2022/01/27 更新

写真a

タケイ コウジ
竹居 孝二
TAKEI Kohji
所属
医歯薬学域 教授
職名
教授
外部リンク

学位

  • 歯学博士 ( 東京歯科大学 )

研究キーワード

  • 細胞内小胞輸送

  • 細胞生物

  • cytoskeleton

  • membrane traffic

  • cell biology

  • 細胞骨格

研究分野

  • ライフサイエンス / 細胞生物学

学歴

  • 東京歯科大学   Graduate School, Division of Dental Research  

    - 1989年

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  • 東京歯科大学    

    - 1989年

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    国名: 日本国

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  • 東京歯科大学   Faculty of Dentistry   Dentistry

    - 1985年

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  • 東京歯科大学   College of Dentistry  

    - 1985年

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    国名: 日本国

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経歴

  • - 岡山大学医歯薬学総合研究科 教授

    2005年

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  • - Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2005年

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  • 岡山大学医歯学総合研究科 未設定

    2001年 - 2005年

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  • Research Specialist II,Howard Hughes Medical Institute

    1995年 - 1999年

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  • ハワードヒューズ 医学財団 リサーチスペシャリスト

    1995年 - 1999年

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  • Associate Research Scientist,Yale University, School of Medicine

    1993年 - 1999年

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  • エール大学医学部 アソシエートリサーチサイエンティスト

    1993年 - 1999年

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  • ハワードヒューズ 医学財団 ポストドクトラルアソシエート

    1992年 - 1994年

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  • Postdoctoral Associate,Howard Hughes Medical Institute

    1992年 - 1994年

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  • postdoctoral fellow,Yale Unversity, School of Medicine

    1988年 - 1993年

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  • エール大学医学部 ポストドクトラルフェロー

    1988年 - 1993年

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▼全件表示

所属学協会

 

論文

  • JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes

    Ayuko Sakane, Taka-aki Yano, Takayuki Uchihashi, Kazuki Horikawa, Yusuke Hara, Issei Imoto, Shusaku Kurisu, Hiroshi Yamada, Kohji Takei, Takuya Sasaki

    Communications Biology   4 ( 1 )   2021年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    <title>Abstract</title>Elongated tubular endosomes play essential roles in diverse cellular functions. Multiple molecules have been implicated in tubulation of recycling endosomes, but the mechanism of endosomal tubule biogenesis has remained unclear. In this study, we found that JRAB/MICAL-L2 induces endosomal tubulation via activated Rab8A. In association with Rab8A, JRAB/MICAL-L2 adopts its closed form, which functions in the tubulation of recycling endosomes. Moreover, JRAB/MICAL-L2 induces liquid–liquid phase separation, initiating the formation of tubular recycling endosomes upon overexpression. Between its N-terminal and C-terminal globular domains, JRAB/MICAL-L2 contains an intrinsically disordered region, which contributes to the formation of JRAB/MICAL-L2 condensates. Based on our findings, we propose that JRAB/MICAL-L2 plays two sequential roles in the biogenesis of tubular recycling endosomes: first, JRAB/MICAL-L2 organizes phase separation, and then the closed form of JRAB/MICAL-L2 formed by interaction with Rab8A promotes endosomal tubulation.

    DOI: 10.1038/s42003-021-02080-7

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    その他リンク: http://www.nature.com/articles/s42003-021-02080-7

  • Imaging-based evaluation of pathogenicity by novel DNM2 variants associated with centronuclear myopathy. 国際誌

    Kenshiro Fujise, Mariko Okubo, Tadashi Abe, Hiroshi Yamada, Kohji Takei, Ichizo Nishino, Tetsuya Takeda, Satoru Noguchi

    Human mutation   2021年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A centronuclear myopathy (CNM) is a group of inherited congenital diseases showing clinically progressive muscle weakness associated with the presence of centralized myonuclei, diagnosed by genetic testing and muscle biopsy. The gene encoding dynamin 2, DNM2, has been identified as a causative gene for an autosomal dominant form of CNM. However, the information of a DNM2 variant alone is not always sufficient to gain a definitive diagnosis as the pathogenicity of many gene variants is currently unknown. In this study, we identified five novel DNM2 variants in our cohort. To establish the pathogenicity of these variants without using clinicopathological information, we used a simple in cellulo imaging-based assay for T-tubule-like structures to provide quantitative data that enable objective determination of pathogenicity by novel DNM2 variants. With this assay, we demonstrated that the phenotypes induced by mutant dynamin 2 in cellulo are well correlated with biochemical gain-of-function features of mutant dynamin 2 as well as the clinicopathological phenotypes of each patient. Our approach of combining an in cellulo assay with clinical information of the patients also explains the course of a disease progression by the pathogenesis of each variant in DNM2-associated CNM.

    DOI: 10.1002/humu.24307

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  • Dynamin 2 and BAR domain protein pacsin 2 cooperatively regulate formation and maturation of podosomes

    Jianzhen Li, Kenshiro Fujise, Haymar Wint, Yosuke Senju, Shiro Suetsugu, Hiroshi Yamada, Kohji Takei, Tetsuya Takeda

    Biochemical and Biophysical Research Communications   571   145 - 151   2021年9月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.bbrc.2021.07.041

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  • Mutant BIN1-Dynamin 2 complexes dysregulate membrane remodeling in the pathogenesis of centronuclear myopathy. 国際誌

    Kenshiro Fujise, Mariko Okubo, Tadashi Abe, Hiroshi Yamada, Ichizo Nishino, Satoru Noguchi, Kohji Takei, Tetsuya Takeda

    The Journal of biological chemistry   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Membrane remodeling is required for dynamic cellular processes such as cell division, polarization and motility. BAR domain proteins and dynamins are key molecules in membrane remodeling that work together for membrane deformation and fission. In striated muscles, sarcolemmal invaginations termed T-tubules are required for excitation-contraction coupling. BIN1 and DNM2, which encode a BAR domain protein BIN1 and dynamin 2, respectively, have been reported to be causative genes of centronuclear myopathy (CNM), a hereditary degenerative disease of skeletal muscle, and deformation of T-tubules is often observed in the CNM patients. However, it remains unclear how BIN1 and dynamin 2 are implicated in T-tubule biogenesis, and how mutations in these molecules cause CNM to develop.Here, using an in cellulo reconstitution assay, we demonstrate that dynamin 2 is required for stabilization of membranous structures equivalent to T-tubules. GTPase activity of wild type dynamin 2 is suppressed through interaction with BIN1, whereas that of the disease-associated mutant dynamin 2 remains active due to lack of the BIN1-mediated regulation thus causing aberrant membrane remodeling. Finally, we show that in cellulo aberrant membrane remodeling by mutant dynamin 2 variants is correlated with their enhanced membrane fission activities, and the results can explain severity of the symptoms in patients. Thus, this study provides molecular insights into dysregulated membrane remodeling triggering the pathogenesis of DNM2-related centronuclear myopathy.

    DOI: 10.1074/jbc.RA120.015184

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  • Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes

    The Mon La, Hiromi Tachibana, Shun‐Ai Li, Tadashi Abe, Sayaka Seiriki, Hikaru Nagaoka, Eizo Takashima, Tetsuya Takeda, Daisuke Ogawa, Shin‐ichi Makino, Katsuhiko Asanuma, Masami Watanabe, Xuefei Tian, Shuta Ishibe, Ayuko Sakane, Takuya Sasaki, Jun Wada, Kohji Takei, Hiroshi Yamada

    The FASEB Journal   2020年10月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1096/fj.202001240rr

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1096/fj.202001240RR

  • Internalization of AMPA-type Glutamate Receptor in the MIN6 Pancreatic β-cell Line. 査読

    The Mon La, Hiroshi Yamada, Sayaka Seiriki, Shun-Ai Li, Kenshiro Fujise, Natsuho Katsumi, Tadashi Abe, Masami Watanabe, Kohji Takei

    Cell structure and function   45 ( 2 )   121 - 130   2020年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The activity of AMPA-type glutamate receptor is involved in insulin release from pancreatic β-cells. However, the mechanism and dynamics that underlie AMPA receptor-mediated insulin release in β-cells is largely unknown. Here, we show that AMPA induces internalization of glutamate receptor 2/3 (GluR2/3), AMPA receptor subtype, in the mouse β-cell line MIN6. Immunofluorescence experiments showed that GluR2/3 appeared as fine dots that were distributed throughout MIN6 cells. Intracellular GluR2/3 co-localized with AP2 and clathrin, markers for clathrin-coated pits and vesicles. Immunoelectron microscopy revealed that GluR2/3 was also localized at plasma membrane. Surface biotinylation and immunofluorescence measurements showed that addition of AMPA caused an approximate 1.8-fold increase in GluR2/3 internalization under low-glucose conditions. Furthermore, internalized GluR2 largely co-localized with EEA1, an early endosome marker. In addition, GluR2/3 co-immunoprecipitated with cortactin, a F-actin binding protein. Depletion of cortactin by RNAi in MIN6 cells altered the intracellular distribution of GluR2/3, suggesting that cortactin is involved in internalization of GluR2/3 in MIN6 cells. Taken together, our results suggest that pancreatic β-cells adjust the amount of AMPA-type GluR2/3 on the cell surface to regulate the receptive capability of the cell for glutamate.Key words: endocytosis, GluR2, AMPA, cortactin, MIN6.

    DOI: 10.1247/csf.20020

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  • [Corrigendum]Phosphorylation of cortactin by cyclin-dependent kinase 5 modulates actin bundling by the dynamin 1-cortactin ring-like complex and formation of filopodia and lamellipodia in NG108-15 glioma-derived cells. 国際誌

    Tadashi Abe, The Mon La, Yuuzi Miyagaki, Eri Oya, Fan-Yan Wei, Kento Sumida, Kenshiro Fujise, Tetsuya Takeda, Kazuhito Tomizawa, Kohji Takei, Hiroshi Yamada

    International journal of oncology   56 ( 3 )   859 - 859   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Subsequently to the publication of the above article, the authors have realized that the second‑listed author, The Mon La, had not been properly credited as one of the co‑writers of the paper. Therefore, the Authors' Contributions of the Declarations section of the article should have read as follows: Authors' contributions HY, KTa and TML designed the research and wrote the paper. HY, TA, YM, EO and TT performed mutant protein construction, protein purification and actin bundling experiments. TA and YM performed electron microscopy. EO, TML, KS and KF performed immunofluorescent microscopy, cell migration assay and analyzed data. FYW and KTo identified phosphorylation sites by MALDI‑MS. All authors read and approved the final manuscript. The authors apologize to the readership of the Journal for the misinformation in this regard, and for any inconvenience caused. [the original article was published in International Journal of Oncology 54: 550‑558, 2019; DOI: 10.3892/ijo.2018.4663].

    DOI: 10.3892/ijo.2020.4962

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  • Initial phospholipid-dependent Irgb6 targeting to Toxoplasma gondii vacuoles mediates host defense. 査読 国際誌

    Youngae Lee, Hiroshi Yamada, Ariel Pradipta, Ji Su Ma, Masaaki Okamoto, Hikaru Nagaoka, Eizo Takashima, Daron M Standley, Miwa Sasai, Kohji Takei, Masahiro Yamamoto

    Life science alliance   3 ( 1 )   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Toxoplasma gondii is an obligate intracellular protozoan parasite capable of infecting warm-blooded animals by ingestion. The organism enters host cells and resides in the cytoplasm in a membrane-bound parasitophorous vacuole (PV). Inducing an interferon response enables IFN-γ-inducible immunity-related GTPase (IRG protein) to accumulate on the PV and to restrict parasite growth. However, little is known about the mechanisms by which IRG proteins recognize and destroy T. gondii PV. We characterized the role of IRG protein Irgb6 in the cell-autonomous response against T. gondii, which involves vacuole ubiquitination and breakdown. We show that Irgb6 is capable of binding a specific phospholipid on the PV membrane. Furthermore, the absence of Irgb6 causes reduced targeting of other effector IRG proteins to the PV. This suggests that Irgb6 has a role as a pioneer in the process by which multiple IRG proteins access the PV. Irgb6-deficient mice are highly susceptible to infection by a strain of T. gondii avirulent in wild-type mice.

