2021/07/12 更新

写真a

ミヨシ シンイチ
三好 伸一
MIYOSHI Shinichi
所属
医歯薬学域 教授
職名
教授
外部リンク

学位

  • 薬学修士 ( 岡山大学 )

  • 薬学博士 ( 大阪大学 )

研究キーワード

  • 蛋白質毒素

  • 環境微生物

  • ビブリオ

  • Vibrio

  • Protein toxin

  • Environmental microorganism

  • Protease

  • Bioremediation

  • プロテアーゼ

  • バイオレメディエーション

研究分野

  • ライフサイエンス / 薬系衛生、生物化学

  • ライフサイエンス / 細菌学

  • ライフサイエンス / 薬系衛生、生物化学

  • ライフサイエンス / 薬理学

  • ライフサイエンス / 衛生学、公衆衛生学分野:実験系を含む

  • ライフサイエンス / 衛生学、公衆衛生学分野:実験系を含まない

  • ライフサイエンス / 医療管理学、医療系社会学

  • ライフサイエンス / 医療管理学、医療系社会学

  • ライフサイエンス / 衛生学、公衆衛生学分野:実験系を含む

  • ライフサイエンス / 衛生学、公衆衛生学分野:実験系を含まない

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学歴

  • 岡山大学   Graduate School, Division of Pharmaceutical Sciences  

    - 1985年

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  • 岡山大学    

    - 1985年

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    国名: 日本国

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  • 岡山大学   Faculty of Pharmaceutical Science  

    - 1983年

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  • 岡山大学   薬学部   製薬化学

    - 1983年

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    国名: 日本国

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経歴

  • - Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2005年

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  • - 岡山大学医歯薬学総合研究科 教授

    2005年

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  • 国際協力事業団インド下痢症対策プロジェクト 短期専門家 未設定

    2002年

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  • 米国コロラド州立大学 生化学・分子生物学科 博士研究員 未設定

    1998年 - 1999年

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  • 米国コロラド州立大学 生化学・分子生物学科 博士研究員 未設定

    1994年 - 1995年

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所属学協会

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委員歴

  • 日本防菌防黴学会   防菌防黴誌編集委員  

    2011年   

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    団体区分:学協会

    日本防菌防黴学会

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  • 日本防菌防黴学会   理事  

    2011年   

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    団体区分:学協会

    日本防菌防黴学会

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  • 日本薬学会   日本薬学会学会賞第1次選考委員  

    2010年   

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    団体区分:学協会

    日本薬学会

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  • 日本薬学会   衛生薬学教科担当教員会議委員  

    2010年   

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    団体区分:学協会

    日本薬学会

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  • 日米医学協力研究会 コレラ・細菌性腸管感染症専門部会   研究員  

    2009年   

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    団体区分:学協会

    日米医学協力研究会 コレラ・細菌性腸管感染症専門部会

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  • 日本薬学会   日本薬学会第130年会組織委員  

    2009年   

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    団体区分:学協会

    日本薬学会

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  • 日本食品微生物学会   評議員  

    2008年   

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    団体区分:学協会

    日本食品微生物学会

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  • 日本細菌学会   評議員  

    2008年   

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    団体区分:学協会

    日本細菌学会

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  • 日本細菌学会   広報委員会委員  

    2008年   

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    団体区分:学協会

    日本細菌学会

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  • 日本薬学会   中国四国支部幹事  

    2007年 - 2008年   

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    団体区分:学協会

    日本薬学会

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  • 日本薬学会   環境・衛生部会試験法委員会微生物試験法専門委員会委員長  

    2007年   

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    団体区分:学協会

    日本薬学会

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  • 日本薬学会   環境・衛生部会試験法委員会委員  

    2007年   

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    団体区分:学協会

    日本薬学会

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  • 日本薬学会   フォーラム2007 衛生薬学・環境トキシコロジー実行委員会委員  

    2007年   

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    団体区分:学協会

    日本薬学会

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  • 日本薬学会   代議員  

    2007年   

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    団体区分:学協会

    日本薬学会

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  • 日本薬学会   放射薬学教科担当教員会議委員  

    2006年   

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    団体区分:学協会

    日本薬学会

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  • 毒素シンポジウム   運営委員  

    2005年 - 2007年   

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    団体区分:学協会

    毒素シンポジウム

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  • 日本防菌防黴学会   Biocontrol Science編集委員  

    2005年   

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    団体区分:学協会

    日本防菌防黴学会

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  • 日本防菌防黴学会   評議員  

    2005年   

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    団体区分:学協会

    日本防菌防黴学会

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  • 日本薬学会   環境・衛生部会試験法委員会微生物試験法専門委員会専門委員  

    2003年   

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    団体区分:学協会

    日本薬学会

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  • 日本薬学会   中国四国支部幹事  

    2001年 - 2002年   

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    団体区分:学協会

    日本薬学会

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  • 日本細菌学会   中国四国支部評議員  

    1999年   

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    団体区分:学協会

    日本細菌学会

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書籍等出版物

  • 薬学領域の環境衛生学

    廣川書店  2017年  ( ISBN:9784567476706

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  • 有害微生物の制御と管理−現場対応への実践的な取り組み−

    テクノシステム  2016年  ( ISBN:9784924728776

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  • 衛生試験法・注解2015

    金原出版  2015年 

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  • 菌・カビを知る・防ぐ・60の知恵

    化学同人  2015年  ( ISBN:9784759815993

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  • 標準微生物学 改訂第12版

    医学書院  2015年 

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  • 微生物の簡易迅速検査法

    テクノシステム  2013年 

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  • Handbook of Proteolytic Enzymes (Third Edition)

    Elsevier Academic Press  2013年 

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  • 腸炎ビブリオ 第Ⅳ集

    近代出版  2013年 

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  • シンプル微生物学 改訂第5版

    南江堂  2011年 

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  • 病原菌の今日的意味 改訂4版

    医薬ジャーナル  2011年 

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  • 食品微生物学辞典

    中央法規出版  2010年 

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  • 環境衛生の科学(第2版)

    三共出版  2010年 

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  • 衛生試験法・注解2010

    金原出版  2010年 

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  • 薬毒物分析学辞典

    廣川書店  2009年 

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  • Comprehensive sourcebook of bacterial protein toxins, Third Edition

    Elsevier Academic Press  2006年 

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  • 衛生試験法・注解2005

    金原出版  2005年 

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  • 毒物・中毒用語辞典

    化学同人  2005年 

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  • Handbook of Proteolytic Enzymes (Second Edition)

    Elsevier Academic Press  2004年 

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  • 細菌毒素ハンドブック

    サイエンスフォーラム  2002年 

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  • 事件からみた毒-トリカブトからサリンまで

    化学同人  2001年 

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  • 環境衛生の科学

    三共出版  2001年 

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MISC

  • Antibacterial Activities of Surfactants in the Laundry Detergents and Isolation of the Surfactant Resistant Aquatic Bacteria

    Yoko Maehara, Shin-Ichi Miyoshi

    BIOCONTROL SCIENCE   22 ( 4 )   229 - 232   2017年12月

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    記述言語:英語   出版者・発行元:SOC ANTIBACTERIAL & ANTIFUNGAL AGENTS, JAPAN  

    Linear alkylbenzene sulfonate (LAS) and polyoxyethylene lauryl ether (POLE) are major surfactants contained in the laundry detergents. In the present study, the antibacterial activities of the surfactants to aquatic microorganisms were compared. When freshwater samples from a small river in Okayama city were treated with each of the surfactants, only LAS showed the significant antibacterial activity. Several strains, which survived after the treatment with 2.0% LAS, were isolated and identified by sequencing of 16S rDNA. All strains were classified into the family Enterobacteriaceae. However, this family was not a major member of the aquatic microflora, suggesting that the bacteria in Enterobacteriaceae have a common property of LAS-resistance in the river water.

    DOI: 10.4265/bio.22.229

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  • Comparative genome analysis of VSP-II and SNPs reveals heterogenic variation in contemporary strains of Vibrio cholerae O1 isolated from cholera patients in Kolkata, India

    Daisuke Imamura, Masatomo Morita, Tsuyoshi Sekizuka, Tamaki Mizuno, Taichiro Takemura, Tetsu Yamashiro, Goutam Chowdhury, Gururaja P. Pazhani, Asish K. Mukhopadhyay, Thandavarayan Ramamurthy, Shin-ichi Miyoshi, Makoto Kuroda, Sumio Shinoda, Makoto Ohnishi

    PLOS NEGLECTED TROPICAL DISEASES   11 ( 2 )   1600 - 1606   2017年2月

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    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Cholera is an acute diarrheal disease and a major public health problem in many developing countries in Asia, Africa, and Latin America. Since the Bay of Bengal is considered the epi-center for the seventh cholera pandemic, it is important to understand the genetic dynamism of Vibrio cholerae from Kolkata, as a representative of the Bengal region. We analyzed whole genome sequence data of V. cholerae O1 isolated from cholera patients in Kolkata, India, from 2007 to 2014 and identified the heterogeneous genomic region in these strains. In addition, we carried out a phylogenetic analysis based on the whole genome single nucleotide polymorphisms to determine the genetic lineage of strains in Kolkata. This analysis revealed the heterogeneity of the Vibrio seventh pandemic island (VSP)-II in Kolkata strains. The ctxB genotype was also heterogeneous and was highly related to VSP-II types. In addition, phylogenetic analysis revealed the shifts in predominant strains in Kolkata. Two distinct lineages, 1 and 2, were found between 2007 and 2010. However, the proportion changed markedly in 2010 and lineage 2 strains were predominant thereafter. Lineage 2 can be divided into four sublineages, I, II, III and IV. The results of this study indicate that lineages 1 and 2-I were concurrently prevalent between 2007 and 2009, and lineage 2-III observed in 2010, followed by the predominance of lineage 2-IV in 2011 and continued until 2014. Our findings demonstrate that the epidemic of cholera in Kolkata was caused by several distinct strains that have been constantly changing within the genetic lineages of V. cholerae O1 in recent years.

    DOI: 10.1371/journal.pntd.0005386

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  • Regulation systems of protease and hemolysin production in Vibrio vulnificus

    Abdelaziz Elgaml, Shin-ichi Miyoshi

    MICROBIOLOGY AND IMMUNOLOGY   61 ( 1 )   1 - 11   2017年1月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:WILEY-BLACKWELL  

    Vibrio vulnificus is a gram-negative halophilic estuarine bacterium. It is an opportunistic human pathogen causing rapidly progressive fatal septicemia and necrotizing wound infection. This species also causes hemorrhagic septicemia called vibriosis in cultured eels. A range of virulence factors have been proposed to play a role in pathogenesis of the bacterium during human and/or eel infection. Among these factors, the metalloprotease (V. vulnificus protease: VVP) and the cytolytic toxin (V. vulnificus hemolysin: VVH) are significantly important. VVP elicits the characteristic edematous and hemorrhagic skin damage. On the other hand, VVH exhibits powerful hemolytic and cytolytic activities and it also contributes to the bacterial invasion from the intestine to the blood stream. In addition, a few V. vulnificus strains isolated from diseased eels were recently found to produce a serine protease termed as V. vulnificus serine protease (VvsA) instead of VVP. Similar to VVP, VvsA may also possess various toxic activities such as collagenolytic, cytotoxic and edema-forming activity. In this review, we clarify the regulation of V. vulnificus VVP, VVH and VvsA in terms of expression at mRNA and protein level. The explanation is given on the basis of quorum sensing system, which is dependent on the bacterial cell density. In addition, we also outline the role of environmental factors and global regulators, such as histone-like nucleoid structuring protein, cyclic AMP receptor protein, RpoS, HlyU, Fur, ToxRS, AphB and LeuO, in this regulation. Altogether, we compiled the cumulative impact of these regulatory systems on the pathogenicity of V. vulnificus.

    DOI: 10.1111/1348-0421.12465

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  • 食品・医薬品・環境分野等の微生物試験法および微生物汚染の制御に関する最近の話題7 「衛生試験法・注解2015」収載微生物試験法

    三好伸一

    防菌防黴   45 ( 5 )   277 - 279   2017年

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  • Regulation of Vibrio mimicus metalloprotease (VMP) production by the quorum-sensing master regulatory protein, LuxR

    El-Shaymaa Abdel-Sattar, Shin-ichi Miyoshi, Abdelaziz Elgaml

    JOURNAL OF BASIC MICROBIOLOGY   56 ( 10 )   1051 - +   2016年10月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Vibrio mimicus is an estuarine bacterium, while it can cause severe diarrhea, wound infection, and otitis media in humans. This pathogen secretes a relatively important toxin named V. mimicus metalloprotease (VMP). In this study, we clarified regulation of the VMP production according to the quorum-sensing master regulatory protein named LuxR. First, the full length of luxR gene, encoding LuxR, was detected in V. mimicus strain E-37, an environmental isolate. Next, the putative consensus binding sequence of LuxR protein could be detected in the upstream (promoter) region of VMP encoding gene, vmp. Finally, the effect of disruption of luxR gene on the expression of vmp and production of VMP was evaluated. Namely, the expression of vmp was significantly diminished by luxR disruption and the production of VMP was severely altered. Taken together, here we report that VMP production is under the positive regulation of the quorum-sensing master regulatory protein, LuxR.

    DOI: 10.1002/jobm.201600002

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  • Indolo[3,2-b]quinoline Derivatives Suppressed the Hemolytic Activity of Beta-Pore Forming Toxins, Aerolysin-Like Hemolysin Produced by Aeromonas sobria and Alpha-Hemolysin Produced by Staphylococcus aureus

    Eizo Takahashi, Chiaki Fujinami, Teruo Kuroda, Yasuo Takeuchi, Shin-ichi Miyoshi, Sakae Arimoto, Tomoe Negishi, Keinosuke Okamoto

    BIOLOGICAL & PHARMACEUTICAL BULLETIN   39 ( 1 )   114 - 120   2016年1月

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    記述言語:英語   出版者・発行元:PHARMACEUTICAL SOC JAPAN  

    In an attempt to discover inhibitory compounds against pore-forming toxins, some of the major toxins produced by bacteria, we herein examined the effects of four kinds of indolo[3,2-b]quinoline derivatives on hemolysis induced by the aerolysin-like hemolysin (ALH) of Aeromonas sobria and also by the alpha-hemolysin of Staphylococcus aureus. The results showed that hemolysis induced by ALH was significantly reduced by every derivative, while that induced by alpha-hemolysis was significantly reduced by three out of the four derivatives. However, the degrees of reduction induced by these derivatives were not uniform. Each derivative exhibited its own activity to inhibit the respective hemolysin. Compounds 1 and 2, which possessed the amino group bonding the naphthalene moiety at the C-11 position of indolo[3,2-b]quinoline, had strong inhibitory effects on the activity of ALH. Compound 4 which consisted of benzofuran and quinoline had strong inhibitory effects on the activity of alpha-hemolysin. These results indicated that the amino group bonding the naphthalene moiety of compounds 1 and 2 assisted in their ability to inhibit ALH activity, while the oxygen atom at the 10 position of compound 4 strengthened its interaction with alpha-hemolysin. These compounds also suppressed the hemolytic activity of the supernatant of A. sobria or A. hydrophila, suggesting that these compounds were effective at the site of infection of these bacteria.

    DOI: 10.1248/bpb.b15-00708

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  • Role of the Histone-Like Nucleoid Structuring Protein (H-NS) in the Regulation of Virulence Factor Expression and Stress Response in Vibrio vulnificus

    Abdelaziz Elgaml, Shin-Ichi Miyoshi

    BIOCONTROL SCIENCE   20 ( 4 )   263 - 274   2015年12月

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    記述言語:英語   出版者・発行元:SOC ANTIBACTERIAL & ANTIFUNGAL AGENTS, JAPAN  

    Temperature is one of the important parameters regulating the expression of virulence factors in bacteria. The global regulator, a histone-like nucleoid structuring protein (H-NS), is known to play a crucial role in this regulation. In the present study, we first clarified the role of H-NS in the temperature-dependent regulation of virulence factor production in Vibrio vulnificus, including that of the cytolytic toxin (V vulnificus hemolysin: VVH) and the proteolytic enzyme (V. vulnificus protease: VVP). The expression of hns itself was subjected to temperature regulation, where hns was expressed more at 26 degrees C than at 37 degrees C. VVH production and the expression of its gene vvhA were increased by disruption of the hns gene. H-NS appeared to affect the vvhA expression by the well-documented transcriptional silencing mechanism. On the other hand, hns disruption resulted in the reduction of VVP production and the expression of its gene vvpE. H-NS was suggested to positively regulate vvpE expression through the increase in the level of the rpoS mRNA. Moreover, H-NS was found to contribute to the survival of V vulnificus in stressful environments. When compared to the wild type strain, the hns mutant exhibited reduced survival rates when subjected to acidic pH, hyperosmotic and oxidative stress.

    DOI: 10.4265/bio.20.263

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  • Presence of Nitric Oxide-Sensing Systems in the Human Pathogen Vibrio vulnificus

    Abdelaziz Elgaml, Shin-ichi Miyoshi

    BIOCONTROL SCIENCE   20 ( 3 )   199 - 203   2015年9月

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    記述言語:英語   出版者・発行元:SOC ANTIBACTERIAL & ANTIFUNGAL AGENTS, JAPAN  

    Vibrio vulnificus is a halophilic estuarine bacterium, but this species causes fatal septicemia in humans. V. vulnificus may encounter many kinds of stresses either in the natural environment or in the human body. One of the striking stresses is the exposure to the reactive oxygen species including nitric oxide (NO). The present study revealed that NO could participate in the regulation of the V. vulnificus community behavior. When the bacterium was cultivated in the presence of sub-lethal doses of an NO donor, the expression of the genes encoding NO-detoxifying enzymes was significantly increased. The NO donor was also found to cause significant increase in production of a metalloprotease, a putative virulence factor, by the bacterium.

