Faculty Profiles - KATSUHARA Maki

Papers - KATSUHARA Maki

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Analysis of the effect of permeant solutes on the hydraulic resistance of the plasma membrane in cells of Chara corallina Reviewed

Masashi Tazawa, Randy Wayne, Maki Katsuhara

Protoplasma 2024.10

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

Abstract

In the cells of Chara corallina, permeant monohydric alcohols including methanol, ethanol and 1-propanol increased the hydraulic resistance of the membrane (Lp_m⁻¹). We found that the relative value of the hydraulic resistance (^rLp_m⁻¹) was linearly dependent on the concentration (C_s) of the alcohol. The relationship is expressed in the equation: ^rLp_m⁻¹ = ρ_mC_s + 1, where ρ_m is the hydraulic resistance modifier coefficient of the membrane. Ye et al. (2004) showed that membrane-permeant glycol ethers also increased Lp⁻¹. We used their data to estimate Lp_m⁻¹ and ^rLp_m⁻¹. The values of ^rLp_m⁻¹ fit the above relation we found for alcohols. When we plotted the ρ_m values of all the permeant alcohols and glycol ethers against their molecular weights (MW), we obtained a linear curve with a slope of 0.014 M⁻¹/MW and with a correlation coefficient of 0.99. We analyzed the influence of the permeant solutes on the relative hydraulic resistance of the membrane (^rLp_m⁻¹) as a function of the external (π₀) and internal (π_i) osmotic pressures. The analysis showed that the hydraulic resistance modifier coefficients (ρ_m) were linearly related to the MW of the permeant solutes with a slope of 0.012 M⁻¹/MW and with a correlation coefficient of 0.84. The linear relationship between the effects of permeating solutes on the hydraulic resistance modifier coefficient (ρ_m) and the MW can be explained in terms of the effect of the effective osmotic pressure on the hydraulic conductivity of water channels. The result of the analysis suggests that the osmotic pressure and not the size of the permeant solute as proposed by (Ye et al., J Exp Bot 55:449–461, 2004) is the decisive factor in a solute’s influence on hydraulic conductivity. Thus, characean water channels (aquaporins) respond to permeant solutes with essentially the same mechanism as to impermeant solutes.

DOI： 10.1007/s00709-024-02000-6

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Other Link： https://link.springer.com/article/10.1007/s00709-024-02000-6/fulltext.html

In planta evidence that the HAK transporter OsHAK2 is involved in Na+ transport in rice. International journal

Shigeto Morita, Natsumi Tamba, Mineo Shibasaka, Shizuka Sasano, Taiju Kadoike, Yasuyo Urase, Masamitsu Maruyama, Aguri Fukuoka, Junta Yanai, Takehiro Masumura, Yasunari Ogihara, Shigeru Satoh, Kunisuke Tanaka, Maki Katsuhara, Hideki Nakayama

Bioscience, biotechnology, and biochemistry 87 ( 5 ) 482 - 490 2023.4

Language：English Publishing type：Research paper (scientific journal)

HAK family transporters primarily function as K+ transporters and play major roles in K+ uptake and translocation in plants, whereas several HAK transporters exhibit Na+ transport activity. OsHAK2, a rice HAK transporter, was shown to mediate Na+ transport in Escherichia coli in a previous study. In this study, we investigated whether OsHAK2 is involved in Na+ transport in the rice plant. Overexpression of OsHAK2 increased Na+ translocation from the roots to the shoots of transgenic rice. It also increased both root and whole-plant Na+ content, and enhanced shoot length under low Na+ and K+ conditions. Meanwhile, OsHAK2 overexpression increased salt sensitivity under a long-term salt stress condition, indicating that OsHAK2 is not involved in salt tolerance, unlike in the case of ZmHAK4 in maize. These results suggest that OsHAK2 is permeable to Na+ and contributes to shoot growth in rice plants under low Na+ and K+ conditions.

