Updated on 2026/01/06

写真a

 
IDE Toru
 
Organization
Faculty of Interdisciplinary Science and Engineering in Health Systems Professor
Position
Professor
External link

Degree

  • doctor (science) ( 1992.3   Osaka University )

  • PhD ( 1992.3   Osaka University )

Research Interests

  • photoactivated enzyme

  • channel protein

  • single molecule measurement

Research Areas

  • Life Science / Biophysics  / channel protein

Education

  • Osaka University   基礎工学研究科  

    1985.4 - 1991.9

    　 More details

  • Kyoto University   理学部  

    1980.4 - 1985.3

    　 More details

Research History

  • 岡山大学大学院ヘルスシステム統合科学研究科   教授

    2018.4

    　 More details

  • Okayama University   Faculty of Engineering   Professor

    2013.8

    　 More details

 

Papers

  • Direct insertion of an ion channel immobilized on a soft agarose gel bead into a lipid bilayer: an optimized method

    Mami Asakura, Shuyan Wang, Minako Hirano, Toru Ide

    Analytical Sciences   41 ( 7 )   1073 - 1082   2025.5

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    In this paper, we report the development of a device that improves the conventional artificial lipid bilayer method and can measure channel currents more efficiently. Ion channel proteins are an attractive research target in biophysics, because their functions can be measured at the single-molecule level with high time resolution. In addition, they have attracted attention as targets for drug discovery because of their crucial roles in vivo. Although electrophysiological methods are powerful tools for studying channel proteins, they suffer from low measurement efficiency and require considerable skill. In our previous paper, we reported that by immobilizing channel proteins on agarose gel beads and forming an artificial lipid bilayer on the bead surface, we simultaneously solved two problems that had been hindering the efficiency of the artificial bilayer method: the time-consuming formation of artificial lipid bilayers and the time-consuming incorporation of channels into artificial bilayers. Previous studies have utilized crosslinked hard beads; however, here we show that channel current measurement can be achieved more simply and efficiently using non-crosslinked soft beads. In this study, we detailed the process of immobilizing channel proteins on the surface of non-crosslinked beads through chemical modification, allowing us to measure their channel activity. This method enables current measurements without the need for stringent bead size selection or high negative pressure.

    Graphical abstract

    DOI： 10.1007/s44211-025-00792-y

    researchmap

    Other Link： https://link.springer.com/article/10.1007/s44211-025-00792-y/fulltext.html

  • Potency of agarose gel‐supported lipid bilayers for electrophysiologic analysis of channel pores formed by Bacillus thuringiensis insecticidal proteins

    Tsubasa Okuda, Tomoya Takeuchi, Mami Asakura, Minako Hirano, Toru Ide, Tohru Hayakawa

    The FEBS Journal   2025.3

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    Electrophysiologic analysis using artificial lipid bilayers is useful for studying the formation of pores by insecticidal proteins, especially the ion permeability of toxin pores. However, such studies are time‐consuming and require special skills, particularly regarding the construction of lipid bilayers and promoting toxin pore formation. To facilitate the analysis of toxin pore formation in the present study, we evaluated the usefulness of agarose gel‐supported lipid bilayers for electrophysiologic measurements using two structurally different mosquito‐larvicidal proteins, Mpp46Ab and Cry4Aa. The agarose gel‐supported lipid bilayers enabled the measurement of channel currents through pores made by both toxins and, notably, the lipid bilayers could be easily reconstructed even after disruption of the lipid bilayer. Using this system, measurements could be repeated at least five times using the same apparatus and toxins. We also investigated the effect of the lipid bilayer component on toxin pore formation and found that the incorporation of both cholesterol and sphingomyelin into the lipid bilayer facilitates the formation of pores by both Mpp46Ab and Cry4Aa. Both cholesterol and sphingomyelin are major components of lipid raft microdomains, suggesting that, in addition to recruiting toxin receptors, raft microdomains play a key role in membrane insertion and pore formation by insecticidal proteins.

    DOI： 10.1111/febs.70070

    researchmap

  • Efficient single-channel current measurements of the human BK channel using a liposome-immobilized gold probe

    Minako Hirano, Mami Asakura, Toru Ide

    Analytical Sciences   41 ( 4 )   329 - 334   2024.12

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    The human BK channel (hBK) is an essential membrane protein that regulates various biological functions, and its dysfunction leads to serious diseases. Understanding the biophysical properties of hBK channels is crucial for drug development. Artificial lipid bilayer recording is used to measure biophysical properties at the single-channel level. However, this technique is time-consuming and complicated; thus, its measurement efficiency is very low. Previously, we developed a novel technique to improve the measurement efficiency by rapidly forming lipid bilayer membranes and incorporating ion channels into the membrane using a hydrophilically modified gold probe. To further improve our technique for application to the hBK channel, we combined it using the gold probe with a liposome fusion method. Using a probe on which liposomes containing hBK channels were immobilized, the channels were efficiently incorporated into the lipid bilayer membrane, and the measured channel currents showed the current characteristics of the hBK channel. This technique will be useful for the efficient measurements of the channel properties of hBK and other biologically important channels.

    Graphical abstract

    DOI： 10.1007/s44211-024-00707-3

    researchmap

    Other Link： https://link.springer.com/article/10.1007/s44211-024-00707-3/fulltext.html

  • Mutational analysis of the transmembrane α4-helix of Bacillus thuringiensis mosquito-larvicidal Cry4Aa toxin. International journal

    Hirokazu Takahashi, Mami Asakura, Toru Ide, Tohru Hayakawa

    Current microbiology   81 ( 3 )   80 - 80   2024.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Cry4Aa, produced by Bacillus thuringiensis subsp. israelensis, exhibits specific toxicity to larvae of medically important mosquito genera. Cry4Aa functions as a pore-forming toxin, and a helical hairpin (α4-loop-α5) of domain I is believed to be the transmembrane domain that forms toxin pores. Pore formation is considered to be a central mode of Cry4Aa action, but the relationship between pore formation and toxicity is poorly understood. In the present study, we constructed Cry4Aa mutants in which each polar amino acid residues within the transmembrane α4 helix was replaced with glutamic acid. Bioassays using Culex pipiens mosquito larvae and subsequent ion permeability measurements using symmetric KCl solution revealed an apparent correlation between toxicity and toxin pore conductance for most of the Cry4Aa mutants. In contrast, the Cry4Aa mutant H178E was a clear exception, almost losing its toxicity but still exhibiting a moderately high conductivity of about 60% of the wild-type. Furthermore, the conductance of the pore formed by the N190E mutant (about 50% of the wild-type) was close to that of H178E, but the toxicity was significantly higher than that of H178E. Ion selectivity measurements using asymmetric KCl solution revealed a significant decrease in cation selectivity of toxin pores formed by H178E compared to N190E. Our data suggest that the toxicity of Cry4Aa is primarily pore related. The formation of toxin pores that are highly ion-permeable and also highly cation-selective may enhance the influx of cations and water into the target cell, thereby facilitating the eventual death of mosquito larvae.

    DOI： 10.1007/s00284-023-03602-8

    PubMed

    researchmap

  • Random Mutational Analysis Targeting Residue K155 within the Transmembrane β-Hairpin of the Mosquitocidal Mpp46Ab Toxin

    Midoka Miyazaki, Mami Asakura, Toru Ide, Tohru Hayakawa

    Biology   12 ( 12 )   1481 - 1481   2023.12

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Mpp46Ab is a mosquito-larvicidal pore-forming toxin derived from Bacillus thuringiensis TK-E6. Pore formation is believed to be a central mode of Mpp46Ab action, and the cation selectivity of the channel pores, in particular, is closely related to its mosquito-larvicidal activity. In the present study, we constructed a mutant library in which residue K155 within the transmembrane β-hairpin was randomly replaced with other amino acid residues. Upon mutagenesis and following primary screening using Culex pipiens mosquito larvae, we obtained 15 mutants in addition to the wild-type toxin. Bioassays using purified proteins revealed that two mutants, K155E and K155I, exhibited toxicity significantly higher than that of the wild-type toxin. Although increased cation selectivity was previously reported for K155E channel pores, we demonstrated in the present study that the cation selectivity of K155I channel pores was also significantly increased. Considering the characteristics of the amino acids, the charge of residue 155 may not directly affect the cation selectivity of Mpp46Ab channel pores. Replacement of K155 with glutamic acid or isoleucine may induce a similar conformational change in the region associated with the ion selectivity of the Mpp46Ab channel pores. Mutagenesis targeting the transmembrane β-hairpin may be an effective strategy for enhancing the ion permeability of the channel pores and the resulting mosquito-larvicidal activity of Mpp46Ab.

    DOI： 10.3390/biology12121481

    researchmap

  • 単一イオンチャンネルの簡便計測システム

    Minako HIRANO, Mami ASAKURA, Toru IDE

    Seibutsu Butsuri   63 ( 2 )   110 - 114   2023

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Biophysical Society of Japan  

    DOI： 10.2142/biophys.63.110

    researchmap

  • Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis

    Shintaro Mise, Akinobu Matsumoto, Keisuke Shimada, Toshiaki Hosaka, Masatomo Takahashi, Kazuya Ichihara, Hideyuki Shimizu, Chisa Shiraishi, Daisuke Saito, Mikita Suyama, Tomoharu Yasuda, Toru Ide, Yoshihiro Izumi, Takeshi Bamba, Tomomi Kimura-Someya, Mikako Shirouzu, Haruhiko Miyata, Masahito Ikawa, Keiichi Nakayama

    NATURE COMMUNICATIONS   13 ( 1 )   2022.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41467-022-28677-y

    Web of Science

    researchmap

  • Characteristics of channel pores formed by Bacillus thuringiensis mosquito-larvicidal Cry4Aa toxin

    Yuri Shiraishi, Tomoya Shiozaki, Mami Asakura, Toru Ide, Tohru Hayakawa

    Applied Entomology and Zoology   57 ( 1 )   63 - 70   2021.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s13355-021-00762-6

    Web of Science

    researchmap

    Other Link： https://link.springer.com/article/10.1007/s13355-021-00762-6/fulltext.html

  • Development of an automated system to measure ion channel currents using a surface-modified gold probe. International journal

    Minako Hirano, Masahisa Tomita, Chikako Takahashi, Nobuyuki Kawashima, Toru Ide

    Scientific reports   11 ( 1 )   17934 - 17934   2021.9

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41598-021-97237-z

    Web of Science

    PubMed

    researchmap

  • Low-Light Photodetectors for Fluorescence Microscopy

    Hiroaki Yokota, Atsuhito Fukasawa, Minako Hirano, Toru Ide

    Applied Sciences   11 ( 6 )   2773 - 2773   2021.3

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/app11062773

    Web of Science

    researchmap

  • A Lipid Bilayer Formed on a Hydrogel Bead for Single Ion Channel Recordings. Reviewed International journal

    Minako Hirano, Daiki Yamamoto, Mami Asakura, Tohru Hayakawa, Shintaro Mise, Akinobu Matsumoto, Toru Ide

    Micromachines   11 ( 12 )   1070   2020.12

    　More details

    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/mi11121070

    Web of Science

    PubMed

    researchmap

  • Channel-pore cation selectivity is a major determinant of Bacillus thuringiensis Cry46Ab mosquitocidal activity. International journal

    Tohru Hayakawa, Midoka Miyazaki, Syoya Harada, Mami Asakura, Toru Ide

    Applied microbiology and biotechnology   104 ( 20 )   8789 - 8799   2020.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-020-10893-5

    Web of Science

    PubMed

    researchmap

  • Single-molecule imaging of PI(4,5)P2 and PTEN in vitro reveals a positive feedback mechanism for PTEN membrane binding. International journal

    Daisuke Yoshioka, Seiya Fukushima, Hiroyasu Koteishi, Daichi Okuno, Toru Ide, Satomi Matsuoka, Masahiro Ueda

    Communications biology   3 ( 1 )   92 - 92   2020.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s42003-020-0818-3

    Web of Science

    PubMed

    researchmap

  • The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata. International journal

    Minako Hirano, Masumi Takebe, Tomoya Ishido, Toru Ide, Shigeru Matsunaga

    Scientific reports   9 ( 1 )   20262 - 20262   2019.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41598-019-56721-3

