2022/04/14 更新

写真a

ナカムラ ヨシマサ
中村 宜督
NAKAMURA Yoshimasa
所属
環境生命科学学域 教授
職名
教授
外部リンク

学位

  • 博士(農学) ( 京都大学 )

研究キーワード

  • 食品化学

研究分野

  • ライフサイエンス / 食品科学

学歴

  • 京都大学    

    1995年4月 - 1998年3月

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  • 京都大学    

    1993年4月 - 1995年3月

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  • 京都大学    

    1989年4月 - 1993年3月

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経歴

  • 岡山大学大学院環境生命科学研究科   教授

    2014年12月 - 現在

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  • 岡山大学環境生命科学研究科   准教授

    2012年4月 - 2014年11月

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  • 岡山大学自然科学研究科   准教授

    2007年4月 - 2012年3月

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  • イリノイ大学シカゴ校   特別訪問研究員

    2006年10月 - 2008年3月

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  • 岡山大学大学院自然科学研究科   助教授

    2005年4月 - 2007年3月

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  • 名古屋大学大学院生命農学研究科   助手

    2000年8月 - 2005年4月

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  • 京都大学総合人間学部   非常勤講師

    1998年10月 - 1999年3月

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  • 日本学術振興会特別研究員(PD)

    1998年4月 - 2000年7月

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▼全件表示

 

論文

  • A Major Intestinal Catabolite of Quercetin Glycosides, 3-Hydroxyphenylacetic Acid, Protects the Hepatocytes from the Acetaldehyde-Induced Cytotoxicity through the Enhancement of the Total Aldehyde Dehydrogenase Activity 査読

    Yujia Liu, Takumi Myojin, Kexin Li, Ayuki Kurita, Masayuki Seto, Ayano Motoyama, Xiaoyang Liu, Ayano SATOH, Shintaro Munemasa, Yoshiyuki Murata, Toshiyuki Nakamura, Yoshimasa Nakamura

    International Journal of Molecular Sciences   2022年2月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/ijms23031762

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  • White rice ethanol extract is qualitatively, but not quantitatively, equivalent to that of brown rice as an antioxidant source. 査読 国際誌

    Hongyan Wu, Toshiyuki Nakamura, Yingnan Guo, Miho Hirooka, Gongliang Zhang, Shintaro Munemasa, Yoshiyuki Murata, Akiko Fujita, Yoshimasa Nakamura

    Bioscience, biotechnology, and biochemistry   85 ( 10 )   2161 - 2168   2021年9月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purpose of this study is to compare the potentials to exhibit biologically active antioxidant actions between white rice (WR) and brown rice (BR) in in vitro assays and a cellular model. The Trolox equivalent (TE) per 1 mg ethanol extract of WR for the 1,1-diphenyl-2-picrylhydrazyl assay was slightly higher than that of BR, whereas the TE per 1 g whole WR was much lower than that for BR. This tendency was very comparable to those for the oxygen radical absorbance capacity and total polyphenol content. Both of the ethanol extracts also similarly suppressed the hydrogen peroxide-induced cytotoxicity and enhanced the gene expression of drug-metabolizing enzymes. Based on the α-tocopherol quantity, its contribution to the cytoprotective effect of the rice extracts is very limited. Taken together, the ethanol extract of WR might be a qualitatively, but not quantitatively, equivalent source of antioxidative phytochemicals to that of BR.

    DOI: 10.1093/bbb/zbab133

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  • Inhibition of Drug Resistance Mechanisms Improves the Benzyl Isothiocyanate–Induced Anti-Proliferation in Human Colorectal Cancer Cells 招待 査読

    Xiaoyang Liu, Qifu Yang, Yoshimasa Nakamura

    Current Pharmacology Reports   2020年10月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media {LLC}  

    DOI: 10.1007/s40495-020-00227-4

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  • Characterization of benzyl isothiocyanate extracted from mashed green papaya by distillation. 査読

    Nakamura T, Murata Y, Nakamura Y

    Food chemistry   299   125118   2019年11月

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    担当区分:最終著者, 責任著者  

    DOI: 10.1016/j.foodchem.2019.125118

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  • Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12. 査読 国際誌

    Naomi Abe-Kanoh, Narumi Kunisue, Takumi Myojin, Ayako Chino, Shintaro Munemasa, Yoshiyuki Murata, Ayano Satoh, Hisao Moriya, Yoshimasa Nakamura

    Scientific reports   9 ( 1 )   8866 - 8866   2019年6月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Benzyl isothiocyanate (BITC) is a naturally-occurring isothiocyanate derived from cruciferous vegetables. BITC has been reported to inhibit the proliferation of various cancer cells, which is believed to be important for the inhibition of tumorigenesis. However, the detailed mechanisms of action remain unclear. In this study, we employed a budding yeast Saccharomyces cerevisiae as a model organism for screening. Twelve genes including MTW1 were identified as the overexpression suppressors for the antiproliferative effect of BITC using the genome-wide multi-copy plasmid collection for S. cerevisiae. Overexpression of the kinetochore protein Mtw1 counteracts the antiproliferative effect of BITC in yeast. The inhibitory effect of BITC on the proliferation of human colon cancer HCT-116 cells was consistently suppressed by the overexpression of Mis12, a human orthologue of Mtw1, and enhanced by the knockdown of Mis12. We also found that BITC increased the phosphorylated and ubiquitinated Mis12 level with consequent reduction of Mis12, suggesting that BITC degrades Mis12 through an ubiquitin-proteasome system. Furthermore, cell cycle analysis showed that the change in the Mis12 level affected the cell cycle distribution and the sensitivity to the BITC-induced apoptosis. These results provide evidence that BITC suppresses cell proliferation through the post-transcriptional regulation of the kinetochore protein Mis12.

    DOI: 10.1038/s41598-019-45248-2

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  • Physiological relevance of covalent protein modification by dietary isothiocyanates 査読

    Toshiyuki Nakamura, Naomi AbeKanoh, Yoshimasa Nakamura

    Journal of Clinical Biochemistry and Nutrition   62 ( 1 )   11 - 19   2018年1月

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    担当区分:最終著者, 責任著者   記述言語:英語   出版者・発行元:The Society for Free Radical Research Japan  

    Isothiocyanates (ITCs), naturally occurring in abundance in crucif erous vegetables, are the most wellstudied organosulfur com pounds having an electrophilic reactivity. ITCs have been accepted as major ingredients of these vegetables that afford their health promoting potentials. ITCs are able to modulate protein functions related to drugmetabolizing enzymes, transporters, kinases and phosphatases, etc. One of the most important questions about the molecular basis for the health promoting effects of ITCs is how they modulate cellular target proteins. Although the molec ular targets of ITCs remains to be validated, dietary modulation of the target proteins via covalent modification by ITCs should be one of the promising strategies for the protection of cells against oxidative and inflammatory damage. This review discusses the plausible target proteins of dietary ITCs with an emphasis on pos sible involvement of protein modification in their health promot ing effects. The fundamental knowledge of ITCs is also included with consideration of the chemistry, intracellular behavior, and metabolism.

    DOI: 10.3164/jcbn.17-91

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  • Flavonoid metabolism: the interaction of metabolites and gut microbiota 査読

    Kaeko Murota, Yoshimasa Nakamura, Mariko Uehara

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   82 ( 4 )   600 - 610   2018年

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    担当区分:筆頭著者   記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    Several dietary flavonoids exhibit anti-oxidative, anti-inflammatory, and anti-osteoporotic activities relevant to prevention of chronic diseases, including lifestyle-related diseases. Dietary flavonoids (glycoside forms) are enzymatically hydrolyzed and absorbed in the intestine, and are conjugated to their glucuronide/sulfate forms by phase II enzymes in epithelial cells and the liver. The intestinal microbiota plays an important role in the metabolism of flavonoids found in foods. Some specific products of bacterial transformation, such as ring-fission products and reduced metabolites, exhibit enhanced properties. Studies on the metabolism of flavonoids by the intestinal microbiota are crucial for understanding the role of these compounds and their impact on our health. This review focused on the metabolic pathways, bioavailability, and physiological role of flavonoids, especially metabolites of quercetin and isoflavone produced by the intestinal microbiota.

    DOI: 10.1080/09168451.2018.1444467

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  • Benzyl isothiocyanate ameliorates acetaldehyde-induced cytotoxicity by enhancing aldehyde dehydrogenase activity in murine hepatoma Hepa1c1c7 cells 査読

    Yujia Liu, Momoko Yamanaka, Naomi Abe-Kanoh, Xiaoyang Liu, Beiwei Zhu, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura

    FOOD AND CHEMICAL TOXICOLOGY   108   305 - 313   2017年10月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    In the present study, we assessed benzyl isothiocyanate (BITC), an organosulfur compound from cruciferous vegetables, as a potential inducer of aldehyde dehydrogenase (ALDH) activity using murine hepatoma Hepa1c1c7 cells. BITC was shown to enhance not only the total ALDH activity, but also the ALDH activity of the cytosolic/microsomal and mitochondrial fraction. BITC also significantly increased the gene and protein expression of ALDH1A1, ALDH2 and ALDH3A1 in a concentration-dependent manner. Simultaneously, the gene expression of phase 2 drug-metabolizing enzymes, such as NAD(P)H: quinone oxidoreductase 1 and heme oxygenase-1, was increased by the BITC treatment. Western blot experiments revealed that BITC not only up-regulated the Nrf2 protein expression, but also stimulated the nuclear translocation of Nrf2. Furthermore, silencing Nrf2 reduced the basal and BITC-enhanced levels of the total activity and gene expression of ALDHs. The pretreatment of BITC completely mitigated the acetaldehyde-induced cytotoxicity, which was impaired by silencing Nrf2. The present study demonstrated that BITC has been identified as a potential inducer of the total ALDH activity to prevent the acetaldehyde-induced cytotoxicity. (C) 2017 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.fct.2017.08.016

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  • 3,4-Dihydroxyphenylacetic acid is a predominant biologically-active catabolite of quercetin glycosides 査読

    Yue Tang, Sayaka Nakashima, Shunya Saiki, Yui Myoi, Naomi Abe, Shoko Kuwazuru, Beiwei Zhu, Hitoshi Ashida, Yoshiyuki Murata, Yoshimasa Nakamura

    FOOD RESEARCH INTERNATIONAL   89   716 - 723   2016年11月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Since dietary flavonoid glycosides, including quercetin 4'-glucoside from onion, are poorly absorbed from the gastrointestinal tract, they are converted into smaller phenolic acids, which can be absorbed into the circulation. The purpose of this study was to compare the effects of the major phenolic acid catabolites of quercetin 4'-glucoside, including 3,4-dihydroxyphenylacetic acid (DOPAC), 3-hydroxyphenylacetic acid, 3,4-dihydroxybenzoic acid (protocatechuic acid) and hippuric acid, on the antioxidant activity and phase II cytoprotective enzyme induction in vitro. Both DOPAC and protocatechuic acid, having a catechol moiety, exhibited both DPPH radical scavenging and superoxide dismutase-like activities, whereas 3-hydroxyphenyl acetic acid and hippuric acid did not. DOPAC also more potently enhanced the gene expression of several phase II drug-metabolizing enzymes than the other phenolic acid catabolites. DOPAC significantly inhibited the hydrogen peroxide-induced cytotoxicity in hepatocytes with the enhancement of the total glutathione S-transferase activity. In conclusion, DOPAC may play a key role in the antioxidative potential of the colonic lumen after the ingestion of the quercetin glycoside-rich onion. (C) 2016 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.foodres.2016.09.034

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  • Nuclear factor-kappaB sensitizes to benzyl isothiocyanate-induced antiproliferation in p53-deficient colorectal cancer cells 査読

    N. Abe, D-X Hou, S. Munemasa, Y. Murata, Y. Nakamura

    CELL DEATH & DISEASE   5   2014年11月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Benzyl isothiocyanate (BITC), a dietary isothiocyanate derived from cruciferous vegetables, inhibits the proliferation of colorectal cancer cells, most of which overexpress beta-catenin as a result of mutations in the genes for adenomatous polyposis coli or mutations in beta-catenin itself. Because nuclear factor-kappa B (NF-kappa B) is a plausible target of BITC signaling in inflammatory cell models, we hypothesized that it is also involved in BITC-inhibited proliferation of colorectal cancer cells. siRNA-mediated knockdown of the NF-kappa B p65 subunit significantly decreased the BITC sensitivity of human colorectal cancer HT-29 cells with mutated p53 tumor suppressor protein. Treating HT-29 cells with BITC induced the phosphorylation of I kappa B kinase, I kappa B-alpha and p65, the degradation of I kappa B-a, the translocation of p65 to the nucleus and the upregulation of NF-kappa B transcriptional activity. BITC also decreased alpha-catenin binding to a positive cis element of the cyclin D1 promoter and thus inhibited beta-catenin-dependent cyclin D1 transcription, possibly through a direct interaction between p65 and alpha-catenin. siRNA-mediated knockdown of p65 confirmed that p65 negatively affects cyclin D1 expression. On the other hand, when human colorectal cancer HCT-116 cells with wild-type p53 were treated with BITC, translocation of p65 to the nucleus was inhibited rather than enhanced. p53 knockout increased the BITC sensitivity of HCT-116 cells in a p65-dependent manner, suggesting that p53 negatively regulates p65-dependent effects. Together, these results identify BITC as a novel type of antiproliferative agent that regulates the NF-kappa B pathway in p53-deficient colorectal cancer cells.

    DOI: 10.1038/cddis.2014.495

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  • Influence of fish skin gelatin-sodium alginate complex stabilized emulsion on benzyl isothiocyanate stability and digestibility in vitro and in vivo. 査読 国際誌

    Xiangyu Zhang, Zhiyu Liu, Ailin Wang, Simin Zhang, Yoshimasa Nakamura, Songyi Lin, Yue Tang

    Journal of the science of food and agriculture   2022年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: An emulsion delivery system for benzyl isothiocyanate (BITC) was prepared using fish skin gelatin (FSG) and sodium alginate (Alg). The effects of the FSG-Alg complex on the emulsion stability and BITC release pattern from the emulsion were investigated in vitro and in vivo. RESULTS: The storage stability and embedding rate of the 10 g/kg FSG and 2.5 g/kg Alg (FSG-Alg) emulsion were the highest among all samples. The FSG-Alg complex provided BITC a better protection during in vitro digestion. The microstructure of the FSG-Alg emulsions was more stable during in vitro digestion, and the bioaccessibility and retention rate of BITC were much higher compared to those of the FSG emulsion. The results of the ex-vivo everted gut sac of rat intestine study showed that the FSG-Alg emulsion significantly increased the BITC absorption rate in the duodenum. CONCLUSION: The FSG-Alg emulsion delivery system is a highly stable system for the delivery of BITC that improves the bioaccessibility of BITC and promotes its absorption in the duodenum. This article is protected by copyright. All rights reserved.

    DOI: 10.1002/jsfa.11915

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  • Characterization of a synthetic zinc-chelating peptide from sea cucumber (Stichopus japonicus) and its gastrointestinal digestion and absorption in vitro. 査読 国際誌

    Zixu Wang, Jiatong Sun, Xiaoyu Ma, Xiaoyang Liu, Fawen Yin, Deyang Li, Yoshimasa Nakamura, Chenxu Yu, Dayong Zhou

    Journal of the science of food and agriculture   2022年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Zinc absorption in intestinal system could be strongly affected by the gastrointestinal digestion and absorption of zinc-chelating peptides serving as zinc carriers. In this study, a novel zinc-chelating sea cucumber synthetic peptide (SCSP) was synthesized to estimate its gastrointestinal digestion and promotive effect of zinc absorption in vitro. RESULTS: Analysis of isothermal titration calorimetry suggested that the binding of SCSP and zinc (N ≈ 1) was exothermic, with relatively weak binding affinity (K = 1.0х10-3 mol L-1 ). The formation of SCSP-Zn complexes brought morphological changes to the peptides confirmed by scanning electron microscopy (SEM), which also indicated 6.88% of the existence of zinc element. In addition, the SCSP-Zn complexes remained stable under simulated human gastrointestinal digestion. In vitro study, the SCSP-Zn complex could successfully transport through the intestinal membrane in the model of everted rat gut sacs (nearly 7.5 μM cm-2 ) as well as Caco-2 cells where the zinc transport reached 0.0014 mg mL-1 carried by SCSP. Fluorescence staining experiments revealed free zinc accumulation inside the tissues and cells treated with the SCSP-Zn. CONCLUSIONS: The chelation SCSP-Zn had the promotion ability of zinc absorption in vitro and ex vivo experiments, which suggested a theoretical basis for the design and production of effective zinc chelating peptides as zinc carriers to improve zinc bioavailability. This article is protected by copyright. All rights reserved.

    DOI: 10.1002/jsfa.11811

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  • Gastrointestinal digestion and absorption characterization in vitro of zinc-chelating hydrolysate from scallop adductor (Patinopecten yessoensis). 査読 国際誌

    Jiatong Sun, Xiaoyang Liu, Zixu Wang, Fawen Yin, Huilin Liu, Yoshimasa Nakamura, Chenxu Yu, Dayong Zhou

    Journal of the science of food and agriculture   2021年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUD: Zinc is an essential catalytic element in human health system but its absorption in intestinal system could be strongly affected by the gastrointestinal digestion. In this study, the food-derived potential zinc carrier, scallop adductor hydrolysates (SAHs), was produced and characterized. RESULTS: During the temporary storage in 4°C, the SAH decreased in zinc-chelating capacity in aqueous phase whereas the SAH-Zn complex exhibited high stability. Moreover, the secondary structure of SAH had no significant alteration. Zinc morphologically altered the surface structures of SAH, which was involving in carboxyl group of SAH. Results of in vitro gastrointestinal (GI) digestion suggested that the SAH-Zn maintained good stability in GI system and only proportion of high molecular weight cleaved. In addition, SAH could successfully carry and transport zinc while the fluorescence staining revealed free zinc accumulation inside the tissue. Finally, 3 representative absorbed peptides (around 600 Da) were identified and synthesized. Three synthetic peptides exhibit higher zinc-chelating capacity than SAH and could also successfully transported through the intestine. CONCLUSION: This study provided a theoretical basis for the investigation of digestion and absorption of marine animal-derived peptides as zinc carriers. This article is protected by copyright. All rights reserved.

    DOI: 10.1002/jsfa.11673

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  • Neither glutamate nor alanine but arginine sensitizes BY-2 cells to arsenate. 査読 国際誌

    Nur-E-Nazmun Nahar, Md Yeasin Prodhan, Yoshiharu Mimata, Anna Yonezawa, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

    Bioscience, biotechnology, and biochemistry   2021年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Arsenic is toxic for plants. Our previous results showed that the application of proline enhanced the sensitivity of tobacco BY-2 cells to arsenate. In order to clarify the enhancement mechanism, we investigated the effects of other amino acids on the arsenate-stressed BY-2 cells. Glutamate at up to 10 mM did not affect the cell growth in the absence or presence of arsenate. Arginine at up to 10 mM did not affect the growth in the absence of arsenate but arginine at 10 mM enhanced the inhibition of the cell growth by arsenate. Alanine at up to 10 mM did not affect the cell growth under non-stressed condition but alanine at 10 mM significantly improved the cell growth under arsenate stress. These results suggest that alanine mitigates arsenate stress in BY-2 cells and that arginine like proline enhances the sensitivity of BY-2 cells to arsenate.

    DOI: 10.1093/bbb/zbab191

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  • Cadmium uptake via apoplastic bypass flow in Oryza sativa. 査読

    Izumi C Mori, Carlos Raul Arias-Barreiro, Lia Ooi, Nam-Hee Lee, Muhammad Abdus Sobahan, Yoshimasa Nakamura, Yoshihiko Hirai, Yoshiyuki Murata

    Journal of plant research   134 ( 5 )   1139 - 1148   2021年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    It is known that rice roots take up cadmium (Cd) via the symplastic route mediated by membrane-bound mineral transporters. Here we provide evidence that apoplastic bypass flow is another Cd uptake route in rice. High concentrations of Cd rendered apoplastic bypass flow rate increased in rice seedlings. These concentrations of Cd compromised membrane integrity in the root meristem and transition zone. Polyethleneglycol and proline inhibited the Cd-induced apoplastic bypass flow and Cd transfer to the shoots. Loss-of-function mutant of the Cd uptake transporter, nramp5, showed Cd transport to the shoot comparable to the wild type. At a low Cd concentration, increased apoplastic bypass flow rate by NaCl stress resulted in an elevation of Cd transport to shoots both in the wildtype and nramp5. These observations indicate that apoplastic bypass flow in roots carries Cd transport leading to xylem loading of Cd in addition to the symplastic pathway mediated by mineral transporters under stressed conditions.

    DOI: 10.1007/s10265-021-01319-y

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  • Modulation of frequency and height of cytosolic calcium spikes by plasma membrane anion channels in guard cells. 査読 国際誌

    Md Tahjib-Ul-Arif, Shintaro Munemasa, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoshiyuki Murata

    Bioscience, biotechnology, and biochemistry   85 ( 9 )   2003 - 2010   2021年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cytosolic calcium ([Ca2+]cyt) elevation activates plasma membrane anion channels in guard cells, which is required for stomatal closure. However, involvement of the anion channels in the [Ca2+]cyt elevation remains unclear. We investigated the involvement using Arabidopsis thaliana anion channel mutants, slac1-4 slah3-3 and slac1-4 almt12-1. Extracellular calcium induced stomatal closure in the wild-type plants but not in the anion channel mutant plants whereas extracellular calcium induced [Ca2+]cyt elevation both in the wild-type guard cells and in the mutant guard cells. The peak height and the number of the [Ca2+]cyt spike were lower and larger in the slac1-4 slah3-3 than in the wild type and the height and the number in the slac1-4 almt12-1 were much lower and much larger than in the wild type. These results suggest that the anion channels are involved in the regulation of [Ca2+]cyt elevation in guard cells.

    DOI: 10.1093/bbb/zbab118

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  • Luteolin suppresses 5-hydroxytryptamine elevation in stimulated RBL-2H3 cells and experimental colitis mice. 査読

    Naoko Suga, Akira Murakami, Hideyuki Arimitsu, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoji Kato

    Journal of clinical biochemistry and nutrition   69 ( 1 )   20 - 27   2021年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Increased 5-hydroxytryptamine may be associated with the development and progression of inflammatory bowel disease. In this study, we examined the suppressive effect of flavonoids on the increased intra- and extracellular 5-hydroxytryptamine levels in rat mast RBL-2H3 cells, known to produce 5-hydroxytryptamine by the phorbol 12-myristate 13-acetate stimulation. Among the flavonoids examined, luteolin and quercetin significantly reduced the cellular 5-hydroxytryptamine concentration. Gene and protein expression analyses revealed that luteolin significantly suppressed cellular tryptophan hydroxylase 1 expression induced by phorbol 12-myristate 13-acetate stimulation. Mitogen-activated protein kinase/extracellular signal-regulated kinase signaling was also suppressed by luteolin, suggesting that this pathway is one of targets of 5-hydroxytryptamine modulation by luteolin. An in vivo experimental colitis model was prepared by administering 2.5% dextran sodium sulfate in drinking water to C57BL/6 mice for seven days. The ingestion of 0.1% dietary luteolin suppressed the increasing 5-hydroxytryptamine in the colorectal mucosa. In conclusion, luteolin possesses a suppressive effect on extensive 5-hydroxytryptamine formation in both experimental RBL-2H3 cells and colitis models.

    DOI: 10.3164/jcbn.20-192

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  • A multidrug resistance-associated protein inhibitor is a potential enhancer of the benzyl isothiocyanate-induced apoptosis induction in human colorectal cancer cells. 査読 国際誌

    Qifu Yang, Toshiyuki Nakamura, Masayuki Seto, Miku Miyagawa, Wensi Xu, Beiwei Zhu, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

    Journal of biochemical and molecular toxicology   35 ( 7 )   e22791   2021年7月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The increasing drug efflux through the ATP-binding cassette (ABC) transporters is the most plausible mechanism that mediates resistance to the anticancer phytochemicals, such as benzyl isothiocyanate (BITC), as well as chemotherapy drugs. To identify a potential component to overcome this resistance by combinatory utilization, we focused on multidrug resistance-associated proteins (MRPs) pumping various drug metabolites with glutathione as well as the organic anions. The pharmacological treatment of an MRP inhibitor, MK571, significantly potentiated the BITC-induced antiproliferation, coincided with the enhanced accumulation of BITC and glutathione in human colorectal cancer HCT-116 cells. MK571 also enhanced the apoptosis induction as well as activation of the mitogen-activated protein kinases and caspase-3, whereas it did not affect their basal levels. These results suggested that, since MRPs might play a pivotal role in the BITC efflux, MK571 potentiates the BITC-induced antiproliferation in human colorectal cancer cells through inhibition of the glutathione-dependent BITC efflux.

    DOI: 10.1002/jbt.22791

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  • Functional Evaluation of the Ethanol Extracts from Rosmarinus officinalis L. (Rosemary) 査読

    Yamada Naofumi, Yamasaki Tsutomu, Yamawaki Kana, Nakagiri Minami, Ito Hideyuki, Nakamura Yoshimasa, Nakanishi Tohru

    Journal of Hard Tissue Biology   30 ( 3 )   291 - 296   2021年

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    記述言語:英語   出版者・発行元:硬組織再生生物学会  

    <p>Rosemary is used as an herb in cooking and aromatic oils, and has long been used as a medicinal herb because of its functional components. According to folklore, monks recommended a remedy of rosemary in alcohol ("Hungarian water") to a Hungarian queen suffering from limb numbness, resulting in instant recovery and rejuvenation. This is why the queen married a 20-year-old king of Holland while in her seventies. In order to clarify the "rejuvenation" mechanisms of traditional Hungarian water, we examined the anti-oxidant activity, anti-glycation activity, inhibition of melanin production and suppression of tumor growth of ethanol extracts of rosemary in this study. It was found that the ethanol extract had high anti-oxidant activity, high anti-glycation activity, high inhibition of melanin production and high suppression of tumor cell growth.</p>

    DOI: 10.2485/jhtb.30.291

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  • PREDOMINANT RICE PHYTOCHEMICALS AND THEIR DISEASE-PREVENTIVE EFFECTS

    WU Hongyan, NAKAMURA Toshiyuki, NAKAMURA Yoshimasa

    Journal of Environmental Science for Sustainable Society (Web)   10 ( Supplement )   1 - 4   2021年

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    担当区分:責任著者  

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  • Stomatal response to isothiocyanates in Arabidopsis thaliana. 査読 国際誌

    Sonya Afrin, Eiji Okuma, Md Tahjib-Ul-Arif, Md Sarwar Jahan, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

    Journal of experimental botany   71 ( 22 )   6921 - 6931   2020年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Allyl isothiocyanate (AITC) induces stomatal closure accompanied by reactive oxygen species (ROS) production and glutathione (GSH) depletion in Arabidopsis thaliana. In this study, stomatal responses to three other isothiocyanates (ITCs), benzyl isothiocyanate (BITC), sulforaphane (SFN), and phenethyl isothiocyanate (PEITC), were investigated in A. thaliana. All these ITCs significantly induced stomatal closure, where PEITC and BITC were most effective. The selected ITCs also induced ROS accumulation, cytosolic alkalization, and GSH depletion in guard cells. Moreover, all ITCs increased the frequency of cytosolic free calcium ([Ca2+]cyt) spikes (transient elevation), while PEITC and BITC showed the highest frequency. There was a strong positive correlation between the number of [Ca2+]cyt spikes per guard cell and the decrease in stomatal aperture. Both cytosolic alkalization and GSH content have a positive correlation with the decrease in stomatal aperture, but ROS production did not have a significant correlation with the decrease in stomatal apertures. These results indicate that the molecules with a functional ITC group induce stomatal closure that is accompanied by GSH depletion, cytosolic alkalization, [Ca2+]cyt spikes, and ROS production, and that the former three cellular events, rather than ROS production, are highly correlated with the decrease in stomatal aperture.

    DOI: 10.1093/jxb/eraa420

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  • Fucoxanthin activities motivate its nano/micro-encapsulation for food or nutraceutical application: a review. 査読 国際誌

    Chunyan Wang, Xing Chen, Yoshimasa Nakamura, Chenxu Yu, Hang Qi

    Food & function   11 ( 11 )   9338 - 9358   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Fucoxanthin is a xanthophyll carotenoid abundant in marine brown algae. The potential therapeutic effects of fucoxanthin on tumor intervention have been well documented, which have aroused great interests in utilizing fucoxanthin in functional foods and nutraceuticals. However, the utilization of fucoxanthin as a nutraceutical in food and nutrient supplements is currently limited due to its low water solubility, poor stability, and limited bioaccessibility. Nano/micro-encapsulation is a technology that can overcome these challenges. A systematic review on the recent progresses in nano/micro-delivery systems to encapsulate fucoxanthin in foods or nutraceuticals is warranted. This article starts with a brief introduction of fucoxanthin and the challenges of oral delivery of fucoxanthin. Nano/micro-encapsulation technology is then covered, including materials and strategies for constructing the delivery system. Finally, future prospective has been discussed on properly designed oral delivery systems of fucoxanthin for managing cancer. Natural edible materials such as whey protein, casein, zein, gelatin, and starch have been successfully utilized to fabricate lipid-based, gel-based, or emulsion-based delivery systems, molecular nanocomplexes, and biopolymer nanoparticles with the aid of advanced processing techniques, such as freeze-drying, high pressure homogenization, sonication, anti-solvent precipitation, coacervation, ion crosslinking, ionic gelation, emulsification, and enzymatic conjugation. These formulated nano/micro-capsules have proven to be effective in stabilizing and enhancing the bioaccessibility of fucoxanthin. This review will inspire a surge of multidisciplinary research in a broader community of foods and motivate material scientists and researchers to focus on nano/micro-encapsulated fucoxanthin in order to facilitate the commercialization of orally-deliverable tumor intervention products.

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  • Exogenous proline enhances antioxidant enzyme activities but does not mitigate growth inhibition by selenate stress in tobacco BY-2 cells. 査読 国際誌

    Mousumi Khatun, Daiki Matsushima, Mohammad Saidur Rhaman, Eiji Okuma, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

    Bioscience, biotechnology, and biochemistry   84 ( 11 )   2281 - 2292   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Selenium (Se) causes oxidative damage to plants. Proline is accumulated as a compatible solute in plants under stress conditions and mitigates stresses. Selenate at 250 µM increased cell death and inhibited the growth of tobacco BY-2 cells while exogenous proline at 10 mM did not mitigate the inhibition by selenate. Selenate increased accumulation of Se and ROS and activities of antioxidant enzymes but not lipid peroxidation in the BY-2 cells. Proline increased Se accumulation and antioxidant enzyme activities but not either ROS accumulation or lipid peroxidation in the selenate-stressed cells. Glutathione (GSH) rather than ascorbic acid (AsA) mitigated the growth inhibition although both reduced the accumulation of ROS induced by selenate. These results indicate that proline increases both antioxidant enzyme activities and Se accumulation, which overall fails to ameliorate the growth inhibition by selenate and that the growth inhibition is not accounted for only by ROS accumulation. Abbreviations: APX: ascorbate peroxidase; AsA: ascorbic acid; BY-2: Bright Yellow-2; CAT: catalase; DAI: days after inoculation; DW: dry weight; FW: fresh weight; GSH: glutathione; ROS: reactive oxygen species.

    DOI: 10.1080/09168451.2020.1799747

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  • Reactive carbonyl species mediate methyl jasmonate-induced stomatal closure. 査読

    Md Moshiul Islam, Wenxiu Ye, Fahmida Akter, Mohammad Saidur Rhaman, Daiki Matsushima, Shintaro Munemasa, Eiji Okuma, Yoshimasa Nakamura, Md Sanaullah Biswas, Jun'ichi Mano, Yoshiyuki Murata

    Plant & cell physiology   2020年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Production of reactive oxygen species (ROS) is a key signal event for methyl jasmonate (MeJA)- and abscisic acid (ABA)-induced stomatal closure. We recently showed that reactive carbonyl species (RCS) stimulates stomatal closure as an intermediate downstream of hydrogen peroxide (H2O2) production in the ABA signaling pathway in guard cells of Nicotiana tabacum and Arabidopsis thaliana. In this study, we examined whether RCS functions as an intermediate downstream of H2O2 production in MeJA signaling in guard cells using transgenic tobacco plants overexpressing A. thaliana 2-alkenal reductase (n-alkanal + NAD(P)+ ⇌ 2-alkenal + NAD(P)H + H+) (AER-OE tobacco) and Arabidopsis plants. The stomatal closure induced by MeJA was impaired in the AER-OE tobacco and was inhibited by RCS scavengers, carnosine and pyridoxamine, in the wild-type (WT) tobacco plants and Arabidopsis plants. Application of MeJA significantly induced accumulation of RCS including acrolein and 4-hydroxy-(E)-2-nonenal in the WT tobacco but not in the AER-OE plants. Application of MeJA induced H2O2 production in the WT tobacco and the AER-OE plants and the H2O2 production was not inhibited by the RCS scavengers. These results suggest that RCS functions as an intermediate downstream of ROS production in MeJA signaling as well as in ABA signaling in guard cells.

    DOI: 10.1093/pcp/pcaa107

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  • Interaction of intracellular hydrogen peroxide accumulation with nitric oxide production in abscisic acid signaling in guard cells. 査読 国際誌

    Rayhanur Jannat, Takanori Senba, Daichi Muroyama, Misugi Uraji, Mohammad Anowar Hossain, Mohammad Muzahidul Islam, Yoshimasa Nakamura, Shintaro Munemasa, Izumi C Mori, Yoshiyuki Murata

    Bioscience, biotechnology, and biochemistry   84 ( 7 )   1418 - 1426   2020年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Reactive oxygen species and nitric oxide (NO•) concomitantly play essential roles in guard cell signaling. Studies using catalase mutants have revealed that the inducible and constitutive elevations of intracellular hydrogen peroxide (H2O2) have different roles: only the inducible H2O2 production transduces the abscisic acid (ABA) signal leading stomatal closure. However, the involvement of inducible or constitutive NO• productions, if exists, in this process remains unknown. We studied H2O2 and NO• mobilization in guard cells of catalase mutants. Constitutive H2O2 level was higher in the mutants than that in wild type, but constitutive NO• level was not different among lines. Induced NO• and H2O2 levels elicited by ABA showed a high correlation with each other in all lines. Furthermore, NO• levels increased by exogenous H2O2 also showed a high correlation with stomatal aperture size. Our results demonstrate that ABA-induced intracellular H2O2 accumulation triggers NO• production leading stomatal closure. ABBREVIATIONS: ABA: abscisic acid; CAT: catalase; cGMP: cyclic guanosine monophosphate; DAF-2DA: 4,5-diaminofluorescein-2 diacetate; H2DCF-DA: 2',7'-dichlorodihydrofluorescein diacetate; MeJA: methyljasmonate; NOS: nitric oxide synthetase; NR: nitrate reductase; POX: peroxidase; ROS: reactive oxygen species; SNAP: S-nitroso-N-acetyl-DL-penicillamine; SNP: sodium nitroprusside; NOX: NADP(H) oxidase.

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  • Inhibition of light-induced stomatal opening by allyl isothiocyanate does not require guard cell cytosolic Ca2+ signaling. 査読 国際誌

    Wenxiu Ye, Eigo Ando, Mohammad Saidur Rhaman, Md Tahjib-Ul-Arif, Eiji Okuma, Yoshimasa Nakamura, Toshinori Kinoshita, Yoshiyuki Murata

    Journal of experimental botany   71 ( 10 )   2922 - 2932   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The glucosinolate-myrosinase system is a well-known defense system that has been shown to induce stomatal closure in Brassicales. Isothiocyanates are highly reactive hydrolysates of glucosinolates, and an isothiocyanate, allyl isothiocyanate (AITC), induces stomatal closure accompanied by elevation of free cytosolic Ca2+ concentration ([Ca2+]cyt) in Arabidopsis. It remains unknown whether AITC inhibits light-induced stomatal opening. This study investigated the role of Ca2+ in AITC-induced stomatal closure and inhibition of light-induced stomatal opening. AITC induced stomatal closure and inhibited light-induced stomatal opening in a dose-dependent manner. A Ca2+ channel inhibitor, La3+, a Ca2+chelator, EGTA, and an inhibitor of Ca2+ release from internal stores, nicotinamide, inhibited AITC-induced [Ca2+]cyt elevation and stomatal closure, but did not affect inhibition of light-induced stomatal opening. AITC activated non-selective Ca2+-permeable cation channels and inhibited inward-rectifying K+ (K+in) channels in a Ca2+-independent manner. AITC also inhibited stomatal opening induced by fusicoccin, a plasma membrane H+-ATPase activator, but had no significant effect on fusicoccin-induced phosphorylation of the penultimate threonine of H+-ATPase. Taken together, these results suggest that AITC induces Ca2+ influx and Ca2+ release to elevate [Ca2+]cyt, which is essential for AITC-induced stomatal closure but not for inhibition of K+in channels and light-induced stomatal opening.

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  • The Myrosinases TGG1 and TGG2 Function Redundantly in Reactive Carbonyl Species Signaling in Arabidopsis Guard Cells. 査読

    Mohammad Saidur Rhaman, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

    Plant & cell physiology   61 ( 5 )   967 - 977   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Myrosinase (β-thioglucoside glucohydrolase, enzyme nomenclature, EC 3.2.1.147, TGG) is a highly abundant protein in Arabidopsis guard cells, of which TGG1 and TGG2 function redundantly in abscisic acid (ABA)- and methyl jasmonate-induced stomatal closure. Reactive carbonyl species (RCS) are α,β-unsaturated aldehydes and ketones, which function downstream of reactive oxygen species (ROS) production in the ABA signalling pathway in guard cells. Among the RCS, acrolein is the most highly reactive, which is significantly produced in ABA-treated guard cells. To clarify the ABA signal pathway downstream of ROS production, we investigated the responses of tgg mutants (tgg1-3, tgg2-1 and tgg1-3 tgg2-1) to acrolein. Acrolein induced stomatal closure and triggered cytosolic alkalization in wild type (WT), tgg1-3 single mutants and in tgg2-1 single mutants, but not in tgg1-3 tgg2-1 double mutants. Exogenous Ca2+ induced stomatal closure and cytosolic alkalization not only in WT but also in all of the mutants. Acrolein- and Ca2+-induced stomatal closures were inhibited by an intracellular acidifying agent, butyrate, a Ca2+ chelator, ethylene glycol tetraacetic acid (EGTA) and a Ca2+ channel blocker, LaCl3. Acrolein induced cytosolic free calcium concentration ([Ca2+]cyt) elevation in guard cells of WT plants but not in the tgg1-3 tgg2-1 double mutants. Exogenous Ca2+ elicited [Ca2+]cyt elevation in guard cells of WT and tgg1-3 tgg2-1. Our results suggest that TGG1 and TGG2 function redundantly, not between ROS production and RCS production, but downstream of RCS production in the ABA signal pathway in Arabidopsis guard cells.

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  • Salicylic acid receptor NPR1 is involved in guard cell chitosan signaling. 査読 国際誌

    Yeasin Prodhan, Mohammad Issak, Shintaro Munemasa, Yoshimasa Nakamura, Yoshiyuki Murata

    Bioscience, biotechnology, and biochemistry   84 ( 5 )   963 - 969   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Chitosan (CHT) induces stomatal closure and thus plays a crucial role in plants to adapt to the adverse environments. Our previous results of a SA-deficient mutant nahG suggest that endogenous salicylic acid (SA) is involved in the CHT signaling in guard cells. Here in order to make the involvement definite, we examined stomatal responses to CHT of another SA-deficient mutant, sid2, and an SA receptor mutant, npr1-3. The sid2 mutation impaired CHT-induced stomatal closure and reactive oxygen species production and both impairments were complemented with exogenous SA application. Moreover, the CHT-induced stomatal closure is disrupted in the npr1-3 mutant. These results suggest that endogenous SA is involved in the CHT-induced stomatal closure via the SA receptor, NPR1.Abbreviations: SA: salicylic acid; ABA: abscisic acid; ROS: reactive oxygen species; NPR1: nonexpresser of pathogenesis-related genes1; CHT: chitosan; DAB: 3,3'-diaminobenzidine.

    DOI: 10.1080/09168451.2020.1718485

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  • Synthesis and characterization of conductive flexible cellulose carbon nanohorn sheets for human tissue applications. 査読 国際誌

    Karthik Paneer Selvam, Taichi Nagahata, Kosuke Kato, Mayuko Koreishi, Toshiyuki Nakamura, Yoshimasa Nakamura, Takeshi Nishikawa, Ayano Satoh, Yasuhiko Hayashi

    Biomaterials research   24   18 - 18   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background: Conductive sheets of cellulose and carbon nanomaterials and its human skin applications are an interesting research aspect as they have potential for applications for skin compatibility. Hence it is needed to explore the effects and shed light on these applications. Method: To fabricate wearable, portable, flexible, lightweight, inexpensive, and biocompatible composite materials, carbon nanohorns (CNHs) and hydroxyethylcellulose (HEC) were used as precursors to prepare CNH-HEC (Cnh-cel) composite sheets. Cnh-cel sheets were prepared with different loading concentrations of CNHs (10, 20 50,100 mg) in 200 mg cellulose. To fabricate the bio-compatible sheets, a pristine composite of CNHs and HEC was prepared without any pretreatment of the materials. Results: The obtained sheets possess a conductivity of 1.83 × 10- 10 S/m and bio-compatible with human skin. Analysis for skin-compatibility was performed for Cnh-cel sheets by h-CLAT in vitro skin sensitization tests to evaluate the activation of THP-1 cells. It was found that THP-1 cells were not activated by Cnh-cel; hence Cnh-cel is a safe biomaterial for human skin. It was also found that the composite allowed only a maximum loading of 100 mg to retain the consistent geometry of free-standing sheets of < 100 μm thickness. Since CNHs have a unique arrangement of aggregates (dahlia structure), the composite is homogeneous, as verified by transmission electron microscopy (TEM) and, scanning electron microscopy (SEM), and other functional properties investigated by Raman spectroscopy, Fourier transform infrared spectroscopy (FT-IR), conductivity measurement, tensile strength measurement, and skin sensitization. Conclusion: It can be concluded that cellulose and CNHs sheets are conductive and compatible to human skin applications.

    DOI: 10.1186/s40824-020-00194-3

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  • Isolation and identification of zinc-chelating peptides from sea cucumber (Stichopus japonicus) protein hydrolysate. 査読

    Liu X, Wang Z, Zhang J, Song L, Li D, Wu Z, Zhu B, Nakamura Y, Shahidi F, Yu C, Zhou D

    Journal of the science of food and agriculture   99 ( 14 )   6400 - 6407   2019年11月

  • Dynamics of the Cellular Metabolism of Leptosperin Found in Manuka Honey. 査読 国際誌

    Yoji Kato, Masaki Kawai, Shota Kawai, Yayako Okano, Natsumi Rokkaku, Akari Ishisaka, Kaeko Murota, Toshiyuki Nakamura, Yoshimasa Nakamura, Shinichi Ikushiro

    Journal of agricultural and food chemistry   67 ( 39 )   10853 - 10862   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Leptosperin (methyl syringate β-d-gentiobioside) is abundantly found in manuka honey, which is widely used because of its antibacterial and possible anti-inflammatory activities. The aim of this study was to examine the molecular mechanism underlying the metabolism of leptosperin. Five phytochemicals (leptosperin, methyl syringate (MSYR), glucuronate conjugate of MSYR (MSYR-GA), sulfonate conjugate of MSYR (MSYR-S), and syringic acid (SYR)) were separately incubated with HepG2 and Caco-2 cells. After incubation, we found that the concentration of MSYR decreased, whereas the concentrations of SYR, MSYR-GA, and MSYR-S increased. By profiling with inhibitors and carboxylesterases (CES1, 2), we found that the conversion from MSYR to SYR was mediated by CES1. Lipopolysaccharide-stimulated RAW264.7 cells restored MSYR-GA to MSYR possibly by the secreted β-glucuronidase. All of the mice administered with leptosperin, MSYR, or manuka honey showed higher MSYR (13.84 ± 11.51, 14.29 ± 9.19, or 6.66 ± 2.30 nM) and SYR (1.85 ± 0.66, 6.01 ± 1.20, or 8.16 ± 3.10 nM) levels in the plasma compared with that of the vehicle controls (3.33 ± 1.45 (MSYR) and 1.85 ± 0.66 (SYR) nM). The findings of our study indicate that the unique metabolic pathways of these compounds may account for possible functionalities of manuka honey.