DOI： 10.1093/bbb/zbad020

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Distinct Functions of the Atypical Terminal Hydrophilic Domain of the HKT Transporter in the Liverwort Marchantia polymorpha. Reviewed International coauthorship International journal

Shahin Imran, Masumi Oyama, Rie Horie, Natsuko I Kobayashi, Alex Costa, Ryosuke Kumano, Chiho Hirata, Sen Thi Huong Tran, Maki Katsuhara, Keitaro Tanoi, Takayuki Kohchi, Kimitsune Ishizaki, Tomoaki Horie

Plant & Cell Physiology 63 ( 6 ) 802 - 816 2022.6

Language：English Publishing type：Research paper (scientific journal)

K+/Na+ homeostasis is important for land plants, particularly under salt stress. In this study, the structure and ion transport properties of the high-affinity K+ transporter (HKT) of the liverwort Marchantia polymorpha were investigated. Only one HKT gene, MpHKT1, was identified in the genome of M. polymorpha. Phylogenetic analysis of HKT proteins revealed that non-seed plants possess HKTs grouped into a clade independent of the other two clades including HKTs of angiosperms. A distinct long hydrophilic domain was found in the C-terminus of MpHKT1. Complementary DNA (cDNA) of truncated MpHKT1 (t-MpHKT1) encoding the MpHKT_Δ596-812 protein was used to examine the functions of the C-terminal domain. Both MpHKT1 transporters fused with enhanced green fluorescent protein at the N-terminus were localized to the plasma membrane when expressed in rice protoplasts. Two-electrode voltage clamp experiments using Xenopus laevis oocytes indicated that MpHKT1 mediated the transport of monovalent alkali cations with higher selectivity for Na+ and K+, but truncation of the C-terminal domain significantly reduced the transport activity with a decrease in the Na+ permeability. Overexpression of MpHKT1 or t-MpHKT1 in M. polymorpha conferred accumulation of higher Na+ levels and showed higher Na+ uptake rates, compared to those of wild-type plants; however, phenotypes with t-MpHKT1 were consistently weaker than those with MpHKT1. Together, these findings suggest that the hydrophilic C-terminal domain plays a unique role in the regulation of transport activity and ion selectivity of MpHKT1.

DOI： 10.1093/pcp/pcac044

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Calcium control of the hydraulic resistance in cells of Chara corallina Reviewed International coauthorship International journal

Masashi Tazawa, Maki Katsuhara, Randy Wayne

Protoplasma 260 ( 1 ) 299 - 306 2022.6

Identification and Characterization of Rice OsHKT1;3 Variants Reviewed International journal

Shahin Imran, Yoshiyuki Tsuchiya, Sen Thi Huong Tran, Maki Katsuhara

Plants 10 ( 10 ) 2006 - 2006 2021.9

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

In rice, the high-affinity K+ transporter, OsHKT1;3, functions as a Na+-selective transporter. mRNA variants of OsHKT1;3 have been reported previously, but their functions remain unknown. In this study, five OsHKT1;3 variants (V1-V5) were identified from japonica rice (Nipponbare) in addition to OsHKT1;3_FL. Absolute quantification qPCR analyses revealed that the transcript level of OsHKT1;3_FL was significantly higher than other variants in both the roots and shoots. Expression levels of OsHKT1;3_FL, and some variants, increased after 24 h of salt stress. Two electrode voltage clamp experiments in a heterologous expression system using Xenopus laevis oocytes revealed that oocytes expressing OsHKT1;3_FL and all of its variants exhibited smaller Na+ currents. The presented data, together with previous data, provide insights to understanding how OsHKT family members are involved in the mechanisms of ion homeostasis and salt tolerance in rice.

DOI： 10.3390/plants10102006

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Physiological Role of Aerobic Fermentation Constitutively Expressed in an Aluminum-Tolerant Cell Line of Tobacco (Nicotiana tabacum) Reviewed International journal

Tsuchiya Y, Nakamura T, Izumi Y, Okazaki K, Shinano T, Kubo Y, Katsuhara M, Sasaki T, Yamamoto Y

