研究者詳細 - 百田　龍輔

論文 - 百田　龍輔

分割表示 53 件中 41 - 53 件目／全件表示 >>

マウス成長軟骨におけるADAMTS-9発現の検討

熊岸加苗, 西田圭一郎, 百田龍輔, 山合友一朗, 広畑聡, Demircan Kadir, 内藤一郎, 二宮善文, 大塚愛二

解剖学雑誌 82 ( Suppl. ) 150 - 150 2007年3月

IV型コラーゲンα鎖特異抗体反応基の抗原賦活法

森雄一, 内藤一郎, 百田龍輔, 二宮善文, 大塚愛二

解剖学雑誌 82 ( Suppl. ) 271 - 271 2007年3月

ヒト赤血球には内部細胞骨格が見られ、βアクチンとニューロフィラメント蛋白を含む

寺沢和貴, 田口勇仁, 百田龍輔, 内藤一郎, 大塚愛二

解剖学雑誌 82 ( Suppl. ) 231 - 231 2007年3月

ラットの腹側尾動脈の血管鋳型標本に見られた風変わりな刻印

品岡玲, 百田龍輔, 大塚愛二, 内藤一郎

解剖学雑誌 82 ( Suppl. ) 150 - 150 2007年3月

Human erythrocytes possess a cytoplasmic endoskeleton containing beta-actin and neurofilament protein 査読

Kazutaka Terasawa, Takehito Taguchi, Ryusuke Momota, Ichiro Naito, Takuro Murakami, Aiji Ohtsuka

ARCHIVES OF HISTOLOGY AND CYTOLOGY 69 ( 5 ) 329 - 340 2006年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：INT SOC HISTOLOGY & CYTOLOGY

The biconcave disc shape of mammalian erythrocytes has been considered to be maintained only with a membrane underlain by a membranous cytoskeleton. Our improved ion-etching/scanning electron microscopy and saponin-ethanol treatment combined with immunocytochemistry in the human red blood cell revealed the three-dimensional structure of this cytoplasmic endoskeleton apart from the classical membranous cytoskeleton. The endoskeletal meshwork images obtained by the saponin-ethanol treatment corresponded to those by the repeated ion-etching method. The actin-rich endoskeleton was divided into two layers, one superficial and the other deep. The superficial filaments were perpendicularly connected to the membranous cytoskeleton, while the deep filaments formed an irregularly directed complicated meshwork. In the transitional hillside region between the convex periphery and concave center, the endoskeletal filaments containing a neurofilament protein ran parallel to the hillside slope toward the concave center. The endoskeleton of the erythrocyte associating with the membranous cytoskeleton may serve to keep its unique biconcave disc shape deformable, pliable, and restorable against external circumstances.

DOI： 10.1679/aohc.69.329

Web of Science

researchmap

ADAMTS-ECM interaction modulates BMP developmental control 査読

J. Fessler, R. Momota, C. Cresse, K. Chavan, L. Fessler

MATRIX BIOLOGY 25 S30 - S30 2006年11月

血管基底膜IV型コラーゲンの免疫組織学的解析共焦点レーザー顕微鏡を用いた三次元観察

森本尊雅, 内藤一郎, 百田龍輔, 芳原成美, 長谷川治子, 田口勇仁, 西田圭一郎, 佐渡義一, 二宮善文, 大塚愛二

解剖学雑誌 81 ( Suppl. ) 155 - 155 2006年3月

基底膜の多様性 α(IV)鎖特異抗体をもちいた基底膜IV型コラーゲンα鎖の多様性解析

内藤一郎, 長谷川治子, 森本尊雅, 百田龍輔, 斉藤健司, 佐渡義一, 二宮善文, 大塚愛二

解剖学雑誌 81 ( Suppl. ) 110 - 110 2006年3月

In situ preparation of colloidal iron by microwave irradiation for transmission electron microscopy 査読

S Nakatani, Naito, I, R Momota, N Hinenoya, K Horiuchi, K Nishida, A Ohtsuka

ACTA MEDICA OKAYAMA 60 ( 1 ) 59 - 64 2006年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OKAYAMA UNIV MED SCHOOL

We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.

Web of Science

researchmap

In situ preparation of colloidal iron by microwave irradiation for transmission electron microscopy 査読

S Nakatani, Naito, I, R Momota, N Hinenoya, K Horiuchi, K Nishida, A Ohtsuka

ACTA MEDICA OKAYAMA 60 ( 1 ) 59 - 64 2006年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OKAYAMA UNIV MED SCHOOL

DOI： 10.18926/AMO/30753

Web of Science

PubMed

researchmap

Differential expression of mouse alpha 5(IV) and alpha 6(IV) collagen genes in epithelial basement membranes 査読

K Saito, Naito, I, T Seki, T Oohashi, E Kimura, R Momota, Y Kishiro, Y Sado, H Yoshioka, Y Ninomiya

JOURNAL OF BIOCHEMISTRY 128 ( 3 ) 427 - 434 2000年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE BIOCHEMICAL SOC

