2021/04/08 更新

写真a

コサカ ミツコ
小阪 美津子
KOSAKA Mitsuko
所属
医歯薬学域 助教
職名
助教
外部リンク

学位

  • 医学 ( 大阪大学 )

研究キーワード

  • 発生

  • がん

  • 再生

  • 幹細胞

研究分野

  • ライフサイエンス / 腫瘍生物学

  • ライフサイエンス / 分子生物学

  • ライフサイエンス / 解剖学

学歴

  • 大阪大学大学院医学研究科    

    1988年4月 - 1992年3月

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  • 大阪大学大学院医科学修士過程    

    1986年4月 - 1988年3月

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  • 大阪大学   薬学部   薬学科

    1982年4月 - 1986年3月

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経歴

  • 岡山大学   医歯薬学総合研究科   助教

    2010年4月 - 現在

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  • 岡山県産業活性化事業

    2009年9月 - 2012年3月

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  • 理化学研究所発生再生科学総合研究センター   体性組織幹細胞研究ユニット   ユニットリーダー

    2003年10月 - 2008年3月

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  • 科学技術振興事業団   タイムシグナルと制御 領域   さきがけ21研究者

    2000年10月 - 2003年9月

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  • 京都大学   再生医科学研究所   客員助教授

    2000年4月 - 2005年3月

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  • 倉敷成人病センター   主任研究員

    1999年4月 - 2000年3月

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  • 科学技術振興財団   遺伝と変化 領域   さきがけ21研究者

    1996年10月 - 1999年9月

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  • 基礎生物学研究所   形態形成部門   助手

    1994年4月 - 1997年3月

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▼全件表示

所属学協会

 

論文

  • Prognostic value of OCT4A and SPP1C transcript variant co-expression in early-stage lung adenocarcinoma. 査読 国際誌

    Seijiro Koshimune, Mitsuko Kosaka, Nobuhiko Mizuno, Hiromasa Yamamoto, Tomoyuki Miyamoto, Kohta Ebisui, Shinichi Toyooka, Aiji Ohtsuka

    BMC cancer20 ( 1 ) 521 - 521   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Octamer-binding transcription factor 4A (OCT4A) is essential for cell pluripotency and reprogramming both in humans and mice. To date, however, the function of human OCT4 in somatic and/or tumour tissues is largely unknown. METHODS: RT-PCR was used to identify full-length splice forms of OCT4 transcripts in normal and cancer cells. A FLAG-tagged OCT4 genomic transgene was used to identify OCT4-positive cancer cells. A potential role for OCT4 in somatic cancer cells was examined by cell ablation of OCT4-positive cells using promoter-driven diphtheria toxin A. OCT4 and secreted phosphoprotein 1 (SPP1) transcripts in early-stage lung adenocarcinoma tumours were analysed and compared with pathohistological features. RESULTS: The results show that, unlike in murine cells, OCT4A and OCT4B variants are transcribed in both human cancer cells and in adult tissues such as lung, kidney, uterus, breast, and eye. We found that OCT4A and SPP1C are co-expressed in highly aggressive human breast, endometrial, and lung adenocarcinoma cell lines, but not in mesothelial tumour cell lines. Ablation of OCT4-positive cells in lung adenocarcinoma cells significantly decreased cell migration and SPP1C mRNA levels. The OCT4A/SPP1C axis was found in primary, early-stage, lung adenocarcinoma tumours. CONCLUSIONS: Co-expression of OCT4 and SPP1 may correlate with cancer aggressiveness, and the OCT4A/SPP1C axis may help identify early-stage high-risk patients with lung adenocarcinoma. Contrary to the case in mice, our data strongly suggest a critical role for OCT4A and SPP1C in the development and progression of human epithelial cancers.