    Web of Science

    PubMed

    researchmap

  • Characterization of the channel-pores formed by Bacillus thuringiensis Cry46Ab toxin in planar lipid bilayers

    Akira Sakakibara, So Takebe, Toru Ide, Tohru Hayakawa

    Applied Entomology and Zoology   54 ( 4 )   389 - 398   2019.11

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s13355-019-00635-z

    Web of Science

    researchmap

    Other Link： http://link.springer.com/article/10.1007/s13355-019-00635-z/fulltext.html

  • Electrostatic state of the cytoplasmic domain influences inactivation at the selectivity filter of the KcsA potassium channel. International journal

    Minako Hirano, Toru Ide

    Biochimica et biophysica acta. Biomembranes   1861 ( 1 )   220 - 227   2019.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbamem.2018.07.011

    Web of Science

    PubMed

    researchmap

  • A gold nano-electrode for single ion channel recordings. International journal

    Daichi Okuno, Minako Hirano, Hiroaki Yokota, Junya Ichinose, Takamitsu Kira, Taiki Hijiya, Chihiro Uozumi, Masahiro Yamakami, Toru Ide

    Nanoscale   10 ( 8 )   4036 - 4040   2018.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/c7nr08098k

    Web of Science

    PubMed

    researchmap

  • Potency of the mosquitocidal Cry46Ab toxin produced using a 4AaCter-tag, which facilitates formation of protein inclusion bodies in Escherichia coli

    Tomoaki Okazaki, Junya Ichinose, So Takebe, Toru Ide, Tohru Hayakawa

    APPLIED ENTOMOLOGY AND ZOOLOGY   53 ( 1 )   67 - 73   2018.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s13355-017-0529-5

    Web of Science

    researchmap

  • Cry46Ab from Bacillus thuringiensis TK-E6 is a new mosquitocidal toxin with aerolysin-type architecture. International journal

    Tohru Hayakawa, Akira Sakakibara, Sho Ueda, Yoshinao Azuma, Toru Ide, So Takebe

    Insect biochemistry and molecular biology   87   100 - 106   2017.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2017.06.015

    Web of Science

    PubMed

    researchmap

  • Bacillus thuringiensis Cry11Ba works synergistically with Cry4Aa but not with Cry11Aa for toxicity against mosquito Culex pipiens (Diptera: Culicidae) larvae

    Tohru Hayakawa, Naoya Yoneda, Kouji Okada, Ayuko Higaki, Mohammad Tofazzal Hossain Howlader, Toru Ide

    Applied Entomology and Zoology   52 ( 1 )   61 - 68   2017.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s13355-016-0454-z

    Web of Science

    researchmap

    Other Link： http://link.springer.com/article/10.1007/s13355-016-0454-z/fulltext.html

  • Liposome chaperon in cell-free membrane protein synthesis: one-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method. Reviewed International journal

    M Ando, M Akiyama, D Okuno, M Hirano, T Ide, S Sawada, Y Sasaki, K Akiyoshi

    Biomaterials science   4 ( 2 )   258 - 64   2016.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/c5bm00285k

    Web of Science

    Scopus

    PubMed

    researchmap

  • A Simple Method for Ion Channel Recordings Using Fine Gold Electrode.

    Daichi Okuno, Minako Hirano, Hiroaki Yokota, Yukiko Onishi, Junya Ichinose, Toru Ide

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   32 ( 12 )   1353 - 1357   2016

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.2116/analsci.32.1353

    Web of Science

    PubMed

    researchmap

  • A single amino acid gates the KcsA channel. International journal

    Minako Hirano, Daichi Okuno, Yukiko Onishi, Toru Ide

    Biochemical and biophysical research communications   450 ( 4 )   1537 - 40   2014.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2014.07.032

    Web of Science

    PubMed

    researchmap

  • 3P222 Modifications of ion channel function(Biological & Artificial membrane: Excitation & Channels,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Minako Hirano, Daichi Okuno, Yukiko Onishi, Hiroaki Yokota, Toru Ide

    Seibutsu Butsuri   54 ( 1 )   S285   2014

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.54.S285_6

    CiNii Article

    researchmap

  • Uncovering the protein translocon at the chloroplast inner envelope membrane. International journal

    Shingo Kikuchi, Jocelyn Bédard, Minako Hirano, Yoshino Hirabayashi, Maya Oishi, Midori Imai, Mai Takase, Toru Ide, Masato Nakai

    Science (New York, N.Y.)   339 ( 6119 )   571 - 4   2013.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1126/science.1229262

    Web of Science

    PubMed

    researchmap

  • The KcsA Channel Cytoplasmic Domain Effects on the Inactivation Gating

    Minako Hirano, Yukiko Onishi, Daichi Okuno, Toru Ide

    BIOPHYSICAL JOURNAL   104 ( 2 )   128A - 128A   2013.1

    　More details

  • Automated parallel recordings of topologically identified single ion channels. International journal

    Ryuji Kawano, Yutaro Tsuji, Koji Sato, Toshihisa Osaki, Koki Kamiya, Minako Hirano, Toru Ide, Norihisa Miki, Shoji Takeuchi

    Scientific reports   3   1995 - 1995   2013

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/srep01995

    Web of Science

    PubMed

    researchmap

  • Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level

    R. Kawano, Y. Tsuji, M. Hirano, T. Osaki, K. Kamiya, N. Miki, T. Ide, S. Takeuchi

    Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)   57 - 58   2013

    　More details

    Language：English   Publishing type：Research paper (international conference proceedings)  

    DOI： 10.1109/MEMSYS.2013.6474175

    Scopus

    researchmap

  • A glass fiber sheet-based electroosmotic lateral flow immunoassay for point-of-care testing. International journal

    Yuriko Oyama, Toshihisa Osaki, Koki Kamiya, Ryuji Kawano, Tsutomu Honjoh, Haruki Shibata, Toru Ide, Shoji Takeuchi

    Lab on a chip   12 ( 24 )   5155 - 9   2012.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/c2lc40994a

    Web of Science

    PubMed

    researchmap

  • Mechanically Manipulating a Single Channel Gate

    IDE Toru, HIRANO Minako, OKUNO Daichi

    Seibutsu Butsuri   52 ( 6 )   289 - 290   2012.11

    　More details

    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.52.289

    CiNii Article

    CiNii Books

    J-GLOBAL

    researchmap

  • Glass fiber sheet on a chip: For rapid, low-cost, and contamination-free quantitative immunoassay

    Yuriko Oyama, Toshihisa Osaki, Koki Kamiya, Ryuji Kawano, Tsutomu Honjoh, Haruki Shibata, Toru Ide, Shoji Takeuchi

    Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012   1957 - 1959   2012

    　More details

    Language：English   Publishing type：Research paper (international conference proceedings)   Publisher：Chemical and Biological Microsystems Society  

    Scopus

    researchmap

  • Role of the KcsA channel cytoplasmic domain in pH-dependent gating. International journal

    Minako Hirano, Yukiko Onishi, Toshio Yanagida, Toru Ide

    Biophysical journal   101 ( 9 )   2157 - 62   2011.11

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bpj.2011.09.024

    Web of Science

    PubMed

    researchmap

  • Direct manipulation of a single potassium channel gate with an atomic force microscope probe. International journal

    Mitsunori Kitta, Toru Ide, Minako Hirano, Hiroyuki Tanaka, Toshio Yanagida, Tomoji Kawai

    Small (Weinheim an der Bergstrasse, Germany)   7 ( 16 )   2379 - 83   2011.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/smll.201002337

    Web of Science

    PubMed

    researchmap

  • Channels formed by amphotericin B covalent dimers exhibit rectification. International journal

    Minako Hirano, Yuko Takeuchi, Nobuaki Matsumori, Michio Murata, Toru Ide

    The Journal of membrane biology   240 ( 3 )   159 - 64   2011.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00232-011-9354-x

    Web of Science

    PubMed

    researchmap

  • The Role of the Cytoplasimic Domain in pH-Dependent Gating by the KCSA Channel

    Minako Hirano, Toru Ide

    BIOPHYSICAL JOURNAL   100 ( 3 )   273 - 273   2011.2

    　More details

    Language：English  

    Web of Science

    researchmap

  • 3A1024 Mechanism for opening and closing by the KcsA channel(3A Biol & Artifi memb 3: Excitation & Channels,The 49th Annual Meeting of the Biophysical Society of Japan)

    Hirano Minako, Onishi Yukiko, Yanagida Toshio, Ide Toru

    Seibutsu Butsuri   51   S105   2011

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.51.S105_1

    CiNii Article

    researchmap

  • 2SH-06 Direct Manipulation of a Single Channel Gate with an AFM Probe(2SH New Experimental Tools for Structural Changes of Membrane Proteins: Beyond X-ray Structures,The 49th Annual Meeting of the Biophysical Society of Japan)

    Ide Toru, Hirano Minako, Okuno Daichi, Kitta Mitsunori

    Seibutsu Butsuri   51   S21   2011

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.51.S21_1

    CiNii Article

    researchmap

  • Visualization of the COPII vesicle formation process reconstituted on a microscope Reviewed

    Kazuhito V. Tabata, Ken Sato, Toru Ide, Hiroyuki Noji

    Cell Signaling Reactions: Single-Molecular Kinetic Analysis   167 - 182   2011

    　More details

    Language：English   Publishing type：Part of collection (book)   Publisher：Springer Netherlands  

    DOI： 10.1007/978-90-481-9864-1_8

    Scopus

    researchmap

  • Simultaneous optical and electrical recording of single molecule bonding to single channel proteins. International journal

    Toru Ide

    Chemphyschem : a European journal of chemical physics and physical chemistry   11 ( 16 )   3408 - 11   2010.11

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/cphc.201000560

    Web of Science

    PubMed

    researchmap

  • Simultaneous optical and electrical single channel recordings on a PEG glass. International journal

    Toru Ide, Yuko Takeuchi, Hiroyuki Noji, Kazuhito V Tabata

    Langmuir : the ACS journal of surfaces and colloids   26 ( 11 )   8540 - 3   2010.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/la9045594

    Web of Science

    PubMed

    researchmap

  • Rearrangements in the KcsA cytoplasmic domain underlie its gating. International journal

    Minako Hirano, Yuko Takeuchi, Takaaki Aoki, Toshio Yanagida, Toru Ide

    The Journal of biological chemistry   285 ( 6 )   3777 - 83   2010.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1074/jbc.M109.084368

    Web of Science

    PubMed

    researchmap

  • Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection.

    Takaaki Aoki, Minako Hirano, Yuko Takeuchi, Toshihide Kobayashi, Toshio Yanagida, Toru Ide

    Proceedings of the Japan Academy. Series B, Physical and biological sciences   86 ( 9 )   920 - 5   2010

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.2183/pjab.86.920

    Web of Science

    PubMed

    researchmap

  • 2P323 Mechanical control of single ion channel activity by Atomic Force Microscopy(The 48th Annual Meeting of the Biophysical Society of Japan)

    Kitta Mitsunori, Ide Toru, Hirano Minako, Takeuchi Yuko, Aoki Takaaki, Tanaka Hiroyuki, Yanagida Toshio, Kawai Tomoji

    Seibutsu Butsuri   50 ( 2 )   S139   2010

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.50.S139_4

    CiNii Article

    researchmap

  • GPHR is a novel anion channel critical for acidification of the Golgi apparatus

    Yusuke Maeda, Toru Ide, Masato Koike, Yasuo Uchiyama, Taroh Kinoshita

    JOURNAL OF PHARMACOLOGICAL SCIENCES   112   42P - 42P   2010

    　More details

    Language：English  

    Web of Science

    researchmap

  • A Polysaccharide-Based Container Transportation System Powered by Molecular Motors

    Youichi Tsuchiya, Tomotaka Komori, Minako Hirano, Tomohiro Shiraki, Akira Kakugo, Toru Ide, Jian-Ping Gong, Sunao Yamada, Toshio Yanagida, Seiji Shinkai

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   49 ( 4 )   724 - 727   2010

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/anie.200904909

    Web of Science

    researchmap

  • 3P236 The role of the cytoplasimic domain in pH-dependent gating by the KcsA channel(Biol & Artifi memb.: Excitation & Channels,The 48th Annual Meeting of the Biophysical Society of Japan)

