Updated on 2022/07/26

写真a

 
MOMINOKI Katsumi
 
Organization
Advanced Science Research Center Professor
Position
Professor
External link

Degree

  • 博士(獣医学)

Research Interests

  • Neuroanatomy

  • Comparative Biochemistry

  • Experimental Zoology

Research Areas

  • Life Science / Laboratory animal science

Education

  • Hokkaido University   大学院獣医学研究科   形態機能学専攻

    1994.4 - 1998.3

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    Country: Japan

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  • Hokkaido University   獣医学部   獣医学科

    1990.4 - 1994.3

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    Country: Japan

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  • Hokkaido University    

    1988.4 - 1990.3

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Research History

  • Okayama University   Department of Animal Resources, Advanced Science Research Center   Professor

    2015.4

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  • Okayama University   Department of Animal Resources, Advanced Science Research Center   Associate Professor

    2007.4 - 2015.3

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  • Okayama University   Department of Animal Resources, Advanced Science Research Center   Associate Professor (as old post name)

    2007.1 - 2007.3

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  • Ehime University   Department of Biological Resources, Integrated Center for Sciences   Research Assistant

    2003.4 - 2006.12

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  • Ehime University   Laboratory Animal Center, School of Medicine   Research Assistant

    2000.4 - 2003.3

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  • Ehime University   Department of 1st Anatomy, School of Medicine, Faculty of Medicine   Research Assistant

    1998.4 - 2000.3

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  • Hokkaido University   Graduate School of Veterinary Medicine

    1996.4 - 1998.3

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Professional Memberships

  • 日本実験動物技術者協会

    2011.4

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  • 岡山実験動物研究会

    2009.4

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  • 九州実験動物研究会

    2008.11

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  • The Japanese Association for Laboratory Animal Medicine

    2000.9

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  • 日本解剖学会

    1998.4 - 2020.12

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  • 日本獣医学会

    1992.9

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  • 日本実験動物医学会

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Committee Memberships

  • 岡山実験動物研究会   会長  

    2019.1   

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  • 国立大学法人動物実験施設協議会   中型動物委員長  

    2018.7   

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    Committee type:Other

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  • 日本実験動物学会   評議員  

    2016.8   

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  • 日本獣医学会   評議員  

    2015.10   

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  • 岡山実験動物研究会   理事  

    2015.4 - 2018.12   

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  • 九州実験動物研究会   評議員  

    2008.11   

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Papers

  • 実験動物の安楽死の課題 安楽死処置におけるセコバルビタールの有用性 Invited

    赤木佐千子, 平山晴子, 樅木勝巳

    Labio 21   ( 81 )   2020

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  • Risk assessment for hepatitis E virus infection from domestic pigs introduced into an experimental animal facility in a medical school. Reviewed

    Hirohito Ogawa, Haruko Hirayama, Satsuki Tanaka, Norio Yata, Hikaru Namba, Nobuko Yamashita, Kenzo Yonemitsu, Ken Maeda, Katsumi Mominoki, Masao Yamada

    The Journal of veterinary medical science   81 ( 8 )   1191 - 1196   2019.8

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    Hepatitis E virus (HEV) is known to cause zoonotic infections from pigs, wild boars and deer. Domestic pigs have been used as an experimental animal model in medical research and training; however, the risks of HEV infection from pigs during animal experiments are largely unknown. Here, we retrospectively investigated the seroprevalence and detection rates of viral RNA in 73 domestic pigs (average 34.5 kg) introduced into an animal experimental facility in a medical school during 2012-2016. We detected anti-HEV immunoglobulin G antibodies in 24 of 73 plasma samples (32.9%), though none of the samples were positive for viral RNA. Plasma samples of 18 pigs were sequentially monitored and were classified into four patterns: sustained positive (5 pigs), sustained negative (5 pigs), conversion to positive (6 pigs) and conversion to negative (2 pigs). HEV genomes were detected in 2 of 4 liver samples from pigs that were transported from the same farm during 2016-2017. Two viral sequences of the overlapping open reading frame (ORF) 2/3 region (97 bp) were identical and phylogenetically fell into genotype 3. A 459-bp length of the ORF2 region of an amplified fragment from a pig transported in 2017 was clustered with the wbJYG1 isolate (subgenotype 3b) with 91.5% (420/459 bp) nucleotide identity. Based on our results, we suggest that domestic pigs introduced into animal facilities carry a potential risk of HEV infection to researchers, trainees and facility staff. Continuous surveillance and precautions are important to prevent HEV infection in animal facilities.

    DOI: 10.1292/jvms.19-0086

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  • 岡山大学自然生命科学研究支援センターに搬入される家畜ブタのE型肝炎ウイルス感染状況について

    小川寛人, 平山晴子, 田中爽暉, 矢田範夫, 難波ひかる, 山下信子, 米満研三, 前田健, 樅木勝巳, 山田雅夫

    岡山実験動物研究会報   ( 35 )   2019

  • ラットの経時的採血におけるストレッチハンドリングのストレス軽減効果の検討

    平山晴子, 中村綾花, 樅木勝巳

    岡山実験動物研究会報   ( 35 )   2019

  • Effects of Endoprosthesis Head Material on Acetabular Cartilage Metabolism: An Animal Study Using Crossbred Pigs. Reviewed

    Shuhei Matsui, Tokifumi Majima, Katsumi Mominoki, Haruko Hirayama, Yasushi Oshima, Kenji Takahashi, Shinro Takai

    Journal of Nippon Medical School = Nippon Ika Daigaku zasshi   85 ( 6 )   309 - 314   2018

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    BACKGROUND: Hip endoprosthesis is one option for the treatment of displaced femoral neck fractures and avascular necrosis of the femoral head. Few reports are available describing acetabular cartilage metabolism after endoprosthesis surgery of the hip. The purpose of this study was to compare the biological effects on cartilage between cobalt-chrome (Co-Cr) and alumina ceramic heads wherein the cartilage articulates directly. METHODS: We used the acetabular cartilage from six hips of three immature crossbred pigs to examine the effects on cytokines, the amount of hyaluronic acid (HA), and cartilage mRNA expression of ceramic head and Co-Cr head endoprosthesis. Mechanical loading of materials of Co-Cr and ceramic heads was performed on the acetabular cartilage in culture media as an organ culture model. Thereafter, protein levels of cytokines (MMP-1, 3, TNF-alpha (α), Interleukin (IL)-1 alpha (α), and IL-1 beta (β)) and the amount of HA were measured from the culture media. Cartilage RNA extraction was performed, and quantitative reverse transcriptase-polymerase chain reaction was performed with primer sets for type I, II, and III collagens; aggrecan; MMP-1, 3, 13; TNF-α; and IL-1 α, IL-1 β. RESULTS: Protein level of IL-1 β and amount of HA in the Co-Cr group were significantly higher than those of the Ceramic group. Type II collagen mRNA expression in the Ceramic group was significantly higher than in the Co-Cr group. IL-1 β mRNA expression was significantly higher in the Co-Cr group than in the Ceramic group. CONCLUSIONS: The present study showed that ceramic bipolar produces smaller adverse effects on cartilage cells compared to Co-Cr bipolar. These results could have significant implications for implant usage not only in hip joints, but also in other joints, including the shoulder, talus and radial head.

    DOI: 10.1272/jnms.JNMS.2018_85-50

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  • 動物実験に関する相互検証プログラム実施に向けて-岡山大学における実施前準備について-

    樅木勝巳

    実験動物と環境   ( 51 )   2018

  • 歯周病原細菌によるヒトと伴侶動物イヌとの人獣共通感染症検査の研究

    田井真砂子, 伊東孝, 平山晴子, 矢田範夫, 小川寛人, 田村和也, 伊東有希, 大久保圭祐, 伊東昌洋, 中村心, 岡本憲太郎, 平井公人, 山城圭介, 大森一弘, 山本直史, 樅木勝巳, 高柴正悟

    日本歯周病学会会誌(Web)   60   2018

  • Confirmation test of education and training by e-learning system, for animal experiment researchers

    矢田範夫, 上藤千佳, 平山晴子, 樅木勝巳

    岡山実験動物研究会報   ( 32 )   2016

  • Conception of establishing a centralized animal facility for the institutional management to use the laboratory animals for research and education in Tsushima campus, Okayama University

    樅木勝巳

    岡山実験動物研究会報   ( 32 )   2016

  • バーコードを利用した実験動物飼育管理データシステムの構築

    上藤千佳, 矢田範夫, 上山和貴, 荒川雅行, 藤井匡寛, 平山晴子, 樅木勝巳

    岡山実験動物研究会報   ( 31 )   2015

  • [Effect of ghrelin on colonic motility].

    Haruko Hirayama, Katsumi Mominoki, Takahiko Shiina, Yasutake Shimizu

    Nihon yakurigaku zasshi. Folia pharmacologica Japonica   143 ( 6 )   270 - 4   2014.6

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  • 脊髄を介するグレリンの大腸運動促進作用

    平山晴子, 樅木勝巳, 椎名貴彦, 志水泰武

    岡山実験動物研究会報   ( 29 )   2013

  • Sensory tract abnormality in the chick model of spina bifida. Reviewed International journal

    Ryusuke Tsujimura, Katsumi Mominoki, Masae Kinutani, Tetsuya Shimokawa, Takuya Doihara, Hiroaki Nabeka, Hiroyuki Wakisaka, Naoto Kobayashi, Seiji Matsuda

    Neuroscience research   71 ( 1 )   85 - 91   2011.9

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    Spina bifida aperta (SBA) is an open neural tube defect that occurs during the embryonic period. We created SBA chicks by incising the roof plate of the neural tube in the embryo. The area of the dorsal funiculus was smaller in the SBA chicks than in the normal controls. Additionally, the SBA group had fewer nerve fibres in the dorsal funiculus than the normal controls. The pathway of the ascending sensory nerves was revealed by tracing the degenerated nerve fibres using osmification. We cut the sciatic nerve (L5) of the control and SBA chicks at the central end of the dorsal root ganglion 1 day after hatching and fixed the tissue 3 days later. Degenerated sensory nerve fibres were observed in the ipsilateral dorsal funiculus in the control chicks. In contrast, degenerated sensory nerve fibres were observed in the ipsilateral and contralateral dorsal, ventral and lateral funiculi of the spinal cord in the SBA chicks. Consequently, fewer sensory nerve fibres ascended to the thoracic dorsal funiculus in the SBA chicks than in the normal controls. This is the first report of abnormal changes in the ascending sensory nerve fibres in SBA.