    DOI: 10.1186/s12885-020-06969-0

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  • Conclusive Evidence for OCT4 Transcription in Human Cancer Cell Lines: Possible Role of a Small OCT4-Positive Cancer Cell Population 査読

    STEM CELLS36 ( 9 ) 1341 - 1354   2018年5月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/stem.2851

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  • Immediate differentiation of neuronal cells from stem/progenitor-like cells in the avian iris tissues 査読

    Tamami Matsushita, Ai Fujihara, Lars Royall, Satoshi Kagiwada, Mitsuko Kosaka, Masasuke Araki

    EXPERIMENTAL EYE RESEARCH123   16 - 26   2014年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD  

    A simple culture method that was recently developed in our laboratory was applied to the chick iris tissues to characterize neural stem/progenitor-like cells. Iris tissue is a non-neuronal tissue and does not contain any neuronal cells. In the present study we isolated iris tissues from chick embryos just prior to hatching. The isolated iris pigmented epithelium (IPE) or the stroma was embedded in Matrigel and cultured in Dulbecco's MEM supplemented with either fetal bovine serum or the synthetic serum replacement solution B27. Within 24 h of culture, elongated cells with long processes extended out from the explants of both tissues and were positively stained for various neuronal markers such as transitin, Tuj-1 and acetylated tubulin. After a longer culture period, cells positive for photoreceptor markers like rhodopsin, iodopsin and visinin were found, suggesting that the iris tissues contain retinal stem/progenitor-like cells. Several growth factors were examined to determine their effects on neuronal differentiation. EGF was shown to dramatically enhance neuronal cell differentiation, particularly the elongation of neuronal fibers. The addition of exogenous FGF2, however, did not show any positive effects on neuronal differentiation, although FGF signaling inhibitor, SU5402, suppressed neuronal differentiation. The results show that neuronal stem/progenitor-like cells can differentiate into neuronal cells immediately after they are transferred into an appropriate environment. This process did not require any exogenous factors, suggesting that neural stem/progenitor-like cells are simply suppressed from neuronal differentiation within the tissue, and isolation from the tissue releases the cells from the suppression mechanism. (C) 2014 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.exer.2014.04.007

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  • Architecture of the subendothelial elastic fibers of small blood vessels and variations in vascular type and size 査読

    Akira Shinaoka, Ryusuke Momota, Eri Shiratsuchi, Mitsuko Kosaka, Kanae Kumagishi, Ryuichi Nakahara, Ichiro Naito, Aiji Ohtsuka

    Microscopy and Microanalysis19 ( 2 ) 406 - 414   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Most blood vessels contain elastin that provides the vessels with the resilience and flexibility necessary to control hemodynamics. Pathophysiological hemodynamic changes affect the remodeling of elastic components, but little is known about their structural properties. The present study was designed to elucidate, in detail, the three-dimensional (3D) architecture of delicate elastic fibers in small vessels, and to reveal their architectural pattern in a rat model. The fine vascular elastic components were observed by a newly developed scanning electron microscopy technique using a formic acid digestion with vascular casts. This method successfully visualized the 3D architecture of elastic fibers in small blood vessels, even arterioles and venules. The subendothelial elastic fibers in such small vessels assemble into a sheet of meshwork running longitudinally, while larger vessels have a higher density of mesh and thicker mesh fibers. The quantitative analysis revealed that arterioles had a wider range of mesh density than venules
    the ratio of density to vessel size was higher than that in venules. The new method was useful for evaluating the subendothelial elastic fibers of small vessels and for demonstrating differences in the architecture of different types of vessels. © Microscopy Society of America 2013.

    DOI: 10.1017/S1431927612014341

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  • Tsukushi functions as a Wnt signaling inhibitor by competing with Wnt2b for binding to transmembrane protein Frizzled4 査読

    Kunimasa Ohta, Ayako Ito, Sei Kuriyama, Giuseppe Lupo, Mitsuko Kosaka, Shin-ichi Ohnuma, Shinichi Nakagawa, Hideaki Tanaka

