2021/10/16 更新

写真a

モリタ ヒデトシ
森田 英利
MORITA Hidetoshi
所属
環境生命科学学域 教授
職名
教授
外部リンク

学位

  • 学術博士 ( 岡山大学 )

研究キーワード

  • 食品機能学

  • 乳酸菌ゲノム

  • 細菌ゲノム

  • 食品科学

  • プレバイオティクス

  • 応用微生物学

  • Food Science

  • Animal Science

  • Applied Microbiology

  • 微生物学

  • プロバイオティクス

  • ノトバイオートマウス

研究分野

  • ライフサイエンス / 応用微生物学

  • ライフサイエンス / 動物生産科学

  • ライフサイエンス / 動物生産科学

  • ライフサイエンス / 動物生理化学、生理学、行動学

  • ライフサイエンス / 実験動物学

  • ライフサイエンス / ゲノム生物学

  • ライフサイエンス / 食品科学

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学歴

  • 岡山大学   The Graduate School of Natural Science and Technology  

    1988年4月 - 1991年3月

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    国名: 日本国

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  • 岡山大学    

    1986年4月 - 1988年3月

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  • 岡山大学   Faculty of Agriculture  

    1982年4月 - 1986年3月

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    国名: 日本国

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経歴

  • 岡山大学大学院   環境生命科学研究科   教授

    2015年4月 - 現在

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  • 麻布大学   獣医学部 動物応用科学科   教授

    2010年10月 - 2015年3月

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  • 麻布大学 獣医学部 動物応用科学科 助教授   School of Veterinary Medicine, Department of Animal Science and Biotechnology

    2000年4月 - 2010年9月

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  • 麻布大学   獣医学部 動物応用科学科   講師

    1996年4月 - 2000年3月

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  • 麻布大学 獣医学部 環境畜産学科(現、動物応用科学科)助手   School of Veterinary Medicine

    1992年10月 - 1996年3月

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  • 米国ミネソタ州立大学 Food Microbiology研究室 博士研究員

    1991年5月 - 1992年9月

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所属学協会

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委員歴

  • 日本乳酸菌学会   評議員  

    2016年6月 - 現在   

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    団体区分:学協会

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  • 岡山県畜産協会   生乳検査運営委員(議長)  

    2015年11月 - 現在   

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    団体区分:学協会

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  • 日本畜産学会   常務理事・機関誌編集委員  

    2012年4月 - 2014年3月   

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    団体区分:学協会

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  • 内閣府消費者委員会 新開発食品調査部会、新開発食品評価第二調査会   委員  

    2010年1月 - 2015年3月   

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    団体区分:政府

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  • 腸内細菌学会   広報委員  

    2009年7月 - 現在   

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    団体区分:学協会

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  • 厚生労働省医薬品局食品案全部基準審査課管轄、薬事・食品衛生審議会食品衛生分科会新開発食品調査部会   審査委員  

    2009年1月 - 2010年1月   

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    団体区分:政府

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  • 日本NO学会   評議員  

    2000年5月 - 2016年5月   

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    団体区分:学協会

    日本NO学会

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  • 日本乳酸菌学会   庶務担当理事  

    1994年7月 - 2000年7月   

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    団体区分:学協会

    日本乳酸菌学会

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論文

  • Gut microorganisms act together to exacerbate inflammation in spinal cords 査読

    Eiji Miyauchi, Seok-Won Kim, Wataru Suda, Masami Kawasumi, Satoshi Onawa, Naoko Taguchi-Atarashi, Hidetoshi Morita, Todd D. Taylor, Masahira Hattori, Hiroshi Ohno

    Nature   585 ( 7823 )   102 - 106   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1038/s41586-020-2634-9

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    その他リンク: http://www.nature.com/articles/s41586-020-2634-9

  • Dysbiosis in the Salivary Microbiome Associated with IgA Nephropathy —A Japanese Cohort Study 査読

    Anushka Khasnobish, Lena Takayasu, Ken-ichi Watanabe, Tien Thi Thuy Nguyen, Kensuke Arakawa, Osamu Hotta, Kensuke Joh, Akiyo Nakano, Shuhei Hosomi, Masahira Hattori, Wataru Suda, Hidetoshi Morita

    Microbes Environ   36 ( 2 )   2021年5月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Selection of Immunobiotic Ligilactobacillus salivarius Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies 査読

    Binghui Zhou, Leonardo Albarracin, Yuhki Indo, Lorena Arce, Yuki Masumizu, Mikado Tomokiyo, Md. Aminul Islam, Valeria Garcia-Castillo, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Hidetoshi Morita, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa

    Microorganisms   8 ( 11 )   1659 - 1659   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different “immune” and “adhesion” phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.

    DOI: 10.3390/microorganisms8111659

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  • Collagen adhesion gene is associated with bloodstream infections caused by methicillin-resistant Staphylococcus aureus. 査読 国際誌

    Yasunori Iwata, Kenji Satou, Kengo Furuichi, Ikuko Yoneda, Takuhiro Matsumura, Masahiro Yutani, Yukako Fujinaga, Atsushi Hase, Hidetoshi Morita, Toshiko Ohta, Yasuko Senda, Yukiko Sakai-Takemori, Taizo Wada, Shinichi Fujita, Taito Miyake, Haruka Yasuda, Norihiko Sakai, Shinji Kitajima, Tadashi Toyama, Yasuyuki Shinozaki, Akihiro Sagara, Taro Miyagawa, Akinori Hara, Miho Shimizu, Yasutaka Kamikawa, Kazuho Ikeo, Shigeyuki Shichino, Satoshi Ueha, Takuya Nakajima, Kouji Matsushima, Shuichi Kaneko, Takashi Wada

    International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases   91   22 - 31   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES: Methicillin-resistant Staphylococcus aureus (MRSA) causes hospital- and community-acquired infections. It is not clear whether genetic characteristics of the bacteria contribute to disease pathogenesis in MRSA infection. We hypothesized that whole genome analysis of MRSA strains could reveal the key gene loci and/or the gene mutations that affect clinical manifestations of MRSA infection. METHODS: Whole genome sequences (WGS) of MRSA of 154 strains were analyzed with respect to clinical manifestations and data. Further, we evaluated the association between clinical manifestations in MRSA infection and genomic information. RESULTS: WGS revealed gene mutations that correlated with clinical manifestations of MRSA infection. Moreover, 12 mutations were selected as important mutations by Random Forest analysis. Cluster analysis revealed strains associated with a high frequency of bloodstream infection (BSI). Twenty seven out of 34 strains in this cluster caused BSI. These strains were all positive for collagen adhesion gene (cna) and have mutations in the locus, those were selected by Random Forest analysis. Univariate and multivariate analysis revealed that these gene mutations were the predictor for the incidence of BSI. Interestingly, mutant CNA protein showed lower attachment ability to collagen, suggesting that the mutant protein might contribute to the dissemination of bacteria. CONCLUSIONS: These findings suggest that the bacterial genotype affects the clinical characteristics of MRSA infection.

    DOI: 10.1016/j.ijid.2019.11.003

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  • Microbial colonization history modulates anxiety-like and complex social behavior in mice 査読

    Itsuka Kamimura, Ryou Kaneko, Hidetoshi Morita, Kazutaka Mogi, Takefumi Kikusui

    Neuroscience Research   S0168-0102 ( 19 )   30542 - 30545   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.neures.2020.01.001

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  • Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota 査読

    Takagaki R, Yoshizane C, Ishida Y, Sakurai T, Taniguchi Y, Watanabe H, Mitsuzumi H, Ushio S, Morita H

    Biosci Biotechnol Biochem   84 ( 4 )   824 - 831   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/09168451.2019.1704618

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  • Altered microbiota composition reflects enhanced communication in 15q11-13 CNV mice. 査読 国際誌

    Dian Eurike Septyaningtrias, Chia-Wen Lin, Rika Ouchida, Nobuhiro Nakai, Wataru Suda, Masahira Hattori, Hidetoshi Morita, Kenya Honda, Kota Tamada, Toru Takumi

    Neuroscience Research   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Autism spectrum disorder (ASD) is a complex and heterogeneous neurodevelopmental disorder. In addition to the core symptoms of ASD, many patients with ASD also show comorbid gut dysbiosis, which may lead to various gastrointestinal (GI) problems. Intriguingly, there is evidence that gut microbiota communicate with the central nervous system to modulate behavioral output through the gut-brain axis. To investigate how the microbiota composition is changed in ASD and to identify which microbes are involved in autistic behaviors, we performed a 16S rRNA gene-based metagenomics analysis in an ASD mouse model. Here, we focused on a model with human 15q11-13 duplication (15q dup), the most frequent chromosomal aberration or copy number variation found in ASD. Species diversity of the microbiome was significantly decreased in 15q dup mice. A combination of antibiotics treatment and behavioral analysis showed that neomycin improved social communication in 15q dup mice. Furthermore, comparison of the microbiota composition of mice treated with different antibiotics enabled us to identify beneficial operational taxonomic units (OTUs) for ultrasonic vocalization.

    DOI: 10.1016/j.neures.2019.12.010

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  • Gonadal steroid hormone secretion during the juvenile period depends on host-specific microbiota and contributes to the development of odor preference 査読

    Itsuka Kamimura, Akiyuki Watarai, Takuma Takamura, Atsushi Takeo, Kyoko Miura, Hidetoshi Morita, Kazutaka Mogi, Takefumi Kikusui

    Developmental Psychobiology   61 ( 5 )   670 - 678   2019年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1002/dev.21827

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  • Draft Genome Sequence of Butyricimonas faecihominis 30A1, Isolated from Feces of a Japanese Alzheimer's Disease Patient. 査読 国際誌

    Tien Thi Thuy Nguyen, Kenshiro Oshima, Hidehiro Toh, Anushka Khasnobish, Yusuke Fujii, Kensuke Arakawa, Hidetoshi Morita

    Microbiology Resource Announcements   8 ( 23 )   doi: 10.1128/MRA.00462-19   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Butyricimonas faecihominis 30A1, a butyrate-producing bacterium, was isolated from feces of a Japanese Alzheimer's disease patient. Here, we report the draft genome sequence of this organism. This paper is the first published report of the genomic sequence of a Butyricimonas sp.

    DOI: 10.1128/MRA.00462-19

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  • Draft Genome Sequence of Weissella viridescens UCO-SMC3, Isolated from the Slime of Helix aspersa Müller Snails 査読

    Apolinaria Garcia-Cancino, Leonardo Albarracin, Marcela Espinoza-Monje, Jorge Campos-Martin, Valeria Garcia-Castillo, Yuka Nakano, Wakako Ikeda-Ohtsubo, Cristian Guitierrez-Zamorano, Hidetoshi Morita, Haruki Kitazawa, Julio Villena

    Microbiology Resource Announcements   8 ( 11 )   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    This report describes the draft genome sequence of <named-content content-type="genus-species">Weissella viridescens</named-content> UCO-SMC3, isolated from <named-content content-type="genus-species">Helix aspersa</named-content> Müller slime. The reads were generated by a whole-genome sequencing (WGS) strategy on an Illumina MiSeq sequencer and were assembled into contigs with a total estimated size of 1,612,814 bp.

    DOI: 10.1128/mra.01654-18

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  • Oral faecal microbiota transplantation for the treatment of Clostridium difficile-associated diarrhoea in a dog: a case report 査読

    Sugita K, Yanuma N, Ohno H, Takahashi K, Kawano K, Morita H, Ohmori K

    BMC Veterinary Research   15 ( 1 )   11 - 14   2019年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1186/s12917-018-1754-z

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  • Fecal metabolite of a gnotobiotic mouse transplanted with gut microbiota from a patient with Alzheimer’s disease 査読

    Fujii Y, Nguyen TTT, Fujimura Y, Kameya N, Nakamura S, Arakawa K, Morita H

    Bioscience, Biotechnology, and Biochemistry   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/09168451.2019.1644149

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  • Berberine improved experimental chronic colitis by regulating interferon-γ- and IL-17A-productive lamina propria CD4+ T cells through AMPK activation 査読

    Takahara M, Takaki A, Hiraoka S, Adachi T, Shimomura Y, Matsushita H, Nguyen T, Koike K, Ikeda A, Takashima S, Yamasaki Y, Inokuchi T, Kinugasa H, Sugihara Y, Harada K, Eikawa S, Morita H, Udono H, Okada H

    Scientific Reports   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-019-48331-w

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  • Dysbiosis of the salivary microbiota in pediatric-onset primary sclerosing cholangitis and its potential as a biomarker 査読

    Kentaro Iwasawa, Wataru Suda, Tomoyuki Tsunoda, Manari Oikawa-Kawamoto, Shuichiro Umetsu, Lena Takayasu, Ayano Inui, Tomoo Fujisawa, Hidetoshi Morita, Tsuyoshi Sogo, Masahira Hattori

    Scientific Reports   8 ( 1 )   5480   2018年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nature Publishing Group  

    Primary sclerosing cholangitis (PSC) is a liver disease known for its frequent concurrence with inflammatory bowel disease. Dysbiosis of the gut microbiota in PSC was reported in several studies, but the microbiological features of the salivary microbiota in PSC have not been established. Here we compared the salivary microbial communities of 24 pediatric-onset PSC patients, 16 age-matched ulcerative colitis (UC) patients, and 24 healthy controls (HCs) by analyzing the bacterial 16S rRNA gene sequence data. The species-richness (α-diversity) showed no significant between-group differences, whereas the overall salivary microbiota structure (β-diversity) showed significant differences among the three groups. Taxonomic assignment revealed that the PSC salivary microbiota were characterized by significant decreases in the abundance of Rothia and Haemophilus compared to the HC group, and significantly decreased Haemophilus and increased Oribacterium compared to the UC group. By combining the genera selected by the random forest algorithm in machine learning, followed by confirmation with 10-fold cross-validation, we were able to distinguish the PSC group from the HC group with the area under the curve (AUC) of 0.7423, and from the UC group with the AUC of 0.8756. Our results indicate the potential of salivary microbiota as biomarkers for a noninvasive diagnosis of PSC.

    DOI: 10.1038/s41598-018-23870-w

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  • Gut microbiota-derived D-serine protects against acute kidney injury. 査読 国際誌

    Yusuke Nakade, Yasunori Iwata, Kengo Furuichi, Masashi Mita, Kenji Hamase, Ryuichi Konno, Taito Miyake, Norihiko Sakai, Shinji Kitajima, Tadashi Toyama, Yasuyuki Shinozaki, Akihiro Sagara, Taro Miyagawa, Akinori Hara, Miho Shimizu, Yasutaka Kamikawa, Kouichi Sato, Megumi Oshima, Shiori Yoneda-Nakagawa, Yuta Yamamura, Shuichi Kaneko, Tetsuya Miyamoto, Masumi Katane, Hiroshi Homma, Hidetoshi Morita, Wataru Suda, Masahira Hattori, Takashi Wada

    JCI insight   3 ( 20 )   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Gut microbiota-derived metabolites play important roles in health and disease. D-amino acids and their L-forms are metabolites of gut microbiota with distinct functions. In this study, we show the pathophysiologic role of D-amino acids in association with gut microbiota in humans and mice with acute kidney injury (AKI). In a mouse kidney ischemia/reperfusion model, the gut microbiota protected against tubular injury. AKI-induced gut dysbiosis contributed to the altered metabolism of D-amino acids. Among the D-amino acids, only D-serine was detectable in the kidney. In injured kidneys, the activity of D-amino acid oxidase was decreased. Conversely, the activity of serine racemase was increased. The oral administration of D-serine mitigated the kidney injury in B6 mice and D-serine-depleted mice. D-serine suppressed hypoxia-induced tubular damage and promoted posthypoxic tubular cell proliferation. Finally, the D-serine levels in circulation were significantly correlated with the decrease in kidney function in AKI patients. These results demonstrate the renoprotective effects of gut-derived D-serine in AKI, shed light on the interactions between the gut microbiota and the kidney in both health and AKI, and highlight D-serine as a potential new therapeutic target and biomarker for AKI.

    DOI: 10.1172/jci.insight.97957

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  • Cultivable butyrate-producing bacteria of elderly Japanese diagnosed with Alzheimer's disease. 査読

    Nguyen TTT, Fujimura Y, Mimura I, Fujii Y, Nguyen NL, Arakawa K, Morita H

    Journal of Microbiology   56 ( 10 )   760 - 771   2018年10月

  • Molecular mechanisms of Streptococcus pneumoniae-targeted autophagy via pneumolysin, Golgi-resident Rab41, and Nedd4-1-mediated K63-linked ubiquitination. 査読 国際誌

    Michinaga Ogawa, Ryuta Matsuda, Naoki Takada, Mikado Tomokiyo, Shouji Yamamoto, Sayaka Shizukusihi, Toshiyuki Yamaji, Yuko Yoshikawa, Mitsutaka Yoshida, Isei Tanida, Masato Koike, Miyo Murai, Hidetoshi Morita, Haruko Takeyama, Akihide Ryo, Jun-Lin Guan, Masahiro Yamamoto, Jun-Ichiro Inoue, Toru Yanagawa, Mitsunori Fukuda, Hiroshi Kawabe, Makoto Ohnishi

    Cellular microbiology   20 ( 8 )   e12846   2018年8月

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    記述言語:英語  

    Streptococcus pneumoniae is the most common causative agent of community-acquired pneumonia and can penetrate epithelial barriers to enter the bloodstream and brain. We investigated intracellular fates of S. pneumoniae and found that the pathogen is entrapped by selective autophagy in pneumolysin- and ubiquitin-p62-LC3 cargo-dependent manners. Importantly, following induction of autophagy, Rab41 was relocated from the Golgi apparatus to S. pneumoniae-containing autophagic vesicles (PcAV), which were only formed in the presence of Rab41-positive intact Golgi apparatuses. Moreover, subsequent localization and regulation of K48- and K63-linked polyubiquitin chains in and on PcAV were clearly distinguishable from each other. Finally, we found that E3 ligase Nedd4-1 was recruited to PcAV and played a pivotal role in K63-linked polyubiquitin chain (K63Ub) generation on PcAV, promotion of PcAV formation, and elimination of intracellular S. pneumoniae. These findings suggest that Nedd4-1-mediated K63Ub deposition on PcAV acts as a scaffold for PcAV biogenesis and efficient elimination of host cell-invaded pneumococci.

    DOI: 10.1111/cmi.12846

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  • Molecular mechanisms of Streptococcus pneumoniae-targeted autophagy via pneumolysin, Golgi-resident Rab41, and Nedd4-1 mediated K63-linked ubiquitination 査読

    Ogawa, Michinaga, Matsuda, Ryuta, Takada, Naoki, Tomokiyo, Mikado, Yamamoto, Shouji, Shizukusihi, Sayaka, Yamaji, Toshiyuki, Yoshikawa, Yuko, Yoshida, Mitsutaka, Tanida, Isei, Koike, Masato, Murai, Miyo, Morita, Hidetoshi, Takeyama, Haruko, Ryo, Akihide, Guan, Jun-Lin, Yamamoto, Masahiro, Inoue, Jun-Ichiro, Yanagawa, Toru, Fukuda, Mitsunori, Kawabe, Hiroshi, Ohnishi, Makoto

    Cellular microbiology   20 ( 8 )   2018年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Streptococcus pneumoniae is the most common causative agent of community-acquired pneumonia and can penetrate epithelial barriers to enter the bloodstream and brain. We investigated intracellular fates of S. pneumoniae and found that the pathogen is entrapped by selective autophagy in pneumolysin- and ubiquitin-p62-LC3 cargo-dependent manners. Importantly, following induction of autophagy, Rab41 was relocated from the Golgi apparatus to S. pneumoniae-containing autophagic vesicles (PcAV), which were only formed in the presence of Rab41-positive intact Golgi apparatuses. Moreover, subsequent localization and regulation of K48- and K63-linked polyubiquitin chains in and on PcAV were clearly distinguishable from each other. Finally, we found that E3 ligase Nedd4-1 was recruited to PcAV and played a pivotal role in K63-linked polyubiquitin chain (K63Ub) generation on PcAV, promotion of PcAV formation, and elimination of intracellular S. pneumoniae. These findings suggest that Nedd4-1-mediated K63Ub deposition on PcAV acts as a scaffold for PcAV biogenesis and efficient elimination of host cell-invaded pneumococci.

    DOI: 10.1111/cmi.12846

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  • Microbiota community structure in traditional fermented milk dadiah in Indonesia: Insights from high-throughput 16S rRNA gene sequencing 査読

    Sukma A, Toh H, Tien NTT, Fitria N, Mimura I, Kaneko R, Arakawa K, Morita H

    Milk Science International   71   1 - 3   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.25968/MSI.2018.1

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  • Towards standards for human fecal sample processing in metagenomic studies 査読

    Paul I. Costea, Georg Zeller, Shinichi Sunagawa, Eric Pelletier, Adriana Alberti, Florence Levenez, Melanie Tramontano, Marja Driessen, Rajna Hercog, Ferris-Elias Jung, Jens Roat Kultima, Matthew R. Hayward, Luis Pedro Coelho, Emma Allen-Vercoe, Laurie Bertrand, Michael Blaut, Jillian R. M. Brown, Thomas Carton, Stephanie Cools-Portier, Michelle Daigneault, Muriel Derrien, Anne Druesne, Willem M. de Vos, B. Brett Finlay, Harry J. Flint, Francisco Guarner, Masahira Hattori, Hans Heilig, Ruth Ann Luna, Johan van Hylckama Vlieg, Jana Junick, Ingeborg Klymiuk, Philippe Langella, Emmanuelle Le Chatelier, Volker Mai, Chaysavanh Manichanh, Jennifer C. Martin, Clementine Mery, Hidetoshi Morita, Paul W. O'Toole, Celine Orvain, Kiran Raosaheb Patil, John Penders, Soren Persson, Nicolas Pons, Milena Popova, Anne Salonen, Delphine Saulnier, Karen P. Scott, Bhagirath Singh, Kathleen Slezak, Patrick Veiga, James Versalovic, Liping Zhao, Erwin G. Zoetendal, S. Dusko Ehrlich, Joel Dore, Peer Bork

    NATURE BIOTECHNOLOGY   35 ( 11 )   1069 - +   2017年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Technical variation in metagenomic analysis must be minimized to confidently assess the contributions of microbiota to human health. Here we tested 21 representative DNA extraction protocols on the same fecal samples and quantified differences in observed microbial community composition. We compared them with differences due to library preparation and sample storage, which we contrasted with observed biological variation within the same specimen or within an individual over time. We found that DNA extraction had the largest effect on the outcome of metagenomic analysis. To rank DNA extraction protocols, we considered resulting DNA quantity and quality, and we ascertained biases in estimates of community diversity and the ratio between Gram-positive and Gram-negative bacteria. We recommend a standardized DNA extraction method for human fecal samples, for which transferability across labs was established and which was further benchmarked using a mock community of known composition. Its adoption will improve comparability of human gut microbiome studies and facilitate meta-analyses.

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  • Ectopic colonization of oral bacteria in the intestine drives T(H)1 cell induction and inflammation 査読

    Koji Atarashi, Wataru Suda, Chengwei Luo, Takaaki Kawaguchi, Iori Motoo, Seiko Narushima, Yuya Kiguchi, Keiko Yasuma, Eiichiro Watanabe, Takeshi Tanoue, Christoph A. Thaiss, Mayuko Sato, Kiminori Toyooka, Heba S. Said, Hirokazu Yamagami, Scott A. Rice, Dirk Gevers, Ryan C. Johnson, Julia A. Segre, Kong Chen, Jay K. Kolls, Eran Elinav, Hidetoshi Morita, Ramnik J. Xavier, Masahira Hattori, Kenya Honda

    SCIENCE   358 ( 6361 )   359 - +   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC ADVANCEMENT SCIENCE  

    Intestinal colonization by bacteria of oral origin has been correlated with several negative health outcomes, including inflammatory bowel disease. However, a causal role of oral bacteria ectopically colonizing the intestine remains unclear. Using gnotobiotic techniques, we show that strains of Klebsiella spp. isolated from the salivary microbiota are strong inducers of T helper 1 (T(H)1) cells when they colonize in the gut. These Klebsiella strains are resistant to multiple antibiotics, tend to colonize when the intestinal microbiota is dysbiotic, and elicit a severe gut inflammation in the context of a genetically susceptible host. Our findings suggest that the oral cavity may serve as a reservoir for potential intestinal pathobionts that can exacerbate intestinal disease.