    DOI: 10.1016/j.neures.2011.05.017

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  • Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51. Reviewed International journal

    Kazuhiko Ochiai, Yasunaga Yoshikawa, Kumiko Yoshimatsu, Toshina Oonuma, Yukiko Tomioka, Eichi Takeda, Jiro Arikawa, Katsumi Mominoki, Toshinori Omi, Kazuyoshi Hashizume, Masami Morimatsu

    FEBS letters   585 ( 12 )   1771 - 7   2011.6

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    The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via eight BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51.
    Structured summary of protein interactions:
    cRAD51 physically interacts with cRAD51 by two hybrid (View interaction)
    fBRC4 physically interacts with cRAD51 by two hybrid (View interaction)
    cBRC4 physically interacts with cRAD51 by two hybrid (View interaction)
    hBRC4 physically interacts with hBRC4 and hRAD51 by competition binding (View Interaction 1, 2)
    hBRC4 physically interacts with cRAD51 by two hybrid (View interaction)
    hBRC4 binds to hRAD51 by enzyme linked immunosorbent assay (View interaction)
    (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.febslet.2011.05.027

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  • Developmental delay in islet-1-positive motor neurons in chick spina bifida. Reviewed

    Min Wang, Katsumi Mominoki, Masae Kinutani, Zhong Wang, Naoto Kobayashi, Tetsuya Shimokawa, Hiroaki Nabeka, Takashi Fujiwara, Seiji Matsuda

    The Journal of veterinary medical science   73 ( 4 )   447 - 52   2011.4

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    Spina bifida aperta (SBA) is a congenital malformation of the spinal cord with complications such as spinal ataxia and bowel and bladder dysfunction. We have developed a chick model with surgery-induced SBA that shows spinal ataxia after hatching. In the present study, motor neurons in the early stages in chicks with and without SBA were observed by immunohistochemical staining with a monoclonal antibody against Islet-1, a motor neuron marker. Delay in migration and maturation of motor neurons was observed in SBA. Although the final numbers of Islet-1-positive neurons in these two groups were not different, a defect in the production and elimination of excess motor neurons in the early developmental stages in the SBA group may be involved in the pathological mechanism of the motor complications of this disease.

    DOI: 10.1292/jvms.10-0385

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  • Chronological changes in Islet-1-positive neurons in a chick model of spina bifida aperta Reviewed

    Min WANG, Katsumi MOMINOKI, Masae KINUTANI, Zhong WANG, Naoto KOBAYASHI, Tetsuya SHIMOKAWA, Hiroaki NABEKA, Takashi FUJIWARA, Seiji MATSUDA

    J Vet Med Sci   73 ( 4 )   447 - 452   2011

  • Targeting KRAS mutation-bearing lung cancer in vivo by pulmonary surfactant-adenovirus-mediated gene transfer. Reviewed International journal

    Takuya Fukazawa, Yutaka Maeda, Junji Matsuoka, Toshiro Ono, Katsumi Mominoki, Tomoki Yamatsuji, Kaoru Shigemitsu, Ichiro Morita, Ichiro Murakami, Hirotoshi Tanaka, Mary L Durbin, Yoshio Naomoto

    Anticancer research   30 ( 12 )   4925 - 35   2010.12

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    Pulmonary surfactant has been used as a carrier to deliver a therapeutic virus to dysfunctional lung cells that reside within an intricate lung structure. To investigate whether pulmonary surfactant enhances the efficacy of intratracheal instillation of a therapeutic virus to target KRAS mutation-bearing lung cancer in vivo, we developed a recombinant adenovirus that induces cell death only in lung cancer cells and injected the adenovirus into a mouse model of KRAS mutation-positive lung cancer intratracheally with and without surfactant. A therapeutic adenovirus that induces cell death only in lung cancer cells was constructed by combining a cancer-specific human telomerase reverse transcriptase (hTERT) promoter fused to CCAATIenhancerbinding protein alpha (CEBPa) with a modified lung-specific Clara cell-specific 10-κDa protein (CClO) promoter fused to cytotoxic adenovirus type 5 early region 1A (E1A). CEBPa is induced only in cancer cells and activates the CC10 promoter, which in turn induces cytotoxic E1A, and causes cell death only in lung cancer cells in vitro. This adenovirus was intratracheally administered to the model mice (CCSP-rtTA/Tet-op-K-Ras4bG12D bitransgenic mice) in the presence and absence of pulmonary surfactant. Intratracheally administered therapeutic adenovirus with pulmonary surfactant spread to airways, as well as to the alveolar region of the lung, and caused a reduction of lung tumors developed. The therapeutic adenovirus without pulmonary surfactant spread only to airways and was tenfold less effective in tumor reduction. Here, we demonstrate that pulmonary surfactant is an efficient tool to intratracheally deliver a therapeutic virus to treat KRAS mutation-positive lung cancer in vivo.

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  • 岡山大学自然生命科学研究支援センター動物資源部門鹿田施設の管理・運営の実際-動物実験施設の管理・運営改革2年間を振り返って-

    樅木勝巳

    岡山実験動物研究会報   ( 25 )   2009

  • 組織および癌特異的目的遺伝子発現システムによる肺腺癌治療への応用および抗癌剤併用効果の検討

    深澤拓也, 松岡順治, 猶本良夫, 中井徹, 小野俊朗, 樅木勝巳, 田中廣壽, 前田豊, DURBIN Mary, 田中紀章

    日本外科学会雑誌   110   2009

  • Expression patterns in alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus after facial nerve transection. Reviewed

    Chen Jie, Saito Shouichiro, Kobayashi Naoto, Sato Kohji, Terashita Takehiro, Shimokawa Tetsuya, Mominoki Katsumi, Miyawaki Kyojy, Sano Akira, Matsuda Seiji

    Neuroscience research   60 ( 1 )   82   2008.1

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    Prosaposin acts as a neurotrophic factor, in addition to its role as the precursor protein for saposins A, B, C, and D, which are activators for specific sphingolipid hydrolases in lysosomes. In rats, the prosaposin gene generates two alternative splicing forms of mRNA: Pro+9 containing a 9-base insertion and Pro+0 without. The expression of these mRNAs changes after brain injury. We examinedthe expression patterns of the alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus for 52 days following facial nerve transection. Pro+0 mRNA increased within 3 days of transection, peaked after 5-10 days, and remained significantly elevated for 21 days. In contrast, the expression of Pro+9 mRNA was constant throughout the regenerative period. Prosaposin mRNA expression increased not only in facial motoneurons, but also in microglia during facial nerve regeneration. Our findings indicate that the saposin B domain of prosaposin, which is thedomain affected by alternative splicing, plays an important role in both neurons andmicroglia during neuroregeneration.

    DOI: 10.1016/j.neures.2007.09.010

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  • Expression patterns in alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus after facial nerve transection. Reviewed International journal

    Jie Chen, Shouichiro Saito, Naoto Kobayashi, Kohji Sato, Takehiro Terashita, Tetsuya Shimokawa, Katsumi Mominoki, Kyojy Miyawaki, Akira Sano, Seiji Matsuda

    Neuroscience research   60 ( 1 )   82 - 94   2008.1

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    Prosaposin acts as a neurotrophic factor, in addition to its role as the precursor protein for saposins A, B, C, and D, which are activators for specific sphingolipid hydrolases in lysosomes. In rats, the prosaposin gene generates two alternative splicing forms of mRNA: Pro + 9 containing a 9-base insertion and Pro + 0 without. The expression of these mRNAs changes after brain injury. We examined the expression patterns of the alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus for 52 days following facial nerve transection. Pro + 0 mRNA increased within 3 days of transection, peaked after 5-10 days, and remained significantly elevated for 21 days. In contrast, the expression of Pro + 9 mRNA was constant throughout the regenerative period. Prosaposin mRNA expression increased not only in facial motoneurons, but also in microglia during facial nerve regeneration. Our findings indicate that the saposin B domain of prosaposin, which is the domain affected by alternative splicing, plays an important role in both neurons and microglia during neuroregeneration. © 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society.

    DOI: 10.1016/j.neures.2007.09.010

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  • Myasthenia gravis experimentally induced with muscle-specific kinase. Reviewed International journal

    Kazuhiro Shigemoto, Sachiho Kubo, Chen Jie, Naohito Hato, Yasuhito Abe, Norifumi Ueda, Naoto Kobayashi, Kenji Kameda, Katsumi Mominoki, Atsuo Miyazawa, Akihito Ishigami, Seiji Matsuda, Naoki Maruyama

    Annals of the New York Academy of Sciences   1132   93 - 8   2008

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    Here we present the first evidence that muscle-specific kinase (MuSK) antigen can cause myasthenia in animals. MuSK is expressed at the postsynaptic membranes of neuromuscular junctions (NMJ) and forms complexes with acetylcholine receptors (AChR) and rapsyn. MuSK is activated by agrin, which is released from motoneurons, and induces AChR clustering and subsequent formation of NMJ in embryos. Notably, autoantibodies against MuSK were found in a proportion of patients with generalized myasthenia gravis (MG) but without the characteristic AChR autoantibodies. However, MuSK autoantibodies had no known pathogenic potential, and animals immunized with purified MuSK proteins did not develop MG in former studies. In contrast, we have now injected rabbits with MuSK ectodomain protein in vivo and evoked a MG-like muscle weakness with a reduction of AChR clustering at the NMJ. Our results showed that MuSK is required for maintenance of synapses and that interference with that function by MuSK antibodies causes myasthenic weakness. In vitro, AChR clustering in myotubes is induced by agrin and agrin-independent inducers, which do not activate MuSK. Neither the receptor nor the activation mechanisms of AChR clustering induced by agrin-independent inducers has been identified with certainty, but MuSK autoantibodies in myasthenic animals inhibited both agrin and agrin-independent AChR clustering. MuSK plays multiple roles in pre-patterning of the postsynaptic membrane before innervation and formation of NMJ in embryos. Some of these mechanisms may also participate in the maintenance of mature NMJ. This model system would provide new knowledge about the molecular pathogenesis of MG and MuSK functions in mature NMJ.

