Faculty Profiles - MATSUMOTO Takuya

Papers - MATSUMOTO Takuya

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Co-precipitating calcium phosphate as oral detoxification of cadmium Reviewed

Ahmad Bikharudin, Masahiro Okada, Ping-chin Sung, Takuya Matsumoto

Journal of Hazardous Materials 487 137307 - 137307 2025.4

Optimizing β-TCP with E-rhBMP-2-infused fibrin for vertical bone regeneration in a mouse calvarium model

Kun Zhao, Mitsuaki Ono, Xindi Mu, Ziyi Wang, Shichao Xie, Tomoko Yonezawa, Masahiro Okada, Takuya Matsumoto, Takuo Kuboki, Toshitaka Oohashi

Regenerative Biomaterials 12 2025.1

Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

Abstract

Effective reconstruction of large bone defects, particularly in thickness, remains one of the major challenges in orthopedic and dental fields. We previously produced an Escherichia coli-based industrial-scale GMP-grade recombinant human bone morphogenetic protein-2 (E-rhBMP-2) and showed that the combination of E-rhBMP-2 with beta-tricalcium phosphate (β-TCP/E-rhBMP-2) can effectively promote bone reconstruction. However, the limited mechanical strength and poor morphology retention of β-TCP granules are key points that need optimization to obtain more effective grafts and further expand its clinical applications. Therefore, we combined β-TCP/E-rhBMP-2 with fibrin gel to enhance its mechanical properties and usability for vertical bone regeneration. We investigated the mechanical properties and vertical bone regeneration effects of the materials applied, with or without fibrin containing E-rhBMP-2, in a calvarial defect model in mice. Compression tests were conducted to assess the initial stability of the materials. Scanning electron microscopy and Fourier transform infrared spectroscopy were conducted to characterize the presence of fibrin on the scaffold. After 4 and 12 weeks of implantation, micro-computed tomography and histological and immunofluorescent analyses were performed to assess the morphology and volume of the newly formed bone. The fibrin-containing groups had significantly higher initial mechanical strength and higher ability to maintain their morphology in vivo compared to the counterparts without fibrin. However, fibrin gel alone suppressed the bone formation ability of β-TCP/E-rhBMP-2 whereas the presence of high doses of E-rhBMP-2 in fibrin gel resulted in material resorption and enhanced new bone formation. In conclusion, fibrin gel significantly improved the mechanical strength and surgical manageability of the β-TCP/E-rhBMP-2 scaffold, and the addition of E-rhBMP-2 to the fibrin gel further enhanced the vertical bone regeneration and initial structural integrity of the scaffold.

DOI： 10.1093/rb/rbae144

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Other Link： https://academic.oup.com/rb/article-pdf/doi/10.1093/rb/rbae144/61841803/rbae144.pdf

Long-Term Bonding Performance of One-Bottle vs. Two-Bottle Bonding Agents to Lithium Disilicate Ceramics Reviewed

Masao Irie, Masahiro Okada, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto

Polymers 16 ( 16 ) 2266 - 2266 2024.8

Publishing type：Research paper (scientific journal) Publisher：MDPI AG

The aim of this study was to compare the long-term bonding performance to lithium disilicate (LDS) ceramic between one-bottle and two-bottle bonding agents. Bonding performance was investigated under these LDS pretreatment conditions: with hydrofluoric acid (HF) only, without HF, with a two-bottle bonding agent (Tokuyama Universal Bond II) only. Shear bond strengths between LDS and nine resin cements (both self-adhesive and conventional adhesive types) were measured at three time periods: after one-day water storage (Base), and after 5000 and 20,000 thermocycles (TC 5k and TC 20k respectively). Difference in degradation between one- and two-bottle bonding agents containing the silane coupling agent was compared by high-performance liquid chromatography. With HF pretreatment, bond strengths were not significantly different among the three time periods for each resin cement. Without HF, ESTECEM II and Super-Bond Universal showed significantly higher values than others at TC 5k and TC 20k when treated with the recommended bonding agents, especially at TC 20k. Difference in degradation between one- and two-bottle bonding agents containing the silane coupling agent was compared by high-performance liquid chromatography (HPLC). For both cements, these values at TC 20k were also not significantly different from pretreatment with only Tokuyama Universal Bond II. For LDS, long-term bond durability could be maintained by pretreatment with Tokuyama Universal Bond II instead of the hazardous HF.

DOI： 10.3390/polym16162266

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The cell membrane as biofunctional material for accelerated bone repair. Reviewed International journal

Emi Hatano, Nahid Akhter, Risa Anada, Mitsuaki Ono, Toshitaka Oohashi, Takuo Kuboki, Hiroshi Kamioka, Masahiro Okada, Takuya Matsumoto, Emilio Satoshi Hara

Acta biomaterialia 2024.7

Language：English Publishing type：Research paper (scientific journal)

The cell (plasma) membrane is enriched with numerous receptors, ligands, enzymes, and phospholipids that play important roles in cell-cell and cell-extracellular matrix interactions governing, for instance, tissue development and repair. We previously showed that plasma membrane nanofragments (PMNFs) act as nucleation sites for bone formation in vivo, and induce in vitro mineralization within 1 day. In this study, we optimized the methods for generating, isolating, and applying PMNFs as a cell-free therapeutic to expedite bone defect repair. The PMNFs were isolated from different mouse cell lines (chondrocytes, osteoblasts, and fibroblasts), pre-conditioned, lyophilized, and subsequently transplanted into 2 mm critical-sized calvarial defects in mice (n = 75). The PMNFs from chondrocytes, following a 3-day pre-incubation, significantly accelerated bone repair within 2 weeks, through a coordinated attraction of macrophages, endothelial cells, and osteoblasts to the healing site. In vitro experiments confirmed that PMNFs enhanced cell adhesion. Comparison of the PMNF efficacy with phosphatidylserine, amorphous calcium phosphate (ACP), and living cells confirmed the unique ability of PMNFs to promote accelerated bone repair. Importantly, PMNFs promoted nearly complete integration of the regenerated bone with native tissue after 6 weeks (% non-integrated bone area = 15.02), contrasting with the partial integration (% non-integrated bone area = 56.10; p < 0.01, Student's test) with transplantation of ACP. Vickers microhardness tests demonstrated that the regenerated bone after 6 weeks (30.10 ± 1.75) exhibited hardness similar to native bone (31.07 ± 2.46). In conclusion, this is the first study to demonstrate that cell membrane can be a promising cell-free material with multifaceted biofunctional properties that promote accelerated bone repair. STATEMENT OF SIGNIFICANCE.

DOI： 10.1016/j.actbio.2024.07.037

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Water-Mediated On-Demand Detachable Solid-State Adhesive of Porous Hydroxyapatite for Internal Organ Retractions. Reviewed International journal

Masahiro Okada, Shi Chao Xie, Yusuke Kobayashi, Hiroaki Yanagimoto, Daisuke Tsugawa, Masaru Tanaka, Takayoshi Nakano, Takumi Fukumoto, Takuya Matsumoto

Advanced healthcare materials e2304616 2024.5

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

Novel adhesives for biological tissues offer an advanced surgical approach. Here, the authors report the development and application of solid-state adhesives consisting of porous hydroxyapatite (HAp) biocompatible ceramics as novel internal organ retractors. The operational principles of the porous solid-state adhesives are experimentally established in terms of water migration from biological soft tissues into the pores of the adhesives, and their performance is evaluated on several soft tissues with different hydration states. As an example of practical medical utility, HAp adhesive devices demonstrate the holding ability of porcine livers and on-demand detachability in vivo, showing great potential as internal organ retractors in laparoscopic surgery.

DOI： 10.1002/adhm.202304616

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Lipids and Minerals, Interplay in Biomineralization: Nature's Alchemy Reviewed

Bhingaradiya Nutan, Masahiro Okada, Takuya Matsumoto

Tissue Engineering Part B: Reviews 2024.4

Adaptive enhancement of apatite crystal orientation and Young's modulus under elevated load in rat ulnar cortical bone Reviewed

Jun Wang, Takuya Ishimoto, Tadaaki Matsuzaka, Aira Matsugaki, Ryosuke Ozasa, Takuya Matsumoto, Mikako Hayashi, Hyoung Seop Kim, Takayoshi Nakano

Bone 181 117024 - 117024 2024.4

Mid-infrared passive spectroscopic imaging for visualizing tooth quality Reviewed

So Yamashita, Masahiro Okada, Takuya Matsumoto, Ichiro Ishimaru

Journal of Materials Chemistry B 2024

Solid-state inorganic and metallic adhesives for soft biological tissues Reviewed

Masahiro Okada, Takuya Matsumoto

Japanese Dental Science Review 59 439 - 445 2023.12

Fabrication of initial trabecular bone inspired three-dimensional structure with cell membrane nanofragments Reviewed

Koichi Kadoya, Emilio Satoshi Hara, Masahiro Okada, Yu Yang Jiao, Takayoshi Nakano, Akira Sasaki, Takuya Matsumoto

Regenerative Biomaterials 10 rbac088 2023.12

Investigation on bacterial capture and antibacterial properties of acid-treated Ti surface Reviewed

Haruyuki Aoyagi, Masahiro Okada, Hiroaki Yanagimoto, Takuya Matsumoto

Dental Materials 2023.12

Fabrication of a Fish-Bone-Inspired Inorganic–Organic Composite Membrane Reviewed

YuYang Jiao, Masahiro Okada, Bhingaradiya Nutan, Noriyuki Nagaoka, Ahmad Bikharudin, Randa Musa, Takuya Matsumoto

Polymers 15 ( 20 ) 4190 - 4190 2023.10

Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Biological materials have properties like great strength and flexibility that are not present in synthetic materials. Using the ribs of crucian carp as a reference, we investigated the mechanisms behind the high mechanical properties of this rib bone, and found highly oriented layers of calcium phosphate (CaP) and collagen fibers. To fabricate a fish-rib-bone-mimicking membrane with similar structure and mechanical properties, this study involves (1) the rapid synthesis of plate-like CaP crystals, (2) the layering of CaP–gelatin hydrogels by gradual drying, and (3) controlling the shape of composite membranes using porous gypsum molds. Finally, as a result of optimizing the compositional ratio of CaP filler and gelatin hydrogel, a CaP filler content of 40% provided the optimal mechanical properties of toughness and stiffness similar to fish bone. Due to the rigidity, flexibility, and ease of shape control of the composite membrane materials, this membrane could be applied as a guided bone regeneration (GBR) membrane.