    Hirano Minako, Ide Toru

    Seibutsu Butsuri   50 ( 2 )   S186 - S187   2010

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.50.S186_5

    CiNii Article

    researchmap

  • 3P235 Amphotericin B dimers form rectifying channels(Biol & Artifi memb.: Excitation & Channels,The 48th Annual Meeting of the Biophysical Society of Japan)

    Matsumori Nobuaki, Takeuchi Yuko, Aoki Takaaki, Hirano Minako, Ide Toru

    Seibutsu Butsuri   50 ( 2 )   S186   2010

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.50.S186_4

    CiNii Article

    researchmap

  • 3P240 Simultaneous optical and electrical recording of single DNA bindings to single hemolysin channels(Biol & Artifi memb.: Excitation & Channels,The 48th Annual Meeting of the Biophysical Society of Japan)

    Ide Toru, Hirano Minako

    Seibutsu Butsuri   50 ( 2 )   S187   2010

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.50.S187_4

    CiNii Article

    researchmap

  • Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane Reviewed

    Kazuhito V. Tabata, Ken Sato, Toru Ide, Takayuki Nishizaka, Akihiko Nakano, Hiroyuki Noji

    EMBO JOURNAL   28 ( 21 )   3279 - 3289   2009.11

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/emboj.2009.269

    Web of Science

    PubMed

    researchmap

  • Current recordings of ion channel proteins immobilized on resin beads. International journal

    Minako Hirano, Yuko Takeuchi, Takaaki Aoki, Toshio Yanagida, Toru Ide

    Analytical chemistry   81 ( 8 )   3151 - 4   2009.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/ac900286z

    Web of Science

    PubMed

    researchmap

  • 1P-191 Rearrangements in the KcsA cytoplasmic domain underlie its gating(Biol & Artifi memb.:Excitation & Channels, The 47th Annual Meeting of the Biophysical Society of Japan)

    Hirano Minako, Takeuchi Yuko, Aoki Takaaki, Yanagida Toshio, Ide Toru

    Seibutsu Butsuri   49   S92   2009

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.49.S92_4

    CiNii Article

    researchmap

  • CONFORMATIONAL CHANGES IN KcsA CHANNEL UPON pH-DEPENDENT GATING

    Hirano Minako, Yuko Takeuchi, Takaaki Aoki, Toshio Yanagida, Toru Ide

    JOURNAL OF PHYSIOLOGICAL SCIENCES   59   256 - 256   2009

    　More details

    Language：English  

    Web of Science

    researchmap

  • 2P-181 Single antibiotics channel current measurement and its application for biosensor(Biol & Artifi memb.:Excitation & Channels,The 47th Annual Meeting of the Biophysical Society of Japan)

    Aoki Takaaki, Hirano Minako, Takeuchi Yuko, Kobayashi Toshihide, Yanagida Toshio, Ide Toru

    Seibutsu Butsuri   49   S135   2009

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.49.S135_2

    CiNii Article

    researchmap

  • 2P-180 Gating mechanism analysis in voltage sensitive potassium channels(Biol & Artifi memb.:Excitation & Channels,The 47th Annual Meeting of the Biophysical Society of Japan)

    Takeuchi Yuko, Hirano Minako, Aoki Takaaki, Yanagida Toshio, Ide Toru

    Seibutsu Butsuri   49   S135   2009

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.49.S135_1

    CiNii Article

    researchmap

  • Lipid bilayers at the gel interface for single ion channel recordings. International journal

    Toru Ide, Toshihide Kobayashi, Minako Hirano

    Analytical chemistry   80 ( 20 )   7792 - 5   2008.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/ac801224a

    Web of Science

    PubMed

    researchmap

  • GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus. International journal

    Yusuke Maeda, Toru Ide, Masato Koike, Yasuo Uchiyama, Taroh Kinoshita

    Nature cell biology   10 ( 10 )   1135 - 45   2008.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/ncb1773

    Web of Science

    PubMed

    researchmap

  • Cy3-3-acylcholine: a fluorescent analogue of acetylcholine for single molecule detection. International journal

    Kenzo Fujimoto, Yoshinaga Yoshimura, Makoto Ihara, Kazuhiko Matsuda, Yuko Takeuchi, Takaaki Aoki, Toru Ide

    Bioorganic & medicinal chemistry letters   18 ( 3 )   1106 - 9   2008.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bmcl.2007.12.002

    Web of Science

    PubMed

    researchmap

  • 3P-203 Lipid Bilayers at the Gel Interface for single Ion Channel Recordings(The 46th Annual Meeting of the Biophysical Society of Japan)

    Ide Toru, Hirano Minako

    Seibutsu Butsuri   48   S159   2008

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.48.S159_1

    CiNii Article

    researchmap

  • Lipid bilayers at gel/gel interface for ion channel recordings

    Hirano, M., Kobayashi, T., Ide, T.

    e-Journal of Surface Science and Nanotechnology   6   130 - 133   2008

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1380/ejssnt.2008.130

    Web of Science

    Scopus

    CiNii Article

    researchmap

  • 3P-222 Lysenin channel as a nanopore for biosensing applications(The 46th Annual Meeting of the Biophysical Society of Japan)

    Aoki Takaaki, Hirano Minako, Takeuchi Yuko, Kobayashi Toshihide, Yanagida Toshio, Ide Toru

    Seibutsu Butsuri   48   S161 - S162   2008

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.48.S161_7

    CiNii Article

    researchmap

  • 3P-207 Simultaneous measurements of optical and electrical properties of potassium channel, KcsA(The 46th Annual Meeting of the Biophysical Society of Japan)

    Hirano Minako, Takeuchi Yuko, Aoki Takaaki, Yanagida Yoshio, Ide Toru

    Seibutsu Butsuri   48   S159   2008

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.48.S159_5

    CiNii Article

    researchmap

  • 3P-196 Cell-free synthesis and reconstitution of BK channels into planar lipid bilayers(The 46th Annual Meeting of the Biophysical Society of Japan)

    Takeuchi Yuko, Aoki Takaaki, Hirano Minako, Ide Toru

    Seibutsu Butsuri   48   S157 - S158   2008

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.48.S157_6

    CiNii Article

    researchmap

  • 2P296 Single channel properties of lysenin measured in the artificial lipid bilayer. II : effect of lipid composition and poly-L-lysin(Native and artificial biomembranes-excitation and channels,Poster Presentations)

    Aoki Takaaki, Hirano Minako, Takeuchi Yuko, Kobayashi Toshihide, Yanagida Toshio, Ide Toru

    Seibutsu Butsuri   47   S187   2007

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.47.S187_1

    CiNii Article

    researchmap

  • Immobilizing BK-channels in artificial lipid bilayers using annexin V

    Yuko Takeuchi, Takaaki Aoki, Toshio Yanagida, Toru Ide

    E-JOURNAL OF SURFACE SCIENCE AND NANOTECHNOLOGY   5   1 - 5   2007

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1380/ejssnt.2007.1

    Web of Science

    researchmap

  • 2P294 Simultaneous measurements of optical and electrical properties of the Ca^<2+> - activated potassium channel, MthK(Native and artificial biomembranes-excitation and channels,Poster Presentations)

    Hirano Minako, Takeuchi Yuko, Aoki Takaaki, Yanagida Toshio, Ide Toru

    Seibutsu Butsuri   47   S186   2007

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.47.S186_3

    CiNii Article

    researchmap

  • 2P293 SImultaneous optical and electrical measurement of single channel-drug interaction(Native and artificial biomembranes-excitation and channels,Poster Presentations)

    Ide Toru, Hirano Minako, Aoki Takaaki, Takeuchi Yuko

    Seibutsu Butsuri   47   S186   2007

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.47.S186_2

    CiNii Article

    researchmap

  • 2P295 Proton Current Measurement of the Stator Complex MotA/MotB of the Flagellar Motor Expressed in Xenopus Oocytes(Native and artificial biomembranes-excitation and channels,Poster Presentations)

    Che Yong-Suk, Takeuchi Yuko, Minamino Tohru, Ide Toru, Namba Keiichi

    Seibutsu Butsuri   47   S186   2007

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.47.S186_4

    CiNii Article

    researchmap

  • Lysenin forms a voltage-dependent channel in artificial lipid bilayer membranes. International journal

    Toru Ide, Takaaki Aoki, Yuko Takeuchi, Toshio Yanagida

    Biochemical and biophysical research communications   346 ( 1 )   288 - 92   2006.7

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Lysenin, a hemolytic protein derived from the body fluid of earthworm, was incorporated into artificial bilayer membranes. Upon insertion, it formed a voltage-dependent large conductance channel in asolectin bilayers in a sphingomyelin-dependent manner. The channel had low ion-selectivity. Single-channel conductance was calculated as approximately 550 pS in 100 mM KCl. The channel in asolectin bilayers closed when the membrane was held at a positive potential. In contrast, the channel showed no voltage dependency in membranes made of pure phosphatidylcholine and sphingomyelin, suggesting some lipid contents included in the asolectin membranes affected channel gating.

    PubMed

    researchmap

  • Immobilizing single lipid and channel molecules in artificial lipid bilayers with annexin A5. International journal

    Takehiko Ichikawa, Takaaki Aoki, Yuko Takeuchi, Toshio Yanagida, Toru Ide

    Langmuir : the ACS journal of surfaces and colloids   22 ( 14 )   6302 - 7   2006.7

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/la0535025

    Web of Science

    PubMed

    researchmap

  • 1P360 Imaging of COPII vesicle formation and budding from artificial membrane(13. Membrane transport,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Tabata Kazuhito, Sato Ken, Ide Toru, Noji Hiroyuki

    Seibutsu Butsuri   46 ( 2 )   S236   2006

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.46.S236_4

    CiNii Article

    researchmap

  • 1P372 Utilization of synthetic fluorescent agonist of nAChR for simultaneous optical and electrical single molecule measurements(14. Ion channels and receptors,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Takeuchi Yuko, Aoki Takaaki, Ihara Makoto, Yoshimura Yoshinaga, Fujimoto Kenzo, Matsuda Kazuhiko, Ide Toru

    Seibutsu Butsuri   46 ( 2 )   S239   2006

    　More details

    Language：English   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.46.S239_4

    CiNii Article

    researchmap

  • A novel method for artificial lipid-bilayer formation. International journal

    Toru Ide, Takehiko Ichikawa

    Biosensors & bioelectronics   21 ( 4 )   672 - 7   2005.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Many proposals have been made regarding the development of biosensors using single-channel recording with an artificial planar bilayer. The fragile nature of bilayer membranes is the major difficulty for the application of the artificial bilayer technique to the development of biosensors. We have developed an apparatus that promptly forms artificial bilayers. This technique is more efficient than other techniques for forming artificial bilayers. Bilayer membranes could be formed within 10s requiring 1 microl of analyte solution to record single-channel currents using our apparatus. A bilayer was formed by pressing the membrane on an agarose layer with hydraulic pressure. With this novel apparatus, we have recorded single-channel currents of various types of channels such as the BK-channel, the nicotinic receptor channel and the ryanodine receptor channel. The properties of the channels determined with this novel technique agreed well with those determined with conventional techniques.

    PubMed

    researchmap

  • An artificial lipid bilayer formed on a PEG-coated glass for simultaneous electrical and optical measurement of single ion-channels

    Toru Ide, Yuko Takeuchi, Takaaki Aoki, Kazuhito Tabata, Hiroyuki Noji

    e-Journal of Surface Science and Nanotechnology   3   70 - 73   2005

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1380/ejssnt.2005.70

    Web of Science

    researchmap

  • Annexin 5 decreases the diffusion of lipid and channel molecules in an artificial lipid bilayer

    Takehiko Ichikawa, Yuko Takeuchi, Takaaki Aoki, Toru Ide

    E-JOURNAL OF SURFACE SCIENCE AND NANOTECHNOLOGY   3   213 - 217   2005

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1380/ejssnt.2005.213

    Web of Science

    researchmap

  • Non-contact surface force microscopy for molecular interaction study

    Takaaki Aoki, Yoshiyuki Sowa, Toru Ide, Toshio Yanagida

    E-JOURNAL OF SURFACE SCIENCE AND NANOTECHNOLOGY   3   46 - 50   2005

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1380/ejssnt.2005.46

    Web of Science

    researchmap

  • 1P150 Development of the single molecule imaging system of the F_o motor

    Ueno H., Tabata K., Suzuki T., Iino R., Ide T., Yoshida M., Noji H.