    DOI: 10.1196/annals.1405.002

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  • 線維芽細胞をめぐる最新の知見 組織常在性線維芽細胞は新生血管の内皮細胞に分化する

    藤原隆, 昆和典, 樅木勝巳, 大沼俊名

    顕微鏡   43 ( 2 )   2008

  • 二分脊椎モデルニワトリにおける運動障害は運動ニューロンの発生異常に拠るのだろうか

    渡部聰枝, 松丸美香, 樅木勝巳, 松田正司, 絹谷政江

    形態・機能   6 ( 1 )   2007

  • Induction of myasthenia by immunization against muscle-specific kinase. Reviewed International journal

    Kazuhiro Shigemoto, Sachiho Kubo, Naoki Maruyama, Naohito Hato, Hiroyuki Yamada, Chen Jie, Naoto Kobayashi, Katsumi Mominoki, Yasuhito Abe, Norifumi Ueda, Seiji Matsuda

    The Journal of clinical investigation   116 ( 4 )   1016 - 24   2006.4

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    Muscle-specific kinase (MuSK) is critical for the synaptic clustering of nicotinic acetylcholine receptors (AChRs) and plays multiple roles in the organization and maintenance of neuromuscular junctions (NMJs). MuSK is activated by agrin, which is released from motoneurons, and induces AChR clustering at the postsynaptic membrane. Although autoantibodies against the ectodomain of MuSK have been found in a proportion of patients with generalized myasthenia gravis (MG), it is unclear whether MuSK autoantibodies are the causative agent of generalized MG. In the present study, rabbits immunized with MuSK ectodomain protein manifested MG-like muscle weakness with a reduction of AChR clustering at the NMJs. The autoantibodies activated MuSK and blocked AChR clustering induced by agrin or by mediators that do not activate MuSK. Thus MuSK autoantibodies rigorously inhibit AChR clustering mediated by multiple pathways, an outcome that broadens our general comprehension of the pathogenesis of MG.

    DOI: 10.1172/JCI21545

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  • Leg dysfunctions in a hatched chick model of spina bifida aperta. International journal

    Katsumi Mominoki, Masae Kinutani, Hiroyuki Wakisaka, Shouichirou Saito, Naoto Kobayashi, Takashi Fujiwara, Seiji Matsuda

    Experimental neurology   197 ( 1 )   133 - 42   2006.1

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    We created chicks with spina bifida aperta (SBA) by incising the roof plate of the neural tube of embryos at Hamburger and Hamilton stage 18 or 19. Incision over the length of three somites caused spina bifida occulta (SBO)-like malformation in 47% of the hatchlings. Incision over the length of five and seven somites caused SBA-like malformation in 100% of the hatchlings. The SBO chicks exhibited no symptoms, whereas the SBA chicks exhibited paralysis of a leg muscle and imbalance between an agonist and an antagonist leg muscles. Lesions in these SBA chicks were located in the spinal segments that give rise to motor neurons that innervated the dysfunctional muscles. Histological analysis revealed that there were fewer small spinal neurons (interneurons) at the site of the lesion in SBA chicks than in the normal chicks and that there was no such difference in the number of the large spinal neurons (motor neurons). Leg dysfunctions in this model of SBA may be attributable to the smaller number of interneurons in the spinal segments that contain motor neurons that innervate the dysfunctional muscle. This model may facilitate studies of the pathological mechanisms that lead to leg dysfunctions in SBA chicks. © 2005 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.expneurol.2005.09.001

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  • Elevated plasma concentrations of haptoglobin in European brown bears during hibernation International journal

    K Mominoki, M Morimatsu, M Kaijalainen, E Hohtola, R Hissa, M Saito

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY   142 ( 4 )   472 - 477   2005.12

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    Haptoglobin (Hp), a hemoglobin-binding protein, is known as an acute phase protein and increases during the acute phase of inflammation in most mammals. We reported previously in brown bears that the mean Hp concentrations were higher in blood samples obtained in winter than those in spring. To examine a possible relation of the seasonal variations of Hp to hibernation, in the present study, we measured the plasma concentrations of Hp as well as some other acute phase proteins (alpha(2)-macroglobulin, alpha(1)-antitrypsin, C-reactive protein) in 6 European brown bears (Ursus arctos), from which blood samples were obtained at 5-6 different months of year including February, the time of hibernation. The Hp concentrations showed clear seasonal variations, being highest in February. The alpha(2)-macroglobulin concentrations also showed a similar but much smaller rise in February, but those of alpha(1)-antitrypsin and C-reactive protein did not show any seasonal variations. Our results suggest that the seasonal variation of plasma Hp concentration in brown bears is associated with a hibernation-specific mechanism more than that of acute phase response. (C) 2005 Elsevier Inc. All rights reserved.

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  • Phylogenetic investigation of Dogiel's pericellular nests and Cajal's initial glomeruli in the dorsal root ganglion. International journal

    Seiji Matsuda, Naoto Kobayashi, Takehiro Terashita, Tetsuya Shimokawa, Kazuhiro Shigemoto, Katsumi Mominoki, Hiroyuki Wakisaka, Shouichiro Saito, Kyoujy Miyawaki, Kyoko Saito, Fumiki Kushihata, Jie Chen, Shuang-Yan Gao, Chun-Yu Li, Min Wang, Takashi Fujiwara

    The Journal of comparative neurology   491 ( 3 )   234 - 45   2005.10

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    Cajal's initial glomeruli (IG) and Dogiel's pericellular nests (PCNs) were first described from methylene blue preparations of healthy animal tissues around the beginning of the last century. Since that time, although many reports have been published concerning these structures, few have focused on their development and phylogeny in healthy animals. The aim of this study was to examine the phylogenetic development of the sensory neurons in Cajal's IG (also called axonal glomeruli) and Dogiel's PCNs in the dorsal root ganglion (DRG) of the healthy adult frog, chick, rat, and rabbit. The three-dimensional architecture of the neurons was observed in ganglia by scanning electron microscopy after removal of the connective tissue. The neurons in the DRG of fish are known to be bipolar, but DRG neurons in the species examined here were found to be pseudounipolar, with single stem processes. The proportion of neurons having IG or PCNs increased with increasing phylogenetic complexity in the species examined here. Cajal's initial glomeruli, the convolution of the stem process near the parent cell body: In frogs, the ganglia were small and the neuronal stem processes were very short and straight. In chicks, the stem processes were longer; sometimes very long, tortuous processes were observed. However, no neurons with typical IG were observed in either species. Typical IG were observed in rats and rabbits; their occurrence was much more frequent in rabbits. Pseudounipolarization, i.e., the transition from bipolar to pseudounipolar neurons, is thought to save space, limit the length of neuronal processes, and reduce conduction time. However, an explanation of the evolutionary advantage of the IG, which is formed by the excessive prolongation of the stem process, remains elusive. The cytological and electrophysiological importance of IG has been discussed. Dogiel's pericellular nests (PCNs), which resemble balls of yarn made of thin unmyelinated nerve fibers around DRG neurons, have been observed in the DRG of rats and rabbits, but not in frogs or chicks. This interesting structure shows not only ontogenetic development in healthy animals but also phylogenetic development among species. The nerve fibers in the PCNs were less than 1.2 mu m in diameter and had some varicosities. An immunohistochemical study using anti-tyrosine hydroxylase (TH) antibody revealed that some PCNs contain TH-positive nerve fibers and varicosities. Such TH-positive PCNs disappear after sympathectomy. These results suggest that the PCNs are made up of autonomic nerve fibers.

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  • Seasonal changes in serum leptin of the feral raccoon (Procyon lotor) determined by canine-leptin-specific ELISA. International journal

    Haruki Shibata, Rie Akahane, Tsutomu Honjoh, Makoto Asano, Katsumi Mominoki, Kei Fujii, Masatsugu Suzuki, Noriyuki Ohtaishi, Katsumi Ishioka, Mohamed Ahmed, Mohamed Soliman, Kazuhiro Kimura, Masayuki Saito

    Journal of experimental zoology. Part A, Comparative experimental biology   303 ( 7 )   527 - 33   2005.7

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    Several reports have been published on blood leptin concentrations in feral animals, including members of the Carnivora, using a commercially available multi-species radioimmunoassay (RIA) kit with anti-human leptin antibody. However, we observed weak immunoreactivity between recombinant canine leptin and anti-human leptin antibody, suggesting a limitation in the applicability of the RIA kit for leptin assays in Carnivora species. We tested the applicability of RIA and sandwich enzyme-linked immunosorbent assay (ELISA) with anti-canine leptin antibody to assay blood leptin in the dog (Canis familiaris) and the raccoon (Procyon lotor). When RIA was used for recombinant canine leptin and dog sera, values were much lower than those determined by ELISA at higher concentrations (> 10 ng/ml), while rather higher at lower concentrations (< 2ng/ml). A similar discrepancy between the two methods was found for serum leptin concentrations in raccoons. Clear seasonal variations were observed by ELISA, but not by RIA, with high values in autumn (3.46 +/- 0.45ng/ml) and low values in spring and summer (0.71 +/- 0.07ng/ml). Serum leptin concentrations in raccoons correlated positively with their body weight (r = 0.753) and body mass index (r = 0.755), corroborating our previous findings of a strong positive correlation between serum leptin concentrations and body fat content in dogs. Thus, the canine leptin ELISA is useful for assays of dog and raccoon leptin, and blood leptin is a good marker of nutritional condition in the species of Carnivora assayed in this study. (c) 2005 Wiley-Liss, Inc.