DOI： 10.3390/polym15204190

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歯根膜応用を目指した石灰化dECM膜のin vivo骨結合性評価

鈴木美加, 木村剛, 岡田正弘, 松本卓也, 山田将博, 江草宏, 中村奈緒子, 岸田晶夫

日本歯科理工学会誌 42 ( Special Issue81 ) 18 - 18 2023.3

Preparation of mineralized pericardium by alternative soaking for soft–hard interregional tissue application Reviewed

Mika Suzuki, Tsuyoshi Kimura, Yuta Nakano, Mako Kobayashi, Masahiro Okada, Takuya Matsumoto, Naoko Nakamura, Yoshihide Hashimoto, Akio Kishida

Journal of Biomedical Materials Research Part A 111 ( 2 ) 198 - 208 2023.2

Shear Bond Strength of Resin Luting Materials to Lithium Disilicate Ceramic: Correlation between Flexural Strength and Modulus of Elasticity Reviewed

Masao Irie, Masahiro Okada, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto

Polymers 15 ( 5 ) 1128 - 1128 2023.2

Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

This study investigates the effect of the curing mode (dual-cure vs. self-cure) of resin cements (four self-adhesive and seven conventional cements) on their flexural strength and flexural modulus of elasticity, alongside their shear bond strength to lithium disilicate ceramics (LDS). The study aims to determine the relationship between the bond strength and LDS, and the flexural strength and flexural modulus of elasticity of resin cements. Twelve conventional or adhesive and self-adhesive resin cements were tested. The manufacturer’s recommended pretreating agents were used where indicated. The shear bond strengths to LDS and the flexural strength and flexural modulus of elasticity of the cement were measured immediately after setting, after one day of storage in distilled water at 37 °C, and after 20,000 thermocycles (TC 20k). The relationship between the bond strength to LDS, flexural strength, and flexural modulus of elasticity of resin cements was investigated using a multiple linear regression analysis. For all resin cements, the shear bond strength, flexural strength, and flexural modulus of elasticity were lowest immediately after setting. A clear and significant difference between dual-curing and self-curing modes was observed in all resin cements immediately after setting, except for ResiCem EX. Regardless of the difference of the core-mode condition of all resin cements, flexural strengths were correlated with the LDS surface upon shear bond strengths (R2 = 0.24, n = 69, p < 0.001) and the flexural modulus of elasticity was correlated with them (R2 = 0.14, n = 69, p < 0.001). Multiple linear regression analyses revealed that the shear bond strength was 17.877 + 0.166, the flexural strength was 0.643, and the flexural modulus was (R2 = 0.51, n = 69, p < 0.001). The flexural strength or flexural modulus of elasticity may be used to predict the bond strength of resin cements to LDS.

DOI： 10.3390/polym15051128

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Important roles of odontoblast membrane phospholipids in early dentin mineralization Reviewed International journal

Risa Anada, Emilio Satoshi Hara, Noriyuki Nagaoka, Masahiro Okada, Hiroshi Kamioka, Takuya Matsumoto

Journal of Materials Chemistry B 11 ( 3 ) 657 - 666 2023.1

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Royal Society of Chemistry (RSC)

The objective of this study was to first identify the timing and location of early mineralization of mouse first molar, and subsequently, to characterize the nucleation site for mineral formation...

DOI： 10.1039/d2tb02351b

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Host bone microstructure for enhanced resistance to bacterial infections Reviewed

Ryota Watanabe, Aira Matsugaki, Ozkan Gokcekaya, Ryosuke Ozasa, Takuya Matsumoto, Hiroyuki Takahashi, Hidekazu Yasui, Takayoshi Nakano

Biomaterials Advances 2023

Self-adhesive Devices Made of Titanium for Biological Soft Tissue Reviewed

Masahiro Okada, Takuya Matsumoto

Materia Japan 61 ( 11 ) 760 - 764 2022.11

Does Multifunctional Acrylate’s Addition to Methacrylate Improve Its Flexural Properties and Bond Ability to CAD/CAM PMMA Block? Reviewed International journal

Yukinori Maruo, Kumiko Yoshihara, Masao Irie, Noriyuki Nagaoka, Takuya Matsumoto, Shogo Minagi

Materials 15 ( 21 ) 7564 - 7564 2022.10

Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

This study investigated the effects of a multifunctional acrylate copolymer—Trimethylolpropane Triacrylate (TMPTA) and Di-pentaerythritol Polyacrylate (A-DPH)—on the mechanical properties of chemically polymerized acrylic resin and its bond strength to a CAD/CAM polymethyl methacrylate (PMMA) disk. The methyl methacrylate (MMA) samples were doped with one of the following comonomers: TMPTA, A-DPH, or Trimethylolpropane Trimethacrylate (TMPTMA). The doping ratio ranged from 10 wt% to 50 wt% in 10 wt% increments. The flexural strength (FS) and modulus (FM) of PMMA with and without comonomer doping, as well as the shear bond strength (SBS) between the comonomer-doped PMMA and CAD/CAM PMMA disk, were evaluated. The highest FS (93.2 ± 4.2 MPa) was obtained when doped with 20 wt% of TMPTA. For TMPTMA, the FS decreased with the increase in the doping ratio. For SBS, TMPTA showed almost constant values (ranging from 7.0 to 8.2 MPa) regardless of the doping amount, and A-DPH peaked at 10 wt% doping (8.7 ± 2.2 MPa). TMPTMA showed two peaks at 10 wt% (7.2 ± 2.6 MPa) and 40 wt% (6.5 ± 2.3 MPa). Regarding the failure mode, TMPTMA showed mostly adhesive failure between the CAD/CAM PMMA disk and acrylic resin while TMPTA and A-DPH showed an increased rate of cohesive or mixed failures. Acrylate’s addition as a comonomer to PMMA provided improved mechanical properties and bond strength to the CAD/CAM PMMA disk.

DOI： 10.3390/ma15217564

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Distinct Morphologies of Bone Apatite Clusters in Endochondral and Intramembranous Ossification. Reviewed International journal

Emilio Satoshi Hara, Noriyuki Nagaoka, Masahiro Okada, Takayoshi Nakano, Takuya Matsumoto

Advanced biology 6 ( 11 ) e2200076 2022.7

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

Bone apatite crystals grow in clusters, but the microstructure of these clusters is unknown. This study compares the structural and compositional differences between bone apatite clusters formed in intramembranous (IO) and endochondral ossification (EO). Calvaria (IO) and femurs (EO) are isolated from mice at embryonic days (E) 14.5 to 15.5 and post-natal days (P) 6 to 7, respectively. Results show that the initially formed bone apatite clusters in EO (≅1.2 µm2 ) are >10 times larger than those in IO (≅0.1 µm2 ), without significant changes in ion composition. In IO (E14.5 calvarium), early minerals are formed inside matrix vesicles (MVs). In contrast, in EO (P6 femur epiphysis), no MVs are observed, and chondrocyte-derived plasma membrane nanofragments (PMNFs) are the nucleation site for mineralization. Apatite cluster size difference is linked with the different nucleation sites. Moreover, an alkaline pH and slow P supply into a Ca-rich microenvironment are suggested to facilitate apatite cluster growth, as demonstrated in a biomimetic mineralization system. Together, the results reveal for the first time the distinct and exquisite microstructures of bone apatite clusters in IO and EO, and provide insightful inspirations for the design of more efficient materials for bone tissue engineering and repair.

DOI： 10.1002/adbi.202200076

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A Novel Ex Vivo Bone Culture Model for Regulation of Collagen/Apatite Preferential Orientation by Mechanical Loading Reviewed

Ryota Watanabe, Aira Matsugaki, Takuya Ishimoto, Ryosuke Ozasa, Takuya Matsumoto, Takayoshi Nakano

International Journal of Molecular Sciences 23 ( 13 ) 7423 - 7423 2022.7

Publishing type：Research paper (scientific journal) Publisher：MDPI AG

The anisotropic microstructure of bone, composed of collagen fibers and biological apatite crystallites, is an important determinant of its mechanical properties. Recent studies have revealed that the preferential orientation of collagen/apatite composites is closely related to the direction and magnitude of in vivo principal stress. However, the mechanism of alteration in the collagen/apatite microstructure to adapt to the mechanical environment remains unclear. In this study, we established a novel ex vivo bone culture system using embryonic mouse femurs, which enabled artificial control of the mechanical environment. The mineralized femur length significantly increased following cultivation; uniaxial mechanical loading promoted chondrocyte hypertrophy in the growth plates of embryonic mouse femurs. Compressive mechanical loading using the ex vivo bone culture system induced a higher anisotropic microstructure than that observed in the unloaded femur. Osteocytes in the anisotropic bone microstructure were elongated and aligned along the long axis of the femur, which corresponded to the principal loading direction. The ex vivo uniaxial mechanical loading successfully induced the formation of an oriented collagen/apatite microstructure via osteocyte mechano-sensation in a manner quite similar to the in vivo environment.

DOI： 10.3390/ijms23137423

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In vitro culture of hematopoietic stem cell niche using angiopoietin-1-coupled alginate hydrogel Reviewed

Jae Won Lee, Hyun Seung Kim, Soo-Jeong Yon, Takuya Matsumoto, Sang-Kyung Lee, Kuen Yong Lee

International Journal of Biological Macromolecules 209 1893 - 1899 2022.6

Shear bond strength of resin cement on moist dentin and its relation to the flexural strength of resin cement. Reviewed

Masao Irie, Masahiro Okada, Akio Yoshimoto, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto

Dental materials journal 41 ( 3 ) 429 - 439 2022.5

Language：English Publishing type：Research paper (scientific journal)

We sought to compare the bond strength of resin cement on moist dentin to that on dry dentin, and determine the relationship between the bond strength and flexural strength of resin cement. The water content of the moist and dry dentins was estimated using infrared spectroscopy. Four adhesive and three self-adhesive resin cements were used. At three times of immediately, after one-day storage, and after 20,000 thermocycles (TC 20k), the shear bond strengths were measured. For all resin cements, both the shear bond strength and the flexural strength were the lowest immediately after setting; however, after one day of water storage or TC 20k, these resin cements had the highest values. Regardless of the condition of the dentin surface upon shear bond strength, the flexural strength of each resin cement was correlated with the shear bond strength of the dentin surface.

DOI： 10.4012/dmj.2021-278

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新しい合着用レジン添加型グラスアイオノマーセメント接着強さと曲げ特性

入江正郎, 丸尾幸憲, 西川悟郎, 松本卓也

日本歯科理工学会誌 41 ( Special Issue79 ) 66 - 66 2022.5

新しい合着用レジン添加型グラスアイオノマーセメント接着強さと曲げ特性

入江正郎, 丸尾幸憲, 西川悟郎, 松本卓也

日本歯科理工学会誌 41 ( Special Issue79 ) 66 - 66 2022.5

Mechanotransductive Mechanisms of Biomimetic Hydrogel Cues Modulating Meckel's Cartilage Degeneration Reviewed

Mahmoud Farahat, Emilio S. Hara, Risa Anada, Gulsan A.S. Kazi, Nahid M. Akhter, Takuya Matsumoto

Advanced Biology 2101315 - 2101315 2022.3

Self-adhering implantable device of titanium: Enhanced soft-tissue adhesion by sandblast pretreatment Reviewed

Atsushi Yabe, Masahiro Okada, Emilio Satoshi Hara, Yasuhiro Torii, Takuya Matsumoto

Colloids and Surfaces B: Biointerfaces 211 112283 - 112283 2022.3

Cyclic Pressure-Induced Cytokines from Gingival Fibroblasts Stimulate Osteoclast Activity: Clinical Implications for Alveolar Bone Loss in Denture Wearers Reviewed

Yoshihiro Akashi, Atsuhiro Nagasaki, Hiroko Okawa, Takuya Matsumoto, Takeru Kondo, Hirofumi Yatani, Ichiro Nishimura, Hiroshi Egusa

Journal of Prosthodontic Research 2022

Flexural Property of a Composite Biomaterial in Three Applications Reviewed

Masao Irie, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto

JOURNAL OF COMPOSITES SCIENCE 5 ( 10 ) 2021.10

新規開発の仮封材 I級窩洞辺縁部適合性と圧縮強さ

入江正郎, 丸尾幸憲, 西川悟郎, 松本卓也

日本歯科理工学会誌 40 ( Special Issue78 ) 40 - 40 2021.9

前歯用CAD/CAMブロックに対するレジンセメントの接着強さの検討 Self-cure vs.Dual-cure

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

接着歯学 39 ( 3 ) 77 - 77 2021.9

人工歯周組織ユニット創製のためのアパタイトコーティング法の開発

岡田正弘, 木村剛, 中村奈緒子, 岸田晶夫, 松本卓也

日本歯科理工学会誌 40 ( Special Issue78 ) 51 - 51 2021.9

Relationships between Flexural and Bonding Properties, Marginal Adaptation, and Polymerization Shrinkage in Flowable Composite Restorations for Dental Application. International journal

Akimasa Tsujimoto, Masao Irie, Erica Cappelletto Nogueira Teixeira, Carlos Alberto Jurado, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto, Franklin Garcia-Godoy

Polymers 13 ( 16 ) 2021.8

Language：English Publishing type：Research paper (scientific journal)

To evaluate the flexural and bonding properties, marginal adaptation, and polymerization shrinkage in flowable composite restorations and their relationships, four new generation flowable composites, one conventional, and one bulk-fill flowable composite were used in this study. Flexural properties of the composites and shear bond strength to enamel and dentin for flowable restorations were measured immediately and 24 h after polymerization. Marginal adaptation, polymerization shrinkage, and stress were also investigated immediately after polymerization. The flexural properties, and bond strength of the flowable composites to enamel and dentin were much lower immediately after polymerization than at 24 h, regardless of the type of the composite. Polymerization shrinkage and stress varied depending on the material, and bulk-fill flowable composite showed much lower values than the others. The marginal adaptation and polymerization shrinkage of the composites appeared to have a much stronger correlation with a shear bond strength to dentin than to enamel. The weak mechanical properties and bond strengths of flowable composites in the early stage after polymerization must be taken into account when using them in the clinic. In addition, clinicians should be aware that polymerization shrinkage of flowable composites can still lead to the formation of gaps and failure of adaptation to the cavity regardless of the type of composite.