    Seibutsu Butsuri   45   S69   2005

    　More details

    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.45.S69_2

    CiNii Article

    researchmap

  • An amphotericin B-ergosterol covalent conjugate with powerful membrane permeabilizing activity. International journal

    Nobuaki Matsumori, Noritsugu Eiraku, Shigeru Matsuoka, Tohru Oishi, Michio Murata, Takaaki Aoki, Toru Ide

    Chemistry & biology   11 ( 5 )   673 - 9   2004.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Amphotericin B-sterol conjugates were synthesized and examined for their membrane permeabilizing activity. Ergosterol and cholesterol, each connected with amphotericin B via an ethylenecarbamate or hexamethylenecarbamate linker, were examined by K(+) flux assays using liposomes and by single-channel recording across phospholipid membrane. Among four conjugates tested, AmB-ergosterol bearing an ethylenecarbamate linker exhibited the most powerful activity, which substantially exceeded that of the cholesterol homolog. Single-channel recording clearly exhibited that the ergosterol conjugate elicited channel current with the conductance of 28 pS, which was comparable with those by AmB, and revealed a higher channel open probability than the cholesterol conjugate. These results imply that direct interaction between amphotericin B and ergosterol is reproduced by their conjugate, which may serve as a model compound for understanding the drug's selective toxicity.

    PubMed

    researchmap

  • 1P230 Monitoring single protein in artificial lipid bilayers which diffusion is decreased by annexin5

    Ichikawa T., Ide T., Yanagida T.

    Seibutsu Butsuri   44   S87   2004

    　More details

    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.44.S87_2

    CiNii Article

    researchmap

  • Ion channel assemblage formed by Amphotericin B derivatives in biological membranes : single channel current recording

    Aoki T., Takeuchi Y., Matsuoka S., Eiraku N., Matsumori N., Ide T., Murata M.

    Seibutsu Butsuri   43   S177   2003

    　More details

    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.43.S177_3

    CiNii Article

    researchmap

  • Simultaneous optical and electrical recording of a single ion-channel.

    Toru Ide, Yuko Takeuchi, Takaaki Aoki, Toshio Yanagida

    The Japanese journal of physiology   52 ( 5 )   429 - 34   2002.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    In recent years, the single-molecule imaging technique has proven to be a valuable tool in solving many basic problems in biophysics. The technique used to measure single-molecule functions was initially developed to study electrophysiological properties of channel proteins. However, the technology to visualize single channels at work has not received as much attention. In this study, we have for the first time, simultaneously measured the optical and electrical properties of single-channel proteins. The large conductance calcium-activated potassium channel (BK-channel) labeled with fluorescent dye molecules was incorporated into a planar bilayer membrane and the fluorescent image captured with a total internal reflection fluorescence microscope simultaneously with single-channel current recording. This innovative technology will greatly advance the study of channel proteins as well as signal transduction processes that involve ion permeation processes.

    PubMed

    researchmap

  • pH-Dependent Fusion of Synaptosomal Membrane Studied by Fluorescence Quenching Method.

    KUMAMARU Emi, SATO Masayuki, YOSHIDA Hiroko, IDE Toru, KASAI Michiki

    The Journal of Physiological Sciences   49 ( 1 )   19 - 25   1999

    　More details

    Language：English   Publisher：PHYSIOLOGICAL SOCIETY OF JAPAN  

    DOI： 10.2170/jjphysiol.49.19

    CiNii Article

    researchmap

  • A voltage- and K+-dependent K+ channel from a membrane fraction enriched in contractile vacuole of Dictyostelium discoideum. Reviewed

    Yoshida, K, Ide, T, Inouye, K, Mizuno, K, Taguchi, T, Kasai, M

    Biochimica et Biophysica Acta - Biomembranes   1325 ( 2 )   178 - 188   1997.2

    　More details

    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    researchmap

  • ATP-sensitive anion channel from rat brain synaptosomal membranes incorporated into planar lipid bilayers. Reviewed

    Yuto, J, Ide, T, Kasai, M

    Biophysical Journal   72 ( 21 )   720 - 727   1997.2

    　More details

    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    CiNii Article

    researchmap

  • Calsequestrin is essential for the Ca2+ release induced by myotoxin α in skeletal muscle sarcoplasmic reticulum. Reviewed

    Ohkura, M, Ide, T, Furukawa, K.-I, Kawasai, T, Kasai, M, Ohizumi, Y

    Canadian Journal of Physiology and Pharmacology   73 ( 8 )   1181 - 1185   1995.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    researchmap

  • An anion channel from transverse tubular membranes incorporated into planar bilayers. Reviewed

    Ide, T, Hidaka, J, Kasai, M

    Biochimica et Biophysica Acta - Biomembranes   1237 ( 2 )   115 - 120   1993.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    researchmap

  • Identification of an anion channel protein from transverse tubules of rabbit skeletal muscle. Reviewed

    Hidaka, J, Ide, T, Taguchi, T, Kasai, M

    Annals of the New York Academy of Sciences   707   424 - 426   1993.12

    　More details

    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    researchmap

  • Purification of a K+ channel protein of sarcoplasmic reticulum by assaying the channel activity in the planar lipid bilayer system.

    Ide, T, Morita, T, Kawasaki, T, Taguchi, T, Kasai, M

    Biochimica et Biophysica Acta - Biomembranes   1067 ( 2 )   213 - 220   1991.8

    　More details

    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    CiNii Article

    researchmap

  • Purification of a Cl--channel protein of sarcoplasmic reticulum by assaying the channel activity in the planar lipid bilayer system. Reviewed

    Ide, T, Sakamoto, H, Morita, T, Taguchi, T, Kasai, M

    Biochemical and Biophysical Research Communications, 176 (1), pp. 38-44   176 ( 1 )   38 - 44   1991.4

    　More details

    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    researchmap

  • Mast cell degranulating peptide forms voltage gated and cation-selective channels in lipid bilayers. Reviewed

    Ide, T, Taguchi, T, Morita, T, Sato, M, Ikenaka, K, Aimoto, S, Kondo, T, Hojo, H, Kasai, M, Mikoshiba, K

    Biochemical and Biophysical Research Communications   163 ( 1 )   155 - 160   1989.8

    　More details

    Authorship：Lead author  

    researchmap

▼display all

MISC

  • 非常に強い殺蚊活性を示すCry11Baトキシンの作用機構

    新谷彩子, 白石優里, 汐崎友哉, 井出徹, 早川徹

    日本分子生物学会年会プログラム・要旨集(Web)   42nd   2019

    　More details

  • 殺蚊トキシンCry4Aaが形成するチャネルポアの性状

    白石優里, 小薗寛人, 宮崎美登香, 新谷彩子, 井出徹, 早川徹

    日本分子生物学会年会プログラム・要旨集(Web)   42nd   2019

    　More details

  • ハイブリッドフォトディテクタ(HPD)による広視野高時間分解能生体1分子蛍光検出

    深澤宏仁, 中野学, 長澤貴康, 平野美奈子, 井出徹, 横田浩章

    レーザ顕微鏡研究会講演会抄録集   44th   2019

    　More details

  • 殺蚊トキシンCry46Abの小孔形成と殺虫活性

    早川徹, 宮崎美登香, 榊原暁, 原田翔也, 朝倉真実, 井出徹

    日本分子生物学会年会プログラム・要旨集(Web)   42nd   2019

    　More details

  • 新規な殺蚊Cry46Abトキシンの殺虫活性メカニズム

    早川徹, 榊原暁, 武部聡, 井出徹

    日本応用動物昆虫学会大会講演要旨   63rd   2019

    　More details

  • PTEN-PI (4, 5)P2 positive feedback mechanism for stabilizing asymmetric PI (3, 4, 5)P3 localization in migrating cell

    YOSHIOKA Daisuke, YOSHIOKA Daisuke, KOTEISHI Hiroyasu, OKUNO Daichi, MATSUOKA Satomi, IDE Toru, UEDA Masahiro, UEDA Masahiro

    生物物理(Web)   58 ( Supplement 1-2 )   2018

    　More details

  • ペプチドタグ4AaCterを利用したCryトキシンの製剤化

    早川徹, 岡崎友亮, 井出徹

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集   87th   2017

    　More details

  • In vitro1分子イメージングによるPTEN-PI(4,5)P2相互作用の解析

    好岡大輔, 福島誠也, 福島誠也, 奥野大地, 松岡里実, 井出徹, 上田昌宏, 上田昌宏

    日本細胞生物学会大会(Web)   69th   2017

    　More details

  • 殺蚊Cryトキシンが示す殺虫活性助長作用

    早川徹, 迫田陽子, 米田直也, 井出徹

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集   86th   2016

    　More details

  • 1分子計測法によるチャネル蛋白ダイナミクスの研究

    井出徹

    倉田奨励金研究報告   46   2016

    　More details

  • A mechanism of asymmetric PTEN distribution revealed by in vitro single-molecule imaging analysis

    YOSHIOKA Daisuke, FUKUSHIMA Seiya, FUKUSHIMA Seiya, KOTEISHI Hiroyasu, OKUNO Daichi, MATSUOKA Satomi, IDE Toru, UEDA Masahiro, UEDA Masahiro

    日本細胞生物学会大会(Web)   68th   2016

    　More details

  • Phosphatidylinositol lipid PI(4,5)P2 enhances membrane binding of PTEN revealed by single-molecule imaging on artificial lipid bilayers

    YOSHIOKA Daisuke, FUKUSHIMA Seiya, FUKUSHIMA Seiya, KOTEISHI Hiroyasu, OKUNO Daichi, MATSUOKA Satomi, IDE Toru, UEDA Masahiro, UEDA Masahiro

    日本蛋白質科学会年会プログラム・要旨集   16th   2016

    　More details

  • Development of the Line Confocal System for the Single Molecule Tracking of Fast Folding Dynamics of Proteins

    Hiroyuki Oikawa, Kiyoto Kamagata, Munehito Arai, Atsuhito Fukasawa, Hiroaki Yokota, Toru Ide, Satoshi Takahashi

    BIOPHYSICAL JOURNAL   108 ( 2 )   50A - 51A   2015.1

    　More details

    Language：English   Publishing type：Research paper, summary (international conference)  

    DOI： 10.1016/j.bpj.2014.11.309

    Web of Science

    researchmap

  • 殺蚊トキシン由来のペプチドタグ4AaCterによるタンパク質凝集体形成機構

    早川徹, 岡崎友亮, 中西里菜, 永尾権, 佐藤友美, 井出徹

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集   85th   2015

    　More details

  • マイクロ秒分解一分子FRET測定よるタンパク質折り畳みダイナミクスの追跡

    小井川浩之, 新井宗仁, 深澤宏仁, 深澤宏仁, 横田浩章, 井出徹, 高橋聡

    分子科学討論会講演プログラム&要旨(Web)   9th   2015

    　More details

  • イオンチャネルの1分子計測および操作

    井出徹

    日本生化学会大会(Web)   88th   2015

    　More details

  • Bt菌が産生するCryトキシンを用いて蚊の効率的な防除法を開発する試み

    早川徹, 早川徹, 迫田陽子, 米田直也, 井出徹, 井出徹

    日本生化学会大会(Web)   88th   2015

    　More details

  • チャネル蛋白質の1分子計測

    井出徹

    キャピラリー電気泳動シンポジウム講演要旨集   35th   2015

    　More details

  • 無細胞タンパク質発現/リポソーム系を用いた1段階KcsA組込みリポソームの構築と機能解析

    安藤満, 安藤満, 秋山源, 奥野大地, 平野美奈子, 井出徹, 澤田晋一, 澤田晋一, 佐々木善浩, 秋吉一成, 秋吉一成

    日本膜学会年会講演要旨集   37th   2015

    　More details

  • 改良したマイクロ秒分解一分子蛍光測定法による蛋白質の高速折り畳みダイナミクスの追跡

    小井川浩之, 鎌形清人, 新井宗仁, 深澤宏仁, 深澤宏仁, 横田浩章, 井出徹, 高橋聡

    日本蛋白質科学会年会プログラム・要旨集   14th   2014

    　More details

  • Bt菌が産生する殺蚊トキシンCry4Aaの複雑な作用機構について

    早川徹, HOWLADER Mohammad Tofazzal H., 中尾早織, 井出徹

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集   84th   2014

    　More details

  • 大腸菌を用いて殺蚊トキシンCry11Aaを効率的に生産する方法

    早川徹, 佐野乙香, 井出徹

    日本分子生物学会年会プログラム・要旨集(Web)   36th   2013

    　More details

  • 明らかになった葉緑体のトランスロコン

    菊地真吾, 平野美奈子, 井出徹, 中井正人

    細胞工学   32 ( 8 )   2013

    　More details

  • 人工細胞膜を用いたイオンチャネル創薬スクリーニングシステム

    川野竜司, 辻祐太郎, 辻祐太郎, 神谷厚輝, 大崎寿久, 平野美奈子, 平野美奈子, 井出徹, 井出徹, 三木典尚, 三木典尚, 竹内昌治, 竹内昌治

    高分子学会予稿集(CD-ROM)   61 ( 2 )   2012

    　More details

  • Visualization of cargo dynamics in COPII vesicle formation on artificial planar lipid membrane

