2025/06/20 更新

写真a

サトウ ケイタ
佐藤 恵太
SATO Keita
所属
医歯薬学域 助教
職名
助教
プロフィール
生物が持つ光受容タンパク質の分子特性と生理機能に興味があります。

学位

  • 博士(理学) ( 京都大学 )

研究キーワード

  • 光生物学

  • 視覚

  • ロドプシン

  • 非視覚性光受容

  • 光遺伝学

  • 網膜変性疾患

  • 内分泌

  • オプシン

  • in situ hybridization

研究分野

  • ライフサイエンス / 動物生理化学、生理学、行動学

学歴

  • 京都大学   Graduate School of Science  

    2007年4月 - 2012年3月

      詳細を見る

  • 京都大学   Faculty of Science  

    2003年4月 - 2007年3月

      詳細を見る

経歴

  • 岡山大学医歯薬学総合研究科   細胞組織学分野   助教

    2016年5月 - 現在

      詳細を見る

  • 京都大学   Graduate School of Science   研究員

    2012年4月 - 2016年4月

      詳細を見る

所属学協会

委員歴

  • 日本生物物理学会   分野別専門委員  

    2023年1月 - 現在   

      詳細を見る

    団体区分:学協会

    researchmap

  • 日本生物物理学会   分野別専門委員  

    2021年1月 - 2021年12月   

      詳細を見る

    団体区分:学協会

    researchmap

 

論文

  • A repertoire of visible light-sensitive opsins in the deep-sea hydrothermal vent shrimp Rimicaris hybisae. 査読 国際誌

    Yuya Nagata, Norio Miyamoto, Keita Sato, Yosuke Nishimura, Yuki Tanioka, Yuji Yamanaka, Susumu Yoshizawa, Kuto Takahashi, Kohei Obayashi, Hisao Tsukamoto, Ken Takai, Hideyo Ohuchi, Takahiro Yamashita, Yuki Sudo, Keiichi Kojima

    The Journal of biological chemistry   110291 - 110291   2025年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Unlike terrestrial environments, where humans reside, there is no sunlight in the deep sea. Instead, dim visible light from black-body radiation and bioluminescence illuminates hydrothermal vent areas in the deep sea. A deep-sea hydrothermal vent shrimp, Rimicaris hybisae, is thought to detect this dim light using its enlarged dorsal eye; however, the molecular basis of its photoreception remains unexplored. Here, we characterized the molecular properties of opsins, universal photoreceptive proteins in animals, found in R. hybisae. Transcriptomic analysis identified six opsins: three Gq-coupled opsins, one Opn3, one Opn5, and one peropsin. Functional analysis revealed that five of these opsins exhibited light-dependent G protein activity, while peropsin exhibited the ability to convert all-trans-retinal to 11-cis-retinal like photoisomerases. Notably, all the R. hybisae opsins, including Opn5, convergently show visible light sensitivity (around 457-517 nm), whereas most opsins categorized as Opn5 have been demonstrated to be ultraviolet sensitive. Mutational analysis revealed that the unique visible light sensitivity of R. hybisae Opn5 is achieved through the stabilization of a protonated Schiff base by a counterion residue at position 83 (Asp83), which differs from the position identified in other opsins. These findings suggest that the vent shrimp R. hybisae has adapted its photoreceptive devices to dim, deep-sea hydrothermal light by selectively maintaining a repertoire of visible light-sensitive opsins, including the uniquely tuned Opn5.

    DOI: 10.1016/j.jbc.2025.110291

    PubMed

    researchmap

  • An extensive survey of vertebrate-specific, non-visual opsins identifies a novel subfamily, Q113-Bistable (QB) opsin 査読

    Fuki Gyoja, Keita Sato, Takahiro Yamashita, Takehiro G Kusakabe

    Genome Biology and Evolution   2025年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    Abstract

    A group of non-visual opsins specific to vertebrates is essential to understand evolution of lateral eyes, one of the most prominent innovations in this lineage. Nevertheless, our knowledge of their evolutionary history remains limited. To develop an integrated view of their evolution, we surveyed these non-visual opsins (VA opsin, pinopsin, parapinopsin, parietopsin, and parapinopsin-like) in 451 vertebrate genomes. Through extensive manual curation, we completed a high-quality catalog. We could not find them in 202 mammals, supporting previous reports of their loss. VA opsins are highly conserved among non-mammals. In contrast, other opsin subfamilies experienced more dynamic molecular evolution with many secondary losses. In addition, we found a previously unreported opsin subfamily that we named Q113-Bistable (QB) opsin. We found its orthologs only in several lizards and the tuatara. Nevertheless, QB opsin pseudogenes were discovered in diverse taxa, including ray-finned fishes, indicating its ancient origin. QB opsin, parapinopsin, and parietopsin are extremely prone to be lost in the course of evolution, and loss events involving these opsins seem to occur concomitantly. Furthermore, we demonstrated the spectral properties of QB opsin as a UV-sensitive, bistable photo-pigment. This study provides the first integrated view of the entire evolutionary history of this group of opsins.