    DOI: 10.26508/lsa.201900549

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  • ATP6AP2 variant impairs CNS development and neuronal survival to cause fulminant neurodegeneration. 査読 国際誌

    Takuo Hirose, Alfredo Cabrera-Socorro, David Chitayat, Thomas Lemonnier, Olivier Féraud, Carmen Cifuentes-Diaz, Nicolas Gervasi, Cedric Mombereau, Tanay Ghosh, Loredana Stoica, Jeanne d'Arc Al Bacha, Hiroshi Yamada, Marcel A Lauterbach, Marc Guillon, Kiriko Kaneko, Joy W Norris, Komudi Siriwardena, Susan Blasér, Jérémie Teillon, Roberto Mendoza-Londono, Marion Russeau, Julien Hadoux, Sadayoshi Ito, Pierre Corvol, Maria G Matheus, Kenton R Holden, Kohji Takei, Valentina Emiliani, Annelise Bennaceur-Griscelli, Charles E Schwartz, Genevieve Nguyen, Matthias Groszer

    The Journal of clinical investigation   129 ( 5 )   2145 - 2162   2019年4月

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    記述言語:英語  

    Vacuolar H+-ATPase-dependent (V-ATPase-dependent) functions are critical for neural proteostasis and are involved in neurodegeneration and brain tumorigenesis. We identified a patient with fulminant neurodegeneration of the developing brain carrying a de novo splice site variant in ATP6AP2 encoding an accessory protein of the V-ATPase. Functional studies of induced pluripotent stem cell-derived (iPSC-derived) neurons from this patient revealed reduced spontaneous activity and severe deficiency in lysosomal acidification and protein degradation leading to neuronal cell death. These deficiencies could be rescued by expression of full-length ATP6AP2. Conditional deletion of Atp6ap2 in developing mouse brain impaired V-ATPase-dependent functions, causing impaired neural stem cell self-renewal, premature neuronal differentiation, and apoptosis resulting in degeneration of nearly the entire cortex. In vitro studies revealed that ATP6AP2 deficiency decreases V-ATPase membrane assembly and increases endosomal-lysosomal fusion. We conclude that ATP6AP2 is a key mediator of V-ATPase-dependent signaling and protein degradation in the developing human central nervous system.

    DOI: 10.1172/JCI79990

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  • Phosphorylation of cortactin by cyclin-dependent kinase 5 modulates actin bundling by the dynamin 1-cortactin ring-like complex and formation of filopodia and lamellipodia in NG108-15 glioma-derived cells. 査読

    Abe T, La TM, Miyagaki Y, Oya E, Wei FY, Sumida K, Fujise K, Takeda T, Tomizawa K, Takei K, Yamada H

    International journal of oncology   54 ( 2 )   550 - 558   2019年2月

  • Dynamic clustering of dynamin-amphiphysin helices regulates membrane constriction and fission coupled with GTP hydrolysis. 査読

    Takeda T, Kozai T, Yang H, Ishikuro D, Seyama K, Kumagai Y, Abe T, Yamada H, Uchihashi T, Ando T, Takei K

    eLife   7   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.7554/eLife.30246

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  • Phosphatidic acid induces EHD3-containing membrane tubulation and is required for receptor recycling 査読

    Yuji Henmi, Natsuko Oe, Nozomu Kono, Tomohiko Taguchi, Kohji Takei, Kenji Tanabe

    Experimental Cell Research   342 ( 1 )   1 - 10   2016年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier {BV}  

    DOI: 10.1016/j.yexcr.2016.02.011

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  • Inhibition of SGLT2 alleviates diabetic nephropathy by suppressing high glucose-induced oxidative stress in type 1 diabetic mice 査読

    Hatanaka T, Ogawa D, Tachibana H, Eguchi J, Inoue T, Yamada H, Takei K, Makino H, Wada J

    Pharmacol Res Perspect   4 ( 4 )   e00239   2016年

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  • Long-Term Treatment with the Sodium Glucose Cotransporter 2 Inhibitor, Dapagliflozin, Ameliorates Glucose Homeostasis and Diabetic Nephropathy in db/db Mice 査読

    Naoto Terami, Daisuke Ogawa, Hiromi Tachibana, Takashi Hatanaka, Jun Wada, Atsuko Nakatsuka, Jun Eguchi, Chikage Sato Horiguchi, Naoko Nishii, Hiroshi Yamada, Kohji Takei, Hirofumi Makino

    PLOS ONE   9 ( 6 )   e100777   2014年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Inhibition of sodium glucose cotransporter 2 (SGLT2) has been reported as a new therapeutic strategy for treating diabetes. However, the effect of SGLT2 inhibitors on the kidney is unknown. In addition, whether SGLT2 inhibitors have an anti-inflammatory or antioxidative stress effect is still unclear. In this study, to resolve these issues, we evaluated the effects of the SGLT2 inhibitor, dapagliflozin, using a mouse model of type 2 diabetes and cultured proximal tubular epithelial (mProx24) cells. Male db/db mice were administered 0.1 or 1.0 mg/kg of dapagliflozin for 12 weeks. Body weight, blood pressure, blood glucose, hemoglobin A1c, albuminuria and creatinine clearance were measured. Mesangial matrix accumulation and interstitial fibrosis in the kidney and pancreatic beta-cell mass were evaluated by histological analysis. Furthermore, gene expression of inflammatory mediators, such as osteopontin, monocyte chemoattractant protein-1 and transforming growth factor-beta, was evaluated by quantitative reverse transcriptase-PCR. In addition, oxidative stress was evaluated by dihydroethidium and NADPH oxidase 4 staining. Administration of 0.1 or 1.0 mg/kg of dapagliflozin ameliorated hyperglycemia, beta-cell damage and albuminuria in db/db mice. Serum creatinine, creatinine clearance and blood pressure were not affected by administration of dapagliflozin, but glomerular mesangial expansion and interstitial fibrosis were suppressed in a dose-dependent manner. Dapagliflozin treatment markedly decreased macrophage infiltration and the gene expression of inflammation and oxidative stress in the kidney of db/db mice. Moreover, dapagliflozin suppressed the high-glucose-induced gene expression of inflammatory cytokines and oxidative stress in cultured mProx24 cells. These data suggest that dapagliflozin ameliorates diabetic nephropathy by improving hyperglycemia along with inhibiting inflammation and oxidative stress.

    DOI: 10.1371/journal.pone.0100777

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  • N’-[4-(dipropylamino)benzylidene]-2-hydroxybenzohydrazide is a dynamin GTPase inhibitor that suppresses cancer cell migration and invasion by inhibiting actin polymerization . 査読

    Hiroshi Yamada, Tadashi Abe, Shun-Ai Li, Shota Tago, Peng Huang, Masami Watanabe, Satoru Ikeda, Naohisa Ogo, Akira Asai, Kohji Takei

    Biochem Biophys Res Commun   443 ( 2 )   511 - 517   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbrc.2013.11.118

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  • Metallothionein deficiency exacerbates diabetic nephropathy in streptozotocin-induced diabetic mice 査読

    Tachibana H, Ogawa D, Sogawa N, Asanuma M, Miyazaki I, Terami N, Hatanaka T, Horiguchi C. S, Nakatsuka A, Eguchi J, Wada J, Yamada H, Takei K, Makino H

    Am J Physiol Renal Physiol   306 ( 1 )   F105 - 15   2014年

  • Cancer stem cell-like characteristics of a CD133+ subpopulation in the J82 human bladder cancer cell line. 査読

    Peng Huang, Masami Watanabe, Haruki Kaku, Hideo Ueki, Hirofumi Noguchi, Morito Sugimoto, Takeshi Hirata, Hiroshi Yamada, Kohji Takei, Shaobo Zheng, Kai Xu, Yasutomo Nasu, Yasuyuki Fujii, Chunxiao Liu, Hiromi Kumon

    Molecular and Clinical Oncology   180 - 184   2013年1月

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    記述言語:英語  

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  • REIC/Dkk-3-encoding adenoviral vector as a potentially effective therapeutic agent for bladder cancer. 査読

    Hirata T, Watanabe M, Kaku H, Kobayashi Y, Yamada H, Sakaguchi M, Takei K, Huh NH, Nasu Y, Kumon H

    International journal of oncology   41   559 - 564   2012年8月

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    出版者・発行元:2  

    DOI: 10.3892/ijo.2012.1503

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  • A novel gene expression system for detecting viable bladder cancer cells 査読

    Hideo Ueki, Masami Watanabe, Haruki Kaku, Peng Huang, Shun-Ai Li, Kazuhiko Ochiai, Takeshi Hirata, Hirofumi Noguchi, Hiroshi Yamada, Kohji Takei, Yasutomo Nasu, Yuji Kashiwakura, Hiromi Kumon

    INTERNATIONAL JOURNAL OF ONCOLOGY   41 ( 1 )   135 - 140   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPANDIDOS PUBL LTD  

    A novel transcriptional system was developed that can robustly enhance cancer-specific gene expression. In the system, hTERT promoter-driven gene expression was enhanced by an advanced two-step transcriptional amplification (TSTA). This construct was used to develop a novel system for detection of bladder cancer cells. The current study evaluated the advanced TSTA system by examining the cancer-specific gene transcription in various bladder cancer cell lines. The system significantly enhanced cancer-specific luciferase gene expression in the bladder cancer cell lines in comparison to the previous expression system of one-step or conventional TSTA. The fold gain of the enhancement was significantly correlated to the telomerase activity of the cell lines. A green fluorescent protein (GFP) gene encoding plasmid vector was constructed where hTERT promoter-driving transcription is enhanced by the advanced TSTA to utilize the system for the imaging and detection of viable bladder cancer cells. The advanced TSTA-hTERT-GFP plasmid successfully induced cancer-specific gene expression, showing robust GFP expression in human bladder cancer cell lines, but no visible GFP expression in normal bladder urothelial cells. The control GFP plasm id with a CMV promoter yielded GFP expression in both normal bladder cells and cancer cells. The advanced TSTA-hTERT-GFP plasmid allowed selective visualization of viable human bladder cancer cells in mixed cell culture containing 10- and 100-fold more normal bladder urothelial cells. These findings indicate that the advanced TSTA-hTERT expressional system is a valuable tool for detecting viable bladder cancer cells. The current system can be applied for in vitro detection of bladder cancer cells in urine and other types of cancer cells disseminated in vivo.

    DOI: 10.3892/ijo.2012.1417

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  • Etiological Role of Dynamin in Charcot-Marie-Tooth Disease 査読

    Takei Kohji, Tanabe Kenji

    Peripheral Neuropathy – Advances in Diagnostic and Therapeutic Approaches   3   2012年

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  • The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice. 査読

    渡邊 利雄, Mai Suzuki, Hirokazu Tanaka, Akira Tanimura, Kenji Tanabe, Natsuko Oe, Shinya Rai, Syunsuke Kon, Manabu Fukumoto, Kohji Takei, Takaya Abe, Itaru Matsumura, Yuzuru Kanakura, Toshio Watanabe

    PLoS ONE   10.1371/journal.pone.0031854   2012年

  • Dynamin 2 in charcot-marie-tooth disease. 査読

    Tanabe Kenji, Takei Kohji

    Acta medica Okayama   66 ( 3 )   183   2012年

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  • Receptor Sorting within Endosomal Trafficking Pathway Is Facilitated by Dynamic Actin Filaments. 査読

    Ohashi Emiko, Tanabe Kenji, Henmi Yuji, Mesaki Kumi, Kobayashi Yuka, Takei Kohji

    PLoS ONE   6 ( 5 )   e19942   2011年

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    出版者・発行元:Public Library of Science  

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  • Disruption of microtubule network rescues aberrant actin comets in dynamin2-depleted cells. 査読

    Henmi Yuji, Tanabe Kenji, Takei Kohji

    PLoS ONE   6 ( 12 )   e28603   2011年

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    出版者・発行元:Public Library of Science  

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  • Dynamin 2 associates with microtubules at mitosis and regulates cell cycle progression. 査読

    Ishida Nobuhisa, Nakamura Yuichi, Tanabe Kenji, Li Shun-Ai, Takei Kohji

    Cell Structure and Function   36 ( 2 )   145   2011年

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  • Fission of tubular endosomes triggers endosomal acidification and movement. 査読

    Mesaki Kumi, Tanabe Kenji, Obayashi Masanori, Oe Natsuko, Takei Kohji

    PLoS ONE   6 ( 5 )   e19764   2011年

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    出版者・発行元:Public Library of Science  

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  • Receptor sorting and actin dynamics at early endosomes. 査読

    Tanabe Kenji, Ohashi Emiko, Henmi Yuji, Takei Kohji

    Communicative & Integrative Biology   4 ( 6 )   742   2011年

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    出版者・発行元:Landes Bioscience  

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  • Use of liposomes to study vesicular transport. 査読 国際誌

    Kohji Takei, Hiroshi Yamada, Tadashi Abe

    Methods in molecular biology (Clifton, N.J.)   606   531 - 42   2010年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.

