研究者詳細 - 沢　禎彦

論文 - 沢　禎彦

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[A case of recrudescent groove pancreatitis, which was in remission by proximal gastrectomy with vagotomy for gastric cancer].

Hideki Aragane, Hideki Fujii, Takaharu You, Atuhiro Morita, Morimichi Miyazaki, Kiyoshi Morita, Tohru Ohkawara, Shinji Fukumitu, Yoshihiko Sawa, Yasuo Ohkawara

Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology 103 ( 5 ) 537 - 42 2006年5月

記述言語：日本語掲載種別：研究論文（学術雑誌）

This report describes our experience with a 60 year old male who suffered from a recrudescence of groove pancreatitis. He had been treated by conservative medication therapy by proton pump inhibitor used for therapy of duodenal ulcer, and was in remission. During a follow-up one year later, endoscopy revealed gastric cancer, for which a proximal gastrectomy and vagotomy were performed. The patient continues to remain in remission for the groove pancreatitis. Our experience with the clinical course of this disease, in which treatment for duodenal ulcer was used effectively, offers new insights into the progression and therapy of groove pancreatitis.

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Intracellular distribution of desmoplakin in human odontoblasts 査読国際誌

Y Sawa, S Kuroshima, Y Yamaoka, S Yoshida

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 53 ( 9 ) 1099 - 1108 2005年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：HISTOCHEMICAL SOC INC

Coexpression of desmosomal proteins and vimentin has been reported in a specific mesenchymal phenotype. This study investigated the expression of vimentin-binding desmosomal proteins in human dental pulp fibroblasts (DPF) and odontoblasts. The dental pulp has no cells expressing desmocollin (DSC) 1-3, desmoglein (DSG) 1-3, junction plakoglobin (JUP), or desmoplakin (DPK) 1 and 2 except for odontoblasts expressing DPK. A confocal image by laser-scanning microscopy demonstrated the diffuse distribution of DPK in the cytoplasm throughout the odontoblast processes. In culture, the mRNA expression of JUP and DPK1, but not DSC1-3 and DSG1-3, was detected in all DPF clones tested and also in odontoblast-like cells (OB) expressing osteocalcin and dentin sialophosphoprotein mRNAs established in the differentiation medium. The DPF having the potential to differentiate into OB expressed vimentin, but not DPK before culturing in the differentiation medium, whereas OB expressed vimentin-binding DPK1. These results suggest that DPF usually expresses DPK1 mRNA, and that the DPK1 production and the bonding of vimentin to DPK1 occur in DPF with the differentiation into odontoblasts.

DOI： 10.1369/jhc.4A6525.2005

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Expression of cys-cys chemokine ligand 21 on human gingival lymphatic vessels 査読国際誌

S Kuroshima, Y Sawa, Y Yamaoka, K Notani, S Yoshida, N Inoue

TISSUE & CELL 36 ( 2 ) 121 - 127 2004年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CHURCHILL LIVINGSTONE

The cys-cys (C-C) chemokine ligand 21 is a member of the C-C chemokines that constitute a group of heparin-binding cytokines with a pattern of four or six conserved cysteines. The CCL21 is known to be expressed in secondary lymphoid tissues, however it has rarely been reported for the expression on peripheral lymphatic vessels in somatic tissue. Here we investigated the expression of CCL21 on lymphatic vessels identified by anti-desmoplakin in uninflamed and inflamed human gingiva. In uninflamed tissue the expression of CCL21 was detected on lymphatic vessels in gingiva. In uninflamed gingiva the expression of CCL21 was detected on all lymphatic capillaries of the mucosal connective tissue papillae. There were two types of collecting lymphatic vessels in the lamina propria mucosae expressing CCL21 strongly or very weakly. In inflamed gingiva no expression of CCL21 was detected on lymphatic vessels. In all tissue sections no blood vessels expressing CCL21 were observed. These results may suggest that the expression of CCL21 is predominantly induced in the peripheral lymphatic endothelium of the uninflamed mucosal microcirculation, and that under inflamed conditions a reduction of CCL21 occurs in lymphatic endothelium. (C) 2003 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tice.2003.10.004

