Updated on 2024/03/20

写真a

 
MORIZANE Shin
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Professor
Position
Professor
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Degree

  • 医学博士 ( 岡山大学 )

Research Interests

  • kallikrein-related peptidases

  • antimicrobial peptides

  • epidermal keratinocytes

  • Innate immunity in the skin

  • Netherton syndrome

  • atopic dermatitis

  • psoriasis

  • LEKTI

Research Areas

  • Life Science / Dermatology

Education

  • Okayama University   医学系研究科   病態制御科学

    - 2006

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    Country: Japan

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  • Okayama University   医学部   医学科

    - 2000

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    Country: Japan

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Research History

  • 岡山大学大学院医歯薬学総合研究科   教授

    2018.7

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  • Okayama University   Okayama University Hospital   Lecturer

    2018.1 - 2018.6

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  • Okayama University   Okayama University Hospital   Assistant Professor

    2009 - 2017

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  • 米国カリフォルニア大学サンディエゴ校医学部皮膚科学講座 ポストドクトラルフェロー

    2007 - 2009

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Professional Memberships

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Committee Memberships

  • 岡山県アレルギー疾患医療連絡協議会   委員  

    2022.11   

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  • 日本乾癬学会   理事  

    2022.10   

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    Committee type:Academic society

    日本乾癬学会

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  • 日本皮膚科学会   代議員  

    2019.4   

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  • 日本皮膚免疫アレルギー学会   代議員  

    2015.4   

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    Committee type:Academic society

    日本皮膚アレルギー・接触皮膚炎学会

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  • 日本研究皮膚科学会   評議員  

    2013.4   

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    Committee type:Academic society

    日本研究皮膚科学会

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Papers

  • A method for measuring serum levels of melanin-associated indole metabolites using LC-MS/MS and its application to malignant melanoma. International journal

    Masaki Takiwaki, Hiroshi Umemura, Yoshikuni Kikutani, Seketsu Fukuzawa, Kentaro Abe, Kiyotaka Fujino, Satoru Sugihara, Kota Tachibana, Shin Morizane, Mamoru Satoh, Tomohiro Nakayama, Osamu Yamasaki

    Clinica chimica acta; international journal of clinical chemistry   117873 - 117873   2024.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND AND AIMS: With the development of novel therapies for advanced malignant melanoma (MM), biomarkers that can accurately reflect the progression of MM are needed. Serum levels of melanin-related indole metabolites such as 5-hydroxy-6-methoxyindole-2-carboxylic acid (5H6MI2C) and 6-hydroxy-5-methoxyindole-2-carboxylic acid (6H5MI2C) are potential biomarkers for MM. Here, we describe the development of a mass spectrometry (MS)-based assay to determine serum levels of 5H6MI2C and 6H5MI2C. MATERIALS AND METHODS: We developed a stable isotope dilution-selective reaction monitoring-MS protocol using liquid chromatography tandem mass spectrometry (LC-MS/MS) to measure human serum 5H6MI2C and 6H5MI2C levels. Analytical evaluations of the method were performed and the method was applied to serum samples from MM patients (n = 81). RESULTS: The method established in this study showed high reproducibility and linearity. This novel method also found that serum 6H5MI2C levels were significantly elevated in patients with metastatic MM compared to those with non-metastatic MM. Unfortunately, 5H6MI2C did not show a comparable significant difference. CONCLUSION: We successfully established measurement methods for serum 5H6MI2C and 6H5MI2C levels using LC-MS/MS. Serum 6H5MI2C levels offer a potential marker for MM.

    DOI: 10.1016/j.cca.2024.117873

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  • Pruritic folliculitis of pregnancy with granular deposition of immunoglobulin G along the basement membrane zone. International journal

    Mana Usui-Taniguchi, Yoshio Kawakami, Yoichiro Toi, Tatsuya Kaji, Yoshiko Matsuura, Emi Yokoyama, Tomoko Miyake, Yoji Hirai, Shin Morizane

    The Journal of dermatology   2023.11

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  • 【生活習慣が関連する皮膚疾患】生活習慣を見直すことで治癒したElephantiasis Nostrasの1例

    廣瀬 梓, 川上 佳夫, 長尾 聡子, 三宅 智子, 森実 真

    皮膚科の臨床   65 ( 11 )   1645 - 1647   2023.10

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    Language:Japanese   Publisher:金原出版(株)  

    <文献概要>32歳,女性。発熱,両下腿の発赤・腫脹を主訴に受診した。肥満があり,両側下腿にnon-pitting edemaとびらん形成があり,右下腿には疣状・乳頭状増殖が認められ,黄白色の浸軟した痂皮が付着していた。うっ滞性皮膚炎によるelephantiasis nostrasから発症した右下腿蜂窩織炎と診断した。入院のうえ,セファゾリン点滴,ゲンタマイシン軟膏外用,弾性包帯による圧迫療法を開始したところ,感染徴候は軽快した。入院中の問診で,中学卒業以降引きこもり状態にあり,日中は立位で家事に従事し,夜間は部屋が狭いために座位で睡眠をとっていたことが発覚した。下肢挙上の重要性を説明し,臥位による睡眠と運動などの生活指導を行った結果,elephantiasis nostrasやうっ滞性皮膚炎の症状も軽快した。Elephantiasis nostrasの発症には生活習慣など社会的背景が関与していることが多い。生活環境の是正も含めて経過をみる必要がある。

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  • 酒さ様皮膚炎の経過に生じたMorbihan病と考えられた1例

    横溝 紗佑里, 横山 恵美, 川上 佳夫, 森実 真, 安井 陽子

    西日本皮膚科   85 ( 5 )   347 - 350   2023.10

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    Language:Japanese   Publisher:日本皮膚科学会-西部支部  

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  • Cutaneous toxicity with subepidermal blisters and dyskeratosis following administration of pemetrexed in a patient with nivolumab-induced psoriasis and linear IgA bullous dermatosis. International journal

    Sayuri Yokomizo, Tomoko Miyake, Yoshio Kawakami, Kadoaki Ohashi, Hiroshi Koga, Norito Ishii, Shin Morizane

    The Journal of dermatology   2023.9

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  • Vulvar Crohn's disease presenting with multiple exophytic nodules and labial swelling: A case report. International journal

    Ai Kajita, Yoshio Kawakami, Sakiko Hiraoka, Junko Haraga, Shin Morizane

    The Journal of dermatology   2023.8

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  • Successful treatment with secukinumab in a patient with generalized pustular psoriasis preceded by palmoplantar lesions. International journal

    Nozomi Sawai, Yoshio Kawakami, Kota Tachibana, Hayato Nomura, Tomoko Miyake, Emi Yokoyama, Yoji Hirai, Shin Morizane

    The Journal of dermatology   2023.8

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  • 【指定難病Up to Date】表皮水疱症

    三宅 智子, 森実 真

    岡山医学会雑誌   135 ( 2 )   78 - 80   2023.8

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    Language:Japanese   Publisher:岡山医学会  

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  • 頭頸部の皮疹が顕著でセクキヌマブが奏効した尋常性乾癬の小児例

    松田 吉弘, 川上 佳夫, 八代 将登, 森実 真

    臨床皮膚科   77 ( 9 )   695 - 700   2023.8

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    Language:Japanese   Publisher:(株)医学書院  

    <文献概要>14歳,男児.2歳時に両膝に皮疹が生じ,全身に拡大した.3歳時に皮膚生検で尋常性乾癬と診断され,ステロイド外用や活性型ビタミンD3外用にて加療されるも難治であり,8歳時に当科を紹介された.ナローバンドUVB療法に抵抗性を示し,シクロスポリン2.5mg/kg/日内服は有効であったが,減量により皮疹は再燃した.滴状乾癬様の皮疹も認めたため,アモキシシリンの内服や齲歯治療,扁桃摘出を行うも皮疹は軽快しなかった.14歳時にセクキヌマブを導入したところ(セクキヌマブ導入前PASI 11.7),1週後にはPASI 2.4と速やかに改善し,12週後にPASIクリアを達成した.自験例では頭頸部の皮疹が顕著で日常生活に支障をきたしていた.小児の乾癬で皮疹が露出部にある場合には患者のQOLも考慮して治療方針を立てる必要がある.その一方で,小児の乾癬に対する生物学的製剤の長期投与については,安全性に関する情報が少ないため,副作用の発現に留意してフォローする必要がある.

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  • Angioedema‐like eyelid edema following the second NVX‐CoV2373 <scp>COVID</scp>‐19 vaccination

    Mari Matsumoto, Yoshio Kawakami, Tomoko Miyake, Yoji Hirai, Hitomi Kataoka, Fumio Otsuka, Shin Morizane

    Journal of Cutaneous Immunology and Allergy   6 ( 5 )   194 - 195   2023.7

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    Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    A 43‐year‐old woman developed angioedema‐like eyelid edema on day +6 after the second NVX‐CoV2373 vaccination. Short‐term oral prednisolone treatment resulted in complete resolution of the eyelid edema.

    DOI: 10.1002/cia2.12314

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  • Coexpression of natural killer cell antigens by T-cell large granular lymphocytes in hydroa vacciniforme lymphoproliferative disorder and the involvement of Vδ1 + epithelial-type γδT cells.

    Yoji Hirai, Keiji Iwatsuki, Takahide Takahashi, Tomoko Miyake, Yuki Nakagawa, Shogo Tanimoto, Yoshio Kawakami, Shin Morizane

    International journal of hematology   118 ( 1 )   54 - 64   2023.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    Hydroa vacciniforme lymphoproliferative disorder (HV-LPD) is a cutaneous variant of chronic active Epstein-Barr virus disease. We examined the coexpression of T- and natural killer (NK)-cell antigens in five patients with classic HV (cHV) and five with systemic HV (sHV). T-cell receptor (TCR) repertoire analysis was performed with high‑throughput sequencing. All five cHV patients had increased γδT cells (> 5%), whereas five sHV patients showed γδT- and αβT-cell dominance in two patients each, and a mixture of abnormal γδT and αβT cells in one. Circulating CD3 + T cells expressed CD16/CD56 at 7.8-42.3% and 1.1-9.7% in sHV and cHV, respectively. The percentage of CD16/CD56 + T cells was higher in the large granular lymphocyte or atypical T-cell fractions in sHV, but no TCR Vα24 invariant chain characteristic of NKT cells was detected. Considerable numbers of CD3 + cells expressing CD56 were observed in sHV skin infiltrates. Of the circulating γδT cells tested, TCR Vδ1 + cells characteristic of the epithelial type of γδT cells were dominant in two sHV cases. Thus, atypical αβT and γδT cells in HV-LPD can express NK-cell antigens, such as CD16 and CD56, and Vδ1 + epithelial-type γδT cells are a major cell type in some HV-LPD cases.

    DOI: 10.1007/s12185-023-03599-7

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  • 口腔・鼻粘膜びらんを初発症状とした水疱型エリテマトーデスの1例

    浦上 仁志, 三宅 智子, 篠倉 美里, 森田 安理, 竹崎 大輝, 岡野 真理, 川上 佳夫, 森実 真, 古賀 浩嗣, 石井 文人

    西日本皮膚科   85 ( 3 )   234 - 235   2023.6

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    Language:Japanese   Publisher:日本皮膚科学会-西部支部  

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  • 【乾癬】ブロダルマブ自己中断後に膿疱化を生じた乾癬性関節炎の1例

    廣瀬 梓, 川上 佳夫, 吉永 泰彦, 三宅 智子, 森実 真

    皮膚科の臨床   65 ( 5 )   569 - 572   2023.5

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    Language:Japanese   Publisher:金原出版(株)  

    <文献概要>71歳,男性。乾癬性関節炎に対してアダリムマブで加療するも二次無効となり,紅皮症化したためブロダルマブを開始した。その後皮膚症状,関節症状ともに速やかに改善したが,2ヵ月間の自己中断後に発熱,関節痛,皮疹の膿疱化を認めた。ブロダルマブを再開し,速やかに皮疹は改善した。今後も治療を自己中断する懸念があり,ブロダルマブをグセルクマブに変更した。しかし,関節症状が再燃したため,さらにセルトリズマブペゴルに変更し,皮膚,関節症状ともに改善した。抗IL-17受容体A抗体であるブロダルマブは,中止後にリバウンド現象として膿疱化が出現することがあるため,治療を自己中断しないよう厳格に指導する必要がある。

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    Other Link: https://search.jamas.or.jp/default/link?pub_year=2023&ichushi_jid=J01266&link_issn=&doc_id=20230524110011&doc_link_id=10.18888%2Fhi.0000003891&url=https%3A%2F%2Fdoi.org%2F10.18888%2Fhi.0000003891&type=%E5%8C%BB%E6%9B%B8.jp_%E3%82%AA%E3%83%BC%E3%83%AB%E3%82%A2%E3%82%AF%E3%82%BB%E3%82%B9&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00024_2.gif

  • 鼠径リンパ節郭清後に発症したAnesthesia Mumpsの1例

    廣瀬 梓, 杉原 悟, 三宅 智子, 山崎 修, 森実 真, 片岡 祐子, 松崎 孝

    皮膚科の臨床   65 ( 5 )   686 - 687   2023.5

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    症例は47歳女性で、左鼠径部に3cm大の皮下腫瘤を自覚し、CTでリンパ節腫脹を指摘されたため前医外科を受診した。その後、原発不明悪性黒色腫の疑いで当院を紹介受診し、左鼠径リンパ節郭清を全身麻酔下で施行した。約6時間後に夕食を摂取し、その30分後に両耳介周囲の腫脹が出現、麻酔科、耳鼻科と併診した。麻酔科では手技や術後経過は麻酔科的に問題がないと考えられ、アナフィラキシーのような全身症状や皮膚症状もなく、限局した腫脹のみで使用した薬剤との関連は明らかではなかった。耳鼻科では臨床症状、採血結果から細菌感染症、ウイルス感染症の可能性は低いと判断された。アレルギー性耳下腺炎を疑われベポタスチンの内服を開始し、発症から3日で症状が軽快し、血清アミラーゼ値も正常化したためベポタスチンの内服は終了とした。以降症状の再燃はなかった。鼠径リンパ節の病理診断は悪性黒色腫の転移であった。

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  • 濾胞性リンパ腫と閉塞性細気管支炎を合併した腫瘍随伴性天疱瘡の1例

    赤松 由規, 三宅 智子, 藤井 江利子, 川本 友子, 山崎 修, 森実 真, 鳥越 利加子, 大山 矩史, 藤原 英晃, 石井 文人

    皮膚科の臨床   65 ( 5 )   632 - 636   2023.5

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    Language:Japanese   Publisher:金原出版(株)  

    <文献概要>67歳,女性。初診1.5ヵ月前より体重減少と口唇・口腔内にびらんが出現した。上肢と体幹には爪甲大の痂皮と紅斑を認めた。CTで後腹膜と鼠径部に腫瘤を認め,生検にて濾胞性リンパ腫と診断した。皮膚生検では苔癬型反応を認め,血清中の抗Dsg3抗体,抗エンボプラキン抗体,抗ペリプラキン抗体が陽性で腫瘍随伴性天疱瘡と診断した。オビヌツズマブ,ベンダムスチンを投与し腫瘍,皮膚粘膜症状ともに改善していたが,閉塞性細気管支炎を合併した。発症10ヵ月後に肺気胸と肺炎により死亡した。腫瘍随伴性天疱瘡に合併する閉塞性細気管支炎は腫瘍縮小,皮膚粘膜病変改善後も発症・進行し致死的な経過をたどる傾向があり,呼吸状態の変化に留意する必要がある。

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    Other Link: https://search.jamas.or.jp/default/link?pub_year=2023&ichushi_jid=J01266&link_issn=&doc_id=20230524110025&doc_link_id=10.18888%2Fhi.0000003905&url=https%3A%2F%2Fdoi.org%2F10.18888%2Fhi.0000003905&type=%E5%8C%BB%E6%9B%B8.jp_%E3%82%AA%E3%83%BC%E3%83%AB%E3%82%A2%E3%82%AF%E3%82%BB%E3%82%B9&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00024_2.gif

  • 四肢と左臀部に生じたextensive subcutaneous sarcoidosisの1例

    臼井 真菜, 杉原 悟, 立花 宏太, 安富 陽平, 川上 佳夫, 中本 健太, 大塚 文男, 森実 真

    西日本皮膚科   85 ( 2 )   109 - 112   2023.4

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    Language:Japanese   Publisher:日本皮膚科学会-西部支部  

    71歳,女性。1年前に右膝と両側前腕に皮下硬結を自覚し,その後左臀部にも皮下硬結が出現した。右膝に20×10cm,左臀部に10×7cm,右前腕に10×7cm,左前腕に2個の7×5cmの板状皮下硬結がみられた。皮膚生検では皮下脂肪組織に非乾酪壊死性類上皮細胞肉芽腫を認め,sIL-2Rは1697.0U/mlと高値であった。胸部CTでは縦隔リンパ節の軽度腫大があり,Gaシンチグラフィーでは四肢と左臀部の皮下硬結部に高度な集積を認め,extensive subcutaneous sarcoidosisと診断した。膝関節屈曲制限が生じる懸念があったためプレドニゾロン25mg/day内服を開始したところ,皮下病変は速やかに縮小し,sIL-2Rは418.3U/mlまで低下した。皮下型サルコイドーシスではトラニラストやミノサイクリン内服で軽快する症例や,無治療で軽快する症例もあるが,本症例では,右膝部皮下病変の拡大による歩行障害が懸念されたため,ステロイド内服による治療を選択した。広範な皮下型病変を呈するサルコイドーシスでは,sIL-2Rや皮膚病変のサイズなどを総合的に評価し,病勢を検討することが必要である。(著者抄録)

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    Other Link: https://search.jamas.or.jp/default/link?pub_year=2023&ichushi_jid=J01003&link_issn=&doc_id=20230516250006&doc_link_id=10.2336%2Fnishinihonhifu.85.109&url=https%3A%2F%2Fdoi.org%2F10.2336%2Fnishinihonhifu.85.109&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_2.gif

  • Clinical characteristics of patients with psoriasis with family history: A multicenter observational study

    Chika Ohata, Hisataka Anezaki, Sakae Kaneko, Fusako Okazaki, Kotaro Ito, Yuki Matsuzaka, Satoko Kikuchi, Yuta Koike, Hiroyuki Murota, Takuya Miyagi, Kenzo Takahashi, Kazunari Sugita, Aki Hashimoto, Takeshi Nakahara, Shin Morizane, Bungo Ohyama, Hiroshi Saruwatari, Tetsuji Yanase, Yuichi Yoshida, Kentaro Yonekura, Yuko Higashi, Yutaka Hatano, Kanami Saito, Eri Katayama, Mariko Yamane, Fumikazu Yamazaki, Noriko Tsuruta, Shinichi Imafuku

    Journal of Dermatology   2023

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    Previous studies on family history of psoriasis showed that patients with a family history have an earlier onset of the disease, but such studies in Japan are still limited. To elucidate the characteristics of patients with familial psoriasis, we studied the family history of patients with psoriasis using the West Japan Psoriasis Registry, a multi-institutional registry operated by 26 facilities in the western part of Japan, including university hospitals, community hospitals, and clinics. This study enrolled 1847 patients registered between September 2019 and December 2021, with 199 (10.8%) having a family history of psoriasis. Patients with a family history of psoriasis had significantly earlier onset of the disease than those without a family history. Furthermore, patients with a family history of psoriasis had significantly longer disease duration. Psoriatic arthritis (PsA) was significantly more common in patients with a family history (69/199, 34.7%) than in those without a family history (439/1648, 26.6%) (adjusted P = 0.023). A subanalysis of patients with PsA revealed a significant difference in the patient global assessment (PaGA) score in Fisher's exact test and adjusted test. The numbers of patients with PaGA 0/1 were 29 (43.3%) and 172 (39.9%) in patients with PsA with and without family history of psoriasis, respectively, whereas the numbers of patients with PaGA 3/4 were 13 (19.4%) and 145 (33.6%) in patients with PsA with and without family history of psoriasis, respectively. Other disease severity variables did not show a difference between the two groups. Our findings suggest that genetics play a larger role in the development of PsA than in the development of psoriasis vulgaris. Most cases of PsA occur in patients who already have psoriasis, therefore dermatologists should pay attention to joint symptoms, especially in patients with psoriasis who have a family history of psoriasis.

    DOI: 10.1111/1346-8138.16733

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  • Cutaneous Pseudolymphoma Caused by an Injury with a Metallic Piece of a Mowing Machine Containing Tungsten. International journal

    Mayu Tokuda, Yoshio Kawakami, Yoji Hirai, Kota Tachibana, Yuki Nakagawa, Satoru Sugihara, Tomoko Miyake, Osamu Yamasaki, Keiko Manabe, Shin Morizane

    Indian journal of dermatology   68 ( 4 )   468 - 470   2023

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    DOI: 10.4103/ijd.ijd_645_22

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  • Co-occurrence of non-alcoholic steatohepatitis exacerbates psoriasis associated with decreased adiponectin expression in a murine model. International journal

    Daiki Takezaki, Shin Morizane, Kenta Ikeda, Masanori Iseki, Yuma Sakamoto, Yoshio Kawakami, Taishi Hashiguchi, Yuka Shirakata, Sohji Nishina, Tomoyuki Mukai

    Frontiers in immunology   14   1214623 - 1214623   2023

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    INTRODUCTION: Clinical studies have suggested a bidirectional association between non-alcoholic steatohepatitis (NASH) and psoriasis, affecting each other's development and severity. Here, we explored bidirectional causal linkages between NASH and psoriasis using a murine model. METHODS: NASH was induced in mice by streptozotocin injection at 2 days of age and by high-fat diet feeding (STAM™ model). Psoriasis was induced by topical application of imiquimod (IMQ) on the ear. The severities of liver damage and psoriatic skin changes were determined using histological analysis. Gene expression in the skin tissues was evaluated using quantitative PCR analysis. Serum cytokine levels were determined using enzyme-linked immunosorbent assay. To examine the innate immune responses of normal human epidermal keratinocytes (NHEKs), the cells were treated with interleukin (IL)-17A, tumor necrosis factor (TNF)-α, and AdipoRon, an adiponectin receptor agonist. RESULTS AND DISCUSSION: There were no differences in the degree of liver tissue damage (fat deposition, inflammation, and fibrosis) between NASH mice with and those without psoriasis. Conversely, the co-occurrence of NASH significantly augmented psoriatic skin changes, represented by epidermal hyperplasia, in psoriatic mice. Pro-inflammatory cytokines were expressed in the inflamed skin of psoriatic mice, and the expression of genes, especially Il23a, Il1b, Il36g, and Mip2, was significantly upregulated by the co-occurrence of NASH. The expression of keratinocyte activation marker genes Defb4b and Krt16 was also upregulated by the co-occurrence of NASH. The serum TNF-α and IL-17 levels were increased by the co-occurrence of NASH and psoriasis. The serum adiponectin levels decreased in NASH mice compared with that in non-NASH mice. In NHEK culture, TNF-α and IL-17A synergistically upregulated CXCL1, CXCL8, and IL1B expression. The upregulated pro-inflammatory gene expression was suppressed by AdipoRon treatment, reflecting the anti-inflammatory capacity of adiponectin. CONCLUSION: The co-occurrence of NASH exacerbated psoriatic skin changes associated with increased serum inflammatory cytokine levels and decreased serum adiponectin levels. Combined with in vitro findings, increased inflammatory cytokine levels and decreased adiponectin levels likely promote innate immune responses in epidermal keratinocytes in psoriatic skin lesions. Overall, therapeutic intervention for co-occurring NASH is essential to achieve a favorable prognosis of psoriasis in clinical practice.

    DOI: 10.3389/fimmu.2023.1214623

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  • "Input/output cytokines" in epidermal keratinocytes and the involvement in inflammatory skin diseases. International journal

    Shin Morizane, Tomoyuki Mukai, Ko Sunagawa, Kota Tachibana, Yoshio Kawakami, Mamoru Ouchida

    Frontiers in immunology   14   1239598 - 1239598   2023

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    Considering the role of epidermal keratinocytes, they occupy more than 90% of the epidermis, form a physical barrier, and also function as innate immune barrier. For example, epidermal keratinocytes are capable of recognizing various cytokines and pathogen-associated molecular pattern, and producing a wide variety of inflammatory cytokines, chemokines, and antimicrobial peptides. Previous basic studies have shown that the immune response of epidermal keratinocytes has a significant impact on inflammatory skin diseases. The purpose of this review is to provide foundation of knowledge on the cytokines which are recognized or produced by epidermal keratinocytes. Since a number of biologics for skin diseases have appeared, it is necessary to fully understand the relationship between epidermal keratinocytes and the cytokines. In this review, the cytokines recognized by epidermal keratinocytes are specifically introduced as "input cytokines", and the produced cytokines as "output cytokines". Furthermore, we also refer to the existence of biologics against those input and output cytokines, and the target skin diseases. These use results demonstrate how important targeted cytokines are in real skin diseases, and enhance our understanding of the cytokines.

    DOI: 10.3389/fimmu.2023.1239598

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  • A case of generalized allergic contact dermatitis due to sodium lauroyl methylaminopropionate in a shampoo. International journal

    Azusa Hirose, Yoshio Kawakami, Tomoko Miyake, Yoji Hirai, Shin Morizane

    Contact dermatitis   87 ( 6 )   546 - 548   2022.12

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    DOI: 10.1111/cod.14210

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  • Probability scoring system of intravascular large B-cell lymphoma for the application of random skin biopsy: A retrospective cohort study. International journal

    Mikiko Takigawa, Osamu Yamasaki, Hayato Nomura, Tomoko Miyake, Hiroyuki Yanai, Shin Morizane

    JAAD international   9   146 - 152   2022.12

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    BACKGROUND: Intravascular large B-cell lymphoma (IVLBCL) is rare and fatal when diagnosed late in the disease course. Random skin biopsy (RSB) is useful for early diagnosis, but criteria for its application are not well established. OBJECTIVE: To develop an IVLBCL-probability scoring system for stratifying patients and investigate its feasibility and capability for RSB application. METHODS: A retrospective cohort of 77 consecutive patients with suspected IVLBCL who underwent RSB was included in this study. All patients were classified into 3 IVLBCL-probability groups according to the IVLBCL-probability scoring system comprising the following 4 components: general symptoms, organ-specific symptoms, serum soluble-interleukin-2 receptor levels, and serum lactate-dehydrogenase levels. RESULTS: The high (score 7-10), intermediate (score 4-6) and low (score 1-3) IVLBCL-probability groups contained 32, 30, and 15 patients, respectively. All 5 patients with IVLBCL were stratified into the high IVLBCL probability group. Accuracies in the diagnosis of IVLBCL were 100%, 100%, and 93.8% for the low, intermediate, and high IVLBCL-probability groups. The positive detection rate in the high IVLBCL-probability group increased to 9.4% from 3.9% across all groups. CONCLUSIONS: The newly-developed IVLBCL-probability scoring system has good capability for stratification of patients and could allow limiting application of RSB for diagnosis only to high-probability groups.

    DOI: 10.1016/j.jdin.2022.09.005

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  • MRIにて大腿骨と腸骨に骨髄浮腫がみられた下肢の線状強皮症の1例

    佐藤 志帆, 梶田 藍, 山崎 修, 中原 龍一, 森実 真

    西日本皮膚科   84 ( 6 )   521 - 525   2022.12

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  • ペムブロリズマブ投与後IFNβ局注,イミキモド外用を併用した悪性黒色腫in-transit転移の1例

    山崎 修, 杉原 悟, 立花 宏太, 三宅 智子, 森実 真, 柳井 広之, 荒川 謙三

    西日本皮膚科   84 ( 6 )   570 - 571   2022.12

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  • シャンプーに含まれるN-ラウロイル-N-メチル-β-アラニンNaによる接触皮膚炎症候群の1例

    廣瀬 梓, 川上 佳夫, 三宅 智子, 平井 陽至, 森実 真

    日本皮膚免疫アレルギー学会総会学術大会プログラム・抄録集   52回   201 - 201   2022.12

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  • コロナワクチン接種後に生じたStevens-Johnson症候群の1例

    高須賀 琴巳, 川上 佳夫, 西山 進, 宮脇 昌二, 森実 真

    日本皮膚免疫アレルギー学会総会学術大会プログラム・抄録集   52回   194 - 194   2022.12

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  • Cutaneous toxicity with suprabasal blisters and dyskeratosis following administration of enfortumab vedotin. International journal

    Ken-Ichi Hasui, Yoshio Kawakami, Tomoko Miyake, Yoji Hirai, Hayato Nomura, Kohei Edamura, Motoo Araki, Shin Morizane

    The Journal of dermatology   2022.11

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  • Ceramide analysis in combination with genetic testing may provide a precise diagnosis for self-healing collodion babies. Reviewed International journal

    Takuya Takeichi, Yusuke Ohno, Kana Tanahashi, Yasutoshi Ito, Ken Shiraishi, Ryo Utsunomiya, Satoshi Yoshida, Kenta Ikeda, Hayato Nomura, Shin Morizane, Koji Sayama, Tomoo Ogi, Yoshinao Muro, Akio Kihara, Masashi Akiyama

    Journal of lipid research   63 ( 12 )   100308 - 100308   2022.11

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    Self-healing collodion baby (SHCB), also called "self-improving collodion baby", is a rare mild variant of autosomal recessive congenital ichthyosis and is defined as a collodion baby who shows the nearly complete resolution of scaling within the first 3 months to 1 year of life. However, during the neonatal period, it is not easy to distinguish SHCB from other inflammatory forms of autosomal recessive congenital ichthyosis, such as congenital ichthyosiform erythroderma. Here, we report a case study of two Japanese SHCB patients with compound heterozygous mutations, c.235G>T (p.(Glu79*))/ c.1189C>T (p.(Arg397Cys)) and c.1295A>G (p.(Tyr432Cys))/ c.1138delG (p.(Asp380Thrfs*3)), in CYP4F22, which encodes cytochrome P450, family 4, subfamily F, polypeptide 22 (CYP4F22). Immunohistochemically, inflammation with the strong expression of IL-17C, IL-36γ, and TNF-α was seen in the skin at birth. CYP4F22 is an ultra-long-chain fatty acid (FA) ω-hydroxylase responsible for ω-O-acylceramide (acylceramide) production. Among the epidermal ceramides, acylceramide is a key lipid in maintaining the epidermal permeability barrier function. We found that the levels of ceramides with ω-hydroxy FAs including acylceramides and the levels of protein-bound ceramides were much lower in stratum corneum samples obtained by tape stripping from SHCB patients than in those from their unaffected parents and individuals without SHCB. Additionally, our cell-based enzyme assay revealed that two mutants, p.(Glu79*) and p.(Arg397Cys), had no enzyme activity. Our findings suggest that genetic testing coupled with non-invasive ceramide analyses using tape-stripped stratum corneum samples might be useful for the early and precise diagnosis of congenital ichthyoses, including SHCB.

    DOI: 10.1016/j.jlr.2022.100308

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  • A case of <scp>neuro‐Behcet</scp> 's disease preceded by cellulitis‐like cutaneous arteritis and multiple pharyngeal ulcers

    Emi Yokoyama, Yoshio Kawakami, Takayuki Katsuyama, Hidenori Marunaka, Shin Morizane

    International Journal of Dermatology   2022.10

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    Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/ijd.16460

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/ijd.16460

  • Novel homozygous missense mutation c.1654G>T in the ALOX12B gene causing congenital ichthyosiform erythroderma. International journal

    Kotoe Mae, Yoshio Kawakami, Ai Kajita, Takuya Takeichi, Tatsuhiro Noda, Yoji Hirai, Masashi Akiyama, Shin Morizane

    The Journal of dermatology   50 ( 1 )   e37-e38   2022.10

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  • 骨転移,肺転移を来たした基底細胞癌の1例

    浦上 仁志, 加持 達弥, 山崎 修, 山田 潔, 黒住 和彦, 国定 俊之, 田端 雅弘, 森実 真

    西日本皮膚科   84 ( 5 )   443 - 446   2022.10

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    59歳,女性。初診の9年前より前頭部右側に徐々に増大する硬化性局面を認めた。限局性強皮症と考えられ経過観察されていたが,同部位に潰瘍を生じ皮膚生検にて基底細胞癌と診断された。下床の頭蓋骨を含めて広範囲切除し,前外側大腿遊離皮弁による再建術を施行した。病理組織学的所見では斑状強皮症型/破壊型であり,頭蓋骨内部への浸潤が認められた。切除断端陰性であったが,約半年後に第5胸椎左横突起転移を生じ切除術を施行した。その約1年後右大腿骨内側顆に転移が出現し,切除・人工関節置換術後にシスプラチン・ドキソルビシン療法を施行した。その約2年後,右肺門部転移が出現しカルボプラチン・パクリタキセル療法を施行した。その後,特発性器質化肺炎を生じたため化学療法を中止し,プレドニゾロン投与にて加療した。その後は化学療法の再開を希望されなかったため,緩和的放射線療法を施行した。(著者抄録)

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  • 当科で経験した医原性免疫不全関連リンパ増殖性疾患10例の検討

    森実 真, 川上 佳夫, 竹崎 大輝

    西日本皮膚科   84 ( 5 )   469 - 469   2022.10

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  • 全長BP180 ELISA法にて診断し得た水疱性類天疱瘡の1例

    石井 芙美, 三宅 智子, 杉本 佐江子, 川上 佳夫, 松田 真由子, 森田 安理, 山崎 修, 石井 文人, 泉 健太郎, 西江 渉, 橋本 隆, 森実 真

    皮膚科の臨床   64 ( 10 )   1675 - 1679   2022.9

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    <文献概要>80歳,男性。2001年より夏季に下腿に水疱が出現した。病理組織像では好酸球浸潤を混じる表皮下水疱を認め,水疱性類天疱瘡を疑ったが,抗BP180 NC16a部位抗体,蛍光抗体法と全長BP180 ELISAは陰性だった。経過中,蛍光抗体間接法のみ表皮側に陽性となった。2018年に四肢・体幹にびらんと緊満性水疱が生じ,蛍光抗体直接法で表皮基底膜部にIgGが線状に沈着し,全長BP180 ELISAは陽性,免疫ブロット法でBP180 C末端部リコンビナント蛋白と120kDa LAD-1に対する抗体陽性,ELISAでBP230に対する抗体も陽性となり,水疱性類天疱瘡と診断した。症状を繰り返すなかでepitope spreading現象がおきたと考えた。

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  • A Case of Multiple Pyogenic Granulomas Induced by Ramucirumab

    Mana USUI, Emi YOKOYAMA, Hayato NOMURA, Osamu YAMASAKI, Eiki ICHIHARA, Shin MORIZANE

    Nishi Nihon Hifuka   84 ( 4 )   337 - 340   2022.8

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    DOI: 10.2336/nishinihonhifu.84.337

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  • ラムシルマブにより生じた多発性毛細血管拡張性肉芽腫の1例

    臼井 真菜, 横山 恵美, 野村 隼人, 山崎 修, 市原 英基, 森実 真

    西日本皮膚科   84 ( 4 )   337 - 340   2022.8

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    47歳,女性。肺腺扁平上皮癌に対してドセタキセル(DTX)とラムシルマブ(RAM)の併用療法を開始3ヵ月後から,頭頸部・体幹・上肢に2~6mmの紅色丘疹が多発した。増大傾向で出血もみられたため,初診時から3ヵ月の間に合計8ヶ所の病変を切除した。病理組織学的に,いずれも毛細血管の増生がみられ毛細血管拡張性肉芽腫(Pyogenic Granuloma:PG)の診断であった。その後原病の増悪のためRAMは中止となったが,PGの新生はみられず,切除せず残存していたPGはすべて消退した。RAM投与中にPGが生じた報告は自験例を含めて15例報告されており,通常のPGと比較して一部異なる経過もみられた。出血を伴うこともあるため慎重な経過観察が必要であり,症状増悪があれば外科的切除すべきであると考えた。(著者抄録)

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  • Aberrant serine protease activities in atopic dermatitis

    Shin Morizane, Ko Sunagawa, Hayato Nomura, Mamoru Ouchida

    JOURNAL OF DERMATOLOGICAL SCIENCE   107 ( 1 )   2 - 7   2022.7

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    Atopic dermatitis (AD) is a chronic inflammatory skin disease; the three major factors responsible for AD, i.e., epidermal barrier dysfunction, allergic inflammation, and itching, interact with each other to form a pathological condition. Excessive protease activities are characteristic abnormalities that affect the epi-dermal barrier in patients with AD. In normal skin, epidermal serine protease activities are controlled by kallikrein-related peptidases (KLKs) and their inhibitors, including lympho-epithelial Kazal-type-related inhibitor (LEKTI). In AD lesions, KLKs are excessively expressed, which results in the enhancement of epi-dermal serine protease activities and facilitates the invasion by allergens and microorganisms. In addition, some KLKs can activate protease-activated receptor 2 (PAR2) in epidermal keratinocytes and peripheral nerves, resulting in the induction of inflammation and itching. Furthermore, in AD patients with single nucleotide polymorphism (SNP) such as E420K and D386N of SPINK5 which encodes LEKTI, LEKTI function is attenuated, resulting in the activation of KLKs and easy invasion by allergens and microorganisms. Further analysis is needed to elucidate the detailed mechanism underlying the control of serine protease activities, which may lead to the development of new therapeutic and prophylactic agents for AD.(c) 2022 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jdermsci.2022.06.004

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  • 妊娠中の外陰部類上皮肉腫の1例

    藤井 江利子, 山崎 修, 赤松 由規, 川本 友子, 杉原 悟, 森実 真

    日本皮膚外科学会誌   26 ( 1 )   30 - 31   2022.7

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  • Two Cases of Rheumatoid Arthritis with Cuataneous Leukocytoclastic Vasculitis Induced by Tocilizumab

    Mana USUI, Emi YOKOYAMA, Osamu YAMASAKI, Takayuki KATSUYAMA, Miyuki KOSOGABE, Yoshinori SHIRAFUJI, Shin MORIZANE

    Nishi Nihon Hifuka   84 ( 3 )   202 - 206   2022.6

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    DOI: 10.2336/nishinihonhifu.84.202

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  • トシリズマブ投与後に皮膚白血球破砕性血管炎がみられた関節リウマチの2例

    臼井 真菜, 横山 恵美, 山崎 修, 勝山 隆行, 香曽我部 幸, 白藤 宜紀, 森実 真

    西日本皮膚科   84 ( 3 )   202 - 206   2022.6

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    症例1:67歳,女性。X-23年関節リウマチ(RA)を発症した。アバタセプトからトシリズマブ(TCZ)へ変更1ヵ月後,四肢体幹に紅色丘疹や膿疱が出現した。生検では表皮内に好中球浸潤,真皮浅層に白血球破砕性血管炎を認めた。プレドニゾロン(PSL)3mg/日から7.5mg/日へ増量し,ステロイド外用で改善したためTCZを継続したが皮疹は再燃しなかった。症例2:65歳,女性。X-15年RAを発症した。PSL20mg/日,タクロリムス3mg/日併用で症状は改善したが,PSL10mg/日から9mg/日へ減量,インフリキシマブをTCZに変更1ヵ月後に両下腿に小潰瘍が多発し,急激に拡大した。痂疲を伴う紫斑が散在し,最大8cmまでの黄白色壊死組織を付す皮膚潰瘍がみられた。生検では表皮内膿疱,真皮浅層に白血球破砕性血管炎を認めた。TCZを中止し,PSL50mg/日に増量し,シクロホスファミドパルスを6回施行したところ潰瘍は上皮化し治癒した。自験例はいずれもTCZ初回投与後に皮膚症状が出現し,病理組織学的に白血球破砕性血管炎を呈した。TCZは血管炎治療にも用いられるが,今回TCZ投与後に血管炎を伴う皮疹が生じたため,paradoxical reactionの可能性を考えた。文献的にはTCZのparadoxical reaction 10例中4例で投与継続可能であり,症例1でも投与を継続した。TCZはRAに有効な薬剤であり,その副作用としての皮疹には十分注意した上で継続可否の判断をすることが重要である。(著者抄録)

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  • Flare-up of generalized pustular psoriasis following Pfizer-BioNTech BNT162b2 mRNA COVID-19 vaccine: Two cases without mutations of IL36RN and CARD14 genes. International journal

    Kota Tachibana, Yoshio Kawakami, Mayu Tokuda, Shiho Sato, Satoru Sugihara, Tomoko Miyake, Kazumitsu Sugiura, Shin Morizane

    The Journal of dermatology   49 ( 10 )   e393-e394   2022.5

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  • 【(新規)抗腫瘍薬~光と影~】二次感染を契機に皮膚症状が増悪しモガムリズマブが奏効したSezary症候群

    赤松 由規, 平井 陽至, 三宅 智子, 森実 真

    皮膚病診療   44 ( 5 )   410 - 413   2022.5

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    <文献概要>症例のポイント ・二次感染を契機に皮膚症状が増悪したが,モガムリズマブが奏効し長期に寛解維持している.・皮膚T細胞リンパ腫患者において,細菌感染症などの増悪因子除去も重要である.

