2024/02/02 更新

写真a

ヤマジ ナオキ
山地 直樹
YAMAJI Naoki
所属
資源植物科学研究所 准教授
職名
准教授

学位

  • 博士(農学) ( 2005年3月   愛媛大学 )

研究キーワード

  • 酸性土壌

  • 有害金属

  • 耐性遺伝子

  • 選択性

  • ペプチド

  • ケイ酸トランスポーター

  • 適応機構

  • 極性

  • ケイ酸

  • 吸収

  • 品種間差

  • 養分欠乏

  • 輸送基質

  • 金属毒性

  • カドミウム

  • トランスポーター

  • ヒ素

  • 輸送体

  • 植物成長・生理

  • 植物栄養

  • 植物生長・生理

  • 分配

  • ミネラル

  • 転写因子

  • イネ

  • アルミニウム

  • 耐性

研究分野

  • ライフサイエンス / 植物栄養学、土壌学

  • ライフサイエンス / 植物分子、生理科学

経歴

  • 岡山大学   准教授

    2015年

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論文

  • Local distribution of manganese to leaf sheath is mediated by OsNramp5 in rice. 国際誌

    Sheng Huang, Noriyuki Konishi, Naoki Yamaji, Jian Feng Ma

    The New phytologist   2023年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To play essential roles of manganese (Mn) in plant growth and development, it needs to be transported to different organs and tissues after uptake. However, the molecular mechanisms underlying Mn distribution between different tissues are poorly understood. We functionally characterized a member of rice natural resistance-associated macrophage protein (NRAMP) family, OsNramp5 in terms of its tissue specificity of gene expression, cell-specificity of protein localization, phenotypic analysis of leaf growth and response to Mn fluctuations. OsNramp5 is highly expressed in the leaf sheath. Immunostaining revealed that OsNramp5 is polarly localized at the proximal side of xylem parenchyma cells of the leaf sheath. Both the gene expression and protein abundance of OsNramp5 are unaffected by different Mn concentrations. Knockout of OsNramp5 decreased the distribution of Mn to the leaf sheath, but increased the distribution to the leaf blade at both low and high Mn supplies, resulting in reduced growth of leaf sheath. Furthermore, expression of OsNramp5 under the control of root-specific promoter in osnramp5 mutant complemented Mn uptake, but could not complement Mn distribution to the leaf sheath. These results indicate that OsNramp5 expressed in the leaf sheath plays an important role in unloading Mn from the xylem for the local distribution in rice.

    DOI: 10.1111/nph.19454

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  • Knockout of a rice K5.2 gene increases Ca accumulation in the grain. 国際誌

    Peitong Wang, Naoki Yamaji, Namiki Mitani-Ueno, Jun Ge, Jian Feng Ma

    Journal of integrative plant biology   2023年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Rice is a staple food for half world's population, but it is a poor dietary source of calcium (Ca) due to its low concentration. Therefore, it is an important issue to boost the Ca concentration in the grain for improving Ca deficiency risk, but the mechanisms underlying the Ca accumulation are poorly understood. Here, we obtained a rice (Oryza sativa) mutant with high shoot Ca accumulation. The mutant exhibited 26-53% higher Ca in the shoots than that in wild-type rice (WT) at different Ca supplies. The Ca concentration in the xylem sap was 36% higher in the mutant than in the WT. There was no difference in agronomic traits between the WT and mutant, but the mutant showed 25% higher Ca in the polished grain compared with the WT. Map-based cloning combined with complementation test revealed that the mutant phenotype was caused by an 18-bp deletion of a gene, OsK5.2 belonging to Shaker-like K+ channel family. OsK.2 was highly expressed in the mature region of the roots and its expression in the roots was not affected by Ca levels, but up-regulated by low K. Immunostaining showed that OsK.2 was mainly expressed in pericycle of the roots. Taken together, our results revealed a novel role of OsK.2 in Ca translocation in rice, which will be a good target for Ca biofortification in rice. This article is protected by copyright. All rights reserved.

    DOI: 10.1111/jipb.13587

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  • A silicon transporter gene required for healthy growth of rice on land. 国際誌

    Namiki Mitani-Ueno, Naoki Yamaji, Sheng Huang, Yuma Yoshioka, Takaaki Miyaji, Jian Feng Ma

    Nature communications   14 ( 1 )   6522 - 6522   2023年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Silicon (Si) is the most abundant mineral element in the earth's crust. Some plants actively accumulate Si as amorphous silica (phytoliths), which can protect plants from stresses. Here, we report a gene (SIET4) that is required for the proper accumulation and cell-specific deposition of Si in rice and show that it is essential for normal growth. SIET4 is constitutively expressed in leaves and encodes a Si transporter. SlET4 polarly localizes at the distal side of epidermal cells and cells surrounding the bulliform cells (motor cells) of the leaf blade, where Si is deposited. Knockout of SIET4 leads to the death of rice in the presence but not absence of Si. Further analysis shows that SIET4 knockout induces abnormal Si deposition in mesophyll cells and the induction of hundreds of genes related to various stress responses. These results indicate that SIET4 is required for the proper export of Si from leaf cells to the leaf surface and for the healthy growth of rice on land.

    DOI: 10.1038/s41467-023-42180-y

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  • A vacuolar transporter plays important roles in zinc and cadmium accumulation in rice grain. 国際誌

    Min Ning, Shi Jia Liu, Fenglin Deng, Liyu Huang, Hu Li, Jing Che, Naoki Yamaji, Fengyi Hu, Gui Jie Lei

    The New phytologist   239 ( 5 )   1919 - 1934   2023年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Rice grain is a poor dietary source of zinc (Zn) but the primary source of cadmium (Cd) for humans; however, the molecular mechanisms for their accumulation in rice grain remain incompletely understood. This study functionally characterized a tonoplast-localized transporter, OsMTP1. OsMTP1 was preferentially expressed in the roots, aleurone layer, and embryo of seeds. OsMTP1 knockout decreased Zn concentration in the root cell sap, roots, aleurone layer and embryo, and subsequently increased Zn concentration in shoots and polished rice (endosperm) without yield penalty. OsMTP1 haplotype analysis revealed elite alleles associated with increased Zn level in polished rice, mostly because of the decreased OsMTP1 transcripts. OsMTP1 expression in yeast enhanced Zn tolerance but did not affect that of Cd. While OsMTP1 knockout resulted in decreased uptake, translocation and accumulation of Cd in plant and rice grain, which could be attributed to the indirect effects of altered Zn accumulation. Our results suggest that rice OsMTP1 primarily functions as a tonoplast-localized transporter for sequestrating Zn into vacuole. OsMTP1 knockout elevated Zn concentration but prevented Cd deposition in polished rice without yield penalty. Thus, OsMTP1 is a candidate gene for enhancing Zn level and reducing Cd level in rice grains.

    DOI: 10.1111/nph.19070

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  • Polar localization of a rice silicon transporter requires isoleucine at both C- and N-termini as well as positively charged residues. 国際誌

    Noriyuki Konishi, Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    The Plant cell   35 ( 6 )   2232 - 2250   2023年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Silicon (Si) is important for stable and high yields in rice (Oryza sativa), a typical Si hyperaccumulator. The high Si accumulation is achieved by the cooperation of 2 Si transporters, LOW SILICON 1 (OsLsi1) and OsLsi2, which are polarly localized in cells of the root exodermis and endodermis. However, the mechanism underlying their polar localization is unknown. Here, we identified amino acid residues critical for the polar localization of OsLsi1. Deletion of both N- and C-terminal regions resulted in the loss of its polar localization. Furthermore, the deletion of the C-terminus inhibited its trafficking from the endoplasmic reticulum to the plasma membrane. Detailed site-directed mutagenesis analysis showed that Ile18 at the N-terminal region and Ile285 at the C-terminal region were essential for the polar localization of OsLsi1. Moreover, a cluster of positively charged residues at the C-terminal region is also required for polar localization. Phosphorylation and Lys modifications of OsLsi1 are unlikely to be involved in its polar localization. Finally, we showed that the polar localization of OsLsi1 is required for the efficient uptake of Si. Our study not only identified critical residues required for the polar localization of OsLsi1, but also provided experimental evidence for the importance of transporter polarity for efficient nutrient uptake.

    DOI: 10.1093/plcell/koad073

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  • FE UPTAKE-INDUCING PEPTIDE1 maintains Fe translocation by controlling Fe deficiency response genes in the vascular tissue of Arabidopsis. 国際誌

    Satoshi Okada, Gui J Lei, Naoki Yamaji, Sheng Huang, Jian F Ma, Keiichi Mochida, Takashi Hirayama

    Plant, cell & environment   45 ( 11 )   3322 - 3337   2022年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    FE UPTAKE-INDUCING PEPTIDE1 (FEP1), also named IRON MAN3 (IMA3) is a short peptide involved in the iron deficiency response in Arabidopsis thaliana. Recent studies uncovered its molecular function, but its physiological function in the systemic Fe response is not fully understood. To explore the physiological function of FEP1 in iron homoeostasis, we performed a transcriptome analysis using the FEP1 loss-of-function mutant fep1-1 and a transgenic line with oestrogen-inducible expression of FEP1. We determined that FEP1 specifically regulates several iron deficiency-responsive genes, indicating that FEP1 participates in iron translocation rather than iron uptake in roots. The iron concentration in xylem sap under iron-deficient conditions was lower in the fep1-1 mutant and higher in FEP1-induced transgenic plants compared with the wild type (WT). Perls staining revealed a greater accumulation of iron in the cortex of fep1-1 roots than in the WT root cortex, although total iron levels in roots were comparable in the two genotypes. Moreover, the fep1-1 mutation partially suppressed the iron overaccumulation phenotype in the leaves of the oligopeptide transporter3-2 (opt3-2) mutant. These data suggest that FEP1 plays a pivotal role in iron movement and in maintaining the iron quota in vascular tissues in Arabidopsis.

    DOI: 10.1111/pce.14424

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  • Linking root morphology and anatomy with transporters for mineral element uptake in plants

    Yu En, Naoki Yamaji, Jian Feng Ma

    Plant and Soil   2022年9月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s11104-022-05692-y

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  • Duplication of a manganese/cadmium transporter gene reduces cadmium accumulation in rice grain

    En Yu, Wenguang Wang, Naoki Yamaji, Shuichi Fukuoka, Jing Che, Daisei Ueno, Tsuyu Ando, Fenglin Deng, Kiyosumi Hori, Masahiro Yano, Ren Fang Shen, Jian Feng Ma

    Nature Food   2022年8月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s43016-022-00569-w

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  • A Golgi‐localized glycosyltransferase, OsGT14 ;1 is required for growth of both roots and shoots in rice

    Peitong Wang, Naoki Yamaji, Jian Feng Ma

    The Plant Journal   2022年7月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/tpj.15897

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  • NRAMP6 and NRAMP1 cooperatively regulate root growth and manganese translocation under manganese deficiency in Arabidopsis

    Lun Li, Zongzheng Zhu, Yonghui Liao, Changhong Yang, Ni Fan, Jie Zhang, Naoki Yamaji, Léon Dirick, Jian Feng Ma, Catherine Curie, Chao‐Feng Huang

    The Plant Journal   2022年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/tpj.15754

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  • Cell-Type-Dependent but CME-Independent Polar Localization of Silicon Transporters in Rice

    Noriyuki Konishi, Sheng Huang, Naoki Yamaji, Jian Feng Ma

    Plant and Cell Physiology   63 ( 5 )   699 - 712   2022年5月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press ({OUP})  

    DOI: 10.1093/pcp/pcac032

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  • A pericycle‐localized silicon transporter for efficient xylem loading in rice

    Sheng Huang, Naoki Yamaji, Gen Sakurai, Namiki Mitani‐Ueno, Noriyuki Konishi, Jian Feng Ma

    New Phytologist   234 ( 1 )   197 - 208   2022年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/nph.17959

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.17959

  • Effects of polygalacturonase overexpression on pectin distribution in the elongation zones of roots under aluminium stress. 国際誌

    Teruki Nagayama, Akane Tatsumi, Atsuko Nakamura, Naoki Yamaji, Shinobu Satoh, Jun Furukawa, Hiroaki Iwai

    AoB PLANTS   14 ( 2 )   plac003   2022年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The roots of many plant species contain large amounts of pectin and it contributes to the formation of the rhizosphere. In the present study, the relationship between the root-tip pectin content and aluminium (Al) tolerance in wild-type (WT) and demethylesterified pectin degradation enzyme gene overexpressor (OsPG2-FOX) rice lines was compared. OsPG2-FOX rice showed reduced pectin content in roots, even under control conditions; Al treatment reduced root elongation and the pectin content in the root elongation zone. Wild-type rice showed more pectin accumulation in the root elongation zone after Al treatment. Relative to WT rice, OsPG2-FOX rice showed more Al accumulation in the root elongation zone. These results indicate that the amount of pectin influences Al tolerance and that the distribution of pectin in the root elongation zone inhibits Al accumulation in rice roots. Pectin accumulation in cell walls in the root elongation zone may play a role in protecting rice plants from the Al-induced inhibition of root elongation by regulating pectin distribution.

    DOI: 10.1093/aobpla/plac003

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  • A crucial role for a node-localized transporter, HvSPDT, in loading phosphorus into barley grains. 国際誌

    Mian Gu, Hengliang Huang, Hiroshi Hisano, Guangda Ding, Sheng Huang, Namiki Mitani-Ueno, Kengo Yokosho, Kazuhiro Sato, Naoki Yamaji, Jian Feng Ma

    The New phytologist   2022年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Grains are the major sink of phosphorus (P) in cereal crops, accounting for 60-85% of total plant P, but the mechanisms underlying P loading into the grains are poorly understood. We functionally characterized a transporter gene required for the distribution of P to the grains in barley (Hordeum vulgare), HvSPDT (SULTR-like phosphorus distribution transporter). HvSPDT encoded a plasma membrane-localized Pi/H+ cotransporter. It was mainly expressed in the nodes at both the vegetative and reproductive stages. Furthermore, its expression was induced by inorganic phosphate (Pi) deficiency. In the nodes, HvSPDT was expressed in both the xylem and phloem region of enlarged and diffuse vascular bundles. Knockout of HvSPDT decreased the distribution of P to new leaves, but increased the distribution to old leaves at the vegetative growth stage under low P supply. However, knockout of HvSPDT did not alter the redistribution of P from old to young organs. At the reproductive stage, knockout of HvSPDT significantly decreased P allocation to the grains, resulting in a considerable reduction in grain yield, especially under P-limited conditions. Our results indicate that node-based HvSPDT plays a crucial role in loading P into barley grains through preferentially distributing P from the xylem and further to the phloem.