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA108 ( 36 ) 14962 - 14967   2011年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    The Wnt signaling pathway is essential for the development of diverse tissues during embryogenesis. Signal transduction is activated by the binding of Wnt proteins to the type I receptor low-density lipoprotein receptor-related protein 5/6 and the seven-pass transmembrane protein Frizzled (Fzd), which contains a Wnt-binding site in the form of a cysteine-rich domain. Known extracellular antagonists of the Wnt signaling pathway can be subdivided into two broad classes depending on whether they bind primarily to Wnt or to low-density lipoprotein receptor-related protein 5/6. We show that the secreted protein Tsukushi (TSK) functions as a Wnt signaling inhibitor by binding directly to the cysteine-rich domain of Fzd4 with an affinity of 2.3 x 10(-10) M and competing with Wnt2b. In the developing chick eye, TSK is expressed in the ciliary/iris epithelium, whereas Wnt2b is expressed in the adjacent anterior rim of the optic vesicle, where it controls the differentiation of peripheral eye structures, such as the ciliary body and iris. TSK overexpression effectively antagonizes Wnt2b signaling in chicken embryonic retinal cells both in vivo and in vitro and represses Wnt-dependent specification of peripheral eye fates. Conversely, targeted inactivation of the TSK gene in mice causes expansion of the ciliary body and up-regulation of Wnt2b and Fzd4 expression in the developing peripheral eye. Thus, we uncover a crucial role for TSK as a Wnt signaling inhibitor that regulates peripheral eye formation.

    DOI: 10.1073/pnas.1100513108

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  • Tsukushi is a Frizzled ligand that, in competition with Wnt2b, regulates the proliferation of retinal stem/progenitor cells 査読

    Ohta Kunimasa, Ito Ayako, Kuriyama Sei, Lupo Giuseppe, Nakayama Rika, Ohshima Naoko, Kosaka Mitsuko, Ohnuma Shin-ichi, Nakagawa Shinichi, Tanaka Hideaki

    Mechanisms of Development126   S273   2009年

  • Molecular function of Tsukushi on neuronal stem cells 査読

    Ohta Kunimasa, Ito Ayako, Kuriyama Sei, Lupo Giuseppe, Nakayama Rika, Ohshima Naoko, Kosaka Mitsuko, Ohnuma Shin-ichi, Nakagawa Shinichi, Tanaka Hideaki

    Neuroscience Research65   S91   2009年

  • Novel Variants of Oct-3/4 Gene Expressed in Mouse Somatic Cells 査読

    Nobuhiko Mizuno, Mitsuko Kosaka

    JOURNAL OF BIOLOGICAL CHEMISTRY283 ( 45 ) 30997 - 31004   2008年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    It has been suggested that Oct-3/4 may regulate self-renewal in somatic stem cells, as it does in embryonic stem cells. However, recent reports raise the possibility that detection of human Oct-3/4 expression by RT-PCR is prone to artifacts generated by pseudogene transcripts and argue against a role for Oct-3/4 in somatic cells. In this study, we clarified Oct-3/4 expression in mouse somatic tissues using designed PCR primers, which can exclude amplification of its pseudogenes. We found that novel alternative transcripts are indeed expressed in somatic tissues, rather than the normal length transcripts in germline and ES cells. The alternative transcripts indicate the expression of two kinds of truncated proteins. Furthermore, we determined novel promoter regions that are sufficient for the expression of Oct-3/4 transcript variants in somatic cells. These findings provide new insights into the postnatal role of Oct-3/4 in somatic tissues.

    DOI: 10.1074/jbc.M802992200

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  • Tsukushi is a Frizzled4 ligand that regulates the proliferation of retinal stem cells in competition with Wnt2b 査読

    Ohta Kunimasa, Ito Ayako, Kuriyama Sei, Nakayama Rika, Oshima Naoko, Kosaka Mitsuko, Ohnuma Shinichi, Nakagawa Shinichi, Tanaka Hideaki