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  • Obesity and Dyslipidemia Synergistically Exacerbate Psoriatic Skin Inflammation. International journal

    Kenta Ikeda, Shin Morizane, Takahiko Akagi, Sumie Hiramatsu-Asano, Kota Tachibana, Ayano Yahagi, Masanori Iseki, Hideaki Kaneto, Jun Wada, Katsuhiko Ishihara, Yoshitaka Morita, Tomoyuki Mukai

    International journal of molecular sciences   23 ( 8 )   2022.4

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    Patients with psoriasis are frequently complicated with metabolic syndrome; however, it is not fully understood how obesity and dyslipidemia contribute to the pathogenesis of psoriasis. To investigate the mechanisms by which obesity and dyslipidemia exacerbate psoriasis using murine models and neonatal human epidermal keratinocytes (NHEKs), we used wild-type and Apoe-deficient dyslipidemic mice, and administered a high-fat diet for 10 weeks to induce obesity. Imiquimod was applied to the ear for 5 days to induce psoriatic dermatitis. To examine the innate immune responses of NHEKs, we cultured and stimulated NHEKs using IL-17A, TNF-α, palmitic acid, and leptin. We found that obesity and dyslipidemia synergistically aggravated psoriatic dermatitis associated with increased gene expression of pro-inflammatory cytokines and chemokines. Treatment of NHEKs with palmitic acid and leptin amplified pro-inflammatory responses in combination with TNF-α and IL-17A. Additionally, pretreatment with palmitic acid and leptin enhanced IL-17A-mediated c-Jun N-terminal kinase phosphorylation. These results revealed that obesity and dyslipidemia synergistically exacerbate psoriatic skin inflammation, and that metabolic-disorder-associated inflammatory factors, palmitic acid, and leptin augment the activation of epidermal keratinocytes. Our results emphasize that management of concomitant metabolic disorders is essential for preventing disease exacerbation in patients with psoriasis.

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  • Clinical characteristics of Japanese pustular psoriasis: A multicenter observational study

    Chika Ohata, Noriko Tsuruta, Kentaro Yonekura, Yuko Higashi, Kanami Saito, Eri Katayama, Shinichi Imafuku, Chika Ohata, Noriko Tsuruta, Sakae Kaneko, Kotaro Ito, Yuki Matsuzaka, Yuta Koike, Hiroyuki Murota, Takuya Miyagi, Hiroyuki Sakugawa, Kenzo Takahashi, Fusako Okazaki, Kazuki Yamaguchi, Kazunari Sugita, Aki Hashimoto, Maki Kuwashiro, Kentaro Yonekura, Etsuko Okada, Natsuko Sasaki, Yuko Higashi, Masutaka Furue, Takeshi Nakahara, Kenta Ikeda, Shin Morizane, Yutaka Hatano, Kanami Saito, Satoko Kikuchi, Kayo Harada, Eri Katayama, Bungo Ohyama, Shinichi Imafuku

    Journal of Dermatology   49 ( 1 )   142 - 150   2022.1

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    Generalized pustular psoriasis (GPP) is a rare and severe subtype of psoriasis. Because of its rarity, GPP studies with a large sample size have been scarce. We studied the characteristics of GPP and pustular psoriasis using data from the West Japan Psoriasis Registry that had been registered until the end of December 2020. The dataset included 104 patients with pustular psoriasis and 1290 patients with other subtypes of psoriasis. Multivariate analysis revealed a significantly greater number of female patients, a significantly lower mean body mass index, and a significantly lower ratio of habitual drinkers in pustular psoriasis, compared to other subtypes of psoriasis. Of the 104 patients, 102 had GPP, including 88 von Zumbusch, 10 juvenile-onset, and four annular pustular psoriasis. Although the male : female ratio of GPP with psoriasis vulgaris (GPP+PsV) (47/20) was similar to that of psoriasis in Japan, the GPP without PsV (GPP−PsV) group highlighted a female predominance (13/22). The mean age at GPP onset was 45.3 years, and the mean interval from PsV onset to GPP onset was 12.5 years. Four of nine patients with GPP had an IL36RN gene mutation. Infection, medicine, and pregnancy were the precipitating factors for GPP. A family history of psoriasis was present in eight (7.8%) patients with GPP. Twenty-four patients with GPP had psoriatic arthritis. Biologics were used in 76.5% of patients with GPP, followed by etretinate (37.3%), cyclosporine (24.5%), methotrexate (13.7%), apremilast (8.8%), and granulocyte and monocyte adsorption apheresis (6.9%). Etretinate was used in 17 (51.5%) of 33 patients with GPP with less than 10-year history. Thus, etretinate remains a good treatment option for GPP even in the era of biologics. Hypertension was the most commonly identified comorbidity, followed by diabetes. We believe that the characteristics revealed in this study can further contribute to effective GPP management.

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  • Impetigo herpetiformis successfully treated with brodalumab. International journal

    Yumi Nakai, Himino Ashida, Ai Kajita, Emi Yokoyama, Kazumitsu Sugiura, Shin Morizane

    The Journal of dermatology   49 ( 4 )   e149-e150   2021.12

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  • Epithelioid cell granuloma formation in CARD14-associated papulosquamous eruptions. International journal

    Takuya Takeichi, Kenta Ikeda, Yoshinao Muro, Tomoo Ogi, Shin Morizane, Masashi Akiyama

    Journal of the European Academy of Dermatology and Venereology : JEADV   2021.12

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  • A case of bullous pemphigoid after a long‐term administration of anti‐PD‐1 antibodies in a patient with non‐small‐cell lung cancer

    Emi Yokoyama, Osamu Yamasaki, Toshio Kubo, Hiroshi Koga, Norito Ishii, Shin Morizane

    Journal of Cutaneous Immunology and Allergy   2021.12

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    DOI: 10.1002/cia2.12189

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  • Multifaceted Analysis of IL-23A- and/or EBI3-Including Cytokines Produced by Psoriatic Keratinocytes. International journal

    Kota Tachibana, Nina Tang, Hitoshi Urakami, Ai Kajita, Mina Kobashi, Hayato Nomura, Minori Sasakura, Satoru Sugihara, Fan Jiang, Nahoko Tomonobu, Masakiyo Sakaguchi, Mamoru Ouchida, Shin Morizane

    International journal of molecular sciences   22 ( 23 )   2021.11

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    Interleukin (IL) 23 (p19/p40) plays a critical role in the pathogenesis of psoriasis and is upregulated in psoriasis skin lesions. In clinical practice, anti-IL-23Ap19 antibodies are highly effective against psoriasis. IL-39 (p19/ Epstein-Barr virus-induced (EBI) 3), a newly discovered cytokine in 2015, shares the p19 subunit with IL-23. Anti-IL-23Ap19 antibodies may bind to IL-39; also, the cytokine may contribute to the pathogenesis of psoriasis. To investigate IL23Ap19- and/or EBI3-including cytokines in psoriatic keratinocytes, we analyzed IL-23Ap19 and EBI3 expressions in psoriasis skin lesions, using immunohistochemistry and normal human epidermal keratinocytes (NHEKs) stimulated with inflammatory cytokines, using quantitative real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and liquid chromatography-electrospray tandem mass spectrometry (LC-Ms/Ms). Immunohistochemical analysis showed that IL-23Ap19 and EBI3 expressions were upregulated in the psoriasis skin lesions. In vitro, these expressions were synergistically induced by the triple combination of tumor necrosis factor (TNF)-α, IL-17A, and interferon (IFN)-γ, and suppressed by dexamethasone, vitamin D3, and acitretin. In ELISA and LC-Ms/Ms analyses, keratinocyte-derived IL-23Ap19 and EBI3, but not heterodimeric forms, were detected with humanized anti-IL-23Ap19 monoclonal antibodies, tildrakizumab, and anti-EBI3 antibodies, respectively. Psoriatic keratinocytes may express IL-23Ap19 and EBI3 proteins in a monomer or homopolymer, such as homodimer or homotrimer.

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  • Establishment of the Western Japan Psoriasis Registry and first cross-sectional analysis of registered patients

    Noriko Tsuruta, Shinichi Imafuku, Kazuki Yamaguchi, Eri Katayama, Takekuni Nakama, Yuko Higashi, Kentaro Yonekura, Aki Hashimoto, Maki Kuwashiro, Yuta Koike, Hiroyuki Murota, Takuya Miyagi, Kenzo Takahashi, Kayo Harada, Kanami Saito, Yutaka Hatano, Yuki Matsuzaka, Kenta Ikeda, Shin Morizane, Satoko Kikuchi, Masataka Ota, Sakae Kaneko, Takeshi Nakahara, Masutaka Furue, Kotaro Ito, Tetsuji Yanase, Natsuko Sasaki, Etsuko Okada, Hiroshi Saruwatari, Chika Ohata, Fusako Okazaki, Kazunari Sugita, Bungo Ohyama

    Journal of Dermatology   48 ( 11 )   1709 - 1718   2021.11

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    The efficacy and safety of new psoriatic treatments are confirmed in clinical trials, but such clinical trial data are limited by the number and heterogeneity of patients. Furthermore, the prevalence and characteristics of psoriasis differ among racial groups. Therefore, it is important to obtain real-world evidence in specific regions. To identify the optimal systemic treatment for psoriatic patients in Western Japan, we established the Western Japan Psoriasis Registry (WJPR). This registry is led by a neutral physicians’ league associated with university hospitals, general hospitals, and clinics that specialize in treatment of psoriasis. Systemically treated psoriatic patients who provided written informed consent were enrolled, and data were collected on their background information, several patient-reported outcomes, dermatologists’ objective evaluations, and treatment regimens. Patient enrollment began in 2019, and 1394 patients had been recruited by the end of 2020. The prevalence of psoriatic arthritis was 27.2% and that of pustular psoriasis was 7.5%. The mean body mass index was 24.1 kg/m2, and 12% of patients had severe obesity (body mass index ≥30 kg/m2). Major comorbidities were hypertension (35.0%), diabetes (14.1%), and hyperlipidemia (12.2%). Serological data showed that hepatitis B virus surface antigen, anti-hepatitis B virus core antibody, anti-hepatitis C virus antibody, and human T-cell leukemia virus type 1 antibody were detected in 1.1%, 18.0%, 3.1%, and 3.7% of patients, respectively. The most frequently used small-molecule-systemic intervention was apremilast (18.0%), followed by methotrexate (7.7%), etretinate (4.2%), and cyclosporin (3.7%). The most frequently used biologics were interleukin (IL)-17 inhibitors (31.8%), followed by IL-23 inhibitors (including IL-12/23 inhibitors) (26.7%), and tumor necrosis factor inhibitors (11.1%). The WJPR is the first Japanese prospective observational cohort of psoriatic patients. Annual WJPR updates may provide the incidences of comorbidities such as cardiovascular events or onset of arthritis in systemically treated patients, identify rare complications, and identify the optimal treatment regimens for various psoriatic patients.

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  • Case of inherited epidermolysis bullosa simplex with KLHL24 gene mutation in Japan. International journal

    Tomoko Miyake, Ken Natsuga, Takatsune Umayahara, Seiko Naito, Junko Yoshimoto, Akemi Senoo, Han-Tang Wang, Chao-Kai Hsu, Osamu Yamasaki, Shin Morizane

    The Journal of dermatology   49 ( 1 )   e24-e25   2021.10

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  • Persistent local inflammation surrounding cement substance of a tick Amblyomma testudinarium after sufficient removal of its mouthpart. International journal

    Shiho Sato, Yoshio Kawakami, Yohei Yasutomi, Emi Yokoyama, Toshiko Yoshioka, Osamu Yamasaki, Shin Morizane

    The Journal of dermatology   49 ( 1 )   e9-e10   2021.10

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  • Multifunctionality of CD8+ T cells and PD-L1 expression as a biomarker of anti-PD-1 antibody efficacy in advanced melanoma. International journal

    Keiko Manabe, Osamu Yamasaki, Yuki Nakagawa, Tomoko Miyake, Heiichiro Udono, Shin Morizane

    The Journal of dermatology   48 ( 8 )   1186 - 1192   2021.8

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    Anti-programmed cell death protein-1 (PD-1) antibodies have become a standard treatment for advanced melanoma. However, a predictive biomarker for assessing the efficacy of anti-PD-1 antibodies has not been identified. In cancer, CD8+ T cells specific for tumor antigens undergo repeated T-cell receptor stimulation due to the persistence of cancer cells and gradually lose their ability to secrete interleukin 2 (IL-2), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ). We aimed to evaluate multi-cytokine production and immune exhaustion of peripheral CD8+ T cells in melanoma patients treated with anti-PD-1 antibodies. Twenty-four melanoma patients treated with nivolumab were included. Effector cytokine production (IL-2, TNF-α, and IFN-γ) and expression of an exhaustion marker (PD-1) in patients' CD8+ cells were analyzed with flow cytometry. The relationships between parameters such as the neutrophil-to-lymphocyte ratio (NLR) and clinical response to nivolumab were examined. Immunohistochemistry for programmed death-ligand 1 (PD-L1) expression in tumor cells and tumor-infiltrating lymphocytes (TILs) and analysis of their association with clinical response were performed. The clinical response rate to nivolumab was 29%. Regarding TILs, NLR, and several other parameters, no significant difference was found between responders and non-responders. The responder group showed an increase in the percentage of PD-1+ CD8+ /TNF-α+ IFN-γ+ or PD-1+ CD8+ /IFN-γ+ IL-2+ TNF-α+ T cells compared to non-responders. Positivity for PD-L1 expression was significantly higher in the responder group than the non-responder group. In advanced melanoma, the percentage of multifunctional CD8+ PD-1+ T cells and PD-L1 expression in the tumors may be a biomarker for a good response to anti-PD-1 antibody monotherapy.

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  • Analysis of clonality in cutaneous B-cell lymphoma and B-cell pseudolymphoma using skin flow cytometry: Comparison of immunophenotyping and gene rearrangement studies. International journal

    Yuki Nakagawa, Toshihisa Hamada, Takahide Takahashi, Tomoko Miyake, Yoji Hirai, Keiji Iwatsuki, Shin Morizane

    The Journal of dermatology   2021.7

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    To identify clonal neoplastic cells in skin affected by B-cell lymphoma using skin flow cytometry (FCM) techniques, we investigated light-chain restriction using skin FCM with clonality assessed by polymerase chain reaction and light-chain restriction by in situ hybridization (ISH). We retrospectively analyzed 16 cases of B-cell lymphoma with cutaneous involvement: primary cutaneous diffuse large B-cell lymphoma, leg type (pcDLBCL-LT) (n = 7), DLBCL-not otherwise specified (DLBCL-NOS) (n = 6), primary cutaneous follicle center lymphoma (pcFCL) (n = 1), and follicular lymphoma (n = 2), as well as cutaneous B-cell pseudolymphoma (n = 9). Results of skin FCM light-chain restriction analyses were compared with immunoglobulin H (IgH) gene rearrangement and κ/λ ISH findings. Skin FCM detected light-chain restriction in 11 of 14 B-cell lymphoma patients but none of the B-cell pseudolymphoma patients. The sensitivity of skin FCM for distinguishing B-cell lymphoma and B-cell pseudolymphoma was 79%, and the specificity was 100%. Eleven of 13 B-cell lymphoma patients exhibited gene rearrangement (sensitivity 85%), whereas six of seven pseudolymphoma patients were negative (specificity 86%). ISH was positive in three of 16 B-cell lymphoma cases (sensitivity 19%) but none of the B-cell pseudolymphoma cases (specificity 100%). ISH sensitivity was 29% for pcDLBCL-LT, 17% for DLBCL-NOS, and 0% for pcFCL and follicular lymphoma. Skin FCM therefore appears to be more sensitive than ISH in detecting light-chain restriction in DLBCL and follicular lymphoma, and as sensitive as IgH gene rearrangement analysis in detecting clonality. Skin FCM is thus a promising diagnostic tool for identifying monoclonal neoplastic B-cell populations.

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  • Granular C3 dermatosis: Report of a case resembling dermatophytid in association with tinea pedis. International journal

    Yoshio Kawakami, Yumi Aoyama, Kotaro Tada, Kazushi Anzawa, Akiko Nishibu, Takashi Mochizuki, Shin Morizane

    The Journal of dermatology   48 ( 7 )   e320-e321   2021.7

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  • Updated allele frequencies of SERPINB7 founder mutations in Asian patients with Nagashima-type palmoplantar keratosis/keratoderma. International journal

    Yasutoshi Ito, Takuya Takeichi, Kenta Ikeda, Kana Tanahashi, Takenori Yoshikawa, Yuya Murase, Yoshinao Muro, Yoshio Kawakami, Yasuo Nakamura, Kanako Matsuyama, Jun Muto, Naoki Oiso, Shin Morizane, Kazumitsu Sugiura, Yasushi Suga, Mariko Seishima, Akira Kawada, Tomoo Ogi, Masashi Akiyama

    Journal of dermatological science   103 ( 2 )   116 - 119   2021.6

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  • Hypothalamic Mass Detected in Langerhans Cell Histiocytosis.

    Ryosuke Takase, Yasuhiro Nakano, Shin Morizane, Fumio Otsuka

    Internal medicine (Tokyo, Japan)   60 ( 11 )   1795 - 1795   2021.6

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    DOI: 10.2169/internalmedicine.5946-20

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  • Cutaneous adult T-cell leukemia/lymphoma with granulomatous reaction. International journal

    Yuki Nakagawa, Yoshio Kawakami, Tomoko Kawamoto, Tomoko Miyake, Yoji Hirai, Osamu Yamasaki, Shin Morizane

    The Journal of dermatology   48 ( 6 )   e273-e274   2021.6

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  • Staphylococcal enterotoxin B- and lipopolysaccharide-induced toxic shock syndrome in a burn patient. International journal

    Osamu Yamasaki, Satoru Sugihara, Ai Kajita, Emi Yokoyama, Tomoko Miyake, Yoji Hirai, Shin Morizane

    The Journal of dermatology   48 ( 4 )   547 - 550   2021.4

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    Toxic shock syndrome (TSS) is caused by toxic shock syndrome toxin 1 or enterotoxins secreted by Staphylococcus aureus. Lipopolysaccharide (LPS) has also been shown to play a major role in the development of sepsis. Staphylococcal superantigens and LPS operate synergistically in conditioning cytokine release and lethal shock in mice. An 80-year-old woman was admitted because of a 20% mixed-depth flame burn. Despite two excisions and grafts, necrotic ulcers with methicillin-resistant Staphylococcus aureus (MRSA) colonization remained. On the 7th day after the operation, she developed shock with an erythematous rash. Blood examination revealed evidence of disseminated intravascular coagulation, and liver and renal dysfunction. A blood culture revealed a staphylococcal enterotoxin B (SEB)-producing strain of MRSA and Klebsiella pneumoniae. The septic symptoms were prolonged, but the condition gradually improved with extensive treatment. T-cell receptor analysis demonstrated a marked accumulation of SEB-mediated Vβ T cells. Stimulation of peripheral blood mononuclear cells in the recovery phase with SEB and LPS induced additive effects on tumor necrosis factor-α, interferon-γ, and interleukin-6 production. Although the present case did not fulfill the clinical criteria for TSS, the additive effects of SEB and LPS might have caused the severe septic shock.

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  • Two cases of severe congenital hypotrichosis caused by compound heterozygous mutations in the LSS gene. International journal

    Mami Murata, Ryota Hayashi, Yoshio Kawakami, Shin Morizane, Yutaka Shimomura

    The Journal of dermatology   48 ( 3 )   392 - 396   2021.3

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    It has recently been shown that bi-allelic mutations in the lanosterol synthase (LSS) gene, which was originally reported as a causative gene for congenital cataracts, underlie a non-syndromic form of hypotrichosis. Furthermore, it has also been revealed that mutations in the LSS gene can cause syndromic forms of hypotrichosis. To date, however, clear genotype-phenotype correlations have not completely been characterized. In this study, we identified two Japanese patients who had severe congenital hypotrichosis without any other associated findings. Their scalp hairs were extremely short and thin, and were able to be plucked easily. Observation of the plucked hairs showed aberrantly-miniaturized anagen hair follicles. Genetic analysis demonstrated that both patients carried bi-allelic mutations in the LSS gene in a compound heterozygote state. Our findings further underscore the crucial roles of the LSS gene in hair follicle development and hair growth in humans.

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  • A case of allergic contact dermatitis due to 3-glyceryl ascorbate in a skin-lightening lotion. International journal

    Yoshio Kawakami, Takatsune Umayahara, Yoji Hirai, Shin Morizane

    Contact dermatitis   2021.2

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    DOI: 10.1111/cod.13820

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  • Case of tufted angioma treated successfully with tranilast, topical steroids and tacrolimus ointment. International journal

    Yuki Nakagawa, Yoji Hirai, Masatoshi Jinnin, Shin Morizane

    The Journal of dermatology   48 ( 2 )   e84-e85   2021.2

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  • Tattoo skin reaction as a skin manifestation of systemic sarcoidosis. International journal

    Tomoyuki Mukai, Dan Fujiwara, Tomoko Miyake, Shunichi Fujita, Shin Morizane, Yoshitaka Morita

    Modern rheumatology case reports   5 ( 1 )   167 - 171   2021.1

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    A 41-year-old man presented with itching of the skin surrounding his tattoos, blurred vision, fever, general fatigue, and arthralgia. Physical examination revealed skin bulges confined to the tattoo ink lines. Histological analyses of the skin revealed non-caseating granulomas surrounding the tattoo inks. Together with other clinical manifestations including uveitis, lymph nodes swelling, and elevated serum angiotensin-converting enzyme and lysozyme, he was diagnosed with systemic sarcoidosis. The administration of prednisolone alleviated the sarcoidosis-related symptoms, including skin changes. This case illustrates that skin changes on tattoos can be a presenting manifestation of systemic sarcoidosis and that skin biopsy is useful in early diagnosis.

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  • Coexistence of Mycobacterium ulcerans ssp. shinshuense and Mycobacterium avium in a patient with Buruli ulcer-compatible lesions. Reviewed International journal

    Shusaku Fujita, Yoshio Kawakami, Haruka Yamasaki, Satoru Sugihara, Tomoko Miyake, Yuji Miyamoto, Yoshihiko Hoshino, Norihisa Ishii, Osamu Yamasaki, Shin Morizane

    The Journal of dermatology   47 ( 11 )   e400-e401   2020.11

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    DOI: 10.1111/1346-8138.15542

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  • Familial nasal dermoid sinus cysts skipping generations. Reviewed International journal

    Kimiko Nishigaki, Osamu Yamasaki, Yoshio Kawakami, Minori Sasakura, Tomoko Miyake, Aiji Ohtsuka, Junko Kousogabe, Shin Morizane

    The Journal of dermatology   47 ( 10 )   e373-e375 - e375   2020.10

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    DOI: 10.1111/1346-8138.15497

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  • Classical Vohwinkel syndrome with heterozygous p.Asp66His mutation in GJB2 gene: Second Asian case. International journal

    Kenta Ikeda, Takuya Takeichi, Yasutoshi Ito, Yoshio Kawakami, Yuki Nakagawa, Seiko Naito, Osamu Yamasaki, Masashi Akiyama, Shin Morizane

    The Journal of dermatology   47 ( 10 )   e352-e354 - e354   2020.10

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  • iPhone charger-induced chemical burn from overnight contact with sweat: Two cases. Reviewed International journal

    Yuki Nakagawa, Takafumi Uchida, Shin Morizane

    The Journal of dermatology   47 ( 10 )   1187 - 1190   2020.10

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    Smartphones have become essential devices in modern society. The coverage rate of smartphones in 2017 in Japan was 75% according to the Ministry of Internal Affairs and Communications. The iPhone is one of the most well-known smartphone brands. According to the manufacturer of iPhones (Apple), more than 200 million iPhones had been sold worldwide by 2017. These devices are often charged at night-time, especially while being used in bed. There are only three reports of smartphone charger-induced skin damage. We present two new cases of skin ulcers induced by an iPhone charger. The iPhone's "lightning cable" has electrodes outside, and we found that this can present a higher risk of causing a skin injury compared with other types of phone chargers. We also investigated the mechanism of the skin ulcers caused by the iPhone charger. The results indicated that these ulcers were chemical burns rather than an electrical injury or heat-induced burn.

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  • Necrobiosis lipoidicaとの鑑別を要したサルコイドーシスの1例

    川本 友子, 川上 佳夫, 山崎 修, 森実 真, 岩月 啓氏

    日本皮膚科学会雑誌   130 ( 11 )   2393 - 2393   2020.10

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  • Surgical site infection caused by Aeromonas hydrophila presenting as necrotizing soft tissue infection after esophagectomy. Reviewed International journal

    Osamu Yamasaki, Eizo Takahashi, Kazuyo Noda, Nobuhiko Kanaya, Shunsuke Tanabe, Yasuhiro Shirakawa, Shin Morizane

    The Journal of dermatology   47 ( 6 )   673 - 676   2020.6

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    Several virulence factors of Aeromonas such as hemolysin, proteases and lipases have been characterized. The relationship between these virulence factors and disease remains unclear. A 71-year-old man underwent thoracoscopic esophagectomy, lymph node dissection and Roux-en-Y reconstruction for esophageal cancer. On postoperative day 1, redness around the wound on the thoracic abdominal wall gradually enlarged and necrosis became apparent with septic shock. Necrotizing soft tissue infection was suspected and emergency surgical debridement was performed. Blood and wound cultures were positive for Aeromonas hydrophila. The strain was found to have hemolytic activity, proteolytic activity and extremely high elastolytic activity. In addition, the strain actively produced elastolytic metalloprotease, which may contribute to extensive tissue necrosis.

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  • The aim of the measurement of Epstein-Barr virus DNA in hydroa vacciniforme and hypersensitivity to mosquito bites. Reviewed International journal

    Tomoko Miyake, Keiji Iwatsuki, Yoji Hirai, Takenobu Yamamoto, Toshihisa Hamada, Kazuyasu Fujii, Hideaki Imamura, Shin Morizane

    Journal of medical virology   2020.4

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    Epstein-Barr virus (EBV) DNA load in the blood increases in posttransplant lymphoproliferative disorders and chronic active EBV infection. In this report, we analyzed the EBV DNA load in the peripheral blood mononuclear cells (PBMCs) and plasma of patients with hydroa vacciniforme (HV) and/or hypersensitivity to mosquito bites (HMB) to understand the clinical significance of EBV DNA load. All 30 patients showed high DNA loads in the PBMCs over the cut-off level. Of 16 plasma samples, extremely high in two samples obtained from patients with hemophagocytic lymphohistiocytosis (HLH). The amount of cell-free DNA in plasma was correlated to the serum levels of lactate dehydrogenase and inversely correlated to platelet counts. These results indicate that the EBV DNA load in PBMCs can provide one of the diagnostic indicators for HV and HMB and marked elevation of cell-free EBV DNA in plasma might be related to cytolysis such as that observed in HLH.

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  • Atezolizumab-induced lichen planus pemphigoides in a patient with metastatic non-small-cell lung cancer. Reviewed International journal

    Haruka Senoo, Yoshio Kawakami, Emi Yokoyama, Osamu Yamasaki, Shin Morizane

    The Journal of dermatology   47 ( 4 )   e121-e122 - e122   2020.4

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    DOI: 10.1111/1346-8138.15248

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  • Mycobacterium tuberculosis infection in psoriatic patients treated with biologics: Real-world data from 18 Japanese facilities. Reviewed International journal

    Sakae Kaneko, Noriko Tsuruta, Kazuki Yamaguchi, Takuya Miyagi, Kenzo Takahashi, Yuko Higashi, Shin Morizane, Hayato Nomura, Michiya Yamaguchi, Ryosuke Hino, Yu Sawada, Motonobu Nakamura, Bungo Ohyama, Chika Ohata, Kentaro Yonekura, Hiroaki Hayashi, Tetsuji Yanase, Yuki Matsuzaka, Kazunari Sugita, Satoko Kikuchi, Chikage Mitoma, Takeshi Nakahara, Masutaka Furue, Fusako Okazaki, Yuta Koike, Shinichi Imafuku

    The Journal of dermatology   47 ( 2 )   128 - 132   2020.2

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    Although rare, tuberculosis has been reported with biologic treatment against psoriasis in Japan, a tuberculosis medium-burden country. Mycobacterial infection often develops after a long incubation period and might not have been adequately identified in clinical trials or post-marketing surveillance. To determine the real-world incidence of tuberculosis in psoriatic patients treated with biologics, we conducted a retrospective, multicenter, observational study in 18 facilities in Western Japan. Psoriatic patients who visited a participating facility between 2010 and March 2017 and received biologic reagents were enrolled. Information on sex, age at first biologic treatment, results of interferon-γ release assay (IGRA) for Mycobacterium tuberculosis, treatment history with isoniazid, and onset of active and/or latent tuberculosis was collected. A total of 1117 patients (830 men and 287 women) were enrolled. The mean duration of biologic treatment was 3.54 years. Sixty-five patients (5.8%) showed positive IGRA results at screening. Active tuberculosis developed in two patients after the administration of tumor necrosis factor inhibitors (both involved miliary tuberculosis). Latent tuberculosis was observed in two patients treated with anti-interleukin-12/23p40 antibody. The incidence rate of tuberculosis, including latent tuberculosis, in this survey was 0.36%. Although the incidence rate of tuberculosis was low considering the observation period of biologic treatment, active tuberculosis was found in both the screening-negative group and a screening-positive subject after isoniazid prophylaxis (both miliary tuberculosis), concluding that negative screening or isoniazid treatment does not always assure that an individual has no tuberculosis. Hence, dermatologists still need to pay careful attention to tuberculosis at every patient visit.

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  • Multifaceted Analyses of Epidermal Serine Protease Activity in Patients with Atopic Dermatitis. Reviewed International journal

    Hayato Nomura, Mutsumi Suganuma, Takuya Takeichi, Michihiro Kono, Yuki Isokane, Ko Sunagawa, Mina Kobashi, Satoru Sugihara, Ai Kajita, Tomoko Miyake, Yoji Hirai, Osamu Yamasaki, Masashi Akiyama, Shin Morizane

    International journal of molecular sciences   21 ( 3 )   2020.1

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    The serine proteases kallikrein-related peptidase (KLK) 5 and KLK7 cleave cell adhesion molecules in the epidermis. Aberrant epidermal serine protease activity is thought to play an important role in the pathogenesis of atopic dermatitis (AD). We collected the stratum corneum (SC) from healthy individuals (n = 46) and AD patients (n = 63) by tape stripping and then measuring the trypsin- and chymotrypsin-like serine protease activity. We also analyzed the p.D386N and p.E420K of SPINK5 variants and loss-of-function mutations of FLG in the AD patients. The serine protease activity in the SC was increased not only in AD lesions but also in non-lesions of AD patients. We found, generally, that there was a positive correlation between the serine protease activity in the SC and the total serum immunoglobulin E (IgE) levels, serum thymus and activation-regulated chemokine (TARC) levels, and peripheral blood eosinophil counts. Moreover, the p.D386N or p.E420K in SPINK5 and FLG mutations were not significantly associated with the SC's serine protease activity. Epidermal serine protease activity was increased even in non-lesions of AD patients. Such activity was found to correlate with a number of biomarkers of AD. Further investigations of serine proteases might provide new treatments and prophylaxis for AD.

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  • Simultaneous development of IgA vasculitis and eosinophilic granulomatosis with polyangiitis. International journal

    Yosuke Asano, Yoshinori Matsumoto, Tatsuhiko Miyazaki, Akihiro Ishizu, Shin Morizane, Keigo Hayashi, Yuriko Yamamura, Sumie Hiramatsu, Yoshia Miyawaki, Michiko Morishita, Keiji Ohashi, Haruki Watanabe, Katsue Sunahori Watanabe, Tomoko Kawabata, Ken-Ei Sada, Hirofumi Makino, Jun Wada

    Modern rheumatology case reports   4 ( 1 )   63 - 69   2020.1

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    Immunoglobulin A (IgA) vasculitis (IgAV) is a small vessel vasculitis presenting cutaneous purpura, arthralgias and/or arthritis, acute enteritis and glomerulonephritis caused by deposition of the IgA1-mediated immune complex. Eosinophilic granulomatosis with polyangiitis (EGPA) is an anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) characterised by eosinophil-rich and granulomatous inflammation in small to medium-sized vessels. Both IgAV and EGPA are classified as autoimmune systemic vasculitis, but the pathogenesis of immune complex-mediated IgAV and that of pauci-immune EGPA are different. Here we report a rare case of simultaneous development of IgAV and EGPA presenting palpable purpura and numbness in a patient with a history of asthma. Histological examination revealed leukocytoclastic vasculitis with deposition of IgA, IgM and C3 in the upper dermis and necrotising vasculitis with eosinophilic infiltration and granulomatous formation in the lower dermis and subcutaneous fat, indicating the existence of IgAV and EGPA. Our case provides evidence of concurrent development of two different types of vasculitis, which may affect disease-associated complications, therapeutic strategy and prognosis.