    DOI: 10.1021/acs.jafc.9b03894

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  • Ethylene Inhibits Methyl Jasmonate-Induced Stomatal Closure by Modulating Guard Cell Slow-Type Anion Channel Activity via the OPEN STOMATA 1/SnRK2.6 Kinase-Independent Pathway in Arabidopsis. 査読

    Munemasa S, Hirao Y, Tanami K, Mimata Y, Nakamura Y, Murata Y

    Plant & cell physiology   60 ( 10 )   2263 - 2271   2019年10月

  • Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides. 査読

    Liu X, Wang Z, Yin F, Liu Y, Qin N, Nakamura Y, Shahidi F, Yu C, Zhou D, Zhu B

    Marine drugs   17 ( 8 )   2019年7月

  • Reactive carbonyl species function as signal mediators downstream of H<inf>2</inf>O<inf>2</inf> production and regulate [Ca2+]<inf>cyt</inf> elevation in ABA signal pathway in Arabidopsis guard cells 査読

    Md Moshiul Islam, Wenxiu Ye, Daiki Matsushima, Mohammad Saidur Rhaman, Shintaro Munemasa, Eiji Okuma, Yoshimasa Nakamura, Md Sanaullah Biswas, Jun'ichi Mano, Yoshiyuki Murata

    Plant and Cell Physiology   60 ( 5 )   1146 - 1159   2019年5月

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    掲載種別:研究論文(学術雑誌)  

    © The Author(s) 2019. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. We have demonstrated that reactive carbonyl species (RCS) function as an intermediate downstream of hydrogen peroxide (H2O2) production in abscisic acid (ABA) signaling for stomatal closure in guard cells using transgenic tobacco plants overexpressing alkenal reductase. We investigated the conversion of the RCS production into downstream signaling events in the guard cells. Both ABA and H2O2 induced production of the RCS, such as acrolein and 4-hydroxy-(E)-2-nonenal (HNE), in epidermal tissues of wild-type Arabidopsis thaliana plants. Application of the RCS scavengers, carnosine and pyridoxamine, did not affect the ABA-induced H2O2 production but inhibited the ABA- and H2O2-induced stomatal closure. Both acrolein and HNE induced stomatal closure in a plasma membrane NAD(P)H oxidase mutant atrbohD atrbohF as well as in the wild type, but not in a calcium-dependent kinase mutant cpk6. Acrolein activated plasma membrane Ca2+-permeable cation channels, triggered cytosolic free Ca2+ concentration ([Ca2+]cyt) elevation, and induced stomatal closure accompanied by depletion of glutathione in the guard cells. These results suggest that RCS production is a signaling event between the ROS production and [Ca2+]cyt elevation during guard cell ABA signaling.

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  • Covalent modification of phosphatidylethanolamine by benzyl isothiocyanate and the resultant generation of ethanolamine adduct as its metabolite. 査読

    Nakamura T, Hirakawa M, Nakamura Y, Ishisaka A, Kitamoto N, Murakami A, Kato Y

    Chemical research in toxicology   2019年2月

  • The Golgin Protein Giantin Regulates Interconnections Between Golgi Stacks. 査読 国際誌

    Ayano Satoh, Mitsuko Hayashi-Nishino, Takuto Shakuno, Junko Masuda, Mayuko Koreishi, Runa Murakami, Yoshimasa Nakamura, Toshiyuki Nakamura, Naomi Abe-Kanoh, Yasuko Honjo, Joerg Malsam, Sidney Yu, Kunihiko Nishino

    Frontiers in cell and developmental biology   7 ( AUG )   160 - 160   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Golgins are a family of Golgi-localized long coiled-coil proteins. The major golgin function is thought to be the tethering of vesicles, membranes, and cytoskeletal elements to the Golgi. We previously showed that knockdown of one of the longest golgins, Giantin, altered the glycosylation patterns of cell surfaces and the kinetics of cargo transport, suggesting that Giantin maintains correct glycosylation through slowing down transport within the Golgi. Giantin knockdown also altered the sizes and numbers of mini Golgi stacks generated by microtubule de-polymerization, suggesting that it maintains the independence of individual Golgi stacks. Therefore, it is presumed that Golgi stacks lose their independence following Giantin knockdown, allowing easier and possibly increased transport among stacks and abnormal glycosylation. To gain structural insights into the independence of Golgi stacks, we herein performed electron tomography and 3D modeling of Golgi stacks in Giantin knockdown cells. Compared with control cells, Giantin-knockdown cells had fewer and smaller fenestrae within each cisterna. This was supported by data showing that the diffusion rate of Golgi membrane proteins is faster in Giantin-knockdown Golgi, indicating that Giantin knockdown structurally and functionally increases connectivity among Golgi cisternae and stacks. This increased connectivity suggests that contrary to the cis-golgin tether model, Giantin instead inhibits the tether and fusion of nearby Golgi cisternae and stacks, resulting in transport difficulties between stacks that may enable the correct glycosylation of proteins and lipids passing through the Golgi.

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  • Benzyl isothiocyanate ameliorates lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation. 査読

    Liang Y, Sasaki I, Takeda Y, Zhu B, Munemasa S, Nakamura T, Murata Y, Nakamura Y

    Bioscience, biotechnology, and biochemistry   82 ( 12 )   2130 - 2139   2018年12月

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    担当区分:最終著者, 責任著者  

    DOI: 10.1080/09168451.2018.1514247

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  • Oxidative stress involved in textural changes of sea cucumber Stichopus japonicus body wall during low-temperature treatment. 査読

    Dong X, Qi H, Feng D, He B, Nakamura Y, Yu C, Zhu B

    Int. J. Food Prop.   21 ( 1 )   2646 - 2659   2018年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Methyl-β-cyclodextrin potentiates the BITC-induced anti-cancer effect through modulation of the Akt phosphorylation in human colorectal cancer cells. 査読

    Yang Q, Miyagawa M, Liu X, Zhu B, Munemasa S, Nakamura T, Murata Y, Nakamura Y

    Bioscience, biotechnology, and biochemistry   82 ( 12 )   2158 - 2167   2018年12月

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    担当区分:最終著者, 責任著者  

    DOI: 10.1080/09168451.2018.1514249

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  • Lycii fructus extract ameliorates hydrogen peroxide-induced cytotoxicity through indirect antioxidant action. 査読

    Xu W, Saiki S, Myojin T, Liu Y, Zhu B, Murata Y, Ashida H, Tsunenaga M, Nakamura Y

    Bioscience, biotechnology, and biochemistry   82 ( 10 )   1812 - 1820   2018年10月

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    担当区分:最終著者, 責任著者  

    DOI: 10.1080/09168451.2018.1487274

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  • Guard Cell Salicylic Acid Signaling Is Integrated into Abscisic Acid Signaling via the Ca2+/CPK-Dependent Pathway. 査読

    Prodhan MY, Munemasa S, Nahar MN, Nakamura Y, Murata Y

    Plant physiology   178 ( 1 )   441 - 450   2018年9月

  • Lymphatic metabolites of quercetin after intestinal administration of quercetin-3-glucoside and its aglycone in rats 査読

    Toshiyuki Nakamura, Chinatsu Kinjo, Yoshimasa Nakamura, Yoji Kato, Miyu Nishikawa, Masahiro Hamada, Noriyuki Nakajima, Shinichi Ikushiro, Kaeko Murota

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   645   126 - 136   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Quercetin is a major flavonoid, present as its glycosidic forms in plant foods. In this study, quercetin-3-glucoside (Q3G) was administered intraduodenally to thoracic lymph-cannulated rats, and its lymphatic transport was investigated. The resulting lymphatic and plasma metabolites were identified with LC-MS/MS and compared with those after administration of quercetin aglycone.The total concentration of quercetin metabolites in the lymph was about four times lower than that in the plasma, and quercetin and its methylated form isorhamnetin were detected as their glucuronides, sulfates and diglucuronides both in the lymph and the plasma after Q3G and quercetin administrations. The lymph levels of the glucuronides after Q3G administration were lower than those after quercetin administration, whereas those in the plasma showed the opposite pattern. Both the lymph and plasma levels of the sulfates after Q3G administration were lower than those after quercetin administration. Some of the intestinal metabolites like quercetin monoglucuronides were transported directly into the lymph and the hepatic metabolites like the diglucuronides were eventually transferred from the plasma into the lymph.These results indicate that the absorbed Q3G is partly transported into the intestinal lymph as quercetin metabolites. Deglycosylation in the enterocyte is also suggested to affect the subsequent metabolic pathways.

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  • Benzyl isothiocyanate attenuates the hydrogen peroxide-induced interleukin-13 expression through glutathione S-transferase P induction in T lymphocytic leukemia cells. 査読

    Tang Y, Naito S, Abe-Kanoh N, Ogawa S, Yamaguchi S, Zhu B, Murata Y, Nakamura Y

    Journal of biochemical and molecular toxicology   e22054   2018年4月

  • Phenethyl isothiocyanate activates leptin signaling and decreases food intake. 査読 国際誌

    Miho Yagi, Yukiko Nakatsuji, Ayumi Maeda, Hiroki Ota, Ryosuke Kamikubo, Noriyuki Miyoshi, Yoshimasa Nakamura, Mitsugu Akagawa

    PloS one   13 ( 11 )   e0206748   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Obesity, a principal risk factor for the development of diabetes mellitus, heart disease, and hypertension, is a growing and serious health problem all over the world. Leptin is a weight-reducing hormone produced by adipose tissue, which decreases food intake via hypothalamic leptin receptors (Ob-Rb) and the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway. Protein tyrosine phosphatase 1B (PTP1B) negatively regulates leptin signaling by dephosphorylating JAK2, and the increased activity of PTP1B is implicated in the pathogenesis of obesity. Hence, inhibition of PTP1B may help prevent and reduce obesity. In this study, we revealed that phenethyl isothiocyanate (PEITC), a naturally occurring isothiocyanate in certain cruciferous vegetables, potently inhibits recombinant PTP1B by binding to the reactive cysteinyl thiol. Moreover, we found that PEITC causes the ligand-independent phosphorylation of Ob-Rb, JAK2, and STAT3 by inhibiting cellular PTP1B in differentiated human SH-SY5Y neuronal cells. PEITC treatment also induced nuclear accumulation of phosphorylated STAT3, resulting in enhanced anorexigenic POMC expression and suppressed orexigenic NPY/AGRP expression. We demonstrated that oral administration of PEITC to mice significantly reduces food intake, and stimulates hypothalamic leptin signaling. Our results suggest that PEITC might help prevent and improve obesity.

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  • (−)-Epigallocatechin-3-gallate inhibits human angiotensin-converting enzyme activity through an autoxidation-dependent mechanism 査読

    Zhe Liu, Sayaka Nakashima, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

    Journal of Biochemical and Molecular Toxicology   31 ( 9 )   2017年9月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    © 2017 Wiley Periodicals, Inc. We investigated the molecular mechanisms involved in the angiotensin-converting enzyme (ACE) inhibition by (−)-epigallocatechin-3-gallate (EGCg), a major tea catechin. EGCg inhibited both the ACE activity in the lysate of human colorectal cancer cells and human recombinant ACE (rh-ACE) in a dose-dependent manner. Co-incubation with zinc sulfate showed no influence on the rh-ACE inhibition by EGCg, whereas it completely counteracted the inhibitory effect of ethylenediaminetetraacetic acid, a chelating-type ACE inhibitor. Although hydrogen peroxide was produced by the autoxidation of EGCg, hydrogen peroxide itself had little effect on the ACE activity. Conversely, the co-incubation of EGCg with borate or ascorbic acid significantly diminished the EGCg inhibition. A redox-cycling staining experiment revealed that rh-ACE was covalently modified by EGCg. A Lineweaver–Burk plot analysis indicated that EGCg inhibited the ACE activity in a non-competitive manner. These results suggested that EGCg might allosterically inhibit the ACE activity through oxidative conversion into an electrophilic quinone.

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  • Inhibition of phosphatidylinositide 3-kinase ameliorates antiproliferation by benzyl isothiocyanate in human colon cancer cells 査読

    Xiaoyang Liu, Chiaki Takano, Tomomi Shimizu, Shintaro Yokobe, Naomi Abe-Kanoh, Beiwei Zhu, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   491 ( 1 )   209 - 216   2017年9月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    In the present study, we clarified the role of phosphatidylinositide 3-kinase (PI3K) in antiproliferation induced by benzyl isothiocyanate (BITC) in human colorectal cancer cells. BITC simultaneously activated the PI3K/Akt/forkhead box 0 (FoxO) pathway, whereas it significantly inhibited the proliferation in human colorectal cancer cells. Inhibitory experiments using a PI3K selective inhibitor, LY294002 or NVP-BEZ235, significantly enhanced the BITC-induced antiproliferation and apoptotic cell population with the attenuation of the BITC-induced activation of the PI3K/Akt/FoxO survival pathway. Furthermore, BITC enhanced the insulin-activated PI3K/Akt/FoxO pathway, possibly through its inhibition of the protein tyrosine phosphatase 1B enzymatic activity. Taken together, these results suggested that the PI3K/Akt/FoxO pathway negatively regulates the BITC-induced antiproliferation in human colorectal cancer cells. (C) 2017 Elsevier Inc. All rights reserved.

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  • Methylglyoxal induces inhibition of growth, accumulation of anthocyanin, and activation of glyoxalase I and II in Arabidopsis thaliana 査読

    Tahsina Sharmin Hoque, Misugi Uraji, Md Anamul Hoque, Yoshimasa Nakamura, Yoshiyuki Murata

    JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY   31 ( 7 )   2017年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Methylglyoxal (MG) is a highly reactive stress-related -ketoaldehyde and a physiological metabolite of glycolysis, which is accumulated in ample amount under stressful conditions. In the present study, the effect of different doses of MG on growth, anthocyanin production, MG contents, and activities of two types of glyoxalases (glyoxalase I and glyoxalase II) were examined in Arabidopsis seedlings. MG at 0.1 mM dose did not affect seedling growth, anthocyanin accumulation, MG contents, or activities of glyoxalases, whereas MG at 0.5 mM and 1 mM inhibited seedling growth and induced anthocyanin accumulation, MG accumulation, and glyoxalase (both I and II) activation. Therefore, MG can reduce plant growth as a toxic molecule and can stimulate stress responses as a signal molecule under stress conditions.

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  • Exogenous proline enhances the sensitivity of Tobacco BY-2 cells to arsenate 査読

    Mst Nur-E-Nazmun Nahar, Mohammad Muzahidul Islam, Md. Anamul Hoque, Anna Yonezawa, Md. Yeasin Prodhan, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   81 ( 9 )   1726 - 1731   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Arsenic causes physiological and structural disorders in plants. Proline is accumulated as a compatible solute in plants under various stress conditions and mitigates stresses. Here, we investigated the effects of exogenous proline on tobacco Bright Yellow-2 (BY-2) cultured cells under AsO4- stress. Arsenate did not inhibit BY-2 cell growth at 40 and 50 mu M but did it at 60 mu M. Proline at 0.5 to 10 mM did not affect the cell growth but delayed it at 20 mM. At 40 mu M AsO4-, neither 0.5 mM nor 1 mM proline affected the cell growth but 10 mM proline inhibited it. In the presence of AsO4-, 10 mM proline increased the number of Evans Blue-stained (dead) cells and decreased the number of total cells. Together, our results suggest that exogenous proline does not alleviate arsenate toxicity but enhances the sensitivity of BY-2 cells to arsenate.

    DOI: 10.1080/09168451.2017.1340088

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  • Chitosan signaling in guard cells requires endogenous salicylic acid 査読

    Md. Yeasin Prodhan, Mohammad Issak, Toshiyuki Nakamura, Shintaro Munemasa, Yoshimasa Nakamura, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   81 ( 8 )   1536 - 1541   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    An elicitor chitosan (CHT) induces stomatal closure but the mechanism remains to be clarified. A phytohormone salicylic acid (SA) is crucial for elicitor-induced defense signaling in plants. Here we investigated whether endogenous SA is required for CHT signaling in guard cells. In the SA-deficient nahG mutant, treatment of CHT did not induce either apoplastic reactive oxygen species (ROS) production or stomatal closure but co-treatment of CHT and SA induced both apoplastic ROS production and stomatal closure, indicating the involvement of endogenous SA in CHT-induced apoplastic ROS production and CHT-induced stomatal closure. Furthermore, CHT induced transient cytosolic free calcium concentration increments in the nahG mutant in the presence of exogenous SA but not in the absence of exogenous SA. These results provide evidence that endogenous SA is a crucial element in CHT-induced stomatal closure.

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  • Inhibition of phosphatidylinositide 3-kinase impairs the benzyl isothiocyanate-induced accumulation of autophagic molecules and Nrf2 in human colon cancer cells 査読

    Xiaoyang Liu, Naomi Abe-Kanoh, Yujia Liu, Beiwei Zhu, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   81 ( 11 )   2212 - 2215   2017年

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    The regulating role of phosphatidylinositide 3-kinase (PI3K) in benzyl isothiocyanate (BITC)-induced Nrf2 activation, contributing to the inducible expression of cytoprotective genes, was investigated. BITC significantly enhanced the accumulation of Nrf2 as well as autophagic molecules in human colorectal cancer HCT-116 cells. Experiments using a PI3K-specific inhibitor suggested that PI3K plays the key role in the non-canonical Nrf2 activation by BITC.

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  • Cytotoxic and cytoprotective effects of tryptamine-4,5-dione on neuronal cells: a double-edged sword 査読

    Naoko Suga, Akira Murakami, Yoshimasa Nakamura, Akari Ishisaka, Noritoshi Kitamoto, Mikiko Ito, Yoji Kato

    FREE RADICAL RESEARCH   51 ( 5 )   545 - 553   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Serotonin (5-hydroxytryptamine) is a putative substrate for myeloperoxidase, which may convert it into the reactive quinone tryptamine-4,5-dione (TD). In this study, we found that the viability of human SH-SY5Y neuroblastoma cells treated with 25 mu M TD was increased to approximately 117%. On the other hand, the cell viability was significantly decreased by exposure to TD (150-200 mu M), with an increase in intracellular reactive oxygen species (ROS). Interestingly, pretreatment of SH-SY5Y cells with 100 mu M TD prevented cell death and suppressed intracellular ROS generation evoked by the addition of hydrogen peroxide (H2O2). Expression of the phase-II antioxidant enzyme NAD(P)H: quinone oxidoreductase 1 and haem oxygenase 1 were upregulated by TD at a concentration of 50-100 mu M. Nuclear factor erythroid 2-related factor 2 (Nrf2), the regulator of these enzyme, was translocated from the cytosol to the nucleus by 100 mu M TD. In summary, moderate concentrations of TD may increase the self-defence capacity of neuronal cells against oxidative stress.

    DOI: 10.1080/10715762.2017.1331038

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  • MPK9 and MPK12 function in SA-induced stomatal closure in Arabidopsis thaliana 査読

    Md. Atiqur Rahman Khokon, Mohammad Abdus Salam, Fabien Jammes, Wenxiu Ye, Mohammad Anowar Hossain, Eiji Okuma, Yoshimasa Nakamura, Izumi C. Mori, June M. Kwak, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   81 ( 7 )   1394 - 1400   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) and methyl jasmonate (MeJA) signaling. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling and MeJA signaling in Arabidopsis thaliana. In this study, we examined whether these two MAPKs are involved in SA-induced stomatal closure using genetic mutants and a pharmacological, MAPKK inhibitor. Salicylic acid induced stomatal closure in mpk9 and mpk12 single mutants but not in mpk9 mpk12 double mutants. The MAPKK inhibitor PD98059 inhibited SA-induced stomatal closure in wild-type plants. Salicylic acid induced extracellular reactive oxygen species (ROS) production, intracellular ROS accumulation, and cytosolic alkalization in the mpk9, mpk12, and mpk9 mpk12 mutants. Moreover, SA-activated S-type anion channels in guard cells of wild-type plants but not in guard cells of mpk9 mpk12 double mutants. These results imply that MPK9 and MPK12 are positive regulators of SA signaling in Arabidopsis guard cells.

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  • 3,4-Dihydroxyphenylacetic acid is a potential aldehyde dehydrogenase inducer in murine hepatoma Hepa1c1c7 cells 査読

    Yujia Liu, Ayuki Kurita, Sayaka Nakashima, Beiwei Zhu, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   81 ( 10 )   1978 - 1983   2017年

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    3,4-Dihydroxyphenylacetic acid (DOPAC) is one of the major colonic microflora-produced catabolites of quercetin glycosides, such as quercetin 4-glucoside derived from onion. Here, we investigated whether DOPAC modulates the aldehyde dehydrogenase (ALDH) activity and protects the cells from the acetaldehyde-induced cytotoxicity in vitro. DOPAC was shown to enhance not only the total ALDH activity, but also the gene expression of ALDH1A1, ALDH2 and ALDH3A1 in a concentration-dependent manner. DOPAC simultaneously stimulated the nuclear translocation of NFE2-related factor 2 and aryl hydrocarbon receptor. The pretreatment of DOPAC completely protected the cells from the acetaldehyde-induced cytotoxicity. The present study suggested that DOPAC acts as a potential ALDH inducer to prevent the alcohol-induced abnormal reaction.

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  • Reactive Carbonyl Species Mediate ABA Signaling in Guard Cells 査読

    Md. Moshiul Islam, Wenxiu Ye, Daiki Matsushima, Shintaro Munemasa, Eiji Okuma, Yoshimasa Nakamura, Md. Sanaullah Biswas, Jun'ichi Mano, Yoshiyuki Murata

    PLANT AND CELL PHYSIOLOGY   57 ( 12 )   2552 - 2563   2016年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Drought is responsible for a massive reduction in crop yields. In response to drought, plants synthesize the hormone ABA, which induces stomatal closure, thus reducing water loss. In guard cells, ABA triggers production of reactive oxygen species (ROS), which is mediated by NAD(P) H oxidases. The production of ROS is a key factor for ABA-induced stomatal closure, but it remains to be clarified how the production of ROS is transduced into downstream signaling components in guard cells. We investigated roles of reactive carbonyl species (RCS) in ABA-induced stomatal closure using transgenic tobacco (Nicotiana tabacum) overexpressing Arabidopsis 2-alkenal reductase (AER-OE), which scavenges RCS. ABA and hydrogen peroxide (H2O2) induced accumulation of RCS including acrolein and 4-hydroxy-(E)-2-nonenal in wild-type tobacco but not in AER-OE. Stomatal closure and RCS accumulation in response to ABA and H2O2 were inhibited in AER-OE unlike in the wild type, while ABA-induced H2O2 production in guard cells was observed in AER-OE as well as in the wild type. Moreover, ABA inhibited inward-rectifying K+ channels in wild-type guard cells but not in AER-OE guard cells. These results suggest that RCS is involved in ABAinduced stomatal closure and functions downstream of H2O2 production in the ABA signaling pathway in guard cells.

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  • Galloylated Catechins as Potent Inhibitors of Angiotensin Converting Enzyme 査読

    Zhe Liu, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

    FOOD SCIENCE AND TECHNOLOGY RESEARCH   22 ( 6 )   847 - 851   2016年11月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    In the present study, we investigated the inhibitory effects of four tea catechins, including (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECg) and (-)-epigallocatechin gallate (EGCg), on angiotensin converting enzyme (ACE) activity in vitro. Each catechin treatment significantly reduced the ACE activity with the order of potency being EGCg &gt; ECg &gt; EGC = EC. The addition of 1 mM borate significantly recovered the reduced ACE activities by tea catechins, suggesting that hydroxyl groups at the B-ring or at a galloyl moiety play an important role in the ACE-inhibitory mechanism. The covalent modification of ACE by tea catechins was also observed by a redox-cycling staining experiment. A Lineweaver-Burk plot indicated that EGC and ECg were noncompetitive inhibitors. The galloylated catechins might more potently inhibit ACE activity in an allosteric manner through the interaction of the galloyl moiety with the non-catalytic site of ACE.

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  • A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite 査読

    Sayaka Nakashima, Zhe Liu, Yuya Yamaguchi, Shunya Saiki, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura

    Biochemistry and Biophysics Reports   7   240 - 245   2016年9月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier B.V.  

    3,4-Dihydroxyphenylacetic acid (DOPAC) is one of the colonic microflora-produced catabolites of quercetin 4'-glucoside (Q4'G). Although the interaction of DOPAC with cellular proteins might be involved in its biological activity, the actual proteins have not yet been identified. In this study, we developed a novel tag-free DOPAC probe to label the targeted proteins by the copper(I)-catalyzed azide alkyne cycloaddition (CuAAC) and verified its efficacy. Various labeled proteins were detected by the DOPAC probe with the azide labeled biotin and a horseradish peroxidase (HRP)-streptavidin complex. Furthermore, a pulldown assay identified Keap1 and aryl hydrocarbon receptor (AhR) as the target proteins for the phase 2 enzyme up-regulation.

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  • Involvement of OST1 Protein Kinase and PYR/PYL/RCAR Receptors in Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis Guard Cells 査読

    Ye Yin, Yuji Adachi, Yoshimasa Nakamura, Shintaro Munemasa, Izumi C. Mori, Yoshiyuki Murata

    PLANT AND CELL PHYSIOLOGY   57 ( 8 )   1779 - 1790   2016年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Methyl jasmonate (MeJA) induces stomatal closure. It has been shown that stomata of many ABA-insensitive mutants are also insensitive to MeJA, and a low amount of ABA is a prerequisite for the MeJA response. However, the molecular mechanisms of the interaction between ABA and MeJA signaling remain to be elucidated. Here we studied the interplay of signaling of the two hormones in guard cells using the quadruple ABA receptor mutant pyr1 pyl1 pyl2 pyl4 and ABA-activated protein kinase mutants ost1-2 and srk2e. In the quadruple mutant, MeJA-induced stomatal closure, H2O2 production, nitric oxide (NO) production, cytosolic alkalization and plasma membrane Ca2+-permeable current (I-Ca) activation were not impaired. At the same time, the inactivation of the inward-rectifying K+ current was impaired. In contrast to the quadruple mutant, MeJA-induced stomatal closure, H2O2 production, NO production and cytosolic alkalization were impaired in ost1-2 and srk2e as well as in aba2-2, the ABA-deficient mutant. The activation of ICa was also impaired in srk2e. Collectively, these results indicated that OST1 was essential for MeJA-induced stomatal closure, while PYR1, PYL1, PYL2 and PYL4 ABA receptors were not sufficient factors. MeJA did not appear to activate OST1 kinase activity. This implies that OST1 mediates MeJA signaling through an undetectable level of activity or a nonenzymatic action. MeJA induced the expression of an ABA synthesis gene, NCED3, and increased ABA contents only modestly. Taken together with previous reports, this study suggests that MeJA signaling in guard cells is primed by ABA and is not brought about through the pathway mediated by PYR1, PYL1 PYL2 and PYL4.

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  • Apoptosis induction is involved in UVA-induced autolysis in sea cucumber Stichopus japonicus 査読

    Hang Qi, Hui Fu, Xiufang Dong, Dingding Feng, Nan Li, Chengrong Wen, Yoshimasa Nakamura, Beiwei Zhu

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY   158   130 - 135   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE SA  

    Autolysis easily happens to sea cucumber (Stichopus japonicus, S. japonicus) for external stimulus like UV exposure causing heavy economic losses. Therefore, it is meaningful to reveal the mechanism of S. japonicas autolysis. In the present study, to examine the involvement of apoptosis induction in UVA-induced autolysis of S. japonicas, we investigated the biochemical events including the DNA fragmentation, caspase-3 activation, mitogen-activated protein kinases (MAPKs) phosphorylation and free radical formation. Substantial morphological changes such as intestine vomiting and dermatolysis were observed in S. japonicus during the incubation after 1-h UVA irradiation (10 W/m(2)). The degradation of the structural proteins and enhancement of cathepsin L activity were also detected, suggesting the profound impact of proteolysis caused by the UVA irradiation even for 1 h. Furthermore, the DNA fragmentation and specific activity of caspase-3 was increased up to 12 h after UVA irradiation. The levels of phosphorylated p38 mitogen activated protein kinase (MAPK) and phosphorylated c-jun.-N-terminal kinase (JNK) were significantly increased by the UVA irradiation for 1 h. An electron spin resonance (ESR) analysis revealed that UVA enhanced the free radical formation in S. japonicas, even through we could not identify the attributed species. These results suggest that UVA-induced autolysis in S. japonicas at least partially involves the oxidative stress-sensitive apoptosis induction pathway. These data present a novel insight into the mechanisms of sea cucumber autolysis induced by external stress. (C) 2016 Elsevier B.V. All rights reserved.

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  • A mushroom-derived amino acid, ergothioneine, is a potential inhibitor of inflammation-related DNA halogenation 査読

    Takashi Asahi, Xiaohong Wu, Hiroshi Shimoda, Shinsuke Hisaka, Etsuko Harada, Tomomi Kanno, Yoshimasa Nakamura, Yoji Kato, Toshihiko Osawa

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   80 ( 2 )   313 - 317   2016年2月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Myeloperoxidase (MPO)-generated halogenating molecules, such as hypochlorous acid and hypobromous acid (HOBr), in inflammatory regions are postulated to contribute to disease progression. In this study, we showed that ergothioneine (EGT), derived from an edible mushroom, inhibited MPO activity as well as the formation of 8-bromo-2-deoxyguanosine in vitro. The HOBr scavenging effect of EGT is higher than those of ascorbic acid and glutathione. We initially observed that the administration of Coprinus comatus, an edible mushroom containing a high amount of EGT, inhibited the UV-B-induced inflammatory responses and DNA halogenation, suggesting that EGT is a promising anti-inflammatory agent from mushrooms.

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  • アブラナ科野菜漬物(カブ,ハクサイ)のイソチオシアネート生成に関する塩化ナトリウム(NaCl)およびアスコルビン酸の影響 査読

    宮澤 紀子, 阿部 雅子, 木村 典代, 松岡 寛樹, 田中 進, 森光 康次郎, 中村 宜督, 綾部 園子, 小澤 好夫

    日本調理科学会誌   49 ( 2 )   138 - 146   2016年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本調理科学会  

    野菜を常に摂取すると生活習慣病のリスクを軽減することが知られている。日本では,グルコシノレートを含む多くのアブラナ科野菜が漬物や煮物さらに浅漬けとして消費されている。グルコシノレートは組織が損傷するとミロシナーゼにより独特なフレーバーと発がん抑制作用を有するイソチオシアネートに加水分解される。 NaClとアスコルビン酸(VC)がミロシナーゼ活性に与える影響について検討した結果,1%NaClと0.1%VC濃度が最もミロシナーゼ活性を賦活した。一方,NaClとVCの共存においては,活性は抑制されたが,その抑制率はVC濃度が増加するに従い減少した。 さらに,漬物中のイソチオシアネート生成へのNaClの影響を検討するために,1, 2および3%NaCl濃度のカブとハクサイの浅漬けを調製した。GC-MS分析により3-ブテニル,4-ペンテニル,フェネチルイソチオシアネートを検出した。これらは,カブ,ハクサイ共に3%NaCl濃度で最も多く生成し,0.4%VCの添加は,1.4~1.6倍イソチオシアネートの生成を増大させた。官能評価により2%NaCl濃度における浅漬けが好まれた。

    DOI: 10.11402/cookeryscience.49.138

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  • Plant Polyphenols as a Double-Edged Sword in Health Promotion : Lessons from the Experimental Models Using Simple Phenolic Acids 招待 査読

    Nakamura Yoshimasa

    Agri-bioscience monographs AGBM   6 ( 1 )   1 - 57   2016年

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    担当区分:筆頭著者, 最終著者, 責任著者   記述言語:英語   出版者・発行元:Terrapub  

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  • Specific role of taurine in the 8-brominated-2′-deoxyguanosine formation 査読

    Takashi Asahi, Takashi Asahi, Yoshimasa Nakamura, Yoji Kato, Toshihiko Osawa

    Archives of Biochemistry and Biophysics   586   45 - 50   2015年11月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    © 2015 Elsevier Inc. All rights reserved. At the sites of inflammation, hypohalous acids, such as hypochlorous acid and hypobromous acid (HOBr), are produced by myeloperoxidase. These hypohalous acids rapidly react with the primary amino groups to produce haloamines, which are relatively stable and can diffuse long distances and cross the plasma membrane. In this study, we examined the effects of taurine, the most abundant free amino acid in the leukocyte cytosol, on the hypohalous acid-dependent formation of 8-chloro-2′-deoxyguanosine (8-CldG) and 8-bromo-2′-deoxyguanosine (8-BrdG). The reaction of taurine with HOBr yielded taurine bromamine, which is the most stable among other bromamines of amino acids. Taurine also enhanced the bromination of only dG among the four 2′-deoxynucleosides, whereas it inhibited the 8-CldG formation. The specificity of taurine for the enhanced formation of halogenated dG is completely different from that of nicotine, an enhancer of chlorination. The amount of dibrominated taurine (taurine dibromamine) closely correlated with the formation of 8-BrdG, suggesting that taurine dibromamine might be a plausible mediator for the dG bromination in vivo.

    DOI: 10.1016/j.abb.2015.10.002

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  • Allyl isothiocyanate induces stomatal closure in Vicia faba 査読

    Muhammad Abdus Sobahan, Nasima Akter, Eiji Okuma, Misugi Uraji, Wenxiu Ye, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   79 ( 10 )   1737 - 1742   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Isothiocyanates are enzymatically produced from glucosinolates in plants, and allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis thaliana. In this study, we investigated stomatal responses to AITC in Vicia faba. AITC-induced stomatal closure accompanied by reactive oxygen species (ROS) and NO production, cytosolic alkalization and glutathione (GSH) depletion in V. faba. GSH monoethyl ester induced stomatal reopening and suppressed AITC-induced GSH depletion in guard cells. Exogenous catalase and a peroxidase inhibitor, salicylhydroxamic acid, inhibited AITC-induced stomatal closure, unlike an NAD(P)H oxidase inhibitor, diphenylene iodonium chloride. The peroxidase inhibitor also abolished the AITC-induced ROS production, NO production, and cytosolic alkalization. AITC-induced stomatal closure was suppressed by an NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, and an agent to acidify cytosol, butyrate. These results indicate that AITC-induced stomatal closure in V. faba as well as in A. thaliana and suggest that AITC signaling in guard cells is conserved in both plants.

    DOI: 10.1080/09168451.2015.1045827

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  • Two guard cell mitogen-activated protein kinases, MPK9 and MPK12, function in methyl jasmonate-induced stomatal closure in Arabidopsis thaliana 査読

    Md. A. R. Khokon, M. A. Salam, F. Jammes, W. Ye, M. A. Hossain, M. Uraji, Y. Nakamura, I. C. Mori, J. M. Kwak, Y. Murata

    PLANT BIOLOGY   17 ( 5 )   946 - 952   2015年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Methyl jasmonate (MeJA) and abscisic acid (ABA) signalling cascades share several signalling components in guard cells. We previously showed that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signalling in Arabidopsis thaliana. In this study, we examined whether these two MAP kinases function in MeJA signalling using genetic mutants for MPK9 and MPK12 combined with a pharmacological approach. MeJA induced stomatal closure in mpk9-1 and mpk12-1 single mutants as well as wild-type plants, but not in mpk9-1 mpk12-1 double mutants. Consistently, the MAPKK inhibitor PD98059 inhibited the MeJA-induced stomatal closure in wild-type plants. MeJA elicited reactive oxygen species (ROS) production and cytosolic alkalisation in guard cells of the mpk9-1, mpk12-1 and mpk9-1 mpk12-1 mutants, as well in wild-type plants. Furthermore, MeJA triggered elevation of cytosolic Ca2+ concentration ([Ca2+](cyt)) in the mpk9-1 mpk12-1 double mutant as well as wild-type plants. Activation of S-type anion channels by MeJA was impaired in mpk9-1 mpk12-1. Together, these results indicate that MPK9 and MPK12 function upstream of S-type anion channel activation and downstream of ROS production, cytosolic alkalisation and [Ca2+](cyt) elevation in guard cell MeJA signalling, suggesting that MPK9 and MPK12 are key regulators mediating both ABA and MeJA signalling in guard cells.

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  • Open Stomata 1 Kinase is Essential for Yeast Elicitor-Induced Stomatal Closure in Arabidopsis 査読

    Wenxiu Ye, Yuji Adachi, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    PLANT AND CELL PHYSIOLOGY   56 ( 6 )   1239 - 1248   2015年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    We recently demonstrated that yeast elicitor (YEL)-induced stomatal closure requires a Ca2+-dependent kinase, CPK6. A Ca2+-independent kinase, Open Stomata 1 (OST1), is involved in stomatal closure induced by various stimuli including ABA. In the present study, we investigated the role of OST1 in YEL-induced stomatal closure in Arabidopsis using a knock-out mutant, ost1-3, and a kinase-deficient mutant, ost1-2. YEL did not induce stomatal closure or activation of guard cell S-type anion channels in the ost1 mutants unlike in wild-type plants. However, YEL did not increase OST1 kinase activity in wild-type guard cells. The YEL-induced stomatal closure and activation of S-type anion channels were also impaired in a gain-of-function mutant of a clade A type 2C protein phosphatase (ABA INSENSITIVE 1), abi1-1C. In the ost1 mutants like in the wild type, YEL induced H2O2 accumulation, activation of non-selective Ca2+-permeable cation (I-Ca) channels and transient elevations in cytosolic free Ca2+ concentration ([Ca2+](cyt)) in guard cells. These results suggest that OST1 kinase is essential for stomatal closure and activation of S-type anion channels induced by YEL and that OST1 is not involved in H2O2 accumulation, I-Ca channel activation or [Ca2+](cyt) elevations in guard cells induced by YEL.

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  • Benzyl isothiocyanate inhibits IL-13 expression in human basophilic KU812 cells 査読

    Yue Tang, Naomi Abe, Motoko Yoshimoto, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   79 ( 1 )   159 - 163   2015年1月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    An allergen-stimulating cytokine, interleukin-13 (IL-13), plays a significant role in allergic inflammation. Benzyl isothiocyanate (BITC), derived from several cruciferous vegetables, significantly suppressed the IL-13 expression in the calcium ionophore-stimulated human basophilic KU812 cells. Down-regulation of phosphorylated mitogen-activated protein kinases as well as nuclear transcriptional factors might be involved in the underlying mechanism.

    DOI: 10.1080/09168451.2014.963503

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  • Inhibition by acrolein of light-induced stomatal opening through inhibition of inward-rectifying potassium channels in Arabidopsis thaliana 査読

    Md. Moshiul Islam, Wenxiu Ye, Daiki Matsushima, Md. Atiqur Rahman Khokon, Shintaro Munemasa, Yoshimasa Nakamura, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   79 ( 1 )   59 - 62   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Acrolein is a reactive alpha,beta-unsaturated aldehyde derived from lipid peroxides, which are produced in plants under a variety of stress. We investigated effects of acrolein on light-induced stomatal opening using Arabidopsis thaliana. Acrolein inhibited light-induced stomatal opening in a dose-dependent manner. Acrolein at 100 mu M inhibited plasma membrane inward-rectifying potassium (K-in) channels in guard cells. Acrolein at 100 mu M inhibited K-in channel KAT1 expressed in a heterologous system using Xenopus leaves oocytes. These results suggest that acrolein inhibits light-induced stomatal opening through inhibition of K-in channels in guard cells.

    DOI: 10.1080/09168451.2014.951028

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  • Cyclic adenosine 5 '-diphosphoribose (cADPR) cyclic guanosine 3 ',5 '-monophosphate positively function in Ca2+ elevation in methyl jasmonate-induced stomatal closure, cADPR is required for methyl jasmonate-induced ROS accumulation NO production in guard cells 査読

    M. A. Hossain, W. Ye, S. Munemasa, Y. Nakamura, I. C. Mori, Y. Murata

    PLANT BIOLOGY   16 ( 6 )   1140 - 1144   2014年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Methyl jasmonate (MeJA) signalling shares several signal components with abscisic acid (ABA) signalling in guard cells. Cyclic adenosine 5-diphosphoribose (cADPR) and cyclic guanosine 3,5-monophosphate (cGMP) are second messengers in ABA-induced stomatal closure. In order to clarify involvement of cADPR and cGMP in MeJA-induced stomatal closure in Arabidopsis thaliana (Col-0), we investigated effects of an inhibitor of cADPR synthesis, nicotinamide (NA), and an inhibitor of cGMP synthesis, LY83583 (LY, 6-anilino-5,8-quinolinedione), on MeJA-induced stomatal closure. Treatment with NA and LY inhibited MeJA-induced stomatal closure. NA inhibited MeJA-induced reactive oxygen species (ROS) accumulation and nitric oxide (NO) production in guard cells. NA and LY suppressed transient elevations elicited by MeJA in cytosolic free Ca2+ concentration ([Ca2+](cyt)) in guard cells. These results suggest that cADPR and cGMP positively function in [Ca2+](cyt) elevation in MeJA-induced stomatal closure, are signalling components shared with ABA-induced stomatal closure in Arabidopsis, and that cADPR is required for MeJA-induced ROS accumulation and NO production in Arabidopsis guard cells.

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  • Tea Catechins Inhibit Cell Proliferation Through Hydrogen Peroxide-Dependent and -Independent Pathways in Human T lymphocytic Leukemia Jurkat Cells 査読

    Yue Tang, Naomi Abe, Hang Qi, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

    FOOD SCIENCE AND TECHNOLOGY RESEARCH   20 ( 6 )   1245 - 1249   2014年11月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    In the present study, we investigated the anti-proliferative effects of four tea catechins: (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECg) and (-)-epigallocatechin gallate (EGCg) in human T lymphocytic leukemia Jurkat cells. Each catechin induced a significant cytotoxic effect on Jurkat cells. Combinations of EGCg with other catechins additively potentiated anti-proliferation, extracellular hydrogen peroxide formation, c-Jun N-terminal kinase (JNK) activation and interferon (IFN)-gamma mRNA expression. Catalase partly but significantly abolished the cytotoxicity induced by EGC or EGCg, whereas it did not influence the EC- or ECg-induced effect. Among the tea catechins, ECg synergistically enhanced the EGCg-induced JNK phosphorylation and IFN-gamma expression. The present findings provide evidence that tea catechins are able to concertedly induce cytotoxicity through the JNK/IFN-gamma pathway in both a hydrogen peroxide-dependent and -independent manner.

    DOI: 10.3136/fstr.20.1245

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  • (-)-Epigallocatechin-3-Gallate Ameliorates Photodynamic Therapy Responses in an In Vitro T Lymphocyte Model 査読

    Hang Qi, Naomi Abe, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

    PHYTOTHERAPY RESEARCH   28 ( 10 )   1486 - 1491   2014年10月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    (-)-Epigallocatechin-3-gallate (EGCG), the most abundant polyphenolic constituent in green tea, is known as a powerful antioxidant but concomitantly possesses a prooxidant property. We investigated the effect of EGCG on phloxine B (PhB)-induced photocytotoxicity in human T lymphocytic leukemia Jurkat cells. EGCG significantly potentiated PhB-induced photocytotoxic effects, including the inhibition of cell proliferation, DNA fragmentation, and caspase-3 activity induction in Jurkat cells. Catalase attenuated the enhanced cytotoxicity by EGCG, suggesting the involvement of extracellularly produced hydrogen peroxide. Indeed, EGCG significantly enhanced extracellular hydrogen peroxide formation induced by photo-irradiated PhB. The EGCG also enhanced intracellular reactive oxygen species accumulation, c-Jun N-terminal kinase (JNK) phosphorylation, and interferon- (IFN-) gene expression, all of which are involved in PhB-induced apoptosis. Taken together, our data suggest that EGCG is capable of potentiating photodynamic therapy responses, presumably through the intracellular oxidative stress-sensitive JNK/IFN- pathway by exogenous hydrogen peroxide formation. Copyright (c) 2014 John Wiley & Sons, Ltd.

    DOI: 10.1002/ptr.5152

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  • Thiol modification by bioactivated polyphenols and its potential role in skin inflammation 査読

    Yoshimasa Nakamura, Takeshi Ishii, Naomi Abe, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   78 ( 6 )   1067 - 1070   2014年6月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    In the present study, we evaluated the modifying behavior of simple phenolic compounds on the sulfhydryl groups of glutathione and proteins. The catechol-type polyphenols, including protocatechuic acid, but neither the monophenols nor O-methylated catechol, can modify the sulfhydryl groups in a phenol oxidase-dependent manner. The possible involvement of polyphenol bioactivation in the enhancement of skin inflammation was also suggested.

    DOI: 10.1080/09168451.2014.905190

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  • Ascorbic Acid Synergistically Potentiates Phloxine B-induced Photocytotoxicity in Human Acute Promyelocytic Leukemia Cells 査読

    Hang Qi, Qian Wu, Naomi Abe, Shunya Saiki, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

    JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY   28 ( 4 )   167 - 173   2014年4月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Ascorbic acid (AsA) is known as an antioxidant but concomitantly possesses a pro-oxidant property. Because the impact of AsA on photodynamic therapy response is unclear, we investigated the effect of AsA on photocytotoxicity induced by phloxine B in human acute promyelocytic leukemia HL-60 cells. AsA synergistically enhanced phloxine B-induced photocytotoxic effects, including inhibition of cell proliferation, DNA ladder formation, and caspase-3 activation, whereas AsA itself showed no photocytotoxicity. AsA also enhanced the consumption of the reduced glutathione level compared with the cells treated with phloxine B alone under the light condition. Combination of AsA with phloxine B under the light condition enhanced the phosphorylation of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase (MAPK). These effects were completely cancelled by catalase. These results suggest that AsA synergistically enhances phloxine B-induced photocytotoxicity, possibly through the extracellular oxidative stress-dependent MAPK pathway activation.