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  • Changes in expression of prosaposin in the rat facial nerve nucleus after facial nerve transection International journal

    Kana Unuma, Jie Chen, Shouichiro Saito, Naoto Kobayashi, Kohji Sato, Kyoko Saito, Hiroyuki Wakisaka, Katsumi Mominoki, Akira Sano, Seiji Matsuda

    Neuroscience Research   52 ( 3 )   220 - 227   2005.7

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    Prosaposin is the precursor of saposins A, B, C and D, which are activators of sphingolipid hydrolases. In addition, unprocessed prosaposin functions as a neurotrophic factor in the central and peripheral nervous systems by acting to prevent neuronal apoptosis, to elongate neurites and to facilitate myelination. In this study, the expression pattern of prosaposin in the facial nerve nucleus after facial nerve transection was examined by immunohistochemistry and in situ hybridization. Prosaposin immunoreactivity in the neurons on the operated side facial nerve nucleus showed a biphasic pattern: it was significantly increased on day 3 after transection, decreased dramatically on day 7, started to increase gradually on day 14 and reached another peak on day 21 after transection. Significant increases in the levels of prosaposin mRNA were identified in the neurons on the operated side, suggesting that prosaposin was synthesized vigorously by the neurons themselves in the case of facial nerve transection. The diverse changes in prosaposin immunoreactivity during the process of facial nerve regeneration may reflect the diverse neurotrophic activities of prosaposin in facial motoneurons. © 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

    DOI: 10.1016/j.neures.2005.03.009

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  • Transient increase of TUNEL-positive cells on postnatal day 20 in the developing rat olfactory bulb International journal

    K Saito, S Saito, K Taniguchi, N Kobayashi, T Terashita, T Shimokawa, K Mominoki, K Miyawaki, J Chen, SY Gao, CY Li, S Matsuda

    NEUROSCIENCE RESEARCH   50 ( 2 )   219 - 225   2004.10

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    In the developing central nervous system, apoptosis plays an important role in the normal organization of the neuronal circuit. The timing of neurogenesis, proliferation, and migration of the neurons in the developing olfactory bulb (OB) is well studied; however, the involvement of apoptosis in this process is not fully understood. In this study, we examined the changes in the distribution and the number of apoptotic cells in the rat OB during embryonic and postnatal periods, by using terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL) staining. Although the number of TUNEL-positive cells was relatively small during the embryonic period, it gradually increased after birth, and peaked on postnatal day 20 with statistical significance, especially in the granule cell layer of the main OB. This transient increase of TUNEL-positive cells on postnatal day 20 may be involved in a critical event during maturation of the OB. (C) 2004 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

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  • Prognostic significance of Bcl-xL in human hepatocellular carcinoma. International journal

    Jota Watanabe, Fumiki Kushihata, Kazuo Honda, Atsuro Sugita, Norihiko Tateishi, Katsumi Mominoki, Seiji Matsuda, Nobuaki Kobayashi

    Surgery   135 ( 6 )   604 - 12   2004.6

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    Background. Proliferation and apoptosis of liver cancer cells are closely related phenomena. We investigated the correlation between overexpression of Bcl-xL, an anti-apoptosis-related protein of the Bcl-2 family, and the clinical course of hepatocellular carcinoma (HCC).
    Methods. Specimens from 7 HCC patients were used for Western blotting and immunoelectron microscopy tests. Samples from 33 HCC patients who had undergone hepatectomies were used for immunohistochemical staining. The degrees of expression of Bcl-xL and Ki-67, as an index of HCC mitosis severity, were each classified into 2 groups.
    Results. With the use of Western blot analysis, enhanced immunoreactivity of Bcl-xL was found in cancerous specimens. Bcl-xL overexpression was found in cancer specimens in 21 of 33 patients (63.6%). The overall survival (P = .019) and disease-free survival (P = .030) rates of the group overexpressing Bcl-xL were definitely poorer. The Ki-67 higher labeling index LI > 10) group had a poorer survival rate (P = .016). There were significant correlations between Bcl-xL and overall survival and disease-free survival. Multivariate analyses revealed that. Bcl-xL, tumor size, histologic portal invasion, and histologic metastatic foci were independent prognostic factors for overall survival and disease-free survival.
    Conclusions. These results showed Bcl-xL in HCC specimens, suggesting that Bcl-xL was a significant Prognostic factor for disease progression in human HCC.

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  • Rho-ROCK signal pathway regulates microtubule-based process formation of cultured podocytes--inhibition of ROCK promoted process elongation. International journal

    Shuang-Yan Gao, Chun-Yu Li, Jie Chen, Lei Pan, Shouichiro Saito, Takehiro Terashita, Kyoko Saito, Kyojy Miyawaki, Kazuhiro Shigemoto, Katsumi Mominoki, Seiji Matsuda, Naoto Kobayashi

    Nephron. Experimental nephrology   97 ( 2 )   e49-61 - E61   2004

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    Background: Podocytes, renal glomerular visceral epithelial cells, have two kinds of processes, namely major processes containing microtubules (MTs) and foot processes with actin filaments (AFs). The present study investigated how MTs are organized by the Rho-ROCK signal transduction pathway during process formation of podocytes. Method: After induction of differentiation, podocytes of the conditionally immortalized mouse cell line were treated with Y-27632, a specific inhibitor of ROCK, and exoenzyme C3, an inhibitor of RhoA, as well as with forskolin whose effects include inhibition of RhoA, in order to inhibit the Rho-ROCK pathway. Results: Inhibition of ROCK significantly enhanced the formation of thick processes containing MT bundles. Y27632 promoted process formation even in the presence of latrunculin A which disrupts AFs, strongly suggesting that ROCK directly regulates MT assembly. Treatment with Y-27632 increased MT stability, and stabilized MTs preferentially localized in podocyte processes. Moreover, when treated with a combination of Y-27632 and forskolin, and with Y-27632 and C3 as well, podocytes developed not only MT-based thick processes but also AF-based thin projections. Conclusions: These data indicate a contribution of ROCK in MT organization to promote podocyte process formation, although it was originally thought to regulate AF assembly. AF-based thin projections seem to be induced mainly by inhibition of RhoA and ROCK. The present study reveals a significant role of the Rho-ROCK signal pathway in the reorganization of both MTs and AFs during process formation of podocytes. Copyright (C) 2004 S. Karger AG, Basel.

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  • Lectin histochemical study on the olfactory organ of the newt, Cynops pyrrhogaster, revealed heterogeneous mucous environments in a single nasal cavity. International journal

    Shouichiro Saito, Toshiyasu Matsui, Naoto Kobayashi, Hiroyuki Wakisaka, Katsumi Mominoki, Seiji Matsuda, Kazuyuki Taniguchi

    Anatomy and embryology   206 ( 5 )   349 - 56   2003.4

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    Expression patterns of glycoconjugates were examined by lectin histochemistry in the nasal cavity of the Japanese red-bellied newt, Cynops pyrrhogaster. Its nasal cavity consisted of two components, a flattened chamber, which was the main nasal chamber (MNC), and a lateral diverticulum called the lateral nasal sinus (LNS), which communicated medially with the MNC. The MNC was lined with the olfactory epithelium (OE), while the diverticulum constituting the LNS was lined with the vomeronasal epithelium (VNE). Nasal glands were observed beneath the OE but not beneath the VNE. In addition, a secretory epithelium was revealed on the dorsal boundary between the MNC and the LNS, which we refer to as the boundary secretory epithelium (BSE) in this study. The BSE seemed to play an important role in the construction of the mucous composition of the VNE. Among 21 lectins used in this study, DBA, SBA and Jacalin showed different staining patterns between the OE and the VNE. DBA staining showed remarkable differences between the OE and the VNE; there was intense staining, in the free border and the supporting cells of the VNE, whereas there was no staining or weak staining in the cells of the OE. SBA and Jacalin showed different stainings in the receptor neurons for the OE and the VNE. Furthermore, UEA-I and Con A showed different stainings for the nasal glands. UEA-I showed intense staining in the BSE and in the nasal glands located in the ventral wall of the MNC (VNG), whereas Con A showed intense staining in the BSE and in the nasal glands located in the dorsal and medial wall of the MNC (DMNG). The DMNG were observed to send their excretory ducts into the OE, whereas no excretory ducts were observed from the VNG to the OE or the VNE. These results suggested that the secretion by the supporting cells as well as the BSE and the DMNG establishes that there are heterogeneous mucous environments in the OE and the VNE, although both epithelia are situated in the same nasal cavity.

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  • 嗅上皮および鋤鼻器における神経栄養因子プロサポシンの発現性および発現部位についての検討

    齋藤 正一郎, 小林 直人, 脇坂 浩之, 樅木 勝巳, 宮脇 恭史, 齋藤 恭子, 陳 潔, 高 双燕, 佐野 輝, 松田 正司

    解剖学雑誌   78 ( Suppl. )   202 - 202   2003.4

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  • 動脈・静脈・リンパ管の解剖学

    メディカルレビュー社血管医学   4 ( 6 )   567 - 576   2003

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  • 細胞突起形成機構の分子形態学的解析

    生体の化学   54 ( 2 )   76 - 81   2003

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  • 両生類鋤鼻器の系統発生

    アニテックス   15 ( 3 )   103 - 108   2003

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  • Demyelination associated with HSV-1-induced facial paralysis. International journal

    Hiroyuki Wakisaka, Naohito Hato, Nobumitsu Honda, Hirotaka Takahashi, Hisanobu Kisaki, Shingo Murakami, Kiyofumi Gyo, Katsumi Mominoki, Naoto Kobayashi, Seiji Matsuda

    Experimental neurology   178 ( 1 )   68 - 79   2002.11

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    In 1995, we developed an animal model of transient homolateral facial nerve paralysis by inoculating Herpes simplex virus type 1 (HSV-1) into the auricle of mice. This study examined the mechanism of facial nerve paralysis in this model histopathologically. Using the immunofluorescence technique with anti-HSV-1 antibody, the time course of viral spread and the site of viral replication were investigated over the entire course of the facial nerve. Furthermore, viral replication and nerve degeneration at the site of viral replication were observed by electron microscopy. On the 7th day after inoculation, facial paralysis was observed in more than 60% of mice. Immunofluorescence study revealed HSV-1 in the geniculate ganglion, the descending root, and the facial nucleus at this stage. On the 9th day, the descending root in the sections stained with osmium. looked pale, because prominent demyelination had occurred in this region; electron micrographs showed many degenerated oligodendrocytes and large naked axons. In contrast, the facial nucleus neurons showed no remarkable degeneration, despite HSV-1 particles in their cytoplasm. From these findings, we concluded that facial nerve paralysis in this model is caused mainly by facial nerve demyelination in the descending root. (C) 2002 Elsevier Science (USA).