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  • Serum 5-S-cysteinyl-dopa levels as a predictive marker for the efficacy of nivolumab in advanced malignant melanoma. Reviewed International journal

    Hiroshi Umemura, Tatsuya Kaji, Kota Tachibana, Shin Morizane, Osamu Yamasaki

    The International journal of biological markers   34 ( 4 )   414 - 420   2019.12

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    PURPOSE: With the recent developments in novel molecular targeted therapy such as immune-checkpoint blockades, serine/threonine-protein kinase B-Raf, and mitogen-activated protein kinase kinase inhibitors, the prognosis of advanced malignant melanoma has been improving. 5-S-cysteinyl-dopa (5-S-CD), a precursor of pheomelanin, has been previously revealed to be a useful biomarker for advanced-stage malignant melanoma, especially in patients with distant metastases. Here, we aimed to assess and compare the utility of serum 5-S-CD and lactate dehydrogenase levels as markers for predicting the effects of nivolumab in advanced malignant melanoma. METHODS: Baseline serum 5-S-CD and lactate dehydrogenase levels in patients with unresectable stage IIIC and IV malignant melanoma treated with nivolumab (n = 21) were analyzed to determine their utility as predictive markers for survival. We also analyzed the prognostic value of these markers among patients with only stage IV malignant melanoma (n = 17). RESULTS: Our analysis showed that patients with baseline serum 5-S-CD levels >25.0 nmol/L had significantly poor prognosis. In contrast, serum lactate dehydrogenase levels at the upper limit of the normal range did not exhibit such changes. CONCLUSIONS: Serum 5-S-CD levels have the potential to be an excellent predictive marker for the efficacy of nivolumab therapy in patients with advanced malignant melanoma.

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  • The expression of cell adhesion molecule 1 and its splicing variants in Sézary cells and cell lines from cutaneous T-cell lymphoma. Reviewed International journal

    Mari Yamaguchi, Shin Morizane, Toshihisa Hamada, Tomoko Miyake, Makoto Sugaya, Hiroaki Iwata, Kazuyasu Fujii, Rie Haramoto-Shiratsuki, Yuki Nakagawa, Mayumi Miura, Koichi Ohshima, Kazuhiro Morishita, Takahide Takahashi, Masahide Imada, Ken Okada, Jiro Uehara, Junko Sowa-Osako, Keiji Iwatsuki

    The Journal of dermatology   46 ( 11 )   967 - 977   2019.11

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    Cell adhesion molecule 1 (CADM1) is aberrantly expressed by T-cell neoplasms such as adult T-cell leukemia/lymphoma (ATLL) and mycosis fungoides (MF). We studied the expression of CADM1 and its splicing variants in Sézary syndrome (SS), MF, other cutaneous T-cell lymphoma (CTCL), and cell lines derived from T- and B-cell lymphomas. Soluble CADM1 was measured in the patients' sera. CADM1+ cells in the blood and skin lesions were examined by flow cytometry and immunostaining, respectively. Soluble CADM1 was measured by ELISA, and the splicing variants of CADM1 transcripts were determined by reverse transcriptase-polymerase chain reaction, followed by sequencing. As a result, circulating CADM1+ cells were significantly increased in seven out of 10 patients with SS, ranging from 7.9% to 74.5% of the CD3+CD4+ fractions (median 33.7%; cut-off value 6.5%). The percentages of CADM1+ cells were usually less than those of circulating Sézary cells. CADM1 was expressed, to various degrees, in six of nine T-cell lines derived from SS, MF, ATLL, and anaplastic large cell lymphoma (ALCL), but negative in B-cell lymphoma-derived cell lines. CADM1+ cells were present in the skin infiltrates of MF, SS, ATLL and ALCL. Serum levels of soluble CADM1 were not significantly elevated in SS/MF. Three major splicing variants of CADM1 expressed by neoplastic T-cells contained different combinations of the exons 7, 8, 9 and 11, including a putative oncogenic variant composed of exons 7-8-9-11. In conclusion, CADM1 is frequently expressed in Sézary cells and cell lines from CTCL.

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  • Single institutional experience of radiation therapy for angiosarcoma of the scalp without cervical lymph node metastases: Impact of concurrent chemoradiation with maintenance chemotherapy using taxanes on patient prognosis. Reviewed International journal

    Hiroki Ihara, Tatsuya Kaji, Kuniaki Katsui, Tomoko Miyake, Takahiro Waki, Norihisa Katayama, Hidenobu Matsuzaki, Osamu Yamasaki, Masahiro Kuroda, Shin Morizane, Susumu Kanazawa

    Molecular and clinical oncology   11 ( 5 )   498 - 504   2019.11

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    Cutaneous angiosarcoma is a rare aggressive malignant tumor. Concurrent chemoradiation (CCRT) with maintenance chemotherapy using taxanes is one of the primary treatments. The aim of the present study was to retrospectively analyze the efficacy of CCRT with maintenance chemotherapy using taxanes in localized angiosarcoma of the scalp without cervical lymph node metastases. A total of 19 patients treated with radiation therapy for localized angiosarcomas of the scalp without cervical lymph node metastases were enrolled. The overall survival (OS), progression-free survival (PFS), and local control (LC) rates were calculated using Kaplan-Meier analysis. Univariate analyses were performed for various potential prognostic factors for OS, PFS, and LC. The median radiation dose was 70 Gy (range, 60-70 Gy), and the fractional dose was 2 Gy. Radiation therapy alone, radiation therapy + interleukin-2, surgery + CCRT with maintenance chemotherapy, CCRT with maintenance chemotherapy, and CCRT without maintenance chemotherapy were administered to 2, 4, 2, 9 and 2 patients, respectively. The 1- and 3-year OS, PFS, and LC rates were 88 and 52%, 47 and 33%, and 74 and 56%, respectively. CCRT with maintenance chemotherapy and surgery were significant prognostic factors for PFS (P=0.036 and 0.025, respectively). Therefore, CCRT with maintenance chemotherapy using taxanes might be effective in treating localized angiosarcomas of the scalp without cervical lymph node metastases.

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  • 治療抵抗性リンパ増殖性好酸球増多症候群の1例 Reviewed

    加藤 あずさ, 三宅 智子, 濱田 利久, 花山 宜久, 森実 真, 岩月 啓氏

    臨床皮膚科   73 ( 12 )   983 - 989   2019.11

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  • Pediatric-onset annular pustular psoriasis in a patient with Down syndrome. Reviewed International journal

    Anri Morita, Yoshio Kawakami, Tatsuya Kaji, Yoji Hirai, Tomoko Miyake, Masayuki Takahashi, Osamu Yamasaki, Kazumitsu Sugiura, Shin Morizane

    The Journal of dermatology   46 ( 10 )   e367-e368   2019.10

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  • TNF-α and IL-17A induce the expression of lympho-epithelial Kazal-type inhibitor in epidermal keratinocytes. Reviewed International journal

    Satoru Sugihara, Saeko Sugimoto, Kota Tachibana, Mina Kobashi, Hayato Nomura, Tomoko Miyake, Yoji Hirai, Osamu Yamasaki, Shin Morizane

    Journal of dermatological science   96 ( 1 )   26 - 32   2019.10

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    BACKGROUND: Serine proteases have important roles in skin barrier function and desquamation, and the aberrant expression or the dysfunction of serine proteases is associated with the pathogenesis of skin diseases. Serine protease activities are tightly regulated by serine proteases such as kallikrein-related peptidases (KLKs) and serine protease inhibitors such as lympho-epithelial Kazal-type related inhibitor (LEKTI). For a better understating of diseases' pathogenesis, the regulation mechanism of serine proteases and the inhibitors' expression in epidermal keratinocytes must be clarified. OBJECTIVES: To investigate the effects of the cytokines on the expression of LEKTI in epidermal keratinocytes. METHODS: Normal human epidermal keratinocytes (NHEKs) were stimulated with panels of inflammatory cytokines. The expression of serine protease inhibitors was analyzed using quantitative real-time PCR and ELISA. LEKTI expression in normal human skin and lesions from psoriasis or atopic dermatitis (AD) were analyzed by immunohistochemically and tape-stripping. Trypsin- and chymotrypsin-like serine protease activities in culture supernatants were measured by using specific substrates. RESULTS: TNF-α and IL-17A significantly induced the expression of LEKTI in NHEKs. The immunohistochemical and tape-stripping analysis revealed that psoriatic skin lesions had higher LEKTI expression compared to normal skin and AD lesions. Trypsin- and chymotrypsin-like protease activities in the culture media were upregulated 3-5 days later but attenuated 6-7 days later period by these cytokines. CONCLUSIONS: In epidermal keratinocytes, the Th1&Th17 cytokines TNF-α and IL-17A induce the expression of serine protease inhibitor LEKTI, and it might occur to suppress the increase in the serine protease activities under inflammation.

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  • Clinical manifestations of skin, lung and muscle diseases in dermatomyositis positive for anti-aminoacyl tRNA synthetase antibodies. Reviewed International journal

    Hiroko Fukamatsu, Yoji Hirai, Tomoko Miyake, Tatsuya Kaji, Shin Morizane, Emi Yokoyama, Toshihisa Hamada, Takashi Oono, Yoshinobu Koyama, Seiichiro Norikane, Keiji Iwatsuki

    The Journal of dermatology   46 ( 10 )   886 - 897   2019.10

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    Patients with dermatomyositis positive for anti-aminoacyl tRNA synthetase (ARS) antibodies, also known as antisynthetase syndrome (ASS), frequently present with mechanic's hand and interstitial lung disease (ILD). We first screened the antibody profiles of 59 patients with dermatomyositis, and then examined the cutaneous, muscular and pulmonary manifestations characteristic for patients with ASS. The anti-ARS antibodies Jo-1, PL-7, PL-12, EJ and KS, along with antibodies to TIF1-γ, MDA5 and Mi-2, were examined. Among the 59 patients, 20, 21, 15 and three patients were classified into the ASS, non-ASS, myositis-specific antibody-negative and unknown groups, respectively. Five of 16 patients (31%) with ASS had six relatives with a history of collagen diseases, within the second degree of relationship, including two cases of dermatomyositis (vs the non-ASS group, P = 0.018). Patients with ASS more frequently presented with fever and arthralgia, and had elevated levels of C-reactive protein. Nine of the 11 finger lesions (82%) clinically diagnosed as mechanic's hands showed a psoriasiform tissue reaction. ILD was observed in 19 of 20 patients (95%) with ASS, and eight of 21 patients (38%) in the non-ASS group, in which six patients possessed anti-MDA5 antibody. Patients with ASS showed higher serum levels of muscle enzymes, and four of 12 patients (33%) had fasciitis-dominant myopathy, while only one of 11 patients (9%) in the non-ASS group had fasciitis-dominant myopathy. Patients with ASS often present with a psoriasiform tissue reaction in the hand lesions and fasciitis-dominant myopathy, and the relatives of those with ASS are at high risk for collagen diseases.

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  • Clinical manifestations of skin, lung and muscle diseases in dermatomyositis positive for anti-aminoacyl tRNA synthetase antibodies. Reviewed

    Fukamatsu H, Hirai Y, Miyake T, Kaji T, Morizane S, Yokoyama E, Hamada T, Oono T, Koyama Y, Norikane S, Iwatsuki K.

    The Journal of Dermatology   2019.8

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  • 瀬戸内海に生息するナルトビエイによる外傷後に生じたMycobacterium marinum感染症の1例

    谷本 尚吾, 川上 佳夫, 安富 陽平, 山崎 修, 森実 真

    日本皮膚科学会雑誌   129 ( 8 )   1652 - 1652   2019.7

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  • Two cases of metallic foreign bodies hidden in recalcitrant wounds unexpectedly detected using magnetic resonance imaging. Reviewed International journal

    Kota Tachibana, Hiroshi Umemura, Shin Morizane, Osamu Yamasaki

    The Journal of dermatology   46 ( 6 )   e195-e196 - e196   2019.6

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    DOI: 10.1111/1346-8138.14756

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  • 有茎広背筋皮弁で加療した冠動脈ステント留置後の右肩甲部放射線性皮膚潰瘍の1例

    森田 安理, 山崎 修, 石井 芙美, 川上 佳夫, 加持 達弥, 竹原 彩, 大塚 正樹, 森実 真

    西日本皮膚科   81 ( 3 )   242 - 242   2019.6

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  • ハンセン病LL型新規発症の1例

    篠倉 美理, 川上 佳夫, 杉本 佐江子, 山崎 修, 森実 真, 吉原 由樹, 多田 譲治, 尾崎 元昭, 石井 則久

    日本皮膚科学会雑誌   129 ( 4 )   602 - 602   2019.4

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  • Cross-sectional multicenter observational study of psoriatic arthritis in Japanese patients: Relationship between skin and joint symptoms and results of treatment with tumor necrosis factor-α inhibitors. Reviewed

    Tsuruta N, Narisawa Y, Imafuku S, Ito K, Yamaguchi K, Miyagi T, Takahashi K, Fukamatsu H, Morizane S, Koketsu H, Yamaguchi M, Hino R, Nakamura M, Ohyama B, Ohata C, Kuwashiro M, Sato T, Saito K, Kaneko S, Yonekura K, Hayashi H, Yanase T, Morimoto K, Sugita K, Yanagihara S, Kikuchi S, Mitoma C, Nakahara T, Furue M, Okazaki F.

    The Journal of Dermatology   2019.3

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  • An effective and promising treatment with adalimumab for impetigo herpetiformis with postpartum flare-up. Reviewed International journal

    Tamayo Yamashita, Toshihisa Hamada, Yuka Maruta, Ai Kajita, Yoji Hirai, Shin Morizane, Soichiro Watanabe, Kazumitsu Sugiura, Keiji Iwatsuki

    International journal of dermatology   58 ( 3 )   350 - 353   2019.3

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    DOI: 10.1111/ijd.14141

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  • Cetuximab投与中に生じたleukocytoclastic vasculitis

    安富 陽平, 篠倉 美理, 神野 泰輔, 藤本 裕子, 川上 佳夫, 山崎 修, 森実 真

    日本皮膚科学会雑誌   129 ( 3 )   395 - 395   2019.3

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  • The Role of Kallikrein-Related Peptidases in Atopic Dermatitis. Reviewed

    Shin Morizane

    Acta medica Okayama   73 ( 1 )   1 - 6   2019.2

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    Excessive protease activity is a characteristic abnormality that affects the epidermal barrier in patients with atopic dermatitis (AD). Kallikrein-related peptidases (KLKs) are excessively expressed in AD lesions, and it is suggested that the abnormal action of KLKs is involved in the skin barrier dysfunction in AD. In other words, overexpressed KLKs disrupt the normal barrier function, and due to that breakdown, external substances that can become antigens of AD easily invade the epidermis, resulting in dermatitis, coupled with the induction of Th2 cytokines. Further investigations are required to elucidate the role of KLKs in AD; this knowledge could contribute to the design of new therapeutic and prophylactic drugs for AD.

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  • Autophagy in malnutrition-associated dermatoses. Reviewed

    Hirai Y, Miyake T, Hamada T, Yamasaki O, Morizane S, Mori T, Iwatsuki K.

    The Journal of Dermatology   46 ( 1 )   43 - 47   2019.1

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    DOI: 10.1111/1346-8138.14694.

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  • Two cases of metallic foreign bodies hidden in recalcitrant wounds unexpectedly detected using magnetic resonance imaging. Reviewed

    Tachibana K, Umemura H, Morizane S, Yamasaki O

    The Journal of Dermatology   2019.1

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    DOI: 10.1111/1346-8138.14756.

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  • A case of malignant melanoma associated with polymyositis treated with immune checkpoint inhibitors Reviewed

    Nomura, H., Yamasaki, O., Kaji, T., Wakabayashi, H., Miyawaki, Y., Morizane, S.

    Nishinihon Journal of Dermatology   81 ( 5 )   396 - 400   2019.1

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    DOI: 10.2336/nishinihonhifu.81.396

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  • Autophagy in malnutrition-associated dermatoses. Reviewed International journal

    Yoji Hirai, Tomoko Miyake, Toshihisa Hamada, Osamu Yamasaki, Shin Morizane, Tatsuhiko Mori, Keiji Iwatsuki

    The Journal of dermatology   46 ( 1 )   43 - 47   2019.1

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    Malnutrition-associated dermatoses including necrolytic migratory erythema (NME) and pellagra share common clinicopathological features; in particular, necrolytic changes in the upper epidermis. Here, we report the involvement of autophagy in the development of necrolysis in three patients with malnutrition-associated dermatoses. First, we examined an autophagy-specific molecule, microtubule-associated protein light chain 3 (LC3), using a monoclonal antibody. LC3 was strongly expressed in the granular layers of the active border, and less intensely observed in the perilesional areas. Little LC3 staining or only background levels were observed in control skin diseases including atopic dermatitis (n = 4), psoriasis vulgaris (n = 3), basal cell carcinoma with amyloid deposits (n = 3) and squamous cell carcinoma (n = 3). Electron microscopic observations revealed the presence of autophagosome-like structures in the necrolytic areas. No apoptotic signals were observed in the necrolytic lesion using the terminal deoxynucleotidyl transferase dUTP nick end labeling method. Epidermal Langerhans cells determined by anti-CD1a antibody were markedly decreased in number. Our observations suggest the possibility that malnutrition-associated necrolysis, as exemplified by NME and pellagra, may be induced by autophagy.

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  • Toll-like receptor signalling induces the expression of serum amyloid A in epidermal keratinocytes and dermal fibroblasts. International journal

    S Morizane, A Kajita, K Mizuno, T Takiguchi, K Iwatsuki

    Clinical and experimental dermatology   44 ( 1 )   40 - 46   2019.1

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    BACKGROUND: Toll-like receptors (TLRs) play critical roles in innate immune response by sensing pathogen- or damage-associated molecular patterns. Epidermal keratinocytes and dermal fibroblasts also produce proinflammatory cytokines and chemokines under stimulation with TLR ligands. Serum amyloid A (SAA) is an essential factor in the pathogenesis of secondary amyloidosis, and also has immunomodulatory functions. SAA are produced mainly by hepatocytes but also by a variety of cells, including immune cells, endothelial cells, synoviocytes, and epidermal keratinocytes. However, SAA expression in human dermal fibroblasts has not been shown to date. AIM: To investigate the effect of TLR ligands on SAA expression in epidermal keratinocytes and dermal fibroblasts. METHODS: We investigated whether TLR ligands induce the expression of SAA in normal human epidermal keratinocytes (NHEKs) and normal human dermal fibroblasts (NHDFs) by real-time quantitative PCR and ELISA. The effect of SAA on its own expression in NHDFs was also studied. RESULTS: SAA expression was induced via nuclear factor-κB by TLR1/2, 3, 5 and 2/6 ligands in NHEKs. In NHDFs, TLR1/2 and TLR2/6 ligands increased SAA expression. SAA further induced its own expression via TLR1/2 and NF-κB in NHDFs, as previously reported for NHEKs. CONCLUSIONS: Our results provide new evidence that the skin's innate immune response contributes to the production of SAA, which might lead to an increased risk of systemic complications such as secondary amyloidosis of recessive dystrophic epidermolysis bullosa.

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  • Papuloerythroderma-like cutaneous involvement of a CD62L- subclone of T-cell prolymphocytic leukemia. Reviewed International journal

    Yuki Nakagawa, Toshihisa Hamada, Mayuko Matsuda, Taisuke Kanno, Takumi Kondo, Takahide Takahashi, Toshiyuki Watanabe, Ken Okada, Toru Kawakami, Tomoko Miyake, Shin Morizane, Keiji Iwatsuki

    The Journal of dermatology   46 ( 1 )   65 - 69   2019.1

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    We report the case of an 88-year-old Japanese man with erythrodermic involvement of T-cell prolymphocytic leukemia (T-PLL). He had a history of pharyngeal diffuse large B-cell lymphoma successfully treated with polychemotherapy including cyclophosphamide and epirubicin, 6 years before the current illness. He presented with numerous reddish, coalescing, flat-topped papules on the trunk and extremities, sparing the skin folds of the abdomen, the features of which mimicked those of papuloerythroderma. Immunohistochemistry showed perivascular and epidermotropic infiltration of CD3+ CD4+ T cells in the cutaneous lesion. However, flow cytometric analysis revealed that the skin infiltrating T cells were negative for surface CD4, and that CD3+ CD4- CD8- cells made up 92% of the T-cell fraction of peripheral blood. The circulating atypical T cells had a round or oval nucleus and prominent nucleoli, and the deletion of chromosomes 6q, 13 and 17. These cytological profiles were consistent with those of T-PLL and distinct from those of Sézary cells. The same T-cell clone was detected in the cutaneous lesion and peripheral blood, but the expression of CD62L was absent in the skin infiltrates and present in the circulating cells. No specific mutation was detected in STAT3 or STAT5B. Although low-dose oral etoposide had a beneficial effect on the skin rash, a fatal crisis of marked leukocytosis (169 × 103 /μL) occurred 19 months after the illness onset. CD62L-leukemic cells of T-PLL may infiltrate the skin to form papuloerythroderma-like cutaneous lesions.

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  • Toll-like receptor signaling induces the expression of lympho-epithelial Kazal-type inhibitor in epidermal keratinocytes. Invited Reviewed International journal

    Saeko Sugimoto, Shin Morizane, Hayato Nomura, Mina Kobashi, Satoru Sugihara, Keiji Iwatsuki

    Journal of dermatological science   92 ( 2 )   181 - 187   2018.11

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    BACKGROUND: Lympho-epithelial Kazal-type inhibitor (LEKTI) tightly controls the activities of serine proteases such as kallikrein-related peptidase (KLK) 5 and KLK7 in the epidermis. LEKTI is known to be an essential molecule for the epidermal skin barrier, as demonstrated by SPINK5 nonsense mutation, which results in Netherton syndrome. Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns or damage-associated molecular patterns and produce inflammatory cytokines, chemokines, and antimicrobial peptides. However, the effect of TLR signaling on the expression of LEKTI is not clear. OBJECTIVE: To investigate whether TLR signaling can affect expression of LEKTI in epidermal keratinocytes. METHODS: We stimulated a panel of TLR ligands and investigated the expression of LEKTI in normal human epidermal keratinocytes (NHEKs). We further measured trypsin or chymotrypsin-like serine protease activity in NHEK cultured media under stimulation with TLR3 ligand, poly (I:C). Immunostaining for LEKTI was performed using skin samples from skin infectious diseases. RESULTS: TLR1/2, 3, 5, and 2/6 ligands induced the expression of LEKTI in NHEKs. The trypsin or chymotrypsin-like serine protease activity in NHEKs was up-regulated with the stimulation of poly (I:C). The gene expressions of KLK6, KLK10, KLK11, and KLK13 were also increased by poly (I:C). An immunohistochemical analysis demonstrated that the expression of LEKTI was up-regulated in the lesions of varicella, pyoderma, and rosacea. CONCLUSIONS: TLR signaling induces the expression of LEKTI in epidermal keratinocytes, which might contribute to the control of aberrant serine protease activities in inflammatory skin diseases.

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  • Clinical and Laboratory Markers Associated With Relapse in Cutaneous Polyarteritis Nodosa. International journal

    Azusa Kato, Toshihisa Hamada, Tomoko Miyake, Shin Morizane, Yoji Hirai, Osamu Yamasaki, Keiji Iwatsuki

    JAMA dermatology   154 ( 8 )   922 - 926   2018.8

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    Importance: In cutaneous polyarteritis nodosa (CPAN), less aggressive treatments can be selected, because CPAN is not associated with life-threatening or progressive outcomes. Although patients with a recurrent clinical course may require additional immunosuppressive therapies, no pretreatment factors associated with a worse prognosis in CPAN have been reported. Objective: To identify clinical or laboratory markers associated with relapse of CPAN. Design, Setting, and Participants: This retrospective case series was performed at a dermatology clinic of a tertiary referral center in Okayama, Japan, from October 1, 2001, through April 30, 2017. Of 30 patients identified with CPAN, the 21 with histopathologic evidence of disease were eligible and enrolled in the study. Main Outcomes and Measures: The medical database was examined for sex, age at diagnosis, affected anatomical sites, type and extent of skin lesion, laboratory data, initial therapies, duration of follow-up, and current status. Relapse was defined as the first reoccurrence or new onset of cutaneous disease that required further escalation of treatment with prednisolone at a dosage of greater than 20 mg/d and/or add-on use of immunosuppressant therapy, more than 6 months after initial treatment. The pretreatment factors were statistically evaluated between the groups without and with relapse. Results: The 21 patients included 5 males and 16 females with a median age of 49 years (range, 11-74 years) at diagnosis. The median follow-up was 42 months (range, 8-374 months). Pretreatment cutaneous ulcer was significantly associated with relapse between the 2 groups (0 of 11 in the nonrelapse group vs 4 of 10 in the relapse group; χ21 = 4.67; P < .05). In the laboratory test results, significantly higher mean (SD) values were observed in the relapse group for C-reactive protein level (0.23 [2.00] vs 6.03 [3.10] mg/dL; standard error of the mean [SEM], 3.40 mg/dL; 95% CI, 0.01-10.8 mg/dL; P = .01), absolute neutrophil count (ANC) (3.4 × 103/μL [1.1 × 103/μL] vs 6.0 × 103/μL [3.2 × 103/μL]; SEM, 2.9 × 103/μL; 95% CI, 1.9 × 103/μL to 14.6 × 103/μL; P = .001), neutrophil-to-lymphocyte ratio (1.4 [0.8] vs 2.8 [0.9]; SEM, 1.2; 95% CI, 1.1-4.9; P = .002), and systemic immune-inflammation index (5.1 × 105 [3.9 × 105] vs 11.7 × 105 [7.7 × 105]; SEM, 7.3 × 105; 95% CI, 3.3 × 105 to 31.1 × 105; P = .007). The estimated 2-year cumulative relapse rate was significantly high in the patients with blood ANC of greater than 4.9 × 103/μL compared with 4.9 × 103/μL or less (9 of 10 [90%] vs 2 of 11 [18%]; 95% CI, 6%-72%). Conclusions and Relevance: Pretreatment status of cutaneous ulcer, the serum C-reactive protein level, the blood ANC, the neutrophil-to-lymphocyte ratio, and the systemic immune-inflammation index are associated with a worse prognosis in CPAN.

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  • The synergistic activities of the combination of tumour necrosis factor-α, interleukin-17A and interferon-γ in epidermal keratinocytes. International journal

    S Morizane, H Nomura, K Tachibana, Y Nakagawa, K Iwatsuki

    The British journal of dermatology   179 ( 2 )   496 - 498   2018.8

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    DOI: 10.1111/bjd.16443

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  • Speckled lentiginous naevus: understanding the process of development and regression. International journal

    K Iwatsuki, T Hamada, O Yamasaki, S Morizane, M Muto

    The British journal of dermatology   178 ( 6 )   1447 - 1448   2018.6

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    DOI: 10.1111/bjd.16319

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  • Analysis of All 34 Exons of the SPINK5 Gene in Japanese Atopic Dermatitis Patients.

    Shin Morizane, Mamoru Ouchida, Ko Sunagawa, Saeko Sugimoto, Mina Kobashi, Satoru Sugihara, Hayato Nomura, Kazuhide Tsuji, Atsushi Sato, Yoshihiro Miura, Hiroaki Hattori, Kotaro Tada, Wook-Kang Huh, Akemi Seno, Keiji Iwatsuki

    Acta medica Okayama   72 ( 3 )   275 - 282   2018.6

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    Lympho-epithelial Kazal-type-related inhibitor (LEKTI) is a large multidomain serine protease inhibitor that is expressed in epidermal keratinocytes. Nonsense mutations of the SPINK5 gene, which codes for LEKTI, cause Netherton syndrome, which is characterized by hair abnormality, ichthyosis, and atopy. A single nucleotide polymorphism (SNP) of SPINK5, p.K420E, is reported to be associated with the pathogenesis of atopic dermatitis (AD). We studied all 34 exons of the SPINK5 gene in Japanese 57 AD patients and 50 normal healthy controls. We detected nine nonsynonymous variants, including p.K420E; these variants had already been registered in the SNP database. Among them, p.R654H (n=1) was found as a heterozygous mutation in the AD patients, but not in the control. No new mutation was detected. We next compared the data of the AD patients with data from the Human Genetic Variation Database provided by Kyoto University; a significant difference was found in the frequency of the p.S368N genotype distribution. PolyPhen-2 and SIFT, two algorithms for predicting the functional effects of amino acid substitutions, showed significant scores for p.R654H. Therefore, R654H might be a risk factor for epidermal barrier dysfunction in some Japanese AD patients.

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  • Type D (CD8+) lymphomatoid papulosis in a patient with classic (CD4+) mycosis fungoides. International journal

    Junko Hori-Kosogabe, Toshihisa Hamada, Shin Morizane, Yoji Hirai, Tomoko Miyake, Tadashi Yoshino, Keiji Iwatsuki

    European journal of dermatology : EJD   28 ( 2 )   267 - 268   2018.4

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  • Epstein-Barr virus-positive mucocutaneous ulcer in a patient with polycythemia vera treated with oral hydroxyurea. International journal

    Toshihisa Hamada, Mariko Kawata, Yoshinobu Maeda, Tadashi Yoshino, Tomoko Miyake, Shin Morizane, Yoji Hirai, Keiji Iwatsuki

    The Journal of dermatology   45 ( 4 )   e82-e83   2018.4

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  • A Case of Psoriasis Complicated by Breast Cancer after Systemic Treatments Including Biologics.

    Shin Morizane, Saeko Sugimoto, Takayuki Motoki, Norihisa Katayama, Masako Omori, Keiji Iwatsuki

    Acta medica Okayama   72 ( 2 )   185 - 187   2018.4

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    Psoriasis is a common chronic inflammatory skin disorder that is characterized by scaly, erythematous, sharply demarcated plaques. The treatment for psoriasis has dramatically changed over the last 10 years with the introduction of biologics. However, the risk of cancer induced by biologics for psoriasis has not been fully analyzed, since these agents have such a short history of use. Here we report the case of a 74-year-old woman with psoriasis vulgaris and psoriatic arthritis complicated by breast cancer after systemic treatments including etretinate, cyclosporine, methotrexate, adalimumab, and ustekinumab.

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  • High calcium enhances the expression of double-stranded RNA sensors and antiviral activity in epidermal keratinocytes. International journal

    Yuriko Yamamura, Shin Morizane, Takenobu Yamamoto, Jun Wada, Keiji Iwatsuki

    Experimental dermatology   27 ( 2 )   129 - 134   2018.2

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    Double-stranded RNA (dsRNA) sensors including TLR3, MDA5 and RIG-I are expressed in epidermal keratinocytes and play an important immunological role by enhancing various innate and adaptive immune responses. Although the role of elevated extracellular calcium concentration in keratinocyte differentiation is well understood, the effect of high calcium on dsRNA sensors is not well studied. We investigated alterations in dsRNA sensor expression and antiviral activity induced by a high extracellular concentration of calcium in epidermal keratinocytes. Normal human epidermal keratinocytes (NHEKs) were stimulated with high calcium and/or synthetic dsRNA, poly (I:C). TLR3, IFIH1 (MDA5) and DDX58 (RIG-I) expression were measured via qPCR, and IFN-β and human beta-defensin 2 (HBD2) levels were measured using ELISA. TLR3 localization was evaluated with immunocytofluorescence. Antiviral activity was quantified with virus plaque assays using herpes simplex virus type 1 (HSV-1). High calcium significantly upregulated mRNA expression of TLR3, IFIH1 and DDX58 in NHEKs. In addition, high calcium significantly enhanced poly (I:C)-induced anti-HSV-1 activity in NHEKs. The antiviral molecule HBD2 but not IFN-β induction by poly (I:C) was enhanced by high calcium. Our findings indicate that high levels of extracellular calcium enhance the expression of dsRNA sensors and augment antiviral activity in epidermal keratinocytes.

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  • Clinical outcomes of primary cutaneous anaplastic large cell lymphoma: data from a single Japanese centre. International journal

    Yuka Maruta, Toshihisa Hamada, Hiroko Fukamatsu, Tatsuya Kaji, Shin Morizane, Yoji Hirai, Tomoko Miyake, Osamu Yamasaki, Tadashi Yoshino, Keiji Iwatsuki

    European journal of dermatology : EJD   27 ( 6 )   669 - 671   2017.12

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  • Two arginine residues in the COOH-terminal of human β-defensin-3 constitute an essential motif for antimicrobial activity and IL-6 production. Reviewed International journal

    Yoko Sakagami-Yasui, Yoshinori Shirafuji, Osamu Yamasaki, Shin Morizane, Toshihisa Hamada, Hiroshi Umemura, Keiji Iwatsuki

    Experimental dermatology   26 ( 11 )   1026 - 1032   2017.11

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    Human β-defensin-3 (HBD-3) possesses antimicrobial activities and the potential to induce proinflammatory cytokines. HBD-3 contains a unique motif of two arginine residues (Arg or R) in the COOH-terminal region. To understand the bioactive properties of the Arg residues of HBD-3, we examined antimicrobial activities against Staphylococcus aureus and Pseudomonas aeruginosa using synthetic HBD-2, HBD-3 and two variant peptides of HBD-3: the Arg-truncated variant designated desR HBD-3 and NRR HBD-3, in which both Arg residues were shifted to the N-terminal region. IL-6 production from keratinocytes was studied using the peptides. HBD-3 possessed approximately five-fold more potent antimicrobial activities, evaluated as the minimum inhibitory concentration (MIC), against S. aureus compared with desR and NRR HBD-3, while no significant activity was observed in HBD-2. The antimicrobial activity of HBD-3 against S. aureus was well preserved even at high sodium chloride concentrations, but was attenuated in desR and NRR HBD-3. All the peptides exhibited similar antimicrobial activities against P. aeruginosa, but HBD-2 and desR HBD-3 showed diminished antimicrobial activities against P. aeruginosa at high salt concentrations. IL-6 production was significantly induced in keratinocytes with HBD-3, but not remarkably with stimulation by other peptide. These Arg residues are essential for the antimicrobial and biological properties of HBD-3.

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  • Possible macrophage activation in melanoma patients receiving combined kinase inhibitor therapy following anti-PD-1 therapy: a cytokine profiling study of two cases. International journal

    H Umemura, O Yamasaki, S Morizane, K Iwatsuki

    Annals of oncology : official journal of the European Society for Medical Oncology   28 ( 10 )   2624 - 2625   2017.10

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  • The Involvement of Serum Amyloid A in Psoriatic Inflammation. International journal

    Shin Morizane, Kazuko Mizuno, Tetsuya Takiguchi, Saeko Sugimoto, Keiji Iwatsuki

    The Journal of investigative dermatology   137 ( 3 )   757 - 760   2017.3

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  • Persistent elevation of serum interleukin-6 and serum amyloid A levels in patients with recessive dystrophic epidermolysis bullosa. International journal

    Shin Morizane, Kazuko Mizuno, Tetsuya Takiguchi, Satoko Ogita, Yoshinobu Nishida, Yoshio Kawakami, Keiji Iwatsuki

    European journal of dermatology : EJD   27 ( 1 )   80 - 81   2017.2

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  • Comparative study on driver mutations in primary and metastatic melanomas at a single Japanese institute: A clue for intra- and inter-tumor heterogeneity. International journal

    Tatsuya Kaji, Osamu Yamasaki, Minoru Takata, Masaki Otsuka, Toshihisa Hamada, Shin Morizane, Kenji Asagoe, Hiroyuki Yanai, Yoji Hirai, Hiroshi Umemura, Keiji Iwatsuki

    Journal of dermatological science   85 ( 1 )   51 - 57   2017.1

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    BACKGROUND: Searching for driver mutations in melanoma is critical to understanding melanoma genesis, progression and response to therapy. OBJECTIVES: We aimed to investigate the frequency and pattern of driver mutations in Japanese primary and metastatic melanomas including cases of unknown primary origin, in relation to their clinicopathologic manifestations. METHODS: Seventy-seven samples from 60 patients with melanoma were screened for 70 driver mutations of 20 oncogenes by Sequenom MelaCarta MassARRAY, and the results for primary and metastatic melanomas were compared. RESULTS: Of 77 tissue samples, BRAF V600E was detected in 21 samples (27%), CDK4 R24C in 7, EPHB6 G404S in 6, BRAF V600K in 2, NEK10 E379K in 2, and CDK4 R24H, NRAS Q61K, NRAS Q61R, KRAS G12A, KIT L576P, KIT V559A, ERBB4 E452K, and PDGFRA E996K in one sample each. No driver mutations related to the MAPK cascade including RAS and BRAF were detected in the chronically sun-damaged (CSD) group of melanoma. Dual or triple driver mutations were found in four of 40 (10%) samples from the primary melanomas, and three of 37 (8%) of the metastatic melanomas. Fourteen of 26 (54%) samples of non-CSD melanoma, and 3 of 6 (50%) melanomas of unknown primary origin had the BRAF V600E mutation. Mutations in membrane-bound receptors including KIT, ERBB4 and EPHB6 were detected in 8 of 77 (10%) samples. Of 17 pairs of primary and metastatic melanomas from the same patient, the primary mutation pattern was changed to a novel one in three cases, and only one of the plural mutations in the primary melanoma was found in the metastatic lesions in two cases. CONCLUSIONS: BRAF V600E is a predominant mutation in non-CSD melanoma and melanomas of unknown primary origin. Mutational heterogeneity may exist in the primary melanoma (intra-tumor heterogeneity), and between the primary and metastatic lesions (inter-tumor heterogeneity).