    DOI: 10.1111/nph.18057

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  • Boron uptake in rice is regulated post-translationally via a clathrin-independent pathway

    Sheng Huang, Noriyuki Konishi, Naoki Yamaji, Ji Feng Shao, Namiki Mitani-Ueno, Jian Feng Ma

    Plant Physiology   188 ( 3 )   1649 - 1664   2021年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    <title>Abstract</title>
    Uptake of boron (B) in rice (Oryza sativa) is mediated by the Low silicon rice 1 (OsLsi1) channel, belonging to the NOD26-like intrinsic protein III subgroup, and the efflux transporter B transporter 1 (OsBOR1). However, it is unknown how these transporters cooperate for B uptake and how they are regulated in response to B fluctuations. Here, we examined the response of these two transporters to environmental B changes at the transcriptional and posttranslational level. OsBOR1 showed polar localization at the proximal side of both the exodermis and endodermis of mature root region, forming an efficient uptake system with OsLsi1 polarly localized at the distal side of the same cell layers. Expression of OsBOR1 and OsLsi1 was unaffected by B deficiency and excess. However, although OsLsi1 protein did not respond to high B at the protein level, OsBOR1 was degraded in response to high B within hours, which was accompanied with a significant decrease of total B uptake. The high B-induced degradation of OsBOR1 was inhibited in the presence of MG-132, a proteasome inhibitor, without disturbance of the polar localization. In contrast, neither the high B-induced degradation of OsBOR1 nor its polarity was affected by induced expression of dominant-negative mutated dynamin-related protein 1A (OsDRP1AK47A) or knockout of the mu subunit (AP2M) of adaptor protein-2 complex, suggesting that clathrin-mediated endocytosis is not involved in OsBOR1 degradation and polar localization. These results indicate that, in contrast to Arabidopsis thaliana, rice has a distinct regulatory mechanism for B uptake through clathrin-independent degradation of OsBOR1 in response to high B.

    DOI: 10.1093/plphys/kiab575

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  • Metalloid transporters and their regulation in plants

    Naoki Yamaji, Jian Feng Ma

    Plant Physiology   187 ( 4 )   1929 - 1939   2021年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press ({OUP})  

    DOI: 10.1093/plphys/kiab326

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  • Zinc Transport in Rice: Dilemma between optimal plant requirement and human nutrition. 国際誌

    Sheng Huang, Naoki Yamaji, Jian Feng Ma

    Journal of experimental botany   2021年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Zinc (Zn) is an essential micronutrient for both plants and animals, while Zn deficiency in crops and humans is a global problem affecting crop productivity and human health. Since plants and humans differ in their Zn requirement, there is a dilemma between plant nutrition and human nutrition. In this review, we focus on the transport system of Zn from soil to grain in rice (Oryza sativa), which is a major dietary source of Zn for people subsiding on rice-based diets. We describe transporters belonging to different family, which are involved in the uptake, vacuolar sequestration, root-to-shoot translocation and distribution of Zn. We also discuss the possible regulation mechanism of these transporters. Several examples are given on enhancing Zn accumulation and bioavailability in rice grains through manipulation of genes highly expressed in the nodes, where Zn is highly deposited. We finally provide our perspectives toward breeding rice cultivars with both increased tolerance to Zn-deficiency stress and high Zn density in rice grains.

    DOI: 10.1093/jxb/erab478

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  • Structural basis for high selectivity of a rice silicon channel Lsi1. 国際誌

    Yasunori Saitoh, Namiki Mitani-Ueno, Keisuke Saito, Kengo Matsuki, Sheng Huang, Lingli Yang, Naoki Yamaji, Hiroshi Ishikita, Jian-Ren Shen, Jian Feng Ma, Michihiro Suga

    Nature communications   12 ( 1 )   6236 - 6236   2021年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Silicon (Si), the most abundant mineral element in the earth's crust, is taken up by plant roots in the form of silicic acid through Low silicon rice 1 (Lsi1). Lsi1 belongs to the Nodulin 26-like intrinsic protein subfamily in aquaporin and shows high selectivity for silicic acid. To uncover the structural basis for this high selectivity, here we show the crystal structure of the rice Lsi1 at a resolution of 1.8 Å. The structure reveals transmembrane helical orientations different from other aquaporins, characterized by a unique, widely opened, and hydrophilic selectivity filter (SF) composed of five residues. Our structural, functional, and theoretical investigations provide a solid structural basis for the Si uptake mechanism in plants, which will contribute to secure and sustainable rice production by manipulating Lsi1 selectivity for different metalloids.

    DOI: 10.1038/s41467-021-26535-x

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  • Lateral roots but not root hairs contribute to high uptake of manganese and cadmium in rice. 国際誌

    En Yu, Naoki Yamaji, Chuanzao Mao, Hua Wang, Jian Feng Ma

    Journal of experimental botany   72 ( 20 )   7219 - 7228   2021年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Rice (Oryza sativa L.) can accumulate high manganese (Mn) in the shoots through uptake by the roots, which consist of crown roots, lateral roots and root hairs. We investigated the role of lateral roots and root hairs in Mn and cadmium (Cd) uptake by using two indica rice mutants defective in formation of lateral roots (osiaa11) and root hairs (osrhl1). The uptake of Mn and Cd in osiaa11 was significantly lower than that in wild type 'Kasalath', but there was no difference between wild type and osrhl1. Furthermore, a kinetic study showed that Mn uptake in osiaa11 was much lower than that in wild type and osrhl1 across a wide range of Mn concentrations. The role of lateral roots in Mn and Cd uptake was further confirmed in a japonica rice mutant defective in lateral root formation. We found that expression of Mn transporter gene Natural Resistance-Associated Macrophage Protein 5 (OsNRAMP5), but not of Metal Tolerance Protein 9 (OsMTP9), was lower in osiaa11 than in wild type; however, there were no differences between osrhl1 and the wild type. Immunostaining showed that OsNRAMP5 and OsMTP9 were localized in the exodermis and endodermis of crown roots and lateral roots, but not in the root hairs. Taken together, our results indicate that lateral roots, but not root hairs, play an important role in high Mn and Cd uptake in rice.

    DOI: 10.1093/jxb/erab329

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  • Effectiveness of Create ML in microscopy image classifications: a simple and inexpensive deep learning pipeline for non-data scientists. 国際誌

    Kiyotaka Nagaki, Tomoyuki Furuta, Naoki Yamaji, Daichi Kuniyoshi, Megumi Ishihara, Yuji Kishima, Minoru Murata, Atsushi Hoshino, Hirotomo Takatsuka

    Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology   29 ( 3-4 )   361 - 371   2021年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Observing chromosomes is a time-consuming and labor-intensive process, and chromosomes have been analyzed manually for many years. In the last decade, automated acquisition systems for microscopic images have advanced dramatically due to advances in their controlling computer systems, and nowadays, it is possible to automatically acquire sets of tiling-images consisting of large number, more than 1000, of images from large areas of specimens. However, there has been no simple and inexpensive system to efficiently select images containing mitotic cells among these images. In this paper, a classification system of chromosomal images by deep learning artificial intelligence (AI) that can be easily handled by non-data scientists was applied. With this system, models suitable for our own samples could be easily built on a Macintosh computer with Create ML. As examples, models constructed by learning using chromosome images derived from various plant species were able to classify images containing mitotic cells among samples from plant species not used for learning in addition to samples from the species used. The system also worked for cells in tissue sections and tetrads. Since this system is inexpensive and can be easily trained via deep learning using scientists' own samples, it can be used not only for chromosomal image analysis but also for analysis of other biology-related images.

    DOI: 10.1007/s10577-021-09676-z

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  • Buckwheat FeNramp5 Mediates High Manganese Uptake in Roots.

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    Plant & cell physiology   62 ( 4 )   600 - 609   2021年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Manganese (Mn) is an essential element for plant growth and development, but transporters required for Mn uptake have only been identified in a few plant species. Here, we functionally characterized a member of the natural resistance-associated macrophage proteins (Nramps) family, FeNramp5 in buckwheat (Fagopyrum esculentum Moench), which is known as a species well adapted to acidic soils. FeNramp5 was mainly expressed in the roots, and its expression was upregulated by the deficiency of Mn and Fe. Furthermore, spatial and tissue-specific expression analysis showed that FeNramp5 was expressed in all tissues of the basal root regions. FeNramp5-GFP protein was localized to the plasma membrane when transiently expressed in buckwheat leaf protoplast. FeNramp5 showed the transport activity for Mn2+ and Cd2+ but not for Fe2+ when expressed in yeast. Furthermore, the transport activity for Mn2+ was higher in yeast expressing FeNramp5 than in yeast expressing AtNramp1. FeNramp5 was also able to complement the phenotype of Arabidopsis atnramp1 mutant in terms of the growth and accumulation of Mn and Cd. The absolute expression level of AtNramp1 was comparable to that of FeNramp5 in the roots, but buckwheat accumulated higher Mn than Arabidopsis when grown under the same condition. Further analysis showed that at least motif B in FeNramp5 seems important for its high transport activity for Mn. These results indicate that FeNramp5 is a transporter for the uptake of Mn and Cd and its higher transport activity for Mn is probably associated with higher Mn accumulation in buckwheat.

    DOI: 10.1093/pcp/pcaa153

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  • Lysine Ketoglutarate Reductase Trans-Splicing Related 1 Is Involved In A Temperature-Dependent Root Growth in Rice. 国際誌

    En Yu, Naoki Yamaji, Keiich Mochida, Ivan Galis, Kanatani Asaka, Jian Feng Ma

    Journal of experimental botany   72 ( 18 )   6336 - 6349   2021年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Root length is one of the important parameters of root traits directly related to uptake of water and nutrients, however, the molecular mechanisms controlling root length are still not fully understood. Here, we isolated a mutant, dice2 (defective in cell elongation 2) of rice (Oryza sativa) showing short roots. The cell length and meristem size of the roots was decreased in the mutant, but the root function in mineral element uptake normalized by root biomass, root cell width and root anatomy was hardly altered compared with the wild-type rice (WT). Intriguingly, the root growth defect in the mutant could be partially rescued by high temperature. The mutant accumulated more H2O2 and glutamic acid, but less O2  •- in the roots at low temperature than WT, but they became similar at high temperature between two lines. A map-based cloning combined with complementation test revealed that the short-root phenotype was caused by a nucleotide substitution of a gene, which was annotated to encode Lysine Ketoglutarate Reductase Trans-Splicing related 1 (OsLKRT1). OsLKRT1 encodes a cytosol-localized protein and was expressed in both the roots and shoots. Furthermore, OsLKRT1 was expressed in all cells of the root tip and root elongation regions. RNA-seq analysis showed that there was no difference in the expression level of genes involved in root development identified so far. These results indicate that the gene identified in this study is involved in novel pathway required for cell elongation of the roots in rice although its exact role remains to be further investigated.

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  • Role of a vacuolar iron transporter OsVIT2 in the distribution of iron to rice grains

    Jing Che, Naoki Yamaji, Jian Feng Ma

    NEW PHYTOLOGIST   230 ( 3 )   1049 - 1062   2021年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Iron (Fe) from rice grains is an important source of dietary intake; however, the molecular mechanisms responsible for loading of Fe to the grains are poorly understood.We functionally characterized a vacuolar iron transporter gene, OsVIT2 in terms of expression pattern, cellular localization, and mutant phenotypes.OsVIT2 was expressed in the parenchyma cell bridges of nodes, in the mestome sheath of leaf sheath and aleurone of the caryopsis. Mutation of OsVIT2 resulted in decreased Fe distribution to the leaf sheath, nodes, and aleurone, but increased Fe to the leaf blade and grains. Furthermore, Fe was heavily deposited in the parenchyma cell bridges, mestome sheath and aleurone in the wild-type rice, but this accumulation was decreased in the knockout lines. Conversely, heavier deposition of Fe was observed in the embryo and endosperm of the grains of knockout lines compared with the wild-type rice, resulting in increased Fe accumulation in the polished rice without yield penalty.These results indicate that OsVIT2 is involved in the distribution of Fe to the grains through sequestering Fe into vacuoles in mestome sheath, nodes, and aleurone layer and that knockout of this gene provides a potential way for Fe biofortification without yield penalty.

    DOI: 10.1111/nph.17219

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  • Fine regulation system for distribution of boron to different tissues in rice 国際誌

    Ji Feng Shao, Naoki Yamaji, Sheng Huang, Jian Feng Ma

    NEW PHYTOLOGIST   230 ( 2 )   656 - 668   2021年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Boron (B) is essential for growth and development, with the B requirement differing depending on the particular organs and tissues, but the molecular mechanisms underlying the preferential distribution of B to different tissues are poorly understood.We investigated the role of a rice gene (OsBOR1) encoding a B efflux transporter in the distribution of B to different tissues under different B supplies.OsBOR1 was highly expressed in the nodes at all growth stages. The OsBOR1 protein shows polar localization at the distal side of bundle sheath cells in nodes and xylem parenchyma cells of elongating leaf sheath, but in the mature leaf sheath and blade at the proximal side of bundle sheath cells. Furthermore, the expression of OsBOR1 was not affected by external B fluctuations, but the OsBOR1 protein was gradually degraded in response to high B. Knockout of this gene altered B distribution, decreasing the distribution of B to new leaves and panicles but increasing B distribution to old leaves.These results indicate that OsBOR1 expressed in nodes and leaf sheath is involved in the preferential distribution of B to different tissues in rice. Furthermore, the OsBOR1 undergoes degradation in response to high B for fine regulation of B distribution to different tissues.

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  • A transporter for delivering zinc to the developing tiller bud and panicle in rice

    Shuai Mu, Naoki Yamaji, Akimasa Sasaki, Le Luo, Binbin Du, Jing Che, Huichao Shi, Haoqiang Zhao, Sheng Huang, Fenglin Deng, Zhenguo Shen, Mary Lou Guerinot, Luqing Zheng, Jian Feng Ma

    PLANT JOURNAL   105 ( 3 )   786 - 799   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Tiller number is one of the most important agronomic traits that determine rice (Oryza sativa) yield. Active growth of tiller bud (TB) requires high amount of mineral nutrients; however, the mechanism underlying the distribution of mineral nutrients to TB with low transpiration is unknown. Here, we found that the distribution of Zn to TB is mediated by OsZIP4, one of the ZIP (ZRT, IRT-like protein) family members. The expression of OsZIP4 was highly detected in TB and nodes, and was induced by Zn deficiency. Immunostaining analysis revealed that OsZIP4 was mainly expressed in phloem of diffuse vascular bundles in the nodes and the axillary meristem. The mutation of OsZIP4 did not affect the total Zn uptake, but altered Zn distribution; less Zn was delivered to TB and new leaf, but more Zn was retained in the basal stems at the vegetative growth stage. Bioimaging analysis showed that the mutant aberrantly accumulated Zn in enlarged and transit vascular bundles of the basal node, whereas in wild-type high accumulation of Zn was observed in the meristem part. At the reproductive stage, mutation of OsZIP4 resulted in delayed panicle development, which is associated with decreased Zn distribution to the panicles. Collectively, OsZIP4 is involved in transporting Zn to the phloem of diffuse vascular bundles in the nodes for subsequent distribution to TBs and other developing tissues. It also plays a role in transporting Zn to meristem cells in the TBs.