    DOI: 10.1093/gbe/evaf032

    researchmap

  • Direct photoreception by pituitary endocrine cells regulates hormone release and pigmentation. 査読 国際誌

    Ayaka Fukuda, Keita Sato, Chika Fujimori, Takahiro Yamashita, Atsuko Takeuchi, Hideyo Ohuchi, Chie Umatani, Shinji Kanda

    Science (New York, N.Y.)   387 ( 6729 )   43 - 48   2025年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The recent discovery of nonvisual photoreceptors in various organs has raised expectations for uncovering their roles and underlying mechanisms. In this work, we identified a previously unrecognized hormone-releasing mechanism in the pituitary of the Japanese rice fish (medaka) induced by light. Ca2+ imaging analysis revealed that melanotrophs, a type of pituitary endocrine cell that secretes melanocyte-stimulating hormone, robustly increase the concentration of intracellular Ca2+ during short-wavelength light exposure. Moreover, we identified Opn5m as the key molecule that drives this response. Knocking out opn5m attenuated melanogenesis by reducing tyrosinase expression in the skin. Our findings suggest a mechanism in which direct reception of short-wavelength light by pituitary melanotrophs triggers a pathway that might contribute to protection from ultraviolet radiation in medaka.

    DOI: 10.1126/science.adj9687

    PubMed

    researchmap

  • What is the identity of Gerota fascia? Histological study with cadavers. 査読 国際誌

    Yasuyuki Kobayashi, Kohei Edamura, Takuya Sadahira, Yusuke Tominaga, Satoshi Katayama, Takehiro Iwata, Shingo Nishimura, Tomoko Kobayashi, Keita Sato, Takaaki Komiyama, Ryusuke Momota, Hideyo Ohuchi, Motoo Araki

    International journal of urology : official journal of the Japanese Urological Association   2024年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES: The advancement of laparoscopic surgery has allowed surgeons to see finer anatomical structures during surgery. As a result, several issues have arisen regarding Gerota fascia that cannot be explained by previous interpretations, such as its various forms observed during surgery. To address these issues, we histologically examined the structure of Gerota fascia. METHODS: Specimens for study were prepared from kidneys with Gerota fascia from four cadavers, and the structure was studied histologically. Its thickness and collagen fiber area ratios were measured using ImageJ and compared to those of the epimysium of the rectus abdominis muscle. RESULTS: Connective tissue that appeared to be Gerota fascia was observed in 26 specimens. Histologically, the basic structure of Gerota fascia was a sandwich-like structure with a thin layer of thick, long collagen fibers in the central layer, and small granular collagen fibers scattered at the edges. However, not all areas observed had a similar structure; eight specimens were composed only of small granular collagen fibers. The average thickness of the Gerota fascia was 466 μm, and the area ratio of collagen was 27.1%. In contrast, the epimysium was much thicker than Gerota fascia, and its collagen fibers were much thicker and denser. CONCLUSIONS: Gerota fascia, unlike the epimysium, was a very thin and fragile layer of collagen fibers, and its structure was diverse. This explains why Gerota fascia was observed in various states during surgery. It is important for surgeons to understand the properties of Gerota fascia and to treat it appropriately.

    DOI: 10.1111/iju.15596

    PubMed

    researchmap

  • Functional diversification process of opsin genes for teleost visual and pineal photoreceptions 査読

    Chihiro Fujiyabu, Fuki Gyoja, Keita Sato, Emi Kawano-Yamashita, Hideyo Ohuchi, Takehiro G. Kusakabe, Takahiro Yamashita

    Cellular and Molecular Life Sciences   81 ( 1 )   2024年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    Abstract

    Most vertebrates have a rhodopsin gene with a five-exon structure for visual photoreception. By contrast, teleost fishes have an intron-less rhodopsin gene for visual photoreception and an intron-containing rhodopsin (exo-rhodopsin) gene for pineal photoreception. Here, our analysis of non-teleost and teleost fishes in various lineages of the Actinopterygii reveals that retroduplication after branching of the Polypteriformes produced the intron-less rhodopsin gene for visual photoreception, which converted the parental intron-containing rhodopsin gene into a pineal opsin in the common ancestor of the Teleostei. Additional analysis of a pineal opsin, pinopsin, shows that the pinopsin gene functions as a green-sensitive opsin together with the intron-containing rhodopsin gene for pineal photoreception in tarpon as an evolutionary intermediate state but is missing in other teleost fishes, probably because of the redundancy with the intron-containing rhodopsin gene. We propose an evolutionary scenario where unique retroduplication caused a “domino effect” on the functional diversification of teleost visual and pineal opsin genes.