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  • Identification of an S100A8 Receptor Neuroplastin-β and its Heterodimer Formation with EMMPRIN. International journal

    Masakiyo Sakaguchi, Mami Yamamoto, Masashi Miyai, Tatsuo Maeda, Junichiro Hiruma, Hitoshi Murata, Rie Kinoshita, I Made Winarsa Ruma, Endy Widya Putranto, Yusuke Inoue, Shin Morizane, Nam-Ho Huh, Ryoji Tsuboi, Toshihiko Hibino

    The Journal of investigative dermatology   136 ( 11 )   2240 - 2250   2016.11

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    We previously reported a positive feedback loop between S100A8/A9 and proinflammatory cytokines mediated by extracellular matrix metalloproteinase inducer, an S100A9 receptor. Here, we identify neuroplastin-β as an unreported S100A8 receptor. Neuroplastin-β and extracellular matrix metalloproteinase inducer form homodimers and a heterodimer, and they are co-localized on the surface of cultured normal human keratinocytes. Knockdown of both receptors suppressed cell proliferation and proinflammatory cytokine induction. Upon stimulation with S100A8, neuroplastin-β recruited GRB2 and activated extracellular signal-regulated kinase, resulting in keratinocyte proliferation. Keratinocyte proliferation in response to inflammatory stimuli was accelerated in involucrin promoter-driven S100A8 transgenic mice. Further, S100A8 and S100A9 were strongly up-regulated and co-localized in lesional skin of atopic dermatitis patients. Our results indicate that neuroplastin-β and extracellular matrix metalloproteinase inducer form a functional heterodimeric receptor for S100A8/A9 heterodimer, followed by recruitment of specific adaptor molecules GRB2 and TRAF2, and this signaling pathway is involved in activation of both keratinocyte proliferation and skin inflammation in atopic skin. Suppression of this pathway might have potential for treatment of skin diseases associated with chronic inflammation such as atopic dermatitis.

    DOI: 10.1016/j.jid.2016.06.617

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  • Epstein-Barr virus reactivation is induced, but abortive, in cutaneous lesions of systemic hydroa vacciniforme and hypersensitivity to mosquito bites. International journal

    Takenobu Yamamoto, Yoji Hirai, Tomoko Miyake, Toshihisa Hamada, Osamu Yamasaki, Shin Morizane, Wataru Fujimoto, Keiji Iwatsuki

    Journal of dermatological science   82 ( 3 )   153 - 9   2016.6

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    BACKGROUND: Epstein-Barr virus (EBV)-associated T/natural killer (NK)-lymphoproliferative disorders (LPDs) include hydroa vacciniforme (HV) and hypersensitivity to mosquito bites (HMB). The pathomechanisms of these diseases are still unclear. OBJECTIVE: To understand the inflammatory process, we examined EBV reactivation markers, BZLF1 and BDRF1 mRNA in the tissue and blood from patients with EBV-associated T/NK-LPDs. METHODS: Sixty-four patients with EBV-associated LPDs and epithelial neoplasms, and EBV+ cell line cells were studied. DNase-treated and resistant EBV DNA load in blood and cell culture supernatants were calculated. An EBV reactivation signal was analyzed in the tissue, blood and cell line cells. RESULTS: In the tissue, BZLF1 mRNA was detected in 5 of 6 (83%) samples of EBV+ epithelial neoplasms, 16 of 21 (76%) of EBV+ lymphomas, and 5 of 15 (33%) of systemic HV and/or HMB, but negative in all 15 patients with classical HV. In the blood, BZLF1 mRNA was detected in only one of 19 (5.3%) samples of EBV-associated T/NK-LPDs. A down-stream reactivation signal, BDRF1 mRNA was expressed in all 6 samples of EBV+ epithelial neoplasms, but it was positive in only one of 15 (6.7%) samples from systemic HV and HMB in the tissue. EBV+ T/NK-cell line cells treated with phorbol 12-myristate 13-acetate produced BZLF1 and BDRF1 mRNA, and encapsidated EBV DNA was detected in the culture supernatants of cell line cells. CONCLUSION: Stimulation-induced EBV reactivation occurred both in vivo and in vitro, but it was almost abortive in vivo. Reactivation-related EBV antigens might be responsible for induction of systemic HV and HMB.

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  • Dexamethasone but not tacrolimus suppresses TNF-α-induced thymic stromal lymphopoietin expression in lesional keratinocytes of atopic dermatitis model. International journal

    Kazuko Mizuno, Shin Morizane, Tetsuya Takiguchi, Keiji Iwatsuki

    Journal of dermatological science   80 ( 1 )   45 - 53   2015.10

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    BACKGROUND: Thymic stromal lymphopoietin (TSLP) initiates the Th2-type allergic inflammation, and is thought to play an important role in the pathogenesis of atopic dermatitis (AD). TNF-α is a key cytokine which is involved in the pathophysiology of various inflammatory diseases, and the expression level is elevated in the sera and skin of patients with AD. In addition, TNF-α has been reported to induce TSLP expression in epidermal keratinocytes. Topical glucocorticoids and calcineurin inhibitors are safe and effective agents for AD, but the effects of these agents on TNF-α-induced TSLP expression are not fully understood. OBJECTIVE: To investigate whether the glucocorticosteroid dexamethasone and the calcineurin inhibitor tacrolimus could affect TSLP expression induced by TNF-α in lesional keratinocytes of AD. METHODS: The effects of topical dexamethasone and tacrolimus on TSLP expression were evaluated in an AD mouse model induced by repeated 2,4,6-trinitro-1-chlorobenzene application. Co-immunostaining for TSLP and TNF-α was performed using skin samples from AD patients and the mouse model. Normal human epidermal keratinocytes (NHEKs) were cultured with dexamethasone or tacrolimus in the presence of TNF-α to analyze TSLP expression. RESULTS: Topical application of dexamethasone but not tacrolimus repressed TSLP expression in the mouse model. TSLP and TNF-α showed similar distribution pattern in epidermal keratinocytes of AD lesions and the mouse model. TSLP expression was induced by TNF-α via NF-κB in a dose-dependent and an autocrine and/or paracrine manner in NHEKs, which was significantly suppressed by dexamethasone but not by tacrolimus. Similarly to TSLP expression, IL-6, TNF-α, IL-8, and IL-36γ expression induced by TNF-α were significantly suppressed by dexamethasone but not by tacrolimus in NHEKs. CONCLUSION: Dexamethasone but not tacrolimus suppresses the TSLP expression induced by TNF-α in lesional keratinocytes of AD model. Our observations uncover the unreported functional difference between topical glucocorticosteroids and calcineurin inhibitors in cutaneous inflammatory diseases.

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  • Interferon-Gamma Enhances TLR3 Expression and Anti-Viral Activity in Keratinocytes. Reviewed International journal

    A I Kajita, Shin Morizane, Tetsuya Takiguchi, Takenobu Yamamoto, Masao Yamada, Keiji Iwatsuki

    The Journal of investigative dermatology   135 ( 8 )   2005 - 2011   2015.8

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    Toll-like receptors (TLRs) recognize specific microbial products in the innate immune response. TLR3, a double-stranded RNA sensor, is thought to have an important role in viral infections, but the regulation of TLR3 expression and its function in keratinocytes are not fully understood. Here we show the Th1 cytokine IFN-γ increased the TLR3 expression via STAT1 in cultured normal human epidermal keratinocytes (NHEKs). Co-stimulation with IFN-γ and the TLR3 ligand poly (I:C) synergistically increased the expression of IFN-β, IL-6, IL-8, and human β-defensin-2 in NHEKs compared with poly (I:C) or IFN-γ alone. These synergistic inductions were significantly inhibited by an endosomal acidification inhibitor, chloroquine, and by TLR3 siRNA. Co-stimulation with IFN-γ and poly (I:C) also significantly enhanced the anti-viral activity against herpes simplex virus type-1 in NHEKs compared with poly (I:C) or IFN-γ alone. In addition to the in vitro findings, an immunohistochemical analysis revealed IFN-γ-positive cells surrounding herpetic vesicles. These findings indicate that IFN-γ might contribute to the innate immune response to cutaneous viral infections by enhancing TLR3 expression and function in keratinocytes.

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  • Survival rates and prognostic factors of Epstein-Barr virus-associated hydroa vacciniforme and hypersensitivity to mosquito bites

    T. Miyake, T. Yamamoto, Y. Hirai, M. Otsuka, T. Hamada, K. Tsuji, S. Morizane, D. Suzuki, Y. Aoyama, K. Iwatsuki

    British Journal of Dermatology   172 ( 1 )   56 - 63   2014.12

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  • Immune escape phenomenon in molluscum contagiosum and the induction of apoptosis. International journal

    Akiko Yamauchi-Yamada, Takenobu Yamamoto, Yumi Nakayama, Kazuko Ikeda, Tomoko Miyake, Mari Yamaguchi, Yoji Hirai, Yoshinori Shirafuji, Shin Morizane, Yumi Aoyama, Keiji Iwatsuki

    The Journal of dermatology   41 ( 12 )   1058 - 64   2014.12

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    Molluscum contagiosum (MC) may persist for many weeks, evading host immunity. We studied the mechanism of immune escape phenomenon in MC, and the possible inducer of apoptosis. Using tissue samples of MC, we examined the numbers of epidermal Langerhans cells (LC), the expression levels of macrophage inflammatory protein-3α (MIP-3α) and thymic stromal lymphopoietin (TSLP), and the apoptotic signals. After molluscum contagiosum virus (MCV) genotyping, we studied the expression of MCV-encoded MC148 mRNA and MC159 mRNA which correspond to viral antagonist for CCR8 and viral Fas-linked interleukin (IL)-1β converting enzyme (FLICE)-like inhibitor protein (vFLIP), respectively. The nutlin-3-induced apoptosis in MC was observed ex vivo. The numbers of CD1a(+) or Langerin(+) epidermal LC and the expression levels of MIP-3α were markedly decreased in MC. The expression of TSLP was enhanced in the lesional epidermis of atopic dermatitis and human papillomavirus-induced warts, whereas the expression was observed locally in MC. All 14 MC samples examined harbored MCV type 1. The MC148 mRNA was detected in all 14 samples and the MC159 mRNA was detected in 13 samples. Apoptotic cells were absent or at a background level in the living layers of MC, but their numbers were increased in the molluscum bodies by overnight incubation with 5 μmol/L nutlin-3 in culture medium. In conclusion, molluscum bodies are protected from host immune responses and apoptotic signals by being surrounded by LC-depleted epidermal walls and viral immunosuppressive molecules, but could be eradicated by reagents inducing p53-dependent apoptosis.

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  • Cathelicidin antimicrobial peptide LL-37 augments interferon-beta expression and antiviral activity induced by double-stranded RNA in keratinocytes

    Takiguchi T, Morizane S, Yamamoto T, Kajita A, Ikeda K, Iwatsuki K

    British Journal of Dermatology   171 ( 3 )   492 - 498   2014.9

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  • Folliculotropic mycosis fungoides with extreme pilosebaceous involvement and hematological abnormalities

    Kazuko Mizuno-Ikeda, Toshihisa Hamada, Daisuke Suzuki, Shin Morizane, Toshiyuki Watanabe, Masahide Imada, Ken Okada, Keiji Iwatsuki

    European Journal of Dermatology   24 ( 1 )   135 - 136   2014.1

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  • A higher correlation of the antibody activities against the calcium-dependent epitopes of desmoglein 3 quantified by ethylenediaminetetraacetic acid-treated enzyme-linked immunosorbent assay with clinical disease activities of pemphigus vulgaris. International journal

    Koji Kamiya, Yumi Aoyama, Yoshinori Shirafuji, Toshihisa Hamada, Shin Morizane, Kazuyasu Fujii, Keiji Iwatsuki

    Journal of dermatological science   70 ( 3 )   190 - 5   2013.6

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    BACKGROUND: Nonpathogenic anti-desmoglein (Dsg) 3 antibodies can be found in pemphigus vulgaris (PV) patients' sera. Previously, ethylenediaminetetraacetic acid (EDTA)-treated enzyme-linked immunosorbent assay (ELISA) was found to detect only nonpathogenic anti-Dsg3 antibodies against the non-calcium (Ca(2+))-dependent epitopes. OBJECTIVE: We examined whether the calculated anti-Dsg3 antibody titer for Ca(2+)-dependent epitopes, using the conventional and EDTA-treated ELISAs, correlated better with the disease activity of PV. METHODS: We analyzed 123 serum samples from 19 PV patients. Of these samples, there were 52 samples from 15 PV patients obtained in asymptomatic phases. The difference between conventional Dsg3 ELISA index and EDTA-treated Dsg3 ELISA index was calculated as the anti-Dsg3 antibody activity for Ca(2+)-dependent conformational epitopes (conformational Dsg3 ELISA index). We analyzed the correlation between Dsg3 ELISA index values and the pemphigus disease area index (PDAI). Moreover, we examined whether the conformational Dsg3 ELISA index fluctuated in parallel with the disease activity during clinical courses of 6 PV patients. We evaluated the pathogenicity of anti-Dsg3 antibodies detected in remission phases using a dissociation assay. RESULTS: The conventional Dsg3 ELISA index showed a high positive rate in the asymptomatic phase of PV. By contrast, the conformational Dsg3 ELISA index showed a much closer correlation to the disease activity when monitored in individual cases. Nonpathogenic anti-Dsg3 antibodies were detected in these cases. CONCLUSION: The conformational Dsg3 ELISA index reflected the pathogenicity of anti-Dsg3 antibodies more accurately than the conventional Dsg3 ELISA index. Using both conventional and EDTA-treated ELISAs would be useful in monitoring the disease activity of PV.

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  • Immunological and structural remodeling in human papillomavirus-induced warts and Bowen disease. International journal

    Keiji Iwatsuki, Yumi Nakayama, Toshihisa Hamada, Gen Nakanishi, Yoshinori Shirafuji, Shin Morizane

    European journal of dermatology : EJD   2013.4

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    Human papillomavirus-associated warts (HPV-warts) are persistent, evading host immune surveillance. However, these warts sometimes disappear spontaneously, following inflammation. Non-inflamed HPV-warts demonstrated decreased numbers of epidermal Langerhans cells (LCs), low expression levels of MIP3α and E-cadherin, and no apoptotic cells. In the inflamed HPV-warts, on the other hand, various dendritic cell (DC) subsets and many CD8+ cytotoxic T lymphocytes (CTLs) were recruited in association with epidermal MIP3α expression. Many apoptotic keratinocytes were observed in the dermo-epidermal junction. Cellular events were different in HPV-induced Bowen disease (HPV-Bowen): a few LCs were retained in the lesional epidermis, and considerable numbers of B-cells and plasma cells were also observed in the infiltrates, with little or no infiltration of plasmacytoid DCs or dermal/mature DCs. Multiple HPV16-Bowen diseases in the same individuals showed the presence of different sizes of E6/E7-containing cellular transcripts, which indicated that HPV genomes were integrated into the different sites of chromosomes. Toll-like receptor (TLR) 3 was expressed by the lesional keratinocytes even in the non-inflamed HPV-warts, and type 1 interferons (IFNs) were produced in cultured keratinocytes by TLR3 stimulation. HPV-warts are protected from host immune responses and apoptotic signals because they are surrounded by LC-depleted epidermal walls, and viral anti-apoptotic molecules. The up-regulation of epidermal TLR3 signaling might inhibit further HPV spreading.

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  • Eccrine poromatosis associated with polychemotherapy. International journal

    Kazuyasu Fujii, Seiko Aochi, Chinatsu Takeshima, Masaki Ohtsuka, Toshihisa Hamada, Kenji Asagoe, Yumi Aoyama, Shin Morizane, Keiji Iwatsuki

    Acta dermato-venereologica   92 ( 6 )   687 - 90   2012.11

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    Eccrine poroma frequently occurs as a solitary tumour, and only a few reports have described the occurrence of multiple lesions. Multiple eccrine poromas, or eccrine poromatosis, may occur in patients who have undergone radiotherapy and/or polychemotherapy. We report here four cases of multiple eccrine poromas in patients who were either undergoing, or had undergone, intensive chemotherapy (from 6 months to 16 years prior to onset). Three patients had non-Hodgkin's lymphoma and one had malignant fibrous histiocytosis. The number of lesions varied from 3 to > 20 in each patient, and all the lesions occurred on non-irradiated skin. The histopathological features were consistent with those of eccrine poroma, Pinkus type. In addition to radiation therapy, intensive chemotherapy may play a role in the pathogenesis of multiple eccrine poromas even many years after treatment.

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  • The antimicrobial protein REG3A regulates keratinocyte proliferation and differentiation after skin injury. International journal

    Yuping Lai, Dongqing Li, Changwei Li, Beda Muehleisen, Katherine A Radek, Hyun Jeong Park, Ziwei Jiang, Zhiheng Li, Hu Lei, Yanchun Quan, Tian Zhang, Yelin Wu, Paul Kotol, Shin Morizane, Tissa R Hata, Keiji Iwatsuki, Ce Tang, Richard L Gallo

    Immunity   37 ( 1 )   74 - 84   2012.7

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    Epithelial keratinocyte proliferation is an essential element of wound repair, and abnormal epithelial proliferation is an intrinsic element in the skin disorder psoriasis. The factors that trigger epithelial proliferation in these inflammatory processes are incompletely understood. Here we have shown that regenerating islet-derived protein 3-alpha (REG3A) is highly expressed in keratinocytes during psoriasis and wound repair and in imiquimod-induced psoriatic skin lesions. The expression of REG3A by keratinocytes is induced by interleukin-17 (IL-17) via activation of keratinocyte-encoded IL-17 receptor A (IL-17RA) and feeds back on keratinocytes to inhibit terminal differentiation and increase cell proliferation by binding to exostosin-like 3 (EXTL3) followed by activation of phosphatidylinositol 3 kinase (PI3K) and the kinase AKT. These findings reveal that REG3A, a secreted intestinal antimicrobial protein, can promote skin keratinocyte proliferation and can be induced by IL-17. This observation suggests that REG3A may mediate the epidermal hyperproliferation observed in normal wound repair and in psoriasis.

    DOI: 10.1016/j.immuni.2012.04.010

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  • TH2 cytokines increase kallikrein 7 expression and function in patients with atopic dermatitis. International journal

    Shin Morizane, Kenshi Yamasaki, Ai Kajita, Kazuko Ikeda, Maosheng Zhan, Yumi Aoyama, Richard L Gallo, Keiji Iwatsuki

    The Journal of allergy and clinical immunology   130 ( 1 )   259 - 61   2012.7

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  • Hydroa vacciniforme is associated with increased numbers of Epstein-Barr virus-infected γδT cells. International journal

    Yoji Hirai, Takenobu Yamamoto, Hiroshi Kimura, Yoshinori Ito, Kazuhide Tsuji, Tomoko Miyake, Shin Morizane, Daisuke Suzuki, Kazuyasu Fujii, Keiji Iwatsuki

    The Journal of investigative dermatology   132 ( 5 )   1401 - 8   2012.5

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    Hydroa vacciniforme (HV) is a rare photosensitivity disorder of childhood associated with Epstein-Barr virus (EBV)(+) T-cell infiltration. We have summarized clinical manifestations of HV, and analyzed EBV(+) T-cell subsets as well as EBV DNA load in lymphocyte fractions, in comparison with hypersensitivity to mosquito bites (HMB), an EBV-associated T/natural killer (NK) lymphoproliferative disorder. We found that 31 of 33 (93.9%) HV lesions were composed of EBV(+) T cells and reactive EBV(-) cytotoxic T cells, without significant CD56(+) cell infiltration, whereas many CD56(+) cells were present in 8 of 9 (88.9%) HMB lesions. Of 13 (20.6%) HMB patients with or without HV, 12 (92.3%) showed increased percentages (>32%) of NK cells in the peripheral blood, whereas in the 16 patients with HV alone, 14 (87.5%) showed no increase. Of the 11 HV patients, 10 (90.9%) had increased percentages (>5%) of circulating γδT cells, with a mean value of 15.7 ± 2.9%, and the γδT-cell fractions contained larger amounts of EBV DNA than non-γδT-cell fractions. A triple-labeling method revealed that all three HV patients examined had increased percentages of EBER(+), T-cell receptor (TCR)γδ(+), and TCRαβ(-) cells. Our observations indicate that HV is associated with increased numbers of EBV(+) γδT cells, whereas HMB is associated with EBV(+) NK cells.

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  • Antimicrobial peptides in the pathogenesis of psoriasis. International journal

    Shin Morizane, Richard L Gallo

    The Journal of dermatology   39 ( 3 )   225 - 30   2012.3

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    One characteristic abnormality of lesional skin in psoriasis is the excessive production of antimicrobial peptides and proteins (AMPs). AMPs typically are small (12-50 amino acids), have positive charge and amphipathic structure, and are found in all living organisms including mammals, insects, plants and invertebrates. These peptides are best known for their integral role in killing pathogenic microorganisms; however, in vertebrates, they are also capable of modifying host inflammatory responses by a variety of mechanisms. In psoriatic lesions, many AMPs are highly expressed, and especially the associations between psoriasis and cathelicidin, β-defensins or S100 proteins have been well studied. Among them, a cathelicidin peptide, LL-37, has been highlighted as a modulator of psoriasis development in recent years. AMPs had been thought to worsen psoriatic lesions but recent evidence has also suggested the possibility that the induction of AMPs expression might improve aspects of the disease. Further investigations are needed to uncover a previously underappreciated role for AMPs in modulating the immune response in psoriasis, and to improve disease without the risks of systemic immunosuppressive approaches.

    DOI: 10.1111/j.1346-8138.2011.01483.x

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  • Cathelicidin antimicrobial peptide LL-37 in psoriasis enables keratinocyte reactivity against TLR9 ligands. International journal

    Shin Morizane, Kenshi Yamasaki, Beda Mühleisen, Paul F Kotol, Masamoto Murakami, Yumi Aoyama, Keiji Iwatsuki, Tissa Hata, Richard L Gallo

    The Journal of investigative dermatology   132 ( 1 )   135 - 43   2012.1

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    Here we show that keratinocytes in psoriatic lesional skin express increased Toll-like receptor (TLR) 9 that similarly localizes with elevated expression of the cathelicidin antimicrobial peptide LL-37. In culture, normal human keratinocytes exposed to LL-37 increased TLR9 expression. Furthermore, when keratinocytes were exposed to LL-37 and subsequently treated with TLR9 ligands, such as CpG or genomic DNA, they greatly increased production of type I IFNs. This response mimicked observations in the epidermis of psoriatic lesional skin as keratinocytes in psoriatic lesions produce greater amounts of IFN-β than normal skin lacking LL-37. The mechanism for induction of type I IFNs in keratinocytes was dependent on TLR9 expression but not on a DNA-LL-37 complex. These findings suggest that keratinocytes recognize and respond to DNA and can actively participate in contributing to the immunological environment that characterizes psoriasis.

    DOI: 10.1038/jid.2011.259

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  • Dendritic cell subsets and immunological milieu in inflammatory human papilloma virus-related skin lesions. International journal

    Yumi Nakayama, Kenji Asagoe, Akiko Yamauchi, Takenobu Yamamoto, Yoshinori Shirafuji, Shin Morizane, Gen Nakanishi, Keiji Iwatsuki

    Journal of dermatological science   63 ( 3 )   173 - 83   2011.9

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    BACKGROUND: Human papilloma virus (HPV)-related warts persist, evading host immune surveillance, but sometimes disappear with inflammation. OBJECTIVES: To elucidate the immune evasion mechanisms of HPV, we have examined the density, dynamics, and subsets of dendritic cell (DC) types in non-inflammatory or inflammatory HPV-related skin lesions such as warts and Bowen's disease (HPV-Bowen), and compared the epidermal expression levels of macrophage inflammatory protein (MIP)-3α and E-cadherin. METHODS: The expression of various DC markers, MIP-3α, and E-cadherin in the tissue samples obtained from patients with warts, HPV-Bowen and HPV-unrelated skin diseases was evaluated by immunohistochemistry. MIP-3α gene expression levels were examined in warts and HPV-Bowen by in situ hybridization (ISH) and real-time quantitative polymerase chain reaction (RT-qPCR). RESULTS: The numbers of Langerhans cells (LCs) and the expression levels of MIP-3α and E-cadherin were decreased in non-inflammatory warts and HPV-Bowen, as compared with normal skin. Both epidermal LCs and MIP-3α expression reappeared in inflammatory warts, associated with dermal infiltrates composed of many cytotoxic T cells and various subsets of DCs, while cellular infiltrates in HPV-Bowen contained many B cells and plasma cells with sparse infiltration of DCs. The upregulation of MIP-3α gene expression was confirmed in the inflammatory warts and HPV-Bowen by ISH and RT-qPCR. CONCLUSIONS: The depletion of LCs in the non-inflammatory warts and HPV-Bowen is associated with a down-regulation of expression levels of MIP-3α and E-cadherin in the lesional keratinocytes. MIP-3α expression is upregulated in lesional keratinocytes of inflammatory warts, with the subsequent recruitment of various DC subsets and cytotoxic T cells, whereas plasma cell-rich infiltration was induced in HPV-Bowen.

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  • TLR2 expression is increased in rosacea and stimulates enhanced serine protease production by keratinocytes. International journal

    Kenshi Yamasaki, Kimberly Kanada, Daniel T Macleod, Andrew W Borkowski, Shin Morizane, Teruaki Nakatsuji, Anna L Cogen, Richard L Gallo

    The Journal of investigative dermatology   131 ( 3 )   688 - 97   2011.3

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    A diverse environment challenges skin to maintain temperature, hydration, and electrolyte balance while also maintaining normal immunological function. Rosacea is a common skin disease that manifests unique inflammatory responses to normal environmental stimuli. We hypothesized that abnormal function of innate immune pattern recognition could explain the enhanced sensitivity of patients with rosacea, and observed that the epidermis of patients with rosacea expressed higher amounts of Toll-like receptor 2 (TLR2) than normal patients. Increased expression of TLR2 was not seen in other inflammatory skin disorders such as atopic dermatitis or psoriasis. Overexpression of TLR2 on keratinocytes, treatment with TLR2 ligands, and analysis of TLR2-deficient mice resulted in a calcium-dependent release of kallikrein 5 from keratinocytes, a critical protease involved in the pathogenesis of rosacea. These observations show that abnormal TLR2 function may explain enhanced inflammatory responses to environmental stimuli and can act as a critical element in the pathogenesis of rosacea.

    DOI: 10.1038/jid.2010.351

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  • Bilateral conjunctival lesions in blastic plasmacytoid dendritic cell neoplasm.

    Toshihiko Matsuo, Kouichi Ichimura, Takehiro Tanaka, Shin Morizane, Keiji Iwatsuki, Motoharu Eguchi, Tadashi Yoshino

    Journal of clinical and experimental hematopathology : JCEH   51 ( 1 )   49 - 55   2011

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    The purpose of this study is to report on a patient who developed conjunctival lesions of blastic plasmacytoid dendritic cell neoplasm (BPDCN) after radiation to skin lesions of the same pathological type. A 79-year-old man developed salmon-pink lesions in the lower fornix of the conjunctiva of both eyes and biopsy revealed BPDCN. One and a half years previously, he noticed an erythematous plaque with a 30-mm diameter, which later became multiple, on the left chest, and the biopsy revealed BPDCN. The bone marrow was negative for CD56-positive cells, but fluorescence-activated cell sorting analysis of peripheral blood cells revealed a group of cells positive for CD4 and CD56, or CD4 and CD123. The monocyte fraction, in an increased percentage of white blood cell counts, did contain atypical cells positive for the three markers. Whole-body 2-[(18)F]fluoro-2-deoxy-D-glucose positron emission tomography combined with computed tomography demonstrated no abnormal uptake lesions. He received 30 Gy of radiation to the chest lesions, and later, the same dose of radiation to novel skin lesions on the back, waist, and chest, and also to pharyngeal lesions. In conclusion, the conjunctiva could be involved with BPDCN and pathological differential diagnosis by biopsy is mandatory to establish the correct diagnosis.

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  • Kallikrein expression and cathelicidin processing are independently controlled in keratinocytes by calcium, vitamin D(3), and retinoic acid. International journal

    Shin Morizane, Kenshi Yamasaki, Filamer D Kabigting, Richard L Gallo

    The Journal of investigative dermatology   130 ( 5 )   1297 - 306   2010.5

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    Cathelicidin has dual functions in the skin, acting as an innate antibiotic and as an immunomodulator in diseases such as rosacea and psoriasis. The serine proteases kallikrein 5 (KLK5) and kallikrein 7 (KLK7) control enzymatic processing of cathelicidin precursor in the skin and regulate the eventual function of the final forms of these peptides. We analyzed factors that control expression of KLK5 and KLK7 in normal human epidermal keratinocytes to better understand how these may influence cathelicidin processing and function. Increased extracellular calcium-induced KLK5 and KLK7 mRNA expression and protein release in a time-dependent manner that is similar to induction of differentiation markers such as keratin 10 and involucrin. However, 1,25(OH)(2) vitamin D(3), 9-cis retinoic acid (RA), and 13-cis RA also induced the KLKs, but the timing and pattern of KLK induction for each were different and distinct from changes in differentiation markers. Increased protease activity and differential processing of cathelicidin accompanied increased KLK expression. These findings show that the expression and activity of KLK are under fine control and can be distinctly influenced by variables such as differentiation, calcium, vitamin D, and RA. Thus, these variables may further control the functions of antimicrobial peptides in the skin.

    DOI: 10.1038/jid.2009.435

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  • The host defense peptide cathelicidin is required for NK cell-mediated suppression of tumor growth. International journal

    Amanda S Büchau, Shin Morizane, Janet Trowbridge, Jürgen Schauber, Paul Kotol, Jack D Bui, Richard L Gallo

    Journal of immunology (Baltimore, Md. : 1950)   184 ( 1 )   369 - 78   2010.1

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    Tumor surveillance requires the interaction of multiple molecules and cells that participate in innate and the adaptive immunity. Cathelicidin was initially identified as an antimicrobial peptide, although it is now clear that it fulfills a variety of immune functions beyond microbial killing. Recent data have suggested contrasting roles for cathelicidin in tumor development. Because its role in tumor surveillance is not well understood, we investigated the requirement of cathelicidin in controlling transplantable tumors in mice. Cathelicidin was observed to be abundant in tumor-infiltrating NK1.1(+) cells in mice. The importance of this finding was demonstrated by the fact that cathelicidin knockout mice (Camp(-/-)) permitted faster tumor growth than wild type controls in two different xenograft tumor mouse models (B16.F10 and RMA-S). Functional in vitro analyses found that NK cells derived from Camp(-/-) versus wild type mice showed impaired cytotoxic activity toward tumor targets. These findings could not be solely attributed to an observed perforin deficiency in freshly isolated Camp(-/-) NK cells, because this deficiency could be partially restored by IL-2 treatment, whereas cytotoxic activity was still defective in IL-2-activated Camp(-/-) NK cells. Thus, we demonstrate a previously unrecognized role of cathelicidin in NK cell antitumor function.

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  • Bcl-3 acts as an innate immune modulator by controlling antimicrobial responses in keratinocytes. International journal

    Amanda S Büchau, Daniel T MacLeod, Shin Morizane, Paul F Kotol, Tissa Hata, Richard L Gallo

    The Journal of investigative dermatology   129 ( 9 )   2148 - 55   2009.9

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    Innate immune responses involve the production of antimicrobial peptides (AMPs), chemokines, and cytokines. We report here the identification of B-cell leukemia (Bcl)-3 as a modulator of innate immune signaling in keratinocytes. In this study, it is shown that Bcl-3 is inducible by the Th2 cytokines IL-4 and IL-13 and is overexpressed in lesional skin of atopic dermatitis (AD) patients. Bcl-3 was shown to be important to cutaneous innate immune responses as silencing of Bcl-3 by small-interfering RNA (siRNA) reversed the downregulatory effect of IL-4 on the HBD3 expression. Bcl-3 silencing enhanced vitamin D3 (1,25D3)-induced gene expression of cathelicidin AMP in keratinocytes, suggesting a negative regulatory function on cathelicidin transcription. Furthermore, 1,25D3 suppressed Bcl-3 expression in vitro and in vivo. This study identified Bcl-3 as an important modulator of cutaneous innate immune responses and its possible therapeutic role in AD.

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  • Ichthyosiform eruptions in association with primary cutaneous T-cell lymphomas. International journal

    S Morizane, N Setsu, T Yamamoto, T Hamada, G Nakanishi, K Asagoe, K Iwatsuki

    The British journal of dermatology   161 ( 1 )   115 - 20   2009.7

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    BACKGROUND: Malignant lymphoma is occasionally complicated by ichthyosiform eruptions. OBJECTIVES: To analyse histopathologically the ichthyosiform eruptions associated with cutaneous lymphomas. METHODS: We reviewed the files of patients with malignant lymphoma seen in our dermatology department between January 2001 and May 2006 to search for patients with ichthyosiform eruptions. RESULTS: In our series, nine of 106 patients with malignant lymphomas had ichthyosiform eruptions during their clinical courses, including three (30%) of 10 patients with anaplastic large cell lymphoma (ALCL) and six (14%) of 44 patients with mycosis fungoides (MF). None of the 18 patients with cutaneous B-cell lymphoma had ichthyosiform eruptions. The three patients with ALCL had ichthyosiform eruptions histopathologically consistent with acquired ichthyosis (AI) in which packed horny layers and thin granular layers were present without lymphocytic infiltration. In contrast, four of the six patients with MF (stages Ib and IIb) had ichthyosiform eruptions with epidermotropic infiltration of atypical lymphocytes, as observed in ichthyosiform MF (IMF). Of the remaining two patients, one showed histopathological features overlapping AI and IMF, and the other had AI alone. These two patients (stages IVa and IIb) had tumours composed of CD30+ cells. Filaggrin expression was markedly diminished in both AI and IMF-like eruptions, similar to that of inherited ichthyosis vulgaris. CONCLUSIONS: Ichthyosiform eruptions are often associated with ALCL and MF and can be classified into three groups: AI associated with ALCL and MF expressing CD30, IMF, and their overlap.

    DOI: 10.1111/j.1365-2133.2009.09109.x

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  • IL-17A enhances vitamin D3-induced expression of cathelicidin antimicrobial peptide in human keratinocytes. International journal

    Mark Peric, Sarah Koglin, Song-Min Kim, Shin Morizane, Robert Besch, Jörg C Prinz, Thomas Ruzicka, Richard L Gallo, Jürgen Schauber

    Journal of immunology (Baltimore, Md. : 1950)   181 ( 12 )   8504 - 12   2008.12

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    Cathelicidin is strongly expressed in lesional skin in psoriasis and may play an important role as both an antimicrobial peptide and as an autoinflammatory mediator in this chronic skin disease. The mechanism of increased cathelicidin in psoriatic keratinocytes is not known, but recent observations have found that psoriasis has abundant Th17 cells that produce IL-17A and IL-22. We found that human keratinocytes stimulated with supernatants from T cells isolated from lesional psoriatic skin increased expression of cathelicidin when stimulated in the presence of 1,25-dihydroxyvitamin D(3) (1,25D(3)). This increase was signaled through the IL-17RA. In vitro, IL-17A, but not IL-22, enhanced cathelicidin mRNA and peptide expression in keratinocytes dependent on the presence of 1,25D(3). At the same time, coincubation with 1,25D(3) blocked induction of human beta-defensin 2 (HBD2), IL-6, and IL-8, which are other target genes of IL-17A. Act1, an adaptor associated with IL-17RA and essential for IL-17A signaling, mediated cathelicidin induction, as its suppression by small interfering RNA inhibited HBD2 and cathelicidin. Both, 1,25D(3) and IL-17A signaled cathelicidin induction through MEK-ERK. These results suggest that increased IL-17A in psoriatic skin increases cathelicidin through a vitamin D(3)-, Act1-, and MEK-ERK-dependent mechanism. Therapy targeting this cathelicidin-regulating system might be beneficial in patients suffering from psoriasis.

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  • Karyotypic analysis of bone marrow cells in pyodermic lesions associated with myelodysplastic syndrome. International journal

    Toshihisa Hamada, Hironori Matsuura, Takashi Oono, Shin Morizane, Osamu Yamasaki, Kenji Asagoe, Takenobu Yamamoto, Kazuhide Tsuji, Keiji Iwatsuki

    Archives of dermatology   144 ( 5 )   643 - 8   2008.5

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    BACKGROUND: Recalcitrant pyodermic lesions and neutrophilic dermatoses are often associated with subclinical myelodysplastic syndrome (MDS). In this case series, we assessed the diagnostic importance of karyotypic analysis of bone marrow cells in 4 patients with MDS-associated pyodermic eruptions treated at our university hospital. Karyotypic analysis was performed in bone marrow cells and peripheral blood lymphocytes obtained. Serum levels of granulocyte colony-stimulating factor were measured. OBSERVATIONS: Four patients with pyodermic eruptions or neutrophilic dermatosis had chromosomal abnormalities in bone marrow cells, including del(20)(q11;q13.3) in 2 patients, trisomy 8 in 1 patient, and t(11;22)(q23;q11) in 1 patient. Three patients without morphologic findings suggestive of MDS were diagnosed as having refractory anemia. One female patient had refractory anemia with ringed sideroblasts associated with del(20). Two patients with refractory anemia had a normal karyotype in peripheral blood lymphocytes. Two patients with elevated serum levels of granulocyte colony-stimulating factor had more active or widespread cutaneous diseases. CONCLUSIONS: Karyotypic analysis of bone marrow cells, but not of peripheral blood lymphocytes, is essential in proving a diagnosis of MDS-associated pyodermic lesions. The overexpression of granulocyte colony-stimulating factor, which may compensate for impaired hematopoiesis in patients with MDS, seems to be a key cytokine leading to neutrophilic infiltration.