    DOI: 10.1111/tpj.15073

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  • Two metallothionein genes highly expressed in rice nodes are involved in distribution of Zn to the grain

    Gui Jie Lei, Naoki Yamaji, Jian Feng Ma

    NEW PHYTOLOGIST   229 ( 2 )   1007 - 1020   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    A rice node is a hub for distribution of mineral elements; however, most genes highly expressed in the node have not been functionally characterized. Transcriptomic analysis of a rice node revealed that two metallothionein genes,OsMT2bandOsMT2c, were highly expressed in the node I. We functionally characterized these genes in terms of gene expression pattern, cellular and subcellular localization, phenotypic analysis of the single and double knockout mutants and metal-binding ability. BothOsMT2bandOsMT2cwere mainly and constitutively expressed in the phloem region of enlarged and diffuse vascular bundles in the nodes and of the anther. Knockout of eitherOsMT2borOsMT2cincreased zinc (Zn) accumulation in the nodes, but decreased Zn distribution to the panicle, resulting in decreased grain yield. A double mutant,osmt2bmt2c, showed further negative effects on the Zn distribution and grain yield. By contrast, knockout ofOsMT2bhad a small effect on copper (Cu) accumulation. Both OsMT2b and OsMT2c showed binding ability with Zn, whereas only OsMT2b showed binding ability with Cu in yeast. Our results suggest that bothOsMT2bandOsMT2cplay an important role mainly in the distribution of Zn to grain through chelation and subsequent transport of Zn in the phloem in rice.

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  • OsNRAMP1transporter contributes to cadmium and manganese uptake in rice

    Jia-Dong Chang, Sheng Huang, Naoki Yamaji, Wenwen Zhang, Jian Feng Ma, Fang-Jie Zhao

    PLANT CELL AND ENVIRONMENT   43 ( 10 )   2476 - 2491   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Rice is a major dietary source of the toxic metal, cadmium (Cd). Previous studies reported that the rice transporter, OsNRAMP1, (Natural resistance-associated macrophage protein 1) could transport iron (Fe), Cd and arsenic (As) in heterologous yeast assays. However, thein plantafunction of OsNRAMP1 remains unknown. Here, we showed that OsNRAMP1 was able to transport Cd and manganese (Mn) when expressed in yeast, but did not transport Fe or As.OsNRAMP1was mainly expressed in roots and leaves and encoded a plasma membrane-localized protein.OsNRAMP1expression was induced by Cd treatment and Fe deficiency. Immunostaining showed that OsNRAMP1 was localized in all root cells, except the central vasculature, and in leaf mesophyll cells. The knockout ofOsNRAMP1resulted in significant decreases in root uptake of Cd and Mn and their accumulation in rice shoots and grains, and increased sensitivity to Mn deficiency. The knockout ofOsNRAMP1had smaller effects on Cd and Mn uptake than knockout ofOsNRAMP5, while knockout of both genes resulted in large decreases in the uptake of the two metals. Taken together, OsNRAMP1 contributes significantly to the uptake of Mn and Cd in rice, and the functions of OsNRAMP1 and OsNRAMP5 are similar but not redundant.

    DOI: 10.1111/pce.13843

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  • イネ種子の発育に必要な節局在性のリン酸輸送体

    CHE Jing, CHE Jing, 山地直樹, 宮地孝明, 三谷奈見季, 加藤百合, SHEN Ren Fang, MA Jian Feng

    日本植物生理学会年会(Web)   61 ( 8 )   1387 - 1398   2020年8月

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  • Breeding for low cadmium barley by introgression of a Sukkula-like transposable element

    Gui Jie Lei, Miho Fujii-Kashino, De Zhi Wu, Hiroshi Hisano, Daisuke Saisho, Fenglin Deng, Naoki Yamaji, Kazuhiro Sato, Fang-Jie Zhao, Jian Feng Ma

    Nature Food   1 ( 8 )   489 - 499   2020年8月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1038/s43016-020-0130-x

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  • The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions(1)([OPEN])

    Sheng Huang, Akimasa Sasaki, Naoki Yamaji, Haruka Okada, Namiki Mitani-Ueno, Jian Feng Ma

    PLANT PHYSIOLOGY   183 ( 3 )   1224 - 1234   2020年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS INC  

    Zinc (Zn) is an important essential micronutrient for plants and humans; however, the exact transporter responsible for root zinc uptake from soil has not been identified. Here, we found that OsZIP9, a member of the ZRT-IRT-related protein, is involved in Zn uptake in rice (Oryza sativa) under Zn-limited conditions. OsZIP9 was mainly localized to the plasma membrane and showed transport activity for Zn in yeast (Saccharomyces cerevisiae). Expression pattern analysis showed thatOsZIP9was mainly expressed in the roots throughout all growth stages and its expression was upregulated by Zn-deficiency. Furthermore,OsZIP9was expressed in the exodermis and endodermis of root mature regions. For plants grown in a hydroponic solution with low Zn concentration, knockout ofOsZIP9significantly reduced plant growth, which was accompanied by decreased Zn concentrations in both the root and shoot. However, plant growth and Zn accumulation did not differ between knockout lines and wild-type rice under Zn-sufficient conditions. When grown in soil, Zn concentrations in the shoots and grains of knockout lines were decreased to half of wild-type rice, whereas the concentrations of other mineral nutrients were not altered. A short-term kinetic experiment with stable isotope(67)Zn showed that(67)Zn uptake in knockout lines was much lower than that in wild-type rice. Combined, these results indicate that OsZIP9 localized at the root exodermis and endodermis functions as an influx transporter of Zn and contributes to Zn uptake under Zn-limited conditions in rice.The zinc transporter OsZIP9, expressed at the exodermis and endodermis of root mature region, contributes to Zn uptake from soil in rice.

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  • Diel magnesium fluctuations in chloroplasts contribute to photosynthesis in rice

    Jian Li, Kengo Yokosho, Sheng Liu, Hong Rui Cao, Naoki Yamaji, Xin Guang Zhu, Hong Liao, Jian Feng Ma, Zhi Chang Chen

    NATURE PLANTS   6 ( 7 )   848 - +   2020年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE RESEARCH  

    Photosynthesis in plants responds to light fluctuations and displays circadian rhythms in various aspects. Here a new regulator of photosynthetic rhythms is identified in rice and shown to control diurnal magnesium fluctuations in the chloroplast. Further engineering attempts yield enhanced rice growth and photosynthesis.Photosynthesis provides food, fibre and fuel that support our society; understanding the mechanisms controlling dynamic changes in this process helps identify new options to improve photosynthesis. Photosynthesis shows diel changes, which have been largely attributed to external light/dark conditions, as well as internal gene expression and the post-translational modification of critical enzymes. Here we report diel fluctuations of magnesium (Mg) in rice (Oryza sativa) chloroplasts, which may function as a rhythm regulator contributing to the post-translational regulation of photosynthetic CO(2)assimilation in rice. We found that a chloroplast-localized Mg(2+)transporter gene,OsMGT3, which is rhythmically expressed in leaf mesophyll cells, partly modulates Mg fluctuations in rice chloroplasts. Knockout ofOsMGT3substantially reduced Mg(2+)uptake, as well as the amplitude of free Mg(2+)fluctuations in chloroplasts, which was closely associated with a decrease in ribulose 1,5-bisphosphate carboxylase activity in vivo and a consequent decline in the photosynthetic rate. In addition, the mesophyll-specific overexpression ofOsMGT3remarkably improved photosynthetic efficiency and growth performance in rice. Taken together, these observations demonstrate that OsMGT3-dependent diel Mg fluctuations in chloroplasts may contribute to Mg-dependent enzyme activities for photosynthesis over the daily cycle. Enhancing Mg(2+)input to chloroplasts could be a potential approach to improving photosynthetic efficiency in plants.

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  • Plastic transport systems of rice for mineral elements in response to diverse soil environmental changes

    Peitong Wang, Naoki Yamaji, Komaki Inoue, Keiich Mochida, Jian Feng Ma

    NEW PHYTOLOGIST   226 ( 1 )   156 - 169   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Climate change will increase frequency of drought and flooding, which threaten global crop productivity and food security. Rice (Oryza sativa) is unique in that it is able to grow in both flooded and upland conditions, which have large differences in the concentrations and chemical forms of mineral elements available to plants. To comprehensively understand the mechanisms of rice for coping with different water status, we performed ionomics and transcriptomics analysis of the roots, nodes and leaves of rice grown in flooded and upland conditions. Focusing the analysis on genes encoding proteins involved in transport functions for mineral elements, it was found that, although rice plants maintained similar levels of each element in the shoots for optimal growth, different transporters for mineral elements were utilised for nitrogen, iron, copper and zinc to deal with different soil water conditions. For example, under flooded conditions, rice roots take up nitrogen using transporters for both ammonium (OsAMT1/2) and nitrate (OsNPF2.4, OsNRT1.1A and OsNRT2.3), whereas under upland conditions, nitrogen uptake is mediated by different nitrate transporters (OsNRT1.1B and OsNRT1.5A). This study shows that rice possesses plastic transport systems for mineral elements in response to different water conditions (upland and flooding).

    DOI: 10.1111/nph.16335

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  • Altered Root Structure Affects Both Expression and Cellular Localization of Transporters for Mineral Element Uptake in Rice

    En Yu, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   61 ( 3 )   481 - 491   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    One of the most important roles of plant roots is to take up mineral elements for their growth. Although several genes involved in root growth have been identified, the association between root structure and mineral element uptake is less investigated. In this study, we isolated a rice mutant (dice1, defective in cell elongation 1) with short-root phenotype. This mutant was characterized by partial defect in the formation of root outer cell layers. Mapping of the responsible gene revealed that the short-root phenotype in the mutant was caused by a single-nucleotide substitution of a gene encoding a membrane-anchored endo-1,4-beta-glucanase (OsGlu3). The growth of both the roots and shoots was partially recovered with increasing strength of nutrient solution and glucose in the mutant. The mutant showed a decreased uptake (normalized by root dry weight) for Mg, Mn, Fe, Cu, Zn, Cd, As and Ge but increased uptake for K and Ca. The expression level of some transporter genes including OsLsi1 and OsLsi2 for Si uptake and OsNromp5 for Mn uptake was significantly decreased in the mutant compared with the wild-type (WT) rice. Furthermore, the cellular localization of OsLsi1 was altered; OsLsi1 localized at the root exodermis of the WT rice was changed to be localized to other cell layers of the mutant roots. However, this localization became normal in the presence of exogenous glucose in the mutant. Our results indicate that a normal root structure is required for maintaining the expression and localization of transporters involved in the mineral element uptake.

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  • Decrosslinking enables visualization of RNA-guided endonuclease?in situ labeling signals for DNA sequences in plant tissues. 査読

    Kiyotaka Nagaki, Naoki Yamaji

    Journal of Experimental Botany   71 ( 6 )   1792 - 1800   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/jxb/erz534

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  • A transcription factor OsbHLH156 regulates Strategy II iron acquisition through localising IRO2 to the nucleus in rice

    Shoudong Wang, Lin Li, Yinghui Ying, Jin Wang, Ji Feng Shao, Naoki Yamaji, James Whelan, Jian Feng Ma, Huixia Shou

    NEW PHYTOLOGIST   225 ( 3 )   1247 - 1260   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Plants have evolved two strategies to acquire ferrous (Strategy I) or ferric (Strategy II) iron from soil. The iron-related bHLH transcription factor 2 (IRO2) has been identified as a key regulator of iron acquisition (Strategy II) in rice. However, its mode of action, subcellular localisation and binding partners are not clearly defined. Using RNA-seq analyses, we identified a novel bHLH-type transcription factor, OsbHLH156. The function of OsbHLH156 in Fe homeostasis was analysed by characterisation of the phenotypes, elemental content, transcriptome, interaction and subcellular localisation of OsbHLH156 and IRO2. OsbHLH156 is primarily expressed in the roots and transcript abundance is greatly increased by Fe deficiency. Loss of function of OsbHLH156 resulted in Fe-deficiency-induced chlorosis and reduced Fe concentration in the shoots under upland or Fe(III) supplied conditions. Transcriptome analyses revealed that the expression of most Fe-deficiency-responsive genes involved in Strategy II were not induced in the osbhlh156-1 mutant. Furthermore, OsbHLH156 was required for nuclear localisation of IRO2. We conclude that OsbHLH156 is required for a Strategy II uptake mechanism in rice, partnering with a previously identified 'master' regulator IRO2. Mechanistically it is required for the nuclear localisation of IRO2.

    DOI: 10.1111/nph.16232

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  • Vascular Cambium-Localized AtSPDT Mediates Xylem-to-Phloem Transfer of Phosphorus for Its Preferential Distribution in Arabidopsis

    Guangda Ding, Gui Jie Lei, Naoki Yamaji, Kengo Yokosho, Namiki Mitani-Ueno, Sheng Huang, Jian Feng Ma

    MOLECULAR PLANT   13 ( 1 )   99 - 111   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CELL PRESS  

    During plant growth and development mineral elements are preferentially delivered to different organs and tissues to meet the differential demand. It has been shown that the preferential distribution of mineral nutrients in gramineous plants is mediated by node-based transporters, but the mechanisms of preferential distribution in dicots are poorly understood. Here, we report a distinct mechanism for the preferential distribution of phosphorus (P) in Arabidopsis plants, revealed by detailed functional analysis of AtSPDT/AtSULTR3;4 (SULTR-like P Distribution Transporter), a homolog of rice OsSPDT. Like OsSPDT, AtSPDT is localized at the plasma membrane and showed proton-dependent transport activity for P. Interestingly, we found that AtSPDT is mainly expressed in the rosette basal region and leaf petiole, and its expression is up-regulated by P deficiency. Tissue-specific analysis showed that AtSPDT is mainly located in the vascular cambium of different organs, as well as in the parenchyma tissues of both xylem and phloem regions. Knockout of AtSPDT inhibited the growth of new leaves under low P due to decreased P distribution to those organs. The seed yields of the wild-type and atspdt mutant plants are similar, but the seeds of mutant plants contain - less P. These results indicate that AtSPDT localized in the vascular cambium is involved in preferential distribution of P to the developing tissues, through xylem-to-phloem transfer mainly at the rosette basal region and leaf petiole.

    DOI: 10.1016/j.molp.2019.10.002

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  • OsCASP1 Is Required for Casparian Strip Formation at Endodermal Cells of Rice Roots for Selective Uptake of Mineral Elements

    Zhigang Wang, Naoki Yamaji, Sheng Huang, Xiang Zhang, Mingxing Shi, Shan Fu, Guangzhe Yang, Jian Feng, Jixing Xia

    PLANT CELL   31 ( 11 )   2636 - 2648   2019年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS INC  

    In response to diverse environmental conditions, rice (Oryza sativa) roots have developed one Casparian strip (CS) at the exodermis and one CS at the endodermis. Here, we functionally characterized OsCASP1 (Casparian strip domain protein 1) in rice. OsCASP1 was mainly expressed in the root elongation zone, and the protein encoded was first localized to all sides of the plasma membrane of endodermal cells without CS, followed by the middle of the anticlinal side of endodermal cells with CS. Knockout of OsCASP1 resulted in a defect of CS formation at the endodermis and decreased growth under both soil and hydroponic conditions. Mineral analysis showed that the oscasp1 mutants accumulated more Ca, but less Mn, Zn, Fe, Cd, and As in the shoots compared with the wild type. The growth inhibition of the mutants was further aggravated by high Ca in growth medium. The polar localization of the Si transporter Low Si 1 at the distal side of the endodermis was not altered in the mutant, but the protein abundance was decreased, resulting in a substantial reduction in silicon uptake. These results indicated that OsCASP1 is required for CS formation at the endodermis and that the CS in rice plays an important role in root selective uptake of mineral elements, especially Ca and Si.