Plant & Cell Physiology 62 ( 9 ) 1460 - 1477 2021.9

Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

<title>Abstract</title>
Aluminum (Al)-tolerant tobacco cell line ALT301 derived from SL (wild-type) hardly exhibits Al-triggered reactive oxygen species (ROS) compared with SL. Molecular mechanism leading to this phenotype was investigated comparatively with SL. Under normal growth condition, metabolome data suggested the activation of glycolysis and lactate fermentation but the repression of the tricarboxylic acid (TCA) cycle in ALT301, namely aerobic fermentation, which seemed to be transcriptionally controlled partly by higher expression of genes encoding lactate dehydrogenase and pyruvate dehydrogenase kinase. Microarray and gene ontology analyses revealed the upregulation of th e gene encoding related to APETALA2.3 (RAP2.3)-like protein, one of the group VII ethylene response factors (ERFVIIs), in ALT301. ERFVII transcription factors are known to be key regulators for hypoxia response that promotes substrate-level ATP production by glycolysis and fermentation. ERFVIIs are degraded under normoxia by the N-end rule pathway of proteolysis depending on both oxygen and nitric oxide (NO), and NO is produced mainly by nitrate reductase (NR) in plants. In ALT301, levels of the NR gene expression (NIA2), NR activity and NO production were all lower compared with SL. Consistently, the known effects of NO on respiratory pathways were also repressed in ALT301. Under Al-treatment condition, NO level increased in both lines but was lower in ALT301. These results suggest that the upregulation of the RAP2.3-like gene and the downregulation of the NIA2 gene and resultant NO depletion in ALT301 coordinately enhance aerobic fermentation, which seems to be related to a higher capacity to prevent ROS production in mitochondria under Al stress.

DOI： 10.1093/pcp/pcab098

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Age dependence of the hydraulic resistances of the plasma membrane and the tonoplast (vacuolar membrane) in cells of Chara corallina Reviewed International coauthorship International journal

Masashi Tazawa, Maki Katsuhara, Randy Wayne

Protoplasma 258 ( 4 ) 793 - 801 2021.7

Erratum: Physiological role of aerobic fermentation constitutively expressed in an aluminum-tolerant cell line of tobacco (Nicotiana Tabacum) (Plant and Cell Physiology DOI: 10.1093/pcp/pcab098)

Yoshiyuki Tsuchiya, Takuji Nakamura, Yohei Izumi, Keiki Okazaki, Takuro Shinano, Yasutaka Kubo, Maki Katsuhara, Takayuki Sasaki, Yoko Yamamoto

Plant and Cell Physiology 62 ( 6 ) 1058 2021.6

Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press

In the originally published version of this manuscript, the ethylene signaling pathway in Arabidopsis shown in the Figure 3 was incorrectly referred. The correct pathway is as follows: Ethylene -| ETR1 -&gt
CTR1 -| EIN2 -&gt
EIN3/EIL1 -&gt
ERFs. In addition, information was missing from the Figure 3 caption. An explanation of the subgroups of the ERF family shown in the figure (Group IX, VIII, VI, VII) has been added to the caption, which also necessitated two new references in the paper. The corrected caption is: Expression of the genes encoding transcription factors (EIL, ERFs) and chitinases (CHNs) associated with ethylene signaling pathway in SL and ALT301 under normal growth condition. Gene expression analysis was performed by real-time RT-PCR. Values are the mean±SE (n=3–5 from three to five independent experiments, respectively). Significant differences between SL and ALT301 are indicated with asterisks (*P &lt
0.05, **P &lt
0.01, Welch’s t-test). Ethylene signaling pathway in Arabidopsis (Kazan 2015) is shown (top). Group names of the ERF genes are indicated, which is based on Rushton et al. (2008) and Nakano et al. (2006). Note that some of the ERF genes have several synonyms (see the National Center for Biotechnology Information [NCBI] database using the gene accessions listed in Supplementary Table S6). Abbreviations: ETR1, Ethylene Response1
CTR1, Constitutive Triple Response1
EIN2, Ethylene Insensitive2
EIN3, Ethylene Insensitive3
EIL1, Ethylene Insensitive-Like Protein1
ERF, Ethylene Response Factor. The gene Tsi1 encodes Tobacco stress-induced gene1. The two new references are: Nakano, T., Suzuki, K., Fujimura, T. and Shinshi, H. (2006) Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol. 140: 411–432. Rushton, P.J., Bokowiec, M.T., Han, S., Zhang, H., Brannock, J.F., Chen, X., et al. (2008) Tobacco transcription factors: novel insights into transcriptional regulation in the Solanaceae. Plant Physiol. 147: 280–295. These errors have been corrected.