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  • A novel, noninvasive diagnostic probe for hydroa vacciniforme and related disorders: detection of latency-associated Epstein-Barr virus transcripts in the crusts. International journal

    Takenobu Yamamoto, Kazuhide Tsuji, Daisuke Suzuki, Shin Morizane, Keiji Iwatsuki

    Journal of microbiological methods   68 ( 2 )   403 - 7   2007.2

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    OBJECTIVE: To establish a new diagnostic method for Epstein-Barr virus (EBV)-associated cutaneous disorders. DESIGN: Skin biopsy is usually required to confirm the latent EBV infections in cutaneous lesions of EBV-associated NK/T-cell lymphoproliferative disorders, including hydroa vacciniforme (HV) and hypersensitivity to mosquito bites (HMB). We have devised a novel, noninvasive method to detect EBV-encoded small RNA (EBER), BamHI A rightward transcripts (BARTs) in the skin crusts and scales of such patients. PATIENTS: Six patients with EBV-associated cutaneous lesions were enrolled in the present study, including three patients with HV, one with HV-like eruptions and chronic active EBV infection, and two with EBV-associated cutaneous lymphoma. MAIN OUTCOME MEASURES: RNA was extracted from the crusts obtained from the cutaneous lesions by forceps, converted to cDNA, and processed for polymerase chain reaction (PCR) amplification with a specific set of primers. The PCR products were assayed by a DNA sequencer. RESULTS: Intact RNAs were successfully extracted from the crusts as well as control materials. EBER1 and BARTs RNAs were detected in all 7 crusts, and in 6 of 7 crusts of EBV-associated cutaneous diseases, respectively. One of 23 crusts from non EBV-associated diseases was positive for EBER1 RNA. The sensitivity and specificity of our assay for latent EBV infection were 100% and 95.8% for EBER1 RNA, and 85.7% and 100% for BARTs mRNA, respectively. The correct DNA sequence for EBER1 and BARTs was confirmed in the PCR products by a direct sequencing method. CONCLUSIONS: Our procedure may be of use as a biomarker for EBV-associated cutaneous lesions, including HV, HMB, and NK/T-cell lymphomas.

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  • A novel, noninvasive diagnostic probe for hydroa vacciniforme and related disorders

    Takenobu Yamamoto, Kazuhide Tsuji, Daisuke Suzuki, Shin Morizane, Keiji Iwatsuki

    Journal of Microbiological Methods   68 ( 2 )   403 - 407   2007.2

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  • Staphylococcal cutaneous infections: invasion, evasion and aggression. International journal

    Keiji Iwatsuki, Osamu Yamasaki, Shin Morizane, Takashi Oono

    Journal of dermatological science   42 ( 3 )   203 - 14   2006.6

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    Staphylococcal infections cause a variety of cutaneous and systemic infections, including impetigo, furuncle, subcutaneous abscess, staphylococcal scalded skin syndrome (SSSS), toxic shock syndrome (TSS) and neonatal toxic shock syndrome-like exanthematous disease (NTED), in association with microbial virulence factors. The virulence factors produced by Staphylococcus aureus have a wide array of biological properties, including disruption of the epithelial barrier, inhibition of opsonization by antibody and complement, interference with neutrophil chemotaxis, cytolysis of neutrophils, and inactivation of antimicrobial peptides. Exfoliative toxins (ETs) induce the 'acantholytic' infection of S. aureus due to the disruption of cell-to-cell cohesion, which allows the pathogenic organisms to spread within the epithelium. Furthermore, S. aureus expresses exotoxins with biological properties of superantigens that induce T-cell activation with subsequent anergy and immunosuppression. Of the S. aureus leukotoxins, Panton-Valentine leukocidin (PVL) is involved in the development of multiple furuncles with more intense erythema, particularly in healthy young adults. TSS is an acute life-threatening illness caused by TSS toxin-1 (TSST-1) and is usually classified into two categories; menstrual TSS, originally described in association with tampon use, and nonmenstrual TSS with a variety of clinical settings. NTED is a neonatal disease induced by TSST-1 although clinical symptoms are much milder than those of TSS. In TSS and NTED, the expansion of TSST-1-reactive Vbeta2-positive T cells is observed. The production of pathogenic S. aureus exotoxins and biofilm formation is regulated by the accessory gene regulator (agr) locus in the quorum-sensing signaling pathway. There is no doubt that targeting the quorum-sensing signaling pathway or anti-toxin therapy is a promising therapeutic approach supportive of primary antibiotic therapy.

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  • Pathogenic link between hydroa vacciniforme and Epstein-Barr virus-associated hematologic disorders. International journal

    Keiji Iwatsuki, Masataka Satoh, Takenobu Yamamoto, Takashi Oono, Shin Morizane, Mikio Ohtsuka, Zi-Gang Xu, Daisuke Suzuki, Kazuhide Tsuji

    Archives of dermatology   142 ( 5 )   587 - 95   2006.5

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    OBJECTIVES: To determine the pathogenic association of latent Epstein-Barr virus (EBV) infections with both typical hydroa vacciniforme (HV) and severe HV-like eruptions, and to survey the complications and outcomes of patients. DESIGN: Case series. PATIENTS: Twenty-nine patients with HV or severe HV-like eruptions. INTERVENTIONS: In situ hybridization and immunostaining of biopsy specimens; extraction of DNA samples from cutaneous lesions and/or peripheral blood mononuclear cells for EBV DNA assay. MAIN OUTCOME MEASURES: Clinicopathologic manifestations, hematologic findings, complications, and outcomes; presence of latent EBV infection. RESULTS: T cells positive for EBV-encoded small nuclear RNA (EBER) were detected, to various degrees, in cutaneous infiltrates in 28 (97%) of 29 patients, including all 6 patients with definite HV with a positive phototest reaction, 11 of 12 patients with probable HV without evidence of photosensitivity, and all 11 patients with severe HV associated with systemic symptoms. In addition to EBER-positive T cells, many cytotoxic T lymphocytes expressing T-cell intracellular antigen 1 and granzyme B were present in the cutaneous lesions. Natural killer (NK) cells were absent or at a background level. The UV-induced cutaneous lesions showed histopathologic findings consistent with those of HV, containing many EBER-positive cells. Although no hematologic abnormalities were found in the definite and probable HV groups, the amounts of EBV DNA were increased in the peripheral blood mononuclear cells. By contrast, the severe HV group had markedly increased levels of EBV DNA associated with NK-cell lymphocytosis, and complications including chronic active EBV infection, hypersensitivity to mosquito bites, and hemophagocytic syndrome. Five patients with severe disease died of EBV-associated NK/T-cell lymphomas or hemophagocytic syndrome 2 to 14 years after onset. CONCLUSION: Both typical and severe HV are included within the spectrum of cutaneous disorders mediated by EBV-infected T cells, and the severe HV group may have overt EBV-associated NK/T-cell lymphoproliferative disorders with a frequently fatal outcome.

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  • Pemphigus vegetans with IgG and IgA antidesmoglein 3 antibodies. International journal

    S Morizane, T Yamamoto, Y Hisamatsu, K Tsuji, T Oono, T Hashimoto, K Iwatsuki

    The British journal of dermatology   153 ( 6 )   1236 - 7   2005.12

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  • The role of CD4 and CD8 cytotoxic T lymphocytes in the formation of viral vesicles

    S. Morizane, D. Suzuki, K. Tsuji, T. Oono, K. Iwatsuki

    British Journal of Dermatology   153 ( 5 )   981 - 986   2005.11

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    DOI: 10.1111/j.1365-2133.2005.06849.x

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  • Tendon destruction induced by Panton-Valentine leukocidin-positive Staphylococcus aureus in a patient with Sézary syndrome. Reviewed International journal

    O Yamasaki, S Morizane, H Akiyama, K Iwatsuki, J Kaneko, Y Kamio

    The British journal of dermatology   152 ( 3 )   586 - 7   2005.3

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    DOI: 10.1111/j.1365-2133.2005.06470.x

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  • The association between Staphylococcus aureus strains carrying panton-valentine leukocidin genes and the development of deep-seated follicular infection. Invited Reviewed International journal

    Osamu Yamasaki, Jun Kaneko, Shin Morizane, Hisanori Akiyama, Jirô Arata, Sachiko Narita, Jun-ichi Chiba, Yoshiyuki Kamio, Keiji Iwatsuki

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America   40 ( 3 )   381 - 5   2005.2

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    BACKGROUND: Panton-Valentine leukocidin (PVL) is mainly associated with necrotic suppurative lesions, such as furuncles and abscesses in the skin and subcutaneous tissue, but it has also been isolated from patients with community-acquired, severe, necrotizing pneumonia. However, the clinical manifestations of furuncles caused by PVL-producing Staphylococcus aureus and the role of patients' background are not fully understood. METHODS: We used polymerase chain reaction amplification to test for the PVL gene in 161 strains of S. aureus isolated from suppurative skin lesions. For all PVL gene-positive strains isolated from furuncles, we analyzed cutaneous manifestations, patient background characteristics, and bacteriological markers, including coagulase types, presence of the mecA gene, and toxin profiles, and we compared these results with those for PVL gene-negative strains. RESULTS: PVL genes were detected in 16 (40%) of the 40 S. aureus strains isolated from furuncles, 2 (28%) of the 7 strains isolated from carbuncles, 1 (14%) of the 7 strains isolated from abscesses, and 1 (5%) of the 20 strains isolated from folliculitis. PVL gene-positive S. aureus usually causes multiple (rather than single) furuncles, and such furuncles are usually associated with more-intense erythema around the lesions. PVL gene-positive strains were isolated from young adults without underlying diseases, whereas PVL gene-negative strains were isolated from patients with various systemic complications, including diabetes, leukemia, and autoimmune diseases. CONCLUSIONS: PVL gene-positive S. aureus strains are involved in the development of multiple furuncles with more-intense erythema, particularly in healthy young adults. An understanding of the characteristics of furuncles due to PVL gene-positive strains might be useful for preventing the development of the severe systemic infections.

    DOI: 10.1086/427290

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  • Involvement of granulysin-producing T cells in the development of superficial microbial folliculitis. Reviewed International journal

    T Oono, S Morizane, O Yamasaki, Y Shirafuji, W-K Huh, H Akiyama, K Iwatsuki

    The British journal of dermatology   150 ( 5 )   904 - 9   2004.5

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    BACKGROUND: Granulysin is a recently identified antimicrobial protein expressed on cytotoxic T cells, natural killer (NK) cells and NKT cells. It has been shown that granulysin contributes to the defence mechanisms against mycobacterial infection. Superficial microbial folliculitis is a common skin disease. In a previous report, we showed that, as a first line of defence, alpha-defensin, a human neutrophil peptide, and beta-defensin (human beta-defensin-2) were expressed in infiltrating neutrophils and in lesional epidermal keratinocytes, respectively, in superficial folliculitis. As we also observed many infiltrating lymphocytes in lesional dermis, we hypothesized that infiltrating lymphocytes may possess antimicrobial substances, such as granulysin, and play a role in the defence mechanism as a second line of defence. OBJECTIVES: Seven specimens of superficial microbial folliculitis diagnosed clinically and histologically were examined by means of immunohistochemistry. To identify the phenotype of cells expressing granulysin, confocal laser microscopic examination was performed. RESULTS: A dense lymphoid cell infiltrate was observed in pustules, in the perivascular regions. A large number of these lymphoid cells were positive for granulysin. Phenotypically, cells consisted of CD3+ T cells, CD8+ T cells and UCHL-1+ T cells. CD20+ cells and CD56+ cells were not observed. Microscopic examination with a confocal laser showed that the lymphocytes producing granulysin were CD3+ and CD4+ T cells but not CD8+ T cells. CONCLUSIONS: We showed that many granulysin-bearing T cells infiltrated affected follicles and perilesional dermis in superficial microbial folliculitis. However, few granulysin-positive lymphoid cells were observed in sterile pustular lesions. Our observations indicated that adaptive immunity such as granulysin, a lymphocyte-produced antimicrobial protein, may play an important role in the cutaneous defence mechanism.

    DOI: 10.1111/j.0007-0963.2004.05925.x

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  • Assessment of Streptococcus pyogenes microcolony formation in infected skin by confocal laser scanning microscopy. Reviewed International journal

    Hisanori Akiyama, Shin Morizane, Osamu Yamasaki, Takashi Oono, Keiji Iwatsuki

    Journal of dermatological science   32 ( 3 )   193 - 9   2003.9

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    BACKGROUND: Streptococcus pyogenes and Staphylococcus aureus are often simultaneously detected from many cases of non-bullous impetigo with atopic dermatitis. OBJECTIVES: Using confocal laser scanning microscopy (CLSM), to investigate formation of S. pyogenes microcolonies in skin lesions. METHODS: The S. pyogenes cells in the stationary growth phase alone were strongly stained with fluorescein isothiocyanate-concanavalin A (FITC-ConA), and this staining was reduced by pretreatment with amylase. Although the components of sugars in glycocalyx produced by S. pyogenes cells are unknown, we suggested that the materials stained by FITC-ConA were consistent with the presence of ConA-reactive sugars in glycocalyx produced by S. pyogenes cells. RESULTS: S. pyogenes cells associated with streptococcal impetigo skin and croton-oil inflamed mouse skin formed microcolonies encircled by materials (glycocalyx) that stained strongly with FITC-ConA, and these findings were consistent with those in biofilms. In croton-oil inflamed mouse skin, polymorphonuclear leukocytes (PMNs) infiltrated to just below the epidermis in the cefdinir-treated group but only to the middle dermis in the cefdinir-non-treated group. In this case S. pyogenes and S. aureus cells formed separate microcolonies and existed independently in the outer walls of pustule lesions of streptococcal impetigo. CONCLUSION: In skin infections, S. pyogenes and S. aureus formed aggregates of microcolonies (similar to that in biofilms) encircled by glycocalyx, which can make the infection hard to eradicate using an antimicrobial agent alone. The effect of conventional antimicrobial agents against biofilm is mainly due to the increase of the invasion of PMNs into the biofilm.

    DOI: 10.1016/S0923-1811(03)00096-3

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Books

  • Evolution of Atopic Dermatitis in the 21st Century

    Springer  2017 

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  • EBM皮膚疾患の治療up-to-date

    中外医学社  2015 

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  • 今日の処方改訂第5版「(共著)」

    南江堂  2013 

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  • 今日の治療と看護 改訂第3版

    南江堂  2013 

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  • 皮膚のリンパ腫 最新分類に基づく診療ガイド「(共著)」

    中山書店  2012 

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  • 皮膚疾患最新の治療2011-2012(共著)

    南江堂  2011 

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  • フィッツパトリック皮膚科学アトラス

    丸善株式会社  2008 

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MISC

  • 左膝に生じた限局型皮膚ノカルジア症

    澤井 希望, 立花 宏太, 川上 佳夫, 森実 真, 眞部 恵子, 芦田 日美野

    日本皮膚科学会雑誌   133 ( 8 )   1890 - 1890   2023.7

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  • 四肢と左臀部に皮下硬結を形成したExtensive Subcutaneous Sarcoidosisの1例

    臼井 真菜, 杉原 悟, 立花 宏太, 川上 佳夫, 森実 真, 中本 健太, 大塚 文男

    日本皮膚科学会雑誌   133 ( 8 )   1889 - 1889   2023.7

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  • 結節性多発性動脈炎に伴う難治性下腿潰瘍の原因としてステロイドによる創傷治癒遅延が考えられた1例

    浦上 仁志, 杉原 悟, 篠倉 美理, 森田 安理, 竹崎 大輝, 森実 真, 光井 聖子, 大橋 敬司

    日本皮膚科学会雑誌   133 ( 8 )   1889 - 1889   2023.7

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  • 粉瘤を発生母地とした臀部有棘細胞癌の1例

    蓮井 謙一, 立花 宏太, 川上 佳夫, 野村 隼人, 森実 真, 渡辺 敏之, 寺石 文則, 中井 友美

    日本皮膚科学会雑誌   133 ( 8 )   1893 - 1893   2023.7

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  • ニボルマブ投与後に尋常性乾癬と線状IgA水疱症(LABD)を発症した1例

    横溝 紗佑里, 三宅 智子, 川上 佳夫, 森実 真, 大橋 圭明, 古賀 浩嗣, 石井 文人

    日本皮膚科学会雑誌   133 ( 8 )   1893 - 1893   2023.7

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  • BNT162b2 mRNA COVID-19ワクチン接種後に発症したacute diffuse and total alopecia of the female scalpの1例

    川本 雅也, 川上 佳夫, 松田 吉弘, 平井 陽至, 森実 真, 多田 讓治

    日本皮膚科学会雑誌   133 ( 8 )   1891 - 1891   2023.7

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  • 脱毛から診断に至った高齢期発症の全身性エリテマトーデスの1例

    岡野 真理, 横山 恵美, 川上 佳夫, 森実 真, 勝山 恵理, 山口 春佳

    日本皮膚科学会雑誌   133 ( 8 )   1892 - 1892   2023.7

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  • 無症候性アミラーゼ血症に合併した皮下結節性脂肪壊死の1例

    松本 真理, 立花 宏太, 野村 隼人, 川上 佳夫, 森実 真, 岡田 梨乃, 石井 貴大, 山岡 主知, 浅川 知彦, 内田 治仁

    日本皮膚科学会雑誌   133 ( 8 )   1898 - 1898   2023.7

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  • 血管炎を伴う結節性紅斑様皮疹の後に,局面型サルコイドーシスを生じた1例

    赤松 由規, 横山 恵美, 川上 佳夫, 森実 真

    日本皮膚科学会雑誌   133 ( 8 )   1898 - 1898   2023.7

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  • ダブラフェニブ・トラメチニブ内服中にサルコイド様皮疹を呈した悪性黒色腫の1例

    別木 祐介, 神野 泰輔, 立花 宏太, 三宅 智子, 平井 陽至, 川上 佳夫, 森実 真

    日本皮膚科学会雑誌   133 ( 8 )   1894 - 1894   2023.7

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  • 透析患者に生じた後天性穿孔性皮膚症の2例

    岡野 真理, 立花 宏太, 川上 佳夫, 森実 真

    日本皮膚科学会雑誌   133 ( 8 )   1895 - 1895   2023.7

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  • 家族性黒色表皮腫に合併したと考えた菌状息肉症の1例

    立花 宏太, 長尾 僚祐, 川上 佳夫, 森実 真

    日本皮膚科学会雑誌   133 ( 8 )   1899 - 1899   2023.7

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  • 左前胸部に再発した皮膚原発粘液癌

    澤井 希望, 立花 宏太, 川上 佳夫, 森実 真, 杉原 悟

    日本皮膚科学会雑誌   133 ( 8 )   1899 - 1899   2023.7

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  • Open Treatmentを施行した内眼角部基底細胞癌の2例

    立花 宏太, 横溝 紗佑里, 蓮井 謙一, 神野 泰輔, 森実 真

    日本皮膚外科学会誌   27 ( 2 )   59 - 59   2023.7

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  • 口器除去後に残存したセメント物質により炎症が遷延したマダニ咬傷の1例

    佐藤 志帆, 川上 佳夫, 安富 陽平, 横山 恵美, 山崎 修, 森実 真, 吉岡 敏子

    日本皮膚科学会雑誌   133 ( 8 )   1885 - 1886   2023.7

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  • Gly m 4特異的IgEとプリックテストが陽性であった花粉-食物アレルギー症候群の1例

    森田 安理, 野村 隼人, 山崎 修, 森実 真, 高橋 祥子

    日本皮膚科学会雑誌   133 ( 8 )   1887 - 1887   2023.7

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  • 乳癌治療後に生じた左胸部放射線誘発性血管肉腫

    徳田 真優, 杉原 悟, 佐藤 志帆, 山口 春佳, 立花 宏太, 山崎 修, 森実 真, 高橋 正幸

    日本皮膚科学会雑誌   133 ( 8 )   1886 - 1887   2023.7

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  • 中等症劣性栄養障害型表皮水疱症に対して自家培養表皮移植を施行した1例

    竹崎 大輝, 三宅 智子, 瀧川 充希子, 野村 隼人, 山崎 修, 森実 真, 藤原 暖, 夏賀 健, 増地 裕

    日本皮膚科学会雑誌   133 ( 8 )   1887 - 1887   2023.7

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  • 鼠径リンパ節郭清後に発症したanesthesiamumpsの1例

    廣瀬 梓, 臼井 真菜, 篠倉 美理, 森田 安理, 杉原 悟, 山崎 修, 森実 真, 片岡 祐子, 松崎 孝

    日本皮膚科学会雑誌   133 ( 8 )   1886 - 1886   2023.7

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  • 骨折が判明した皮下深部解離性血腫の1例

    安富 陽平, 水田 康生, 芦田 日美野, 三宅 智子, 山崎 修, 森実 真

    日本皮膚科学会雑誌   133 ( 8 )   1888 - 1888   2023.7

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  • ブロダルマブの自己中断後に尋常性乾癬の膿疱化を生じた乾癬性関節炎の1例

    廣瀬 梓, 吉永 泰彦, 三宅 智子, 川上 佳夫, 森実 真

    日本皮膚科学会雑誌   133 ( 8 )   1888 - 1888   2023.7

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  • ラムシルマブ投与中に生じた多発性毛細血管拡張性肉芽腫の1例

    臼井 真菜, 横山 恵美, 野村 隼人, 山崎 修, 森実 真, 市原 英基

    日本皮膚科学会雑誌   133 ( 8 )   1887 - 1887   2023.7

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  • アトピー性皮膚炎における表皮バリア機能異常と外用療法

    森実 真

    西日本皮膚科   85 ( 3 )   219 - 219   2023.6

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  • LIPH遺伝子変異を認めた先天性縮毛症/乏毛症の兄妹例

    岡野 真理, 川上 佳夫, 森実 真, 鬼束 真美, 下村 裕

    西日本皮膚科   85 ( 3 )   231 - 231   2023.6

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  • 多彩なirAEを認めた左大腿原発悪性黒色腫症例

    神野 泰輔, 立花 宏太, 森実 真, 眞部 恵子

    日本皮膚悪性腫瘍学会学術大会プログラム・抄録集   39回   207 - 207   2023.6

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  • 岡山大学病院における乾癬診療Up-to-date 基礎の観点から考える乾癬診療

    森実 真

    日本皮膚科学会雑誌   133 ( 5 )   1328 - 1328   2023.5

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  • 非アルコール性脂肪肝炎は乾癬モデルマウスの皮膚IL-23A遺伝子発現を増強する

    竹崎 大輝, 向井 知之, 井関 將典, 橋口 太志, 白方 有香, 森実 真

    日本皮膚科学会雑誌   133 ( 5 )   1349 - 1349   2023.5

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  • 高齢者のセザリー症候群に対してインターフェロンγが奏功した1例

    澤井 希望, 三宅 智子, 徳田 真優, 佐藤 志帆, 立花 宏太, 杉本 佐江子, 川上 佳夫, 森実 真, 萩谷 英大, 淺田 騰

    日本皮膚科学会雑誌   133 ( 5 )   1411 - 1411   2023.5

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  • ラズベリーによるアナフィラキシーの1例

    赤松 由規, 川上 佳夫, 藤田 周作, 川本 友子, 三宅 智子, 平井 陽至, 森実 真

    日本臨床皮膚科医会雑誌   40 ( 3 )   465 - 465   2023.5

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  • インドール系メラニン中間代謝産物の血中濃度測定法の確立とメラノーマへの臨床応用

    梅村 啓史, 滝脇 正貴, 菊谷 善國, 福沢 世傑, 阿部 健太郎, 藤野 清孝, 中山 智祥, 森実 真, 佐藤 守, 山崎 修

    日本皮膚科学会雑誌   133 ( 5 )   1359 - 1359   2023.5

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  • 当院における強皮症合併重症下肢虚血7例の臨床学的検討

    松田 吉弘, 三宅 智子, 戸田 洋伸, 立花 宏太, 野村 隼人, 平井 陽至, 川上 佳夫, 迫田 直也, 廣田 真規, 森実 真

    日本皮膚科学会雑誌   133 ( 5 )   1361 - 1361   2023.5

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  • 桂枝茯苓丸を併用した酒さ様皮膚炎の3例

    高須賀 琴巳, 横溝 紗佑里, 横山 恵美, 川上 佳夫, 安井 陽子, 神崎 寛子, 森実 真

    日本臨床皮膚科医会雑誌   40 ( 3 )   480 - 480   2023.5

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  • 初診時,手足口病様皮疹を呈した新生児水疱性類天疱瘡の1例

    別木 祐介, 徳田 真優, 前 琴絵, 妹尾 春佳, 立花 宏太, 三宅 智子, 平井 陽至, 川上 佳夫, 森実 真, 宇田 和宏, 斎藤 友希恵, 茂原 研司, 津下 充, 八代 将登

    日本皮膚科学会雑誌   133 ( 4 )   715 - 715   2023.4

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  • セクキヌマブが奏功した掌蹠膿疱症を背景とする膿疱性乾癬の1例

    澤井 希望, 川上 佳夫, 徳田 真優, 廣瀬 梓, 別木 祐介, 高須賀 琴巳, 篠倉 美里, 立花 宏太, 横山 恵美, 三宅 智子, 平井 陽至, 森実 真, 多田 譲治

    日本皮膚科学会雑誌   133 ( 3 )   524 - 524   2023.3

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  • 逆行性顔面動脈皮弁を用いて再建した上口唇有棘細胞癌の1例

    杉原 悟, 立花 宏太, 森実 真, 山崎 修

    日本皮膚科学会雑誌   133 ( 2 )   266 - 266   2023.2

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  • 休止期脱毛と爪甲剥離を呈したCronkhite-Canada症候群の1例

    横溝 紗佑里, 野村 隼人, 中川 裕貴, 森実 真, 衣笠 秀明

    日本皮膚科学会雑誌   133 ( 2 )   255 - 255   2023.2

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  • 新規ALOX12B遺伝子病的バリアントを認めた先天性魚鱗癬様紅皮症の1例

    前 琴絵, 梶田 藍, 川上 佳夫, 森実 真, 野田 達宏, 武市 拓也, 秋山 真志

    日本皮膚科学会雑誌   133 ( 2 )   262 - 262   2023.2

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  • Three cases of adult T-cell leukemia with graft-versus-host disease worsened after the mogamulizumab administration

    横山恵美, 山崎修, 山崎修, 淺田騰, 吉野正, 森実真

    皮膚病診療   44 ( 5 )   2022

  • カルシウム製剤の血管外漏出による医原性石灰沈着症の2例

    臼井真菜, 山崎修, 赤松由規, 森田安理, 杉原悟, 三宅智子, 横山恵美, 森実真

    皮膚科の臨床   64 ( 11 )   2022

  • 日本人菌状息肉症患者におけるベキサロテン内服の効果と安全性 岡山大学での投薬症例による解析

    中川 裕貴, 濱田 利久, 平井 陽至, 三宅 智子, 山崎 修, 岩月 啓氏, 森実 真

    西日本皮膚科   83 ( 6 )   549 - 553   2021.12

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    ベキサロテンは本邦で2016年に皮膚T細胞リンパ腫(CTCL)に対し承認され,現在ではCTCL治療に広く用いられている。欧米ではベキサロテン長期投与症例が報告されているが,日本ではまだ承認から日が浅いため長期投与症例の報告は少ない。当院でベキサロテンにて加療された11例の菌状息肉症(MF)患者について解析した。その中で長期に渡りベキサロテンを投与され良好な経過を示した3例を報告する。甲状腺機能低下症と脂質異常症はベキサロテン関連の副作用として頻発し,当院でも11例全例にみられたが,重篤な副作用はみられなかった。長期投与症例ではベキサロテンの減量や一時中止,副作用に対する内服投与にてベキサロテンを長期継続することができた。3例とも治療反応性はよく,2例がCR,1例がPRを得た。ベキサロテンは日本人MF患者においても副作用をコントロールすることで長期に投与でき,有効な治療選択肢となりうる。(著者抄録)

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  • 長期生存を得た肝転移乳房外Paget病の1例

    内藤 聖子, 神野 泰輔, 山崎 修, 森実 真, 野村 隼人, 河田 真理子, 吉富 惠美, 荒川 謙三

    西日本皮膚科   83 ( 5 )   431 - 435   2021.10

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  • トラベクテジン血管外漏出による皮膚壊死2例の治療経験

    杉原 悟, 池田 賢太, 山崎 修, 森実 真, 下江 敬生, 中村 圭一郎

    西日本皮膚科   83 ( 5 )   417 - 422   2021.10

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    症例1:72歳,女性。子宮平滑筋肉腫の多発肺転移に対して投与されたトラベクテジンの血管外漏出があり,右胸部CVポート刺入部を中心に広範囲の組織壊死を来した。適宜デブリドマンを施行し,4×8cmの範囲に全層植皮術を行い漏出後71日目に略治を得た。症例2:67歳,女性。子宮平滑筋肉腫の多発リンパ節転移,肺転移に対して投与されたトラベクテジンの血管外漏出があり,右胸部CVポート刺入部を中心に広範囲の組織壊死を来した。適宜デブリドマンを施行し,5×5cmの範囲に全層植皮術を行い漏出後96日目に略治を得た。紅斑部の病理所見では,いずれも表皮,真皮には炎症所見は目立たず,皮下組織の広範囲な壊死があった。表皮・真皮の温存ができると考え,皮下組織のみのデブリドマンによって植皮範囲を縮小できた。トラベクテジンの血管外漏出に遭遇した際は感染やさらなる壊死の拡大を防ぎ,創部の縮小を目指して加療することが必要である。また医原性の障害であり,自験例のように数回のデブリドマンと植皮術が必要で,傷閉鎖までは長期間を要することを慎重に説明していくことが重要である。(著者抄録)

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  • Melanosis of the Nipple and Areolaの1例

    内藤 聖子, 松田 真由子, 馬屋原 孝恒, 山崎 修, 森実 真

    皮膚科の臨床   63 ( 11 )   1752 - 1753   2021.10

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    29歳女性。右乳頭の黒色斑の精査目的で当科へ紹介となった。所見では右乳頭部に8×5mmのやや不整形な黒褐色斑が認められた。生検を行ったところ、melanosis of the nipple and areolaと診断され、患者と相談の上、整容的観点から切除せず、経過観察とした。

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  • 臨床講義 乾癬の病態理解の進歩と最新の治療

    森実 真

    皮膚科の臨床   63 ( 9 )   1365 - 1371   2021.8

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    <文献概要>乾癬に関する研究は世界中で精力的に行われており,病態の理解も飛躍的に進歩している。その目覚ましい研究の成果が臨床応用され,乾癬に対する新しい薬剤や治療法が毎年のように登場している。本稿では乾癬の病態理解の進歩と最新の治療について詳説する。

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  • 【診察室での患者さんの質問に答えます】扁平苔癬 原因はなんですか 口腔粘膜

    森実 真

    皮膚科の臨床   63 ( 6 )   876 - 877   2021.6

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  • 【好酸球が関与する皮膚病】好酸球浸潤が著明であったPanton-Valentine leukocidin陽性MRSAによるせつ腫症

    小橋 美那, 橋本 倫子, 三宅 智子, 山崎 修, 森実 真

    皮膚病診療   43 ( 6 )   524 - 527   2021.6

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    <文献概要>症例のポイント ・健常人のPanton-Valentine leukocidin(PVL)産生の市中型MRSA(methicillin-resistant Staphylococcus aureus)感染によるせつ腫症であり,再発を繰り返し治療に難渋した.・せつ,せつ腫症の典型の病理組織像では毛包周囲の壊死,好中球の浸潤を認めるが,著明な好酸球の浸潤を認めた.