    Neuroscience Research61   S42   2008年

  • Multipotent cells from mammalian iris pigment epithelium 査読

    Maki Asami, Guangwei Sun, Masahiro Yamaguchi, Mitsuko Kosaka

    DEVELOPMENTAL BIOLOGY304 ( 1 ) 433 - 446   2007年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The regeneration of lens tissue from the iris of newts has become a classical model of developmental plasticity, although little is known about the corresponding plasticity of the mammalian iris. We here demonstrate and characterize multipotent cells within the iris pigment epithelium (IPE) of postnatal and adult rodents. Acutely-isolated IPE cells were morphologically homogeneous and highly pigmented, but some produced neurospheres which expressed markers characteristic of neural stem/progenitor cells. Stem/progenitor cell markers were also expressed in the IPE in vivo both neonatally and into adulthood. Inner and outer WE layers differentially expressed Nestin (Nes) in a manner suggesting that they respectively shared origins with neural retina (NR) and pigmented epithelial (RPE) layers. Transgenic marking enabled the enrichment of Nes-expressing IPE cells ex vivo, revealing a pronounced capacity to form neurospheres and differentiate into photoreceptor cells. IPE cells that did not express Nes were less able to form neurospheres, but a subset initiated the expression of pan-neural markers in primary adherent culture. These data collectively suggest that discrete populations of highly-pigmented cells with heterogeneous developmental potencies exist postnatally within the IPE, and that some of them are able to differentiate into multiple neuronal cell types. (c) 2006 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.ydbio.2006.12.047

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  • Tsukushi inhibits the proliferation of retinal stem/progenitor cells 査読

    Ohta Kunimasa, Ito Ayako, Kuriyama Sei, Ohnuma Shinichi, Kosaka Mitsuko, Nakagawa Shinichi, Tanaka H

    Developmental Biology306 ( 1 ) 391   2007年

  • Tsukushi inhibits proliferation of retinal stem cells by Wnt signalling inhibition 査読

    Ohta Kunimasa, Ito Ayako, Kuriyama Sei, Ohnuma Shin-Ichi, Kosaka Mitsuko, Nakagawa Shin-Ichi, Tanaka Hideaki

    Neuroscience Research58   S56   2007年

  • Developmental ability of cloned embryos from neural stem cells 査読

    Eiji Mizutani, Hiroshi Ohta, Satoshi Kishigami, Nguyen Van Thuan, Takafusa Hikichi, Sayaka Wakayama, Mitsuko Kosaka, Eimei Sato, Teruhiko Wakayama

    REPRODUCTION132 ( 6 ) 849 - 857   2006年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIO SCIENTIFICA LTD  

    The success rate is generally higher when cloning mice from embryonic stem (ES) cell nuclei than from somatic cell nuclei, suggesting that the embryonic nature or the undifferentiated state of the donor cell increases cloning efficiency. We assessed the developmental ability of cloned embryos derived from cultured neural stem cell (NSC) nuclei and compared the success rate with that of embryos cloned from other donor cells such as differentiated NSCs, cumulus cells, Sertoli cells and ES cells in the mouse. The transfer of two-cell cloned embryos derived from cultured NSC nuclei into surrogate mothers produced five live cloned mice. However, the success rate (0.5%) was higher in embryos cloned from cultured NSC nuclei than from differentiated NSCs (0%), but lower than that obtained by cloning mice from other cell nuclei (2.2-3.50%). Although the in vitro developmental potential to the two-cell stage of the cloned embryos derived from NSC nuclei (73%) was similar to that of the cloned embryos derived from other somatic cell nuclei (e.g., 85% in Sertoli cells and 75% in cumulus cells), the developmental rate to the morula-blastocyst stage was only 7%. This rate is remarkably lower than that produced from other somatic cells (e.g., 50% in Sertoli cells and 54% in cumulus cells). These results indicate that the undifferentiated state of neural cells does not enhance the cloning efficiency in mice and that the arrest point for in vitro development of cloned embryos depends on the donor cell type.

    DOI: 10.1530/rep.1.01010

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  • Retinal stem/progenitor properties of iris pigment epithelial cells 査読