DOI： 10.1093/pcp/pcab131

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Mechanisms Activating Latent Functions of PIP Aquaporin Water Channels via the Interaction between PIP1 and PIP2 Proteins Reviewed International journal

Mineo Shibasaka, Tomoaki Horie, Maki Katsuhara

Plant & Cell Physiology 62 ( 1 ) 92 - 99 2021.3

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

<title>Abstract</title>
Plant plasma membrane-type plasma membrane intrinsic protein (PIP) aquaporins are classified into two groups, PIP1s and PIP2s. In this study, we focused on HvPIP1;2, a PIP1 in barley (Hordeum vulgare), to dissect the molecular mechanisms that evoke HvPIP1-mediated water transport. No HvPIP1;2 protein was localized to the plasma membrane when expressed alone in Xenopus laevis oocytes. By contrast, a chimeric HvPIP1;2 protein (HvPIP1;2_24NC), in which the N- and C-terminal regions were replaced with the corresponding regions from HvPIP2;4, was found to localize to the plasma membrane of oocytes. However, HvPIP1;2_24NC showed no water transport activity in swelling assays. These results suggested that the terminal regions of PIP2 proteins direct PIP proteins to the plasma membrane, but the relocalization of PIP1 proteins was not sufficient to PIP1s functionality as a water channel in a membrane. A single amino acid replacement of threonine by methionine in HvPIP2;4 (HvPIP2;4T229M) abolished water transport activity. Co-expression of HvPIP1;2_24NC either with HvPIP2;4_12NC or with HvPIP2;4TM_12NC, in which the N- and C-terminal regions were replaced with the corresponding regions of HvPIP1;2, increased the water transport activity in oocytes. These data provided evidence that the HvPIP1;2 molecule has own water transport activity and an interaction with the middle part of the HvPIP2;4 protein (except for the N- and C-termini) is required for HvPIP1;2 functionality as a water channel. This molecular mechanism could be applied to other PIP1s and PIP2s in addition to the known mechanism that the terminal regions of some PIP2s lead some PIP1s to the plasma membrane.

DOI： 10.1093/pcp/pcaa142

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Other Link： http://academic.oup.com/pcp/article-pdf/62/1/92/36718180/pcaa142.pdf

Accession difference in leaf photosynthesis, root hydraulic conductance and gene expression of root aquaporins under salt stress in barley seedlings Reviewed International coauthorship International journal

Asuka Kodama, Tamaki Watanabe, Makoto Yamaguchi, Ryohei Narita, Maki Katsuhara, Kazuhiro Sato, Taiichiro Ookawa, Tadashi Hirasawa

Plant Production Science 24 ( 1 ) 73 - 82 2021.1

A Survey of Barley PIP Aquaporin Ionic Conductance Reveals Ca2+-Sensitive HvPIP2;8 Na+ and K+ Conductance Reviewed International coauthorship International journal

Sen Thi Huong Tran, Tomoaki Horie, Shahin Imran, Jiaen Qiu, Samantha McGaughey, Caitlin S. Byrt, Stephen D. Tyerman, Maki Katsuhara