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  • ハンセン病患者に生じた有棘細胞癌

    安富 陽平, 山崎 修, 立花 宏太, 杉本 佐江子, 森実 真

    Skin Cancer   36 ( 1 )   71 - 75   2021.6

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    91歳,男性。既往にハンセン病,尿管癌がある。ADLが悪く,ほぼ寝たきり状態。2年前に顔面神経麻痺のある左頬にびらんと痂皮が散在し,日光角化症として液体窒素などで加療されていたが,腫瘤になり徐々に増大し,生検で有棘細胞癌と診断された。他院では手術や放射線治療が検討されたが保存的治療の方針となっていた。創部を触る動作が増えたためアセトアミノフェン内服,潰瘍面より出血がありモーズ療法を行っていた。その後さらに腫瘤は増大し,当科を紹介受診した。左頬に中央に潰瘍を伴う12×10cmの淡紅褐色,広基性の腫瘤があった。CTではリンパ節,遠隔転移は認めなかった。超高齢でADLが悪く,低アルブミン血症,胸水があり,腫瘤が大きく輸血が必要でハイリスク症例であった。局所制御目的で全身麻酔下に切除,植皮を行った。しかし術後5ヵ月後に肺炎で永眠された。(著者抄録)

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    Other Link: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2021&ichushi_jid=J02197&link_issn=&doc_id=20210719270014&doc_link_id=10.5227%2Fskincancer.36.71&url=https%3A%2F%2Fdoi.org%2F10.5227%2Fskincancer.36.71&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_2.gif

  • 乾癬の生物学的製剤治療に対する結核対策実態の多施設共同調査

    金子 栄, 鶴田 紀子, 伊藤 宏太郎, 山口 和記, 宮城 拓也, 高橋 健造, 東 裕子, 森実 真, 野村 隼人, 山口 道也, 日野 亮介, 澤田 雄宇, 中村 元信, 大山 文悟, 大畑 千佳, 米倉 健太郎, 林 宏明, 柳瀬 哲至, 松阪 由紀, 杉田 和成, 菊池 智子, 三苫 千景, 中原 剛士, 古江 増隆, 岡崎 布佐子, 小池 雄太, 今福 信一, 西日本炎症性皮膚疾患研究会

    日本皮膚科学会雑誌   131 ( 6 )   1525 - 1532   2021.5

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    乾癬治療における生物学的製剤使用時の結核スクリーニングの現状について西日本の18施設を調査した。事前の検査ではinterferon gamma release assay(IGRA)が全施設で行われ、画像検査はCTが15施設、胸部レントゲンが3施設であった。フォローアップでは検査の結果や画像所見により頻度が異なっていた。全患者1,117例のうち、IGRA陽性で抗結核薬を投与されていた例は64例、IGRA陰性で抗結核薬を投与されていた例は103例であり、副作用を認めた患者は23例15%であった。これらの適切な検査と治療により、結核の発生頻度が低く抑えられていると考えられた。(著者抄録)

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  • CVポート部より漏出したトラベクテジンによる広範囲壊死の2例

    杉原 悟, 池田 賢太, 山崎 修, 森実 真, 下江 敬生, 中村 圭一郎

    日本皮膚外科学会誌   25   54 - 55   2021.5

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  • 口腔扁平苔癬から発症し、歯科金属除去後に毛孔性扁平苔癬を合併した1例

    横山 恵美, 木村 摩耶, 山崎 修, 森実 真, 山内 晶子, 濱田 利久, 佐々木 朗

    西日本皮膚科   83 ( 2 )   120 - 124   2021.4

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    68歳,女性。半年前に舌痛および口腔内違和感が生じ,口腔外科にて口腔扁平苔癬と診断され,その後下腿に暗紅色の紅斑が出現した。金属パッチテストで,亜鉛陽性となり,該当歯科金属を除去したところ,数日後より頭皮にそう痒を伴う脱毛が多発した。初診時,頭部に淡紅色の紅斑を伴う脱毛斑が9ヶ所みられ,病理組織学的に毛包漏斗部および峡部上皮のリンパ球浸潤と周囲間質の線維化および正常毛球の残存を認めた。下腿紅斑は表皮真皮境界部の空胞変性と真皮の帯状リンパ球浸潤の像であった。脱毛斑に対しステロイド局注を開始し,約2ヵ月後にそう痒と紅斑は改善し,約8ヵ月後に,小型の脱毛斑2ヶ所は治癒した。その後脱毛瘢痕部にエキシマライトを約1.5年間照射し脱毛斑はさらに3ヶ所治癒した。口腔粘膜部のびらんや下腿紅斑は出没を繰り返し,初診より1.5年後,腟周囲にびらんおよび白色局面が出現し,いずれもステロイド外用を継続中である。自験例は歯科金属除去後に扁平苔癬の一部として毛包性扁平苔癬が生じ,既存の口腔や下腿病変の増悪と腟周囲にも病変が生じた経過が特徴的であった。当院で過去2年間のうち金属パッチテスト陽性の扁平苔癬は15例あり,年齢は39歳から84歳までの男性4名,女性11名で,粘膜型13例,皮膚型1例,皮膚粘膜毛孔性扁平苔癬型1例(自験例)であった。該当金属を除去したのは4例で,経過不明の2例をのぞき,自験例を含めいずれも症状は改善しなかった。(著者抄録)

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  • らい性結節性紅斑が慢性化したLL型ハンセン病

    篠倉 美理, 尾崎 元昭, 川上 佳夫, 梅川 康弘, 吉原 由樹, 石井 則久, 山崎 修, 森実 真

    日本皮膚科学会雑誌   131 ( 2 )   329 - 335   2021.2

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    20歳代の在日外国人男性。右手疼痛を主訴に整形外科を受診した際に皮疹を指摘され、当科を受診した。顔面・四肢末梢・腰腹部に光沢のある境界明瞭な結節を多数認め、臨床症状、皮膚スメア検査、組織所見、らい菌特異的PCR陽性よりLL型ハンセン病と診断した。ハンセン病およびらい反応は、治療が遅れると後遺症を残しうるため早期診断・早期治療が重要である。自験例は多剤併用療法開始後に皮膚症状の悪化や神経・眼症状、発熱、関節痛など多彩ならい性結節性紅斑の症状が出現し、その後慢性化したが、ほとんど後遺症なく経過している。(著者抄録)

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  • 臭化カリウム減量により症状が軽快した臭素疹の1例

    安富 陽平, 川上 佳夫, 篠倉 美理, 平井 陽至, 山崎 修, 森実 真

    西日本皮膚科   83 ( 1 )   18 - 21   2021.2

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    1歳,女児。乳児早期発症てんかんで治療中であったが,けいれん発作のコントロールが不良で臭化カリウムの内服を開始した。内服開始2ヵ月半後に四肢に紅色丘疹が出現し,膿疱を伴う紅色局面に進展した。病理組織では表皮内に好中球の浸潤,真皮では全層にわたって膠原線維束に分け入るような好中球の浸潤があり,とくに毛包周囲や毛包上皮への好中球の浸潤が顕著であった。血中臭化物濃度は91.8mg/dlと高値であり,臭素疹と診断した。臭化カリウムを減量してステロイド軟膏の外用で皮疹は軽快した。内服減量により皮疹が軽快したことから,臭素疹の機序はアレルギー性ではなく,用量依存性に発症する機序が推測される。(著者抄録)

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  • Spitz母斑と黒子様色素細胞母斑の隣接発生例 免疫染色によるROS1過剰発現とBRAF V600E変異蛋白の証明

    山下 珠代, 山崎 修, 高田 実, 松浦 能子, 谷口 恒平, 後藤 啓介, 森実 真

    西日本皮膚科   83 ( 1 )   30 - 33   2021.2

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    25歳,女性。初診の約6ヵ月前より右上腕に紅色結節が出現し,急速に増大した。肉眼所見では5×10mmの境界明瞭な紅色扁平結節が存在しそれと隣接して2mmの黒褐色の小色素斑が認められた。病理組織学的に結節部はSpitz母斑,小色素斑は黒子様色素細胞母斑であった。さらに,免疫組織化学的に結節部にはROS1蛋白の過剰発現が,色素斑にはBRAF V600E変異蛋白が検出され,両者は発生機序の異なる病変であることが示された。(著者抄録)

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  • 瀬戸内海に生息するナルトビエイによる外傷後に生じたMycobacterium marinum感染症の1例

    谷本 尚吾, 川上 佳夫, 安富 陽平, 山崎 修, 森実 真

    西日本皮膚科   82 ( 6 )   460 - 463   2020.12

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    71歳,男性。2018年8月上旬に,瀬戸内海での漁で網にかかったナルトビエイ(Aetobatus narutobiei)を外していた時に,尻尾の棘が左小指の基部に刺さった。直後に海水で洗浄し,しばらく自宅で様子をみていたが,同部位が次第に腫脹してきたため,1ヵ月半後に当科を受診した。初診時,左小指MP関節の背側に中心がクレーター状に陥凹し,潰瘍化している15×14mmの紅色結節を認めた。組織片を2%小川培地に接種し,30℃と35℃の条件下で培養を行ったところ,いずれの条件下でも28日目に灰白色のコロニー形成を認め,MALDI-TOF MSのマススペクトルのパターンからMycobacterium marinumと診断した。皮膚病変はミノサイクリン塩酸塩(200mg/日内服を3ヵ月間)を投与して完治した。エイ刺傷は,その鋭利な尾棘による損傷と刺毒による炎症を特徴とし,創部感染のリスクもある。近年ナルトビエイの瀬戸内海での生息域の拡大が問題視されている。そのため,エイ刺傷への対応を知ることは大切である。(著者抄録)

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  • 【抗菌ペプチドとマイクロバイオーム】オーバービュー 抗菌ペプチドとマイクロバイオーム

    森実 真

    皮膚アレルギーフロンティア   18 ( 3 )   127 - 130   2020.11

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    微生物の侵入から身体を守る皮膚のバリア機構においては、「抗菌ペプチド」が重要な役割を果たしている。抗菌ペプチドは広域の抗菌活性をもつのみならず、炎症誘導、創傷治癒促進、血管新生促進など、さまざまな機能を有していることも報告され、アラーミン(alarmin)の一種とも考えられている。一方、皮膚のバリア形成には、その場に存在する常在菌も重要な役割を果たしていることがわかってきた。常在菌叢を構成する個々の細菌種のゲノムの集合体を「マイクロバイオーム」と呼ぶ。近年のシークエンス技術の発展によって、正常皮膚および皮膚疾患病変部のマイクロバイオームに関しても、さまざまな新知見が得られている。(著者抄録)

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  • カセリサイディンLL-37の真の役割は?

    森実 真, 山崎 研志

    皮膚アレルギーフロンティア   18 ( 3 )   154 - 161   2020.11

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  • ボリコナゾール長期内服中に発症した有棘細胞癌と多発日光角化症の1例

    篠倉 美理, 光井 聖子, 山崎 修, 森実 真

    臨床皮膚科   74 ( 11 )   887 - 891   2020.10

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    <文献概要>73歳,男性.4年前に肺アスペルギルス症の治療のためボリコナゾールを内服開始した.2年前から頭部,顔面,両手背に角化性の丘疹,紅斑が多発した.病理組織所見は,顔面は有棘細胞癌,頭部・手背は日光角化症であった.ボリコナゾール内服中止とイミキモド外用により皮疹は改善した.ボリコナゾールは,その代謝物が紫外線を吸収して活性酸素を産生し,表皮のDNA損傷を惹起する.さらにボリコナゾールそのものがCOX-2の転写活性を誘導することで発癌を促進させる可能性が示されており,長期内服中に光線過敏症状を経て日光角化症,有棘細胞癌を生じうる.ボリコナゾールの光線関連皮膚障害は本邦ではまだ報告が少ないものの,十分な遮光指導を受けていない肺アスペルギルス症の患者に多い.ボリコナゾール内服患者には具体的な遮光指導と慎重な皮膚症状の経過観察を行うことで,光線関連皮膚障害を最小限に抑えることが期待できる.

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  • 【乾癬は奥深い】日光角化症に対するイミキモドクリームで誘発された乾癬

    妹尾 春佳, 山崎 修, 森実 真, 辻 和英

    皮膚病診療   42 ( 9 )   758 - 761   2020.9

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    <文献概要>症例のポイント ・イミキモドは単球および形質細胞様樹状細胞を主体としたTLR7に作用し,その結果IL-17/IL-23の産生を促し,乾癬のマウスモデルとして使用されている.・日光角化症の患者に対するイミキモドクリーム外用により,外用部位以外にも角化性紅斑が出現し,乾癬が誘発された.・患者は乾癬の既往はなかったが,イミキモドクリームにより強い紅斑反応を示していた.

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  • セツキシマブ・パクリタキセル併用療法中に生じたLeukocytoclastic Vasculitisの1例

    安富 陽平, 川上 佳夫, 篠倉 美理, 神野 泰輔, 藤本 裕子, 三宅 智子, 山崎 修, 森実 真

    西日本皮膚科   82 ( 4 )   271 - 275   2020.8

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    58歳,男性。右外耳道有棘細胞癌に対してepidermal growth factor receptor(EGFR)阻害剤のセツキシマブとパクリタキセルの併用療法を6ヵ月間,合計14回施行した。最終投与日から13日後に四肢に一部血疱や浸潤を伴う点状紫斑が多発し,腎機能障害も認めた。皮膚病理所見では真皮全層の血管周囲に好中球や好酸球の浸潤と核塵を認め,真皮浅層から中層の毛細血管に血管壁のフィブリノイド変性があり,leukocytoclastic vasculitis(LCV)に矛盾しない所見であった。蛍光抗体直接法では真皮上層の血管周囲にC3の沈着が認められたが,IgAの沈着は認めなかった。セツキシマブ・パクリタキセルを休薬し,プレドニゾロン(PSL)20mg/日内服と細胞外液の補液を開始したところ,皮疹,腎機能ともに速やかに改善した。しかし,蛋白尿と尿潜血が持続して検出されたため,PSL8.0mg/日を維持量として継続した。その後外耳道癌が再び増大し,4ヵ月後にセツキシマブ・パクリタキセルを再投与したが皮疹の再燃は認めなかった。EGFR阻害剤投与中に,血管周囲にC3の沈着を伴うLCVの報告が本症例以外にも3例ある。そのうち1例では潰瘍化や壊死を伴う臨床像を呈し,1例では下血を合併していた。本症例ではEGFR阻害剤がLCV発症の直接の誘因になったかどうかは不明だが,EGFR阻害剤によりLCVが重症化した可能性が考えられる。(著者抄録)

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  • 私の診療経験から 当科におけるアトピー性皮膚炎診療・研究 表皮セリンプロテアーゼ活性について

    森実 真

    臨牀と研究   97 ( 8 )   1042 - 1046   2020.8

  • アトピー性皮膚炎と表皮セリンプロテアーゼ活性

    森実 真

    西日本皮膚科   82 ( 3 )   213 - 214   2020.6

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  • 臨床研究 患者への情報提供を目的とした頭部血管肉腫に対する化学放射線療法の有害事象の検討

    黒田 桂子, 山崎 修, 三宅 智子, 加持 達弥, 岩月 啓氏, 森実 真

    皮膚科の臨床   62 ( 4 )   445 - 448   2020.4

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    <文献概要>頭部血管肉腫に対して,放射線療法とタキサン系抗癌剤による化学放射線療法が主流となっており,有害事象や合併症により入院が長期化することがある。しかしながら,高齢の患者が治療について十分に理解できていないことも多く,治療のコンプライアンス低下につながる。そこで患者への正確な情報提供を目的として,頭部血管肉腫の入院中の有害事象について検討した。入院期間は中央値57日,化学放射線療法による有害事象は放射線皮膚炎94%,皮膚びらんからの感染41%,脱毛53%,血球減少53%であり,発現時期と期間を明確にできた。事前に主なスケジュールと有害事象について十分説明することが重要である。

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  • 【アレルギー疾患の最新の治療】最新のアレルギー疾患治療 薬疹

    森実 真

    臨牀と研究   97 ( 3 )   309 - 314   2020.3

  • 西日本豪雨災害における皮膚科診療支援と岡山県下皮膚科の災害医療対策について

    辻 和英, 神崎 寛子, 服部 浩明, 森実 真, 青山 裕美, 林 宏明, 佐藤 淳

    日本臨床皮膚科医会雑誌   36 ( 6 )   724 - 730   2019.11

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  • 免疫チェックポイント阻害薬を投与した多発性筋炎合併悪性黒色腫の1例

    野村 隼人, 山崎 修, 加持 達弥, 若林 宏, 宮脇 義亜, 森実 真

    西日本皮膚科   81 ( 5 )   396 - 400   2019.10

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    61歳,男性。2013年5月に多発性筋炎と診断され,プレドニゾロン(PSL)で治療が開始された。治療開始前のCTで左頸部と左腋窩リンパ節腫脹を認め経過観察されていたが,リンパ節は増大傾向を呈し,リンパ節生検で悪性黒色腫と診断された。精査では原発巣は認められなかった。DTIC療法とDAC-Tam-Feron療法を受けたが効果に乏しく,2015年3月からベムラフェニブを開始し転移巣は速やかに縮小したが,転移巣が増大してきたため,2015年11月にニボルマブ2mg/kgを3週間間隔で投与開始した。ニボルマブ2回目投与後から筋原性酵素の上昇や疲労感を認め,多発性筋炎の増悪と考えた。ニボルマブを継続しつつ,PSLを5mgから20mgに増量したところ,筋症状は改善した。しかし転移巣は増大傾向にあったため,ニボルマブは5回で中止した。イピリムマブも効果がなく,2016年6月からダブラフェニブとトラメチニブの併用療法を開始した。転移巣は速やかに縮小したが,次第に耐性を示すようになった。2017年7月に多発転移により全身状態が悪化し,永眠した。免疫チェックポイント阻害薬により既存の自己免疫疾患が悪化したが,自己免疫疾患悪化時も適切な対応を行うことにより免疫チェックポイント阻害薬は継続可能であった。(著者抄録)

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  • 【肉芽腫症】皮疹から診断された全身リンパ節腫脹をきたしたサルコイドーシスの1例

    森田 安理, 川上 佳夫, 三宅 智子, 平井 陽至, 加持 達弥, 山崎 修, 森実 真

    皮膚科の臨床   61 ( 11 )   1611 - 1614   2019.10

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    <文献概要>65歳,女性。4年前より左耳前部に紅斑が出現した。近医皮膚科でステロイド外用を開始されるも難治であった。生検で真皮内に類上皮細胞肉芽腫を認め,サルコイドーシスの疑いで精査・加療目的に当科を紹介受診した。初診時,左耳前部に鱗屑を付す4×5cm大の円板状紅斑,耳介部に潰瘍形成,頭頂部に15×20mm大の瘢痕様紅斑を認めた。単純CTで両側縦隔リンパ節に加え,両鼠径リンパ節など全身のリンパ節腫脹を認めた。皮膚生検,鼠径リンパ節生検,気管支鏡検査でいずれも非乾酪性肉芽腫を認めたため,サルコイドーシスと確定診断した。自験例は感染症との鑑別を要する非典型的臨床像であったが,鼠径リンパ節生検により診断を裏づけることができた。また,Th2優位に移行した成熟期のサルコイドーシスであったと推測する。

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  • アトピー性皮膚炎とカリクレイン関連ペプチダーゼ

    森実 真

    西日本皮膚科   81 ( 5 )   367 - 371   2019.10

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    表皮のバリア形成に重要な役割を果たす表皮角化細胞産生カリクレイン関連ペプチダーゼとアトピー性皮膚炎(AD)との関連性について述べた。多くのカリクレイン(KLKs)はAD病変部において発現が増強されており、表皮バリア機能を低下させることによって病態形成と関連している可能性がある。表皮角化細胞はKLKsの阻害因子であるリンパ上皮Kazal型関連阻害因子(LEKTI)、さらにSLPI、PI3、SPINK6、SPINK9なども発現するため、AD病変部の過剰なプロテアーゼ活性については酵素のみならず、阻害因子の発現量および機能、さらにはpHの変化も含めて総合的に酵素活性増強機構を検討する必要がある。

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  • 免疫チェックポイント阻害薬を投与した多発性筋炎合併悪性黒色腫の1例

    野村 隼人, 山崎 修, 加持 達弥, 若林 宏, 宮脇 義亜, 森実 真

    西日本皮膚科   81 ( 5 )   396 - 400   2019.10

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  • 各診療科の協力で切除しえた宗教的輸血拒否患者の巨大びまん性神経線維腫

    立花 宏太, 山崎 修, 光井 聖子, 山下 珠代, 岩月 啓氏, 森実 真, 西森 久和, 生口 俊浩, 坂野 彩, 谷口 新, 小林 求, 森田 幸子, 橋本 倫子

    日本皮膚外科学会誌   23 ( 2 )   130 - 131   2019.9

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  • 各診療科の協力で切除しえた宗教的輸血拒否患者の巨大びまん性神経線維腫

    立花 宏太, 山崎 修, 光井 聖子, 山下 珠代, 岩月 啓氏, 森実 真, 西森 久和, 生口 俊浩, 坂野 彩, 谷口 新, 小林 求, 森田 幸子, 橋本 倫子

    日本皮膚外科学会誌   23 ( 2 )   130 - 131   2019.9

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  • 同時多発性悪性黒色腫の1例

    池田 賢太, 浦上 仁志, 杉原 悟, 加持 達弥, 山崎 修, 森実 真, 増地 裕

    日本皮膚外科学会誌   23 ( 2別冊 )   211 - 211   2019.9

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  • 【帯状疱疹の新時代-診療の基本と最新情報-】(Part2)気になる帯状疱疹のサイン ヘルペス型水疱における中心臍窩形成のメカニズム

    森実 真

    Visual Dermatology   18 ( 9 )   906 - 906   2019.8

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  • 有茎広背筋皮弁で再建した冠動脈ステント留置後の右肩甲部放射線性皮膚潰瘍

    森田 安理, 石井 芙美, 川上 佳夫, 加持 達弥, 竹原 彩, 大塚 正樹, 山崎 修, 森実 真

    皮膚科の臨床   61 ( 9 )   1387 - 1390   2019.8

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    <文献概要>75歳,男性。狭心症で冠動脈ステント留置後3ヵ月目頃より右肩甲部に痒みを伴う紅斑が出現し,1年後に潰瘍化した。デブリードマンと外用療法で約1年半加療されるも難治であったため,精査・加療目的に当科を紹介受診した。初診時,右肩甲部に3×3cm大の深い潰瘍があり周囲8×7cm大の範囲に板状硬結,暗褐色色素沈着と血管拡張を認めた。MRIでは筋層まで達していた。冠動脈造影による放射線性皮膚潰瘍と考え,潰瘍,硬結を切除,有茎広背筋皮弁術を施行した。術後は機能面,整容面ともに良好であった。右肩甲部皮膚潰瘍の患者に遭遇した場合,冠動脈造影の既往を問診することが重要である。また再建方法としては有茎広背筋皮弁術が治療選択肢となる。

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  • 【間葉系腫瘍】下口唇に生じたSolitary Fibrous Tumorの1例

    香曽我部, 純子, 川上 佳夫, 光井 聖子, 三宅 智子, 山崎 修, 岩月 啓氏, 小田 和歌子, 森実 真

    皮膚科の臨床   61 ( 8 )   1195 - 1198   2019.7

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    <文献概要>52歳,男性。2年前より下口唇の口腔粘膜部に自覚症状のない粘膜下結節が出現した。超音波検査では,境界明瞭な10mm大の粘膜下結節を認め,内部不均一な低エコー像を示した。カラードプラ像では豊富な血流信号を認めた。結節を全摘出したところ,病理組織では異型に乏しい長紡錘形細胞が特定の配列をとることなく増殖し,間質に不規則に分枝した鹿角様血管を認めた。免疫染色ではCD34とbcl-2がびまん性に陽性で,CD31とfactorVIIIは陰性であった。また,抗STAT6抗体による免疫染色では,核に陽性であった。以上の所見からsolitary fibrous tumor(SFT)と診断した。臨床所見ではmucous cystとの鑑別を要したが,超音波検査で血流を確認することがSFTの診断の一助となると考えた。

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  • ボリコナゾール長期内服中に発症した多発日光角化症

    篠倉 美理, 山崎 修, 森実 真, 光井 聖子

    日本皮膚科学会雑誌   129 ( 8 )   1653 - 1653   2019.7

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  • 腋窩リンパ節転移後、長期経過観察していた背部悪性黒色腫

    安富 陽平, 山崎 修, 神野 泰輔, 加持 達弥, 森実 真, 浅越 健治, 荒川 謙三

    日本皮膚科学会雑誌   129 ( 8 )   1653 - 1653   2019.7

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  • 骨転移、右肺門部転移を認めた基底細胞癌の1例

    浦上 仁志, 加持 達弥, 山崎 修, 森実 真, 山田 潔, 黒住 和彦, 国定 俊之, 田端 雅弘

    日本皮膚科学会雑誌   129 ( 8 )   1653 - 1653   2019.7

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  • 各診療科の協力で切除し得た宗教的輸血拒否患者のびまん性神経線維腫

    立花 宏太, 山崎 修, 光井 聖子, 山下 珠代, 岩月 啓氏, 森実 真, 西森 久和, 生口 俊浩, 坂野 彩, 谷口 新, 小林 求, 森田 幸子, 橋本 倫子

    皮膚科の臨床   61 ( 8 )   1303 - 1306   2019.7

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    <文献概要>69歳,女性。神経線維腫1型に伴うびまん性神経線維腫と特発性血小板減少性紫斑病(ITP)を合併。宗教的輸血拒否患者。25年前より右下腹部の腫瘤が徐々に増大してきていた。2016年当科を初診し,手術を計画したが,宗教上の輸血療法不能を理由に中止となった。2017年腫瘤の増大と表面の紫斑が出現した。腫瘤表面に拳大の紫斑と内部に硬結を触知,貧血の進行を認め,腫瘤内出血が疑われ緊急入院した。医療安全上の対応と周術期の対策を各科・各部署の協力で検討した。ITPに対してプレドニゾロン内服,術前に動脈塞栓術を施行後,術中の閉鎖回路による希釈式自己血灌流のうえ,術中出血は200mlで腫瘍を切除できた。院内の医療連携システムの充実が重要である。

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  • ニボルマブが奏効した食道悪性黒色腫の2例

    加持 達弥, 山崎 修, 森実 真, 岩月 啓氏, 前田 直見, 白川 靖博

    Skin Cancer   34 ( 1 )   35 - 40   2019.6

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    症例1:60歳、男性。2015年、食道悪性黒色腫と診断された。BRAF変異陰性。後腹膜、腸間膜に多発転移あり。ニボルマブ投与を開始し、腹膜播種病変は画像上消失した。原発巣と近傍のリンパ節転移は残存したため、食道亜全摘+所属リンパ節郭清術を施行した。術後、ニボルマブ投与を再開したが、脳転移が出現。イピリムマブ投与に変更し、全脳照射を施行したが、永眠された。症例2:72歳、女性。2015年、食道悪性黒色腫と診断された。BRAF変異陰性。PET/CTにてL2椎体に転移あり。ニボルマブ投与により転移巣は画像上消失し、原発巣は色素沈着のみとなった。ニボルマブ投与継続していたが、食道原発巣の再発を認め、食道亜全摘+所属リンパ節郭清術を施行した。術後、無治療で経過観察中であるが、現在のところ再発、転移はない。進行期の食道悪性黒色腫にニボルマブが奏効した2例を経験したので、報告した。(著者抄録)

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  • 抗BP180NC16a抗体陰性で、経過中に全長BP180ELISA法にて診断し得た水疱性類天疱瘡の1例

    石井 芙美, 三宅 智子, 川上 佳夫, 森田 安理, 山崎 修, 泉 健太郎, 西江 渉, 森実 真

    西日本皮膚科   81 ( 3 )   234 - 234   2019.6

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  • 岡山大学病院皮膚科において2013年度から2017年度に実施された金属パッチテスト301症例のまとめ

    高橋 正幸, 横山 恵美, 安原 千夏, 橋本 倫子, 加持 達弥, 三宅 智子, 川上 佳夫, 平井 陽至, 山崎 修, 森実 真

    西日本皮膚科   81 ( 3 )   240 - 240   2019.6

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  • 有茎広背筋皮弁で加療した冠動脈ステント留置後の右肩甲部放射線性皮膚潰瘍の1例

    森田 安理, 山崎 修, 石井 芙美, 川上 佳夫, 加持 達弥, 竹原 彩, 大塚 正樹, 森実 真

    西日本皮膚科   81 ( 3 )   242 - 242   2019.6

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  • ニボルマブが著効した悪性黒色腫in-transit転移における腫瘍浸潤リンパ球の解析

    神野 泰輔, 山崎 修, 眞部 恵子, 三宅 智子, 中川 裕貴, 森実 真, 岩月 啓氏

    西日本皮膚科   81 ( 3 )   251 - 251   2019.6

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  • 陽子線治療後に免疫チェックポイント阻害薬を投与した悪性黒色腫の3例

    加持 達弥, 山崎 修, 森実 真, 松尾 俊彦, 田端 雅弘, 勝井 邦彰, 脇 隆博

    日本皮膚科学会雑誌   129 ( 5 )   1177 - 1177   2019.5

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  • 長期に免疫抑制剤使用中に発症する重篤なヘルペスウイルス関連合併症の予測マーカーの検討

    三宅 智子, 濱田 利久, 平井 陽至, 加持 達弥, 山崎 修, 青山 裕美, 岩月 啓氏, 森実 真

    日本皮膚科学会雑誌   129 ( 5 )   1165 - 1165   2019.5

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  • アベルマブを投与したメルケル細胞癌の2例

    神野 泰輔, 山崎 修, 石井 芙美, 横山 恵美, 川上 佳夫, 森実 真, 野村 隼人

    日本皮膚悪性腫瘍学会学術大会プログラム・抄録集   35回   140 - 140   2019.4

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  • 胃癌を合併した胃転移悪性黒色腫

    立花 宏太, 篠倉 美理, 川上 佳夫, 山崎 修, 森実 真, 丸中 秀格, 榮 浩行, 香川 俊輔, 藤原 俊義, 脇 隆博

    日本皮膚悪性腫瘍学会学術大会プログラム・抄録集   35回   168 - 168   2019.4

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  • 高齢者の治療:あなたならどうする ハンセン病患者に生じた有棘細胞癌

    安富 陽平, 山崎 修, 立花 宏太, 杉本 佐江子, 森実 真

    日本皮膚悪性腫瘍学会学術大会プログラム・抄録集   35回   82 - 82   2019.4

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  • イピリムマブ投与後にACTH単独欠損症を発症した悪性黒色腫の1例

    篠倉 美理, 藤本 裕子, 神野 泰輔, 山崎 修, 森実 真

    日本皮膚科学会雑誌   129 ( 3 )   376 - 376   2019.3

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  • 患者への情報提供を目的とした頭部血管肉腫に対する化学放射線療法の有害事象の検討

    黒田 桂子, 山崎 修, 三宅 智子, 加持 達弥, 岩月 啓氏, 森実 真

    日本皮膚科学会雑誌   129 ( 2 )   215 - 215   2019.2

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  • Dabrafenib/Trametinib併用療法を施行した進行期悪性黒色腫の10例

    藤本 裕子, 加持 達弥, 山崎 修, 森実 真, 松三 友子

    日本皮膚科学会雑誌   128 ( 12 )   2691 - 2691   2018.11

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  • 隆起性皮膚線維肉腫が疑われ、COL1A1-PDGFB融合遺伝子解析を行った5例

    谷本 尚吾, 三宅 智子, 加持 達弥, 森実 真, 山崎 修, 岩月 啓氏, 岩佐 哲志, 金子 栄

    日本皮膚科学会雑誌   128 ( 3 )   463 - 463   2018.3

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  • 当科における皮膚動脈炎の予後不良因子の検討

    加藤 あずさ, 濱田 利久, 山下 珠代, 山本 絢乃, 光井 聖子, 松三 友子, 三宅 智子, 深松 紘子, 平井 陽至, 加持 達弥, 森実 真, 山崎 修, 岩月 啓氏

    日本皮膚科学会雑誌   128 ( 2 )   270 - 270   2018.2

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  • Critical role of novel receptors to S100A8/A9, EMMPRIN and NPTNβ, on keratinocyte growth and inflammation in atopic dermatitis

    49 ( 6 )   594 - 598   2017

  • Critical role of novel receptors to S100A8/A9, EMMPRIN and NPTNβ, on keratinocyte growth and inflammation in atopic dermatitis

    67 ( 6 )   594 - 598   2017

  • 抗PD-1抗体治療後BRAF阻害療法中の進行期メラノーマ患者に生じた原田病 抗PD-1投与前後の免疫応答を含めて

    岩月 啓氏, 山崎 修, 加持 達弥, 濱田 利久, 森実 真, 立川 聖子, 松尾 俊彦, 栄川 伸吾, 鵜殿 平一郎

    西日本皮膚科   78 ( 5 )   554 - 554   2016.10

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  • 抗セントロメア抗体陽性全身性強皮症患者に生じたサルコイドーシス HLA-DR解析と全身性ステロイド療法について

    岩月 啓氏, 平井 陽至, 山崎 修, 濱田 利久, 森実 真, 三宅 智子, 加持 達弥, 梅村 啓史, 武藤 正彦

    日本皮膚科学会雑誌   126 ( 6 )   1152 - 1152   2016.5

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  • 病院現況および患者視点からみた進行期悪性黒色腫に対する新規治療

    山崎 修, 加持 達弥, 平井 陽至, 梅村 啓史, 森実 真, 濱田 利久, 岩月 啓氏, 勝井 邦彰, 合地 明

    日本皮膚科学会雑誌   126 ( 5 )   935 - 935   2016.5

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  • 【皮膚疾患ペディア】角化症 Gibertばら色粃糠疹

    日本医師会雑誌   145 ( S2 )   S213-S213   2016

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  • レンサ球菌感染症のすべて

    山崎修, 森実真

    Monthly Book Derma   242   71 - 76   2016

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  • New Treatment of Advanced Melanoma from the Viewpoint of Patient and Hospital Management

    126 ( 9 )   1709 - 1716   2016

  • 【皮膚疾患ペディア】角化症 乾癬

    日本医師会雑誌   145 ( S2 )   S208-S209   2016

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  • 【皮膚疾患ペディア】角化症 扁平苔癬

    日本医師会雑誌   145 ( S2 )   S211-S212   2016

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  • 処置室の感染対策はどうしていますか?

    Visual Dermatology   13 ( 6 )   706 - 708   2014

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  • 乾癬の治療(生物学的製剤を除く) 乾癬の内服療法 国内外の知見から(解説)

    森実真

    日本皮膚科学会雑誌   123 ( 13 )   2549 - 2551   2013

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  • 皮膚の炎症と表皮バリア機能

    アレルギーの臨床   33 ( 2 )   48 - 52   2013

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  • Th2 cytokines increase kallikrein 7 expression and function in patients with atopic dermatitis

    125 ( 3 )   217 - 220   2013

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  • Hematological complications of epidermolysis bullosa

    66 ( 5 )   50 - 53   2012.4

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  • 後天性魚鱗癬との鑑別を要した魚鱗癬様菌状息肉症

    森実 真

    Visual Dermatology   11 ( 9 )   920 - 921   2012

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  • Epstein-Barrウイルス感染症と種痘様水疱症(共著)

    平井陽至, 山本剛伸, 三宅智子, 山内晶子, 藤井一恭, 森実真, 岩月啓氏

    Monthly Book Derma   191 ( 191 )   48 - 53   2012

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    種痘様水疱症(hydroa vacciniforme;HV)は日光露光部に水疱性または痂皮性小丘疹を繰り返す光線過敏症である。皮疹部組織ではEBウイルス(EBV)が潜伏感染したT細胞が浸潤細胞の数%〜20%を占め、またそれ以上の数の細胞障害性T細胞(CTL)の反応性浸潤を認める。検査上では末梢血EBVゲノム数の増加とγδT細胞増多が認められるが、全身症状やEBV抗体価、血液学的な異常は通常認められない。大多数の例は思春期までに自然治癒する予後良好な疾患であるが、重篤な皮膚症状や発熱、リンパ節腫脹などの全身症状、末梢血EBVゲノム数が著明に増加するような重症型HVは、慢性活動性EBV感染症(CAEBV)や蚊刺過敏症(HMB)に伴って発症することがある。そのような例では自然軽快せず悪性リンパ腫、EBV関連血球貪食症候群などへ進展することが多い。(著者抄録)

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  • EDTAおよび表皮剥脱毒素(ET)処理ELISA法による天疱瘡自己抗体のエピトープ解析

    神谷 浩二, 青山 裕美, 浮田 彩, 久保 亮治, 山崎 修, 白藤 宜紀, 藤井 一恭, 森実 真, 岩月 啓氏

    日本皮膚科学会雑誌   121 ( 3 )   589 - 589   2011.3

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  • 特集 知っておきたい皮膚科の知識 専門医の診方・治し方 乾癬

    森実真

    耳鼻咽喉j科・頭頸部外科   83 ( 12 )   923 - 925   2011

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  • Kallikrein Expression in Keratinocytes and Skin Barrier

    MORIZANE Shin

    Hifu no kagaku   10 ( 16 )   29 - 33   2011

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    Language:Japanese   Publisher:Meeting of Osaka Dermatological Association/Meeting of Keiji Dermatological Association  

    Serine protease tissue kallikreins influence epidermal barrier function. In epidermal keratinocytes, kallikrein 5 (trypsin-like serine protease) and kallikrein 7 (chymotrypsin-like serine protease) are abundantly expressed. These kallikreins are involved in desquamation in normal skin. In atopic dermatitis lesion, tissue kallikreins expression is aberrantly increased, which changes the balanace with the protease inhibitors, leads to the degradation of adhesion molecules and facilitates the entries of microbes and allergen into the epidermis. Our investigation has suggested that Th2 cytokines contribute to epidermal barrier dysfunction by changing the balance between tissue kallikreins and the protease inhibitors.Skin Research, Suppl. 16: 29-33, 2011

    DOI: 10.11340/skinresearch.10.Suppl.16_29

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  • 細菌アレルギーとベーチェット病(共著)

    森実真, 山崎修

    皮膚アレルギーフロンティア   9 ( 2 )   109 - 113   2011

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  • Erythema Nodosum Associated with Crohn's Disease

    53 ( 6 )   817 - 819   2011

  • カポジ水痘様発疹症の複数回罹患

    森実 真

    日本医事新報   ( 4495 )   80 - 81   2010

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  • 多形滲出性紅斑における抗菌蛋白質granulysinの役割

    大野 貴司, 白藤 宜紀, 森実 真, 青地 聖子, 山崎 修, 岩月 啓氏

    西日本皮膚科   69 ( 3 )   327 - 327   2007.6

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  • 表皮衛星細胞壊死形成における細胞障害性抗菌蛋白質グラニュライシンの関与

    大野 貴司, 白藤 宜紀, 森実 真, 青地 聖子, 山崎 修, 岩月 啓氏

    日本研究皮膚科学会年次学術大会・総会プログラム   32回   132 - 132   2007.4

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  • 皮膚細菌感染症におけるPanton-Valentine型ロイコシジンの発現制御機構

    山崎 修, 森実 真, 岩月 啓氏, 加藤 文紀, 金子 淳

    日本皮膚科学会雑誌   117 ( 4 )   721 - 721   2007.3

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  • Panton-Valentine型ロイコシジン(PVL)と皮膚感染症

    山崎 修, 森実 真, 岩月 啓氏, 荒田 次郎, 武藤 正彦

    西日本皮膚科   68 ( 6 )   721 - 721   2006.12

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  • 皮膚における抗菌ペプチドの発現部位と発現細胞の検討

    大野 貴司, 白藤 宜紀, 森 実真, 許 郁江, 山崎 修, 岩月 啓氏

    山口医学   55 ( 5 )   188 - 188   2006.10

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  • 木村病.

    鈴木大介, 辻 和英, 森実 真, 浅越健治, 岩月啓氏

    皮膚病診療   2006

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  • 偽性腸閉塞を伴ったoverlap症候群と全身性強皮症.

    樫野かおり, 松浦浩徳, 中西 元, 辻 和英, 野村知代, 森實 真, 岩月啓氏, 岡田裕之, 松下貴史

    臨床皮膚科   60 ( 3 )   247 - 251   2006

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  • Panton-Valentine型ロイコシジン(PVL)産生黄色ブドウ球菌によるせつの臨床的検討

    山崎 修, 森実 真, 岩月 啓氏, 金子 淳, 神尾 好是, 荒田 次郎

    日本小児皮膚科学会雑誌   24 ( 2 )   192 - 192   2005.11

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  • Aneurysmal fibrous histiocytoma?皮膚超音波所見と病理組織所見の比較検討.

    森實 真, 浅越健治, 松浦浩徳, 大野貴司, 岩月啓氏

    皮膚科の臨床   2005

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  • ステロイド内服療法が奏効した後頭部懸垂性苺状血管腫の1例.

