研究者詳細 - 世良　貴史

論文 - 世良　貴史

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Necessity of Flanking Repeats R1′ and R8′ of Human Pumilio1 Protein for RNA Binding 査読

Kento Nakamura, Taishu Nakao, Tomoaki Mori, Serika Ohno, Yusuke Fujita, Keisuke Masaoka, Kazuki Sakabayashi, Koichi Mori, Takamasa Tobimatsu, Takashi Sera

Biochemistry 60 ( 40 ) 3007 - 3015 2021年9月

Genome sequence analysis of new plum pox virus isolates from Japan 査読国際誌

Tomoaki Mori, Chiaki Warner, Serika Ohno, Koichi Mori, Takamasa Tobimatsu, Takashi Sera

BMC Research Notes 14 ( 1 ) 266 - 266 2021年7月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Springer Science and Business Media LLC

<title>Abstract</title><sec>
<title>Objective</title>
To find mutations that may have recently occurred in <italic>Plum pox virus</italic> (PPV), we collected six PPV-infected plum/peach trees from the western part of Japan and one from the eastern part. After sequencing the full-length PPV genomic RNAs, we compared the amino acid sequences with representative isolates of each PPV strain.

</sec><sec>
<title>Results</title>
All new isolates were found to belong to the PPV-D strain: the six isolates collected from western Japan were identified as the West-Japan strain while the one collected from eastern Japan as the East-Japan strain. Amino acid sequence analysis of these seven isolates suggested that the 1407th and 1529th amino acid residues are characteristic of the West-Japan and the East-Japan strains, respectively. Comparing them with the corresponding amino acid residues of the 47 non-Japanese PPV-D isolates revealed that these amino acid residues are undoubtedly unique. A further examination of the relevant amino acid residues of the other 210 PPV-D isolates collected in Japan generated a new hypothesis regarding the invasion route from overseas and the subsequent diffusion route within Japan: a PPV-D strain might have invaded the western part of Japan from overseas and spread throughout Japan.

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DOI： 10.1186/s13104-021-05683-9

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その他リンク： https://link.springer.com/article/10.1186/s13104-021-05683-9/fulltext.html

Site-specific integration by recruitment of a complex of ΦC31integrase and donor DNA to a target site by using a tandem, artificial zinc-finger protein 査読国際誌

Biochemistry 57 ( 50 ) 6868 - 6877 2018年11月

Targeted silencing of SOX2 by an artificial transcription factor showed antitumor effect in lung and esophageal squamous cell carcinoma 査読国際誌

Etsuko Yokota,Tomoki Yamatsuji,Munenori Takaoka,Minoru Haisa ,Nagio Takigawa,Noriko Miyake,Tomoko Ikeda,Tomoaki Mori*,Serika Ohno,Takashi Sera*,Takuya Fukazawa,Yoshio Naomoto

Oncotarget 8 ( 61 ) 103063 - 103076 2017年1月

Cleavage of influenza RNA by using a human PUF-based artificial RNA-binding protein?staphylococcal nuclease hybrid 査読国際誌

Biochem. Biophys. Res. Commun. 479 ( 4 ) 736 - 740 2016年9月

Gene- and Protein-Delivered Zinc Finger–Staphylococcal Nuclease Hybrid for Inhibition of DNA Replication of Human Papillomavirus 査読

Takashi Mino, Tomoaki Mori, Yasuhiro Aoyama, Takashi Sera

PLoS ONE 8 ( 2 ) e56633 - e56633 2013年2月

Enhanced Cleavage of Double-Stranded DNA by Artificial Zinc-Finger Nuclease Sandwiched between Two Zinc-Finger Proteins 査読

Yusuke Mineta, Tomoyuki Okamoto, Kosuke Takenaka, Norio Doi, Yasuhiro Aoyama, Takashi Sera

Biochemistry 47 12257 - 12259 2008年11月

Modulation of endogenous VEGF-A expression by artificial transcription factors.

Tomoaki Mori, Jun Sasaki, Yasuhiro Aoyama, Takashi Sera

Nucleic acids symposium series (2004) ( 51 ) 345 - 346 2007年

記述言語：英語掲載種別：研究論文（学術雑誌）

The vascular endothelial growth factor A (VEGF-A) gene is an attractive therapeutic target because both activation and repression of the gene are useful for treatment or cure of many diseases related to abnormal angiogenesis. To modulate the endogenous gene expression artificially, we previously designed a six-finger AZP to recognize a 19-bp DNA in the VEGF-A gene, and fused the AZP with a nuclear localization signal and a repressor domain to generate an artificial transcription factor (ATF). Using the ATF, we demonstrated efficient modulation of the VEGF-A expression. In the present study, we evaluate the ability of the ATF to modulate the gene expression in more detail. First we examined the ability of the ATF under hypoxia condition. ELISA of the VEGF-A protein in the culture medium revealed that the gene-delivered ATF repressed the expression rate of the VEGF-A gene under hypoxia condition.

DOI： 10.1093/nass/nrm173

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Development of protein-based antiviral drugs for human papillomaviruses.