DOI： 10.3390/polym13162613

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Immediate soft-tissue adhesion and the mechanical properties of the Ti–6Al–4V alloy after long-term acid treatment Reviewed International journal

Yaming Wang, Masahiro Okada, Shi Chao Xie, Yu Yang Jiao, Emilio Satoshi Hara, Hiroaki Yanagimoto, Takumi Fukumoto, Takuya Matsumoto

Journal of Materials Chemistry B 9 ( 39 ) 8348 - 8354 2021.8

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Royal Society of Chemistry (RSC)

A metallic solid-state adhesive for biological soft tissues was fabricated using Ti–6Al–4V alloys, and the influence of the minor β phase and the small amount of Al in the α phase are reported.

DOI： 10.1039/d1tb00919b

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2ステップボンディング材の初期接着強さの経時的変化

矢部淳, 入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 154回 99 - 99 2021.5

レジンセメントの最近の歯科用セラミックスに対する接着強さの長期耐久性

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 154回 86 - 86 2021.5

Re-Evaluation of Initial Bone Mineralization from an Engineering Perspective Reviewed

Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takayoshi Nakano, Takuya Matsumoto

Tissue Engineering Part B: Reviews 28 ( 1 ) 246 - 255 2021.3

Coating method for smooth and reinforced surface layer of highly translucent zirconia Reviewed

Yoshiro FUJII, Masahiro OKADA, Hiroaki TAKETA, Takuya MATSUMOTO

Dental Materials Journal 40 ( 2 ) 532 - 537 2021.3

Bone Mineral Analogue Ceramic Block as an Instant Adhesive to Biological Soft Tissue (Adv. Mater. Interfaces 6/2021) Reviewed

Yuki Sugiura, Masahiro Okada, Ken Hirano, Takuya Matsumoto

Advanced Materials Interfaces 8 ( 6 ) 2170033 - 2170033 2021.3

グラスアイオノマーの唾液汚染による歯質接着強さの特徴コンポジットレジンシステムとの比較

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

日本歯科理工学会誌 40 ( Special Issue77 ) 22 - 22 2021.3

サンドブラスト処理による酸処理チタンの軟組織接着性の増強

矢部淳, 岡田正弘, 武田宏明, ハラ・エミリオ・サトシ, 鳥井康弘, 松本卓也

日本歯科理工学会誌 40 ( Special Issue77 ) 3 - 3 2021.3

Effect of Biomechanical Environment on Degeneration of Meckel’s Cartilage Reviewed

M. Farahat, G.A.S. Kazi, E.S. Hara, T. Matsumoto

Journal of Dental Research 100 ( 2 ) 171 - 178 2021.2

Authorship：Last author,　Corresponding author Publishing type：Research paper (scientific journal) Publisher：SAGE Publications

During orofacial tissue development, the anterior and posterior regions of the Meckel’s cartilage undergo mineralization, while the middle region undergoes degeneration. Despite the interesting and particular phenomena, the mechanisms that regulate the different fates of Meckel’s cartilage, including the effects of biomechanical cues, are still unclear. Therefore, the purpose of this study was to systematically investigate the course of Meckel’s cartilage during embryonic development from a biomechanical perspective. Histomorphological and biomechanical (stiffness) changes in the Meckel’s cartilage were analyzed from embryonic day 12 to postnatal day 0. The results revealed remarkable changes in the morphology and size of chondrocytes, as well as the occurrence of chondrocyte burst in the vicinity of the mineralization site, an often-seen phenomenon preceding endochondral ossification. To understand the effect of biomechanical cues on Meckel’s cartilage fate, a mechanically tuned 3-dimensional hydrogel culture system was used. At the anterior region, a moderately soft environment (10-kPa hydrogel) promoted chondrocyte burst and ossification. On the contrary, at the middle region, a more rigid environment (40-kPa hydrogel) enhanced cartilage degradation by inducing a higher expression of MMP-1 and MMP-13. These results indicate that differences in the biomechanical properties of the surrounding environment are essential factors that distinctly guide the mineralization and degradation of Meckel’s cartilage and would be valuable tools for modulating in vitro cartilage and bone tissue engineering.

DOI： 10.1177/0022034520960118

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Other Link： http://journals.sagepub.com/doi/full-xml/10.1177/0022034520960118

Flexural Property of Composite Biomaterials Used in the Oral Cavity Reviewed

Masao Irie, Yukinori Maruo, Goro Nishigawa, Takuya Matsumoto

Preprints 2021 2021.2

Nanostructural analysis of distinct nucleation sites in pathological mineralization Reviewed

Emilio Satoshi Hara, Susumu Oozawa, Noriyuki Nagaoka, Masahiro Okada, Shingo Kasahara, Takuya Matsumoto

Materials Advances 2 ( 13 ) 4423 - 4431 2021

Authorship：Last author,　Corresponding author Publishing type：Research paper (scientific journal) Publisher：Royal Society of Chemistry (RSC)

<p>Vesicular and non-vesicular phospholipids were the nucleation sites in atherosclerotic calcification. Mineralization <italic>in vitro</italic> showed that LDL, PS and PC mineralized in 2 days. PS mineralized more than PC.</p>

DOI： 10.1039/d1ma00273b

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Large three-dimensional cell constructs for tissue engineering. International journal

Jun-Ichi Sasaki, Gabriela L Abe, Aonan Li, Takuya Matsumoto, Satoshi Imazato

Science and technology of advanced materials 22 ( 1 ) 571 - 582 2021

Language：English Publishing type：Research paper (scientific journal)

Much research has been conducted on fabricating biomimetic biomaterials in vitro. Tissue engineering approaches are often conducted by combining cells, scaffolds, and growth factors. However, the degradation rate of scaffolds is difficult to control and the degradation byproducts occasionally limit tissue regeneration. To overcome these issues, we have developed a novel system using a thermo-responsive hydrogel that forms scaffold-free, three-dimensional (3D) cell constructs with arbitrary size and morphology. 3D cell constructs prepared using bone marrow-derived stromal stem cells (BMSCs) exhibited self-organizing ability and formed bone-like tissue with endochondral ossification. Endothelial cells were then introduced into the BMSC construct and a vessel-like structure was formed within the constructs. Additionally, the bone formation ability was promoted by endothelial cells and cell constructs could be freeze-dried to improve their clinical application. A pre-treatment with specific protein protectant allowed for the fabrication of novel bone substitutes composed only of cells. This 3D cell construct technology using thermo-responsive hydrogels was then applied to other cell species. Cell constructs composed of dental pulp stem cells were fabricated, and the resulting construct regenerated pulp-like tissue within a human pulpless tooth. In this review, we demonstrate the approaches for the in vitro fabrication of bone and dental pulp-like tissue using thermo-responsive hydrogels and their potential applications.

DOI： 10.1080/14686996.2021.1945899

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Flexural Strength of Resin Core Build-Up Materials: Correlation to Root Dentin Shear Bond Strength and Pull-Out Force. Reviewed International journal

Masao Irie, Yukinori Maruo, Goro Nishigawa, Kumiko Yoshihara, Takuya Matsumoto

Polymers 12 ( 12 ) 2020.12

Language：English Publishing type：Research paper (scientific journal)

The aims of this study were to investigate the effects of root dentin shear bond strength and pull-out force of resin core build-up materials on flexural strength immediately after setting, after one-day water storage, and after 20,000 thermocycles. Eight core build-up and three luting materials were investigated, using 10 specimens (n = 10) per subgroup. At three time periods-immediately after setting, after one-day water storage, and after 20,000 thermocycles, shear bond strengths to root dentin and pull-out forces were measured. Flexural strengths were measured using a 3-point bending test. For all core build-up and luting materials, the mean data of flexural strength, shear bond strength and pull-out force were the lowest immediately after setting. After one-day storage, almost all the materials yielded their highest results. A weak, but statistically significant, correlation was found between flexural strength and shear bond strength (r = 0.508, p = 0.0026, n = 33). As the pull-out force increased, the flexural strength of core build-up materials also increased (r = 0.398, p = 0.0218, n = 33). Multiple linear regression analyses were conducted using these three independent factors of flexural strength, pull-out force and root dentin shear bond strength, which showed this relationship: Flexural strength = 3.264 × Shear bond strength + 1.533 × Pull out force + 10.870, p = 0.002). For all the 11 core build-up and luting materials investigated immediately after setting, after one-day storage and after 20,000 thermocycles, their shear bond strengths to root dentin and pull-out forces were correlated to the flexural strength in core build-up materials. It was concluded that the flexural strength results of the core build-up material be used in research and quality control for the predictor of the shear bond strength to the root dentin and the retentive force of the post.

DOI： 10.3390/polym12122947

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Micro-Architectural Investigation of Teleost Fish Rib Inducing Pliant Mechanical Property Reviewed

Yu Yang Jiao, Masahiro Okada, Emilio Satoshi Hara, Shi Chao Xie, Noriyuki Nagaoka, Takayoshi Nakano, Takuya Matsumoto

Materials 13 ( 22 ) 5099 - 5099 2020.11

Authorship：Last author,　Corresponding author Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Despite the fact that various reports have been discussing bone tissue regeneration, precise bone tissue manipulation, such as controlling the physical properties of the regenerated bone tissue, still remains a big challenge. Here, we focused on the teleost fish ribs showing flexible and tough mechanical properties to obtain a deeper insight into the structural and functional features of bone tissue from different species, which would be valuable for the superior design of bone-mimicking materials. Herein, we examined their compositions, microstructure, histology, and mechanical properties. The first rib of Carassius langsdorfii showed a higher Young’s modulus with a small region of chondrocyte clusters compared with other smaller ribs. In addition, highly oriented collagen fibers and osteocytes were observed in the first rib, indicating that the longest first rib would be more mature. Moreover, the layer-by-layer structure of the oriented bone collagen was observed in each rib. These microarchitectural and compositional findings of fish rib bone would give one the useful idea to reproduce such a highly flexible rib bone-like material.