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  • Profiling of faecal water and urine metabolites among Papua New Guinea highlanders believed to be adapted to low protein intake 査読

    Eriko Tomitsuka, Katsura Igai, Kiyoshi Tadokoro, Ayako Morita, Jun Baba, Wataru Suda, Andrew R. Greenhill, Paul F. Horwood, Kevin W. Soli, Peter M. Siba, Shingo Odani, Kazumi Natsuhara, Hidetoshi Morita, Masahiro Umezaki

    Metabolomics   13 ( 9 )   2017年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer New York LLC  

    Introduction: Adequate amount of proteins from foods are normally needed to maintain muscle mass of the human body. Although protein intakes of Papua New Guinea (PNG) highlanders are less than biologically adequate, protein deficiency related disorders have rarely been reported. It has been postulated that gut microbiota play a role in such low-protein-adaptation. Objective: To explore underlying biological mechanisms of low-protein adaptation among PNG highlanders by investigating metabolomic profiles of faecal water and urine. Methods: We performed metabolome analysis using faecal water extracted from faecal samples of PNG highlanders, PNG non-highlanders and Japanese subjects. We paid special attention to amino acids and other metabolites produced by gut microbiota, as well as to metabolites involved in nitrogen recycling in the human gut. Results: Our results indicated that amino acid levels were higher in faecal water from PNG highlanders than PNG non-highlanders, but amino acid levels did not differ between PNG highlanders and Japanese subjects. Among PNG highlander samples, amino acid levels tended to be higher in those who consumed less protein. Conclusion: We speculated that a greater proportion of urea was excreted to the intestine among the PNG highlanders than other groups, and that the urea was used for nitrogen salvage. Intestinal bacteria are essential for producing ammonia from urea and also for producing amino acids from ammonia, which is a key process in low-protein adaptation. Profiling the gut microbiota of PNG highlanders is an important avenue for further research into the mechanisms of low-protein adaptation.

    DOI: 10.1007/s11306-017-1243-6

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  • Clinical impact of pre-transplant gut microbial diversity on outcomes of allogeneic hematopoietic stem cell transplantation 査読

    Noriko Doki, Masahiro Suyama, Satoshi Sasajima, Junko Ota, Aiko Igarashi, Iyo Mimura, Hidetoshi Morita, Yuki Fujioka, Daisuke Sugiyama, Hiroyoshi Nishikawa, Yutaka Shimazu, Wataru Suda, Kozue Takeshita, Koji Atarashi, Masahira Hattori, Eiichi Sato, Kyoko Watakabe-Inamoto, Kosuke Yoshioka, Yuho Najima, Takeshi Kobayashi, Kazuhiko Kakihana, Naoto Takahashi, Hisashi Sakamaki, Kenya Honda, Kazuteru Ohashi

    ANNALS OF HEMATOLOGY   96 ( 9 )   1517 - 1523   2017年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Post-transplant microbial diversity in the gastrointestinal tract is closely associated with clinical outcomes following allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, little is known about the impact of the fecal microbiota before allo-HSCT. We analyzed fecal samples approximately 2 weeks before conditioning among 107 allo-HSCT recipients between 2013 and 2015. Microbial analysis was performed using 16S rRNA gene sequencing. Operational taxonomic unit-based microbial diversity was estimated by calculating the Shannon index. Patients were classified into three groups based on the diversity index: low (&lt;2), intermediate (2, 3), and high (&gt;3) diversity (18 (16.8%), 48 (44.9%), and 41 (38.3%) patients, respectively). There were no significant differences in the 20-month overall survival, cumulative incidence of relapse, and non-relapse mortality among three groups. The cumulative incidence of grade II to IV acute graft-versus-host disease (aGVHD) was similar among the three groups (low 55.6%; intermediate 35.4%; high 48.8%, p = 0.339, at day 100). Furthermore, we found no differences in the cumulative incidence of grade II to IV acute gastrointestinal GVHD among the three groups (low 38.9%; intermediate 21.3%; high 24.4%, p = 0.778, at day 100). Regarding the composition of microbiota before allo-HSCT, aGVHD patients showed a significantly higher abundance of phylum Firmicutes (p &lt; 0.01) and a lower tendency for Bacteroidetes (p = 0.106) than non-aGVHD patients. Maintenance of Bacteroidetes throughout allo-HSCT may be a strategy to prevent aGVHD.

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  • Characterisation of the faecal microbiota in Japanese patients with paediatric-onset primary sclerosing cholangitis 査読

    Kentaro Iwasawa, Wataru Suda, Tomoyuki Tsunoda, Manari Oikawa-Kawamoto, Shuichiro Umetsu, Ayano Inui, Tomoo Fujisawa, Hidetoshi Morita, Tsuyoshi Sogo, Masahira Hattori

    GUT   66 ( 7 )   1344 - +   2017年7月

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    記述言語:英語   出版者・発行元:BMJ PUBLISHING GROUP  

    DOI: 10.1136/gutjnl-2016-312533

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  • Faecal transplantation for the treatment of Clostridium difficile infection in a marmoset 査読

    Yumiko Yamazaki, Shinpei Kawarai, Hidetoshi Morita, Takefumi Kikusui, Atsushi Iriki

    BMC VETERINARY RESEARCH   13 ( 1 )   150   2017年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMED CENTRAL LTD  

    Background: The common marmoset has been used as an experimental animal for various purposes. Because its average weight ranges from 250 to 500 g, weight loss quickly becomes critical for sick animals. Therefore, effective and non-stressful treatment for chronic diseases, including diarrhoea, is essential.
    Case presentation: We report a case in which faecal microbiota transplantation (FMT) led to immediate recovery from chronic and recurrent diarrhoea caused by Clostridium difficile infection. A male common marmoset experienced chronic diarrhoea after antibiotic treatments. The animal experienced severe weight loss, and a faecal sample was confirmed to be C. difficile-positive but was negative for protozoa. Metronidazole was partially effective at the first administration but not after the recurrence of the clinical signs. Then, oral FMT was administered to the subject by feeding fresh faeces from healthy individuals mixed with the marmoset's usual food. We monitored the faeces by categorization into four groups: normal, loose, diarrhoea, and watery. After the first day of FMT treatment, the marmoset underwent a remarkable recovery from diarrhoea, and after the fourth day of treatment, a test for C. difficile was negative. The clinical signs did not recur. The marmoset recovered from sinusitis and bilateral dacryocystitis, which also did not recur, as a by-product of the improvement in its general health caused by the cessation of diarrhoea after the FMT.
    Conclusion: This is the first reported case of successful treatment of a marmoset using oral FMT. As seen in human patients, FMT was effective for the treatment of recurrent C. difficile infection in a captive marmoset.

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  • Confounding effects of microbiome on the susceptibility of TNFSF15 to Crohn's disease in the Ryukyu Islands 査読

    Shigeki Nakagome, Hiroshi Chinen, Atsushi Iraha, Akira Hokama, Yasuaki Takeyama, Shotaro Sakisaka, Toshiyuki Matsui, Judith R. Kidd, Kenneth K. Kidd, Heba S. Said, Wataru Suda, Hidetoshi Morita, Masahira Hattori, Tsunehiko Hanihara, Ryosuke Kimura, Hajime Ishida, Jiro Fujita, Fukunori Kinjo, Shuhei Mano, Hiroki Oota

    HUMAN GENETICS   136 ( 4 )   387 - 397   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Crohn's disease (CD) involves chronic inflammation in the gastrointestinal tract due to dysregulation of the host immune response to the gut microbiome. Even though the host-microbiome interactions are likely contributors to the development of CD, a few studies have detected genetic variants that change bacterial compositions and increase CD risk. We focus on one of the well-replicated susceptible genes, tumor necrosis factor superfamily member 15 (TNFSF15), and apply statistical analyses for personal profiles of genotypes and salivary microbiota collected from CD cases and controls in the Ryukyu Islands, southernmost islands of the Japanese archipelago. Our association test confirmed the susceptibility of TNFSF15 in the Ryukyu Islands. We found that the recessive model was supported to fit the observed genotype frequency of risk alleles slightly better than the additive model, defining the genetic effect on CD if a pair of the chromosomes in an individual consists of all risk alleles. The combined analysis of haplotypes and salivary microbiome from a small set of samples showed a significant association of the genetic effect with the increase of Prevotella, which led to a significant increase of CD risk. However, the genetic effect on CD disappeared if the abundance of Prevotella was low, suggesting the genetic contribution to CD is conditionally independent given a fixed amount of Prevotella. Although our statistical power is limited due to the small sample size, these results support an idea that the genetic susceptibility of TNFSF15 to CD may be confounded, in part, by the increase of Prevotella.

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  • Dysbiosis and compositional alterations with aging in the gut microbiota of patients with heart failure 査読

    Takehiro Kamo, Hiroshi Akazawa, Wataru Suda, Akiko Saga-Kamo, Yu Shimizu, Hiroki Yagi, Qing Liu, Seitaro Nomura, Atsuhiko T. Naito, Norifumi Takeda, Mutsuo Harada, Haruhiro Toko, Hidetoshi Kumagai, Yuichi Ikeda, Eiki Takimoto, Jun-ichi Suzuki, Kenya Honda, Hidetoshi Morita, Masahira Hattori, Issei Komuro

    PLOS ONE   12 ( 3 )   e0174099   2017年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Emerging evidence has suggested a potential impact of gut microbiota on the pathophysiology of heart failure (HF). However, it is still unknown whether HF is associated with dysbiosis in gut microbiota. We investigated the composition of gut microbiota in patients with HF to elucidate whether gut microbial dysbiosis is associated with HF. We performed 16S ribosomal RNA gene sequencing of fecal samples obtained from 12 HF patients and 12 age-matched healthy control (HC) subjects, and analyzed the differences in gut microbiota. We further compared the composition of gut microbiota of 12 HF patients younger than 60 years of age with that of 10 HF patients 60 years of age or older. The composition of gut microbial communities of HF patients was distinct from that of HC subjects in both unweighted and weighted UniFrac analyses. Eubacterium rectale and Dorea longicatena were less abundant in the gut microbiota of HF patients than in that of HC subjects. Compared to younger HF patients, older HF patients had diminished proportions of Bacteroidetes and larger quantities of Proteobacteria. The genus Faecalibacterium was depleted, while Lactobacillus was enriched in the gut microbiota of older HF patients. These results suggest that patients with HF harbor significantly altered gut microbiota, which varies further according to age. New concept of heart-gut axis has a great potential for breakthroughs in the development of novel diagnostic and therapeutic approach for HF.

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  • Draft Genome Sequence of Bifidobacterium lemurum DSM 28807T Isolated from the Gastrointestinal Tracts of Ring-Tailed Lemurs (Lemur catta) 査読

    Hidehiro Toh, Takehiro Matsubara, Shuta Tomida, Iyo Mimura, Kensuke Arakawa, Takefumi Kikusui, Hidetoshi Morita

    Genome Announcements   5 ( 8 )   2017年2月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <title>ABSTRACT</title>

    <named-content content-type="genus-species">Bifidobacterium lemurum</named-content> DSM 28807<sup>T</sup> was isolated from the gastrointestinal tracts of ring-tailed lemurs (<italic>Lemur catta</italic>). Here, we report the first draft genome sequence of this organism.

    DOI: 10.1128/genomea.01656-16

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  • Draft Genome Sequence of Probiotic Lactobacillus acidophilus Strain L-55 Isolated from a Healthy Human Gut 査読

    Yusuke Fujii, Hidehiro Toh, Takehiro Matsubara, Shuta Tomida, Co Thi Kim Nguyen, Iyo Mimura, Shoji Nakamura, Hidetoshi Morita

    Genome Announcements   4 ( 6 )   2016年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    Probiotic
    <italic>Lactobacillus acidophilus</italic>
    L-55 was isolated from a healthy human gut. Here, we report the draft genome sequence of this organism.

    DOI: 10.1128/genomea.01357-16

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  • Fecal microbiota transplantation for patients with steroid-resistant acute graft-versus-host disease of the gut 査読

    Kazuhiko Kakihana, Yuki Fujioka, Wataru Suda, Yuho Najima, Go Kuwata, Satoshi Sasajima, Iyo Mimura, Hidetoshi Morita, Daisuke Sugiyama, Hiroyoshi Nishikawa, Masahira Hattori, Yutaro Hino, Shuntaro Ikegawa, Keita Yamamoto, Takashi Toya, Noriko Doki, Koichi Koizumi, Kenya Honda, Kazuteru Ohashi

    BLOOD   128 ( 16 )   2083 - 2088   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC HEMATOLOGY  

    Increasing evidence indicates that the gut microbiota is closely associated with acute graft-versus-host disease (aGVHD) in stem cell transplantation (SCT). Fecal microbiota transplantation (FMT) could represent an alternative treatment option for aGVHD. However, FMT for SCT patients carries a potential risk of infection by infused microbiota because of the severely immunosuppressed status. We therefore conducted a pilot study to evaluate the safety of FMT in SCT. A total of 4 patients with steroid-resistant (n = 3) or steroid-dependent gut aGVHD (n = 1) received FMT. No severe adverse events attributed to FMT were observed. All patients responded to FMT, with 3 complete responses and 1 partial response. Temporal dynamics of microbiota seemed to be linked to the gut condition of patients and peripheral effector regulatory T cells also increased during response to FMT. FMT was safely performed in our patients and might offer a novel therapeutic option for aGVHD. This trial was registered at the University Hospital Medical Information Network (https://upload.umin.ac.jp/cgi-open-bin/icdr_e/ctr_view.cgi?recptno=R000017575) as #UMIN000015115.

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  • Ecophysiological consequences of alcoholism on human gut microbiota: implications for ethanol-related pathogenesis of colon cancer 査読

    Atsuki Tsuruya, Akika Kuwahara, Yuta Saito, Haruhiko Yamaguchi, Takahisa Tsubo, Shogo Suga, Makoto Inai, Yuichi Aoki, Seiji Takahashi, Eri Tsutsumi, Yoshihide Suwa, Hidetoshi Morita, Kenji Kinoshita, Yukari Totsuka, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Takeshi Mizukami, Akira Yokoyama, Takefumi Shimoyama, Toru Nakayama

    SCIENTIFIC REPORTS   6   27923   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Chronic consumption of excess ethanol increases the risk of colorectal cancer. The pathogenesis of ethanol-related colorectal cancer (ER-CRC) is thought to be partly mediated by gut microbes. Specifically, bacteria in the colon and rectum convert ethanol to acetaldehyde (AcH), which is carcinogenic. However, the effects of chronic ethanol consumption on the human gut microbiome are poorly understood, and the role of gut microbes in the proposed AcH-mediated pathogenesis of ER-CRC remains to be elaborated. Here we analyse and compare the gut microbiota structures of non-alcoholics and alcoholics. The gut microbiotas of alcoholics were diminished in dominant obligate anaerobes (e.g., Bacteroides and Ruminococcus) and enriched in Streptococcus and other minor species. This alteration might be exacerbated by habitual smoking. These observations could at least partly be explained by the susceptibility of obligate anaerobes to reactive oxygen species, which are increased by chronic exposure of the gut mucosa to ethanol. The AcH productivity from ethanol was much lower in the faeces of alcoholic patients than in faeces of non-alcoholic subjects. The faecal phenotype of the alcoholics could be rationalised based on their gut microbiota structures and the ability of gut bacteria to accumulate AcH from ethanol.

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  • Two FOXP3(+)CD4(+) T cell subpopulations distinctly control the prognosis of colorectal cancers 査読

    Takuro Saito, Hiroyoshi Nishikawa, Hisashi Wada, Yuji Nagano, Daisuke Sugiyama, Koji Atarashi, Yuka Maeda, Masahide Hamaguchi, Naganari Ohkura, Eiichi Sato, Hirotsugu Nagase, Junichi Nishimura, Hirofumi Yamamoto, Shuji Takiguchi, Takeshi Tanoue, Wataru Suda, Hidetoshi Morita, Masahira Hattori, Kenya Honda, Masaki Mori, Yuichiro Doki, Shimon Sakaguchi

    NATURE MEDICINE   22 ( 6 )   679 - +   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    CD4(+) T cells that express the forkhead box P3 (FOXP3) transcription factor function as regulatory T (T-reg) cells and hinder effective immune responses against cancer cells(1-3). Abundant Treg cell infiltration into tumors is associated with poor clinical outcomes in various types of cancers(3-7). However, the role of T-reg cells is controversial in colorectal cancers (CRCs), in which FOXP3(+) T cell infiltration indicated better prognosis in some studies(6-9). Here we show that CRCs, which are commonly infiltrated by suppression-competent FOXP3(hi) T-reg cells, can be classified into two types by the degree of additional infiltration of FOXP3(lo) nonsuppressive T cells(10). The latter, which are distinguished from FOXP3(+) T-reg cells by non-expression of the naive T cell marker CD45RA and instability of FOXP3, secreted inflammatory cytokines. Indeed, CRCs with abundant infiltration of FOXP3(lo) T cells showed significantly better prognosis than those with predominantly FOXP3(hi) T-reg cell infiltration. Development of such inflammatory FOXP3(lo) non-T-reg cells may depend on secretion of interleukin (IL)-12 and transforming growth factor (TGF)-beta by tissues and their presence was correlated with tumor invasion by intestinal bacteria, especially Fusobacterium nucleatum. Thus, functionally distinct subpopulations of tumor-infiltrating FOXP3(+) T cells contribute in opposing ways to determining CRC prognosis. Depletion of FOXP3(hi) T-reg cells from tumor tissues, which would augment antitumor immunity, could thus be used as an effective treatment strategy for CRCs and other cancers, whereas strategies that locally increase the population of FOXP3(lo) non-T-reg cells could be used to suppress or prevent tumor formation.

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  • Draft Genome Sequence of Leuconostoc mesenteroides 213M0, Isolated from Traditional Fermented Mare Milk Airag in Bulgan Aimag, Mongolia 査読

    Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Chihiro Hano, Saki Yoshida, Tsognemekh Bolormaa, Sedkhuu Burenjargal, Co Thi Kim Nguyen, Kosuke Tashiro, Kensuke Arakawa, Taku Miyamoto

    Genome Announcements   4 ( 2 )   2016年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <italic>Leuconostoc mesenteroides</italic>
    213M0 was isolated from traditional fermented mare milk airag in Bulgan Aimag, Mongolia. This strain produces a listericidal bacteriocin-like inhibitory substance. Here, we report the draft genome sequence of this organism.

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  • Draft Genome Sequence of Leuconostoc mesenteroides 406 Isolated from the Traditional Fermented Mare Milk Airag in Tuv Aimag, Mongolia 査読

    Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Chihiro Hano, Saki Yoshida, Tien Thi Thuy Nguyen, Wulijideligen, Kosuke Tashiro, Kensuke Arakawa, Taku Miyamoto

    Genome Announcements   4 ( 2 )   2016年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <italic>Leuconostoc mesenteroides</italic>
    406 was isolated from the traditional fermented mare milk airag in Tuv Aimag, Mongolia. This strain produces an antilisterial bacteriocin. Here, we report the draft genome sequence of this organism.

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  • The gut microbiome of healthy Japanese and its microbial and functional uniqueness 査読

    Suguru Nishijima, Wataru Suda, Kenshiro Oshima, Seok-Won Kim, Yuu Hirose, Hidetoshi Morita, Masahira Hattori

    DNA RESEARCH   23 ( 2 )   125 - 133   2016年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The human gut microbiome has profound influences on the host's health largely through its interference with various intestinal functions. As recent studies have suggested diversity in the human gut microbiome among human populations, it will be interesting to analyse how gut microbiome is correlated with geographical, cultural, and traditional differences. The Japanese people are known to have several characteristic features such as eating a variety of traditional foods and exhibiting a low BMI and long life span. In this study, we analysed gut microbiomes of the Japanese by comparing the metagenomic data obtained from 106 Japanese individuals with those from 11 other nations. We found that the composition of the Japanese gut microbiome showed more abundant in the phylum Actinobacteria, in particular in the genus Bifidobacterium, than other nations. Regarding the microbial functions, those of carbohydrate metabolism were overrepresented with a concurrent decrease in those for replication and repair, and cell motility. The remarkable low prevalence of genes for methanogenesis with a significant depletion of the archaeon Methanobrevibacter smithii and enrichment of acetogenesis genes in the Japanese gut microbiome compared with others suggested a difference in the hydrogen metabolism pathway in the gut between them. It thus seems that the gut microbiome of the Japanese is considerably different from those of other populations, which cannot be simply explained by diet alone. We postulate possible existence of hitherto unknown factors contributing to the population-level diversity in human gut microbiomes.

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  • Draft Genome Sequence of Coccoid Lactobacillus equigenerosi NRIC 0697 T Isolated from the Gastrointestinal Tracts of Healthy Thoroughbreds 査読

    Hidehiro Toh, Akiyo Nakano, Co Thi Kim Nguyen, Iyo Mimura, Kensuke Arakawa, Kosuke Tashiro, Takefumi Kikusui, Hidetoshi Morita

    Genome Announcements   4 ( 1 )   2016年2月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <italic>Lactobacillus equigenerosi</italic>
    NRIC 0697
    <sup>T</sup>
    was isolated from the gastrointestinal tracts of healthy thoroughbreds. This strain produced unique spherical or oval cells, which is rare in the genus
    <italic>Lactobacillus</italic>
    . Here, we report the draft genome sequence of this strain.

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  • Draft Genome Sequence of Streptococcus orisasini SH06, Isolated from a Healthy Thoroughbred Gastrointestinal Tract 査読

    Misako Takagi, Akiyo Nakano, Hidehiro Toh, Kenshiro Oshima, Kensuke Arakawa, Fumihiko Nakajima, Kosuke Tashiro, Tekefumi Kikusui, Fujitoshi Yanagida, Hidetoshi Morita

    Genome Announcements   4 ( 1 )   2016年2月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <italic>Streptococcus orisasini</italic>
    SH06 was isolated from a healthy thoroughbred gastrointestinal tract. Here, we report the draft genome sequence of this organism. This paper is the first published report of the genomic sequence of
    <italic>S. orisasini</italic>
    .

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  • Draft Genome Sequence of Bifidobacterium aesculapii DSM 26737 T , Isolated from Feces of Baby Common Marmoset 査読

    Hidehiro Toh, Yumiko Yamazaki, Kosuke Tashiro, Shinpei Kawarai, Kenshiro Oshima, Akiyo Nakano, Co Nguyen Thi Kim, Iyo Mimura, Kensuke Arakawa, Atsushi Iriki, Takefumi Kikusui, Hidetoshi Morita

    Genome Announcements   3 ( 6 )   2015年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <italic>Bifidobacterium aesculapii</italic>
    DSM 26737
    <sup>T</sup>
    was isolated from feces of baby common marmoset. Here, we report the draft genome sequence of this organism. This paper is the first published report of the genomic sequence of
    <italic>B. aesculapii</italic>
    .

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  • Bacillus hisashii sp nov., isolated from the caeca of gnotobiotic mice fed with thermophile-fermented compost 査読

    Ayaka Nishida, Hirokuni Miyamoto, Sankichi Horiuchi, Ryo Watanabe, Hidetoshi Morita, Shinji Fukuda, Hiroshi Ohno, Shizuko Ichinose, Hisashi Miyamoto, Hiroaki Kodama

    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY   65 ( 11 )   3944 - 3949   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    A taxonomic study was performed on 15 bacterial isolates from the caeca of gnotobiotic mice that had been fed with thermophile-fermented compost. The 15 isolates were thermophilic, Gram-stain-positive, facultatively anaerobic, endospore-forming bacteria, and were most closely related to Bacillus thermoamylovorans CNCM I-1378(T). The 16S rRNA gene sequence of strain N-11(T), selected as representative of this new group, showed a similarity of 99.4 % with Bacillus thermoamylovorans CNCM I-1378(T), 94.7 % with Bacillus thermolactis R-6488(T), and 94.4 % with Bacillus kokeshiiformis MO-04(T). The isolates were then classified into two distinct groups based on a (GTG) 5-fingerprint analysis. Two isolates, N-11(T) and N-21, were the representatives of these two groups, respectively. The N-11(T) and N-21 isolates showed 66-71 % DNA-DNA relatedness with one other, but had less than 37 % DNA-DNA relatedness with B. thermoamylovorans LMG 18084(T). The other 13 isolates showed DNA-DNA relatedness values above 74 % with the N-11(T) isolate. All 15 isolates grew at 25-60 degrees C (optimum 50 degrees C), pH 6-8 (optimum pH 7) and were capable of growing on a medium containing 6 % (w/v) NaCl (optimum 0.5 %). The 15 isolates could be distinguished from B. thermoamylovorans LMG 18084(T) because they showed Tween 80 hydrolysis activity and did not produce acid from melibiose. The major fatty acids were anteiso-C-15 : 0, C-16 : 0, iso-C-15 : 0, iso-C-14 : 0 and iso-C-16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and several unidentified phospholipids. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The menaquinone was MK-7. The DNA G + C content was 37.9 mol%. Based on the phenotypic properties, the 15 strains represent a novel species of the genus Bacillus, for which the name Bacillus hisashii sp. nov. is proposed. The type strain is N-11(T) (=NRBC 110226(T) =LMG 28201(T)).