    DOI: 10.4265/bio.20.199

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  • Development of a real-time loop-mediated isothermal amplification assay for the sensitive and rapid detection of Listeria monocytogenes

    L. Ye, Y. Li, J. Zhao, Z. Zhang, H. Meng, H. Yan, S. -i. Miyoshi, L. Shi

    LETTERS IN APPLIED MICROBIOLOGY   61 ( 1 )   85 - 90   2015年7月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    A real-time loop-mediated isothermal amplification (RealAmp) assay for the detection of Listeria was developed. The RealAmp assay, using primers specific for the hemolysin-encoding hlyA gene, was verified using Listeria monocytogenes strains (n=58) from different regions of the world. Both the sensitivity and specificity of the RealAmp assay were high. The RealAmp assay could detect 10(3)CFUml(-1) within 30min. A comparative evaluation of the RealAmp assay, the API Listeria assay, and the real-time PCR assay revealed that the RealAmp assay is simpler, faster, and has a higher specificity than the other two assays.
    Significance and Impact of the StudyConventional culture and molecular detection methods are always time consuming and require a specific laboratory infrastructure, thereby restricting their use for the rapid detection and diagnosis of pathogens. A real-time loop-mediated isothermal amplification (RealAmp) assay performed by ESEtube scanner to rapidly detect Listeria monocytogenes isolated from food was developed. The results showed that the RealAmp assay using the tube scanner was more efficient and precise than the conventional API Listeria assay and the real-time PCR assay.

    DOI: 10.1111/lam.12429

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  • International Collaborative Research on Infectious Diseases by Japanese Universities and Institutes in Asia and Africa, with a Special Emphasis on J-GRID

    Sumio Shinoda, Daisuke Imamura, Tamaki Mizuno, Shin-Ichi Miyoshi, Thandavrayan Ramamurthy

    BIOCONTROL SCIENCE   20 ( 2 )   77 - 89   2015年6月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:SOC ANTIBACTERIAL & ANTIFUNGAL AGENTS, JAPAN  

    In developed countries including Japan, malignant tumor (cancer), heart disease and cerebral apoplexy are major causes of death, but infectious diseases are still responsible for a high number of deaths in developing countries, especially among children aged less than 5 years. World Health Statistics published by WHO reports a high percentage of mortality from infectious diseases in children, and many of these diseases may be subject to transmission across borders and could possibly invade Japan.
    Given this situation, the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan initiated Phase I of the Program of Founding Research Centers for Emerging and Reemerging Infectious Disease, which ran from FY 2005 to 2009, and involved 8 Japanese universities and 2 research centers. The program was established for the following purposes: 1) creation of a domestic research structure to promote the accumulation of fundamental knowledge about infectious diseases, 2) establishment of 13 overseas research collaboration centers in 8 countries at high risk of emerging and reemerging infections and at which Japanese researchers are stationed and conduct research in partnership with overseas instructors, 3) development of a network among domestic and overseas research centers, and 4) development of human resources.
    The program was controlled under MEXT and managed by the RIKEN Center of Research Network for Infectious Diseases (Riken CRNID). Phase II of the program was set up as the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID), and has been running in FY 2010-2014.
    Phase III will start in April 2015, and will be organized by the newly established Japanese governmental organization "Japan Agency for Medical Research and Development (AMED)'', the so-called Japanese style NIH.
    The Collaborative Research Center of Okayama University for Infectious Diseases in India (CRCOUI) was started up in 2007 at the National Institute of Cholera and Enteric Disease, Kolkata, India. Major projects of CRCOUI are concerned with diarrheal diseases such as, 1) active surveillance of diarrhea! patients, 2) development of dysentery vaccines, 3) viable but nonculturable (VBNC) Vibrio cholerae, and 4) pathogenic mechanisms of various diarrhogenic microorganisms.
    This review article outlines project of J-GRID and CRCOUI which the authors carried out collaboratively with NICED staff members.

    DOI: 10.4265/bio.20.77

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  • Stepwise changes in viable but nonculturable Vibrio cholerae cells

    Daisuke Imamura, Tamaki Mizuno, Shin-ichi Miyoshi, Sumio Shinoda

    MICROBIOLOGY AND IMMUNOLOGY   59 ( 5 )   305 - 310   2015年5月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.

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  • インドにおける感染症研究の連携:岡山大学インド感染症共同研究センターとコレラおよび腸管感染症研究所 (NICED)

    篠田 純男, 今村 大輔, 水野 環, 三好 伸一

    最新医学   70 ( 4 )   738 - 744   2015年

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  • Defensive Effects of Human Intestinal Antimicrobial Peptides against Infectious Diseases Caused by Vibrio mimicus and V. vulnificus

    Shin-Ichi Miyoshi, Hiroto Ikehara, Mika Kumagai, Tamaki Mizuno, Tomoka Kawase, Yoko Maehara

    BIOCONTROL SCIENCE   19 ( 4 )   199 - 203   2014年12月

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    記述言語:英語   出版者・発行元:SOC ANTIBACTERIAL & ANTIFUNGAL AGENTS, JAPAN  

    Of human pathogenic Vibrio species, V mimicus causes gastroenteritis whereas V vulnificus causes fatal septicemia after consumption of contaminated seafood. These two pathogens produce hemolytic toxins termed V mimicus hemolysin (VMH) and V vulnificus hemolysin (VVH), respectively. These toxins elicit the cytolysis of various eukaryotic cells, as well as erythrocytes. The human intestine secretes cationic antimicrobial peptides (AMPs) to prevent infectious diseases. Paneth cells in the small intestine secrete a-defensin 5 (HD-5) and epithelial cells in the large intestine produce LL-37. In the present study, we examined the bactericidal activities of AMPs against V mimicus and V vulnificus. Although HD-5 showed no bactericidal activity, LL-37 revealed significant activity against both Vibrio species, suggesting that neither V mimicus nor V vulnificus can multiply in the large intestine. We also tested whether AMPs had the ability to inactivate the hemolytic toxins. Only HD-5 was found to inactivate VMH, but not VVH, in a dose-dependent manner through the direct binding to VMH. Therefore, it is considered that V mimicus cannot penetrate the small intestinal epithelium because the cytolytic action of VMH is inactivated by HD-5.

    DOI: 10.4265/bio.19.199

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  • A novel extracellular protease of Vibrio mimicus that mediates maturation of an endogenous hemolysin

    Tamaki Mizuno, Ayako Nanko, Yoko Maehara, Sumio Shinoda, Shin-Ichi Miyoshi

    MICROBIOLOGY AND IMMUNOLOGY   58 ( 9 )   503 - 512   2014年9月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Vibrio mimicus, a human pathogen that causes gastroenteritis, produces an enterotoxic hemolysin as a virulence factor. The hemolysin is secreted extracellularly as an inactive protoxin and converted to a mature toxin through removal of the N-terminal propeptide, which comprises 151 amino acid residues. In this study, a novel protease having the trypsin-like substrate specificity was purified from the bacterial culture supernatant. The N-terminal amino acid sequence of the purified protein was identical with putative trypsin VMD27150 of V. mimicus strain VM573. The purified protease was found to cause maturation of the protoxin by cleavage of the Arg(151)Ser(152) bond. Deletion of the protease gene resulted in increased amounts of the protoxin in the culture supernatant. In addition, expression of the hemolysin and protease genes was detected during the logarithmic growth phase. These findings indicate that the protease purified may mediate maturation of the hemolysin.

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  • Evaluation of real-time loop-mediated isothermal amplification (RealAmp) for rapid detection of Mycobacterium tuberculosis from sputum samples

    Yiming Li, Lei Shi, Anqi Pan, Weiwei Cao, Xun Chen, Hecheng Meng, He Yan, Shin-ichi Miyoshi, Lei Ye

    JOURNAL OF MICROBIOLOGICAL METHODS   104   55 - 58   2014年9月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) leads to serious health problems as a chronic respiratory infectious disease. Here we established a real-time fluorescence loop-mediated isothermal amplification assay (RealAmp) using a portable ESE Quant tube scanner as a convenient rapid detection method for MTB. The method efficacy from sputum samples was further investigated, and the reaction time was only 20 min with the detection limit low to 10(2) CPU/ml concentration of MTB. We assessed a total of 1067 samples by the RealAmp assay, comparing the results with smear microscopy and conventional culture methods. To examine whether the failure to detect TB by culturing is due to low sensitivity or true absence, we examined the culture negative samples by commercial real time PCR MTB detection kit, and the results were compared with RealAmp. The data showed that RealAmp assay had a higher positive rate than that of sputum smear and culture methods. RealAmp had a sensitivity of 96.70% and a specificity of 91.55% when compared with culture. In addition, its sensitivity and specificity were 95.29% and 86.88% respectively compared with examination of smear samples using light microscopy. The sensitivity of RealAmp in comparison to real time PCR was 98.25% and specificity was 99.11% in validation of culture negative samples. The present study revealed the newly established RealAmp assay as a convenient, efficient, sensitive and specific method that could be an alternative for rapid detection of MTB and a tool to validate culture and smear negative samples. Furthermore, the portability of the ESE Quant tube scanner also contributed to the promising application for grassroots and field detection of MTB. (C) 2014 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.mimet.2014.06.011

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  • Isolation of viable but nonculturable Vibrio cholerae O1 from environmental water samples in Kolkata, India, in a culturable state

    Mitsutoshi Senoh, Jayeeta Ghosh-Banerjee, Tamaki Mizuno, Sumio Shinoda, Shin-ichi Miyoshi, Takashi Hamabata, G. Balakrish Nair, Yoshifumi Takeda

    MICROBIOLOGYOPEN   3 ( 2 )   239 - 246   2014年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Previously, we reported that viable but nonculturable (VBNC) Vibrio cholerae was converted into a culturable state by coculture with several eukaryotic cell lines including HT-29 cells. In this study, we found that a factor converting VBNC V. cholerae into a culturable state (FCVC) existed in cell extracts of eukaryotic cells. FCVC was nondialyzable, proteinase K-sensitive, and stable to heating at <60 degrees C for 5 min. We prepared thiosulfate citrate bile salts sucrose (TCBS) plates with FCVC (F-TCBS plates). After confirming that VBNC V. cholerae O1 and O139 formed typical yellow colonies on F-TCBS plates, we tried to isolate cholera toxin gene-positive VBNC V. cholerae from environmental water samples collected in urban slum areas of Kolkata, India and succeeded in isolating V. cholerae O1 El Tor variant strains harboring a gene for the cholera toxin. The possible importance of VBNC V. cholerae O1 as a source of cholera outbreaks is discussed.

    DOI: 10.1002/mbo3.164

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  • Effects of temperature, growth phase and luxO-disruption on regulation systems of toxin production in Vibrio vulnificus strain L-180, a human clinical isolate

    Abdelaziz Elgaml, Kazutaka Higaki, Shin-ichi Miyoshi

    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY   30 ( 2 )   681 - 691   2014年2月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Vibrio vulnificus is a halophilic estuarine bacterium while it causes fatal septicemia or necrotizing wound infections in humans. This pathogen secretes the metalloprotease (V. vulnificus protease: VVP) and the cytolysin (V. vulnificus hemolysin: VVH) as protein toxins; however, their production was coordinated in response to the bacterial cell density. This regulation is termed quorum sensing (QS) and is mediated by the small diffusible molecule called autoinducer 2 (AI-2). In the present study, we investigated effects of disruption of luxO encoding a central response regulator of the QS circuit, as well as effects of temperature and growth phase, on the toxin production by V. vulnificus. Disruption of luxO was found to increase VVP production and expression of its gene vvpE. The expression of smcR, crp and rpoS, of which products positively regulate vvpE expression, and luxS encoding the AI-2 synthetase were also significantly increased. On the other hand, the luxO disruption resulted in reduction of VVH production and expression of its gene vvhA. Expression of other two genes affecting the QS circuit, luxT and rpoN, were also significantly decreased. The regulation systems of VVP production were found to exert their action during the stationary phase of the bacterial growth and to be operated strongly at 26 A degrees C. By contrast, those of VVH production apparently started at the log phase and were operated more effectively at 37 A degrees C.

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  • Activity of Collaborative Research Center of Okayama University for Infectious Disease in India

    Shinoda S, Imamura D, Mizuno T, Miyoshi S

    J Disast Res   9 ( 5 )   774 - 783   2014年

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  • Regulation system of serine protease production in Vibrio vulnificus strain NCIMB 2137, a metalloprotease-gene negative strain isolated from a diseased eel

    Abdelaziz Elgaml, Kazutaka Higaki, Shin-ichi Miyoshi

    AQUACULTURE   416   315 - 321   2013年12月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Vibrio vulnificus is a ubiquitous estuarine microorganism but causes fatal systemic infections in cultured eels or shrimps, as well as in immunocompromised humans. An extracellular metalloprotease has been reported to be a potential virulence factor of the bacterium; however, a few strains isolated from a diseased eel or shrimp were recently found to produce a serine protease termed VvsA. In the present study, we first clarified the regulatory characteristics of the VvsA production in V. vulnificus strain NCIMB 2137, a metalloprotease-gene negative strain isolated from a diseased eel. V. vulnificus coordinates expression of virulence genes in response to the bacterial cell density, which is termed quorum sensing (QS) and is mediated by the small diffusible molecule called autoinducer 2 (AI-2). When cultivated at 26 degrees C, the vvsA expression was closely related with expression of the luxS gene encoding the synthase of the AI-2 precursor LuxS. Both VvsA and AI-2 were sufficiently secreted at early stationary phase of the bacterial growth. In contrast, when cultivated at 37 degrees C, far less amounts of the AI-2 and VvsA were produced even at the stationary phase. Disruption of the luxS gene was found to decrease significantly the vvsA expression and VvsA production. Disruption of luxO encoding the central response regulator of the QS circuit caused an increase in the vvsA expression and VvsA production at the logarithmic growth phase. These findings indicate that VvsA production is positively regulated by the V. vulnificus LuxS-dependent QS system, which operated more effectively at 26 degrees C than at 37 degrees C. (C) 2013 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.aquaculture.2013.09.041

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  • Ecological Study of Pathogenic Vibrios in Aquatic Environments

    Sumio Shinoda, Yuki Furumai, Sei-Ichi Katayama, Tamaki Mizuno, Shin-Ichi Miyoshi

    BIOCONTROL SCIENCE   18 ( 1 )   53 - 58   2013年3月

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    記述言語:英語   出版者・発行元:SOC ANTIBACTERIAL & ANTIFUNGAL AGENTS, JAPAN  

    An ecological study of pathogenic vibrios in aquatic environments of Okayama was carried out. The number of Vibrio parahaemolyticus detected in the sea area was comparatively smaler than that found in the survey of about two decades ago. Various reasons for the decrease in the case of food poisoning by V. parahaemolyticus have been suggested but the lower number of the vibrio in aquatic environments may be one explanation. Although the number of V. vulnificus was also not as large, most of the isolates possessed the pathogenic genes, vvp and vvh, suggesting the potential for fatal pathogenicity to patients having underlying diseases. As for V. cholerae, some non-O1/non-O139 serovar isolates were detected in a fresh water area, and many of them had hlyA, the gene for hemolysin which acts as a pathogenic factor in sporadic cases of diarrhea. Thus, the total number of pathogenic vibrios detected was not of concern. However, the marine products of these areas are shipped in wide area and are for general consumption. Therefore, it is necessary to continue to survey pathogenic vibrios in aquatic environments in order to ensure food hygiene.

    DOI: 10.4265/bio.18.53

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  • Extracellular proteolytic enzymes produced by human pathogenic Vibrio species

    Shinoda S, Miyoshi S

    Frontiers in Microbiology   4   1 - 8   2013年

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  • ビブリオ・バルニフィカス感染

    三好伸一

    化学療法の領域   29 ( 7 )   1454 - 1459   2013年

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  • Conversion of viable but nonculturable enteric bacteria to culturable by co-culture with eukaryotic cells

    Mitsutoshi Senoh, Jayeeta Ghosh-Banerjee, Thandavarayan Ramamurthy, Rita R. Colwell, Shin-ichi Miyoshi, G. Balakrish Nair, Yoshifumi Takeda

    MICROBIOLOGY AND IMMUNOLOGY   56 ( 5 )   342 - 345   2012年5月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Viable but nonculturable (VBNC) Vibrio cholerae non-O1/non-O139, V. parahaemolyticus, enterohemorrhagic Escherichia coli, enterotoxigenic E. coli, enteropathogenic E. coli, Shigella flexneri, and Salmonella enterica were converted to the culturable state by co-culture with selected eukaryotic cells, e.g., HT-29, Caco-2, T84, HeLa, Intestine 407, and CHO cells.