    Kazuhito V. Tabata, Ken Sato, Toru Ide, Takayuki Nishizaka, Akihiko Nakano, Hiroyuki Noji

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS   1797   45 - 46   2010.7

    　More details

    Language：English   Publishing type：Research paper, summary (international conference)  

    DOI： 10.1016/j.bbabio.2010.04.153

    Web of Science

    researchmap

  • AFM操作による単一分子カリウムチャンネルの開閉

    橘田晃宜, 平野美奈子, 柳田敏雄, 田中裕行, 田中裕行, 井出徹, 川合知二

    表面科学学術講演会講演要旨集   30th   2010

    　More details

  • 可視1分子計測によるチャネルたんぱく質の分子内運動計測

    井出徹, 井出徹, 平野美奈子, 平野美奈子, 橘田晃宜

    表面科学学術講演会講演要旨集   30th   2010

    　More details

  • 微小プローブによるチャネルタンパクの直接操作

    井出徹, 平野美奈子, 橘田晃宜

    日本生体エネルギー研究会討論会講演要旨集   36th   2010

    　More details

  • KcsAチャネルの細胞内ドメインによるプロトン感受機構の解明

    平野美奈子, 井出徹

    日本生体エネルギー研究会討論会講演要旨集   36th   2010

    　More details

  • 多糖を基体とした分子モーター駆動の人工コンテナ輸送システム

    土屋陽一, 小森智貴, 平野美奈子, 白木智丈, 角五彰, 井出徹, GONG Jianping, 山田淳, 柳田敏雄, 新海征治

    日本化学会講演予稿集   90th ( 3 )   2010

    　More details

  • 膜タンパク質の電気的・力学的同時計測による一分子測定

    橘田晃宜, 平野美奈子, 柳田敏雄, 田中裕行, 田中裕行, 井出徹, 川合知二

    応用物理学会学術講演会講演予稿集(CD-ROM)   71st   2010

    　More details

  • Kチャネルゲートの1分子操作

    井出徹

    日本蛋白質科学会年会プログラム・要旨集   10th   2010

    　More details

  • COP II小胞形成タンパクによる輸送基質の濃縮と輸送されない蛋白質の排除機構

    田端和仁, 佐藤健, 井出徹, 西坂崇之, 中野明彦, 野地博行

    日本蛋白質科学会年会プログラム・要旨集   9th   2009

    　More details

  • GPHRはゴルジ装置の酸性化・機能にとって重要な新規アニオンチャネルである

    前田裕輔, 井出徹, 小池正人, 内山安男, 木下タロウ

    解剖学雑誌   84 ( Supplement )   2009

    　More details

  • 人工脂質二重膜上に再構成したCOPIIタンパクによる輸送基質の濃縮と輸送されないタンパク質の排除

    田端和仁, 佐藤健, 井出徹, 西坂崇之, 中野明彦, 野地博行

    生化学   2009

    　More details

  • COPII小胞形成過程における輸送基質の濃縮と排除

    田端和仁, 佐藤健, 井出徹, 西坂崇之, 中野明彦, 野地博行

    日本生体エネルギー研究会討論会講演要旨集   35th   2009

    　More details

  • COP II小胞形成タンパクによる輸送基質の濃縮と輸送されない蛋白質の排除機構

    田端和仁, 佐藤健, 井出徹, 西坂崇之, 中野明彦, 野地博行

    日本蛋白質科学会年会プログラム・要旨集   9th   2009

    　More details

  • 世界と共に発展するための中核技術-美と技の創造物 ハイドロゲル界面に形成した脂質二分子膜-1分子センサーの開発を目指して-

    井出徹

    Materials Integration   21 ( 8 )   2008

    　More details

  • 膜タンパクの電気・光学的-分子計測

    井出徹

    表面科学講演大会講演要旨集   27th   2007

    　More details

  • 人工脂質二重膜を用いたCOPII小胞形成のイメージング

    田端和仁, 井出徹, 野地博行, 佐藤健, 中野明彦

    電気学会バイオ・マイクロシステム研究会資料   BMS-06 ( 15-21 )   2006

    　More details

  • 機能性イオンチャネル

    井出徹

    ソフト・オプトエレクトロニクス材料4-情報を共有する分子システム 理研シンポジウム/第154回JOEM講演要旨集 平成18年   2006

    　More details

  • 1P528 Development of the Single Molecule Imaging System of the F_0 Motor(26. Single molecule biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Ueno Hiroshi, Tabata Kazuhito, Suzuki Toshiharu, Ide Toru, Yoshida Masasuke, Noji Hiroyuki

    Seibutsu Butsuri   46 ( 2 )   S278   2006

    　More details

    Language：English   Publisher：一般社団法人 日本生物物理学会  

    DOI： 10.2142/biophys.46.S278_4

    CiNii Article

    researchmap

  • 単一チャネルの電気・光学的同時計測

    井出徹, 青木高明, 竹内裕子

    生物物理   45 ( 3 )   2005

    　More details

  • Optical and electrical simultaneous measurement of ion channel monomolecular using artificial lipid membrane.

    竹内裕子, 市川壮彦, 青木高明, 井出徹

    バイオイメージング   14 ( 4 )   2005

    　More details

  • 1P151 Development and verification of the observation system for F_oF_1-ATP synthase rotation driven by membrane potential

    Tabata K, Iino R, Ueno H, Yamada-Kato Y, Ide T, Noji H

    Seibutsu Butsuri   45 ( 0 )   S69   2005

    　More details

    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.45.S69_3

    CiNii Article

    researchmap

  • アネキシン5で流動性を減少させた人工脂質三重膜中の1分子観測

    市川壮彦, 井出徹, 柳田敏雄

    日本分子生物学会年会プログラム・講演要旨集   27th   2004

    　More details

  • 生理学実験法講座「1分子生理学」 「単一チャネルの電気・光学的同時計測」

    井出徹, 青木高明, 竹内裕子

    日本生理学雑誌   65 ( 9 )   2003

    　More details

  • Tip-Dip人工膜法を用いたチャネル蛋白の1分子イメージング

    青木高明, 竹内裕子, 井出徹, 柳田敏雄

    日本生物物理学会年会講演予稿集   40th   2002

    　More details

  • イオンチャネル 分子の光学的,電気的同時計測

    竹内裕子, 井出徹, 柳田敏雄

    日本神経科学大会プログラム・抄録集   25th   2002

    　More details

  • 単一チャネルの電気・光学的同時計測

    井出徹, 竹内裕子, 青木高明, 柳田敏雄

    日本生物物理学会年会講演予稿集   40th   2002

    　More details

  • 心筋型リアノジン受容チャネル1分子の光学的,電気的同時計測

    竹内裕子, 井出徹, 青木高明, 柳田敏雄

    日本生物物理学会年会講演予稿集   40th   2002

    　More details

  • 単一イオンチャネルの電気・光学的同時計測系の開発

    井出徹, 竹内裕子, 柳田敏雄

    日本生物物理学会年会講演予稿集   39th   2001

    　More details

  • イオンチャネルの1分子計測

    井出徹, 坂本裕人, 柳田敏雄

    生化学   72 ( 8 )   2000

    　More details

  • 単一チャネルタンパク質の電気的・光学的同時計技術の開発

    井出徹

    生理学研究所年報   20   1999

    　More details

  • Optical and electrical measurement of artificial planar lipid membranes

    IDE T., KASAI M.

    Biophysics   37   S50   1997.10

    　More details

    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    CiNii Article

    CiNii Books

    J-GLOBAL

    researchmap

  • 収縮胞K・チャネル 透過性イオンによるブロック

    吉田邦人, 井出徹, 井上敬, 水野孝一, 田口隆久, 葛西道生

    日本生物物理学会年会講演予稿集   34th   1996

    　More details

  • ラット大脳シナプス膜ATP感受性陰イオンチャネルの解析

    井出徹, 湯藤潤, 葛西道生

    日本生物物理学会年会講演予稿集   34th   1996

    　More details

  • Research on molecular mechanism of calcium channel switching of skeletal muscle sarcoplasmic reticulum. ( Ministry of Education S )

    葛西道生, 井出徹, 川崎隆史, 山口直宏

    イオンチャネルにおける動的構造機能連関の先駆的研究 平成7年度 No.05304055   1996

    　More details

  • Regulation of cation channels by a luminal Ca2+-binding protein in sarcoplasmic reticulum.

    坂本裕人, 井出徹, 葛西道生

    日本生物物理学会年会講演予稿集   33rd   1995

    　More details

  • A rectifying K+ channel from Dictyostelium discoideum.

    吉田邦人, 井出徹, 井上敬, 田口隆久, 葛西道生

    日本生物物理学会年会講演予稿集   33rd   1995

    　More details

  • Electrophysiological study on an anion channel of rabbit skeletal T-tubular membrane.

    井出徹, 日高淳, 葛西道生

    日本生物物理学会年会講演予稿集   32nd   1994

    　More details

  • Regulation of calcium channel in sarcoplasmic reticulum by calsequestrin.

    葛西道生, 山口直宏, 川崎隆史, 井出徹

    日本生物物理学会年会講演予稿集   32nd   1994

    　More details

  • Electrophysiological study on an anion channel study of rat synaptsomal membrane.

    湯藤潤, 井出徹, 葛西道生

    日本生物物理学会年会講演予稿集   32nd   1994

    　More details

  • Study of ion channels incorporated into planar lipid bilayers from the plasma membrane of Dictyostelium.

    吉田邦人, 井出徹, 井上敬, 葛西道生

    日本生物物理学会年会講演予稿集   32nd   1994

    　More details

  • Fluorescence measurement of Cl- influx into sarcoplasmic reticulum vesicles.

    井出徹, 葛西道生

    日本生物物理学会年会講演予稿集   31st   1993

    　More details

  • Study of unknown glycoprotein in rabbit skeltal muscle sarcoplasmic reticulum.

    坂本裕人, 井出徹, 葛西道生

    日本生物物理学会年会講演予稿集   31st   1993

    　More details

  • ウサギ骨格筋T管膜に存在するCl-チャンネルの機能解析

    日高淳, 川崎隆史, 井出徹, 田口隆久, 葛西道生

    日本生物物理学会年会講演予稿集   30th   1992

    　More details

▼display all

Presentations

  • Btトキシン小孔の電気生理学的解析に役立つ脂質二重膜形成法

    奥田 翼, 竹内 智哉, 朝倉 真実, 平野 美奈子, 井出 徹, 早川 徹

    第47回日本分子生物学会年会（福岡，2024/11/27-29） 

    　More details

    Event date： 2024.11.27 - 2024.11.29

    Language：Japanese  

    researchmap

  • Regulatory mechanism of photoactivated adenylyl cyclase activity

    Hinase Kondo, Masahiko Taguchi, Syun Sakuraba, Masumi Takebe, Koki Shimomae, Mei Kawamoto, Mami Asakura, Akiya Hukuda, Eriko Nango, Hidetoshi Kono, Toru Ide, Minako Hirano

    The 63rd Annual Meeting of the Biophysical Society of Japan  2025.9 

    　More details

  • Development of a system to measure activity of photoactivated adenylyl cyclase using cyclic nucleotide-gated channels

    Shunsuke Kiguchi, Kosei Higuchi, Koshiro Morita, Mami Asakura, Toru Ide, Minako Hirano

    The 63rd Annual Meeting of the Biophysical Society of Japan  2025.9 

    　More details

  • The role of the C-terminal region in regulating the activity of the photoactivated adenylyl cyclase from Oscillatoria acuminata

    Reito Yasui, Masahiko Taguchi, Syun Sakuraba, Masumi Takebe, Saiko Akizuki, Mami Asakura, Akiya Hukuda, Eriko Nango, Hidetoshi Kono, Toru Ide, Minako Hirano

    2025.9 

    　More details

  • Development of a simple method for the reconstitution of ion channels using agarose gel beads

    Mami Asakura, Kohta Takagi, Miho Ohnishi, Minako Hirano, Toru Ide

    The 63rd Annual Meeting of the Biophysical Society of Japan  2025.9 

    　More details

  • 異なる殺虫トキシン(Mpp46AbとCry4Aa)を混合使用すると殺虫活性が助長される訳

    早川 徹, 山岡 瞬, 朝倉 真実, 井出 徹

    第47回日本分子生物学会年会（福岡，2024/11/27-29）  2024.11.27 

    　More details

  • Identification of the important region for photoactivity in photoactivated adenylyl cyclase

    Hirano M., Takebe M., Kondo H., Asakura M., Ide T.