We first completed the primary structure of the mouse alpha 5(IV) and alpha 6(IV) chains, from which synthetic peptides were produced and alpha chain-specific monoclonal antibodies were raised. Expression of collagen IV genes in various basement membranes underlying specific organ epithelia was analyzed by immunohistochemical staining using these monoclonal antibodies and other antibodies from human and bovine sequences. It was possible to predict the presence of the three collagen TV molecules: [alpha 1(IV)](2) alpha 2(IV), alpha 3(IV)alpha 4(IV)alpha 5(IV), and [alpha 5(IV)](2)alpha 6(IV), In skin basement membrane two of the three forms, [alpha 1(IV)](2)alpha 2(IV) and [alpha 5(IV)](2)alpha 6(IV), were detected. The alpha 3(IV)alpha 4(IV)alpha 5(IV) molecule was observed as the major form in glomerulus, alveolus, and choroid plexus, where basement membranes function as filtering units. The molecular form [alpha 5(IV)](2)alpha 6(IV) was present in basement membranes in tubular organs such as the epididymis, where the tubes need to expand in diameter. Thus, the distribution of the basement membranes with different molecular composition is consistent with tissue-specific function.

Web of Science

researchmap

Organization and expression of basement membrane collagen IV genes and their roles in human disorders 査読

Y Sado, M Kagawa, Naito, I, Y Ueki, T Seki, R Momota, T Oohashi, Y Ninomiya

JOURNAL OF BIOCHEMISTRY 123 ( 5 ) 767 - 776 1998年5月

記述言語：英語出版者・発行元：JAPANESE BIOCHEMICAL SOC

Six distinct genes have been identified as belonging to the type IV collagen gene family. They can be organized into three sets, i.e., COL4A1/COL4A2, COL4A3/COL4A4, and COL4A5/COL4A6, which are localized on three different chromosomes in humans, 13, 2, and X, respectively, Within each set the genes are aligned head-to-head and their expression is regulated by bidirectional promoters between the genes. Transcriptional regulation of the COL4A1/COL4A2 set has been well characterized, The transcription of COL4A6 seems to be controlled by two alternative promoters. While collagen IV molecules composed of alpha 1 and alpha 2 chains are broadly distributed, molecules comprising combinations of the other four chains, alpha 3-alpha 6, are important components of specialized basement membranes, The precise chain composition of triple-helical molecules assembled from the alpha 3-alpha 6 chains is not entirely clear, but it is hypothesized that alpha 3-alpha 5 chains and alpha 5 and alpha 6 chains form heterotrimeric molecules. Several pieces of evidence indicate that alpha 3/alpha 4/alpha 5 molecules and alpha 5/alpha 6 molecules are components of the basement membrane network, This helps explain the observation that the kidney and skin basement membranes from patients with Alport syndrome caused by mutations in the alpha 5 coding gene, COL4A5, are defective in the alpha 3, alpha 4, and alpha 6 chains together with the alpha 5 chain. Large deletions involving the COL4A5 and COL4A6 genes have been found in rare cases of diffuse leiomyomatosis associated with Alport syndrome.

Web of Science

researchmap

Two genes, COL4A3 and COL4A4 coding for the human alpha 3(IV) and alpha 4(IV) collagen chains are arranged head-to-head on chromosome 2q36 査読

R Momota, M Sugimoto, T Oohashi, K Kigasawa, H Yoshioka, Y Ninomiya

FEBS LETTERS 424 ( 1-2 ) 11 - 16 1998年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

We first isolated and characterized genomic DNA fragments that cover the 5' flanking sequences of COL4A3 and COL4A4 encoding the human basement membrane alpha 3(IV) and alpha 4(IV) collagen chains, respectively. Nucleotide sequence analysis indicated that the two genes are arranged head-to-head. To determine transcription start site for COL4A4 gene, we performed RACE and RNase protection assays, indicating that there are two alternative transcripts presumably derived from two different promoters. Interestingly, one transcription start site (from exon 1') of COL4A4 is only 5 bp away from the reported transcription start site of COL4A3, whereas the other transcript (from exon 1) starts 373 nucleotides downstream from the first one, generating the two kinds of transcripts that differ in the 5' UTR regions. Expression of these two transcripts appears tissue-specific; exon 1 transcript was expressed predominantly in epithelial cells, while exon 1' transcript showed rather ubiquitous and low expression. The nucleotide sequence of the promoter region is composed of dense CpG dinucleotides, GC boxes, CTC boxes and a CCAAT box but no TATA box. These results provide information to delineate the promoter activity for the tissue-specific expression of the six type IV collagen genes and basement membrane assembly in different tissues and organs. (C) 1998 Federation of European Biochemical Societies.

DOI： 10.1016/S0014-5793(98)00128-8

Web of Science

researchmap

　前のページ - 次のページ　

<百田 龍輔>

論文 - 百田 龍輔

<百田　龍輔>

論文 - 百田　龍輔