Faculty Profiles - MOMOTA Ryusuke

Papers - MOMOTA Ryusuke

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長鎖型XVIII型コラーゲンは新規膜結合型コラーゲンである可能性が高い

上野智規, 米澤朋子, 百田龍輔, 佐々木隆子, 大橋俊孝, 水野一乘

日本結合組織学会学術大会プログラム・抄録集 55回 147 - 147 2023.6

XVIII型コラーゲン欠損マウスにおける皮膚創傷治癒の解析

米澤朋子, 前川明日華, 前場崇宏, 百田龍輔, 渋谷千晶, 岩田宗一郎, 大野充昭, 大橋俊孝

日本結合組織学会学術大会プログラム・抄録集 54回 119 - 119 2022.6

Lack of collagen α6(IV) chain in mice does not cause severe-to-profound hearing loss or cochlear malformation, a distinct phenotype from nonsyndromic hearing loss with COL4A6 missense mutation. International journal

Shaoying Tang, Tomoko Yonezawa, Yukihide Maeda, Mitsuaki Ono, Takahiro Maeba, Toru Miyoshi, Ryusuke Momota, Yasuko Tomono, Toshitaka Oohashi

PloS one 16 ( 4 ) e0249909 2021

Language：English Publishing type：Research paper (scientific journal)

Congenital hearing loss affects 1 in every 1000 births, with genetic mutations contributing to more than 50% of all cases. X-linked nonsyndromic hereditary hearing loss is associated with six loci (DFNX1-6) and five genes. Recently, the missense mutation (c.1771G>A, p.Gly591Ser) in COL4A6, encoding the basement membrane (BM) collagen α6(IV) chain, was shown to be associated with X-linked congenital nonsyndromic hearing loss with cochlear malformation. However, the mechanism by which the COL4A6 mutation impacts hereditary hearing loss has not yet been elucidated. Herein, we investigated Col4a6 knockout (KO) effects on hearing function and cochlear formation in mice. Immunohistochemistry showed that the collagen α6(IV) chain was distributed throughout the mouse cochlea within subepithelial BMs underlying the interdental cells, inner sulcus cells, basilar membrane, outer sulcus cells, root cells, Reissner's membrane, and perivascular BMs in the spiral limbus, spiral ligament, and stria vascularis. However, the click-evoked auditory brainstem response analysis did not show significant changes in the hearing threshold of Col4a6 KO mice compared with wild-type (WT) mice with the same genetic background. In addition, the cochlear structures of Col4a6 KO mice did not exhibit morphological alterations, according to the results of high-resolution micro-computed tomography and histology. Hence, loss of Col4a6 gene expression in mice showed normal click ABR thresholds and normal cochlear formation, which differs from humans with the COL4A6 missense mutation c.1771G>A, p.Gly591Ser. Therefore, the deleterious effects in the auditory system caused by the missense mutation in COL4A6 are likely due to the dominant-negative effects of the α6(IV) chain and/or α5α6α5(IV) heterotrimer with an aberrant structure that would not occur in cases with loss of gene expression.

DOI： 10.1371/journal.pone.0249909

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コラーゲン研究の新展開:基礎から創薬までマウス皮膚創傷モデルにおけるXVIII型コラーゲンの解析

米澤朋子, 前場崇宏, Tang Shaoying, 大野充昭, 百田龍輔, 稲川喜一, 大橋俊孝

日本生化学会大会プログラム・講演要旨集 93回 [1S08e - 06] 2020.9

Prolonged Tachycardia with Higher Heart Rate Is Associated with Higher ICU and In-hospital Mortality. Reviewed

Hayashi M, Taniguchi A, Kaku R, Fujimoto S, Isoyama S, Manabe S, Yoshida T, Suzuki S, Shimizu K, Morimatsu H, Momota R

Acta medica Okayama 73 ( 2 ) 147 - 153 2019.4

Language：English Publishing type：Research paper (scientific journal)