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  • A Vacuolar Phytosiderophore Transporter Alters Iron and Zinc Accumulation in Polished Rice Grains

    Jing Che, Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    PLANT PHYSIOLOGY   181 ( 1 )   276 - 288   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS INC  

    Essential metals, such as iron (Fe) and zinc (Zn), in grains are important sources for seed germination and nutritional requirements, but the molecular mechanisms underlying their loading into grains are poorly understood. Recently, nodes in rice (Oryza sativa) were reported to play an important role in the preferential distribution of mineral elements to the grains. In this study, we functionally characterized a rice gene highly expressed in nodes, OsVMT (VACUOLAR MUGINEIC ACID TRANSPORTER), belonging to a major facilitator superfamily. OsVMT is highly expressed in the parenchyma cell bridges of node I, where Fe and Zn are highly deposited. The expression of OsVMT was induced by Fe deficiency in the roots but not in the shoot basal region and uppermost node. OsVMT localized to the tonoplast and showed efflux transport activity for 2'-deoxymugineic acid (DMA). At the vegetative stage, knockout of OsVMT resulted in decreased DMA but increased ferric Fe in the root cell sap. As a result, the concentration of DMA in the xylem sap increased but that of ferric Fe decreased in the xylem sap in the mutants. In the polished rice grain, the mutants accumulated 1.8- to 2.1-fold, 1.5- to 1.6-fold, and 1.4- to 1.5-fold higher Fe, Zn, and DMA, respectively, than the wild type. Taken together, our results indicate that OsVMT is involved in sequestering DMA into the vacuoles and that knockout of this gene enhances the accumulation of Fe and Zn in polished rice grains through DMA-increased solubilization of Fe and Zn deposited in the node.

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  • Bioimaging of multiple elements by high‐resolution LA ‐ ICP ‐ MS reveals altered distribution of mineral elements in the nodes of rice mutants 査読

    Naoki Yamaji, Jian Feng Ma

    The Plant Journal   99 ( 6 )   1254 - 1263   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/tpj.14410

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  • The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice

    Hongmei Cai, Sheng Huang, Jing Che, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   70 ( 10 )   2717 - 2725   2019年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    In order to respond to fluctuating zinc (Zn) in the environment, plants must have a system to control Zn homeostasis. However, how plants maintain an appropriate level of Zn during their growth and development is still poorly understood. In this study, we found that OsHMA3, a tonoplast-localized transporter for Zn/Cd, plays an important role in Zn homeostasis in rice. Accessions with the functional allele of OsHMA3 showed greater tolerance to high Zn than those with the non-functional allele based on root elongation test. A 67Zn-labeling experiment showed that accessions with loss of function of OsHMA3 had lower Zn accumulation in the roots but similar concentrations in the shoots compared with functional OsHMA3 accessions. When exposed to Zn-free growing medium, the concentration in the root cell sap was rapidly decreased in accessions with functional OsHMA3, but less dramatic changes were observed in non-functional accessions. A mobility experiment showed that more Zn in the roots was translocated to the shoots in accessions with functional OsHMA3. Higher expression levels of OsZIP4, OsZIP5, OsZIP8, and OsZIP10 were found in the roots of accessions with functional OsHMA3 in response to Zn deficiency. Taken together, our results indicate that OsHMA3 plays an important role in rice roots in both Zn detoxification and storage by sequestration into the vacuoles, depending on Zn concentration in the environment.

    DOI: 10.1093/jxb/erz091

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  • A nodule‐localized phosphate transporter Gm PT 7 plays an important role in enhancing symbiotic N 2 fixation and yield in soybean 査読

    Liyu Chen, Lu Qin, Lili Zhou, Xinxin Li, Zhichang Chen, Lili Sun, Wenfei Wang, Zhihao Lin, Jing Zhao, Naoki Yamaji, Jian Feng Ma, Mian Gu, Guohua Xu, Hong Liao

    New Phytologist   221 ( 4 )   2013 - 2025   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/nph.15541

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  • OsPHT1;3 Mediates Uptake, Translocation, and Remobilization of Phosphate under Extremely Low Phosphate Regimes 査読

    Ming Xing Chang, Mian Gu, Yu Wei Xia, Xiao Li Dai, Chang Rong Dai, Jun Zhang, Shi Chao Wang, Hong Ye Qu, Naoki Yamaji, Jian Feng Ma, Guo Hua Xu

    Plant Physiology   179 ( 2 )   656 - 670   2019年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society of Plant Biologists ({ASPB})  

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  • Retrotransposon Insertion and DNA Methylation Regulate Aluminum Tolerance in European Barley Accessions. 査読 国際誌

    Miho Kashino-Fujii, Kengo Yokosho, Naoki Yamaji, Miki Yamane, Daisuke Saisho, Kazuhiro Sato, Jian Feng Ma

    Plant physiology   178 ( 2 )   716 - 727   2018年10月

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    記述言語:英語  

    Aluminum (Al) toxicity is a major stress factor limiting crop productivity in acid soil. Although there is great genotypic variation in tolerance to Al toxicity, the underlying molecular mechanisms are poorly understood. Here, we report that, in barley (Hordeum vulgare), the fourth largest cereal crop produced in the world, both retrotransposon insertion and DNA methylation are involved in regulating differential Al tolerance. HvAACT1 is a major gene responsible for citrate secretion from the roots for external detoxification of Al. A multiretrotransposon-like (MRL) sequence insertion at least 15.3 kb in length was detected in the upstream genomic region of HvAACT1 that displayed promoter activity and significantly enhanced HvAACT1 expression, especially in the root tips of Al-tolerant accessions. Furthermore, in a number of accessions with low levels of HvAACT1 expression, this MRL insertion was present but highly methylated. Geographical analysis showed that accessions with this MRL insertion are distributed mainly in European areas with acid soils. Two wild barley accessions were found to possess this MRL insertion, but with a high degree of methylation. These results indicate that the MRL insertion and its degree of DNA methylation influence HvAACT1 expression and that demethylation of this MRL insertion, which facilitates adaptation to acid soils, occurred following barley domestication. Moreover, our results indicate that barley accessions in East Asia and Europe have developed independent but equivalent strategies to withstand Al toxicity in acid soils.

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  • Engineering rice with lower grain arsenic

    Fenglin Deng, Naoki Yamaji, Jian Feng Ma, Sang-Kyu Lee, Jong-Seong Jeon, Enrico Martinoia, Youngsook Lee, Won-Yong Song

    PLANT BIOTECHNOLOGY JOURNAL   16 ( 10 )   1691 - 1699   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Arsenic (As) is a poisonous element that causes severe skin lesions and cancer in humans. Rice (Oryza sativa L.) is a major dietary source of As in humans who consume this cereal as a staple food. We hypothesized that increasing As vacuolar sequestration would inhibit its translocation into the grain and reduce the amount of As entering the food chain. We developed transgenic rice plants expressing two different vacuolar As sequestration genes, ScYCF1 and OsABCC1, under the control of the RCc3 promoter in the root cortical and internode phloem cells, along with a bacterial gamma-glutamylcysteine synthetase driven by the maize UBI promoter. The transgenic rice plants exhibited reduced root-to-shoot and internode-to-grain As translocation, resulting in a 70% reduction in As accumulation in the brown rice without jeopardizing agronomic traits. This technology could be used to reduce As intake, particularly in populations of South East Asia suffering from As toxicity and thereby improve human health.

    DOI: 10.1111/pbi.12905

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  • Two genes encoding a bacterial-type ATP-binding cassette transporter are implicated in aluminum tolerance in buckwheat. 査読

    Che J, Yamaji N, Yokosho K, Shen RF, Ma JF

    Plant & cell physiology   59 ( 12 )   2502 - 2511   2018年8月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/pcp/pcy171

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  • Efficient and flexible uptake system for mineral elements in plants 査読

    Jing Che, Naoki Yamaji, Jian Feng Ma

    New Phytologist   219 ( 2 )   513 - 517   2018年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Blackwell Publishing Ltd  

    (Table presented.). Summary: Mineral elements required for plant growth and development must first be taken up by the roots from soil. Plants have developed an efficient uptake system for the radial transport of mineral elements from soil to central stele through the allocation of various transporters at different root cells. These transporters are regulated at transcriptional, translational and/or post-translational level to cope with the fluctuation of mineral elements in soil. In this insight, we describe an efficient uptake system for mineral elements formed by influx and efflux transporters, regulatory mechanisms and polarity of these transporters, and sensing and signal pathways, in response to spatial and temporal changes of mineral elements in soil. An understanding of the mineral element uptake system in different plant species, and its regulatory network, will contribute to high and safe crop production under varying environments.

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  • A member of cation diffusion facilitator family, MTP11, is required for manganese tolerance and high fertility in rice 査読

    Yuta Tsunemitsu, Mayuko Genga, Tomoyuki Okada, Naoki Yamaji, Jian Feng Ma, Akira Miyazaki, Shin ichiro Kato, Kozo Iwasaki, Daisei Ueno

    Planta   248 ( 1 )   231 - 241   2018年7月

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    掲載種別:研究論文(学術雑誌)  

    © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Main conclusion: Rice MTP11 is the trans-Golgi-localized transporter that is involved in Mn tolerance with MTP8.1, and it is required for normal fertility. Rice (Oryza sativa L.) is one of the most manganese (Mn)-tolerant species, and it is able to accumulate high levels of this metal in the leaves without showing toxic symptoms. The metal tolerance protein 8.1 (MTP8.1), a member of the Mn-cation diffusion facilitator (CDF) family, has been shown to play a central role in high Mn tolerance by sequestering Mn into vacuoles. Recently, rice MTP11 was identified as an Mn transporter that is localized to Golgi-associated compartments, but its exact role in Mn tolerance in planta has not yet been understood. Here, we investigated the role of MTP11 in rice Mn tolerance using knockout lines. Old leaves presented higher levels of constitutively expressed MTP11 than other tissues and MTP11 expression was also found in reproductive organs. Fused MTP11:green fluorescent protein was co-localized to trans-Golgi markers and differentiated from other Golgi-associated markers. Knockout of MTP11 in wild-type rice did not affect tolerance and accumulation of Mn and other heavy metals, but knockout in the mtp8.1 mutant showed exacerbated Mn sensitivity at the vegetative growth stage. Knockout of MTP11 alone resulted in decreased grain yield and fertility at the reproductive stage. Thus, MTP11 is a trans-Golgi localized transporter for Mn, which plays a role in Mn tolerance through intracellular Mn compartmentalization. It is also required for maintaining high fertility in rice.

    DOI: 10.1007/s00425-018-2890-1

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  • The putative peptide gene FEP1 regulates iron deficiency response in Arabidopsis. 査読

    Hirayama T, Lei GJ, Yamaji N, Nakagawa N, Ma JF

    Plant & cell physiology   59 ( 9 )   1739 - 1752   2018年7月

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    掲載種別:研究論文(学術雑誌)  

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  • Effective reduction of cadmium accumulation in rice grain by expressing OsHMA3 under the control of the OsHMA2 promoter 査読

    Ji Feng Shao, Jixing Xia, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    Journal of Experimental Botany   69 ( 10 )   2743 - 2752   2018年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press  

    Reducing cadmium (Cd) accumulation in rice grain is an important issue for human health. The aim of this study was to manipulate both expression and tissue localization of OsHMA3, a tonoplast-localized Cd transporter, in the roots by expressing it under the control of the OsHMA2 promoter, which shows high expression in different organs including roots, nodes, and shoots. In two independent transgenic lines, the expression of OsHMA3 was significantly enhanced in all organs compared with non-transgenic rice. Furthermore, OsHMA3 protein was detected in the root pericycle cells and phloem region of both the diffuse vascular bundle and the enlarged vascular bundle of the nodes. At the vegetative stage, the Cd concentration in the shoots and xylem sap of the transgenic rice was significantly decreased, but that of the whole roots and root cell sap was increased. At the reproductive stage, the concentration of Cd, but not other essential metals, in the brown rice of transgenic lines was decreased to less than one-tenth that of the non-transgenic rice. These results indicate that expression of OsHMA3 under the control of the OsHMA2 promoter can effectively reduce Cd accumulation in rice grain through sequestering more Cd into the vacuoles of various tissues.

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  • Transport system of mineral elements in rice 査読

    Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    Rice Genomics, Genetics and Breeding   223 - 240   2018年3月

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    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Springer Singapore  

    Plant requires 14 mineral elements for their growth and development. These elements in the soil are taken up by the roots, translocated from the roots to the shoots, and distributed to different organs depending on their demands (Marschner P, Mineral nutrition of higher plants, 3rd edn. Academic, London, 2012). In addition to these essential elements, toxic elements such as Cd and As are also transported from the soils to aboveground parts. All these processes require various transporters (membrane proteins). During the last decades, a number of transporters for uptake, translocation, and distribution of mineral elements have been identified, especially in model plants such as Arabidopsis and rice
    however, most transporters remain to be identified. In this chapter, transporters identified so far in rice are described, and the regulation mechanisms of transporters in response to environmental changes are also discussed.

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  • OsNRT2.4 encodes a dual-affinity nitrate transporter and functions in nitrate-regulated root growth and nitrate distribution in rice 査読

    Jia Wei, Yi Zheng, Huimin Feng, Hongye Qu, Xiaorong Fan, Naoki Yamaji, Jian Feng Ma, Guohua Xu

    Journal of Experimental Botany   69 ( 5 )   1095 - 1107   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press  

    Plant NRT2 nitrate transporters commonly require a partner protein, NAR2, for transporting nitrate at low concentrations, but their role in plants is not well understood. In this study, we characterized the gene for one of these transporters in the rice genome, OsNRT2.4, in terms of its activity and roles in rice grown in environments with different N supply. In Xenopus oocytes, OsNRT2.4 alone without OsNAR2 co-expression facilitated nitrate uptake showing biphasic kinetics at a wide concentration range, with high- and low-affinity K M values of 0.15 and 4 mM, respectively. OsNRT2.4 did not have nitrate efflux or IAA influx activity. In rice roots, OsNRT2.4 was expressed mainly in the base of lateral root primordia. Knockout of OsNRT2.4 decreased lateral root number and length, and the total N uptake per plant at both 0.25 and 2.5 mM NO 3 - ' levels. In the shoots, OsNRT2.4 was expressed mainly in vascular tissues, and its knockout decreased the growth and NO 3 - ' -N distribution. Knockout of OsNRT2.4, however, did not affect rice growth and N uptake under conditions without N or with only NH 4 + supply. We conclude that OsNRT2.4 functions as a dual-affinity nitrate transporter and is required for nitrate-regulated root and shoot growth of rice.