    濱田利久, 浅越健治, 郷原亜以子, 森實 真, 岩月啓氏, 高村志保, 片山治子

    皮膚科の臨床   47 ( 8 )   1119 - 1122   2005

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    9ヵ月女児.生後2週間後から後頭部に爪甲大の紅色局面が出現し,近医にてレーザー治療,ステロイド外用を受けていたが紅斑は増大,腫瘤を形成し,出血を伴うようになった.後頭部右側から後頸部にかけて広基性に隆起する腫瘤を有し,腫瘤頂部からは物理的刺激により容易に出血した.Factor VIII,CD34,glucose transporter protein 1(GLUT1)は,いずれも増生する血管内皮細胞に染色された.血液検査上,出血傾向はなく,増大傾向もなくなっていたが,頻回に出血を繰返すため,治療としてステロイドの内服を選択した.その結果,腫瘤の色調の退色,容積の減少がみられ,漸減中も腫瘤の再増大はみられず,投与開始から6ヵ月後にステロイドを終了した.ステロイド内服は,本症例のように腫瘤を形成し,易出血性を伴うような場合には,早期でなくても試みられるべき治療法と考えられた

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  • 低pH環境下における黄色ブドウ球菌定着について

    秋山 尚範, 森実 真, 山崎 修, 大野 貴司, 岩月 啓氏

    西日本皮膚科   66 ( 5 )   528 - 528   2004.10

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  • 下顎部病変治療後に両鼠径部巨大腫瘤を認めた木村病

    鈴木 大介, 辻 和英, 森実 真, 山崎 修, 牧野 英一, 浅越 健治, 岩月 啓氏

    西日本皮膚科   66 ( 3 )   302 - 302   2004.6

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  • 共焦点レーザー顕微鏡を用いたレンサ球菌性膿痂疹の研究

    秋山 尚範, 森実 真, 山崎 修, 大野 貴司, 岩月 啓氏

    西日本皮膚科   66 ( 3 )   313 - 313   2004.6

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  • 手指伸展障害をきたしたPanton-Valentine型ロイコシジン産生黄色ブドウ球菌による皮下膿瘍

    森実 真, 大藤 玲子, 山崎 修, 秋山 尚範, 岩月 啓氏

    日本皮膚科学会雑誌   114 ( 7 )   1298 - 1298   2004.6

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  • 魚鱗癬様菌状息肉症の3例

    森實 真, 内藤 洋子, 山崎 修, 辻 和英, 浅越 健治, 岩月 啓氏

    西日本皮膚科   66 ( 3 )   301 - 301   2004.6

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  • 深在性膿皮症における黄色ブドウ球菌が産生するPanton-Valentine型ロイコシジンの病因的意義

    森實 真, 山崎 修, 秋山 尚範, 岩月 啓氏, 金子 淳, 神尾 好是

    日本研究皮膚科学会年次学術大会・総会プログラム   29回   116 - 116   2004.4

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  • マウス皮膚炎部黄色ブドウ球菌感染症に対する低pH外用薬の効果

    秋山 尚範, 森実 真, 山崎 修, 大野 貴司, 岩月 啓氏, 市川 秀之

    日本皮膚科学会雑誌   114 ( 3 )   619 - 619   2004.3

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  • 皮膚細菌感染症病変におけるリンパ球産生抗菌蛋白質granulysinの局在

    大野 貴司, 許 郁江, 山崎 修, 森実 真, 秋山 尚範, 岩月 啓氏

    日本皮膚科学会雑誌   113 ( 5 )   762 - 762   2003.4

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  • Panton-Valentine型ロイコシジン産生黄色ブドウ球菌の臨床的・細菌学的検討

    山崎 修, 森実 真, 秋山 尚範, 岩月 啓氏, 金子 淳, 神尾 好是, 荒田 次郎

    日本皮膚科学会雑誌   113 ( 5 )   786 - 786   2003.4

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  • 平滑筋母斑の1例.

    森實 真, 山田 潤, 山本剛伸, 大野貴司, 岩月啓氏

    皮膚科の臨床   2003

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  • 塩酸ミノサイクリンによる即時型アレルギーの2例 反応閾値についての検討

    中安 慎二, 森実 真, 井上 雅子, 山崎 修, 荒田 次郎

    皮膚科の臨床   44 ( 6 )   639 - 641   2002.6

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    49歳女(症例1).4年前に感冒症状で塩酸ミノサイクリン点滴を受けたところ,開始直後に呼吸困難,意識レベル低下を来たした.薬剤アレルギーの精査で,濃度1mg/mlのスクラッチテストを左前腕内側に施行した.15分後に同部位に15×27mmの紅斑が出現し,30分後には6×7mmの膨疹となった.更に45分後,腰仙部に手掌大の膨疹が出現した.膨疹は数時間で消失し,呼吸困難,意識レベル消失はなかった.61歳男(症例2).ミノマイシン内服で30〜40分後,全身に紅斑が出現し,数時間後に消失した.塩酸ミノサイクリン1mg/mlの皮内テストを施行したところ,15分後に同部位に15×10mmの膨疹を伴う35×40mmの紅斑が出現し,30分後には30×35mmに縮小した.濃度0.05mg/mlでは15分後に8×7mmの膨疹を伴う15×20mmの紅斑が出現した

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  • 塩酸ミノサイクリンによる即時型アレルギーの2例

    中安 慎二, 森実 真, 筒井 雅子, 山崎 修, 荒田 次郎

    日本皮膚科学会雑誌   111 ( 11 )   1603 - 1603   2001.10

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Presentations

  • 炎症性サイトカインと表皮バリア機能異常 Invited

    森実 真

    第53回日本皮膚免疫アレルギー学会学術大会  2023.12.8 

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    Event date: 2023.12.8 - 2023.12.10

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

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  • 当科におけるアトピー性皮膚炎診療・研究2023 Invited

    森実 真

    日本皮膚科学会山形地方会 第404回例会ランチョンセミナー  2023.11.26 

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    Event date: 2023.11.26

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • AD患者さんに当たり前の日常を~思春期ADの病態と治療~ Invited

    森実 真

    第74回日本皮膚科学会中部支部学術大会 ランチョンセミナー8  2023.10.29 

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    Event date: 2023.10.28 - 2023.10.29

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 表皮セリンプロテアーゼ活性の重要性~アトピー性皮膚炎とネザートン症候群の病態を踏まえて~ Invited

    森実 真

    第153回日本皮膚科学会広島地方会 ~田中暁生教授就任記念~  2023.9.2 

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    Event date: 2023.9.2 - 2023.9.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 併存症と乾癬~当科の研究を踏まえて~ Invited

    森実 真

    第38回日本乾癬学会学術大会 モーニングセミナー3  2023.8.26 

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    Event date: 2023.8.25 - 2023.8.26

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • ネザートン症候群の病態と治療up-to-date Invited

    森実 真

    日本皮膚科学会新潟地方会 第410回例会プログラム 講習会2  2023.6.24 

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    Event date: 2023.6.24

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • LL型ハンセン病新規発症例の治療経過

    篠倉 美理, 尾崎 元昭, 川上 佳夫, 梅川 康弘, 吉原 由樹, 山崎 修, 森 実真

    日本ハンセン病学会雑誌  2021.5  日本ハンセン病学会

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    Event date: 2021.5

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  • 好中球関連皮膚疾患 Up to date 好中球関連皮膚疾患の共通のメカニズムは?

    森実 真

    日本皮膚科学会雑誌  2021.5  (公社)日本皮膚科学会

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    Event date: 2021.5

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  • ここまでわかったアトピー性皮膚炎における表皮バリア機能異常

    森実 真

    日本皮膚科学会雑誌  2021.5  (公社)日本皮膚科学会

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    Event date: 2021.5

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  • 免疫チェックポイント阻害薬でCRとなり,中止した悪性黒色腫6例

    杉原 悟, 山崎 修, 森実 真, 加持 達弥, 南 満芳

    日本皮膚科学会雑誌  2021.5  (公社)日本皮膚科学会

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    Event date: 2021.5

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  • Lanosterol synthase(LSS)遺伝子の複合ヘテロ接合型変異によって生じた先天性乏毛症の2例

    村田 真美, 林 良太, 川上 佳夫, 森実 真, 下村 裕

    日本皮膚科学会雑誌  2021.5  (公社)日本皮膚科学会

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    Event date: 2021.5

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  • DFSPとの鑑別と整容面への配慮を要した顔面のdeep penetrating dermatofibroma(DPDF)の3例

    谷本 尚吾, 三宅 智子, 杉原 悟, 山崎 修, 森実 真, 加持 達弥, 谷口 恒平

    日本皮膚科学会雑誌  2021.5  (公社)日本皮膚科学会

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    Event date: 2021.5

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  • モガムリズマブ投与後に移植片対宿主病が重症化した成人T細胞白血病の3例

    横山 恵美, 赤松 由規, 山崎 修, 山本 晃, 大山 矩史, 神原 由依, 淺田 騰, 谷口 恒平, 吉野 正, 森実 真

    日本皮膚科学会雑誌  2021.5  (公社)日本皮膚科学会

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    Event date: 2021.5

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  • 無疹部の皮膚生検により診断された遺伝性ATTR型アミロイドーシス

    小橋 美那, 平井 陽至, 篠倉 美理, 横山 恵美, 加持 達弥, 山崎 修, 森実 真, 中村 一文, 山下 徹, 山下 太郎

    日本皮膚科学会雑誌  2021.2  (公社)日本皮膚科学会

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    Event date: 2021.2

    Language:Japanese  

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  • 二次感染を契機に増悪し、モガムリズマブが奏効したセザリー症候群の1例

    赤松 由規, 平井 陽至, 渡部 桃子, 中川 裕貴, 小橋 美那, 内藤 聖子, 三宅 智子, 山崎 修, 森実 真, 山本 晃, 淺田 騰

    日本皮膚科学会雑誌  2021.2  (公社)日本皮膚科学会

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    Event date: 2021.2

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  • GJB2遺伝子のヘテロ接合性変異を同定したVohwinkel症候群の1例

    池田 賢太, 中川 裕貴, 内藤 聖子, 川上 佳夫, 山崎 修, 森実 真, 伊藤 靖敏, 武市 拓也, 秋山 真志

    日本皮膚科学会雑誌  2021.2  (公社)日本皮膚科学会

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    Event date: 2021.2

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  • 1次治療としてタキサン系化学療法を施行した進行期乳房外パジェット病の8例

    山崎 江利子, 山崎 修, 杉原 悟, 三宅 智子, 森実 真, 加持 達弥

    日本皮膚科学会雑誌  2021.2  (公社)日本皮膚科学会

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    Event date: 2021.2

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  • 岡山大学における乾癬診療・研究 Invited

    森実 真

    日本皮膚科学会愛媛地方会第69回学術大会 

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    Event date: 2019.3.30

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 乾癬診療と基礎研究 Invited

    森実 真

    第3回中国五県乾癬治療を語る会 

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    Event date: 2019.3.23

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 種痘様水疱症と蚊刺過敏症における血球・血漿EBV DNA定量の臨床的意義

    三宅智子、山本剛伸、平井陽至、岩月啓氏、森実 真

    第28回EBウイルス感染症研究会 

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    Event date: 2019.3.17

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 当科における乾癬診療・研究?外用剤から生物学的製剤まで? Invited

    森実 真

    日本皮膚科学会第226回熊本地方会 

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    Event date: 2019.3.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 皮膚科が関わる指定難病

    森実 真

    第2回岡山県難病診療連携拠点病院研修会 

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    Event date: 2019.2.25

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 経皮感作とスキンケア Invited

    森実 真

    岡山県医師会学術奨励賞受賞講演並びに日本医師会生涯教育講座 

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    Event date: 2019.2.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 当科における乾癬診療・研究 Invited

    森実 真

    第21回岐阜臨床皮膚科懇話会 

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    Event date: 2019.1.26

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • Exfoliative cheilitis

    濱田利久,芦田日美野,細川洋一郎,池田政身,森実 真

    第276回 日本皮膚科学会岡山地方会 

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    Event date: 2019.1.19

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 親族に同症のあるnasal dermoid sinus cystの1例

    西垣貴美子,山? 修,篠倉美理,川上佳夫,三宅智子,森実 真,香曽我部純子

    第276回 日本皮膚科学会岡山地方会 

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    Event date: 2019.1.19

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 臭化カリウム減量により症状が軽快した臭素疹の1例

    安富陽平,川上佳夫,平井陽至,山? 修,森実 真

    第276回 日本皮膚科学会岡山地方会 

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    Event date: 2019.1.19

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • イピリムマブ投与後にACTH単独欠損症を発症した悪性黒色腫の1例

    篠倉美理,藤本裕子,神野泰輔,山? 修,森実 真

    第276回 日本皮膚科学会岡山地方会 

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    Event date: 2019.1.19

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 抗PD-1抗体投与後に発症した類天疱瘡の2例

    横山恵美,森田安理,山? 修,森実 真,久保寿男,高本 篤

    第276回 日本皮膚科学会岡山地方会 

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    Event date: 2019.1.19

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 結節性硬化症の皮膚病変に対しシロリムス局所外用療法を導入した4例

    小橋美那,平井陽至,三宅智子,加持達弥,川上佳夫,山? 修,森実 真

    第276回 日本皮膚科学会岡山地方会 

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    Event date: 2019.1.19

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  • 岡山大学における乾癬診療・研究 Invited

    森実 真

    鳥取県臨床皮膚科医会学術講演会 

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    Event date: 2019.1.17

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 乾癬病変部角化細胞におけるTNF-α/IL-17A/IFN-γの相乗作用 Invited

    森実 真

    乾癬治療?エキスパートから学ぶ基礎研究? 

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    Event date: 2019.1.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • ハンセン病LL型新規発症の1例

    篠倉美理、杉本佐江子、川上佳夫、山? 修、吉原由樹、多田讓治、尾崎元昭、石井則久、森実 真

    第82回日本皮膚科学会東京支部学術大会 

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    Event date: 2018.12.1 - 2018.12.2

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 当科におけるIL-17Aを標的とした乾癬診療・研究 Invited

    森実 真

    ふじのくに乾癬Expert Meeting 

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    Event date: 2018.11.29

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • IL-17Aを標的とした乾癬治療・研究 Invited

    森実 真

    第2回西讃地区トルツ学術講演会 

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    Event date: 2018.11.27

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 当科における皮膚炎診療 Invited

    森実 真

    和気医師会学術講演会 

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    Event date: 2018.11.22

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 円盤状エリテマトーデスに対するヒドロキシクロロキンの使用経験

    安富陽平、三宅智子、橋本倫子、山? 修、森実 真

    第45回岡山膠原病研究会 

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    Event date: 2018.11.20

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • メシル酸ガレノキサシン水和物の(ジェニナック)による固定薬疹の一例。

    石井芙美、川上佳夫、三宅智子、森実 真

    第48回日本皮膚免疫アレルギー学会総会学術大会 

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    Event date: 2018.11.16 - 2018.11.18

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • IL-17Aを標的とした乾癬診療・研究 Invited

    森実 真

    日常診療における乾癬治療を考える会 

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    Event date: 2018.11.14

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 乾癬病変部角化細胞におけるTNF-α/IL-17A/IFN-γの相乗作用 Invited

    森実 真

    第70回日本皮膚科学会西部支部学術大会 

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    Event date: 2018.11.10 - 2018.11.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 岡山大学病院皮膚科において2013年度から2017年度に実施された金属パッチテスト301省令のまとめ

    高橋正幸、横山恵美、安原千夏、橋本倫子、加持達弥、三宅智子、川上佳夫、平井陽至、山? 修、森実 真

    第70回日本皮膚科学会西部支部学術大会 

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    Event date: 2018.11.10 - 2018.11.11

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 抗BP180NC16a抗体陰性で、経過中に全長BP180ELISA法にて診断し得た水疱性類天疱瘡の1例

    石井芙美、三宅智子、川上佳夫、森田安理、山? 修、泉 健太郎、西江 渉、森実 真

    第70回日本皮膚科学会西部支部学術大会 

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    Event date: 2018.11.10 - 2018.11.11

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 有茎広背筋皮弁加療した冠動脈ステント留置後の右肩甲部放射性皮膚潰瘍の1例

    森田安理、山? 修、石井芙美、川上佳夫、加持達弥、竹原 彩、大塚正樹、森実 真

    第70回日本皮膚科学会西部支部学術大会 

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    Event date: 2018.11.10 - 2018.11.11

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • ニボルマブが著効した悪性黒色腫in-transit転移における腫瘍浸潤リンパ球の解析

    神野泰輔、山?修、眞部恵子、三宅智子、中川裕貴、森実 真、岩月啓氏

    第70回日本皮膚科学会西部支部学術大会 

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    Event date: 2018.11.10 - 2018.11.11

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • ソメイヨシノによるアレルギーと抗原蛋白質の解析

    光井聖子、白築理恵、神谷浩二、千貫祐子、森田栄伸、森実 真

    第70回日本皮膚科学会西部支部学術大会 

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    Event date: 2018.11.10 - 2018.11.11

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 乾癬と糖尿病 Invited

    森実 真

    Dermatology Diabetes Forum 

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    Event date: 2018.11.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • アトピー性皮膚炎患者における遺伝子多型および角層中セリンプロテアーゼ活性について

    野村隼人、森実 真

    第14回中国研究皮膚科セミナー 

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    Event date: 2018.11.3

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Cetuximab投与中に生じたleukocytoclastic vasculitis

    安富陽平、篠倉美理、神野泰輔、藤本裕子、川上佳夫、山? 修、森実 真

    第82回日本皮膚科学会東部支部学術大会 

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    Event date: 2018.10.6 - 2018.10.7

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Hydroa vacciniforme and hydroa vaccinifotme-like lymphoproliferative disorder: Disease spectrum, responsible T-cell subsets and prognosis International conference

    Iwatsuki K, Miyake T, Hirai Y, Morizane S

    EORTC-CLTF 2018 meeting on cutaneous lymphoma: biology, treatment, and patient view 

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    Event date: 2018.9.27 - 2018.9.29

    Language:English   Presentation type:Oral presentation (general)  

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  • Indolent hematodermic CD4+ T-cell lymphoma lacking surface CD3 expression: Structural and functional deficiency of T-cell receptor/CD3 complex International conference

    Iwatsuki K, Nakagawa Y, Hamada T, Uchida T, Morizane S, Takahashi T, Watanabe T, Miyake T, Manabe K, Asagoe K

    EORTC-CLTF 2018 meeting on cutaneous lymphoma: biology, treatment, and patient view 

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    Event date: 2018.9.27 - 2018.9.29

    Language:English   Presentation type:Oral presentation (general)  

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  • 長期残存した、草刈り機の破片による左下腿の皮下金属異物

    立花宏太、山? 修、篠倉美理、神野泰輔、藤本裕子、森実 真、梅村啓史

    第275回日本皮膚科学会岡山地方会 森実 真 教授就任記念地方会 

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    Event date: 2018.9.9

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • ステロイド内服が奏効した関節運動障害合併皮下型サルコイドーシスの1例

    篠倉美理、三宅智子、加持達弥、山? 修、森実 真、安井陽子

    第275回日本皮膚科学会岡山地方会 森実 真 教授就任記念地方会 

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    Event date: 2018.9.9

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  • Dabrafenib/Trametinib 併用療法を施行した進行期悪性黒色腫の10例

    藤本裕子、加持達弥、山? 修、森実 真、松三友子

    第275回日本皮膚科学会岡山地方会 森実 真 教授就任記念地方会 

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    Event date: 2018.9.9

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 当科における炎症性皮膚疾患診療?乾癬を中心に? Invited

    森実 真

    佐世保皮膚科医会学術講演会 長崎県医師会生涯教育認定講座 

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    Event date: 2018.6.13

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 口腔粘膜部に生じたsolitary fibrous tumorの1例

    保利純子、光井聖子、川上佳夫、三宅智子、森実 真、岩月啓氏、小田和歌子

    第274回日本皮膚科学会岡山地方会 

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    Event date: 2018.5.12

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 乾癬とサイトカインに関する最近の話題

    森実 真

    IL-17A WEB Seminar 

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    Event date: 2018.4.25

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 乾癬の診断・治療と私が考える皮膚科医の魅力 Invited

    森実 真

    EXPERTと考える若手乾癬研究会~winter~  2023.12.23 

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 好中球関連皮膚疾患とスペビゴへの期待 Invited

    森実 真

    膿疱性乾癬 WEBセミナー  2023.11.30 

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 帯状疱疹予防 UPDATE 2023 Invited

    森実 真

    シングリックス追加承認(接種対象者拡大)WEB講演会  2023.11.29 

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  • 当科における乾癬診療up-to-date2023 Invited

    森実 真

    美作学術講演会  2023.11.20 

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  • 当科における乾癬診療・研究とグセルクマブ Invited

    森実 真

    第16回北海道皮膚免疫セミナー  2023.10.11 

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  • アトピー性皮膚炎におけるJAK-STATシグナルの重要性と最新の治療 Invited

    森実 真

    Current Advanced Therapy Seminar  2023.9.27 

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  • アトピー性皮膚炎診療・研究2023 Invited

    森実 真

    Dermatology Clinical Seminar  2023.9.22 

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  • アトピー性皮膚炎と移行期医療 Invited

    森実 真

    第24回山陽小児アレルギー研究会  2023.9.14 

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  • アトピー性皮膚炎診療 up-to-date 2023~モイゼルト軟膏の使用について考える~ Invited

    森実 真

    モイゼルト軟膏発売1周年記念講演会  2023.9.12 

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • 表皮バリア機能異常とJAK-STATシグナル~サイバインコの作用機序とEBM~ Invited

    森実 真

    サイバインコ アトピー性皮膚炎インターネットシンポジウム  2023.8.23 

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  • 乾癬診療 Up-to-date ~乾癬治療におけるグセルクマブの位置づけ~ Invited

    森実 真

    乾癬 Update Seminar  2023.8.18 

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  • 当科におけるAD診療・研究と最新の治療 Invited

    森実 真

    第26回北陸皮膚科セミナー  2023.8.6 

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  • 皮膚バリア機能異常を考慮したアトピー性皮膚炎診療 Invited

    森実 真

    宇和島医師会 学術講演会  2023.8.4 

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  • 皮膚科学分野における医用工学update Invited

    森実 真

    第130回岡山県医用工学研究会・オンラインセミナー 「感覚器領域の医用工学アップデート」  2023.7.28 

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  • IL-17A/IL-17Fを阻害する意義と ビンゼレックスの位置付け Invited

    森実 真

    ビンゼレックス発売1周年記念講演会 in 中国  2023.7.22 

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • アトピー性皮膚炎における JAK-STATシグナルの重要性と最新の治療 Invited

    森実 真

    第410回日本皮膚科学会新潟地方会 共催セミナー  2023.6.24 

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  • 当科における乾癬診療・研究とビンゼレックスの位置づけ Invited

    森実 真

    日本皮膚科学会愛媛地方会第76回学術大会 イブニングセミナー  2023.3.25 

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  • 岡山大学病院におけるPsA診療 Invited

    森実 真

    SAGA Psoriasis Cooperation Seminar  2023.3.17 

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  • アトピー性皮膚炎における JAK-STATシグナルの重要性と最新の治療 Invited

    森実 真

    Atopic Dermatitis Seminar  2023.3.16 

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  • アトピー性皮膚炎診療・研究2023 Invited

    森実 真

    サイバインコ発売一周年記念講演会 from 岡山  2023.3.3 

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  • AD病変部角化細胞からみた JAK-STATシグナルの重要性 Invited

    森実 真

    サイバインコ発売一周年記念講演会from長崎  2023.2.22 

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  • 皮膚バリア機能異常を考慮したアトピー性皮膚炎診療 Invited

    森実 真

    第389回沖縄皮膚科勉強会  2023.2.16 

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  • 岡山大学のアトピー性皮膚炎治療 ~ミチーガの使用経験も含めて~ Invited

    森実 真

    第二回西播磨皮膚疾患懇話会  2023.2.8 

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  • 全身性炎症性疾患としての乾癬~皮膚科医の立場から~ Invited

    森実 真

    かかりつけ医のための生活習慣病に関する自己免疫性疾患セミナー~乾癬・乾癬性関節炎の病態と治療~  2022.9.28 

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  • アトピー性皮膚炎の病態理解と最新の治療 Invited

    森実 真

    ワカモン会 in KAGAWA  2022.9.17 

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  • ミチーガを使用する際のポイント Invited

    森実 真

    ミチーガ発売記念講演会in岡山  2022.9.16 

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  • アトピー性皮膚炎患者における表皮バリア機能異常 Invited

    森実 真

    Atopic dermatitis WEB Meeting II  2022.9.13 

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  • リサンキズマブで加療した乾癬性関節炎の3例

    廣瀬 梓, 吉永泰彦, 西山 進, 相田哲史, 川上佳夫, 森実 真

    第37回日本乾癬学会学術大会  2022.9.9 

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  • セクキヌマブが奏功した尋常性乾癬の小児例

    松田吉弘, 川上佳夫, 森実 真, 八代将登

    第37回日本乾癬学会学術大会  2022.9.9 

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  • 減量・代謝改善手術後に軽快した尋常性乾癬の1例

    高須賀琴巳, 横山恵美, 川上佳夫, 和田 淳, 香川俊輔, 森実 真

    第37回日本乾癬学会学術大会  2022.9.9 

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  • 脱毛から診断に至った高齢期発症の全身性エリテマトーデスの1例

    岡野真理, 横山恵美, 川上佳夫, 勝山恵理, 山口春佳, 森実 真

    第287回日本皮膚科学会岡山地方会  2022.9.4 

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  • BNT162b2 mRNA COVID-19ワクチン接種後に発症したacute diffuse and total alopecia of the female scalpの1例

    川本雅也, 川上佳夫, 松田吉弘, 平井陽至, 多田譲治, 森実 真

    第287回日本皮膚科学会岡山地方会  2022.9.4 

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  • 粉瘤を発生母地とした臀部有棘細胞癌の1例

    蓮井謙一, 立花宏太, 渡辺敏之, 寺石文則, 中井友美, 川上佳夫, 野村隼人, 森実 真

    第287回日本皮膚科学会岡山地方会  2022.9.4 

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  • 臀部皮下腫瘤を主訴に受診し痔瘻と診断した2例

    蓮井謙一, 立花宏太, 杉原 悟, 川上佳夫, 森実 真

    第37回日本皮膚外科学会総会・学術集会  2022.9.3 

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  • 新規ALOX12B遺伝子病的バリアントを認めた先天性魚鱗癬様紅皮症の1例

    前 琴絵, 梶田 藍, 野田達宏, 武市拓也, 川上佳夫, 秋山真志, 森実 真

    第86回日本皮膚科学会東部支部学術大会  2022.8.27 

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  • 逆行性顔面動脈皮弁を用いて再建した口唇有棘細胞癌の1例

    杉原 悟, 山﨑 修, 立花宏太, 森実 真

    第86回日本皮膚科学会東部支部学術大会  2022.8.27 

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  • 休止期脱毛と爪甲剥離を呈したCronkhite-Canada症候群の1例

    横溝紗佑里, 野村隼人, 中川裕貴, 森実 真, 衣笠秀明

    第86回日本皮膚科学会東部支部学術大会  2022.8.27 

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  • 乾癬治療とIL-23阻害の意義~走る距離に合ったシューズのすゝめ~ Invited

    森実 真

    第86回日本皮膚科学会東部支部学術大会モーニングセミナー  2022.8.27 

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  • 酒さ様皮膚炎の治療中に生じたMorbihan病と考えられた一例

    横溝紗佑里, 横山恵美, 川上佳夫, 森実 真, 安井陽子

    日本皮膚科学会第37回島根地方会-山﨑 修教授就任記念-  2022.8.6 

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  • AD病変部角化細胞からみたJAK-STATシグナルの重要性 Invited

    森実 真

    サイバインコ アトピー性皮膚炎発売記念web講演会in奈良  2022.8.4 

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  • ここまでわかったアトピー性皮膚炎における表皮バリア機能異常 Invited

    森実 真

    第20回皮膚科EBMフォーラム  2022.7.30 

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  • 小児乾癬の診断と治療 Invited

    森実 真

    第4回こどもお肌セミナーin岡山  2022.7.27 

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  • AD病変部角化細胞からみたJAK-STATシグナルの重要性 Invited

    森実 真

    Pfizer Dermatology Seminar  2022.7.8 

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  • アトピー性皮膚炎の最新治療 Invited

    森実 真

    御津医師会学術講演会  2022.7.6 

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  • 血栓性静脈炎および咽頭潰瘍を生じた神経ベーチェット病の1例

    横山恵美, 川上佳夫, 勝山隆行, 丸中秀格, 森実 真

    第49回岡山膠原病研究会  2022.6.28 

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  • AD病変部角化細胞からみたJAK-STATシグナルの重要性 Invited

    森実 真

    サイバインコ発売記念講演会in佐賀  2022.6.17 

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  • AD病変部角化細胞からみたJAK-STATシグナルの重要性 Invited

    森実 真

    山梨県皮膚科医会学術講演会  2022.6.15 

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  • 高脂血症・肥満が乾癬モデルマウスに及ぼす影響の検討

    池田賢太, 向井知之, 赤木貴彦, 浅野澄恵, 金藤秀明, 守田吉孝, 森実 真

    第121回日本皮膚科学会総会  2022.6.2 

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  • サブスペシャリティ専門医の位置づけ Invited

    森実 真

    第121回日本皮膚科学会総会  2022.6.2 

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  • 悪性黒色腫におけるBRAFV600E変異の均一性の免疫組織学的・分子遺伝学的検討

    立花宏太, 杉原 悟, 山﨑 修, 爲政大幾, 後藤啓介, 本間圭一郎, 久木田洋児, 谷口恒平, 森実 真

    第121回日本皮膚科学会総会  2022.6.2 

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  • COVID-19ワクチンの接種後に全身性膿疱性乾癬を発症した2例

    徳田真優, 立花宏太, 川上佳夫, 佐藤志帆, 三宅智子, 内田隆文, 福島智恵, 杉浦一充, 森実 真

    第121回日本皮膚科学会総会  2022.6.2 

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  • AD病変部角化細胞からみたJAK-STATシグナルの重要性 Invited

    森実 真

    サイバインコ発売記念講演会in金沢  2022.5.27 

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  • "結節性多発性動脈炎に伴う難治性下腿潰瘍の原因としてステロイドによる創傷治癒遅延が考えられた1例 "

    浦上仁志, 杉原 悟, 篠倉美理, 森田安理, 竹崎大輝, 森実 真, 光井聖子, 大橋敬司

    第286回日本皮膚科学会岡山地方会  2022.5.14 

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  • 四肢と左臀部に皮下硬結を形成した Extensive Subcutaneous Sarcoidosisの1例

    臼井真菜, 杉原 悟, 立花宏太, 川上佳夫, 森実 真, 中本健太, 大塚文男

    第286回日本皮膚科学会岡山地方会  2022.5.14 

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  • ブロダルマブの自己中断後に尋常性乾癬の膿疱化を生じた乾癬性関節炎の1例

    廣瀬 梓, 吉永泰彦, 三宅智子, 川上佳夫, 森実 真

    第286回日本皮膚科学会岡山地方会  2022.5.14 

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  • 左膝に生じた限局型皮膚ノカルジア症

    澤井希望, 立花宏太, 川上佳夫, 眞部恵子, 芦田日美野, 森実 真

    第286回日本皮膚科学会岡山地方会  2022.5.14 

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  • 乾癬の診療~IL-23の最新の話題を含めて~ Invited

    森実 真

    第58回遠州皮膚科医会  2022.5.11 

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  • アトピー性皮膚炎、乾癬治療について Invited

    森実 真

    薬剤師のための皮膚疾患セミナー  2022.4.27 

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  • ADにおける皮膚バリア機能修復を主眼とした治療戦略 Invited

    森実 真

    Meet The Specialist  2022.4.9 

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  • 当科で経験した医原性免疫不全関連リンパ増殖性疾患10例の検討

    森実 真, 川上佳夫, 竹﨑大輝

    第78回日本皮膚科学会高知地方会~佐野栄紀教授退任記念~  2022.4.2 

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  • 同一病変内に有棘細胞癌と悪性黒色腫が合併した2例

    山﨑 修, 千貫祐子, 古村尚士, 太田征孝, 荒木亜寿香, 山下珠代, 森実 真

    第78回日本皮膚科学会高知地方会~佐野栄紀教授退任記念~  2022.4.2 

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  • アトピー性皮膚炎におけるリンヴォック錠の使いどころ Invited

    森実 真

    アトピー性皮膚炎適応追加記念講演会in姫路  2022.3.16 

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  • ペムブロリズマブ投与後IFNβ局注、イミキモド外用を併用した悪性黒色腫in-transit転移の1例

    山﨑 修, 杉原 悟, 立花宏太, 三宅智子, 森実 真, 柳井広之, 荒川謙三

    日本皮膚科学会第234回熊本地方会福島聡教授就任記念地方会  2022.3.13 

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  • アトピー性皮膚炎治療の変遷と今後の展望 Invited

    森実 真

    AD NEW Guidelines Seminar  2022.2.19 

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  • 表皮角化細胞におけJAK-STAシグナル Invited

    森実 真

    コレクチム軟膏1周年記念講演会  2022.1.21 

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  • 口器除去後に残存したセメント物質により炎症が遷延したマダニ咬傷の1例

    佐藤志帆, 川上佳夫, 安富陽平, 横山恵美, 山﨑 修, 森実 真, 吉岡敏子

    第285回日本皮膚科学会岡山地方会  2022.1.15 

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  • "ラムシルマブ投与中に生じた多発性毛細血管拡張性肉芽腫の1例 "

    臼井真菜, 横山恵美, 野村隼人, 山﨑 修, 森実 真, 市原英基

    第285回日本皮膚科学会岡山地方会  2022.1.15 

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  • 中等症劣性栄養障害型表皮水疱症に対して自家培養表皮移植を施行した1例

    竹﨑大輝, 三宅智子, 瀧川充希子, 野村隼人, 藤原 暖, 夏賀 健, 増地 裕, 山﨑 修, 森実 真

    第285回日本皮膚科学会岡山地方会  2022.1.15 

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  • Gly m 4特異的IgEとプリックテストが陽性であった花粉-食物アレルギー症候群の1例

    森田安理, 野村隼人, 山﨑 修, 森実 真, 髙橋祥子

    第285回日本皮膚科学会岡山地方会  2022.1.15 

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  • 骨折が判明した皮下深部解離性血腫の1例

    安富陽平, 水田康生, 芦田日美野, 三宅智子, 山﨑 修, 森実 真

    第285回日本皮膚科学会岡山地方会  2022.1.15 

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  • 鼡径リンパ節郭清後に発症したanesthesia mumpsの1例

    廣瀬 梓, 臼井真菜, 篠倉美理, 森田安理, 杉原 悟, 山﨑 修, 森実 真, 片岡祐子, 松﨑 孝

    第285回日本皮膚科学会岡山地方会  2022.1.15 

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  • 乳癌治療後に生じた左胸部放射線誘発性血管肉腫

    徳田真優, 杉原 悟, 佐藤志帆, 山口春佳, 立花宏太, 山﨑 修, 森実 真, 高橋正幸

    第285回日本皮膚科学会岡山地方会  2022.1.15 

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  • 生体肝移植後の腹部皮膚血管肉腫の1例

    森田安理, 山﨑 修, 篠倉美理, 廣瀬 梓, 臼井真菜, 森実 真

    日本皮膚科学会第69回香川地方会  2021.12.18 

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  • アトピー性皮膚炎の最新の治療 Invited

    森実 真

    Lilly AD web conference  2021.12.10 

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  • 乾癬診療・研究におけるIL-23の最新の話題 Invited

    森実 真

    SKY-RISE Internet Live Seminar  2021.12.8 

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  • 抗PD-1抗体投与初期に扁平苔癬を生じ、その後2年を経て水疱性類天疱瘡を発症した進行期胃癌の1例

    池田賢太, 横山恵美, 山﨑 修, 香川俊輔, 古賀浩嗣, 石井文人, 森実 真

    岡山県医師会皮膚科部会講演会「岡山県水疱症研究会」  2021.12.8 

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  • アトピー性皮膚炎治療の変遷と今後の展望 Invited

    森実 真

    Atopic Dermatitis biological Seminar  2021.11.24 

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  • トシリズマブ投与後に皮疹を認めたリウマトイド血管炎の2例

    臼井真菜, 横山恵美, 山﨑 修, 森実 真, 勝山隆行, 香曾我部幸, 白藤宜紀

    第72回日本皮膚科学会中部支部学術大会  2021.11.20 

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  • 金属異物によって生じた前頸部pseudolmphoma

    德田真優, 平井陽至, 川上佳夫, 立花宏太, 中川裕貴, 杉原 悟, 三宅智子, 山﨑 修, 森実 真, 眞部恵子, 浅越健治

    第72回日本皮膚科学会中部支部学術大会  2021.11.20 

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  • 点状掌蹠角化症の1例

    池田賢太, 森実 真, 伊藤靖敏, 武市拓也, 秋山真志

    第72回日本皮膚科学会中部支部学術大会  2021.11.20 

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  • 基礎研究の成果から考えるアトピー性皮膚炎診療 Invited

    森実 真

    多摩川AD治療研究会  2021.11.19 

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  • アトピー性皮膚炎治療におけるJAK阻害薬の役割 Invited

    森実 真

    リンヴォック アトピー性皮膚炎適応追加WEB講演会in岡山  2021.11.10 

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  • 14例の長島型掌蹠角化症における、SERPINB7創始者変異のアレル頻度の解析

    伊藤靖敏, 武市拓也, 棚橋華奈, 吉川剛典, 村瀬友哉, 室 慶直, 池田賢太, 森実 真, 川上佳夫, 中村保夫, 清島真理子, 武藤 潤, 大磯直毅, 川田 暁, 杉浦一充, 須賀 康, 荻 朋男, 秋山真志

    第36回角化症研究会  2021.11.6 

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  • 当院における汎発性帯状疱疹と通常の帯状疱疹の臨床的検討