Tachycardia is common in intensive care units (ICUs). It is unknown whether tachycardia or prolonged tachycardia affects patient outcomes. We investigated the association between tachycardia and mortality in critically ill patients. This retrospective cohort study's primary outcome was patient mortality in the ICU and the hospital. We stratified the patients (n=476) by heart rate (HR) as LowHR, MediumHR, and HighHR groups. We also stratified them by their durations of HR >100 (prolonged HR; tachycardia): MildT, ModerateT, and SevereT groups. We determined the six groups' mortality. The ICU mortality rates of the LowHR, MediumHR, and HighHR groups were 1.0%, 1.5%, and 7.9%, respectively; significantly higher in the HighHR vs. LowHR group. The in-hospital mortality rates of these groups were 1%, 4.5%, and 14.6%, respectively; significantly higher in the HighHR vs. LowHR group. The ICU mortality rates of the MildT, ModerateT, and SevereT groups were 0.9%, 5.6%, and 57.1%, respectively. The mortality of the HRT=0 (i.e., all HR ≤ 100) patients was 0%. The in-hospital mortality rates of the MildT, ModerateT, and SevereT groups were 1.8%, 16.7%, and 85.7%, respectively; that of the HRT=0 patients was 0.5%. Both higher HR and prolonged tachycardia were associated with poor outcomes.

DOI： 10.18926/AMO/56650

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Unripe peach (Prunus persica) extract ameliorates damage from UV irradiation and improved collagen XVIII expression in 3D skin model. Reviewed International journal

Tomoko Yonezawa, Ryusuke Momota, Hideki Iwano, Steven Zhao, Tomohiro Hakozaki, Chieko Soh, Shigetoyo Sawaki, Kazumi Toyama, Toshitaka Oohashi

Journal of cosmetic dermatology 2018.12

Language：English Publishing type：Research paper (scientific journal)

INTRODUCTION: Collagen type XVIII regulates cellular activities of adjacent cells at the dermal-epidermal junction (DEJ). To investigate its possible changes during aging, we compared its mRNA levels and protein localization in skin samples from female participants aged 20-70 years old. In addition, we evaluated the beneficial effects of unripe peach extracts in a 3D skin model. METHODS: Sun-exposed or sun-protected female skin samples were compared by DNA array or by immunohistochemistry for basement membrane components. To evaluate protective effects of fresh unripe peach extract, UV-B irradiated human 3D skin models were incubated in the presence or absence of the extract, followed by measurements of mRNA levels by real-time PCR, or by immunohistochemistry. RESULTS: In aged skin samples, COL18A1 mRNA levels were lower and the protein localization exhibited less intensive signal by anti-collagen type XVIII immunostaining. As observed in the skin tissues, collagen type XVIII exists at the DEJ in the 3D skin model. Fresh unripe peach extract significantly improved mRNA levels and partially localizations of collagen type XVIII, suggesting that fresh unripe peach extract ameliorates DEJ damages caused by UV-B irradiation. CONCLUSION: Collagen type XVIII and fresh unripe peach extract can be promising protective cosmetic strategies against skin aging.

DOI： 10.1111/jocd.12841

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Network of anatomical texts (NAnaTex), an open-source project for visualizing the interaction between anatomical terms Reviewed

Ryusuke Momota, Aiji Ohtsuka

Anatomical Science International 93 ( 1 ) 149 - 153 2018.1

Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Tokyo

Anatomy is the science and art of understanding the structure of the body and its components in relation to the functions of the whole-body system. Medicine is based on a deep understanding of anatomy, but quite a few introductory-level learners are overwhelmed by the sheer amount of anatomical terminology that must be understood, so they regard anatomy as a dull and dense subject. To help them learn anatomical terms in a more contextual way, we started a new open-source project, the Network of Anatomical Texts (NAnaTex), which visualizes relationships of body components by integrating text-based anatomical information using Cytoscape, a network visualization software platform. Here, we present a network of bones and muscles produced from literature descriptions. As this network is primarily text-based and does not require any programming knowledge, it is easy to implement new functions or provide extra information by making changes to the original text files. To facilitate collaborations, we deposited the source code files for the network into the GitHub repository (https://github.com/ryusukemomota/nanatex) so that anybody can participate in the evolution of the network and use it for their own non-profit purposes. This project should help not only introductory-level learners but also professional medical practitioners, who could use it as a quick reference.

DOI： 10.1007/s12565-017-0410-1

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Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium Reviewed International journal

Komori, T., Ono, M., Hara, E.S., Ueda, J., Nguyen, H.T.T., Nguyen, H.T., Yonezawa, T., Maeba, T., Kimura-Ono, A., Takarada, T., Momota, R., Maekawa, K., Kuboki, T., Oohashi, T.