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  • Preferential distribution of boron to developing tissues is mediated by the intrinsic protein OSNIP3 査読

    Ji Feng Shao, Naoki Yamaji, Xin Wei Liu, Kengo Yokosho, Ren Fang Shen, Jian Feng Ma

    Plant Physiology   176 ( 2 )   1739 - 1750   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society of Plant Biologists  

    Boron is especially required for the growth of meristem and reproductive organs, but the molecular mechanisms underlying the preferential distribution of B to these developing tissues are poorly understood. Here, we show evidence that a member of nodulin 26-like intrinsic protein (NIP), OsNIP3
    1, is involved in this preferential distribution in rice (Oryza sativa). OsNIP3
    1 was highly expressed in the nodes and its expression was up-regulated by B deficiency, but down-regulated by high B. OsNIP3
    1 was polarly localized at the xylem parenchyma cells of enlarged vascular bundles of nodes facing toward the xylem vessels. Furthermore, this protein was rapidly degraded within a few hours in response to high B. Knockout of this gene hardly affected the uptake and root-to-shoot translocation of B, but altered B distribution in different organs in the above-ground parts, decreased distribution of B to the new leaves, and increased distribution to the old leaves. These results indicate that OsNIP3
    1 located in the nodes is involved in the preferential distribution of B to the developing tissues by unloading B from the xylem in rice and that it is regulated at both transcriptional and protein level in response to external B level.

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  • Rice reduces Mn uptake in response to Mn stress 査読

    Yuta Tsunemitsu, Naoki Yamaji, Jian Feng Ma, Shin ichiro Kato, Kozo Iwasaki, Daisei Ueno

    Plant Signaling and Behavior   13 ( 1 )   2018年1月

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    掲載種別:研究論文(学術雑誌)  

    © 2018 Taylor & Francis Group, LLC. Rice (Oryza sativa L) is one of the most Mn-tolerant crops that can grow in submerged paddy fields, where the Mn concentration in soil solution is very high due to reduction. Although a large part of Mn is transferred from the roots to the shoot in rice, the roots are constantly exposed to high Mn concentrations in submerged paddies. Thus, mechanisms for preventing Mn overaccumulation in the cytoplasm of root cells are necessary. Recently, we showed that two cation diffusion facilitators, MTP8.1 and MTP8.2, play a crucial role in Mn tolerance in rice roots by sequestering Mn in vacuoles. Moreover, we observed that disruption of MTP8.1 and MTP8.2 resulted in reduced Mn accumulation under excess Mn. In the present study, we examined the effects of disruption of MTP8.1 and MTP8.2 on Mn uptake and determined that this phenotype is caused by a rapid and significant reduction of Mn uptake in response to excess Mn. Previously, we showed that Mn export from root cells through MTP9 was promoted by high Mn. Together, these findings suggest that optimal Mn concentration in rice roots is maintained by reduced uptake, vacuolar sequestration, and extrusion by cation diffusion facilitators.

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  • Functional characterization of an aluminum (Al)-inducible transcription factor, ART2, revealed a different pathway for Al tolerance in rice 査読

    Che Jing, Tsutsui Tomokazu, Yokosho Kengo, Yamaji Naoki, Ma Jian Feng

    New Phytologist   220 ( 1 )   209 - 218   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Both retrotransposon insertion and demethylation regulate aluminum tolerance of European barley for postdomestication expansion to acid soil.

    Fujii-Kashino, M, Yamaji, N, Yamane, M, Saisho, D, Sato, K, Ma, J. F

    Plant Physiology   2018年

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  • Two MATE Transporters with Different Subcellular Localization are Involved in Al Tolerance in Buckwheat 査読

    Gui Jie Lei, Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   58 ( 12 )   2179 - 2189   2017年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Buckwheat (Fagopyrum esculentum) shows high tolerance to aluminum (Al) toxicity, but the molecular mechanisms responsible for this high Al tolerance are still poorly understood. Here, we investigated the involvement of two MATE (multi-drug and toxic compound extrusion) genes in Al tolerance. Both FeMATE1 and FeMATE2 showed efflux transport activity for citrate, but not for oxalate when expressed in Xenopus oocytes. A transient assay with buckwheat leaf protoplasts using green fluorescent protein (GFP) fusion showed that FeMATE1 was mainly localized to the plasma membrane, whereas FeMATE2 was localized to the trans-Golgi and Golgi. The expression of FeMATE1 was induced by Al only in the roots, but that of FeMATE2 was up-regulated in both the roots and leaves. Furthermore, the expression of both genes only responded to Al toxicity, but not to other stresses including low pH, cadmium (Cd) and lanthanum (La). Heterologous expression of FeMATE1 or FeMATE2 in the Arabidopsis mutant atmate partially rescued its Al tolerance. Expression of FeMATE1 also partially recovered the Al-induced secretion of citrate in the transgenic lines, whereas expression of FeMATE2 did not complement the citrate secretion. Further physiological analysis showed that buckwheat roots also secreted citrate in addition to oxalate in response to Al in a dose-responsive manner. Taken together, our results indicate that FeMATE1 is involved in the Al-activated citrate secretion in the roots, while FeMATE2 is probably responsible for transporting citrate into the Golgi system for the internal detoxification of Al in the roots and leaves of buckwheat.

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  • Node-controlled allocation of mineral elements in Poaceae 査読

    Naoki Yamaji, Jian Feng Ma

    CURRENT OPINION IN PLANT BIOLOGY   39   18 - 24   2017年10月

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    記述言語:英語   出版者・発行元:CURRENT BIOLOGY LTD  

    Mineral elements taken up by the roots will be delivered to different organs and tissues depending on their requirements. In Poaceae, this selective distribution is mainly mediated in the nodes, which have highly developed and fully organized vascular systems. Inter-vascular transfer of mineral elements from enlarged vascular bundles to diffuse vascular bundles is required for their preferential distribution to developing tissues and reproductive organs. A number of transporters involved in this inter-vascular transfer processes have been identified mainly in rice. They are localized at the different cell layers and form an efficient machinery within the node. Furthermore, some these transporters show rapid response to the environmental changes of mineral elements at the protein level. In addition to the node-based transporters, distinct nodal structures including enlarged xylem area, folded plasma membrane of xylem transfer cells and presence of an apoplastic barrier are also required for the efficient inter-vascular transfer. Manipulation of node-based transporters will provide a novel breeding target to improve nutrient use efficiency, productivity, nutritional value and safety in cereal crops.

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  • The tonoplast-localized transporter MTP8.2 contributes to manganese detoxification in the shoots and roots of oryza sativa L. 査読

    Yuma Takemoto, Yuta Tsunemitsu, Miho Fujii-Kashino, Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma, Shin Ichiro Kato, Kozo Iwasaki, Daisei Ueno

    Plant and Cell Physiology   58 ( 9 )   1573 - 1582   2017年9月

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    掲載種別:研究論文(学術雑誌)  

    © The Author 2017. Manganese (Mn) cation diffusion facilitators (Mn-CDFs) play important roles in the Mn homeostasis of plants. In rice, the tonoplast-localized Mn-CDF metal tolerance protein 8.1 (MTP8.1) is involved in Mn detoxification in the shoots. This study functionally characterized the Mn-CDF MTP8.2 and determined its contribution to Mn tolerance. MTP8.2 was found to share 68% identity with MTP8.1 and was expressed in both the shoots and roots, but its transcription level was lower than that of MTP8.1. Transient expression of the MTP8.2:green fluorescent protein (GFP) fusion protein and immunoblotting studies indicated that MTP8.2 was also localized to the tonoplast. MTP8.2 expression in yeast conferred tolerance to Mn but not to Fe, Zn, Co, Ni or Cd. MTP8.2 knockdown caused further growth reduction of shoots and roots in the mtp8.1 mutant, which already exhibits stunted growth under conditions of excess Mn. In the presence of high Mn, the MTP8.2 knockdown lines of the mtp8.1 mutant showed lower root Mn concentrations, as well as lower root:total Mn ratios, than those of wild-type rice and the mtp8.1 mutant. These findings indicate that MTP8.2 mediates Mn tolerance along with MTP8.1 through the sequestration of Mn into the shoot and root vacuoles.

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  • Silicon reduces cadmium accumulation by suppressing expression of transporter genes involved in cadmium uptake and translocation in rice 査読

    Ji Feng Shao, Jing Che, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   68 ( 20 )   5641 - 5651   2017年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Silicon (Si) alleviates cadmium (Cd) toxicity and accumulation in a number of plant species, but the exact molecular mechanisms responsible for this effect are still poorly understood. Here, we investigated the effect of Si on Cd toxicity and accumulation in rice (Oryza sativa) by using two mutants (lsi1 and lsi2) defective in Si uptake and their wild types (WTs). Root elongation was decreased with increasing external Cd concentrations in both WTs and mutants, but Si did not show an alleviative effect on Cd toxicity in all lines. By contrast, the Cd concentration in both the shoots and roots was decreased by Si in the WTs, but not in the mutants. Furthermore, Si supply resulted in a decreased Cd concentration in the root cell sap and xylem sap in the WTs, but not in the mutants. Pre-treatment with Si also decreased Cd accumulation in the WTs, but not in the mutants. Silicon slightly decreased Cd accumulation in the cell wall of the roots. The expression level of OsNramp5 and OsHMA2 was down-regulated by Si in the WTs, but not in the mutants. These results indicate that the Si-decreased Cd accumulation was caused by down-regulating transporter genes involved in Cd uptake and translocation in rice.

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  • Functional characterization of two half-size ABC transporter genes in aluminium-accumulating buckwheat 査読

    Gui Jie Lei, Kengo Yokosho, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

    NEW PHYTOLOGIST   215 ( 3 )   1080 - 1089   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Buckwheat (Fagopyrum esculentum Moench) is able to detoxify high aluminium (Al) internally by sequestering it to the vacuoles in the leaves; however, the molecular mechanisms underlying this sequestration are unknown.
    We performed proteomic analysis with the leaf tonoplast-rich fraction and identified two half-size ABC transporters; FeASL1.1 and FeALS1.2. We investigated the gene expression patterns and subcellular localization. To demonstrate their physiological role, we expressed FeALS1.1 or FeALS1.2 in the Arabidopsis atals1 mutant under the control of AtALS1 promoter.
    FeALS1.1 expression was upregulated by Al in both the leaves and the roots, and its expression level in the roots was six times higher than its homologous gene (AtALS1) of Arabidopsis. FeALS1.2 expression, however, was not affected by Al but showed a 39 times higher expression level than AtALS1 in the leaves. When FeALS1.1 or FeALS1.2 was expressed in atals1, both of them recovered their Al tolerance through altering the subcellular localization of Al in root cells.
    Taken together, our results indicate that FeALS1.1 and FeALS1.2 are involved in the internal detoxification of Al in the roots and leaves, respectively, by sequestering Al into the vacuoles. Their high expression is probably required for high Al tolerance in buckwheat.

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  • OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice 査読

    Natsuko I. Kobayashi, Naoki Yamaji, Hiroki Yamamoto, Kaoru Okubo, Hiroki Ueno, Alex Costa, Keitaro Tanoi, Hideo Matsumura, Miho Fujii-Kashino, Tomoki Horiuchi, Mohammad Al Nayef, Sergey Shabala, Gynheung An, Jian Feng Ma, Tomoaki Horie

    PLANT JOURNAL   91 ( 4 )   657 - 670   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Salt tolerance quantitative trait loci analysis of rice has revealed that the SKC1 locus, which is involved in a higher K+/Na+ ratio in shoots, corresponds to the OsHKT1;5 gene encoding a Na+-selective transporter. However, physiological roles of OsHKT1;5 in rice exposed to salt stress remain elusive, and no OsHKT1;5 gene disruption mutants have been characterized to date. In this study, we dissected two independent TDNA insertional OsHKT1;5 mutants. Measurements of ion contents in tissues and 22 Na+ tracer imaging experiments showed that loss-of-function of OsHKT1;5 in salt-stressed rice roots triggers massive Na+ accumulation in shoots. Salt stress-induced increases in the OsHKT1;5 transcript were observed in roots and basal stems, including basal nodes. Immuno-staining using an anti-OsHKT1;5 peptide antibody indicated that OsHKT1;5 is localized in cells adjacent to the xylem in roots. Additionally, direct introduction of 22 Na+ tracer to leaf sheaths also demonstrated the involvement of OsHKT1;5 in xylem Na+ unloading in leaf sheaths. Furthermore, OsHKT1;5 was indicated to be present in the plasma membrane and found to localize also in the phloem of diffuse vascular bundles in basal nodes. Together with the characteristic 22 Na+ allocation in the blade of the developing immature leaf in the mutants, these results suggest a novel function of OsHKT1;5 in mediating Na+ exclusion in the phloem to prevent Na+ transfer to young leaf blades. Our findings further demonstrate that the function of OsHKT1;5 is crucial over growth stages of rice, including the protection of the next generation seeds as well as of vital leaf blades under salt stress.

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  • A Magnesium Transporter OsMGT1 Plays a Critical Role in Salt Tolerance in Rice(1[OPEN]) 査読

    Zhi Chang Chen, Naoki Yamaji, Tomoaki Horie, Jing Che, Jian Li, Gynheung An, Jian Feng Ma

    PLANT PHYSIOLOGY   174 ( 3 )   1837 - 1849   2017年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Salt stress is one of the major factors limiting rice (Oryza sativa) production globally. Although several transporters involved in salt tolerance have been identified in rice, the mechanisms regulating their transport activity are still poorly understood. Here, we show evidence that a rice Mg transporter OsMGT1 is required for salt tolerance probably by regulating transport activity of OsHKT1;5, a key transporter for the removal of Na+ from the xylem sap at the root mature zone. Knockout of OsMGT1 did not affect total Na uptake, but increased Na concentration in the shoots and xylem sap, resulting in a significant increase in salt sensitivity at low external Mg2+ concentration (20-200 mu M). However, such differences were abolished at a higher Mg2+ concentration (2 mM), although the total Na uptake was not altered. OsMGT1 was expressed in both the roots and shoots, but only that in the roots was moderately up-regulated by salt stress. Spatial expression analysis revealed that OsMGT1 was expressed in all root cells of the root tips but was highly expressed in the pericycle of root mature zone. OsMGT1 was also expressed in the phloem region of basal node, leaf blade, and sheath. When expressed in Xenopus laevis oocytes, the transport activity of OsHKT1;5 was enhanced by elevating external Mg2+ concentration. Furthermore, knockout of OsHKT1;5 in osmgt1 mutant background did not further increase its salt sensitivity. Taken together, our results suggest that Mg2+ transported by OsMGT1 in the root mature zone is required for enhancing OsHKT1;5 activity, thereby restricting Na accumulation to the shoots.