    DOI: 10.1111/j.1348-0421.2012.00440.x

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  • Possible variation of the human oral bacterial community after wearing removable partial dentures by DGGE

    Xiao Zhu, Shaohai Wang, Yihai Gu, Xiaoyu Li, Hui Yan, He Yan, Shin-ichi Miyoshi, Lei Shi

    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY   28 ( 5 )   2229 - 2236   2012年5月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Although it is well-known that variations of the microbial community in a specific location of human body may be associated with some diseases, the developing change of the oral microbiota related to oral diseases before and after wearing the removable partial dentures (RPD) is not completely understood. In this study, three kinds of samples (saliva, supra- and subgingival plaque, and oral mucosal surfaces) were collected from the 10-patients group at three different times: before, 1-month and 6-months after the treatment. Ten healthy adults were also selected as the control group. Denaturing gradient gel electrophoresis was applied to identify the bacterial profiles and to analyze the dynamics of the oral microbial population in the pre- and post-therapy. The ANOVA of Repeated Measurement Data indicated that, in the saliva and mucosal surfaces, wearing RPDs caused significant change of numbers of amplicons. As many as 607 amplicons were chosen to cut out and re-amplify by PCR. After cloning and sequencing, a total of 16 bacterial genera were identified. The health-associated genera such as Streptococcus, Neisseria, Rothia, Corynebacterium, Leptotrichia, Gemella, Veillonella, Selenomona and Actinomyces tended to decrease, whereas the disease-associated species including Streptococcus mutans tended to increase. In general, wearing RPDs influenced the diversity of the bacterial species in the oral microbial ecosystem. It is noteworthy that the oral environment will be changed from the healthy status towards the disease status after the treatment.

    DOI: 10.1007/s11274-012-1030-5

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  • An extracellular serine protease produced by Vibrio vulnificus NCIMB 2137, a metalloprotease-gene negative strain isolated from a diseased eel

    Shin-ichi Miyoshi, Jiyou Wang, Keizo Katoh, Mitsutoshi Senoh, Tamaki Mizuno, Yoko Maehara

    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY   28 ( 4 )   1633 - 1639   2012年4月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Vibrio vulnificus is a ubiquitous estuarine microorganism but causes fatal systemic infections in immunocompromised humans, cultured eels or shrimps. An extracellular metalloprotease VVP/VvpE has been reported to be a potential virulence factor of the bacterium; however, a few strains isolated from a diseased eel or shrimp were recently found to produce a serine protease termed VvsA, but not VVP/VvpE. In the present study, we found that these strains had lost the 80 kb genomic region including the gene encoding VVP/VvpE. We also purified VvsA from the culture supernatant through ammonium sulfate fractionation, gel filtration and ion-exchange column chromatography, and the enzyme was demonstrated to be a chymotrypsin-like protease, as well as those from some vibrios. The gene vvsA was shown to constitute an operon with a downstream gene vvsB, and several Vibrio species were found to have orthologues of vvsAB. These findings indicate that the genes vvp/vvpE and vvsAB might be mobile genetic elements.

    DOI: 10.1007/s11274-011-0969-y

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  • Development of a sensitive rRNA-targeted reverse transcription-quantitative polymerase chain reaction for detection of Vibrio cholerae/mimicus, V. parahaemolyticus/alginolyticus and Campylobacter jejuni/coli

    Takashi Kurakawa, Hiroyuki Kubota, Hirokazu Tsuji, Kazunori Matsuda, Takashi Asahara, Takuya Takahashi, Thandavarayan Ramamurthy, Takashi Hamabata, Eizo Takahashi, Shin-ichi Miyoshi, Keinosuke Okamoto, Asish K. Mukhopadhyay, Yoshifumi Takeda, Koji Nomoto

    MICROBIOLOGY AND IMMUNOLOGY   56 ( 1 )   10 - 20   2012年1月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    A sensitive rRNA-targeted reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method was developed for detection of Vibrio cholerae/mimicus, V. parahaemolyticus/alginolyticus and Campylobacter jejuni/coli by using specific primers. Counts of the enteric pathogens spiked in human stools were quantified at the lower detection limit of 103 cells/g stool by RT-qPCR, in marked contrast with conventional quantitative polymerase chain reaction (qPCR) at the detection limit of 105 to 106 cells/g stool. The bacterial counts determined by RT-qPCR were almost equivalent to those determined by the culture method and fluorescence in situ hybridization (FISH) during the course of in vitro culture. Bacterial rRNA in the stools was stable for at least 4 weeks when the stools were kept as the suspensions in RNA-stabilizing agent, RNAlater (R), even at 37oC. These data suggested that the rapid and high sensitive rRNA-targeted RT-qPCR was applicable for the accurate quantification of viable enteric pathogens, such as V. cholerae/mimicus, V. parahaemolyticus/alginolyticus and C. jejuni/coli.

    DOI: 10.1111/j.1348-0421.2011.00405.x

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  • Characterization and horizontal transfer of class 1 integrons in Salmonella strains isolated from food products of animal origin

    Hecheng Meng, Zhigang Zhang, Miaorui Chen, Yongyu Su, Lin Li, Shin-ichi Miyoshi, He Yan, Lei Shi

    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY   149 ( 3 )   274 - 277   2011年10月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    A total of 81 Salmonella isolates from retail meats and seafood in Hebei province, China, were assayed for the presence of and horizontal transfer of class 1 integrons. By the PCR screening for the integrons, class 1 integron was detected from strains in serotypes of Derby, Indiana, London and Choleraesuis, which were isolated from pork, chicken or seafood: however, two isolates contained the empty integron that lacked the resistance cassette, a potential hotspot for development of the multidrug resistance. In contrast, two other isolates had the antibiotic resistance gene cassettes within the class 1 integron, which were dfrA1-aadA1 and aadB-cmlA, respectively. The conjugation experiments demonstrated the plasmid-mediated transfer of the class 1 integrons. Furthermore, each of the integrons was transmitted to Streptococcus mutans via natural gene transformation. These findings suggest the possible transfer of class 1 integrons from foodborne pathogens to human residential bacteria via horizontal gene transfer. (C) 2011 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.ijfoodmicro.2011.07.006

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  • Inactivation of Vibrio vulnificus hemolysin through mutation of the N- or C-terminus of the lectin-like domain

    Shin-ichi Miyoshi, Yuki Abe, Mitsutoshi Senoh, Tamaki Mizuno, Yoko Maehara, Hiroshi Nakao

    TOXICON   57 ( 6 )   904 - 908   2011年5月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Vibrio vulnificus is an etiological agent causing serious systemic infections in the immu-nocompromised humans or cultured eels. This species commonly produces a hemolytic toxin consisting of the cytolysin domain and the lectin-like domain. For hemolysis, the lectin-like domain specifically binds to cholesterol in the erythrocyte membrane, and to form a hollow oligomer, the toxin is subsequently assembled on the membrane. The cytolysin domain is essential for the process to form the oligomer. Three-dimensional structure model revealed that two domains connected linearly and the C-terminus was located near to the joint of the domains. Insertion of amino acid residues between two domains was found to cause inactivation of the toxin. In the C-terminus, deletion, substitution or addition of an amino acid residue also elicited reduction of the activity. However, the cholesterol-binding ability was not affected by the mutations. These results suggest that mutation of the C- or N-terminus of the lectin-like domain may result in blockage of the toxin assembly. (C) 2011 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.toxicon.2011.03.013

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  • Proteases Produced by Vibrios

    Sumio Shinoda, Shin-Ichi Miyoshi

    BIOCONTROL SCIENCE   16 ( 1 )   1 - 11   2011年3月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:SOC ANTIBACTERIAL & ANTIFUNGAL AGENTS, JAPAN  

    Bacteria of the genus Vibrio are normal habitants of the aquatic environment but the some species are believed to be human pathogens. Pathogenic vibrios produce various pathogenic factors, and the proteases are also recognized to play pathogenic roles in the infection: the direct: roles by digesting many kinds of host proteins or indirect roles by processing other pathogenic protein factors. Especially VVP from Vibrio vulnificus is thought to be a major pathogenic factor of the vibrio. Although HA/P, the V. cholerae hemagglutinin/protease, is not a direct toxic factor of cholera vibrio, its significance is an undeniable fact. Production of HA/P is regulated together with major pathogenic factors such as CT (cholera toxin) or TCP (toxin co-regulated pilus) by a quorum-sensing system. HA/P is necessary for full expression of pathogenicity of the vibrio by supporting growth and translocation in the digestive tract. Processing of protein toxins such as CT or El Tor hemolysin is also an important pathogenic role.

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  • Prevalence and antimicrobial resistance of Salmonella in retail foods in northern China

    He Yan, Lin Li, M. Jahangir Alam, Sumio Shinoda, Shin-ichi Miyoshi, Lei Shi

    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY   143 ( 3 )   230 - 234   2010年10月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    A total of 387 retail meat, seafood and milk powder samples were collected from nine cities in northern China in 2005 and screened for the presence of Salmonella. Salmonella strains isolated were subjected to serotyping and antimicrobial susceptibility testing. Salmonella was isolated from 81 (20.9%, 81/387) samples and classified into 23 serotypes. The isolates were frequently resistant to sulfamethoxazole (86.4%), sulfamethoxazole/trimethoprim (48.1%), nalidixic acid (30.9%). tetracycline (19.8%), carboxybenzylpenicillin (17.3%), amoxicillin (17.3%) and ampicillin (16.0%). The multiple resistance (resistance to >= 3 antibiotics) was found in 29.6% (n = 24) isolates. Additionally, 4 isolates from chicken displayed the ACSSuTNx profile, resistant to ampicillin, chloramphenicol, streptomycin, sulfonamide, tetracycline and nalidixic acid, in particular, strain HBS084 showing the resistance to as many as 20 antibiotics. Salmonella from chicken showed the higher frequency of antimicrobial resistance. Our findings indicate that in northern China food products of animal origin can be a source of exposure for consumers to multiresistant Salmonella strains. (c) 2010 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.ijfoodmicro.2010.07.034

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  • Defensive Effects of Human Antimicrobial Peptide alpha-Defensins against Enterococcus faecalis

    Shin-ichi Miyoshi, Kenta Koyama, Tamaki Mizuno, Minoru Kashihara, Yoko Maehara, Hiroshi Nakao

    JOURNAL OF HEALTH SCIENCE   56 ( 5 )   618 - 622   2010年10月

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    記述言語:英語   出版者・発行元:PHARMACEUTICAL SOC JAPAN  

    Cationic and amphiphilic antimicrobial peptides (AMPs) such as alpha-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and alpha-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The alpha-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither alpha-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the alpha-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that alpha-defensins may be defensive factors against E. faecalis in humans.

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  • Assimilation of Metal Ions Bound to Porphyrins or Porphyrin-Peptides by Vibrio vulnificus, a Human Pathogen Inhabiting Estuarine and Marine Environments

    Shin-Ichi Miyoshi, Tomoko Sasaki, Nahoko Kaku, Takaharu Inoue, Natsuki Uozumi, Yoko Maehara, Hiroshi Nakao

    BIOCONTROL SCIENCE   15 ( 1 )   1 - 6   2010年3月

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    記述言語:英語   出版者・発行元:SOC ANTIBACTERIAL & ANTIFUNGAL AGENTS, JAPAN  

    Vibrio vulnificus, a ubiquitous microorganism in aquatic environments, causes serious septicemia to the immunocompromised host. In addition to protoheme, this species can utilize Fe-TCPP [ferric tetrakis (4-carboxyphenyl) porphine] as an iron source. In the present study, heme c bound covalently to the protein in cytochrome c, as well as the Fe-TCPP complex formed with a nanopeptide with a high affinity, was found to be useful iron sources for V. vulnificus. This bacterium was also revealed to use Zn-TCPP as a single zinc source. However, other metalloporphyrins such as Mn-TCPP and Pt-TCPP delayed the bacterial growth in the broth containing Fe-TCPP, suggesting interference in the iron assimilation. These results indicate that V. vulnificus may acquire metal ions from both free and peptide-bound metalloporphyrins.

    DOI: 10.4265/bio.15.1

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  • Modulation of Vibrio mimicus hemolysin through limited proteolysis by an endogenous metalloprotease

    Tamaki Mizuno, Syed Z. Sultan, Yoshimi Kaneko, Tomonaga Yoshimura, Yoko Maehara, Hiroshi Nakao, Tomofusa Tsuchiya, Sumio Shinoda, Shin-ichi Miyoshi

    FEBS JOURNAL   276 ( 3 )   825 - 834   2009年2月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Vibrio mimicus is a causative agent of human gastroenteritis and food poisoning, and this species produces an enterotoxic hemolysin (V. mimicus hemolysin) as a virulence determinant. Vibrio mimicus hemolysin is secreted as an 80 kDa precursor, which is later converted to the 66 kDa mature toxin through removal of an N-terminal propeptide via cleavage of the Arg151-Ser152 bond. In this article, we investigate the role of the endogenous metalloprotease (V. mimicus protease) in the maturation of V. mimicus hemolysin. In vitro experiments using purified proteins showed that, although it activated the precursor at the early stage via cleavage of the Asn157-Val158 bond, V. mimicus protease finally converted the activated and physiologically maturated toxin to a 51 kDa protein through removal of the C-terminal polypeptide. This 51 kDa derivative was unable to lyse erythrocytes because of its inability to bind to the erythrocyte membrane. Vibrio mimicus protease-negative strains were found to produce high levels of V. mimicus hemolysin at the logarithmic phase of bacterial growth and maintained high hemolytic activity even at the stationary phase. These findings indicate that, although it is not directly related to toxin maturation in vivo, V. mimicus protease can modulate the activity of V. mimicus hemolysin and/or its precursor through limited proteolysis.

    DOI: 10.1111/j.1742-4658.2008.06827.x

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  • Specific detection of viable Salmonella cells by an ethidium monoazide-loop mediated isothermal amplification (EMA-LAMP) method

    Lu Y, Yang W, Shi L, Li I, Alam MJ, Guo S, Miyoshi S

    Journal of Health Science   54 ( 6 )   686 - 691   2009年

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  • Role of the Enterotoxic Hemolysin in Pathogenicity of Vibrio mimicus

    Tho Li, Akiko Kobayashi, Noriko Takata, Tomonaga Yoshimura, Yoko Maehara, Tomofusa Tsuchiya, Shin-ichi Miyoshi

    JOURNAL OF HEALTH SCIENCE   54 ( 6 )   686 - 691   2008年12月

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    記述言語:英語   出版者・発行元:PHARMACEUTICAL SOC JAPAN  

    Vibrio mimicus (V mimicus) is a causative agent of human gastroenteritis and food poisoning. Although several toxic or virulence factors have been isolated from the bacterium, an enterotoxic hemolysin is a sole toxin produced by all clinical isolates. In the present study, we found that the antibody against the hemolysin significantly inhibited the fluid-accumulating action of the living cells inoculated into a rabbit ileal loop, and that the hemolysin gene (vmhA) was probably expressed by the bacterium in the ileal loop. Additionally, in spit of the comparable motility and similar proteome profiles, a vmhA mutant revealed the reduced fluid-accumulating activity. Theses findings suggest that the hemolysin contributes to full virulence of V. mimicus.

    DOI: 10.1248/jhs.54.686

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  • Differential gene expression and extracellular secretion of the collagenolytic enzymes by the pathogen Vibrio parahaemolyticus

    Shin-ichi Miyoshi, Yuko Nitanda, Kaori Fujii, Kiyomi Kawahara, Tao Li, Yoko Maehara, Thandavarayan Ramamurthy, Yoshifumi Takeda, Sumio Shinoda

    FEMS MICROBIOLOGY LETTERS   283 ( 2 )   176 - 181   2008年6月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Vibrio parahaemolyticus, a causative agent of wound infections as well as food poisoning, harbors two collagenase genes: vppC and prtV. When cultivated at 26 degrees C in gelatin broth supplemented with 3.0% NaCl, significant collagenolytic activity was detected in the culture supernatant at the early stationary phase. Native polyacrylamide gel electrophoresis analysis revealed a 90-kDa protein, and N-terminal amino acid sequencing showed that this protein was VppC, generated through truncation of 72 N-terminal amino acid residues. Additionally, significant expression of only vppC was observed by reverse transcriptase PCR. By contrast, a vppC-negative mutant constructed through single crossover homologous recombination secreted a 50-kDa-collagenolytic enzyme; however, this enzyme was a serine protease that was reported previously. These results suggest that VppC is a primary extracellular collagenase produced by V. parahaemolyticus.

    DOI: 10.1111/j.1574-6968.2008.01159.x

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  • Variation of extracellular proteases produced by Vibrio vulnificus clinical isolates: Genetic diversity of the metalloprotease gene (vvp), and serine protease secretion by vvp-negative strains

    Jiyou Wang, Tomoko Sasaki, Yoko Maehara, Hiroshi Nakao, Tomofusa Tsuchiya, Shin-ichi Miyoshi

    MICROBIAL PATHOGENESIS   44 ( 6 )   494 - 500   2008年6月

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    記述言語:英語   出版者・発行元:ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD  

    Vibrio vulnificus is a causative agent of septicemia or wound infection in human and eel; however. the genetic variation between human and eel isolates has been reported. In the present study, the difference in the vvp gene encoding a tissue-damaging metalloprotease Was (type B vvp) was 95.2% identical with that of strain L-180 from human blood investigated. The acne of strain E86 from a diseased eel (type A vvp). PCR using oligonucleotide primers designed to differentiate two types of the acne showed that eel avirulent strains (9 isolates) commonly carry type A ut;p. whereas eel virulent strains (18 isolates) revealed significant genetic variation. The vvp genes From strains including strain E86 were placed on type B while those from 3 strains, were on type A. other strains were found to he negative but analysis Showed that they secreted a serine protease (VVA0302) instead of the negative, but PAGE and amino acid sequencing metalloprotease. This protease is all orthologue of a toxic protease from Vibrio parahaemolyticus a human pathogen causing wound infection as well Lis gastroenteritis. These findings suggest that, in addition to metalloprotease. the extracellular serine protease may contribute to pathogenicity of V. vulnificus. (C) 2008 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.micpath.2008.01.001

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  • Molecular epidemiological studies of Vibrio cholerae in Bengal region

    Sumio Shinoda, Tomoko Nakagawa, Nobuyuki Hirakawa, Shin-Ichi Miyoshi, Eiji Arakawa, Thandavarayan Ramamurthy, B. Dutta, Shah M. Faruque, Gopinath Balakrish Nair

    BIOCONTROL SCIENCE   13 ( 1 )   1 - 8   2008年3月

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    記述言語:英語   出版者・発行元:SOC ANTIBACTERIAL & ANTIFUNGAL AGENTS, JAPAN  

    Vibrio cholerae isolates from environmental and clinical origins in the Bengal region in which epidemics of cholera break out periodically were analyzed with particular emphasis on the molecular epidemiological features. The presence of the virulence genes (ctxA, tcpA and toxR) in the isolates was analyzed by the PCR (polymerase chain reaction) method. PFGE (pulsed-field gel electrophoresis) was performed to determine the clonal relationships between the clinical and environmental strains. Antiblograms and O serovars of the isolates were also examined. O1 and O139 strains from both clinical and environmental sources were all positive for the three virulence genes while non-O1/non-O139 strains from both sources were all negative for ctxA and tcpA but positive for toxR. PFGE patterns of recent isolates of O1 and O139 were similar in each serovar regardless of origin, suggesting a clonal relationship between the clinical and environmental strains, although comparison with past isolates or isolates from different geographical area showed some differences.