DOI： 10.3390/ma13225099

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新規ボンディング材の歯質接着性からの検討湿潤vs.乾燥

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 153回 59 - 59 2020.11

汚染除去材が接着性レジンセメントのせん断接着強さに及ぼす影響

矢部淳, 入江正郎, 岡田正弘, 野崎高儀, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 153回 60 - 60 2020.11

新規レジンセメントの歯質接着性からの検討各種処理材の影響

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 153回 58 - 58 2020.11

新規セルフアドヒーシヴ・レジンセメントの接着性からの検討プライマー併用効果、Self-cure vs.Dual-cure

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

接着歯学 38 ( 3 ) 111 - 111 2020.9

Cellular Fragments as Biomaterial for Rapid In Vitro Bone-Like Tissue Synthesis Reviewed

Mst Nahid Akhter, Emilio Satoshi Hara, Koichi Kadoya, Masahiro Okada, Takuya Matsumoto

International Journal of Molecular Sciences 21 ( 15 ) 5327 - 5327 2020.7

Authorship：Last author,　Corresponding author Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Current stem cell-based techniques for bone-like tissue synthesis require at least two to three weeks. Therefore, novel techniques to promote rapid 3D bone-like tissue synthesis in vitro are still required. In this study, we explored the concept of using cell nanofragments as a substrate material to promote rapid bone formation in vitro. The methods for cell nanofragment fabrication were ultrasonication (30 s and 3 min), non-ionic detergent (triton 0.1% and 1%), or freeze-dried powder. The results showed that ultrasonication for 3 min allowed the fabrication of homogeneous nanofragments of less than 150 nm in length, which mineralized surprisingly in just one day, faster than the fragments obtained from all other methods. Further optimization of culture conditions indicated that a concentration of 10 mM or 100 mM of β-glycerophosphate enhanced, whereas fetal bovine serum (FBS) inhibited in a concentration-dependent manner, the mineralization of the cell nanofragments. Finally, a 3D collagen-cell nanofragment-mineral complex mimicking a bone-like structure was generated in just two days by combining the cell nanofragments in collagen gel. In conclusion, sonication for three min could be applied as a novel method to fabricate cell nanofragments of less than 150 nm in length, which can be used as a material for in vitro bone tissue engineering.

DOI： 10.3390/ijms21155327

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唾液汚染除去材処理がセラミックスの接着強さに与える影響

萬田陽介, 入江正郎, 丸尾幸憲, 西川悟郎, 吉原久美子, 長岡紀幸, 松本卓也, 皆木省吾

日本補綴歯科学会誌 12 ( 特別号 ) 171 - 171 2020.6

ヒト血漿汚染被着面に対する汚染除去材の効果接着強さからの検討

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 152回 95 - 95 2020.6

臼歯修復用手動練和型のグラスアイオノマーセメント I級窩洞適合性と歯質接着強さ

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

日本歯科理工学会誌 39 ( Special Issue75 ) 17 - 17 2020.4

単斜晶ジルコニアとシリカを焼付した高透光性ジルコニアの機械的性質

岡田正弘, 宇野聡一郎, 武田宏明, 鳥井康弘, 松本卓也

日本歯科理工学会誌 39 ( Special Issue75 ) 7 - 7 2020.4

骨質指標としての骨基質配向化制御におけるオステオカルシンの役割

中野貴由, 小笹良輔, 岡田正弘, 松本卓也, 森石武史, 小守壽文

日本歯科理工学会誌 39 ( Special Issue75 ) 10 - 10 2020.4

Toughening of highly translucent zirconia by monoclinic ZrO₂ and SiO₂ particle coating Reviewed

Soichiro UNO, Masahiro OKADA, Hiroaki TAKETA, Yasuhiro TORII, Takuya MATSUMOTO

Dental Materials Journal 39 ( 2 ) 295 - 301 2020.3

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society for Dental Materials and Devices

The mechanical properties of highly translucent partially stabilized zirconia (PSZ) need to be improved; however, improvement of mechanical properties often decreases translucency. To overcome this problem, a monoclinic ZrO2 (mZrO2)/SiO2 dispersion was prepared and applied as a coating material for PSZ. The influence of surface treatment by the mZrO2/SiO2 dispersion on the surface topography, crystallography, and mechanical properties of highly translucent PSZ was investigated in this study. Following the treatment, the mechanical strength of highly translucent PSZ improved by 170% compared with control, for the best mZrO2/SiO2 dispersion ratio and heating temperature condition, while maintaining its translucency. The proposed coating is promising for improving the mechanical properties of highly translucent PSZ.

DOI： 10.4012/dmj.2018-415

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Titanium as an Instant Adhesive for Biological Soft Tissue Reviewed

Masahiro Okada, Emilio Satoshi Hara, Atsushi Yabe, Kei Okada, Yo Shibata, Yasuhiro Torii, Takayoshi Nakano, Takuya Matsumoto

ADVANCED MATERIALS INTERFACES 2020.3

Vibratory splint therapy for decreasing sleep clenching: A pilot study Reviewed

Emilio Satoshi Hara, Andrea Lusvarghi Witzel, Hajime Minakuchi, Carlos Eduardo de Pitta, Rosane Tronquin Gallo, Masahiro Okada, Takuya Matsumoto, Takuo Kuboki, Marcelo Costa Bolzan

CRANIO® 38 ( 1 ) 15 - 21 2020.1

Analysis of bone regeneration based on the relationship between the orientations of collagen and apatite in mouse femur Reviewed

Ryosuke Ozasa, Mayuko Nakatsu, Atsushi Moriguchi, Kyohei Sasaki, Takuya Ishimoto, Masahiro Okada, Takuya Matsumoto, Takayoshi Nakano

Materials Transactions 61 ( 2 ) 381 - 386 2020

Flexural properties, bond ability, and crystallographic phase of highly translucent multi-layered zirconia

Yukinori Maruo, Kumiko Yoshihara, Masao Irie, Goro Nishigawa, Noriyuki Nagaoka, Takuya Matsumoto, Shogo Minagi

Journal of Applied Biomaterials and Functional Materials 18 2020

唾液汚染被着面に対する汚染除去材の効果接着強さからの検討

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 151回 69 - 69 2019.10

Fibronectin‐induced ductal formation in salivary gland self‐organization model Reviewed

Mahmoud Farahat, Gulsan A. S. Kazi, Hiroaki Taketa, Emilio S. Hara, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

Developmental Dynamics 248 ( 9 ) 813 - 825 2019.9

新しい練和方式の合着用レジン添加型グラスアイオノマーセメント接着強さと曲げ特性

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

日本歯科理工学会誌 38 ( Special Issue74 ) 22 - 22 2019.9

セルフアドヒーシヴ・レジンセメント(自動練和型)の歯質接着性からの検討プライマー併用効果、Dual-cure vs.Self-cure

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

接着歯学 37 ( 3 ) 123 - 123 2019.9

Biomimetic mineralization using matrix vesicle nanofragments. Reviewed International journal

Yosuke Kunitomi, Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takuo Kuboki, Takayoshi Nakano, Hiroshi Kamioka, Takuya Matsumoto

Journal of biomedical materials research. Part A 107 ( 5 ) 1021 - 1030 2019.5

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate bone tissue are still ineffective due to some remaining gaps in the understanding of real in vivo bone formation process, and application of the knowledge in bone synthesis. Therefore, the objectives of this study were first, to perform a systematic and ultrastructural investigation of the initial mineral formation during intramembranous ossification of mouse calvaria from a material scientists' viewpoint, and to develop novel mineralization methods based on the in vivo findings. First, the very initial mineral deposition was found to occur at embryonic day E14.0 in mouse calvaria. Analysis of the initial bone formation process showed that it involved the following distinct steps: collagen secretion, matrix vesicle (MV) release, MV mineralization, MV rupture, and collagen fiber mineralization. Next, we performed in vitro mineralization experiments using MVs and hydrogel scaffolds. Intact MVs embedded in collagen gel did not mineralize, whereas, interestingly, MV nanofragments obtained by ultrasonication could promote rapid mineralization. These results indicate that mechanically ruptured MV membrane can be a promising material for in vitro bone tissue synthesis. © 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1021-1030, 2019.

DOI： 10.1002/jbm.a.36618

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最近の歯科用セラミックスに対する接着強さ

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 吉原久美子, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 150回 63 - 63 2019.5

Improvement of mechanical properties of Y-TZP by thermal annealing with monoclinic zirconia nanoparticle coating. Reviewed International journal

Okada M, Taketa H, Hara ES, Torii Y, Irie M, Matsumoto T

Dental materials : official publication of the Academy of Dental Materials 35 ( 7 ) 970 - 978 2019.4

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

OBJECTIVE: To assess whether a thermal annealing with a monoclinic zirconia (mZrO2) nanoparticle coating can improve the reliability of sandblasted yttria-stabilized tetragonal zirconia polycrystals (Y-TZP) and maintain its mechanical strength. METHODS: Commercially available Y-TZP (Lava Frame, 3M Dental Products) disks were sintered and surface-treated as follows: AS (as sintered, with no treatment); SB (sandblasting); SB-TA (sandblasting followed by thermal annealing at 1000 °C); and SB-mZr-TA (sandblasting followed by thermal annealing at 1000 °C with the mZrO2 nanoparticle coating). The mZrO2 nanoparticles of 21 nm in size were prepared by a hydrothermal method, and coated onto Y-TZP sintered disks as a 5 g/L ethanol dispersion. Biaxial flexural strength (S) was measured using the piston-on-three-ball test, and reliability was evaluated by the Weibull modulus (m). RESULTS: Biaxial flexural tests showed a significant increase in the strength of Group SB (SSB = 1445 ± 191 MPa) compared with Group AS (SAS = 1071 ± 112 MPa). The thermal annealing improved the reliabilities of the sandblasted Y-TZP (mSB-TA = 20.14 and mSB-mZr-TA = 21.33), as compared with Group SB (mSB = 7.77). However, the conventional thermal annealing without the mZrO2 coating caused a significant decrease in the strength of sandblasted Y-TZP (SSB-TA = 1273 ± 65 MPa). Importantly, the mZrO2 coating prevented the decrease in the strength caused by conventional thermal annealing (SSB-mZr-TA = 1379 ± 65 MPa). SIGNIFICANCE: The thermal annealing with the mZrO2 nanoparticle coating can improve the reliability of sandblasted Y-TZP and maintain its mechanical strength, which would otherwise be decreased by the conventional annealing process.

DOI： 10.1016/j.dental.2019.04.002

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単斜晶ナノジルコニア焼付による従来型ジルコニアの機械的性質改善

岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

日本歯科理工学会誌 38 ( Special Issue73 ) 8 - 8 2019.4

Fabricating large-scale three-dimensional constructs with living cells by processing with syringe needles. International journal

Jun-Ichi Sasaki, Chihiro Katata, Gabriela L Abe, Takuya Matsumoto, Satoshi Imazato

Journal of biomedical materials research. Part A 107 ( 4 ) 904 - 909 2019.4

Language：English Publishing type：Research paper (scientific journal)

Three-dimensional (3D) cell constructs composed only of cells and cell-secreted extracellular matrix have been attractive biomaterials for tissue engineering technology; however, controlling construct morphology and eliminating dead cells after fabrication remain a challenge. It has been hypothesized that moderate stress could shape constructs and eliminate dead cells. The purpose of this study was to establish an easily available technology for shaping 3D cell constructs and eliminating dead cells postfabrication. To achieve these objectives, spherical cell constructs composed of L-929 fibroblasts were processed using different sized syringe needles. Our results revealed that large-scale rod-shaped cell constructs could be fabricated, and that their diameters could be controlled according to the size of the syringe needle. Additionally, cell viability assays showed that >94% of cells in the rod-shaped constructs were viable, suggesting that dead cells, which have low adhesion force, were dispersed when compressive stress was applied during passage through the needle. The technology described in this study will be promising for future tissue engineering, especially for fabricating elongated tissues such as nerves and blood vessels. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 904-909, 2019.

