Br-cAMP, but not 8Br-cGMP. Permeability ratios of HvCNGC2-3 to K⁺, Na⁺ and Cl- were determined as 1:0.63:0.03 according to reversal-potential analyses. Amino-acid replacement of the unique ion-selective motif of HvCNGC2-3, AQGL, with the canonical motif, GQGL, resulted in the abolition of the current. This study reports a unique two-ion-dependent activation characteristic of the barley CNGC, HvCNGC2-3.

DOI： 10.3390/plants7030061

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Tokyo  

DOI： 10.1007/s10265-017-0972-y

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DOI： 10.1111/nph.14849

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Other Link： http://orcid.org/0000-0002-4481-6189

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DOI： 10.1007/s10265-017-0969-6

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DOI： 10.1093/jxb/erw289

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DOI： 10.1038/s41598-017-03907-2

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DOI： 10.1093/treephys/tpw134

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DOI： 10.1515/bot-2016-0056

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Language：Chinese   Publishing type：Research paper (scientific journal)   Publisher：Crop Science Society of Japan  

DOI： 10.1626/jcs.86.367

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DOI： 10.1080/09168451.2017.1308244

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DOI： 10.1093/pcp/pcw157

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DOI： 10.1093/pcp/pcw102

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DOI： 10.1007/s10811-015-0759-2

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DOI： 10.1016/j.plaphy.2015.12.005

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DOI： 10.1007/s10265-015-0757-0

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DOI： 10.3390/proteomes4010001

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DOI： 10.1093/jxb/erw071

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DOI： 10.1080/09168451.2015.1045827

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DOI： 10.1111/plb.12321

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DOI： 10.1093/jxb/erv264

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DOI： 10.1093/pcp/pcv051

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DOI： 10.1371/journal.pone.0123308

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/s150202354

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.chemosphere.2014.07.011

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DOI： 10.1093/jxb/erv086

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DOI： 10.1111/tpj.12996

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DOI： 10.1146/annurev-arplant-043014-114707

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DOI： 10.1111/plb.12175

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DOI： 10.1016/j.jplph.2014.02.003

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DOI： 10.1093/pcp/pcu003

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DOI： 10.1016/j.jplph.2013.10.001

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Research Foundation  

DOI： 10.3389/fpls.2013.00472

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DOI： 10.1104/pp.113.224055

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DOI： 10.1104/pp.113.225045

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DOI： 10.1104/pp.113.223826

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DOI： 10.1016/j.jplph.2013.03.011

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/bbb.120928

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DOI： 10.1111/j.1438-8677.2012.00671.x

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Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

DOI： 10.1007/s00344-012-9291-7

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DOI： 10.1111/jipb.12057

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/bbb.120980

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1271/bbb.120384

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DOI： 10.1271/bbb.120228

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/jbt.21423

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1271/bbb.120233

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jplph.2012.03.006

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1104/pp.112.195578

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1271/bbb.111000

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/jxb/err369

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Authorship：Corresponding author   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jplph.2011.05.006

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Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.jplph.2011.06.002

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Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.jplph.2011.05.004

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Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1111/j.1365-3040.2011.02385.x

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/bbb.110344

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.asr.2011.05.017

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Publishing type：Research paper (scientific journal)   Publisher：Informa UK Limited  

DOI： 10.4161/psb.6.7.15439

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Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

DOI： 10.1104/pp.111.172254

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Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1111/j.1365-3040.2010.02253.x

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Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

DOI： 10.1104/pp.110.162750

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Authorship：Lead author   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s10265-011-0435-9

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/bbb.100513

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/bbb.100393

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pcq145

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pcq131

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/s100706290

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pcq016

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pcq001

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/bbb.100340

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1271/bbb.90244

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pcp066

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Landes Bioscience  

DOI： 10.4161/psb.4.2.7537

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Authorship：Lead author  

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Publisher：The Japanese Society of Plant Physiologists  

In plants, metyl jasmonate (MeJA), which mediates various plant defense response, as well as abscisic acid (ABA) induces stomatal closure. It has been shown that there are various signaling factors in MeJA signaling in guard cells. RCN1, an Arabidopsis protein phosphatase 2A (PP2A) A subunit, is involved in auxin signaling in roots and ABA signaling in guard cells. Previously, our study suggested that RCN1 functions as a positive regulator in MeJA signaling in guard cells. RCN1 is required for MeJA regulation of reactive oxygen species production and inward-rectifying potassium ion channel currents in guard cells. <br> In order to gain further information about MeJA signaling in guard cells, we evaluated roles of RCN1 in MeJA regulation of nitric oxide production, calcium ion channel currents, and S-type anion channel currents in guard cells. Our results suggest a novel model of MeJA signaling in Arabidopsis guard cells.