    DOI: 10.1007/978-1-60761-447-0_36

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  • Dynasore, a dynamin inhibitor, suppresses lamellipodia formation and cancer cell invasion by destabilizing actin filaments. 査読

    Yamada H, Abe T, Li SA, Masuoka Y, Isoda M, Watanabe M, Nasu Y, Kumon H, Asai A, Takei K

    Biochem Biophys Res Commun   2009年12月

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    記述言語:英語  

    DOI: 10.1016/j.bbrc.2009.10.105

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  • Dynamic Interaction of Amphiphysin with N-WASP Regulates Actin Assembly 査読

    Hiroshi Yamada, Sergi Padilla-Parra, Sun-Joo Park, Toshiki Itoh, Mathilde Chaineau, Ilaria Monaldi, Ottavio Cremona, Fabio Benfenati, Pietro De Camilli, Maite Coppey-Moisan, Marc Tramier, Thierry Galli, Kohji Takei

    JOURNAL OF BIOLOGICAL CHEMISTRY   284 ( 49 )   34244 - 34256   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Amphiphysin 1, an endocytic adaptor concentrated at synapses that couples clathrin-mediated endocytosis to dynamin-dependent fission, was also shown to have a regulatory role in actin dynamics. Here, we report that amphiphysin 1 interacts with N-WASP and stimulates N-WASP- and Arp2/3-dependent actin polymerization. Both the Src homology 3 and the N-BAR domains are required for this stimulation. Acidicliposome-triggered, N-WASP dependent actin polymerization is strongly impaired in brain cytosol of amphiphysin 1 knock-out mice. FRET-FLIM analysis of Sertoli cells, where endogenously expressed amphiphysin 1 co-localizes with N-WASP in peripheral ruffles, confirmed the association between the two proteins in vivo. This association undergoes regulation and is enhanced by stimulating phosphatidylserine receptors on the cell surface with phosphatidylserine-containing liposomes that trigger ruffle formation. These results indicate that actin regulation is a key function of amphiphysin 1 and that such function cooperates with the endocytic adaptor role and membrane shaping/curvature sensing properties of the protein during the endocytic reaction.

    DOI: 10.1074/jbc.M109.064204

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  • Dynamin 2 is required for actin assembly in phagocytosis in Sertoli cells. 査読

    Otsuka A, Abe T, Watanabe M, Yagisawa H, Takei K, Yamada H

    Biochem Biophys Res Commun. 2009 Jan 16;378(3):478-82. Epub 2008 Nov 24.   478 - 482   2009年1月

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    記述言語:英語  

    DOI: 10.1016/j.bbrc.2008.11.066

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  • Major Cdk5-dependent phosphorylation sites of amphiphysin 1 are implicated in the regulation of the membrane binding and endocytosis. 査読

    Liang Shuang, Wei Fan-Yan, Wu Yu-Mei, Tanabe Kenji, Abe Tadashi, Oda Yoshiya, Yoshida Yumi, Yamada Hiroshi, Matsui Hideki, Tomizawa Kazuhito, Takei Kohji

    Journal of neurochemistry   102 ( 5 )   1466   2007年

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  • Norepinephrine triggers Ca2+-dependent exocytosis of 5-hydroxytryptamine from rat pinealocytes in culture. 査読

    Yamada H, Hayashi M, Uehara S, Kinoshita M, Muroyama A, Watanabe M, Takei K, Moriyama Y

    Journal of neurochemistry   81 ( 3 )   533 - 540   2002年5月

  • Identification and characterization of a synaptojanin 2 splice isoform predominantly expressed in nerve terminals. 査読

    Nemoto Y, Wenk MR, Watanabe M, Daniell L, Murakami T, Ringstad N, Yamada H, Takei K, De Camilli P

    J Biol Chem.   276 ( 44 )   41133 - 41142   2001年9月

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    記述言語:英語  

    DOI: 10.1074/jbc.M106404200

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▼全件表示

書籍等出版物

  • Use of liposomes to study vesicular transport

    Humana Press  2010年 

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  • Methods in Molecular Biology “Liposomes”

    Humana Press (USA), Springer Publishing Group,  2009年 

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  • 生体の科学55

    医学書院,東京  2004年 

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  • 文部科学省科学研究費特定領域研究C「ゲノム」2000年度報告書

    2001年 

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  • 平成12?13年度科学研究費補助金(基盤研究(B)(2))研究成果報告書

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MISC

  • 細胞操作と定量イメージングで知る細胞骨格ダイナミズム 細胞の形態形成における膜-細胞骨格の動的相互作用

    竹田 哲也, 藤瀬 賢志郎, 山田 浩司, 竹居 孝二

    日本細胞生物学会大会講演要旨集   72回   W3 - 5   2020年6月

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    記述言語:日本語   出版者・発行元:(一社)日本細胞生物学会  

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  • 腎糸球体ポドサイトにおけるアンフィファイジン1の機能

    山田 浩司, The Mon La, 竹田 哲也, 阿部 匡史, 淺沼 克彦, 竹居 孝二

    日本生化学会大会プログラム・講演要旨集   92回   [3P - 138]   2019年9月

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    記述言語:英語   出版者・発行元:(公社)日本生化学会  

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  • 腎糸球体ポドサイトにおけるダイナミンイソフォームの局在と機能

    阿部 匡史, The Mon La, 橘 洋美, 竹田 哲也, 竹居 孝二, 山田 浩司

    日本生化学会大会プログラム・講演要旨集   92回   [3P - 139]   2019年9月

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    記述言語:日本語   出版者・発行元:(公社)日本生化学会  

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  • 筋細胞膜リモデリングにおけるメカニカルストレス応答の解析

    藤瀬 賢志郎, 山田 浩司, 竹居 孝二, 竹田 哲也

    日本筋学会学術集会プログラム・抄録集   5回   107 - 107   2019年8月

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    記述言語:日本語   出版者・発行元:日本筋学会  

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  • ダイナミン複合体による新規の膜切断機構:クラスタラーゼ・モデル

    竹居孝二, 山田浩司, 竹田哲也

    生物物理(Web)   59 ( 5 )   2019年

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  • メカノエンザイム・ダイナミンGTPaseによるアクチン線維束化機構の解析

    山田 浩司, 阿部 匡史, 竹田 哲也, 高島 英造, 森田 将之, 竹居 孝二

    日本生物工学会大会講演要旨集   平成30年度   122 - 122   2018年8月

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    記述言語:日本語   出版者・発行元:(公社)日本生物工学会  

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  • 膜リモデリングおよびリン脂質代謝異常に起因する先天性ミオパチー発症機序の解明

    藤瀬 賢志郎, 背山 佳穂, 山下 恭加, 山田 浩司, 戸井 基道, 竹居 孝二, 竹田 哲也

    日本筋学会学術集会プログラム・抄録集   4回   110 - 110   2018年8月

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    記述言語:日本語   出版者・発行元:日本筋学会  

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  • ダイナミン2のシャルコー・マリー・トゥース病の原因変異は腎ポドサイトのアクチン再構成を阻害する

    和木田 夏輝, La The Mon, 隅田 健斗, 竹田 哲也, 阿部 匡史, 竹居 孝二, 山田 浩司

    生命科学系学会合同年次大会   2017年度   [2P - 1089]   2017年12月

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    記述言語:日本語   出版者・発行元:生命科学系学会合同年次大会運営事務局  

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  • ダイナミン-コルタクチンらせん状複合体の解析 機械的なアクチン線維束形成とアクチン脱重合保護作用

    阿部 匡史, 山田 浩司, 竹田 哲也, 内橋 貴之, 安藤 敏夫, 竹居 孝二

    生命科学系学会合同年次大会   2017年度   [2P - 0320]   2017年12月

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    記述言語:日本語   出版者・発行元:生命科学系学会合同年次大会運営事務局  

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  • エンドサイトーシス生物学の新展開 ダイナミンによる膜切断過程の動態イメージング

    竹田 哲也, 小財 稔矢, 楊 恵然, 石黒 大輝, 背山 佳穂, 熊谷 祐介, 阿部 匡史, 山田 浩司, 内橋 貴之, 安藤 敏夫, 竹居 孝二

    生命科学系学会合同年次大会   2017年度   [4AW17 - 2]   2017年12月

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    記述言語:英語   出版者・発行元:生命科学系学会合同年次大会運営事務局  

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  • GTP加水分解に共役したダイナミン依存的膜切断機構の高速原子間力顕微鏡解析

    竹田 哲也, 石黒 大輝, 楊 恵然, 小財 稔矢, 背山 佳穂, 熊谷 祐介, 山田 浩司, 内橋 貴之, 安藤 敏夫, 竹居 孝二

    日本細胞生物学会大会講演要旨集   69回   72 - 72   2017年5月

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    記述言語:日本語   出版者・発行元:(一社)日本細胞生物学会  

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  • Actin bundling by dynamin 2 and cortactin is implicated in cell migration by stabilizing filopodia in human non-small cell lung carcinoma cells 国際誌

    Hiroshi Yamada, Tetsuya Takeda, Hiroyuki Michiue, Tadashi Abe, Kohji Takei

    INTERNATIONAL JOURNAL OF ONCOLOGY   49 ( 3 )   877 - 886   2016年9月

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    記述言語:英語   出版者・発行元:SPANDIDOS PUBL LTD  

    The endocytic protein dynamin participates in the formation of actin-based membrane protrusions such as podosomes, pseudopodia, and invadopodia, which facilitate cancer cell migration, invasion, and metastasis. However, the role of dynamin in the formation of actin-based membrane protrusions at the leading edge of cancer cells is unclear. In this study, we demonstrate that the ubiquitously expressed dynamin 2 isoform facilitates cell migration by stabilizing F-actin bundles in filopodia of the lung cancer cell line H1299. Pharmacological inhibition of dynamin 2 decreased cell migration and filopodial formation. Furthermore, dynamin 2 and cortactin mostly colocalized along F-actin bundles in filopodia of serum-stimulated H1299 cells by immunofluorescent and immunoelectron microscopy. Knockdown of dynamin 2 or cortactin inhibited the formation of filopodia in serum-stimulated H1299 cells, concomitant with a loss of F-actin bundles. Expression of wild-type cortactin rescued the punctate-like localization of dynamin 2 and filopodial formation. The incubation of dynamin 2 and cortactin with F-actin induced the formation of long and thick actin bundles, with these proteins colocalizing at F-actin bundles. A depolymerization assay revealed that dynamin 2 and cortactin increased the stability of F-actin bundles. These results indicate that dynamin 2 and cortactin participate in cell migration by stabilizing F-actin bundles in filopodia. Taken together, these findings suggest that dynamin might be a possible molecular target for anticancer therapy.

    DOI: 10.3892/ijo.2016.3592

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  • Expression of a dynamin 2 mutant associated with Charcot-Marie-Tooth disease leads to aberrant actin dynamics and lamellipodia formation

    Hiroshi Yamada, Kinue Kobayashi, Yubai Zhang, Tetsuya Takeda, Kohji Takei

    NEUROSCIENCE LETTERS   628   179 - 185   2016年8月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    Specific mutations in dynamin 2 are linked to Charcot-Marie-Tooth disease (CMT), an inherited peripheral neuropathy. However, the effects of these mutations on dynamin function, particularly in relation to the regulation of the actin cytoskeleton remain unclear. Here, selected CMT-associated dynamin mutants were expressed to examine their role in the pathogenesis of CMT in U2OS cells. Ectopic expression of the dynamin CMT mutants 555 Delta 3 and K562E caused an approximately 50% decrease in serum stimulation dependent lamellipodia formation; however, only K562E caused aberrations in the actin cytoskeleton. Immunofluorescence analysis showed that the K562E mutation resulted in the disappearance of radially aligned actin bundles and the simultaneous appearance of F-actin clusters. Live-cell imaging analyses showed F-actin polymers of decreased length assembled into immobile clusters in K562E-expressing cells. The K562E dynamin mutant colocalized with the F-actin clusters, whereas its colocalization with clathrin-coated pit marker proteins was decreased. Essentially the same results were obtained using another cell line, HeLa and NG108-15 cells. The present study is the first to show the association of dynamin CMT mutations with aberrant actin dynamics and lamellipodia, which may contribute to defective endocytosis and myelination in Schwann cells in CMT. (C) 2016 The Authors. Published by Elsevier Ireland Ltd.