    DOI: 10.4265/bio.13.1

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  • The crucial amino acid residue related to inactivation of Vibrio vulnificus hemolysin

    Mitsutoshi Senoh, Yuka Okita, Sumio Shinoda, Shin-ichi Miyoshi

    MICROBIAL PATHOGENESIS   44 ( 1 )   78 - 83   2008年1月

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    記述言語:英語   出版者・発行元:ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD  

    Vibrio vulnificus. all opportunistic human pathogen causing fetal septicemia, produces a 50-kDa pore-forming toxin as a virulence factor. This toxin consists of 451 amino acid residues: however. there are two types of this toxin on the basis of the difference of some,amino acid residues, type 1 (Leu(281), Ser(415), Asn(435)/Asp(435), Asn(438)) and type 2 (Ile(281), Asn(415), Asn(435), Thr(438)). In the present study, two characteristic properties of type 2 toxin that was elaborated by V. vulnificus cells or synthesized by the in vitro system were compared to those of type 1 toxin. Type 2 toxin was found to be more resistant to spontaneous inactivation at 37 degrees C and to specific inactivation by cholesterol. On the other hand a variant of type 2 toxin (Asp(435), Asn(438)) showed the same properties as type 1 toxin. The replacement of the 438th Asn to Thr (N438T), but not the 435th Asp to Asn (D435N). resulted in reversion of the variant type 2 toxin to typical type 2 toxin. These findings indicate that a single amino acid residue. Thr(438), may be critical for higher stability of type 2 toxin. (C) 2007 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.micpath.2007.07.002

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  • ビブリオ・バルニフィカスの病原性

    三好伸一

    化学療法の領域   24 ( 6 )   879 - 884   2008年

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  • A plasmidic class 1 integron from five Pseudomonas aeruginosa clinical strains harbored aacA4 and nonsense-mutated cmIA1 gene cassettes

    He Yan, Lei Shi, Shinji Yamasaki, Xinhui Li, Yicheng Cao, Lin Li, Liansheng Yanga, Shin-ichi Miyoshi

    JOURNAL OF HEALTH SCIENCE   53 ( 6 )   750 - 755   2007年12月

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    記述言語:英語   出版者・発行元:PHARMACEUTICAL SOC JAPAN  

    Five strains of multidrug-resistant Pseudomonas aeruginosa (P aeruginosa) were isolated from inpatients at a local hospital in China. The most frequent resistance was to cefoperazone, ciprofloxacin, ceftriaxone, cefotaxime, gentamicin, piperacillin, trimethoprim sulfamethoxazole, and tobramycin. These strains were found to contain the class 1 integron, in which the 2360-bp gene cassettes were flanked by 5'- and 3'-conserved segments. Sequence analysis revealed that the gene cassettes contained aacA4 and cmlA1 genes; however, the latter gene had a nonsense mutation resulting in the production of a truncated protein. To the best of our knowledge, this is the first report of a nonsense mutation in the cmlA1 gene. Moreover, the R aeruginosa strains showed identical profiles in pulsed-field gel electrophoresis, suggesting that they were derived from the same clone. These results emphasize the importance of controlling the spread of multidrug-resistant pathogens in hospitals.

    DOI: 10.1248/jhs.53.750

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  • Growth phase dependant activation of the precursor of Vibrio mimicus hemolysin (Pro-VMH)

    Zafar Sultan, Thmaki Mizuno, Aki Sakurai, Noriko Takata, Keinosuke Okamoto, Shin-ichi Miyoshi

    JOURNAL OF HEALTH SCIENCE   53 ( 4 )   430 - 434   2007年8月

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    記述言語:英語   出版者・発行元:PHARMACEUTICAL SOC JAPAN  

    Vibrio mimicus (V mimicus), a causative agent of gastroenteritis and food poisoning, secretes a 63-kDa enterotoxic hemolysin as the most potent virulence factor. The vmhA gene encoding an 83-kDa precursor of the hemolysin was expressed from the early to late log phase of the bacterial growth, and the 79-kDa inactive protoxin was detected from the culture supernatant in the same growth phase. The N-terminal amino acid sequence of the protoxin was determined to be NH2-Asn-Ile-Ser-Asp-Pro-Val indicating cleavage of the Ala(25)-Asn(26) bond by a signal peptidase. In contrast, the hemolytic activity and the mature hemolysin in the culture supernatant were detected only at the late log phase. The maturation of the hemolysin, therefore, is suggested to be achieved by two-step processing, cleavage of the signal peptide followed by the growth phase-dependent removal of the 16-kDa propeptide.

    DOI: 10.1248/jhs.53.430

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  • Haemolysin produced by Vibrio mimicus activates two Cl- secretory pathways in cultured intestinal-like Caco-2 cells

    Akira Takahashi, Shin-ichi Miyoshi, Noriko Takata, Masayuki Nakano, Akiko Hamamoto, Kazuaki Mawatari, Nagakatsu Harada, Sumio Shinoda, Yutaka Nakaya

    CELLULAR MICROBIOLOGY   9 ( 3 )   583 - 595   2007年3月

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    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING  

    Haemolysin (VMH) is a virulent factor produced by Vibrio mimicus, a human pathogen that causes diarrhoea. As intestinal epithelial cells are the primary targets of haemolysin, we investigated its effects on ion transport in human colonic epithelial Caco-2 cells. VMH increased the cellular short circuit current (Isc), used to estimated ion fluxes, and I-125 efflux of the cells. The VMH-induced increases in Isc and I-125 efflux were suppressed by depleting Ca2+ from the medium or by pretreating the cells with BAPTA-AM or by Rp-adenosin 3',5'-cyclic monophosphorothioate triethylammonium salt (Rp-cAMPS). The Cl- channel inhibitors 4,4'-disothiocyanatostibene-2,2'-disulfonic acid (DIDS), glybenclamide, and 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) suppressed the VMH-induced increases in Isc and I-125 efflux. Moreover, VMH increased the intracellular concentrations of Ca2+ and cAMP. Thus, VMH stimulates Caco-2 cells to secrete Cl- by activating both Ca2+-dependent and cAMP-dependent Cl- secretion mechanisms. VMH forms ion-permeable pores in the lipid bilayer that are non-selectively permeable to small ions. However, the ion permeability of these pores was not inhibited by glybenclamide and DIDS, and VMH did not change the cell membrane potential. These observations indicate that the pores formed on the cell membrane by VMH are unlikely to be involved in VMH-induced Cl- secretion. Notably, VMH stimulated fluid accumulation in the iliac loop test that was fully suppressed by a combination of DIDS and glybenclamide. Thus, Ca2+-dependent and cAMP-dependent Cl- secretion may be important therapeutic targets with regard to the diarrhoea that is induced by Vibrio mimicus.

    DOI: 10.1111/j.1462-5822.2006.00809.x

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  • Analysis of genetic determinants involved in multiresistance in clinical strains isolated from renal transplantation recipients in Cuangzhou, China

    Shi L, Kou Y, Li L, Miyoshi S

    J Health Sci   53 ( 2 )   185 - 189   2007年

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  • Vibrio vulnificus infection and metalloprotease

    Shin-ichi Miyoshi

    JOURNAL OF DERMATOLOGY   33 ( 9 )   589 - 595   2006年9月

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    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING  

    Vibrio vulnificus is ubiquitous in aquatic environments; however, it occasionally causes serious and often fatal infections in humans. These include invasive septicemia contracted through consumption of raw seafood, as well as wound infections acquired through contact with brackish or marine waters. In most cases of septicemia, the patients have underlying disease(s), such as liver dysfunction or alcoholic cirrhosis, and the secondary skin lesions including cellulitis, edema and hemorrhagic bulla appear on the limbs. Although V. Vul produces various virulent factors including polysaccharide capsule, type IV pili, hemolysin and proteolytic enzymes, the 45-kDa metalloprotease may be a causative factor of the skin lesions, because the purified protease enhances vascular permeability through generation of chemical mediators and also induces serious hemorrhagic damage through digestion of the vascular basement membrane. As well as other bacteria, V. Vul can regulate the protease production through the quorum-sensing system depending on bacterial cell density. However, this system operates efficiently at 25 degrees C, but not at 37 degrees C. Therefore, V. vulnificus may produce sufficient amounts of the protease only in the interstitial tissue of the limbs, in which temperature is lower than the internal temperature of the human body.

    DOI: 10.1111/j.1346-8138.2006.00139.x

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  • Biodegradation of dichloromethane by the polyvinyl alcohol-immobilized methylotrophic bacterium Ralstonia metallidurans PD11

    C Miyake-Nakayama, H Ikatsu, M Kashihara, M Tanaka, M Arita, S Miyoshi, S Shinoda

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   70 ( 5 )   625 - 630   2006年5月

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    記述言語:英語   出版者・発行元:SPRINGER  

    A dichloromethane (DCM)-degrading bacterium, Ralstonia metallidurans PD11 NBRC 101272, was immobilized in a polyvinyl alcohol (PVA) gel to use in a bioreactor for DCM treatment. After 4-month incubation of PVA gel beads with R. metallidurans PD11 and DCM in a mineral salt medium, the cells were tightly packed in the mesh of the gel. Forty beads of the gel in 10 ml of a batch system model showed effective activity degrading 500 and 1,000 mg l(-1) DCM within 2 and 3 h, respectively. Although reduction of pH due to accumulation of chloride ion liberated from DCM decreased the activity, it was recovered by adjustment to neutral pH. The activity of the immobilized cells was not affected by addition of nutrients which were preferentially utilized by R. metallidurans PD11, unlike the activity of the free-living cells. A continuous flow system with a column was more effective for rapid degradation of DCM. Thus, the PVA gel-immobilized cell of R. metallidurans PD11 is thought to be a prospective candidate to develop the bioreactor.

    DOI: 10.1007/s00253-005-0194-4

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  • Growth phase-dependent production of a toxic metalloprotease by Vibrio vulnificus

    SI Miyoshi, S Sultan, Y Yasuno, S Shinoda

    TOXIN REVIEWS   25 ( 1 )   19 - 30   2006年4月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS INC  

    Vibrio vulnificus, a ubiquitous estuarine bacterium, is divided into two groups based on the virulence potential. Group 1 is a causative agent of human fatal septicemia, which is characterized by edematous and hemorrhagic secondary skin lesions on the limbs. This human pathogen secretes a toxic metalloprotease as an important virulence determinant. The protease can evoke the skin damage through enhancement of the vascular permeability and destruction of the capillaries. Group 2 is an etiological agent of epizooticus in the cultured eels. Significant levels of metalloprotease as well as autoinducer, a well-known signal molecule of cell-density dependent regulatory system for production of virulence determinants, were found to be secreted by both the groups at early stationary phase but not middle logarithmic phase when the bacteria were cultivated at 25 degrees C. Expression of the protease gene also was found to be increased several times at the stationary phase. Therefore, the autoinducer molecules that had accumulated might have accelerated the expression of the protease gene. In contrast, when cultivated at 37 degrees C, far less amounts of the protease and autoinducer were produced even at the stationary phase. Most patients suffering from V. vulnificus septicemia have underlying diseases causing elevation of the serum iron level. When incubated in human serum supplemented with ferric chloride at 37 degrees C, only Group 1 V. vulnificus showed steady bacterial multiplication and protease production but secretion of significant level of autoinducer could not be detected. Hence, the protease production by Group 1 V. vulnificus in human serum containing ferric ion is also dependent on the bacterial growth; however, protease production in this condition does not require the accumulation of the autoinducer.

    DOI: 10.1080/15569540500320862

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  • Proteolytic activation of Vibrio mimicus (Vm) major outer membrane protein haemagglutinin (HA) with Vm-HA/protease: Implication for understanding bacterial adherence

    Munirul Alam, Shin-ichi Miyoshi, Kabir Uddin Ahmed, Nur A. Hasan, Ken-ichi Tomochika, Surnio Shinoda

    MICROBIOLOGY AND IMMUNOLOGY   50 ( 11 )   845 - 850   2006年

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    記述言語:英語   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    Vibrio mimicus (Vm) haemagglutinins (HAs), such as an extracellular HA/protease (Vm-HA/protease) and a major outer membrane protein-HA (Vm-OMPHA), have been recognized as the putative adherence factors for the bacterium. However, the mechanism by which HAs coordinate the adherence function of the bacterium remains as yet unknown. We report herein the positive interaction between Vm-HA/protease and Vm-OMPHA resulting in significant enhancement of the haemagglutinating ability. In this interaction, no cleaved polypeptide was detected; however, limited proteolysis of Vm-OMPHA was confirmed by SDS-PAGE. The proteolytic activation of the native cell-associated Vm-ONIPHA by limited proteolysis was also demonstrated in several V mimicus strains. Proteolytic activation of OMPRA was also achieved with various proteases from bacterial and eukaryotic sources. These findings may indicate a novel coordination of V mimicus HAs in the adherence of the bacterium.

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  • Presence of LuxS/AI-2 based quorum-sensing system in Vibrio mimicus: LuxO controls protease activity

    Sultan Z, Miyoshi S, Shinoda S

    Microbiol Immunol   50 ( 5 )   407 - 417   2006年

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  • 原因子としてのビブリオ属菌プロテアーゼ

    篠田純男, 三好伸一

    日本細菌学雑誌   61(2):261-271   2006年

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  • Molecular characterization of a multidrug-resistant strain of enteroinvasive Escherichia coli O164 isolated in Japan

    AM Ahmed, S Miyoshi, S Shinoda, T Shimamoto

    JOURNAL OF MEDICAL MICROBIOLOGY   54 ( 3 )   273 - 278   2005年3月

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    記述言語:英語   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Enteroinvasive Escherichia coli (EIEC) O164 strain RIMD05091045 was isolated from a travelling patient suffering from diarrhoea at the Osaka airport quarantine facility in Japan. The strain showed multidrug resistance against streptomycin, spectinomycin, co-trimoxazole (trimethoprim/sulfamethoxazole) and ampicillin, and reduced susceptibility to ciprofloxacin. Molecular characterization of the multidrug-resistance phenotype revealed the presence of a class 1 integron containing three genes, a dihydrofolate reductase type XII gene, dfrXII, which confers resistance to trimethoprim, an aminoglycoside adenyltransferase gene, aadA2, which confers resistance to streptomycin and spectinomycin, and an ORF of unknown function. Southern blot hybridization and conjugation experiments showed that the class 1 integron was located on a transferable plasm id that was less than 90 kb in size. The resistance of EIEC 0164 to ampicillin was found to be due to the presence of TEM-1 beta-lactamase. On the other hand, a single mutation that has not previously been described, P1 58-to-S, was detected downstream of the quinolone-resistance-determining region of parC of topoisomerase IV and may be responsible for the reduced susceptibility to ciprofloxacin in this strain.

    DOI: 10.1099/jmm.0.45908-0

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  • Evaluation of the biofilm-forming ability and genetic typing for clinical isolates of Pseudomonas aeruginosa by enterobacterial repetitive Intergenic consensus-based PCR

    WQ Yang, L Shi, WX Jia, XL Yin, JY Su, YL Kou, Yi, X, S Shinoda, S Miyoshi

    MICROBIOLOGY AND IMMUNOLOGY   49 ( 12 )   1057 - 1061   2005年

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Biofilm formation is an important phenotype associated with chronic Pseudomonas aeruginosa infections. In the present study, a total of 48 P. aeruginosa strains isolated from clinical specimens were examined for their biofilm-forming ability using a microtiter plate method. The different biofilm-forming abilities were demonstrated among the strains; however, most strains formed a larger biofilm than strain PAO1, a reference strain. The genetic typing was also carried out by enterobacterial repetitive intergenic consensus-based polymerase chain reaction. Although they were divided into five groups (A to E), most of the strains showing the higher biofilm-forming ability were found to be in groups D and E, suggesting a significant relationship between the biofilm-forming ability and the genetic group.