    21st IUPAB Congress 2024 in collaboration with The 62nd Annual Meeting of the Biophysical Society of Japan, Kyoto (2024.6)  2024.6 

    　More details

    Language：English   Presentation type：Poster presentation  

    researchmap

  • Developmen of a high-throughpu device for recording channel currents using agarose gel bead

    Asakura M., Wang S., Hirano M., Ide T.

    21st IUPAB Congress 2024 in collaboration with The 62nd Annual Meeting of the Biophysical Society of Japan, Kyoto (2024.6)  2024.6 

    　More details

    Language：English   Presentation type：Poster presentation  

    researchmap

  • Development of a simple single-channel current measurement system

    Tomomi Murata, Toru Ide, Minako Hirano, Mami Asakura

    The 61th Annual Meeting of the Biophysical Society of Japan  2023.11 

    　More details

  • Identification of Key Amino Acids affecting the Activity of Photoactivated Adenylyl Cyclase from Oscillatoria acuminata

    Minako Hirano, Masumi Takebe, Syunshi Yano, Hinase Kondo, Ayu Yuasa, Toru Ide

    The 61th Annual Meeting of the Biophysical Society of Japan  2023.11 

    　More details

  • Development of channel current measurement device using agarose gel beads

    Mami Asakura, Shuyan Wang, Minako Hirano, Toru Ide

    The 61th Annual Meeting of the Biophysical Society of Japan  2023.11 

    　More details

  • Structural changes of adenylate cyclase from Oscillatoria acuminata in response to blue light stimulation

    Yuki Kitamura, Toru Ide, Minako Hirano

    The 60th Annual Meeting of the Biophysical Society of Japan  2022.9 

    　More details

  • Current status of low-light photodetectors capable of detecting near-infrared light

    Atsuhito Fukasawa, Minako Hirano, Toru Ide, Hiroaki Yokota

    2022.9 

    　More details

  • An artificial lipid bilayer ion-channel recording method using agarose gel beads,

    Mami Asakura, Atsuya Mukuno, Minako Hirano, Toru Ide

    The 60 th Annual Meeting of;he Biophysical;Society of Japan  2022.9 

    　More details

  • Development of an automated system for measuring channel currents using a gold probe

    Hirano, M, Tomita, M, Takahashi, C, Kawashima, N, Ide, T

    The 59th annual meeting of Biophysical Society of Japan  2021.11 

    　More details

  • Channel-pores formation of Bacillus thuringiensis Cry46Ab toxin and its mosquitocidal activity

    Midoka Miyazaki, Tohru Hayakawa, Toru Ide

    The 59th annual meeting of Biophysical Society of Japan  2021.11 

    　More details

    Language：English   Presentation type：Oral presentation (general)  

    researchmap

  • Artificial bilayers on hydrogel for channel current recordings

    Toru Ide, Minako Hirano, Daiki Yamamoto, Mami Asakura, Yuki Kitamura

    The 58th Annual Meeting of the Biophysical Society of Japan  2020.9 

    　More details

    Language：English   Presentation type：Oral presentation (general)  

    researchmap

  • Local ambient condition monitoring by hybrid photo-detector (HPD)-based wide-field single-molecule fluorescence detection

    Atsuhito Fukasawa, Gaku Nakano, Takayasu Nagasawa, Minako Hirano, Toru Ide, Hiroaki Yokota

    The 58h Annual Meeting of the Biophysical Society of Japan  2020.9 

    　More details

    Language：English   Presentation type：Oral presentation (general)  

    researchmap

  • Development of;a;system for;automated ionic current measurement

    Minako Hirano, Masahisa Tomita, Chikako Takahashi, Nobuyuki Kawashima, Toru Ide

    The 58th Annual Meeting of the Biophysical Society of Japan  2020.9 

    　More details

    Language：English   Presentation type：Oral presentation (general)  

    researchmap

  • 殺蚊Mpp46Abトキシン膜貫入βヘアピン内の機能構造

    下川原 澄峻, 小谷 凌也, 平野 美奈子, 井出 徹, 早川 徹

    第47回日本分子生物学会年会（福岡，2024/11/27-29） 

    　More details

▼display all

Industrial property rights

  • 人工生体膜を製造するデバイス及び製造方法

    平野 美奈子, 井出 徹

    　More details

    Applicant：学校法人光産業創成大学院大学

    Application no：特願2015-154014  Date applied：2015.8.4

    Announcement no：特開2017-029090  Date announced：2017.2.9

    Patent/Registration no：特許第6632826号  Date registered：2019.12.20 

    J-GLOBAL

    researchmap

Awards

  • Hot Article Award Analytical Sciences

    2025.5   Analytical Sciences   Direct insertion of an ion channel immobilized on a soft agarose gel bead into a lipid bilayer: an optimized method

    　More details

  • Hot Article Award Analytical Sciences

    2024.12   Analytical Sciences   Efficient single-channel current measurements of the human BK channel using a liposome-immobilized gold probe

    　More details

  • Hot Article Award Analytical Sciences

    2016.12   Analytical Sciences  

    　More details

  • 2012 Research Paper Award

    Rebeiz Foundation for Basic Research  

    　More details

  • Hiroshi and Aya Irisawa Memorial Award for Excellent Papers in The Journal of Physiological Sciences

    Simultaneous optical and electrical recording of a single ion-channel

    Toru Ide, Yuko Takeuchi, Takaaki Aoki, Toshio Yanagida

    　More details

Research Projects

  • ハイスループット単一チャネル電流記録装置の開発

    Grant number：22K06169  2022.04 - 2025.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    井出 徹

    　 More details

    Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

    researchmap

  • Single molecule measurement of ligand bindings to the acetylcholine receptor channel

    Grant number：18K06157  2018.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    井出 徹

    　 More details

    Grant amount：\4420000 （ Direct expense: \3400000 、 Indirect expense：\1020000 ）

    本研究の目的は１分子蛍光計測と単一チャネル電流記録を同時に可能とすることである。前年度までの研究成果をもとに、引き続き要素技術の改良と装置全体の整合を行った。
    i）光学系（単一チャネル電流記録法と金属増強蛍光計測の融合）：前年度までに開発した1分子観察用蛍光顕微鏡を改良、汎用性を高め、目的であるアセチルコリン受容体チャネルの計測に最適化した。
    ii）プローブ（電極）：本研究では、金属電極表面に固定したチャネル蛋白を直接人工膜に挿入する新しい電流計測法を用いている。昨年度に引き続き、試行錯誤しながら電極先端の形状等の作製条件を改良した。昨年度までは、先端が鋭利な金線、市販の金属ナノ粒子（金ナノロッド等）を蛍光増強に利用してきたが、使用可能な蛍光励起波長帯を広げるため銀電極の使用を検討した。電解研磨法の溶液・電流条件を検討することによって先端の曲率半径が100nm以下の金・銀電極を再現良く作製することが可能となった。また、実験に使用する人工膜の安定性を高めるため、電極表面の修飾法についても検討した。電極表面を疎水性分子で修飾することにより人工膜を著しく安定化させることに成功した。
    さらに副次的な成果として、上記の電流計測法を用いたイオンチャネル蛋白の高効率測定装置の開発も手がけた。金属、あるいは親水性ゲル表面に固定したチャネル蛋白を透過するイオン性電流を高効率で測定する自動計測装置の開発に成功した。これについては前年度既に報告済であるが、装置を改良し、より高効率化することに成功した。これらの測定装置を用いて、土壌細菌によるタンパク毒（Bacillus thuringiensisによるCryToxin）がイオンチャネルを形成することを発見し、それらのチャネル活性の解析も行った。

    researchmap

  • Development of a single molecule sensor with a novel method for artificial bilayer measurements.

    Grant number：15K13725  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    Ide Toru

    　 More details

    Grant amount：\3900000 （ Direct expense: \3000000 、 Indirect expense：\900000 ）

    The purpose of this study was to make a novel bio-sensor using artificial bilayers formed on the surface of a gold electrode.
    (1) Improvements of conventional method for bilayer formation: We have developed the method for bilayer formation at water/metal interface. The surface of a gold electrode was modified with hydrophilic polymers. A stable artificial bilayer was formed just by moving the tip through a lipid solution layer. Using this bilayer, we measured single channel current fluctuations of channel forming peptides such as gramicidin.(2) Protein incorporation: Ion channel proteins were immobilized on the surface of the gold electrode and these were inserted into the bilayer. The channels were incorporated into the bilayer almost simaltaneously with bilayer formation at the interface. We measured single channel currents of several types of human channel proteins using this method.

    researchmap

  • Development of High-throughput Platform for Ion Channel Analysis Using Artificial Cell Membrane Systems

    Grant number：25246017  2013.04 - 2017.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    Takeuchi Shoji, SATO Koji, IDE Toru

    　 More details

    Grant amount：\45630000 （ Direct expense: \35100000 、 Indirect expense：\10530000 ）

    In this study, we developed a high-throughput platform for analyses of ion channel functions. A cell membrane model was artificially reconstructed on a microchip, in which we optimized the efficiency and reproducibility of the membrane formation and integrated the number of the membrane on a single chip. Accordingly, we examined reconstitution techniques of ion channels into the membrane on the chip, and finally verified the developed platform using functional assays of drug-target ion channels. The results demonstrated the feasibility of our platform, especially for precise ion channel analyses with a single-molecule level. Based on the developed technologies, we continuously improve the performance of the platform, hoping for practical use in drug screening of ion channels.

    researchmap

  • Study on structure-function relationship of KcsA channel by single molecule manipulation.

    Grant number：24657110  2012.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    IDE Toru

    　 More details

    Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

    Our purpose was to develop an apparatus for simulataneous mechanical manipulation and optical recording of single ion channel protein, KcsA, by which we measure inter- and intra-molecule interactions that activate the channel. We have succeeded in (1)single molecule manipulation of KcsA immobilized at the tip of a fine glass needle (2)developing a technique for mechanically incorporating the KcsA into an artificial bilayer (3)making the measurement efficiency much higher than conventional methods by optimizing experimental conditions.

    researchmap

  • Study on channel protein gating dynamics by single molecule measurement.