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Reduction of alkaline phosphatase activity in aged human osteogenic periodontal ligament fibroblasts exhibiting short telomeres 査読国際誌

Y Sawa, Y Yamaoka, S Kuroshima, S Yoshida

CELL AND TISSUE RESEARCH 315 ( 3 ) 331 - 337 2004年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPRINGER-VERLAG

The osteogenic cell type of human periodontal ligament fibroblasts (PDLF) undergoes senescence at finite population doubling numbers unrelated to donor ages. This study investigated telomere lengths of osteogenic PDLF from differently aged donors and alterations of the osteoblast-like properties in the aged PDLF with short telomeres. Telomere lengths of osteogenic PDLF were biased towards long or short among all 15- to 51-year-old individuals, and did not show a normal distribution by Pearson's test or a correlation to donor age by simple regression analysis. In osteogenic PDLF, senescence-associated beta-galactosidase was expressed in 78.5% of cells in the clones with short telomeres (mean 3.02 kbp), and in 9.4% of cells in the clones with long telomeres (mean 13.06 kbp). These results suggest that human periodontium comprises aged osteogenic PDLF without correlation to age. Osteogenic PDLF with long telomeres strongly expressed alkaline phosphatase (ALPase) activity whereas cells with short telomeres expressed ALPase activity to a weaker extent. Total activity of ALPase in the clones of osteogenic PDLF with long telomeres was significantly higher than that in the clones with short telomeres. The produced amounts of both osteopontin and osteocalcin in the clones of osteogenic PDLF with long telomeres were slightly but statistically significantly smaller than those in the clones with short telomeres. These findings suggest that aged osteogenic PDLF reduce the expression of ALPase activity but that there is not a critical alteration in bone-associated protein production. Aged osteogenic PDLF may impair the ability to induce ALPase-dependent calcification.

DOI： 10.1007/s00441-003-0837-7

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Expression of Toll-like receptors 2 and 4 on human intestinal lymphatic vessels 査読国際誌

Microvascular Research 67 ( 1 ) 90 - 95 2004年1月

Plastic casts and confocal laser scanning microscopy applied to the observation of enamel tubules in the red Kangaroo (Macropus rufus).

Masatsugu Suzuki, Natsumi Ushijima, Ayako Kohno, Yoshihiko Sawa, Shigemitsu Yoshida, Mitsuo Sekikawa, Noriyuki Ohtaishi

Anatomical science international 78 ( 1 ) 53 - 61 2003年3月

記述言語：英語掲載種別：研究論文（学術雑誌）

Scanning electron microscopy for plastic casts and confocal laser scanning microscopy for Villanueva bone-stained ground sections were used together to observe enamel tubules in red kangaroo molars. Although the tubular structures such as terminals, bends, expansions, splits, divergences and rejoinings in this species were within the variations of marsupial species, their morphological characteristics were demonstrated with extremely clear and persuasive images. Thus, the combined observations of plastic casts by scanning electron microscopy and Villanueva bone-stain sections by confocal laser scanning microscopy were found to be of value for the investigation of enamel tubules and tubular structures in other hard tissues.

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Production of colony-stimulating factor in human dental pulp fibroblasts 査読

Y Sawa, Y Horie, Y Yamaoka, N Ebata, T Kim, S Yoshida

JOURNAL OF DENTAL RESEARCH 82 ( 2 ) 96 - 100 2003年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R