Scientific Reports 8 ( 1 ) 2612 - 2612 2018

Language：English Publishing type：Research paper (scientific journal)

Keratinized mucosa is of fundamental importance to maintain healthy gingival tissue, and understanding the mechanisms of oral mucosa keratinization is crucial to successfully manage healthy gingiva. Previous studies have shown a strong involvement of the basement membrane in the proliferation and differentiation of epithelial cells. Therefore, first, to identify the keratinized mucosa-specific basement membrane components, immunohistochemical analysis for the six alpha chains of type IV collagen was performed in 8-week-old mice. No difference in the expression pattern of type IV collagen α1(IV) and α2(IV) chains was observed in the keratinized and non-keratinized mucosa. Interestingly, however, type IV collagen α5(IV) and α6(IV) chains specifically were strongly detected in the keratinized mucosa. To analyze the functional roles of the type IV collagen isoform α6(IV) in oral mucosa keratinization, we analyzed Col4a6-knockout mice. Epithelial developmental delay and low levels of KRT10 were observed in new-born Col4a6-knockout mice. Additionally, in vitro experiments with loss-of function analysis using human gingival epithelial cells confirmed the important role of α6(IV) chain in epithelial keratinization. These findings indicate that α112:α556 (IV) network, which is the only network that includes the α6(IV) chain, is one regulator of KRT10 expression in keratinization of oral mucosal epithelium.

DOI： 10.1038/s41598-018-21000-0

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Effects of aerobic exercise on capillary architecture of extensor digitorum Longus in Rat Reviewed

Shinichiro Murakami, Masahiro Sakita, Hiroyo Kondo, Miho Kanazashi, Masayuki Tanaka, Minoru Tanaka, Aiji Ohtuka, Ryusuke Momota, Hidemi Fujino

FASEB JOURNAL 28 ( 1 ) 2014.4

致死となるマルチプレキシン(XV/XVIII型コラーゲン)変異ショウジョウバエの解析

百田龍輔, 楢崎正博, 小見山高明, 内藤一郎, 二宮善文, 大塚愛二

日本結合組織学会学術大会・マトリックス研究会大会合同学術集会プログラム・抄録集 45回・60回 86 - 86 2013.6

Drosophila type XV/XVIII collagen mutants manifest integrin mediated mitochondrial dysfunction, which is improved by cyclosporin A and losartan Reviewed International journal

Ryusuke Momota, Masahiro Narasaki, Takaaki Komiyama, Ichiro Naito, Yoshifumi Ninomiya, Aiji Ohtsuka

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 45 ( 5 ) 1003 - 1011 2013.5

Language：English Publishing type：Research paper (scientific journal) Publisher：PERGAMON-ELSEVIER SCIENCE LTD

Vertebrate collagen types XV and XVIII are broadly distributed basement membrane components, classified into a structurally distinct subgroup called "multiplexin collagens". Mutations in mammalian multiplexins are identified in some degenerative diseases such as Knobloch syndrome 1 (KNO1) or skeletal/cardiac myopathies, however, these progressive properties have not been elucidated. Here we investigated Drosophila mutants of Multiplexin (Mp), the only orthologue of vertebrate collagen types XV and XVIII, to understand the pathogenesis of multiplexin-related diseases. The mp mutants exhibited morphological changes in cardiomyocytes and progressive dysfunction of the skeletal muscles, reminiscent phenotypes observed in Col15a1-null mice. Ultrastructural analysis revealed morphologically altered mitochondria in mutants' indirect flight muscles (IFMs), resulting in severely attenuated ATP production and enhanced reactive oxygen species (ROS) production. In addition, mutants' IFMs exhibited diminished PPS integrin clustering and abolished focal adhesion kinase (FAK) phosphorylation. Furthermore, mutants' defective IFMs are improved by the administrations of cyclosporin A, an inhibitor against mitochondrial permeability transition pore (mPTP) opening or losartan, an angiotensin II type 1 receptor (AT1R) blocker. Thus, our results suggest that Mp modulates mPTP opening and AT1R activity through its binding to integrin and that lack of Mp causes unregulated mPTP opening and AT1R activity, leading to mitochondrial dysfunctions. Hence, our results provide new insights towards the roles of multiplexin collagens in mitochondrial homeostasis and may serve as pharmacological evidences for the potential use of cyclosporin A or losartan for the therapeutic strategies. (C) 2013 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.biocel.2013.02.001

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Architecture of the subendothelial elastic fibers of small blood vessels and variations in vascular type and size Reviewed

Akira Shinaoka, Ryusuke Momota, Eri Shiratsuchi, Mitsuko Kosaka, Kanae Kumagishi, Ryuichi Nakahara, Ichiro Naito, Aiji Ohtsuka

Microscopy and Microanalysis 19 ( 2 ) 406 - 414 2013.4

Language：English Publishing type：Research paper (scientific journal)