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  • A Model of Silicon Dynamics in Rice: An Analysis of the Investment Efficiency of Si Transporters 査読

    Gen Sakurai, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Keisuke Ono, Jian Feng Ma

    FRONTIERS IN PLANT SCIENCE   8   2017年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS MEDIA SA  

    Silicon is the second most abundant element in soils and is beneficial for plant growth. Although, the localizations and polarities of rice Si transporters have been elucidated, the mechanisms that control the expression of Si transporter genes and the functional reasons for controlling expression are not well-understood. We developed a new model that simulates the dynamics of Si in the whole plant in rice by considering Si transport in the roots, distribution at the nodes, and signaling substances controlling transporter gene expression. To investigate the functional reason for the diurnal variation of the expression level, we compared investment efficiencies (the amount of Si accumulated in the upper leaf divided by the total expression level of Si transporter genes) at different model settings. The model reproduced the gradual decrease and diurnal variation of the expression level of the transporter genes observed by previous experimental studies. The results of simulation experiments showed that a considerable reduction in the expression of Si transporter genes during the night increases investment efficiency. Our study suggests that rice has a system that maximizes the investment efficiency of Si uptake.

    DOI: 10.3389/10E-2017.01187

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  • The Key to Vn Homeostasis in Plants: Regulation of Mn Transporters 査読

    Ji Feng Shao, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    TRENDS IN PLANT SCIENCE   22 ( 3 )   215 - 224   2017年3月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE LONDON  

    Plants only require small amounts of manganese (Mn) for healthy growth, but Mn concentrations in soil solution vary from sub-micromolar to hundreds of micromolar across the growth period. Therefore, plants must deal with large Mn concentration fluctuations, but the molecular mechanisms underlying how plants cope with low and high Mn concentrations are poorly understood. In this Opinion we discuss the role of Mn transporters in the uptake, distribution, and detoxification of Mn in response to changes in Mn concentrations through their regulation at the transcriptional and protein levels, mainly focusing on rice, an Mn-tolerant and accumulating species. We also propose mechanisms involved in the hyperaccumulation of Mn and future prospects for studying this specific trait.

    DOI: 10.1016/j.tplants.2016.12.005

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  • ePro-ClearSee: a simple immunohistochemical method that does not require sectioning of plant samples 査読

    Kiyotaka Nagaki, Naoki Yamaji, Minoru Murata

    SCIENTIFIC REPORTS   7   2017年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Investigations into the epigenetic status of individual cells within tissues can produce both epigenetic data for different cell types and positional information of the cells. Thus, these investigations are important for understanding the intra-and inter-cellular control systems of developmental and environmental responses in plants. However, a simple method to detect epigenetic modifications of individual cells in plant tissues is not yet available because detection of the modifications requires immunohistochemistry using specific antibodies. In this study, we developed a simple immunohistochemical method that does not require sectioning to investigate epigenetic modifications. This method uses a clearing system to detect methylated histones, acetylated histones, methylated DNA and/or centromeric histone H3 variants. Analyses of four dicots and five monocots indicated that this method provides a universal technique to investigate epigenetic modifications in diverse plant species.

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  • Reducing phosphorus accumulation in rice grains with an impaired transporter in the node 査読

    Naoki Yamaji, Yuma Takemoto, Takaaki Miyaji, Namiki Mitani-Ueno, Kaoru T. Y. Oshida, Jian Feng Ma

    NATURE   541 ( 7635 )   92 - +   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Phosphorus is an important nutrient for crop productivity. More than 60% of the total phosphorus in cereal crops is finally allocated into the grains and is therefore removed at harvest. This removal accounts for 85% of the phosphorus fertilizers applied to the field each year(1,2). However, because humans and non-ruminants such as poultry, swine and fish cannot digest phytate, the major form of phosphorus in the grains, the excreted phosphorus causes eutrophication of waterways. A reduction in phosphorus accumulation in the grain would contribute to sustainable and environmentally friendly agriculture. Here we describe a rice transporter, SULTR-like phosphorus distribution transporter (SPDT), that controls the allocation of phosphorus to the grain. SPDT is expressed in the xylem region of both enlarged-and diffuse-vascular bundles of the nodes, and encodes a plasma-membrane-localized transporter for phosphorus. Knockout of this gene in rice (Oryza sativa) altered the distribution of phosphorus, with decreased phosphorus in the grains but increased levels in the leaves. Total phosphorus and phytate in the brown de-husked rice were 20-30% lower in the knockout lines, whereas yield, seed germination and seedling vigour were not affected. These results indicate that SPDT functions in the rice node as a switch to allocate phosphorus preferentially to the grains. This finding provides a potential strategy to reduce the removal of phosphorus from the field and lower the risk of eutrophication of waterways.

    DOI: 10.1038/nature20610

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  • Isolation and characterization of a rice line with high Cd accumulation for potential use in phytoremediation 査読

    Ji Feng Shao, Miho Fujii-Kashino, Naoki Yamaji, Shinichi Fukuoka, Ren Fang Shen, Jian Feng Ma

    PLANT AND SOIL   410 ( 1-2 )   357 - 368   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    To reduce cadmium (Cd) intake, remediation of Cd-contaminated soil and breeding crops with low Cd accumulation are important. This study aims to isolate rice mutants with altered accumulation of Cd.
    We used rice seeds mutated by N-methyl-N-nitrosourea for screening. The mutant was physiologically, genetically, and molecularly characterized. Cd accumulation was compared among five rice varieties cultivated in a Cd-contaminated soil.
    From 1000 lines screened, we isolated a line (TCM213) with high Cd accumulation. There was no difference in the root Cd uptake, but a higher root-to-shoot translocation of Cd was found in TCM213 compared with a common rice cultivar, T-65. The expression and sequence of OsNramp5 and OsHMA2 did not differ between TCM213 and T-65. However, several SNPs and deletion were found in the sequence of OsHMA3, although its expression and tissue localization were similar to those of T-65. Genetic analysis of an F-2 population derived from T-65 and TCM213 showed that the variation of OsHMA3 explained 72 % of variation in total Cd accumulation. TCM213 accumulated the largest Cd amount in the shoots among five Cd-accumulating varieties.
    High Cd accumulation in TCM213 results from loss of function of OsHMA3, and its high Cd accumulation has potential for efficient phytoremediation of Cd-contaminated soil.

    DOI: 10.1007/s11104-016-3014-y

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  • Reducing phosphorus accumulation in rice grains with an impaired transporter in the node (vol 541, pg 92, 2017) 査読

    Naoki Yamaji, Yuma Takemoto, Takaaki Miyaji, Namiki Mitani-Ueno, Kaoru T. Yoshida, Jian Feng Ma

    NATURE   541 ( 7635 )   136 - 136   2017年1月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    DOI: 10.1038/nature21404

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  • A model of silicon dynamics in rice 査読

    Gen Sakurai, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Keisuke Ono, Jian Feng Ma

    Frontiers in Plant Science   8   E31-E34   2017年

  • Analysis of epigenetic modifications in plant tissues by a novel clearing method 査読

    Kiyotaka Nagaki, Naoki Yamaji, Minoru Murata

    GENES & GENETIC SYSTEMS   91 ( 6 )   346 - 346   2016年12月

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    記述言語:英語   出版者・発行元:GENETICS SOC JAPAN  

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  • High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice 査読

    Namiki Mitani-Ueno, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   57 ( 12 )   2510 - 2518   2016年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Rice requires high silicon (Si) for its high and sustainable yield. The efficient uptake of Si in rice is mediated by two transporters OsLsi1 and OsLsi2, which function as influx and efflux transporters, respectively. Our previous studies showed that the mRNA expression levels of these transporter genes were down-regulated by Si. Herein we investigated the mechanism underlying regulation of OsLsi1 and OsLsi2 expression. There was a negative correlation between the expression level of OsLsi1 and OsLsi2 and shoot Si accumulation when the rice seedlings were exposed to different Si supply conditions. A split root experiment showed that the expression of both OsLsi1 and OsLsi2 was also downregulated in half the roots without direct Si exposure when the other half of the roots were exposed to Si. Analysis with transgenic rice carrying different lengths of OsLsi1 promoter regions fused with green fluorescent protein (GFP) as a reporter gene revealed that the region responsible for the Si response of OsLsi1 expression is present between -327 to -292 in the promoter. However, this region was not associated with the tissue and cellular localization of OsLsi1. In conclusion, the Si-induced down-regulation of Si transporter genes is controlled by shoot Si, not root Si, and the region between -327 and -292 in the OsLsi1 promoter is involved in this regulation of OsLsi1 expression in rice.

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  • Retrotransposon-Mediated Aluminum Tolerance through Enhanced Expression of the Citrate Transporter OsFRDL4 査読

    Kengo Yokosho, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

    PLANT PHYSIOLOGY   172 ( 4 )   2327 - 2336   2016年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    High aluminum (Al) tolerance of rice (Oryza sativa) is controlled by multiple tolerance genes, but the regulatory mechanisms underlying the differential expression of these genes are poorly understood. Here, we investigated the factors regulating the expression of OsFRDL4, a gene encoding a citrate efflux transporter involved in Al-induced citrate secretion from the roots. Analysis with chromosome segment substitution lines derived from cv Nipponbare (high OsFRDL4 expression) and cv Kasalath (low OsFRDL4 expression) revealed that the differential expression of OsFRDL4 is responsible for the quantitative trait locus for Al tolerance detected previously on chromosome 1. Comparison of the OsFRDL4 gene structure in cv Nipponbare and cv Kasalath showed that there was no difference in the position of the transcriptional start site, but a 1.2-kb insertion showing high similarity to the solo long terminal repeat of the retrotransposon was found in the promoter region of OsFRDL4 in cv Nipponbare. This insertion showed higher promoter activity and contained nine cis-acting elements for ALUMINUM RESISTANCE TRANSCRIPTION FACTOR1 (ART1). However, this insertion did not alter the spatial expression or cellular localization of OsFRDL4. Furthermore, this insertion was found in most japonica varieties but was largely absent from indica varieties or wild rice species. These results indicate that the 1.2-kb insertion in the OsFRDL4 promoter region in japonica subspecies is responsible for their higher expression level of OsFRDL4 due to the increased number of cis-acting elements of ART1. Our results also suggest that this insertion event happened at the initial stage of domestication of japonica subspecies.

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  • The HvNramp5 Transporter Mediates Uptake of Cadmium and Manganese, But Not Iron. 査読 国際誌

    Dezhi Wu, Naoki Yamaji, Miki Yamane, Miho Kashino-Fujii, Kazuhiro Sato, Jian Feng Ma

    Plant physiology   172 ( 3 )   1899 - 1910   2016年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS INC  

    The Natural Resistance Associated Macrophage Protein (Nramp) represents a transporter family for metal ions in all organisms. Here, we functionally characterized a member of Nramp family in barley (Hordeum vulgare), HvNramp5. This member showed different expression patterns, transport substrate specificity, and cellular localization from its close homolog in rice (Oryza sativa), OsNramp5, although HvNramp5 was also localized to the plasma membrane. HvNramp5 was mainly expressed in the roots and its expression was not affected by Cd and deficiency of Zn, Cu, and Mn, but slightly up-regulated by Fe deficiency. Spatial expression analysis showed that the expression of HvNramp5 was higher in the root tips than that in the basal root regions. Furthermore, analysis with laser microdissection revealed higher expression of HvNramp5 in the outer root cell layers. HvNramp5 showed transport activity for both Mn2+ and Cd2+, but not for Fe2+ when expressed in yeast. Immunostaining with a HvNramp5 antibody showed that this protein was localized in the root epidermal cells without polarity. Knockdown of HvNramp5 in barley resulted in a significant reduction in the seedling growth at low Mn supply, but this reduction was rescued at high Mn supply. The concentration of Mn and Cd, but not other metals including Cu, Zn, and Fe, was decreased in both the roots and shoots of knockdown lines compared with the wild-type barley. These results indicate that HvNramp5 is a transporter required for uptake of Mn and Cd, but not for Fe, and that barley has a distinct uptake system from rice.

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  • An Al-inducible expansin gene, OsEXPA10 is involved in root cell elongation of rice 査読

    Jing Che, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    PLANT JOURNAL   88 ( 1 )   132 - 142   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Expansins are cell wall loosening proteins, which are encoded by multigene families. However, the physiological role of most expansin genes is still poorly understood. Here, we functionally characterized an Al-inducible expansin gene, OsEXPA10, which is regulated by a C2H2-type zinc-finger transcription factor, ART1 in rice. A detailed expression analysis showed that OsEXPA10 was expressed in both the roots and shoots at a similar level, but only the expression in the roots was rapidly upregulated in response to Al. Furthermore, spatial expression analysis showed that the Al-induced expression was only found in the root tips (0-3 mm), but not in the mature root zones. The expression was neither induced by other metals including Cd and La nor by low pH. Immunostaining showed that OsEXPA10 was localized at all cells of the root tips. Knockout of OsEXPA10 resulted in a significant decrease in the cell elongation of the roots in the absence of Al. In the presence of Al, knockout of OsEXPA10 did not alter the Al sensitivity evaluated by relative root elongation, but the root cell wall of knockout lines accumulated less Al compared to those of the wild-type rice. Collectively, our results indicate that OsEXPA10 expressed in the root tips is required for the root cell elongation, but that the contribution of this gene to high Al tolerance in rice is small.

    DOI: 10.1111/tpj.13237

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  • OsFRDL1 expressed in nodes is required for distribution of iron to grains in rice 査読

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   67 ( 18 )   5485 - 5494   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    OsFRDL1 expressed in the upper nodes is required for the distribution of Fe to the panicles through solubilizing Fe deposited in the apoplastic part of nodes in rice.Iron (Fe) is essential for plant growth and development, but the molecular mechanisms underlying its distribution to different organs are poorly understood. We found that OsFRDL1 (FERRIC REDUCTASE DEFECTIVE LIKE 1), a plasma membrane-localized transporter for citrate, was highly expressed in the upper nodes of rice at the reproductive growth stage. OsFRDL1 was expressed in most cells of enlarged vascular bundles, diffuse vascular bundles, and the interjacent parenchyma cell bridges of uppermost node I, as well as vascular tissues of the leaf blade, leaf sheath, peduncle, rachis, husk, and stamen. Knockout of OsFRDL1 decreased pollen viability and grain fertility when grown in a paddy field. Iron was deposited in the parenchyma cell bridges, a few of the cell layers of the parenchyma tissues outside of the bundle sheath of enlarged vascular bundles in node I in both the wild-type rice and osfrdl1 mutant, but the mutant accumulated more Fe than the wild-type rice in this area. A stem-fed experiment with stable isotope Fe-57 showed that the distribution of Fe in the anther and panicle decreased in the knockout line, but that in the flag leaf it increased compared with the wild-type rice. Taken together, our results show that OsFRDL1 expressed in the upper nodes is required for the distribution of Fe in the panicles through solubilizing Fe deposited in the apoplastic part of nodes in rice.