    浦上仁志, 三宅智子, 横山恵美, 山﨑 修, 森実 真

    第73回日本皮膚科学会西部支部学術大会  2021.10.30 

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  • 血管内リンパ腫を疑ったランダム皮膚生検の検討

    瀧川充希子, 野村隼人, 山﨑 修, 森実 真, 柳井広之

    第73回日本皮膚科学会西部支部学術大会  2021.10.30 

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  • 当院で経験したリンパ腫様丘疹省症11例の臨床的検討

    廣瀬 梓, 岡﨑布佐子, 梶田 藍, 山﨑 修, 森実 真

    第73回日本皮膚科学会西部支部学術大会  2021.10.30 

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  • 進行期に血小板減少を伴った頭部血管肉腫の3例

    佐藤志帆, 山﨑 修, 德田真優, 杉原 悟, 野村隼人, 森実 真

    第73回日本皮膚科学会西部支部学術大会  2021.10.30 

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  • 皮膚科医が行うPsA診療 Invited

    森実 真

    World Arthritis Day 特別企画  2021.10.16 

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  • 乾癬診療update2021~外用療法から生物学的製剤まで~ Invited

    森実 真

    Rising SUN Academy in OKAYAMA  2021.10.1 

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  • 特異疹が緊満性水疱を呈したATLLの1例

    妹尾春佳, 橋本倫子, 神野泰輔, 川上佳夫, 平井陽至, 谷口恒平, 松岡賢市, 山﨑 修, 森実 真

    第85回日本皮膚科学会東部支部学術大会  2021.9.18 

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  • 乾癬治療におけるIL17製剤の意義、トルツ使用経験と好適患者像について Invited

    森実 真

    Lilly Psoriasis Web Conference  2021.9.16 

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  • 当院における乾癬診療2021 Invited

    森実 真

    備後皮膚疾患勉強会  2021.9.16 

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  • 緩徐に進行するBRAF遺伝子変異陽性悪性黒色腫の1例

    竹﨑大輝, 立花宏太, 杉原 悟, 山﨑 修, 森実 真

    第284回日本皮膚科学会岡山地方会  2021.9.12 

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  • 当科における乾癬診療・研究とシムジアの位置付け Invited

    森実 真

    日本皮膚科学会第232回熊本地方会学術講演会 スポンサードセミナー  2021.9.12 

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  • カルシウム製剤の血管外漏出による医原性石灰沈着症の2例

    臼井真菜, 山﨑 修, 赤松由規, 森田安理, 杉原 悟, 三宅智子, 横山恵美, 森実 真

    第284回日本皮膚科学会岡山地方会  2021.9.12 

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  • 染毛剤の重合体が原因と考えられたアナフィラキシーの1例

    徳田真優, 三宅智子, 赤松由規, 前 琴絵, 芦田日美野, 山﨑 修, 森実 真, 松浦浩徳

    第284回日本皮膚科学会岡山地方会  2021.9.12 

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  • 尋常性乾癬の経過中に掌蹠膿疱症、間接症状を合併した1例

    竹﨑大輝, 瀧川充希子, 野村隼人, 森実 真, 岡﨑布佐子

    第36回日本乾癬学会学術大会  2021.9.3 

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  • 皮膚科医からみたPsAとJAK阻害剤 Invited

    森実 真

    リンヴォックPsA適応追加講演会in岡山  2021.8.27 

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  • 乾癬患者の爪症状を考える Invited

    森実 真, 多田弥生, 金子 栄

    実臨床における乾癬治療を考える会Online  2021.8.20 

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  • アトピー性皮膚炎治療の今後について考える Invited

    森実 真, 井川哲子

    AD Expert Conference in Asahikawa  2021.8.17 

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  • 岡山大学病院における診療連携2021~乾癬・アトピー専門外来を通じて~

    森実 真

    Psoriasis Seminar in 佐賀  2021.7.30 

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  • The analysis of the driver mutations in burn scar-related squamous cell carcinoma by whole-exome sequence

    Sugihara S, Yamasaki O, Morizane S, Tomida S

    2021 ASDR Annual Scientific Meeting  2021.7.29 

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  • Monokine induced by gamma interferon levels in plasma is a distinguishable marker between systemic and classical Hydroa vaccniforme

    Miyake T, Hirai Y, Yamamoto T, Nomura H, Iwatsuki K, Morizane S

    19th International Symposium on Epstein-Barr Virus and associated diseases  2021.7.29 

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  • 皮膚の痒み難治例について考える Invited

    森実 真, 村尾和俊, 下村尚子, 後藤寛之

    中四国皮膚のかゆみを考える会  2021.7.16 

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  • 繰り返す口唇腫脹を契機に診断に至った有毛細胞白血病の1例

    芦田日美野, 三宅智子, 住居優一, 立花宏太, 川上佳夫, 田中健大, 高橋孝英, 西餅久和, 吉野 正, 森実 真

    第37回日本皮膚悪性腫瘍学会学術大会  2021.7.9 

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  • BRAF遺伝子変異陽性の足の末端黒子型メラノーマの8例

    前 琴絵, 杉原 悟, 山﨑 修, 森実 真

    第37回日本皮膚悪性腫瘍学会学術大会  2021.7.9 

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  • 病理解剖にて判明した肛門管部原発の悪性黒色腫の1例

    杉原 悟, 藤井江利子, 中井友美, 野村隼人, 山﨑 修, 森実 真, 大原利章, 眞部恵子, 守山喬史

    第37回日本皮膚悪性腫瘍学会学術大会  2021.7.9 

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  • 2018207640 隆起性皮膚線維肉腫が疑われ、COL1A1-PDGFB融合遺伝子解析を行った5例

    日本皮膚科学会岡山地方会第273回例会  2018 

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  • 皮膚症状が長期に遷延するも免疫抑制剤の併用により軽快したDIHSの1例

    第47回日本皮膚アレルギー・接触皮膚炎学会総会学術大会  2017 

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  • 当科における皮膚動脈炎の予後不良因子の検討

    日本皮膚科学会第68回中部支部学術大会  2017 

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  • 表皮角化細胞産生セリンプロテアーゼ阻害因子の発現制御機構

    第22回日本病態プロテアーゼ学会学術集会  2017 

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  • アトピー性皮膚炎患者におけるSPINK5遺伝子変異解析

    第116回日本皮膚科学会総会  2017 

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  • 副腎皮質ホルモンおよび免疫抑制剤内服中に発症した皮膚非結核性抗酸菌症の2例

    第271回日本皮膚科学会岡山地方会  2017 

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  • 前脛骨型栄養障害型表皮水疱症と痒疹型栄養障害型表皮水疱症の位置づけ:genotype-phenotype解析の結果から

    第271回日本皮膚科学会岡山地方会  2017 

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  • New nonsynonymous variants of SPINK5 gene in Japanese atopic dermatitis patients

    76th Annual Meeting of Society of Investigative Dermatology  2017 

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  • 乾癬性関節炎の頻度と病型別最適治療の発見のための多施設共同観察調査(中間報告)

    日本皮膚科学会第79回沖縄地方会  2017 

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  • Double-stranded RNA enhances serine protease activities in epidermal keratinocytes

    The 42nd Annual Meeting of the Japanese Society for Investigative Dermatology  2017 

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  • 乾癬病変部角化細胞が産生するKey molecules

    阪神皮膚科カンファレンス  2016 

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  • The expression of serine protease inhibitors are induced by TNF-α and IL-17A in skin inflammatory diseases

    The 41st Annual Meeting of the Japanese Society for Investigative Dermatology  2016 

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  • TLR signaling regulates the expression of the expression of serine protease inhibitors in epidermal keratinocytes

    The 41st Annual Meeting of the Japanese Society for Investigative Dermatology  2016 

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  • The expression of serine protease inhibitors in epidermal keratinocytes is increased by calcium, but not 1,25(OH)2 vitamin D3 or retinoic acid

    The 41st Annual Meeting of the Japanese Society for Investigative Dermatology  2016 

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  • 抗セントロメア抗体陽性全身性強皮症患者に生じたサルコイドーシス HLA-DR解析と全身性ステロイド療法について

    日本皮膚科学会山口地方会第169回例会  2016 

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  • 抗PD-1抗体治療後BRAF阻害療法中の進行期メラノーマ患者に生じた原田病 抗PD-1投与前後の免疫応答を含めて

    日本皮膚科学会第98回大分地方会  2016 

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  • 進行性Sezary症候群の腫瘍細胞が発現する新規バイオマーカーCADM1の臨床的解析

    日本皮膚科学会第76回沖縄地方会  2016 

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  • アトピー性皮膚炎におけるセリンプロテアーゼインヒビターの発現および機能解析

    第46回日本皮膚アレルギー・接触皮膚炎学会総会学術大会  2016 

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  • Th2環境が表皮角化細胞産生カリクレインの発現および皮膚バリアに与える影響

    第46回日本皮膚アレルギー・接触皮膚炎学会総会学術大会  2016 

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  • 乾癬の診断と治療2016

    岡山PsA超音波実技講習会2016  2016 

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  • アトピー性皮膚炎におけるカリクレイン5および7の関与

    第21回日本病態プロテアーゼ学会学術集会  2016 

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  • 皮膚科医からみたPsA

    GRAPPA in 中四国 Internet Live Seminar  2016 

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  • 病院現況および患者視点からみた進行期悪性黒色腫に対する新規治療

    第115回日本皮膚科学会総会  2016 

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  • 帯状疱疹ウイルス・単純疱疹ウイルスの重複再活性化例の臨床及び遺伝子産物解析

    第115回日本皮膚科学会総会  2016 

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  • 非バイアス次世代TCRレパトア解析によるEBV関連リンパ増殖性疾患への応用

    第32回日本皮膚悪性腫瘍学会学術大会  2016 

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  • Pseudoxanthoma elasticum-like papillary dermal elastolysisの1例

    第32回日本臨床皮膚科医会総会・臨床学術大会  2016 

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  • 乾癬の診断と治療

    日本脊椎関節炎学会第25回学術大会  2015 

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  • 乾癬病変部角化細胞で産生される血清アミロイドA蛋白に関する研究

    第30回日本乾癬学会学術大会  2015 

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  • 複数の全身療法施行後に乳癌を発症した関節症性乾癬の1例

    第30回日本乾癬学会学術大会  2015 

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  • 岡山大学病院におけるセクキヌマブ使用症例

    Psoriasis&IL-17Aフォーラムin広島  2015 

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  • 経過中にリウマチ因子が陽転化し,抗TNF-a抗体,抗IL-12/IL-23p40抗体に治療抵抗性を示した関節症性乾癬の1例

    日本皮膚科学会第373回福岡地方会  2015 

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  • Inflammatory cytokines and antimicrobial peptides highly expressed in psoriatic lesions induce serum amyloid A expression in epidermal keratinocytes.

    2015 Australasian Society for Dermatology Research Conference  2015 

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  • Interferon-gamma enhances TLR3 expression and anti-viral activity in keratinocytes

    The 74th Annual Meeting of Society of Investigative Dermatology  2015 

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  • 乾癬の診断と治療~皮膚科医の立場から~

    第1回皮膚科膠原病内科合同グループワーク  2015 

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  • Distinct lymphocyte subsets responsible for classical and systemic hydroa vacciniforme

    The 40th Annual Meeting of the Japanese Society for Investigative Dermatology  2015 

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  • Cathelicidin peptide LL-37 induces UVB-triggered inflammasome activation and angiogenesis: Possible implications for rosacea

    The 40th Annual Meeting of the Japanese Society for Investigative Dermatology  2015 

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  • TNF-alpha, IL-17A, a Toll-like receptor 3 ligand, and high calcium enhances the expression of SPINK6 in epidermal keratinocytes

    The 40th Annual Meeting of the Japanese Society for Investigative Dermatology  2015 

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  • TNF-alpha and IL-17A increase SPINK5 expression in epidermal keratinocytes

    The 40th Annual Meeting of the Japanese Society for Investigative Dermatology  2015 

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  • Calcium but not 1,25(OH)2 vitamin D3 or retinoic acid increases SPINK5 expression in epidermal keratinocytes

    The 40th Annual Meeting of the Japanese Society for Investigative Dermatology  2015 

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  • Terminal Differentiation of Keratinocytes EnhancesTLR3 Expression and Anti-Viral Activity

    The 40th Annual Meeting of the Japanese Society for Investigative Dermatology  2015 

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  • 慢性炎症性皮膚疾患におけるセリンプロテアーゼ阻害因子の発現とその制御機構

    第113回日本皮膚科学会総会  2014 

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  • Chronic skin inflammation via Toll-like receptors increases a risk of secondary amyloidosis

    The 73rd Annual Meeting of the Society for Investigative Dermatology  2014 

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  • 当院におけるイミキモドクリームの適応拡大後の日光角化症治療について

    第261回日本皮膚科学会岡山地方会  2014 

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  • 躯幹に多発する暗紅色斑

    第65回日本皮膚科学会中部支部学術大会  2014 

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  • リュープロレリン酢酸塩皮下注射により生じた肉芽腫の2例

    第65回日本皮膚科学会中部支部学術大会  2014 

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  • 血液学的異常を伴ったfolliculotropic mycosis fungoidesについて

    第112回日本皮膚科学会総会  2013 

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  • Th2サイトカインはアトピー性皮膚炎患者における組織カリクレイン7の発現および機能を増強する

    第112回岡山医学会総会  2013 

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  • Eradication of cutaneous herpesvirus infections by nucleic acid sensors and cytotoxic T lymphocyte-mediated apoptotic processes

    2013 International Investigative Dermatology Meeting  2013 

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  • Pathogenic cell types, molecular mechanisms and prognostic factors in EB virus-associated cutaneous T/NK lymphoproliferative disorders

    2013 International Investigative Dermatology Meeting  2013 

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  • Toll-like receptor signaling induces serum amyloid A and IL-6 expression in patients with recessive dystrophic epidermolysis bullosa

    2013 International Investigative Dermatology Meeting  2013 

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  • 乾癬の内服療法~国内外の知見から~

    第112回日本皮膚科学会総会  2013 

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  • 岡山大学病院におけるウステキヌマブの使用症例の検討

    Psoriasis Meeting in Kagawa  2013 

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  • Cathelicidin(LL-37)は表皮角化細胞において2本鎖RNAによる抗ウィルス作用を増強する

    第9回中国研究皮膚科セミナー  2013 

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  • Inflammatory Cytokines Regulates Expression of Lympho-Epithelial Kazal-type-related Inhibitor in Epidermal Keratinocytes

    5th International Symposium on Kallikreins and Kallikrein-Related Peptidases  2013 

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  • Cahtelicidin antimicrobial peptide LL-37 enhances double-stranded RNA-induced IFN-beta expression and anti-viral activity in skin viral infection

    The 12th Awaji International Forum on Infection and Immunity  2013 

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  • 乾癬の理解と制御に向けた開発研究2013

    第28回日本乾癬学会学術大会  2013 

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  • 血液学的異常をともなった毛包向性菌状息肉症

    第29回日本皮膚悪性腫瘍学会学術大会  2013 

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  • アトピー性皮膚炎の治療を考える~TSLPの発現に与える影響~

    第10回小児皮膚・疾患フォーラム  2013 

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  • 日本乾癬学会”乾癬遺伝子プロジェクト班(第2期)”の発足

    第27回日本乾癬学会学術大会  2012 

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  • 乾癬と抗菌ペプチド

    第27回日本乾癬学会学術大会  2012 

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  • 非天疱瘡患者に検出される抗デスモグレイン前駆体抗体の特徴とEDTA処理ELISA法を用いた検討

    第111回日本皮膚科学会総会  2012 

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  • 上皮成長因子受容体阻害剤薬による遅発性皮膚障害

    第111回日本皮膚科学会総会  2012 

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  • Thymic stromal lymphopoietin expression in keratinocytes are suppressed by clinical treatments in atopic dermatitis

    The 72nd Annual Meeting of the Society for Investigative Dermatology  2012 

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  • Clues for Epstein-Barr virus reactivation with the subsequent outbreak of immune responses in skin lesions of hypersensitivity to mosquito bites.

    The 72nd Annual Meeting of the Society for Investigative Dermatology  2012 

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  • Widespread chronic skin ulcers in epidermolysis bullosa patients trigger Castleman's disease-like condition through Toll-like receptors

    The 72nd Annual Meeting of the Society for Investigative Dermatology  2012 

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  • Cathelicidin antimicrobial peptide LL-37 augments anti-viral activity induced by double-stranded RNA in keratinocytes

    The 37th Annual Meeting of the Japanese Society for Investigative Dermatology  2012 

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  • Interferon gamma enhances anti-viral activity induced by double-stranded RNA in keratinocytes

    The 37th Annual Meeting of the Japanese Society for Investigative Dermatology  2012 

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  • Clinical treatments for atopic dermatitis modulate thymic stromal lympopoietin expression in keratinocytes

    The 37th Annual Meeting of the Japanese Society for Investigative Dermatology  2012 

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  • 疣贅と伝染性軟属腫における免疫回避機序について

    第362回日本皮膚科学会福岡地方会  2012 

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  • Interferon gamma enhances anti-viral activity induced by double-stranded RNA in keratinocytes surrounding herpetic vesicles

    The 11th Awaji International Forum on Infection and Immunity  2012 

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  • An elderly case of crohn's disease with erythema nodosum

    22nd World Congress of Dermatology  2011 

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  • Granulysin-producing cytotoxic T cells in the mucocutaneous lesions of Behcet's disease: a distinct inflammatory response from erythema nodosum

    22nd World Congress of Dermatology  2011 

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  • Improved enzyme-linked immunosolvent assay to monitor pathogenic pemphigus antibodies directed against calcium-dependent epitopes of desmoglein 3.

    22nd World Congress of Dermatology  2011 

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  • Interferon gamma enhances TLR3 expression and function in keratinocytes in viral skin infection

    The 36th Annal Meeting of the Japanese Society for Investigative Dermatology  2011 

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  • Two Arg in C-terminal of human beta-defensin-3 are essential for the stimulation of proinflammatory cytokine secretion in keratinocytes

    The 36th Annal Meeting of the Japanese Society for Investigative Dermatology  2011 

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  • The epitopes and IgG subclasses of anti-desmoglein antibodies detected in individuals without pemphigus

    The 36th Annal Meeting of the Japanese Society for Investigative Dermatology  2011 

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  • Eccrine poromatosis associated with polychemotheraphy

    22nd World Congress of Dermatology  2011 

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  • 歯科金属除去が奏効した金属アレルギーによる異汗性湿疹

    第252回日本皮膚科学会岡山地方会  2011 

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  • Castleman's disease-like condition in dystrophic epidermolysis bullosa

    22nd World Congress of Dermatology  2011 

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  • 尋常性天疱瘡の疾患活動性とEDTA処理ELISA法で検出される病因性抗体値の比較検討

    第253回日本皮膚科学会岡山地方会  2011 

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  • Increased REG3A/RegIIIγ by Th17 cytokines promotes keratinocyte proliferation after skin injury.

    第71回米国皮膚科学会  2011 

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  • Differences in epitopes of non-pathogenic autoantibodies todesmoglein1 in pemphigus foliaceus in comparison with thosein pemphigus vulgaris and rheumatoid arthritis.

    第71回米国研究皮膚科学会  2011 

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  • Interferon gamma enhances innate immune response of epidermis

    第71回米国研究皮膚科学会  2011 

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  • EDTAおよび表皮剥脱毒素(ET)処理ELISA法による天疱瘡自己抗体のエピトープ解析

    第110回日本皮膚科学会総会  2011 

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  • 表皮水疱症における血液学的異常とキャッスルマン様病態

    第110回日本皮膚科学会総会  2011 

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  • 角化細胞カリクレイン発現および皮膚バリア

    アトピー性皮膚炎治療研究会第16回シンポジウム  2011 

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  • 多剤併用化学療法は長期間の潜伏を経て多発性エクリン汗孔腫を発症させる

    日本皮膚科学会第134回徳島地方会  2011 

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  • Hydroa vacciniforme is pathogenically associated with circulating Epstein-Barr virus-infected gammadelta T-cells

    The 36th Annal Meeting of the Japanese Society for Investigative Dermatology  2011 

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  • 尋常性天疱瘡患者の治療反応性に関する多様性は抗体産生細胞の反応性の違いに起因する

    第62回日本皮膚科学会中部支部学術大会  2011 

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  • アトピー性皮膚炎の表皮角化細胞における組織カリクレイン7の発現

    日本皮膚科学会愛媛地方会第54回学術大会  2011 

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  • Kallikrein expression in keratinocytes in atopic dermatitis

    Conférence du Service d’Immunologie Clinique et d’Allergologie  2011 

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  • Antimicrobial peptide LL37 in skin

    Jean Thivolet Seminar  2011 

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  • Interferon gamma enhances TLR3 expression and function in keratinocytes surrounding herpetic vesicles

    41st Annnual ESDR meeting  2011 

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  • 表皮水疱症に伴う血液学的合併症

    稀少難治性皮膚疾患に関する調査研究班平成23年度第1回総会  2011 

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  • Th2 cytokines enhance tissue kallikrein 7 expression and the serine protease activity of keratinocytes in atopic dermatitis

    第40回欧州研究皮膚科学会  2010 

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  • 乾癬病変部における抗菌ペプチドLL-37の発現と機能

    第25回日本乾癬学会学術大会  2010 

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  • 先天性表皮水疱症に伴う血液学的異常

    第34回日本小児皮膚科学会学術大会  2010 

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  • 四肢関節のみに分布する結節性紅斑から診断に至った高齢者Crohn病

    第250回日本皮膚科学会岡山地方会  2010 

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  • Th2 cytokines enhance tissue kallikreins and serine protease activity of keratinocytes in atopic dermatitis

    第70回米国研究皮膚科学会学術大会  2010 

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  • 抗菌タンパクCathelicidinのプロセッシング酵素カリクレインの発現制御機構

    第109回日本皮膚科学会総会  2010 

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  • ヘルペス性水疱の治癒に関わる細胞・分子メカニズム

    第109回日本皮膚科学会総会  2010 

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  • Hematological abnormalities associated with epidermolysis bullosa

    The First Eastern Asia Dermatology Congress  2010 

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  • Th2 cytokines enhance tissue kallikreins and serine protease activity of keratinocytes in atopic dermatitis

    The 70th annual meeting of Society for Investigative Dermatology  2010 

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  • アトピー性皮膚炎における角化細胞産生カリクレイン7の発現制御機構

    第40回日本皮膚アレルギー・接触皮膚炎学会総会学術大会  2010 

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  • 歯科金属除去が奏功した金属アレルギーによる異汗性湿疹の1例

    第40回日本皮膚アレルギー・接触皮膚炎学会総会学術大会  2010 

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  • Toll-like receptor signaling increases kallikrein in rosacea and affect skin barrier function

    日本研究皮膚科学会 第35回年次学術大会・総会  2010 

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  • A simple method to detect pathogenic pemphigus antibodies directed against calcium-dependent epitopes of desmoglein 3

    日本研究皮膚科学会 第35回年次学術大会・総会  2010 

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  • Tissue kallikrein 7 expression and the protease activity in keratinocytes are enhanced by Th2 cytokines in atopic dermatitis

    日本研究皮膚科学会 第35回年次学術大会・総会  2010 

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  • 先天性表皮水疱症における血液学的異常

    第32回水疱症研究会  2010 

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  • 天疱瘡患者の病原性抗体をより高感度に検出するEDTA処理ELISA法の検討

    第32回水疱症研究会  2010 

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  • Hematological abnormalities associated with epidermolysis bullosa

    The First Eastern Asia Dermatology Congress  2010 

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  • The antimicrobial peptide LL37 in psoriasis enhances TLR9 responsiveness and increases IL-23 expression in keratinocytes

    日本研究皮膚科学会第34回年次学術大会・総会  2009 

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  • 抗菌ペプチドLL-37は乾癬でTLR9の機能を増強し、IL-23の産生を誘導する

    第5回中国研究皮膚科セミナー  2009 

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  • 皮膚における抗菌ペプチドLL-37の免疫修飾機能とそのプロセッシング酵素カリクレインの発現調節機構

    第22回岡山研究皮膚科フォーラム  2009 

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  • LL37 enhances TLR9 expression and activation in keratinocytes and is coordinately overexpressed in psoriasis

    第69回米国研究皮膚科学会学術大会  2009 

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  • LL37 enhances TLR9 expression and activation in keratinocytes and is coordinately overexpressed in psoriasis

    Gordon Research Conference on Antimicrobial Peptides  2009 

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  • The antimicrobial peptide LL37 in psoriasis enhances TLR9 responsiveness and increases IL-23 expression in keratinocytes

    2009 

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  • LL37 enhances TLR9 expression and activation in keratinocytes and is coordinately overexpressed in psoriasis

    The 69th annual meeting of the society for investigative dermatology  2009 

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  • 皮膚抗菌バリアと自然免疫応答・アレルギー

    第3回病態制御科学専攻系シンポジウム  2009 

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  • ヘルペス性水疱におけるアポトーシス誘導因子(免疫不全状態との比較)

    第243回日本皮膚科学会岡山地方会  2008 

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  • Panton-Valentine leukocidin produced by Staphylococcus aureus induces an intense inflammation in the skin

    第68回米国研究皮膚科学会学術大会  2007 

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  • Cytotoxic T lymphocyte(CTL)-mediated vasculopathy

    第144回日本皮膚科学会山口地方会  2007 

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Awards

  • 第4回高木賞

    2020.3  

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  • 2018年度公募課題研究助成金(リディアオリリー記念ピアス皮膚科学振興財団)

    2018.4  

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  • 公益財団法人岡山医学振興会第16回教育・研究助成

    2016.11  

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  • ASDR/JSID Exchange Program

    2015  

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    Country:Japan

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  • JD Award “Most cited paper in 2014 Impact Factor period”

    2015  

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    Country:Japan

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  • 第4回2014年日本乾癬学会「鳥居・帝國乾癬研究奨励賞」

    2014.9  

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  • JD Award “Most downloaded paper in 2013”

    2014  

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    Country:Japan

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  • JD Award “Most cited paper in 2013 Impact Factor period”

    2014  

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    Country:Japan

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  • 平成25年度日本皮膚科学会基礎医学研究費(資生堂寄附)

    2013.4  

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  • 平成24年度岡山医学会賞(結城賞)

    2013  

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    Country:Japan

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  • 2011年度財団法人日本リディアオリリー協会研究助成金

    2011.5  

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  • EADC Award

    2010  

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    Country:Japan

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  • 第109回日本皮膚科学会総会ポスター賞

    2010  

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    Country:Japan

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Research Projects

  • Elucidation of the pathogenesis of Netherton syndrome using model mice

    Grant number:23K07766  2023.04 - 2026.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    森実 真

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    Grant amount:\4810000 ( Direct expense: \3700000 、 Indirect expense:\1110000 )

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  • 高深度プロテオーム解析による悪性黒色腫血中腫瘍マーカーの探索と臨床応用

    Grant number:23K07796  2023.04 - 2026.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    梅村 啓史, 川島 祐介, 森実 真, 山崎 修, 土田 祥央

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    Grant amount:\4680000 ( Direct expense: \3600000 、 Indirect expense:\1080000 )

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  • Mechanisms of reciprocal regulation of immune diseases by EBI3-containing heterodimer cytokines

    Grant number:23K07917  2023.04 - 2026.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    井関 將典, 向井 知之, 森実 真

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

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  • 乾癬病変部表皮角化細胞産生EBI3含有サイトカインに関する研究

    Grant number:20K08672  2020.04 - 2023.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    森実 真

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    乾癬患者皮膚病変部、あるいは皮膚腫瘍手術標本断端のパラフィンブロック標本を用いて、EBI3の発現を免疫染色法によって検討したところ、EBI3は乾癬病変部の表皮にて全層性に高発現していることが確認された。
    培養ヒト正常表皮角化細胞を炎症性サイトカイン(TNF-alpha+IFN-gamma+IL-17A、いずれも50ng/ml)で24~96時間刺激後、培養上清を遠心式限外ろ過フィルターにて25~80倍濃縮し、一次抗体(抗EBI3抗体)と4℃一晩インキュベート。Protein Aアガロースビーズ処理後、PBSで洗浄し、0.1mol/l グリシン-塩酸緩衝液 (pH2.2) で懸濁し、結合蛋白質を溶出。その後トリプシン消化(Trypsin、37℃、一晩)し、nano LC-MS/MSによる受託プロテオーム解析を施行したところ、EBI3蛋白を検出することが出来たが、p19、p28、p35は検出されなかった。これらの結果からは表皮角化細胞がIL-27、IL-35、IL-39を産生しているとは考えにくく、EBI3モノマー・ホモダイマー・ホモトリマーあるいは未知のヘテロダイマーなどを産生している可能性が示唆された。
    以上の結果をInternational Journal of Molecular Sciences誌に報告した。

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  • the elucidation of complication mechanism in patients with recessive dystrophic epidermolysis bullosa (RDEB)

    Grant number:16K19724  2016.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Tenta Ai, MORIZANE Shin

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    Grant amount:\3900000 ( Direct expense: \3000000 、 Indirect expense:\900000 )

    We found that patients with recessive dystrophic epidermolysis bullosa (RDEB) have persistently higher levels of serum amyloid A (SAA) and IL-6 in the serum. Furthermore, we demonstrated that Toll-like receptor (TLR) ligands induce SAA expression via NF-kB in epidermal keratinocytes and dermal fibroblasts, and that SAA induces its own expression via TLR1/2 in these cells. Our results provide new evidence that the skin’s innate immune response contributes to the production of SAA, which might lead to an increased risk of systemic complications such as secondary amyloidosis of recessive dystrophic epidermolysis bullosa.

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  • The analysis of regulation of serine protease inhibitors expression in epidermal keratinocytes

    Grant number:26461658  2014.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Morizane Shin

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    Grant amount:\4940000 ( Direct expense: \3800000 、 Indirect expense:\1140000 )

    High calucium, TNF-alpha, IL-17A, and Toll-like receptor ligands significantly inudced the expression of LEKTI, SLPI, and elafin in normal human epidermal keratinocytes. In addition, high calcium up- and down-regulated trypsin- and chymotrypsin-like serine protease activities in epidermal keratinocytes, respectively.
    Immunohistochemical analysis revealed that the expression of LEKTI, SLPI, and elafin were increased in the lesion of psoriasis, rosacea, acne, chronic pyoderma, and herpes zoster.

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  • Studies on host immune responses induced by reactivation of EB virus-infected lymphocytes and resultant skin lesions

    Grant number:24591653  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    IWATSUKI Keiji, YAMAMOTO Takenobu, MORIZANE Shin

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    Grant amount:\5330000 ( Direct expense: \4100000 、 Indirect expense:\1230000 )

    We performed diagnostic examinations on more than 50 cases of EB virus-associated T/NK lymphoproliferative disorders including hydroa vacciniforme (HV), hypersensitivity to mosquito bites (HMB), chronic active EB virus infections(CAEBV) and so on, and studied the pathogenesis. EB virus was found to be infected mainly in gdT cells in classical HV, abT cells in systemic HV, and NK cells in HMB. These results indicate that EB virus-infected lymphocyte subsets are closely related to the clinical phenotypes.
    We found prognostic factors in our series of patients: clinical phenotypes, age of the disease onset, and the expression of EB virus reactivation marker, BZLF1. The results suggest that host immune responses against the EBV antigens expressed in the skin lesions induce the severe clinical symptoms. Furthermore, we found elderly patients with atypical HV, and reported their cytological characteristics and EB virus gene expression patterns.

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  • The effect of aberrant expression of kallikrein 7 induced by Th2 cytokines on epidermal barrier

    Grant number:24791157  2012.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    MORIZANE Shin

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    TNF-alpha and IL-17A significantly up-regulated the expression of LEKTI, SLPI, and elafin in cultured normal human epidermal keratinocytes. Immunohistochemical analysis confirmed LEKTI expression in atopic dermatitis lesions was almost same as in normal skin, but it was significantly higher in psoriasis lesions than in atopic dermatitis lesions.
    Topical application of serine protease inhibitors such as benzamidine did not significantly improve the skin lesions in a mouse model of Th2-dependent dermatitis.

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  • Investigation of expression regulation and pathological mechanism of Panton-valentine leukocidine in skin

    Grant number:23591646  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    YAMASAKI Osamu, MORIZANE Shin, KANEKO Jun

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    Grant amount:\5200000 ( Direct expense: \4000000 、 Indirect expense:\1200000 )

    Panton-Valentine leukocidin (PVL) is a cytotoxin produced by Staphylococcus aureus, PVL exhibits very highly specific lytic activity against polymorphonuclear cells of human and rabbits. PVL gene-positive S. aureus is strongly associated with furunculosis and community-acquired necrotic pneumonia. We demonstrated PVL gene-positive S. aureus strains are involved in the development of multiple furuncles with more intense erythema, particularly in healthy young adults. However, the role of PVL in the development of furunculosis has not been fully elucidated. We analyzed regulatory factors of PVL toxin production and of the influence of PVL on keratinocytes, fibroblasts and endothelial cells. In addition, we investigated about PVL-carrying phage type in furunculosis.

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  • Preferable biological milieu for the growth of viral epithelial neoplasms and its regulation

    Grant number:22659205  2010 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    IWATSUKI Keiji, AOYAMA Yumi, SHIRAFUJI Yoshinori, FUJII Kazuyasu, MORIZANA Shin

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    Grant amount:\3230000 ( Direct expense: \2900000 、 Indirect expense:\330000 )

    Dendritic cell(DC) subsets and regulatory mechanisms of host immune responses were studied in human papilloma virus(HPV)-and molluscum contagiosum virus(MCV)-associated warty lesions, Bowen disease, and various epithelial neoplasms. The depletion of Langerin+ DCs(LCs) was obvious in the non-inflammatory HPV-warts, MC, and HPV-Bowen. The lesional epidermis revealed diminished MIP-3αexpression and irregular distribution of E-cadherin. TSLP(thymic stromal lymphopoietin) was highly expressed in HPV-warts and solar keratosis, while a few MCV+ cells were positive for TSLP in MC. MCV-encoded immunoregulatory molecules such as MCV148 and MCV159 were expressed in MC. MIP-3αexpression is up-regulated in inflammatory warts, with the subsequent recruitment of various DC subsets and CD8+ cells. TSLP expression might be associated with immune suppression and Th2 type inflammation in HPV-warts, while MCV-encoded molecules possess a pivotal role in the persistence of MC.

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  • An effect of Th2 environment on kallikrein expression in keratinocytes and skin barrier

    Grant number:22791074  2010 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    MORIZANE Shin

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    Grant amount:\4030000 ( Direct expense: \3100000 、 Indirect expense:\930000 )

    We found that Th2 cytokines, IL-4 and IL-13, increase kallikrein 7 expression and function in keratinocytes. Further, KLK7 protein level in the sera of atopic dermatitis patients significantly correlated with IL-4 amounts in the sera. Our finding describes a link between the Th2 environment and epidermal barrier dysfunction in atopic dermatitis.

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Class subject in charge

  • Introduction to Medical Science (Medicine and Life) (2023academic year) Third semester  - 木5~6

  • Research Presentation in Pathophysiology (2023academic year) special  - その他

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  • Introduction to Clinical Medicine and Dentistry (2023academic year) Concentration  - その他

  • Elective Clinical Practice (Dermatology) (2023academic year) special  - その他

  • Elective Clinical Practice (Dermatology) (2023academic year) special  - その他

  • Research Presentation in Pathophysiology (2022academic year) special  - その他

  • Dermatology (Core Clinical Practice) (2022academic year) special  - その他

  • Research Projects and Practicals: Dermatology I (2022academic year) special  - その他

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  • Lecture and Research Projects: Dermatology II (2022academic year) special  - その他

  • Dermatology (2022academic year) special  - その他

  • Introduction to Clinical Medicine and Dentistry (2022academic year) Concentration  - その他

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  • Elective Clinical Practice (Dermatology) (2022academic year) special  - その他

  • Research Presentation in Pathophysiology (2021academic year) special  - その他

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  • Research Projects and Practicals: Dermatology II (2021academic year) special  - その他

  • Lecture and Research Projects: Dermatology II (2021academic year) special  - その他

  • Dermatology (2021academic year) special  - その他

  • Introduction to Clinical Medicine and Dentistry (2021academic year) Concentration  - その他

  • Elective Clinical Practice (Dermatology) (2021academic year) special  - その他

  • Elective Clinical Practice (Dermatology) (2021academic year) special  - その他

  • Research Presentation in Pathophysiology (2020academic year) Year-round  - その他

  • Pathophysiology (2020academic year) Year-round  - その他

  • Dermatology (Core Clinical Practice) (2020academic year) special  - その他

  • Research Projects and Practicals: Dermatology I (2020academic year) special  - その他

  • Lecture and Research Projects: Dermatology I (2020academic year) special  - その他

  • Research Projects and Practicals: Dermatology II (2020academic year) special  - その他

  • Lecture and Research Projects: Dermatology II (2020academic year) special  - その他

  • Dermatology (2020academic year) special  - その他

  • Introduction to Clinical Medicine and Dentistry (2020academic year) Concentration  - その他

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