Class II major histocompatilibity complex (MHC)-expressing cells are usually distributed in dental pulp, and it was postulated that the colony-stimulating factor (CSF) derived from dental pulp fibroblasts contributes to the migration of class II MHC-expressing cells into pulp tissue. This study aimed to investigate the CSF production of human dental pulp fibroblasts. In pulp tissue sections, granulocyte (G)-CSF was detected from normal teeth, while G-CSF, macrophage (M)-CSF, and granulocyte-macrophage (GM)-CSF were detected from teeth with dentinal caries. In cultured dental pulp fibroblasts, G-CSF was detected by immunostaining, immunoprecipitation, and ELISA, and mRNAs of G-CSF, M-CSF, and GM-CSF were detected by RT-PCR. The dental pulp fibroblasts cultured with TNF-alpha were found to increase the G-CSF expression and to produce M-CSF and GM-CSF. These findings suggest that dental pulp fibroblasts usually produce G-CSF. In the presence of TNF-alpha, dental pulp fibroblast express M-CSF and GM-CSF.

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Cultured periodontal ligament fibroblasts express diverse connexins 査読国際誌

Y Yamaoka, Y Sawa, N Ebata, N Ibuki, S Yoshida

TISSUE & CELL 34 ( 6 ) 375 - 380 2002年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CHURCHILL LIVINGSTONE

Recent studies have suggested multiple functions of periodontal ligament fibroblasts (PDLFs) which may relate to the permeability of gap junctions composed of various types of connexins (Cxs). At present, 15 types of Cxs are known to exist, and six of their antibodies, anti-Cx26, Cx32, Cx37, Cx40, Cx43, and Cx45 are commercially available. This study aims to examine which types of Cxs are expressed in cultured PDLFs by an immunohistochemical method, western blotting, and RT-PCR.
The study confirmed the expressions of Cx32, Cx40, Cx43, and Cx45 in PDLFs, while Cx26 and Cx37 were not detected. Considering previous reports, Cx32 may relate to the secretory function, and Cx40 and Cx45 to the contractile function of PDLFs, however, a function for Cx43 has not been specified. In the immunohistochemical examination, different localizations of Cx40/43 and Cx32/45 were established. The former were observed punctately, suggesting that a large part of Cx40/43 may exist in the cell membrane and construct gap junctions. In contrast, the latter were observed uniformly in all the cells, indicating that they are present both in the cell membrane and in the cytoplasm of the cells. (C) 2002 Elsevier Science Ltd. All rights reserved.

DOI： 10.1016/S0040-8166(02)00038-1

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Different expressions of connexin 43 and 32 in the fibroblasts of human dental pulp 査読

N Ibuki, Y Yamaoka, Y Sawa, T Kawasaki, S Yoshida

TISSUE & CELL 34 ( 3 ) 170 - 176 2002年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CHURCHILL LIVINGSTONE

The expression and localization of gap junctional proteins connexin (Cx) 26, 32, and 43 was examined in human dental pulp. Dental pulp tissues were obtained from human third molars immediately after extraction. Some pulp tissues were used for cell culture, and the rest for histological observations. Immunostaining for cultured dental pulp fibroblasts (DPFs) showed that Cx32 and 43 were expressed in human DPFs, and proteins corresponding to 27 (Cx32) and 43 kDa (Cx43) were identified by Western blot analysis. Immunostaining for tissue sections showed that the expression of Cx32 and 43 was observed in the entire region of the pulp and further strong expression of Cx32 was established beneath the cell-rich zone. Considering the close relationship between Cx types and cell functions, the results indicate that DPFs beneath the cell-rich zone may have specific, Cx32-related functions. The cell rich zone is thought to contain progenitor odontoblasts that can be induced to differentiate into mature odontoblasts in response to wounding. Therefore, it may be hypothesized that DPFs just beneath the cell-rich zone produce proteins and induce odontoblast differentiation from the cells in the cell-rich zone. (C) 2002 Elsevier Science Ltd. All rights reserved.