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  • Bcl-xL overexpression in human hepatocellular carcinoma. International journal

    Jota Watanabe, Fumiki Kushihata, Kazuo Honda, Katsumi Mominoki, Seiji Matsuda, Nobuaki Kobayashi

    International journal of oncology   21 ( 3 )   515 - 9   2002.9

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    It is known that proliferation and apoptosis are closely related phenomena in liver cancer cells. In this study, using surgical specimens from 42 patients with hepatocellular carcinoma (HCC), we investigated the expression and localization of Bcl-xL, an antiapoptosis-related protein of the Bcl-2 family. Using Western blotting, Bcl-xL expression was detected in both cancerous and non-cancerous specimens from all of the HCC patients, and elevated Bcl-xL levels were found in cancerous specimens from two thirds of the patients. In normal human liver specimens, Bcl-xL was found mainly in the cytoplasm of hepatocytes, although it was also found in the cytoplasm of bile duct cells in Glisson's capsule by immunohistochemical staining. To the best of our knowledge, this is the first report of Bcl-xL overexpression and localization in HCC specimens. Bcl-xL was found not only in the cytoplasm of HCC cells, but also in the nuclei of some HCC cells, suggesting that Bcl-xL is involved in the progression of HCC cells in vivo.

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  • ラット嗅球の生後発生における神経栄養因子プロサポシンの組織化学的局在

    齋藤 恭子, 齋藤 正一郎, 脇坂 浩之, 宮脇 恭史, 樅木 勝巳, 佐野 輝, 小林 直人, 松田 正司

    日本獣医学会学術集会講演要旨集   134回   171 - 171   2002.8

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  • ラット一次嗅覚系における神経栄養因子プロサポシンのmRNAの局在について

    齋藤 正一郎, 佐藤 康二, 齋藤 恭子, 樅木 勝巳, 留守 ゆう子, 佐野 輝, 脇坂 浩之, 宮脇 恭史, 小林 直人, 松田 正司

    日本獣医学会学術集会講演要旨集   134回   170 - 170   2002.8

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  • ラットの一次嗅覚系における神経栄養因子プロサポシンの局在について

    齋藤 正一郎, 樅木 勝巳, 齋藤 恭子, 小林 直人, 脇坂 浩之, 宮脇 恭史, 佐野 輝, 松田 正司

    日本獣医学会学術集会講演要旨集   132回   103 - 103   2001.9

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  • Herpes simplex virus in the vestibular ganglion and the geniculate ganglion-role of loose myelin. International journal

    H Wakisaka, N Kobayashi, K Mominoki, S Saito, N Honda, N Hato, K Gyo, S Matsuda

    Journal of neurocytology   30 ( 8 )   685 - 93   2001.8

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    This study presents the first direct evidence for herpes simplex virus type 1 (HSV-1) infection in the neurons of the vestibular ganglion. Although many investigators have reported electron microscopic evidence of HSV-1 infection in sensory ganglia, HSV-1 infection in the vestibular ganglion has not been described. Vestibular ganglion neurons have a unique structure, with a loose myelin sheath instead of the satellite cell sheath that is seen in other ganglia. This loose myelin is slightly different from compact myelin which is known as too tight for HSV-1 to penetrate. The role of loose myelin in terms of HSV-1 infection is completely unknown. Therefore, in an attempt to evaluate the role of loose myelin in HSV-1 infection, we looked for HSV-1 particles, or any effects mediated by HSV-1, in the vestibular ganglion as compared with the geniculate ganglion. At the light microscopic level, some neurons with vacuolar changes were observed, mainly in the distal portion of the vestibular ganglion where the communicating branch from the geniculate ganglion enters. At the electron microscopic level, vacuoles, dilated rough endoplasmic reticulum and Golgi vesicles occupied by virus were observed in both ganglia neurons. In contrast, viral infections in Schwann and satellite cells were observed only in the geniculate ganglion, but not in the vestibular ganglion. These results suggest that loose myelin is an important barrier to HSV-1 infection, and it must play an important role in the prevention of viral spread from infected neurons to other cells.

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  • ラット顔面神経切断後の顔面神経核内プロサポシンの増加

    鵜沼 香奈, 脇坂 浩之, 小林 直人, 齋藤 正一郎, 樅木 勝巳, 佐野 輝, 松田 正司

    解剖学雑誌   76 ( 1 )   92 - 92   2001.2

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  • ラット後腎組織培養系と阻害ペプチドを用いた、後腎の形態形成におけるラミニンの機能についての研究 Reviewed

    石原美佐, 野水基義, 長田道夫, 樅木勝巳, 脇坂浩之, 齋藤正一郎, 小林直人

    発達腎研究会誌   9   28 - 32   2001

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  • 愛媛大学医学部附属動物実験施設

    岡山実験動物研究会報   18   48 - 51   2001

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  • Morphogenetic activity of extracellular matrices on cultured podocytes. Laminin accelerates podocyte process formation in vitro.

    N Kobayashi, K Mominoki, H Wakisaka, Y Shimazaki, S Matsuda

    ADVANCES IN MICROANATOMY OF CELLS AND TISSUES, BIOPHYSICAL AND BIOCHEMICAL CORRELATES   7 ( 2 Suppl 1 )   423 - 430   2001

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    Morphogenetic effects of various extracellular matrix proteins on the renal podocyte were investigated using the conditionally immortalized podocyte cell line. Podocytes were plated on glass coverslips and coated with the following matrix proteins: laminin-10/11, laminin-1, fibronectin, collagen type IV, collagen type L Three hours after plating, podocytes on laminins developed prominent processes, while those on other matrix proteins started to elongate processes after two days. Vinculin-immunolabeling showed that podocytes plated on laminins possessed thin rod-shaped focal contacts, whereas those on fibronectin showed large dot-shaped focal contacts. Inhibition of serine/threonine protein kinases induced podocyte process formation in an extracellular matrix-independent manner. The present study reveals the significance of laminin on podocyte morphogenesis in vitro, and shows that different extracellular matrix proteins trigger different intracellular signals governing podocyte morphogenesis. Taken together with our previous studies, podocyte process formation is thought to be regulated by protein Ser/Thr phosphorylation.

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  • ラット後腎組織培養系を用いた,ラミニンの形態形成誘導能の研究

    石原 美佐, 小林 直人, 野水 基義, 長田 道夫, 山宮 公子, 樅木 勝巳, 脇坂 浩之, 島崎 由美子, 松田 正司

    解剖学雑誌   75 ( 5 )   478 - 478   2000.10

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  • ラット後腎の形態形成におけるラミニンの機能について 組織培養系を用いた研究

    石原 美佐, 小林 直人, 野水 基義, 長田 道夫, 山宮 公子, 樅木 勝巳, 脇坂 浩之, 島崎 由美子, 松田 正司

    解剖学雑誌   75 ( 1 )   61 - 61   2000.2

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  • Morphological transformation of sensory ganglion neurons and satellite cells

    Biomedical Reviews   11   39 - 52   2000

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  • 不死化細胞を用いた,細胞の形態形成へのアプローチー腎糸球体足細胞を例にしてー

    愛媛医学   19 ( 1 )   1 - 4   2000

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  • [Regulation of the microtubule-dependent process formation. A review based on a comparison between the neuron and the renal glomerular podocyte]. Reviewed

    N Kobayashi, K Mominoki, H Wakisaka, S Matsuda, T Sakai

    Kaibogaku zasshi. Journal of anatomy   74 ( 4 )   429 - 39   1999.8

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    Microtubular cytoskeletons play a crucial role in the morphogenesis of process-bearing cells, such as the neuron and the renal glomerular podocyte. Microtubules are bundled and stabilized by various microtubule-associated proteins, providing a mechanical basis to maintain the deviated morphology of cell processes. To support the process morphology, microtubules are also associated with other cytoskeletal elements such as actin and intermediate filaments. The microtubular polarity is uniformly plus-end-distal in neuronal axons, whereas in dendrites as well as in podocytes, the polarity is revealed to be non-uniform (i.e., both plus-end-distal and minus-end-distal microtubules are present in cell processes). Recently, this non-uniformity is reported to be established by a microtubule-dependent motor protein. Motor proteins are capable to drive the intracellular transport of cytoskeletal elements in addition to that of membrane vesicles. It is still an open question whether cytoskeletal elements are transported along cell processes as subunits or as polymers.

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  • Morphological changes of sensory neurons and perikaryal projections analysed by SEM

    Microscopy and Analysys   336   29 - 31   1999

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  • 糸球体足細胞における微小管の構築と機能ー培養細胞株を用いたアプローチー

    腎と透析   47 ( 6 )   849 - 853   1999

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  • 微小管に依存した細胞突起形成の調節機構ー神経細胞と糸球体足細胞との比較による考察ー

    日本解剖学会解剖学雑誌   74 ( 4 )   429 - 439   1999

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  • [Morphological transformation of sensory ganglion neurons and satellite cells]. Reviewed

    S Matsuda, N Kobayashi, K Mominoki, H Wakisaka, M Mori, S Murakami

    Kaibogaku zasshi. Journal of anatomy   73 ( 6 )   603 - 13   1998.12

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japanese Association of Anatomists  

    Sensory ganglion neurons in higher vertebrates are unique in that they are pseudounipolar with a single stem process that divides at some distance from the cell body into central and peripheral processes. In the early stages of development, these neurons are bipolar but later they became pseudounipolar. This developmental process of sensory ganglion neurons with satellite cells was examined by scanning electron microscopy following removal of connective tissue. This pseudo-unipolarization began earlier but proceeded at a slower rate in chick than in rat embryos. This difference may due to the difference found in the extent and intimacy of satellite cell investments in these two animals, which was due to the fact that sensory neurons undergo pseudo-unipolarization only in the presence of satellite cells in vitro. The neuronal perikaryal projections were observed by scanning electron microscopy after removal of connective tissue and satellite cells. Morphometric analysis revealed that perikaryal projections were more numerous on the surface of mature pseudounipolar neurons than on the surface of premature bipolar neurons, and that the number of projections increased as the neuronal cell bodies grew larger. This may support the hypothesis that perikaryal projections are structural devices for increasing the neuron-satellite interface and for improving the efficiency of metabolic exchange between these two cell types. These results suggest that satellite cells play an important role in neuronal maturation.