DOI： 10.14841/jspp.2009.0.0451.0

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1271/bbb.80407

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pcn106

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1371/journal.pbio.0040327

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1515/znc-2005-9-1017

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1104/pp.104.042069

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/emboj/cdg277

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1104/pp.012187

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1271/bbb.66.1069

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1105/tpc.13.11.2513

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pce176

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pce150

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1046/j.1529-8817.2000.00050.x

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pcd003

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Publishing type：Research paper (scientific journal)  

DOI： 10.1046/j.1529-8817.2000.00071.x

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/bbb.62.986

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Authorship：Lead author   Publishing type：Research paper (scientific journal)  

DOI： 10.1104/pp.113.3.833

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

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Language：Japanese  

J-GLOBAL

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Language：English   Publishing type：Research paper, summary (international conference)  

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DOI： 10.1002/9781118764374.ch3

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J-GLOBAL

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Language：Japanese   Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/kagakutoseibutsu.46.440

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Other Link： https://jlc.jst.go.jp/DN/JALC/00316431291?from=CiNii

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Language：English   Publishing type：Research paper, summary (international conference)  

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Language：English   Publisher：Japanese Society of Plant Physiologists  

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Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=183810

Language：English  

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Language：English  

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Language：Japanese   Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

DOI： 10.1271/kagakutoseibutsu1962.36.66

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Other Link： https://jlc.jst.go.jp/DN/JALC/00050986040?from=CiNii

Language：Japanese  

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Language：English  

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Language：Japanese  

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Event date： 2023.10.13 - 2023.10.15

Language：English   Presentation type：Poster presentation  

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Event date： 2023.3.10 - 2023.3.17

Language：Japanese   Presentation type：Poster presentation  

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Event date： 2022.7.9 - 2022.7.13

Language：English   Presentation type：Poster presentation  

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Event date： 2022.3.22 - 2022.3.24

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2022.3.22 - 2022.3.24

Language：English   Presentation type：Poster presentation  

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Event date： 2022.3.20 - 2022.3.24

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2020.9.19 - 2020.9.21

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2020.9.8 - 2020.9.10

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2020.7.27 - 2020.7.31

Language：English   Presentation type：Poster presentation  

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Event date： 2020.3.19 - 2020.3.21

Presentation type：Poster presentation  

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Event date： 2019.3.13 - 2019.3.15

Language：English   Presentation type：Poster presentation  

Venue：名古屋  

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Event date： 2019.3.13 - 2019.3.15

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：名古屋  

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Event date： 2019.3.13 - 2019.3.15

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：名古屋  

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Event date： 2019.3.13 - 2019.3.15

Language：English   Presentation type：Poster presentation  

Venue：名古屋  

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Event date： 2019.3.13 - 2019.3.15

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：名古屋  

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Event date： 2019.3.13 - 2019.3.15

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：名古屋  

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Event date： 2018.9.24 - 2018.9.27

Language：English   Presentation type：Symposium, workshop panel (nominated)  

Venue：Kobe  

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Event date： 2018.8.29 - 2018.8.31

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：神奈川  

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Event date： 2018.8.5 - 2018.8.10

Language：English   Presentation type：Poster presentation  

Venue：Monpelier, France  

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Event date： 2018.7.3

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Venue：大阪府吹田市  

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Event date： 2018.3.28 - 2018.3.30

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：札幌市  

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Event date： 2018.3.28 - 2018.3.30

Language：English   Presentation type：Oral presentation (general)  

Venue：札幌市  

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Event date： 2018.3.28 - 2018.3.30

Language：English   Presentation type：Oral presentation (general)  

Venue：札幌  

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Event date： 2018.3.19

Language：English   Presentation type：Oral presentation (invited, special)  

Venue：Changchun, Jilin, China  

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Event date： 2018.3.15 - 2018.3.18

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：名古屋  

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Event date： 2018.1.25

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Venue：岡山県倉敷市  

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Event date： 2017.6.26 - 2017.6.30

Language：English   Presentation type：Oral presentation (general)  

Venue：Matsue, Japan  

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Event date： 2017.6.24 - 2017.6.28

Language：English   Presentation type：Poster presentation  

Venue：Honolulu, Hawai, USA  

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Event date： 2017.6.24 - 2017.6.28

Language：English   Presentation type：Poster presentation  

Venue：Honolulu, Hawai, USA  

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Event date： 2016.3.18 - 2016.3.20

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：Morioka  

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Event date： 2015.9.22 - 2015.9.25

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：仙台  

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Event date： 2008.9.25

Presentation type：Oral presentation (general)  

Venue：高知県  

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Event date： 2008.4

Presentation type：Poster presentation  

Venue：高知市  

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Event date： 2008.4

Presentation type：Poster presentation  

Venue：高知市  

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Event date： 2008.2

Presentation type：Oral presentation (general)  

Venue：Langkawi, Malaysia  

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Event date： 2007.9.7 - 2007.9.9

Venue：野田市  

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Event date： 2007.8.19 - 2007.8.24

Venue：Toyama  

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Event date： 2007.6.26 - 2007.6.30

Venue：Valencia  

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Event date： 2007.6.26 - 2007.6.30

Venue：Valencia  

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Event date： 2007.3.29 - 2007.3.31

Venue：松山市  

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Event date： 2007.2.5 - 2007.2.6

Venue：Kitakyushu  

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Event date： 2006.11.22 - 2006.11.23

Venue：Osaka  

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Event date： 2006.3.15

Venue：Bangin, Malaysia  

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Event date： 2005.9.26 - 2005.9.28

Venue：Xiamen University, Xiamen, People’s Republic of China.  

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Event date： 2005.3.29 - 2005.3.30

Venue：札幌  

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Event date： 2005.2.21

Venue：Kuala Lumpur, Malaysia  

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Event date： 2004.5.11

Venue：San Diego, California, USA  

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Event date： 2004.5.11

Venue：San Diego, California, USA  

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Event date： 2004.4.7

Venue：横浜  

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Event date： 2004.3.27 - 2004.3.29

Venue：東京  

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Event date： 2004.3.26

Venue：東京  

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Event date： 2003.6.23 - 2003.6.28

Venue：Barcerona, Spain.  

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Event date： 2003.6

Venue：Madison, Wisconsin, USA  

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Event date： 2001.8

Venue：Madison, Wisconsin, USA  

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Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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