    Grant number：22370059  2010.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    IDE TORU

    　 More details

    Grant amount：\19110000 （ Direct expense: \14700000 、 Indirect expense：\4410000 ）

    In order for elucidating the structure-function relationship of ion-channel proteins, we have developed an apparatus for simultaneous optical and electrical measurement of single ion-channel proteins. Using this apparatus, we successfully measured single ligand bindings to single ion-channel proteins. We developed a novel method for measuring ion-channel current in the course of apparatus development process, which is applicable to ion-channel sensor devices. We revealed a relationship between the structure and the function of potassium channel, KcsA, using macroscopic methods.

    researchmap

  • 1分子計測法によるチャネルタンパクの構造揺らぎ・機能揺らぎ相関の研究

    Grant number：21107518  2009 - 2010

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    井出 徹

    　 More details

    Grant amount：\7020000 （ Direct expense: \5400000 、 Indirect expense：\1620000 ）

    チャネルタンパクは生体膜を貫通するタンパクで、その中心にイオンを透過させる細孔を持つ。特定の刺激に応じて、細孔を開閉させることにより膜の透過性を制御している。単一チャネル電流計測技術と蛍光標識による1分子イメージング法を組み合わせれば、分子内運動による機能変化を従来法に較べてより直接的に示すことが出来るものと期待されている。この方法を用いて、チャネルタンパクの構造ゆらぎと機能の関係を見出すことが目的である。
    細菌のKチャネル(KcsA)のCys置換変異体を数種類作成し蛍光標識を行った。巨視的な蛍光計測の結果、これらのうち幾つかは、チャネルの開確率が低い(pH7)条件と高い(pH4)条件下で蛍光強度に著しい差を生じさせることが分かった。この結果からゲーティングに伴いタンパクの構造が大きく変わることを見いだした。ところが、タンパク1分子で同じ計測を行うと単一チャネル電流に見られる速い揺らぎは見えず、ゆっくりとした揺らぎが観測された。即ち、構造揺らぎと機能揺らぎが同期していない。これは、構造と機能が1対1に対応しない(機能に対応しない構造が存在する)可能性を示している。揺らぐ「状態」が機能に対応しているのかも知れない。
    上記の構造変化を確認するために、チャネルタンパクの1分子操作を行った。人工膜に組み込んだチャネルタンパクの細胞内領域を膜方向に操作することにより、活性が著しく上昇することを確認した。中性溶液中ではKcsAチャネルは殆ど活性を示さないが、ガラス微小探針等で操作することにより、酸性(pH4)条件下に匹敵する程の活性を持たせることが出来た。これは、上記蛍光計測の結果を確認するとともに新たな計測手法としても注目される。

    researchmap

  • Single-molecule study on rotary catalysis of FoF1-ATP synthase

    Grant number：18074005  2006 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Priority Areas  Grant-in-Aid for Scientific Research on Priority Areas

    NOJI Hiroyuki, TABATA Kazuhito, IINO Ryota, IDE Toru

    　 More details

    Grant amount：\225700000 （ Direct expense: \225700000 ）

    We developed a wide variety of nanobiotechnogoly, taking the advantageous features of this interdisciplinary research project. Included are single-molecule imaging method integrated with planner bilayer system, novel microdevices for single-molecule detection, novel fluorescent probe, and novel torque-determination method based on fluctuation theorem. In addition, we revealed many fundamental insights about the mehcano-chemical coupling mechanism of F_oF_1-ATPase, such as the establishment of reaction scheme of F_1, mechanical modulation of reaction equilibrium, and structural basis of cooperativity among 3 torque-generating beta subunits.

    researchmap

  • 単一チャネルタンパクの電気的・分光学的計測系の開発

    Grant number：10780412  1998

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    井出 徹

    　 More details

    Grant amount：\1200000 （ Direct expense: \1200000 ）

    researchmap

  • 骨格筋K^+チャネルの構造-機能連関の研究

    Grant number：08780623  1996

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    井出 徹

    　 More details

    Grant amount：\1000000 （ Direct expense: \1000000 ）

    我々は,イオンチャネルの3次元構造と機能の連関を解明するために,チャネルタンパク,特に骨格筋Kチャネルの構造決定と,その単分子レベルでの機能解析を行った。骨格筋細胞には横行細管膜にCa依存性Kチャネルが、筋小胞体膜には巨大コンダクタンスKチャネルが存在しているが、すでにそのファミリー遺伝子が判明していることからまずCa依存性Kチャネルのクローニングを試みた。鋳型として日本白ウサギ(オス、2週齢)の後部脚筋の白筋から得たmRNAを用い、既に明らかになっているファミリー遺伝子の相同性の高い部分を指標として,RT-PCR法により目的の遺伝子産物を増幅した。上流,及び下流の遺伝子配列は5‘RACE及び3'RACE法を用いて,現在のところCa依存性Kチャネルαサブユニットの約4割、βサブユニットの約9割の遺伝子配列を決定している。
    また,ラット大脳シナプス膜に存在する陰イオンチャネルを脂質平面膜に組み込み,チャネル活性に対する薬理学的解析を行った。その結果,このイオンチャネルは,通常の陰イオンチャネルブロッカー(DIDS,IAA,EA等)で阻害される他,細胞内のヌクレオチド(ATP,ADT,GTP)によってもブロックされることが分かった。さらに,神経伝達物質の効果を調べたところ,カテコールアミン系薬剤が強い阻害作用を示すことが分かった。また,ATP依存性Kチャネルの阻害剤として知られるグリベンクラミドが,細胞外側よりこのチャネルに対して阻害効果を示すことが分かった。

    researchmap

  • STUDIES ON MOLECULAR MECHANISM OF EXCITATION-CONTRACTION COUPLING IN SKELETAL MUSCLE

    Grant number：07458168  1995 - 1997

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    KASAI Michiki, UYEDA Atsuko, IDE Toru

    　 More details

    Grant amount：\7800000 （ Direct expense: \7800000 ）

    In order to clarify the molecular mechanism of excitation-contraction of skeletal muscle, intrinsic factors regulating excitation-contraction coupling was surveyed by using isolated sarcoplasmic reticulum (SR) vesicles. It is known that Ca^<2+> release channel (ryanodine receptor) opens by micromolar Ca^<2+> from outside the vesicles. We found that the channel became inexcitable when intravesicular Ca^<2+> concentration was lowered accompanying release of calsequestrin (CSQ). From the result, we concluded that CSQ regulates the ryanodine receptor channel. By using affinity column conjugated with CSQ,we found CSQ binds to 96-kDa triadin, 30-kDa DIDS binding protein, and 25-kDa junctin. By affinity column with 30-kDa protein, 30-kDa protein was found to bind to CSQ and junctin. As the result, we concluded that 3 proteins make ternary complex and regulate Ca^<2+> release from SR.
    Next, triads, ternary complex of transverse tubular membrane (TTM) and SR,were purified and Kinetic studies of ca^<2+> release from SR was carried out. depolarization of TTM was caused by ionic replacement, and Ca^<2+> release was assayd by using Fura-2 fluorescence. Ca^<2+> release curve could be divided into two phases. In contrast to other previous studies, in the fast phase the amount of released Ca^<2+> increased with an increase in the magnitude of depolarization but the release rate did not ; on the other hand, in the slow phase the Ca^<2+> release rate increased but the amount of Ca^<2+> did not. These dual kinetics of Ca^<2+> release were explained by the CSQ regulation model for the fast release phase.

    researchmap

  • 中枢シナプスにおける膜融合過程の研究

    Grant number：07780571  1995

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    井出 徹

    　 More details

    Grant amount：\900000 （ Direct expense: \900000 ）

    ラット大脳皮質より調製したシナプス表面膜ベシクルを、人工脂質平面膜に再構成することにより,シナプス表面膜に存在する陰イオン選択性チャネルの電気生理学的解析を行った。その結果、シナプス表面膜には、単一チャネルコンダクタンスが約50pSの比較的選択性の低い陰イオンチャネルが存在することが分かった。チャネルの開確率は比較的高く、膜電位には依存しない。開時間及び閉時間分布の解析より、このチャネルは少なくとも2つの開状態と2つの閉状態を持っていることが分かった。塩化コリン及び塩化ナトリウム溶液中での測定結果より、陰イオンと陽イオンの透過比は、P_<cl>/P_<choline>=P_<cl>/P_<na>=1.7であった。また、このチャネルは、陰イオンチャネルの阻害剤として一般に知られているSITS、EA、IAA、NPPBによって阻害されることが分かった。チャネルと一対一で結合すると仮定したとき、これらの薬剤の結合定数(K_d)は、μMから数十μM程度であった。さらに、このチャネルは細胞内側のATPによって阻害されることを発見した。バーストの解析より、ATPの効果は濃度依存的で、K_d=1.0mMであり、通常の細胞内ATPの存在下では開確率は10%以下であることが予想される。また、ATP-依存性K-チャネルの阻害剤として知られるスルホニルウレア剤(例えばグリベンクラミド)によって、このチャネルの開確率が減少することも確認した。ATP及びスルホニルウレア剤の効果は、このチャネルがATP-binding cassette(ABC)ファミリーと呼ばれる膜タンパクの族に属することを示唆している。ABCファミリーには、上記ATP-依存性K-チャネルのサブユニットと思われる膜タンパク(SUR)の他に、嚢胞性繊維症と呼ばれる疾患に関与するcystic fibrosis transmembrane conductance regulator(CFTR)も属しており、これらとの関連からも興味深い。

    researchmap

  • 筋小胞体イオンチャネルの構造機能連関の解析

    Grant number：06858071  1994

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    井出 徹

    　 More details

    Grant amount：\900000 （ Direct expense: \900000 ）

    1.我々は、脂質平面膜法を検定系として、ウサギ骨格筋筋小胞体チャネルタンパクの生化学的精製に取り組んできたが、その結果、分子量138k及び100kのタンパクのみを含む分画にClチャネル活性があることを見い出した。これらの内、チャネル本体を形成するのは100kタンパクと思われ、138kタンパクはその制御因子である可能性が高いと予想された。138kタンパクの部分アミノ酸配列を数カ所について決定し、相同性の検索を行ったところ、筋小胞体膜タンパクでありカルシウム結合機能を持つと報告されている160kタンパクに、全く同一の配列があることが分かった。当初、共通の遺伝子から異なるスプライシング制御を受けて作られたものと思われたが、さらに、生化学的解析を行ったところこれら2つのタンパクは全く同一のものと判明した。分子量の違いは、前に160kタンパクの報告を行ったグループの算定ミスである可能性が高い。次に、138kタンパクの特異的抗体を作成して、筋小胞体ベシクルのイオン透過性に及ぼす効果を調べたところ(光散乱法による)、KCl透過の比較的遅い成分を阻害することが分かった。この効果がClチャネルまたはKチャネルのブロックによるものか、あるいは他の経路によるのか今のところ定かではない。
    2.脂質平面膜法用いて、ウサギ骨格筋横行細管(T-tubule)に存在するClチャネルの電気生理学的解析を行った。その結果、T-tubule膜には単一チャネルコンダクタンス40pSのバックグラウンドタイプ(静止電位付近でも高い開確率を示す)Clチャネルが存在することが分かった。このチャネルは電位依存性陰イオンチャネルのブロッカーとして一般に知られる9-AC,DIDS,SITS,EAで阻害されることが分かった。さらに、気管上皮細胞のClチャネル阻害剤として開発されたIAA-94によってもμM程度の濃度でブロックされることが分かった。

    researchmap

  • 脂質平面膜法を用いた骨格筋筋小胞体イオンチャンネルの精製及び構造解析

    Grant number：05858092  1993

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    井出 徹

    　 More details

    Grant amount：\1100000 （ Direct expense: \1100000 ）

    我々は、脂質平面膜法を検定系として、筋小胞体チャネルの精製を試みてきたが、ConA-Sepharose及び陰イオン交換クロマトグラフィーによる分離した分子量が138kDaと100kDaの糖タンパクを含む分画にのみCI-チャネル活性が認められた。阻害剤の結合実験の結果などにより、このうち、100kDaタンパクがCI-チャネルと推測される。一方、二つの糖タンパクが様々なクロマトグラフィーで必ず同じ分画に存在することから、138k糖タンパクはチャネルを制御するタンパクである可能性が考えられる。電気泳動的に目的のタンパクを抽出した138kDa糖タンパクを抗原として、マウスを用いてポリクローナル抗体を作製したところ138kDa糖タンパクの他に160kDa、53kDa糖タンパクとも反応を示した。また、138kDa糖タンパクの部分アミノ酸配列の決定に成功し、相同性の検索を行ったところ、160kDa、53kDa糖タンパクの共通アミノ酸配列を持つことが分かった。以上より、これらのタンパクが共通の遺伝子からスプライシング制御を受けてつくられたタンパクであることが示唆される。また、138kDa糖タンパクのアフィニティカラムを作製してその結合タンパクを探索したところ、0.1M,0.3M KC1で溶出される分画に100kDaのタンパクが存在した。これをベシクルに再構成して脂質平面膜に組み込んだところ、0.3M KC1溶出分画でカチオンチャネル活性が観測されたが、C1-チャネル活性は観測されなかった。このチャネル活性はNeomycinにより阻害されたのでSRのmaxi-K^+チャネルである可能性がある。
    また、骨格筋のmRNAを注入する事によって、アフリカツメガエルの卵母細胞表面膜に、筋小胞体チャネルを発現されることに成功した。

    researchmap

  • ISOLATION OF ION CHANNEL MOLECULES BY USING LIPID BILAYER TECHNIQUE AS AN ASSAYING SYSTEM AND STUDY ON THEIR STRUCTURE AND FUNCTION RELATIONSHIP