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  • Complete genome sequence of Lactobacillus acetotolerans RIB 9124 (NBRC 13120) isolated from putrefied (hiochi) Japanese sake 査読

    Hidehiro Toh, Hidetoshi Morita, Hiroyuki Tsuji, Kazuhiro Iwashita, Nami Goto, Jiro Nakayama, Mitsuo Sekine, Yumiko Kato, Ken-ichiro Suzuki, Nobuyuki Fujita

    JOURNAL OF BIOTECHNOLOGY   214   214 - 215   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Lactobacillus acetotolerans RIB 9124 (NBRC 13120) was isolated from putrefied (hiochi) Japanese sake. Here we report the complete genome sequence of this organism. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a L. acetotolerans strain. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jbiotec.2015.09.006

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  • Complete genome sequence of Bifidobacterium angulatum JCM 7096(T) isolated from human feces 査読

    Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Misa Kiuchi, Hiromi Kuroyanagi, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

    JOURNAL OF BIOTECHNOLOGY   211   10 - 11   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Bifidobacterium angulatum JCM 7096T was isolated from human feces. Here we report the complete genome sequence of this organism. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. angulatum strain. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jbiotec.2015.06.412

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  • Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells 査読

    Koji Atarashi, Takeshi Tanoue, Minoru Ando, Nobuhiko Kamada, Yuji Nagano, Seiko Narushima, Wataru Suda, Akemi Imaoka, Hiromi Setoyama, Takashi Nagamori, Eiji Ishikawa, Tatsuichiro Shima, Taeko Hara, Shoichi Kado, Toshi Jinnohara, Hiroshi Ohno, Takashi Kondo, Kiminori Toyooka, Eiichiro Watanabe, Shin-ichiro Yokoyama, Shunji Tokoro, Hiroshi Mori, Yurika Noguchi, Hidetoshi Morita, Ivaylo I. Ivanov, Tsuyoshi Sugiyama, Gabriel Nunez, J. Gray Camp, Masahira Hattori, Yoshinori Umesaki, Kenya Honda

    CELL   163 ( 2 )   367 - 380   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CELL PRESS  

    Intestinal Th17 cells are induced and accumulate in response to colonization with a subgroup of intestinal microbes such as segmented filamentous bacteria (SFB) and certain extracellular pathogens. Here, we show that adhesion of microbes to intestinal epithelial cells (ECs) is a critical cue for Th17 induction. Upon monocolonization of germ-free mice or rats with SFB indigenous to mice (M-SFB) or rats (R-SFB), M-SFB and R-SFB showed host-specific adhesion to small intestinal ECs, accompanied by host-specific induction of Th17 cells. Citrobacter rodentium and Escherichia coli O157 triggered similar Th17 responses, whereas adhesion-defective mutants of these microbes failed to do so. Moreover, a mixture of 20 bacterial strains, which were selected and isolated from fecal samples of a patient with ulcerative colitis on the basis of their ability to cause a robust induction of Th17 cells in the mouse colon, also exhibited EC-adhesive characteristics.

    DOI: 10.1016/j.cell.2015.08.058

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  • Complete genome sequence of Bifidobacterium bifidum JCM 1255(T) isolated from feces of a breast-fed infant 査読

    Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Chie Shindo, Keiko Komiya, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

    JOURNAL OF BIOTECHNOLOGY   210   66 - 67   2015年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Bifidobacterium bifidum JCM 1255(T) was isolated from feces of a breast-fed infant. Here we report the complete genome sequence of this organism. (C) 2015 Elsevier B.V. All rights reserved.

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  • Complete genome sequence of Bifidobacterium breve JCM 1192(T) isolated from infant feces 査読

    Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Emi Omori, Yasue Hattori, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

    JOURNAL OF BIOTECHNOLOGY   210   81 - 82   2015年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Bifidobacterium breve JCM 1192(T) was isolated from infant feces. Here, we report the complete genome sequence of this organism. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jbiotec.2015.06.414

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  • Complete genome sequence of Bifidobacterium catenulatum JCM 1194(T) isolated from human feces 査読

    Hidetoshi Moritaa, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Naoko Yamashita, Erica Iioka, Kensuke Arakawa, Wataru Suda, Kenya Honda, Masahira Hattori

    JOURNAL OF BIOTECHNOLOGY   210   25 - 26   2015年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Bifidobacterium catenulatum JCM 1194(T) was isolated from human feces. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. catenulatum strain. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jbiotec.2015.06.415

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  • Complete genome sequence of Bifidobacterium pseudocatenulatum JCM 1200(T) isolated from infant feces 査読

    Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Kensuke Arakawa, Yukiko Takayama, Rina Kurokawa, Kageyasu Takanashi, Kenya Honda, Masahira Hattori

    JOURNAL OF BIOTECHNOLOGY   210   68 - 69   2015年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Bifidobacterium pseudocatenulatum JCM 1200(T) was isolated from infant feces. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. pseudocatenulatum strain. (C) 2015 Elsevier B.V. All rights reserved.

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  • Complete Genome Sequence of Scardovia inopinata JCM 12537 T , Isolated from Human Dental Caries 査読

    Kenshiro Oshima, Jun-ichiro Hayashi, Hidehiro Toh, Akiyo Nakano, Emi Omori, Yasue Hattori, Hidetoshi Morita, Kenya Honda, Masahira Hattori

    Genome Announcements   3 ( 3 )   2015年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    DOI: 10.1128/genomea.00481-15

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  • Complete Genome Sequence of Parascardovia denticolens JCM 12538 T , Isolated from Human Dental Caries 査読

    Kenshiro Oshima, Jun-ichiro Hayashi, Hidehiro Toh, Akiyo Nakano, Chie Shindo, Keiko Komiya, Hidetoshi Morita, Kenya Honda, Masahira Hattori

    Genome Announcements   3 ( 3 )   2015年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    DOI: 10.1128/genomea.00485-15

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  • Influence of Proton-Pump Inhibitors on the Luminal Microbiota in the Gastrointestinal Tract 査読

    Ayumi Tsuda, Wataru Suda, Hidetoshi Morita, Kageyasu Takanashi, Atsushi Takagi, Yasuhiro Koga, Masahira Hattori

    CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY   6   e89   2015年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    OBJECTIVES: The objective of this study was to investigate comparatively the influence of proton-pump inhibitors (PPI) administration on three bacterial communities in the oral cavity, stomach, and colon along the alimentary tract.
    METHODS: Forty-five subjects including 18 patients taking PPI were enrolled. Stimulated saliva, gastric fluid (GF), and feces were obtained from each subject for the microbiota analysis through bacterial 16S rRNA gene profiling using the pyrosequencing method.
    RESULTS: The species richness (alpha diversity) was similar among these three microbiota, whereas the interindividual diversity (beta diversity) was much higher in the fecal microbiota compared with that in the others. The UniFrac analysis indicated that the salivary and GF microbiota were similar to one another; however, both differed greatly from the fecal microbiota in the overall bacterial community structure. In the comparison between PPI-users and PPI-nonusers, a bacterial cell number increase of similar to 1,000 times was found in the GF of PPI-users using culturing methods, whereas the bacterial number and composition were nearly identical between the two groups using quantitative PCR and a similarity search based on 16S profiling. The beta diversity significantly increased in both the salivary and GF microbiota of PPI-users compared with PPI-nonusers.
    CONCLUSIONS: These results suggest that the GF microbiota has recently moved from the saliva. Bacterial overgrowth in the GF by PPI administration may be due to a lack of killing rather than proliferation of the bacteria in the acid-suppressed stomach. The biological significance of the increase in beta diversity by PPI administration remains unclear.

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  • Protective effect of aqueous extracts from Rhizopus oryzae on liver injury induced by carbon tetrachloride in rats 査読

    Takehito Suzuki, Hideo Fukuoka, Setsuo Ushikoshi, Reiichiro Sato, Hidetoshi Morita, Tatsuya Takizawa

    ANIMAL SCIENCE JOURNAL   86 ( 5 )   532 - 540   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Hepatoprotective effects of Rhizopusoryzae/U-1 aqueous extract (RU) were demonstrated in carbon tetrachloride (CCl4)-induced liver-injured rats. In order to investigate the RU effects, the rats were administered RU at a dose of 10 or 100mg/kg of body weight for 10 days before induction of the liver injury by oral administration of CCl4 (125mg/kg body weight). (i) Pretreatment with RU caused a significant decrease in serum lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities that were increased by the administration of CCl4. (ii) RU pretreatment (100mg/kg) increased 5-bromo-2-deoxyuridine incorporation at 48h after CCl4 treatment in hepatocytes. (iii) Histological hematoxylin and eosin staining of the liver showed that RU pretreatment reduced the damage induced by CCl4 administration. (iv) Reverse transcriptase PCR analysis showed RU retreatment caused a transient but significant increase in hepatocyte growth factor (HGF) and a sustained and significant increase in insulin-like growth factor-I (IGF-I) gene expression in hepatocytes injured by CCl4 treatment. From these results, we conclude that oral pre-administration of RU was effective to suppress liver injury induced by the subsequent oral CCl4 administration, and RU-induced increase in IGF-I and HGF gene expression may be, even in part, involved in biological actions of RU in rats.

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  • Complete Genome Sequence of Gardnerella vaginalis Strain JCM 11026 T , Isolated from Vaginal Tracts of Women 査読

    Kenshiro Oshima, Shin Hisamatsu, Hidehiro Toh, Akiyo Nakano, Misa Kiuchi, Hiromi Kuroyanagi, Hidetoshi Morita, Masahira Hattori

    Genome Announcements   3 ( 2 )   2015年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    DOI: 10.1128/genomea.00286-15

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  • Complete Genome Sequence of Bifidobacterium scardovii Strain JCM 12489 T , Isolated from Human Blood 査読

    Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Naoko Yamashita, Erica Iioka, Rina Kurokawa, Hidetoshi Morita, Masahira Hattori

    Genome Announcements   3 ( 2 )   2015年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    DOI: 10.1128/genomea.00285-15

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  • Complete Genome Sequence of Bifidobacterium dentium Strain JCM 1195 T , Isolated from Human Dental Caries 査読

    Hidehiro Toh, Jun-ichiro Hayashi, Kenshiro Oshima, Akiko Nakano, Yukiko Takayama, Kageyasu Takanashi, Hidetoshi Morita, Masahira Hattori

    Genome Announcements   3 ( 2 )   2015年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    DOI: 10.1128/genomea.00284-15

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  • Complete Genome Sequence of Bifidobacterium kashiwanohense JCM 15439 T , Isolated from Feces from a Healthy Japanese Infant 査読

    Hidetoshi Morita, Hidehiro Toh, Akiyo Nakano, Kenshiro Oshima, Misako Takagi, Wataru Suda, Soichi Tanabe, Masahira Hattori

    Genome Announcements   3 ( 2 )   2015年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    DOI: 10.1128/genomea.00255-15

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  • Dysbiosis in the Gut Microbiota of Patients with Multiple Sclerosis, with a Striking Depletion of Species Belonging to Clostridia XIVa and IV Clusters. 査読 国際誌

    Sachiko Miyake, Sangwan Kim, Wataru Suda, Kenshiro Oshima, Masakazu Nakamura, Takako Matsuoka, Norio Chihara, Atsuko Tomita, Wakiro Sato, Seok-Won Kim, Hidetoshi Morita, Masahira Hattori, Takashi Yamamura

    PloS one   10 ( 9 )   e0137429   2015年

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    記述言語:英語  

    The pathogenesis of multiple sclerosis (MS), an autoimmune disease affecting the brain and spinal cord, remains poorly understood. Patients with MS typically present with recurrent episodes of neurological dysfunctions such as blindness, paresis, and sensory disturbances. Studies on experimental autoimmune encephalomyelitis (EAE) animal models have led to a number of testable hypotheses including a hypothetical role of altered gut microbiota in the development of MS. To investigate whether gut microbiota in patients with MS is altered, we compared the gut microbiota of 20 Japanese patients with relapsing-remitting (RR) MS (MS20) with that of 40 healthy Japanese subjects (HC40) and an additional 18 healthy subjects (HC18). All the HC18 subjects repeatedly provided fecal samples over the course of months (158 samples in total). Analysis of the bacterial 16S ribosomal RNA (rRNA) gene by using a high-throughput culture-independent pyrosequencing method provided evidence of a moderate dysbiosis in the structure of gut microbiota in patients with MS. Furthermore, we found 21 species that showed significant differences in relative abundance between the MS20 and HC40 samples. On comparing MS samples to the 158 longitudinal HC18 samples, the differences were found to be reproducibly significant for most of the species. These taxa comprised primarily of clostridial species belonging to Clostridia clusters XIVa and IV and Bacteroidetes. The phylogenetic tree analysis revealed that none of the clostridial species that were significantly reduced in the gut microbiota of patients with MS overlapped with other spore-forming clostridial species capable of inducing colonic regulatory T cells (Treg), which prevent autoimmunity and allergies; this suggests that many of the clostridial species associated with MS might be distinct from those broadly associated with autoimmune conditions. Correcting the dysbiosis and altered gut microbiota might deserve consideration as a potential strategy for the prevention and treatment of MS.

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  • Excretion of 3,3 ',4,4 ',5-Pentachlorobiphenyl (PCB126) from Rat Liver Following Oral Administration of Lactobacillus reuteri and Lactobacillus acidophilus 査読

    Takehito Suzuki, Kaora Yamazaki, Tadashi Shinoda, Mitsuyuki Shirai, Hiroshi Yoshikawa, Yurika Noguchi, Tetsuro Ito, Yasuo Ishii, Tatsuya Takizawa, Hidetoshi Morita

    FOOD SCIENCE AND TECHNOLOGY RESEARCH   20 ( 4 )   821 - 828   2014年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    Oral administration of Lactobacillus reuteri CP3012 or Lactobacillus acidophilus L-92 for 60 days in rats that were previously administered 3,3',4,4',5-pentachlorobiphenyl (PCB126) orally at a dose of 100 mu g/kg of body weight resulted in a significant decrease in hepatic bioaccumulation of PCB126 ( p &lt; 0.05), with levels of 30.7 +/- 3.7 ng/g and 92.6 +/- 25.0 ng/g of liver tissue, respectively, compared with 133.1 +/- 12.7 ng/g of liver tissue in the controls. The electron paramagnetic resonance signal level of the liver PCB126-specific g = 2.49 species in rats administered L. reuteri CP3012 decreased significantly (p &lt; 0.05). Both the bile acid concentration in the feces and total stool output increased significantly following administration of lactobacilli ( p &lt; 0.05); however, adsorption of PCB126 onto the bacterial cells was not observed. These results suggest that these bacteria inhibit reabsorption of PCB126 with bile acid by blocking enterohepatic circulation through absorbing and/or deconjugating the bile acids in the intestinal tract and by promoting excretion of bile acids from the body, thus reducing PCB126 accumulation in the liver.

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  • Mariner-based transposon mutagenesis for Bacteroides species 査読

    Minoru Ichimura, Keiko Uchida, Haruyuki Nakayama-Imaohji, Hideki Hirakawa, Tomoyo Tada, Hidetoshi Morita, Koji Yasutomo, Katsuichiro Okazaki, Tomomi Kuwahara

    JOURNAL OF BASIC MICROBIOLOGY   54 ( 6 )   558 - 567   2014年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Bacteroides is one of the most predominant groups of human gut microbiota. Recent metagenomic analyses and studies on gnotobiotic mice demonstrated the tight association of Bacteroides with epithelial function, the gut immune system and systemic metabolism in the host. The mariner family transposon shows relatively low target site specificity and has hosts ranging from prokaryotes to eukaryotes. Thereby, random mutagenesis using the mariner family transposon is expected to identify key molecules for human-Bacteroides symbiosis. In this study, we constructed the plasmid pMI07 to deliver the gene cassette (ermF/ITR), which harbors the erythromycin resistant marker (ermF) and the inverted repeat sequences (ITRs) recognized by Himar1 transposase, to Bacteroides via electrotransformation. pMI07 successfully delivered ermF/ITR to the Bacteroides genomes and generated thousands of insertion mutants/mu g of pMI07 in B. thetaiotaomicron, B. fragilis, B. ovatus, and also, although to a lesser extent, B. vulgatus. Analyses of the ermF/ITR insertion sites in B. thetaiotaomicron and B. vulgatus revealed that the cassette targeted the dinucleotide TA and integrated into the genomes in an unbiased manner. The data reported here will provide useful information for transposon mutagenesis in Bacteroides species, which will enable identification of the genes responsible for their unique phenotypes.

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  • Dysbiosis of Salivary Microbiota in Inflammatory Bowel Disease and Its Association With Oral Immunological Biomarkers 査読

    Heba S. Said, Wataru Suda, Shigeki Nakagome, Hiroshi Chinen, Kenshiro Oshima, Sangwan Kim, Ryosuke Kimura, Atsushi Iraha, Hajime Ishida, Jiro Fujita, Shuhei Mano, Hidetoshi Morita, Taeko Dohi, Hiroki Oota, Masahira Hattori

    DNA RESEARCH   21 ( 1 )   15 - 25   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Analysis of microbiota in various biological and environmental samples under a variety of conditions has recently become more practical due to remarkable advances in next-generation sequencing. Changes leading to specific biological states including some of the more complex diseases can now be characterized with relative ease. It is known that gut microbiota is involved in the pathogenesis of inflammatory bowel disease (IBD), mainly Crohn's disease and ulcerative colitis, exhibiting symptoms in the gastrointestinal tract. Recent studies also showed increased frequency of oral manifestations among IBD patients, indicating aberrations in the oral microbiota. Based on these observations, we analyzed the composition of salivary microbiota of 35 IBD patients by 454 pyrosequencing of the bacterial 16S rRNA gene and compared it with that of 24 healthy controls (HCs). The results showed that Bacteroidetes was significantly increased with a concurrent decrease in Proteobacteria in the salivary microbiota of IBD patients. The dominant genera, Streptococcus, Prevotella, Neisseria, Haemophilus, Veillonella, and Gemella, were found to largely contribute to dysbiosis (dysbacteriosis) observed in the salivary microbiota of IBD patients. Analysis of immunological biomarkers in the saliva of IBD patients showed elevated levels of many inflammatory cytokines and immunoglobulin A, and a lower lysozyme level. A strong correlation was shown between lysozyme and IL-1 beta levels and the relative abundance of Streptococcus, Prevotella, Haemophilus and Veillonella. Our data demonstrate that dysbiosis of salivary microbiota is associated with inflammatory responses in IBD patients, suggesting that it is possibly linked to dysbiosis of their gut microbiota.

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  • Complete sequence analysis of two cryptic plasmids from Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from healthy infant feces. 査読

    Takahata M, Toh H, Nakano A, Takagi M, Murakami M, Ishii Y, Takizawa T, Tanabe S, Morita H

    Animal science journal = Nihon chikusan Gakkaiho   85 ( 2 )   158 - 163   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/asj.12095

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  • Obesity-induced gut microbial metabolite promotes liver cancer through senescence secretome (vol 499, pg 97, 2013) 査読

    Shin Yoshimoto, Tze Mun Loo, Koji Atarashi, Hiroaki Kanda, Seidai Sato, Seiichi Oyadomari, Yoichiro Iwakura, Kenshiro Oshima, Hidetoshi Morita, Masahira Hattori, Kenya Honda, Yuichi Ishikawa, Eiji Hara, Naoko Ohtani

    NATURE   506 ( 7488 )   396   2014年2月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    DOI: 10.1038/nature13004

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  • Anti-inflammatory and intestinal barrier-protective activities of commensal lactobacilli and bifidobacteria in thoroughbreds: Role of probiotics in diarrhea prevention in neonatal Thoroughbreds 査読

    Soichi Tanabe, Takuya Suzuki, Yuichiro Wasano, Fumihiko Nakajima, Hiroshi Kawasaki, Tomonori Tsuda, Natsuko Nagamine, Takashi Tsurumachi, Kiyoshi Sugaya, Hiroaki Akita, Misako Takagi, Kunihiko Takagi, Yoshinobu Inoue, Yo Asai, Hidetoshi Morita

    Journal of Equine Science   25 ( 2 )   37 - 43   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japanese Society of Equine Science  

    We previously isolated the commensal bacteria lactobacilli and bifidobacteria from the Thoroughbred intestine and prepared the horse probiotics LacFi™, consisting of Lactobacillus ruminis KK14, L. equi KK 15, L. reuteri KK18, L. johnsonii KK21, and Bifidobacterium boum HU. Here, we found that the fve LacFi™ constituent strains remarkably suppressed pro-inflammatory interleukin-17 production in mouse splenocytes stimulated with interleukin-6 and transforming growth factor-β. The protective effects of the probiotic on impaired intestinal barrier function were evaluated in Caco-2 cells treated with tumor necrosis factor-α. Evaluation of transepithelial resistance showed that all the strains exhibited intestinal barrier protective activity, with significant suppression of barrier impairment by L. reuteri KK18. The LacFi™ constituent strains were detected in neonatal LacFi™-administered Thoroughbred feces using polymerase chain reaction denaturing gradient gel electrophoresis and culture methods. These fve strains were found to be the predominant lactobacilli and bifidobacteria in the intestinal microbiota of LacFi™-administered Thoroughbreds. Administration of LacFi™ to neonatal Thoroughbreds decreased diarrhea incidence from 75.9% in the control group (n=29 neonatal Thoroughbreds) to 30.7% in the LacFi™-administered group (n=101 neonatal Thoroughbreds) immediately after birth to 20 weeks after birth. LacFi™ treatment also prevented diarrhea especially at and around 4 weeks and from 10 to 16 weeks. The duration of diarrhea was also shorter in the probiotics-administered group (7.4 ± 0.8 days) than in the control group (14.0 ± 3.2 days). These results indicate that the LacFi™ probiotics regulates intestinal function and contributes to diarrhea prevention. © 2014 Japanese Society of Equine Science.

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  • Bifidobacterium longum Alleviates Dextran Sulfate Sodium-Induced Colitis by Suppressing IL-17A Response: Involvement of Intestinal Epithelial Costimulatory Molecules 査読

    Eiji Miyauchi, Tasuku Ogita, Junki Miyamoto, Seiji Kawamoto, Hidetoshi Morita, Hiroshi Ohno, Takuya Suzuki, Soichi Tanabe

    PLOS ONE   8 ( 11 )   e79735   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Although some bacterial strains show potential to prevent colitis, their mechanisms are not fully understood. Here, we investigated the anti-colitic mechanisms of Bifidobacterium longum subsp. infantis JCM 1222(T), focusing on the relationship between interleukin (IL)-17A secreting CD4(+) T cells and intestinal epithelial costimulatory molecules in mice. Oral administration of JCM 1222(T) to mice alleviated dextran sulfate sodium (DSS)-induced acute colitis. The expression of type 1 helper T (Th1)- and IL-17 producing helper T (Th17)-specific cytokines and transcriptional factors was suppressed by JCM 1222(T) treatment. Intestinal epithelial cells (IECs) from colitic mice induced IL-17A production from CD4(+) T cells in a cell-cell contact-dependent manner, and this was suppressed by oral treatment with JCM 1222(T). Using blocking antibodies for costimulatory molecules, we revealed that epithelial costimulatory molecules including CD80 and CD40, which were highly expressed in IECs from colitic mice, were involved in IEC-induced IL-17A response. Treatment of mice and intestinal epithelial cell line Colon-26 cells with JCM 1222(T) decreased the expression of CD80 and CD40. Collectively, these data indicate that JCM 1222(T) negatively regulate epithelial costimulatory molecules, and this effect might be attributed, at least in part, to suppression of IL-17A in DSS-induced colitis.