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  • Regulation of reactive oxygen species-mediated abscisic acid signaling in guard cells and drought tolerance by glutathione

    Shintaro Munemasa, Daichi Muroyama, Hiroki Nagahashi, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    Frontiers in Plant Science   4   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Frontiers Research Foundation  

    The phytohormone abscisic acid (ABA) induces stomatal closure in response to drought stress, leading to reduction of transpirational water loss. A thiol tripeptide glutathione (GSH) is an important regulator of cellular redox homeostasis in plants. Although it has been shown that cellular redox state of guard cells controls ABA-mediated stomatal closure, roles of GSH in guard cell ABA signaling were largely unknown. Recently we demonstrated that GSH functions as a negative regulator of ABA signaling in guard cells. In this study we performed more detailed analyses to reveal how GSH regulates guard cell ABA signaling using the GSH-deficient Arabidopsis mutant cad2-1. The cad2-1 mutant exhibited reduced water loss from rosette leaves. Whole-cell current recording using patch clamp technique revealed that the cad2-1 mutation did not affect ABA regulation of S-type anion channels. We found enhanced activation of Ca2+ permeable channels by hydrogen peroxide (H2O2) in cad2-1 guard cells. The cad2-1 mutant showed enhanced H2O2-induced stomatal closure and significant increase of ROS accumulation in whole leaves in response to ABA. Our findings provide a new understanding of guard cell ABA signaling and a new strategy to improve plant drought tolerance. © 2013 Munemasa, Muroyama, Nagahashi, Nakamura, Mori and Murata.

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  • Erratum: Role of protein tyrosine phosphatase 1B in vascular endothelial growth factor signaling and cell-cell adhesions in endothelial cells (Circulation Research (2008) 102 (1182-1191) DOI: 10.1161/ CIRCRESAHA.107. 167080)

    Y. Nakamura, N. Patrushev, H. Inomata, D. Mehta, N. Urao, H. W. Kim, M. Razvi, V. Kini, K. Mahadev, B. J. Goldstein, R. McKinney, T. Fukai, M. Ushi-Fukai

    Circulation Research   113   2013年10月

  • Calcium-dependent protein Kinase CPK6 positively functions in induction by yeast elicitor of stomatal closure and inhibition by yeast elicitor of light-induced stomatal opening in Arabidopsis

    Wenxiu Ye, Daichi Muroyama, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    Plant Physiology   163 ( 2 )   591 - 599   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Yeast elicitor (YEL) induces stomatal closure that is mediated by a Ca2+-dependent signaling pathway. A Ca2+-dependent protein kinase, CPK6, positively regulates activation of ion channels in abscisic acid and methyl jasmonate signaling, leading to stomatal closure in Arabidopsis (Arabidopsis thaliana). YEL also inhibits light-induced stomatal opening. However, it remains unknown whether CPK6 is involved in induction by YEL of stomatal closure or in inhibition by YEL of light-induced stomatal opening. In this study, we investigated the roles of CPK6 in induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening in Arabidopsis. Disruption of CPK6 gene impaired induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening. Activation by YEL of nonselective Ca2+-permeable cation channels was impaired in cpk6-2 guard cells, and transient elevations elicited by YEL in cytosolic-free Ca2+ concentration were suppressed in cpk6-2 and cpk6-1 guard cells. YEL activated slow anion channels in wild-type guard cells but not in cpk6-2 or cpk6-1 and inhibited inward-rectifying K+ channels in wild-type guard cells but not in cpk6-2 or cpk6-1. The cpk6-2 and cpk6-1 mutations inhibited YEL-induced hydrogen peroxide accumulation in guard cells and apoplast of rosette leaves but did not affect YEL-induced hydrogen peroxide production in the apoplast of rosette leaves. These results suggest that CPK6 positively functions in induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening in Arabidopsis and is a convergent point of signaling pathways for stomatal closure in response to abiotic and biotic stress. © 2013 American Society of Plant Biologists. All Rights Reserved.

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  • Difference in Abscisic Acid Perception Mechanisms between Closure Induction and Opening Inhibition of Stomata

    Ye Yin, Yuji Adachi, Wenxiu Ye, Maki Hayashi, Yoshimasa Nakamura, Toshinori Kinoshita, Izumi C. Mori, Yoshiyuki Murata

    PLANT PHYSIOLOGY   163 ( 2 )   600 - 610   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Abscisic acid (ABA) induces stomatal closure and inhibits light-induced stomatal opening. The mechanisms in these two processes are not necessarily the same. It has been postulated that the ABA receptors involved in opening inhibition are different from those involved in closure induction. Here, we provide evidence that four recently identified ABA receptors (PYRABACTIN RESISTANCE1 [PYR1], PYRABACTIN RESISTANCE-LIKE1 [PYL1], PYL2, and PYL4) are not sufficient for opening inhibition in Arabidopsis (Arabidopsis thaliana). ABA-induced stomatal closure was impaired in the pyr1/pyl1/pyl2/pyl4 quadruple ABA receptor mutant. ABA inhibition of the opening of the mutant's stomata remained intact. ABA did not induce either the production of reactive oxygen species and nitric oxide or the alkalization of the cytosol in the quadruple mutant, in accordance with the closure phenotype. Whole cell patch-clamp analysis of inward-rectifying K+ current in guard cells showed a partial inhibition by ABA, indicating that the ABA sensitivity of the mutant was not fully impaired. ABA substantially inhibited blue light-induced phosphorylation of H+-ATPase in guard cells in both the mutant and the wild type. On the other hand, in a knockout mutant of the SNF1-related protein kinase, srk2e, stomatal opening and closure, reactive oxygen species and nitric oxide production, cytosolic alkalization, inward-rectifying K+ current inactivation, and H+-ATPase phosphorylation were not sensitive to ABA.

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  • Endogenous abscisic acid is involved in methyl jasmonate-induced reactive oxygen species and nitric oxide production but not in cytosolic alkalization in Arabidopsis guard cells

    Wenxiu Ye, Mohammad Anowar Hossain, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    JOURNAL OF PLANT PHYSIOLOGY   170 ( 13 )   1212 - 1215   2013年9月

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    記述言語:英語   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    We recently demonstrated that endogenous abscisic acid (ABA) is involved in methyl jasmonate (MeJA)induced stomatal closure in Arabidopsis thaliana. In this study, we investigated whether endogenous ABA is involved in MeJA-induced reactive oxygen species (ROS) and nitric oxide (NO) production and cytosolic alkalization in guard cells using an ABA-deficient Arabidopsis mutant, aba2-2, and an inhibitor of ABA biosynthesis, fiuridon (FLU). The aba2-2 mutation impaired MeJA-induced ROS and NO production. FLU inhibited MeJA-induced ROS production in wild-type guard cells. Pretreatment with 0.1 mu M ABA, which does not induce stomatal closure in the wild type, complemented the insensitivity to MeJA of the aba2-2 mutant. However, MeJA induced cytosolic alkalization in both wild-type and aba2-2 guard cells. These results suggest that endogenous ABA is involved in MeJA-induced ROS and NO production but not in MeJA-induced cytosolic alkalization in Arabidopsis guard cells. (C) 2013 Elsevier GmbH. All rights reserved.

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  • Catalases CAT1 and CAT3 Are not Key Enzymes in Alleviating Gamma Irradiation-Induced DNA Damage, H2O2 Accumulation, or Lipid Peroxidation in Arabidopsis thaliana

    Amena Sultana, Ikuko Minami, Daiki Matsushima, Mohammad Issak, Yoshimasa Nakamura, Setsuko Todoriki, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   77 ( 9 )   1984 - 1987   2013年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Gamma irradiation increased catalase activities at 0.1 kGy and decreased them at 10 kGy in Arabidopsis wild type and catalase-deficient mutants, cat3-1 and cat1 cat3. Irradiation induced DNA damage, H2O2 accumulation, and lipid peroxidation in both mutants as well as the wild type. Thus catalases might not be key enzymes protecting gamma irradiation-induced damage.

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  • Lower Photostability of Capsanthin Dispersed in an Aqueous Solution

    Masayuki Nishino, Makoto Sakata, Yoshiyuki Murata, Yoshimasa Nakamura

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   77 ( 6 )   1313 - 1316   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Food-additive grades of capsanthin, lutein, lycopene, and beta-carotene dispersed in aqueous solutions were photo-irradiated using a Xenon weather meter, and the levels of carotenoids were measured by HPLC and the absorbance method. Capsanthin photo-degraded more rapidly than the carotenoids tested, with less oxygen consumption. Unlike carotenes, capsanthin was partially converted into analogous colored compounds during degradation.

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  • Neither Endogenous Abscisic Acid nor Endogenous Jasmonate Is Involved in Salicylic Acid-, Yeast Elicitor-, or Chitosan-Induced Stomatal Closure in Arabidopsis thaliana

    Mohammad Issak, Eiji Okuma, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   77 ( 5 )   1111 - 1113   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Salicylic acid (SA), yeast elicitor (YEL), and chitosan (CHT) induced stomatal closure in Arabidopsis wildtype and aba2-2 plants, induced stomatal closure in fluridon-treated wild-type plants, and induced stomatal closure in aos mutants. These results suggest that neither endogenous abscisic acid nor endogenous jasmonic acid is involved in SA-, YEL-, or CHT-induced stomatal closure.

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  • Two guard cell-preferential MAPKs, MPK9 and MPK12, regulate YEL signalling in Arabidopsis guard cells

    M. A. Salam, F. Jammes, M. A. Hossain, W. Ye, Y. Nakamura, I. C. Mori, J. M. Kwak, Y. Murata

    Plant Biology   15 ( 3 )   436 - 442   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We report that two mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate abscisic acid (ABA)-induced stomatal closure in Arabidopsis thaliana. Yeast elicitor (YEL) induced stomatal closure accompanied by intracellular reactive oxygen species (ROS) accumulation and cytosolic free calcium concentration ([Ca2+]cyt) oscillation. In this study, we examined whether these two MAP kinases are involved in YEL-induced stomatal closure using MAPKK inhibitors, PD98059 and U0126, and MAPK mutants, mpk9, mpk12 and mpk9 mpk12. Both PD98059 and U0126 inhibited YEL-induced stomatal closure. YEL induced stomatal closure in the mpk9 and mpk12 mutants but not in the mpk9 mpk12 mutant, suggesting that a MAPK cascade involving MPK9 and MPK12 functions in guard cell YEL signalling. However, YEL induced extracellular ROS production, intracellular ROS accumulation and cytosolic alkalisation in the mpk9, mpk12 and mpk9 mpk12 mutants. YEL induced [Ca2+]cyt oscillations in both wild type and mpk9 mpk12 mutant. These results suggest that MPK9 and MPK12 function redundantly downstream of extracellular ROS production, intracellular ROS accumulation, cytosolic alkalisation and [Ca2+]cyt oscillation in YEL-induced stomatal closure in Arabidopsis guard cells and are shared with ABA signalling. © 2012 German Botanical Society and The Royal Botanical Society of the Netherlands.

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  • Negative Regulation of Methyl Jasmonate-Induced Stomatal Closure by Glutathione in Arabidopsis

    Nasima Akter, Eiji Okuma, Muhammad Abdus Sobahan, Misugi Uraji, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    JOURNAL OF PLANT GROWTH REGULATION   32 ( 1 )   208 - 215   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Glutathione (GSH) has been shown to negatively regulate methyl jasmonate (MeJA)-induced stomatal closure. We investigated the roles of GSH in MeJA signaling in guard cells using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, gamma-glutamylcysteine synthetase. MeJA-induced stomatal closure and decreased GSH contents in guard cells. Decreasing GSH by the cad2-1 mutation enhanced MeJA-induced stomatal closure. Depletion of GSH by the cad2-1 mutation or increment of GSH by GSH monoethyl ester did not affect either MeJA-induced production of reactive oxygen species (ROS) or MeJA-induced cytosolic alkalization in guard cells. MeJA and abscisic acid (ABA) induced stomatal closure and GSH depletion in atrbohD and atrbohF single mutants but not in the atrbohD atrbohF double mutant. Moreover, exogenous hydrogen peroxide induced stomatal closure but did not deplete GSH in guard cells. These results indicate that GSH affects MeJA signaling as well as ABA signaling and that GSH negatively regulates a signal component other than ROS production and cytosolic alkalization in MeJA signal pathway of Arabidopsis guard cells.

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  • Effects of emulsifiers on the photostability of lycopene

    Masayuki Nishino, Makoto Sakata, Yoshiyuki Murata, Yoshimasa Nakamura

    Food Science and Technology Research   19 ( 6 )   983 - 987   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aqueous solutions of dispersed lycopene in which gum arabic, gum ghatti and polyglycerol monostearate ester had been used as emulsifiers were photoirradiated. The photodegradation rate of lycopene tended to vary depending on the amount of polyglycerol monostearate ester used. We found that the photodegradation rate of lycopene with polyglycerol monostearate ester was considerably slower than those with gum arabic and gum ghatti. Therefore, this study suggests that the absorption state of the emulsifier on the crystal surface may affect the photodegradation rate of lycopene.

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  • Arabidopsis thalianaにおけるガンマ線照射誘導過酸化水素蓄積と脂質過酸化の軽減にカタラーゼCAT2は関与しない

    Amena Sultana, 南 育子, 松島 大貴, Mohammad Issak, 中村 宜督, 等々力 節子, 村田 芳行

    食品照射   48 ( 1 )   38 - 41   2013年

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    記述言語:日本語   出版者・発行元:日本食品照射研究協議会  

    シロイヌナズナ野生株(Col-0)及び&lt;i&gt;cat2&lt;/i&gt;変異体への10 kGyのガンマ線照射は,H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;蓄積及び脂質過酸化生成を誘起したが,0.1 kGyと1 kGyのガンマ線照射は,誘起しなかった。また,野生株と変異体との間で,H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;蓄積及び脂質過酸化に有意な差はなかった。以上の結果より,カタラーゼCAT2は,ガンマ線によって誘起される損傷を軽減するための重要な酵素ではないことが示唆された。

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  • Glucosinolate degradation products, isothiocyanates, nitriles, and thiocyanates, induce stomatal closure accompanied by peroxidase-mediated reactive oxygen species production in arabidopsis thaliana

    Mohammad Shakhawat Hossain, Wenxiu Ye, Mohammad Anowar Hossain, Eiji Okuma, Misugi Uraji, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    Bioscience, Biotechnology and Biochemistry   77 ( 5 )   977 - 983   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Isothiocyanates, nitriles, and thiocyanates are degradation products of glucosinolates in crucifer plants. In this study, we investigated the stomatal response to allyl isothiocyanate (AITC), 3-butenenitrile (3BN), and ethyl thiocyanate (ESCN) in Arabidopsis. AITC, 3BN, and ESCN induced stomatal closure in the wild type and the atrbohD atrbohF mutant. Stomatal closure was inhibited by catalase and salicylhydroxamic acid (SHAM). The degradation products induced extracellular reactive oxygen species (ROS) production in the rosette leaves, and intracellular ROS accumulation, NO production, and cytosolic free calcium concentration ([Ca2+]cyt) oscillations in guard cells, which were inhibited by SHAM. These results suggest that glucosinolate degradation products induce stomatal closure accompanied by extracellular ROS production mediated by SHAMsensitive peroxidases, intracellular ROS accumulation, and [Ca 2+]cyt oscillation in Arabidopsis.

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  • Tribolium castaneumの幼虫と成虫へのガンマ線の影響

    Amena Sultana, 南 育子, 市場 隆二, Mohammad Issak, 多田 幹郎, 中村 宜督, 宮竹 貴久, 等々力 節子, 村田 芳行

    食品照射   48 ( 1 )   19 - 23   2013年

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    記述言語:日本語   出版者・発行元:日本食品照射研究協議会  

    ガンマ線を用いた食品照射は,穀物やスパイスの害虫の侵入を抑制する手段の一つである。我々は,&lt;i&gt;Tribolium castaneum&lt;/i&gt;の幼虫と成虫へのガンマ線照射の影響を調べた。羽化は,500Gyの照射で完全に阻害された。成虫の生存率は,500Gy以上の照射で著しく減少した。中性条件でのコメントアッセイの結果より,500Gyまたは1000Gy照射によるDNA損傷は,不可逆的に増加したが,100Gy照射によるDNA損傷は一過的に増加した。100Gy照射ではDNAの修復が行われていることが示唆された。 以上の結果より500Gy以上のガンマ線照射は,&lt;i&gt;T. castaneum &lt;/i&gt;の幼虫と成虫の駆除に十分であることが示された。

    DOI: 10.5986/jrafi.48.19

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  • Effects of Depletion of Glutathione on Abscisic Acid- and Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis thaliana

    Nasima Akter, Muhammad Abdus Sobahan, Misugi Uraji, Wenxiu Ye, Mohammad Anowar Hossain, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   76 ( 11 )   2032 - 2037   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Glutathione (GSH) is involved in abscisic acid (ABA)-and methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we examined the effects of GSH-decreasing chemicals, p-nitrobenzyl chloride (PNBC), iodomethane (IDM), and ethacrynic acid (EA), on ABA- and MeJA-induced stomatal closure in Arabidopsis. Treatments with PNBC, IDM, and EA decreased GSH contents in guard cells. Depletion of GSH by PNBC and IDM enhanced ABA- and MeJA-induced stomatal closure and inhibition of light-induced stomatal opening by ABA, whereas EA did not enhance either ABA- and MeJA-induced stomatal closure or inhibition of light-induced stomatal opening by ABA. Depletion of GSH did not significantly increase the production of the reactive oxygen species (ROS), cytosolic alkalization, or cytosolic Ca2+ oscillation induced by ABA and MeJA. These results indicate that depletion of GSH enhances ABA- and MeJA-induced stomatal closure without affecting ROS production, cytosolic alkalization, or cytosolic Ca2+ oscillation in guard cells of Arabidopsis.

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  • Development and application of a method for identification of isothiocyanate-targeted molecules in colon cancer cells. 国際誌

    Noriyuki Miyoshi, Takumi Yonemochi, Susumu Tomono, Ryuuta Fukutomi, Yoshimasa Nakamura, Hiroshi Ohshima

    Analytical biochemistry   429 ( 2 )   124 - 31   2012年10月

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    記述言語:英語  

    In this study, we have developed a novel method to identify isothiocyanate (ITC)-targeted molecules using two well-studied ITCs: benzyl ITC (BITC) and phenethyl ITC (PEITC). The principle of this method is based on identifying a pattern of differences between BITC and PEITC given that they show similar chemical and biological behaviors. For method validation, dithiothreitol-reduced bovine insulin as a model molecule was incubated with either BITC or PEITC, and digested peptides were analyzed by ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC-TOF-MS) and liquid chromatography quadrupole TOF-MS (LC-Q-TOF-MS). Three peptides-NYCN, FVNQHLCGSHLVE, and ALYLVCGE-were identified as being adducted with BITC or PEITC on their cysteine residues. Each set of peptides adducted with either BITC or PEITC showed retention times (RT(BITC)<RT(PEITC)) by reverse-phase column chromatography with a difference of molecular mass (Δ14.01565). On the basis of these findings, computational mathematical schemes were constructed to extract sets of MS ions satisfying the above criteria. Application of the developed method to an extract of ITC-treated human colon cancer HCT116 cells, thiocarbamoylation of cysteine residues of glutathione, and the N-terminal proline residues of PMFIVNTNVPR from macrophage migration inhibitory factor were successfully identified as one of the intracellular targets of ITCs. Moreover, the method also detected the thiocarbamoylated conjugates of ITCs with intracellular free cysteines and lysines.

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  • Methylglyoxal inhibits seed germination and root elongation and up-regulates transcription of stress-responsive genes in ABA-dependent pathway in Arabidopsis

    T. S. Hoque, M. Uraji, A. Tuya, Y. Nakamura, Y. Murata

    PLANT BIOLOGY   14 ( 5 )   854 - 858   2012年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Methylglyoxal (MG) is a highly reactive metabolite derived from glycolysis. In this study, we examined the effect of MG on seed germination, root elongation, chlorosis and stress-responsive gene expression in Arabidopsis using an abscisic acid (ABA)-deficient mutant, aba2-2. In the wild type, 0.1 mm MG did not affect germination but delayed root elongation, whereas 1.0 mm MG inhibited germination and root elongation and induced chlorosis. MG increased transcription levels of RD29B and RAB18 in a dose-dependent manner but did not affect RD29A transcription level. In contrast, in the aba2-2 mutant, MG inhibition of seed germination at 1.0 mm and 10.0 mm and a delay of root elongation at 0.1 mm MG were mitigated, although there was no significant difference in chlorosis between the wild type and mutant. Moreover, the aba2-2 mutation impaired MG-induced RD29B and RAB18 gene expression. These observations suggest that MG not only directly inhibits germination and root elongation but also indirectly modulates these processes via endogenous ABA in Arabidopsis.

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  • MAP Kinases, MPK9 and MPK12, Regulate Chitosan-Induced Stomatal Closure

    Mohammad Abdus Salam, Fabien Jammes, Mohammad Anowar Hossain, Wenxiu Ye, Yoshimasa Nakamura, Izumi C. Mori, June M. Kwak, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   76 ( 9 )   1785 - 1787   2012年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Chitosan (CHT)-induced stomatal closure was inhibited by an MAPKK inhibitor, PD98059, and was impaired in mpk9 mpk12 but not in mpk9 or mpk12. CHT induced the production of reactive oxygen species, cytosolic alkalization, and cytosolic Ca2+ oscillation in mpk9 mpk12. These results suggest that MPK9 and MPK12 are involved in CHT-induced stomatal closure.

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  • Effects of Exogenous Proline and Glycinebetaine on the Salt Tolerance of Rice Cultivars

    Muhammad Abdus Sobahan, Nasima Akter, Misao Ohno, Eiji Okuma, Yoshihiko Hirai, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   76 ( 8 )   1568 - 1570   2012年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Salinity significantly increased trisodium-8-hydroxy-1,3,6-pyrenetrisulphonic acid (PTS) uptake and decreased the K+/Na+ ratio in salt-sensitive rice (Nipponbare) but did not markedly in salt-tolerant rice (Pokkali). Proline and glycinebetaine (betaine) suppressed the increase in PTS uptake and the decrease in the K+/Na+ ratio in Nipponbare, but did not affect PTS uptake or the K+/Na+ ratio in Pokkali.

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  • Methylglyoxal inhibition of cytosolic ascorbate peroxidase from Nicotiana tabacum

    Md. Anamul Hoque, Misugi Uraji, Akiko Torii, Mst. Nasrin Akhter Banu, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

    JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY   26 ( 8 )   315 - 321   2012年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Methylglyoxal (MG) is one of the aldehydes accumulated in plants under environmental stress. Cytosolic ascorbate peroxidase (cAPX) plays a key role in the protection of cells from oxidative damage by scavenging reactive oxygen species in higher plants. A cDNA encoding cAPX, named NtcAPX, was isolated from Nicotiana tabacum. We characterized recombinant NtcAPX (rNtcAPX) as a fusion protein with glutathione S-transferase to investigate the effects of MG on APX. NtcAPX consists of 250 amino acids and has a deduced molecular mass of 27.5 kDa. The rNtcAPX showed a higher APX activity. MG treatments resulted in a reduction of APX activity and modifications of amino groups in rNtcAPX with increasing Km for ascorbate. On the contrary, neither NaCl nor cadmium reduced the activity of APX. The present study suggests that inhibition of APX is in part due to the modification of amino acids by MG. (c) 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:315321, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21423

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  • Catalases negatively regulate methyl jasrnonate signaling in guard cells

    Rayhanur Jannat, Misugi Uraji, Mohammad Anowar Hossain, Mohammad Muzahidul Islam, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    JOURNAL OF PLANT PHYSIOLOGY   169 ( 10 )   1012 - 1016   2012年7月

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    記述言語:英語   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    Methyl jasmonate (MeJA)-induced stomatal closure is accompanied by the accumulation of hydrogen peroxide (H2O2) in guard cells. In this study, we investigated the roles of catalases (CATs) in MeJA-induced stomatal closure using cat mutants cat2, cat3-1 and cat1 cat3, and the CAT inhibitor, 3-aminotriazole (AT). When assessed with 2',7'-dichlorodihydrofluorescein, the reduction of catalase activity by means of mutations and the inhibitor accumulated higher basal levels of H2O2 in guard cells whereas they did not affect stomatal aperture in the absence of MeJA. In contrast, the cat mutations and the treatment with AT potentiated MeJA-induced stomatal closure and MeJA-induced H2O2 production. These results indicate that CATs negatively regulate H2O2 accumulation in guard cells and suggest that inducible H2O2 production rather than constitutive elevation modulates stomatal apertures in Arabidopsis. (C) 2012 Elsevier GmbH. All rights reserved.

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  • Hydogen peroxide-dependent photocytotoxicity by phloxine B, a xanthene-type food colorant (vol 1810, pg 704, 2011)

    Hang Qi, Hiroshi Takano, Yoji Kato, Qian Wu, Chiharu Ogata, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS   1820 ( 7 )   1020 - 1020   2012年7月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

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  • Methylglyoxal-induced stomatal closure accompanied by peroxidase-mediated ROS production in Arabidopsis

    Tahsina Sharmin Hoque, Misugi Uraji, Wenxiu Ye, Mohammad Anowar Hossain, Yoshimasa Nakamura, Yoshiyuki Murata

    JOURNAL OF PLANT PHYSIOLOGY   169 ( 10 )   979 - 986   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    Methylglyoxal (MG) is an oxygenated short aldehyde and a glycolytic intermediate that accumulates in plants under environmental stresses. Being a reactive alpha-oxoaldehyde, MG may act as a signaling molecule in plants during stresses. We investigated whether MG induces stomatal closure, reactive oxygen species (ROS) production, and cytosolic free calcium concentration ([Ca2+](cyt)) to clarify roles of MG in Arabidopsis guard cells. MG induced production of ROS and [Ca2+](cyt) oscillations, leading to stomatal closure. The MG-induced stomatal closure and ROS production were completely inhibited by a peroxidase inhibitor, salicylhydroxamic acid (SHAM), but were not affected by an NAD(P)H oxidase mutation, atrbohD atrbohF. Furthermore, the MG-elicited [Ca2+](cyt), oscillations were significantly suppressed by SHAM but not by the atrbohD atrbohF mutation. Neither endogenous abscisic acid nor endogenous methyl jasmonate was involved in MG-induced stomatal closure. These results suggest that intrinsic metabolite MG can induce stomatal closure in Arabidopsis accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca2+](cyt) oscillations. (C) 2012 Elsevier GmbH. All rights reserved.

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  • Involvement of intracellular oxidative stress-sensitive pathway in phloxine B-induced photocytotoxicity in human T lymphocytic leukemia cells

    Hang Qi, Beiwei Zhu, Naomi Abe, Yuko Shin, Yoshiyuki Murata, Yoshimasa Nakamura

    FOOD AND CHEMICAL TOXICOLOGY   50 ( 6 )   1841 - 1847   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    We investigated the molecular mechanisms underlying phloxine B (PhB)-induced photocytotoxicity in human T lymphocytic leukemia Jurkat cells. In addition to apoptosis-related biochemical events, photo-irradiated PhB generated intracellular reactive oxygen species (ROS), induced phosphorylation of c-Jun-N-terminal kinase (JNK) in an oxidative stress-dependent manner and up-regulated the gene expression of interferon (IFN)-gamma, an inducer of diverse apoptosis-related molecules in activated T cells. PhB-induced apoptosis was significantly inhibited by N-acetyl-l-cysteine, but not by catalase, indicating that ROS generation occurred intracellularly, and by SP600125 and AG490, specific inhibitors of JNK and IFN-gamma signaling, respectively, confirming their roles in the apoptotic pathway. IFN-gamma up-regulation was also inhibited by SP600125, indicating that it was downstream of INK activation. These results suggest that PhB-induced apoptosis in Jurkat cells partially involves the intracellular oxidative stress-sensitive and T cell-specific IFN-gamma pathway. These data present a novel insight into the mechanisms of photocytotoxicity induced by artificial food colorants in human T lymphocytic leukemia cells. (C) 2012 Elsevier Ltd. All rights reserved.

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  • Mechanisms of the Selenium Tolerance of the Arabidopsis thaliana Knockout Mutant of Sulfate Transporter SULTR1;2

    Misao Ohno, Misugi Uraji, Yasuaki Shimoishi, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   76 ( 5 )   993 - 998   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    We investigated the mechanism of selenium (Se) tolerance using an Arabidopsis thaliana knockout mutant of a sulfate transporter, sultr1;2. Se stress inhibited plant growth, decreased chlorophyll contents, and increased protein oxidation and lipid peroxidation in the wild type, whereas the sultr1;2 mutation mitigated damage of these forms, indicating that sultr1;2 is more tolerant of Se than the wild type is. The accumulation of symplastic Se was suppressed in sultr1;2 as compared to the wild type, and the chemical speciation of Se in the mutant was different from that in the wild type. Regardless of Se stress, the activities of ascorbate peroxidase, catalase, and peroxidase in the mutant were higher than in the wild type, while the activity of superoxide dismutase in the mutant was the same as in the wild type. These results suggest that the sultr1;2 mutation confers Se tolerance on Arabidopsis by decreasing symplastic Se and maintaining antioxidant enzyme activities.

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  • Effect of γ irradiation on the fatty acid composition of soybean and soybean oil

    Ikuko Minami, Yoshimasa Nakamura, Setsuko Todoriki, Yoshiyuki Murata

    Bioscience, Biotechnology and Biochemistry   76 ( 5 )   900 - 905   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Food irradiation is a form of food processing to extend the shelf life and reduce spoilage of food. We examined the effects of γ radiation on the fatty acid composition, lipid peroxidation level, and antioxidative activity of soybean and soybean oil which both contain a large amount of unsaturated fatty acids. Irradiation at 10 to 80 kGy under aerobic conditions did not markedly change the fatty acid composition of soybean. While 10-kGy irradiation did not markedly affect the fatty acid composition of soybean oil under either aerobic or anaerobic conditions, 40-kGy irradiation considerably altered the fatty acid composition of soybean oil under aerobic conditions, but not under anaerobic conditions. Moreover, 40-kGy irradiation produced a significant amount of trans fatty acids under aerobic conditions, but not under anaerobic conditions. Irradiating soybean oil induced lipid peroxidation and reduced the radical scavenging activity under aerobic conditions, but had no effect under anaerobic conditions. These results indicate that the fatty acid composition of soybean was not markedly affected by radiation at 10 kGy, and that anaerobic conditions reduced the degradation of soybean oil that occurred with high doses of γ radiation.

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  • Cooperative function of PLDδ and PLDα1 in abscisic acid-induced stomatal closure in arabidopsis

    Misugi Uraji, Takeshi Katagiri, Eiji Okuma, Wenxiu Ye, Mohammad Anowar Hossain, Choji Masuda, Aya Miura, Yoshimasa Nakamura, Izumi C. Mori, Kazuo Shinozaki, Yoshiyuki Murata

    Plant Physiology   159 ( 1 )   450 - 460   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Phospholipase D (PLD) is involved in responses to abiotic stress and abscisic acid (ABA) signaling. To investigate the roles of two Arabidopsis (Arabidopsis thaliana) PLDs, PLDα1 and PLDδ, in ABA signaling in guard cells, we analyzed ABA responses in guard cells using Arabidopsis wild type, pldα1 and pldδ single mutants, and a pldα1 pldδ double mutant. ABA-induced stomatal closure was suppressed in the pldα1 pldδ double mutant but not in the pld single mutants. The pldα1 and pldδ mutations reduced ABAinduced phosphatidic acid production in epidermal tissues. Expression of either PLDα1 or PLDδ complemented the double mutant stomatal phenotype. ABA-induced stomatal closure in both pldα1 and pldδ single mutants was inhibited by a PLD inhibitor (1-butanol), suggesting that both PLDα1 and PLDδ function in ABA-induced stomatal closure. During ABA-induced stomatal closure, wild-type guard cells accumulate reactive oxygen species and nitric oxide and undergo cytosolic alkalization, but these changes are reduced in guard cells of the pld*alpha;1 pldδ double mutant. Inward-rectifying K + channel currents of guard cells were inhibited by ABA in the wild type but not in the pldα1 pldδ double mutant. ABA inhibited stomatal opening in the wild type and the pldδ mutant but not in the pldα1 mutant. In wild-type rosette leaves, ABA significantly increased PLDδ transcript levels but did not change PLDα1 transcript levels. Furthermore, the pldα1 and pldδ mutations mitigated ABA inhibition of seed germination. These results suggest that PLDα1 and PLDδ cooperate in ABA signaling in guard cells but that their functions do not completely overlap. © 2012 American Society of Plant Biologists. All Rights Reserved.

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  • Luteolin Overcomes Resistance to Benzyl Isothiocyanate-Induced Apoptosis in Human Colorectal Cancer HCT-116 Cells

    Rieko Sakai, Shintaro Yokobe, Naomi Abe, Noriyuki Miyoshi, Yoshiyuki Murata, Yoshimasa Nakamura

    JOURNAL OF FOOD AND DRUG ANALYSIS   20   389 - 393   2012年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FOOD & DRUG ADMINSTRATION  

    We have previously identified p53, a universal sensor of genotoxic stress, as a negative regulator of the apoptosis induction by benzyl isothiocyanate (BITC) in the normal colon fibroblastoid cells. In the present study, we further confirmed that BITC has a potential to induce cytotoxicity in the p53-mutated colon cancer HT-29 cells in preference to HCT-116 cells with wild-type p53. To obtain effective induction of BITC-stimulated apoptosis in p53-positive cells, we investigated the combination effect of BITC and food ingredient that may overcome resistance to BITC. Pretreatment with luteolin potentiated the cytotoxicity induction by BITC in HCT-116 cells but not in HT-29 cells. The biochemical events related to apoptosis such as DNA ladder formation and caspase-3 activation were also enhanced by luteolin. Luteolin attenuated the expression of p21(wafl/cipl) a key downstream target of p53. These results suggest the role of p21(wafl/cipl) pathway in the overcoming BITC resistance by luteolin.

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  • Increased halogenated tyrosine levels are useful markers of human skin ageing, reflecting proteins denatured by past skin inflammation

    Y. Ishitsuka, F. Maniwa, C. Koide, Y. Kato, Y. Nakamura, T. Osawa, M. Tanioka, Y. Miyachi

    CLINICAL AND EXPERIMENTAL DERMATOLOGY   37 ( 3 )   252 - 258   2012年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Background. Photoageing of skin is thought to be caused by protein denaturation, which can be induced by ultraviolet radiation. Previous studies have also reported that inflammation is related to protein denaturation; however, the influence of inflammation on skin ageing has not been explored in detail.
    Aim. To investigate the possible connection between inflammation and protein denaturation, which might lead to skin ageing, we focused on halogenated tyrosine as a denatured substance produced during the inflammation process.
    Methods. We measured halogenated tyrosine in aged human skin. Inflammatory cells and halogenated tyrosine were detected by immunohistochemistry using antibodies to mast-cell tryptase, neutrophilic myeloperoxidase and halogenated tyrosine. Finally, using elastic van Gieson ( EVG) staining, we investigated whether the sites of halogenated tyrosine coincided with the sites at which proteins were denatured.
    Results. Immunohistochemical analysis indicated that both inflammatory cells and halogenated tyrosines increased with ageing in both photoexposed and photoprotected skin. EVG staining confirmed that the localization of halogenated tyrosine was close to the sites at which protein was denatured.
    Conclusions. Our investigations indicate a possible connection between skin ageing and inflammation, suggesting that halogenated tyrosine could be a useful marker of ageing skin.

    DOI: 10.1111/j.1365-2230.2011.04215.x

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  • Inhibitory Effects of Methylglyoxal on Light-Induced Stomatal Opening and Inward K+ Channel Activity in Arabidopsis

    Tahsina Sharmin Hoque, Eiji Okuma, Misugi Uraji, Takuya Furuichi, Takayuki Sasaki, Md Anamul Hoque, Yoshimasa Nakamura, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   76 ( 3 )   617 - 619   2012年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Methylglyoxal (MG) is a reactive aldehyde derived by glycolysis. In Arabidopsis, MG inhibited light-induced stomatal opening in a dose-dependent manner. It significantly inhibited both inward-rectifying potassium (K-in) channels in guard-cell protoplasts and an Arabidopsis K-in channel, KAT1, heterologously expressed in Xenopus oocytes. Thus it appears that MG inhibition of stomatal opening involves MG inhibition of K+ influx into guard cells.

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  • FIA functions as an early signal component of abscisic acid signal cascade in Vicia faba guard cells

    Yusuke Sugiyama, Misugi Uraji, Megumi Watanabe-Sugimoto, Eiji Okuma, Shintaro Munemasa, Yasuaki Shimoishi, Yoshimasa Nakamura, Izumi C. Mori, Sumio Iwai, Yoshiyuki Murata

    JOURNAL OF EXPERIMENTAL BOTANY   63 ( 3 )   1357 - 1365   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    An abscisic acid (ABA)-insensitive Vicia faba mutant, fia (fava bean impaired in ABA-induced stomatal closure) had previously been isolated. In this study, it was investigated how FIA functions in ABA signalling in guard cells of Vicia faba. Unlike ABA, methyl jasmonate (MeJA), H2O2, and nitric oxide (NO) induced stomatal closure in the fia mutant. ABA did not induce production of either reactive oxygen species or NO in the mutant. Moreover, ABA did not suppress inward-rectifying K+ (K-in) currents or activate ABA-activated protein kinase (AAPK) in mutant guard cells. These results suggest that FIA functions as an early signal component upstream of AAPK activation in ABA signalling but does not function in MeJA signalling in guard cells of Vicia faba.

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  • α-tocopherol sensitizes human leukemia HL-60 cells to apoptosis induced by benzyl isothiocyanate

    Naomi Abe, Tomomi Shimizu, Noriyuki Miyoshi, Yoshiyuki Murata, Yoshimasa Nakamura

    Bioscience, Biotechnology and Biochemistry   76 ( 2 )   381 - 383   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Pre-treatment with α-tocopherol (α-Toc) potentiated cytotoxicity induction by benzyl isothiocyanate (BITC). Biochemical events related to apoptosis, such as DNA ladder formation and caspase-3 activation, were also enhanced by α-Toc. These results suggest a significant role of the caspase-3 pathway in apoptosis induction regulated by α-Toc in combination. © 2012 W. S. Maney &amp; Son Ltd.

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  • Cytotoxicity of Benzyl Isothiocyanate in Normal Renal Proximal Tubular Cells and Its Modulation by Glutathione

    Naomi Abe, Masashi Okuhira, Chiharu Tsutsui, Yoshiyuki Murata, Yoshimasa Nakamura

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   60 ( 7 )   1887 - 1892   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    In the present study, we examined the toxicity of benzyl 1TC (BITC) and its urinary mercapturic acid metabolite (BITC-NAC), using a normal renal proximal tubular cell line, pig LLC-PK1. BITC increased cell death with an IC50 value of about 7 mu M, whereas the cytotoxic effect of BITC-NAC was five times weaker than that of BITC. We observed a significant necrosis of the compounds on LLC-PK1 cells with oxidative stress. In the presence of 5 mM glutathione (GSH), comparable to physiological levels, the cytotoxicity of BITC-NAC as well as BITC was significantly reduced. Furthermore, the increase in intracellular GSH levels by pretreatment with NAC before the BITC treatment resulted ill inhibition of the BITC-induced necrotic events as well as intracellular oxidative stress. These results suggest that GSH is a determinant of cellular resistance against the BITC-mediated and oxidative stress-dependent cytotoxicity in renal proximal tubular cells.

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  • K252a-sensitive protein kinases but not okadaic acid-sensitive protein phosphatases regulate methyl jasmonate-induced cytosolic Ca2+ oscillation in guard cells of Arabidopsis thaliana

    Mohammad Anowar Hossain, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    JOURNAL OF PLANT PHYSIOLOGY   168 ( 16 )   1901 - 1908   2011年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    Methyl jasmonate (MeJA) induces stomatal closure similar to abscisic acid (ABA), and MeJA signaling in guard cells shares some signal components with ABA signaling. As part of this process, MeJA as well as ABA induce the elevation and oscillation of cytosolic free-calcium concentrations ([Ca2+](cyt)) in guard cells. While abscisic acid-induced [Ca2+](cyt) oscillation has been extensively studied, MeJA-induced [Ca2+](cyt) oscillation is less well understood. In this study, we investigated the effects of K252a (a broad-range protein kinase inhibitor) and okadaic acid (OA, a protein phosphatase 1 and 2A inhibitor) on MeJA-induced [Ca2+](cyt) oscillation in guard cells of Arabidopsis thaliana ecotype Columbia expressing the Ca2+ reporter yellow cameleon 3.6. The protein kinase inhibitor K252a abolished MeJA-induced stomatal closure and reduced MeJA-elicited [Ca2+](cyt) oscillation. The protein phosphatase inhibitor OA, on the other hand, did not inhibit these processes. These results suggest that MeJA signaling involves activation of K252a-sensitive protein kinases upstream of [Ca2+](cyt) oscillation but not activation of an OA-sensitive protein phosphatase in guard cells of A. thaliana ecotype Columbia. (C) 2011 Elsevier GmbH. All rights reserved.

    DOI: 10.1016/j.jplph.2011.05.004

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  • Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis

    Md. Atiqur Rahman Khokon, Md. Sarwar Jahan, Taniya Rahman, Mohammad Anowar Hossain, Daichi Muroyama, Ikuko Minami, Shintaro Munemasa, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

    PLANT CELL AND ENVIRONMENT   34 ( 11 )   1900 - 1906   2011年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure in Arabidopsis via production of reactive oxygen species (ROS) and nitric oxide (NO), and elevation of cytosolic Ca2+. AITC-induced stomatal closures were partially inhibited by an inhibitor of NADPH oxidase and completely inhibited by glutathione monoethyl ester (GSHmee). AITC-induced stomatal closure and ROS production were examined in abscisic acid (ABA) deficient mutant aba2-2 and methyl jasmonate (MeJA)-deficient mutant aos to elucidate involvement of endogenous ABA and MeJA. Genetic evidences have demonstrated that AITC-induced stomatal closure required MeJA priming but not ABA priming. These results raise the possibility that crucifer plants produce ITCs to induce stomatal closure, leading to suppression of water loss and invasion of fungi through stomata.

    DOI: 10.1111/j.1365-3040.2011.02385.x

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  • Roles of intracellular hydrogen peroxide accumulation in abscisic acid signaling in Arabidopsis guard cells

    Rayhanur Jannat, Misugi Uraji, Miho Morofuji, Mohammad Muzahidul Islam, Rachel E. Bloom, Yoshimasa Nakamura, C. Robertson McClung, Julian I. Schroeder, Izumi C. Mori, Yoshiyuki Murata

    JOURNAL OF PLANT PHYSIOLOGY   168 ( 16 )   1919 - 1926   2011年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    Reactive oxygen species (ROS), including hydrogen peroxide (H(2)O(2)), are among the important second messengers in abscisic acid (ABA) signaling in guard cells. In this study, to investigate specific roles of H(2)O(2) in ABA signaling in guard cells, we examined the effects of mutations in the guard cell-expressed catalase (CAT) genes, CAT1 and CAT3, and of the CAT inhibitor 3-aminotriazole (AT) on stomata! movement. The cat3 and cat1 cat3 mutations significantly reduced CAT activities, leading to higher basal level of H(2)O(2) in guard cells, when assessed by 2&apos;,7&apos;-dichlorodihydrofluorescein, whereas they did not affect stomatal aperture size under non-stressed condition. In addition, AT-treatment at concentrations that abolish CAT activities, showed trivial affect on stomatal aperture size, while basal H(2)O(2) level increased extensively. In contrast, cat mutations and AT-treatment potentiated ABA-induced stomata! closure. Inducible ROS production triggered by ABA was observed in these mutants and wild type as well as in AT-treated guard cells. These results suggest that ABA-inducible cytosolic H(2)O(2) elevation functions in ABA-induced stomatal closure, while constitutive increase of H(2)O(2) do not cause stomatal closure. (C) 2011 Elsevier GmbH. All rights reserved.

    DOI: 10.1016/j.jplph.2011.05.006

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  • Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis

    Eiji Okuma, Md Sarwar Jahan, Shintaro Munemasa, Mohammad Anowar Hossain, Daichi Muroyama, Mohammad Mahbub Islam, Ken'ichi Ogawa, Megumi Watanabe-Sugimoto, Yoshimasa Nakamura, Yasuaki Shimoishi, Izumi C. Mori, Yoshiyuki Murata

    JOURNAL OF PLANT PHYSIOLOGY   168 ( 17 )   2048 - 2055   2011年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant. cad2-1. that is deficient in the first GSH biosynthesis enzyme, gamma-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomata! closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomata! closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca2+ oscillation in ABA signal pathway of Arabidopsis guard cells. (C) 2011 Elsevier GmbH. All rights reserved.