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  • 知覚神経節細胞の形態変化と衛星細胞の働き

    日本解剖学会解剖学雑誌   73 ( 6 )   603 - 613   1998

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  • Haptoglobin in the brown bear (Ursus arctos): molecular structure and hibernation related seasonal variations.

    MOMINOKI KATSUMI, MORIMATSU MASAMI, SAITO MASAYUKI

    家畜生化学   34 ( 2 )   45 - 53   1997.12

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  • Seasonal variations of blood haptoglobin level of brown bears in Japan. Reviewed International journal

    K Mominoki, H Tsuruga, M Morimatsu, M Saito

    Comparative biochemistry and physiology. Part A, Physiology   114 ( 4 )   349 - 53   1996.8

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    Haptoglobin (Hp), a hemoglobin-binding protein, is known as an acute phase protein increasing in blood during inflammation in most mammals. On the basis of our previous studies on purification and characterization of bear Hp (Comp. Biochem. Physiol. 110B, 785-789, 1995), in this study, we developed an immunoassay method to measure serum Hp level in bear, and measured the concentration of Hp in blood samples collected from 84 reared and 25 wild brown bears in Hokkaido, Japan. The mean serum Hp concentration was 0.94 +/- 0.25 mg/ml in wild bears, which is nearly equal to those reported in other species. In reared bears, the Hp concentration was apparently higher (3.82 +/- 0.29 mg/ml), although total protein and albumin concentrations were nearly equal in the two groups. A significant seasonal variation of serum Hp, low in spring and high in autumn and winter, was found in reared bears. Possible factors participating in the seasonal variation were discussed with special references to hibernation.

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  • Seasonal variations of blood haptoglobin level of brown bears in Japan

    K Mominoki, H Tsuruga, M Morimatsu, M Saito

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY   114 ( 4 )   349 - 353   1996.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    Haptoglobin (Hp), a hemoglobin-binding protein, is known as an acute phase protein increasing in blood during inflammation in most mammals. On the basis of our previous studies on purification and characterization of bear Hp (Comp. Biochem. Physiol. 110B, 785-789, 1995), in this study, we developed an immunoassay method to measure serum Hp level in bear, and measured the concentration of Hp in blood samples collected from 84 reared and 25 wild brown bears in Hokkaido, Japan. The mean serum Hp concentration was 0.94 +/- 0.25 mg/ml in wild bears, which is nearly equal to those reported in other species. In reared bears, the Hp concentration was apparently higher (3.82 +/- 0.29 mg/ml), although total protein and albumin concentrations were nearly equal in the two groups. A significant seasonal variation of serum Hp, low in spring and high in autumn and winter, was found in reared bears. Possible factors participating in the seasonal variation were discussed with special references to hibernation.

    DOI: 10.1016/0300-9629(96)00024-2

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  • Seasonal variation of blood haptoglobin level in brown bears

    University of New England PressAdaptations to the cold: tenth International Hibernation Symposium   1   397 - 372   1996

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  • Haptoglobin in Carnivora: a unique molecular structure in bear, cat and dog haptoglobins. Reviewed International journal

    K Mominoki, N Nakagawa-Tosa, M Morimatsu, B Syuto, M Saito

    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology   110 ( 4 )   785 - 9   1995.4

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    Haptoglobin (Hp), a hemoglobin-binding protein in plasma, consists of alpha and beta subunits and has a tetra-chain arrangement (beta-alpha-alpha-beta) connected by disulfide bridges in most mammals so far examined. Dog Hp has been reported to be unique compared with other Hps in respect that (1) the two alpha beta units are joined by a non-covalent interaction rather than a disulfide bridge and (2) the alpha chain has an oligosaccharide-binding sequence (Asn-X-Ser/Thr) and is glycosylated. To determine whether the unique structures of dog Hp are common in the Carnivora, we purified Hps from sera of bear and cat, and analyzed their subunit structure and partial amino acid sequences. The analyses by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, under both reducing and non-reducing conditions, revealed that bear and cat Hps have similar subunit arrangements to dog Hp, suggesting the absence of a disulfide bridge between two alpha chains. This was confirmed by amino acid sequence analysis of the alpha chains: that is, Cys15 participating in the inter-alpha chain disulfide bridge was replaced by Val in bear or Leu in cat and dog. Thus, the unique subunit arrangement of Hp reported in dog may be common in the Carnivora. In contrast to dog Hp, however, alpha chains of bear and cat Hps were found not to have the typical oligosaccharide binding sequence on their alpha chains and were not glycosylated.

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  • HAPTOGLOBIN IN CARNIVORA - A UNIQUE MOLECULAR-STRUCTURE IN BEAR, CAT AND DOG HAPTOGLOBINS

    K MOMINOKI, N NAKAGAWATOSA, M MORIMATSU, B SYUTO, M SAITO

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY   110 ( 4 )   785 - 789   1995.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    Haptoglobin (Hp), a hemoglobin-binding protein in plasma, consists of alpha and beta subunits and has a tetra-chain arrangement (beta-alpha-alpha-beta) connected by disulfide bridges in most mammals so far examined, Dog Hp has been reported to be unique compared with other Hps in respect that (1) the two alpha beta units are joined by a non-covalent interaction rather than a disulfide bridge and (2) the alpha chain has an oligosaccharide-binding sequence (Asn-X-Ser/Thr) and is glycosylated, To determine whether the unique structures of dog Hp are common in the Carnivora, we purified Hps from sera of bear and cat, and analyzed their subunit structure and partial amino acid sequences, The analyses by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, under both reducing and non-reducing conditions, revealed that bear and cat Hps have similar subunit arrangements to dog Hp, suggesting the absence of a disulfide bridge between two a chains, This was confirmed by amino acid sequence analysis of the alpha chains: that is, Cys(15) participating in the inter-a chain disulfide bridge was replaced by Val in bear or Leu in cat and dog, Thus, the unique subunit arrangement of Hp reported in dog may be common in the Carnivora, In contrast to dog Hp, however, alpha chains of bear and cat Hps were found not to have the typical oligosaccharide binding sequence on their alpha chains and were not glycosylated.

    DOI: 10.1016/0305-0491(94)00187-Y

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  • Isolation and Primary Culture of Bovine Hepatocytes: Albumin Synthesis and Adrenergic Activation of Glycogenosis

    Noriko-Tosa Nakagawa, Masami Morimatsu, Katsumi Mominoki, Bunei Syuto, Masayuki Saito

    Journal of Veterinary Medical Science   56 ( 1 )   125 - 129   1994

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    We describe a technique for isolation and primary culture of bovine hepatocytes, and their metabolic characterization. Hepatocytes were isolated from the caudate lobe of bovine liver by perfusion with 0.25 mM ethyleneglycol tetraacetic acid and 0.05% collagenase. The viability and yield of the cells were 70-92% and 0.1-3.6 x 107 cells/g liver, respectively. When the isolated hepatocytes were cultured in Williams medium E, they began to spread in 3 hr and formed monolayers in 24 hr. These monolayers were retained for at least 6 days. To monitor the metabolic activities specific to liver, synthesis and secretion of albumin were measured by labeling with [35S]-methionine and immunoprecipitation. This activity was low in isolated hepatocytes, but increased after culturing 1-3 days, and decreased again after 6 days. Glycogenolytic activity was also assessed by measuring glucose release to the medium by stimulation with epinephrine. The glycogenolytic response to epinephrine was also enhanced by culturing the hepatocytes 1-3 days, but was decreased after 6 days. Since the isolated bovine hepatocytes retained the liver-specific activities of albumin synthesis and glycogenolysis for several days in culture, these cells are useful for cellular and molecular studies on the functions of bovine liver. © 1994, JAPANESE SOCIETY OF VETERINARY SCIENCE. All rights reserved.

    DOI: 10.1292/jvms.56.125

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  • ハプトグロビンの分子構造に関する比較生化学的研究

    家畜生化学研究会報   30   23 - 30   1993

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  • ウシ肝細胞の分離および初代培養法

    家畜生化学研究会報   30 ( 2 )   51 - 56   1993

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MISC

  • セコバルビタールはペントバルビタールの代替候補薬として有用である

    赤木佐千子, 平山晴子, 矢田範夫, 石原すみれ, 橋本春菜, 樅木勝巳

    日本実験動物学会総会講演要旨集(Web)   67th   2020

  • コモンマーモセットにおける同居動物との争いによる創傷の治療の1例

    平山晴子, 矢田範夫, 橋本春菜, 樅木勝巳

    日本実験動物技術者協会総会講演要旨集   53rd   2019

  • コモンマーモセットを育てる

    橋本春菜, 矢田範夫, 赤木佐千子, 平山晴子, 樅木勝巳

    日本実験動物技術者協会総会講演要旨集   53rd   2019

  • ペントバルビタールナトリウムに代わる安楽死用薬の検討

    赤木佐千子, 平山晴子, 矢田範夫, 石原すみれ, 橋本春菜, 樅木勝巳

    日本実験動物技術者協会総会講演要旨集   53rd   2019

  • Helicobacter hepaticusの検疫方法に関する検討

    石原すみれ, 平山晴子, 樅木勝巳

    日本実験動物技術者協会総会講演要旨集   53rd   2019

  • 歯周病原細菌によるヒトと伴侶動物イヌとの人獣共通感染症検査の研究

    田井 真砂子, 伊東 孝, 平山 晴子, 矢田 範夫, 小川 寛人, 田村 和也, 伊東 有希, 大久保 圭祐, 伊東 昌洋, 中村 心, 岡本 憲太郎, 平井 公人, 山城 圭介, 大森 一弘, 山本 直史, 樅木 勝巳, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   135 - 135   2018.10

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  • 動物実験用としての手術支援ロボット“ダヴィンチ”の導入

    矢田範夫, 橋本春菜, 平山晴子, 樅木勝巳

    日本実験動物技術者協会総会講演要旨集   52nd   2018

  • 養豚農場から大学動物実験施設に搬入される家畜ブタのE型肝炎ウイルス感染状況について

    小川寛人, 平山晴子, 田中爽暉, 矢田範夫, 難波ひかる, 山下信子, 米満研三, 前田健, 樅木勝巳, 山田雅夫

    日本獣医学会学術集会講演要旨集   161st   2018

  • 岡山大学における研究者を対象としたマウス/ラット実技講習会開催の試み

    石原すみれ, 平山晴子, 赤木佐千子, 橋本春菜, 仲原生子, 矢田範夫, 上山和貴, 藤井匡寛, 樅木勝巳

    日本実験動物技術者協会総会講演要旨集   52nd   2018

  • 母マウスは哺育中の新生児が実験のために連れ去られることにどんなストレスを感じているのか?