    GW Sun, M Asami, H Ohta, J Kosaka, M Kosaka

    DEVELOPMENTAL BIOLOGY289 ( 1 ) 243 - 252   2006年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Neural stem cells/progenitors that give rise to neurons and glia have been identified in different regions of the brain.. including the embryonic retina and ciliary epithelium of the adult eye. Here, we first demonstrate the characterization of neural stem/progenitors in postnatal iris pigment epithelial (IPE) cells. Pure isolated IPE cells could form spheres that contained cells expressing retinal progenitor markers in non-adherent culture. The spheres grew by cell proliferation, as indicated by bromodeoxyuridine incorporation. When attached to laminin, the spheres forming IPE derived cells were able to exhibit neural phenotypes, including retinal-specific neurons. When co-cultured with embryonic retinal cells, or grafted into embryonic retina in vivo, the IPE cells could also display the phenotypes of photoreceptor neurons and Muller glia. Our results suggest that the IPE derived cells have retinal stem/progenitor properties and neurogenic potential without gene transfer, thereby providing a novel potential source for both basic stem cell biology and therapeutic applications for retinal diseases. (c) 2005 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.ydbio.2005.10.035

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  • Adult human retinal pigment epithelial cells capable of differentiating into neurons 査読

    K Amemiya, M Haruta, M Takahashi, M Kosaka, G Eguchi

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS316 ( 1 ) 1 - 5   2004年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    We investigated the ability of adult human RPE cells to differentiate into neurons. Two cell lines were evaluated. The cells were cultured in medium with 8% serum, transferred to a neural stem cell maintenance culture, and induced to differentiate with retinoic acid. The cells were immunocytochemically examined at each step. The cells showed epithelial-like morphology with 8% serum and all were immunoreactive for beta-III tubulin. After transfer to the stem cell maintenance culture, they changed morphologically and became immunoreactive for MAP5 and neurofilament200 after inducement with retinoic acid. The ratio of MAP5 positive cells was higher in the young adult RPE cell line. No GFAP or rod-opsin immunoreactive cells were observed. Adult human RPE cells even from old person are capable of differentiating into neurons, although the ratio of mature neurons was greater in the young than in the old cell line in this condition. (C) 2004 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2004.01.172

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  • Induction of photoreceptor-specific phenotypes in adult mammalian iris tissue 査読

    M Haruta, M Kosaka, Y Kanegae, Saito, I, T Inoue, R Kageyama, A Nishida, Y Honda, M Takahashi

    NATURE NEUROSCIENCE4 ( 12 ) 1163 - 1164   2001年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE AMERICA INC  

    DOI: 10.1038/nn762

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  • Localization of FGFR-1 in axotomized and peripheral nerve transplanted ferret retina 査読

    MZ Quan, M Kosaka, M Watanabe, Y Fukuda

    NEUROREPORT10 ( 18 ) 3903 - 3907   1999年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    RETINAL ganglion cells (RGCs) survive axotomy and regenerate their axons into the peripheral nerve graft in adult mammals. To understand the potential role of fibroblast growth factors (FGFs) in survival and regeneration of axotomized RGCs, we examined FGF receptor 1 (FGFR-1) localization in normal and PN grafted ferret retinas by immunohistochemistry in combination with retrograde labeling. Prominent expression of FGFR-1 was observed in outer plexiform layer and ganglion cell layer of normal ferret retina. In the ganglion cell layer, FGFR-1 immunoreactivity was detected in about 30% of RGCs, predominantly in large cells. In the PN grafted ferret retina, 90% of RGCs with regenerated axons expressed FGFR-1. Our findings suggest that FGFs may play an important role in the survival and axonal regeneration of RGCs of adult mammals. (C) 1999 Lippincott Williams & Wilkins.

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  • In vitro culture system for iris-pigmented epithelial cells for molecular analysis of transdifferentiation 査読

    M Kosaka, R Kodama, G Eguchi

    EXPERIMENTAL CELL RESEARCH245 ( 2 ) 245 - 251   1998年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC  

    Dissociated cells of the iris-pigmented epithelium (IPE) from a 1-day-old chick grew in monolayer culture and stably maintained their differentiated state when cultured with standard culture medium. After replacement of the control medium by EdFPH medium, which is effective in inducing dedifferentiation of retinal pigmented epithelium (RPE) cells, all cells rapidly lost pigment granules, proliferated intensively, and dedifferentiated. By further addition of ascorbic acid, dedifferentiated cells accumulated and formed a large number of lentoids, This system provides a useful opportunity for analyzing cellular and molecular mechanism involved in each step of transdifferentiation. Furthermore, Northern blot data indicates that the up-regulation of pax-6 gene could be an important event during lens regeneration as well as during normal lens development, (C) 1998 Academic Press.