    DOI: 10.1016/j.neulet.2016.06.030

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  • 腎糸球体ポドサイトにおけるダイナミンアイソフォームの局在と機能

    橘 洋美, 竹田 哲也, 山田 浩司, 小川 大輔, 竹居 孝二

    日本細胞生物学会大会講演要旨集   68回   98 - 98   2016年5月

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    記述言語:日本語   出版者・発行元:(一社)日本細胞生物学会  

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  • Antiobesity Action of ACAM by Modulating the Dynamics of Cell Adhesion and Actin Polymerization in Adipocytes

    Kazutoshi Murakami, Jun Eguchi, Kazuyuki Hida, Atsuko Nakatsuka, Akihiro Katayama, Miwa Sakurai, Haruki Choshi, Masumi Furutani, Daisuke Ogawa, Kohji Takei, Fumio Otsuka, Jun Wada

    DIABETES   65 ( 5 )   1255 - 1267   2016年5月

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    記述言語:英語   出版者・発行元:AMER DIABETES ASSOC  

    Coxsackie virus and adenovirus receptor-like membrane protein (CLMP) was identified as the tight junction-associated transmembrane protein of epithelial cells with homophilic binding activities. CLMP is also recognized as adipocyte adhesion molecule (ACAM), and it is upregulated in mature adipocytes in rodents and humans with obesity. Here, we present that aP2 promoter-driven ACAM transgenic mice are protected from obesity and diabetes with the prominent reduction of adipose tissue mass and smaller size of adipocytes. ACAM is abundantly expressed on plasma membrane of mature adipocytes and associated with formation of phalloidin-positive polymerized form of cortical actin (F-actin). By electron microscopy, the structure of zonula adherens with an intercellular space of approximate to 10-20 nm was observed with strict parallelism of the adjoining cell membranes over distances of 1-20 m, where ACAM and -actin are abundantly expressed. The formation of zonula adherens may increase the mechanical strength, inhibit the adipocyte hypertrophy, and improve the insulin sensitivity.

    DOI: 10.2337/db15-1304

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  • PtdIns4 KIIα generates endosomal PtdIns(4)P and is required for receptor sorting at early endosomes

    Yuji Henmi, Yoshiaki Morikawa, Natsuko Oe, Narumi Ikeda, Akikazu Fujita, Kohji Takei, Shane Minogue, Kenji Tanabe

    Molecular Biology of the Cell, Cell regulation   27 ( 6 )   990 - 1001   2016年3月

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  • Possible role of cortactin phosphorylation by protein kinase Cα in actin-bundle formation at growth cone

    Yamada, H, Kikuchi, T, Masumoto, T, Wei, F.Y, Abe, T, Takeda, T, Nishiki, T, Tomizawa, K, Watanabe, M, Matsui, H, Takei, K

    Biol Cell   107 ( 9 )   319 - 330   2015年9月

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  • 腎糸球体ポドサイト分化におけるダイナミンGTPアーゼの役割

    橘洋美, 竹田哲也, 山田浩司, 小川大輔, 竹居孝二

    日本生化学会大会(Web)   88th   2015年

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  • Stabilization of actin bundles by a dynamin 1/cortactin ring complex is necessary for growth cone filopodia.

    Yamada, H, Abe, T, Satoh, A, Okazaki, N, Tago, S, Kobayashi, K, Yoshida, Y, Oda, Y, Watanabe, M, Tomizawa, K, Matsui, H, Takei, K

    Journal of Neuroscience   33 ( 10 )   4514 - 4526   2015年

  • 肺がん細胞株における細胞運動を司るダイナミン2によるアクチン動態制御

    阿部匡史, 山田浩司, 竹田哲也, 竹居孝二

    日本生化学会大会(Web)   87th   2014年

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  • Dynaminを標的にした抗がん剤の開発

    岡崎奈奈, 阿部匡史, 有田美香子, 多湖翔太, 永井さや, 池田敏, 小郷尚久, 浅井章良, 山田浩司, 竹居孝二

    日本生化学会大会(Web)   85th   2012年

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  • アンフィファイジンとN-WASPのダイナミックな相互作用は,アクチン重合を制御する

    山田浩司, PADILLA-PARRA Sergi, 朴宣奏, 朴宣奏, 伊藤俊樹, CHAINEAU Mathilde, CHAINEAU Mathilde, MONALDI Ilaria, CREMONA Ottavio, BENFENATI Fabio, CAMILLI Pietro De, COPPEY-MOISAN Maiete, TRAMIER Marc, GALLI Thierry, GALLI Thierry, 竹居孝二

    岡山医学会雑誌   123 ( 1 )   2011年

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  • ダイナミン/コルタクチン複合体によるアクチン細胞骨格制御機構

    竹居孝二, 山田浩司, 阿部匡

    生体膜と薬物の相互作用シンポジウム講演要旨集   33rd   2011年

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  • Dynamin 1/cortactin complex mechanically bundles actin filaments and supports the formation of growth cone filopodia

    Kohji Takei, Tadashi Abe, Yoshihiro Kawada, Hiroshi Yamada

    NEUROSCIENCE RESEARCH   68   E88 - E88   2010年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER IRELAND LTD  

    DOI: 10.1016/j.neures.2010.07.156

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  • Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development.

    Zhang K, Watanabe M, Kashiwakura Y, Li SA, Edamura K, Huang P, Yamaguchi K, Nasu Y, Kobayashi Y, Sakaguchi M, Ochiai K, Yamada H, Takei K, Ueki H, Huh NH, Li M, Kaku H, Na Y, Kumon H

    Int J Oncol   37 ( 6 )   1495 - 1501   2010年

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  • ダイナミン2による微小管の動態制御とCharcot-Marie-Tooth病

    岡山医学会雑誌   122 ( 2 )   113 - 117   2010年

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  • Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development

    Int. J. Oncol.   122   113 - 117   2010年

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  • アンフィファイジンとN-WASPのダイナミックな相互作用がアクチン重合を制御する(Dynamic interation of amphiphysin with N-WASP regulates actin assembly)

    山田浩司, セルジ=パディラパーラ, スンジュー=パク, 伊藤俊樹, 名省略, 、ファビオ=ベンフェナティ, ピエトロ=デカメリ, マイテ=コペイモイソン, マルコ=トラミア, テリー=ガリ, 竹居孝二

    ジャーナル・オブ・バイロロジカル・ケミストリー(J. Biol. Chem.)   68   E136 - E136   2010年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)  

    DOI: 10.1016/j.neures.2010.07.2175

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  • Dynamic instability of microtubules requires dynamin 2 and is impaired in a Charcot-Marie-Tooth mutant

    Kenji Tanabe, Kohji Takei

    JOURNAL OF CELL BIOLOGY   185 ( 6 )   939 - 948   2009年6月

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    記述言語:英語   出版者・発行元:ROCKEFELLER UNIV PRESS  

    Dynamin is a fission protein that participates in endocytic vesicle formation. Although dynamin was originally identified as a microtubule-binding protein, the physiological relevance of this function was unclear. Recently, mutations in the ubiquitously expressed dynamin 2 (dyn2) protein were found in patients with Charcot-Marie-Tooth (CMT) disease, which is an inherited peripheral neuropathy. In this study, we show that one of these mutations, 551 Delta 3, induces prominent decoration of microtubules with the mutant dyn2. Dyn2 was required for proper dynamic instability of microtubules, and this was impaired in cells expressing the 551 Delta 3 mutant, which showed a remarkable increase in microtubule acetylation, a marker of stable microtubules. Depletion of endogenous dyn2 with a small interfering RNA also resulted in the accumulation of stable microtubules. Furthermore, the formation of mature Golgi complexes, which depends on microtubule-dependent membrane transport, was impaired in both dyn2 knockdown cells and cells expressing the 551 Delta 3 mutant. Collectively, our results suggest that dyn2 regulates dynamic instability of microtubules, which is essential for organelle motility, and that this function may be impaired in CMT disease.

    DOI: 10.1083/jcb.200803153

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  • CaMキナーゼIα誘発性のDrp1リン酸化はミトコンドリア形態を制御する(CaM kinase Iα-induced phosphorylation of Drp1 regulates mitochondrial morphology)

    韓 小建, 陸 雲飛, 李 順愛, 貝塚 多久, 佐藤 泰史, 富澤 一仁, アンガス・シ・ネアン, 竹居 孝二, 松井 秀樹, 松下 正之

    日本生理学雑誌   71 ( 2 )   68 - 68   2009年2月

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    記述言語:英語   出版者・発行元:(一社)日本生理学会  

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  • REGULATION OF MITOCHONDRIAL DYNAMICS AND MORPHOLOGY BY CHANNEL SPECIFIC CALCIUM SIGNALING

    Xiao-Jian Han, Yun-Fei Lu, Shun-Ai Li, Kazuhito Tomizawa, Kohji Takei, Masayuki Matsushita, Hideki Matsui

    JOURNAL OF PHYSIOLOGICAL SCIENCES   59   245 - 245   2009年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:SPRINGER TOKYO  

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  • Dynamin 2 Cooperates with Amphiphysin 1 in Phagocytosis in Sertoli Cells

    Akira Nakanishi, Tadashi Abe, Masami Watanabe, Kohji Takei, Hiroshi Yamada

    ACTA MEDICA OKAYAMA   62 ( 6 )   385 - 391   2008年12月

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    記述言語:英語   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    Testicular Sertoli cells highly express dynamin 2 and amphiphysin 1. Here we demonstrate that dynamin 2 is implicated in phosphatidylserine (PS)-dependent phagocytosis in Sertoli cells. Immunofluorescence and dual-live imaging revealed that dynamin 2 and amphiphysin 1 accumulate simultaneously at ruffles. These proteins are specifically bound in vitro. Over-expression of dominant negative dynamin 2 (K44A) inhibits liposome-uptake and leads to the mis-localization of amphiphysin 1. Thus, the cooperative function of dynamin 2 and amphiphysin 1 in PS-dependent phagocytosis is strongly suggested.

    DOI: 10.18926/AMO/30954

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  • CaM kinase Iα-induced phosphorylation of Drp1 regulates mitochondrial morphology

    Han XJ, Lu YF, Li SA, Kaitsuka T, Sato Y, Tomizawa K, Nairn AC, Takei K, Matsui H, Matsushita M

    J Cell Biol   182 ( 3 )   573 - 585   2008年8月

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  • Regulation of clathrin-mediated endocytosis by p53

    Yoshie Endo, Atsumi Sugiyama, Shun-Ai Li, Kazuji Ohmori, Hirokazu Ohata, Yusuke Yoshida, Masabumi Shibuya, Kohji Takei, Masato Enari, Yoichi Taya

    GENES TO CELLS   13 ( 4 )   375 - 385   2008年4月

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    記述言語:英語   出版者・発行元:BLACKWELL PUBLISHING  

    The p53 gene encodes a multi-functional protein to prevent tumorigenesis. Although there have been many reports of the nuclear functions of p53, little is known about the cytosolic functions of p53. Here, we found that p53 is present in cytosol as well as nuclei under unstressed conditions and binds to clathrin heavy chain (CHC). CHC is known to play a role in receptor-mediated endocytosis. Based on our findings, we examined the effect of p53 on clathrin-mediated endocytosis of epidermal growth factor receptor (EGFR). Surprisingly, p53 co-localized with CHC at the plasma membrane in response to EGF stimulation. In cells with ablated p53 expression by RNAi, EGFR internalization was delayed and intracellular signaling from EGFR was altered. Thus, our findings provide evidence that cytosolic p53 may participate in the regulation of clathrin-mediated endocytosis to control the correct signaling from EGFR.