International Journal of Molecular Sciences 21 ( 19 ) 7135 - 7135 2020.9

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Some plasma membrane intrinsic protein (PIP) aquaporins can facilitate ion transport. Here we report that one of the 12 barley PIPs (PIP1 and PIP2) tested, HvPIP2;8, facilitated cation transport when expressed in Xenopus laevis oocytes. HvPIP2;8-associated ion currents were detected with Na+ and K+, but not Cs+, Rb+, or Li+, and was inhibited by Ba2+, Ca2+, and Cd2+ and to a lesser extent Mg2+, which also interacted with Ca2+. Currents were reduced in the presence of K+, Cs+, Rb+, or Li+ relative to Na+ alone. Five HvPIP1 isoforms co-expressed with HvPIP2;8 inhibited the ion conductance relative to HvPIP2;8 alone but HvPIP1;3 and HvPIP1;4 with HvPIP2;8 maintained the ion conductance at a lower level. HvPIP2;8 water permeability was similar to that of a C-terminal phosphorylation mimic mutant HvPIP2;8 S285D, but HvPIP2;8 S285D showed a negative linear correlation between water permeability and ion conductance that was modified by a kinase inhibitor treatment. HvPIP2;8 transcript abundance increased in barley shoot tissues following salt treatments in a salt-tolerant cultivar Haruna-Nijo, but not in salt-sensitive I743. There is potential for HvPIP2;8 to be involved in barley salt-stress responses, and HvPIP2;8 could facilitate both water and Na+/K+ transport activity, depending on the phosphorylation status.

DOI： 10.3390/ijms21197135

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Na+ Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na+ Concentration and Regulates Shoot Na+ Concentration Reviewed International journal

Yuki Kawakami, Shahin Imran, Maki Katsuhara, Yuichi Tada

International Journal of Molecular Sciences 21 ( 17 ) 6100 - 6100 2020.8

Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

We characterized an Na+ transporter SvHKT1;1 from a halophytic turf grass, Sporobolus virginicus. SvHKT1;1 mediated inward and outward Na+ transport in Xenopus laevis oocytes and did not complement K+ transporter-defective mutant yeast. SvHKT1;1 did not complement athkt1;1 mutant Arabidopsis, suggesting its distinguishable function from other typical HKT1 transporters. The transcript was abundant in the shoots compared with the roots in S. virginicus and was upregulated by severe salt stress (500 mM NaCl), but not by lower stress. SvHKT1;1-expressing Arabidopsis lines showed higher shoot Na+ concentrations and lower salt tolerance than wild type (WT) plants under nonstress and salt stress conditions and showed higher Na+ uptake rate in roots at the early stage of salt treatment. These results suggested that constitutive expression of SvHKT1;1 enhanced Na+ uptake in root epidermal cells, followed by increased Na+ transport to shoots, which led to reduced salt tolerance. However, Na+ concentrations in phloem sap of the SvHKT1;1 lines were higher than those in WT plants under salt stress. Based on this result, together with the induction of the SvHKT1;1 transcription under high salinity stress, it was suggested that SvHKT1;1 plays a role in preventing excess shoot Na+ accumulation in S. virginicus.

DOI： 10.3390/ijms21176100

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Ectopic expression of a rice plasma membrane intrinsic protein (OsPIP1;3) promotes plant growth and water uptake Reviewed International coauthorship International journal

Siyu Liu, Tatsuya Fukumoto, Patrizia Gena, Peng Feng, Qi Sun, Qiang Li, Tadashi Matsumoto, Toshiyuki Kaneko, Hang Zhang, Yao Zhang, Shihua Zhong, Weizhong Zeng, Maki Katsuhara, Yoshichika Kitagawa, Aoxue Wang, Giuseppe Calamita, Xiaodong Ding

The Plant Journal 102 ( 4 ) 779 - 796 2020.5

Expression and Ion Transport Activity of Rice OsHKT1;1 Variants Reviewed International journal

Shahin Imran, Tomoaki Horie, Maki Katsuhara

Plants 9 ( 1 ) 16 - 16 2019.12

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

OsHKT1;1 in rice, belongs to the high-affinity K+ Transporter family, has been found to be involved in salt tolerance. OsHKT1;1 in japonica rice (Nipponbare) produces mRNA variants, but their functions remain elusive. In salt tolerant rice, Pokkali, eight OsHKT1;1 variants (V1-V8) were identified in addition to the full-length OsHKT1;1 (FL) cDNA. Absolute quantification by qPCR revealed that accumulation of OsHKT1;1-FL mRNA is minor in contrast to that of OsHKT1;1-V1, -V2, -V4, and -V7 mRNAs, all of which are predominant in shoots, while only V1 and V7 mRNAs are predominant in roots. Two electrode voltage clamp (TEVC) experiments using Xenopus laevis oocytes revealed that oocytes-expressing OsHKT1;1-FL from Pokkali exhibited inward-rectified currents in the presence of 96 mM Na+ as reported previously. Further TEVC analyses indicated that six of eight OsHKT1;1 variants elicited currents in a Na+ or a K+ bath solution. OsHKT1;1-V6 exhibited a similar inward rectification to the FL protein. Contrastingly, however, the rests mediated bidirectional currents in both Na+ and K+ bath solutions. These data suggest possibilities that novel mechanisms regulating the transport activity of OsHKT1;1 might exist, and that OsHKT1;1 variants might also carry out distinct physiological roles either independently or in combination with OsHKT1;1-FL.