    矢田範夫, 赤木佐千子, 石原すみれ, 橋本春菜, 樅木勝巳

    日本実験動物技術者協会総会講演要旨集   51st   2017

  • 実験のために動物を短距離・短時間移動させたとき,移動ストレスの回復にはどれぐらいの順化時間が必要か?

    橋本春菜, 矢田範夫, 石原すみれ, 赤木佐千子, 樅木勝巳

    日本実験動物技術者協会総会講演要旨集   51st   2017

  • e-learningシステムを用いた動物実験教育訓練知識確認試験

    矢田範夫, 上藤千佳, 平山晴子, 樅木勝巳

    生理学技術研究会報告・生物学技術研究会報告合同技術研究会報告   2017   2017

  • 子育て中の女性実験動物技術者による小規模施設の管理・運営~地域の子育て支援サービスの利用~

    石原すみれ, 平山晴子, 矢田範夫, 樅木勝巳

    日本実験動物技術者協会総会講演要旨集   51st   2017

  • e-learningによる動物実験教育訓練知識確認テストの実施

    矢田範夫, 矢田範夫, 上藤千佳, 上藤千佳, 平山晴子, 平山晴子, 樅木勝巳, 樅木勝巳

    日本実験動物技術者協会総会講演要旨集   49th   2015

  • 岡山大学における子育て支援制度とその利用の実際~契約職員の立場から~

    石原すみれ, 平山晴子, 矢田範夫, 樅木勝巳

    日本実験動物技術者協会総会講演要旨集   49th   2015

  • バーコードを利用した実験動物飼育管理データシステムの構築

    上藤千佳, 矢田範夫, 上山和貴, 荒川雅行, 藤井匡寛, 平山晴子, 樅木勝巳

    日本実験動物学会総会講演要旨集   61st   2014

  • 岡山大学自然生命科学研究支援センター動物資源部門津島北施設新営について

    樅木勝巳, 高嶋留美, 松川昭博

    日本実験動物学会総会講演要旨集   59th   2012

  • 血管新生における線維芽細胞の血管内皮細胞への分化転換に関する形態学的研究

    藤原隆, 昆和典, 樅木勝巳, 大沼俊名

    日本臨床分子形態学会総会ならびに学術講演会講演プログラム・予稿集   40th   2008

  • ヒヨコの開放性二分脊椎モデルにおける脊髄の感覚神経の走行の異常

    辻村隆介, 樅木勝巳, 寺下健洋, 下川哲哉, 絹谷政江, 松田正司

    解剖学雑誌   82 ( Supplement )   2007

  • 二分脊椎モデル動物の奇形脊髄領域の運動神経細胞の分化動態

    樅木勝巳, 王敏, 絹谷政江, 藤原隆, 小林直人, 松田正司

    日本獣医学会学術集会講演要旨集   144th   2007

  • ニワトリ二分脊椎におけるIslet-1陽性運動神経細胞の経時的変化

    王敏, 樅木勝巳, 絹谷政江, 松田正司, 藤原隆

    解剖学雑誌   82 ( Supplement )   2007

  • DOES WNT/β-CATENIN PATHWAY CONTROL THE STARFISH ARCHENTERON FORMATION THROUGH BRACHYURY?(Developmental Biology,Abstracts of papers presented at the 75^<th> Annual Meeting of the Zoological Society of Japan) :

    Miyawaki Kyojy, Doihara Takuya, Miguchi Yuji, Komori Hiroaki, Shigemoto Kazuhiro, Mominoki Katsumi, Ogasawara Masahito, Li Chun-yu, Chen Jie, Gao Shuang-yan, Saito Kyoko, Terashita Takehiro, Shimokawa Tetsuya, Saito Shouichiro, Kobayashi Naoto, Matsuda Seiji, Nose Masato

    Zoological science   21 ( 12 )   1294 - 1294   2004

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    Language:English   Publisher:Zoological Society of Japan  

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    Other Link: http://id.nii.ac.jp/1141/00038911/

  • MECHANISMS OF ARCHENTERON FORMATION IN STARFISH EMBRYOGENESIS : DETERMINATION OF VEGETAL POLE CELLS BY WNT/BETA-CATENIN PATHWAY(Developmental Biology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Miyawaki Kyojy, Pan Lei, Chen Jie, Gao Shuang-yan, Shigemoto Kazuhiro, Saito Kyoko, Terashita Takehiro, Mominoki Katsumi, Saito Shouichiro, Kobayashi Naoto, Matsuda Seiji

    Zoological science   20 ( 12 )   1558 - 1559   2003

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    Other Link: http://id.nii.ac.jp/1141/00037945/

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Presentations

  • ソムノペンチル®終売後のバルビツール酸誘導体の選択について Invited

    第66 回日本実験動物学会総会  2019.5 

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    Event date: 2019.5.15 - 2019.5.17

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Awards

  • 平成28年度「科研費」審査委員の表彰

    2016.9   日本学術振興会  

    樅木勝巳

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  • 第二回実験動物福祉奨励賞

    2011.9   日本実験動物技術者協会  

    原田 聡子, 矢田 範夫, 樅木 勝巳

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Research Projects

  • 1

    2020.12 - 2021.03

    株式会社アイエスディー  受託研究 

    モミノキカツミ

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  • 歩行障害を示す二分脊椎モデル動物における運動機能障害の病態に関する基礎的研究

    Grant number:20K08230  2020.04 - 2024.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    樅木 勝巳, 藤原 隆, 平山 晴子

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

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  • Pathophysiological research on the neural development of the affected spinal cord using the animal model of spina bifida

    Grant number:17K11509  2017.04 - 2020.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Mominoki Katsumi

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    This research using the animal model with spina bifida, which is one of the congenital malformation of a partial spinal cord, was operated to investigate the pathophysiology of the leg dysfunction in this model, using clues to understanding the nature of the neuronal development in the normal and/or abnormal the spinal cord region. On the other hand, in comparing the pathological condition of the spina bifida model animal, which is the final objective of this study, with the pathological condition of human spina bifida, the model used from the beginning caused inconvenience. Therefore, we decided to improve the method of creating the spina bifida model in this study. As a result, it has become possible to create a model that exhibits symptoms that are more similar to those of human spina bifida. It was suggested that the addition of this model to the experimental subjects may lead to more socially meaningful results.

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  • Basic research on the pathophysiological sequences of neural development on the affected spinal cord using the animal model of spina bifida aperta

    Grant number:25462774  2013.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Mominoki Katsumi, HIRAYAMA HARUKO

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    Grant amount:\4940000 ( Direct expense: \3800000 、 Indirect expense:\1140000 )

    This research was carried out to determine whether the neural disruption in spina bifida model animals with the leg dysfunction was occurred or not. the research suggested that the affected spinal cord to create spina bifida in the model lead to the neural disruption, regardless of the normal appearance of the developed spinal cord on the surface.

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  • 人工関節部材が関節軟骨部組織に及ぼす影響

    2012.12 - 2013.03

    ナカシマメディカル(株)R&Dセンター  受託研究 

    樅木勝巳, 平山晴子, 矢田範夫

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  • Basic research on the pathophysiological sequences of neural development on the affected spinal cord using the animal model of spina bifida aperta

    Grant number:22591979  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    MOMINOKI Katsumi, MATSUDA Seiji, FUJIWARA Takashi

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    This research was carried out to determine whether the neural disruption in spina bifida model animals with the leg dysfunction was occurred or not. the research suggested that the affected spinal cordto create spina bifida in the model lead to the neural disruption, regardless of the normal appearance of the developed spinal cord on the surface.