Takashi Mino, Tomoaki Mori, Yasuhiro Aoyama, Takashi Sera

Nucleic acids symposium series (2004) ( 51 ) 427 - 428 2007年

記述言語：英語掲載種別：研究論文（学術雑誌）

Recently, we designed artificial zinc-finger proteins (AZPs) that prevent a viral replication protein, E2, of human papillomavirus type 18 (HPV-18) from binding to its replication origin and demonstrated that the gene-delivered AZPs inhibited HPV-18 DNA replication in mammalian cells. In the present study, we examined another approach to delivery of AZPs. We constructed cell-permeable AZPs by fusing an AZP previously generated for inhibition of HPV-18 DNA replication with a cell-penetrating peptide (CPP), and confirmed that these CPP-AZP fusions reduced the replication rate in transient replication assays when added to the culture medium. In particular, 250 nM CPP-AZP reduced HPV-18 DNA replication to 3% of that of a control experiment. Western blot analysis detected 7% of the CPP-AZP added to the culture medium in the cell lysates, and demonstrated that greater internalization of CPP-AZP into mammalian cells causes greater inhibition of viral DNA replication. Furthermore, CPP-AZP did not show any significant cytotoxixity in MTT assays. Thus, our results demonstrate that cell-permeable AZPs could serve as potent protein-based antiviral drugs.

DOI： 10.1093/nass/nrm214

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Inhibition of DNA replication of human papillomavirus by artificial zinc finger proteins. 査読国際誌

Takashi Mino, Takeaki Hatono, Naoki Matsumoto, Tomoaki Mori, Yusuke Mineta, Yasuhiro Aoyama, Takashi Sera

Journal of virology 80 ( 11 ) 5405 - 5412 2006年6月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Recently, we demonstrated that plant DNA virus replication was inhibited in planta by using an artificial zinc finger protein (AZP) and created AZP-based transgenic plants resistant to DNA virus infection. Here we apply the AZP technology to the inhibition of replication of a mammalian DNA virus, human papillomavirus type 18 (HPV-18). Two AZPs, designated AZP(HPV)-1 and AZP(HPV)-2, were designed by using our nondegenerate recognition code table and were constructed to block binding of the HPV-18 E2 replication protein to the replication origin. Both of the newly designed AZPs had much higher affinities towards the replication origin than did the E2 protein, and they efficiently blocked E2 binding in vitro. In transient replication assays, both AZPs inhibited viral DNA replication, especially AZP(HPV)-2, which reduced the replication level to approximately 10%. We also demonstrated in transient replication assays, using plasmids with mutant replication origins, that AZP(HPV)-2 could precisely recognize the replication origin in mammalian cells. Thus, it was demonstrated that the AZP technology could be applied not only to plant DNA viruses but also to mammalian DNA viruses.

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Inhibition of Virus DNA Replication by Artificial Zinc Finger Proteins 査読

Takashi Sera

Journal of Virology 79 ( 4 ) 2614 - 2619 2005年2月

担当区分：筆頭著者,　最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：American Society for Microbiology

ABSTRACT

Prevention of virus infections is a major objective in agriculture and human health. One attractive approach to the prevention is inhibition of virus replication. To demonstrate this concept in vivo, an artificial zinc finger protein (AZP) targeting the replication origin of theBeet severe curly top virus(BSCTV), a model DNA virus, was created. In vitro DNA binding assays indicated that the AZP efficiently blocked binding of the viral replication protein (Rep), which initiates virus replication, to the replication origin. All of the transgenicArabidopsisplants expressing the AZP showed phenotypes strongly resistant to virus infection, and 84% of the transgenic plants showed no symptom. Southern blot analysis demonstrated that BSCTV replication was completely suppressed in the transgenic plants. Since the mechanism of viral DNA replication is well conserved among plants and mammals, this approach could be applied not only to agricultural crop protection but also to the prevention of virus infections in humans.

DOI： 10.1128/jvi.79.4.2614-2619.2005

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Regulation of the endogenous VEGF-A gene by exogenous designed regulatory proteins 査読

Kiyoshi Tachikawa, Oliver Schröder, Gerhard Frey, Steven P. Briggs, Takashi Sera

Proceedings of the National Academy of Sciences 101 ( 42 ) 15225 - 15230 2004年10月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Proceedings of the National Academy of Sciences

We describe a facile method to activate or repress transcription of endogenous genes in a quantitative and specific manner by treatment with designed regulatory proteins (DRPs), in which artificial transcription factors (ATFs) are fused to cell-penetrating peptides (CPPs). Penetration of DRPs into cells is mediated by an N-terminal CPP fused to a nuclear localization signal; a DNA-binding domain and a transactivation domain follow. The DNA-binding domain was targeted to the vascular endothelial growth factor (VEGF)-A gene. An agonist DRP was rapidly taken up by cells and transported to the nucleus; soon after, the cells began transcribing the gene and secreting VEGF-A protein in a dose-dependent manner. Multiple copies of a short oligopeptide derived from a minimal transactivation domain of human β-catenin was stronger than VP-16. The SRDX domain from the plant transcription factor, SUPERMAN, changed the DRP to a hypoxia-induced antagonist of VEGF-A. DRPs combine many of the potential benefits of transgenes with those of recombinant proteins.

DOI： 10.1073/pnas.0406473101

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