    DOI: 10.1093/jxb/erw314

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  • A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain 査読

    Xin-Yuan Huang, Fenglin Deng, Naoki Yamaji, Shannon R. M. Pinson, Miho Fujii-Kashino, John Danku, Alex Douglas, Mary Lou Guerinot, David E. Salt, Jian Feng Ma

    NATURE COMMUNICATIONS   7   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Rice is a major source of calories and mineral nutrients for over half the world's human population. However, little is known in rice about the genetic basis of variation in accumulation of copper (Cu), an essential but potentially toxic nutrient. Here we identify OsHMA4 as the likely causal gene of a quantitative trait locus controlling Cu accumulation in rice grain. We provide evidence that OsHMA4 functions to sequester Cu into root vacuoles, limiting Cu accumulation in the grain. The difference in grain Cu accumulation is most likely attributed to a single amino acid substitution that leads to different OsHMA4 transport activity. The allele associated with low grain Cu was found in 67 of the 1,367 rice accessions investigated. Identification of natural allelic variation in OsHMA4 may facilitate the development of rice varieties with grain Cu concentrations tuned to both the concentration of Cu in the soil and dietary needs.

    DOI: 10.1038/ncomms12138

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  • Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice 査読

    Yosuke Toda, Yin Wang, Akira Takahashi, Yuya Kawai, Yasuomi Tada, Naoki Yamaji, Jian Feng Ma, Motoyuki Ashikari, Toshinori Kinoshita

    PLANT AND CELL PHYSIOLOGY   57 ( 6 )   1220 - 1230   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The stomatal apparatus consists of a pair of guard cells and regulates gas exchange between the leaf and atmosphere. In guard cells, blue light (BL) activates H+-ATPase in the plasma membrane through the phosphorylation of its penultimate threonine, mediating stomatal opening. Although this regulation is thought to be widely adopted among kidney-shaped guard cells in dicots, the molecular basis underlying that of dumbbell-shaped guard cells in monocots remains unclear. Here, we show that H+-ATPases are involved in the regulation of dumbbell-shaped guard cells. Stomatal opening of rice was promoted by the H+-ATPase activator fusicoccin and by BL, and the latter was suppressed by the H+-ATPase inhibitor vanadate. Using H+-ATPase antibodies, we showed the presence of phosphoregulation of the penultimate threonine in Oryza sativa H+-ATPases (OSAs) and localization of OSAs in the plasma membrane of guard cells. Interestingly, we identified one H+-ATPase isoform, OSA7, that is preferentially expressed among the OSA genes in guard cells, and found that loss of function of OSA7 resulted in partial insensitivity to BL. We conclude that H+-ATPase is involved in BL-induced stomatal opening of dumbbell-shaped guard cells in monocotyledon species.

    DOI: 10.1093/pcp/pcw070

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  • An Aluminum-Inducible IREG Gene is Required for Internal Detoxification of Aluminum in Buckwheat 査読

    Kengo Yokosho, Naoki Yamaji, Namiki Mitani-Ueno, Ren Fang Shen, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   57 ( 6 )   1169 - 1178   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Buckwheat (Fagopyrum esculentum Moench) is able to detoxify aluminum (Al) both externally and internally, but the molecular mechanisms underlying its high Al tolerance are not understood. We functionally characterized a gene (FeIREG1) belonging to IRON REGULATED/ferroportin in buckwheat, which showed high expression in our previous genome-wide transcriptome analysis. FeIREG1 was mainly expressed in the roots, and its expression was up-regulated by Al, but not by other metals and low pH. Furthermore, in contrast to AtIREG1 and AtIREG2 in Arabidopsis, the expression of FeIREG1 was not induced by Fe deficiency. Spatial expression analysis showed that the Al-induced expression of FeIREG1 was found in the root tips and higher expression was detected in the outer layers of this part. Immunostaining also showed that FeIREG1 was localized at the outer cell layers in the root tip. A FeIREG1-green fluorescent protein (GFP) fusion protein was localized to the tonoplast when transiently expressed in onion epidermal cells. Overexpression of FeIREG1 in Arabidopsis resulted in increased Al tolerance, but did not alter the tolerance to Cd, Co and Fe. The tolerance to Ni was slightly enhanced in the overexpression lines. Mineral analysis showed that the accumulation of total root Al and other essential mineral elements was hardly altered in the overexpression lines. Taken together, our results suggest that FeIREG1 localized at the tonoplast plays an important role in internal Al detoxification by sequestering Al into the root vacuoles in buckwheat.

    DOI: 10.1093/pcp/pcw065

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  • Transporters involved in mineral nutrient uptake in rice 査読

    Akimasa Sasaki, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   67 ( 12 )   3645 - 3653   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    One of the most important roles of plant roots is to take up essential mineral nutrients from the soil for use in plant growth and development. The uptake of mineral elements is mediated by various transporters belonging to different transporter families. Here we reviewed transporters for the uptake of macronutrients and micronutrients identified in rice, an important staple food for half of the world's population. Rice roots are characterized by having two Casparian strips on the exodermis and endodermis and by the formation of aerenchyma in the mature root zone. This distinct anatomical structure dictates that a pair of influx and efflux transporters at both the exodermis and endodermis is required for the radial transport of a mineral element from the soil solution to the stele. Some transporters showing polar localization at the distal and proximal sides of the exodermis and endodermis have been identified for silicon and manganese, forming an efficient uptake system. However, transporters for the uptake of most mineral elements remain to be identified.

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  • A Cation-Chloride Cotransporter Gene Is Required for Cell Elongation and Osmoregulation in Rice 査読

    Zhi Chang Chen, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

    PLANT PHYSIOLOGY   171 ( 1 )   494 - 507   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) is characterized by having fibrous root systems; however, the molecular mechanisms underlying the root development are not fully understood. Here, we isolated a rice mutant with short roots and found that the mutant had a decreased cell size of the roots and shoots compared with wild-type rice. Map-based cloning combined with whole-genome sequencing revealed that a single nucleotide mutation occurred in a gene, which encodes a putative cation-chloride cotransporter (OsCCC1). Introduction of OsCCC1 cDNA into the mutant rescued the mutant growth, indicating that growth defects of both the roots and shoots are caused by loss of function of OsCCC1. Physiological analysis showed that the mutant had a lower concentration of Cl- and K+ and lower osmolality in the root cell sap than the wild type at all KCl supply conditions tested; however, the mutant only showed a lower Na+ concentration at high external Na+. Expression of OsCCC1 in yeast increased accumulation of K+, Na+, and Cl-. The expression of OsCCC1 was found in both the roots and shoots, although higher expression was found in the root tips. Furthermore, the expression in the roots did not respond to different Na+, K+, and Cl- supply. OsCCC1 was expressed in all cells of the roots, leaf, and basal node. Immunoblot analysis revealed that OsCCC1 was mainly localized to the plasma membrane. These results suggest that OsCCC1 is involved in the cell elongation by regulating ion (Cl-, K+, and Na+) homeostasis to maintain cellular osmotic potential.

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  • Functional Analysis of a MATE Gene OsFRDL2 Revealed its Involvement in Al-Induced Secretion of Citrate, but a Lower Contribution to Al Tolerance in Rice 査読

    Kengo Yokosho, Naoki Yamaji, Miho Fujii-Kashino, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   57 ( 5 )   976 - 985   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The multidrug and toxic compound extrusion (MATE) transporters represent a large transporter family in plants, but the role of most genes in this family has not been examined. We functionally characterized a MATE family member, OsFRDL2, in rice (Oryza sativa). OsFRDL2 showed an efflux transport activity for citrate when it was expressed in both Xenopus oocytes and cultured tobacco cells. OsFRDL2 was mainly expressed in the roots and its expression was not induced by iron (Fe) deficiency, but it was rapidly up-regulated by aluminum (Al). Furthermore, the expression of OsFRDL2 was regulated by ART1, a C2H2-type zinc-finger transcription factor for Al tolerance. OsFRDL2 protein was localized at unidentified vesicles in the cytosol, but not co-localized with either mitochondria or peroxisomes when expressed in both onion epidermal cells and cultured tobacco cells. Knockout of OsFRDL2 decreased Al-induced secretion of citrate from the roots, but did not affect the internal citrate concentration. The Al-induced inhibition of root elongation was similar between the OsFRDL2 knockout line and its wild-type rice. Knockout of OsFRDL2 did not affect the translocation of Fe from the roots to the shoots. A double mutant between osfrdl2 and osfrdl4 or osfrdl1 did not further decrease the Al-induced citrate secretion and Fe translocation compared with the single mutant. Collectively, our results indicate that although OsFRDL2 is involved in the Al-induced secretion of citrate, its contribution to high Al tolerance is relatively small in rice.

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  • Silicon decreases both uptake and root-to-shoot translocation of manganese in rice 査読

    Jing Che, Naoki Yamaji, Ji Feng Shao, Jian Feng Ma, Ren Fang Shen

    JOURNAL OF EXPERIMENTAL BOTANY   67 ( 5 )   1535 - 1544   2016年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Silicon (Si) is known to alleviate manganese (Mn) toxicity in a number of plant species; however, the mechanisms responsible for this effect are poorly understood. Here, we investigated the interaction between Si and Mn in rice (Oryza sativa) by using a mutant defective in Si uptake. Silicon alleviated Mn toxicity in the wild-type (WT) rice, but not in the mutant exposed to high Mn. The Mn concentration in the shoots was decreased, but that in the roots was increased by Si in the WT. In contrast, the Mn concentration in the roots and shoots was unaffected by Si in the mutant. Furthermore, Si supply resulted in an increased Mn in the root cell sap, decreased Mn in the xylem sap in the WT, but these effects of Si were not observed in the mutant. A short-term labelling experiment with Mn-54 showed that the uptake of Mn was similar between plants with and without Si and between WT and the mutant. However, Si decreased root-to-shoot translocation of Mn in the WT, but not in the mutant. The expression of a Mn transporter gene for uptake, OsNramp5, was unaffected by a short exposure (&lt; 1 d) to Si, but down-regulated by relatively long-term exposure to Si in WT. In contrast, the expression of OsNramp5 was unaffected by Si in the mutant. These results indicated that Si-decreased Mn accumulation results from both Si-decreased root-to-shoot translocation of Mn, probably by the formation of Mn-Si complex in root cells, and uptake by down-regulating Mn transporter gene.

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  • OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress 査読

    Kei Suzuki, Naoki Yamaji, Alex Costa, Eiji Okuma, Natsuko I. Kobayashi, Tatsuhiko Kashiwagi, Maki Katsuhara, Cun Wang, Keitaro Tanoi, Yoshiyuki Murata, Julian I. Schroeder, Jian Feng Ma, Tomoaki Horie

    BMC PLANT BIOLOGY   16   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMED CENTRAL LTD  

    Background: Na+ exclusion from leaf blades is one of the key mechanisms for glycophytes to cope with salinity stress. Certain class I transporters of the high-affinity K+ transporter (HKT) family have been demonstrated to mediate leaf blade-Na+ exclusion upon salinity stress via Na+-selective transport. Multiple HKT1 transporters are known to function in rice (Oryza sativa). However, the ion transport function of OsHKT1;4 and its contribution to the Na+ exclusion mechanism in rice remain to be elucidated.
    Results: Here, we report results of the functional characterization of the OsHKT1; 4 transporter in rice. OsHKT1; 4 mediated robust Na+ transport in Saccharomyces cerevisiae and Xenopus laevis oocytes. Electrophysiological experiments demonstrated that OsHKT1; 4 shows strong Na+ selectivity among cations tested, including Li+, Na+, K+, Rb+, Cs+, and NH4+, in oocytes. A chimeric protein, EGFP-OsHKT1;4, was found to be functional in oocytes and targeted to the plasma membrane of rice protoplasts. The level of OsHKT1; 4 transcripts was prominent in leaf sheaths throughout the growth stages. Unexpectedly however, we demonstrate here accumulation of OsHKT1;4 transcripts in the stem including internode II and peduncle in the reproductive growth stage. Moreover, phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress. However, imposition of salinity stress on the RNAi plants in the reproductive growth stage caused significant Na+ overaccumulation in aerial organs, in particular, leaf blades and sheaths. In addition, Na-22(+) tracer experiments using peduncles of RNAi and WT plants suggested xylem Na+ unloading by OsHKT1;4.
    Conclusions: Taken together, our results indicate a newly recognized function of OsHKT1;4 in Na+ exclusion in stems together with leaf sheaths, thus excluding Na+ from leaf blades of a japonica rice cultivar in the reproductive growth stage, but the contribution is low when the plants are in the vegetative growth stage.

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  • 4-3-8 Two genes encoding bacterial-type ABC transporters are required for Al tolerance in buckwheat(4-3 植物の有害元素,2016年度佐賀大会)

    車 景, 横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   62   65 - 65   2016年

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    記述言語:英語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.62.0_65_2

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  • A polarly localized transporter for efficient manganese uptake in rice 査読

    Daisei Ueno, Akimasa Sasaki, Naoki Yamaji, Takaaki Miyaji, Yumi Fujii, Yuma Takemoto, Sawako Moriyama, Jing Che, Yoshinori Moriyama, Kozo Iwasaki, Jian Feng Ma

    NATURE PLANTS   1 ( 12 )   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Manganese is an essential metal for plant growth. A number of transporters involved in the uptake of manganese from soils, and its translocation to the shoot, have been identified in Arabidopsis and rice. However, the transporter responsible for the radial transport of manganese out of root exodermis and endodermis cells and into the root stele remains unknown. Here, we show that metal tolerance protein 9 (MTP9), a member of the cation diffusion facilitator family, is a critical player in this process in rice (Oryza sativa). We find that MTP9 is mainly expressed in roots, and that the resulting protein is localized to the plasma membrane of exo- and endodermis cells, at the proximal side of these cell layers (opposite the manganese uptake transporter Nramp5, which is found at the distal side). We demonstrate that MTP9 has manganese transport activity by expression in proteoliposomes and yeast, and show that knockout of MTP9 in rice reduces manganese uptake and its translocation to shoots. We conclude that at least in rice MTP9 is required for manganese translocation to the root stele, and thereby manganese uptake.

    DOI: 10.1038/NPLANTS.2015.170

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  • Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat 査読

    Kiyotaka Nagaki, Keisuke Tanaka, Naoki Yamaji, Hisato Kobayashi, Minoru Murata

    Frontiers in Plant Science   6 ( OCTOBER )   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Frontiers Research Foundation  

    The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequences and retrotransposons have been reported as centromeric DNA sequences. In this study on sunflowers, a cDNA-encoding centromere-specific histone H3 (CENH3) was isolated from a cDNA pool from a seedling, and an antibody was raised against a peptide synthesized from the deduced cDNA. The antibody specifically recognized the sunflower CENH3 (HaCENH3) and showed centromeric signals by immunostaining and immunohistochemical staining analysis. The antibody was also applied in chromatin immunoprecipitation (ChIP)-Seq to isolate centromeric DNA sequences and two different types of repetitive DNA sequences were identified. One was a long interspersed nuclear element (LINE)-like sequence, which showed centromere-specific signals on almost all chromosomes in sunflowers. This is the first report of a centromeric LINE sequence, suggesting possible centromere targeting ability. Another type of identified repetitive DNA was a tandem repeat sequence with a 187-bp unit that was found only on a pair of chromosomes. The HaCENH3 content of the tandem repeats was estimated to be much higher than that of the LINE, which implies centromere evolution from LINE-based centromeres to more stable tandem-repeat-based centromeres. In addition, the epigenetic status of the sunflower centromeres was investigated by immunohistochemical staining and ChIP, and it was found that centromeres were heterochromatic.