    DOI: 10.1007/s00018-024-05461-3

    researchmap

    その他リンク: https://link.springer.com/article/10.1007/s00018-024-05461-3/fulltext.html

▼全件表示

書籍等出版物

  • Vertebrate Photoreceptors: Functional Molecular Bases

    佐藤恵太, 七田芳則( 担当: 分担執筆 ,  範囲: Chapter 1: Evolution and Diversity of Visual Pigments in Connection with Their Functional Differences)

    Springer  2014年3月 

     詳細を見る

MISC

  • Molecular Property, Manipulation, and Potential Use of Opn5 and Its Homologs. 国際誌

    Keita Sato, Hideyo Ohuchi

    Journal of molecular biology   168319 - 168319   2023年10月

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    Animal opsin is a G-protein coupled receptor (GPCR) and binds retinal as a chromophore to form a photopigment. The Opsin 5 (Opn5) group within the animal opsin family comprises a diverse array of related proteins, such as Opn5m, a protein conserved across all vertebrate lineages including mammals, and other members like Opn5L1 and Opn5L2 found in non-mammalian vertebrate genomes, and Opn6 found in non-therian vertebrate genomes, along with Opn5 homologs present in invertebrates. Although these proteins collectively constitute a single clade within the molecular phylogenetic tree of animal opsins, they exhibit markedly distinct molecular characteristics in areas such as retinal binding properties, photoreaction, and G-protein coupling specificity. Based on their molecular features, they are believed to play a significant role in physiological functions. However, our understanding of their precise physiological functions and molecular characteristics is still developing and only partially realized. Furthermore, their unique molecular characteristics of Opn5-related proteins suggest a high potential for their use as optogenetic tools through more specialized manipulations. This review intends to encapsulate our current understanding of Opn5, discuss potential manipulations of its molecular attributes, and delve into its prospective utility in the burgeoning field of animal opsin optogenetics.

    DOI: 10.1016/j.jmb.2023.168319

    PubMed

    researchmap

  • Congenital eye anomalies: More mosaic than thought? 国際誌

    Hideyo Ohuchi, Keita Sato, Munenori Habuta, Hirofumi Fujita, Tetsuya Bando

    Congenital anomalies   59 ( 3 )   56 - 73   2019年5月

     詳細を見る

    記述言語:英語  

    The eye is a sensory organ that primarily captures light and provides the sense of sight, as well as delivering non-visual light information involving biological rhythms and neurophysiological activities to the brain. Since the early 1990s, rapid advances in molecular biology have enabled the identification of developmental genes, genes responsible for human congenital diseases, and relevant genes of mutant animals with various anomalies. In this review, we first look at the development of the eye, and we highlight seminal reports regarding archetypal gene defects underlying three developmental ocular disorders in humans: (1) holoprosencephaly (HPE), with cyclopia being exhibited in the most severe cases; (2) microphthalmia, anophthalmia, and coloboma (MAC) phenotypes; and (3) anterior segment dysgenesis (ASDG), known as Peters anomaly and its related disorders. The recently developed methods, such as next-generation sequencing and genome editing techniques, have aided the discovery of gene mutations in congenital eye diseases and gene functions in normal eye development. Finally, we discuss Pax6-genome edited mosaic eyes and propose that somatic mosaicism in developmental gene mutations should be considered a causal factor for variable phenotypes, sporadic cases, and de novo mutations in human developmental disorders.

    DOI: 10.1111/cga.12304

    PubMed

    researchmap

  • 「光受容体」Opn5が示す多様な分子機能

    佐藤 恵太, 山下 高廣, 大内 淑代, 七田 芳則

    生物物理   59 ( 3 )   132 - 136   2019年

     詳細を見る

    担当区分:筆頭著者   記述言語:日本語   出版者・発行元:一般社団法人 日本生物物理学会  

    <p>Opsins are animal photoreceptor proteins that use retinal as their chromophore (ligand) and are classified into several groups based on sequence similarity. Opn5 forms one phylogenetically separate opsin group and includes three vertebrate-specific subgroups, Opn5m, Opn5L1 and Opn5L2. In this article, we introduce highly diversified functionalities of vertebrate Opn5. Three Opn5 subgroups show characteristic spectral sensitivity, preference for retinal isomers and photoreaction process, and work as bistable photoreceptor, retinal chemoreceptor or unidirectional photoreceptor. Such broad spectrum of functionalities may be attributed to optimization for respective physiological functions.</p>