DOI： 10.1002/jbm.a.36613

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新しい器械式練和の充填用グラスアイオノマーセメント I級窩洞適合性と歯質接着強さ

入江正郎, 丸尾幸憲, 西川悟郎, 皆木省吾, 松本卓也

日本歯科理工学会誌 38 ( Special Issue73 ) 62 - 62 2019.4

Intermediate Water on Calcium Phosphate Minerals: Its Origin and Role in Crystal Growth

Masahiro Okada, Emilio Satoshi Hara, Daisuke Kobayashi, Shoki Kai, Keiko Ogura, Masaru Tanaka, Takuya Matsumoto

ACS Applied Bio Materials 2 981 - 986 2019.3

Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells Reviewed

Yuri Hazehara-Kunitomo, Emilio Hara, Mitsuaki Ono, Kyaw Thu Aung, Keiko Komi, Hai Thanh Pham, Kentaro Akiyama, Masahiro Okada, Toshitaka Oohashi, Takuya Matsumoto, Takuo Kuboki

International Journal of Molecular Sciences 20 ( 5 ) 2019.3

Optimal sandblasting conditions for conventional-type yttria-stabilized tetragonal zirconia polycrystals Reviewed International journal

Okada Masahiro, Taketa Hiroaki, Torii Yasuhiro, Irie Masao, Matsumoto Takuya

DENTAL MATERIALS 35 ( 1 ) 169 - 175 2019.1

Fabrication of single gel with different mechanical stiffness using three-dimensional mold Reviewed

Kazi Gulsan, Ara Sathi, Rahman Kazi Anisur, Farahat Mahmoud, Matsumoto Takuya

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 107 ( 1 ) 6 - 11 2019.1

新規表面処理法が高透光性ジルコニアの機械的性質および接着性に及ぼす影響

武田宏明, 岡田正弘, 宇野聡一郎, 入江正郎, 鳥井康弘, 松本卓也

岡山歯学会雑誌 37 ( 2 ) 84 - 84 2018.12

最近のフロアブルコンポジットの重合挙動と窩洞適合性

入江正郎, 岡田正弘, 武田宏明, 鳥井康弘, 吉原久美子, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 149回 64 - 64 2018.10

高透光性ジルコニアに対する機械的表面処理条件の検討

岡田正弘, 藤井惠朗, 宇野聡一郎, 武田宏明, 鳥井康弘, 松本卓也

日本歯科理工学会誌 37 ( Special Issue72 ) 35 - 35 2018.9

ワンステップ接着システムにおける歯質界面の生成物と物性の関係

鈴木一臣, 長岡紀幸, 吉原久美子, 松本卓也, 吉田靖弘

日本歯科理工学会誌 37 ( Special Issue72 ) 29 - 29 2018.9

下顎偏位時における舌の三次元的位置解析

徳善貴大, 兒玉直紀, 長塚弘亮, 村上明日香, 田中祐貴, 松本卓也, 皆木省吾

日本補綴歯科学会誌 10 ( 特別号 ) 260 - 260 2018.6

レジンセメントの大臼歯用CAD/CAM Blockに対するせん断接着強さ

入江正郎, 武田宏明, 鳥井康弘, 吉原久美子, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 148回 61 - 61 2018.5

高透光性ジルコニアの機械的強さに及ぼす表面処理の影響

大島直也, 岡田正弘, 宇野聡一郎, 武田宏明, 鳥井康弘, 松本卓也

日本歯科理工学会誌 37 ( Special Issue71 ) 38 - 38 2018.3

Bioinspired Mineralization Using Chondrocyte Membrane Nanofragments

Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

ACS Biomaterials Science & Engineering 4 617 - 625 2018.2

Bioinspired Mineralization Using Chondrocyte Membrane Nanofragments Reviewed

Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

ACS Biomaterials Science & Engineering 4 ( 2 ) 617 - 625 2018.2

Chondrocyte burst promotes space for mineral expansion Reviewed

Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Letycia Mary Iida, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

Integrative Biology 10 ( 1 ) 57 - 66 2018

Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu

Emilio Satoshi Hara, Masahiro Okada, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

JOURNAL OF MATERIALS CHEMISTRY B 6 ( 38 ) 6153 - 6161 2018

Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu Reviewed

Emilio Satoshi Hara, Masahiro Okada, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

Journal of Materials Chemistry B 6 ( 38 ) 6153 - 6161 2018

Fabrication of Hydroxyapatite Nanofibers with High Aspect Ratio via Low-Temperature Wet Precipitation Methods Under Acidic Conditions

Masahiro Okada, Emilio Satoshi Hara, Takuya Matsumoto

Biomineralization 211 - 218 2018

The effect of Mg and Sr on the crystallinity of bones evaluated through Raman spectroscopy and laser ablation-ICPMS analysis Reviewed

Yu-ki Tanaka, Nobuyuki Yajima, Masahiro Okada, Takuya Matsumoto, Yusuke Higuchi, Shigeaki Miyazaki, Hideyuki Yamato, Takafumi Hirata

ANALYST 142 ( 22 ) 4265 - 4278 2017.11

Resin adhesion strengths to zirconia ceramics after primer treatment with silane coupling monomer or oligomer Reviewed

Masahiro Okada, Kazusa Inoue, Masao Irie, Hiroaki Taketa, Yasuhiro Torii, Takuya Matsumoto

DENTAL MATERIALS JOURNAL 36 ( 5 ) 600 - 605 2017.10

歯根象牙質と直接法レジンコアー材料との接着性耐久性

西川悟郎, 入江正郎, 丸尾幸憲, 徳永英里, 吉原久美子, 長岡紀幸, 松本卓也, 皆木省吾

接着歯学 35 ( 3 ) 79 - 79 2017.10

最近のフロアブルコンポジットの歯質接着性と曲げ特性

入江正郎, 徳永英里, 丸尾幸憲, 西川悟郎, 吉原久美子, 長岡紀幸, 皆木省吾, 松本卓也

接着歯学 35 ( 3 ) 77 - 77 2017.10

ファイバーポストとコアー材の引き抜き強さ

入江正郎, 武田宏明, 鳥井康弘, 吉原久美子, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 147回 148 - 148 2017.10

歯科用ジルコニアへのサンドブラスト処理の影響

岡田正弘, 武田宏明, 鳥井康弘, 入江正郎, 松本卓也

日本歯科理工学会誌 36 ( 5 ) 327 - 327 2017.9

Biocompatible nanostructured solid adhesives for biological soft tissues Reviewed

Masahiro Okada, Akira Nakai, Emilio Satoshi Hara, Tetsushi Taguchi, Takayoshi Nakano, Takuya Matsumoto

Acta Biomaterialia 57 404 - 413 2017.7

ファイバーポストとコアー材の初期の引き抜き強さ

入江正郎, 武田宏明, 鳥井康弘, 吉原久美子, 松本卓也

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 146回 91 - 91 2017.5

MSCs feeder layers induce SMG self-organization and branching morphogenesis Reviewed

Mahmoud Farahat, Gulsan Ara Sathi, Emilio Satoshi Hara, Hiroaki Taketa, Takuo Kuboki, Takuya Matsumoto, Masaya Yamamoto

PLOS ONE 12 ( 4 ) e0176453 2017.4

Does 8-methacryloxyoctyl trimethoxy silane(8-MOTS) improve initial bond strength on lithium disilicate glass ceramic? Reviewed

Yukinori Maruo, Goro Nishigawa, Kumiko Yoshihara, Shogo Minagi, Takuya Matsumoto, Masao Irie

DENTAL MATERIALS 33 ( 3 ) E95 - E100 2017.3

MCSF orchestrates branching morphogenesis in developing submandibular gland tissue Reviewed

Gulsan Ara Sathi, Mahmoud Farahat, Emilio Satoshi Hara, Hiroaki Taketa, Hitoshi Nagatsuka, Takuo Kuboki, Takuya Matsumoto

Journal of Cell Science 130 ( 9 ) 1559 - 1569 2017.3

ジルコニアの曲げ強さに与える表面処理の影響

岡田正弘, 武田宏明, 鳥井康弘, 松本卓也

日本歯科理工学会誌 36 ( 2 ) 67 - 67 2017.3

Does acid etching morphologically and chemically affect lithium disilicate glass ceramic surfaces? Reviewed

Yukinori Maruo, Goro Nishigawa, Masao Irie, Kumiko Yoshihara, Takuya Matsumoto, Shogo Minagi

JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS 15 ( 1 ) E93 - E100 2017.1

Fabrication of Artificial Food Bolus for Evaluation of Swallowing Reviewed

Miyu Hosotsubo, Tetsuro Magota, Masahiko Egusa, Takuya Miyawaki, Takuya Matsumoto

PLOS ONE 11 ( 12 ) e0168378 2016.12

最近の合着用セメント象牙質とCAD/CAM用レジンブロックに対する接着強さ

入江正郎, 飯田祥与, 丸尾幸憲, 西川悟郎, 皆木省吾, 長岡紀幸, 吉原久美子, 松本卓也

接着歯学 34 ( 4 ) 141 - 149 2016.12

新規動揺歯固定材の歯質接着性と曲げ特性

入江正郎, 飯田祥与, 丸尾幸憲, 西川悟郎, 吉原久美子, 長岡紀幸, 皆木省吾, 松本卓也

接着歯学 34 ( 3 ) 117 - 117 2016.11

プライマーの併用が可能な新規セルフアドヒーシブ・レジンセメントの歯質接着性と曲げ特性

西川悟郎, 飯田祥与, 丸尾幸憲, 入江正郎, 吉原久美子, 長岡紀幸, 皆木省吾, 松本卓也

接着歯学 34 ( 3 ) 113 - 113 2016.11

Adsorption and desorption behaviors of cetylpyridinium chloride on hydroxyapatite nanoparticles with different morphologies Reviewed

Masahiro Okada, Daisuke Hiramatsu, Takumi Okihara, Takuya Matsumoto

DENTAL MATERIALS JOURNAL 35 ( 4 ) 651 - 658 2016.8

Highly dynamic self-organizing SMG morphogenesis in 3D culture Reviewed

Mahmoud Farahat, Gulsan Ara Sathi, Hiroaki Taketa, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015 1 - 3 2016.3

Functional peptide KP24 enhances submandibular gland tissue growth in vitro

Atsushi Ikeda, Hiroaki Taketa, Gulsan Ara Sathi, Yoshiaki Hirano, Seiji Iida, Takuya Matsumoto

REGENERATIVE THERAPY 3 108 - 113 2016.3

Various Effects of Sandblasting of Dental Restorative Materials Reviewed

Goro Nishigawa, Yukinori Maruo, Masao Irie, Naoto Maeda, Kumiko Yoshihara, Noriyuki Nagaoka, Takuya Matsumoto, Shogo Minagi

PLOS ONE 11 ( 1 ) e0147077 2016.1

Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells Reviewed

Hiroko Okawa, Hiroki Kayashima, Jun-Ichi Sasaki, Jiro Miura, Yuya Kamano, Yukihiro Kosaka, Satoshi Imazato, Hirofumi Yatani, Takuya Matsumoto, Hiroshi Egusa