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  • Genomic Adaptation of the Lactobacillus casei Group 査読

    Hidehiro Toh, Kenshiro Oshima, Akiyo Nakano, Muneaki Takahata, Masaru Murakami, Takashi Takaki, Hidetoshi Nishiyama, Shizunobu Igimi, Masahira Hattori, Hidetoshi Morita

    PLOS ONE   8 ( 10 )   e75073   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Lactobacillus casei, L. paracasei, and L. rhamnosus form a closely related taxonomic group (Lactobacillus casei group) within the facultatively heterofermentative lactobacilli. Here, we report the complete genome sequences of L. paracasei JCM 8130 and L. casei ATCC 393, and the draft genome sequence of L. paracasei COM0101, all of which were isolated from daily products. Furthermore, we re-annotated the genome of L. rhamnosus ATCC 53103 (also known as L. rhamnosus GG), which we have previously reported. We confirmed that ATCC 393 is distinct from other strains previously described as L. paracasei. The core genome of 10 completely sequenced strains of the L. casei group comprised 1,682 protein-coding genes. Although extensive genome-wide synteny was found among the L. casei group, the genomes of ATCC 53103, JCM 8130, and ATCC 393 contained genomic islands compared with L. paracasei ATCC 334. Several genomic islands, including carbohydrate utilization gene clusters, were found at the same loci in the chromosomes of the L. casei group. The spaCBA pilus gene cluster, which was first identified in GG, was also found in other strains of the L. casei group, but several L. paracasei strains including COM0101 contained truncated spaC gene. ATCC 53103 encoded a higher number of proteins involved in carbohydrate utilization compared with intestinal lactobacilli, and extracellular adhesion proteins, several of which are absent in other strains of the L. casei group. In addition to previously fully sequenced L. rhamnosus and L. paracasei strains, the complete genome sequences of L. casei will provide valuable insights into the evolution of the L. casei group.

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  • Complete Genome Sequence of the Equol-Producing Bacterium Adlercreutzia equolifaciens DSM 19450T 査読

    H. Toh, K. Oshima, T. Suzuki, M. Hattori, H. Morita

    Genome Announcements   1 ( 5 )   2013年9月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    DOI: 10.1128/genomea.00742-13

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  • T-reg induction by a rationally selected mixture of Clostridia strains from the human microbiota 査読

    Koji Atarashi, Takeshi Tanoue, Kenshiro Oshima, Wataru Suda, Yuji Nagano, Hiroyoshi Nishikawa, Shinji Fukuda, Takuro Saito, Seiko Narushima, Koji Hase, Sangwan Kim, Joelle V. Fritz, Paul Wilmes, Satoshi Ueha, Kouji Matsushima, Hiroshi Ohno, Bernat Olle, Shimon Sakaguchi, Tadatsugu Taniguchi, Hidetoshi Morita, Masahira Hattori, Kenya Honda

    NATURE   500 ( 7461 )   232 - +   2013年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Manipulation of the gut microbiota holds great promise for the treatment of inflammatory and allergic diseases(1,2). Although numerous probiotic microorganisms have been identified(3), there remains a compelling need to discover organisms that elicit more robust therapeutic responses, are compatible with the host, and can affect a specific arm of the host immune system in a well-controlled, physiological manner. Here we use a rational approach to isolate CD4(+)FOXP3(+) regulatory T (T-reg)-cell-inducing bacterial strains from the human indigenous microbiota. Starting with a healthy human faecal sample, a sequence of selection steps was applied to obtain mice colonized with human microbiota enriched in T-reg-cell-inducing species. From these mice, we isolated and selected 17 strains of bacteria on the basis of their high potency in enhancing T-reg cell abundance and inducing important anti-inflammatory molecules-including interleukin-10 (IL-10) and inducible T-cell co-stimulator (ICOS)-in T-reg cells upon inoculation into germ-free mice. Genome sequencing revealed that the 17 strains fall within clusters IV, XIVa and XVIII of Clostridia, which lack prominent toxins and virulence factors. The 17 strains act as a community to provide bacterial antigens and a TGF-beta-rich environment to help expansion and differentiation of T-reg cells. Oral administration of the combination of 17 strains to adult mice attenuated disease in models of colitis and allergic diarrhoea. Use of the isolated strains may allow for tailored therapeutic manipulation of human immune disorders.

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  • Obesity-induced gut microbial metabolite promotes liver cancer through senescence secretome 査読

    Shin Yoshimoto, Tze Mun Loo, Koji Atarashi, Hiroaki Kanda, Seidai Sato, Seiichi Oyadomari, Yoichiro Iwakura, Kenshiro Oshima, Hidetoshi Morita, Masahisa Hattori, Kenya Honda, Yuichi Ishikawa, Eiji Hara, Naoko Ohtani

    NATURE   499 ( 7456 )   97 - +   2013年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Obesity has become more prevalent in most developed countries over the past few decades, and is increasingly recognized as a major risk factor for several common types of cancer(1). As the worldwide obesity epidemic has shown no signs of abating(2), better understanding of the mechanisms underlying obesity-associated cancer is urgently needed. Although several events were proposed to be involved in obesity-associated cancer1,3, the exact molecular mechanisms that integrate these events have remained largely unclear. Here we show that senescence-associated secretory phenotype (SASP)(4,5) has crucial roles in promoting obesity-associated hepatocellular carcinoma (HCC) development in mice. Dietary or genetic obesity induces alterations of gut microbiota, thereby increasing the levels of deoxycholic acid (DCA), a gut bacterial metabolite known to cause DNA damage(6). The enterohepatic circulation of DCA provokes SASP phenotype in hepatic stellate cells (HSCs)(7), which in turn secretes various inflammatory and tumour-promoting factors in the liver, thus facilitating HCC development in mice after exposure to chemical carcinogen. Notably, blocking DCA production or reducing gut bacteria efficiently prevents HCC development in obese mice. Similar results were also observed in mice lacking an SASP inducer(8) or depleted of senescent HSCs, indicating that the DCA-SASP axis in HSCs has key roles in obesity-associated HCC development. Moreover, signs of SASP were also observed in the HSCs in the area of HCC arising in patients with non-alcoholic steatohepatitis(3), indicating that a similar pathway may contribute to at least certain aspects of obesity-associated HCC development in humans as well. These findings provide valuable new insights into the development of obesity-associated cancer and open up new possibilities for its control.

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  • Robustness of Gut Microbiota of Healthy Adults in Response to Probiotic Intervention Revealed by High-Throughput Pyrosequencing 査読

    Seok-Won Kim, Wataru Suda, Sangwan Kim, Kenshiro Oshima, Shinji Fukuda, Hiroshi Ohno, Hidetoshi Morita, Masahira Hattori

    DNA RESEARCH   20 ( 3 )   241 - 253   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Probiotics are live microorganisms that potentially confer beneficial outcomes to host by modulating gut microbiota in the intestine. The aim of this study was to comprehensively investigate effects of probiotics on human intestinal microbiota using 454 pyrosequencing of bacterial 16S ribosomal RNA genes with an improved quantitative accuracy for evaluation of the bacterial composition. We obtained 158 faecal samples from 18 healthy adult Japanese who were subjected to intervention with 6 commercially available probiotics containing either Bifidobacterium or Lactobacillus strains. We then analysed and compared bacterial composition of the faecal samples collected before, during, and after probiotic intervention by Operational taxonomic units (OTUs) and UniFrac distances. The results showed no significant changes in the overall structure of gut microbiota in the samples with and without probiotic administration regardless of groups and types of the probiotics used. We noticed that 32 OTUs (2.7% of all analysed OTUs) assigned to the indigenous species showed a significant increase or decrease of &gt;= 10-fold or a quantity difference in &gt;150 reads on probiotic administration. Such OTUs were found to be individual specific and tend to be unevenly distributed in the subjects. These data, thus, suggest robustness of the gut microbiota composition in healthy adults on probiotic administration.

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  • Genomic Analysis by Deep Sequencing of the Probiotic Lactobacillus brevis KB290 Harboring Nine Plasmids Reveals Genomic Stability 査読

    Masanori Fukao, Kenshiro Oshima, Hidetoshi Morita, Hidehiro Toh, Wataru Suda, Seok-Won Kim, Shigenori Suzuki, Takafumi Yakabe, Masahira Hattori, Nobuhiro Yajima

    PLoS ONE   8 ( 3 )   e60521 - e60521   2013年3月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

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  • PhoB Regulates the Survival of Bacteroides fragilis in Peritoneal Abscesses 査読

    Shin Wakimoto, Haruyuki Nakayama-Imaohji, Minoru Ichimura, Hidetoshi Morita, Hideki Hirakawa, Tetsuya Hayashi, Koji Yasutomo, Tomomi Kuwahara

    PLOS ONE   8 ( 1 )   e53829   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    In response to phosphate limitation, bacteria employ the Pho regulon, a specific regulatory network for phosphate acquisition. The two-component signal transduction system of PhoRB plays a crucial role in the induction of Pho regulon genes, leading to the adaptation to phosphate starvation. Herein, we identified the PhoRB system in Bacteroides fragilis, a commensal gut bacterium, and evaluated its role in gut colonization and survival in peritoneal abscesses. BF1575 and BF1576 encoded PhoR (sensor histidine kinase) and PhoB (response regulator) in the sequenced B. fragilis strain YCH46, respectively. Transcriptome analysis revealed that deletion of phoB affected the expression of 585 genes (more than 4-fold change) in B. fragilis, which included genes for stress response (chaperons and heat shock proteins), virulence (capsular polysaccharide biosynthesis) and phosphate metabolism. Deletion of phoB reduced the ability of the bacterium to persist in peritoneal abscesses induced by an intra-abdominal challenge of B. fragilis. Furthermore, PhoB was necessary for survival of this anaerobe in peritoneal abscesses but not for in vitro growth in rich media or in intestinal colonization. These results indicate that PhoB plays an important role in the survival of B. fragilis under stressful extraintestinal conditions.

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  • Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro 査読

    Hideaki Abe, Wataru Ishikawa, Takahiro Kushima, Tomoka Nishimura, Chiemi Mori, Atsushi Onuki, Takehito Suzuki, Yasuo Ishii, Norio Kansaku, Yoko Miyazaki, Kazuaki Tanaka, Hidetoshi Morita, Tatsuya Takizawa

    Bioscience, Biotechnology and Biochemistry   77 ( 5 )   971 - 976   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We investigated the role of nitric oxide (NO) in vascular endothelial growth factor (VEGF) expression in the rat placenta. A nitric oxide synthase (NOS) inhibitor, NG-nitro-L-arginine-methyl ester (L-NAME), was constantly infused into pregnant rats 6-24 h before sacrifice on gestational day (GD) 15.5. NO production declined to about 15% of the control level as monitored by NO trapping and electron paramagnetic resonance spectroscopy. VEGF mRNA expression was temporally decreased by L-NAME, but recovered to normal levels after 24 h of treatment, whereas hypoxia inducible factor (HIF)-1α and induced NOS (iNOS) expression increased. VEGF expression decreased significantly in placental explants after 6 h of co-treatment with LNAME and lipopolysaccharide, an iNOS inducer. Our data indicate that NO induce VEGF expression in vivo and in vitro in the rat placenta, suggesting that peaked NO production was maintained by a reciprocal relationship between NO and VEGF via HIF-1α.

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  • Aqueous extracts of Rhizopus oryzae induced nitric oxide production in rat hepatocyte cell line RLN-10 査読

    Takehito Suzuki, Mayuko Uchida, Yuji Takeda, Chiemi Mori, Atsushi Onuki, Yoko Miyazaki, Ken Onda, Setsuo Ushikoshi, Kotaro Shitori, Kazuaki Tanaka, Hidetoshi Morita, Tatsuya Takizawa

    Bioscience, Biotechnology and Biochemistry   77 ( 7 )   1384 - 1389   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aqueous extracts of Rhizopus oryzae (Aq-ROU) have a broad range of physiological activity. Here we identified a new physiological effect of Aq-ROU in rat hepatocyte cell line RLN-10. Aq-ROU induced the accumulation of nitrite, a stable metabolite nitric oxide (NO), in cell culture medium and induced potent diaminofluorescein-FM diacetate staining in the cells. Real-time reverse transcriptase (RT)-PCR analysis showed marked inducible NO synthase gene expression. Additionally, markedly enhanced expression of p22phox and temporally increased expression of NADPH oxidase1 indicated that superoxide was produced. Nuclear translocation of nuclear factor-kappa (NF-κ) B p65 increased remarkably following Aq-ROU and following lipopolysaccharide treatment, a potent activator of NF-κB. Ammonium pyrrolidine-1- carbodithioate, an inhibitor of NF-κB, inhibited NO production following Aq-ROU treatment. Our data indicate that Aq-ROU induces NO production and potentially the production of superoxide, which may contribute to the broad range of physiological effects observed for Aq-ROU ingested by animals.

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  • Effect of D-Alanine in Teichoic Acid from the Streptococcus thermophilus Cell Wall on the Barrier-Protection of Intestinal Epithelial Cells 査読

    Eiji Miyauchi, Maho Morita, Mauro Rossi, Hidetoshi Morita, Takuya Suzuki, Soichi Tanabe

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   76 ( 2 )   283 - 288   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258(T) on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258(T) suppressed the tumor necrosis factor-alpha-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258(T) was growth phase- and culture temperature-dependent. Treatment of ATCC 19258(T) with Mg2+ decreased the dlt mRNA expression and DAla content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with L-alanine (L-Ala) to the broth led to an increase of L-Ala in ATCC 19258(T) and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.

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  • Comparative Genomic Analysis ofLactococcus garvieaeStrains Isolated from Different Sources Reveals Candidate Virulence Genes 査読

    Eiji Miyauchi, Hidehiro Toh, Akiyo Nakano, Soichi Tanabe, Hidetoshi Morita

    International Journal of Microbiology   2012   1 - 7   2012年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Hindawi Limited  

    <italic>Lactococcus garvieae</italic>is a major pathogen for fish. Two complete (ATCC 49156 and Lg2) and three draft (UNIUD074, 8831, and 21881) genome sequences of<italic>L. garvieae</italic>have recently been released. We here present the results of a comparative genomic analysis of these fish and human isolates of<italic>L. garvieae</italic>. The pangenome comprised 1,542 core and 1,378 dispensable genes. The sequenced<italic>L. garvieae</italic>strains shared most of the possible virulence genes, but the capsule gene cluster was found only in fish-pathogenic strain Lg2. The absence of the capsule gene cluster in other nonpathogenic strains isolated from mastitis and vegetable was also confirmed by PCR. The fish and human isolates of<italic>L. garvieae</italic>contained the specific two and four adhesin genes, respectively, indicating that these adhesion proteins may be involved in the host specificity differences of<italic>L. garvieae</italic>. The discoveries revealed by the pangenomic analysis may provide significant insights into the biology of<italic>L. garvieae</italic>.

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  • Assessment and Improvement of Methods for Microbial DNA Preparation from Fecal Samples 査読

    Mariko Ueno, Mami Kikuchi, Kenshiro Oshima, Seok-Won Kim, Hidetoshi Morita, Masahira Hattori

    Handbook of Molecular Microbial Ecology II: Metagenomics in Different Habitats   191 - 198   2011年11月

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    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:John Wiley and Sons  

    DOI: 10.1002/9781118010549.ch19

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  • Bifidobacterium kashiwanohense sp nov., isolated from healthy infant faeces 査読

    Hidetoshi Morita, Akiyo Nakano, Hiromi Onoda, Hidehiro Toh, Kenshiro Oshima, Hideto Takami, Masaru Murakami, Shinji Fukuda, Tatsuya Takizawa, Tomomi Kuwahara, Hiroshi Ohno, Soichi Tanabe, Masahira Hattori

    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY   61 ( Pt 11 )   2610 - 2615   2011年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Strains HM2-1 and HM2-2(T) were isolated from the faeces of a healthy infant and were characterized by determining their phenotypic and biochemical features and phylogenetic positions based on partial 16S rRNA gene sequence analysis. They were Gram-positive, obligately anaerobic, non-spore-forming, non-gas-producing, and catalase-negative non-motile rods. They did not grow at 15 or 45 degrees C in anaerobic bacterial culture medium, and their DNA G+C content was in the range 56-59 molok. In enzyme activity tests, strains HM2-1 and HM2-2(T) were positive for alpha/beta-galactosidases and alpha/beta-glucosidases but negative for beta-glucuronidase and cystine arylamidase. An analysis of the cell-wall composition of strains HM2-1 and HM2-2T revealed the presence of glutamic acid, alanine and lysine. The presence of fructose-6-phosphate phosphoketolase shows that isolates HM2-1 and HM2-2(T) are members of the genus Bifidobacterium. These two isolates belong to the same species of the genus Bifidobacterium. Strain HM2-2(T) was found to be related to Bifidobacterium catenulatum JCM 1194(T) (97.4% 16S rRNA gene sequence identity: 1480/1520 bp), Bifidobacterium pseudocatenulatum JCM 1200(T) (97.2%: 1472/1514 bp), Bifidobacterium dentium ATCC 27534(T) (96.7 %: 1459/1509 bp) and Bifidobacterium angulatum ATCC 27535(T) (965%: 1462/1515 bp). The predominant cellular fatty acids of strains HM2-1 and HM2-2(T) were 16:0 and 18:1 omega 9c, with proportions greater than 18% of the total. Phylogenetic analyses involving phenotypic characterization, DNA-DNA hybridization and partial 16S rRNA gene sequencing proves that the strains represent a novel species of the genus Bifidobacterium, for which the name Bifidobacterium kashiwanohense sp. nov. is proposed. The type strain is HM2-2(T) (=JCM 15439(T) =DSM 21854(T)).

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  • Suppression of Th17 response by Streptococcus thermophilus ST28 through induction of IFN-γ. 査読

    Ogita T, Tanii Y, Morita H, Suzuki T, Tanabe S

    International journal of molecular medicine   28 ( 5 )   817 - 822   2011年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Complete Genome Sequences of Rat and Mouse Segmented Filamentous Bacteria, a Potent Inducer of Th17 Cell Differentiation 査読

    Tulika Prakash, Kenshiro Oshima, Hidetoshi Morita, Shinji Fukuda, Akemi Imaoka, Naveen Kumar, Vineet K. Sharma, Seok-Won Kim, Mahoko Takahashi, Naruya Saitou, Todd D. Taylor, Hiroshi Ohno, Yoshinori Umesaki, Masahira Hattori

    CELL HOST & MICROBE   10 ( 3 )   273 - 284   2011年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CELL PRESS  

    Segmented filamentous bacteria (SFB) are noncultivable commensals inhabiting the gut of various vertebrate species and have been shown to induce Th17 cells in mice. We present the complete genome sequences of both rat and mouse SFB isolated from SFB-monocolonized hosts. The rat and mouse SFB genomes each harbor a single circular chromosome of 1.52 and 1.59 Mb encoding 1346 and 1420 protein-coding genes, respectively. The overall nucleotide identity between the two genomes is 86%, and the substitution rate was estimated to be similar to that of the free-living E. coli. SFB genomes encode typical genes for anaerobic fermentation and spore and flagella formation, but lack most of the amino acid biosynthesis enzymes, reminiscent of pathogenic Clostridia, exhibiting large dependency on the host. However, SFB lack most of the clostridial virulence-related genes. Comparative analysis with clostridial genomes suggested possible mechanisms for host responses and specific adaptations in the intestine.

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  • Complete Genome Sequence and Comparative Analysis of the Fish Pathogen Lactococcus garvieae 査読

    Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Mariko Yoshizaki, Michiko Kawanishi, Kohei Nakaya, Takehito Suzuki, Eiji Miyauchi, Yasuo Ishii, Soichi Tanabe, Masaru Murakami, Masahira Hattori

    PLOS ONE   6 ( 8 )   e23184   2011年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Lactococcus garvieae causes fatal haemorrhagic septicaemia in fish such as yellowtail. The comparative analysis of genomes of a virulent strain Lg2 and a non-virulent strain ATCC 49156 of L. garvieae revealed that the two strains shared a high degree of sequence identity, but Lg2 had a 16.5-kb capsule gene cluster that is absent in ATCC 49156. The capsule gene cluster was composed of 15 genes, of which eight genes are highly conserved with those in exopolysaccharide biosynthesis gene cluster often found in Lactococcus lactis strains. Sequence analysis of the capsule gene cluster in the less virulent strain L. garvieae Lg2-S, Lg2-derived strain, showed that two conserved genes were disrupted by a single base pair deletion, respectively. These results strongly suggest that the capsule is crucial for virulence of Lg2. The capsule gene cluster of Lg2 may be a genomic island from several features such as the presence of insertion sequences flanked on both ends, different GC content from the chromosomal average, integration into the locus syntenic to other lactococcal genome sequences, and distribution in human gut microbiomes. The analysis also predicted other potential virulence factors such as haemolysin. The present study provides new insights into understanding of the virulence mechanisms of L. garvieae in fish.

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  • Bifidobacteria can protect from enteropathogenic infection through production of acetate 査読

    Shinji Fukuda, Hidehiro Toh, Koji Hase, Kenshiro Oshima, Yumiko Nakanishi, Kazutoshi Yoshimura, Toru Tobe, Julie M. Clarke, David L. Topping, Tohru Suzuki, Todd D. Taylor, Kikuji Itoh, Jun Kikuchi, Hidetoshi Morita, Masahira Hattori, Hiroshi Ohno

    NATURE   469 ( 7331 )   543 - U791   2011年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    The human gut is colonized with a wide variety of microorganisms, including species, such as those belonging to the bacterial genus Bifidobacterium, that have beneficial effects on human physiology and pathology(1-3). Among the most distinctive benefits of bifidobacteria are modulation of host defence responses and protection against infectious diseases(4-6). Nevertheless, the molecular mechanisms underlying these effects have barely been elucidated. To investigate these mechanisms, we used mice associated with certain bifidobacterial strains and a simplified model of lethal infection with enterohaemorrhagic Escherichia coli O157:H7, together with an integrated &apos;omics&apos; approach. Here we show that genes encoding an ATP-binding-cassette-type carbohydrate transporter present in certain bifidobacteria contribute to protecting mice against death induced by E. coli O157:H7. We found that this effect can be attributed, at least in part, to increased production of acetate and that translocation of the E. coli O157:H7 Shiga toxin from the gut lumen to the blood was inhibited. We propose that acetate produced by protective bifidobacteria improves intestinal defence mediated by epithelial cells and thereby protects the host against lethal infection.

    DOI: 10.1038/nature09646

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  • Streptococcus thermophilus ST28 Ameliorates Colitis in Mice Partially by Suppression of Inflammatory Th17 Cells 査読

    Tasuku Ogita, Megumi Nakashima, Hidetoshi Morita, Yasuo Saito, Takuya Suzuki, Soichi Tanabe

    JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY   2011   378417   2011年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:HINDAWI PUBLISHING CORPORATION  

    The effects of Streptococcus thermophilus ST28 on cytokine production by murine splenocytes stimulated with transforming growth factor-beta plus interleukin- (IL-) 6 were evaluated. The addition of ST28 significantly repressed IL-17 production compared to ATCC 19258 (type strain). ST28 also decreased the number of Th17 cells in the stimulated splenocytes. The anti-inflammatory effects of ST28 administration were evaluated in mice with colitis induced by dextran sodium sulphate (DSS). Oral treatment of mice with ST28 ameliorated the intestinal lesions by DSS. Upon DSS treatment, IL-17 production in lamina propria lymphocytes (LPLs) was induced, but ST28 significantly decreased its production. ST28 also decreased the percentage of Th17 cells in LPL from DSS-induced colitis. The present results imply that ST28 suppresses the Th17 response in inflamed intestines and would be useful in the treatment of Th17-mediated diseases, such as inflammatory bowel disease.