Most blood vessels contain elastin that provides the vessels with the resilience and flexibility necessary to control hemodynamics. Pathophysiological hemodynamic changes affect the remodeling of elastic components, but little is known about their structural properties. The present study was designed to elucidate, in detail, the three-dimensional (3D) architecture of delicate elastic fibers in small vessels, and to reveal their architectural pattern in a rat model. The fine vascular elastic components were observed by a newly developed scanning electron microscopy technique using a formic acid digestion with vascular casts. This method successfully visualized the 3D architecture of elastic fibers in small blood vessels, even arterioles and venules. The subendothelial elastic fibers in such small vessels assemble into a sheet of meshwork running longitudinally, while larger vessels have a higher density of mesh and thicker mesh fibers. The quantitative analysis revealed that arterioles had a wider range of mesh density than venules
the ratio of density to vessel size was higher than that in venules. The new method was useful for evaluating the subendothelial elastic fibers of small vessels and for demonstrating differences in the architecture of different types of vessels. © Microscopy Society of America 2013.

DOI： 10.1017/S1431927612014341

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XV/XVIII型コラーゲンの新たな生物学的機能の探索

百田龍輔, 内藤一郎, 二宮善文, 大塚愛二

解剖学雑誌 87 ( 2 ) 44 - 44 2012.6

Abnormalities in the Fiber Composition and Capillary Architecture in the Soleus Muscle of Type 2 Diabetic Goto-Kakizaki Rats Reviewed

Shinichiro Murakami, Naoto Fujita, Hiroyo Kondo, Isao Takeda, Ryusuke Momota, Aiji Ohtsuka, Hidemi Fujino

SCIENTIFIC WORLD JOURNAL 2012 680189 2012

Language：English Publishing type：Research paper (scientific journal) Publisher：HINDAWI PUBLISHING CORPORATION

Type 2 diabetes mellitus is linked to impaired skeletal muscle glucose uptake and storage. This study aimed to investigate the fiber type distributions and the three-dimensional (3D) architecture of the capillary network in the skeletal muscles of type 2 diabetic rats. Muscle fiber type transformation, succinate dehydrogenase (SDH) activity, capillary density, and 3D architecture of the capillary network in the soleus muscle were determined in 36-week-old Goto-Kakizaki (GK) rats as an animal model of nonobese type 2 diabetes and age-matched Wistar (Cont) rats. Although the soleus muscle of Cont rats comprised both type I and type IIA fibers, the soleus muscle of GK rats had only type I fibers. In addition, total SDH activity in the soleus muscle of GK rats was significantly lower than that in Cont rats because GK rats had no high-SDH activity type IIA fiber in the soleus muscle. Furthermore, the capillary diameter, capillary tortuosity, and microvessel volume in GK rats were significantly lower than those in Cont rats. These results indicate that non-obese diabetic GK rats have muscle fiber type transformation, low SDH activity, and reduced skeletal muscle capillary content, which may be related to the impaired glucose metabolism characteristic of type 2 diabetes.

DOI： 10.1100/2012/680189

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Drosophila type XV/XVIII collagen, Mp, is involved in Wingless distribution Reviewed International journal

Ryusuke Momota, Ichiro Naito, Yoshifumi Ninomiya, Aiji Ohtsuka

MATRIX BIOLOGY 30 ( 4 ) 258 - 266 2011.5

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

Multiplexin (Mp) is the Drosophila orthologue of vertebrate collagens XV and XVIII. Like them, Mp is widely distributed in the basement membranes of the developing embryos, including those of neuroblasts in the central and peripheral nervous systems, visceral muscles of the gut, and contractile cardioblasts. Here we report the identification of mutant larvae bearing piguBac transposon insertions that exhibit decrease Mp production associated with abdominal cuticular and wing margin defects, malformation of sensory organs and impaired sensitivity to physical stimuli. Additional findings include the abnormal ultrastructure of fatbody associated with abnormal collagen IV deposition, and reduced Wingless deposition. Collectively, these findings are consistent with the notion that Mp is required for the proper formation and/or maintenance of basement membrane, and that Mp may be involved in establishing the Wingless signaling gradients in the Drosophila embryo. (C) 2011 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.matbio.2011.03.008

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微小血管内皮下弾性線維構造とその血行動態との関係

品岡玲, 百田龍輔, 小阪美津子, 内藤一郎, 中原龍一, 大塚愛二

日本結合組織学会学術大会・マトリックス研究会大会合同学術集会プログラム・抄録集 43回・58回 105 - 105 2011.5