STEM CELLS INTERNATIONAL 2016 6240794 2016

Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells Reviewed

Hiroko Okawa, Hiroki Kayashima, Jun-Ichi Sasaki, Jiro Miura, Yuya Kamano, Yukihiro Kosaka, Satoshi Imazato, Hirofumi Yatani, Takuya Matsumoto, Hiroshi Egusa

Stem Cells International 2016 2016

レジンセメントのCAD/CAM用レジンブロックに対する接着強さに及ぼすサンドブラスト処理の影響

入江正郎, 田仲持郎, 松本卓也, 丸尾幸憲, 西川悟郎, 皆木省吾, 吉原久美子

接着歯学 33 ( 4 ) 181 - 186 2015.12

咀嚼による食材の物性変化と嚥下との関係

細坪充裕, 江草正彦, 宮脇卓也, 松本卓也

日本摂食・嚥下リハビリテーション学会雑誌 19 ( 3 ) S287 - S287 2015.12

Synthesis and modification of apatite nanoparticles for use in dental and medical applications Reviewed

Masahiro Okada, Takuya Matsumoto

Japanese Dental Science Review 51 ( 4 ) 85 - 95 2015.11

新規動揺歯固定および矯正用ブラケット接着材のエナメル質に対する接着強さ

入江正郎, 田仲持郎, 松本卓也, 丸尾幸憲, 西川悟郎, 皆木省吾, 吉原久美子, 長岡紀幸

接着歯学 33 ( 3 ) 143 - 143 2015.11

最近のレジンセメントの歯質接着性と曲げ特性

丸尾幸憲, 入江正郎, 西川悟郎, 吉原久美子, 長岡紀幸, 皆木省吾, 松本卓也

接着歯学 33 ( 3 ) 142 - 142 2015.11

Fluocinolone acetonideとTGF-β3を用いた強力な軟骨再生(Potent cartilage regeneration with fluocinolone acetonide and TGF-β3)

Hara Emilio Satoshi, 大野充昭, Pham Hai Thanh, 久保田聡, 滝川正春, Young Marian F., Olsen Bjorn R., 松本卓也, 窪木拓男

日本バイオマテリアル学会大会予稿集 37回 209 - 209 2015.11

充填用グラスアイオノマーセメント I級窩洞適合性と歯質接着強さ

入江正郎, 田仲持郎, 松本卓也, 武田宏明, 鳥井康弘, 丸尾幸憲, 西川悟郎, 皆木省吾, 長岡紀幸, 吉原久美子

日本歯科理工学会誌 34 ( 5 ) 407 - 407 2015.9

Peptide-modified Substrate for Modulating Gland Tissue Growth and Morphology In Vitro. Reviewed International journal

Hiroaki Taketa, Gulsan Ara Sathi, Mahmoud Farahat, Kazi Anisur Rahman, Takayoshi Sakai, Yoshiaki Hirano, Takuo Kuboki, Yasuhiro Torii, Takuya Matsumoto

Scientific reports 5 11468 - 11468 2015.6

Early Initiation of Endochondral Ossification of Mouse Femur Cultured in Hydrogel with Different Mechanical Stiffness Reviewed

Gulsan Ara Sathi, Kodai Kenmizaki, Satoshi Yamaguchi, Hitoshi Nagatsuka, Yasuhiro Yoshida, Aira Matsugaki, Takuya Ishimoto, Satoshi Imazato, Takayoshi Nakano, Takuya Matsumoto

TISSUE ENGINEERING PART C-METHODS 21 ( 6 ) 567 - 575 2015.6

Fabrication of Biomimetic Bone Tissue Using Mesenchymal Stem Cell-Derived Three-Dimensional Constructs Incorporating Endothelial Cells Reviewed

Jun-Ichi Sasaki, Masanori Hashimoto, Satoshi Yamaguchi, Yoshihiro Itoh, Itsumi Yoshimoto, Takuya Matsumoto, Satoshi Imazato

PLOS ONE 10 ( 6 ) e0129266 2015.6

Early Initiation of Endochondral Ossification of Mouse Femur Cultured in Hydrogel with Different Mechanical Stiffness Reviewed

Gulsan Ara Sathi, Kodai Kenmizaki, Satoshi Yamaguchi, Hitoshi Nagatsuka, Yasuhiro Yoshida, Aira Matsugaki, Takuya Ishimoto, Satoshi Imazato, Takayoshi Nakano, Takuya Matsumoto

TISSUE ENGINEERING PART C-METHODS 21 ( 6 ) 567 - 575 2015.6

Biodental engineering Reviewed

Takuya Matsumoto, Hiroshi Egusa, Koichi Kato, Takashi Tsuji

Journal of Oral Biosciences 57 ( 2 ) 80 - 85 2015.5

Biodental engineering Reviewed

Matsumoto Takuya, Egusa Hiroshi, Kato Koichi, Tsuji Takashi

JOURNAL OF ORAL BIOSCIENCES 57 ( 2 ) 80 - 85 2015.5

CAD/CAMレジンブロックとレジンセメントの歯質接着性に関する研究

飯田祥与, 入江正郎, 西川悟郎, 丸尾幸憲, 吉原久美子, 前田直人, 荒木大介, 萬田陽介, 松本卓也, 皆木省吾

日本補綴歯科学会誌 7 ( 特別号 ) 263 - 263 2015.5

1液性ボンディング材中に含まれるシランカップリング材のカップリング効果の検討

吉原久美子, 長岡紀幸, 入江正郎, 西川悟郎, 丸尾幸憲, 松本卓也, 皆木省吾, 吉田靖弘

日本補綴歯科学会誌 7 ( 特別号 ) 169 - 169 2015.5

Novel 3D-Printed Device for the Simple Preparation of Hydrogel Beads

Kazi Anisur Rahman, Gulsan Ara Sathi, Hiroaki Taketa, Mahmoud Farahat, Masahiro Okada, Yasuhiro Torii, Takuya Matsumoto

3D PRINTING AND ADDITIVE MANUFACTURING 2 ( 1 ) 5 - 11 2015.3

Novel 3D-Printed Device for the Simple Preparation of Hydrogel Beads Reviewed

Kazi Anisur Rahman, Gulsan Ara Sathi, Hiroaki Taketa, Mahmoud Farahat, Masahiro Okada, Yasuhiro Torii, Takuya Matsumoto

3D PRINTING AND ADDITIVE MANUFACTURING 2 ( 1 ) 5 - 11 2015.3

充填用グラスアイオノマーセメントの初期の歯質接着強さと曲げ特性コーティング材塗布の効果

入江正郎, 田仲持郎, 松本卓也, 武田宏明, 鳥井康弘, 丸尾幸憲, 西川悟郎, 皆木省吾, 長尾紀幸

日本歯科理工学会誌 34 ( 2 ) 81 - 81 2015.3

Oriented bone formation using biomimetic fibrin hydrogels with three-dimensional patterned bone matrices Reviewed

Jun-Ichi Sasaki, Takuya Matsumoto, Satoshi Imazato

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 103 ( 2 ) 622 - 627 2015.2

Fluocinolone Acetonide Is a Potent Synergistic Factor of TGF-beta 3-Associated Chondrogenesis of Bone Marrow-Derived Mesenchymal Stem Cells for Articular Surface Regeneration Reviewed

Hara, Emilio Satoshi, Ono, Mitsuaki, Hai Thanh Pham, Sonoyama, Wataru, Kubota, Satoshi, Takigawa, Masaharu, Matsumoto, Takuya, Young, Marian F., Olsen, Bjorn R., Kuboki, Takuo

Journal of Bone and Mineral Research 30 ( 9 ) 1585 - 1596 2015

Antagonistic Effects of Insulin and TGF-beta 3 during Chondrogenic Differentiation of Human BMSCs under a Minimal Amount of Factors Reviewed

Hara, Emilio Satoshi, Ono, Mitsuaki, Yoshioka, Yuya, Ueda, Junji, Hazehara, Yuri, Hai Thanh Pham, Matsumoto, Takuya, Kuboki, Takuo

Cells Tissues Organs 201 ( 2 ) 88 - 96 2015

Ultra-structural evaluation of an anodic oxidated titanium dental implant Reviewed

Akiyoshi Yamagami, Noriyuki Nagaoka, Kumiko Yoshihara, Mariko Nakamura, Hajime Shirai, Takuya Matsumoto, Kazuomi Suzuki, Yasuhiro Yoshida

DENTAL MATERIALS JOURNAL 33 ( 6 ) 828 - 834 2014.12

ニケイ酸リチウムガラスとレジンセメントの接着シラン処理材の影響

丸尾幸憲, 西川悟郎, 入江正郎, 吉原久美子, 長岡紀幸, 松本卓也, 皆木省吾

接着歯学 32 ( 3 ) 175 - 175 2014.11

セルフアドヘッシブ・レジンセメントの劣化加速試験後の機械的特性

入江正郎, 田仲持郎, 松本卓也, 長岡紀幸, 丸尾幸憲, 西川悟郎, 皆木省吾, 吉山昌宏

接着歯学 32 ( 3 ) 166 - 166 2014.11

マウス大腿骨の二次骨化中の組織石灰化に関する定性分析(Qualitative analysis of tissue mineralization during secondary ossification of mouse femur)

ハラ・エミリオ・サトシ, 岡田正弘, 服部高子, 久保田聡, 窪木拓男, 松本卓也

日本バイオマテリアル学会大会予稿集 36回 251 - 251 2014.11

Comparative Analysis of Mouse-Induced Pluripotent Stem Cells and Mesenchymal Stem Cells During Osteogenic Differentiation In Vitro Reviewed

Hiroshi Egusa, Hiroki Kayashima, Jiro Miura, Shinya Uraguchi, Fangfang Wang, Hiroko Okawa, Jun-Ichi Sasaki, Makio Saeki, Takuya Matsumoto, Hirofumi Yatani

STEM CELLS AND DEVELOPMENT 23 ( 18 ) 2156 - 2169 2014.9

充填用グラスアイオノマーセメントの硬化初期の歯質接着強さと機械的強さ

入江正郎, 田仲持郎, 松本卓也, 武田宏明, 鳥井康弘, 丸尾幸憲, 西川悟郎, 長岡紀幸

日本歯科理工学会誌 33 ( 5 ) 483 - 483 2014.9

コンポジットレジン修復の歯質接着強さの経時的推移

入江正郎, 田仲持郎, 松本卓也, 丸尾幸憲, 西川悟郎, 吉山昌宏

接着歯学 32 ( 1 ) 8 - 13 2014.4

合着用レジン配合型グラスアイオノマーセメントの硬化初期の接着性と曲げ特性

入江正郎, 田仲持郎, 松本卓也, 武田宏明, 鳥井康弘, 丸尾幸憲, 西川悟郎, 長岡紀幸

日本歯科理工学会誌 33 ( 2 ) 153 - 153 2014.3

二官能メタクリレートの添加が即時重合レジンの重合収縮に与える影響

山本美恵, 丸尾幸憲, 入江正郎, 西川悟郎, 松本卓也, 皆木省吾

日本歯科理工学会誌 33 ( 2 ) 121 - 121 2014.3

miRNA-720 regulates the stem cell phenotype and differentiation of human dental pulp-derived mesenchymal stromal cells Reviewed

Hara, Emilio Satoshi, Ono, Mitsuaki, Eguchi, Takanori, Hai Thanh Pham, Tajima, Shoji, Calderwood, Stuart K., Matsumoto, Takuya, Kuboki, Takuo, IEEE