DOI： 10.1016/S0040-8166(02)00028-9

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Immunoelectron microscopic study of PECAM-1 expression on lymphatic endothelium of the human tongue 査読

N Ebata, Y Sawa, Y Nodasaka, Y Yamaoka, S Yoshida, Y Totsuka

TISSUE & CELL 33 ( 3 ) 211 - 218 2001年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CHURCHILL LIVINGSTONE

The expression of platelet-endothelial cell adhesion molecule-1 (PECAM-1) on lymphatic and blood vessels of the human tongue was examined with fluorescence and transmission electron microscopy (TEM), The study used anti-desmoplakins antiserum for light microscopic identification of the lymphatic vessels, plus a pre-embedding immunogold electron microscopic technique for TEM observations. Before making TEM observations, cryostat serial sections were immunostained with anti-desmoplakins or anti-PECAM-l and then embedded. Semithin sections from each cryostat section were photographed under a light microscope and compared in order to identify the lymphatic vessels expressing PECAM-1. In fluorescence microscopy, PECAM-1 expression on lymphatic vessels was weaker than that on blood vessels, TEM observations showed that PECAM-1 expression on the blood vessels was observed only on the luminal surface of the endothelium. In lymphatic vessels, PECAM-1 expression was found both on the luminal and abluminal surfaces of the endothelium. The density of the PECAM-1 reaction products was lower in lymphatic vessels than in blood vessels. The density of PECAM-1 reaction products on the luminal surface of lymphatic vessels was higher than on the abluminal surfaces. The results suggest that blood vessels are more active than lymphatic vessels in leukocyte migration. The expression of PECAM-1 on the abluminal surface of lymphatic endothelium may allow leukocytes to adhere to the endothelium and interact in their migration from tissue into lymphatic vessels. (C) 2001 Harcourt Publishers Ltd.

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Desmoplakin as a specific marker of lymphatic vessels 査読

N Ebata, Y Nodasaka, Y Sawa, Y Yamaoka, S Makino, Y Totsuka, S Yoshida

MICROVASCULAR RESEARCH 61 ( 1 ) 40 - 48 2001年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE

The usefulness of immunostaining with anti-desmoplakin antibody for light microscopic identification of lymphatic vessels was examined in cryostat sections of the human tongue. The results were compared with laminin, 5'-nucleotidase (5'-Nase), and factor VIII staining. Immunoelectron microscopic observation was also performed to confirm that the vessels reacting with anti-desmoplakin were lymphatic vessels. Under the immunoelectron microscopic, the vessels reacting with anti-desmoplakin showed ultrastructural features characteristic of lymphatic vessels: thin endothelial walls, no or incomplete basal lamina, open junctions, and overlapping endothelium. In general, lymphatic vessels identified by anti-desmoplakin reacted strongly with 5'-Nase, but showed weak or no reactivity with anti-laminin and anti-factor VIII. Blood vessels showed no reactivity with anti-desmoplakin, but reacted strongly with anti-laminin and anti-factor VIII. However, some blood and lymphatic vessels showed intermediate reactivity with anti-laminin, anti-factor VIII, and 5'-Nase. It was difficult to identify these as blood or lymphatic vessels only by the reactivity differences. The results indicate that anti-desmoplakin antibody specifically distinguishes lymphatic vessels and is useful for studying the fine distribution of lymphatic vessels under light microscopy. (C) 2000 Academic Press.

DOI： 10.1006/mvre.2000.2280

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Double expressions of connexin 43 and 32 in human periodontal ligament fibroblasts 査読

Y Yamaoka, Y Sawa, N Ebata, N Ibuki, S Yoshida, T Kawasaki

TISSUE & CELL 32 ( 4 ) 328 - 335 2000年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CHURCHILL LIVINGSTONE