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  • Basic research on the pathophysiological sequences of neural development on the affected spinal cord using the animal model of spina bifida aperta for applying in utero surgery

    Grant number:19592059  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    MOMINOKI Katsumi, FUJIWARA Takashi

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    Grant amount:\4680000 ( Direct expense: \3600000 、 Indirect expense:\1080000 )

    脊髄先天性奇形の一つである二分脊椎症における奇形脊髄領域での神経細胞発生異常の有無に注目し、歩行障害を再現できる二分脊椎ニワトリモデルでの運動神経細胞の分化動態を免疫組織学的手法で検討した。その結果、運動神経細胞の発生の初期にわずかな発生遅滞が起こっていることが明らかとなった。これらの結果から二分脊椎症患者の歩行障害発生の抑制に胎児手術という母胎に負荷をかける治療方法の選択には、さらなる検討が必要であると結論できる。

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  • A study on the mechanism of induction of transdifferentiation of tissue-resident fibroblasts into endothelial cells, aiming at regenerative medicine

    Grant number:18591416  2006 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    FUJIWARA Takashi, MOMINOKI Katsumi, KON Kazunori, ONUMA Toshina, NOSE Masato

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    Grant amount:\4010000 ( Direct expense: \3500000 、 Indirect expense:\510000 )

    血管再生医療に必要な血管内皮細胞の入手先として結合組織常在性の線維芽細胞に注目し、線維芽細胞の血管内皮細胞への分化について遺伝子組換えマウスの角膜組織の移植や角膜細胞の培養を行って検討した。その結果、線維芽細胞の一種である角膜細胞は血管内皮細胞に分化・転換し、血管内皮に組み込まれること、線維芽細胞の内皮細胞への分化・転換は血管新生を起こしている組織に含まれる物質により誘導されることが明らかになり、血管内皮細胞の入手先として有望であると考えられた。

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  • Analysis of myasthenic disease caused by MuSK dysfunction

    Grant number:16590831  2004 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    SHIGEMOTO Kazuhiro, MOMINOKI Katsumi, MATSUDA Seiji, MARUYAMA Naoki, KUBO Sachiho

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    Grant amount:\3600000 ( Direct expense: \3600000 )

    Muscle-specific kinase (MuSK) is critical for the synaptic clustering of nicotinic acetylcholine receptors (AChR) and plays multiple roles in the organization and maintenance of neuromuscular junctions (NMJ). MuSK is activated by agrin, which is released from motoneurons, and induces AChR clustering at the postsynaptic membrane. Although autoantibodies against the ectodomain of MuSK have been found in a proportion of patients with generalized myasthenia gravis (MG), it is unclear whether MuSK autoantibodies are the causative agent of generalized MG. In the present study, rabbits immunized with MuSK ectodomain protein manifested MG-like muscle weakness with a reduction of AChR clustering at the NMJ. The autoantibodies activated MuSK and blocked AChR clustering induced by agrin or by mediators that do not activate MuSK. Thus, MuSK autoantibodies rigorously inhibit AChR clustering mediated by multiple pathways, an outcome that broadens our general comprehension of the pathogenesis of MG.

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  • 二分脊椎症に併発する歩行障害の病態解析:二足歩行二分脊椎モデルを用いた基礎的研究

    Grant number:16790846  2004 - 2005

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    樅木 勝巳

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    Grant amount:\3500000 ( Direct expense: \3500000 )

    椎弓の欠損を主徴とする非致死性脊髄奇形である二分脊椎症は、主に椎弓欠損のみの潜在性型と椎弓欠損に髄膜あるいは脊髄と髄膜の両方が巻き込まれる嚢胞性型とに大別される。一般に潜在性型は無症状であるが、嚢抱性型は種々の程度の神経障害を示す。これまでの二分脊椎症に関連する研究では、この神経障害の病態は臨床知見に基づいたものがほとんどであり、実験に基づく知見は臨床知見に比べて少ない。したがって、その詳細な病態については不明な点が未だ多く残されている。
    我々はこれまでに二分脊椎症で見られる神経障害の病態を詳細に解析することを目的として、ヒト二分脊椎症患者に似た後肢運動障害を示す二分脊椎モデル動物を開発した。平成17年度では、平成16年度の研究において見いだされた神経細胞の発生障害についてより詳細な知見を得るための実験を行った。すなわち、運動神経細胞のサブクラスマーカーとして知られている数種類の転写調節因子に対するモノクローナル抗体を用いて、本モデルの脊髄奇形領域における運動神経細胞サブクラスを染め分け、これらの数の変化及び脊髄内での神経細胞の分布変化を追跡した。
    本年度に得た結果では、本モデルにおいて、奇形例の運動神経細胞の発生は約1〜2日程度の遅延がみられるが、最終的には正常発生例とほぼ同数の運動神経細胞が発生することが明らかとなった。しかし、神経細胞の脊髄内の空間分布には奇形例と二分脊椎例で違いが見られた。さらに、内転筋にWGA-HRPを注入し、脊髄内のHRP陽性細胞数をカウントしたところ、この陽性細胞の随節毎の分布にも奇形例と二分脊椎例で違いが見られた。これらの結果から発生途上の神経管が羊水環境に暴露されることによって、脊髄神経細胞の空間的な分布に異常をし、その結果として本モデルにおいて歩行障害を引き起こしていることが予測される。

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  • An analysis of the induction mechanism of transdifferentiation of connective tissue-resident fibroblasts into endothelial cells of blood vessels

    Grant number:16591260  2004 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    FUJIWARA Takashi, MOMINOKI Katsumi, KON Kazunori, NOSE Masato

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    Grant amount:\3600000 ( Direct expense: \3600000 )

    [Introduction]
    Recently, bone marrow-derived endothelial progenitor cells and ES cells have been paid attention on as a source of vascular endothelial cells for regenerative therapy. However, the use of these cells has been pointed out to have immunological and ethical problems. In this study we examined whether connective tissue-resident fibroblasts are able to transdifferentiate into endothelial cells of blood vessels.
    [Materials and Methods]
    We carried out two experiments. In one experiment, a small piece of tissue graft was excised from corneal stroma of Tg mice of which endothelial cells of blood vessels expressed a lacZ gene, and was transplanted together with sarcoma cells into a cornea pocket of WT mice. In another experiment, corneal stroma cells (fibroblasts) from Tg mice were cultured, expanded and transfused into tail veins of WT mice whose cornea was engrafted with sarcoma beforehand to induce angiogenesis. After new blood vessels grew into the cornea, the cornea was processed for X-Gal staining. Here we used corneal stroma as a material, because a cornea contains no blood vessels and a corneal stroma consists of only stroma cells, fiblobrasts as cell components.
    [Result]
    In both experiments, a few lacZ positive cells were found in the endothelium of the newly formed blood vessel in the cornea.
    [Discussion]
    This result suggests that connective tissue-resident fibroblasts are a hopeful source of endothelial cells for regenerative therapy, gene therapy of blood vessel or for seeding on and lining the inner surface of vascular prosthetic grafts.

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  • Leg dysfunctions in a chick model of spina bifida aperta

    Grant number:14370466  2002 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    MATSUDA Seiji, KOBAYASHI Naoto, SANO Akira, MOMINOKI Katsumi, SAITO Shouichirou

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    Grant amount:\12100000 ( Direct expense: \12100000 )

    We created chicks with spina bifida aperta (SBA) by incising the roof plate of the neural tube of embryos at Hamburger and Hamilton stage 18 or 19. Incision over the length of five and seven somites caused SBA-like malformation in 100% of the hatchlings. The SBO chicks exhibited no symptoms, whereas the SBA chicks exhibited paralysis of a leg muscle and imbalance between an agonist and an antagonist leg muscles. Lesions in these SBA chicks were located in the spinal segments that give rise to motor neurons that innervated the dysfunctional muscles. Histological analysis revealed that there were fewer small spinal interneurons at the site of the lesion in SBA chicks than in the normal chicks and that there was no such difference in the number of the large spinal motor neurons. Leg dysfunctions in this model of SBA may be attributable to the smaller number of interneurons in the spinal segments that contain motor neurons that innervate the dysfunctional muscle. This model may facilitate studies of the pathological mechanisms that lead to leg dysfunctions in SBA chicks. We examined the SBA neural tube in by immuno- histochemical staining with the monoclonal antibody against Islet-1. The number and distribution of the Islet-1 positive motoneurons were determined at the position equivalent in the Lumbar 3rd vertebra and compared between SBA groups and control groups. On E4 and E4.5, the motoneuron number in the SBA group was less than that in the control group. On E6, the difference in the numbers of Islet-1 positive neuron between SBA group and control group was not significant. Moreover, the distribution of the Islet-1 positive cells in SBA group was different from that of control group on E6. These results suggest that the development of motoneuron is delayed in the chick embryos with SBA.

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  • 二分脊椎症で併発する下肢の変形及び運動障害の発症メカニズムに関する基礎的研究

    Grant number:14770984  2002 - 2003

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    樅木 勝巳

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    Grant amount:\3800000 ( Direct expense: \3800000 )

    椎弓及び棘突起の先天性欠損症である二分脊椎症は、これらの欠損に脊髄や硬膜の奇形を伴わない潜在性型と欠損が伴う嚢胞性型に大別される。日本における二分脊椎症の発生頻度は5000出産に対して2〜3例であるといわれており、おおよそ潜在性型:嚢胞性型=3:7である。一般に潜在性型は無症状であるが、嚢抱性型は種々の程度の神経症状を示すことが知られている。これらの神経症状の発症原因は明らかになっていないが、二分脊椎患者では神経症状と脊髄組織の欠損程度及び位置との相関が見られることから、二分脊椎症に併発する神経症状の原因は胎生期における神経の損傷であると考えられている。
    我々はこれまでに二分脊椎症で見られる神経損傷の病態を詳細に解析するためにヒト二分脊椎症患者に似た後肢の運動障害や骨変形を示す二分脊椎ヒヨコを開発した。平成15年度では、このモデルにおける脊髄奇形領域における脊髄神経、特に運動神経に変化について焦点を合わせ、コリン作動性神経細胞のマーカーであるアセチルコリントランスフエラーゼの特異抗体を用い、二分脊椎ヒヨコの奇形脊髄の免疫染色を行った。すなわち、二分脊椎例及び正常発生例、それぞれ5例の第3腰髄の15μm厚の連続切片を作製し、各個体につき3切片毎(30μm毎)・25切片上の抗体陽性細胞数を数え、二分脊椎例では872.5±48.2個、正常発生例では752.0±37.0個であった。この結果から二分脊椎ヒヨコの奇形脊髄領域には正常発生例とほぼ同数の機能的な運動神経細胞が存在していることを示唆され、本モデルで見られる後肢の運動機能障害が運動神経細胞の機能異常によるものでなく、脊髄介在神経細胞などの運動神経の上位にある機能調整系の機能不全によって引き起こされたものであると結論した。

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  • 二分脊椎症時の神経管発生機構に関する研究

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  • The body defense system in hypothermia during hibernation

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  • 非免疫性血清因子による原虫排除機構に関する研究

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  • The mechanism of elimination of protozoo by non-immano factors in the blood

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  • The neural development in the spirul bifida chickens

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  • 冬眠動物における生体防御機構に関する研究

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