DOI： 10.3390/plants9010016

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Dynamics of the contents and distribution of ABA, auxins and aquaporins in developing caryopses of an ABA-deficient barley mutant and its parental cultivar Reviewed

Oksana A. Seldimirova, Guzel R. Kudoyarova, Maki Katsuhara, Ilshat R. Galin, Denis Yu. Zaitsev, Natalia N. Kruglova, Dmitry S. Veselov, Stanislav Yu. Veselov

Seed Science Research 29 ( 4 ) 261 - 269 2019.12

Language：English Publishing type：Research paper (scientific journal) Publisher：Cambridge University Press (CUP)

<title>Abstract</title>Dynamics of abscisic acid (ABA) and indole-3-acetic acid (IAA) contents were followed in developing barley caryopses of the ABA-deficient mutant AZ34 and its parental cultivar Steptoe. Distribution of these hormones and HvPIP2 aquaporins (AQPs) was studied with the help of immunohistochemical methods in the roots and coleorhiza of developing embryos. In Steptoe, maturation of the caryopsis was accompanied by vast accumulation of ABA, while this hormone accumulated more slowly in the caryopsis of AZ34 and its content was lower than in Steptoe. Accumulation of ABA was accompanied by a decline in IAA level in the developing caryopsis, the process being delayed in AZ34 in accordance with the slower accumulation of ABA. ABA accumulated to high levels in the coleorhiza cells of Steptoe, while the effect was absent in AZ34. The high level of ABA was likely to be important for maintaining the barrier function of the coleorhiza, preventing germination of seminal roots and enabling seed dormancy, while the absence of ABA accumulation in coleorhiza of AZ34 may be responsible for the initiation of root germination inside the caryopsis. The abundance of HvPIP2 AQPs in the seminal roots was higher at the beginning of maturation of Steptoe caryopsis and declined afterwards, while the levels of APQs increased later in AZ34 in accordance with the delay in ABA accumulation. These results suggest the importance of ABA accumulation in coleorhiza for preventing precocious growth of seminal roots, and suggest regulation of IAA and aquaporin levels by this hormone during maturation of embryos.

DOI： 10.1017/s0960258519000229

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The Mechanism of SO₂ -Induced Stomatal Closure Differs from O₃ and CO₂ Responses and Is Mediated by Non-Apoptotic Cell Death in Guard Cells. Reviewed International journal

Ooi L, Matsuura T, Munemasa S, Murata Y, Katsuhara M, Hirayama T, Mori IC

Plant, cell & environment 42 ( 2 ) 437 - 447 2019.2

Language：English Publishing type：Research paper (scientific journal)

Plants closing stomata in the presence of harmful gases is believed to be a stress avoidance mechanism. SO2 , one of the major airborne pollutants, has long been reported to induce stomatal closure, yet the mechanism remains unknown. Little is known about the stomatal response to airborne pollutants besides O3 . SLOW ANION CHANNEL-ASSOCIATED 1 (SLAC1) and OPEN STOMATA 1 (OST1) were identified as genes mediating O3 -induced closure. SLAC1 and OST1 are also known to mediate stomatal closure in response to CO2 , together with RESPIRATORY BURST OXIDASE HOMOLOGs (RBOHs). The overlaying roles of these genes in response to O3 and CO2 suggested that plants share their molecular regulators for airborne stimuli. Here, we investigated and compared stomatal closure event induced by a wide concentration range of SO2 in Arabidopsis through molecular genetic approaches. O3 - and CO2 -insensitive stomata mutants did not show significant differences from the wild type in stomatal sensitivity, guard cell viability, and chlorophyll content revealing that SO2 -induced closure is not regulated by the same molecular mechanisms as for O3 and CO2 . Nonapoptotic cell death is shown as the reason for SO2 -induced closure, which proposed the closure as a physicochemical process resulted from SO2 distress, instead of a biological protection mechanism.