    Grant number：04454620  1992 - 1994

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (B)  Grant-in-Aid for General Scientific Research (B)

    KASAI Michiki, IDE Toru, TAGUCHI Takahisa

    　 More details

    Grant amount：\6300000 （ Direct expense: \6300000 ）

    Purification of ion channels in sacrcoplasmic (SR) of rabbit skeletal muscle has been carried out by assaying the channel activity in the lipid bilayr system. As a result, the fraction which contains 138 and 100 kDa protein showed CI^- channel activity. After purification of 138 kDa protein, partial amino acid sequences were determined and their homegogy was referred. The 138 kDa protein was found to be the same one as that reported as 160 kDa protein which has Ca^<2+>-binding activity. The difference was due to the nuderestimation of the previous workers. The 138 kDa protein interacts with 100 kDa protein in SR.When 100 kDa protein was incorporated into lipid bilayr, it showed channel activity. It was suggested that 138 and 100 kDa proteins worked as ion channle by making complex.Antibody against 138 kDa protein inhibited slow KCI permaetion through SR vesicles.
    CI^- channel activity in transverse tubule (T-tubule) in rabbit skeletal muscle was investigated by lipid bilayr system. A back-ground type CI^- channel (open at resting potential) having 40 pS was found. This channel was blocked by 9-AC,DIDS,SITS,and EA which were known as blockers againg voltage dependent anion channels. Further, the channel was blocked by IAA-94 which was developed as a blocker for CI^- channel in apical membrane from trachea. Similarity between these channels was suggested. Antibody which blocked the channel activity recognized 90 kDa protein in muscle.

    researchmap

  • 脂質平面膜法を用いた骨格筋筋小胞体K^+-チャンネル及びCl^--チャンネルの精製

    Grant number：03858074  1991

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    井出 徹

    　 More details

    Grant amount：\900000 （ Direct expense: \900000 ）

    researchmap

▼display all

 

Class subject in charge

  • Internship （2025academic year） Summer concentration  - その他

  • Internship （2025academic year） Summer concentration  - その他

  • Internship （2025academic year） Summer concentration  - その他

  • BioNanoTechnology （2025academic year） Third semester  - 月1～2

  • General Biotechnology and Drug Discovery Technology （2025academic year） Prophase  - 木1～2

  • Advanced Internship for Interdisciplinary Medical Sciences and Engineering （2025academic year） Year-round  - その他

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2025academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2025academic year） Year-round  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2025academic year） Year-round  - その他

  • Molecular physiology （2025academic year） Prophase  - 木7～8

  • Technical Writing and Presentation （2025academic year） Third semester  - 月5～6

  • Technical Writing and Presentation （2025academic year） Late  - その他

  • Biochemistry 2 （2025academic year） Second semester  - 月3～4,木1～2

  • Biochemistry 2 （2025academic year） Second semester  - 月3～4,木1～2

  • Basic Biology （2025academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2025academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2025academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2025academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology 1 （2025academic year） Third semester  - 金1～2

  • Basic Biology 1 （2025academic year） Third semester  - 金1～2

  • Basic Biology 1 （2025academic year） Third semester  - 金1～2

  • Basic Biology 2 （2025academic year） Fourth semester  - 金1～2

  • Basic Biology 2 （2025academic year） Fourth semester  - 金1～2

  • Basic Biology 2 （2025academic year） Fourth semester  - 金1～2

  • Biophysics （2025academic year） Third semester  - 月1～2

  • Single molecule physiology （2025academic year） Late  - その他

  • BioNanoTechnology （2024academic year） Third semester  - 月1～2

  • General Biotechnology and Drug Discovery Technology （2024academic year） Prophase  - 木1～2

  • Advanced Internship for Interdisciplinary Medical Sciences and Engineering （2024academic year） Year-round  - その他

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2024academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2024academic year） Year-round  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2024academic year） Year-round  - その他

  • Introduction to Chemistry and Bioengineering （2024academic year） 1st semester  - その他

  • Technical Writing and Presentation （2024academic year） Third semester  - 月5～6

  • Technical Writing and Presentation （2024academic year） Late  - その他

  • Biochemistry 2 （2024academic year） Second semester  - 月3～4,木1～2

  • Biochemistry 2 （2024academic year） Second semester  - 月3～4,木1～2

  • Basic Biology （2024academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2024academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2024academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2024academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2024academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2024academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology 1 （2024academic year） Third semester  - 金1～2

  • Basic Biology 1 （2024academic year） Third semester  - 金1～2

  • Basic Biology 1 （2024academic year） Third semester  - 金1～2

  • Basic Biology 2 （2024academic year） Fourth semester  - 金1～2

  • Basic Biology 2 （2024academic year） Fourth semester  - 金1～2

  • Basic Biology 2 （2024academic year） Fourth semester  - 金1～2

  • Biophysics （2024academic year） Third semester  - 月1～2

  • Single molecule physiology （2024academic year） Late  - その他

  • BioNanoTechnology （2023academic year） Third semester  - 月1～2

  • General Biotechnology and Drug Discovery Technology （2023academic year） Prophase  - 木1～2

  • Advanced Internship for Interdisciplinary Medical Sciences and Engineering （2023academic year） Year-round  - その他

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2023academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2023academic year） Year-round  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2023academic year） Year-round  - その他

  • Molecular physiology （2023academic year） Prophase  - 木7～8

  • Introduction to Chemistry and Bioengineering （2023academic year） 1st semester  - その他

  • Technical Writing and Presentation （2023academic year） Third semester  - 月5～6

  • Technical Writing and Presentation （2023academic year） Late  - その他

  • Biochemistry 2 （2023academic year） Second semester  - 月3～4,木1～2

  • Biochemistry 2 （2023academic year） Second semester  - 月3～4,木1～2

  • Basic Biology （2023academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2023academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2023academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2023academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2023academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2023academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology 1 （2023academic year） Third semester  - 金1～2

  • Basic Biology 1 （2023academic year） Third semester  - 金1～2

  • Basic Biology 1 （2023academic year） Third semester  - 金1～2

  • Basic Biology 2 （2023academic year） Fourth semester  - 金1～2

  • Basic Biology 2 （2023academic year） Fourth semester  - 金1～2

  • Basic Biology 2 （2023academic year） Fourth semester  - 金1～2

  • Biophysics （2023academic year） Third semester  - 月1～2

  • Single molecule physiology （2023academic year） Late  - その他

  • General Biotechnology and Drug Discovery Technology （2022academic year） Prophase  - その他

  • General Biotechnology and Drug Discovery Technology （2022academic year） Prophase  - 木1～2

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2022academic year） Late  - その他

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2022academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2022academic year） Year-round  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2022academic year） Year-round  - その他

  • Molecular physiology （2022academic year） Prophase  - 不開講

  • Basic of Applied Chemistry and Biotechnology （2022academic year） 1st semester  - 金1～2

  • Introduction to Chemistry and Bioengineering （2022academic year） 1st semester  - その他

  • Technical Writing and Presentation （2022academic year） 3rd and 4th semester  - 月5～6

  • Technical Writing and Presentation （2022academic year） 3rd and 4th semester  - 月5～6

  • Technical Writing and Presentation （2022academic year） Late  - その他

  • Technical Writing and Presentation （2022academic year） Late  - その他

  • Biochemistry and Exercises 2 （2022academic year） Second semester  - 月3～4,木3～4

  • Biochemistry 2 （2022academic year） Second semester  - 月3～4,木1～2

  • Biochemistry 2 （2022academic year） Second semester  - 月3～4,木1～2

  • Basic Biology （2022academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2022academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2022academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2022academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2022academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology （2022academic year） 3rd and 4th semester  - 金1～2

  • Basic Biology 1 （2022academic year） Third semester  - 金1～2

  • Basic Biology 1 （2022academic year） Third semester  - 金1～2

  • Basic Biology 1 （2022academic year） Third semester  - 金1～2

  • Basic Biology 2 （2022academic year） Fourth semester  - 金1～2

  • Basic Biology 2 （2022academic year） Fourth semester  - 金1～2

  • Basic Biology 2 （2022academic year） Fourth semester  - 金1～2

  • Biophysics （2022academic year） Second semester  - 月1～2,木3～4

  • Single molecule physiology （2022academic year） Late  - その他

  • General Biotechnology and Drug Discovery Technology （2021academic year） Prophase  - その他

  • General Biotechnology and Drug Discovery Technology （2021academic year） Prophase  - 木1～2

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2021academic year） Late  - その他

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2021academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2021academic year） Year-round  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2021academic year） Year-round  - その他

  • Molecular physiology （2021academic year） Prophase  - 木7～8

  • Introduction to Chemistry and Bioengineering （2021academic year） special  - その他

  • Introduction to Chemistry and Bioengineering （2021academic year） special  - その他

  • Technical Writing and Presentation （2021academic year） 3rd and 4th semester  - 月5,月6,月7,月8

  • Technical Writing and Presentation （2021academic year） 3rd and 4th semester  - 月5～6

  • Technical Writing and Presentation （2021academic year） Late  - その他

  • Technical Writing and Presentation （2021academic year） Late  - その他

  • Biochemistry and Exercises 2 （2021academic year） Third semester  - 月5,月6,木1,木2

  • Biochemistry 2 （2021academic year） Third semester  - 月5,月6,木1,木2

  • Basic Biology （2021academic year） 3rd and 4th semester  - 金1,金2

  • Basic Biology （2021academic year） 3rd and 4th semester  - 金1,金2

  • Basic Biology （2021academic year） 3rd and 4th semester  - 金1,金2

  • Basic Biology （2021academic year） 3rd and 4th semester  - 金1,金2

  • Basic Biology （2021academic year） 3rd and 4th semester  - 金1,金2

  • Basic Biology （2021academic year） 3rd and 4th semester  - 金1,金2

  • Basic Biology 1 （2021academic year） Third semester  - 金1,金2

  • Basic Biology 1 （2021academic year） Third semester  - 金1,金2

  • Basic Biology 1 （2021academic year） Third semester  - 金1,金2

  • Basic Biology 2 （2021academic year） Fourth semester  - 金1,金2

  • Basic Biology 2 （2021academic year） Fourth semester  - 金1,金2

  • Basic Biology 2 （2021academic year） Fourth semester  - 金1,金2

  • Biophysics （2021academic year） Second semester  - 月3,月4,木1,木2

  • Single molecule physiology （2021academic year） Late  - その他

  • General Biotechnology and Drug Discovery Technology （2020academic year） Prophase  - 木1,木2

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2020academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2020academic year） Year-round  - その他

  • Introduction to Chemistry and Bioengineering （2020academic year） Summer concentration  - その他

  • Introduction to Chemistry and Bioengineering （2020academic year） Summer concentration  - その他

  • Technical Writing and Presentation （2020academic year） 3rd and 4th semester  - 月5,月6

  • Technical Writing and Presentation （2020academic year） Late  - その他

  • Biochemistry and Exercises 2 （2020academic year） Third semester  - 月5,月6,木1,木2

  • Biochemistry 2 （2020academic year） Third semester  - 月5,月6,木1,木2

  • Basic Biology （2020academic year） 3rd and 4th semester  - 金1,金2

  • Basic Biology （2020academic year） 3rd and 4th semester  - 金1,金2

  • Basic Biology （2020academic year） 3rd and 4th semester  - 金1,金2

  • Basic Biology 1 （2020academic year） Third semester  - 金1,金2

  • Basic Biology 1 （2020academic year） Third semester  - 金1,金2

  • Basic Biology 1 （2020academic year） Third semester  - 金1,金2

  • Basic Biology 2 （2020academic year） Fourth semester  - 金1,金2

  • Basic Biology 2 （2020academic year） Fourth semester  - 金1,金2

  • Basic Biology 2 （2020academic year） Fourth semester  - 金1,金2

  • Biophysics （2020academic year） Second semester  - 月3,月4,木1,木2

  • Single molecule physiology （2020academic year） Late  - その他

▼display all