    DOI: 10.1111/j.1365-2443.2008.01172.x

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  • SNX9 regulates tubular invagination of the plasma membrane through interaction with actin cytoskeleton and dynamin 2

    Narae Shin, Namhui Ahn, Belle Chang-Ileto, Joohyun Park, Kohji Takei, Sang-Gun Ahn, Soo-A Kim, Gilbert Di Paolo, Sunghoe Chang

    JOURNAL OF CELL SCIENCE   121 ( 8 )   1252 - 1263   2008年4月

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    記述言語:英語   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    Dynamic membrane remodeling during intracellular trafficking is controlled by the intricate interplay between lipids and proteins. BAR domains are modules that participate in endocytic processes by binding and deforming the lipid bilayer. Sorting nexin 9 (SNX9), which functions in clathrin-mediated endocytosis, contains a BAR domain, however, the properties of this domain are not well understood. Here we show that SNX9 shares many properties with other BAR domain-containing proteins, such as amphiphysin and endophilin. SNX9 is able to deform the plasma membrane, as well as liposomes, into narrow tubules and recruit N-WASP and dynamin 2 to these tubules via its SH3 domain. SNX9-induced tubulation is antagonized by N-WASP and dynamin 2 while it is enhanced by perturbation of actin dynamics. However, SNX9 also has several unique properties. The tubulating activity requires the BAR and PX domains, as well as the low-complexity (LC) domain, which binds the Arp2/3 complex. SNX9 also binds to PtdIns(4)P-5-kinases via its PX domain and its tubulating activity is regulated by phosphoinositides. In addition, the kinase activity of PtdIns(4)P-5-kinases is stimulated by interaction with SNX9, suggesting a positive feedback interaction between SNX9 and PtdIns(4)P-5-kinases. These results suggest that SNX9 functions in the coordination of membrane remodeling and fission via interactions with actin-regulating proteins, endocytic proteins and PtdIns(4,5)P-2-metabolizing enzymes.

    DOI: 10.1242/jcs.016709

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  • Involvement of calcineurin in glutamate-induced mitochondrial dynamics in neurons

    Xiao-Jian Han, Yun-Fei Lu, Shun-Ai Li, Kazuhito Tomizawa, Kohji Takei, Masayuki Matsushita, Hideki Matsui

    NEUROSCIENCE RESEARCH   60 ( 1 )   114 - 119   2008年1月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    Alterations in the morphology and movement of mitochondria influence neuronal viability. However, the precise mechanisms of such alterations are unclear. In this study, we showed calcineurin was involved in the regulation of mitochondrial dynamics. Glutamate stimulation inhibited mitochondrial movement and decreased mitochondrial length in neurons. FK506 and cyclosporine A, calcineurin inhibitors, attenuated the effects of glutamate on mitochondrial dynamics. It was also found that glutamate treatment dephosphorylated, a proapoptotic protein, Bad and promoted its translocation to mitochondria in neurons via calcineurin. These results provide important new insights into intracellular signaling pathways that regulate mitochondrial dynamics and neuronal cell death. (c) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

    DOI: 10.1016/j.neures.2007.09.012

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  • Amphiphysin 1 is important for actin polymerization During phagocytosis

    Hiroshi Yamada, Emiko Ohashi, Tadashi Abe, Norihiro Kusumi, Shun-AI Li, Yumi Yoshida, Masami Watanabe, Kazuhito Tomizawa, Yuji Kashiwakura, Hiromi Kumon, Hideki Matsui, Kohji Takei

    MOLECULAR BIOLOGY OF THE CELL   18 ( 11 )   4669 - 4680   2007年11月

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    記述言語:英語   出版者・発行元:AMER SOC CELL BIOLOGY  

    Amphiphysin 1 is involved in clathrin-mediated endocytosis. In this study, we demonstrate that amphiphysin 1 is essential for cellular phagocytosis and that it is critical for actin polymerization. Phagocytosis in Sertoli cells was induced by stimulating phosphatidylserine receptors. This stimulation led to the formation of actin-rich structures, including ruffles, phagocytic cups, and phagosomes, all of which showed an accumulation of amphiphysin 1. Knocking out amphiphysin 1 by RNA interference in the cells resulted in the reduction of ruffle formation, actin polymerization, and phagocytosis. Phagocytosis was also drastically decreased in amph 1(-/-) Sertoli cells. In addition, phosphatidylinositol-4,5-bisphosphate-induced actin polymerization was decreased in the knockout testis cytosol. The addition of recombinant amphiphysin 1 to the cytosol restored the polymerization process. Ruffle formation in small interfering RNA-treated cells was recovered by the expression of constitutively active Rac1, suggesting that amphiphysin 1 functions upstream of the protein. These findings support that amphiphysin 1 is important in the regulation of actin dynamics and that it is required for phagocytosis.

    DOI: 10.1091/mbc.E07-04-0296

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  • cAMP-response element binding protein (CREB) regulates cyclosporine-A-mediated down-regulation of cathepsin B and L synthesis

    Kazuhiro Omori, Koji Naruishi, Tomoko Yamaguchi, Shun-Ai Li, Mayumi Yamaguchi-Morimoto, Kaori Matsuura, Hideo Arai, Kohji Takei, Shogo Takashiba

    CELL AND TISSUE RESEARCH   330 ( 1 )   75 - 82   2007年10月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Cyclosporin A (CsA) is an immunosuppressant with severe side effects including gingival overgrowth. We have previously reported that CsA impairs the activity of the lysosomal enzymes cathepsin B and L in human gingival fibroblasts (HGFs). Here, we have examined the effects of CsA on the DNA-binding activity of the cyclic AMP response element-binding protein (CREB) and cell viability, and the effects of CREB on cathepsin B and L synthesis and activity in HGFs. We have confirmed that CsA down-regulates cathepsin B and L synthesis. Further, CsA has no effect on cell viability and dramatically impairs CREB-DNA binding activity. Importantly, the synthesis of cathepsin B and L is down-regulated, and their activity is also significantly impaired in HGFs transfected with plasmid expressing dominant-negative CREB. These results suggest that CREB is essential for the CsA-mediated down-regulation of cathepsin B and L synthesis in HGFs.

    DOI: 10.1007/s00441-007-0457-8

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  • Truncations of amphiphysin I by calpain inhibit vesicle endocytosis during neural hyperexcitation

    Yumei Wu, Shuang Liang, Yoshiya Oda, Iori Ohmori, Tei-Ichi Nishiki, Kohji Takei, Hideki Matsui, Kazuhito Tomizawa

    EMBO JOURNAL   26 ( 12 )   2981 - 2990   2007年6月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Under normal physiological conditions, synaptic vesicle endocytosis is regulated by phosphorylation and Ca2+-dependent dephosphorylation of endocytic proteins such as amphiphysin and dynamin. To investigate the regulatory mechanisms that may occur under the conditions of excessive presynaptic Ca2+ influx observed preceding neural hyperexcitation, we examined hippocampal slices following high-potassium or high-frequency electrical stimulation (HFS). In both cases, three truncated forms of amphiphysin I resulted from cleavage by the protease calpain. In vitro, the binding of truncated amphiphysin I to dynamin I and copolymerization into rings with dynamin I were inhibited, but its interaction with liposomes was not affected. Moreover, overexpression of the truncated form of amphiphysin I inhibited endocytosis of transferrin and synaptic vesicles. Inhibiting calpain prevented HFS-induced depression of presynaptic transmission. Finally, calpain-dependent amphiphysin I cleavage attenuated kainate-induced seizures. These results suggest that calpain-dependent cleavage of amphiphysin I inhibits synaptic vesicle endocytosis during neural hyperexcitation and demonstrate a novel post-translational regulation of endocytosis.

    DOI: 10.1038/sj.emboj.7601741

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  • Implication of amphiphysin 1 and dynamin 2 in tubulobulbar complex formation and spermatid release

    Norihiro Kusumi, Masami Watanabe, Hiroshi Yamada, Shun-Ai Li, Yuji Kashiwakura, Takashi Matsukawa, Atsushi Nagai, Yasutomo Nasu, Hiromi Kumon, Kohji Takei

    CELL STRUCTURE AND FUNCTION   32 ( 2 )   101 - 113   2007年

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    記述言語:英語   出版者・発行元:JAPAN SOC CELL BIOLOGY  

    Tubulobulbar complexes (TBCs) are composed of several tubular invaginations formed at the plasma membrane of testicular Sertoli cells. TBCs are transiently formed at the contact region with spermatids at spermatogenic stage VII in rat and mouse, and such TBC formation is prerequisite for spermatid release. Since the characteristic structure of TBCs suggests that the molecules implicated in endocytosis could be involved in TBC formation, we here investigated the localization and physiological roles of endocytic proteins, amphiphysin 1 and dynamin 2, at TBCs. We demonstrated by immunofluorescence that the endocytic proteins were concentrated at TBCs, where they colocalized with cytoskeletal proteins, such as actin and vinculin. Immunoelectron microscopy disclosed that both amphiphysin 1 and dynamin 2 were localized on TBC membrane. Next, we histologically examined the testis from amphiphysin 1 deficient {Amph(-/-)} mice. Morphometric analysis revealed that the number of TBCs was significantly reduced in Amph(-/-). The ratio of stage VIII seminiferous tubules was increased, and the ratio of stage IX was conversely decreased in Amph(-/-). Moreover, unreleased spermatids in stage VIII seminiferous tubules were increased in Amph(-/-), indicating that spermatid release and the following transition from stage VIII to IX was prolonged in Amph(-/-) mice. These results suggest that amphiphysin 1 and dynamin 2 are involved in TBC formation and spermatid release at Sertoli cells.

    DOI: 10.1247/csf.07024

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  • A human beta-cell line for transplantation therapy to control type 1 diabetes

    M Narushima, N Kobayashi, T Okitsu, Y Tanaka, SA Li, Y Chen, A Miki, K Tanaka, S Nakaji, K Takei, AS Gutierrez, JD Rivas-Carrillo, N Navarro-Alvarez, HS Jun, KA Westerman, H Noguchi, JRT Lakey, P Leboulch, N Tanaka, JW Yoon

    NATURE BIOTECHNOLOGY   23 ( 10 )   1274 - 1282   2005年10月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    A human pancreatic b-cell line that is functionally equivalent to primary b-cells has not been available. We established a reversibly immortalized human b-cell clone ( NAKT-15) by transfection of primary human b-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telomerase reverse transcriptase ( hTERT) cDNAs flanked by paired loxP recombination targets, which allow deletion of SV40T and TERT by Cre recombinase. Reverted NAKT-15 cells expressed b-cell transcription factors ( Isl-1, Pax 6, Nkx 6.1, Pdx-1), prohormone convertases 1/3 and 2, and secretory granule proteins, and secreted insulin in response to glucose, similar to normal human islets. Transplantation of NAKT-15 cells into streptozotocin-induced diabetic severe combined immunodeficiency mice resulted in perfect control of blood glucose within 2 weeks; mice remained normoglycemic for longer than 30 weeks. The establishment of this cell line is one step toward a potential cure of diabetes by transplantation.