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  • Nitric Oxide Production and its Contribution to Hepatocyte Proliferation in Normal Juvenile Rats 査読

    Naoto Inukai, Mayuko Uchida, Yoko Miyazaki, Takehito Suzuki, Hiroshi Yoshikawa, Kazuaki Tanaka, Hidetoshi Morita, Tatsuya Takizawa

    JOURNAL OF VETERINARY MEDICAL SCIENCE   72 ( 7 )   861 - 867   2010年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC VET SCI  

    Nitric oxide (NO) has been reported as a key mediator in enhancing hepatocyte proliferation during liver regeneration. Juvenile hepatocytes have a strong ability to proliferate while still in their undifferentiated state but the mechanism of NO production and its contribution to hepatocyte proliferation are not yet fully understood. The present study was designed to investigate NO production in the normal liver and its contribution to hepatocyte proliferation in juvenile rats. Endogenous NO production was evaluated quantitatively using a spin trap followed by electron paramagnetic resonance spectroscopy with the Fe-N, N-diethyldithiocarbamate complex as an NO-trapping reagent in the rat liver. NO production in the liver significantly peaked at 3 weeks after birth, but NO synthase (NOS) 3 expression did not change between 2 to 5 weeks after birth, while NOS 1 and NOS 2 mRNA were not detected. Hepatocyte proliferation, measured by the incorporation of 5-bromo-2'-deoxyuridine into the DNA, was found to decline significantly when endogenous NO production was inhibited by the administration of the NOS inhibitor N-G-nitro-L-arginine methyl ester. These findings indicate that endogenous NO production peaked at 3 weeks after birth and hepatocyte proliferation declined significantly when NO production was inhibited. Thus, this study provides a novel insight into the contribution of NO to hepatic growth and liver maturation in juveniles.

    DOI: 10.1292/jvms.09-0551

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  • Efficient Electrotransformation of Bacteroides fragilis 査読

    Minoru Ichimura, Haruyuki Nakayama-Imaohji, Shin Wakimoto, Hidetoshi Morita, Tetsuya Hayashi, Tomomi Kuwahara

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   76 ( 10 )   3325 - 3332   2010年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    This study describes refined electroporation parameters for efficient transformation of Bacteroides fragilis by plasmids prepared from laboratory strains of Escherichia coli. Development of the method used included determination of the optimal growth conditions for competent cell preparation, selectable antimicrobial resistance markers, electric field strength, and postpulse incubation time. Of the four E. coli-Bacteroides shuttle plasmids tested (pVAL-1, pVAL-2, pNLY1, and pLYL05), pLYL05 containing the cefoxitin resistance marker was found to be the most suitable for B. fragilis transformation, and it generated 2- to 900-fold more transformants (about 10(4) transformants per mu g pLYL05 DNA) than the other plasmids. For the 72-h cultivation period tested, B. fragilis cells harvested at 48 h yielded the highest numbers of transformants. The transformation efficiency of pLYL05 increased linearly with the electric field strength over a range from 5.0 to 12.5 kV/cm. At least 3 h of postpulse incubation was required to maximize the transformation efficiency. For deletion of B. fragilis genes by homologous recombination, competent cells grown to early exponential phase and 12 h of postpulse incubation were required for efficient integration of the pLYL05-based suicide vector into the target site. The expected integration was obtained in B. fragilis strain NCTC9343 only when a homologously prepared (i.e., in vivo methylated) suicide vector was used. Spontaneous resolution of the diploid successfully deleted the expected genetic region. Our simple and efficient plasmid transfer method enabled disruption of a B. fragilis gene using in vivo-methylated targeted vectors. Our optimized electroporation parameters provide a useful tool for genetic manipulation of Bacteroides species.

    DOI: 10.1128/AEM.02420-09

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  • Apoptosis caused by an inhibitor of NO production in the decidua of rat from mid-gestation 査読

    Takehito Suzuki, Chiaki Nagamatsu, Takahiro Kushima, Ryu Miyakoshi, Kazuaki Tanaka, Hidetoshi Morita, Motoharu Sakaue, Tatsuya Takizawa

    EXPERIMENTAL BIOLOGY AND MEDICINE   235 ( 4 )   455 - 462   2010年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC EXPERIMENTAL BIOLOGY MEDICINE  

    We previously reported that nitric oxide (NO) is first detected in the uterus of a pregnant rat on gestational day 13.5 (GD13.5) and that NO levels peak on GD17.5. In addition, NO production in the uterus is mainly derived from the decidua and not the myometrium. The aim of the present study was to reveal the role of NO that peaked on GD17.5 of gestation in the decidua. To inhibit NO production, pregnant rats were continuously administered by an nitric oxide synthase inhibitor, N(G)-nitro-L-arginine-methyl ester (L-NAME) for 48 h. In the control group, saline was infused instead of L-NAME. After treatment, the decidua were obtained from GD13.5, GD17.5 and GD21.5 rats. Apoptosis and activated caspase-3-positive cells were observed by transferase-mediated dUTP nick-end labeling (TUNEL) assay and immunohistochemistry, respectively. The caspase-3 enzyme activity was also measured in the cell lysate from the decidua. The numbers of TUNEL-positive cells and activated caspase-3-positive cells each increased and the amount of caspase-3 activity also increased significantly in rats on GD17.5 than in rats in the control group, but no changes were observed in rats on GD13.5 and GD21.5. Furthermore, enzyme activity regarding the initiator caspases, caspase-8 and -9, upstream factors for caspase-3 in the caspase cascade, was measured simultaneously on GD17.5 under the same treatment. Caspase-8 and -9 enzyme activities increased significantly in the control group; an increment of caspase-8 activity was especially prominent. The present results indicate that an inhibitor of NO production caused apoptosis through typical apoptotic signals in the decidua on GD17.5, suggesting that an NO peak in the decidua is essential to cell survival and the maintenance of uterine formation.

    DOI: 10.1258/ebm.2009.009285

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  • Complete Genome Sequence of the Wild-Type Commensal Escherichia coli Strain SE15, Belonging to Phylogenetic Group B2 査読

    Hidehiro Toh, Kenshiro Oshima, Atsushi Toyoda, Yoshitoshi Ogura, Tadasuke Ooka, Hiroyuki Sasamoto, Sang-Hee Park, Sunao Iyoda, Ken Kurokawa, Hidetoshi Morita, Kikuji Itoh, Todd D. Taylor, Tetsuya Hayashi, Masahira Hattori

    JOURNAL OF BACTERIOLOGY   192 ( 4 )   1165 - 1166   2010年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Escherichia coli SE15 (O150:H5) is a human commensal bacterium recently isolated from feces of a healthy adult and classified into E. coli phylogenetic group B2, which includes the majority of extraintestinal pathogenic E. coli. Here, we report the finished and annotated genome sequence of this organism.

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  • Lactobacillus equicursoris sp nov., isolated from the faeces of a thoroughbred racehorse 査読

    Hidetoshi Morita, Mitsuharu Shimazu, Hiroshi Shiono, Hidehiro Toh, Fumihiko Nakajima, Hiroaki Akita, Misako Takagi, Hideto Takami, Masaru Murakami, Toshio Masaoka, Soichi Tanabe, Masahira Hattori

    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY   60 ( Pt 1 )   109 - 112   2010年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    We previously isolated five strains of putative lactobacilli from the faeces of a thoroughbred horse (a 4-year-old male). Of the five strains, four were identified as members of existing Lactobacillus species; however, sequence analysis of the 16S rRNA gene revealed that the fifth isolate, DI70(T) showed approximately 97% identity (1325/1366 bp) with the type strain of Lactobacillus delbrueckii. Therefore, we considered the possibility that DI70(T) represents a novel species of the genus Lactobacillus. Cells of strain DI70(T) were Gram-stain-positive, catalase-negative, non-spore-forming, non-motile rods. In phylogenetic trees constructed on the basis of 16S rRNA gene sequences, strain DI70(T) formed a subcluster in the L. delbrueckii phylogenetic group and was closely related to L. delbrueckii, Lactobacillus crispatus and Lactobacillus jensenii. However, analysis of DNA-DNA relatedness showed that DI70(T) was genetically distinct from its phylogenetic relatives. The isolate also exhibited distinct biochemical and physiological characteristics when compared with its phylogenetic relatives. It required anaerobic conditions for growth on agar medium. The results indicate that isolate DI70(T) indeed represents a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus equicursoris sp. nov. The type strain is DI70(T) (=JCM 14600(T) =DSM 19284(T)).

    DOI: 10.1099/ijs.0.009290-0

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  • Complete Genome Sequence of the Probiotic Lactobacillus rhamnosus ATCC 53103 査読

    Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Masaru Murakami, Todd D. Taylor, Shizunobu Igimi, Masahira Hattori

    JOURNAL OF BACTERIOLOGY   191 ( 24 )   7630 - 7631   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Lactobacillus rhamnosus is a facultatively heterofermentative lactic acid bacterium and is frequently isolated from human gastrointestinal mucosa of healthy individuals. L. rhamnosus ATCC 53103, isolated from a healthy human intestinal flora, is one of the most widely used and well-documented probiotics. Here, we report the finished and annotated genome sequence of this organism.

    DOI: 10.1128/JB.01287-09

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  • Changes in Nitric Oxide Production Levels and Expression of Nitric Oxide Synthase Isoforms in the Rat Uterus during Pregnancy 査読

    Takehito Suzuki, Chiemi Mori, Hiroshi Yoshikawa, Yoko Miyazaki, Norio Kansaku, Kazuaki Tanaka, Hidetoshi Morita, Tatsuya Takizawa

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   73 ( 10 )   2163 - 2166   2009年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    We clarified nitric oxide (NO) production in the rat uterus by electron paramagnetic resonance spectroscopy and with Fe-N-(dithiocarboxy) sarcosine complex (an NO-trapping reagent). We examined changes in NO production in the whole uterus, decidua, and myometrium (gestational days 13.5-2.1.5). The expression of nitric oxide synthase (NOS) isoforms was also examined by quantitative reverse transcription-polymerase chain reaction. The uterine NO levels were low on day 13.5, peaked on day 17.5, and thereafter decreased significantly. The NO production levels in the decidua and myometrium were the same on day 13.5, but the levels in the decidua were 2- to 4-fold higher than those in the myometrium from day 15.5 onwards. The NOS-2 mRNA expression pattern correlated well with changes in the NO levels in the decidua, whereas the NOS-3 mRNA was expressed constantly during gestation. Thus NOS-2-generated NO in the decidua contributed significantly to uterine NO levels.

    DOI: 10.1271/bbb.90222

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  • Lactobacillus hayakitensis, L-equigenerosi and L-equi, predominant lactobacilli in the intestinal flora of healthy thoroughbreds 査読

    Hidetoshi Morita, Akiyo Nakano, Mitsuharu Shimazu, Hidehiro Toh, Fumihiko Nakajima, Masahiro Nagayama, Shin Hisamatsu, Yukio Kato, Misako Takagi, Hideto Takami, Hiroaki Akita, Minoru Matsumoto, Toshio Masaoka, Masaru Murakami

    ANIMAL SCIENCE JOURNAL   80 ( 3 )   339 - 346   2009年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    To detect the predominant lactobacilli in the intestinal flora of healthy thoroughbreds, we isolated lactobacilli from the feces of nine thoroughbreds (five males and four females; 0-15-year-old). The isolated lactobacilli comprise 17 species (37 strains), and they were classified into five groups: Lactobacillus salivarius (6 species), L. reuteri (6 species), Lactobacillus delbrueckii (3 species), L. buchneri (1 species) and L. vitulinus (1 species). On the basis of 16S rRNA gene sequences, we identified 3 other phylogenetic relatives belonging to the genus Lactobacillus. These results suggest that the intestinal flora of thoroughbreds may comprise many species of the genus Lactobacillus. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analyses of the 340-bp fragments of the 16S rRNA genes from the same nine fecal samples showed that L. hayakitensis, L. equigenerosi and L. equi are contained in all the samples, suggesting that these species are predominant lactobacilli in the intestinal flora of thoroughbreds.

    DOI: 10.1111/j.1740-0929.2009.00633.x

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  • Gestational Changes in Production of NO and Expression of NOS mRNA Isoforms in the Rat Placenta 査読

    Takehito Suzuki, Yoshinori Ikeda, Hiroshi Yoshikawa, Kazuaki Tanaka, Hidetoshi Morita, Masako Yamamoto, Tatsuya Takizawa

    JOURNAL OF VETERINARY MEDICAL SCIENCE   71 ( 4 )   495 - 498   2009年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC VET SCI  

    To monitor changes in NO production over time in the fetal placenta of rats, we used electron paramagnetic resonance spectroscopy with the Fe-N-(dithiocarboxy) sarcosine (Fe-DTCS) complex as an NO-trapping reagent. The expression of nitric oxide synthase (NOS) isoforms was examined in parallel using quantitative RT-PCR. NO production was first detected on day 13.5 of gestation. NO levels reached a peak on day 15.5, then decreased significantly during the last few days of gestation. The pattern of expression of NOS II mRNA was in good agreement with changes in NO levels, whereas NOS III mRNA expression did not change markedly during gestation. Thus, it appears that NO levels in the placenta are NOS II-dependent and differ at different gestational stages.

    DOI: 10.1292/jvms.71.495

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  • Complete Genome Sequence and Comparative Analysis of the Wild-type Commensal Escherichia coli Strain SE11 Isolated from a Healthy Adult 査読

    Kenshiro Oshima, Hidehlro Toh, Yoshitoshi Ogura, Hiroyuki Sasamoto, Hidetoshi Morita, Sang-Hee Park, Tadasuke Ooka, Sunao Iyoda, Todd D. Taylor, Tetsuya Hayashi, Kikuji Itoh, Masahira Hattori

    DNA RESEARCH   15 ( 6 )   375 - 386   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    We sequenced and analyzed the genome of a commensal Escherichia coli (E. coli) strain SE11 (O152:H28) recently isolated from feces of a healthy adult and classified into E. coli phylogenetic group B1. SE11 harbored a 4.8 Mb chromosome encoding 4679 protein-coding genes and six plasmids encoding 323 protein-coding genes. None of the SE11 genes had sequence similarity to known genes encoding phage- and plasmid-borne virulence factors found in pathogenic E. coli strains. The comparative genome analysis with the laboratory strain K-12 MG 1655 identified 62 poorly conserved genes between these two non-pathogenic strains and 1186 genes absent in MG1655. These genes in SE11 were mostly encoded in large insertion regions on the chromosome or in the plasmids, and were notably abundant in genes of fimbriae and autotransporters, which are cell surface appendages that largely contribute to the adherence ability of bacteria to host cells and bacterial conjugation. These data suggest that SE11 may have evolved to acquire and accumulate the functions advantageous for stable colonization of intestinal cells, and that the adhesion-associated functions are important for the commensality of E. coli in human gut habitat.

    DOI: 10.1093/dnares/dsn026

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  • Sharpea azabuensis gen. nov., sp nov., a Gram-positive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses 査読

    Hidetoshi Morita, Chiharu Shiratori, Masaru Murakami, Hideto Takami, Hidehiro Toh, Yukio Kato, Fumihiko Nakajima, Misako Takagi, Hiroaki Akita, Toshio Masaoka, Masahira Hattori

    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY   58 ( Pt 12 )   2682 - 2686   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Four bacterial strains, designated ST18(T), HM244, HM250 and D149, were isolated from the fresh faeces of four thoroughbred horses in Japan. Cells were Gram-positive, strictly anaerobic, catalase-negative, non-spore-forming, non-motile rods that occurred in chains. They were placed in the same subcluster based on 16S rRNA gene sequence analysis, phenotypic characteristics and levels of DNA-DNA relatedness. Their DNA G + C content ranged from 36 to 38 mol%. Lactobacillus catenaformis, Lactobacillus vitulinus and Catenibacterium mitsuokai belong to cluster XVII of the Clostridium subphylum. Strain ST18(T) was most closely related to L. catenaformis ATCC 25536(T) in the phylogenetic tree, but these strains shared only 89.9 %/o (1336/1486 bp) 16S rRNA gene sequence similarity. L. catenaformis, L. vitulinus and C. mitsuokai are homofermentative bacteria, whereas ST18(T) produced CO2 from glucose. Whereas the cell-wall pepticloglycan type of L. catenaformis and L. vitulinus was L-LyS-L-Ala(3), that of C. mitsuokai and the subgroup represented by ST18(T) was A1 gamma-. (L-Ala-D-Glu-meso-diaminopimelic acid). On the basis of 16S rRNA gene sequence divergence of more than 10 % from L. catenaformis as well as phenotypic characteristics, strains ST18(T), HM244, HM250 and D149 are considered to represent a novel species of a new genus belonging to the Clostridium subphylum cluster XVII, for which the name Sharpea azabuensis gen. nov., sp. nov. is proposed. The type strain of Sharpea azabuensis is ST18(T) (=JCM 14210(T) =DSM 18934 T).

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  • Effect of oral administration of 3,3&apos;,4,4&apos;,5-pentachlorobiphyenl on the intestinal microbiota of Sprague-Dawley rats 査読

    Kaoru Yamazaki, Takehito Suzuki, Mitsuyuki Shirai, Tatsuya Takizawa, Tadashi Shinoda, Toshio Masaoka, Fumiaki Akahori, Hidetoshi Morita

    ANIMAL SCIENCE JOURNAL   79 ( 3 )   391 - 400   2008年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    The effect of the endocrine-disrupting chemical 3,3&apos;,4,4&apos;,5-pentachlorobiphyenl (PCB 126) on intestinal microbiota after oral administration, and the improvement of intestinal microbiota and feces quantity by the subsequent administration of Lactobacillus acidophilus or Lactobacillus reuteri was investigated. All the rats were given 100 mu g/kg bodyweight of PCB 126. The changes in bacterial counts were confirmed using a culture method. The administration of PCB 126 tended to decrease the bacterial counts of lactobacilli (10(9.6)-10(10.2) to 10(8.8)-10(9.2)) and bifidobacteria (10(5.3)-10(6.1) to 10(3.6)-10(4.2)), and to increase those of Enterobacteriaceae (10(8.2)-10(9.1) to 10(9.4)-10(10.3)) and staphylococci (10(6.6)-10(7.4) to 10(7.2)-10(8.4)) compared to no PCB 126 administration. After administration of PCB 126, L. acidophilus or L. reuteri orally administered to rats caused Enterobacteriaceae and staphylococci counts to decrease, suggesting that the intestinal microbiota was improved by the lactobacilli. The administration of L. acidophilus and L. reuteri improved the balance of intestinal microbiota, and defecation volume returned to its normal level. L. acidophilus and L. reuteri have a remedial effect on intestinal microbiota affected by PCB 126 and can function to lessen accumulated PCB 126 volume.

    DOI: 10.1111/j.1740-0929.2008.00542.x

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  • Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production 査読

    Hidetoshi Morita, Hidehiro Toh, Shinji Fukuda, Hiroshi Horikawa, Kenshiro Oshima, Takehito Suzuki, Masaru Murakami, Shin Hisamatsu, Yukio Kato, Tatsuya Takizawa, Hideo Fukuoka, Tetsuhiko Yoshimura, Kikuji Itoh, Daniel J. O&apos;Sullivan, Larry L. McKay, Hiroshi Ohno, Jun Kikuchi, Toshio Masaoka, Masahira Hattori

    DNA RESEARCH   15 ( 3 )   151 - 161   2008年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Lactobacillus reuteri is a heterofermentative lactic acid bacterium that naturally inhabits the gut of humans and other animals. The probiotic effects of L. reuteri have been proposed to be largely associated with the production of the broad-spectrum antimicrobial compound reuterin during anaerobic metabolism of glycerol. We determined the complete genome sequences of the reuterin-producing L. reuteri JCM 1112(T) and its closely related species Lactobacillus fermentum IFO 3956. Both are in the same phylogenetic group within the genus Lactobacillus. Comparative genome analysis revealed that L. reuteri)CM 1112(T) has a unique cluster of 58 genes for the biosynthesis of reuterin and cobalamin (vitamin B(12)). The 58-gene cluster has a lower GC content and is apparently inserted into the conserved region, suggesting that the cluster represents a genomic island acquired from an anomalous source. Two-dimensional nuclear magnetic resonance (2D-NMR) with (13)C(3)-glycerol demonstrated that L. reuteri JCM 1112(T) could convert glycerol to reuterin in vivo, substantiating the potential of L. reuteri JCM 1112(T) to produce reuterin in the intestine. Given that glycerol is shown to be naturally present in feces, the acquired ability to produce reuterin and cobalamin is an adaptive evolutionary response that likely contributes to the probiotic properties of L. reuteri.

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  • Lactobacillus equigenerosi sp nov., a coccold species isolated from faeces of thoroughbred racehorses 査読

    Akihito Endo, Stefan Roos, Eiichi Satoh, Hidetoshi Morita, Sanae Okada

    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY   58 ( Pt 4 )   914 - 918   2008年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MICROBIOLOGY SOC  

    Two strains of lactic acid bacteria were isolated from faeces of two actively racing thoroughbred horses. The isolates formed a subcluster in the Lactobacillus reuteri phylogenetic group, closely related to Lactobacillus fermentum, L. gastricus, L. ingluviei and L. mucosae, by phylogenetic analysis based on 16S rRNA gene sequences. Levels of DNA-DNA relatedness revealed that the isolates belonged to the same taxon and were genetically separated from their phylogenetic relatives. Biochemical and physiological characteristics also distinguished the isolates from their phylogenetic relatives. The isolates produced spherical or oval cells, and tetrad-like cells were rarely seen. To the best of our knowledge, this is the first report of this morphological characteristic within the genus Lactobacillus. Thus, the isolates represent an atypical novel species of the genus Lactobacillus, for which the name Lactobacillus equigenerosi sp. nov. is proposed. The type strain is NRIC 0697(T) (=JCM 14505(T) =DSM 18793(T)).

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  • Aqueous extracts of Rhizopus oryzae induce apoptosis in human promyelocytic leukemia cell line HL-60 査読

    Takehito Suzuki, Setsuo Ushikoshi, Hidetoshi Morita, Hideo Fukuoka

    JOURNAL OF HEALTH SCIENCE   53 ( 6 )   760 - 765   2007年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PHARMACEUTICAL SOC JAPAN  

    Protection against in vivo infection with Salmonella and enhancement of in vitro superoxide production by peripheral blood neutrophils are two reported effects of treatment with aqueous extracts of the fungus Rhizopus oryzae U-1 (Aq-ROU). Here, we report that Aq-ROU also has antiproliferative activity and can induce apoptosis in a human promyelocytic leukemia cell line, HL-60. During Aq-ROU-induced apoptosis, HL-60 cells undergo genomic DNA fragmentation characteristic of apoptosis after just 6 hr of treatment. Using phosphatidylserine (PS) as an indicator of apoptosis, we also found that the proportion of apoptotic cells increased significantly after 9 hr of treatment. Indeed, induction of apoptosis by Aq-ROU reached 43.3% after 24 hr of treatment, which is comparable to the effects of a known apoptosis-inducer, actinomycin D. Moreover, the activities of caspase-3, -8, and -9 increased in parallel with Aq-ROU treatment, with a peak of activity 9 hr after the initial treatment. Taken together, the results suggest that R. oryzae contains one or more water-soluble factor that can reliably and efficiently induce apoptosis in human cells via activation of caspase-3.

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  • Lactobacillus hayakitensis sp nov, isolated from intestines of healthy thoroughbreds 査読

    Hidetoshi Morita, Chiharu Shiratori, Masaru Murakami, Hideto Takami, Yukio Kato, Akihito Endo, Fumihiko Nakajima, Misako Takagi, Hiroaki Akita, Sanae Okada, Toshio Masaoka

    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY   57 ( Pt 12 )   2836 - 2839   2007年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Two strains, KBL13(T) and GBL13, were isolated as one of intestinal lactobacilli from the faecal specimens from different thoroughbreds of the same farm where they were born in Hokkaido, Japan. They were Gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming and non-motile rods. KBL13(T) and GBL13 homofermentatively metabolize glucose, and produce lactate as the sole final product from glucose. The 16S rRNA gene sequence, DNA-DNA hybridization, DNA G + C content and biochemical characterization indicated that these two strains, KBL13(T) and GBL13, belong to the same species. In the representative strain, KBL13(T) the DNA G + C content was 34.3 mol%. Lactobacillus salivarius JCM 1231(T) (=ATCC 11741(T); AF089108) is the type strain most closely related to the strain KBL13(T) as shown in the phylogenetic tree, and the 16S rRNA gene sequence identity showed 96.0% (1425/1484 bp). Comparative 16S rRNA gene sequence analysis of this strain indicated that the two isolated strains belong to the genus Lactobacillus and that they formed a branch distinct from their closest relatives, L. salivarius, Lactobacillus aviarius, Lactobacillus saerimneri and Lactobacillus acidipiscis. DNA-DNA reassociation experiments with L. salivarius and L. aviarius confirmed that KBL13(T) represents a novel species, for which the name Lactobacillus hayakitensis sp. nov. is proposed. The type strain is KBL13T (=jCM 14209(T) =DSM 18933(T)).