2014 International Symposium on Micro-Nanomechatronics and Human Science (Mhs) 1 - 2 2014

1P176 Mapping elastic modulus of mouse submandibular gland tissue by atomic force microscopy(12. Cell biology,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Nakamura Mitsuhiro, Fujii Yuki, Taketa Hiroaki, Matsumoto Takuya, Okajima Takaharu

Seibutsu Butsuri 54 ( 1 ) S170 2014

セルフアドヘッシヴ・レジンセメントのサーマルサイクル負荷後のジルコニアに対する接着強さと曲げ特性

入江正郎, 田仲持郎, 松本卓也, 長岡紀幸, 丸尾幸憲, 西川悟郎, 吉山昌宏

接着歯学 31 ( 3 ) 139 - 139 2013.11

最近のセルフアドヘッシヴ・レジンセメントの硬化初期における接着特性

丸尾幸憲, 西川悟郎, 入江正郎, 長岡紀幸, 松本卓也, 皆木省吾

接着歯学 31 ( 3 ) 138 - 138 2013.11

ジルコニア表面に対するリン酸モノマー処理の検討

長岡紀幸, 玉田宜之, 入江正郎, 吉原久美子, 吉田靖弘, 西川悟郎, 丸尾幸憲, 松本卓也, 皆木省吾, 早川聡

日本歯科理工学会誌 32 ( 5 ) 354 - 354 2013.9

合着用レジン添加型グラスアイオノマーセメントの種々の被着体への接着性と曲げ特性サーマルサイクルの影響

入江正郎, 田仲持郎, 松本卓也, 丸尾幸憲, 西川悟郎, 吉山昌宏

接着歯学 31 ( 2 ) 45 - 52 2013.8

Application of Cyclic Strain for Accelerated Skeletal Myogenic Differentiation of Mouse Bone Marrow-Derived Mesenchymal Stromal Cells with Cell Alignment Reviewed

Hiroshi Egusa, Munemasa Kobayashi, Takuya Matsumoto, Jun-Ichi Sasaki, Shinya Uraguchi, Hirofumi Yatani

TISSUE ENGINEERING PART A 19 ( 5-6 ) 770 - 782 2013.3

Application of Cyclic Strain for Accelerated Skeletal Myogenic Differentiation of Mouse Bone Marrow-Derived Mesenchymal Stromal Cells with Cell Alignment Reviewed

Hiroshi Egusa, Munemasa Kobayashi, Takuya Matsumoto, Jun-Ichi Sasaki, Shinya Uraguchi, Hirofumi Yatani

TISSUE ENGINEERING PART A 19 ( 5-6 ) 770 - 782 2013.3

二ケイ酸リチウムガラスセラミックスの接着強さにシランカップリング剤分子中の加水分解基数が及ぼす影響

西川悟郎, 入江正郎, 丸尾幸憲, 山本美恵, 玉田宜之, 前田直人, 長岡紀幸, 松本卓也, 皆木省吾

日本歯科理工学会誌 32 ( 2 ) 154 - 154 2013.3

ロカテック処理したジルコニア表面の断面TEM観察

玉田宜之, 長岡紀幸, 武田宏明, 吉原久美子, 入江正郎, 西川悟郎, 丸尾幸憲, 吉田靖弘, 鳥井康弘, 松本卓也, 皆木省吾

日本歯科理工学会誌 32 ( 2 ) 152 - 152 2013.3

Phosphorylated Pullulan Bioadhesive for Regeneration and Reconstruction of Bone and Tooth Reviewed

Yasuhiro Yoshida, Takumi Okihara, Mariko Nakamura, Takuya Matsumoto

BIOCERAMICS 24 529-530 516 - + 2013

In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO2: HA formation, cellular affinity and organ culture

Sang-Hyun An, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroyuki Miyajima, Ramaswamy Narayanan, Satoshi Imazato, Kyo-Han Kim

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 32 ( 8 ) 2516 - 2522 2012.12

Porous zirconia/hydroxyapatite scaffolds for bone reconstruction Reviewed

Sang-Hyun An, Takuya Matsumoto, Hiroyuki Miyajima, Atsushi Nakahira, Kyo-Han Kim, Satoshi Imazato

DENTAL MATERIALS 28 ( 12 ) 1221 - 1231 2012.12

In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO2: HA formation, cellular affinity and organ culture Reviewed

Sang-Hyun An, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroyuki Miyajima, Ramaswamy Narayanan, Satoshi Imazato, Kyo-Han Kim

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 32 ( 8 ) 2516 - 2522 2012.12

合着用レジン添加型グラスアイオノマーセメントの歯質接着性と曲げ特性

入江正郎, 田仲持郎, 松本卓也, 玉田宜之, 丸尾幸憲, 西川悟郎, 吉山昌宏

接着歯学 30 ( 4 ) 137 - 144 2012.12

HEMA Inhibits Interfacial Nano-layering of the Functional Monomer MDP

Y. Yoshida, K. Yoshihara, S. Hayakawa, N. Nagaoka, T. Okihara, T. Matsumoto, S. Minagi, A. Osaka, K. Van Landuyt, B. Van Meerbeek

JOURNAL OF DENTAL RESEARCH 91 ( 11 ) 1060 - 1065 2012.11

新しい多用途型処理材の接着強さからの検討

入江正郎, 田仲持郎, 松本卓也, 玉田宜之, 丸尾幸憲, 西川悟郎, 吉山昌宏

接着歯学 30 ( 3 ) 126 - 126 2012.11

汚染されたジルコニア表面に対するリン酸エステルによるカップリング処理

長岡紀幸, 玉田宜之, 入江正郎, 西川悟郎, 丸尾幸憲, 吉田靖弘, 松本卓也

接着歯学 30 ( 3 ) 114 - 114 2012.11

ジルコニアに対するリン酸モノマーの吸着特性

玉田宜之, 長岡紀幸, 早川聡, 入江正郎, 西川悟郎, 丸尾幸憲, 吉田靖弘, 松本卓也, 尾坂明義, 皆木省吾

日本歯科理工学会誌 31 ( 5 ) 479 - 479 2012.9

二官能メタクリレートの添加が即時重合レジンの曲げ特性に与える影響

山本美恵, 丸尾幸憲, 入江正郎, 西川悟郎, 玉田宜之, 松本卓也, 皆木省吾

日本歯科理工学会誌 31 ( 5 ) 452 - 452 2012.9

即時重合レジンの重合収縮の計測方法に関する研究

丸尾幸憲, 山本美恵, 入江正郎, 西川悟郎, 玉田宜之, 松本卓也, 皆木省吾

日本歯科理工学会誌 31 ( 5 ) 458 - 458 2012.9

Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis

Kenichi Harimoto, Yasuhiro Yoshida, Kumiko Yoshihara, Noriyuki Nagaoka, Takuya Matsumoto, Yoh-ichi Tagawa

DENTAL MATERIALS JOURNAL 31 ( 4 ) 674 - 680 2012.7

X-ray diffraction analysis of three-dimensional self-reinforcing monomer and its chemical interaction with tooth and hydroxyapatite Reviewed

Yasuhiro Yoshida, Kumiko Yoshihara, Noriyuki Nagaoka, Masao Hanabusa, Takuya Matsumoto, Yasuko Momoi

DENTAL MATERIALS JOURNAL 31 ( 4 ) 697 - 702 2012.7

Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis Reviewed

Kenichi Harimoto, Yasuhiro Yoshida, Kumiko Yoshihara, Noriyuki Nagaoka, Takuya Matsumoto, Yoh-ichi Tagawa

DENTAL MATERIALS JOURNAL 31 ( 4 ) 674 - 680 2012.7

Surface characterization of alkali- and heat-treated Ti with or without prior acid etching

Sang-Hyun An, Takuya Matsumoto, Hiroyuki Miyajima, Jun-Ichi Sasaki, Ramaswamy Narayanan, Kyo-Han Kim

APPLIED SURFACE SCIENCE 258 ( 10 ) 4377 - 4382 2012.3

ジルコニア表面のリン酸モノマーによる有機化の検討

玉田宜之, 長岡紀幸, 早川聡, 入江正郎, 西川悟郎, 丸尾幸憲, 吉田靖弘, 松本卓也, 尾坂明義, 皆木省吾

日本歯科理工学会誌 31 ( 2 ) 190 - 190 2012.3

In vitro reproduction of endochondral ossification using a 3D mesenchymal stem cell construct Reviewed

Jun-Ichi Sasaki, Takuya Matsumoto, Hiroshi Egusa, Michiya Matsusaki, Akihiro Nishiguchi, Takayoshi Nakano, Mitsuru Akashi, Satoshi Imazato, Hirofumi Yatani

INTEGRATIVE BIOLOGY 4 ( 10 ) 1207 - 1214 2012

Zirconia-hydroxyapatite composite material with micro porous structure Reviewed

Takuya Junior Matsumoto, Sang-Hyun An, Takuya Ishimoto, Takayoshi Nakano, Takuya Matsumoto, Satoshi Imazato

DENTAL MATERIALS 27 ( 11 ) E205 - E212 2011.11

Zirconia-hydroxyapatite composite material with micro porous structure

Takuya Junior Matsumoto, Sang-Hyun An, Takuya Ishimoto, Takayoshi Nakano, Takuya Matsumoto, Satoshi Imazato

DENTAL MATERIALS 27 ( 11 ) E205 - E212 2011.11

Hydrogel-based biomimetic environment for in vitro modulation of branching morphogenesis Reviewed

Hiroyuki Miyajima, Takuya Matsumoto, Takayoshi Sakai, Satoshi Yamaguchi, Sang Hyun An, Makoto Abe, Satoshi Wakisaka, Kuen Yong Lee, Hiroshi Egusa, Satoshi Imazato

BIOMATERIALS 32 ( 28 ) 6754 - 6763 2011.10

Crystallinity of Anodic TiO(2) Nanotubes and Bioactivity

Sang-Hyun An, Ramaswamy Narayanan, Takuya Matsumoto, Hyo-Jin Lee, Tae-Yub Kwon, Kyo-Han Kim

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 11 ( 6 ) 4910 - 4918 2011.6

Cell-Based Fabrication of Organic/Inorganic Composite Gel Material Reviewed

Takuya Matsumoto, Ami Mizuno, Miki Kashiwagi, Shin-suke Yoshida, Jun-ichi Sasaki, Takayoshi Nakano

MATERIALS 4 ( 1 ) 327 - 338 2011.1

Modulation of nanotube formation in apatite single crystal via organic molecule incorporation

Matsumoto, T., Uddin, M.H., An, S.H., Arakawa, K., Taguchi, E., Nakahira, A., Okazaki, M.