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  • Localized cysteine sulfenic acid formation by vascular endothelial growth factor: role in endothelial cell migration and angiogenesis

    Nihal Kaplan, Norifumi Urao, Eiji Furuta, Seok-Jo Kim, Masooma Razvi, Yoshimasa Nakamura, Ronald D. McKinney, Leslie B. Poole, Tohru Fukai, Masuko Ushio-Fukai

    FREE RADICAL RESEARCH   45 ( 10 )   1124 - 1135   2011年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INFORMA HEALTHCARE  

    Reactive oxygen species (ROS) are important mediators for VEGF receptor 2 (VEGFR2) signalling involved in angiogenesis. The initial product of Cys oxidation, cysteine sulfenic acid (Cys-OH), is a key intermediate in redox signal transduction; however, its role in VEGF signalling is unknown. We have previously demonstrated IQGAP1 as a VEGFR2 binding scaffold protein involved in ROS-dependent EC migration and post-ischemic angiogenesis. Using a biotin-labelled Cys-OH trapping reagent, we show that VEGF increases protein-Cys-OH formation at the lamellipodial leading edge where it co-localizes with NADPH oxidase and IQGAP1 in migrating ECs, which is prevented by IQGAP1 siRNA or trapping of Cys-OH with dimedone. VEGF increases IQGAP1-Cys-OH formation, which is prevented by N-acetyl cysteine or dimedone, which inhibits VEGF-induced EC migration and capillary network formation. In vivo, hindlimb ischemia in mice increases CysOH formation in small vessels and IQGAP1 in ischemic tissues. In summary, VEGF stimulates localized formation of Cys-OH-IQGAP1 at the leading edge, thereby promoting directional EC migration, which may contribute to post-natal angiogenesis in vivo. Thus, targeting Cys-oxidized proteins at specific compartments may be the potential therapeutic strategy for various angiogenesis-dependent diseases.

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  • The Roles of CATALASE2 in Abscisic Acid Signaling in Arabidopsis Guard Cells

    Rayhanur Jannat, Misugi Uraji, Miho Morofuji, Mohammad Anowar Hossain, Mohammad Muzahidul Islam, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   75 ( 10 )   2034 - 2036   2011年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    We investigated the roles of catalase (CAT) in abscisic acid (ABA)-induced stomatal closure using a cat2 mutant and an inhibitor of CAT, 3-aminotriazole (AT). Constitutive reactive oxygen species (ROS) accumulation due to the CAT2 mutation and AT treatment did not affect stomatal aperture in the absence of ABA, whereas ABA-induced stomatal closure, ROS production, and [Ca2+](cyt) oscillation were enhanced.

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  • Histochemical quantification of glutathione contents in guard cells of Arabidopsis thaliana

    Md. Sarwar Jahan, Yoshimasa Nakamura, Yoshiyuki Murata

    SCIENCEASIA   37 ( 3 )   281 - 284   2011年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:THAILANDS NATL SCIENCE & TECHNOLOGY DEVELOPMENT AGENCY  

    Glutathione (GSH), one of the most abundant low molecular-weight thiol compounds, maintains redox homoeostasis in plants. To measure GSH content in guard cells of Arabidopsis thaliana, we have developed a technique to quantify the GSH content in guard cells using the staining dye monochlorobimane (MCB). The fluorescent intensity of glutathione S-bimane (GSB) gradually increased in guard cells with increasing incubation time and reached steady state 2 h after epidermal tissues were treated with MCB. The guard cells contained larger amount of GSH content than other cells of the leaves except trichome cell. MCB staining showed that guard cells of a GSH deficient mutant, accumulated significantly lower GSH content than that of wild type guard cells. When the guard cells were treated with 1-chloro-2,4-dinitrobenzene to reduce GSH content in the guard cells, the GSB fluorescence in the guard cells was abolished. In addition, GSH monoethyl ester restored GSH level in the guard cells of ch1-1 mutant plants. These results suggest that GSH content in guard cells can be quantified using MCB dye.

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  • Hydogen peroxide-dependent photocytotoxicity by phloxine B, a xanthene-type food colorant

    Hang Qi, Hiroshi Takano, Yoji Kato, Qian Wu, Chiharu Ogata, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS   1810 ( 7 )   704 - 712   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Background: Phloxine B (PhB; 2&apos;,4&apos;,5&apos;,7&apos;-tetrabromo-4,5,6,7-tetrachloro-fluorescein), an artificial xanthene colorant, has been used as a red coloring agent in drugs and cosmetics as well as foods in some countries. However, little effort has been devoted to the study of this colorant as a potentially useful medicinal agent.
    Methods: We investigated the daily light-induced photocytotoxicity of PhB in two human leukemia cells, HL-60 and Jurkat, and its underlying mechanisms by in vitro experiments using antioxidants.
    Reuslts and conclusions: PhB inhibited cell proliferation more preferentially to HL-60 cells than to Jurkat cells. Co-treatment of catalase completely blocked the photocytotoxicity by PhB in HL-60 cells, whereas the effect of histidine was only partial, suggesting that hydrogen peroxide (H(2)O(2)), rather than singlet oxygen, might be a prerequisite for the PhB-induced HL-60 cell death. Actually, PhB produced a significant amount of H(2)O(2) in the media as well as in the cells in concentration- and light-dependent manners. Furthermore, methionine, a hypochlorous acid (HOCl) scavenger, also significantly attenuated the cytotoxicity in HL-60 cells, but not in Jurkat cells, indicating the involvement of myeloperoxidase (MPO)-dependent hypohalous acid formation during the photocytotoxicity. In vitro experiments revealed that halogenated tyrosine was generated from the reaction of bovine serum albumin with PhB and HL-60 cell lysate. The present findings suggested that PhB induced a differential photodynamic action in the MPO-containing leukemia cells through an H(2)O(2)-dependent mechanism.
    General significance: Our findings provide new insights into the molecular mechanisms underlying the PhB-induced apoptosis and also evaluated PhB as a promising PDT agent. (C) 2011 Elsevier B.V. All rights reserved.

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  • Photostability of Lycopene Dispersed in an Aqueous Solution

    Masayuki Nishino, Takeshi Miuchi, Makoto Sakata, Atsushi Nishida, Yoshiyuki Murata, Yoshimasa Nakamura

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   75 ( 7 )   1389 - 1391   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Lycopene dispersed in aqueous solutions with different dissolved oxygen contents was photo-irradiated by using a xenon weather meter, and the contents of lycopene and dissolved oxygen were measured. Both the degradation of lycopene and the consumption of dissolved oxygen followed a first-order kinetics model. There was a proportional relationship between the degradation content of lycopene and the consumption of dissolved oxygen. These results indicate that dissolved oxygen would also be involved in the photolysis of lycopene.

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  • Involvement of Endogenous Abscisic Acid in Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis

    Mohammad Anowar Hossain, Shintaro Munemasa, Misugi Uraji, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    PLANT PHYSIOLOGY   156 ( 1 )   430 - 438   2011年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    In this study, we examined the involvement of endogenous abscisic acid (ABA) in methyl jasmonate (MeJA)-induced stomatal closure using an inhibitor of ABA biosynthesis, fluridon (FLU), and an ABA-deficient Arabidopsis (Arabidopsis thaliana) mutant, aba2-2. We found that pretreatment with FLU inhibited MeJA-induced stomatal closure but not ABA-induced stomatal closure in wild-type plants. The aba2-2 mutation impaired MeJA-induced stomatal closure but not ABA-induced stomatal closure. We also investigated the effects of FLU and the aba2-2 mutation on cytosolic free calcium concentration ([Ca2+](cyt)) in guard cells using a Ca2+-reporter fluorescent protein, Yellow Cameleon 3.6. In wild-type guard cells, FLU inhibited MeJA-induced [Ca2+](cyt) elevation but not ABA-induced [Ca2+](cyt) elevation. The aba2-2 mutation did not affect ABA-elicited [Ca2+](cyt) elevation but suppressed MeJA-induced [Ca2+](cyt) elevation. We also tested the effects of the aba2-2 mutation and FLU on the expression of MeJA-inducible VEGETATIVE STORAGE PROTEIN1 (VSP1). In the aba2-2 mutant, MeJA did not induce VSP1 expression. In wild-type leaves, FLU inhibited MeJA-induced VSP1 expression. Pretreatment with ABA at 0.1 mu M, which is not enough concentration to evoke ABA responses in the wild type, rescued the observed phenotypes of the aba2-2 mutant. Finally, we found that in wild-type leaves, MeJA stimulates the expression of 9-CIS-EPOXYCAROTENOID DIOXYGENASE3, which encodes a crucial enzyme in ABA biosynthesis. These results suggest that endogenous ABA could be involved in MeJA signal transduction and lead to stomatal closure in Arabidopsis guard cells.

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  • (-)-Epigallocatechin-3-gallate suppresses growth of AZ521 human gastric cancer cells by targeting the DEAD-box RNA helicase p68

    Tomoko Tanaka, Takeshi Ishii, Daisuke Mizuno, Taiki Mori, Ryoichi Yamaji, Yoshimasa Nakamura, Shigenori Kumazawa, Tsutomu Nakayama, Mitsugu Akagawa

    FREE RADICAL BIOLOGY AND MEDICINE   50 ( 10 )   1324 - 1335   2011年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    (-)-Epigallocatechin-3-gallate (EGCG), the most abundant and biologically active polyphenol in green tea, induces apoptosis and suppresses proliferation of cancer cells by modulating multiple signal transduction pathways. However, the fundamental mechanisms responsible for these cancer-preventive effects have not been clearly elucidated. Recently, we found that EGCG can covalently bind to cysteine residues in proteins through autoxidation and subsequently modulate protein function. In this study, we demonstrate the direct binding of EGCG to cellular proteins in AZ521 human gastric cancer cells by redox-cycle staining. We comprehensively explored the binding targets of EGCG from EGCG-treated A2521 cells by proteomics techniques combined with the boronate-affinity pull-down method. The DEAD-box RNA helicase p68, which is overexpressed in a variety of tumor cells and plays an important role in cancer development and progression, was identified as a novel EGCG-binding target. Exposure of AZ521 cells to EGCG lowered the p68 level dose dependently. The present findings show that EGCG inhibits AZ521 cell proliferation by preventing beta-catenin oncogenic signaling through proteasomal degradation of p68 and provide a new perspective on the molecular mechanism of EGCG action. (C) 2011 Elsevier Inc. All rights reserved.

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  • Involvement of extracellular oxidative burst in salicylic acid-induced stomatal closure in Arabidopsis

    Md. Atiqur Rahman Khokon, Eiji Okuma, Mohammad Anowar Hossain, Shintaro Munemasa, Misugi Uraji, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    PLANT CELL AND ENVIRONMENT   34 ( 3 )   434 - 443   2011年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Salicylic acid (SA), a ubiquitous phenolic phytohormone, is involved in many plant physiological processes including stomatal movement. We analysed SA-induced stomatal closure, production of reactive oxygen species (ROS) and nitric oxide (NO), cytosolic calcium ion ([Ca2+](cyt)) oscillations and inward-rectifying potassium (K+(in)) channel activity in Arabidopsis. SA-induced stomatal closure was inhibited by pre-treatment with catalase (CAT) and superoxide dismutase (SOD), suggesting the involvement of extracellular ROS. A peroxidase inhibitor, SHAM (salicylhydroxamic acid) completely abolished SA-induced stomatal closure whereas neither an inhibitor of NADPH oxidase (DPI) nor atrbohD atrbohF mutation impairs SA-induced stomatal closures. 3,3&apos;-Diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) stainings demonstrated that SA induced H(2)O(2) and O(2)- production. Guard cell ROS accumulation was significantly increased by SA, but that ROS was suppressed by exogenous CAT, SOD and SHAM. NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) suppressed the SA-induced stomatal closure but did not suppress guard cell ROS accumulation whereas SHAM suppressed SA-induced NO production. SA failed to induce [Ca2+](cyt) oscillations in guard cells whereas K+(in) channel activity was suppressed by SA. These results indicate that SA induces stomatal closure accompanied with extracellular ROS production mediated by SHAM-sensitive peroxidase, intracellular ROS accumulation and K+(in) channel inactivation.

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  • The Arabidopsis Calcium-Dependent Protein Kinase, CPK6, Functions as a Positive Regulator of Methyl Jasmonate Signaling in Guard Cells

    Shintaro Munemasa, Mohammad Anowar Hossain, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    PLANT PHYSIOLOGY   155 ( 1 )   553 - 561   2011年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Previous studies have demonstrated that methyl jasmonate (MeJA) induces stomatal closure dependent on change of cytosolic free calcium concentration in guard cells. However, these molecular mechanisms of intracellular Ca2+ signal perception remain unknown. Calcium-dependent protein kinases (CDPKs) function as Ca2+ signal transducers in various plant physiological processes. It has been reported that four Arabidopsis (Arabidopsis thaliana) CDPKs, CPK3, CPK6, CPK4, and CPK11, are involved in abscisic acid signaling in guard cells. It is also known that there is an interaction between MeJA and abscisic acid signaling in guard cells. In this study, we examined the roles of these CDPKs in MeJA signaling in guard cells using Arabidopsis mutants disrupted in the CDPK genes. Disruption of the CPK6 gene impaired MeJA-induced stomatal closure, but disruption of the other CDPK genes did not. Despite the broad expression pattern of CPK6, we did not find other remarkable MeJA-insensitive phenotypes in the cpk6-1 mutant. The whole-cell patch-clamp analysis revealed that MeJA activation of nonselective Ca2+-permeable cation channels is impaired in the cpk6-1 mutant. Consistent with this result, MeJA-induced transient cytosolic free calcium concentration increments were reduced in the cpk6-1 mutant. MeJA failed to activate slow-type anion channels in the cpk6-1 guard cells. Production of early signal components, reactive oxygen species and nitric oxide, in guard cells was elicited by MeJA in the cpk6-1 mutant as in the wild type. These results provide genetic evidence that CPK6 has a different role from CPK3 and functions as a positive regulator of MeJA signaling in Arabidopsis guard cells.

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  • The Involvement of Intracellular Glutathione in Methyl Jasmonate Signaling in Arabidopsis Guard Cells

    Nasima Akter, Muhammad Abdus Sobahan, Mohammad Anowar Hossain, Misugi Uraji, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74 ( 12 )   2504 - 2506   2010年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    We examined the involvement of intracellular glutathione (GSH) in methyl jasmonate (MeJA) signaling. The chlorinal-1 (ch1-1) mutation decreased GSH in guard cells and narrowed the stomata! aperture. GSH monoethyl ester increased intracellular GSH, diminishing this phenotype. GSH did not affect MeJA-induced reactive oxygen species production or cytosolic Ca2+ oscillation, suggesting that GSH modulates MeJA signaling downstream of production and oscillation.

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  • Covalent Binding of Tea Catechins to Protein Thiols: The Relationship between Stability and Electrophilic Reactivity

    Taiki Mori, Takeshi Ishii, Mitsugu Akagawa, Yoshimasa Nakamura, Tsutomu Nakayama

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74 ( 12 )   2451 - 2456   2010年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    In this study, we investigated the relationship between the stability of catechins and their electrophilic reactivity with proteins. The stability of catechins was evaluated by HPLC analysis. Catechol-type catechins were stable in a neutral buffer, but pyrogallol-type catechins, such as (-)-epigallocatechin gallate (EGCg), were unstable. The electrophilic reactivity of catechins with thiol groups in a model peptide and a protein was confirmed by both mass spectrometry and electrophoresis/blotting with redox-cycling staining. In a comparison of several catechins, pyrogallol-type catechins had higher reactivity with protein thiols than catechol-type catechins. The instability and reactivity of EGCg were enhanced in an alkaline pH buffer. The reactivity of EGCg was reduced by antioxidants due to their ability to prevent EGCg autoxidation. These results indicate that the instability against oxidation of catechins is profoundly related to their electrophilic reactivity. Consequently, the difference in these properties of tea catechins can contribute to the magnitude of their biological activities.

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  • Yeast Elicitor-Induced Stomatal Closure and Peroxidase-Mediated ROS Production in Arabidopsis

    Md. Atiqur Rahman Khokon, Mohammad Anowar Hossain, Shintaro Munemasa, Misugi Uraji, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    PLANT AND CELL PHYSIOLOGY   51 ( 11 )   1915 - 1921   2010年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Yeast elicitor (YEL) induces stomatal closure. We investigated reactive oxygen species (ROS) production, nitric oxide (NO) production and [Ca(2+)](cyt) oscillations to clarify YEL signaling in Arabidopsis guard cells. YEL induced ROS accumulation in guard cells. A peroxidase inhibitor [salicylhydroxamic acid (SHAM)] inhibited the stomatal closure and the ROS accumulation, but neither the atrbohD atrbohF mutation nor an NADPH oxidase inhibitor [diphenylene iodonium chloride (DPI)] had any effect. An NO scavenger [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3oxide (cPTIO)] inhibited the YEL-induced stomatal closure and SHAM abolished NO production. YEL-elicited [Ca(2+)] cyt oscillations were inhibited by SHAM but not by the atrbohD atrbohF mutation. These results indicate that YEL induces stomatal closure accompanied by ROS production mediated by peroxidases and NO production.

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  • Chitosan-Induced Stomatal Closure Accompanied by Peroxidase-Mediated Reactive Oxygen Species Production in Arabidopsis

    Md Atiqur Rahman Khokon, Misugi Uraji, Shintaro Munemasa, Eiji Okuma, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74 ( 11 )   2313 - 2315   2010年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Chitosan induced stomatal closure in wild type-plants and NADPH oxidase knock-out mutants (atrbohD atrbohF), and reactive oxygen species (ROS) production in wild-type guard cells. Closure and production were completely abolished by catalase and a peroxidase inhibitor. These results indicate that chitosan induces ROS production mediated by peroxidase, resulting in stomata! closure.

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  • The Effects of Methylglyoxal on Glutathione S-Transferase from Nicotiana tabacum

    Md Anamul Hoque, Misugi Uraji, Mst Nasrin Akhter Banu, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74 ( 10 )   2124 - 2126   2010年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Methylglyoxal (MG) is one of the aldehydes that accumulate in plants under environmental stress. Glutathione S-transferases (GSTs) play important roles, including detoxification, in the stress tolerance systems of plants. To determine the effects of MG, we characterized recombinant GST. MG decreased GST activity and thiol contents with increasing Km. GST can serve as a target of MG modification, which is suppressed by application of reduced glutathione.

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  • Cytosolic Alkalization and Cytosolic Calcium Oscillation in Arabidopsis Guard Cells Response to ABA and MeJA

    Mohammad Mahbub Islam, Mohammad Anowar Hossain, Rayhanur Jannat, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    PLANT AND CELL PHYSIOLOGY   51 ( 10 )   1721 - 1730   2010年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure are accompanied by cytosolic alkalization in guard cells. However, it remains to be clarified how the alkalization functions in not only ABA signaling but also MeJA. We investigated cytosolic alkalization in guard cells during ABA-, MeJA- and Ca(2+)-induced stomatal closure of wild type, abi1-1, abi2-1, ost1-2 and coi1 using a pH-sensitive fluorescent dye, BCECF-AM. ABA induced cytosolic alkalization in guard cells of wild-type and coi1 but not in ost1-2 guard cells whereas MeJA elicited cytosolic alkalization in wild-type and ost1-2 guard cells but not in coi1. Neither ABA nor MeJA induced cytosolic alkalization in abi1-1 and abi2-1 guard cells. Exogenous Ca(2+) induced stomatal closure accompanied by cytosolic alkalization in guard cells of wild-type, abi1-1, abi2-1, ost1-2 and coi1 plants. An agent to acidify cytosol, butyrate, suppressed Ca(2+) -induced cytosolic alkalization and ABA-, MeJA- and Ca(2+) -induced cytosolic Ca(2+) oscillation in wild-type guard cells to prevent stomatal closure. These results indicate that cytosolic alkalization and cytosolic Ca(2+) oscillation coordinately function in ABA and MeJA signaling in Arabidopsis guard cells.

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  • Proline and Glycinebetaine Ameliorated NaCl Stress via Scavenging of Hydrogen Peroxide and Methylglyoxal but Not Superoxide or Nitric Oxide in Tobacco Cultured Cells

    Mst Nasrin Akhter Banu, Md Anamul Hoque, Megumi Watanabe-Sugimoto, Mohammad Muzahidul Islam, Misugi Uraji, Ken Matsuoka, Yoshimasa Nakamura, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74 ( 10 )   2043 - 2049   2010年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Efficient detoxification of the reactive oxygen species, nitric oxide (NO) and methylglyoxal (MG), provides protection against NaCl-induced damage in plants. To elucidate the protective mechanisms of proline and glycinebetaine (betaine) against NaCl stress, intracellular levels of hydrogen peroxide (H2O2), superoxide (O-2(-)), NO, and MG were investigated in tobacco Bright Yellow-2 cells. The Levels of H2O2, O-2(-), NO and MG were higher in the short-term and long-term NaCl-stressed cells than in the non-stressed cells, whereas the O-2(-) level was higher in the long-term stressed cells. Exogenous proline and betaine decreased the H2O2 level in both the short-term and the long-term NaCl-stressed cells and the MG level in the long-term NaCl-stressed cells, but did not change the O-2(-) or NO levels. Under salt stress, both proline and betaine increased the transcription levels of glutathione peroxidase, which can contribute to the reduction of H2O2. In conclusion, proline and betaine mitigated salt stress via reduction of H2O2 accumulation during short-term incubation and via reduction of the accumulation of H2O2 and MG during long-term incubation.

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  • Artificial Food Colorants Inhibit Superoxide Production in Differentiated HL-60 Cells

    Hang Qi, Machiko Kawagishi, Motoko Yoshimoto, Hiroshi Takano, Beiwei Zhu, Yasuaki Shimoishi, Mikiro Tada, Yoshiyuki Murata, Yoshimasa Nakamura

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74 ( 8 )   1725 - 1728   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    We tested synthetic food colorants for their antioxidative potential by the in vitro superoxide generation assay in differentiated HL-60 cells in response to phorbol ester. Among the 12 colorants tested, such fluorescein-type red colorants as rose bengal showed potent inhibitory activity without any cytotoxicity under dark conditions. The intracellular accumulation and superoxide anion scavenging effect of rose bengal were at least partly involved in the inhibitory activity.

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  • Electrophiles in Foods: The Current Status of Isothiocyanates and Their Chemical Biology

    Yoshimasa Nakamura, Noriyuki Miyoshi

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74 ( 2 )   242 - 255   2010年2月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    Unlike many classical signals and hormones, exposure of the cells to electrophilic molecules potentially induces a series of characteristic and wide-ranging biological responses by covalently attaching with macromolecules such as proteins as well as small cellular reductants. In addition to chemicals originated from xenobiotics or lipid peroxidation, electrophiles in foods have recently attracted much attention. These compounds have recently been found to induce expression of cytoprotective proteins that are involved in the elimination or inactivation of oxidative stress and carcinogenic electrophiles implicated in several pathogeneses. The redox-sensitive regulating systems such as Keap1/Nrf2/ARE play a key role in this induction and thus are considered to be the most important target of electrophiles in foods. This review highlights the food-derived electrophiles as promising protectors against various diseases, with an emphasis on possible molecular mechanisms. Current knowledge of isothiocyanates (ITCs), representative electrophile compounds from cruciferous vegetables, is discussed also, with consideration of the chemistry, metabolism, absorption, and factors influencing the biological activities of ITCs. In addition, this review attempts to provide a balanced perspective on the relative beneficial and harmful effects of the food electrophiles.

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  • Roles of AtTPC1, Vacuolar Two Pore Channel 1, in Arabidopsis Stomatal Closure

    Mohammad Mahbub Islam, Shintaro Munemasa, Mohammad Anowar Hossain, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    PLANT AND CELL PHYSIOLOGY   51 ( 2 )   302 - 311   2010年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Abscisic acid (ABA) induces production of reactive oxygen species (ROS) and nitric oxide (NO), elevation of the cytosolic free calcium ion concentration ([Ca(2+)](cyt)) and cytosolic pH (pH(cyt)), and activation of S-type anion channels in guard cells, causing stomatal closure. To investigate whether Arabidopsis Two pore channel 1 (AtTPC1) that encodes the slow vacuolar (SV) channel is involved in stomatal closure, we examined stomatal movements and mobilization of second messengers in the attpc1-2 loss-of-function mutant in response to ABA, methyl jasmonate (MeJA) and Ca(2+). Both ABA and MeJA elicited production of ROS and NO, [Ca(2+)] cyt oscillations, cytosolic alkalization and activation of S-type anion channel currents to lead to stomatal closure in the attpc1-2 mutant as well as the wild type. Unlike the wild type, in the attpc1-2 mutant exogenous Ca(2+) neither induced stomatal closure nor activated plasma membrane S-type anion channel currents despite [Ca(2+)] cyt elevation. These results indicate that AtTPC1 functions in response to external Ca(2+) but not to ABA and MeJA in Arabidopsis guard cells and suggest that AtTPC1 could be involved in priming of plasma membrane S-type anion channels by external Ca(2+) in Arabidopsis guard cells.

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  • A fraction of unripe kiwi fruit extract regulates adipocyte differentiation and function in 3T3-L1 cells

    Daigo Abe, Takeshi Saito, Yasutaka Kubo, Yoshimasa Nakamura, Keizo Sekiya

    BIOFACTORS   36 ( 1 )   52 - 59   2010年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Adipocyte dysfunction is strongly associated with the development of insulin resistance and diabetes, and regulation of adipogenesis is important in prevention of diabetes. We prepared a 100% methanol fraction of methanolic extract from unripe kiwi fruit (Actinidia deliciosa), designated KMF (kiwi fruit methanol fraction) When applied to 3T3-L1 preadipocyte cells, KMF promoted adipocyte differentiation, increased glycerol-3-phosphate dehydrogenase (GPDH) activity, and increased triglyceride (TG) content. KMF markedly increased mRNA expression of peroxisome proliferator-activated receptor gamma (PPAR gamma)-the master adipogenic transcription factor-and its target genes. Moreover, KMF increased mRNA expression and protein secretion of adiponectin, whereas mRNA expression and secretion of monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) were decreased. Compared with troglitazone, KMF decreased the production of reactive oxygen species (ROS) and nuclear factor-kappaB (NF kappa B) activation. Glucose uptake was stimulated by KMF in differentiated 3T3-L1 adipocytes. Taken together, these results indicate that KMF may exert beneficial effects against diabetes via its ability to regulate adipocyte differentiation and function. (C) 2010 International Union of Biochemistry and Molecular Biology, Inc.

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  • Identification and Characterization of Sesaminol Metabolites in the Liver

    Mika Mochizuki, Yoshikazu Tsuchie, Yoshimasa Nakamura, Toshihiko Osawa

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   57 ( 21 )   10429 - 10434   2009年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    Sesame seeds contain a number of antioxidants, such as sesamin, sesamolin, sesaminol, and sesaminol glucosides. Sesaminol triglucoside was reported to suppress oxidative stress in vivo, but little is known about the metabolism of this potentially important compound. Therefore, we have studied the metabolites of sesaminol formed in the rat liver S9 mix and excreted in the liver of rats ingesting sesaminol triglucoside for 24 h. Nuclear magnetic resonance (NMR) and mass spectrometry (MS) analyses revealed that rat liver S9 mix transformed the sesaminol into a catechol-type metabolite. On the basis of a previous study with sesame lignans by culturing the genus Aspergillus, sesaminol-6-catechol was identified as the major metabolite. Sesaminol was further converted into 5 &apos;&apos;-methylated sesaminol-6-catechol by catechol-O-methyltransferase. Moreover, we successfully detected these metabolites in the liver of rats ingesting the sesaminol triglucoside.

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  • Proline and Glycinebetaine Confer Cadmium Tolerance on Tobacco Bright Yellow-2 Cells by Increasing Ascorbate-Glutathione Cycle Enzyme Activities

    Mohammad Muzahidul Islam, Md. Anamul Hoque, Eiji Okuma, Rayhanur Jannat, Mst. Nasrin Akhter Banu, Md. Sarwar Jahan, Yoshimasa Nakamura, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   73 ( 10 )   2320 - 2323   2009年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Cadmium (Cd) stress significantly decreased membrane integrity and impaired the ascorbate (ASC)-glutathione (GSH) cycle in tobacco Bright Yellow-2 cells. Exogenous application of proline and glycinebetaine (betaine) significantly restored the membrane integrity and increased the activities of ASC-GSH cycle enzymes under Cd stress without maintenance of the rich ASC or GSH pools. Moreover, proline offered more efficient protection against Cd stress than betaine.

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  • JNK-dependent NFATc1 pathway positively regulates IL-13 gene expression induced by (-)-epigallocatechin-3-gallate in human basophilic KU812 cells

    Haitao Wu, Hang Qi, Dai Iwasaki, Beiwei Zhu, Yasuaki Shimoishi, Yoshiyuki Murata, Yoshimasa Nakamura

    FREE RADICAL BIOLOGY AND MEDICINE   47 ( 7 )   1028 - 1038   2009年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    (-)-Epigallocatechin-3-gallate (EGCG) has been reported to possess a wide range of biological and pharmacological properties. In this study, we investigated the effects of EGCG on IL-13 gene expression in human basophilic KU812 cells. The IL-13 mRNA expression level was dose-dependently increased by treatment with EGCG (5-20 mu M) for 1 h and additional incubation in a medium for 23 h. EGCG significantly increased the intracellular peroxide level as detected by the peroxide-sensitive probe 2&apos;,7&apos;-dichlorodihydro-fluorescein diacetate. A pharmacological experiment using catalase and a structure-activity relationship study revealed that the exogenously produced H(2)O(2) significantly, but partially, contributed to the IL-13 expression as well as the intracellular oxidative status. Furthermore, EGCG at the concentration required for IL-13 up-regulation activated c-Jun NH(2)-terminal kinase (JNK), but not extracellular signal-regulated protein kinase or p38 mitogen-activated protein kinase in KU812 cells. Transfection of a JNK-specific siRNA as well as treatment with a JNK-specific inhibitor, SP600125, significantly reduced the EGCG-induced IL-13 mRNA expression, by 47.1 and 44.6%, respectively. In addition, we observed the nuclear translocation, mRNA up-regulation, and activation of DNA binding with the IL-13 promoter of nuclear factor of activated T cells (NFATc1) in the EGCG-treated cells. These data provide biological evidence that EGCG induces IL-13 mRNA expression via the JNK-dependent NFATc1 pathway in KU812 cells. (C) 2009 Elsevier Inc. All rights reserved.

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  • Catechol Type Polyphenol Is a Potential Modifier of Protein Sulfhydryls: Development and Application of a New Probe for Understanding the Dietary Polyphenol Actions

    Takeshi Ishii, Miki Ishikawa, Noriyuki Miyoshi, Mayuko Yasunaga, Mitsugu Akagawa, Koji Uchida, Yoshimasa Nakamura

    CHEMICAL RESEARCH IN TOXICOLOGY   22 ( 10 )   1689 - 1698   2009年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    The oxidation of dietary polyphenols with a catechol structure leads to the formation of an o-quinone structure, which rapidly reacts with sulfhydryls such as glutathione and protein cysteine residues. This modification may be important for understanding the redox regulation of cell functions by polyphenols. In this study, to investigate the catechol modification of protein sulthydryls, we used 3,4-dihydroxyphenyl acetic acid (DPA) as a model catechol compound and developed a new probe to directly detect protein modification by catechol type polyphenols using a biotinylated DPA (Bio-DPA). The oxidation-dependent electrophilic reactivity of DPA with peptide sulfhydryls was confirmed by both mass spectrometry and nuclear magnetic resonance spectroscopy. When RL34 cells were treated with Bio-DPA, the significant incorporation of Bio-DPA into a 40 kDa protein was observed by Western blot analysis. The band was identified by mass spectrometry as the cytoskeletal protein, beta-actin. This identification was confirmed by the pull-down assay with anti-beta-actin antibody. To examine the reactivity of the catechol type polyphenols, such as flavonoids, to endogenous beta-actin, RL34 cells were coexposed to Bio-DPA and the flavonoids quercetin, (-)-epicatechin, and (-)-epicatechin gallate. Upon exposure of the cells to Bio-DPA in the presence of the flavonoids, we observed a significant decrease in the DPA-modified beta-actin. These results indicate that beta-actin is one of the major targets of protein modification by catechol type polyphenols and that Bio-DPA is an useful probe for understanding the redox regulation by dietary polyphenols. Furthermore, Keap1, a scaffold protein to the actin cytoskeleton controlling cytoprotective enzyme genes, was also identified as another plausible target of the catechol type polyphenols by oxidative modification of the intracellular sulfhydryls. These results provide an alternative approach to understand that catechol type polyphenol is a potential modifier of redox-dependent cellular events through sulfhydryl modification.

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  • Exogenous Proline and Glycinebetaine Suppress Apoplastic Flow to Reduce Na+ Uptake in Rice Seedlings

    Muhammad Abdus Sobahan, Carlos Raul Arias, Eiji Okuma, Yasuaki Shimoishi, Yoshimasa Nakamura, Yoshihiko Hirai, Izumi C. Mori, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   73 ( 9 )   2037 - 2042   2009年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    The application of exogenous proline and glycinebetaine (betaine) confers salt tolerance on plants under salt stress. The effects of exogenous proline and betaine on apoplastic How in rice plants under saline conditions were investigated using trisodium-8-hydroxy-1,3,6-pyrenetrisulphonic acid (PTS), an apoplastic tracer. Rice plants took up more PTS under light conditions than under dark conditions. Salt stress increased PTS uptake and Na+ content of rice leaves, but did not affect K+ content, resulting in a lower K+/Na+ ratio. Addition of proline or betaine to the saline medium suppressed Na+-induced PTS uptake and Na+ accumulation, while the K+ content was slightly increased, which led to a high K+/Na+ ratio under saline conditions. These results suggest that exogenous proline and betaine suppressed Na+-enhanced apoplastic flow to reduce Na+ uptake in rice plants.

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  • Myrosinases, TGG1 and TGG2, Redundantly Function in ABA and MeJA Signaling in Arabidopsis Guard Cells

    Mohammad Mahbub Islam, Chiharu Tani, Megumi Watanabe-Sugimoto, Misugi Uraji, Md. Sarwar Jahan, Choji Masuda, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    PLANT AND CELL PHYSIOLOGY   50 ( 6 )   1171 - 1175   2009年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Thioglucoside glucohydrolase (myrosinase), TGG1, is a strikingly abundant protein in Arabidopsis guard cells. We investigated responses of tgg1-3, tgg2-1 and tgg1-3 tgg2-1 mutants to abscisic acid (ABA) and methyl jasmonate (MeJA) to clarify whether two myrosinases, TGG1 and TGG2, function during stomatal closure. ABA, MeJA and H2O2 induced stomatal closure in wild type, tgg1-3 and tgg2-1, but failed to induce stomatal closure in tgg1-3 tgg2-1. All mutants and wild type showed Ca-2-induced stomatal closure and ABA-induced reactive oxygen species (ROS)production. A model is discussed in which two myrosinases redundantly function downstream of ROS production and upstream of cytosolic Ca-2 elevation in ABA and MeJA signaling in guard cells.

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  • Induction of apoptosis by β-carotene and dimethyl tetrasulfide assisted by UVA irradiation in HL-60 Cells

    Gongliang Zhang, Gongliang Zhang, Haitao Wu, Haitao Wu, Beiwei Zhu, Yasuaki Shimoishi, Yoshimasa Nakamura, Yoshiyuki Murata

    Bioscience, Biotechnology and Biochemistry   73 ( 5 )   1014 - 1020   2009年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cellular phototoxicity induced by UVA irradiation and its potential application to therapy has been reported. In the present study, the induction of apoptosis induced by β-carotene and dimethyl tetrasulfide (Me2S4) assisted by UVA irradiation in HL-60 cells was assessed. β-carotene assisted by UVA significantly decreased the cell viability and induced DNA fragmentation in HL-60 cells. Me2S4 combined with β-carotene and assisted by UVA significantly inhibited the cell viability, and enhanced the caspase-3 activity which was completely inhibited by N-acety-L-cysteine. β-carotene was significantly degraded by UVA, but this was not accelerated by Me2S4 in a cell culture system. The photodegradation products of β-carotene prepared by UVA irradiation regardless of the addition of Me2S4 showed lower cytotoxicity than β-carotene itself in HL-60 cells. These results suggest that the ROS-and caspase-3-dependent apoptosis induced by β-carotene and Me2S4 assisted by UVA was due to a synergistic action rather than to the sole effect of the photodegradation products of β-carotene in HL-60 cells.

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  • (-)-Epigallocatechin-3-gallate induces up-regulation of Th1 and Th2 cytokine genes in Jurkat T cells

    Haitao Wu, Beiwei Zhu, Yasuaki Shimoishi, Yoshiyuki Murata, Yoshimasa Nakamura

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   483 ( 1 )   99 - 105   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    In the present Study, we found that (-)-epigallocatechin-3-gallate (EGCG) significantly up-regulated the mRNA expression of the Th1/Th2 cytokines including IL-2, IFN-gamma, IL-5 and IL-13 in Jurkat T cells. The EGCG-induced mRNA Up-regulation of IL-2 and IL-5 was predominantly affected by the extracellular signal-regulated protein kinase (ERK) signalling, whereas IL-13 gene expression, the most responsive to the EGCG treatment, was dependent on neither ERK nor c-jun NH(2)-terminal kinase (JNK) signalling. IFN-gamma gene expression was partially mitigated by both inhibitors of the ERK and JNK pathways. Furthermore, catalase significantly attenuated the intracellular peroxide production, phosphorylation of ERK and JNK, and all cytokine gene expressions induced by EGCG. In addition, physiologically relevant concentrations of both EGCG and H(2)O(2)-induced up-regulation of IL-5 gene expression. Our findings provide biological evidence that EGCG induces Th1/Th2 cytokine mRNA expression via H(2)O(2). production followed by activation of ERK or JNK in Jurkat T cells. (C) 2008 Elsevier Inc. All rights reserved.

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  • Docosahexaenoic acid induces ERK1/2 activation and neuritogenesis via intracellular reactive oxygen species production in human neuroblastoma SH-SY5Y cells

    Haitao Wu, Sanae Ichikawa, Chiharu Tani, Beiwei Zhu, Mikiro Tada, Yasuaki Shimoishi, Yoshiyuki Murata, Yoshimasa Nakamura

    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS   1791 ( 1 )   8 - 16   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Docosahexaenoic acid (22: 6n-3: DHA) is a long chain polyunsaturated fatty acid that exists highly enriched in fish oil, and it is one of the low molecular weight food chemicals which can pass a blood brain barrier. A preliminary survey of several fatty acids for expression of growth-associated protein-43 (GAP-43), a marker of axonal growth, identified DHA as one of the most potent inducers. The human neuroblastoma SH-SY5Y cells exposed to DHA showed significant and dose-dependent increases in the percentage of cells with longer neurites. To elucidate signaling mechanisms involved in DHA-enhanced basal neuritogenesis, we examined the role of extracellular signal-regulated kinase (ERK)1/2 and intracellular reactive oxygen species (ROS) production using SH-SY5Y cells. From immunoblotting experiments, we observed that DHA induced the ROS production, protein tyrosine phosphatase inhibition, mitogen-activated protein kinase (MAPK)/ERK kinase (MEK) phosphorylation, and sequentially ERK1/2 phosphorylation, the last of which was significantly reduced by MEK inhibitor U0126. Both antioxidants and MEK inhibitor affected DHA-induced GAP-43 expression, whereas the specific PI3K inhibitor LY294002 did not. We found that total protein tyrosine phosphatase activity was also downregulated by DHA treatment. which was counteracted by antioxidant pretreatment. These results suggest that the ROS-dependent ERK pathway, rather than PI3K, plays an important role during DHA-enhanced neurite outgrowth. (C) 2008 Elsevier B.V. All rights reserved.

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  • Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells

    Mohammad Muzahidul Islam, Anamul Hoque, Eiji Okuma, Mst. Nasrin, Akhter Banu, Yasuaki Shimoishi, Yoshimasa Nakamura, Yoshiyuki Murata

    JOURNAL OF PLANT PHYSIOLOGY   166 ( 15 )   1587 - 1597   2009年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    Environmental stress, including heavy metal stress, can cause oxidative damage to plants. Up-regulation of the antioxidant defense system induced by proline and glycinebetaine (betaine) alleviates the damaging effects of oxidative stress in plants. Here, we investigated the protective effects of exogenously applied proline and betaine on growth, accumulation of proline and betaine, lipid peroxidation and activity of antioxidant enzymes in cultured tobacco Bright Yellow-2 (BY-2) cells exposed to cadmium (Cd) stress. Cadmium stress (at 100 mu M Cd) caused a significant inhibition of the growth of BY-2 cells, and both proline and betaine significantly mitigated this inhibition. In addition, the mitigating effect of proline was more pronounced than that of betaine. Cadmium stress leads to an accumulation of Cd and endogenous proline in cultured cells, increased lipid peroxidation and peroxidase (POX) activity, and decreased activity of superoxide dismutase (SOD) and catalase (CAT). Exogenous application of proline resulted in a decrease in lipid peroxidation and an increase in SOD and CAT activities without reducing Cd contents under Cd stress, while application of betaine resulted in a decrease in lipid peroxidation and an increase in CAT activity with reducing Cd accumulation. Furthermore, exogenous proline and betaine intensified the accumulation of proline and betaine in Cd-stressed BY-2 cells, respectively. The present study suggests that proline and betaine confer tolerance to Cd stress in tobacco BY-2 cells by different mechanisms. (C) 2009 Elsevier GrnbH. All rights reserved.

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  • Proline and glyclinebetaine induce antioxidant defense gene expression and suppress cell death in cultured tobacco cells under salt stress

    Nasrin Akhter Banu, Anamul Hoque, Megumi Watanabe-Sugimoto, Ken Matsuoka, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshlyuki Murata

    JOURNAL OF PLANT PHYSIOLOGY   166 ( 2 )   146 - 156   2009年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    Salt stress causes oxidative damage and cell death in plants. Plants accumulate proline and glycinebetaine (betaine) to mitigate detrimental effects of salt stress. The aim of this study was to investigate the protective effects of proline and betaine on cell death in NaCl-unadapted tobacco (Nicotiana tabacum) Bright Yellow-2 suspension-cultured cells subjected to salt stress. Salt stress increased reactive oxygen species (ROS) accumulation, lipid peroxidation, nuclear deformation and degradation, chromatin condensation, apoptosis-tike cell death and ATP contents. Neither proline nor betaine affected apoptosis-like cell death and G, phase population, and increased ATP contents in the 200 mM NaCl-stressed cells. However, both of them effectively decreased ROS accumulation and lipid peroxidation, and suppressed nuclear deformation and chromatin condensation induced by severe salt stress. Evans Blue staining experiment showed that both proline and betaine significantly suppressed increment of membrane permeability induced by 200 mM NaCl. Furthermore, among the ROS scavenging antioxidant defense genes studied here, mRNA levels of salicylic acid-binding (SAbind) catalase (CAT) and lignin-forming peroxidase (POX) were found to be increased by proline and betaine under salt stress. It is concluded that both proline and betaine provide a protection against NaCl-induced cell death via decreasing level of ROS accumulation and lipid peroxidation as well as improvement of membrane integrity. (C) 2008 Elsevier GmbH. ALL rights reserved.

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  • Nitric oxide functions in both methyl jasmonate signaling and abscisic acid signaling in Arabidopsis guard cells

    Naoki Saito, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

    Plant Signaling and Behavior   4 ( 2 )   119 - 120   2009年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Landes Bioscience  

    Intracellular components in methyl jasmonate (MeJA) signaling remain largely unknown, to compare those in well-understood abscisic acid (ABA) signaling. We have reported that nitric oxide (NO) is a signaling component in MeJA-induced stomatal closure, as well as ABA-induced stomatal closure in the previous study. To gain further information about the role of NO in the guard cell signaling, NO production was examined in an ABA- and MeJA-insensitive Arabidopsis mutant, rcn1. Neither MeJA nor ABA induced NO production in rcn1 guard cells. Our data suggest that NO functions downstream of the branch point of MeJA and ABA signaling in Arabidopsis guard cells. ©2009 Landes Bioscience.