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  • A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce ATP and cyclic AMP which appear to be secretory mediators

    YS Li, K Okamoto, E Takahashi, S Miyoshi, S Shinoda, T Tsuji, Y Fujii

    MICROBIOLOGY AND IMMUNOLOGY   49 ( 1 )   73 - 78   2005年

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    記述言語:英語   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    The hemolysin of Vibrio mimicus (VMH) is a pore-forming toxin with both enterotoxiic and hemolytic activity. The hemolysis by VMH is induced by creation of pores in the membrane of erythrocyte; however, the mechanism for the enterotoxic action of VMH has remained unclear. In order to clarify the mechanism, we incubated T84 cells (a human colon carcinoma cell line) with VMH and found that the levels of ATP and cyclic AMP of culture medium increased after exposure of the cells to VMH. Subsequently. we found that the fluid accumulating activity of VMH in a mouse internal loop assay was reduced by administration of glibenclamide, an inhibitor of cyclic AMP-dependent chloride channels, into the intestinal loop. These results suggest that the stimulation of cells to produce nucleotides by VMH is linked to the enterotoxic activity of the toxin.

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  • The cytotoxin-hemolysin genes of human and eel pathogenic Vibrio vulnificus strains: Comparison of nucleotide sequences and application to the genetic grouping

    M Senoh, S Miyoshi, K Okamoto, B Fouz, C Amaro, S Shinoda

    MICROBIOLOGY AND IMMUNOLOGY   49 ( 6 )   513 - 519   2005年

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    記述言語:英語   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    Vibrio vulnificus can be divided into two groups on the basis of pathogenesis. Group 1 is pathogenic only to humans, whereas group 2 is pathogenic to eels and occasionally to humans. Although both groups produce a 50-kDa cytotoxin-hemolysin (V vulnificus hemolysin; VVH), the toxins are different. In the present study, the nucleotide sequence of the toxin gene (vvhA) of strain CDC B3547 (a group 2 strain) was determined, and the deduced amino acid sequence was compared to that of strain L-180 (a group I strain). The nucleotide sequence of vvhA of strain CDC B3547 was about 96 % identical with that of strain L-180, which results in a difference of 3 amino acid residues in the C-terminal lectin domain of VVH. Nevertheless, two primer sets for polymerase chain reaction could be designed to differentiate the toxin gene of each strain. When 27 V vulnificus clinical isolates were tested, group 1 strains (9 strains) were shown to react only to the primers designed for vvhA of strain L-180; whereas, the gene of group 2 strains (18 strains) could be amplified with the primers for vvhA of strain CDC B3547. These findings may lead to development of a novel genetic grouping system related to the virulence potential or to the host range.

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  • Generation of active fragments from human zymogens in the brady kinin-generating cascade by extracellular proteases from Vibrio vulnificus and V-parahaemolyticus

    S Miyoshi, H Watanabe, T Kawase, H Yamada, S Shinoda

    TOXICON   44 ( 8 )   887 - 893   2004年12月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Vibrio vulnificus is an opportunistic human pathogen causing septicemia, and the infection is characterized by formation of the edematous. skin lesions on limbs. This pathogenic species secretes a thermolysin-like metalloprotease as a virulence determinant. The metalloprotease was confirmed to activate human factor XII-plasma kallikrein-kinin cascade that results in liberation of bradykinin, a chemical mediator enhancing the vascular permeability, from high-molecular weight kininogen. Namely, the metalloprotease showed to generate active fragments by cleavage of Arg-Ile, Arg-Val or Gly-Leu peptide bond in human zymogens (plasma prekallikrein and factor XII). In spite of induction of the sufficient vascular permeability-enhancing and edema-forming reaction in the guinea pig model, a serine protease from V. parahaemolyticus, a human pathogen causing primarily watery diarrhea, showed far less ability to activate and to cleave the human zymogens. These results in part may explain why only V. vulnificus often causes serious edematous skin damages in humans. (C) 2004 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.toxicon.2004.08.013

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  • Regulation system for protease production in Vibrio vulnificus

    T Kawase, S Miyoshi, Z Sultan, S Shinoda

    FEMS MICROBIOLOGY LETTERS   240 ( 1 )   55 - 59   2004年11月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Vibrio vulnificus is a causative agent of serious food-borne diseases in humans related to consumption of raw seafoods. This human pathogen secretes a metalloprotease (VVP) that evokes enhancement of the vascular permeability and disruption of the capillaries. Production of microbial proteases is generally induced at early stationary phase of its growth. This cell density dependent regulation of VVP production in V vulnificus known to be the quorum-sensing. When V. vulnificus was cultivated in Luria-Bertani (LB) medium, accumulation of the autoinducer, the signal molecule operating the quorum-sensing system, was detected. Moreover, expression of the vvp gene encoding VVP was found to be closely related with expression of the luxS gene that encode the synthase of the autoinducer precursor (luxS). These findings may indicate VVP production is controlled by the quorum-sensing system in LB medium. Futhermore, this system functioned more effectively at 26 degreesC than at 37 degreesC. When incubated at 37 degreesC in human serum supplemented with ferric chloride, production of VVP and expression of vvp increased in proportion to the concentration of ferric ion; whereas, expression of luxS was not increased. This suggests that VVP production in human serum containing ferric ion may be regulated mainly by the system other than the quorum-sensing system. (C) 2604 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.femsle.2004.09.023

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  • High growing ability of Vibrio vulnificus biotype 1 is essential for production of a toxic metalloprotease causing systemic diseases in humans

    H Watanabe, S Miyoshi, T Kawase, K Tomochika, S Shinoda

    MICROBIAL PATHOGENESIS   36 ( 3 )   117 - 123   2004年3月

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    記述言語:英語   出版者・発行元:ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD  

    Vibrio vulnificus biotype 1, a causative agent of fatal septicemia or wound infection in humans, is known to produce a toxic metalloprotease as an important virulence determinant. V. vulnificus biotype 2 (serovar E), a primary eel pathogen, was found to elaborate an extracellular metalloprotease that was indistinguishable from that of biotype 1. The potential of V. vulnificus biotype 1 for production of the metalloprotease was compared with biotype 2 and other human non-pathogenic Vibrio species (Vibrio anguillarum and Vibrio proteolyticus). When cultivated at 25degreesC in tryptone-yeast extract broth supplemented with 0.9% NaCl, all bacteria multiplied sufficiently and secreted significant amounts of the metalloprotease. However, at 37degreesC with 0.9% NaCl, V. anguillarum neither grew nor produced the metalloprotease. In human serum, only V. vulnificus biotype 1 revealed a steady multiplication accompanied with production of the extracellular metalloprotease. This prominent ability of biotype 1 in growth and protease production may contribute to cause serious systemic diseases in humans. (C) 2003 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.micpath.2003.10.001

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  • An exocellular cytolysin produced by Vibrio vulnificus CDC B3547, a clinical isolate in biotype 2 (Serovar E)

    SI Miyoshi, A Morita, T Teranishi, KI Tomochika, S Yamamoto, S Shinoda

    JOURNAL OF TOXICOLOGY-TOXIN REVIEWS   23 ( 1 )   111 - 121   2004年

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    記述言語:英語   出版者・発行元:MARCEL DEKKER INC  

    Vibrio vulnificus biotype 2, a primary eel pathogen, is also an opportunistic pathogen for humans. The strains in this biotype secrete a cytolysin into the culture medium. The cytolysin from the strain CDC B3547 (ATCC 33817), which was originally isolated from a human leg wound, can disrupt various kinds of eukaryotic cells including erythrocytes and mast cells, and artificial vesicles, liposomes. The cytolysin is a 50 kDa single-chain protein and is categorized into the pore-forming toxins. After binding tightly to the cell-membrane cholesterol in a temperature-independent manner, the toxin molecules assemble each other in a temperature-dependent manner, forming a small transmembrane pore. When incubated with a metalloprotease from the same species, the cytolysin is converted to the nicked toxin composed of some peptide chains, joined with disulfide bond(s). This nicked toxin is more hydrophilic while maintaining comparable cytolytic activity.

    DOI: 10.1081/TXR-120030650

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  • Distribution of virulence-associated genes in Vibrio mimicus isolates from clinical and environmental origins

    Shinoda S, Nakagawa T, Shi L, Bi K, Kanoh Y, Tomochika K, Miyoshi S, Shimada T

    Microbiol Immunol   2004年

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  • Identification and characterization of class 1 integron resistance gene cassettes amoung Salmonella strains isolated from healthy humans in China

    Zhang H, Shi L, Li L, Guo S, Zhang X, Yamasaki S, Miyoshi S, Shinoda S

    Microbiol Immunol   2004年

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  • Isolation and characterization of 1,3-dichloro-2-propanol-degrading bacterium

    Yonetani R, Ikatsu H, Miyake-Nakayama C, Fujiwara E, Maehara Y, Miyoshi S, Matsuoka H, Shinoda S

    J Health Sci   2004年

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  • Isolation and characterization of a new dichloromethane degrading bacterium, Ralstonia metallidurans, PD11

    Chizuko Miyake-Nakayama, Sachiyo Masujima, Hisayoshi Ikatsu, Shin-Ichi Miyoshi, Sumio Shinoda

    Biocontrol Science   9 ( 4 )   89 - 93   2004年

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    記述言語:英語   出版者・発行元:Society for Antibacterial and Antifungal Agents Japan  

    The widely used organic solvent dichloromethane (DCM) has various toxic effects including carcinogenesis. We isolated a DCM-degrading bacterium Ralstonia metallidurans PD11 from drainage water which grew with DCM as a sole carbon source. PD11 was a methylotrophic bacterium with the ability to grow with C1 compounds such as methanol or methylamine. Although the existence of methylotrophic bacteria having DCM-degrading ability has been reported, there has been no report on Ralstonia sp. to date. The DCM-degrading activity of PD11 was increased by acclimatization, finally reaching a level to degrade 2,500 mg DCM/l within a week. The cell-free extract of PD11 showed DCM-degrading activity by liberating chloride which was stimulated by addition of glutathione, suggesting that the DCM dehalogenationg enzyme could be classified into the glutathione S-transferase super family.

    DOI: 10.4265/bio.9.89

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  • Identification and characterization of genes required for biosynthesis and transport of the siderophore vibrioferrin in Vibrio parahaemolyticus

    T Tanabe, T Funahashi, H Nakao, SI Miyoshi, S Shinoda, S Yamamoto

    JOURNAL OF BACTERIOLOGY   185 ( 23 )   6938 - 6949   2003年12月

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    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    In response to low iron availability, Vibrio parahaemolyticus synthesizes and secretes a polyhydroxycarboxylate-type siderophore vibrioferrin which is composed of 1 mol each of 2-ketoglutaric acid, L-alanine, ethanolamine, and citric acid. We have previously reported the cloning and characterization of the pvuA gene, which encodes the 78-kDa outer membrane receptor protein for ferric vibrioferrin. In this study, nine genes involved in the biosynthesis and transport of vibrioferrin have been identified in the genomic regions surrounding the pvuA gene. The genes were sequenced, and gene disruptants were constructed by insertion mutation for phenotype analysis. Five of the genes, named pvsABCDE, constitute an operon that is expressed under iron-limiting conditions. Homology searches of their predicted protein products suggested that the four genes pvsABDE are implicated in the biosynthesis of the siderophore. Another gene in the same operon,pvsC, encodes a putative exporter that is homologous to members of the major facilitator superfamily of multidrug efflux pumps. The remaining four genes, named pvuBCDE, encode proteins strongly homologous to Escherichia coli FecBCDE, respectively, which are components of the ATP-binding cassette transporter system for ferric dicitrate. Reverse transcriptase PCR analysis revealed that these transport genes are transcribed as a single mRNA with the upstream genes, psuA and pvuA. Phenotypic comparison between the wild-type strain and its targeted gene disruptants supported the biological functions for the respective operons that were expected on the basis of the homology search.

    DOI: 10.1128/JB.185.23.6938-6949.2003

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  • Histamine-releasing reaction induced by the N-terminal domain of Vibrio vulnificus metalloprotease

    S Miyoshi, K Kawata, M Hosokawa, K Tomochika, S Shinoda

    LIFE SCIENCES   72 ( 20 )   2235 - 2242   2003年4月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    A zinc metalloprotease secreted by Vibrio vulnificus, an opportunistic human pathogen causing septicemia and wound infection, stimulates exocytotic histamine release from rat mast cells. This protease consists of two functional domains: the N-terminal domain that catalyzes proteolytic reaction and the C-terminal domain that promotes the association with a protein substrate or cell membrane. Like the intact protease, the N-terminal domain alone also induced histamine release from rat peritoneal mast cells in a dose- and time-dependent manner. However, the reaction induced was apparently weak and went on more slowly. The nickel-substituted protease or its N-terminal domain, each of which has the reduced proteolytic activity due to decreased affinity to a substrate, showed much less histamine-releasing activity. When injected into the rat dorsal skin, the N-terminal domain also evoked enhancement of the hypodermic vascular permeability, while the activity was comparable to that of the protease. Taken together, the protease may stimulate histamine release through the action of the catalytic center of the N-terminal domain on the target substance(s) on the mast cell membrane. The C-terminal domain may support the in vitro action of the N-terminal domain by coordination of the association of the protease with the membrane, but it may not modulate the in vivo action. (C) 2003 Elsevier Science Inc. All rights reserved.

    DOI: 10.1016/S0024-3205(03)00094-8

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  • Vibrio vulnificus induces macrophage apoptosis in vitro and in vivo

    T Kashimoto, S Ueno, M Hanajima, H Hayashi, Y Akeda, S Miyoshi, T Hongo, T Honda, N Susa

    INFECTION AND IMMUNITY   71 ( 1 )   533 - 535   2003年1月

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    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    In this study, we compared the apoptotic activities of clinical and environmental isolates of Vibrio vulnificus toward macrophages in vitro and in vivo. The clinical isolates induced apoptosis in macrophage-like cells in vitro and in macrophages in vivo. This suggests that macrophage apoptosis may be important for the clinical virulence of V. vulnificus.

    DOI: 10.1128/IAI.71.1.533-535.2003

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  • Evidence that temporally alternative expression of the Vibrio vulnificus elastase prevents proteolytic inactivation of hemolysin

    Rhee JE, Lee JH, Jeog HS, Park U, Lee DH, Woo GJ, Miyoshi S, Dhoi SH

    J Microbiol Biotechnol   2003年

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  • Studies on pathogenic Vibrio parahaemolyticus during a warm weather season in the Seto Inlnd Sea, Japan

    Alam MJ, Miyoshi S, Shinoda S

    Environ Microbiol   2003年

  • An exocellular thermolysin-like metalloprotease produced by Vibrio fluvialis: purification, characterization, and gene cloning

    S Miyoshi, Y Sonoda, H Wakiyama, MM Rahman, K Tomochika, S Shinoda, S Yamamoto, K Tobe

    MICROBIAL PATHOGENESIS   33 ( 3 )   127 - 134   2002年9月

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    記述言語:英語   出版者・発行元:ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD  

    An exocellular metalloprotease produced by Vibrio fluvialis, an enteropathogenic vibrio, was purified and characterized. The metalloprotease (V. fluvialis protease [VFP]) was found to have very similar characteristics to V. vulnificus protease, including a molecular mass of 45 kDa, sensitivity to chelating agents or competitive inhibitors for thermolysin-like metalloproteases, and the substrate specificity. The structural gene for VFP was also cloned, and its nucleotide sequence was determined. The deduced amino acid sequence confirmed that VFP was a member of the thermolysin family. VFP, like V. vulnificus protease, showed the haemagglutinating, permeability-enhancing and haemorrhagic activities in addition to the proteolytic activity toward oligopeptide, casein or elastin. (C) 2002 Elsevier Science Ltd. All rights reserved.

    DOI: 10.1006/mpat.2002.0520

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  • ビブリオのVNC菌とその衛生学的問題

    友近健一, 三好伸一, 篠田純男

    Bokin Bobai   30(2):85-90   2002年

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  • Identification and characterization of pvuA, a cene encoding the ferric vibrioferrin receptor protein in Vibrio parahaemolyticus

    Funahashi T, Moriya K, Uemura S, Miyoshi S, Shinoda S, Narimatsu S, Yamamoto S

    J Bacteriol   2002年

  • Specificity of a heme-assimilating system of Vibrio vulnificus to synthetic heme compounds

    Miyoshi S, Kamei T, Ota Y, Masunaga C, Izuhara Y, Tomochika K, Shinoda S, Yamamoto S

    FEMS Microbiol Lett   2002年

  • Induction of an outer membrane proteine of 78 kDa in Vibrio vulnificus cultured in the presence of desfferioxamine B under iron-limiting conditions

    Aso H, Miyoshi S, Nakao H, Okamoto K, Yamamoto S

    FEME Microbiol Lett   2002年

  • Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan

    Alam MJ, Tomochika K, Miyoshi S, Shinoda S

    FEMS Microbiol Lett   2002年

  • Purification of a serine protease of Vibrio parahaemolyticus and its characterization

    Ishihara M, Kawanishi A, WAtanabe H, Tomochika K, Miyoshi S, Shinoda S

    Microbiol Immunol   2002年

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  • Identification and characterization of the sodA genes encoding manganese superoxide dismutase in Vibrio parahaemolyticus, Vibrio mimicus, and Vibrio vulnificus

    Kimoto R, Funahashi T, Yamamoto N, Miyoshi S, Narimatsu S, Yamamoto S

    Microbiol Immunol   2001年

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  • The C-terminal domain promotes the hemorrhagic damage caused by Vibrio vulnificus metalloprotease

    Miyoshi S, Kawata K, Tomochika K, Shinoda S, Yamamoto S

    Toxicon   2001年

  • Detection of viable Vibrio mimicus by reverse transcription-polymerase chain reaction

    K. Bi, S. I. Miyoshi, L. Shi, K. I. Tomochika, S. Shinoda

    Biocontrol Science   6 ( 2 )   81 - 86   2001年

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    記述言語:英語   出版者・発行元:Society for Antibacterial and Antifungal Agents Japan  

    Differentiating viable cells from nonviable cells is of considerable importance in the monitoring of food-borne pathogens. A reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to detect mRNA from the phospholipase gene (phl) of Vibrio mimicus. Viable V. mimicus cells were killed by heat or ethanol treatment and kept for various periods at room temperature. Total RNA from V. mimicus was extracted, treated with DNase and subjected to RT-PCR with primers for the phl gene. The phl mRNA was detected in the viable cells, but it gradually disappeared when the killed cells were left at room temperature and became undetectable after 8 h of storage. Furthermore, RT-PCR generated a 493 bp fragment from the total RNA extracted from as few as about 103 organisms, confirming the sensitivity of the assay. The amplification of the phl mRNA was specific for V. mimicus, as no amplification was found when fifteen other Vibrio species and seven related organisms were tested. The results indicated a good relationship between the detection of the phl mRNA and viability of V. mimicus cells because the phl transcript is rapidly degraded upon cell death. This work shows the usefulness of RT-PCR as a sensitive method for the specific detection of viable V. mimicus.