    DOI: 10.1006/excr.1998.4211

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  • THE KINETICS OF INDUCTION OF HOX1.6 AND C-JUN MESSENGER-RNA DURING 3 DIFFERENT WAYS OF INDUCING DIFFERENTIATION IN TERATOCARCINOMA F9 CELLS 査読

    SA IWAI, Y NISHINA, M KOSAKA, T SUMI, T DOI, M SAKUDA, Y NISHIMUNE

    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL31 ( 6 ) 462 - 466   1995年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC IN VITRO BIOLOGY  

    Changes in Hox1.6 and c-jun gene expression were examined upon F9 cell differentiation that was induced by three independent methods: a drug treatment with retinoic acid (RA), that with sodium butyrate (NaB), and a genetic approach using the ts mutant. To obtain further information on the mechanism of teratocarcinoma cell differentiation we have examined the kinetics of the induction of Hox1.6 and c-jun mRNA whose gene products have been demonstrated to have specific roles in gene regulation. Expression of Hox1.6 mRNA was induced more rapidly than c-jun mRNA by all the above three inducing methods. Furthermore, protein synthesis was not required for the induction of Hox1.6 mRNA as well as of c-jun mRNA synthesis in ail three methods. The data suggested that the transcriptional increase in the Hox1.6 mRNA was a primary response and could play an important role in F9 cell differentiation.

    DOI: 10.1007/BF02634259

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  • INDUCTION OF TERATOCARCINOMA F9 CELL-DIFFERENTIATION WITH CIS-DIAMMINE DICHLOROPLATINUM(II) (CDDP) 査読

    T DOI, T SUMI, Y NISHINA, M KOSAKA, SA IWAI, M SAKUDA, Y NISHIMUNE

    CANCER LETTERS88 ( 1 ) 81 - 86   1995年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI PUBL IRELAND LTD  

    cis-Diammine dichloroplatinum(II) (CDDP) is the salt of a platinum compound which has been noted to have a wide spectrum of activity against malignant disorders. We have studied the effects of CDDP on embryonal carcinoma F9 cell differentiation. In the presence of this agent in vitro, the cells showed rapid morphological changes, a marked increase in the mRNA expression of various differentiation markers accompanied by a loss of tumorigenicity. These results indicate that the differentiation of F9 cells is induced with CDDP.

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  • F9-CELLS CAN BE DIFFERENTIATED TOWARD 2 DISTINCT, MUTUALLY EXCLUSIVE PATHWAYS BY RETINOIC ACID AND SODIUM-BUTYRATE 査読

    M KOSAKA, M TAKEDA, K MATSUMOTO, Y NISHIMUNE

    DEVELOPMENT GROWTH & DIFFERENTIATION36 ( 2 ) 223 - 230   1994年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL SCIENCE  

    Both retinoic acid (RA) and sodium butyrate (NaB) induce differentiation in embryonal carcinoma F9 cells. Phenotypic changes caused by RA are irreversible, whereas those of NaB are rapid and reversible. In this study, we investigated the effects of combinations of these two agents on F9 cell differentiation and showed that RA had no effect on the cells induced to differentiate with NaB and vice versa. Thus, F9 cells are induced to differentiate along two distinct pathways which are mutually exclusive.

    DOI: 10.1111/j.1440-169X.1994.00223.x

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  • THE INDUCTION OF JUN GENES DURING THE REVERSIBLE CHANGES INDUCED WITH SODIUM-BUTYRATE ON THE DIFFERENTIATION OF F9 CELLS 査読

    Y NISHINA, T SUMI, SA IWAI, M KOSAKA, Y NISHIMUNE

    EXPERIMENTAL CELL RESEARCH208 ( 2 ) 492 - 497   1993年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI: 10.1006/excr.1993.1271

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  • SUPPRESSION OF TUMORIGENICITY IN TEMPERATURE-SENSITIVE MUTANTS OF EMBRYONAL CARCINOMA-CELLS BY INDUCTION OF CELL-DIFFERENTIATION AT NONPERMISSIVE TEMPERATURE 査読