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  • Chemoprevention by Isothiocyanates: Molecular Basis of Apoptosis Induction

    Yoshimasa Nakamura

    FOOD FACTORS FOR HEALTH PROMOTION   61   170 - 181   2009年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    An important and promising group of compounds that have a cancer-chemopreventive property are organosulfur compounds, such as isothiocyanates (ITCs). Various ITCs are effective chemoprotective agents against chemical carcinogenesis in experimental animals. Several epidemiological studies also indicated that the dietary consumption of ITCs or ITC-containing foods inversely correlates with the risk of developing lung, breast, and colon cancers, providing evidence that they have a potential to prevent cancer in humans. Mechanistically, ITCs are capable of inhibiting both the formation and development of a cancer cell through multiple pathways; i.e. the inhibition of carcinogen-activating cytochrome P450 mono-oxygenases, induction of carcinogen-detoxifying phase 2 enzymes, induction of apoptosis, and inhibition of cell cycle progression. We have clarified the molecular mechanisms underlying the relationship between cell cycle regulation and apoptosis induced by benzyl ITC (BITC), a major ITC compound isolated from papaya (Corica papaya) fruit. We identified phosphorylated Bcl-2 as a key molecule linking p38 MAPK-dependent cell cycle regulation with the c-Jun N-terminal kinase activation by BITC. We also established that BITC exerts the cytotoxic effect more preferentially in the proliferating cells than in the quiescent cells. Furthermore, p53 was found to be a potential negative regulator of apoptosis induction by BITC in normal epithelial cells through inhibition of cell cycle progression at the G(0)/G(1) phase. In contrast, treatment with an excessive concentration of BITC resulted in necrotic cell death in an ATP-dependent manner. This review addresses the biological impact of cell death induction by BITC as well as other ITCs and the involved molecules regulating signal pathways. Copyright (C) 2009 S. Karger AG, Basel

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  • Extensive skin necrosis of the arm in a patient with complex regional pain syndrome 査読

    M. Nishikawa, M. Tanioka, E. Araki, Y. Matsumura, S. Kore-Eda, Y. Nakamura, K. Nose, A. Utani, Y. Miyachi

    Clinical and Experimental Dermatology   33 ( 6 )   733 - 735   2008年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We report a 36-year-old woman with complex regional pain syndrome (CRPS) type 1 presenting with extensive skin necrosis of the left arm. The patient cooled her arm with ice packs to ease severe pain due to CRPS, in spite of repeated cautions against frostbite injury. The regions of skin necrosis corresponded with the sites where she had applied ice packs. We considered that the severe skin necrosis in our case was due to a self-induced frostbite injury. © 2008 The Author(s).

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  • Effect of Dimethyl Sulfides on the Induction of Apoptosis in Human Leukemia Jurkat Cells and HL-60 Cells

    Gongliang Zhang, Haitao Wu, Beiwei Zhu, Yasuaki Shimoishi, Yoshimasa Nakamura, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   72 ( 11 )   2966 - 2972   2008年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Organosulfur compounds have been established to possess anticancer effects. To provide a better understanding of the biological function of dimethyl sulfides, dimethyl monosulfide (Me2S), dimethyl disulfide (Me2S2), dimethyl trisulfide (Me2S3) and dimethyl tetrasulfide (Me2S4) were used as experimental materials to investigate their effects on apoptosis induction in human leukemia Jurkat cells and HL-60 cells. Treatment with 20 Km dimethyl sulfides for 24 h decreased the viability of both cells. The cell viability-reducing effect of these sulfides was in the following order: Me2S4 approximate to Me2S3 &gt; Me2S2 approximate to Me2S for Jurkat cells and Me2S4 &gt; Me2S3 &gt; Me2S2 approximate to Me2S for HL-60 cells. Me2S3 and Me2S4 significantly induced DNA fragmentation and caspase-3 activation. The addition of GSH or NAC completely suppressed the sulfide-induced apoptosis. Our results indicate that dimethyl sulfides with a larger number of sulfur atoms more strongly induced apoptosis in both human leukemia cells via ROS production and caspase-3 activation.

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  • (-)-Epigallocatechin-3-gallate Potentiates the Cytotoxicity Induced by Benzyl Isothiocyanate and Hydrogen Peroxide in Human Jurkat T Lymphocytes

    Haitao Wu, Tomoko Yokoyama, Beiwei Zhu, Yasuaki Shimoishi, Yoshiyuki Murata, Yoshimasa Nakamura

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   72 ( 11 )   3034 - 3037   2008年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    (-)-Epigallocatechin-3-gallate (EGCG)-induced apoptosis was along both the extracellular signal-regulated protein kinase (ERK) and c-jun N-terminal kinase (JNK) pathways in Jurkat cells. Co-treatment with EGCG potentiated the cytotoxicity induced by benzyl isothiocyanate (BITC) and H2O2, both being inhibited by ERK and JNK inhibitors. These results suggest the significant role of mitogen-activated protein kinase (MAPK) signaling in the apoptosis induction regulated by EGCG alone and in combination.

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  • ATP depletion alters the mode of cell death induced by benzyl isothiocyanate

    Noriyuki Miyoshi, Etsuko Watanabe, Toshihiko Osawa, Masashi Okuhira, Yoshiyuki Murata, Hiroshi Ohshima, Yoshimasa Nakamura

    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE   1782 ( 10 )   566 - 573   2008年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Pro-inflammatory death is presumably an undesirable event in cancer prevention process, thus biochemical comprehension and molecular definition of this process could have important clinical implications. In the present study, we examined the cytophysiological conversion of cell death mode by benzyl isothiocyanate (BITC) in human cervical cancer HeLa cells. The detailed studies using flow cytometric and morphological analyses demonstrated that the cells treated with appropriate concentration (25 mu M) of BITC showed apoptotic feature, such as chromatin condensation, DNA fragmentation, and preserved plasma membrane integrity, whereas these features were disappeared by treatment with higher concentration (100 mu M). The treatment with 2-deoxyglucose, an inhibitor of ATP synthesis, drastically increased in the ratio of necrotic dead cells, while it influences little that of apoptotic cells. Moreover, an analysis using the mitochondrial DNA-deficient HeLa cells demonstrated that the rho degrees cells were more susceptible to the BITC-induced necrosis-like cell death compared to the wild-type (rho(+)) cells, whereas the ROS production was significantly inhibited in the rho degrees cells. It is likely that the BITC-induced ROS is derived from mitochondrial respiratory chain and ruled out the contribution to the mechanism of cell death mode switching. In addition, the BITC treatment resulted in a more rapid depletion of ATP in the rho degrees cells than in the rho(+) cells. Furthermore. a caspase inhibitor, Z-VAD-fmk counteracted not only apoptosis, but also necrosis-like cell death induced by BITC, suggesting that increment in this cell death pattern might be due to the interruption of events downstream of a caspase-dependent pathway. The obtained data suggested that the decline in the intracellular ATP level plays an important role in tuning the mode of cell death by BITC. (C) 2008 Elsevier B.V. All rights reserved.

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  • ATP depletion alters the mode of cell death induced by benzyl isothiocyanate. 査読

    Miyoshi N, Watanabe E, Osawa T, Okuhira M, Murata Y, Ohshima H, Nakamura Y

    Biochimica et biophysica acta   1782 ( 10 )   566 - 573   2008年10月

  • Deficient Glutathione in Guard Cells Facilitates Abscisic Acid-Induced Stomatal Closure but Does Not Affect Light-Induced Stomatal Opening

    Md. Sarwar Jahan, Ken'ichi Ogawa, Yoshimasa Nakamura, Yasuaki Shimoishi, Izumi C. Mori, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   72 ( 10 )   2795 - 2798   2008年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    We investigated the role of glutathione (GSH) in stomatal movements using a GSH deficient mutant, chlorinal-1 (ch1-1). Guard cells of ch1-1 mutants accumulated less GSH than wild types did. Light induced stomatal opening in ch1-1 and wild-type plants. Abscisic acid (ABA) induced stomatal closure in ch1-1 mutants more than wild types without enhanced reactive oxygen species (ROS) production. Therefore, GSH functioned downstream of ROS production in the ABA signaling cascade.

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  • Roles of RCN1, regulatory a subunit of protein phosphatase 2A, in methyl jasmonate signaling and signal crosstalk between methyl jasmonate and abscisic acid

    Naoki Saito, Shintaro Munemasa, Yoshimasa Nakamura, Yasuaki Shimoishi, Izumi C. Mori, Yoshiyuki Murata

    PLANT AND CELL PHYSIOLOGY   49 ( 9 )   1396 - 1401   2008年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Methyl jasmonate (MeJA) as well as abscisic acid (ABA) induces stomatal closure with their signal crosstalk. We investigated the function of a regulatory A subunit of protein phosphatase 2A, RCN1, in MeJA signaling. Both MeJA and ABA failed to induce stomatal closure in Arabidopsis rcn1 knockout mutants unlike in wild-type plants. Neither MeJA nor ABA induced reactive oxygen species (ROS) production and suppressed inward-rectifying potassium channel activities in rcn1 mutants but not in wild-type plants. These results suggest that RCN1 functions upstream of ROS production and downstream of the branch point of MeJA signaling and ABA signaling in Arabidopsis guard cells.

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  • Structure-dependent photodegradation of carotenoids accelerated by dimethyl tetrasulfide under UVA irradiation

    Gongliang Zhang, Beiwei Zhu, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   72 ( 8 )   2176 - 2183   2008年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Carotenoids are used in wide-ranging food applications, but they are susceptible to degradation by many factors including light. We examined the photodegradation of five kinds of carotenoids and three kinds of anthocyanins to clarify which structures of pigments were favorable to accelerated degradation by sulfides under UVA irradiation. Under UVA irradiation, crocetin and crocin were decomposed more rapidly in the presence of dimethyl tetrasulfide than in the absence of the sulfide, but not as rapidly as beta-carotene, zeaxanthin and beta-cryptoxanthin were. However, cyanidin was decomposed more slowly in the presence of sulfide than in the absence of sulfide. Moreover, the photodegradation of kuromanin and keracyanin was not affected by the addition of a sulfide. We also examined the mechanism for this accelerated degradation. Normal hexane was more favorable to the photodegradation of beta-carotene than methanol and ethanol. The accelerated degradation was inhibited by free radical scavengers, but enhanced by the addition of deuterium oxide. These results suggest that conjugated double bonds were favorable to the accelerated photodegradation by sulfide and that this reaction was mediated by free radicals.

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  • Reactive oxygen species and angiogenesis: NADPH oxidase as target for cancer therapy

    Masuko Ushio-Fukai, Yoshimasa Nakamura

    CANCER LETTERS   266 ( 1 )   37 - 52   2008年7月

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    記述言語:英語   出版者・発行元:ELSEVIER IRELAND LTD  

    Angiogenesis is essential for tumor growth, metastasis, arteriosclerosis as well as embryonic development and wound healing. Its process is dependent on cell proliferation, migration and capillary tube formation in endothelia cells (ECs). High levels of reactive oxygen species (ROS) such as superoxide and H(2)O(2) are observed in various cancer cells. Accumulating evidence suggests that ROS function as signaling molecules to mediate various growth-related responses including angiogenesis. ROS-dependent angiogenesis can be regulated by endogenous antioxidant enzymes such as SOD and thioredoxin. Vascular endothelial growth factor (VEGF), one of the major angiogenesis factor, is induced in growing tumors and stimulates EC proliferation and migration primarily through the VEGF receptor type2 (VEGFR2, Flk1/KDR). Major source of ROS in ECs is a NADPH oxidase which consists of Nox1, Nox2, Nox4, Nox5, p22(phox), p47 (phox) and the small G-protein Rac1. NADPH oxidase is activated by various growth factors including VEGF and angiopoietin-1 as well as hypoxia and ischemia, and ROS derived from this oxidase are involved in VEGFR2 autophosphorylation, and diverse redox signaling pathways leading to induction of transcription factors and genes involved in angiogenesis. Dietary antioxidants appear to be effective for treatment of tumor angiogenesis. The aim of this review is to provide an overview of the recent progress on role of ROS derived from NADPH oxidase and redox signaling events involved in angiogenesis. Understanding these mechanisms may provide insight into the NADPH oxidase and redox signaling components as potential therapeutic targets for tumor angiogenesis (c) 2008 Elsevier Ireland Ltd. All rights reserved.

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  • Galloylated catechins as potent inhibitors of hypochlorous acid-induced DNA damage

    Yoshichika Kawai, Yuri Matsui, Hajime Kondo, Hiroshi Morinaga, Koji Uchida, Noriyuki Miyoshi, Yoshimasa Nakamura, Toshihiko Osawa

    CHEMICAL RESEARCH IN TOXICOLOGY   21 ( 7 )   1407 - 1414   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    Hypochlorous acid (HOC1), a strong oxidant derived from myeloperoxidase in neutrophils and macrophages, can chlorinate DNA bases at the site of inflammation. Because little is known about the protective role of natural antioxidants, such as polyphenols, for the myeloperoxidase-derived DNA damage, we screened the inhibitory effects of various phenolic antioxidants on the chlorination of the 2'-deoxycytidine residue by HOC1 in vitro and found that green tea catechins, especially (-)-epicatechin gallate (ECg) and (-)-epigallocatechin gallate (EGCg), significantly inhibited the chlorination. These catechins also reduced nucleoside- and taurine-chloramines, which can induce secondary oxidative damage, into their native forms. Mass spectrometric and nuclear magnetic resonance analyses showed that ECg and EGCg can effectively scavenge HOC1 and/or chloramine species resulting in the formation of mono-and dichlorinated ECg and EGCg. Using the HL-60 human leukemia cell line, it was found that ECg could efficiently accumulate in the cells. Immunocytometric analyses using antihalogenated 2'-deoxycytidine antibody showed that pretreatment of cells with ECg inhibited the HOC1-induced immunofluorescence. In addition, the chlorinated ECg derivatives were detected in the HOC1-treated HL-60 cells. These results showed that green tea catechins, especially 3-galloylated catechins, may be the plausible candidate for the prevention of inflammation-derived DNA damage and perhaps carcinogenesis.

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  • Role of protein tyrosine phosphatase 1B in vascular endothelial growth factor signaling and cell-cell adhesions in endothelial cells

    Yoshimasa Nakamura, Nikolay Patrushev, Hyoe Inomata, Dolly Mehta, Norifumi Urao, Ha Won Kim, Masooma Razvi, Vidisha Kini, Kalyankar Mahadev, Barry J. Goldstein, Ronald McKinney, Tohru Fukai, Masuko Ushio-Fukai

    CIRCULATION RESEARCH   102 ( 10 )   1182 - 1191   2008年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Vascular endothelial growth factor (VEGF) binding induces phosphorylation of VEGF receptor (VEGFR) 2 in tyrosine, which is followed by disruption of VE-cadherin-mediated cell-cell contacts of endothelial cells (ECs), thereby stimulating EC proliferation and migration to promote angiogenesis. Tyrosine phosphorylation events are controlled by the balance of activation of protein tyrosine kinases and protein tyrosine phosphatases (PTPs). Little is known about the role of endogenous PTPs in VEGF signaling in ECs. In this study, we found that PTP1B expression and activity are markedly increased in mice hindlimb ischemia model of angiogenesis. In ECs, overexpression of PTP1B, but not catalytically inactive mutant PTP1B-C/S, inhibits VEGF-induced phosphorylation of VEGFR2 and extracellular signal-regulated kinase 1/2, as well as EC proliferation, whereas knockdown of PTP1B by small interfering RNA enhances these responses, suggesting that PTP1B negatively regulates VEGFR2 signaling in ECs. VEGF-induced p38 mitogen-activated protein kinase phosphorylation and EC migration are not affected by PTP1B overexpression or knockdown. In vivo dephosphorylation and cotransfection assays reveal that PTP1B binds to VEGFR2 cytoplasmic domain in vivo and directly dephosphorylates activated VEGFR2 immunoprecipitates from human umbilical vein endothelial cells. Overexpression of PTP1B stabilizes VE-cadherin-mediated cell-cell adhesions by reducing VE-cadherin tyrosine phosphorylation, whereas PTP1B small interfering RNA causes opposite effects with increasing endothelial permeability, as measured by transendothelial electric resistance. In summary, PTP1B negatively regulates VEGFR2 receptor activation via binding to the VEGFR2, as well as stabilizes cell-cell adhesions through reducing tyrosine phosphorylation of VE-cadherin. Induction of PTP1B by hindlimb ischemia may represent an important counterregulatory mechanism that blunts overactivation of VEGFR2 during angiogenesis in vivo.

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  • Alpha-tocopherol induces procaspase-3 expression mediated by the transcriptional factor Sp1 査読

    Miyoshi Noriyuki, Nakamura Yoshimasa, Ohshima Hiroshi

    FASEB JOURNAL   22   2008年4月

  • Proline and glycinebetaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaCl-induced damage in cultured tobacco cells

    Md. Anamul Hoque, Mst. Nasrin Akhter Banu, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata

    JOURNAL OF PLANT PHYSIOLOGY   165 ( 8 )   813 - 824   2008年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    Salt stress impairs reactive oxygen species (ROS) and methylglyoxal (MG) detoxification systems, and causes oxidative damage to plants. Up-regulation of the antioxidant and glyoxalase systems provides protection against NaCl-induced oxidative damage in plants. Thiol-disulfide contents, glutathione content and its associated enzyme activities involved in the antioxidant defense and glyoxalase systems, and protein carbonylation in tobacco Bright Yellow-2 cells grown in suspension culture were investigated to assess the protection offered by proline and glycinebetaine against salt stress. Salt stress increased protein carbonylation, contents of thiol, disulfide, reduced (GSH) and oxidized (GSSG) forms of glutathione, and the activity of glutathione-S-transferase and glyoxalase II enzymes, but decreased redox state of both thiol-disulfide and glutathione, and the activity of glutathione peroxidase and glyoxalase I enzymes involved in the ROS and MG detoxification systems. Exogenous application of proline or glycinebetaine resulted in a reduction of protein carbonylation, and in an increase in glutathione redox state and activity of glutathione peroxidase, glutathione-S-transferase and glyoxalase I under salt stress. Neither proline noi glycinebetaine, however, had any direct protective effect on NaCl-induced GSII-associated enzyme activities. The present study, therefore, suggests that both proline and glycinebetaine provide a protective action against NaCl-induced oxidative damage by reducing protein carbonylation, and enhancing antioxidant defense and MG detoxification systems. (C) 2007 Elsevier GmbH. All rights reserved.

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  • Exogenous proline and glycinebetaine increase NaCl-induced a scorbate-glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspension-cu ltured cells

    Md. Anamul Hoque, Mst. Nasrin Akhter Banu, Eiji Okuma, Yoshiyuki Murata

    JOURNAL OF PLANT PHYSIOLOGY   164 ( 11 )   1457 - 1468   2007年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    Up-regulation of the antioxidant system provides protection against NaCl-induced oxidative damage in plants. Antioxidants and activity of enzymes involved in the ascorbate-glutathione (ASC-GSH) cycle in tobacco Bright Yellow-2 (BY-2) were investigated to assess the antioxidant protection offered by exogenous proline and glycinebetaine (betaine from now on) against salt stress using cells grown in suspension culture. Reduced ascorbate (ASC) was detected in BY-2 cells but dehydroascorbate (DHA) was not. Large quantities of a reduced form of glutathione (GSH) and smaller quantities of an oxidized form of glutathione (GSSG) were detected in BY-2 cells. Salt stress significantly reduced the contents of ASC and GSH as well. as activities of ASC-GSH cycle enzymes such as ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR). Exogenous proline or betaine increased the activities of all enzymes except MDHAR involved in NaCl-induced ASC-GSH cycle. Levels of ASC and GSH in BY-2 cells under salt stress were lower in the presence of proline or betaine than in the absence of proline or betaine whereas there was no difference in redox status. Proline proved more effective than betaine in maintaining the activity of enzymes involved in NaCl-induced ASC-GSH cycle. Neither proline nor betaine had any direct protective effect on NaCl-induced enzyme activity involved in the antioxidant system; however, both improved salt tolerance by increasing enzyme activity. The present study, together with our earlier findings [Hoque MA, Okuma E, Banu MNA, Nakamura Y, Shimoishi Y, Murata Y. Exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine by increasing antioxidant enzyme activities. J Plant Physiol 2006;164:553-61.], suggests that proline offered greater protection against salt stress than betaine did because proline was more effective in increasing the activity of enzymes involved in the antioxidant system. (C) 2006 Elsevier GmbH. All rights reserved.

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  • Dietary flavonoid apigenin is a potential inducer of intracellular oxidative stress: The role in the interruptive apoptotic signal

    Noriyuki Miyoshi, Kisa Naniwa, Takayo Yamada, Toshihiko Osawa, Yoshimasa Nakamura

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   466 ( 2 )   274 - 282   2007年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Apigenin is a representative dietary flavone (2-phenyl-4H-1-benzopyran-4-one) inhibiting cancer cell growth both in cell culture systems and in vivo. The prooxidant potential of apigenin was confirmed by the observations using flowcytometric and irnmunoblotting techniques that the intracellular accumulations of reactive oxygen species (ROS) and protein carbonyls were detected in the cells treated, with apigenin in a dose-dependent manner. Conversely, chrysin (5,7-dihydroxyflavone) did not show any prooxidant effect. A structure-activity relationship data thus indicated that a 4'-monohydroxyl group, which can be oxidized to semiquinone radical but not up to quinone-like metabolite, is essential for prooxidant effect. When HL-60 cells were treated with not only a heme synthesis inhibitor succinyl acetone (SA) but also myeloperoxidase (MPO) inhibitors, the ROS level enhanced by apigenin was significantly reduced. The gathered data suggested that peroxidase-catalyzed production of apigenin B-ring phenoxyl radicals might be responsible for the prooxidant effect. This is supported by the observation that MPO is able to catalyze production of apigenin phenoxyl radicals, detected by an electron spin resonance-spin trapping technique. We also reveal that both SA and a-tocopherol enhance cellular susceptibility to apoptosis-inducing stimuli by apigenin. In conclusion, the prooxidant effect of apigenin is likely to oxidize a variety of thiols through the formation of phenoxyl radicals and thus seems to play a significant role in the abortive apoptotic pathway switching to necrotic cell death. (c) 2007 Elsevier Inc. All rights reserved.

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  • Exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine by increasing antioxidant enzyme activities

    Md. Anamul Hoque, Eiji Okuma, Mst. Nasrin Akhter Banu, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata

    JOURNAL OF PLANT PHYSIOLOGY   164 ( 5 )   553 - 561   2007年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH, URBAN & FISCHER VERLAG  

    Proline and betaine accumulate in plant cells under environmental stresses including stress. Here, we investigated effects of proline and betaine on the growth and activities of antioxidant enzymes in tobacco Bright Yellow-2 (BY-2) culture cells in suspension under salt stress. Both proline and betaine mitigated the inhibition of growth of BY-2 cells under salt stress and the mitigating effect of proline was more than that of betaine. Salt stress significantly decreased the activities of superoxide dismutase (SOD), catalase and peroxidase in BY-2 cells. Exogenous application of proline or betaine alleviated the reduction in catalase and peroxidase activities but not SOD activity under salt stress. In addition, proline was found to be effective in alleviating the inhibition of salt stress-induced catalase and peroxidase activities in BY-2 cells. Neither proline nor betaine directly scavenged superoxide (02) or hydrogen peroxide (H2O2). It is concluded that exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine because of its superior ability to increase the activities of antioxidant enzymes. (C) 2006 Elsevier GmbH. All rights reserved.

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  • Detection of modified tyrosines as an inflammation marker in a photo-aged skin model

    Yukiko Ishitsuka, Fumio Maniwa, Chiharu Koide, Natsuko Douzaki, Yoji Kato, Yoshimasa Nakamura, Toshihiko Osawa

    PHOTOCHEMISTRY AND PHOTOBIOLOGY   83 ( 3 )   698 - 705   2007年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PHOTOBIOLOGY  

    Reactive nitrogen species, produced during the process of inflammation induced by various factors including UV radiation, modify amino acids in crucial proteins. It is assumed that skin tissue is more likely to be modified, as it is located at the outer layer of a body that is exposed to UV radiation on a daily basis. To investigate the influence of the modified tyrosine on UV-exposed skin, we detected the nitrotyrosine or halogenated tyrosine and dityrosine in photo-aged model mice. The back skin of mice was exposed to a dose of 10 J cm(-2) day(-1) every day for 15 weeks. Samples exhibiting typical symptoms of photo aging were provided to the immunofluorescence study. The quantification of modified proteins was accomplished through a chemical analytical method known as HPLC-tandem mass spectrometry. Analysis of the irradiated skin samples showed that all modified tyrosine except nitrotyrosine demonstrated statistically significant increases. The molecular weights of major modified proteins, confirmed as 25-50 kDa, were measured using Western blot analysis with an anti-nitrotyrosine antibody. Furthermore, the immunofluorescence study verified that the localization of myeloperoxidase conformed to that of nitrotyrosine. This result suggests that the modified tyrosine was produced during the process of inflammation by UV irradiation. In this study, we used a low dose of UV irradiation to which we are exposed in daily life. Our results suggest that UV exposure in daily life may induce the production of modified tyrosines and skin aging.

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  • Papaya seed represents a rich source of biologically active isothiocyanate

    Yoshimasa Nakamura, Motoko Yoshimoto, Yoshiyuki Murata, Yasuaki Shimoishi, Yumi Asai, Eun Young Park, Kenji Sato, Yasushi Nakamura

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   55 ( 11 )   4407 - 4413   2007年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    In the present study, papaya (Carica papaya) seed and edible pulp were carefully separated and then the contents of benzyl isothiocyanate and the corresponding glucosinolate (benzyl glucosinolate, glucotropaeolin) quantified in each part. The papaya seed with myrosinase inactivation contained &gt; 1 mmol of benzyl glucosinolate in 100 g of fresh seed. This content is equivalent to that of Karami daikon (the hottest Japanese white radish) or that of cress. The papaya seed extract also showed a very high activity of myrosinase and, without myrosinase inactivation, produced 460 mu mol of benzyl isothiocyanate in 100 g of seed. In contrast, papaya pulp contained an undetectable amount of benzyl glucosinolate and showed no significant myrosinase activity. The n-hexane extract of the papaya seed homogenate was highly effective in inhibiting superoxide generation and apoptosis induction in HL-60 cells, the activities of which are comparable to those of authentic benzyl isothiocyanate.

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  • The coronatine-insensitive 1 mutation reveals the hormonal signaling interaction between abscisic acid and methyl jasmonate in arabidopsis guard cells. Specific impairment of ion channel activation and second messenger production

    Shintaro Munemasa, Kenji Oda, Megumi Watanabe-Sugimoto, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata

    PLANT PHYSIOLOGY   143 ( 3 )   1398 - 1407   2007年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Methyl jasmonate (MeJA) elicits stomatal closing similar to abscisic acid (ABA), but whether the two compounds use similar or different signaling mechanisms in guard cells remains to be clarified. We investigated the effects of MeJA and ABA on second messenger production and ion channel activation in guard cells of wild-type Arabidopsis ( Arabidopsis thaliana) and MeJA-insensitive coronatine-insensitive 1 (coi1) mutants. The coi1 mutation impaired MeJA-induced stomatal closing but not ABA-induced stomatal closing. MeJA as well as ABA induced production of reactive oxygen species (ROS) and nitric oxide (NO) in wild-type guard cells, whereas MeJA did not induce production of ROS and NO in coi1 guard cells. The experiments using an inhibitor and scavengers demonstrated that both ROS and NO are involved in MeJA-induced stomatal closing as well as ABA-induced stomatal closing. Not only ABA but also MeJA activated slow anion channels and Ca2+ permeable cation channels in the plasma membrane of wild-type guard cell protoplasts. However, in coi1 guard cell protoplasts, MeJA did not elicit either slow anion currents or Ca2+ permeable cation currents, but ABA activated both types of ion channels. Furthermore, to elucidate signaling interaction between ABA and MeJA in guard cells, we examined MeJA signaling in ABA-insensitive mutant ABA-insensitive 2 (abi2-1), whose ABA signal transduction cascade has some disruption downstream of ROS production and NO production. MeJA also did not induce stomatal closing but stimulated production of ROS and NO in abi2-1. These results suggest that MeJA triggers stomatal closing via a receptor distinct from the ABA receptor and that the coi1 mutation disrupts MeJA signaling upstream of the blanch point of ABA signaling and MeJA signaling in Arabidopsis guard cells.

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  • Selective cytotoxicity of benzyl isothiocyanate in the proliferating fibroblastoid cells

    Noriyuki Miyoshi, Koji Uchida, Toshihiko Osawa, Yoshimasa Nakamura

    INTERNATIONAL JOURNAL OF CANCER   120 ( 3 )   484 - 492   2007年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    In the present study, experiments using presynchronization culture cells demonstrated that benzyl ITC (BITC), previously isolated from a tropical papaya fruit extract, induced the cytotoxic effect preferentially in the proliferating human colon CCD-18Co cells to the quiescent ones. Quiescent CCD-18Co cells were virtually unaffected by BITC and marginal cytotoxicity was observed at 15 mu M. We observed that BITC dramatically induced the p53 phosphorylation and stabilization only in the quiescent (G(0)/G(1) phase-arrested) cells, but not significantly in the proliferating human colon CCD-18Co cells when compared with quiescent ones. We also observed ataxia telangiectasia-mutated (ATM) phosphorylation in the quiescent cells. The BITC-induced p53 phosphorylation was counteracted by caffeine treatment, implying the involvement of an ATM/ataxia telangiectasia and Rad3-related kinase signaling pathway. Moreover, downregulation of p53 by a siRNA resulted in the enhancement of susceptibility to undergo apoptosis by BITC. We also showed here that depletion of p53 abrogated G(0)/G(1), arrest accompanied by the declined expression of p21(waf1/cip1) and p27(kip1) in CCD-18Co cells. In conclusion, we identified p53 as a potential negative regulator of the apoptosis induction by BITC in the normal colon CCD-18Co cells through the inhibition of cell-cycle progression at the G(0)/G(1) phase. (c) 2006 Wiley-Liss, Inc.

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  • Molecular mechanism underlying the relationship between cell cycle arrest and apoptosis induced by benzyl isothiocyanate 査読

    Miyoshi Noriyuki, Nakamura Yoshimasa, Ohshima Hiroshi

    YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN   127   145 - 146   2007年

  • Expression and Ca2+ dependency of plasma membrane K+ channels of tobacco suspension cells adapted to salt stress

    Naoki Kasukabe, Megumi Watanabe-Sugimoto, Ken Matsuoka, Eiji Okuma, Ichiro Obi, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata, Tadaaki Kakutani

    PLANT AND CELL PHYSIOLOGY   47 ( 12 )   1674 - 1677   2006年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The expression of plasma membrane K+ channels of NaCl-adapted tobacco suspension cells and effects of extracellular Ca2+ on plasma membrane K+ channels were investigated. Reverse transcription-PCR (RT-PCR) analysis showed that expression of TORK1, which encodes a K+ channel, was much lower in NaCl-adapted cells than in NaCl-unadapted cells. The magnitude of the outward K+ currents of NaCl-adapted as well as NaCl-unadapted cells decreased with increasing extracellular Ca2+ but there is no significant difference in Ca2+ dependency of the K+ current. These analyses suggest that reduction of the number of K+ channels might cause NaCl adaptation of cells through the decrease of outward K+ currents.

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  • A novel labdane-type trialdehyde from Myoga (Zingiber mioga Roscoe) that potently inhibits human platelet aggregation and human 5-lipoxygenase

    Masako Abe, Yoshio Ozawa, Yasushi Uda, Yasujiro Morimitsu, Yoshimasa Nakamura, Toshihiko Osawa

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   70 ( 10 )   2494 - 2500   2006年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    We screened myoga extracts for inhibitors of human platelet aggregation and human 5-lipoxygenase. We identified a novel labdane type of diterpene, together with three known diterpenes (miogadial and galanals A and B) from the flower buds of myoga. Spectroscopic data indicated the structure of the new compound to be 12(E)-labdene-15,16,(8 beta)17-trial (miogatrial). Miogatrial and miogadial were potent inhibitors of human platelet aggregation and human 5-lipoxygenase (5-LOX). The sesquiterpene, polygodial, also exhibited strong inhibitory activity against human platelet aggregation and 5-LOX. On the other hand, galanals A and B did not have inhibitory activity in either experimental system. It thus appears that a 3-formyl-3-butenal structure was essential for the potent inhibition of human platelet aggregation and human 5-LOX.

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  • Cell death induction by isothiocyanates and their underlying molecular mechanisms

    Yoshimasa Nakamura, Noriyuki Miyoshi

    BIOFACTORS   26 ( 2 )   123 - 134   2006年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IOS PRESS  

    An important and promising group of compounds that have a chemopreventive property are organosulfur compounds, such as isothiocyanates (ITCs). In recent years, it has been shown that ITCs induce apoptosis in various cancer cell lines and experimental rodents. During the course of apoptosis induction by ITC, multiple signal-transduction pathways and apoptosis intermediates are modulated. We have also clarified the molecular mechanism underlying the relationship between cell cycle arrest and apoptosis induced by benzyl isothiocyanate ( BITC), a major ITC compound isolated from papaya. The exposure of cells to BITC resulted in the inhibition of the G(2)/M progression that coincided with not only the up-regulated expression of the G(2)/M cell cycle arrest-regulating genes but also the apoptosis induction. The experiment using the phase-specific synchronized cells demonstrated that the G(2)/M phase-arrested cells are more sensitive to undergoing apoptotic stimulation by BITC than the cells in other phases. We identified the phosphorylated Bcl-2 as a key molecule linking the p38 MAPK-dependent cell cycle arrest with the JNK activation by BITC. We also found that BITC induced the cytotoxic effect more preferentially in the proliferating normal human colon epithelial cells than in the quiescent cells. Conversely, treatment with an excessive concentration of BITC resulted in necrotic cell death without DNA ladder formation. This review addresses the biological impact of cell death induction by BITC as well as other ITCs and the involved signal transduction pathways.

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  • Dietary isothiocyanates modify mitochondrial functions through their electrophilic reaction

    M Kawakami, N Harada, M Hiratsuka, K Kawai, Y Nakamura

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   69 ( 12 )   2439 - 2444   2005年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    We found that both benzyl isothiocyanate (ITC) and phenyl ITC inhibited respiration in the mitochondria in an electrophilic reaction-dependent manner. ITG induced mitochondrial swelling and cytochrome c release were prevented by cyclosporin A, indicating that they are mediated through the ITC moiety-dependent reaction to critical thiol groups for the opening of membrane permeability transition-dependent pores.

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  • A Fermented Grain Food Product Extract Ameliorates Tumor Promoter-Induced Skin Oxidative Stress

    NAKAMURA Yoshimasa, OSAWA Toshihiko

    Food science and technology research   11 ( 4 )   373 - 375   2005年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:公益社団法人 日本食品科学工学会  

    We demonstrated that a dietary supplement, Antioxidant Biofactor (AOB&reg;), a fermented grain food, inhibits phorbol ester (TPA)-induced inflammatory responses. Pretreatment of mouse skin with ethyl acetate extract of AOB (0.5mg) significantly attenuated the hydrogen peroxide level in mouse skin without inhibition of edema formation. Also, exposure of AOB extract to differentiated HL-60 cells resulted in dose-dependent inhibition of TPA-induced superoxide generation, which was stronger than those of the raw materials of AOB. Our results suggest that AOB is effective for prevention of skin oxidative stress.

    DOI: 10.3136/fstr.11.373

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  • α-Tocopherol-mediated caspase-3 up-regulation enhances susceptibility to apoptotic stimuli

    Noriyuki Miyoshi, Noriyuki Miyoshi, Kisa Naniwa, Takeshi Kumagai, Takeshi Kumagai, Koji Uchida, Toshihiko Osawa, Yoshimasa Nakamura, Yoshimasa Nakamura

    Biochemical and Biophysical Research Communications   334 ( 2 )   466 - 473   2005年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Although α-tocopherol is known as an essential micronutrient involved in various oxidative stress-related processes, its non-antioxidant activities have only been characterized in recent years. In this study, we reveal that (+)-α-tocopherol [RRR-α-tocopherol] enhances cellular susceptibility to both oxidative and non-oxidative apoptosis-inducin g stimuli through up-regulation of caspase-3/CPP32 expression in several human cell lines. Exposure of (+)-α-tocopherol pretreated cells to known apoptosis-inducing stimuli, such as Fas, H 2 O 2 , or etoposide, resulted in an increase in cellular apoptotsis. In addition, (+)-α-tocopherol also elevated the pro-caspase-3 protein level and mRNA expression in a time- and dose-dependent manner, while other tocopherol analogues showed no effect. Experiments using a GC-specific DNA binding agent, mithramycin A, and an electrophoretic mobility shift assay demonstrated that Sp1 might mediate the enhanced expression of caspase-3. Our results also confirmed that (+)-α-tocopherol promotes the expression, but not the activation, of caspase-3 in various human cell lines. These findings provide biological evidence showing that (+)-α-tocopherol can amplify the apoptotic response by up-regulating the expression of pro-caspase-3. © 2005 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2005.06.113

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  • Antioxidative catechol lignans converted from sesamin and sesaminol triglucoside by culturing with Aspergillus

    Y Miyake, S Fukumoto, M Okada, K Sakaida, Y Nakamura, T Osawa

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   53 ( 1 )   22 - 27   2005年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    Sesamin and sesaminol triglucoside in sesame seeds are major lignans that display an abundance of biological activities. Although their antioxidative activity in vitro is weak, they have been reported to suppress oxidative stress in vivo. We investigated the production of new antioxidative lignans from sesame lignans by culturing with the genus Aspergillus to enhance the function of food materials. Media containing sesamin or sesaminol triglucoside increased antioxidative activity for DPPH radical scavenging by culturing with Aspergillus usamii mut. shirousamii RIB2503. The antioxidative lignans in sesamin medium were identified as sesamin 2,6-dicatechol and episesamin 2,6-dicatechol. Those in sesaminol triglucoside medium were identified as sesaminol 6-catechol and episesaminol 6-catechol, which are novel antioxidative lignans. It is suggested that they may exhibit higher antioxidative activity than sesamin and sesaminol triglucoside because they have the catechol functional moiety.

    DOI: 10.1021/jf048043h

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  • Galanals A & B, cytotoxic constituents isolated from Zingiber mioga Roscoe, interfere with the respiratory chain of liver mitchondra 査読

    K Kawai, Y Ozawa, Y Nakamura

    YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN   125   86 - 87   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PHARMACEUTICAL SOC JAPAN  

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  • Endogenous formation of novel halogenated 2′-deoxycytidine: Hypohalous acid-mediated DNA modification at the site of inflammation

    Yoshichika Kawai, Yoshichika Kawai, Hiroshi Morinaga, Hajime Kondo, Noriyuki Miyoshi, Yoshimasa Nakamura, Koji Uchida, Toshihiko Osawa

    Journal of Biological Chemistry   279 ( 49 )   51241 - 51249   2004年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A potential role of DNA damage by leukocyte-derived reactive species in carcinogenesis has been suggested. Leukocyte-derived peroxidases, such as myeloperoxidase and eosinophil peroxidase, use hydrogen peroxide and halides (Cl - and Br - ) to generate hypohalous acids (HOCl and HOBr), halogenating intermediates. It has been suggested that these oxidants lead to the formation of halogenated products upon reaction with nucleobases. To verify the consequences of phagocyte-mediated DNA damage at the site of inflammation, we developed a novel monoclonal antibody (mAb2D3) that recognizes the hypohalous acid-modified DNA and found that the antibody most significantly recognized HOCl/HOBr-modified 2′-deoxycytidine residues. The immunoreactivity of HOCl-treated oligonucleotide was attenuated by excess methionine, suggesting that chloramine-like species may be the plausible epitopes of the antibody. On the basis of further characterization combined with mass spectrometric analysis, the epitopes of mAb2D3 were determined to be novel N 4 ,5-dihalogenated 2′-deoxycytidine residues. The formation of the dihalogenated 2′-deoxycytidine in vivo was immunohistochemically demonstrated in the lung and liver nuclei of mice treated with lipopolysaccharides, an experimental inflammatory model. These results strongly suggest that phagocyte-derived oxidants, hypohalous acids, endogenously generate the halogenated DNA bases such as a novel dihalogenated 2′-deoxycytidine in vivo. Halogenation (chlorination and/or bromination) of DNA therefore may constitute one mechanism for oxidative DNA damage at the site of inflammation.

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  • Zerumbone, a tropical ginger sesquiterpene, activates phase II drug metabolizing enzymes

    Y Nakamura, C Yoshida, A Murakami, H Ohigashi, T Osawa, K Uchida

    FEBS LETTERS   572 ( 1-3 )   245 - 250   2004年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Zerumbone (ZER), a sesquiterpene compound occurring in tropical ginger Zingiber zerumbet Smith, has been implicated as one of the promising chemopreventive agents against colon and skin cancer. In the present study, we investigated the phase II detoxification enzymes induction of ZER using a cultured rat normal liver epithelial cell line. Exposure of RL34 cells to ZER resulted in the significant induction of glutathione S-transferase, while the reduced analogues of ZER (alpha-humulene and 8-hydroxy-alpha-humulene) did not show any inducing effect. Therefore, the electrophilic property, characterized by the reactivity with intracellular nucleophiles including protein sulfhydryls as well as low molecular weight thiols, at the 8-position alpha, beta-unsaturated carbonyl group plays an important role in the induction of phase II enzymes. ZER induced nuclear localization of the transcription factor Nrf2 that binds to antioxidant response element (ARE) of the phase II enzyme genes, suggesting that ZER is a potential activator of the Nrf2/ARE-dependent detoxification pathway. This is consistent with the observation that ZER potentiated the gene expression of several Nrf2/ARE-dependent phase II enzyme genes, including gamma-glutamylcysteine synthetase, glutathione peroxidase, and hemeoxygenase-1. The present study also implied the antioxidant role of this detoxification system activation by ZER in the neutralization of lipid peroxidation in hepatocytes, providing a new insight for cancer prevention. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

    DOI: 10.1016/j.febslet.2004.07.042

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  • Zerumbone, a sesquiterpene in subtropical ginger, suppresses skin tumor initiation and promotion stages in ICR mice

    A Murakami, T Tanaka, JY Lee, YJ Surh, HW Kim, K Kawabata, Y Nakamura, S Jiwajinda, H Ohigashi

    INTERNATIONAL JOURNAL OF CANCER   110 ( 4 )   481 - 490   2004年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    We recently showed that zerumbone, a sesquiterpene found in subtropical ginger, suppresses colonic tumor marker formation in rats and induces apoptosis in colon cancer cell lines. In our present study, the anti-tumor initiating and promoting activities of zerumbone in mouse skin were evaluated using a conventional 2-stage carcinogenesis model. A single topical pretreatment to mouse skin (2 mumol) 24 hr before application of dimethylbenz[a]anthracene (0.2 mumol) markedly suppressed tumor incidence by 60% and the number of tumors by 80% per mouse. Repeated pretreatment (16 nmol) twice weekly during the post-initiation phase reduced the number of 12-O-tetradecanoylphorbol-13-acetate (TPA, 1.6 nmol)-induced tumors by 83% as well as their diameter by 57%. Multiple reverse transcriptase (RT) PCR experiments revealed that zerumbone (2 mumol) enhanced the mRNA expression level of manganese superoxide dismutase, glutathione peroxidase-1, glutathione S-transferase-P1 and NAD(P)H quinone oxidoreductase in the epidermis, but not that of cytochrome P450 1A1 or 1B1. Further, it diminished TPA-induced cyclooxygenase-2 protein expression and phosphorylation of extracellular signal-regulated kinase 1/2, while pretreatment(s), in either the priming or activation stage or both, reduced double TPA application-induced hydrogen peroxide formation and edema induction by 29% to 86%, respectively. Histologic examination revealed that pretreatment(s) with zerumbone suppressed leukocyte infiltration and reduced proliferating cell nuclear antigen-labeling indices. Together, our results indicate that zerumbone is a promising agent for the prevention of both tumor initiating and promoting processes, through induction of anti-oxidative and phase II drug metabolizing enzymes as well as attenuation of proinflammatory signaling pathways. (C) 2004 Wiley-Liss, Inc.

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  • Antimicrobial activities of diterpene dialdehydes, constituents from myoga (Zingiber mioga Roscoe), and their quantitative analysis

    M Abe, Y Ozawa, Y Uda, F Yamada, Y Morimitsu, Y Nakamura, T Osawa

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   68 ( 7 )   1601 - 1604   2004年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    The antimicrobial activities of the three diterpene dialdehydes, miogadial, galanal A and galanal B, isolated from flower buds of the myoga (Zingiber mioga Roscoe) plant were investigated with some strains of bacteria, yeasts and molds. Among the three compounds, miogadial exhibited relatively greater antimicrobial activity than the others against Gram-positive bacteria and yeasts. Galanals A and B also behaved as antimicrobial agents against Gram-positive bacteria and yeasts. The content of miogadial in the flower buds was much higher than that in the leaves, whereas galanals A and B were contained at high levels in the leaves and rhizomes.