    DOI: 10.4265/bio.6.81

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  • Analysis of seawaters for the recovery of culturable Vibrio parahaemolyticus and some other vibrios

    Alam MJ, Tomochika K, Miyoshi S, Shinoda S

    Microbiol Immunol   2001年

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  • Detection of virulence associated genes in clinical strains of Vibrio mimicus

    Bi K, Miyoshi S, Tomochika K, Shinoda S

    Microbiol Immunol   2001年

  • Presence of hemolysin genes (vmh, tdh, trh) in isolates of Vibrio mimicus: determined by polymerase chain reaction

    Shi L, Miyoshi S, Bi K, Nakamura M, Hiura M, Tomochika K, Shinoda S

    J Health Sci   2000年

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  • Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction

    Bi K, Shi L, Maehara Y, Miyoshi S, Tomochika K, Shinoda S

    Microbiol Immunol   2000年

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  • Cloning and characterization of the ddc homolog encoding L-2,4-diaminobutyrate decarboxylase in Enterobacter aerogenes

    Yamamoto S, Mutoh N, Tsuzuki D, Ikai H, Nakao H, Shinoda S, Narimatsu S, Miyoshi S

    Biol Pharm Bull   2000年

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  • Characterization of Vibrio parahaemolyticus manganese-resistant mutants in reference to the function of the ferric uptake regulatory protein

    Funahashi T, Fujiwara C, Okada M, Miyoshi S, Shinoda S, Narimatsu S, Yamamoto S

    Microbiol Immunol   2000年

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  • Isolation and characterization of cytochrome P450-producing bacteria from various environments

    H. Ikatsu, Y. Kino, N. Kawahara, M. Adachi, S. I. Miyoshi, K. I. Tomochika, S. Shinoda

    Biocontrol Science   5 ( 2 )   111 - 116   2000年

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    記述言語:英語   出版者・発行元:Society for Antibacterial and Antifungal Agents Japan  

    Eight strains of cytochrome P450 (P450)-producing bacteria were isolated from M9 medium containing a P450-inducer as the sole carbon source from the environment. Strains EP1 to EP6 utilizing 2-ethoxyphenol as the sole carbon source were isolated from the soil of a weed-filled field, the water of a paddy field, laboratory effluent, domestic effluent and river water. Strain MP1 utilizing 2-methoxyphenol and strain CP1 utilizing camphor were isolated from oil-polluted soil and the soil of a weed-filled field, respectively. This suggests the distribution of P450-producing bacteria in the environment. Metyrapone (2-methyl-1,2-di-3-pyridyl-1-propanone) inhibited the growth of P450-producing bacteria on the media containing a P450-inducer as the sole carbon source, strongly suggesting that the P450 is involved in the catabolism of the inducer.

    DOI: 10.4265/bio.5.111

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  • A snake venom inhibitor to muscarinic acetylcholine receptor (mAChR): isolation and interaction with cloned human mAChR

    Miyoshi S, Tu AT

    Arch Biochem Biophys   2000年

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  • Isolation of dichloromethane-degrading bacteria from drainage water

    Kawata H, Nakayama C, Sakamoto M, Ikatsu H, Miyoshi S, Tomochika K, Shinoda S

    J Health Sci   2000年

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  • Microbial metalloproteases and pathogenesis

    Miyoshi S, Shinoda S

    Microbes Infect   2:91-98   2000年

  • ビブリオ金属プロテアーゼの微小循環系に対する作用とその制御

    三好伸一

    Yakugaku Zasshi   120:1149-1157   2000年

  • Dichloromethane-degrading properties of bacteria isolated from environmental water

    H. Ikatsu, H. Kawata, C. Nakayama, S. I. Miyoshi, K. I. Tomochika, T. Katsu, S. Shinoda

    Biocontrol Science   5 ( 2 )   117 - 120   2000年

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    記述言語:英語   出版者・発行元:Society for Antibacterial and Antifungal Agents Japan  

    Degradation of dichloromethane (DCM) by two environmental isolates, Flevimones sp. strain P3310 and Chryseobacterum sp. strain G31, were studied. The ability of the strains was raised to degrade 3,000 mg/l of DCM by acclimatization, although the original isolates could degrade less than 500 mg/l. The first step in the degradation process was dechlorination, and the liberated chloride ions caused the reduction of pH and the bacterial growth
    the addition of phosphate salts, however, restored the growth and the degrading ability of the culture by increasing the buffer capacity. The DCM-degrading activity was also detected in the cell-free extract and the culture-supernatant. These results suggest that the isolates or their products are possible candidates for bioremediation to eliminate DCM pollution.

    DOI: 10.4265/bio.5.117

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  • Enteropathogenic factors produced by vibrios other than cholera toxin

    Shinoda S, Miyoshi S

    J Nat Toxins   9:231-250   2000年

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  • Purification and characterization of 2-ethoxyphenol-induced cytochrome P450 from Corynebacterium sp. strain EP1

    Kawahara N, Ikatsu H, Kawata H, Miyoshi S, Tomochika K, Shinoda S

    Can J Microbiol   1999年

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  • The hemagglutinating action of Vibrio vulnificus metalloprotease

    Miyoshi S, Kawata K, Tomochika K, Shinoda S

    Microbiol Immunol   1999年

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  • The ability of Vibrio vulnificus to use a synthetic hydrophilic heme compound, Fe-TPPS, as a single iron source

    Miyoshi S, Kamei T, Inami Y, Ota Y, Yamamoto S, Tomochika K, Shinoda S

    FEMS Microbiol Lett   1999年

  • Siderophore production of Vibrio parahaemolyticus strains from differnt sources

    Yamamoto S, Okujo N, Miyoshi S, Shinoda S, Narimatsu S

    Microbiol Immunol   1999年

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  • Vibrio vulnificusの亜鉛金属プロテアーゼに関する研究

    三好伸一

    日本細菌学雑誌   54(4):763-772   1999年

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  • Muscarinic acetylcholine receptor (mAChR) inhibitor from snake venom: interaction with subtypes of human mAChR

    Miyoshi S, Tu AT

    Arch Biochem Biophys   1999年

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▼全件表示

講演・口頭発表等

  • Analysis of extracellular proteases of bacteria which inhabit aquatic environments

    The 14th Asian Coference on Diarrhoeal Disease and Nutrition  2017年 

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  • Effects of disruption of genes expressed during VBNC phase of Vibrio cholerae on survival under starvation

    The 19th International Conference on Emerging Infectious Diseases (EID) in the Pacific Rim. US-Japan Joint Panel Conference on Cholera and Other Bacterial Enteric Infections  2017年 

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  • コルカタ市で単離されたVibrio choleraeの環境分離株及び臨床分離株の病原因子の比較研究

    第90回日本細菌学会総会  2017年 

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  • Analysis of antiboitic resistant gene variation in Vibrio cholerae isolated from clinical patients and environmental water in Kolkata from 2007-2014

    IUMS 15th International Congress of Bacteriology and Applied Microbiology  2017年 

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  • Production of extracellular proteases of bacteria which inhabit aquatic environments

    IUMS 15th International Congress of Bacteriology and Applied Microbiology  2017年 

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  • Effects of disruption of genes expressed during VBNC phase of Vibrio cholerae on survival under starvation

    第90回日本細菌学会総会  2017年 

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  • コルカタ市において臨床及び環境水より分離されたVibrio choleraeの保有する抗薬剤耐性遺伝子の解析

    第90回日本細菌学会総会  2017年 

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  • Comparison of proteome profiles between culture, viable but non-culturable (VBNC) and recovery state in Vibrio cholerae

    第16回あわじしま感染症・免疫フォーラム  2017年 

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  • 岡山市内環境水のコレラ菌を含むビブリオ・コレレ汚染に関する研究

    第44回日本防菌防黴学会年次大会  2017年 

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  • Characterization of the viable but non-culturable (VBNC) and recovery state in Vibrio cholerae

    日米コレラ部会日本側総会  2017年 

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  • Serratia marcescensにおけるクロルヘキシジンに対する馴化・耐性機構の解析

    第29回微生物シンポジウム  2017年 

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  • 災害時における食中毒とその対策について

    第44回日本防菌防黴学会年次大会  2017年 

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  • コルカタ市で分離されたVibrio cholerae O1の保有する薬剤耐性遺伝子の解析

    第70回日本細菌学会中国・四国支部総会  2017年 

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  • Vibrio choleraeの保有する可動性伝達因子SXT elementの解析

    第51回ビブリオシンポジウム  2017年 

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  • Properties of exotoxins produced by Aeromonas species

    Gut Microbiome 2016: an international perspective  2016年 

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  • Comparative genomic analysis reveals heterogeneity in VSP-II genomic island of El Tor variant Vibrio cholerae in Kolkata, India

    50th US-Japan Cooperative Medical Science Program Conference on Cholera and Other Bacterial Enteric Infections  2016年 

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  • Whole genome analysis reveals heterogeneity of VSP-II and genetic shifts of Vibrio cholerae O1 clinical isolates in Kolkata India

    The 7th Vibrio conference 2016  2016年 

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  • コルカタにおける2007-2014年コレラ流行株の全ゲノム解析によって明らかになったVSP-IIの変化

    第89回日本細菌学会総会  2016年 

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  • コルカタ市環境水から単離されたVibiro choleraeのVPIとCTXΦの多様性

    第89回日本細菌学会総会  2016年 

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  • Functional role of N- and C-terminal amino acids in the structural subunits of colonization factor CS6 expressed by enterotoxigenic Escherichia coli

    The 15th Awaji International Forum on Infection and Immunity  2016年 

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  • コレラ菌を含むビブリオ・コレレの水環境汚染に関する日印両国での比較研究

    日本防菌防黴学会第43回年次大会  2016年 

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  • Modulation of the bacterial virulence by proteolytic enzymes

    The 7th Vibrio conference 2016  2016年 

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  • Effects of disruption of genes expressed during VBNC phase of Vibrio cholerae on survival under starvation

    日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会  2016年 

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  • インド・コルカタにおけるコレラ流行株の特徴と変化

    第50回腸炎ビブリオシンポジウム  2016年 

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  • 腸炎ビブリオのNa+耐性機構に関わる遺伝子の同定

    第50回腸炎ビブリオシンポジウム  2016年 

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  • 環境水由来のVibrio cholerae環境分離株のPathogenicity islandの多様性

    日本防菌防黴学会第43回年次大会  2016年 

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  • 腸炎ビブリオの新規抗菌物質排出ポンプの解析

    第69回日本細菌学会中国・四国支部総会  2016年 

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  • コルカタ市環境水から単離されたVibiro choleraeのVPIの多様性

    第49回腸炎ビブリオシンポジウム  2015年 

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  • すぐそばにいる微生物

    日本防菌防黴学会第42回年次大会  2015年 

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  • インドコルカタ市の環境水から単離されたVibrio choleraeのtcpA配列の多様性

    日本防菌防黴学会第42回年次大会  2015年 

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  • コルカタにおける2007-2014年コレラ流行株の全ゲノム解析によって明らかになったVSP-IIの変化

    第68回日本細菌学会中国・四国支部総会  2015年 

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  • ランドリー用洗剤に抵抗性を示す土壌細菌の単離・同定と性状解析

    日本防菌防黴学会第42回年次大会  2015年 

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  • Whole genome analysis reveals VSP-II genotype shift of Vibrio cholerae O1 clinical isolates between 2007 and 2014 in Kolkata, India

    Microcon-2015  2015年 

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  • 岡山市内の環境水のビブリオ・コレレおよびビブリオ属細菌汚染に関する調査研究

    第68回日本細菌学会中国・四国支部総会  2015年 

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  • VBNC状態に特徴的なコレラ菌タンパク質の探索

    第88回日本細菌学会総会  2015年 

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  • VBNC stage specific proteins in Vibrio cholerae

    49th US-Japan Cooperative Medical Science Program Conference on Cholera and Other Bacterial Enteric Infections  2015年 

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  • Inductive effect of skim milk on the production of serine protease by Aeromonas spp

    49th US-Japan Cooperative Medical Science Program Conference on Cholera and Other Bacterial Enteric Infections  2015年 

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  • Inductive effect of casein phophopeptide on the production of serine protease by Aeromonas spp

    第88回日本細菌学会総会  2015年 

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  • Whole genome analysis reveals VSP-II genotype shift of Vibrio cholerae O1 clinical isolates between 2007 and 2014 in Kolkata, India

    日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会  2015年 

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  • インドコルカタ市の環境水からのVibrio choleraeの単離と保有遺伝子解析

    第88回日本細菌学会総会  2015年 

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  • インドコルカタ市の環境水中から単離されたVibrio choleraeの保有遺伝子解析

    第48回腸炎ビブリオシンポジウム  2014年 

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  • Isolation of VBNC Vibrio cholerae from environmental water sample in Kolkata, India

    Asian-African Research Forum on Emerging and Reemerging Infections 2013  2014年 

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  • Analysis of outer membrane proteins and extracellular proteins of Vibrio mimicus incubated sub-lytic doses of antimicrobial peptides

    Asian-African Research Forum on Emerging and Reemerging Infections 2013  2014年 

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  • Time course changes and conversion of VBNC Vibrio cholerae, a suggested state in environmental water

    Asian-African Research Forum on Emerging and Reemerging Infections 2013  2014年 

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  • Vibrio choleraeの環境中での細胞状態と、VBNC細胞の変化に関する研究

    第87回日本細菌学会総会  2014年 

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  • Time course changes and conversion of VBNC Vibrio cholerae, a suggested state in environmental water

    48th US-Japan Cooperative Medical Science Program Conference on Cholera and Other Bacterial Enteric Infections  2014年 

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  • Analysis of outer membrane proteins produced by human pathogenic vibrios incubated with sub-lytic doses of antimicrobial peptides

    48th US-Japan Cooperative Medical Science Program Conference on Cholera and Other Bacterial Enteric Infections  2014年 

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  • 微生物試験法 食中毒菌の個別試験法 腸管出血性大腸菌

    日本薬学会第134年会  2014年 

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  • Isolation of VBNC Vibrio cholerae from environmental water sample, Kolkata, India, 2013

    International Union of Microbiological Societies Congresses 2014  2014年 

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  • クドア・セプテンプンクタータ試験法

    日本薬学会第134年会  2014年 

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  • 微生物試験法 汚染指標細菌試験法 腸内細菌科菌群

    日本薬学会第134年会  2014年 

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  • Inductive effect of skim milk on the production of serine protease by Aeromonas spp

    日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会  2014年 

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  • Aeromonas sobriaセリンプロテアーゼのスキムミルクによる産生亢進

    第61回トキシンシンポジウム  2014年 

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  • Characterization of the viable but nonculturable state in Vibrio cholerae

    International Union of Microbiological Societies Congresses 2014  2014年 

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  • VBNC stage specific proteins in Vibrio cholerae

    日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会  2014年 

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  • インドコルカタ市の環境水から単離されたVBNC Vibrio cholerae

    日本防菌防黴学会第41回年次大会  2014年 

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  • ジクロロメタン分解菌が保有する特異的遺伝子の解析

    日本防菌防黴学会第41回年次大会  2014年 

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  • Aeromonas sobria溶血毒に対するインドロキノリン化合物活性抑制作用

    第67回日本細菌学会中国・四国支部総会  2014年 

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  • コレラ菌のVBNC状態に特徴的なタンパク質の探索

    第67回日本細菌学会中国・四国支部総会  2014年 

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  • インドコルカタ市環境水からのHT-29細胞破砕抽出物を用いたVBNCコレラ菌の単離

    第47回腸炎ビブリオシンポジウム  2013年 

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  • Detection of VBNC Vibrio cholerae from the environmental water in Kolkata, India

    Asian-African Research Forum on Emerging and Reemerging Infections 2013  2013年 

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  • ランプ法を用いた食品中での各種食中毒微生物病原因子遺伝子の検出

    日本薬学会第133年会  2013年 

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  • 微生物試験法 環境病原性細菌試験法 リステリア・モノサイトゲネス

    日本薬学会第133年会  2013年 

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  • Modulation of Vibrio mimicus infection by human antimicrobial peptides

    Asian-African Research Forum on Emerging and Reemerging Infections 2013  2013年 

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  • インドコルカタ市における環境水からのVBNC Vibrio cholerae の単離