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  • Toll-like receptor (TLR) 2 induced through TLR4 signaling initiated by Helicobacter pylori cooperatively amplifies iNOS induction in gastric epithelial cells 査読

    Kaname Uno, Katsuaki Kato, Tomoaki Atsumi, Takehito Suzuki, Jun Yoshitake, Hidetoshi Morita, Shuichi Ohara, Yashige Kotake, Tooru Shimosegawa, Tetsuhiko Yoshimura

    AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY   293 ( 5 )   G1004 - G1012   2007年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Cell- surface Toll- like receptors ( TLRs) initiate innate immune responses, such as inducible nitric oxide synthase ( iNOS) induction, to microorganisms' surface pathogens. TLR2 and TLR4 play important roles in gastric mucosa infected with Helicobacter pylori ( H. pylori), which contains lipopolysaccharide ( LPS) as a pathogen. The present study investigates their physiological roles in the innate immune response of gastric epithelial cells to H. pylori- LPS. Changes in the expression of iNOS, TLR2, and TLR4, as well as downstream activation of mitogen-activated protein kinases and nuclear factor-kappa B (NF-kappa B), were analyzed in normal mouse gastric mucosal GSM06 cells following stimulation with H. pylori-LPS and interferon-gamma. Specific inhibitors for mitogen-activated protein kinases, NF-kappa B, and small interfering RNA for TLR2 or TLR4 were employed. The immunohistochemistry of TLR2 was examined in human gastric mucosa. H. pylori- LPS stimulation induced TLR2 in GSM06 cells, but TLR4 was unchanged. TLR2 induction resulted from TLR4 signaling that propagated through extracellular signal- related kinase and NF-kappa B activation, as corroborated by the decline in TLR4 expression on small interfering RNA treatment and pretreatment with inhibitors. The induction of iNOS and the associated nitric oxide production in response to H. pylori- LPS stimulation were inhibited by declines in not only TLR4 but also TLR2. Increased expression of TLR2 was identified in H. pylori- infected human gastric mucosa. TLR4 signaling initiated by H. pylori- LPS and propagated via extracellular signal- regulated kinase and NF-kappa B activation induced TLR2 expression in gastric epithelial cells. Induced TLR2 cooperated with TLR4 to amplify iNOS induction. This positive correlation may constitute a mechanism for stimulating the innate immune response against various bacterial pathogens, including H. pylori- LPS.

    DOI: 10.1152/ajpgi.00096.2007

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  • Potency of water extracts of Rhizopus oryzae on the Salmonella infection rat 査読

    Takehito Suzuki, Hideo Fukuoka, Setsuo Ushikoshi, Hidetoshi Morita

    FOOD SCIENCE AND TECHNOLOGY RESEARCH   13 ( 3 )   235 - 240   2007年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    Rhizopus oryzae U-1 water extract (ROU-we) was administered orally to rats at 10 mg/kg body weight for 9 days. Salmonella enteritidis was inoculated at a dose of 10(9)CFU/animal. The following protective effects of ROU-we against infection were examined: cell counts of Salmonella in organs of infected rats (liver, spleen, and mesenteric lymph nodes); phagocytic capacity of collected peripheral monocytes and peritoneal macrophages; cell counts of respective helper T-cell subclasses (Th0, Th1, and Th2); and the leukocyte percentage in peripheral blood. The Salmonella cell count in the liver of the ROU-we group decreased significantly compared to that of the control group and the peripheral monocytes' phagocytic capacity increased 4.5-fold. Moreover, the ROU-we group's Th1 response was higher than the infected control group. However, the healthy control group and the ROU-we group showed similar Th1/Th2 balance and cell count tendencies. These results suggest that ROU-we activated peripheral monocytes and improved Th1/Th2 balance, thereby strengthening immunity against Salmonella infection.

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  • Comparative metagenomics revealed commonly enriched gene sets in human gut microbiomes 査読

    Ken Kurokawa, Takehiko Itoh, Tomomi Kuwahara, Kenshiro Oshima, Hidehiro Toh, Atsushi Toyoda, Hideto Takami, Hidetoshi Morita, Vineet K. Sharma, Tulika P. Srivastava, Todd D. Taylor, Hideki Noguchi, Hiroshi Mori, Yoshitoshi Ogura, Dusko S. Ehrlich, Kikuji Itoh, Toshihisa Takagi, Yoshiyuki Sakaki, Tetsuya Hayashi, Masahira Hattori

    DNA RESEARCH   14 ( 4 )   169 - 181   2007年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Numerous microbes inhabit the human intestine, many of which are uncharacterized or uncultivable. They form a complex microbial community that deeply affects human physiology. To identify the genomic features common to all human gut microbiomes as well as those variable among them, we performed a large-scale comparative metagenomic analysis of fecal samples from 13 healthy individuals of various ages, including unweaned infants. We found that, while the gut microbiota from unweaned infants were simple and showed a high inter-individual variation in taxonomic and gene composition, those from adults and weaned children were more complex but showed a high functional uniformity regardless of age or sex. In searching for the genes over-represented in gut microbiomes, we identified 237 gene families commonly enriched in adult-type and 136 families in infant-type microbiomes, with a small overlap. An analysis of their predicted functions revealed various strategies employed by each type of microbiota to adapt to its intestinal environment, suggesting that these gene sets encode the core functions of adult and infant-type gut microbiota. By analysing the orphan genes, 647 new gene families were identified to be exclusively present in human intestinal microbiomes. In addition, we discovered a conjugative transposon family explosively amplified in human gut microbiomes, which strongly suggests that the intestine is a 'hot spot' for horizontal gene transfer between microbes.

    DOI: 10.1093/dnares/dsm018

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  • Molecular profiling of Lactobacillus, Streptococcus, and Bifidobacterium species in feces of active racehorses 査読

    Akihito Endo, Sanae Okada, Hidetoshi Morita

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   53 ( 3 )   191 - 200   2007年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MICROBIOL RES FOUNDATION  

    Diversity and compositions of the Lactobacillus, Streptococcus, and Bifidobacterium group in the feces of six healthy, actively racing horses (Thoroughbreds) were analyzed by using PCR-denaturing gradient gel electrophoresis (DGGE) and real-time PCR with primer sets specific for each group. PCR-DGGE analysis of the feces showed that Lactobacillus equi, Lactobacillus johnsonii, a phylogenetic relative of Lactobacillus salivarius, a phylogenetic relative of Lactobacillus gastricus, and Weissella confusa were predominant in almost all of the feces tested, and Streptococcus bovis/Streptococcus equinus was predominant in the Streptococcus group. The Bifidobacterium group was not detected by single-PCR but atypical species of the group were found in three of the six Thoroughbreds tested by nested-PCR. Calculation and estimation of lactic acid bacteria and bifidobacteria revealed that lactic acid bacteria were predominant in the feces and bifidobacteria were minor. These results indicate that the community of lactic acid bacteria and bifidobacteria in horse feces are unique because of the presence of specific species for horse feces and a minority of the Bifidobacterium group. Repeated tests of the feces from the same horse over 3 months showed that the diversity and composition of lactic acid bacteria and bifidobacteria in the feces was basically stable throughout the test period.

    DOI: 10.2323/jgam.53.191

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  • Molecular monitoring of the main changes in bacterial floral diversity in the gastrointestinal tract of a thoroughbred foal with catarrhal enteritis by using PCR-DGGE 査読

    Hidetoshi Morita, Fumihiko Nakajima, Masaru Murakami, Akihito Endo, Takehito Suzuki, Chiharu Shiratori, Yukio Kato, Alexandre T. Okatani, Hiroaki Akita, Toshio Masaoka

    JOURNAL OF EQUINE VETERINARY SCIENCE   27 ( 1 )   14 - 19   2007年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    In this study, the main changes in bacterial floral diversity in the gastrointestinal tract of a Thoroughbred foal were monitored by using polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). The foal died of catarrhal enteritis of the cecum and large colon. Diarrheal feces and gastrointestinal contents were compared with normal feces. The closest relatives of the bacterium in the samples were Lactobacillus johnsonii (100% similarity), uncultured Bacteroides sp. (92.5% similarity), Bacteroides fragilis (96.3% similarity), and Enterococcus faecium/Enterococcus durans (100% similarity); these were detected by PCR-DGGE using a universal primer set. Monitoring revealed that the numbers of Escherichia coli/Shigella sonnei (97.9% similarity) were significantly higher in the diarrheal feces. Thus, PCR-DGGE is a useful tool for monitoring the main changes in bacterial floral diversity occurring in the gastrointestinal tracts of Thoroughbreds.

    DOI: 10.1016/j.jevs.2006.11.011

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  • An improved DNA isolation method for metagenomic analysis of the microbial flora of the human intestine 査読

    Hidetoshi Morita, Tomomi Kuwahara, Kenshiro Ohshima, Hiroyuki Sasamoto, Kikuji Itoh, Masahira Hattori, Tetsuya Hayashi, Hideto Takami

    MICROBES AND ENVIRONMENTS   22 ( 3 )   214 - 222   2007年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE  

    The efficiency with which lysis of five strictly anaerobic and six facultatively anaerobic bacterial species, all well-known human colonic commensals, were lysed was tested using a reference method for general metagenomic analysis and an improved method that involves higher levels of lysozyme and proteinase K, as well as the addition of achromopeptidase. Ten species were lysed with an efficiency of &gt;80% by the reference method, while the lytic efficiency for Clostridium ramosum JCM 1298(T) was &lt;50%. The lytic efficiency of the improved method for C. ramosum JCM 1298(T) was 82.5%. Similarly, five samples of human feces were tested with these methods, as well as with the QIAamp DNA stool mini kit. Although the efficiency of lysis of the microbes recovered from the fecal samples fluctuated depending on the sample in the cases of the reference method (13.384.6%) and QIAamp DNA stool mini kit (38.8-69.2%), the improved method gave stable and high-level lysis (&gt;90%) for all the fecal samples. Accordingly, since the DNA samples isolated by the improved method can reflect nearly true genomic information in the microbial flora, our improved method should be applicable to metagenomic analyses, not only for bacteria in the human intestine but also for bacteria in other environments.

    DOI: 10.1264/jsme2.22.214

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  • The formation of g=2.49-species of cytochrome P450 in the rat liver by PCB126 oral administration: Identification of heme axial ligands by EPR spectroscopy 査読

    Hidetoshi Morita, Hiroshi Yoshikawa, Tatsuya Takizawa, Mitsuyuki Shirai, Furniaki Akahori, Tetsuhiko Yoshimura

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   70 ( 12 )   2974 - 2981   2006年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Rat livers and microsomes were subjected to electron paramagnetic resonance (EPR) measurements at 77K. The EPR spectra of the livers from the control group, carbon tetrachloride-, 3-methylcholanthrene-, and 3,3',4,4',5-pentachlorobiphenyl (PCB126)-treated rats exhibited an EPR spectrum at g = 2.40, 2.24, and 1.93, which is characteristic of P450 in a resting state. The liver of the PCB126-treated rats showed an additional distinct EPR spectrum at g = 2.49, 2.26, and 1.87 (g = 2.49-species). The heme environmental structure of g = 2.49-species was identified by crystal field analysis using three EPR g-values of the microsome treated with various chemicals. These results indicated that g = 2.49-species is a hemeprotein with cysteine thiolate at the 5th coordination site, and a nitrogenous ligand at the 6th site.

    DOI: 10.1271/bbb.60367

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  • Paenibacillus macerans possesses two types of 16S rDNA copies in a genome with a length difference of twelve base pairs 査読

    Y Hamasaki, Y Watanabe, S Kotoura, H Fuchu, M Sugiyama, K Hashizume, H Morita

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   69 ( 10 )   1995 - 1998   2005年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Two Paenibacillus macerans strains, JCM 2500(T) and MCRI 12, exhibited two types of 16S rDNA copies in their genomes, accompanied by a length difference of 12bp at positions 203 to 214 (Escherichia coli numbering). The long-type sequences were newly identified for P. macerans 16S rDNA, and the copy numbers were different between the two strains. Both types of 16S rRNA were expressed in each strain, and it was predicted that the polymorphism at this position is located in helix H10, based on a comparison with the E. coli 16S rRNA secondary structure model.

    DOI: 10.1271/bbb.69.1995

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  • Anti-microbial action against verotoxigenic Escherichia coli O157 : H7 of nitric oxide derived from sodium nitrite 査読

    H Morita, H Yoshikawa, T Suzuki, S Hisamatsu, Y Kato, R Sakata, Y Nagata, T Yoshimura

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   68 ( 5 )   1027 - 1034   2004年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    The levels of verotoxin-1 and verotoxin-2 released by verotoxigenic Escherichia coli O157:117 treated in vitro with sodium nitrite, sodium chloride and several antibiotics were evaluated. Of the three strains of E. coli O157:117 used in this study, two strains produced both verotoxin-1 and verotoxin-2, and one strain produced only verotoxin-2. Treatment of E. coli O157:H7 with sodium nitrite (6,000 mg/l, minimum inhibitory concentration) did not increase the levels of verotoxin-1 and verotoxin-2 compared with a treatment by sodium chloride or antibiotics. When the electron paramagnetic resonance spectrum of sodium nitrite-treated bacterial cells was examined at 77 K to clarify the mechanism for the anti-bacterial activity of nitric oxide derived from sodium nitrite, electron paramagnetic resonance signals with g-values of 2.035 and 2.010 were observed. These were identified as being derived from iron-nitric oxide complexes. It appears that the dinitrosyl iron complexes in the E. coli O157:117 cells were generated from the reaction of iron-sulfur proteins (enzymes) with nitric oxide formed by the reduction of sodium nitrite. The amount of ATP was decreased by the presence of sodium nitrite in the cell suspension. These findings indicate that nitric oxide derived from sodium nitrite penetrated the cells and inactivated enzymes related to the respiratory chain.

    DOI: 10.1271/bbb.68.1027

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  • Behavior of psychrotrophic lactic acid bacteria isolated from spoiling cooked meat products 査読

    Y Hamasaki, M Ayaki, H Fuchu, M Sugiyama, H Morita

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   69 ( 6 )   3668 - 3671   2003年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Three kinds of lactic acid bacteria were isolated from spoiling cooked meat products stored below 10degreesC. They were identified as Leuconostoc mesenteroides subsp. mesenteroides, Lactococcus lactis subsp. lactis, and Leuconostoc citreum. All three strains grew well in MRS broth at 10degreesC. In particular, L. mesenteroides subsp. mesenteroides and L. citreum grew even at 4degreesC, and their doubling times were 23.6 and 51.5 h, respectively. On the other hand, although the bacteria were initially below the detection limit (&lt;10 CFU/g) in model cooked meat products, the bacterial counts increased to 10(8) CFU/g at 10degreesC after 7 to 12 days.

    DOI: 10.1128/AEM.69.6.3668-3671.2003

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  • Expression of nitric oxide synthase isoforms and detection of nitric oxide in rat placenta 査読

    T Takizawa, H Yoshikawa, M Yamada, H Morita

    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY   282 ( 4 )   C762 - C767   2002年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Nitric oxide (NO) production in the rat placenta was monitored and quantified by electron paramagnetic resonance (EPR) spectroscopy with hemoglobin and an Fe-N-(dithiocarboxy) sarcosine (DTCS) complex as NO-trapping reagents. Expression of nitric oxide synthase (NOS) isoforms was also examined by quantitative RT-PCR analysis. The EPR spectrum of the placenta with hemoglobin trapping showed a three-line hyperfine structure (g = 2.008 and a = 1.66-mT). The EPR signal was diminished after the placenta was homogenized or the NOS inhibitor L-NAME was administered to pregnant rats. Therefore, the specific signal was definitely identified as being derived from endogenous NO spin-trapped by hemoglobin, and the EPR spectrum showed that the NO adduct existed as a pentacoordinate alpha-NO heme species. The EPR spectrum of the placenta with Fe-DTCS trapping showed a triplet signal (g = 2.038) derived from an NO-Fe-DTCS complex. The height of the triplet signal did not vary significantly with gestational stage during the last few days of gestation. At the gestational stages examined, the level of NOS II mRNA expression was significantly higher than that of NOS III mRNA. NOS II expression in term (day 21.5) placenta was significantly increased compared with that in preterm (day 19.5) placenta (P &lt; 0.01, n = 4 or 5). These results suggest that NOS II is the predominant producer of NO in the placenta and that NOS II-generated NO plays significant roles in the maintenance of placental functions immediately before birth.

    DOI: 10.1152/ajpcell.00101.2001

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  • Physico-chemical and processing quality of porcine M. longissimus dorsi frozen at different temperatures 査読

    R. Sakata, T. Oshida, H. Morita, Y. Nagata

    Meat Science   39 ( 2 )   277 - 284   1995年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Longissimus dorsi muscle from six pigs (24 h post-mortem) was cut into portions of similar size and shape (c. 700 g) and vacuum-packed in polyfilm. The muscle specimens were divided into three samples, one frozen at -20°C, another at -80°C and the third served as the control (not frozen). The meat sample frozen at -80°C was transferred to the -20°C freezer. After one month, both frozen pork samples were thawed at -2°C and drip loss (%) was measured. Hunter colour, metmyoglobin (MetMb) formation (%), water-holding capacity (WHC), TBA value, transmission value (TM) and myofibril fragmentation were also determined. There was no significant difference in drip loss for the two frozen samples. No MetMb formation could be detected and Hunter values were basically the same for all three samples. WHC, TBA value and TM were essentially the same for all three samples. TBA value was quite low for each frozen sample, indicating that lipid oxidation did not occur during freezing. Histological examination of both frozen samples indicated inter- and intracellular ice crystal formation at -20°C, and intracellular ice at -80°C, the extent being less than at -20°C. At -20°C, ice crystals were larger and muscle fibre diameter smaller than for the control or -80°C sample. Myofibril fragmentation in both frozen samples was significantly higher than in the control. Pork sausage was prepared from all three samples by adding 2% NaCl and 100 ppm NaNO2. Cooking loss and colour forming ratios were essentially the same. The sausage sample made from the -20°C frozen meat was harder than that of the other two samples according to rheological measurement. © 1994.

    DOI: 10.1016/0309-1740(94)P1828-J

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  • Intergeneric transfer of lactose-fermenting ability to Pediococcus acidilactici by conjugation 査読

    Hidetoshi Morita, Taku Miyamoto, Kei Kataoka

    Agricultural and Biological Chemistry   55 ( 11 )   2871 - 2873   1991年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/00021369.1991.10857756

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▼全件表示

書籍等出版物

  • 食品免疫学事典

    日本食品免疫学会 編( 担当: 分担執筆 ,  範囲: p.218-219, Proteobacteria; p.220-221, Verrucomicrobia)

    朝倉書店  2021年11月 

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  • 医薬品開発におけるオミックス解析技術~ゲノム・トランスクリプトーム・プロテオーム・メタボローム~

    森田 英利( 担当: 共著 ,  範囲: p.174-p.181 第4節 網羅的な細菌叢解析から解明されてきたヒト腸内細菌叢のもつ生体への影響)

    株式会社情報機構  2020年3月 

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  • Alzheimer's Disease

    Fujii Y, Khanobish A, Morita H( 担当: 共著 ,  範囲: Relationship between Alzheimer's disease and human microbiome)

    Codon Publications  2019年11月 

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    総ページ数:12   担当ページ:Chapter 9 p.149-160   記述言語:英語 著書種別:学術書

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  • 共生微生物―生物と密接に関わるミクロな生命体

    森田 英利( 担当: 共著 ,  範囲: プロバイオティクス研究とその歴史)

    化学同人  2016年 

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    総ページ数:13   担当ページ:45-57   記述言語:日本語 著書種別:学術書

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  • わかりやすい食品機能学

    森田 英利( 担当: 共著 ,  範囲: 2-4 腸内環境を整える成分, 2-7 抗疲労効果成分, 5-1 機能性食品の制度, 5-2 バイオテクノロジーと機能性食品, 5-3未来の機能性食品-テーラーメイド食設計の時代に向けて-)

    三共出版  2014年 

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  • 微生物学 第2版(新バイオテクノロジーテキストシリーズ)

    森田 英利( 担当: 監修 ,  範囲: 第3章 微生物の代謝, 第4章 微生物の増殖と分化, 第8章 環境における微生物の生態系)

    NPO法人日本バイオ技術教育学会  2013年 

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  • 微生物機能学 -微生物リソースと遺伝子リソースの応用-

    森田 英利( 担当: 編集 ,  範囲: 10. 二酸化炭素から有機化合物を合成する -微生物による炭素固定, 14. 利用者にとって有用な遺伝子組換え微生物を作出する -遺伝子組換え体の利用)

    三共出版  2012年 

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  • Handbook of Molecular Microbial Ecology II: Metagenomics in Different Habitats

    MORITA Hidetoshi( 担当: 共著 ,  範囲: Chapter 19. Assessment and Improvement of Methods for Microbial DNA Preparation from Fecal Samples)

    John Wiley & Sons, Inc.  2011年 

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    記述言語:英語 著書種別:学術書

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  • 動物応用科学の展開 ―人と動物の共生を目指して―

    森田 英利( 担当: 共著 ,  範囲: 乳酸菌・ビフィズス菌のヒトと動物の健康への貢献とゲノム解析情報の応用)

    養賢堂  2011年 

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  • 乳酸菌とビフィズス菌のサイエンス

    森田 英利( 担当: 共編者(共編著者) ,  範囲: 第3章 1乳酸菌・ビフィズス菌ゲノムの基本情報, 第4章 3 ロイテリン, 第8章 4 メタゲノム解析とその応用)

    京都大学学術出版会  2010年 

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  • メタゲノム解析技術の最前線

    森田 英利( 担当: 共著 ,  範囲: 第2章 5. 腸内細菌叢ゲノムDNAの調製法, 林 潤一郎, 第4章 5. 口腔内フローラのメタゲノム解析)

    シーエムシー出版  2010年 

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  • 乳酸菌の保健機能と応用

    森田 英利( 担当: 共著 ,  範囲: 第9章 乳酸菌・ビフィズス菌のゲノミクス・プロテオミクス)

    シーエムシー出版  2007年 

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  • わかりやすい食物と健康①―食品の分類と特性―

    森田 英利( 担当: 共著 ,  範囲: 3-3. 動物性食品 卵類, 3-4. 森田英利, 動物性食品 乳類)

    三共出版  2007年 

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  • プロバイオティクスとバイオジェニクス:科学的根拠と今後の開発展望

    森田 英利( 担当: 共編者(共編著者) ,  範囲: 第4章 プロバイオティクスとバイオジェニクスを理解するための先端科学, 第1節 2. 乳酸菌)

    エヌ・ティー・エス  2005年 

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  • 管理栄養士国家試験のための用語辞典

    森田 英利( 担当: 共著 ,  範囲: 14項目の語句解説を担当)

    海馬書房  2005年 

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  • 色から見た食品のサイエンス

    森田 英利( 担当: 共著 ,  範囲: 第8節 動物由来の色素)

    サイエンスフォーラム  2004年 

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  • Meat in Science and Industry

    Hidetoshi Morita( 担当: 共著 ,  範囲: Two coordinate States of Nitrosyl Myoglobin and Structural Change by pH)

    University Education Press  2002年 

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    記述言語:英語 著書種別:教科書・概説・概論

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  • ゲノミクス・プロテオミクスの新展開~生物情報の解析と応用~

    森田 英利( 担当: 共著 ,  範囲: 第4編 第1章 第7節 乳酸菌のゲノム解析について)

    エヌ・ティー・エス  2002年 

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  • 畜産食品の事典

    森田 英利( 担当: 共著 ,  範囲: 24. 食肉の色)