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  • Benzyl isothiocyanate inhibits excessive superoxide generation in inflammatory leukocytes: implication for prevention against inflammation-related carcinogenesis

    N Miyoshi, S Takabayashi, T Osawa, Y Nakamura

    CARCINOGENESIS   25 ( 4 )   567 - 575   2004年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Inhibitors of excessive superoxide (O-2(-)) generation have been indicated to be more effective antioxidants than radical scavengers because O-2(-) anion is one of the precursors of several types of reactive oxygen species (ROS). We demonstrated here that benzyl isothiocyanate (BITC) is a potent inhibitor of leukocytic NADPH oxidase generating a great amount of O-2(-) in oxidative burst. The exposure of BITC to the differentiated HL-60 cells resulted in the inhibition of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced O-2(-) generation, while the methylthiocarbamate analog of BITC, hardly reactive with thiols including glutathione and protein sulfhydryl, did not show any effect. Pre-treatment of the cells with diethyl maleate significantly potentiated the BITC-induced inhibition, while pre-treatment with N-acetyl-cysteine counteracted it. These results led us to assume that a plausible intracellular target molecule(s) having a reactive sulfhydryl moiety might be regulated by the covalent attachment with BITC. In spite of no ability to affect the translocation of protein kinase C beta to the membrane, BITC probably modifies the electron transport system of cytochrome b(558), consistent with the observation that BITC inhibited the substrate utilization. Pre-treatments of mouse skin with BITC significantly attenuated the TPA-enhanced hydrogen peroxide level, suggesting that BITC indeed acts as an inhibitor of O-2(-) generation in mouse skin. A histological study also demonstrated that BITC inhibited TPA-induced leukocyte infiltration in the dermis. Because we have found several O-2(-) generation inhibitors to be effective chemopreventors against mouse skin carcinogenesis, the modifying effect of the topical application of BITC on TPA-induced mouse skin tumor promotion was investigated. We demonstrated for the first time that the pre-treatment with BITC 40 min prior to each TPA treatment significantly inhibited the number of papillomas per mouse. In conclusion, the results from this study provided biological evidence that BITC has a potential to prevent inflammation-related carcinogenesis including skin cancer.

    DOI: 10.1093/carcin/bgh051

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  • Extract of vinegar "Kurosu" from unpolished rice inhibits the proliferation of human cancer cells

    K Nanda, N Miyoshi, Y Nakamura, Y Shimoji, Y Tamura, Y Nishikawa, K Uenakai, H Kohno, T Tanaka

    JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH   23 ( 1 )   69 - 75   2004年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:APSIT ASSOC PROM STUD IMMUNOL TUMOR  

    The effects of the ethyl acetate extract of "Kurosu" (EK), Japanese traditional vinegar from unpolished rice, on the proliferation of a variety of human cancer cell lines were investigated by using the alamar blue assay. Cancer cell lines included colon adenocarcinoma (Caco-2), lung carcinoma (A549), breast adenocarcinoma (MCF-7), bladder carcinoma (5637), and prostate carcinoma (LNCaP) cells. EK inhibited the proliferation of all tested cell lines in a dose-dependent manner, with inhibition mostly pronounced in Caco-2 cells (up to 62% inhibition at a dose level of 0.025%). Flow cytometry of EK-treated Caco-2 cells showed a decrease in cell number in the G(2)/M phase and an increase in the sub-G(1) phase (apoptotic). In addition, DNA fragmentation was detected in Caco-2 cells cultured with EK by immunostaining. RT-PCR analysis revealed p21 mRNA expression was induced in EK-treated Caco-2 cells. Moreover, PARP cleavage was promoted in EK-treated Caco-2 cells. These results Suggest that EK causes G(0)/G(1) arrest through p21 induction and, thus, is a potential apoptosis inducer in Caco-2 cells.

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  • A link between benzyl isothiocyanate-induced cell cycle arrest and apoptosis: Involvement of mitogen-activated protein kinases in the Bcl-2 phosphorylation

    N Miyoshi, K Uchida, T Osawa, Y Nakamura

    CANCER RESEARCH   64 ( 6 )   2134 - 2142   2004年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC CANCER RESEARCH  

    In the present study, we clarified the molecular mechanism underlying the relationship between benzyl isothiocyanate (BITC)-induced cell cycle arrest and apoptosis and the involvement of mitogen-activated protein kinases (MAPKs). The exposure of Jurkat human T-cell leukemia cells to BITC resulted in the inhibition of the G(2)-M progression that coincided with the apoptosis induction. The experiment using the phase-specific synchronized cells demonstrated that the G(2)-M phase-arrested cells are more sensitive to undergoing apoptotic stimulation by BITC than the cells in other phases. We also confirmed that BITC activated c-Jun N-terminal kinase (JNK) and p38 MAPK, but not extracellular signal-regulated kinase, at the concentration required for apoptosis induction. An experiment using a JNK-specific inhibitor SP600125 or a p38 MAPK inhibitor SB202190 indicated that BITC-induced apoptosis might be regulated by the activation of these two kinases. Conversely, BITC is likely to confine the Jurkat cells in the G(2)-M phase mainly through the p38 MAPK pathway because only the p38 MAPK inhibitor significantly attenuated the accumulation of inactive phosphorylated Cdc2 protein and the G(2)-M- arrested cell numbers. We reported here for the first time that the antiapoptotic Bcl-2 protein was phosphorylated by the BITC treatment without significant alteration of the Bcl-2 total protein amount. This was abrogated by a JNK specific inhibitor SP600125 at the concentration required for specific inhibition of the c-Jun phosphorylation. Moreover, the spontaneous phosphorylation of antiapoptotic Bcl-2 in the G(2)-M synchronized cells was enhanced synergistically by the BITC treatment. Involvement of the MAPK activation in the Bcl-2 phosphorylation and apoptosis induction also was observed in HL-60 and HeLa cells. Thus, we identified the phosphorylated Bcl-2 as a key molecule linking the p38 MAPK-dependent cell cycle arrest with the JNK activation by BITC.

    DOI: 10.1158/0008-5472.CAN-03-2296

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  • Benzyl isothiocyanate modifies expression of the G(2)/M arrest-related genes

    N Miyoshi, K Uchida, T Osawa, Y Nakamura

    BIOFACTORS   21 ( 1-4 )   23 - 26   2004年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IOS PRESS  

    Naturally occurring isothiocyanates are effective chemoprotective agents against chemical carcinogenesis in experimental animals. In the present study, we clarified the molecular mechanism underlying the relationship between benzyl isothiocyanate (BITC)-induced cell cycle arrest and apoptosis. The exposure of HL-60 cells to BITC resulted in the inhibition of the G(2)/M progression that coincided with the apoptosis induction. We demonstrated that BITC significantly up-regulated expression of the G(2)/M cell cycle arrest-regulating genes including p21, GADD45, and 14-3-3sigma. Thus, these gathered data further supported that BITC has a potential to induce apoptosis selectively in the proliferating pre-cancerous cells through a cell cycle arrest-dependent mechanism.

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  • Benzyl isothiocyanate inhibits oxidative stress in mouse skin: Involvement of attenuation of leukocyte infiltration

    Y Nakamura, N Miyoshi, S Takabayashi, T Osawa

    BIOFACTORS   21 ( 1-4 )   255 - 257   2004年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:IOS PRESS  

    The exposure of benzyl isothiocyanate (BITC) to mouse skin resulted in the attenuation of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced oxidative damage through not only inhibition of the NADPH oxidase system but also leukocyte clearance at inflamed region. In spite of little ability to affect TPA-induced edema formation, pretreatments of mouse skin with BITC before the first or second TPA treatment significantly decrease the H2O2 level. A histological study also demonstrated that BITC enhanced the terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL)-positive index in mouse skin, suggesting that BITC might accelerate the disappearance of infiltrated leukocytes. Thus, these gathered data further supported that BITC has a potential as an anti-inflammatory agent.

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  • Arachidonic acid cascade inhibitors modulate phorbol ester-induced oxidative stress in female ICR mouse skin: Differential roles of 5-lipoxygenase and cyclooxygenase-2 in leukocyte infiltration and activation

    Y Nakamura, M Kozuka, K Naniwa, S Takabayashi, K Torikai, R Hayashi, T Sato, H Ohigashi, T Osawa

    FREE RADICAL BIOLOGY AND MEDICINE   35 ( 9 )   997 - 1007   2003年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    We recently developed a short-term assay for skin H2O2 generation induced by double 12-O-tetradecanoylphorbol-13-acetate (TPA) applications for mechanistic study on skin epidermal carcinogenesis. In the present study, we investigated the individual roles of arachidonic acid metabolism in H2O2 generation in mouse skin inflammation. The experiments using inhibitors of arachidonic acid (AA) metabolism showed that corticosteroid and a lipoxygenase (LO) inhibitor expectedly suppressed double TPA application-induced H2O2 generation through the interference of chemotactic action but not by direct decomposition or scavenging. We also demonstrated that the treatment of AA (1 mumol) and 5-LO metabolites including leukotriene B-4 (LTB4) partly mimicked, though soybean LO-derived lipid hydroperoxide and prostaglandins did not, the priming effect evaluated by edema formation and leukocyte infiltration. We also confirmed that inflammatory leukocytes accumulated by LTB4 generated a significant amount of H2O2 by TPA stimulation. These results suggested that 5-LO metabolites of AA are the potential key molecules in the TPA-induced priming event. Interestingly, the cyclooxygenase (COX-) 2-selective inhibitor nimesulide (NS) and celecoxib (CXB) showed different responses than those of other inhibitors. These agents showed no specific potential to inhibit the priming event but significantly suppressed H2O2 generation, lipid peroxidation, and hyperplasia in mouse skin. From the results based on an in vitro leukocyte differentiation model, we speculated that the antioxidant effect of the COX-2 inhibitors might be partly associated with both counteraction of proinflammatory cytokine-enhanced ROS generation and inhibition of CD11b, an important molecule for cell adhesion, expression. Indeed, the topical application of NS attenuated the number of infiltrated leukocytes induced by TPA in mouse skin. Thus, these gathered data indicated the differential roles of 5-LO and COX-2 in leukocyte adhesion, infiltration, and H2O2 generation. (C) 2003 Elsevier Inc.

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  • Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells

    N Miyoshi, Y Nakamura, Y Ueda, M Abe, Y Ozawa, K Uchida, T Osawa

    CANCER LETTERS   199 ( 2 )   113 - 119   2003年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    The effects of the constituents isolated from ginger species including curcumin, 6-gingerol and labdane-type diterpene compounds on cell proliferation and the induction of apoptosis in the cultured human T lymphoma Jurkat cells were studied. Among the tested compounds, Galanals A and 13, isolated from the flower buds of a Japanese ginger, myoga (Zingiber mioga Roscoe), showed the most potent cytotoxic effect. Exposure Of Jurkat human T-cell leukemia cells to galanals resulted in the induction of apoptotic cell death characterized by DNA fragmentation and caspase-3 activation. The mitochondrial damage pathway was suggested to be involved in galanal-induced apoptosis because the treatment of cells with galanals induced mitochondrial transmembrane potential (Deltapsim) alteration and cytochrome c release. The anti-apoptotic Bcl-2 protein was downregulated by the galanal treatment together with enhancement of the Bax expression. In conclusion, the results from this study provide biological evidence that ginger-specific constituents other than curcuminoids are potential anticancer agents. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

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  • Correlation of antimutagenic activity and suppression of CYP1A with the lipophilicity of alkyl gallates and other phenolic compounds

    Q Feng, T Kumagai, Y Nakamura, K Uchida, T Osawa

    MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS   537 ( 1 )   101 - 108   2003年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Alkyl gallates are widely used as food antioxidants. Methyl, ethyl, propyl, lauryl, and cetyl gallates showed antimuta-genicity to activated 2-aminoanthracene (2AA)-induced SOS responses in Salmonella typhimurium TA1535/pSK1002. They also exhibited a suppressive effect on 3-methylcholanthrene (3-MC)-induced cytochrome P450 1A (CYP1A) in human hepatoma HepG2 cells, as indexed by the 7-ethoxyresorufin-O-deethylase (EROD) activity, and on CYP1A protein level. Both antimutagenicity and suppression of CYP1A appeared to be dependent on alkyl chain lengths, which suggested lipophilicity dependence. Based on those results, we investigated 26 other phenolic compounds for their lipophilicity, antimutagenicity and inhibition of EROD activity. The lipophilicity correlated well with the inhibition of EROD activity (r = 0.78), and the inhibition of EROD activity correlated with the antimutagenicity of those compounds (r = 0.71). The results suggest that the lipophilicity of the phenolic compounds may be an important factor in their ability to inhibit EROD activity. (C) 2003 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S1383-5718(03)00057-3

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  • Dihydrochalcones: Evaluation as novel radical scavenging antioxidants

    Y Nakamura, S Watanabe, N Miyake, H Kohno, T Osawa

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   51 ( 11 )   3309 - 3312   2003年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    Dihydrochalcones are a family of bicyclic flavonoids, defined by the presence of two benzene rings joined by a saturated three carbon bridge. In the present study, we systematically examined the antioxidant activities of dihydrochalcones against the stable free radical (1,1-diphenyl-2-picrylhydrazyl) and lipid peroxidation in the erythrocyte membrane. All dihydrochalcones exhibited higher antioxidant activities than the corresponding flavanones. The H-1 NMR analysis indicated that the active dihydrochalcone has a time-averaged conformation in which the aromatic A ring is orthogonal to the carbonyl group, while the inactive dihydrochalcone such as 2'-O-methyl-phloretin has a strongly hydrogen-bonded phenolic hydroxyl group, suggestive of a coplanar conformation. A hydroxyl group at the 2'-position of the dihydrochalcone A ring, newly formed by reduction of the flavanone C ring, is an essential pharmacophore for its radical scavenging potential.

    DOI: 10.1021/jf0341060

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  • Structures of (-)-epicatechin glucuronide identified from plasma and urine after oral ingestion of (-)-epicatechin: Differences between human and rat

    M Natsume, N Osakabe, M Oyama, M Sasaki, S Baba, Y Nakamura, T Osawa, J Terao

    FREE RADICAL BIOLOGY AND MEDICINE   34 ( 7 )   840 - 849   2003年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    (-)-Epicatechin is one of the most potent antioxidants present in the human diet. Particularly high levels are found in black tea, apples, and chocolate. High intake of catechins has been associated with reduced risk of cardiovascular diseases. There have been several reports concerning the bioavailability of catechins, however, the chemical structure of (-)-epicatechin metabolites in blood, tissues, and urine remains unclear. In the present study, we purified and elucidated the chemical structure of (-)-epicatechin metabolites in human and rat urine after oral administration. Three metabolites were purified from human urine including (-)-epicatechin-3'-O-glucuronide, 4'-O-methyl-(-)-epicatechin-3'-O-glucuronide, and 4'-O-methyl-(-)-epicatechin-5 or 7-O-glucuronide, according to H-1- and C-13-NMR, HMBC, and LC-MS analyses. The metabolites purified from rat urine were 3'-O-methyl-(-)-epicatechin, (-)-epicatechin-7-O-glucuronide, and 3'-O-methyl-(-)-epicatechin-7-O-glucuronide. These compounds were also detected in the blood of humans and rats by LC-MS. The presence of these metabolites in blood and urine suggests that catechins are metabolized and circulated in the body after administration of catechin-containing foods. (C) 2003 Elsevier Science Inc.

    DOI: 10.1016/S0891-5849(02)01434-X

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  • Pivotal role of electrophilicity in glutathione S-transferase induction by tert-butylhydroquinone

    Y Nakamura, T Kumagai, C Yoshida, Y Naito, M Miyamoto, H Ohigashi, T Osawa, K Uchida

    BIOCHEMISTRY   42 ( 14 )   4300 - 4309   2003年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    Although the induction of glutathione S-transferase (GST) activity by tert-butylhydroquinone (tBHQ) has been well-documented in several cell culture systems and rodent experiments, the exact mechanism responsible for its inducibility is still not thoroughly understood. To more precisely define the molecular mechanism of GST induction by tBHQ, we examined the one-electron oxidation and glutathione (GSH) reaction potentials of tBHQ as compared to its analogue, 2,5-di-tert-butylhydroquinone (DtBHQ). tBHQ and DtBHQ showed similar one-electron oxidation potentials, including free radical quenching (antioxidant), oxidative conversion of both compounds to a benzoquinone form, and Cu2+-dependent superoxide generation. On the other hand, the reduced GSH level was observed by the addition of tBHQ, but not DtBHQ, suggesting that tBHQ acts as an electrophile while DtBHQ does not. The data were consistent with the observation that tBHQ more potently induced the GSTP1 gene expression in RL34 cells than DtBHQ did. Moreover, we indeed detected the GSH-tBHQ conjugates in the cells exposed to tBHQ using an electrochemical detector-high-performance liquid chromatography technique. Thus, we conclude that an electrophilic quinone oxidation product that reacts with intracellular nucleophiles including protein thiol or GSH plays a major role in the GSTP1 gene expression.

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  • A phase II detoxification enzyme inducer from lemongrass: identification of citral and involvement of electrophilic reaction in the enzyme induction

    Y Nakamura, M Miyamoto, A Murakami, H Ohigashi, T Osawa, K Uchida

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   302 ( 3 )   593 - 600   2003年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    We have developed a simple system for the sensitive detection and measurement of glutathione S-transferase (GST) activity that detoxifies polycyclic aromatic hydrocarbons using the cultured rat normal liver epithelial cell line, RL34 cells. Citral (3,7-dimethyl-2,6-octadienal) was isolated from the methanol extract of lemongrass (Cymbopogon citratus) and identified as a novel inducer of GST. Citral, a mixture of the two stereoisomers geranial and neral, dose- and time-dependently induced the total and pi-class-specific activities of GST. The structure-activity relationship study revealed that geranial, an E-isomer, was mainly responsible for the inducing activity of citral mixture and the aldehyde group conjugated with a trans-double bond is an essential structural factor. The data were consistent with the in vitro observation that both glutathione (GSH) and protein thiol quickly and specifically reacted with the active isomer geranial, but not neral. Pretreatment of the cells with diethyl maleate significantly enhanced not only the basal activity but also the citral-stimulated activity of GST, while pretreatment with N-acetyl-cysteine inhibited it. Moreover, the treatment of RL 34 cells with geranial for 30 min significantly attenuated the intracellular GSH level, while application for 18 h enhanced it. These results strongly suggested that the electrophilic property characterized by the reactivity with intracellular nucleophiles including protein thiol or glutathione (GSH) plays an important role in the induction of GST. The present study also implied the antioxidant role of GST induction by citral in mouse skin, providing a new insight into skin cancer prevention. (C) 2003 Elsevier Science (USA). All rights reserved.

    DOI: 10.1016/S0006-291X(03)00219-5

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  • Induction of cytochrome P4501Al by autoclavable culture medium change in HepG2 cells 査読

    Q Feng, T Kumagai, Y Nakamura, K Uchida, T Osawa

    XENOBIOTICA   32 ( 11 )   1033 - 1043   2002年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    1. Without the addition of xenobiotics, only by changing the Culture medium can one induce extensively and transiently cytochrome P4501A1 (CYP1A1) protein and mRNA in human hepatoma HepG2 cells. The induction was aryl hydrocarbon receptor (AhR)dependent, and was proven by: (1) the medium change activated the AhR, as judged by a electrophoretic mobility shift assay; and (2) the AhR inhibitor alpha-naphthoflavone inhibited the medium change-mediated induction.
    2. Induction of CYP1A1 was related to medium prepared by autoclaving. By screening the ingredients in the medium, the serum had no effect on CYP1A1 induction, whereas both photo-oxidized and autoclaved tryptophan were shown to induce CYP1A1, as indicated by CYP1A1 protein or ethoxyresorufin-O-deethylase activity. The autoclaved tryptophan contained in an autoclavable medium was a more potent inducer of CYP1A1 than photo-oxidized tryptophan.
    3. The results provide some practical suggestions with experiments related to CYP1A1.

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  • Isolation and identification of DPPH radical scavenging compounds in Kurosu (Japanese unpolished rice vinegar)

    Y Shimoji, Y Tamura, Y Nakamura, K Nanda, S Nishidai, Y Nishikawa, N Ishihara, K Uenakai, H Ohigashi

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   50 ( 22 )   6501 - 6503   2002年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    Dihydroferulic acid (DFA) and dihydrosinapic acid (DSA) were isolated from Kurosu (unpolished rice vinegar) as the major constituents responsible for Kurosu's radical scavenging activity. The levels of antioxidative activity of DFA and DSA in DPPH radical scavenging were higher than those of their respective structurally related compounds, ferulic acid and sinapic acid. The concentrations of DFA and DSA were low in common rice vinegar (polished rice vinegar), suggesting that Kurosu is more advantageous than rice vinegars as an antioxidative food item. As the concentrations of DFA and DSA were low in unpolished rice, too, these acids are thought to be produced in Kurosu through the process of the fermentation from ferulic acid and sinapic acid, respectively.

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  • FA15, a hydrophobic derivative of ferulic acid, suppresses inflammatory responses and skin tumor promotion: comparison with ferulic acid

    A Murakami, Y Nakamura, K Koshimizu, D Takahashi, K Matsumoto, K Hagihara, H Taniguchi, E Nomura, A Hosoda, T Tsuno, Y Maruta, HW Kim, K Kawabata, H Ohigashi

    CANCER LETTERS   180 ( 2 )   121 - 129   2002年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    In our previous study. FA15 (2-methyl-I-butyl ferulic acid) was chemically synthesized as a novel ferulic acid (FA) analog, and found to notably suppress phorbol ester-induced Epstein-Barr virus activation and superoxide anion generation in vitro. In this report, we demonstrated that FA15 in contrast to FA, markedly Suppressed the combined lipopolysaccharide and interferon-gamma-induced protein expressions of inducible nitric oxide synthase and cyclooxygenase-2, and also inhibited the release of tumor necrosis factor-alpha accompanied by suppression of I-kappaB degradation in RAW264.7, a murine macrophage cell line. In ICR mouse skin, topical application of FA15 significantly attenuated phorbol ester-triggered hydrogen peroxide production and edema formation as well as papilloma development while that of FA did not. Our results suggest that FA15, derived front natural sources. is a novel chemopreventive agent, both structurally and functionally. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

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  • Zerumbone, a Southeast Asian ginger sesquiterpene, markedly suppresses free radical generation, proinflammatory protein production, and cancer cell proliferation accompanied by apoptosis: The α,β-unsaturated carbonyl group is a prerequisite

    Akira Murakami, Daisuke Takahashi, Takashi Kinoshita, Koichi Koshimizu, Ha Won Kim, Akira Yoshihiro, Yoshimasa Nakamura, Suratwadee Jiwajinda, Junji Terao, Hajime Ohigashi

    Carcinogenesis   23 ( 5 )   795 - 802   2002年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Zerumbone (ZER), a sesquiterpene from the edible plant Zingiber zerumbet Smith, has recently been found to suppress tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced Epstein-Barr virus activation in a potent manner. In the present study, we evaluated the anti-inflammatory and chemopreventive potentials of ZER in a variety of cell culture experiments. ZER effectively suppressed TPA-induced superoxide anion generation from both NADPH oxidase in dimethylsulfoxide-differentiated HL-60 human acute promyelocytic leukemia cells and xanthine oxidase in AS52 Chinese hamster ovary cells. The combined lipopolysaccharide- and interferon-γ-stimulated protein expressions of inducible nitric oxide synthase and cyclooxygenase (COX)-2, together with the release of tumor necrosis factor-α, in RAW 264.7 mouse macrophages were also markedly diminished. These suppressive events were accompanied with a combined decrease in the medium concentrations of nitrite and prostaglandin E 2 , while the expression level of COX-1 was unchanged. ZER inhibited the proliferation of human colonic adenocarcinoma cell lines (LS174T, LS180, COLO205, and COLO320DM) in a dose-dependent manner, while the growth of normal human dermal (2F0-C25) and colon (CCD-18 Co) fibroblasts was less affected. It also induced apoptosis in COLO205 cells, as detected by dysfunction of the mitochondria transmembrane, Annexin V-detected translocation of phosphatidylserine, and chromatin condensation. Intriguingly, α-humulene, a structural analog lacking only the carbonyl group in ZER, was virtually inactive in all experiments conducted, indicating that the α,β-unsaturated carbonyl group in ZER may play some pivotal roles in interactions with unidentified target molecule(s). Taken together, our results indicate that ZER is a food phytochemical that has distinct potentials for use in anti-inflammation, chemoprevention, and chemotherapy strategies.

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  • Involvement of the mitochondrial death pathway in chemopreventive benzyl isothiocyanate-induced apoptosis

    Y Nakamura, M Kawakami, A Yoshihiro, N Miyoshi, H Ohigashi, K Kawai, T Osawa, K Uchida

    JOURNAL OF BIOLOGICAL CHEMISTRY   277 ( 10 )   8492 - 8499   2002年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    In the present study, we studied the molecular mechanism underlying cell death induced by a cancer chemoprotective compound benzyl isothiocyanate (BITC). The cytotoxic effect of BITC was examined in rat liver epithelial RL34 cells. Apoptosis was induced when the cells were treated with 20 muM BITC, characterized by the appearance of phosphatidylserine on the outer surface of the plasma membrane and caspase-3 activation, whereas no caspase activation and propidium iodide incorporation into cell were detected with 50 muM BITC that induced necrosis. The mitochondrial death pathway was suggested to be involved in BITC-induced apoptosis because the treatment of cells with BITC-induced caspase-9-dependent apoptosis and mitochondrial transmembrane potential (DeltaPsim) alteration. We demonstrated here for the first time that BITC directly modifies mitochondrial functions, including inhibition of respiration, mitochondrial swelling, and release of cytochrome c. Moreover, glutathione depletion by diethyl maleate significantly accelerated BITC-triggered apoptosis, suggesting the involvement of a redox-dependent mechanism. This was also implicated by the observations that intracellular accumulation of reactive oxygen species, including superoxide (O-2(.-)) and hydroperoxides (HPOs), was indeed detected in the cells treated with BITC and that the intracellular HPO level was significantly attenuated by pretreatment with N-ace-tylcysteine. The treatment with a pharmacological scavenger of 07 Tiron, also diminished the HPO formation by similar to80%,c, suggesting that most of the HPOs were H2O2 derived from the dismutation of O-2(.-). These results suggest that BITC induces apoptosis through a mitochondial redox-sensitive mechanism.

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  • Screening of edible Japanese plants for suppressive effects on phorbol ester-induced superoxide generation in differentiated HL-60 cells and AS52 cells

    HW Kim, A Murakami, Y Nakamura, H Ohigashi

    CANCER LETTERS   176 ( 1 )   7 - 16   2002年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    Epithelial xanthine oxidase (XOD) is one of the major enzymes responsible for superoxide (O-2(-)) generation, which is involved in oxidative stress. However, there are few known reports of a convenient bioassay to detect Cellular XOD activity. We tested several cell lines, and found that AS52, from Chinese hamster ovary cells, produced a significant level of O-2(-) in 2 response to 12-O-tetradecanoylphorbol 13-acetate (TPA), and this activity was markedly inhibited by allopurinol, an XOD inhibitor. Using AS52 cells and differentiated HL-60 cells, we conducted screening tests of edible Japanese plant extracts for their inhibitory activities toward TPA-induced O-2(-) generation from both reduced nicotinamide adenine dinucleotide oxidase (HL-60) and XOD (AS52). Notably, the extracts from mioga ginger, rape, avocado, carrot, turnip, taro, and shiineji showed potent inhibition of O-2(-) generation in both cell lines. These results suggest that several edible Japanese plants carry a significant antioxidative and cancer preventive potential. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

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  • A sulforaphane analogue that potently activates the Nrf2-dependent detoxification pathway

    Y Morimitsu, Y Nakagawa, K Hayashi, H Fujii, T Kumagai, Y Nakamura, T Osawa, F Horio, K Itoh, K Iida, M Yamamoto, K Uchida

    JOURNAL OF BIOLOGICAL CHEMISTRY   277 ( 5 )   3456 - 3463   2002年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Exposure of cells to a wide variety of chemoprotective compounds confers resistance to a broad set of carcinogens. For a subset of the chemoprotective compounds, protection is generated by an increase in the abundance of the protective phase II detoxification enzymes, such as glutathione S-transferase (GST). We have recently developed a cell culture system, using rat liver epithelial RL 34 cells, that potently responds to the phenolic antioxidants resulting in the induction of GST activity (Kawamoto, Y., Nakamura, Y., Naito, Y., Torii, Y., Kumagai, T., Osawa, T., Ohigashi, H., Satoh, K., Imagawa, M., and Uchida, K. (2000) J. Biol. Chem. 275, 11291-11299.) In the present study, we investigated the phase II-inducing potency of an isothiocyanate compound in vitro and in vivo and examined a possible induction mechanism. Based on an extensive screening of vegetable extracts for GST inducer activity in RL34 cells, we found Japanese horseradish, wasabi (Wasabia japonica, syn. Eutrema wasabi), as the richest source and identified 6-methylsulfinylhexyl isothiocyanate (6-HITC), an analogue of sulforaphane (4-methylsulfinylbutyl isothiocyanate) isolated from broccoli, as the major GST inducer in wasabi. 6-HITC potently induced both class alpha GSTA1 and class pi GSTP1 isozymes in RL34 cells. In animal experiments, we found that 6-MSHI was rapidly absorbed into the body and induced hepatic phase 11 detoxification enzymes more potently than sulforaphane. The observations that W 6-HITC activated the antioxidant response element (ARE), (ii) 6-HITC induced nuclear localization of the transcription factor Nrf2 that binds to ARE, and (iii) the induction of phase 11 enzyme genes by 6-HITC was completely abrogated in the nrf2-deficient mice, suggest that 6-HITC is a potential activator of the Nrf2/ARE-dependent detoxification pathway.

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  • Labdane-type Diterpene Dialdehyde, Pungent Principle of Myoga, Zingiber mioga Roscoe

    Masako Abe, Yoshio Ozawa, Yasushi Uda, Yoichi Yamada, Yasujiro Morimitsu, Yoshimasa Nakamura, Toshihiko Osawa

    Bioscience, Biotechnology and Biochemistry   66 ( 12 )   2698 - 2700   2002年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The pungent principle of myoga (Zingiber mioga Roscoe) was identified as (E)-8β(17)-epoxylabd-12-ene-15,16-dial (miogadial) on the basis of its physical and spectroscopic properties (MS, NMR, IR, and UV). Galanal A and B, isolated as well as miogadial, had no hot taste. Reduced miogadial also was tasteless. The pungency of miogadial depended on the presence of αβ-unsaturated-1,4-dialdehyde group. © 2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.

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  • Black tea polyphenols, theaflavins, prevent cellular DNA damage by inhibiting oxidative stress and suppressing cytochrome P450 1A1 in cell cultures

    Q Feng, Y Torii, K Uchida, Y Nakamura, Y Hara, T Osawa

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   50 ( 1 )   213 - 220   2002年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    Tea polyphenols have been demonstrated as chemopreventive agents in a number of experimental models. However, less is known about the mechanism of chemoprevention by black tea compared with that of green tea. Some beneficial properties of theaflavins, the black tea polyphenols, were investigated in the present study. Theaflavins showed inhibitory effects on H2O2- and tert-butyl hydroperoxide (tBuOOH)-induced cytotoxicity (evaluated by tetrazolium bromide reduction), cellular oxidative stress (detected by oxidation of 2', 7'-dichlorofluorescin), and DNA damage (measured by amount of 8-OHdG and comet assay) in rat normal liver epithelium cell RL-34 cell lines. In addition, theaflavins also exhibited suppression of cytochrome P450 1A1 induced by omeprazole in the human hepatoma HepG2 cell line. Furthermore, when HepG2 cells were pretreated with omeprazole to induce CYP1A1, then exposed to benzo[a]pyrene (B[a]P), DNA damage was observed using the comet assay. However, theaflavins could inhibit this DNA damage. These results indicated that theaflavins could prevent cellular DNA damage by inhibiting oxidative stress and suppressing cytochrome P450 1A1 in cell cultures.

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  • Role of p38 mitogen-activated protein kinase in the 4-hydroxy-2-nonenal-induced cyclooxygenase-2 expression

    T Kumagai, Y Nakamura, T Osawa, K Uchida

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   397 ( 2 )   240 - 245   2002年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    COX-2 is rapidly expressed by various stimuli and plays a key role in conversion of free arachidonic acid to prostaglandins (PGs). 4-Hydroxy-2-nonenal (HNE), one of the lipid peroxidation end-products, has been recently identified as a potent COX-2 inducer in rat epithelial cell RL34 cells (Kumagai et al (2000) Biochem. Biophys. Res. Commun. 273, 437-441. Here we investigated the molecular mechanism underlying the COX-2 induction by HNE mainly focusing on the activation of p38 mitogen-activated protein kinase (MAPK) pathways. The observations that (i) HNE induced phosphorylation of p38 MAPK and MKK3/MKK6 within 5 min and that (ii) SB203580, a p38 MAPK-specific inhibitor, suppressed the HNE-induced COX-2 expression suggested that the p38 MAPK pathway was involved in the HNE-induced COX-2 expression. Overexpression of p38 MAPK enhanced the HNE-induced COX-2 expression, whereas the overexpression of dominant negative p38 MAPK suppressed it. Furthermore, we also found that HNE upregulated the COX-2 expression by the stabilization of COX-2 mRNA via the p38 MAPK pathway. (C) 2001 Elsevier Science.

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  • Ebselen, a glutathione peroxidase mimetic seleno-organic compound, as a multifunctional antioxidant - Implication for inflammation-associated carcinogenesis

    Y Nakamura, Q Feng, T Kumagai, K Torikai, H Ohigashi, T Osawa, N Noguchi, E Niki, K Uchida

    JOURNAL OF BIOLOGICAL CHEMISTRY   277 ( 4 )   2687 - 2694   2002年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Ebselen, a seleno-organic compound showing glutathione peroxidase-like activity, is one of the promising synthetic antioxidants. In the present study, we investigated the antioxidant activities of ebselen using a 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated mouse skin model. Double pretreatments of mouse skin with ebselen significantly inhibited TPA-induced formation of thiobarbituric acid-reacting substance, known as an overall oxidative damage biomarker, in mouse epidermis, suggesting that ebselen indeed acts as an antioxidant in mouse skin. The andoxidative effect of ebselen is attributed to its selective blockade of leukocyte infiltration and activation leading to attenuation of the H2O2 level. In in vitro studies, ebselen inhibited TPA-induced superoxide generation in differentiated HL-60 cells and lipopolysaccharide-induced cyclooxygenase-2 protein expression in RAW 264.7 cells. In addition, we demonstrated for the first time that ebselen potentiated phase II enzyme activities, including NAD(P)H:(quinone-acceptor) oxidoreductasel and glutathione S-transferase in cultured hepatocytes and in mouse skin. These results strongly suggest that ebselen, a multifunctional antioxidant, is a potential chemopreventive agent in inflammation-associated carcinogenesis.

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  • Search for chemopreventive agents on the basis of the involvement of oxidative stress and inflammation in carcinogenesis 査読

    A Murakami, Y Nakamura, K Koshimizu, H Ohigashi

    ANIMAL CELL TECHNOLOGY : BASIC & APPLIED ASPECTS, VOL 12   57 - 61   2002年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:SPRINGER  

    Cancer chemoprevention with food phytochemicals is currently regarded as one of the noticeable scientific fields. We have screened more than 400 extracts from vegetables and fruits for the anti-tumor promoting activity using a cell culture system. The Zingiberaceae plants in Southeast Asian countries were indicated to be notable sources to identify effective cancer chemopreventive agents. In addition, F-acetoxychavicol acetate (ACA), a phenyl propanoid, isolated from the rhizomes of Languas galanga, was found to be a potent chemopreventive agent which markedly suppresses phorbol ester-induced superoxide (O-2(.)) generation and endotoxin-induced nitric oxide (NO) production.

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  • A catechol antioxidant protocatechuic acid potentiates inflammatory leukocyte-derived oxidative stress in mouse skin via a tyrosinase bioactivation pathway

    Y Nakamura, K Torikai, H Ohigashi

    FREE RADICAL BIOLOGY AND MEDICINE   30 ( 9 )   967 - 978   2001年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The modifying effects of topical application of a catechol antioxidant protocatechuic acid (PA) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammatory responses in mouse skin were investigated. Treatment with a high dose (20,000 nmol) of PA, based on time of application, modifies inflammatory responses in the skin of the B6C3F(1) mouse, a resistant strain to inflammatory response induction by TPA, but shows much higher tyrosinase expression than that of an albino mouse. The responsibility of a large amount of PA-induced leukocyte infiltration to an inflamed region in a B6C3F(1) mouse is mon sensitive than that of an ICR albino mouse. When ICR mice were treated with TPA (1.6 nmol) twice weekly for 5 weeks to induce chronic inflammatory responses, pretreatment with 1600 nmol PA 30 min prior to each TPA treatment significantly enhanced the inflammatory responses including edema formation, leukocyte infiltration, and the level of thiobarbituric acid-reacting substances. The dose-dependency was closely parallel to the results of a tumor promotion study of PA previously reported. Further, the treatment of PA alone resulted in tyrosinase-dependent contact hypersensitivity in ICR mouse skin. In addition, the in vitro study of cytotoxicity demonstrated that bioactivation by tyrosinase but not myeloperoxidase of PA significantly enhanced cytotoxicity and intracellular glutathione consumption. We conclude that the tyrosinase-derived reactive quinone intermediate(s) of PA, which binds nucleophilic residues of proteins including sulfhydryl group and conjugates of which are recognized as haptens, was partially involved in alteration of the cellular immune functions including oxygen radical-generating leukocytes migration to inflamed regions. (C) 2001 Elsevier Science Inc.

    DOI: 10.1016/S0891-5849(01)00481-6

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  • The amide hydrogen of (-)-indolactam-V and benzolactam-V8's plays a critical role in protein kinase C binding and tumor-promoting activities

    Y Nakagawa, K Irie, Y Nakamura, H Ohigashi

    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   11 ( 5 )   723 - 728   2001年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    To investigate the role of the amide hydrogen of (-)-indolactam-V (1) and benzolactam-V8's on protein kinase C (PKC) binding and tumor promotion, 8-decylbenzolactone-V8 (6), a new lactone analogue of 8-decylbenzolactam-V8 (4), was synthesized from 2-nitrophenylpyruvic acid (7) in 11 steps. The PKC binding ability and tumor-promoting activities in vitro of 6 were much lower than those of 1 and 4, suggesting that the amide hydrogen of 1 and benzolactam-V8's plays a critical role in tumor promotion. However, it is noteworthy that 6 showed significant selectivity in the PKC isozyme surrogate binding. (C) 2001 Published by Elsevier Science Ltd.

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  • The C4 hydroxyl group of phorbol esters is not necessary for protein kinase C binding

    M Tanaka, K Irie, Y Nakagawa, Y Nakamura, H Ohigashi, PA Wender

    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   11 ( 5 )   719 - 722   2001年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    To investigate the role of the hydroxyl group at position 4 of the phorbol esters in protein kinase C (PKC) binding and function, 4 beta -deoxy-phorbol-12,13-dibutyrate (4 beta -deoxy-PDBu, 5a) and 4 beta -deoxy-phorbol-13-acetate (6a) were synthesized from phorbol (1). The binding affinities of these 4 beta -deoxy compounds (5a, 6a) to the 13 PKC isozyme C1 domains were quite similar to those of the corresponding 4 beta -hydroxy compounds (4a, 4b), suggesting that the C4 hydroxyl group of phorbol esters is not necessary for PKC binding. Moreover, functional assays showed that 4 beta -deoxy-PDBu (5a) exhibited biological activities (Epstein-Barr virus induction and superoxide generation) equally potent to those of PDBu (4a). These solution phase results differ from expectations based on the previously reported solid-phase structure of the complex of PKC delta -C1B and phorbol-13-acetate (4b). (C) 2001 Elsevier Science Ltd. All rights reserved.

    DOI: 10.1016/S0960-894X(01)00045-2

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  • Anticarcinogenic antioxidants as inhibitors against intracellular oxidative stress

    Q Feng, T Kumagai, Y Torii, Y Nakamura, T Osawa, K Uchida

    FREE RADICAL RESEARCH   35 ( 6 )   779 - 788   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Oxidative stress has been implicated in the pathogenesis of numerous diseases, including cancer. In the present study, the protective effect of natural antioxidants, such as quercetin and tea polyphenols, on intracellular oxidative stress was studied. Here we report a novel function of quercetin and tea polyphenols, as potential inhibitors of 4-hydroxy2-nonenal (HNE)-induced intracellular oxidative stress and cytotoxicity In rat liver epithelial RL34 cells, a potent electrophile HNE dramatically induced the productions of reactive oxygen species (ROS), which correlated well with the reduction in cell viability We found that quercetin and tea polyphenols, such as epigallocatechin gallate and theaflavins and their gallate esters, significantly inhibited the HNE-induced ROS production and cytotoxicity. In addition, HNE induced a transient decrease in the mitochondrial membrane potential (Deltapsi), which was also retarded by the antioxidants. These data suggest that the antioxidants, such as quercetin and tea polyphenols, are inhibitors against mitochondrial ROS production.

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  • Toxic dose of a simple phenolic antioxidant, protocatechuic acid, attenuates the glutathione level in ICR mouse liver and kidney

    Y. Nakamura, K. Torikai, H. Ohigashi

    Journal of Agricultural and Food Chemistry   49 ( 11 )   5674 - 5678   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    It has previously been reported that a toxic dose of protocatechuic acid (PA), a naturally occurring simple phenolic antioxidant in dietary plant foodstuff, has a potential to enhance tumorigenesis and induce contact hypersensitivity in mouse skin. In this study, the modifying effect of a toxic dose of PA on the glutathione (GSH) level in mouse liver and kidney was examined. Intraperitoneal administration of PA (500 mg/kg) caused significant hepatic and nephrotic GSH depletion. Interestingly, slight but significant hepatotoxicity and nephrotoxicity, characterized by the enhancement of plasmic alanine aminotrasferase (ALT) activity and urea level, respectively, were also observed. The subchronic administration of PA (0.1% in drinking water) for 60 days showed not only a significant decrease in the GSH level in kidney but also a significant enhancement of ALT activity in plasma. The protective role of GSH for acute hepatotoxicity using GSH-depleted mice administered a GSH synthesis inhibitor buthionine sulfoximine was also demonstrated. Thus, it is suggested that overdoses of PA can disturb the detoxification of other electrophilic toxicants including ultimate carcinogens.

    DOI: 10.1021/jf0106594

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  • Suppression by citrus auraptene of phorbol ester and endotoxin-induced inflammatory responses: role of attenuation of leukocyte activation

    A Murakami, Y Nakamura, T Tanaka, K Kawabata, D Takahashi, K Koshimizu, H Ohigashi

    CARCINOGENESIS   21 ( 10 )   1843 - 1850   2000年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Auraptene (AUR), a citrus coumarin derivative, is one of the promising chemopreventive agents against skin, tongue, esophagus and colon carcinogenesis in rodents, We reported previously that AUR suppresses superoxide anion (O-2(-)) generation from inflammatory leukocytes in in vitro experiments, In the present study, we investigated the antiinflammatory activities of AUR using a 12-O-tetradecanoylphorbol-13-acetate-treated mouse skin model, and compared them with those nf umbelliferone (UMB), a structural analog of AUR that is virtually inactive toward O-2(-) generation inhibition. Double pre-treatments of mouse skin with AUR, but not UMB, markedly suppressed edema formation, hydrogen peroxide production, leukocyte infiltration, and the rate of proliferating cell nuclear antigen-stained cells. These inhibitory effects by AUR are attributable to its selective blockade of the activation stage, as revealed by single pre-treatment experiments. In a murine macrophage line, RAW 264.7, AUR significantly attenuated the lipopolysaccharide-induced protein expression of inducible isoforms of both nitric oxide synthase and cyclooxygenase, with decreased production of nitrite anion and prostaglandin E-2, and yet suppressed the release of tumor necrosis factor-alpha. Conversely, UMB did not show any inhibitory effect, This contrasting activity profile between AUR and UMB was rationalized to be a result of their distinct differences in cellular uptake efficiencies, i.e. the geranyloxyl group in AUR was found to play an essential role in incorporation Thus, our findings indicate that AUR is an effective agent to attenuate the biochemical responsiveness of inflammatory leukocytes, which may be essential for a greater understanding of the action mechanism that underlies its inhibition of inflammation-associated carcinogenesis.