    第86回日本細菌学会総会  2013年 

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  • 環境水中のVibrio choleraeが形成していると考えられるVBNC細胞の経時的変化

    日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会  2013年 

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  • 亜致死量抗菌ペプチドに曝された病原ビブリオの外膜タンパク質の解析

    日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会  2013年 

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  • 食中毒にかからないために

    日本防菌防黴学会第41回通常総会及び付設講演会  2013年 

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  • Isolation of VBNC Vibrio cholerae from environmental water sample

    5th Congress of European Microbiologists (FEMS)  2013年 

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  • クリーニング用洗剤および溶剤に含まれる化学物質に抵抗性を示す環境微生物の解析

    日本防菌防黴学会第40回年次大会  2013年 

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  • 環境水中のVibrio choleraeが形成していると考えられるVBNC細胞の経時的変化

    第66回日本細菌学会中国・四国支部総会  2013年 

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  • インドコルカタ地域環境水中のVBNC Vibrio choleraeの生態

    日本防菌防黴学会第40回年次大会  2013年 

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  • Significance of Vibrio mimicus trypsin-like protease (VmtA) for maturation of Vibrio mimicus hemolysin

    Asian-African Research Forum on Emerging and Reemerging Infections 2012  2012年 

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  • VBNCコレラ菌のヒト結腸上皮細胞HT-29由来のconversion factor

    第24回微生物シンポジウム  2012年 

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  • ヒト腸管抗菌ペプチドのビブリオ感染症に対する抑制効果

    日本防菌防黴学会第39回年次大会  2012年 

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  • 腸炎ビブリオの鉄獲得系と生体内蔵職能の検討,及び抗ビブリオフェリン受容体モノクローナル抗体の作成

    第85回日本細菌学会総会  2012年 

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  • Defensive effects of human antimicrobial peptide on infectious diseases by vibrios

    日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会  2012年 

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  • Quorum sensing regulation of virulence factors expression in Vibrio vulnificus

    Bioactive Okayama: Food and Health, Okayama, Japan  2012年 

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  • Defensive effects of human antimicrobial peptide on infectious diseases by vibrios

    47th US-Japan Cooperative Medical Science Program Conference on Cholera and Other Bacterial Enteric Infections  2012年 

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  • A trypsin-like serine protease of Vibrio mimicus involved in maturation of a heat-labile hemolysin

    International Union of Microbiological Societies 2011 Congress  2011年 

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  • Assimilation of ferric ions bound to porphyrins by Vibrio vulnificus, the human and eel pathogen inhabitting estuarine and marine environments

    International Union of Microbiological Societies 2011 Congress  2011年 

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  • ビブリオ属細菌に対するヒト抗菌ペプチドの感染症抑制効果

    第32回日本食品微生物学会学術総会  2011年 

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  • A hemolytic factor other than Vibrio mimicus hemolysin (VMH) produced by a clinical strain

    46th US-Japan Cholera and Other Bacterial Enteric Infections Joint Panel Meeting 2011  2011年 

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  • ビブリオ・ミミカスにおけるトリプシン様プロテアーゼ遺伝子(vmtA)の保有状況と溶血毒素の活性化に対する影響

    第64回日本細菌学会中国・四国支部総会  2011年 

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  • ビブリオ属細菌に対するヒト抗菌ペプチドの感染症抑制効果

    第50回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会  2011年 

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  • 微生物試験法 核酸増幅法 細菌および真菌のDNA塩基配列解析による同定法

    日本薬学会第131年会  2011年 

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  • A hemolytic factor other than Vibrio mimicus hemolysin (VMH) produced by a clinical strain

    日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会  2011年 

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  • 家庭用洗剤に含まれる界面活性剤の環境微生物への影響

    日本防菌防黴学会第38回年次大会  2011年 

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  • 微生物試験法 汚染指標細菌試験法 腸内細菌科

    日本薬学会第131年会  2011年 

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  • 腸炎ビブリオのvibrioferrinを介する鉄獲得系と病原性

    第63回日本細菌学会中国・四国支部総会  2010年 

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  • Purification and characterization of Vibrio vulnificus serine protease (VvsA): a potential pathogenic factor

    The 3rd International Symposium for Future Technology Creating Better Human Health and Society  2010年 

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  • Vibrio mimicusヘモリジンの成熟化に関与する菌体外プロテアーゼ

    第57回トキシンシンポジウム  2010年 

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  • ビブリオ・バルニフィカスの産生する溶血毒素の変異導入によるトキソイド化

    日本防菌防黴学会第37回年次大会  2010年 

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  • Role of Vibrio mimicus trypsin-like protease in maturation of an enterotoxic hemolysin

    2010年度日米医学協力研究会 コレラ・細菌性腸管感染症専門部会 日本側総会  2010年 

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  • ビブリオ・ミミカス溶血毒素の成熟化過程

    第22回微生物シンポジウム  2010年 

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  • A novel endogenous protease involved in maturation of Vibrio mimicus hemolysin

    Asia-African Research Forum on Emerging and Reemerging Infections 2010  2010年 

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  • Role of Vibrio mimicus trypsin-like protease in maturation of an enterotoxic hemolysin

    45th Joint Conference on Cholera and Other Bacterial Enteric Infections Panel  2010年 

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  • ヒト抗菌ペプチドのビブリオ属細菌に対する影響

    第63回日本細菌学会中国・四国支部総会  2010年 

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  • ドメイン連結部分の変異によるVibrio vulnificus溶血毒素のトキソイド化

    第43回腸炎ビブリオシンポジウム  2009年 

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  • Vibrio minicusの産生する毒素活性化作用を有する新奇プロテアーゼ

    第82回日本細菌学会総会  2009年 

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  • 抗菌ペプチドの腸球菌Enterococcus faecalisの生存に及ぼす影響

    日本防菌防黴学会第36回年次大会  2009年 

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  • Vibrio mimicusヘモリジンの成熟化に関与する新奇プロテアーゼ

    第62回日本細菌学会中国・四国支部総会  2009年 

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  • 微生物試験法 細菌一般試験法 免疫学的検査法

    日本薬学会第129年会  2009年 

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  • A novel extracellular protease mediating maturation of Vibrio mimicus hemolysin

    日米医学協力研究会 コレラ・細菌性腸管感染症専門部会 日本側総会  2009年 

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  • 腸炎ビブリオのシデロフォアvibrioferrinを介する鉄獲得系と病原性

    第48回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会  2009年 

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  • ビブリオ・バルニフィカスの金属プロテアーゼ遺伝子に関する研究

    第48回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会  2009年 

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  • ビブリオ・バルニフィカスの増殖と金属ポルフィリン

    日本食品微生物学会30周年記念学術総会  2009年 

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  • ビブリオ・バルニフィカス金属プロテアーゼの無細胞系での合成

    第48回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会  2009年 

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  • ヒト抗菌ペプチドdefensinsのEnterococcus faecalisの生存に対する影響

    第29回日本食品微生物学会学術総会  2008年 

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  • ビブリオ・バルニフィカスの溶血毒素(hemolysin)の活性に必要なアミノ酸残基の解析

    日本薬学会第128年会  2008年 

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  • 新規抗菌薬の開発を指向したヘム鉄獲得系の解析

    日本防菌防黴学会第35回年次大会  2008年 

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  • Vibrio vulnificus溶血毒素の活性発現におけるC末端アミノ酸残基の効果

    第61回日本細菌学会中国・四国支部総会  2008年 

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  • Vibrio vulnificus NCIMB 2137株の分泌するセリンプロテアーゼの精製と性状の解析

    第47回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会  2008年 

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  • Modulation of the activity of Vibrio mimicus hemolysin through limited proteolysis by an endogenous metalloprotease

    43rd Joint Conference on Cholera and Other Bacterial Enteric Infections Panel  2008年 

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  • Vibrio mimicusのトランポゾン変異株の単離と解析

    第47回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会  2008年 

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  • Vibrio mimicus溶血毒素の成熟化に関与するプロテアーゼ

    フォーラム2007 衛生薬学・環境トキシコロジー  2007年 

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  • プロテアーゼ遺伝子に基づいたVibrio vulnificusのグループ分け

    第80回日本細菌学会総会  2007年 

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  • Vibrio mimicusの産生する溶血毒は2種類の経路により腸管上皮細胞のClイオン分泌を促進する

    第80回日本細菌学会総会  2007年 

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  • Vibrio mimicusヘモリジンのプロテアーゼによる限定分解と溶血活性の変化

    第80回日本細菌学会総会  2007年 

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  • 食中毒菌のPCR法による迅速同定

    日本薬学会第127年会  2007年 

     詳細を見る

  • Pore formation of Vibrio mimicus hemolysin in lipid bilayers

    107th Annual Meeting of American Society for Microbiology  2007年 

     詳細を見る

  • 菌数測定:最確数(MPN)法

    日本薬学会第127年会  2007年 

     詳細を見る

  • ノロウイルス検査法

    日本薬学会第127年会  2007年 

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  • Vibrio vulnificus臨床分離株のプロテアーゼの多様性

    日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会  2007年 

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  • Vibrio mimicusヘモリジンの金属プロテアーゼによる溶血活性の変化

    第54回毒素シンポジウム  2007年 

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  • The crucial amino acid residues for stability and activity of Vibrio vulnificus hemolysin

    International Symposium of Vibrio vulnificus and Its Infectious Diseases  2006年 

     詳細を見る

  • Vibrio mimicusの病原因子の探索

    第53回毒素シンポジウム  2006年 

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  • Vibrio vulnificus hemolysinの活性発現に重要なアミノ酸残基

    第53回毒素シンポジウム  2006年 

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  • Vibrio vulnificus 溶血毒素の性状を左右するアミノ酸残基

    日本薬学会第126年会  2006年 

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  • 新規抗菌薬の開発を指向したヘム利用系の解析

    日本防菌防黴学会第33回年次大会  2006年 

     詳細を見る

  • Proteolytic inactivation of an enterotoxic hemolysin produced by Vibrio mimicus

    FOOD MICRO 2006  2006年 

     詳細を見る

  • Vibrio cholerae の選択的単離法の開発

    第18回微生物シンポジウム  2006年 

     詳細を見る

  • Virulence factor(s) produced by Vibrio mimicus

    FOOD MICRO 2006  2006年 

     詳細を見る

  • Amino acid residues relevant for hemolytic activity of Vibrio vulnificus

    FOOD MICRO 2006  2006年 

     詳細を見る

  • Control of protease activity by LuxO in Vibrio mimicus: evidence of the presence of active V. harveyi-like quorum sensing network

    41st Joint Conference on Cholera and Other Bacterial Enteric Infections Pannel  2006年 

     詳細を見る

  • Vibrio mimicusヘモリジンの限定分解と活性の変化

    第40回腸炎ビブリオシンポジウム  2006年 

     詳細を見る

  • Vibrio vulnificusのヘム利用系の特異性

    第18回微生物シンポジウム  2006年 

     詳細を見る

  • Vibrio vulnificus E86株のvvp遺伝子: 塩基配列の決定及びグループ分けへの応用

    第44回日本薬学会中国四国支部学術大会  2005年 

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  • Aeromonas sobriaの産生する溶血毒素の下痢発現におけるATPの関与

    第78回日本細菌学会総会  2005年 

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  • ジクロロメタン分解菌Ralstonia metallidurans PD11株の固定化とバイオリアクターへの応用

    日本防菌防黴学会第32回年次大会  2005年 

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  • ベンガル地域で分離されたVibrio choleraeの分子疫学的,病原学的研究

    日本防菌防黴学会第32回年次大会  2005年 

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  • コレラ流行地域で分離されたVibrio choleraeにおける毒素遺伝子の分布

    第52回毒素シンポジウム  2005年 

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  • Quorum-sensing in Vibrio mimicus

    The 11th International Congress of Bacteriology and Applied Microbiology: International Union of Microbiological Societies  2005年 

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  • The new genetic typing of Vibrio vulnificus strains: comparison of nucleotide sequence of the hemolysin gene vvhA

    The 11th International Congress of Bacteriology and Applied Microbiology; International Union of Microbiological Societies  2005年 

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  • 溶血毒素遺伝子(vvhA)によるVibrio vulnificusの遺伝学的型別

    第52回毒素シンポジウム  2005年 

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  • Immobilization of a dichloromethane degrading bacterium Ralstonia metallidurans PD11 for waste treatment

    he 11th International Congress of Bacteriology and Applied Microbiology; International Union of Microbiological Societies  2005年 

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  • 限定分解を受けたVibrio mimicus溶血毒素の活性

    第39回腸炎ビブリオシンポジウム  2005年 

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  • Vibrio vulnificus溶血毒素の成熟過程

    第39回腸炎ビブリオシンポジウム  2005年 

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  • Immobilization of a dichloromethane degrading bacterium Ralstonia metallidurans PD11 for waste treatment

    7th Symposium on Asian Academic Network for Environmental Safety and Waste Management  2005年 

     詳細を見る

  • Vibrio vulnificusのdesRAに基づくグルーピング

    第39回腸炎ビブリオシンポジウム  2005年 

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  • Vibrio parahaemolyticusの二種類のコラゲナーゼの比較研究

    第39回腸炎ビブリオシンポジウム  2005年 

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  • インド・ベンガル地域で単離された Vibrio cholerae臨床株・環境株の 分子生物学的比較研究

    第39回腸炎ビブリオシンポジウム  2005年 

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  • Vibrio vulnificus株間におけるdesferal利用遺伝子desRAの比較

    第44回日本薬学会中国四国支部学術大会  2005年 

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  • Vibrio vulnificus infection and metalloprotease

    The 14th Japan-Korea Joint Meeting of Dermatology  2005年 

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  • 発酵食品テンペから分離されたEnterococcus faecalisの産生するプロテアーゼ

    第26回日本食品微生物学会学術総会  2005年 

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  • ビブリオ・バル二フィカスにおけるクォーラム・センシング調節

    第44回日本薬学会中国四国支部学術大会  2005年 

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  • ビブリオ・バル二フィカスにおける毒素産生のクォーラム・センシング調節について

    第78回日本細菌学会総会  2005年 

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  • ベンガル地域で分離されたVibrio cholerae分子疫学的,病原学的研究

    第78回日本細菌学会総会  2005年 

     詳細を見る

  • Molecular epidemiological study of Vibrio cholerae in Bengal region

    Fortieth anniversary United States-Japan cooperative medical science program  2004年 

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  • ジクロロメタン分解菌 Ralstonia metallidurans PD11 株の分解特性及びバイオリアクターへの応用

    日本薬学会第124年会  2004年 

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  • 1,3-dichrolo-2-propanol 分解菌 Arthrobavter sp. PY1 株の分解特性

    日本薬学会第124年会  2004年 

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  • Aeromonas sobria の産生する溶血毒素の下痢発症機序の解析

    第77回日本細菌学会総会  2004年 

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  • Vibrio vulnificus のプロテアーゼ産生における AI-2 依存調節系の重要性

    第77回日本細菌学会総会  2004年 

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  • ベンガル地域におけるコレラ関連細菌の分子疫学的研究

    日本薬学会第124年会  2004年 

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  • 衛生試験法における赤痢菌とコレラ菌の検出法に関する提案

    日本薬学会第124年会  2004年 

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  • ベンガル地域で分離されたナグビブリオの分子疫学的および病原学的研究

    日本防菌防黴学会第31回年次大会  2004年 

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  • ジクロロメタン分解菌 Ralstonia metallidurans PD11 株の分解特性及びバイオリアクターへの応用

    日本防菌防黴学会第31回年次大会  2004年 

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  • 溶血毒素遺伝子による Vibrio vulnificus の型別

    第77回日本細菌学会総会  2004年 

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  • Vibrio vulnificus 溶血毒素(VVH)の分子生物学的研究

    第57回日本細菌学会中国・四国支部総会  2004年 

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  • The cytotoxin-hemolysin genes of human and eel pathogenic Vibrio vulnificus strains: comparison of nucleotide sequences and application to the genetic typing

    The 7th Kora-Japan international symposium on microbiology  2004年 

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  • 1,3-dichloro-2-propanol 分解菌 Arthrobacter sp. PY1 株の分解特性

    日本防菌防黴学会第31回年次大会  2004年 

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  • Growth-phase dependence of protease production by Vibrio vulnificus, an etiological agent of fatal food-borne disease

    The 19th international ICFMH symposium  2004年 

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  • ビブリオ・バルニフィカス溶血毒素オペロン(vvhBA)の機能解析

    第43回日本薬学会中国四国支部学術大会  2004年 

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  • Molecular characterization of a multidrug-resistant strain of enteroinvasive Escherichia coli O164 isolated in Japan

    The 7th Korea-Japan international symposium on microbiology  2004年 

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  • Arthrobacter sp. PY1 株固定化担体を用いた 1,3-dichloro-2-propanol の分解

    第43回日本薬学会中国四国支部学術総会  2004年 

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  • Vibrio vulnificus の金属プロテアーゼ産生における AI-2 依存性調節系の関与

    第38回腸炎ビブリオシンポジウム  2004年 

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  • Presence of Vibrio harveyi signaling sysyem-2 like quorum sensing system in V. mimicus

    Fortieth anniversary United States-Japan cooperative medical science program  2004年 

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  • ビブリオ・バルニフィカスにおける毒素産生のクォーラム・センシング調節について

    第43回日本薬学会中国四国支部学術大会  2004年 

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  • Vibrio mimicus が保有するクォーラムセンシング調節遺伝子とシグナル分子