DOI： 10.1111/pce.13406

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High-affinity K+ transporters from a halophyte, Sporobolus virginicus, mediate both K+ and Na+ transport in transgenic Arabidopsis, X. laevis oocytes, and yeast. Reviewed

Tada Y, Endo C, Katsuhara M, Horie T, Shibasaka M, Nakahara Y, Kurusu T

Plant & Cell Physiology 60 ( 1 ) 176 - 187 2019.1

Functional screening of salt tolerance genes from a halophyte Sporobolus virginicus and transcriptomic and metabolomic analysis of salt tolerant plants expressing glycine-rich RNA-binding protein Reviewed

Yuichi Tada, Ryuichi Kawano, Shiho Komatsubara, Hideki Nishimura, Maki Katsuhara, Soichi Ozaki, Shin Terashima, Kentaro Yano, Chisato Endo, Muneo Sato, Mami Okamoto, Yuji Sawada, Masami Yokota Hirai, Takamitsu Kurusu

Plant Science 278 54 - 63 2019.1

Energy costs of salinity tolerance in crop plants Invited Reviewed

Stephen D. Tyerman, Rana Munns, Wieland Fricke, Borjana Arsova, Bronwyn J. Barkla, Jayakumar Bose, Helen Bramley, Caitlin Byrt, Zhonghua Chen, Timothy D. Colmer, Tracey Cuin, David A. Day, Kylie J. Foster, Matthew Gilliham, Sam W. Henderson, Tomoaki Horie, Colin L. D. Jenkins, Brent N. Kaiser, Maki Katsuhara, Darren Plett, Stanley J. Miklavcic, Stuart J. Roy, Francisco Rubio, Sergey Shabala, Megan Shelden, Kathleen Soole, Nicolas L. Taylor, Mark Tester, Michelle Watt, Stefanie Wege, Lars H. Wegner, Zhengyu Wen

New Phytologist 221 ( 1 ) 25 - 29 2019.1

A Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na+ and K+ and Permeable to Na+ and K+ Non-Selectively Reviewed International journal

Izumi Mori, Yuichi Nobukiyo, Yoshiki Nakahara, Mineo Shibasaka, Takuya Furuichi, Maki Katsuhara

Plants 7 ( 3 ) 61 - 61 2018.7

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Cyclic nucleotide-gated channels (CNGCs) have been postulated to contribute significantly in plant development and stress resistance. However, their electrophysiological properties remain poorly understood. Here, we characterized barley CNGC2-3 (HvCNGC2-3) by the two-electrode voltage-clamp technique in the Xenopus laevis oocyte heterologous expression system. Current was not observed in X. laevis oocytes injected with HvCNGC2-3 complementary RNA (cRNA) in a bathing solution containing either Na+ or K+ solely, even in the presence of 8-bromoadenosine 3′,5′-cyclic monophosphate (8Br-cAMP) or 8-bromoguanosine 3′,5′-cyclic monophosphate (8Br-cGMP). A weakly voltage-dependent slow hyperpolarization-activated ion current was observed in the co-presence of Na+ and K+ in the bathing solution and in the presence of 10 µM 8Br-cAMP, but not 8Br-cGMP. Permeability ratios of HvCNGC2-3 to K+, Na+ and Cl− were determined as 1:0.63:0.03 according to reversal-potential analyses. Amino-acid replacement of the unique ion-selective motif of HvCNGC2-3, AQGL, with the canonical motif, GQGL, resulted in the abolition of the current. This study reports a unique two-ion-dependent activation characteristic of the barley CNGC, HvCNGC2-3.

DOI： 10.3390/plants7030061

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Papers - KATSUHARA Maki