Materials Chemistry and Physics 128 ( 3 ) 455 - 499 2011

Gingival Fibroblasts as a Promising Source of Induced Pluripotent Stem Cells Reviewed

Hiroshi Egusa, Keisuke Okita, Hiroki Kayashima, Guannan Yu, Sho Fukuyasu, Makio Saeki, Takuya Matsumoto, Shinya Yamanaka, Hirofumi Yatani

PLOS ONE 5 ( 9 ) e12743 2010.9

Fabrication of Three-Dimensional Cell Constructs Using Temperature-Responsive Hydrogel Reviewed

Jun-Ichi Sasaki, Taka-Aki Asoh, Takuya Matsumoto, Hiroshi Egusa, Taiji Sohmura, Eben Alsberg, Mitsuru Akashi, Hirofumi Yatani

TISSUE ENGINEERING PART A 16 ( 8 ) 2497 - 2504 2010.8

Effect of Calcium Ion Concentrations on Osteogenic Differentiation and Hematopoietic Stem Cell Niche-Related Protein Expression in Osteoblasts Reviewed

Shinya Nakamura, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroshi Egusa, Kuen Yong Lee, Takayoshi Nakano, Taiji Sohmura, Atsushi Nakahira

TISSUE ENGINEERING PART A 16 ( 8 ) 2467 - 2473 2010.8

Effect of Calcium Ion Concentrations on Osteogenic Differentiation and Hematopoietic Stem Cell Niche-Related Protein Expression in Osteoblasts Reviewed

Shinya Nakamura, Takuya Matsumoto, Jun-Ichi Sasaki, Hiroshi Egusa, Kuen Yong Lee, Takayoshi Nakano, Taiji Sohmura, Atsushi Nakahira

TISSUE ENGINEERING PART A 16 ( 8 ) 2467 - 2473 2010.8

Fabrication of Three-Dimensional Cell Constructs Using Temperature-Responsive Hydrogel Reviewed

Jun-Ichi Sasaki, Taka-Aki Asoh, Takuya Matsumoto, Hiroshi Egusa, Taiji Sohmura, Eben Alsberg, Mitsuru Akashi, Hirofumi Yatani

TISSUE ENGINEERING PART A 16 ( 8 ) 2497 - 2504 2010.8

Apatite Containing Aspartic Acid for Selective Protein Loading

M. Hafiz Uddin, T. Matsumoto, S. Ishihara, A. Nakahira, M. Okazaki, T. Sohmura

JOURNAL OF DENTAL RESEARCH 89 ( 5 ) 488 - 492 2010.5

Regulation of the protein-loading capacity of hydroxyapatite by mercaptosuccinic acid modification

Shiho Ishihara, Takuya Matsumoto, Takamasa Onoki, Mohammad Hafiz Uddin, Taiji Sohmura, Atsushi Nakahira

ACTA BIOMATERIALIA 6 ( 3 ) 830 - 835 2010.3

Synthesis of Hydroxyapatite Contining Manganese and Its Evaluation of Biocompatibility

FUJITANI Wataru, HAMADA Yoshinosuke, KAWAGUCHI Naomasa, MORI Seiji, DAITO Kiyoshi, UCHINAKA Ayako, MATSUMOTO Takuya, KOJIMA Yoshihisa, DAITO Michiharu, NAKANO Takayoshi, MATSUURA Nariaki

Nano Biomedicine 2 ( 1 ) 37 - 46 2010

Language：English Publisher：Nano Biomedical Society

Biomaterials made using hydroxyapatite (HAp) and calcium phosphates, which are inorganic components of hard tissues, have been developed to fill bone defects caused by various diseases. A biomaterial of HAp containing collagen, an organic component of bone, has also been developed. However, the physical and biological functions of these bone biomaterials remain inadequate.<br>Focusing on Zinc(Zn) and Manganese(Mn) ions out of the divalent cations involved in the activity of integrins, which are molecules existing in cell membranes, we synthesized three kinds of inorganic biomaterials—pure HAp, Zn-containing HAp (Zn-HAp), and Mn-containing HAp (Mn-HAp)—by a wet process. These three kinds of HAp powders were formed into pellets of 6mm diameter and 1mm thickness and their bio-adaptability was investigated.<br>In vitro experiments using osteoblast-like cells (MC3T3E1) demonstrated that Mn-HAp showed higher cell adhesion potential than pure HAp, indicating that Mn addition improves bioresponses. This may be because Mn ions eluted from Mn-HAp pellets activated integrins, which are closely related to the cell adhesion potential of MC3T3E1 cells to the surface of the pellets.

DOI： 10.11344/nano.2.37.

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In vitro engineering of transitional tissue by patterning and functional control of cells in fibrin gel Reviewed

Jun-Ichi Sasaki, Takuya Matsumoto, Hiroshi Egusa, Takayoshi Nakano, Takuya Ishimoto, Taiji Sohmura, Hirofumi Yatani

SOFT MATTER 6 ( 8 ) 1662 - 1667 2010

A small-molecule approach to bone regenerative medicine in dentistry

Hiroshi Egusa, Makio Saeki, Masanori Doi, Sho Fukuyasu, Takuya Matsumoto, Yoshinori Kamisaki, Hirofumi Yatani

Journal of Oral Biosciences 52 ( 2 ) 107 - 118 2010

Organic/inorganic composite material synthesized with osteoblastic cells Reviewed

T.Matsumoto, A.Mizuno, M.Okazaki, T.Nakano, T.Sohmura

2009.12

Mass Fabrication of Small Cell Spheroids by Using Micro-patterned Tissue Culture Plate

Akinari Iwasaki, Takuya Matsumoto, Go Tazaki, Hitoshi Tsuruta, Hiroshi Egusa, Hiroyuki Miyajima, Taiji Sohmura

ADVANCED ENGINEERING MATERIALS 11 ( 10 ) 801 - 804 2009.10

Enhanced bone regeneration via multimodal actions of synthetic peptide SVVYGLR on osteoprogenitors and osteoclasts

Hiroshi Egusa, Yoshitoshi Kaneda, Yoshihiro Akashi, Yoshinosuke Hamada, Takuya Matsumoto, Makio Saeki, Devang K. Thakor, Yasuhiko Tabata, Nariaki Matsuura, Hirofumi Yatani

BIOMATERIALS 30 ( 27 ) 4676 - 4686 2009.9

New concept bioceramics composed of octacalcium phosphate (OCP) and dicarboxylic acid-intercalated OCP via hydrothermal hot-pressing

Shiho Ishihara, Takuya Matsumoto, Takamasa Onoki, Taiji Sohmura, Atsushi Nakahira

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 29 ( 6 ) 1885 - 1888 2009.8

Clinical Evaluation of the Efficacy of Removing Microorganisms to Disinfect Patient-Derived Dental Impressions Reviewed

Hiroshi Egusa, Takao Watamoto, Takuya Matsumoto, Keiko Abe, Munemasa Kobayashi, Yoshihiro Akashi, Hirofumi Yatani

INTERNATIONAL JOURNAL OF PROSTHODONTICS 21 ( 6 ) 531 - 538 2008.11

Three-dimensional cell and tissue patterning in a strained fibrin gel system.

Matsumoto T, Sasaki J, Alsberg E, Egusa H, Yatani H, Sohmura T

PLoS ONE. 2 e1211 2008

Synthetic osteopontin-derived peptide SVVYGLR can induce neovascularization in artificial bone marrow scaffold bioniaterials Reviewed

Yoshinosuke Hamada, Hiroshi Egusa, Yoshitoshi Kaneda, Isao Hirata, Naomasa Kawaguchi, Takafumi Hirao, Takuya Matsumoto, Makiko Yao, Kiyoshi Daito, Mayuka Suzuki

DENTAL MATERIALS JOURNAL 26 ( 4 ) 487 - 492 2007.7

Synthetic osteopontin-derived peptide SVVYGLR can induce neovascularization in artificial bone marrow scaffold bioniaterials Reviewed

Yoshinosuke Hamada, Hiroshi Egusa, Yoshitoshi Kaneda, Isao Hirata, Naomasa Kawaguchi, Takafumi Hirao, Takuya Matsumoto, Makiko Yao, Kiyoshi Daito, Mayuka Suzuki

DENTAL MATERIALS JOURNAL 26 ( 4 ) 487 - 492 2007.7

Different behavior of implanted hydroxyapatite depending on morphology, size and crystallinity Reviewed

Takuya Matsumoto, Ken-ichi Tamine, Ryosuke Kagawa, Yoshinosuke Hamada, Masayuki Okazaki, Junzo Takahashi

JOURNAL OF THE CERAMIC SOCIETY OF JAPAN 114 ( 1333 ) 760 - 762 2006.9

Different behavior of implanted hydroxyapatite depending on morphology, size and crystallinity

Takuya Matsumoto, Ken-ichi Tamine, Ryosuke Kagawa, Yoshinosuke Hamada, Masayuki Okazaki, Junzo Takahashi

JOURNAL OF THE CERAMIC SOCIETY OF JAPAN 114 ( 1333 ) 760 - 762 2006.9

Role of acidic amino acid for regulating hydroxyapatite crystal growth Reviewed

Takuya Matsumoto, Masayuki Okazaki, Masahiro Inoue, Jun-Ichi Sasaki, Yoshinosuke Hamada, Junzo Takahashi

DENTAL MATERIALS JOURNAL 25 ( 2 ) 360 - 364 2006.6

Cell instructive polymers Reviewed

Takuya Matsumoto, David J. Mooney

TISSUE ENGINEERING I: SCAFFOLD SYSTEMS FOR TISSUE ENGINEERING 102 113 - 137 2006

Different behavior of implanted hydroxyapatite depending on morphology, size and crystallinity Reviewed

Takuya Matsumoto, Ken-Ichi Tamine, Ryosuke Kagawa, Yoshinosuke Hamada, Masayuki Okazaki, Junzo Takahashi

Journal of the Ceramic Society of Japan 114 ( 1333 ) 760 - 762 2006

Advantages of TOF-SIMS analysis of hydroxyapatite and fluorapatite in comparison with XRD, HR-TEM and FT-IR

M Okazaki, Hirata, I, T Matsumoto, J Takahashi

DENTAL MATERIALS JOURNAL 24 ( 4 ) 508 - 514 2005.12

Fabrication of Al2O3/BN nanocomposites by chemical processing and their mechanical properties

T Kusunose, YH Kim, T Sekino, T Matsumoto, N Tanaka, T Nakayama, K Niihara

JOURNAL OF MATERIALS RESEARCH 20 ( 1 ) 183 - 190 2005.1

Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo Reviewed

Y Hamada, K Yuki, M Okazaki, W Fujitani, T Matsumoto, MK Hashida, K Harutsugu, K Nokihara, M Daito, N Matsuura, J Takahashi

DENTAL MATERIALS JOURNAL 23 ( 4 ) 650 - 655 2004.12

Angiogenic activity of osteopontin-derived peptide SVVYGLR Reviewed

Y Hamada, K Nokihara, M Okazaki, W Fujitani, T Matsumoto, M Matsuo, Y Umakoshi, J Takahashi, N Matsuura

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 310 ( 1 ) 153 - 157 2003.10

Control of pore size in L-lactide/epsilon-caprolactone copolymer foams for tissue regeneration by the freeze-drying method Reviewed

H Nakao, SH Hyon, S Tsutsumi, T Matsumoto, J Takahashi

DENTAL MATERIALS JOURNAL 22 ( 3 ) 262 - 271 2003.9

Cytotoxic effect of methyl methacrylate on 4 cultured fibroblasts

M Taira, H Nakao, T Matsumoto, J Takahashi

INTERNATIONAL JOURNAL OF PROSTHODONTICS 13 ( 4 ) 311 - 315 2000.7

Inhibiting action of carbohydrates on the growth of fluorapatite crystals

T Matsumoto, M Okazaki, M Taira, J Takahashi

CARIES RESEARCH 34 ( 1 ) 26 - 32 2000.1

Organ-derived dependence of biomineralization in type I collagen gel Reviewed

T Matsumoto, M Okazaki, M Inoue, Y Hamada, M Taira, J Takahashi

BIOCERAMICS 192-1 709 - 712 2000

Functionally graded fluoridated apatites

M Okazaki, Y Miake, H Tohda, T Yanagisawa, T Matsumoto, J Takahashi

BIOMATERIALS 20 ( 15 ) 1421 - 1426 1999.8

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