    DOI: 10.2142/biophys.59.132

    CiNii Article

    CiNii Books

    researchmap

    その他リンク: http://search.jamas.or.jp/link/ui/2019266724

講演・口頭発表等

  • SABER-FISH法による光受容タンパク質オプシン発現細胞の分子組織化学的解析

    佐藤恵太, 大内淑代

    日本解剖学会総会・全国学術集会抄録集(CD-ROM)  2024年 

     詳細を見る

    開催年月日: 2024年

    researchmap

  • マウスハーダー腺形成機構の解明:Fgf10ヘテロ機能喪失マウスの解析

    大内淑代, 佐藤恵太, 池田志織, 皆木瞳

    日本解剖学会総会・全国学術集会抄録集(CD-ROM)  2024年 

     詳細を見る

    開催年月日: 2024年

    researchmap

  • カリウムチャネル遺伝子KCNJ13ノックアウト網膜色素上皮では酸化ストレスにより細胞死を生じる

    大内淑代, 神崎勇希, 神崎勇希, 藤田洋史, 佐藤恵太, 細川海音, 松前洋, 森實祐基, 森實祐基

    日本先天異常学会学術集会プログラム・抄録集  2023年 

     詳細を見る

    開催年月日: 2023年

    researchmap

  • 網膜色素上皮細胞におけるレーバー先天黒内障16型原因遺伝子KCNJ13のノックアウトは酸化ストレス感受性を高め細胞死を誘導する

    藤田洋史, 神崎勇希, 神崎勇希, 佐藤恵太, 細川海音, 松前洋, 森實祐基, 森實祐基, 大内淑代

    日本酸化ストレス学会学術集会プログラム・抄録集  2023年 

     詳細を見る

    開催年月日: 2023年

    researchmap

  • 公共scRNA-seqデータの再解析に基づくメダカ下垂体の組織化学的解析

    佐藤恵太, 大内淑代

    日本下垂体研究会学術集会プログラム・講演要旨集  2023年 

     詳細を見る

    開催年月日: 2023年

    researchmap

▼全件表示

受賞

  • 若手奨励賞

    2018年9月   日本生物物理学会   脊椎動物の光受容体Opn5L1は逆行性・自己再生能をもつ新しいタイプのオプシンである

    佐藤 恵太

     詳細を見る

    受賞区分:国内学会・会議・シンポジウム等の賞 

    researchmap

  • Poster Award

    2016年   Gordon Research Conference: Photosensory Receptors & Signal Transduction   Opn5L1 is a reverse and self-regenerating photoreceptor with light-induced covalent chromophore modification

    佐藤 恵太

     詳細を見る

  • Best Poster Award

    2015年   FASEB Science Research Conference “The Biology and Chemistry of Vision”   Non-mammalian photoreceptor protein Opn5L1 is a photocyclic G protein-coupled receptor

    佐藤 恵太

     詳細を見る

共同研究・競争的資金等の研究

  • 自由遊泳下のオプトジェネティックスを実現し、自らをスクリーニングする魚をつくる

    研究課題/領域番号:24K21962  2024年06月 - 2027年03月

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

    神田 真司, 富原 壮真, 佐藤 恵太

      詳細を見る

    配分額:6500000円 ( 直接経費:5000000円 、 間接経費:1500000円 )

    researchmap

  • Fgf10ハプロ不全の表現型バリエーションを生む転写ノイズ可視化の試み

    研究課題/領域番号:24K09998  2024年04月 - 2027年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    大内 淑代, 佐藤 恵太

      詳細を見る

    配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

    researchmap

  • 魚類眼外組織光受容系の分子・細胞・神経機構の解明

    研究課題/領域番号:23K05850  2023年04月 - 2026年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    佐藤 恵太

      詳細を見る

    配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

    researchmap

  • モデル魚類を用いた網膜変性疾患の研究

    2022年05月 - 2023年04月

    公益財団法人 日本応用酵素協会  成人病の病因・病態の解明に関する研究助成(TMFC) 

      詳細を見る

  • ヒト視床下部におけるオプシン5発現ニューロンの同定と機能解明

    2021年12月 - 2022年11月

    公益財団法人 岡山医学振興会  医学研究助成 

    佐藤恵太

      詳細を見る

    担当区分:研究代表者 

    配分額:1000000円 ( 直接経費:1000000円 )

    researchmap

▼全件表示

 

担当授業科目

  • 人体の構造:入門 (2024年度) 特別  - その他

  • 人体構造学 (2024年度) 集中  - その他

  • 発生学 (2024年度) 特別  - その他

  • 細胞組織学 (2024年度) 特別  - その他

  • 細胞組織学実習 (2024年度) 特別  - その他

▼全件表示