Faculty Profiles - TAMURA Takashi

A new selenoprotein from human lung adenocarcinoma cells: Purification, properties, and thioredoxin reductase activity

Takashi Tamura, Thressa C. Stadtman

Proceedings of the National Academy of Sciences of the United States of America 93 ( 3 ) 1006 - 1011 1996.2

Language：English

DOI： 10.1073/pnas.93.3.1006

Antimicrobial activity against methicillin-resistant Staphylococcus aureus in the culture broth of Enterococcus faecalis TH 10, an isolate from Malaysian fermentation food, Temph

OHHIRA Iichiro, TAMURA Takashi, FUJII Nozomi, INAGAKI Kenji, TANAKA Hidehiko

JAPANESE JOURNAL OF DAIRY AND FOOD SCIENCE 45(4), 93-96 ( 4 ) A - 93-A-96 1996

Language：Japanese Publisher：Japanese Dairy Science Association

Bioassay-directed screening for antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) led us to the culture broth of Enterococcus faecalis TH 10, which was isolated from a Malaysian fermentation food, Temph. The anti-MRSA component was readily extracted with ethyl acetate from the culture broth at pH 3, and the extract retained the activity when incubated overnight with various proteolytic enzymes. In addition, the extract showed poor antimicrobial activity against various lactic acid bacteria, including Enterococcus faecalis. These properties strongly suggest that the active compound is distinct from bacteriocin, an extracellularly released peptide or protein which shows potent activity against species closely related to the bacteriocin-producing strain. E. faecalis has been reported to produce bacteriocin which has strong hemolytic activity on mammalian erythrocytes, but the active component of the TH 10 strain did not mediate the lysis of rabbit and human erythrocytes.

DOI： 10.11465/milkscience.45.A-93

CiNii Article

CiNii Books

Purification and Characterization of Xylanase from Acidobacterium capsulatum.

Other Link： https://jlc.jst.go.jp/DN/JLC/20016775976?from=CiNii

Nakahira Ken, Mukai Kazuhisa, Tamura Takashi, Inagaki Kenji, Tanaka Hidehiko

8 211 - 211 1996

Antimicrobial activity against methicillin-resistant Staphylococcus aureus in the culture broth of Enterococcus faecalis TH 10, an isolate from Malaysian fermentation food, Temph

OHHIRA Iichiro, TAMURA Takashi, FUJII Nozomi, INAGAKI Kenji, TANAKA Hidehiko

JAPANESE JOURNAL OF DAIRY AND FOOD SCIENCE 45 ( 4 ) 93 - 93-A-96 1996

Language：Japanese Publisher：Japanese Dairy Science Association

DOI： 10.11465/milkscience.45.A-93

CiNii Article

CiNii Books

Synthesis and Biochemical Studies of Selenocysteine-Containing Peptides

Other Link： https://jlc.jst.go.jp/DN/JLC/20016775976?from=CiNii

Incorporation of Fluoride Anion into Organofluoro Compounds by Microorganisms

ESAKI Nobuyoshi, TAMURA Takashi, WADA Masaru, SODA Kenji

6 ( 3 ) 117 - 118 1995.12

Tamura Takashi

Scientific reports of the Faculty of Agriculture, Okayama University 84 69 - 72 1995.2

Synthesis of fluoroacetate from fluoride, glycerol, and β- hydroxypyruvate by Streptomyces cattleya

T. Tamura, M. Wada, N. Esaki, K. Soda

Journal of Bacteriology 177 ( 9 ) 2265 - 2269 1995

Language：English

Streptomyces cattleya produces fluoroacetate and 4-fluorothreonine from inorganic fluoride added to the culture broth. We have shown by 19F nuclear magnetic resonance (NMR) spectrometry that fluoroacetate is accumulated first in the culture broth and that accumulation of 4-fluorothreonine is next. To show precursors of the carbon skeleton of fluoroacetate, we carried out tracer experiments with various 14C- and 13C-labeled compounds. Radioactivity of [U-14C]glucose, [U-14C]glycerol, [U-14C]serine, and [U-14C]β-hydroxypyruvate was incorporated into fluoroacetate to an extent of 0.2 to 0.4%, whereas [3-14C]pyruvate, [2,3-14C]succinate, and [U- 14C]aspartate were less efficiently incorporated (0.04 to 0.08%). The addition of [2-13C]glycerol to the mycelium suspension of Streptomyces cattleya caused exclusive enrichment of the carboxyl carbon of fluoroacetate with 13C
about 40% of carboxyl carbon of fluoroacetate was labeled with 13C. We studied the radioactivity incorporation of [3-14C]-, [U-14C]-, and [1-14C]β-hydroxypyruvates to show that C-2 and C-3 of β-hydroxy pyruvate are exclusively converted to the carbon skeleton of fluoroacetate. These results suggest that the carbon skeleton of fluoroacetate derives from C-1 and C-2 of glycerol through β-hydroxypyruvate, whose hydroxyl group is eventually replaced by fluoride.

Synthesis of fluoroacetate from fluoride, glycerol, and β- hydroxypyruvate by Streptomyces cattleya

T. Tamura, M. Wada, N. Esaki, K. Soda

Journal of Bacteriology 177 ( 9 ) 2265 - 2269 1995

Language：English

The mutual sparing effects of selenium and vitamin E in animal nutrition may be further explained by the discovery that mammalian thioredoxin reductase is a selenoenzyme

T Tamura, Gladyshev, V, SY Liu, TC Stadtman

BIOFACTORS 5 ( 2 ) 99 - 102 1995

Language：English Publishing type：Book review, literature introduction, etc. Publisher：IOS PRESS

The recent discovery that mammalian thioredoxin reductase is a selenoprotein furnishes an additional explanation of the mutual sparing roles of selenium and vitamin E in cellular antioxidant systems. Thioredoxin reductases isolated from human lung adenocarcinoma cells, human Jurkat T-cells and HeLa cells contain selenocysteine which is located in a C- terminal tripeptide, -Cys-SeCys-Gly.

Web of Science

Synthesis and characterization of the selenium analog of glutathione disulfide

Takashi Tamura, Tadao Oikawa, Akira Ohtaka, Nobutaka Fujii, Nobuyoshi Esaki, Kenji Soda

Analytical Biochemistry 208 ( 1 ) 151 - 154 1993

Language：English

We synthesized the selenium analog of glutathione disulfide by a liquid phase method and named it glutaselenone (i.e., γ-L-glutamyl-L-selenocysteinylglycine) diselenide. The selenol of selenocysteine was protected by the p-methoxybenzyl group, which was removed by acidolysis with trifluoroacetic acid in the presence of thioanisol. The overall yield of the final product, glutaselenone diselenide, was about 9% based on the starting compound, Se-(p-methoxybenzyl)-L-selenocysteine. Glutaselenone diselenide showed a broad absorption band between 270 and 400 nm and circular dichroism bands around 270 nm (positive) and 330 nm (negative), which were attributable to diselenide bond. © 1993 Academic Press, Inc.

DOI： 10.1006/abio.1993.1021