    第38回腸炎ビブリオシンポジウム  2004年 

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  • ジクロメタン分解菌Ralstonia metallidurans PD11株:その性質とジクロ分解処理への応用について

    日本薬学会第123年会  2003年 

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  • ビブリオ・バルニフィカス金属プロテアーゼにおけるクォーラム・センシング調節

    日本薬学会第123年会  2003年 

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  • ビブリオ・バルニフィカス金属プロテアーゼの産生におけるクォーラム・センシング調節

    日本薬学会第123年会  2003年 

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  • 脂肪族ハロアルコール分解菌の探索とその分解活性

    日本薬学会第123年会  2003年 

     詳細を見る

  • Identification and characterization of class 1 integron-mediated resistance among Salmonella strains

    第24回日本食品微生物学会学術総会  2003年 

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  • Aeromonas sobriaの分泌する溶血毒とその類似毒素の下痢誘発機構の比較研究

    第76回日本細菌学会総会  2003年 

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  • ジクロロメタン分解菌Ralostonia metallidurans PD11株 -その性状とジクロロメタン分解処理への応用-

    日本薬学会第123年会  2003年 

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  • 脂肪属ハロアルコール分解菌の探索とその分解活性

    日本薬学会第123年会  2003年 

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  • Aeromonas sobriaの産生する溶血毒素の下痢発現機構の解析

    第76回日本細菌学会総会  2003年 

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  • Vibrio parahaemolyticusの産生するセリンプロテアーゼ -その病原因子としての可能性-

    第76回日本細菌学会総会  2003年 

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  • Arthrobacter sp. strain PY1による1.3-dichloro-2-propanolの分解

    2003年度日本防菌防黴学会若手の会  2003年 

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  • ベンガル地域におけるVibrio cholerae及び関連細菌の分子疫学的研究

    第76回日本細菌学会総会  2003年 

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  • 脂肪族ハロアルコール分解菌(PY1株)の分解活性

    日本防菌防黴学会第30回年次大会  2003年 

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  • ジクロロメタン分解菌Ralostonia sp. PD11株の応用に向けた基礎的研究及び遺伝学的研究

    日本防菌防黴学会第30回年次大会  2003年 

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  • 非定型Vibrio choleraeおよびVibrio mimicusのsucrose利用能に関する分子生物学的解析

    第76回日本細菌学会総会  2003年 

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  • ベンガル地域におけるVibrio choleraeの分離及び遺伝学的解析

    日本防菌防黴学会第30回年次大会  2003年 

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  • 溶血毒素遺伝子によるVibrio vulnificusのtyping

    第24回日本食品微生物学会学術総会  2003年 

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  • ジクロロメタン分解菌Ralostonia metallidurans PD11株 -バイオリアクター応用に関する検討および遺伝学的検討について-

    フォーラム2003:衛生薬学・環境トキシコロジー  2003年 

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  • 腸炎ビブリオセリンプロテアーゼの血管透過性亢進活性

    第50回毒素シンポジウム  2003年 

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  • 東南アジア伝統的発酵食品テンペから分離した乳酸菌Enterococcus faecalis TH10酸性物質の抗菌性について

    第24回日本食品微生物学会学術総会  2003年 

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  • ベンガル地域におけるコレラ菌およびナグビブリオの分子疫学的研究

    第37回腸炎ビブリオシンポジウム  2003年 

     詳細を見る

  • 人喰い菌ビブリオ・バルニフィカスの毒素産生調節システム:細胞間コミュニケーション

    第42回日本薬学会中国四国支部学術大会  2003年 

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  • ジクロロメタン分解菌Ralstonia metallidurans PD11株の分解能力の検討

    第42回日本薬学会中国四国支部学術総会  2003年 

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  • ビブリオ・バルニフィカスの溶血毒素遺伝子の塩基配列の決定と型別への応用

    第42回日本薬学会中国四国支部学術総会  2003年 

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  • Vibrio vulnificusにおける金属プロテアーゼの産生調節機構

    第36回腸炎ビブリオシンポジウム  2002年 

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  • 腸炎ビブリオの産生するプロテアーゼに関する研究

    第36回腸炎ビブリオシンポジウム  2002年 

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  • ビブリオ金属プロテアーゼの病原性:病原菌と非病原菌の金属プロテアーゼの比較

    第75回日本細菌学会総会  2002年 

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  • Vibrio vulnificus臨床分離株はマクロファージにアポトーシスを誘導する

    第75回日本細菌学会総会  2002年 

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  • 非定型Vibrio choleraeにおけるsucrose利用能欠損原因の解析

    第75回日本細菌学会総会  2002年 

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  • 腸炎ビブリオにおけるvibrioferrinを介する鉄獲得系遺伝子群の解析

    第75回日本細菌学会総会  2002年 

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  • Vibrio vulnificusにおける外因性シデロフォアによる外膜レセプターの誘導と遺伝子解析

    第75回日本細菌学会総会  2002年 

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  • ジクロメタン分解菌の分離とその分解能力の検討

    日本防菌防黴学会第29回年次大会  2002年 

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  • Aeromonas sobriaの産生する溶血毒素の下痢発現機構

    第55回日本細菌学会中国四国支部総会  2002年 

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  • Acinetobacter baumanniiにおけるacinetobactinを介する鉄獲得系遺伝子群の解析

    第75回日本細菌学会総会  2002年 

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  • Vibrio mimicus分離株における病原遺伝子の分布

    日本防菌防黴学会第29回年次大会  2002年 

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  • Vibrio vulnificusの亜鉛金属プロテアーゼ産生におけるクォーラムセンシングの関与

    第55回日本細菌学会中国四国支部総会  2002年 

     詳細を見る

  • Vibrio parahaemolyticusの産生するセリンプロテアーゼ:その病原因子としての可能性

    第41回日本薬学会中国四国支部学術大会  2002年 

     詳細を見る

  • Vibrio vulnificusにおけるaerobactinによる当該外膜レセプターの発現誘導機構

    第55回日本細菌学会中国四国支部総会  2002年 

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  • Vibrio mimicusにおけるaerobactinオペロンの発現調節と遺伝子破壊による機能解析

    第55回日本細菌学会中国四国支部総会  2002年 

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  • Acinetobacter baumanniiにおけるacnetobactin(鉄輸送キレーター)生合成遺伝子群の転写制御および生合成経路について

    第41回日本薬学会中国四国支部学術大会  2002年 

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  • 腸炎ビブリオにおけるferric aerobactinに対する外膜受容体遺伝子のクローニングと解析

    第41回日本薬学会中国四国支部学術大会  2002年 

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  • 非定型Vibrio choleraeおよびVibrio mimicusのsucrose利用能欠損原因の解析

    第41回日本薬学会中国四国支部学術大会  2002年 

     詳細を見る

  • An enterotoxic hemolysin produced by Vibrio mimicus

    International symposium on toxins and natural products  2002年 

     詳細を見る

  • Functional domains of a zinc metalloprotease from Vibrio vulnificus

    International symposium on toxins and natural products  2002年 

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  • 脂肪族ハロアルコールの微生物分解

    第41回日本薬学会中国四国支部学術大会  2002年 

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  • ジクロメタン分解菌の分離とその分解能力の検討

    第41回日本薬学会中国四国支部学術大会  2002年 

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  • Vibrio属菌におけるaerobactin利用系遺伝子群の多様性について

    第36回腸炎ビブリオシンポジウム  2002年 

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  • Acinetobacter Baumanniiにおける鉄獲得系遺伝子の解析

    第40回日本薬学会中国・四国支部学術大会  2001年 

     詳細を見る

  • 病原ビブリオの鉄獲得機構

    第74回日本細菌学会総会  2001年 

     詳細を見る

  • 腸炎ビブリオVBNC菌の再生過程の解析

    第74回日本細菌学会総会  2001年 

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  • Vibrio vulnificusプロテアーゼの赤血球凝集活性

    第74回日本細菌学会総会  2001年 

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  • 腸炎ビブリオは3種類のferric siderophoreに対する外膜レセプターを発現する

    第74回日本細菌学会総会  2001年 

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  • Vibrio mimicusにおけるaerobactinオペロンの遺伝子解析

    第74回日本細菌学会総会  2001年 

     詳細を見る

  • Vibrio mimicus臨床分離株の病原因子遺伝子

    第74回日本細菌学会総会  2001年 

     詳細を見る

  • Pathogenic factors of Vibrio vulnificus

    11th World Congress of Food Science and Technology  2001年 

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  • 腸炎ビブリオのirgAB遺伝子:クローニング,発現調節とIrgAの菌体外分泌について

    第74回日本細菌学会総会  2001年 

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  • Acinetobacter baumanniiの鉄製御遺伝子の単離と解析

    第74回日本細菌学会総会  2001年 

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  • 病原細菌Vibrio vulnificusのヘム獲得機構の特異性

    第11回金属の関与する生体関連反応シンポジウム  2001年 

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  • 腸炎ビブリオにおける鉄レギュロン関連遺伝子の解析

    第11回金属の関与する生体関連反応シンポジウム  2001年 

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  • 海水中からの腸炎ビブリオの検出方法に関する研究

    第28回日本防菌防黴学会  2001年 

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  • 環境分離菌が産生するチトクロムP450による内分泌撹乱化学物質の分解

    第28回日本防菌防黴学会  2001年 

     詳細を見る

  • 限定分解を受けたVibrio mimicus溶血毒素の生物活性

    第48回毒素シンポジウム  2001年 

     詳細を見る

  • Vibrio choleraeおよびVibrio mimicusのsucrose利用能に関する遺伝学的解析

    第54回日本細菌学会中国・四国支部総会  2001年 

     詳細を見る

  • Biological activities of the proteolyzed derivative from Vibrio mimicus hemolysin

    10th European Workshop Conference on Bacterial Pritein Toxins  2001年 

     詳細を見る

  • Vibrio parahaemolyticusの鉄獲得系:1. vibrioferin生合成遺伝子の解析

    第54回日本細菌学会中国・四国支部総会  2001年 

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  • Vibrio parahaemolyticusの鉄獲得系:2. ferric vibrioferin輸送遺伝子の解析

    第54回日本細菌学会中国・四国支部総会  2001年 

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  • ヒト非病原性ビブリオ属細菌の分泌する金属プロテアーゼに関する研究

    第54回日本細菌学会中国・四国支部総会  2001年 

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  • Vibrio vulnificusにおけるferric aerobactin receptor遺伝子の解析

    第54回日本細菌学会中国・四国支部総会  2001年 

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  • 腸炎ビブリオが産生するプロテアーゼに関する研究

    第35回腸炎ビブリオシンポジウム  2001年 

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  • ヒト血清中に認められた細菌DNAの解析

    第54回日本細菌学会中国・四国支部総会  2001年 

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  • 腸炎ビブリオの鉄獲得系遺伝子の解析

    第35回腸炎ビブリオシンポジウム  2001年 

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  • Vibrio vulnificusプロテアーゼの赤血球凝集作用

    第40回日本薬学会中国・四国支部学術大会  2001年 

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  • 芳香族ハロアルコール分解菌の探索とその分解活性

    第40回日本薬学会中国・四国支部学術大会  2001年 

     詳細を見る

  • 非定型コレラ菌および類縁菌におけるスクロース利用能欠損の解析

    第40回日本薬学会中国・四国支部学術大会  2001年 

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  • Vibrio mimicusにおける病原遺伝子の分布

    第40回日本薬学会中国・四国支部学術大会  2001年 

     詳細を見る

  • 環境分離菌が産生するチトクロムP450による内分泌撹乱化学物質の分解

    第40回日本薬学会中国・四国支部学術大会  2001年 

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  • Vibrio属菌フェリチン遺伝子(ftn)のクローニングと解析

    第40回日本薬学会中国・四国支部学術大会  2001年 

     詳細を見る

  • Pathological actions of Vibrio vulnificus metalloprotease on the capillaries

    2nd Workshop on Natural Toxins  2000年 

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  • Vibrio fluvialisの産生する金属プロテアーゼ

    第73回日本細菌学会総会  2000年 

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  • Hemorrhagic damage caused by Vibrio vulnificus metalloprotease

    The Millennium for Microbiology Meeting  2000年 

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  • Identification and characterization of a gene encoding the outer membrane receptor for heme in Vibrio mimicus

    The Millennium for Microbiology Meeting  2000年 

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  • 腸炎ビブリオferric vibrioferrinレセプターのクローニングと解析

    第73回日本細菌学会総会  2000年 

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  • Vibrio mimicus腸管付着因子としての主要外膜タンパク質の菌株間における多様性の解析

    第73回日本細菌学会総会  2000年 

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  • Vibrio mimicusヘモリジン(VMH)の作用に及ぼすメンブレンポテンシャルの役割

    第47回毒素シンポジウム  2000年 

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  • 岡山県児島湾において高頻度に分離されたtdh遺伝子保有腸炎ビブリオにおける病原因子の解析

    第34回腸炎ビブリオシンポジウム  2000年 

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  • Isolation and characterization of a novel cytochrome P450 from environmental bacteria

    The Millennium for Microbiology Meeting  2000年 

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  • ガラガラヘビ(Crotalus atrox)の毒液に含まれるムスカリン性アセチルコリン受容体阻害因子

    第47回毒素シンポジウム  2000年 

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  • 腸炎ビブリオの鉄製御外膜蛋白をコードする遺伝子(pfuA,pvuA,pauA)のクローニングと解析

    第34回腸炎ビブリオシンポジウム  2000年 

     詳細を見る

  • 腸炎ビブリオpvuA(ferric vibrioferrin receptor)遺伝子のクローニングと解析

    第53回日本細菌学会中国・四国支部総会  2000年 

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  • 海水より分離した腸炎ビブリオにおいて高頻度に検出されたtdhおよびtrh遺伝子の解析

    第53回日本細菌学会中国・四国支部総会  2000年 

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  • Acinetobacter baumanniiにおける鉄制御遺伝子群の単離と解析

    第39回日本薬学会中国・四国支部学術大会  2000年 

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  • Vibrio mimicusヘムレセプター遺伝子(mhuA)のクローニングと転写調節について

    第39回日本薬学会中国・四国支部学術大会  2000年 

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  • Vibrio vulnificusヘモリジン(VVH)の構造と活性

    第53回日本細菌学会中国・四国支部総会  2000年 

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  • 環境分離株の産生するチトクロムP450の精製と性質解析

    第39回日本薬学会中国・四国支部学術大会  2000年 

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  • Vibrio vulnificusプロテアーゼの赤血球凝集作用

    第39回日本薬学会中国・四国支部学術大会  2000年 

     詳細を見る

▼全件表示

受賞

  • 日本薬学会 中国四国支部奨励賞

    1999年  

     詳細を見る

    受賞国:日本国

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  • 日本細菌学会 黒屋奨学賞

    1999年  

     詳細を見る

    受賞国:日本国

    researchmap

 

担当授業科目

  • 公衆衛生学1 (2021年度) 第1学期  - 火1,火2

  • 公衆衛生学2 (2021年度) 第2学期  - 火1,火2

  • 微生物学 (2021年度) 3・4学期  - 金3,金4

  • 微生物学 (2021年度) 3・4学期  - 金3,金4

  • 環境微生物化学 (2021年度) 特別  - その他

  • 生命科学2 (2021年度) 後期  - その他

  • 衛生微生物化学I(演習・実習) (2021年度) 特別  - その他

  • 衛生微生物化学I(講義・演習) (2021年度) 特別  - その他

  • 衛生微生物化学II(演習・実習) (2021年度) 特別  - その他

  • 衛生微生物化学II(講義・演習) (2021年度) 特別  - その他

  • 衛生薬学実習 (2021年度) 第3学期  - その他6~9

  • 衛生薬学実習 (2021年度) 第3学期  - その他6~9

  • 衛生薬学3 (2021年度) 第1学期  - 金1,金2

  • 衛生薬学3 (2021年度) 第1学期  - 金1,金2

  • 衛生薬学4 (2021年度) 第2学期  - 金1,金2

  • 衛生薬学4 (2021年度) 第2学期  - 金1,金2

  • 衛生薬学6 (2021年度) 第4学期  - 水3,水4,水5

  • 衛生薬学6 (2021年度) 第4学期  - 水3,水4,水5

  • 微生物学 (2020年度) 3・4学期  - 金3,金4

  • 微生物学 (2020年度) 3・4学期  - 金3,金4

  • 微生物学 (2020年度) 3・4学期  - 金3,金4

  • 環境微生物化学 (2020年度) 特別  - その他

  • 生命科学2 (2020年度) 特別  - その他

  • 衛生微生物化学I(演習・実習) (2020年度) 特別  - その他

  • 衛生微生物化学I(講義・演習) (2020年度) 特別  - その他

  • 衛生微生物化学II(演習・実習) (2020年度) 特別  - その他

  • 衛生微生物化学II(講義・演習) (2020年度) 特別  - その他

  • 衛生薬学実習 (2020年度) 特別  - その他

  • 衛生薬学実習 (2020年度) 特別  - その他

  • 衛生薬学3 (2020年度) 第1学期  - 金1,金2

  • 衛生薬学3 (2020年度) 第1学期  - 金1,金2

  • 衛生薬学4 (2020年度) 第2学期  - 金1,金2

  • 衛生薬学4 (2020年度) 第2学期  - 金1,金2

  • 衛生薬学6 (2020年度) 第4学期  - 水3,水4

  • 衛生薬学6 (2020年度) 第4学期  - 水3,水4

  • 衛生薬学II (2020年度) 1~4学期  - [第1学期]金1,金2, [第2学期]水3,水4, [第3学期]水3,水4, [第4学期]水3,水4

▼全件表示