    DOI: 10.1093/carcin/21.10.1843

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  • A simple phenolic antioxidant protocatechuic acid enhances tumor promotion and oxidative stress in female ICR mouse skin: dose- and timing-dependent enhancement and involvement of bioactivation by tyrosinase

    Y Nakamura, K Torikai, Y Ohto, A Murakami, T Tanaka, H Ohigashi

    CARCINOGENESIS   21 ( 10 )   1899 - 1907   2000年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The modifying effects of topical application of the phenolic antioxidant protocatechuic acid (PA) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse skin tumor promotion were investigated. Dimethylbenz[a]anthracene-initiated female ICR mice were treated with TPA (1.6 nmol) twice weekly for 20 weeks to promote papilloma formation. Pre-treatment with 16 nmol PA 30 min prior to each TPA treatment significantly inhibited the number of papillomas per mouse by 52% (P &lt; 0.05). On the other hand, PA pre-treatment at a high dose (1600 nmol) significantly enhanced tumor numbers by 38% (P &lt; 0.05). Interestingly, in the group treated with a quite high dose (20000 nmol) of PA 5 min prior to each TPA application, the average number of tumors per mouse was reduced by 38%, whereas the same PA dose 3 h before TPA treatment significantly enhanced tumor numbers by 84% (P &lt; 0.01). These results suggested that topically applied PA was converted to compound(s) lacking antioxidative properties and/or rather possessing the potential to enhance tumor development. A similar tendency was also observed in the short-term experiment of TPA-induced inflammation and oxidative stress; i.e. two groups pretreated with PA at 20 000 nmol, 30 min and 3 h before TPA treatment, did not show suppression or even significantly enhanced TPA-induced leukocyte infiltration, H2O2 generation, thiobarbituric acid-reacting substances level and proliferating cell nuclear antigen index, while PA treatment together with TPA significantly suppressed these parameters. Treatment with a high dose (20 000 nmol) of PA alone for 3 h enhanced oxidative stress by reducing glutathione levels in mouse skin, which was counteracted by the tyrosinase inhibitor arbutin, Oxidative stress responses such as leukocyte infiltration and H2O2 generation were also counteracted by arbutin, These results suggested that tyrosinase-dependent oxidative metabolism of PA was at least partially involved in the enhanced effects of PA on TPA-induced inflammatory responses and thus tumor promotion.

    DOI: 10.1093/carcin/21.10.1899

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  • A glutathione S-transferase inducer from papaya, rapid screening, identification and structure-activity relationship of isothiocyanates

    Y Nakamura, Y Morimitsu, T Uzu, H Ohigashi, A Murakami, Y Naito, Y Nakagawa, T Osawa, K Uchida

    CANCER LETTERS   157 ( 2 )   193 - 200   2000年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    We have developed a simple system for rapid detection and measurement of glutathione S-transferase placental form (GSTP1) that detoxify polycyclic aromatic hydrocarbons using the cultured rat normal liver epithelial cell line, (RL34) cells. Survey of fruit extracts for GST inducing ability identified both papaya and avocado as significant sources. Benzyl isothiocyanate (BITC) was isolated from papaya methanol extract as a principal inducer of GST activity. Further, the GST inducing ability of a total of 20 isothiocyanates (ITCs) and their derivatives was investigated. Some ITCs showed significant induction, and BITC was one of the most potent inducers among all compounds tested in the present study. The modification of isothiocyanate group (-NCS) or introduction of substituent group to the a-carbon modifies GST induction. Moreover, a significant correlation (P &lt; 0.01, r = 0.913) between the GST activity enrichment and GSTP1 protein induction by ITCs was observed. We also indicated that phenethyl ITC and nitrophenyl ITC, potently inducing GST activity, but not inactive benzyl isocyanate, are potential inducers of intracellular reactive oxygen intermediates (ROIs). Our system of GSTP1 induction is appropriate for the chemical research such as screening and identification of novel type of inducers as well as the structure-activity relationship studies, providing mechanistic insight into essential structural elements for GSTP1 induction. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

    DOI: 10.1016/S0304-3835(00)00487-0

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  • Inhibitory effect of citrus nobiletin on phorbol ester-induced skin inflammation, oxidative stress, and tumor promotion in mice

    A Murakami, Y Nakamura, K Torikai, T Tanaka, T Koshiba, K Koshimizu, S Kuwahara, Y Takahashi, K Ogawa, M Yano, H Tokuda, H Nishino, Y Mimaki, Y Sashida, S Kitanaka, H Ohigashi

    CANCER RESEARCH   60 ( 18 )   5059 - 5066   2000年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC CANCER RESEARCH  

    The intake of citrus fruits has been suggested as a way to prevent the development of some types of human cancer. Nitric oxide (NO) is closely associated with the processes of epithelial carcinogenesis. We attempted a search for NO generation inhibitors in Cia rcs unshiu. The active constituent was traced by an activity-guiding separation. NO and superoxide (O(2)(-)) generation was induced by a combination of lipopolysaccharide and IFN-gamma in mouse macrophage RAW 264.7 cells, and by 12-O-tetradecanoylphorbol-13-acetate (TPA) in differentiated human promyelocyte HL-60, respectively. Expression of inducible NO synthase and cyclooxygenase 2 proteins were detected by Western blotting, The in vivo anti-inflammatory and antitumor promoting activities were evaluated by topical TPA application to ICR mouse skin with measurement of edema formation, epidermal thickness, leukocyte infiltration, hydrogen peroxide production, and the rate of proliferating cell nuclear antigen-stained cells. As a result, nobiletin, a polymethoxyflavonoid, was identified as an inhibitor of both NO and O(2)(-) generation, Nobiletin significantly inhibited two distinct stages of skin inflammation induced by double TPA application [first stage priming (leukocyte infiltration) and second stage activation (oxidative insult by leukocytes)] by decreasing the inflammatory parameters. It also suppressed the expression of cyclooxygenase-2 and inducible NO synthase proteins and prostaglandin E(2) release. Nobiletin inhibited dimethylbenz[a]anthracene (0.19 mu mol)/TPA (1.6 nmol)-induced skin tumor formation at doses of 160 and 320 nmol by reducing the number of tumors per mouse by 61.2% (P &lt; 0.001) and 75.7% (P &lt; 0.001), respectively. The present study suggests that nobiletin is a functionally novel and possible chemopreventive agent in inflammation-associated tumorigenesis.

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  • 4-hydroxy-2-nonenal, the end product of lipid peroxidation, is a specific inducer of cyclooxygenase-2 gene expression

    T Kumagai, Y Kawamoto, Y Nakamura, Hatayama, I, K Satoh, T Osawa, K Uchida

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   273 ( 2 )   437 - 441   2000年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC  

    Cyclooxygenase-2 (COX-2), an enzyme responsible for catalyzing the committed step in prostanoid biosynthesis, is the product of an immediate early gene capable of being upregulated by diverse stimuli. Based on the experimental evidence that oxidative stress is associated with the upregulation of COX-2, we evaluated the effect of the oxidized fatty acid metabolites on COX-2 induction in rat liver epithelial RL34 cells, Among the compounds tested, only 4-hydroxy-2-nonenal (HNE), a highly mutagenic and genotoxic aldehyde generated during oxidative stress, dramatically induced COX-2. Enhanced gene expression of COX-2 by treatment with HNE was evident as a drastic elevation of the mRNA level. We also found that intracellular glutathione status was strictly related to HNE-induced COX-2 expression. These findings suggest the presence of a signaling pathway in the cellular response mediated by locally produced lipid peroxidation products under oxidative stress. (C) 2000 Academic Press.

    DOI: 10.1006/bbrc.2000.2967

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  • Novel nitric oxide and superoxide generation inhibitors, persenone A and B, from avocado fruit

    OK Kim, A Murakami, Y Nakamura, N Takeda, H Yoshizumi, H Ohigashi

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   48 ( 5 )   1557 - 1563   2000年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    One known, (2R)-(12Z,15Z)-2-hydroxy-4-oxoheneicosa-12,15-dien-1-yl acetate (1), and two novel compounds, persenone A (2) and B (3), have been isolated from avocado fruit (Persea americana P. Mill), as inhibitors of superoxide (O-2(-)) and nitric oxide (NO) generation in cell culture systems. They showed marked inhibitory activities toward NO generation induced by lipopolysaccharide in combination with interferon-gamma in mouse macrophage RAW 264.7 cells. Their inhibitory potencies of NO generation (1, IC50 = 3.6; 2, IC50 = 1.2; and 3, IC50 = 3.5 mu M) were comparable to or higher than that of a natural NO generation inhibitor, docosahexaenoic acid (DKA; IC50 = 4.3 mu M). Furthermore, compounds 1-3 and DHA markedly suppressed tumor promoter 12-O-tetradecanoylphorbol-13-acetate-induced O-2(-) generation in differentiated human promyelocytic HL-60 cells (1, EC50 = 33.7; 2, IC50 = 1.4; 3, IC50 = 1.8; and DHA, IC50 = 10.3 mu M). It is notable that they were found to be suppressors of both NO- and O-2(-)-generating biochemical pathways but not to be radical scavengers. The results indicate that these compounds are unique antioxidants, preferentially suppressing radical generation, and thus may be promising as effective chemopreventive agent candidates in inflammation-associated carcinogenesis.

    DOI: 10.1021/jf990977r

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  • Cyclopentenone prostaglandins as potential inducers of phase II detoxification enzymes. 15-deoxy-δ prostaglandin J2-induced expression of glutathione S-transferases

    Yoshiyuki Kawamoto, Yoshimasa Nakamura, Yuko Naito, Yasuyoshi Torii, Takeshi Kumagai, Toshihiko Osawa, Hajime Ohigashi, Kimihiko Satoh, Masayoshi Imagawa, Koji Uchida

    Journal of Biological Chemistry   275 ( 15 )   11291 - 11299   2000年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Exposure of cells to a wide variety of chemoprotective compounds confers resistance to a broad set of carcinogens. For a subset of the chemoprotective compounds, protection is generated by an increase in the abundance of protective enzymes, such as glutathione S-transferases (GSTs). In the present study, we developed a cell culture system that potently responds to phenolic antioxidants and found that antitumor prostaglandins (PGs) are potential inducers of GSTs. We screened primary hepatocytes and multiple cell lines for inducing GST activity upon incubation with the phenolic antioxidant (tert- butylhydroquinone) and found that rat liver epithelial RL34 cells most potently responded. Based on an extensive screening of diverse chemical agents on the induction of GST activity in RL34 cells, the J2 series of PGs, 15-deoxy-Δ 12,14 -prostaglandin J2 (15-deoxy-Δ 12,14 PGJ2) in particular, were found to be potential inducers of GST. Enhanced gene expression of Class π GST isozyme (GSTP1) by 15-deoxy-Δ 12,14 -PGJ2 was evident as a drastic elevation of the mRNA level. Hence, we examined the molecular mechanism underlying the 15-deoxyΔ 12,14 -PGJ2-induced GSTP1 gene expression. From functional analysis of various deletion mutant genes, we found that the 15-deoxy-Δ 12,14 -pGJ2 reponse element was localized in a region containing a GSTP1 enhancer I (GPEI) that consists of two imperfect phorbol 12-0-tetradecanoylphorbol-13-acetate response elements. When the GPEI was combined with the minimum GSTP1 promoter, the element indeed showed an enhancer activity in response to 15-deoxy-Δ 12,14 -PGJ2. Point mutations of either of the two imperfect 12-O-tetradecanoylphorbol-13-acetate response elements in GPEI completely abolished the enhancer activity. Gel mobility shift assays demonstrated that 15-deoxy-Δ 12,14 -pGJ2 specifically stimulated the binding of nuclear proteins including the transcription factor c-Jun, but not Nrf2, to GPEI. These results suggest that 15-deoxy- Δ 12,14 -PGJ2 induces the expression of the rat GSTP1 gene through binding of proteins, including c-Jun, to a specific GPEI.

    DOI: 10.1074/jbc.275.15.11291

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  • Search for Naturally-occurring Antioxidative Chemopreventors on the Basis of the Involvement of Leukocyte-derived Reactive Oxygen Species in Carcinogenesis

    Yoshimasa Nakamura, Akira Murakami, Hajime Ohigashi

    Asian Pacific Journal of Cancer Prevention   1   115 - 120   2000年1月

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    Chemoprevention with food phytochemicals is currently regarded as one of the most attractive strategies for cancer control. We have been continuously working on the identification and characterization of chemopreventive phytochemicals extracted from a diverse range of edible plants. Recently, we have utilized a convenient assay, the tumor promoter-induced superoxide generation test, with differentiated HL-60 cells for primary screening, and performed chemical studies of antioxidative food phytochemicals. Here, we report our criteria for evaluation of new types of chemopreventors on the basis of the involvement of leukocyte-derived reactive oxygen in carcinogenesis.

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  • Redox regulation of glutathione S-transferase induction by benzyl isothiocyanate: Correlation of enzyme induction with the formation of reactive oxygen intermediates

    Y Nakamura, H Ohigashi, S Masuda, A Murakami, Y Morimitsu, Y Kawamoto, T Osawa, M Imagawa, K Uchida

    CANCER RESEARCH   60 ( 2 )   219 - 225   2000年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC CANCER RESEARCH  

    Here we report the molecular mechanism underlying the induction of glutathione S-transferase (GST) in rat liver epithelial RL34 cells treated with a cancer chemopreventive isothiocyanate compound, benzylisothiocyanate (BITC), BITC was found to significantly induce GST activity in RL34 cells. Northern and Western blot analyses demonstrated that BITC specifically enhanced the production of the class pi GST isozyme (GSTP1), Our studies demonstrated for the first time that the addition of BITC to the cells resulted in an immediate increase in the reactive oxygen intermediates (ROIs) detected by a fluorescence probe, 2',7'-dichlorofluorescin diacetate. The level of the ROIs in the cells treated with BITC (10 mu M) was similar to 50-fold higher than those in the control cells. Furthermore, glutathione depletion by diethyl maleate significantly enhanced BITC-induced ROI production and accelerated the BITC-induced elevation of the GST activity, whereas pretreatment of the cells with glutathione inhibited both the ROI production and GST induction. The structure-activity relationship of the isothiocyanates also indicated that the ROI-producing activities closely correlated with their GST-inducing potencies. Moreover, the GSTP1 enhancer I-containing region was found to be essential for induction of the GSTP1 gene by intracellular ROI inducers such as BITC and diethyl maleate. These data suggest the involvement of the redox regulation on the induction of GSTP1 by BITC.

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  • Suppressive effects of citrus fruits on free radical generation and nobiletin, an anti-inflammatory polymethoxyflavonoid

    A Murakami, Y Nakamura, Y Ohto, M Yano, T Koshiba, K Koshimizu, H Tokuda, H Nishino, H Ohigashi

    BIOFACTORS   12 ( 1-4 )   187 - 192   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Citrus fruit intake is known to be associated with a reduction of cancer incidence. Free radicals, including superoxide (O(2)(-)) and nitric oxide (NO), are involved in some epithelial carcinogenesis processes. In the present study, we screened thirty-one citrus fruits for their suppressive activities toward three lines of free radical generating systems: 1) O(2)(-) generation by the xanthine (XA)-xanthine oxidase (XOD) system; 2) O(2)(-) generation induced by 1 2-O-tetradecanoylphorbol-13-acetate (TPA) in differentiated human promyelocytic HL-60 cells; and 3) NO generation in murine macrophage RAW264.7 cells stimulated with lipopolysaccharide (LPS) and interferon (IFN)-gamma. As a result, the inhibitory activities of peel parts were largely found to be higher than those of the corresponding juice sac parts. In particular, the peel portion of Dancy tangerine (Citrus tangerina) showed marked anti-oxidative activities in these systems. In addition, nobiletin, a polymethoxyflavonoid isolated from C. nobilis, showed a higher anti-inflammatory activity than indomethacin in a TPA-induced edema formation test in mouse ears. These results indicate that citrus fruits could be notable sources of anti-oxidative, anti-inflammatory, and cancer preventive compounds.

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  • Kurosu, a traditional vinegar produced from unpolished rice, suppresses lipid peroxidation in vitro and in mouse skin

    Shoko Nishidai, Yoshimasa Nakamura, Koji Torikai, Mikako Yamamoto, Nobuhiro Ishihara, Hirotaka Mori, Hajime Ohigashi

    Bioscience, Biotechnology and Biochemistry   64 ( 9 )   1909 - 1914   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The in vitro antioxidative activities of various kinds of vinegar were investigated by using a linoleic acid autoxidation model detected by the thiobarbituric acid (TBA) method and the 1,1-diphenyl-2-picrylhydrazyl radical system. An ethyl acetate extract of Kurosu (EK), a vinegar made from unpolished rice, exhibited the highest antioxidative activity in both systems. EK (5 mg) inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced edema formation (14%) and myeloperoxidase activity (52%, P&lt
    0.01) in female ICR mouse skin. Furthermore, EK significantly suppressed double TPA application-induced H2O2 generation (53%, P&lt
    0.01) and lipid peroxidation determined by the TBA-reacting substance level (95%, P&lt
    0.01). In a two-stage carcinogenesis experiment with dimethylbenz[a]anthracene/TPA, EK significantly reduced the number of tumors per mouse by 36% (P&lt
    0.05) at 15 weeks after promotion. These results suggest that the antitumor-promoting effect may be partially due to the antioxidative properties of EK such as the decomposition of free radicals and interference with free radical-generating leukocytes. © 2000, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.64.1909

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  • An avocado constituent, persenone A, suppresses expression of inducible forms of nitric oxide synthase and cyclooxygenase in macrophages, and hydrogen peroxide generation in mouse skin

    Oe Kyung Kim, Akira Murakami, Daisuke Takahashi, Yoshimasa Nakamura, Koji Torikai, Ha Won Kim, Hajime Ohigashi

    Bioscience, Biotechnology and Biochemistry   64 ( 11 )   2504 - 2507   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We investigated the suppressive effects of an avocado constituent, persenone A, on lipopolysaccharide- and interferon-γ-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) in a mouse macrophage cell line RAW 264.7. Persenone A at concentration of 20 μM almost completely suppressed both iNOS and COX-2 protein expression. In mouse skin, double treatments with persenone A (810 nmol) significantly suppressed double 12-O-tetradecanoylphorbol-13-acetate (TPA, 8.1 nmol) application-induced hydrogen peroxide (H2O2) generation. Treatment with persenone A before the second TPA treatment was sufficient to inhibit H2O2 generation, while the first treatment was not. This study thus suggests that persenone A is a possible agent to prevent inflammation-associated diseases including cancer. © 2000, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.64.2504

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  • Inhibition by (-)-Persenone A-related compounds of nitric oxide and superoxide generation from inflammatory leukocytes

    Oe Kyung Kim, Akira Murakami, Yoshimasa Nakamura, Ha Won Kim, Hajime Ohigashi

    Bioscience, Biotechnology and Biochemistry   64 ( 11 )   2500 - 2503   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We have previously reported that persenone A, isolated from avocado fruit, is an effective inhibitor of both nitric oxide (NO) and superoxide (O2−) generation in cell culture systems. In this study, we have prepared four persenone A-related compounds and examined their inhibition of NO and O2− generation from inflammatory leukocytes. Some structural importance in persenone A to attenuate free radical generation is discussed. © 2000, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.64.2500

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  • Synthesis and tumor-promoting activities of 12-Epi-phorbol-12,13-dibutyrate

    Kazuhiro Irie, Akifumi Nakahara, Yasuyuki Ikawa, Minoru Tanaka, Yu Nakagavva, Yoshimasa Nakamura, Hajime Ohigashi, Paul A. Wender

    Bioscience, Biotechnology and Biochemistry   64 ( 11 )   2429 - 2436   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    12-Epi-phorbol-12,13-dibutyrate (1), the C12-epimer of the most frequently used phorbol ester probe, phorbol-12,13-dibutyrate (PDBu), has been synthesized from phorbol in 9 steps in order to investigate the structural requirements for tumor-promoting activity. Compound 1 showed about 100-fold weaker in vitro biological activities related to in vivo tumor promotion, Epstein-Barr virus early antigen (EBV-EA)-inducing ability, superoxide (O2 −) generation-inducing ability, and binding to the protein kinase C (PKC) regulatory domain surrogate peptides. The results indicated that the β-stereochemistry at position 12 of the phorbol skeleton is important for optimal activity. Binding selectivity to each PKC C1 domain of 1 was almost equal to that of PDBu. © 2000, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.64.2429

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  • Nitric oxide synthase is induced in tumor promoter-sensitive, but not tumor promoter-resistant, JB6 mouse epidermal cells cocultured with interferon-γ-stimulated RAW 264.7 Cells: The role of tumor necrosis factor-α

    A. Murakami, K. Kawabata, T. Koshiba, G. Gao, Y. Nakamura, K. Koshimizu, H. Ohigashi

    Cancer Research   60 ( 22 )   6326 - 6331   2000年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Expression of inducible nitric oxide synthase (iNOS) has been reported to be involved in certain organs of potential tumorigenesis, including the stomach and colon. The mechanisms for iNOS expression in epithelial cells, however, are not fully understood. In the present study, we investigated the role of macrophages in epithelial iNOS expression by coculturing a stimulated murine macrophage-like cell line, RAW 264.7, with either tumor promoter-sensitive (P+) or promoter-resistant (P-) JB6 murine epidermal cells. After monoculture, treatment of RAW 264.7 cells with IFN-γ for 24 h generated a large amount of nitrite (NO 2 - ), as reported previously, whereas no increase in NO 2 - concentration was observed in the IFN-γ-treated P+ or P- subclones. Interestingly, when IFN-γ-treated RAW 264.7 cells were cocultured with P+ but not P- cells, we observed a marked increase in NO 2 - concentration (30.8 ± 3.6 μM), which significantly exceeded (P &lt; 0.01) the sum of the concentrations (20.0 ± 2.3 μM) added from each cell line monoculture. Western blotting analysis revealed that, after coculture, iNOS protein was up-regulated 55-fold more than the control in JB6 P+ but not in P- cells. IFN-γ-treated RAW 264.7 cells secreted proinflammatory cytokines, including tumor necrosis factor (TNF)-α and interleukin (IL)-1β. The addition of IFN-γ-treated RAW 264.7 cell-conditioned media to P+ subclones led to a significant enhancement of NO 2 - formation that was diminished by the TNF-α-specific but not IL-1β-specific antibody. When combined with IFN-γ, the recombinant TNF-α (1-100 ng/ml) enhanced NO 2 - formation in JB6 P+ cells, whereas IL-1β (1-100 ng/ml) did not. These results led us to conclude that IFN-γ-treated RAW 264.7 cells release TNF-α to induce iNOS expression in promoter-sensitive JB6 cells. Thus, we propose the hypothesis that macrophages stimulate neoplastic cells with TNF-α via a paracrine loop to induce epithelial iNOS protein expression.

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  • A diacetylenic spiroketal enol ether epoxide, AL-1, from Artemisia lactiflora inhibits 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion possibly by suppression of oxidative stress

    Y Nakamura, N Kawamoto, Y Ohto, K Torikai, A Murakami, H Ohigashi

    CANCER LETTERS   140 ( 1-2 )   37 - 45   1999年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    The inhibitory effects of the diacetylenic spiroketal enol ether epoxide AL-1 from Artemisia lactiflora on a variety of tumor promoter-induced biological responses such as oxidative stress as well as tumor promotion in ICR mouse skin were investigated. AL-1 inhibited TPA-induced intracellular peroxide formation in differentiated HL-60 cells, suggesting that this suppression might be attributable to the inhibition of O-2(-) generation. In a double TPA application system in mouse skin, double pretreatments of AL-1 (810 nmol) significantly suppressed double TPA application-induced H2O2 generation. Pretreatment of AL-1 only before the second TPA treatment was sufficient to inhibit, while only with first treatment was not. From these results we concluded that AL-1 is a specific inhibitor of the activation phase in H2O2 production induced by double TPA treatments. In addition, AL-1 strongly inhibited tumor promoter-induced Epstein-Barr virus (EBV) activation in Raji cells (IC50 = 0.5 mu M), which was comparable to or even stronger than that of curcumin, a well-known antioxidative chemopreventer from turmeric. In a two-stage carcinogenesis experiment with TPA (topical application at 1.6 nmol) and 7,12-dimethylbenz[a]anthracene (DMBA, at 0.19 mu mol) in ICR mouse skin, topical application of AL-1 (at 160 nmol) significantly reduced tumor incidence, the numbers of tumors per mouse, and edema formation by 58% (P &lt; 0.01 in t-test), 20% (P &lt; 0.005 in chi(2)-test) and 42% (P &lt; 0.01), respectively. These results together indicate that an inhibitor of O-2(-) generation is an effective chemopreventer of mouse skin carcinogenesis by their antioxidative property. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

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  • Activation of stress signaling pathways by the end product of lipid peroxidation - 4-hydroxy-2-nonenal is a potential inducer of intracellular peroxide production

    K Uchida, M Shiraishi, Y Naito, Y Torii, Y Nakamura, T Osawa

    JOURNAL OF BIOLOGICAL CHEMISTRY   274 ( 4 )   2234 - 2242   1999年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    In the present study, we studied the signal transduction mechanism that is involved in the expression of c-Jun protein evident after exposure of rat liver epithelial RL34 cells to the major end product of oxidized fatty acid metabolism, 4-hydroxy-2-nonenal (HNE). HNE treatment of the cells resulted in depletion of intracellular glutathione (GSH) and in the formation of protein-bound HNE in plasma membrane. In addition, HNE strongly induced intracellular peroxide production, suggesting that HNE exerted oxidative stress on the cells. Potent expression of c-Jun occurred within 30 min of HNE treatment, which was accompanied by a time-dependent increase in activator protein-1 (AP-1) DNA binding activity. We found that HNE caused an immediate increase in tyrosine phosphorylation in RL34 cells, In addition, HNE strongly induced phosphorylation of c-Jun N-terminal kinases (JNK) and p38 mitogen-activated protein kinases and also moderately induced phosphorylation of extracellular signal-regulated kinases. The phosphorylation of JNK was accompanied by a rapid and transient increase in JNK and p38 activities, whereas changes in the activity of extracellular signal-regulated kinase were scarcely observed. GSH depletion by L-buthionine-S,R-sulfoximine, a specific inhibitor of GSH biosynthesis, only slightly enhanced peroxide production and JNK activation, suggesting that HNE exerted these effects independent of GSH depletion. This and the findings that (i) HNE strongly induced intracellular peroxide production, (ii) HNE-induced JNK activation was inhibited by pretreatment of the cells with a thiol antioxidant, N-acetylcysteine, and (iii) H2O2 significantly activated JNK support the hypothesis that pro-oxidants play a crucial role in the HNE-induced activation of stress signaling pathways. In addition, we found that, among the inhibitors of tyrosine kinases, cyclooxygenase, and Ca2+ influx, only quercetin exerted a significant inhibitory effect on HNE-induced JNK activation. In light of the JNK-dependent induction of c-jun transcription and the AP-1-induced transcription of xenobiotic-metabolizing enzymes, these data may show a potential critical role for JNK in the induction of a cellular defense program against toxic products generated from lipid peroxidation.

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  • Isolation and identification of acetylenic spiroketal enol ethers from Artemisia lactiflora as inhibitors of superoxide generation induced by a tumor promoter in differentiated HL-60 cells

    Y Nakamura, Y Ohto, A Murakami, S Jiwajinda, H Ohigashi

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   46 ( 12 )   5031 - 5036   1998年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    Six acetylenic compounds were isolated from the leaves of Artemisia lactiflora (Compositae), an edible plant of Thailand. Four of them were identified as the stereoisomers of 3,4-epoxy-2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4.5]decane and its derivatives, whose planar structures have already been reported. The other two were identified as novel chlorohydrin derivatives of the corresponding diacetylene spiroketal enol ethers. The inhibitory effects of the polyacetylenes isolated in the present study together with genistein on TPA-induced O-2(-) generation were examined. It was revealed that an acyloxyl group at the C-2 position enhanced-the inhibitory effect, while the absolute configurations at C-5, -6, and -7 were not important. While polyacetylenes are known to possess several biological roles in various ecosystems, we first found inhibitory effects of the diacetylenes on TPA-induced O-2(-) generation in differentiated HL-60 cells.

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  • Suppression of tumor promoter-induced oxidative stress and inflammatory responses in mouse skin by a superoxide generation inhibitor 1 '-acetoxychavicol acetate

    Y Nakamura, A Murakami, Y Ohto, K Torikai, T Tanaka, H Ohigashi

    CANCER RESEARCH   58 ( 21 )   4832 - 4839   1998年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC CANCER RESEARCH  

    Double applications of phorbol eaters trigger excessive reactive oxygen species (ROS) production in mouse skin. previously reported data suggest that the two applications induce distinguishable biochemical events, namely, priming and activation. The former is characterized as a recruitment of inflammatory cells, such as neutrophils, by chemotactic factors to inflammatory regions and edema formation. The latter is responsible for ROS generation. Thus, inhibitory effects of 1'-acetoxychavicol acetate (ACA), previously reported to be a superoxide generation inhibitor is vitro, on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced oxidative stress and inflammatory responses in mouse skin model were examined using a double application of ACA. We demonstrated that two pretreatments and pretreatment with ACA (810 mmol) in the activation phase suppressed double TPA application-induced H2O2 formation in mouse skin, ACA exhibited no inhibitory effects on edema formation and the enhancement of myeloperoxidase activity during the first TPA treatment, whereas the anti-inflammatory agent genistein administered at the same dose inhibited both biomarkers, No inhibitory potential of ACA for TPA-induced H2O2 formation in the priming phase was confirmed. On the other hand, in the in vitro study, ACA inhibited ROS generation in differentiated HL-60 cells more strongly than did 1'-hydroxychavicol, which showed no inhibition by pretreatment in the activation phase In addition, allopurinol did not inhibit double TPA application-induced H2O2 formation in mouse skin, These findings suggest that the NADPH oxidase system of neutrophils rather than the epithelial xanthine oxidase system is dominant for the O-2(-)-generating potential in double TPA-treated mouse skin. ACA significantly inhibited mouse epidermis thiobarbituric acid-reacting substance formation, known as an overall oxidative damage biomarker, Moreover, histological studies demonstrated that ACA inhibited double TPA treatment-induced morphological changes reflecting inflammatory response, such as edema formation, leukocyte infiltration, hyperplasia, and cell proliferation. Furthermore, pretreatment with ACA but not 1'-hydroxychavicol in the activation phase inhibits double TPA application-induced increases in both number of leukocytes and proliferating cell nuclear antigen index. These results suggested that ROS from leukocytes including O-2(-) plays an important role for continuous and excessive production of chemotactic factors, leading to chronic inflammation and hyperplasia, which are inhibitable by ACA. Thus, we concluded that O-2(-) generation inhibitors are agents that effectively inhibit oxidative stress and inflammatory responses in mouse skin.

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  • Superoxide scavenging activity of rosmarinic acid from Perilla frutescens Britton var. acuta f. viridis

    Y Nakamura, Y Ohto, A Murakami, H Ohigashi

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   46 ( 11 )   4545 - 4550   1998年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    Rosmarinic acid (RoA) has been isolated in high yield (0.1%) from the leaves of Perilla frutescens Britton var, acuta f. viridis, one of the most common garnishes in Japan, as a superoxide scavenger in a xanthine/xanthine oxidase system. The scavenging activity of RoA was significantly higher than that of ascorbic acid (AA). The structure-activity relationships using RoA, caffeic acid (CaA), and its related compounds indicated that the presence of an ortho dihydroxyphenyl group is essential for the scavenging effect. In addition, the conjugated double bond in the C3 carbon chain is important for enhancing the effect. The activity of RoA was then shown to be due to the additive effect of both CaA and (3,4-dihydroxyphenyl)lactic acid units, both of which are hydrolyzed products of RoA. RoA significantly inhibited both intracellular superoxide and peroxide formation in differentiated HL-60 cells, suggesting that RoA effectively exhibited antioxidative activity in the biological systems through the scavenging of superoxide.

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  • Screening of edible Japanese plants for nitric oxide generation inhibitory activities in RAW 264.7 cells

    OK Kim, A Murakami, Y Nakamura, H Ohigashi

    CANCER LETTERS   125 ( 1-2 )   199 - 207   1998年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    A total of 48 (60 test samples) species of plants commonly eaten in Japan were randomly collected and their methanol extracts were tested for in vitro nitric oxide (NO) generation inhibitory activities in a murine macrophage cell line, RAW 264.7, stimulated with both lipopolysaccharide (LPS, 100 ng/ml) and interferon-gamma (IFN-gamma, 100 U/ml). Seventeen (28.3%) of the 48 extracts strongly inhibited NO generation at a concentration of 200 mu g/ml by 70% or more with significant cell viability (&gt;50%). The extracts from avocado, tare, red turnip, sereves, komatsuna, basil, mitsuba and Chinese mustard markedly inhibited iNOS activity. These results suggest that a wide variety of edible plants in Japan contain the secondary metabolites carrying cancer preventive activity through reduction of excess amounts of NO. (C) 1998 Elsevier Science Ireland Ltd.

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  • Synthesis and Biological Activities of (-)-6-n-Octyl-indolactam-V, a New Potent Analogue of the Tumor Promoter (-)-Indolactam-V

    Yu Nakagawa, Kazuhiro Irie, Yoshimasa Nakamura, Hajime Ohigashi, Hideo Hayashi

    Bioscience, Biotechnology and Biochemistry   62 ( 8 )   1568 - 1573   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    (-)-Indolactam-V (1) without a hydrophobic chain at positions 6 and 7 of the indole ring is a weak tumor promoter compared with teleocidin Bs. To investigate the effects of the hydrophobic substituent at position 6 of teleocidin Bs, we synthesized (-)-6-n-octyl-indolactam-V (2) by a palladium-catalyzed coupling reaction from (-)-6-bromo-indolactam-V (7) which had been obtained by microbial conversion with Streptoverticillium blastmyceticum NA34-17 as the key step. (-)-7-n-Octyl-indolactam-V (3) with potent biological activities comparable to those of teleocidin Bs was similarly synthesized from (-)-7-bromo-indolactam-V as a positive control. Compound 2 showed similar biological activities to those of 3, indicating that the effect of the hydrophobic substituent at position 6 of 1 was identical to that at position 7. © 1998, Taylor &amp
    Francis Group, LLC. All rights reserved.

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  • Inhibitory effects of curcumin and tetrahydrocurcuminoids on the tumor promoter-induced reactive oxygen species generation in leukocytes in vitro and in vivo

    Yoshimasa Nakamura, Yoshimi Ohto, Akira Murakami, Toshihiko Osawa, Hajime Ohigashi

    Japanese Journal of Cancer Research   89 ( 4 )   361 - 370   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japanese Cancer Association  

    The inhibitory effects of curcumin and two tetrahydrocurcuminoids on tumor promoter-induced oxidative stress in vitro and in vivo were investigated. Curcumin, tetrahydrocurcumin (THC) and dihydroxytetrahydrocurcumin (DHTHC) exhibited significant inhibitory effects on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced O2 - generation in differentiated HL-60 cells. The inhibitory activity of THC was weaker than that of curcumin. This tendency was the inverse of the results of previous studies on in vitro antioxidative activity against lipid peroxidation. The curcuminoids inhibited TPA-induced intracellular peroxide formation in differentiated HL-60 cells. THC exhibited much weaker inhibition of intracellular peroxide formation than curcumin, suggesting that this inhibition might be attributable to the inhibition of O2 - generation. The inhibitory effects of curcuminoids on TPA-induced H2O2 formation in female ICR mouse skin were further examined using the double-TPA-application model. Each TPA application induces two distinct biochemical events, 1) recruitment of inflammatory cells to the inflammatory regions and 2) activation of oxidant-producing cell. Double pretreatment of mice with curcuminoids before each TPA treatment significantly suppressed double TPA application-induced H2O2 formation in the mouse skin. Coadministrations of curcumin with either first or second TPA treatment significantly inhibited H2O2 formation. In addition, THC tends to show weaker inhibitory activities than curcumin in bioassays related to tumor promotion, i.e., inhibition of tumor promoter-induced inflammation in mouse skin and Epstein-Barr virus activation. These tendencies were parallel to those in the tumor-suppressive potential of curcumin and THC in mouse skin, as previously reported. Thus, we concluded that curcuminoids significantly suppress TPA-induced oxidative stress via both interference with infiltration of leukocytes into the inflammatory regions and inhibition of their activation.

    DOI: 10.1111/j.1349-7006.1998.tb00572.x

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  • Cellular response to the redox active lipid peroxidation products: Induction of glutathione S-transferase P by 4-hydroxy-2-nonenal

    A Fukuda, Y Nakamura, H Ohigashi, T Osawa, K Uchida

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   236 ( 2 )   505 - 509   1997年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    Membrane lipid peroxidation is known to produce various aldehydic compounds which cause a wide range of biological effects including heart disease, aging, and cancer. To investigate the effect of lipid peroxidation products on the expression of glutathione S-transferases (GSTs), which catalyze the conjugation of reactive chemicals with glutathione and play an important role in protecting cells, normal rat liver epitherial cells (RL34) were exposed to a variety of aldehydic compounds. We found that the GST activity in RL34 cells was induced by alpha,beta-unsaturated aldehydes, such as acrolein (1.3-fold), crotonaldehyde (1.3-fold), 4-hydroxy-2-hexenal (HHE) (1.4-fold), and 4-hydroxy-2-nonenal (HNE) (1.7-fold). The induction of GST activity by HNE was time-dependent, reaching a plateau after 16 h. The immunoblot analysis using the polyclonal antibodies against GST isozymes demonstrated that GST-P (pi-class), a well-known tumor marker, was significantly induced 16 h after the HNE treatment. Also, immunostaining for the presence of GST-P confirmed the enhanced expression of GST-P in the cytoplasm of the cells, Northern blot analysis revealed that the HNE treatment of RL34 cells for 1 h enhanced the expression of GST-P mRNA, which returned to the control level after 16 h. These data suggest that the induction of GST-P by HNE may represent an important cellular defense mechanism against oxidative injury. (C) 1997 Academic Press.

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  • Auraptene, a citrus coumarin, inhibits 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion in ICR mouse skin, possibly through suppression of superoxide generation in leukocytes

    A Murakami, W Kuki, Y Takahashi, H Yonei, Y Nakamura, Y Ohto, H Ohigashi, K Koshimizu

    JAPANESE JOURNAL OF CANCER RESEARCH   88 ( 5 )   443 - 452   1997年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE CANCER ASSOC  

    Coumarin-related compounds, auraptene and umbelliferone, have been isolated from the cold-pressed oil of natsumkian (Citrus natsudaidai HAYATA), and tested as inhibitors of tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced Epstein-Barr virus activation in Raji cells. The 50% inhibitory concentration (IC50) of auraptene (18 mu M) was almost equal to that of genistein. Umbelliferone, which lacks a geranyloxyl group present in auraptene, was less active (IC50 = 450 mu M). In a two-stage carcinogenesis experiment with 7,12-dimethylbenz[a]anthracene (topical application at 0.19 mu mol) and TPA (topical application at 1.6 nmol) in ICR mouse skin, topical application of auraptene (at 160 nmol) significantly reduced tumor incidence and the numbers of tumors per mouse by 27% (P &lt; 0.01) and 23% (P &lt; 0.05), respectively. Auraptene at a concentration of 50 mu M markedly suppressed superoxide (O-2(-)) generation induced by 100 nM TPA in differentiated human promyelocytic HL-60 cells. Having no O-2(-) scavenging potential, auraptene may inhibit the multicomponent NADPH oxidase system, Inhibition of intracellular hydroperoxide formation in differentiated HL-60 cells by auraptene was also confirmed by flow-cytometric analysis using 2',7'-dichlorofluorescein diacetate as a fluorescence probe. Quantitative analyses using high-performance liquid chromatography showed the occurrence of auraptene not only in both the peels and sarcocarps of natsumikan, but also in those of hassaku orange (C. hassaku) and grapefruit (C. paradisi), and even in their bottled fresh juice form. These results indicate that auraptene is a chemopreventer of skin tumorigenesis, and implies that suppression of leukocyte activation might be the mechanism through which it inhibits tumor promotion.

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  • Synthesis and Biological Activities of Indolactone-V, the Lactone Analogue of the Tumor Promoter (–)-Indolactam-V

    Yu Nakagawa, Kazuhiro Irie, Yoshimasa Nakamura, Hajime Ohigashi, Hideo Hayashi

    Bioscience, Biotechnology and Biochemistry   61 ( 8 )   1415 - 1417   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The tumor promoter (–)-indolactam-V (1) exists in two stable conformers (twist and sofa) due to isomerization of the amide group. Indolactone-V (2), the lactone analogue of 1, has been synthesized to investigate the effects of the amide group on its conformation and biological activities. Indolactone-V (2) existed only as the inactive sofa-like conformer, indicating that the amide group of 1 plays a critical role in formation of the active twist conformation. © 1997, Taylor &amp; Francis Group, LLC. All rights reserved.

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  • Inhibitory effect of pheophorbide α, a chlorophyll-related compound, on skin tumor promotion in ICR mouse

    Yoshimasa Nakamura, Akira Murakami, Koichi Koshimizu, Hajime Ohigashi

    Cancer Letters   108 ( 2 )   247 - 255   1996年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Anti-tumor-promoting activity of pheophorbide α (PPBα), a chlorophyll-related compound, was examined in a two-stage carcinogenesis experiment in ICR mouse skin by 7,12-dimethylbenz[a]anthracene (DMBA, O.19 μmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA, 1.6 nmol). Topical application of PPBa (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice (inhibitory ratio: IR = 56%, P &lt; 0.01 and 31%, P &lt; 0.005, respectively). PPBα exhibited potent anti-inflammatory activity in ICR mouse ears and moderate inhibitory activity toward TPA-induced superoxide (O 2 - ) generation in differentiated HL-60 cells. While CuPPBa, a synthetic copper complex of PPBα, exhibited higher anti-inflammatory activity than that of indomethacin, it showed little antioxidative effect against formation of lipid hydroperoxides (LOOHs) and malondialdehyde (MDA), suggesting that the antioxidative effect of PPBα might not be important for anti-inflammatory activity. These results imply that the active mechanism of PPBα for anti-tumor promotion might be partly involved in inhibition of TPA-induced inflammatory responses by suppressing leukocyte activation.

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  • Synthesis and biological activities of new conformationally restricted analogues of (-)-indolactam-V: Elucidation of the biologically active conformation of the tumor-promoting teleocidins

    K Irie, T Isaka, Y Iwata, Y Yanai, Y Nakamura, F Koizumi, H Ohigashi, PA Wender, Y Satomi, H Nishino

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   118 ( 44 )   10733 - 10743   1996年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    The tumor-promoting teleocidins and their core structure (-)-indolactam-V (1) exist in two stable conformers in solution at room temperature. The cis amide assumes a twist conformation while the trans amide exists in a sofa form. In order to identify the biologically active conformation of the teleocidins, we have synthesized new twist-restricted analogues 5a and 6 based on an aza-Claisen rearrangement of (-)-N-13-desmethyl-N-13-allylindolactam-V (3) and a sofa-restricted analogue, (-)-5-methylindolactam-V (22). The activities of these new compounds were evaluated in three in vitro bioassays associated with in vivo tumor-promoting activity: binding to the protein kinase C regulatory domain, induction of the Epstein-Barr virus early antigen, and stimulation of radioactive inorganic phosphate incorporation into phospholipids of HeLa cells. These three biological activities correlated well for each derivative, Twist-restricted analogues 5a and 6 showed significant activities in the three assays, comparable to 1 itself. In contrast, sofa-restricted 22 showed little activity related to tumor promotion. Introduction of a prenyl group into position 7 or 18 of 5a and 6 significantly enhanced the activity while sofa-restricted (-)-5-prenylindolactam-V (23) showed only very weak activity. These results indicate that the active conformation of the teleocidins and 1 is close to the twist form. This is the first evidence beating on the active conformation of the teleocidins based on conformationally restricted analogues with an intact indolactam skeleton and is in accord with conclusions reported for benzolactams, analogues without the pyrrole moiety. This study also describes the synthesis of hew biologically active compounds (26a, 26b, 28) based on inactive (+)-epiindolactam-V (24), involving a further application of the aza-Claisen rearrangement. Bridge formation between positions 5 and 13 of indolactam derivatives represents a particularly effective analogue design strategy, allowing for the remote control of the conformation of this ring system and for the introduction of a wide range of structural variations, as required for the development of new protein kinase C activators with high isozyme selectivity.

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  • 1’prime;-Acetoxychavicol acetate, a superoxide anion generation inhibitor, potently inhibits tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in ICR mouse skin

    Akira Murakami, Shin Ohura, Yoshimasa Nakamura, Koichi Koshimizu, Hajime Ohigashi

    Oncology (Switzerland)   53 ( 5 )   386 - 391   1996年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The anti-tumor-promoting activity of 1’-acetoxychavicol acetate (ACA) was examined in a two-stage carcinogenesis experiment in ICR mouse skin using 7,12-dimethylbenz[a]anthracene (0.19 μmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA; 1.6 nmol). Topical application of ACA (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice: inhibition ratios 90% (p &lt; 0.001) and 42% (p &lt; 0.005), respectively. ACA even at a dose equimolar to TPA (1.6 nmol) significantly reduced theaverage number of tumors per mouse: inhibitory ratio 44% (p &lt; 0.05). ACA potently inhibited TPA-induced superoxide (O2) generation in differentiated HL-60 cells (IC50 = 4.3μM) and suppressed the lipid hydroperoxide formation by42% (p &lt; 0.001) in the ethyl linoleate autoxidation test. © 1996 S. Karger AG, Basel.