    DOI: 10.1038/nbt1145

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  • Analysis of the major cdk5 phosphorylation sites of amphiphysin 1

    Shuang Liang, Yumi Yoshida, Kazuhito Tomizawa, Tadashi Abe, Hiroshi Yamada, Hideki Matsui, Kohji Takei

    CELL STRUCTURE AND FUNCTION   30   70 - 70   2005年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:JAPAN SOC CELL BIOLOGY  

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  • Localization of dynamin2 during mitosis

    Nobuhisa Ishida, Shun-Ai Li, Yuuichi Nakamura, Hiroshi Yamada, Toshio Sugahara, Kohji Takei

    CELL STRUCTURE AND FUNCTION   30   58 - 58   2005年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:JAPAN SOC CELL BIOLOGY  

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  • Dynamin 2 is involved in PS-dependent phagocytosis in rat Sertoli cells

    O. Hiroshi Yamada, ShunAI Li, Norihiro Kusumi, Masami Watanabe, Yuji Kashiwakura, Kazuhito Tomizawa, Hiromi Kumon, Kohji Takei

    CELL STRUCTURE AND FUNCTION   30   73 - 73   2005年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:JAPAN SOC CELL BIOLOGY  

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  • Localization of amphiphysin1 and dynamin2 in tubulobulbar complexes (TBC) formation and sperm release at Sertoli cells

    Norihiro Kusumi, Masami Watanabe, Hiroshi Yamada, Shun-Ai Li, Yuji Kashiwakura, Atsushi Nagai, Yasutomo Nasu, Hiromi Kumon, Pietro De Camilli, Kohji Takei

    CELL STRUCTURE AND FUNCTION   30   71 - 71   2005年6月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:JAPAN SOC CELL BIOLOGY  

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  • Regulatory mechanisms of dynamin-dependent endocytosis

    K Takei, Y Yoshida, H Yamada

    JOURNAL OF BIOCHEMISTRY   137 ( 3 )   243 - 247   2005年3月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:JAPANESE BIOCHEMICAL SOC  

    Extensive studies on endocytosis in the last decade have resulted in identification of several key molecules that function in clathrin- and dynamin-dependent endocytosis. Most endocytic molecules contain multiple binding motifs that mediate protein-protein or protein-lipid interactions, which must be modulated spatially and temporally during endocytosis. Regulation of these interactions is the molecular basis of regulatory mechanisms involved in endocytosis. This review first describes current models of the mechanism of dynamin-dependent fission, then introduces several mechanisms that modulate dynamin GTPase activity and dynamin-dependent vesicle formation. Such mechanisms include regulation by inositol phospholipids, especially phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P-2], and their metabolism. It concludes by describing the regulation of dynamin 1 by its binding partner, amphiphysin 1, and regulation by cyclin-dependent kinase 5 (Cdk5)-dependent phosphorylation of dynamin 1 and amphiphysin 1. These mechanisms help endocytic molecules to function properly, and cooperatively regulate dynamin-dependent endocytosis.

    DOI: 10.1093/jb/mvi052

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  • Immunohistochemical localization of Klotho protein in brain, kidney, and reproductive organs of mice

    SA Li, M Watanabe, H Yamada, A Nagai, M Kinuta, K Takei

    CELL STRUCTURE AND FUNCTION   29 ( 4 )   91 - 99   2004年12月

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    記述言語:英語   出版者・発行元:JAPAN SOC CELL BIOLOGY  

    Klotho mutant mouse (kl-/-), a mouse model for human aging. exhibits various phenotypes in a wide range of organs including arteriosclerosis, neural degeneration. skin and gonadal atrophy, pulmonary emphysema, calcification of soft tissues, and cognition impairment. Klotho mRNA. however. is expressed only in brain, kidney, reproductive organs, pituitary gland, and parathyroid gland. Therefore it remains to be elucidated how lack of Klotho protein in these limited organs leads to the variery of phenotypes. To shed light on mechanisms by which Klotho protein acts on distant targets, we examined localization of Klotho protein in brain. kidney. and reproductive organs, and analyzed brain and kidney in kl-/- mice searching for changes in target regions in these organs. In brain, Klotho proteins were localized at choroid plexus, where the proteins were dominantly localized at the apical plasma membrane of ependymal cells. In kl-/- brain, reduction of synapses was evident in hippocampus, suggesting a role of Klotho as a humoral factor in cerebrospinal fluid. Klotho proteins in kidney localized at distal renal tubules. Interestingly, in kl-/- mice, type IIa Na/phosphate (Pi) cotransporters, which function at the proximal renal tubules in reabsorption of phosphate ions. were translocated. This suggests that Klotho protein in kidney is implicated in calcium homeostasis which regulates localization of type IIa Na/Pi cotransporters via parathyroid hormone (PTH). Klotho proteins in reproductive organs were expressed only in mature germ cells, although in kl-/- mice germ cell maturation was arrested at earlier stages. Thus, Klotho proteins not only function as a humoral factor, but also are implicated in hormonal regulation. which may explain why mutation of klotho gene results in a variety of phenotypes.

    DOI: 10.1247/csf.29.91

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  • Dynamin-2 regulates oxidized low-density lipoprotein-induced apoptosis of vascular smooth muscle cell

    Y Kashiwakura, M Watanabe, N Kusumi, K Sumiyoshi, Y Nasu, H Yamada, T Sawamura, H Kumon, K Takei, H Daida

    CIRCULATION   110 ( 21 )   3329 - 3334   2004年11月

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    記述言語:英語   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Background-On exposure to oxidized low-density lipoprotein (oxLDL), vascular cells generally undergo apoptosis, which is one of the major pathogenic factors of atherosclerosis. In this study, we examined the role of dynamin ( a crucial GTPase protein in endocytosis) in oxLDL-induced apoptosis of vascular smooth muscle cells (VSMC).
    Methods and Results-After oxLDL stimulation, dynamin-2 colocalized with LOX-1 around the cell surface, as well as oxLDL in the cytoplasm, suggesting that dynamin-2 was involved in scavenger receptor-mediated oxLDL endocytosis. Downregulation of dynamin-2 induced by dynamin-2 dominant negative plasmid (K44A) resulted in a decrease of oxLDL uptake and thereby in a reduction of apoptosis. These data demonstrated that dynamin-2 was involved in oxLDL-induced apoptosis via the oxLDL endocytotic pathway. On the other hand, dynamin-2 wild-type plasmid transfection promoted oxLDL-induced apoptosis without increasing oxLDL uptake. Interestingly, the p53 inhibitor pifithrin-alpha (PFT) significantly reduced apoptosis promoted by wild-type dynamin-2 (78% reduction compared with the PFT[-] condition). These results indicated that dynamin-2 enhanced oxLDL-induced apoptosis of VSMC by participating in the p53 pathway, probably as a signal transducer. Moreover, we demonstrated that, in advanced plaques of apolipoprotein E-/- mice, dynamin-2 expression was often enhanced in apoptotic VSMC, suggesting that dynamin-2 might participate in apoptosis of VSMC even in vivo.
    Conclusions - Our data demonstrated that dynamin-2 at least partially regulated oxLDL-induced apoptosis of VSMC by participating in 2 independent pathways: the oxLDL endocytotic pathway and the p53 pathway. These findings suggest that dynamin-2 may serve as a new research or therapeutic target in vascular disease.

    DOI: 10.1161/01.CIR.0000147828.86593.85

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  • The stimulatory action of amphiphysin on dynamin function is dependent on lipid bilayer curvature

    Y Yoshida, M Kinuta, T Abe, S Liang, K Araki, O Cremona, G Di Paolo, Y Moriyama, T Yasuda, P De Camilli, K Takei

    EMBO JOURNAL   23 ( 17 )   3483 - 3491   2004年9月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Amphiphysin is a major dynamin-binding partner at the synapse; however, its function in fission is unclear. Incubation of large unilamellar liposomes with mice brain cytosol led to massive formation of small vesicles, whereas cytosol of amphiphysin 1 knockout mice was much less efficient in this reaction. Vesicle formation from large liposomes by purified dynamin was also strongly enhanced by amphiphysin. In the presence of liposomes, amphiphysin strongly affected dynamin GTPase activity and the recruitment of dynamin to the liposomes, but this activity was highly dependent on liposome size. Deletion from amphiphysin of its central proline-rich stretch dramatically potentiated its effect on dynamin, possibly by relieving an inhibitory intramolecular interaction. These results suggest a model in which maturation of endocytic pits correlates with the oligomerization of dynamin with either amphiphysin or other proteins with similar domain structure. Formation of these complexes is coupled to the activation of dynamin GTPase activity, thus explaining how deep invagination of the pit leads to fission.

    DOI: 10.1038/sj.emboj.7600355

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  • Dynamin及びAmphiphysinのリン酸化-脱リン酸化による小胞形成能変化

    絹田 正裕, 荒木 健太, 阿部 匡史, 梁 爽, 吉田 祐美, 山田 浩司, 永松 知洋, 富澤 一仁, 松井 秀樹, 保田 立二, 竹居 孝二

    生化学   76 ( 3 )   307 - 307   2004年3月

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    記述言語:日本語   出版者・発行元:(公社)日本生化学会  

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  • ダイナミンとエンドサイトーシス。

    竹居孝二

    蛋白質核酸酵素 増刊『細胞における蛋白質の一生』   49,938-941   2004年

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  • Cophosphorylation of amphiphysin 1 and dynamin 1 by Cdk5 regulates clathrin-mediated endocytosis of synaptic vesicles

    K Tomizawa, S Sunada, YF Lu, Y Oda, M Kinuta, T Ohshima, T Saito, FY Wei, M Matsushita, ST Li, K Tsutsui, S Hisanaga, K Mikoshiba, K Takei, H Matsui

    JOURNAL OF CELL BIOLOGY   163 ( 4 )   813 - 824   2003年11月

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    記述言語:英語   出版者・発行元:ROCKEFELLER UNIV PRESS  

    It has been thought that clathrin-mediated endocytosis is regulated by phosphorylation and dephosphorylation of many endocytic proteins, including amphiphysin I and dynamin I. Here, we show that Cdk5/p35-dependent cophosphorylation of amphiphysin I and dynamin I plays a critical role in such processes. Cdk5 inhibitors enhanced the electric stimulation-induced endocytosis in hippocampal neurons, and the endocytosis was also enhanced in the neurons of p35-deficient mice. Cdk5 phosphorylated the proline-rich domain of both amphiphysin I and dynamin I in vitro and in vivo. Cdk5-dependent phosphorylation of amphiphysin I inhibited the association with P-adaptin. Furthermore, the phosphorylation of dynamin I blocked its binding to amphiphysin I. The phosphorylation of each protein reduced the copolymerization into a ring formation in a cell-free system. Moreover, the phosphorylation of both proteins completely disrupted the copolymerization into a ring formation. Finally, phosphorylation of both proteins was undetectable in p35-deficient mice.

    DOI: 10.1083/jcb.200308110

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  • Formation of meso, N-diphenylprotoporphyrin IX by an aerobic reaction of phenylhydrazine with oxyhemoglobins

    A Nakanishi, K Kinuta, T Abe, K Araki, Y Yoshida, S Liang, SA Li, K Takei, M Kinuta

    ACTA MEDICA OKAYAMA   57 ( 5 )   249 - 256   2003年10月

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    記述言語:英語   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    Administration of phenylhydrazine to rabbits resulted in the denaturation of hemoglobins in erythrocytes, causing the formation of intracellular precipitates known as Heinz bodies, severe hemolytic anemia, and reticulocytosis. To elucidate the molecular mechanism of the destabilization, we allowed human oxyhemoglobins to react aerobically with phenylhydrazine. After treatment with acetic acid/HCl and H2SO4/methanol, the chloroform extract contained blue-green pigments of major products accompanied by different minor products. Each product was isolated by column chromatography. By fast-atom-bombardment mass spectrometry (FAB-MS) and proton nuclear magnetic resonance (H-1-NMR) spectrometry, dimethyl esters of N-phenylprotoporphyrin IX and meso, N-diphenylprotoporphyrin IX were determined. Other major products also were determined to be dimethyl esters of triphenyl- and tetraphenyl-substituted protoporphyrins by FAB-MS. The formation of meso, N-diphenylprotoporphyrin indicated that the addition of a phenyl radical to the meso-carbon atom of the protoporphyrin ring occurred. Triphenyl and tetraphenyl adducts also indicated the formation of phenyl radicals in the aerobic reaction of phenylhydrazine with oxyhemoglobins. From these results, we suggest that the formation of phenyl radicals and the replacement of heme with phenyl-substituted protoporphyrins cause the destabilization of hemoglobins to induce Heinz bodies and hemolytic anemia with phenylhydrazine.