    T SUMI, Y NISHINA, M KOSAKA, M SAKUDA, Y NISHIMUNE

    INTERNATIONAL JOURNAL OF CANCER54 ( 2 ) 333 - 337   1993年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    The temperature-sensitive (ts) mutants of cell differentiation derived from the mouse teratocarcinoma cell line F9, when exposed to non-permissive temperature, undergo stem-cell differentiation concomitant with a transient retardation of cell-cycle progression. By incubating these mutant strains at non-permissive temperature, we were able to study the relationship between cell differentiation and tumorigenicity. Upon exposure to non-permissive temperature, the mutant cells undergo extensive differentiation and lose their ability to initiate tumors in vivo, but retain their in vitro proliferative potential. Our data suggest that the loss of tumorigenicity is not caused by altered proliferative potential, but rather by cell differentiation. We therefore suggest that the loss of proliferative potential and the onset of cell differentiation in teratocarcinoma F9 cells are 2 independent events which can be genetically dissected, and that there is (a) crucial step(s) in the differentiation pathway at which these ts mutant cells lose their tumorigenicity.

    DOI: 10.1002/ijc.2910540228

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  • CHANGES IN HOX1.6, C-JUN, AND OCT-3 GENE EXPRESSIONS ARE ASSOCIATED WITH TERATOCARCINOMA F9 CELL-DIFFERENTIATION IN 3 DIFFERENT WAYS OF INDUCTION 査読

    SA IWAI, M KOSAKA, Y NISHINA, T SUMI, M SAKUDA, Y NISHIMUNE

    EXPERIMENTAL CELL RESEARCH205 ( 1 ) 39 - 43   1993年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI: 10.1006/excr.1993.1055

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  • C-MYC AND P53 GENE-EXPRESSION IN THE DIFFERENTIATION OF TEMPERATURE-SENSITIVE MUTANTS OF TERATOCARCINOMA F9 CELLS 査読

    M KOSAKA, SA IWAI, Y NISHINA, T SUMI, Y NISHIMUNE

    ONCOGENE7 ( 12 ) 2455 - 2460   1992年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:STOCKTON PRESS  

    We have previously reported the isolation of several temperature-sensitive (ts) mutants of F9 cells. Further investigations showed that some mutants were induced to differentiate at non-permissive temperature of cell growth, accompanied by changes in the expression of various genes, whereas others were not. During the differentiation induced by shifting up to the nonpermissive temperature, a rapid and transient decrease in both c-myc and p53 mRNA levels and rapid induction of c-jun mRNA were observed. These changes were specific in differentiation-inducible mutants and were not observed in a non-inducible mutant. In both types of mutants, the level of c-myc mRNA decreased in association with growth retardation at the non-permissive temperature. The p53 mRNA, however, showed specific increase in the differentiation-inducible ts mutants. These observations suggest distinct roles for p53 and c-myc from proliferation to differentiation in teratocarcinoma stem cells.

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  • TERATOCARCINOMA F9 CELLS INDUCED TO DIFFERENTIATE WITH SODIUM-BUTYRATE PRODUCE BOTH TISSUE-TYPE AND UROKINASE-TYPE PLASMINOGEN ACTIVATORS 査読

    M TAKEDA, M KOSAKA, Y NISHINA, K SAWADA, K MATSUMOTO, Y NISHIMUNE

    JOURNAL OF CELLULAR BIOCHEMISTRY49 ( 3 ) 284 - 289   1992年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-LISS  

    Sodium butyrate (NaB) can induce teratocarcinoma cell differentiation as retinoic acid (RA). However, the function of these two agents seems to be a little different [Kosaka et al., Exp Cell Res, 192:46-51, 1991]. F9 cells treated with NaB synthesize both tissue-type (tPA) and urokinase-type (uPA) plasminogen activator, though RA induces only tPA production. Urokinase-type PA is demonstrated to exist in association with membrane and to localize its activity to the close environment of the cell surface. This may cause the specific cell morphology and characteristics of differentiated F9 cells induced with NaB.