    朝倉書店  2001年 

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  • 生体内一酸化窒素(NO)実験プロトコール

    森田 英利( 担当: 共著 ,  範囲: 第23章 細菌のNO分析)

    共立出版  2000年 

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  • 食品学各論

    森田 英利( 担当: 共著 ,  範囲: 3-3. 動物性食品 卵類, 3-4. 森田英利, 動物性食品 乳類)

    三共出版  1999年 

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  • 乳酸菌の科学と技術

    森田 英利( 担当: 共著 ,  範囲: 第3章 第2節 糖の輸送系とカタボライト制御, 第5章 第1節 ラクトース発酵性, 第5章 第3節 フレーバー生産性)

    学会出版センター  1996年 

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  • ヒト腸内細菌叢とアルツハイマー病疾患 招待

    藤井 祐介, 森田 英利

    メディカル・サイエンス・ダイジェスト   45 ( 12 )   48 - 50   2019年10月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • 腸内細菌が産生するD型アミノ酸の新たな腎臓保護効果を発見~腎臓と腸管・腸内細菌叢をつなぐ新たな全身ネットワーク~

    Nakade Y, Iwata Y, Furuichi K, Mita M, Hamase K, Konno R, Miyake T, Sakai N, Kitajima S, Toyama T, Shinozaki Y, Sagara A, Miyagawa T, Hara A, Shimizu M, Kamikawa Y, Sato K, Oshima M, Yoneda-Nakagawa S, Yamamura Y, Kaneko S, Miyamoto T, Katane M, Homma H, Morita H, Suda W, Hattori M, Wada T

    Kanazawa University News Release   2018年11月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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  • アルツハイマー病の背景と発症 ―ヒトマイクロバイオーム(細菌叢)との関連の視点から― 査読

    藤井 祐介, 森田 英利

    岡山大学農学部学術報告   107   5 - 10   2018年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • 同種造血幹細胞移植前における腸内細菌叢の多様性と移植成績の検討

    黒澤修兵, 土岐典子, 隅山昌洋, 隅山昌洋, 笹島哲史, 五十嵐愛子, 三村伊予, 森田英利, 藤岡優樹, 藤岡優樹, 西川博嘉, 西川博嘉, 島津裕, 須田亙, 須田亙, 服部正平, 服部正平, 名島悠峰, 垣花和彦, 坂巻壽, 本田賢也, 本田賢也, 大橋一輝

    日本造血細胞移植学会総会プログラム・抄録集   39th   221   2017年2月

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  • プロバイオティクス製品投与後の体内挙動と作用 招待 査読

    森田 英利, 藤井 祐介

    薬局   68 ( 11 )   3370 - 3380   2017年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • アルツハイマー病に関する腸内細菌叢の視点からの研究

    藤井 祐介, 森田 英利

    Bio Clinica   2017年 ( 8月号 )   69 - 74   2017年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • ヒトマイクロバイオーム研究から俯瞰するアルツハイマー病について 招待 査読

    森田 英利

    BIO Clinica   2017年 ( 12月臨時増刊号 )   2017年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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  • Clinical Impact of Pre-Transplant Microbial Diversity on Transplant Outcomes

    Shuhei Kurosawa, Noriko Doki, Masahiro Suyama, Satoshi Sasajima, Aiko Igarashi, Iyo Mimura, Hidetoshi Morita, Yuki Fujioka, Hiroyoshi Nishikawa, Yutaka Shimazu, Wataru Suda, Masahira Hattori, Yuho Najima, Kazuhiko Kakihana, Hisashi Sakamaki, Kenya Honda, Kazuteru Ohashi

    BLOOD   128 ( 22 )   2016年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER SOC HEMATOLOGY  

    Web of Science

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  • モンゴルの伝統的発酵乳である馬乳酒“アイラグ”から分離された抗菌物質を産生するLeuconostoc mesenteroidesの比較ゲノム解析

    羽野知紘, 藤英博, 大島健志朗, BOLORMAA Tsognemekh, BURENJARGAL Sedkhuu, WULIJIDELIGEN, 中野章代, 吉田光希, NGUYEN Co T. K, NGUYEN Tien T. T, 三村伊予, 荒川健佑, 田代康介, 宮本拓, 宮本拓, 森田英利, 森田英利

    日本乳酸菌学会誌   27 ( 2 )   141   2016年6月

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    記述言語:日本語  

    J-GLOBAL

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  • 家畜の生産性を向上させる新規プロバイオティクス細菌,Bacillus hisashii N‐11<sup>T</sup>

    渡邉凌, 西田彩夏, 宮本浩邦, 宮本浩邦, 宮本浩邦, 宮本浩邦, 堀内三吉, 森田英利, 福田真嗣, 福田真嗣, 大野博司, 大野博司, 大野博司, 市野瀬志津子, 宮本久, 児玉浩明

    日本農芸化学会大会講演要旨集(Web)   2016   3F002 (WEB ONLY)   2016年3月

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  • 比較メタゲノム解析による日本人腸内細菌叢の特徴解明

    西嶋傑, 須田亙, 須田亙, 大島健志朗, 金錫元, 広瀬侑, 飯岡絵里香, 進藤智絵, 山下直子, 黒川李奈, 森田英利, 服部正平, 服部正平

    日本ゲノム微生物学会年会要旨集   10th   58   2016年

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    記述言語:日本語  

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  • ヒト腸内細菌叢メタゲノムデータの国間比較解析

    西嶋傑, 須田亙, 飯岡恵里香, 木内美沙, 進藤智絵, 山下直子, 小宮恵子, 金錫元, 大島健志朗, 森田英利, 服部正平

    腸内細菌学雑誌   29 ( 2 )   96   2015年4月

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    記述言語:日本語  

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  • ヒト腸内細菌叢の国特異的構造の発見とその形成要因の解明

    西嶋傑, 大島健志朗, 金錫元, 須田亙, 飯岡恵里香, 木内美沙, 進藤智絵, 山下直子, 小宮恵子, 森田英利, 服部正平

    日本ゲノム微生物学会年会要旨集   9th   57   2015年

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  • ハダカデバネズミの腸内細菌叢解析とハダカデバネズミ(クイーン)腸内細菌叢によるノトバイオートマウスでの生体影響

    森田英利, 竹尾淳, 須田亙, 度会晃行, 高畑宗明, 大島健志朗, 三浦恭子, 新井奈月, 岡野栄之, 菊水健史, 服部正平

    腸内細菌学雑誌   28 ( 2 )   97   2014年4月

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    記述言語:日本語  

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  • アルコール依存症患者の糞便中のアセトアルデヒド代謝と網羅的な腸内細菌叢解析

    中山亨, 桑原明香, 斉藤祐太, 下山武文, 須田亙, 大島健志朗, 服部正平, 森田英利, 横山顕, 水上健, 堤絵梨, 諏訪芳秀

    日本農芸化学会大会講演要旨集(Web)   2014   4A11A09 (WEB ONLY)   2014年3月

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    記述言語:日本語  

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  • 日本人と外国人間における腸内マイクロバイオームの大規模比較解析

    西嶋傑, 大島健志朗, 金錫元, 飯岡恵里香, 大森恵美, 木内美沙, 黒柳寛実, 小宮惠子, 須田亙, 梅崎昌裕, 森田英利, 服部正平

    日本ゲノム微生物学会年会要旨集   8th   70   2014年

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    記述言語:日本語  

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  • 異なる人種間における腸内マイクロバイオームの大規模比較解析

    西嶋傑, 大島健志朗, 金錫元, 須田亙, 飯岡恵里香, 木内美沙, 黒柳寛実, 進藤智絵, 高山由紀子, 山下直子, 森田英利, 服部正平

    日本分子生物学会年会プログラム・要旨集(Web)   37th   1P-0095 (WEB ONLY)   2014年

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  • 腸内細菌叢解析によるオランウータンの個体識別法の開発

    森田英利, 須田亙, 鈴木晃, 鈴木南水子, 飯岡恵里香, 大森恵美, 高山由紀子, 進藤智絵, 服部恭江, 中野章代, 金錫元, 大島健志朗, 服部正平

    日本畜産学会大会講演要旨   117th   64   2013年9月

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    記述言語:日本語  

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  • メタゲノム解析による日本人腸内マイクロバイオームの特徴解明

    西嶋傑, 大島健志朗, 飯岡恵里香, 大森恵美, 木内美沙, 金錫元, 須田亙, 森田英利, 服部正平

    日本ゲノム微生物学会年会要旨集   7th   70   2013年

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    記述言語:日本語  

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  • 合同シンポジウム3-2&emsp;&emsp;腸内細菌によるT細胞への影響

    本田 賢也, 新 幸二, 田ノ上 大, 永野 勇治, 成島 聖子, 大島 健志朗, 須田 亙, 森田 英利, 服部 正平

    日本臨床免疫学会会誌   36 ( 5 )   315 - 315   2013年

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    記述言語:日本語   出版者・発行元:日本臨床免疫学会  

    &emsp;腸内細菌と常に接する消化管粘膜は,非常にユニークな免疫システムを形成している.中でも,Th17細胞やTreg細胞が,消化管粘膜に多数存在することが知られている.我々は無菌マウスを検討することで,Th17細胞・Treg細胞それぞれの分化・機能に腸内細菌が深く影響を与えていることを見出した.さらに腸内細菌をスクリーニングし,Th17細胞分化を誘導するマウス腸内細菌としてセグメント細菌を同定した.さらに,Treg細胞分化を誘導する細菌として,クロストリジアに属するマウス消化果敢に由来する細菌種を,同定した.更にヒト便からスタートして幾つかの選択過程を経て,Treg細胞を誘導するヒト由来クロストリジアに属する細菌株も単離した.我々の研究結果は,慢性炎症性腸疾患や,多発性硬化症などの自己免疫疾患治療に応用できる可能性があると考えている.<br>

    DOI: 10.2177/jsci.36.315

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  • 次世代シークエンサーを用いた常在細菌叢解析 (特集 体内の細菌が作るもう一つの世界 マイクロバイオームの驚異)

    須田 亙, 大島 健志朗, 森田 英利

    細胞工学   32 ( 11 )   1115 - 1119   2013年

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    記述言語:日本語   出版者・発行元:学研メディカル秀潤社 ; 1982-  

    CiNii Article

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  • 高速シークエンサー454を用いた改良16Sアンプリコンシークエンシングによる細菌叢の定量的解析

    須田亙, 飯岡恵里香, 大島健志朗, 大森恵美, 木内美沙, 金相完, 金錫元, 黒柳寛実, 小宮恵子, 進藤智絵, 高山由紀子, 服部恭江, HEBA Said, 森田英利, 服部正平

    日本分子生物学会年会プログラム・要旨集(Web)   36th   1P-0020 (WEB ONLY)   2013年

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  • 日本人と外国人間における腸内マイクロバイオームの大規模比較解析

    西嶋傑, 大島健志朗, 金錫元, 飯岡恵里香, 大森恵美, 木内美沙, 黒柳寛実, 小宮惠子, 須田亙, 森田英利, 服部正平

    日本分子生物学会年会プログラム・要旨集(Web)   36th   3P-0036 (WEB ONLY)   2013年

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    記述言語:日本語  

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  • Lactobacillus属とBifidobacterium属における線毛および鞭毛の透過型電子顕微鏡(TEM)観察

    森田英利, SRIVASTAVA Tulika, 中野章代, 高畑宗明, 高木孝士, 西山英利, 藤英博, 大島健志朗, TAYLOR Todd D, 五十君靜信, 服部正平

    日本乳酸菌学会誌   23 ( 2 )   120   2012年7月

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    記述言語:日本語  

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  • 小児消化管機能障害患児における網羅的腸内細菌叢解析の意義

    金森 豊, 杉山 正彦, 古村 眞, 寺脇 幹, 鈴木 完, 岩中 督, 須田 亙, 大島 健志朗, 服部 正平, 中野 章代, 森田 英利

    日本小児外科学会雑誌   48 ( 3 )   471 - 471   2012年5月

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  • Lactobacillus caseiグループの比較ゲノム解析

    森田英利, 藤英博, 中野章代, 大島健志朗, 五十君靜信, 服部正平

    日本畜産学会大会講演要旨   115th   189   2012年3月

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  • 免疫細胞を誘導するセグメント細菌(SFB)の全ゲノム解析

    大島健志朗, PRAKASH Tulika, 森田英利, 梅崎良則, 福田真嗣, 服部正平

    細胞工学   31 ( 4 )   458 - 459   2012年3月

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  • 日本人腸内マイクロバイオーム遺伝子の特徴解明

    西嶋傑, 大島健志朗, 飯岡恵里香, 大森恵美, 金錫元, 金相完, 須田亙, 森田英利, 服部正平

    日本ゲノム微生物学会年会要旨集   6th   96   2012年

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  • 腸内細菌叢を指標としたオランウータン個体識別法の開発

    須田亙, 鈴木晃, 鈴木南水子, 飯岡恵里香, 大森恵美, 高山由紀子, 中野章代, 金錫元, 大島健志朗, 服部正平, 森田英利

    日本ゲノム微生物学会年会要旨集   6th   95   2012年

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  • 乳酸菌とビフィズス菌における線毛遺伝子群の解析

    森田英利, PRAKASH Tulika, 大島健志朗, 藤英博, TAYLOR Todd D, 五十君靜信, 服部正平

    日本ゲノム微生物学会年会要旨集   6th   61   2012年

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  • ヒト腸内細菌叢メタゲノム研究とヒト常在菌ゲノム解析

    金相完, 大島健志朗, 須田亙, 金錫元, 飯岡恵里香, 稲葉寛実, 大森恵美, 木内美沙, 進藤智絵, 高山由紀子, 中野章代, 服部恭江, 古谷恵子, SAID Heba S, 西島傑, 高安伶奈, 森田英利, 服部正平

    日本ゲノム微生物学会年会要旨集   6th   95   2012年

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  • 日本人腸内マイクロバイオームの多様性と特徴解明

    西嶋傑, 大島健志朗, 飯岡恵里香, 大森恵美, 木内美沙, KIM Seq‐Won, 森田英利, 服部正平

    日本分子生物学会年会プログラム・要旨集(Web)   35th   4P-0036 (WEB ONLY)   2012年

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  • Lactobacillus brevis KB290のゲノム解析と次世代シークエンサーを用いた同一株内の多型解析

    深尾匡憲, 大島健志朗, 森田英利, 須田亙, 金錫元, 鈴木重徳, 矢賀部隆史, 矢嶋信浩, 服部正平

    日本乳酸菌学会誌   22 ( 2 )   125   2011年7月

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  • ラット由来セグメント細菌(SFB)の全ゲノム解析と各種動物の消化管におけるSFBの分布

    長森隆, SRIVASTAVA T. P, 大島健志朗, 服部正平, 石川英司, 今岡明美, 中野章代, 梅崎良則, 森田英利

    日本乳酸菌学会誌   22 ( 2 )   123   2011年7月

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  • 腸管出血性大腸菌O157:H7感染死マウスモデルを用いたビフィズス菌による感染死抑止機構の解明

    福田真嗣, 藤英博, 長谷耕二, 大島健志朗, 中西裕美子, 吉村和敏, 戸邉亨, 鈴木徹, 伊藤喜久治, 菊地淳, 森田英利, 股部正平, 大野博司

    日本農芸化学会大会講演要旨集   2011   216   2011年3月

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  • 次世代シークエンサーを用いたヒト口腔細菌叢の16S rDNA解析

    SAID Heba, 須田亙, 中込滋樹, 知念寛, 太田博樹, 伊良波淳, 金城福則, 石田肇, 木村亮介, KIM Seok‐Won, 大島健志朗, KIM Sangwan, 飯岡恵里香, 稲葉寛実, 大森恵美, 木内美沙, 高山由紀子, 中野章代, 古谷恵子, 森田英利

    日本分子生物学会年会プログラム・要旨集(Web)   34th   2P-0087 (WEB ONLY)   2011年

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  • 次世代シークエンサーを用いたヒト腸内細菌叢メタゲノム研究

    大島健志朗, KIM Sangwan, 須田亙, KIM Seok‐Won, 西嶋傑, 飯岡恵里香, 稲葉寛実, 大森恵美, 木内美沙, 進藤智絵, 高山由紀子, 中野章代, 服部恭江, 古谷恵子, HEBA Said, 森田英利, 服部正平

    日本分子生物学会年会プログラム・要旨集(Web)   34th   2P-0085 (WEB ONLY)   2011年

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  • ヒト腸内細菌のゲノム情報に基づく機能解析と生体影響 : 第2報

    村上 賢, 森田 英利, 加藤 行男, 久松 伸, 大島 健志朗, 服部 正平, 藤 英博, 高見 英人, 五十君 靜信, 桑原 知巳, 高木 邦明

    麻布大学雑誌 = Journal of Azabu University   19-20 ( 19 )   121 - 123   2010年3月

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    記述言語:日本語   出版者・発行元:麻布大学  

    我々は, ヒト消化管からの分離株であるL. rhamnosus ATCC 53103 株(別名としてGG 株)のゲノムを配列決定した。ATCC 53103 株は,最もプロバイオティクス効果の研究されている菌株の1 つである。本菌株のゲノムサイズは,3.0 Mb の染色体を有し,2,836 のタンパク質をコードしていた。糖代謝・アミノ酸代謝とその輸送に関係するタンパク質の他に,注目に値する防衛機構に関する遺伝子を多く保有していた。比較のゲノム分析で,広範囲なシンテニーがL. rhamnosus ATCC 53103 株とその近縁種であるL. casei ATCC 334 株(チーズ分離株)の間でみられた。しかし,L. rhamnosus ゲノムには多数の挿入,すなわち,PTS-タイプ輸送機システム,配糖体ヒドロラーゼと転写制御因子のために遺伝子から成る遺伝子群を含み,腸環境への潜在的適合性を反映していた。ATCC 53103 株には多くの細胞表面付着タンパク質が予測された。さらに,そのゲノムは,付着性に対して機能的なSpaCBA 線毛をコード化していた。L. rhamnosus ゲノムのこれらの特徴は,消化管生存に関与して,腸の粘膜と微生物叢との相互作用に貢献していると推察された。

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  • Lactobacillus brevis KB290が保有するプラスミドと人工消化液耐性との関係

    深尾匡憲, 鈴木重徳, 矢賀部隆史, 矢嶋信浩, 中野章代, 森田英利, 大島健志朗, 服部正平

    日本農芸化学会大会講演要旨集   2010   282   2010年3月

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  • 次世代シークエンサーを用いた微生物ゲノムと細菌叢メタゲノム研究

    大島健志朗, 藤英博, 福田真嗣, 金錫元, 森田英利, 大野博司, 服部正平

    日本ゲノム微生物学会年会要旨集   4th   51   2010年3月

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  • ヒト腸内細菌叢メタゲノム研究と微生物ゲノム解析

    大島健志朗, 森田英利, 飯岡恵里香, 中野章代, 須田亙, KIM Seok‐won, 稲葉寛実, 進藤智絵, 服部恭江, 古谷恵子, 服部正平

    生化学   ROMBUNNO.3P-0804   2010年

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  • ヒト腸内細菌のゲノム情報に基づく機能解析と生体影響―第1報―

    村上賢, 森田英利, 加藤行男, 久松伸, 服部正平, 藤英博, 高見英人, 五十君靜信, 桑原知巳, 高木邦明

    麻布大学雑誌   17-18   239 - 241   2009年3月

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  • ヒト消化管から分離されたBifidobacterium属の新菌種候補

    森田英利, 小野田ひろみ, 中野章代, 藤英博, 大島健志朗, 田辺創一, 村上賢, 服部正平

    日本畜産学会大会講演要旨   110th   125   2009年3月

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  • 健常なサラブレッドの16S rRNA遺伝子配列に基づく腸内フローラ解析と生菌分離されたLactoacillus属細菌について

    森田英利, 村上賢, 藤英博, 高見英人, 大島健志朗, 中島文彦, 服部正平

    日本乳酸菌学会誌   19 ( 2 )   112   2008年6月

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  • 有用細菌(乳酸菌等)の全ゲノム塩基配列決定,比較ゲノム解析および有用遺伝子の機能解明 : 第5報

    森田 英利, 大島 健志朗, 藤 英博, 政岡 俊夫, 茅根 士郎, 和田 恭則, 有嶋 和義, 木内 明男, 坂田 亮一, 紫野 正雄, 内藤 博之, 斉藤 康秀, 西田 利穂, 印牧 信行, 滝沢 達也, 加藤 行男, 村上 賢, 福山 正文, 岸川 正剛, 久松 伸, 吉村 哲彦, 柴 忠義, 服部 正平

    麻布大学雑誌 = Journal of Azabu University   15-16 ( 15 )   305 - 308   2008年3月

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    記述言語:日本語   出版者・発行元:麻布大学  

    Bifidobacterium属は,高いプロバイオティクス効果および細菌の分類学のモデルケースとして興味深い状況にある。(独)理化学研究所バイオリソースセンター微生物材料開発室(JCM)に保存されているBifidobacterium属およびその近縁種で,ヒトを分解源とする基準株を検索したところ,9菌種(菌株)が候補としてあげられ,その全ゲノム解析を開始した。その菌種は,Table 1に示したとおりであるが,B. bifidum, B. breveおよびB. catenulatumの3菌種は全ゲノム配列を決定し,B. dentium, P. denticolens, S. inopinataおよびG. vaginalisの3菌種は,コンティグが15前後になっており,B. longumおよびb. scarcoviiについては,ショットガンライブラリの作製を完了した。各菌株について引き続きシークエンス作業を進め,全ゲノム解析後は,大規模なBifidobacterium属の比較ゲノム解析を行う予定である。

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  • プロバイオティクス乳酸菌Lactobacillus rhamnosusのゲノム解析

    藤英博, 大島健志朗, 村上賢, 五十君靜信, 服部正平, 森田英利

    生化学   1P-0687   2008年

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  • <b>乳酸菌のゲノム解析の進展</b>

    森田 英利, 藤 英博

    腸内細菌学雑誌   21   209 - 215   2007年8月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    DOI: 10.11209/jim.21.209

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  • Lactobacillus reuteriの産生する3‐ヒドロキシプロピオンアルデヒドのin vivo検出

    森田英利, 藤英博, 福田真嗣, 大島健志朗, 村上賢, 加藤行男, 久松伸, 政岡俊夫, 伊藤喜久治, 菊地淳, 服部正平

    日本乳酸菌学会誌   18 ( 2 )   86   2007年6月

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  • ヒト腸内常在大腸菌のゲノム解析

    藤英博, 大島健志朗, 豊田敦, 笹本洋志, 小椋義俊, 森田英利, 林哲也, 伊藤喜久治, 榊佳之, 服部正平

    生化学   1P-0666   2007年

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  • 比較ゲノム解析による魚類α溶血性連鎖球菌Lactococcus garvieaeの病原性遺伝子の解析

    森田英利, 藤英博, 大島健志朗, 村上賢, 川西路子, 鈴木武人, 中谷航平, 政岡俊夫, 服部正平

    日本乳酸菌学会誌   17 ( 1 )   69   2006年6月

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  • ブリ属魚類にα溶血性連鎖球菌症を引き起すLactococcus garvieaeの比較ゲノム解析

    村上賢, 藤英博, 森田英利, 川西路子, 加藤行男, 福岡秀雄, 和田恭則, 政岡俊夫, 大島健志朗, 服部正平

    日本水産学会大会講演要旨集   2006   260   2006年3月

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  • 有用細菌(乳酸菌等)の全ゲノム塩基配列決定,比較ゲノム解析および有用遺伝子の機能解明(第4報) (平成17年麻布大学公的研究助成金事業研究成果報告)

    森田 英利, 藤 英博, 大島 健志朗, 鈴木 武人, 政岡 俊夫, 福岡 秀雄, 茅根 士郎, 和田 恭則, 有嶋 和義, 木内 明男, 坂田 亮一, 紫野 正雄, 内藤 博之, 斉藤 康秀, 西田 利穂, 印牧 信行, 滝沢 達也, 加藤 行男, 村上 賢, 福山 正文, 岸川 正剛, 久松 伸, 吉村 哲彦, 芝 忠義, 服部 正平