The expressions of connexin 43 and 32 in cultured and intact human periodontal ligament fibroblasts (PDLFs) were examined using immunohistochemical methods, and western blot analysis was conducted with anti-connexin 43 and 32 in cultured PDLFs. The PDLFs both in cultured cells and tissue sections reacted with anti-connexin 43 and 32, and western blot analysis showed bands of approximately 43 kD and 27 kD reacted with anti-connexin 43 and 32 respectively, suggesting the existence of gap junctions in human PDLFs, In cultured PDLFs there were no reaction products of connexin 43 when the cells were not in contact with adjacent cells, but reaction products were increasingly observed with increases in cell-cell contacts. Different from connexin 43, the reaction products of connexin 32 were found in the cytoplasm, regardless of whether the cells were or were not in contact with adjacent cells. Further, the reaction activity of connexin 32 varied among PDLFs; some were strong, some moderate, and some weak. The expressions of connexin 43 and 32 in human PDLFs are suggested to be related to the regulation of two different functions of the PDLFs. (C) 2000 Harcourt Publishers Ltd.

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Desmosomal proteins in cultured and intact human periodontal ligament fibroblasts 査読

Y Yamaoka, Y Sawa, N Ebata, S Yoshida, T Kawasaki

TISSUE & CELL 31 ( 6 ) 605 - 609 1999年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CHURCHILL LIVINGSTONE

This study examined the kinds of desmosomal proteins in the human periodontal ligament fibroblasts (PDLFs), The PDLFs obtained from young and older patients were cultured and the amounts of desmosomal proteins were measured by ELISA with antibodies to desmoplakins, desmogleins, and desmocollins. Cultured cells and tissue sections of the human periodontal ligament were immunostained with the same antibodies. Expression of desmosomal proteins in the PDLFs was clearly demonstrated both by ELISA and the immunohistochemical studies, suggesting the existence of desmosome-like junctions in the PDLFs, The junctions are considered to protect gap junctions in the PDLFs against cell transformation caused by cell contraction, which may relate to tooth eruption and repair of periodontal tissue, and/or strong occlusal forces, Statistically significant differences (P<0.0001) in the expression of desmoplakins and desmogleins between younger and older patients were observed in this study, (C) 1999 Harcourt Publishers Ltd.

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Transcriptional activation of mRNA of intercellular adhesion molecule 1 and induction of its cell surface expression in normal human gingival fibroblasts by Mycoplasma salivarium and Mycoplasma fermentans 査読

L Dong, KI Shibata, Y Sawa, A Hasebe, Y Yamaoka, S Yoshida, T Watanabe

INFECTION AND IMMUNITY 67 ( 6 ) 3061 - 3065 1999年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER SOC MICROBIOLOGY

Lipoproteins in the cell membranes of both Mycoplasma salivarium and Mycoplasma fermentans were demonstrated to trigger the transcription of intercellular adhesion molecule-1 mRNA in normal fibroblasts isolated from human gingival tissue and to induce its cell surface expression by a mechanism distinct from that of Escherichia call lipopolysaccharide. The lipid moiety of the lipoproteins was suggested to play a key role in the expression of the activity.

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Lymphatic endothelium of the human tongue expresses multiple leukocyte adhesion molecules 査読

N Ebata, Y Sawa, Y Ashikaga, Y Yamaoka, M Suzuki, Totsuka, V, S Yoshida

TISSUE & CELL 31 ( 1 ) 34 - 38 1999年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CHURCHILL LIVINGSTONE

The expression of leukocyte adhesion molecules on lymphatic vessels of the human tongue was examined using histochemical and immunohistochemical methods. Three different types of lymphatic vessels were distinguished: type I vessels expressed intercellular adhesion molecule-1 (ICAM-1), platelet-endothelial cell adhesion molecule-1 (PECAM-1), and endothelial cell-selectin (ELAM-1); type II vessels expressed ICAM-1 and PECAM-1; and type III vessels expressed PECAM-1 only, The lymphatic vessels located very close to the oral epithelium (lymphatic capillaries) and the other lymphatic vessels near the oral epithelium were type I. The lymphatic vessels in the submucosal connective tissue (collecting lymphatic vessels) were type II and type III. The results suggest that there may be functional differences in the lymphatic endothelium, where lymphatic capillaries are more active than collecting lymphatic vessels in lymphocyte migration from tissue into the lymphatic vessels.

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