    DOI: 10.18926/AMO/32823

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  • Formation of S-[2-carboxy-1-(1H-imidazol-4-yl) ethyl]glutathione, a new metabolite of L-histidine, from cis-urocanic acid and glutathione by the action of glutathione S-transferase

    M Kinuta, K Kinuta, H Yamada, T Abe, Y Yoshida, K Araki, SA Li, A Otsuka, A Nakanishi, Y Moriyama, K Takei

    ELECTROPHORESIS   24 ( 18 )   3212 - 3218   2003年9月

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    記述言語:英語   出版者・発行元:WILEY-V C H VERLAG GMBH  

    Exposure of the skin to sunlight results in an increase of the content of epidermal trans-urocanic acid, a key metabolite Of L-histidine, and also in occurrence of the isomerization of trans-urocanic acid to the cis isomer. S-[2-Carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione (GS(CIE)), An adduct of urocanic acid and glutathione, is a presumed origin of a urinary compound S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]-L-cysteine (Cys(CIE)). The formation of GS(CIE) is stimulated by exposing the skin to sunlight irradiation. In this study we investigated an enzymatic formation of GS(CIE) from glutathione and cis-urocanic acid by incubation with rat liver extract that contained glutathione S-transferase (GST) at high activity. The formation of GS(CIE) was suppressed significantly when a liver extract depleted of GST activity was used. Enzymatic degradation of GS(CIE) with gamma-glutamyl transpeptidase resulted in the formation of N-{S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]-L-cysteinyl}glycine, a metabolic intermediate between the glutathione adduct and Cys(CIE). A hydrolyzed product of GS(CIE) by HCl was identical with the urinary Cys(CIE). Compounds were analyzed by high-voltage paper electrophoresis, capillary electrophoresis, and fast atom bombardment mass spectrometry. From these results, we suggest that GS(CIE) formed from cis-urocanic acid and glutathione is an origin of the urinary compound Cys(CIE) and that the formation reaction is catalyzed mostly by the action of GST.

    DOI: 10.1002/elps.200305582

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  • ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type I gamma

    M Krauss, M Kinuta, MR Wenk, P De Camilli, K Takei, Haucke, V

    JOURNAL OF CELL BIOLOGY   162 ( 1 )   113 - 124   2003年7月

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    記述言語:英語   出版者・発行元:ROCKEFELLER UNIV PRESS  

    Clathrin-mediated endocytosis of synaptic vesicle membranes involves the recruitment of clathrin and AP-2 adaptor complexes to the presynaptic plasma membrane. Phosphoinositides have been implicated in nucleating coat assembly by directly binding to several endocytotic proteins including AP-2 and AP180. Here, we show that the stimulatory effect of ATP and GTPgammaS on clathrin coat recruitment is mediated at least in part by increased levels Of PIP2. We also provide evidence for a role of ADP-ribosylation factor 6 (ARF6) via direct stimulation of a synaptically enriched phosphatidylinositol 4-phosphate 5-kinase type Igamma (PIPKIgamma), in this effect. These data suggest a model according to which activation of PIPKIgamma by ARF6-GTP facilitates clathrin-coated pit assembly at the synapse.

    DOI: 10.1083/jcb.200301006

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  • 小胞形成に対するdynamin及びamphiphysinのリン酸化-脱リン酸化の効果

    荒木 健太, 阿部 匡史, 福田 功, 富澤 一仁, 山田 浩司, 須田 城, 絹田 正裕, 竹居 孝二

    日本細胞生物学会大会講演要旨集   56回   52 - 52   2003年5月

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    記述言語:日本語   出版者・発行元:(一社)日本細胞生物学会  

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  • 脳研究の総合的推進に関する研究 神経シナプスにおけるエンドサイトーシスの変化を指標とした神経変性疾患の解析

    竹居孝二, 絹田正裕, 山田浩司

    脳研究の総合的推進に関する研究 平成13年度採択公募班員   2003年

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  • イノシトールリン脂質によるエンドサイトーシスの制御機構

    絹田正裕, 竹居孝二

    生化学   75,1449-1452   2003年

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  • Distribution of dynamins in testis and their possible relation to spermatogenesis

    A Kamitani, H Yamada, M Kinuta, M Watanabe, SA Li, T Matsukawa, M McNiven, H Kumon, K Takei

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   294 ( 2 )   261 - 267   2002年6月

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    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Dynamin 2 and dynamin 3 are highly expressed in testis. However, their functions in the tissue remain unclear. Considering that dynamin 1, neuron-specific isoform of dynamin, plays a pivotal role in endocytosis, functions of dynamin 2 and dynamin 3 in testis must be essential. Cellular expression and subcellular localization of dynamin 2 and dynamin 3 in testis were investigated. Dynamin 2 and dynainin 3 were highly expressed in germ cells and Sertoli cells, constituents of seminiferous tubules. By immunofluorescence it was revealed that dynamin 2 colocalizes with clathrin both at the plasmamembrane and at Golgi in a cell line of Sertoli cells. Immuno reactivity for dynamin 3, on the other hand, appeared as finer puncta, which did not colocalize with clathrin, suggesting that these two dynamins have distinct functions in Sertoli cells. In the klotho deficient mouse testis, which demonstrates disorder in spermatogenesis, expression of dynamin 2 and dynamin 3 was drastically reduced indicating possible association of these proteins with spermatogenesis. (C) 2002 Elsevier Science (USA). All rights reserved.

    DOI: 10.1016/S0006-291X(02)00470-9

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  • Utilization of liposomes in vesicular transport studies

    M Kinuta, K Takei

    CELL STRUCTURE AND FUNCTION   27 ( 2 )   63 - 69   2002年4月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:JAPAN SOC CELL BIOLOGY  

    Various coated vesicles are implicated in the intracellular transport between different compartments. In vitro reconstitution is a powerful experimental system to study molecular mechanisms involved in assembly of coat proteins from cytosol onto membranes as well as formation of coated vesicles. Liposomes have been recently utilized in the cell-free systems. In this review, we summarize studies on reconstitutions of coated vesicles or coated structures on liposomes. A novel method using dynamic tight scattering (DLS) to quantify vesicle formation from liposomes also is described. Our recent study on the role of phospholipids in vesicle formation, where the DSL assay is used in combination with lipid analysis, also is introduced.

    DOI: 10.1247/csf.27.63

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  • Cdk5/p35 regulates neurotransmitter release through phosphorylation and downregulation of P/Q-type voltage-dependent calcium channel activity

    K Tomizawa, J Ohta, M Matsushita, A Moriwaki, ST Li, K Takei, H Matsui

    JOURNAL OF NEUROSCIENCE   22 ( 7 )   2590 - 2597   2002年4月

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    記述言語:英語   出版者・発行元:SOC NEUROSCIENCE  

    Cyclin-dependent kinase 5 (Cdk5) is a proline-directed serine/ threonine kinase with close structural homology to the mitotic Cdks. The complex of Cdk5 and p35, the neuron-specific regulatory subunit of Cdk5, plays important roles in brain development, such as neuronal migration and neurite outgrowth. Moreover, Cdk5 is thought to be involved in the promotion of neurodegeneration in Alzheimer's disease.
    Cdk5 is abundant in mature neurons; however, its physiological functions in the adult brain are unknown. Here we show that Cdk5/p35 regulates neurotransmitter release in the presynaptic terminal. Both Cdk5 and p35 were abundant in the synaptosomes. Roscovitine, a specific inhibitor of Cdk5 in neurons, induced neurotransmitter release from the synaptosomes in response to membrane depolarization and enhanced the EPSP slopes in rat hippocampal slices. The electrophysiological study using each specific inhibitor of the voltage-dependent calcium channels (VDCCs) and calcium imaging revealed that roscovitine enhanced Ca2+ influx from the P/Q-type VDCC. Moreover, Cdk5/p25 phosphorylated the intracellular loop connecting domains II and III (LII-III) between amino acid residues 724 and 981 of isoforms cloned from rat brain of the alpha(1A) subunit of P/Q-type Ca2+ channels. The phosphorylation inhibited the interaction of LII-III with SNAP-25 and synaptotagmin I, which were plasma membrane soluble N-ethylmaleimide- sensitive factor attachment protein (SNAP) receptor (SNARE) proteins and were required for efficient neurotransmitter release. These results strongly suggest that Cdk5/p35 inhibits neurotransmitter release through the phosphorylation of P/Q-type VDCC and downregulation of the channel activity.

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  • Phosphatidylinositol 4,5-bisphosphate stimulates vesicle formation from liposomes by brain cytosol

    M Kinuta, H Yamada, T Abe, M Watanabe, SA Li, A Kamitani, T Yasuda, T Matsukawa, H Kumon, K Takei

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   99 ( 5 )   2842 - 2847   2002年3月

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    記述言語:英語   出版者・発行元:NATL ACAD SCIENCES  

    As a step toward the elucidation of mechanisms in vesicle budding, a cell-free assay that measures cytosol-induced vesicle generation from liposomes was established. This assay then was used to explore the role of phosphoinositides in vesicle formation. Liposomes incubated with brain cytosol in the presence of ATP and GTP massively generated small vesicles, as assessed both quantitatively and qualitatively by a dynamic light-scattering assay. Both ATP and GTP were required. Vesicle formation was inhibited greatly by the immunodepletion of dynamin 1 from the cytosol, indicating a major contribution of this GTPase in this reaction and suggesting that it mimics endocytic vesicle fission. Increasing the concentration Of L-alpha-phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P-2] but not of L-alpha-phosphatidylinositol 4-monophosphate or L-alpha-phosphatidylinositol in the lipid membranes enhanced vesicle formation. Lipid analysis revealed rapid degradation of PtdIns(4,5)P-2 to L-alpha-phosphatidylinositol during the incubation with the reaction reaching a maximum within 5 sec, whereas vesicle formation proceeded with a longer time course. PtdIns(4,5)P-2 degradation was independent of vesicle formation and occurred also in the presence of guanosine 5'-O-(thiotriphosphate), where few vesicle formations occurred. These results suggest that PtdIns(4,5)P-2 plays a critical role in the early step of vesicle formation, possibly in the recruitment of coats and fission factors to membranes.

    DOI: 10.1073/pnas.261715599

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  • ラット培養セルトリ細胞におけるDynamin2,3の発現

    山田浩司, 紙谷章弘, 渡部昌実, 絹田正裕, 公文裕巳, 竹居孝二

    生化学   74 ( 8 )   2002年

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  • 脳及び精巣におけるKlothoタンパクの発現とKlotho変異マウスのエンドサイトーシス機能タンパクの発現変化

    李順愛, 絹田正裕, 紙谷章弘, 紙谷章弘, 山田浩司, 公文裕巳, 竹居孝二

    日本細胞生物学会大会講演要旨集   55th   2002年

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  • Norrpinephrine-evoked 5-hydroxytryptamine secretion is Ca2+ dependent and mediated through exocytosis in rat pinealocytes.

    Yamada H, Hayashi M, Kinoshita M, Uehara S, Watanabe M, Takei K, Moriyama Y

    J Neurochem   2002年

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  • Generation of high curvature membranes mediated by direct endophilin bilayer interactions

    K Farsad, N Ringstad, K Takei, Floyd, SR, K Rose, P De Camilli

    JOURNAL OF CELL BIOLOGY   155 ( 2 )   193 - 200   2001年10月

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    記述言語:英語   出版者・発行元:ROCKEFELLER UNIV PRESS  

    Endophilin 1 is a presynaptically enriched protein which binds the GTPase dynamin and the polyphosphoinositide phosphatase synptojanin. Perturbation of endophilin function in cell-free systems and in a living synapse has implicated endophilin in endocytic vesicle budding (Ringstad, N., H. Gad, P. Low, G. Di Paolo, L. Brodin, O. Shupliakov, and P. De Camilli. 1999. Neuron. 24:143-154; Schmidt, A., M. Wolde, C. Thiele, W. Fest, H. Kratzin, AN. Podtelejnikov, W. Witke, W.B. Huttner, and H.D. Soling. 1999. Nature. 401:133-141; Gad, H., N. Ringstad, P. Low, O. Kjaerulff, J. Gustafsson, M. Wenk, G. Di Paolo, Y. Nemoto, J. Crun, M.H. Ellisman, et al. 2000. Neuron. 27:301-312). Here, we show that purified endophilin can directly bind and evaginate lipid bilayers into narrow tubules similar in diameter to the neck of a clathrin-coated bud, providing new insight into the mechanisms through which endophilin may participate in membrane deformation and vesicle budding. This property of endophilin is independent of its putative lysophosphatydic acid acyl transferase activity, is mediated by its NH2-terminal region, and requires an amino acid stretch homologous to a corresponding region in amphiphysin, a protein previously shown to have similar effects on lipid bilayers (Takei, K., V.I. Slepnev,V. Haucke, and P. De Camilli. 1999. Nat. Cell Biol. 1:33-39). Endophilin cooligomerizes with dynamin rings on lipid tubules and inhibits dynamin's GTP-dependent vesiculating activity. Endophilin B, a protein with homology to endophilin 1, partially localizes to the Golgi complex and also deforms lipid bilayers into tubules, underscoring a potential role of endophilin family members in diverse tubulovesicular membrane-trafficking events in the cell.