    麻布大学雑誌   13   318 - 324   2006年

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    記述言語:日本語   出版者・発行元:麻布大学  

    我々は,Lactobacillus reuteri JCM 1112^T, Lactobacillus fermentum IFO 3956, Lactococcus garvieae(ブリに対する毒性株), Lactococcus garvieae(ブリに対する無毒株)およびLactobacillus rhamnosusの全ゲノム解析を完了した。そして,Bifidobacterium breve, Bifidobacterium bifidumおよびBifidobacterium catenulatumについては,全ゲノム配列を決定中である。プロバイオティクスとは,"宿主の健康に対して貢献する生きた微生物"と定義付けられている。本プロジェクトでは,L.reuteriのプロバイオティクス効果を分子レベルで解明することを目的としている。そこで,分類学的にL.reuteriと近縁であるにもかかわらず,プロバイオティクス効果のほとんど認められないL.fermentumについても全ゲノム解析を行った。両菌種の比較ゲノム解析の結果,L.reuteriのゲノムには,抗菌物質であるロイテリン合成系にかかわる遺伝子の48kbの"genomicisland"を有しており,哺乳動物の腸内フローラバランスに良い影響を及ぼしていると推察された。我々は,glycerol dehydratase subunit motif(PFAM PFO2286-PFO2288)として,L.reuteriゲノムに3つの遺伝子(LR1633-LR1635)を検出し,gupCDE(glycerol utilizing enzymes)と命名した。本研究では,そのgupCDE遺伝子破壊候補株(L.reuteri Δ gupCDE)を得て,PCR解析やglycerol dehydratase酵素活性などから,L.reuteri Δ gupCDEを認めた。今後,親株(L.reuteriJCM1112^T株)とL.reuteri Δ gupCDEのそれぞれノトバイオート(モノアイソレート)マウスを解析して,プロバイオティクス効果を明らかにしていく予定である。

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  • Lactobacillus属におけるグルコース代謝とプロバイオティクス効果に関わる遺伝子群についての比較ゲノム解析

    森田英利, 藤英博, 堀川洋, 大島健志朗, 岡田英二, 岡田英二, 鈴木武人, 村上賢, 加藤行男, 山下敦士, 滝沢達也, 福岡秀雄, 吉村哲彦, 政岡俊夫, 服部正平

    日本分子生物学会年会講演要旨集   28th   362   2005年11月

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  • α溶血性連鎖球菌Lactococcus garvieaeの比較ゲノム解析による病原性遺伝子の探索

    藤英博, 大島健志朗, 川西路子, 森田英利, 中谷航平, 鈴木武人, 村上賢, 服部正平

    日本分子生物学会年会講演要旨集   28th   153   2005年11月

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  • ビタミンB<sub>12</sub>合成(cob/cbi)遺伝子とロイテリン産生(pdu)遺伝子の細菌間での水平伝播の証明

    森田英利, 藤英博, 鈴木武人, 大島健志朗, 服部正平

    日本畜産学会大会講演要旨   104th   178   2005年3月

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    記述言語:日本語  

    J-GLOBAL

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  • 有用細菌(乳酸菌等)の全ゲノム塩基配列決定,比較ゲノム解析および有用遺伝子の機能解明(第3報) (平成16年麻布大学公的研究助成金事業研究成果報告)

    森田 英利, 堀川 洋, 鈴木 武人, 政岡 俊夫, 福岡 秀雄, 茅根 士郎, 和田 恭則, 有嶋 和義, 木内 明男, 坂田 亮一, 紫野 正雄, 内藤 博之, 斉藤 康秀, 西田 利穂, 印牧 信行, 滝沢 達也, 加藤 行男, 村上 賢, 福山 正文, 岸川 正剛, 久松 伸, 吉村 哲彦, 藤 英博, 大島 健志朗, 芝 忠義, 服部 正平

    麻布大学雑誌   11   247 - 251   2005年

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    記述言語:日本語   出版者・発行元:麻布大学  

    プロバイオティクス乳酸菌としてLactobacillus reunteri (2, 039, 414 bp),そしてその知見のほとんどみられないLactobacillus fermentum(2, 098, 685 bp)の全ゲノム配列を決定した。両菌種は絶対ヘテロ発酵型の乳酸菌としては初めての全ゲノム配列決定となった。Lactobacillus属には,他にホモ発酵型と条件的ヘテロ発酵型の乳酸菌があるが,上記両菌種では,解糖(EMP)経路では必須の6-phosphofructokinase遺伝子の欠損が明らかとなり,そのためペントース・リン酸経路しか機能しなかった。各ORF内で3つ目の塩基の置換が非常に多く,そのことが遺伝子の機能は変わらず,G+C含量に10%もの違いが生じることが認められた。L. reuteriのゲノム中で多くのmobile elementsを発見したが,それはLactobacillus属が環境への適合性を高くし,生存性を有利にするのに貢献していることが示唆された。

    CiNii Article

    CiNii Books

    J-GLOBAL

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    その他リンク: http://id.nii.ac.jp/1112/00004845/

  • Lactobacillus reuteri JCM1112<sup>T</sup>の全ゲノムシークエンスからみたプロバイオティクスに関わる遺伝子群の解析

    森田英利, 政岡俊夫, 堀川洋, 鈴木武人, 大島健志朗, 藤英博, 山下敦士, 村上賢, 服部正平

    日本分子生物学会年会プログラム・講演要旨集   27th   773   2004年11月

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    記述言語:日本語  

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  • Nitric oxide complex of iron(II) myoglobin converted from metmyoglobin by Staphylococcus xylosus

    H Morita, R Sakata, Y Nagata

    JOURNAL OF FOOD SCIENCE   63 ( 2 )   352 - 355   1998年3月

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    記述言語:英語   出版者・発行元:INST FOOD TECHNOLOGISTS  

    With Staphylococcus xylosus FAX-1, metmyoglobin in MRS broth (pH 5.8) was found to undergo conversion to hexacoordinate nitric oxide (NO) complex of Fe(II) myoglobin. When the pH of the MRS culture containing myoglobin changed from 5.8 to 4.0, it affected the conversion from hexacoordinate to pentacoordinate NO complex of Fe(II) myoglobin, This conversion process was reversible. Salami without nitrite or nitrate addition was prepared by inoculating S. xylosus FAX-1, and pentacoordinate NO complex of Fe(II) myoglobin (nitrosylmyoglobin formed in cured meat) was formed in the salami.

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  • Synthesis of nitric oxide from the two equivalent guanidino nitrogens of L-arginine by Lactobacillus fermentum

    H Morita, H Yoshikawa, R Sakata, Y Nagata, H Tanaka

    JOURNAL OF BACTERIOLOGY   179 ( 24 )   7812 - 7815   1997年12月

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    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    Ten strains of Lactobacillus fermentum that differed in origin converted metmyoglobin to nitrosylmyoglobin [a pentacoordinate nitric oxide (NO) complex of Fe(II) myoglobin] in MRS broth at pH 4.3. Of the 10 strains, L. fermentum IFO 3956 possessed the strongest capacity to convert metmyoglobin to nitrosylmyoglobin. This strain synthesizes NO enzymatically from the two equivalent guanidino nitrogens of L-arginine. To our knowledge, this demonstrates for the first time the production of NO synthesized from the guanidino nitrogens of L-arginine by lactic acid bacteria. IFO 3956 may possess a bacterial NO synthase.

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  • Reddening and bacteriological property of salami without addition of nitrite and nitrate using Staphylococcus carnosus and Staphylococcus xylosus as starter cultures

    Anim. Sci. Technol. (Jpn. )   68   787 - 796   1997年

  • Cloning of citrate permease gene of Lactococcus lactis subsp. lactis biovar diacetylactis NIAI N-7 and expression in citrate-negative lactococci

    H Morita, K Kamizono, S Nakamura, Y Fujita, R Sakata, Y Nagata, LL McKay

    MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL   52 ( 3 )   138 - 141   1997年

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    記述言語:英語   出版者・発行元:VOLKSWIRTSCHAFTLICHER VERLAG  

    The putative citrate permease (Cit-P) plasmid DNA (pN7CP) from Lactococcus lactis subsp. lactis biovar diacetylactis (L. diacetylactis) NIAI N-7 was cloned. Cit-P+ transformants possessed pIL253 with a 3.8-kb fragment (pKAK1) or a 2.0-kb fragment (pKAK7). No XbaI site was present in the 3.8-kb of Xbal-Xbal fragment from pN7CP. It may thus be possible to obtain the 2.0-kb fragment by deleting the 3.8-kb fragment. Assessment was made of citrate-transporting activity in transformants KAK1 and KAK7. Several Cit(-) lactococci were transformed by pKAK7. Although all their transformants except KK-7LM and KK-7SL of Lactococcus lactis subsp. lactis (L. lactis) LM0230 and NIAI SLN were incapable of transporting citrate, transformants KK-7LM and KK-7SL showed the Cit(+) phenotype and citrate-transporting activity. L. diacetylactis and L. lactis were classified under citrate metabolism. However, the present findings strongly indicate some strains of L. lactis to quite likely be the original citrate utilizers but simply to have lost the Cit-P gene.

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  • Red pigment of parma ham and bacterial influence on its formation

    H Morita, J Niu, R Sakata, Y Nagata

    JOURNAL OF FOOD SCIENCE   61 ( 5 )   1021 - 1023   1996年9月

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    記述言語:英語   出版者・発行元:INST FOOD TECHNOLOGISTS  

    The red pigment of Parma ham was compared with the myoglobin derivatives present in meat and meat products. Spectral patterns of 75% acetone extracts and electron spin resonance spectra from Parma ham differed from those of the myoglobin derivatives. Staphylococci isolated from Parma ham generated red myoglobin derivative from metmyoglobin. Model fermented sausage prepared by inoculation with the isolates developed a more desirable red color without nitrite or nitrate treatment. The red pigment of Parma ham and the model sausage appeared to be the same myoglobin derivative. The reddening of Parma ham is probably caused by the action of bacteria.

    DOI: 10.1111/j.1365-2621.1996.tb10924.x

    Web of Science

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  • Metmyoglobin conversion to red myoglobin derivatives and citrate utilization by bacteria obtained from meat products and pickles for curing

    Anim. Sci. Technol. (Jpn. )   65   1026 - 1033   1994年

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講演・口頭発表等

  • アルツハイマー病患者の腸内細菌叢を移植したノトバイオートマウスの糞便中メタボローム解析 招待

    森田英利

    2021年1月23日 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(指名)  

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  • The salivary microbiome and immunoglobulin A nephropathy: A Japanese population-based study

    ○Khasnobish A, Suda W, Watanabe K, Nguyen TTT, Arakawa K, Hotta O, Joh K, Hattori M, Morita H

    第42回 日本分子生物学会年会  2019年12月5日 

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    記述言語:英語   会議種別:ポスター発表  

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  • “ 運動”が腸内フローラに及ぼす影響

    森田 英利

    第23回腸内細菌学会  2019年6月19日 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(指名)  

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  • 腸内細菌がなぜヒトと動物の健康に重要なのか 招待

    森田 英利

    平成30年度 日本獣医師会獣医学術学会年次大会  2019年2月9日 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(指名)  

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  • アルツハイマー病の腸内細菌叢を用いたヒト型ノトバイオートマウスの構築

    藤井 祐介, Thi Thuy Tien Nguyen, 藤村 勇太, 中村 昇二, 荒川 健佑, 森田 英利

    日本農芸化学会中四国支部第53回講演会(例会)  2019年1月26日 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:高知大学農林海洋科学部  

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  • コンディショニングにおける運動習慣と腸内フローラ 招待

    森田 英利

    日本食品免疫学会 第14回学術大会 JAFI2018  2018年11月16日 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • 腸内フローラとヒトの健康・疾病 招待

    森田 英利

    日本小児アレルギー学会第55回学術大会  2018年10月21日 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • アルツハイマー病と腸内細菌叢 招待

    森田 英利

    第37回日本認知症学会学術集会  2018年10月13日 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • ノトバイオート技術を用いた腸内細菌叢研究 招待

    森田 英利

    第45回 日本毒性学会学術年会  2018年7月19日 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • Gut Microbiota in Health and Disease 招待

    MORITA Hidetoshi

    ASEAN-JAPANゲノム医療研究推進会議(AGPC)  2018年7月10日 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • 腸内細菌叢の網羅的な解析と、その考え方に基づく最近の話題 招待

    森田 英利

    関西実験動物研究会第138回研究会  2018年6月22日 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • 網羅的腸内細菌叢解析を行った日本人糞便(消化管)から生菌分離したFaecalibacterium prausnitziiの特徴

    藤村勇太, 龍頭壮磨, 藤井祐介, Thi Thuy Tien Nguyen, 荒川健佑, 森田英利

    日本農芸化学会中四国支部第51回講演会(例会)  2018年6月16日 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:山口大学 吉田キャンパス  

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  • 保健機能食品制度におけるプロバイオティクス ・ プレバイオティクス 招待

    森田 英利

    プロバイオティクスシンポジウム '18  2018年5月11日 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • GUT MICROBIOTA OF JAPANESE ALZHEIMER'S DISEASE

    Gordon Research Conferences 2018  2018年2月13日 

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    記述言語:英語   会議種別:ポスター発表  

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産業財産権

  • 制御性T細胞の増殖または集積を誘導するヒト由来細菌

    本田 賢也, 新 幸二, 田ノ上 大, 服部 正平, 森田 英利

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    出願番号:特願2017-213526  出願日:2017年11月6日

    公開番号:特開2018-039833  公開日:2018年3月15日

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  • 糞便微生物叢を含む組成物

    垣花 和彦, 服部 正平, 本田 賢也, 西川 博嘉, 森田 英利, 竹下 梢

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    出願番号:特願2017-163690  出願日:2017年8月28日

    公開番号:特開2018-035153  公開日:2018年3月8日

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  • 自己免疫疾患の診断方法、自己免疫疾患の診断用バイオマーカー、及び自己免疫疾患の予防又は治療剤

    山村 隆, 服部 正平, 森田 英利, 三宅 幸子

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    出願番号:特願2017-536496  出願日:2016年8月26日

    公開番号:特開WO2017-034031  公開日:2018年6月14日

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  • イヌの歯周病の評価方法

    森田 英利, 菊水 健史, 髙木 智春, 浜川 弘茂, 明石 英子

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    出願番号:特願2015-218834  出願日:2015年11月6日

    公開番号:特開2017-085944  公開日:2017年5月25日

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  • ヒト唾液細菌叢の検査によるIgA腎症の診断方法

    森田 英利, 服部 正平

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    出願番号:特願2013-216247  出願日:2013年10月17日

    公開番号:特開2015-077101  公開日:2015年4月23日

    特許番号/登録番号:特許6296747  発行日:2018年3月2日

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  • 炎症性腸疾患の検出方法及びヒト唾液細胞叢の検査方法

    服部 正平, 太田 博樹, 森田 英利, 知念 寛

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    出願番号:特願2012-050007  出願日:2012年3月7日

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  • HUMAN-DERIVED BACTERIA THAT INDUCE PROLIFERATION OR ACCUMULATION OF REGULATORY T CELLS

    Honda K, Atarashi K, Tanoue T, Hattori M, Morita H

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    出願番号:特願61/607,360  出願日:2012年3月6日

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  • 病原性ラクトコッカス・ガルビエの検出方法

    森田 英利

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    出願番号:特願2008-067113  出願日:2008年3月17日

    公開番号:特開2009-148242  公開日:2009年7月9日

    特許番号/登録番号:特許5373301  発行日:2013年9月27日

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  • 新規なラクトバチルス属微生物および乳酸菌製剤

    森田 英利, 白鳥 千春

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    出願番号:特願2007-269758  出願日:2007年10月17日

    公開番号:特開2008-212140  公開日:2008年9月18日

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  • ダイオキシン類駆除促進剤

    篠田 直, 増山 明弘, 森田 英利, 吉川 宏

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    出願番号:特願2004-508827  出願日:2003年5月30日

    公開番号:特開WO2003-101472  公開日:2005年9月29日

    特許番号/登録番号:特許4652050  発行日:2010年12月24日

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受賞

  • 優秀発表賞

    2021年3月   日本畜産学会   野生シカから分離・選抜したチーズ用スターター乳酸菌の評価

    野崎颯馬, 福島渉, 細川美伊, 安達魁人, 大森遥, 坂本夏帆, 黒岡紗季, 安藤悠佳, 森田英利, 荒川健佑

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  • B.B.B.論文賞

    2020年2月   日本農芸化学会   Fecal metabolite of a gnotobiotic mouse transplanted with gut microbiota from a patient with Alzheimer’s disease

    Yusuke Fujii, Thuy Tien Thi Nguyen, Yuta Fujimura, Naotaka Kameya, Shoji Nakamura, Kensuke Arakawa, Hidetoshi Morita

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共同研究・競争的資金等の研究

  • 奄美群島の百寿者がもつ特徴的な腸内細菌叢を指標としたヒト長寿腸内科学への展開

    2018年06月 - 2020年03月

    学術振興会 科研費  挑戦的研究(萌芽) 

    森田 英利

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    担当区分:研究代表者  資金種別:競争的資金

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  • ノトバイオート技術を基盤としたアルツハイマー型認知症の細菌叢異常が示す生体影響

    2016年04月 - 2018年03月

    学術振興会 科研費  挑戦的萌芽研究 

    森田 英利

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    担当区分:研究代表者  資金種別:競争的資金

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  • ノトバイオート技術を基盤としたハダカデバネズミの真社会性制御機構の解明

    2015年04月 - 2018年03月

    学術振興会 科研費  基盤研究(B) 

    森田 英利

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    担当区分:研究代表者  資金種別:競争的資金

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  • 腸内フローラを用いた動物個体識別法の開発

    2011年04月 - 2013年03月

    学術振興会 科研費  挑戦的萌芽研究 

    森田 英利

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    担当区分:研究代表者  資金種別:競争的資金

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  • グリセロールに対するL. reuteriの遺伝子発現様式のin vivo解析

    2008年04月 - 2010年03月

    学術振興会 科研費  基盤研究(C) 

    森田 英利

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    担当区分:研究代表者  資金種別:競争的資金

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  • L.reuteriのロイテリン合成酵素遺伝子破壊株作製とプロバイオティクス評価

    2006年04月 - 2008年03月

    学術振興会 科研費  基盤研究(C) 

    森田 英利

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    担当区分:研究代表者  資金種別:競争的資金

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  • 乳酸菌のロイテリン産生経路における分子機構の解明

    2003年04月 - 2005年03月

    学術振興会 科研費  基盤研究(C) 

    森田 英利

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    担当区分:研究代表者  資金種別:競争的資金

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  • ヒト腸管定着性増強を目的とした乳酸菌での腸管定着性因子FimHタンパク質の発現

    1999年04月 - 2001年03月

    学術振興会 科研費  奨励研究(A) 

    森田 英利

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    担当区分:研究代表者  資金種別:競争的資金

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  • クエン酸発酵性乳酸菌のクエン酸分解酵素遺伝子のクローニングと他の乳酸菌での発現

    1994年04月 - 1995年03月

    学術振興会 科研費  奨励研究(A) 

    森田 英利

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    担当区分:研究代表者  資金種別:競争的資金

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  • 乳酸菌におけるクエン酸透過酵素およびクエン酸分解酵素遺伝子のクローニングと解析

    1993年04月 - 1994年03月

    学術振興会 科研費  奨励研究(A) 

    森田 英利

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    担当区分:研究代表者  資金種別:競争的資金

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担当授業科目

  • Food and Nutrition Science (2021年度) 第4学期  - 金1,金2

  • コース演習1 (2021年度) 1・2学期  - 水3

  • コース演習2 (2021年度) 3・4学期  - 水3

  • コース演習4 (2021年度) 3・4学期  - その他

  • メタボリックシンドローム特論 (2021年度) 特別  - その他

  • 動物応用微生物学 (2021年度) 3・4学期  - 木7,木8

  • 動物応用微生物学演習 (2021年度) 前期  - その他

  • 動物応用微生物学演習 (2021年度) 後期  - その他

  • 動物応用微生物学演習 (2021年度) 後期  - その他

  • 動物応用微生物学演習 (2021年度) 前期  - その他

  • 動物応用微生物学特論 (2021年度) 前期  - その他

  • 動物応用微生物学特論 (2021年度) 後期  - 金3,金4

  • 動物応用微生物学1 (2021年度) 第3学期  - 木7,木8

  • 動物応用微生物学2 (2021年度) 第4学期  - 木7,木8

  • 卒業論文 (2021年度) 1~4学期  - その他

  • 栄養生化学1 (2021年度) 第3学期  - 金5,金6

  • 生物生産科学特別研究 (2021年度) 通年  - その他

  • 生物生産科学特別研究 (2021年度) 通年  - その他

  • 生物生産科学特論I (2021年度) 夏季集中  - その他

  • 畜産食品機能学演習 (2021年度) 前期  - その他

  • 畜産食品機能学演習 (2021年度) 後期  - その他

  • 研究科目演習1 (2021年度) 1・2学期  - その他

  • 研究科目演習2 (2021年度) 3・4学期  - その他

  • Advances in Animal Science (2021年度) 後期  - 金7,金8

  • Food and Nutrition Science(食品・栄養科学) (2021年度) 第4学期  - 金1,金2

  • Food and Nutrition Science (2020年度) 第4学期  - 金1,金2

  • コース演習4 (2020年度) 3・4学期  - その他

  • 動物応用微生物学演習 (2020年度) 前期  - その他

  • 動物応用微生物学演習 (2020年度) 前期  - その他

  • 動物応用微生物学演習 (2020年度) 前期  - その他

  • 動物応用微生物学演習 (2020年度) 後期  - その他

  • 動物応用微生物学演習 (2020年度) 後期  - その他

  • 動物応用微生物学演習 (2020年度) 後期  - その他

  • 動物応用微生物学特論 (2020年度) 前期  - その他

  • 動物応用微生物学特論 (2020年度) 後期  - 金3,金4

  • 動物応用微生物学1 (2020年度) 第3学期  - 木7,木8

  • 動物応用微生物学2 (2020年度) 第4学期  - 木7,木8

  • 卒業論文 (2020年度) 1~4学期  - その他

  • 栄養生化学1 (2020年度) 第3学期  - 水5,水6

  • 生物生産科学特別研究 (2020年度) 通年  - その他

  • 生物生産科学特別研究 (2020年度) 通年  - その他

  • 畜産食品機能学演習 (2020年度) 前期  - その他

  • 畜産食品機能学演習 (2020年度) 後期  - その他

  • 研究科目演習1 (2020年度) 1・2学期  - その他

  • 研究科目演習2 (2020年度) 3・4学期  - その他

  • Food and Nutrition Science(食品・栄養科学) (2020年度) 第4学期  - 金1,金2

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社会貢献活動

  • 腸内細菌に操られるヒトの健康と疾病 -知られざる腸内フローラの秘密ー

    役割:講師

    日本成人病予防協会  2019年2月2日

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    種別:講演会

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  • 腸内フローラに操られるヒトの疾病と健康

    役割:講師

    広島県府中地区医師会  【市民公開講座】感染症講演会  2018年10月5日

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    種別:講演会

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  • 腸内環境と健康や病気の関係

    役割:講師

    岡山県里庄町健康福祉課  岡山県里庄町愛育委員会研修会  2018年5月15日

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    種別:講演会

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  • 腸内フローラに操られるヒトの健康と疾病

    役割:講師

    岡山大学研究推進産学官連携機構  第61回岡大サイエンスカフェ  2017年6月13日

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    種別:サイエンスカフェ

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メディア報道

  • 新われらの時代に「腸」 新聞・雑誌

    株式会社 小学館  女性セブン  p.56-p.57  2021年9月

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  • ヒューマニエンス「”腸内細菌”ヒトを飛躍させる生命体」 テレビ・ラジオ番組

    NHK BSプレミアム  2021年7月22日

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  • 腸を整える 新聞・雑誌

    株式会社マガジンハウス  クロワッサン  p.16-p.17  2021年5月

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  • 家庭の予防医学4 脳と腸 新聞・雑誌

    株式会社マガジンハウス  Tarzan(ターザン)  p.34-p.39  2020年10月

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  • 「太りやすい腸」にする?糖質制限 新聞・雑誌

    朝日新聞Reライフプロジェクト  朝日新聞 p.27  広告特集  2020年7月26日

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