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  • Photocytotoxicity of water-soluble fullerene derivatives

    Kazuhiro Irie, Yoshimasa Nakamura, Hajime Ohigashi, Hidetoshi Tokuyama, Shigeru Yamago, Eiichi Nakamura

    Bioscience, Biotechnology and Biochemistry   60 ( 8 )   1359 - 1361   1996年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    New water-soluble fullerene carboxylic acids (1 and 2) derived from C60 and C70 fullerenes, respectively, were examined for photocytotoxicity toward Raji cells (B lymphocyte). These compounds did not show any photocytotoxic effect even at 50μ m, while pheophorbide a showed significant photocytotoxicity at 0.5 μ m. Therefore, fullerene derivatives derived from C60 and C70 would not be practical agents for photodynamic therapy. © 1996, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.60.1359

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  • Identification of Pheophorbide a and Its Related Compounds as Possible Anti-tumor Promoters in the Leaves of Neptunia oleracea

    Yoshimasa Nakamura, Akira Murakami, Koichi Koshimizu, Hajime Ohigashi

    Bioscience, Biotechnology and Biochemistry   60 ( 6 )   1028 - 1030   1996年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Six chlorophyll-related compounds isolated from leaves of Neptunia oleracea (Leguminosae) inhibited the activation of tumor promoter-induced Epstein-Barr virus (EBV). Photo-irradiation of pheophorbide a, the major active constituent, did not enhance this inhibitory activity, and thus ruled out any photosensitizing effect playing the major role. © 1996, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.60.1028

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  • GLYCEROGLYCOLIPIDS FROM CITRUS HYSTRIX, A TRADITIONAL HERB IN THAILAND, POTENTLY INHIBIT THE TUMOR-PROMOTING ACTIVITY OF 12-O-TETRADECANOYLPHORBOL 13-ACETATE IN MOUSE SKIN

    A MURAKAMI, Y NAKAMURA, K KOSHIMIZU, H OHIGASHI

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   43 ( 10 )   2779 - 2783   1995年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    Two glyceroglycolipids were isolated from the leaves of Citrus hystrix (bitter orange), a traditional herb in Thailand. They were identified as 1,2-di-O-alpha-linolenoyl-3-O-beta-galactopyransyl-sn-glycerol (DLGG, I) and a mixture of two compounds, 1-O-alpha-linolenoyl-2-O-palmitoyl-3-O-beta-galactopyranosyl-sn-glycerol (2a) and its counterpart (2b) (LPGG, 2). Both lipids were potent inhibitors of tumor promoter-induced Epstein-Barr virus (EBV) activation. The IC50 values of 1 and 2 were strikingly lower than those of representative cancer preventive agents such as alpha-linolenic acid, beta-carotene, or (-)-epigallocatechin gallate. In a two-stage carcinogenesis experiment on ICR mouse skin with dimethylbenz[alpha]anthracene (DMBA) and 12-O-tetradecanoylphorbol 13-acetate (TPA), compound 1 exhibited anti-tumor-promoting activity even at a dose 10 times lower than that of alpha-linolenic acid. As some synthetic detergents or saponins were entirely inactive in the EBV activation inhibition test, detergency was suggested not to play a major role in the mode of inhibitory action in vivo. The inhibition of the arachidonic acid cascade may be involved in anti-tumor promotion since 1 inhibited TPA-induced edema formation in the anti-inflammation test using ICR mouse ears.

    DOI: 10.1021/jf00058a043

    Web of Science

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  • SYNTHESIS AND BIOLOGICAL-ACTIVITIES OF NEW CONFORMATIONALLY FIXED ANALOGS OF (-)-INDOLACTAM-V, THE CORE STRUCTURE OF TUMOR-PROMOTING TELEOCIDINS

    K IRIE, F KOIZUMI, Y IWATA, T ISHII, Y YANAI, Y NAKAMURA, H OHIGASHI, PA WENDER

    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   5 ( 5 )   453 - 458   1995年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    (-)-Indolactam-V(1) exists as two stable conformers, the twist and the sofa form, in solution at room temperature. 3-Aza-Cope rearrangement of (-)-N-13-desmethyl-N-13-allylindolactam-V (5) gave new conformationally restricted analogues (2a and 3) along with a normal rearrangement product (7). Both 2a and 3, whose fixed conformation was the twist form, showed significant biological activities related to tumor promotion.

    DOI: 10.1016/0960-894X(95)00047-W

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  • Possible anti-tumor promoting properties of edible plants from thailand, and identification of an active constituent, cardamonin, of boesenbergia pandurata

    Akira Murakami, Akira Kondo, Yoshimasa Nakamura, Hajime Ohigashi, Koichi Koshimizu

    Bioscience, Biotechnology and Biochemistry   57 ( 11 )   1971 - 1973   1993年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Possible anti-tumor promoting properties of a total of 40 methanol extracts from Thai edible plants, including 5 non-edible species, were estimated in vitro by an inhibition test of tumor promoter-induced Epstein-Barr virus (EBV) activation. Fourteen species (35% of the total) were found to be strongly active. In addition, cardamonin (2′,4′-dihydroxy-6′-methoxychalcone) was identified as a potently active constituent (IC50 = 3.1μM) of Boesenbergia pandurata (Zingiberaceae), suggesting it to be an effective anti-tumor promoter. © 1993 Taylor &amp
    Francis Group LLC.

    DOI: 10.1080/bbb.57.1971

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  • Possible anti-tumor promoting properties of edible plants from thailand, and identification of an active constituent, cardamonin, of boesenbergia pandurata

    Akira Murakami, Akira Kondo, Yoshimasa Nakamura, Hajime Ohigashi, Koichi Koshimizu

    Bioscience, Biotechnology and Biochemistry   57 ( 11 )   1971 - 1973   1993年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Possible anti-tumor promoting properties of a total of 40 methanol extracts from Thai edible plants, including 5 non-edible species, were estimated in vitro by an inhibition test of tumor promoter-induced Epstein-Barr virus (EBV) activation. Fourteen species (35% of the total) were found to be strongly active. In addition, cardamonin (2′,4′-dihydroxy-6′-methoxychalcone) was identified as a potently active constituent (IC50 = 3.1μM) of Boesenbergia pandurata (Zingiberaceae), suggesting it to be an effective anti-tumor promoter. © 1993 Taylor &amp
    Francis Group LLC.

    DOI: 10.1080/bbb.57.1971

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▼全件表示

書籍等出版物

  • Preparation of benzyl isothiocyanate-rich aqueous solution from mashed green papaya by distillation. Proceedings: International Symposium on Application of Advanced Technologies In Agriculture.

    Nakamura, Y., Murata, Y., Nakamura, T.( 担当: 単著)

    Van Hien University, Vietnam.  2020年12月 

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    担当ページ:pp. 243-247   記述言語:英語

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  • エッセンシャル食品化学

    中村宜督( 担当: 共編者(共編著者) ,  範囲: ビタミン)

    講談社  2018年12月 

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    記述言語:日本語

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  • エッセンシャル食品化学

    中村宜督, 榊???啓之, 室田佳恵子( 担当: 共編者(共編著者) ,  範囲: 天然色素)

    講談社  2018年12月 

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    記述言語:日本語

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  • エッセンシャル食品化学

    中村宜督( 担当: 共編者(共編著者) ,  範囲: 食品化学とは)

    講談社  2018年12月 

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    記述言語:日本語

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  • Visual栄養学テキスト 食べ物と健康I 食品学総論 食品成分と機能 第2章1−3 脂質

    中村宜督( 担当: 共著)

    中山書店  2018年1月 

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    担当ページ:45〜55   記述言語:日本語

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  • エッセンシャル食品化学 = Essential food chemistry

    中村 宜督, 榊原 啓之, 室田 佳恵子

    講談社  2018年  ( ISBN:9784065133415

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    記述言語:日本語

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  • 食品学総論 : 食品の成分と機能

    寺尾 純二, 村上 明, 津田 謹輔, 伏木 亨, 本田 佳子

    中山書店  2018年  ( ISBN:9784521742878

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    記述言語:日本語

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  • 栄養科学シリーズNEXT 基礎化学

    ( 担当: 共編者(共編著者))

    講談社  2010年3月 

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    担当ページ:iii, 28-66   記述言語:日本語

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  • 基礎化学

    辻 英明, 中村 宜督, 講談社サイエンティフィク

    講談社  2010年  ( ISBN:9784061553507

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    記述言語:日本語

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  • 基礎演習 産業生物化学

    中村宜督( 担当: 共著 ,  範囲: 機能性食品)

    岡山大学出版  2009年10月 

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    担当ページ:201   記述言語:日本語

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  • 食品機能性の科学

    *中村宜督( 範囲: ショウガ科植物に含まれるポリフェノール(ジンゲロール、クルクミン))

    産業技術サービスセンター  2008年4月 

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    担当ページ:pp.335-338  

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  • 食品機能性の科学

    *中村宜督( 範囲: ポリフェノールの害作用の可能性.)

    産業技術サービスセンター  2008年4月 

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    担当ページ:pp.319-323  

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  • 食品機能性の科学

    *中村宜督( 範囲: ゴマリグナン.)

    産業技術サービスセンター  2008年4月 

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    担当ページ:pp.331-334  

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  • 食品機能性の科学

    *中村宜督( 範囲: その他食品のポリフェノール.)

    産業技術サービスセンター  2008年4月 

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    担当ページ:pp.365-368  

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  • 食品機能性の科学

    *中村宜督( 範囲: アブラナ科野菜からのがん予防剤の開発.)

    産業技術サービスセンター  2008年4月 

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    担当ページ:pp.245-248  

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  • 機能性食品の事典

    *中村宜督, 大澤俊彦( 範囲: ゴマリグナン)

    朝倉書店  2007年8月 

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    担当ページ:pp.166-174.  

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  • 機能性食品の事典

    *中村宜督, 大澤俊彦( 範囲: クルクミン.)

    朝倉書店  2007年8月 

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    担当ページ:pp.175-181.  

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  • 機能性食品の安全性ガイドブック

    *中村宜督( 範囲: イソチオシアネート)

    サイエンスフォーラム,  2007年5月 

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    担当ページ:pp.406-410.  

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  • がん予防食品開発の新展開 ?予防医学におけるバイオマーカーの評価システム?

    *中村宜督( 範囲: パパイヤ.)

    シーエムシー出版, 東京  2005年4月 

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    担当ページ:pp. 302-310.  

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  • がん予防食品開発の新展開 ?予防医学におけるバイオマーカーの評価システム?

    *中村宜督( 範囲: がん抑制におけるアポトーシスの意義.)

    シーエムシー出版, 東京  2005年4月 

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    担当ページ:pp. 73-87.  

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  • Proceedings of the XII Biennial Meeting of the Society for Free Radical Research.

    *Nakamura, Y.( 範囲: Benzyl isothiocyanate as a multifunctional antioxidant: Implication for prevention against inflammation-related carcinogenesis.)

    Medimond, Bologna,  2004年4月 

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  • 第2回AOB研究会プロシーディング

    *中村宜督, 大東肇, 大澤俊彦( 範囲: 炎症部位での酸化的傷害に対する穀物発酵食品AOB (Antioxidant Biofactor) の抑制効果)

    2003年4月 

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    担当ページ:15-20  

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  • Food Factors in Health Promotion and Disease Prevention. ACS symposium series 851,

    *Nakamura, Y.( 範囲: Isothiocyanates as Inducers of Phase II Drug-metabolizing Enzyme.)

    ACS  2003年4月 

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    担当ページ:358-368  

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  • 財団法人漢方医薬研究振興財団 一般研究(平成13年度分)報告書

    *中村宜督( 範囲: 生薬に含まれる抗酸化性生体防御因子に関する研究)

    2003年4月 

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  • がん抑制の食品事典

    *中村宜督( 範囲: パパイヤ)

    法研  2003年4月 

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    担当ページ:118-121  

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  • Free Radicals in Food, Chemistry, Nutrition, and Health Effects. ACS symposium series 807

    Morimitsu, Y., *Nakamura, Y., Osawa, T., Uchida, K.( 範囲: Wasabi: A traditional Japanese food that contains as exceedingly potent glutathione S-transferase inducer for RL34 cells.)

    ACS  2002年4月 

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    担当ページ:301-309  

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  • Free Radicals in Food, Chemistry, Nutrition, and Health Effects. ACS symposium series 807

    Murakami, A. *Nakamura, Y., Koshimizu, K. Ohigashi, H.( 範囲: Suppressive effects of 1'-acetoxychavicol acetate on superoxide and nitric oxide in cell culture systems, and on hydrogen peroxide in mouse skin.)

    ACS  2002年4月 

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    担当ページ:329-341  

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  • 食と健康-情報のウラを読む-

    *中村宜督( 範囲: サプリメントの科学.)

    丸善  2002年4月 

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    担当ページ:31-51  

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  • ガン、脳卒中、心臓病を防ぐ食材事典

    *中村宜督( 範囲: パパイアは体内の解毒酵素を高め発ガン物質を無毒化する力が果物中最高)

    マキノ出版  2002年4月 

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    担当ページ:52-58  

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▼全件表示

MISC

  • 食品に見る 機能性成分のひみつ(最終回)ナッツの健康機能を担う脂溶性成分 α-リノレン酸&α-トコフェロール くるみ&アーモンド

    中村 宜督

    栄養と料理   87 ( 12 )   104 - 107   2021年12月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第41回)機能性脂質の一つ コレステロール低下作用に期待 エルゴステロール まいたけ

    中村 宜督

    栄養と料理   87 ( 11 )   104 - 107   2021年11月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第40回)コレステロール低下作用に期待! β-グルカン 大麦

    中村 宜督

    栄養と料理   87 ( 10 )   104 - 107   2021年10月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第39回)脂肪を燃やす働き!? カルニチン 羊肉

    中村 宜督

    栄養と料理   87 ( 9 )   104 - 107   2021年9月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第38回)脳機能を改善する!? 脳や心臓に多い資質 プラズマローゲン ホヤ

    中村 宜督

    栄養と料理   87 ( 8 )   104 - 107   2021年8月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第37回)トマトの赤色と健康機能を支える リコピン トマト

    中村 宜督

    栄養と料理   87 ( 7 )   104 - 107   2021年7月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第36回)脂肪からコロナまでの多彩な作用 茶カテキン 緑茶

    中村 宜督

    栄養と料理   87 ( 6 )   104 - 107   2021年6月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第35回)消化を促進する作用に注目!? アクチニジン キウイフルーツ

    中村 宜督

    栄養と料理   87 ( 5 )   104 - 107   2021年5月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る機能性成分のひみつ(第34回)スタミナのイメージにつながる成分 アリシンなどの含硫化合物 にんにく

    中村 宜督

    栄養と料理   87 ( 4 )   104 - 107   2021年4月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第33回)褐藻類に共通の機能性成分 食物繊維と色素成分 ひじき

    中村 宜督

    栄養と料理   87 ( 3 )   104 - 107   2021年3月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第32回)しょうがの辛味と健康機能を担う ジンゲロール しょうが

    中村 宜督

    栄養と料理   87 ( 2 )   104 - 107   2021年2月

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  • 食品に見る 機能性成分のひみつ(第31回)骨の健康に役立つ機能に注目 β-クリプトキサンチンほか みかん

    中村 宜督

    栄養と料理   87 ( 1 )   102 - 105   2021年1月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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    中村宜督

    現代化学   ( 601 )   2021年

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  • 食品に見る 機能性成分のひみつ(第30回)抗酸化作用を示すフラボノイド配糖体 ルチン そば

    中村 宜督

    栄養と料理   86 ( 12 )   104 - 107   2020年12月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第29回)心臓や血管の健康に貢献する多価不飽和脂肪酸 EPA(IPA)とDHA サンマ

    中村 宜督

    栄養と料理   86 ( 11 )   104 - 107   2020年11月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第28回)青汁の材料として有名な葉野菜 スルフォラファンとルテイン ケール

    中村 宜督

    栄養と料理   86 ( 10 )   104 - 107   2020年10月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第27回)ダイエットや認知症に有効? 中鎖脂肪酸 ココナツオイル

    中村 宜督

    栄養と料理   86 ( 9 )   104 - 107   2020年9月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • 食品に見る 機能性成分のひみつ(第26回)血圧が気になる人への機能性成分は? 酢酸とアミノ酸・有機酸 黒酢

    中村 宜督

    栄養と料理   86 ( 8 )   104 - 107   2020年8月

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  • 食品に見る 機能性成分のひみつ(第25回)大豆と納豆菌に由来する機能性成分 イソフラボン、γ-ポリグルタミン酸ほか 納豆

    中村 宜督

    栄養と料理   86 ( 7 )   104 - 107   2020年7月

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  • 食品に見る 機能性成分のひみつ(第24回)栄養がないといわれるきゅうりの成分 ビタミンCとククルビタシン きゅうり

    中村 宜督

    栄養と料理   86 ( 6 )   104 - 107   2020年6月

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  • 食品に見る 機能性成分のひみつ(第23回)米に含まれる多彩な機能性成分 γ(ガンマ)-オリザノール、イノシトールなど 米

    中村 宜督

    栄養と料理   86 ( 5 )   104 - 107   2020年5月

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  • Green leafy vegetables contain a compound which can fight cancer cells. 招待

    Public Relations Division, Okayama Univ, Yoshimasa Nakamura

    Okayama University Medical Research Updates   ( 77 )   1 - 3   2020年5月

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  • 食品に見る 機能性成分のひみつ(第22回)わかめ特有の機能性成分はない!? フコキサンチンなど わかめ

    中村 宜督

    栄養と料理   86 ( 4 )   104 - 107   2020年4月

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  • 食品に見る 機能性成分のひみつ(第21回)スーパーフードと呼ばれる理由は? アサイーポリフェノール アサイー

    中村 宜督

    栄養と料理   86 ( 3 )   104 - 107   2020年3月

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  • 食品に見る 機能性成分のひみつ(第20回)インフルエンザに効く? テアフラビン 紅茶

    中村 宜督

    栄養と料理   86 ( 2 )   102 - 105   2020年2月

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  • 食品に見る 機能性成分のひみつ(第19回)栄養ドリンク剤で有名な成分 タウリン タコ

    中村 宜督

    栄養と料理   86 ( 1 )   104 - 107   2020年1月

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  • 食品に見る 機能性成分のひみつ(第18回)毎日食べれば健康に!? プロシアニジンとカテキン類 りんご

    中村 宜督

    栄養と料理   85 ( 12 )   102 - 105   2019年12月

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  • 食品に見る機能性成分のひみつ(第17回)こんぶに含まれる水溶性食物繊維 フコイダンとアルギン酸 こんぶ

    中村 宜督

    栄養と料理   85 ( 11 )   102 - 105   2019年11月

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  • 食品に見る機能性成分のひみつ(第16回)鶏肉由来の抗疲労物質!? イミダゾールジペプチド 鶏肉

    中村 宜督

    栄養と料理   85 ( 10 )   102 - 105   2019年10月

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  • 食品に見る機能性成分のひみつ(第15回)皮に含まれるポリフェノール レスベラトロール ぶどう

    中村 宜督

    栄養と料理   85 ( 9 )   102 - 105   2019年9月

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  • 食品に見る機能性成分のひみつ(第14回)粘り成分はムチンではなかった! 糖たんぱく質とペクチン オクラ

    中村 宜督

    栄養と料理   85 ( 8 )   102 - 105   2019年8月

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  • 食品に見る 機能性成分のひみつ(第13回)とうがらし特有の辛味成分 カプサイシン とうがらし

    中村 宜督

    栄養と料理   85 ( 7 )   102 - 105   2019年7月

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  • 食品に見る 機能性成分のひみつ(第12回)解毒や成長にかかわるアミノ酸 オルニチン シジミ

    中村 宜督

    栄養と料理   85 ( 6 )   102 - 105   2019年6月

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  • 食品に見る 機能性成分のひみつ(第11回)ホモゲンチジン酸(アク成分)とアミノ酸(多彩な味成分) 竹の子

    中村 宜督

    栄養と料理   85 ( 5 )   102 - 105   2019年5月

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  • 食品に見る 機能性成分のひみつ(第10回)玉ねぎ独特の風味の源 イソアリインとその代謝物 玉ねぎ

    中村 宜督

    栄養と料理   85 ( 4 )   102 - 105   2019年4月

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  • 食品に見る 機能性成分のひみつ(第9回)海の赤い抗酸化成分 アスタキサンチン サケ

    中村 宜督

    栄養と料理   85 ( 3 )   102 - 105   2019年3月

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  • 食品に見る 機能性成分のひみつ(第8回)テオブロミン(苦味成分)とカカオポリフェノール(渋味成分) チョコレート・ココア

    中村 宜督

    栄養と料理   85 ( 2 )   102 - 105   2019年2月

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  • 食品に見る 機能性成分のひみつ(第7回)機能性成分のもととなる イソチオシアネート 大根

    中村 宜督

    栄養と料理   85 ( 1 )   102 - 105   2019年1月

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  • 食品に見る 機能性成分のひみつ(第6回)着色料や肝機能改善効果をねらった食品に利用 クルクミン ウコン

    中村 宜督

    栄養と料理   84 ( 12 )   104 - 107   2018年12月

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  • 食品に見る 機能性成分のひみつ(第5回)血圧改善作用ほか今後の研究が待たれる ゴマペプチドほか ごま

    中村 宜督

    栄養と料理   84 ( 11 )   104 - 107   2018年11月

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  • 食品に見る 機能性成分のひみつ(第4回)グアニル酸(うま味成分)と機能性糖類(レンチナンとトレハロース) しいたけ

    中村 宜督

    栄養と料理   84 ( 10 )   104 - 107   2018年10月

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  • 食品に見る機能性成分のひみつ(第3回)弱い女性ホルモン様作用 イソフラボンと多彩な機能性成分 大豆

    中村 宜督

    栄養と料理   84 ( 9 )   104 - 107   2018年9月

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  • 食品に見る機能性成分のひみつ(第2回)その種類が色を決める アントシアニン ブルーベリー

    中村 宜督

    栄養と料理   84 ( 8 )   104 - 107   2018年8月

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  • 食品に見る機能性成分のひみつ(第1回)オレイン酸&辛味成分 オリーブオイル

    中村 宜督

    栄養と料理   84 ( 7 )   104 - 107   2018年7月

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  • 抗酸化酵素の転写調節を可能にする食品成分 (特集 抗酸化食品の最前線研究)

    中村 宜督

    アグリバイオ = Agricultural biotechnology   2 ( 8 )   757 - 761   2018年7月

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    記述言語:日本語   出版者・発行元:北隆館  

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  • 食品因子isothiocyanate類が結合した炎症性サイトカインMIFの機能解析

    河合柚子, 橋詰力, 橋詰力, 眞鍋康子, 藤井宜晴, 中村宜督, 三好規之, 三好規之

    日本栄養・食糧学会大会講演要旨集   72nd   295 - 295   2018年4月

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  • 食品因子isothiocyanate類が結合した炎症性サイトカインMIFの機能解析

    河合 柚子, 橋詰 力, 眞鍋 康子, 藤井 宣晴, 中村 宜督, 三好 規之

    日本栄養・食糧学会大会講演要旨集   72回   295 - 295   2018年4月

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    記述言語:日本語   出版者・発行元:(公社)日本栄養・食糧学会  

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  • レプトスペリン,メチルシリンゲート及びその代謝物の細胞を用いた代謝機構の解明

    加藤陽二, 加藤陽二, 河合翔太, 室田佳恵子, 中村俊之, 中村宜督, 生城真一

    日本フードファクター学会学術集会講演要旨集   23rd   106   2018年

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  • モデル生物を用いた辛味成分イソチオシアネート標的遺伝子の同定と応用

    中村 宜督

    山陽放送学術文化財団リポート   ( 60 )   37 - 41   2016年9月

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    記述言語:日本語   出版者・発行元:山陽放送学術文化財団  

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  • Phenethyl isothiocyanateによるprotein tyrosine phosphatase1Bの阻害を介したレプチンシグナル活性化作用の解析

    八木美穂, 仲辻友紀子, 前田歩海, 三好規之, 中村宜督, 赤川貢

    日本香辛料研究会講演要旨集   31st   24‐25   2016年

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  • イソチオシアネートによるprotein tyrosine phosphatase 1Bの阻害を介したインスリンシグナリング活性化作用の解明

    仲辻友紀子, 前田歩海, 三好規之, 中村宜督, 赤川貢

    日本香辛料研究会講演要旨集   29th   23 - 24   2014年

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  • 女子高生が測定したキュウリによるトマトの抗酸化活性の低下作用

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    日本調理科学会大会研究発表要旨集   25 ( 0 )   2013年

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    記述言語:日本語   出版者・発行元:日本調理科学会  

    【目的】食品に含まれる抗酸化活性が注目される中、食品の相互作用による抗酸化活性の変化について研究を進めてきた。本研究では、キュウリによるトマトの抗酸化活性の低下作用を、抗酸化活性、ビタミンC含有量およびポリフェノール含有量の測定から解明を試みた。また、サラダの盛りつけを意識して接触状態での影響についても検討を行った。【方法】キュウリホモジネート、トマトホモジネートを1:1で懸濁し、抗酸化活性をDPPHラジカル捕捉活性法で、ビタミンC含有量をヒドラジン法で、ポリフェノール含有量をフォーリンチオカルト法で測定した。また、輪切りにしたキュウリをトマトの断面に接触させたのちに、トマトの抗酸化活性を測定した。【結果】キュウリホモジネート、トマトホモジネートを1:1で懸濁させることで、抗酸化活性およびポリフェノール含有量が、総和から期待される値よりも小さくなった。一方で、総ビタミンC含有量はキュウリとトマトの総和から期待される値とほぼ等しくなったが、酸化型ビタミンCの割合が大幅に増加していた。この結果から、キュウリに含まれるアスコルビン酸オキシダーゼがトマトの抗酸化活性の低下に大きく関与すると考えられた。また、トマトとキュウリを5分間の接触によって、トマトの抗酸化活性はわずかながら低下した。これらの結果から、キュウリにってトマトのアスコルビン酸の酸化が敏速に起こることが示唆された。

    DOI: 10.11402/ajscs.25.0.147.0

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  • がん予防食品因子イソチオシアネート結合タンパク質の網羅的探索法

    三好規之, 米持巧, 伴野勧, 中村宜督, 大島寛史

    日本生化学会大会(Web)   85th   3T11-05 (WEB ONLY) - 05   2012年12月

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    記述言語:日本語   出版者・発行元:(公社)日本生化学会  

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  • がん予防食品因子イソチオシアネート結合タンパク質の探索

    三好規之, 米持巧, 中村宜督, 大島寛史

    日本農芸化学会大会講演要旨集(Web)   2012   3J12P06 (WEB ONLY)   2012年3月

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  • 食品成分イソチオシアネートの標的タンパク質探索法の開発

    三好規之, 米持巧, 中村宜督, 大島寛史

    日本農芸化学会大会講演要旨集   2011   218   2011年3月

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  • 大腸がんHCT116細胞におけるがん予防成分イソチオシアネートの標的タンパク質探索

    米持巧, 三好規之, 中村宜督, 大島寛史

    日本農芸化学会大会講演要旨集   2011   218   2011年3月

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  • 3週間前に摂取した野菜の履歴が血中に(バイオミディア)

    中村 宜督

    生物工学会誌 : seibutsu-kogaku kaishi   89 ( 4 )   2011年

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    記述言語:日本語   出版者・発行元:日本生物工学会  

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  • イソチオシアネートとがん予防 (特集 がん予防と機能性食品)

    中村 宜督

    Functional food   4 ( 4 )   340 - 346   2011年

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    記述言語:日本語   出版者・発行元:フジメディカル出版  

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  • キウイ幼果抽出物は脂肪細胞分化と脂肪細胞へのグルコース取り込みを促進する

    齋藤武, 阿部大吾, 久保康隆, 中村宜督

    近畿中国四国農業研究成果情報(CD-ROM)   2010   2011年

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  • IQGAP(1) Compartmentalizes ROS-ependent VEGF Receptor 2 Activation and Signaling Linked to Endothelial Proliferation and Angiogenesis

    Masooma Razvi, Nihal Kaplan, Cristiana Caliceti, Yoshimasa Nakamura, Norifumi Urao, Ronald D. McKinney, Tohru Fukai, Masuko Ushio-Fukai

    CIRCULATION   122 ( 21 )   2010年11月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

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  • ROS-dependent Cysteine Sulfenic Acid Formation of IQGAP1 and ERK1/2 in VEGF-induced Endothelial Cell Proliferation and Migration

    Nihal Kaplan, Norifumi Urao, Masooma Razvi, Yoshimasa Nakamura, Leslie B. Poole, Tohru Fukai, Masuko Ushio-Fukai

    CIRCULATION   120 ( 18 )   S1085 - S1085   2009年11月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

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  • 食品に含まれる生体防御活性化物質

    中村 宜督

    ニューフードインダストリー   51 ( 9 )   29 - 39   2009年9月

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    記述言語:日本語   出版者・発行元:食品資材研究会  

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  • Detection of Cysteine Sulfenic Acid Formation: Role In Reactive Oxygen Species-dependent VEGF signaling Linked to Endothelial Migration

    Yoshimasa Nakamura, Nihal Kaplan, Leslie B. Poole, Tohru Fukai, Masuko Ushio-Fukai

    CIRCULATION   118 ( 18 )   S273 - S273   2008年10月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

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  • イリノイ大学シカゴ校での留学生活 : 血管新生のレドックス制御機構に関する研究

    中村 宜督

    化学と生物   46 ( 9 )   653 - 659   2008年9月

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    記述言語:日本語   出版者・発行元:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    筆者は,2006年10月から2008年3月までの約1年半の間,アメリカ・シカゴのイリノイ大学シカゴ校薬理学科にて,特別訪問研究員として研究活動を行なった.ここでは,Dr. Masuko Ushio-Fukai 研究室おける血管新生と活性酸素依存的シグナル伝達調節に関する研究と,シカゴでの留学生活を紹介したい.

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  • イソチオシアネートによるがん予防とその分子標的

    中村 宜督

    食品・食品添加物研究誌   213 ( 10 )   910 - 915   2008年

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    記述言語:日本語   出版者・発行元:FFIジャーナル編集委員会  

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  • Regulation of cell-cell adhesion and ROS-dependent VEGF signaling by protein tyrosine phosphatase 1B in endothelial cells

    Yoshimasa Nakamura, Nkolay A. Patrushev, Norifumi Urao, Hyoe Inomata, Ha Won Kim, Tohru Fukai, Masuko Ushio-Fukai

    CIRCULATION   116 ( 16 )   25 - 25   2007年10月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

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  • 酸化ストレスと情報伝達 (特集 ポリフェノールと健康)

    中村 宜督

    Food style 21   11 ( 10 )   47 - 49   2007年10月

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    記述言語:日本語   出版者・発行元:食品化学新聞社  

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  • 研究紹介 イソチオシアネートによるがんの化学予防の可能性

    中村 宜督

    岡山大学農学部学術報告   95   87 - 91   2006年2月

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    記述言語:日本語   出版者・発行元:岡山大学農学部  

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  • 基礎研究からの未来予想図 サプリメントはこれからどうなるか? (特集 サプリメントとどうつき合うか)

    中村 宜督

    栄養と料理   71 ( 12 )   82 - 86   2005年12月

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    記述言語:日本語   出版者・発行元:女子栄養大学出版部  

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  • イソチオシアネートの細胞死誘導に関与する内因性因子

    中村宜督, 渡辺悦子, 三好規之, 内田浩二, 大沢俊彦

    日本農芸化学会大会講演要旨集   2005   106   2005年3月

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    記述言語:日本語  

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  • イソチオシアネートによるがん予防の可能性 : 細胞増殖の選択的制御とその分子機構(第15回公開シンポジウム「食べ物によるがん予防戦略を考える : 生活環境中の植物成分の抗変異・抗発がん作用とそのメカニズム」)

    中村 宜督

    環境変異原研究   26 ( 3 )   253 - 258   2004年12月

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    記述言語:日本語   出版者・発行元:日本環境変異原学会  

    An important group of compounds that have a chemopreventive property is organosulfur compounds such as isothiocyanates. In the present study, we clarified the molecular mechanism underlying the relationship between benzyl isothiocyanate (BITC)-induced cell cycle arrest and apoptosis. The exposure of the cultured cells to BITC resulted in the inhibition of the G_2/M progression that coincided with apoptosis induction. An experiment using phase-specific synchronized cells demonstrated that the G_2/M phase-arrested cells are more sensitive to undergoing apoptotic stimulation by BITC than the cells in other phases. We identified phosphorylated Bcl-2 as a key molecule linking the p38 MAPK-dependent cell cycle arrest with JNK activation by BITC. We also found that BITC induced a cytotoxic effect more preferentially in the proliferating normal human colon epithelial cells than the quiescent cells. Moreover, down-regulation of p53 resulted in the enhancement of susceptibility to undergoing apoptotic stimulation by BITC. These findings suggested that p53 might play a negative regulating role in BITC-induced apoptosis. In conclusion, the results from this study provided biological evidence that BITC has a potential to induce apoptosis selectively in p53-mutated proliferating pre-cancerous cells.

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  • ベンジルイソチオシアネートによる細胞周期特異的なアポトーシス誘導機構

    中村宜督, 三好規之, 内田浩二, 大沢俊彦

    日本癌学会総会記事   63rd   521   2004年8月

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    記述言語:日本語  

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  • 野菜による解毒酵素誘導と生体内での抗酸化機能の向上 (特集 野菜と健康)

    中村 宜督

    食の科学   ( 317 )   17 - 24   2004年7月

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    記述言語:日本語   出版者・発行元:光琳  

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  • アピゲニンの細胞内プロオキシダント作用

    中村宜督, 難波きさ, 三好規之, 山田貴代, 内田浩二, 大沢俊彦

    日本農芸化学会大会講演要旨集   2004   77   2004年3月

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    記述言語:日本語  

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  • ベンジルイソチオシアナートの抗炎症作用

    中村宜督, 高林聡子, 三好規之, 大沢俊彦

    日本農芸化学会関西支部講演会講演要旨集   2003   20   2003年10月

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    記述言語:日本語  

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  • Benzyl isothiocyanateによる細胞周期特異的な細胞死に関する解析

    三好規之, 中村宜督, 内田浩二, 大沢俊彦

    日本農芸化学会大会講演要旨集   2003   62   2003年3月

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    記述言語:日本語  

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  • ショウガ科植物に含まれるアポトーシス誘導物質

    中村宜督, 上田泰弘, 三好規之, 阿部雅子, 小沢好夫, 内田浩二, 大沢俊彦

    日本農芸化学会大会講演要旨集   2003   62   2003年3月

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    記述言語:日本語  

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  • Wasabi: A Traditional Japanese Food That Contains an Exceedingly Potent Glutathione S-Transferase Inducer for RL34 Cells

    Yasujiro Morimitsu, Yasujiro Morimitsu, Y. Nakamura, Y. Nakamura, T. Osawa, K. Uchida

    ACS Symposium Series   807   301 - 309   2002年12月

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    In the course of our screening of edible plants on the induction of glutathione S-transferase (GST) activity in rat liver epithelial RL34 cells, 6-methylsulfinylhexyl isothiocyanate (MS-1TC) was isolated from wasabi (Wasabia japonica) as a potential inducer of GST. As a result from western blot analyses of GST expression induced by MS-ITC in RL34 cells, increasing of protein levels of both GST-Ya and GST-Yp were detected. And as a result from time dependent GSH level by MS-ITC, the GSH level in RL34 cells was initially decreased, then increased gradually to 1.8 times higher than its basal level after 12 hrs. By adding benzyl isothiocyanate (BITC) isolated from papaya instead of MSITC to the cells, the reactive oxygen intermediates (ROIs) detected by a fluorescence probe were increased immediately. From these data, we discuss the relation between levels of intracellular oxidation and the induction of GST.

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  • Benzyl isothiocyanateによる細胞周期停止機構の解析

    三好規之, 中村宜督, 内田浩二, 大沢俊彦

    生化学   74 ( 8 )   902 - 902   2002年8月

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    記述言語:日本語   出版者・発行元:(公社)日本生化学会  

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  • アピゲニンのアポトーシス誘導における活性酸素の役割

    難波きさ, 三好規之, 山田貴代, 大沢俊彦, 内田浩二, 中村宜督

    生化学   74 ( 8 )   1064 - 1064   2002年8月

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    記述言語:日本語   出版者・発行元:(公社)日本生化学会  

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  • 植物性食品因子アピゲニンによる細胞死誘導機構の解析

    中村宜督, 三好規之, 内田浩二, 大沢俊彦

    日本癌学会総会記事   61st   478   2002年8月

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    記述言語:日本語  

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  • フラボノイドによる細胞死誘導と活性酸素の関与

    中村宜督, 山田貴代, 三好規之, 内田浩二, 大沢俊彦

    日本農芸化学会大会講演要旨集   2002   245   2002年3月

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    記述言語:日本語  

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  • 食品因子benzyl isothiocyanateによるredox感受性細胞死の誘導機構

    三好規之, 中村宜督, 内田浩二, 大沢俊彦

    日本農芸化学会大会講演要旨集   2002   245   2002年3月

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    記述言語:日本語  

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  • Role of p38 mitogen-activated protein kinase in the 4-hydroxy-2-nonenal-induced cyclooxygenase-2 expression

    T Kumagai, Y Nakamura, T Osawa, K Uchida

    FREE RADICAL BIOLOGY AND MEDICINE   33   S67 - S68   2002年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

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  • Food phytochemicals suppressing free-radical generation: Implications for cancer chemoprevention.

    A Murakami, Y Nakamura, K Koshimizu, H Ohigashi

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   219   U27 - U27   2000年3月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER CHEMICAL SOC  

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  • 活性酸素による制がんの可能性

    中村 宜督

    バイオサイエンスとインダストリー = Bioscience & industry   57 ( 8 )   40 - 41   1999年8月

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    記述言語:日本語  

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  • 抗腫瘍性プロスタグランジン類による遺伝子発現誘導

    川本 善之, 内藤 裕子, 鳥居 恭好, 中村 宜督, 大東 肇, 今川 正良, 佐藤 公彦, 大澤 俊彦, 内田 浩二

    生化学   71 ( 8 )   882 - 882   1999年8月

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    記述言語:日本語   出版者・発行元:(公社)日本生化学会  

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  • Auraptene, an Alkyloxylated Coumarin from Citrus natsudaidai HAYATA, Inhibits Mouse Skin Tumor Promotion and Rat Colonie Aberrant Crypt Foci Formation

    Akira Murakami, Wataru Kuki, Yasuo Takahashi, Hiroshi Yonei, Takuji Tanaka, Hiroki Makita, Keiji Wada, Naomi Ueda, Masanobu Haga, Yoshimasa Nakamura, Yoshimi Ohto, Oe Kyung Kim, Hajime Ohigashi, Koichi Koshimizu, Koichi Koshimizu

    ACS Symposium Series   701   86 - 95   1998年12月

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    Auraptene, a coumarin-related compound, has been isolated from the cold-pressed oil of natsumikan (Citrus natsudaidai HAYATA), as an inhibitor of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced Epstein-Barr virus (EBV) activation in Raji cells. In a two-stage carcinogenesis experiment with 1.6 nmol of TPA and 0.19 μmol of 7,12-dimethyl benz[a]anthracene (DMBA) in ICR mouse skin, topical application of 160 nmol of auraptene significantly reduced tumor incidence and the number of tumors per mouse by 27% and 23%, respectively. Auraptene at 50 μM almost completely suppressed TPA-induced superoxide (O 2 - )and hydroperoxide (ROOH) generation in differentiated HL-60 cells as well as lypopolysaccharide (LPS)/interferon-γ(IFN-γ)-induced nitric oxide (NO) generation in RAW 264.7 cells. Dietary feeding of auraptene at a dose of 100 or 500 ppm inhibited azoxymethane (AOM)-induced rat colonic aberrant crypt foci (ACF) formation in a dose-dependent manner. Oral administration of auraptene (50 - 200 mg/kg body wt.) clearly enhanced the glutathione S-transferase (GST) activity in mouse liver, suggesting that auraptene is an effective chemopreventer in both tumor initiation and promotion phases.

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  • フェノール性抗酸化剤の発がん抑制に対する二面性

    中村 宜督

    化学と生物   36 ( 11 )   728 - 729   1998年11月

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    記述言語:日本語   出版者・発行元:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI: 10.1271/kagakutoseibutsu1962.36.728

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  • Cellular response to phenolic antioxidants

    K Uchida, Y Naito, Y Torii, Y Nakamura, T Kondo, T Osawa

    FREE RADICAL BIOLOGY AND MEDICINE   25   S53 - S53   1998年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

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  • Auraptene, a citrus coumarin, inhibits 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion in ICR mouse skin.

    A Murakami, W Kuki, Y Takahashi, H Yonei, Y Nakamura, Y Ohto, H Ohigashi, K Koshimizu

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   213   154 - AGFD   1997年4月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER CHEMICAL SOC  

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▼全件表示

講演・口頭発表等

  • Preparation of benzyl isothiocyanate-rich aqueous solution from mashed green papaya by distillation.

    Nakamura, Y. Murata, Y., Nakamura, T.

    International Symposium on Application of Advanced Technologies In Agriculture. 

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    開催年月日: 2020年10月2日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

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  • ベンジルイソチオシアネートは脂肪細胞によるインスリンシグナル伝達を強化する

    中田小百合、Ying Liang、中村俊之、宗正晋太郎、村田芳行、中村宜督

    岡山大学次世代研究拠点シンポジウム2020 

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    開催年月日: 2020年1月10日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:岡山  

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  • 食品由来ポリフェノール類がアセトアルデヒドの皮膚表皮細胞への毒性に与える影響

    明神匠、Xu Wensi、宗正晋太郎、中村俊之、村田芳行、中村宜督

    岡山大学次世代研究拠点シンポジウム2020 

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    開催年月日: 2020年1月10日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:岡山  

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  • The distinguishable absorption and metabolism of quercetin coexisted with other food components

    Rikito Mitsuzane, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

    The 7th International Conference on Food Factors 

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    開催年月日: 2019年12月3日

    記述言語:英語   会議種別:ポスター発表  

    開催地:神戸  

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  • Characterization of the monoclonal antibody to α-carboxyethyl hydroxychroman

    Daiki Moriya, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

    The 7th International Conference on Food Factors 

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    開催年月日: 2019年12月3日

    記述言語:英語   会議種別:ポスター発表  

    開催地:神戸  

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  • Benzyl isothiocyanate enhances insulin signaling in adipocytes

    Sayuri Nakata, Ying Liang, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

    The 7th International Conference on Food Factors 

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    開催年月日: 2019年12月3日

    記述言語:英語   会議種別:ポスター発表  

    開催地:神戸  

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  • Difference in antioxidant capacity of rice extracts among rice categories and degree of milling and processing

    Hongyan Wu, Miho Hirooka, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

    The 7th International Conference on Food Factors 

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    開催年月日: 2019年12月2日

    記述言語:英語   会議種別:ポスター発表  

    開催地:神戸  

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  • Improvement of the benzyl isothiocyanate stability in aqueous solutions

    Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura

    The 7th International Conference on Food Factors 

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    開催年月日: 2019年12月2日

    記述言語:英語   会議種別:ポスター発表  

    開催地:神戸  

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  • Inhibition of drug resistance mechanisms ameliorates the benzyl isothiocyanate-induced antiproliferation in human colorectal cancer cells 招待

    Yoshimasa Nakamura

    The 7th International Conference on Food Factors 

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    開催年月日: 2019年12月2日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:神戸  

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  • Modulating effects of polyphenols on the acetaldehyde-induced cytotoxicity in human keratinocyte HaCaT cells

    Takumi Myojin, Wensi Xu, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura

    The 9th International Conference on Polyphenols and Health 

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    開催年月日: 2019年11月30日

    記述言語:英語   会議種別:ポスター発表  

    開催地:神戸  

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  • Identification of the target proteins of quercetin glycoside catabolites as cytoprotective agent

    Kouki Morita, Sayaka Nakashima, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

    The 9th International Conference on Polyphenols and Health 

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    開催年月日: 2019年11月29日

    記述言語:英語   会議種別:ポスター発表  

    開催地:神戸  

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  • リンゴ酸誘導気孔閉口におけるシグナル分子の同定

    三俣好令、宗正晋太郎、中村俊之、中村宜督、村田芳行

    日本農芸化学会中四国支部第54回講演会 

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    開催年月日: 2019年6月1日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岡山  

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  • Mechanisms of Reactive Carbonyl Species-Mediated Stomatal Closure in Arabidopsis Guard Cells

    Mohammad Saidur Rhaman、宗正晋太郎、中村宜督、村田芳行

    日本農芸化学会中四国支部第54回講演会 

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    開催年月日: 2019年6月1日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:岡山  

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  • 食品由来ポリフェノール類がアセトアルデヒドの皮膚表皮細胞への毒性に与える影響

    明神匠、Xu Wensi、宗正晋太郎、中村俊之、村田芳行、中村宜督

    日本農芸化学会中四国支部第54回講演会 

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    開催年月日: 2019年6月1日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岡山  

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  • 食品成分の化学構造か