    DOI: 10.1083/jcb.200107075

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  • Determination of S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]glutathione, a novel metabolite of L-histidine, in tissue extracts from sunlight-irradiated rat by capillary electrophoresis

    M Kinuta, J Ohta, H Yamada, K Kinuta, T Abe, SA Li, A Otsuka, A Nakanishi, K Takei

    ELECTROPHORESIS   22 ( 16 )   3365 - 3370   2001年10月

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    記述言語:英語   出版者・発行元:WILEY-V C H VERLAG GMBH  

    Exposure of the skin to sunlight results in an increase in the content of epidermal Urocanic acid, a key metabolite Of L-histidine, and some portions of the metabolite penetrate into the body fluid. S-[2-Carboxy-1 -(1H-imidazol-4-yl)ethyl]glutathione (GS(CIE)), an adduct of glutathione and urocanic acid, was proposed to be an origin of a urinary compound, S-[2-carboxy-1-(1H-imidazol-4-yl)ethyl]-L-cysteine (Cys(CIE)). Various catabolites of Cys(CIE) were also isolated from human urine previously. However, no direct evidence to show the existence of GS(CIE) as a biological material had been found. By using capillary electrophoresis, the glutathione adduct has now been found in the extracts of rat tissues from the kidney, liver, skin and blood when the rat was kept under conditions of sunlight irradiation after the fur on the dorsal skin had been clipped. On the other hand, no or a trace of GS(CIE) was determined in rat tissue extracts when the animal was kept indoor in usual manner. The glutathione adduct was isolated from the kidney extract of the sunlight-irradiated rat using ion-exchangers and high-voltage paper electrophoresis, and determined by fast-atom-bombardment mass spectrometry. These results indicate that GS(CIE) formation actually occurs in the body and that the formation is accelerated by exposing the rat to sunlight irradiation. From these findings, we propose an alternative pathway of histidine metabolism which is initiated by the adduction of urocanic acid to glutathione to form GS(CIE) and terminates with the formation of the urinary compounds via Cys(CIE).

    DOI: 10.1002/1522-2683(200109)22:16<3365::AID-ELPS3365>3.0.CO;2-M

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  • Clathrin-mediated endocytosis: membrane factors pull the trigger

    K Takei, Haucke, V

    TRENDS IN CELL BIOLOGY   11 ( 9 )   385 - 391   2001年9月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:ELSEVIER SCIENCE LONDON  

    Clathrin-mediated endocytosis is a vesicular transport event involved in the internalization and recycling of receptors participating in signal transduction events and nutrient import as well as in the reformation of synaptic vesicles. Recent studies in vitro and in living cells have provided a number of new insights into the initial steps of clathrin-coated vesicle formation and the membrane factors involved in this process. The unexpected complexity of these interactions at the cytosol-membrane interface suggests that clathrin-coated vesicle assembly is a highly cooperative process occurring under tight regulatory control. In this review, we focus on the role of membrane proteins and lipids in the nucleation of clathrin-coated pits and provide a hypothetical model for the early steps in clathrin-mediated endocytosis.

    DOI: 10.1016/S0962-8924(01)02082-7

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  • Ptdlns(4,5)P2の分解とエンドサイトーシス

    山田浩司, 絹田正裕, 阿部匡史, 李順愛, 渡部昌美, 紙谷章弘, 公文裕巳, 竹居孝二

    神経化学   40 ( 2/3 )   2001年

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  • エンドサイトーシスにおけるPtdIns(4,5)P2の分解

    絹田正裕, 山田浩司, 阿部匡史, 李順愛, 渡部昌実, 紙谷章弘, 公文裕巳, 竹居孝二

    日本細胞生物学会大会講演要旨集   54th   2001年

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  • ラット培養セルトリ細胞におけるエンドサイトーシス関連蛋白質の発現

    紙谷章弘, 山田浩司, 渡部昌実, 絹田正裕, 公文裕巳, 竹居孝二

    日本細胞生物学会大会講演要旨集   54th   2001年

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  • ラット松果体細胞からのセロトニンの開口放出

    上原俊介, 山田浩司, 林美都子, 木下美香, 渡部昌美, 竹居孝二, 森山芳則

    生化学   73 ( 8 )   2001年

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  • Characterization of synaptojanin 2B isoforms expressed in brain and tests.

    Nemoto Y, Wenk M R, Watanabe M, Daniel L, Murakami T, Nemoto T, Ringstad N, Yamada H, Takei K, De Camilli P

    J Biol Chem   2001年

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  • Interactions of dynamin and amphiphysin with liposomes

    K Takei, Slepnev, VI, P De Camilli

    REGULATORS AND EFFECTORS OF SMALL GTPASES, PT E   329   478 - 486   2001年

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:ACADEMIC PRESS INC  

    DOI: 10.1016/S0076-6879(01)29109-5

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  • 細胞膜のダイナミックス エンドサイトーシスにおける細胞膜脂質の動態

    竹居孝二, 絹田正裕, 阿部匡史, 山田浩司, 太田潤, 渡部昌実

    日本細胞生物学会大会講演要旨集   53rd   2000年

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講演・口頭発表等

  • 細胞操作と定量イメージングで知る細胞骨格ダイナミズム 細胞の形態形成における膜-細胞骨格の動的相互作用

    竹田 哲也, 藤瀬 賢志郎, 山田 浩司, 竹居 孝二

    日本細胞生物学会大会講演要旨集  2020年6月  (一社)日本細胞生物学会

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    開催年月日: 2020年6月

    記述言語:日本語  

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  • 腎糸球体ポドサイトにおけるアンフィファイジン1の機能

    山田 浩司, The Mon La, 竹田 哲也, 阿部 匡史, 淺沼 克彦, 竹居 孝二

    日本生化学会大会プログラム・講演要旨集  2019年9月  (公社)日本生化学会

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    開催年月日: 2019年9月

    記述言語:英語  

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  • 腎糸球体ポドサイトにおけるダイナミンイソフォームの局在と機能

    阿部 匡史, The Mon La, 橘 洋美, 竹田 哲也, 竹居 孝二, 山田 浩司

    日本生化学会大会プログラム・講演要旨集  2019年9月  (公社)日本生化学会

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    開催年月日: 2019年9月

    記述言語:日本語  

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  • 筋細胞膜リモデリングにおけるメカニカルストレス応答の解析

    藤瀬 賢志郎, 山田 浩司, 竹居 孝二, 竹田 哲也

    日本筋学会学術集会プログラム・抄録集  2019年8月  日本筋学会

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    開催年月日: 2019年8月

    記述言語:日本語  

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  • 膜リモデリングおよびリン脂質代謝異常に起因する先天性ミオパチー発症機序の解明

    藤瀬 賢志郎, 背山 佳穂, 山下 恭加, 山田 浩司, 戸井 基道, 竹居 孝二, 竹田 哲也

    日本筋学会学術集会プログラム・抄録集  2018年8月  日本筋学会

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    開催年月日: 2018年8月

    記述言語:日本語  

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  • メカノエンザイム・ダイナミンGTPaseによるアクチン線維束化機構の解析

    山田 浩司, 阿部 匡史, 竹田 哲也, 高島 英造, 森田 将之, 竹居 孝二

    日本生物工学会大会講演要旨集  2018年8月  (公社)日本生物工学会

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    開催年月日: 2018年8月

    記述言語:日本語  

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  • ダイナミン-コルタクチンらせん状複合体の解析 機械的なアクチン線維束形成とアクチン脱重合保護作用

    阿部 匡史, 山田 浩司, 竹田 哲也, 内橋 貴之, 安藤 敏夫, 竹居 孝二

    生命科学系学会合同年次大会  2017年12月  生命科学系学会合同年次大会運営事務局

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    開催年月日: 2017年12月

    記述言語:日本語  

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  • エンドサイトーシス生物学の新展開 ダイナミンによる膜切断過程の動態イメージング

    竹田 哲也, 小財 稔矢, 楊 恵然, 石黒 大輝, 背山 佳穂, 熊谷 祐介, 阿部 匡史, 山田 浩司, 内橋 貴之, 安藤 敏夫, 竹居 孝二

    生命科学系学会合同年次大会  2017年12月  生命科学系学会合同年次大会運営事務局

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    開催年月日: 2017年12月

    記述言語:英語  

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  • GTP加水分解に共役したダイナミン依存的膜切断機構の高速原子間力顕微鏡解析

    竹田 哲也, 石黒 大輝, 楊 恵然, 小財 稔矢, 背山 佳穂, 熊谷 祐介, 山田 浩司, 内橋 貴之, 安藤 敏夫, 竹居 孝二

    日本細胞生物学会大会講演要旨集  2017年5月  (一社)日本細胞生物学会

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    開催年月日: 2017年5月

    記述言語:日本語  

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  • ダイナミン1は微小管の配向を調節しTRPV2の細胞膜移行を制御する

    籔彩夏, 安岡宏樹, 片野坂友紀, 阿部匡史, 竹田哲也, 竹居孝二, 山田浩司

    第62回日本生化学会中国・四国支部例会  2021年9月11日 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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  • ダイナミン2の自己重合と膜との相互作用は糸球体ポドサイトのアクチン制御に重要である

    濱崎英理子, 安岡宏樹, 和木田夏輝, 阿部匡史, 竹田哲也, 竹居孝二, 山田浩司

    第62回日本生化学会中国・四国支部例会  2021年9月11日 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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  • 糸球体ポドサイトからのグルタミン酸の放出と開口放出関連タンパクの探索

    安岡宏樹, 西井尚子, 原田結加, 宮地孝明, 阿部匡史, 竹田哲也, 和田淳, 竹居孝二, 山田浩司

    第62回日本生化学会中国・四国支部例会  2021年9月11日 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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  • Dynamin-Cortactin Ring Complex Physically Bundles Actin Filaments and Protects the Bundle from Depolarization

    IGER International Symposium on Now in actin study: Motor protein research reaching a new stage]  2016年 

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  • 神経細胞成長におけるアクチンダイナミクスの制御(Regulation of actin dynamics in growing neurons)

    10th Cell Transplant Society Congress  2009年 

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  • Mechanism of actin-regulation by dynamin/cortactin complex

    米国細胞生物学会/日本細胞生物学会/理化学研究所 発生・再生科学総合研究センター合同ミーティング  2009年 

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担当授業科目

  • 医学セミナー(テュートリアル) (2021年度) 第1学期  - 火2~3

  • 医学セミナー(チュートリアル) (2021年度) 第1学期  - 火2~3

  • 医学研究インターンシップ (2021年度) 特別  - その他

  • 基礎病態演習 (2021年度) 特別  - その他

  • 生化学 (2021年度) 特別  - その他

  • 生化学 (2021年度) 集中  - その他

  • 生化学・分子医化学実習 (2021年度) 特別  - その他

  • 生化学実習 (2021年度) 特別  - その他

  • 生化学I(演習・実習) (2021年度) 特別  - その他

  • 生化学I(講義・演習) (2021年度) 特別  - その他

  • 生化学II(演習・実習) (2021年度) 特別  - その他

  • 生化学II(講義・演習) (2021年度) 特別  - その他

  • 脳卒中特論 (2021年度) 特別  - その他

  • 脳神経科学演習 (2021年度) 特別  - その他

  • 医学セミナー(テュートリアル) (2020年度) 第1学期  - 火2,火3

  • 医学セミナー(チュートリアル) (2020年度) 第1学期  - 火2,火3

  • 医学研究インターンシップ (2020年度) 特別  - その他

  • 基礎病態演習 (2020年度) 特別  - その他

  • 生化学 (2020年度) 特別  - その他

  • 生化学 (2020年度) 集中  - その他

  • 生化学・分子医化学実習 (2020年度) 特別  - その他

  • 生化学I(演習・実習) (2020年度) 特別  - その他

  • 生化学I(講義・演習) (2020年度) 特別  - その他

  • 生化学II(演習・実習) (2020年度) 特別  - その他

  • 生化学II(講義・演習) (2020年度) 特別  - その他

  • 脳神経科学演習 (2020年度) 通年  - その他

  • 脳神経科学総論 (2020年度) 通年  - その他

▼全件表示