    DOI: 10.1002/jcb.240490311

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  • EXPRESSION OF C-KIT PROTOONCOGENE IS STIMULATED BY CAMP IN DIFFERENTIATED F9 MOUSE TERATOCARCINOMA CELLS 査読

    Y NISHINA, Y KOBARAI, T SUMI, M KOSAKA, S NISHIKAWA, Y NISHIMUNE

    EXPERIMENTAL CELL RESEARCH198 ( 2 ) 352 - 356   1992年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI: 10.1016/0014-4827(92)90390-T

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  • REVERSIBLE EFFECTS OF SODIUM-BUTYRATE ON THE DIFFERENTIATION OF F9 EMBRYONAL CARCINOMA-CELLS 査読

    M KOSAKA, Y NISHINA, M TAKEDA, K MATSUMOTO, Y NISHIMUNE

    EXPERIMENTAL CELL RESEARCH192 ( 1 ) 46 - 51   1991年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI: 10.1016/0014-4827(91)90155-N

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  • ISOLATION OF MUTANTS SHOWING TEMPERATURE-SENSITIVE CELL-GROWTH FROM EMBRYONAL CARCINOMA-CELLS - CONTROL OF STEM-CELL DIFFERENTIATION BY INCUBATION TEMPERATURES 査読

    Y NISHIMUNE, Y NISHINA, T SUMI, M KOSAKA, M TAKEDA, K MATSUMOTO, A MATSUSHIRO, M SAKUDA

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS165 ( 1 ) 65 - 72   1989年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI: 10.1016/0006-291X(89)91034-6

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  • INHIBITION OF DNA-SYNTHESIS CAUSES STEM-CELL DIFFERENTIATION - INDUCTION OF TERATOCARCINOMA-F9 CELL-DIFFERENTIATION WITH NUCLEOSIDE ANALOGS OF DNA-SYNTHESIS INHIBITORS AND THEIR INDUCING ABILITIES COUNTERBALANCED SPECIFICALLY BY NORMAL NUCLEOSIDES 査読

    Y NISHIMUNE, M KOSAKA, Y NISHINA, T SUMI, M SAKUDA, M TAKEDA, K MATSUMOTO

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS163 ( 3 ) 1290 - 1297   1989年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

    DOI: 10.1016/0006-291X(89)91118-2

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書籍等出版物

  • Iris pigment epithelium as a potential source for both basic biology and therapeutic applications for retinal regeneration. Strategies for retinal tissue repair and regeneration in vertebrates: from fish to human

    ( 担当: 単著)

    Research Signpost  2007年 

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  • Multipotentiality of Iris Pigment Epithelial Cells in Vertebrate Eye, Adult Stem Cells

    Humana Press  2004年 

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MISC

  • Potential sources for retinal regeneration in mammals

    Mitsuko Kosaka, Maki Asami

    ZOOLOGICAL SCIENCE22 ( 12 ) 1376 - 1376   2005年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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  • Adult human retinal pigment epithelial cells can differentiate into neurone in vitro.

    K Amemiya, M Haruta, A Nishida, M Takahashi, Y Honda, G Eguchi, M Kosaka

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE42 ( 4 ) S779 - S779   2001年3月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ASSOC RESEARCH VISION OPHTHALMOLOGY INC  

    Web of Science

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  • Molecular analysis of lens transdifferentiation of pigmented epithelial cells

    M Kosaka, G Eguchi

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE40 ( 4 ) S926 - S926   1999年3月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ASSOC RESEARCH VISION OPHTHALMOLOGY INC  

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  • EGF and/or bFGF induces lens transdifferentiation of retinal pigmented epithelial cells

    M Kosaka, G Eguchi

    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE38 ( 4 ) 147 - 147   1997年3月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT-RAVEN PUBL  

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  • ANALYSIS OF TS MUTANTS OF CELL-DIFFERENTIATION IN EC CELLS

    M KOSAKA, Y NISHINA, Y NISHIMUNE

    DEVELOPMENT GROWTH & DIFFERENTIATION30 ( 4 ) 417 - 417   1988年8月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:BLACKWELL SCIENCE  

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