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  • A node-localized transporter OsZIP3 is responsible for the preferential distribution of Zn to developing tissues in rice 査読

    Akimasa Sasaki, Naoki Yamaji, Namiki Mitani-Ueno, Miho Kashino, Jian Feng Ma

    PLANT JOURNAL   84 ( 2 )   374 - 384   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Developing tissues such as meristem with low transpiration require high Zn levels for their active growth, but the molecular mechanisms underlying the preferential distribution to these tissues are poorly understood. We found that a member of the ZIP (ZRT, IRT-like protein), OsZIP3, showed high expression in the nodes of rice (Oryza sativa). Immunostaining revealed that OsZIP3 was localized at the xylem intervening parenchyma cells and xylem transfer cells of the enlarged vascular bundle in both basal and upper nodes. Neither OsZIP3 gene expression nor encoded protein was affected by either deficiency or toxic levels of Zn. Knockdown of OsZIP3 resulted in significantly reduced Zn levels in the shoot basal region containing the shoot meristem and elongating zone, but increased Zn levels in the transpiration flow. A short-term experiment with the Zn-67 stable isotope showed that more Zn was distributed to the lower leaves, but less to the shoot elongating zone and nodes in the knockdown lines compared with the wild-type rice at both the vegetative and reproductive growth stages. Taken together, OsZIP3 located in the node is responsible for unloading Zn from the xylem of enlarged vascular bundles, which is the first step for preferential distribution of Zn to the developing tissues in rice.
    Significance Statement Zinc homeostasis is achieved by regulating diverse zinc transporters. Here we show that the zinc transporter OsZIP3 mediates the first step leading to preferential distribution of zinc in developing tissues, by unloading zinc from the xylem of enlarged vascular bundles.

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  • Orchestration of three transporters and distinct vascular structures in node for intervascular transfer of silicon in rice 査読

    Naoki Yamaji, Gen Sakurai, Namiki Mitani-Ueno, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   112 ( 36 )   11401 - 11406   2015年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Requirement of mineral elements in different plant tissues is not often consistent with their transpiration rate; therefore, plants have developed systems for preferential distribution of mineral elements to the developing tissues with low transpiration. Here we took silicon (Si) as an example and revealed an efficient system for preferential distribution of Si in the node of rice (Oryza sativa). Rice is able to accumulate more than 10% Si of the dry weight in the husk, which is required for protecting the grains from water loss and pathogen infection. However, it has been unknown for a long time how this hyperaccumulation is achieved. We found that three transporters (Lsi2, Lsi3, and Lsi6) located at the node are involved in the intervascular transfer, which is required for the preferential distribution of Si. Lsi2 was polarly localized to the bundle sheath cell layer around the enlarged vascular bundles, which is next to the xylem transfer cell layer where Lsi6 is localized. Lsi3 was located in the parenchyma tissues between enlarged vascular bundles and diffuse vascular bundles. Similar to Lsi6, knockout of Lsi2 and Lsi3 also resulted in decreased distribution of Si to the panicles but increased Si to the flag leaf. Furthermore, we constructed a mathematical model for Si distribution and revealed that in addition to cooperation of three transporters, an apoplastic barrier localized at the bundle sheath cells and development of the enlarged vascular bundles in node are also required for the hyperaccumulation of Si in rice husk.

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  • A cooperative system of silicon transport in plants 査読

    Jian Feng Ma, Naoki Yamaji

    TRENDS IN PLANT SCIENCE   20 ( 7 )   435 - 442   2015年7月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE LONDON  

    The high accumulation of silicon (Si) protects plants from biotic and abiotic stresses. Two different types of Si transporter [Low Silicon 1 (Lsi1) and 2 (Lsi2)] involved in the uptake and distribution of Si have been identified. Lsi1, a Si permeable channel, belongs to the Nod26-like major intrinsic protein (NIP) III subgroup of the aquaporin membrane protein family with a distinct selectivity, whereas Lsi2, an efflux Si transporter, belongs to an uncharacterized anion transporter family. These transporters are localized to the plasma membrane, but, in different plant species, show different expression patterns and tissue or cellular localizations that are associated with different levels of Si accumulation. A recent mathematical modeling study revealed that cooperation of Lsi1 and Lsi2, which show a polarized localization, is required for the efficient transport of Si in rice.

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  • In Silico Simulation Modeling Reveals the Importance of the Casparian Strip for Efficient Silicon Uptake in Rice Roots 査読

    Gen Sakurai, Akiko Satake, Naoki Yamaji, Namiki Mitani-Ueno, Masayuki Yokozawa, Francois Gabriel Feugier, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   56 ( 4 )   631 - 639   2015年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Silicon (Si) uptake by the roots is mediated by two different transporters, Lsi1 (passive) and Lsi2 (active), in rice (Oryza sativa). Both transporters are polarly localized in the plasma membranes of exodermal (outer) and endodermal (inner) cells with Casparian strips. However, it is unknown how rice is able to take up large amounts of Si compared with other plants, and why rice Si transporters have a characteristic cellular localization pattern. To answer these questions, we simulated Si uptake by rice roots by developing a mathematical model based on a simple diffusion equation that also accounts for active transport by Lsi2. In this model, we calibrated the model parameters using in vivo experimental data on the Si concentrations in the xylem sap and a Monte Carlo method. In our simulation experiments, we compared the Si uptake between roots with various transporter and Casparian strip locations and estimated the Si transport efficiency of roots with different localization patterns and quantities of the Lsi transporters. We found that the Si uptake by roots that lacked Casparian strips was lower than that of normal roots. This suggests that the doublelayer structure of the Casparian strips is an important factor in the high Si uptake by rice. We also found that among various possible localization patterns, the most efficient one was that of the wild-type rice; this may explain the high Si uptake capacity of rice.

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  • The Rice CK2 Kinase Regulates Trafficking of Phosphate Transporters in Response to Phosphate Levels 査読

    Jieyu Chen, Yifeng Wang, Fei Wang, Jian Yang, Mingxing Gao, Changying Li, Yingyao Liu, Yu Liu, Naoki Yamaji, Jian Feng Ma, Javier Paz-Ares, Laurent Nussaume, Shuqun Zhang, Keke Yi, Zhongchang Wu, Ping Wu

    PLANT CELL   27 ( 3 )   711 - 723   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Phosphate transporters (PTs) mediate phosphorus uptake and are regulated at the transcriptional and posttranslational levels. In one key mechanism of posttranslational regulation, phosphorylation of PTs affects their trafficking from the endoplasmic reticulum (ER) to the plasma membrane. However, the kinase(s) mediating PT phosphorylation and the mechanism leading to ER retention of phosphorylated PTs remain unclear. In this study, we identified a rice (Oryza sativa) kinase subunit, CK2 beta 3, which interacts with PT2 and PT8 in a yeast two-hybrid screen. Also, the CK2 alpha 3/beta 3 holoenzyme phosphorylates PT8 under phosphate-sufficient conditions. This phosphorylation inhibited the interaction of PT8 with PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1, a key cofactor regulating the exit of PTs from the ER to the plasma membrane. Additionally, phosphorus starvation promoted CK2 beta 3 degradation, relieving the negative regulation of PT phosphorus-insufficient conditions. In accordance, transgenic expression of a nonphosphorylatable version of OsPT8 resulted in elevated levels of that protein at the plasma membrane and enhanced phosphorus accumulation and plant growth under various phosphorus regimes. Taken together, these results indicate that CK2 alpha 3/beta 3 negatively regulates PTs and phosphorus status regulates CK2 alpha 3/beta 3.

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  • AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis 査読

    Takaaki Miyaji, Takashi Kuromori, Yu Takeuchi, Naoki Yamaji, Kengo Yokosho, Atsushi Shimazawa, Eriko Sugimoto, Hiroshi Omote, Jian Feng Ma, Kazuo Shinozaki, Yoshinori Moriyama

    NATURE COMMUNICATIONS   6   5928   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4; 4 protein exhibit membrane potential- and Cl- dependent ascorbate uptake. The AtPHT4; 4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4; 4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4; 4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress.

    DOI: 10.1038/ncomms6928

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  • 植物栄養と数理モデルの接点ー数理モデルで植物栄養の仕組みを理解する

    馬建鋒, 小林高範, 山地直樹, 小林安文, 小山博之, 岩本明敏, 櫻井玄

    日本土壌肥料学会誌   86 ( 3 )   226 - 230   2015年

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    記述言語:日本語   出版者・発行元:日本土壌肥料學會  

    植物は生育に欠かせない14種類のミネラルを根により土壌から吸収し,根から地上部に輸送したあと,過不足無く各器官に分配する。近年これらのミネラルの吸収,転流,分配などに関与するトランスポーターが多く同定された。しかし,これらのトランスポーターは協調して働くと考えられるが,その統合的な理解はまだ不十分である。一方,植物は栄養不足やミネラル過剰などのミネラルストレスを常に受けているが,植物はこれらのストレスに対しても巧みな耐性機構を発達させてきた。近年ミネラルストレス耐性に関わる遺伝子が多く同定された。しかし,植物の細胞がどのようにこれらのミネラルストレスを感知し,またどのようにシグナルを下流に伝達するのかについてまだ研究が始まったばかりである。本資料では,若手研究者による最新の植物栄養学の成果を紹介するとともに,ミネラルの輸送を統合的に理解するために試みられているモデリングの研究を紹介し,植物栄養学の今後の発展の方向性を探りたい。

    DOI: 10.20710/dojo.86.3_232

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    その他リンク: http://agriknowledge.affrc.go.jp/RN/2010892619

  • Global Transcriptome Analysis of Al-Induced Genes in an Al-Accumulating Species, Common Buckwheat (Fagopyrum esculentum Moench) 査読

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   55 ( 12 )   2077 - 2091   2014年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Buckwheat (Fagopyrum esculentum Moench) is a species with high aluminum (Al) tolerance and accumulation. Although the physiological mechanisms for external and internal detoxification of Al have been well studied, the molecular mechanisms responsible are poorly understood. Here, we conducted a genome-wide transcriptome analysis of Al-responsive genes in the roots and leaves using RNA sequencing (RNA-Seq) technology. RNA-Seq generated reads ranging from 56 x 10(6) to 93 x 10(6). A total of 148,734 transcript contigs with an average length of 1,014 bp were assembled, generating 84,516 unigenes. Among them, 31,730 and 23,853 unigenes were annotated, respectively, in the NCBI plant database and TAIR database for Arabidopsis. Of the annotated genes, 4,067 genes in the roots and 2,663 genes in the leaves were up-regulated (&gt;2-fold) by Al exposure, while 2,456 genes in the roots and 2,426 genes in the leaves were down-regulated (&lt;2-fold) A few STOP1/ART1 (SENSITIVE TO PROTON RHIZOTOXICITY1/AL RESISTANCE TRANSCRIPTION FACTOR1)-regulated gene homologs including FeSTAR1, FeALS3 (ALUMINUM SENSITIVE3), FeALS1 (ALUMINUM SENSITIVE1), FeMATE1 and FeMATE2 (MULTIDRUG AND TOXIC COMPOUND EXTRUSION1 and 2) were also up-regulated in buckwheat, indicating some common Al tolerance mechanism across the species, although most STOP1/ART1-regulated gene homologs were not changed. Most genes involved in citric and oxalic acid biosynthesis were not significantly altered. Some transporter genes were highly expressed in the roots and leaves and responded to Al stress, implicating their role in Al tolerance and accumulation. Overall, our data provide a platform for further characterizing the functions of genes involved in Al tolerance and accumulation in buckwheat.

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  • A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain 査読

    Won-Yong Song, Tomohiro Yamaki, Naoki Yamaji, Donghwi Ko, Ki-Hong Jung, Miho Fujii-Kashino, Gynheung An, Enrico Martinoia, Youngsook Lee, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   111 ( 44 )   15699 - 15704   2014年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Arsenic (As) is a chronic poison that causes severe skin lesions and cancer. Rice (Oryza sativa L.) is a major dietary source of As; therefore, reducing As accumulation in the rice grain and thereby diminishing the amount of As that enters the food chain is of critical importance. Here, we report that a member of the Oryza sativa C-type ATP-binding cassette (ABC) transporter (OsABCC) family, OsABCC1, is involved in the detoxification and reduction of As in rice grains. We found that OsABCC1 was expressed in many organs, including the roots, leaves, nodes, peduncle, and rachis. Expression was not affected when plants were exposed to low levels of As but was up-regulated in response to high levels of As. In both the basal nodes and upper nodes, which are connected to the panicle, OsABCC1 was localized to the phloem region of vascular bundles. Furthermore, OsABCC1 was localized to the tonoplast and conferred phytochelatin-dependent As resistance in yeast. Knockout of OsABCC1 in rice resulted in decreased tolerance to As, but did not affect cadmium toxicity. At the reproductive growth stage, the As content was higher in the nodes and in other tissues of wild-type rice than in those of OsABCC1 knockout mutants, but was significantly lower in the grain. Taken together, our results indicate that OsABCC1 limits As transport to the grains by sequestering As in the vacuoles of the phloem companion cells of the nodes in rice.

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  • Overexpression of OsHMA3 enhances Cd tolerance and expression of Zn transporter genes in rice 査読

    Akimasa Sasaki, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   65 ( 20 )   6013 - 6021   2014年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    As a member of the heavy metal ATPase (HMA) family, OsHMA3 is a tonoplast-localized transporter for Cd in the roots of rice (Oryza sativa). Overexpression of OsHMA3 selectively reduces Cd accumulation in the grain. Further characterization in the present study revealed that overexpression of OsHMA3 also enhances the tolerance to toxic Cd. The growth of both the roots and shoots was similar in the absence of Cd between an OsHMA3-overexpressed line and vector control, but the Cd-inhibited growth was significantly alleviated in the OsHMA3-overexpressed line. The over-expressed line showed higher Cd concentration in the roots, but lower Cd concentration in the shoots compared with the wild-type rice and vector control line, indicating that overexpression of OsHMA3 enhanced vacuolar sequestration of Cd in the roots. The Zn concentration in the roots of the OsHMA3-overexpressed line was constantly higher than that of vector control, but the Zn concentration in the shoots was similar between the overexpressed line and vector control. Five transporter genes belonging to the ZIP family were constitutively up-regulated in the OsHMA3-overexpressed line. These results suggest that shoot Zn level was maintained by up-regulating these genes involved in the Zn uptake/translocation. Taken together, overexpression of OsHMA3 is an efficient way to reduce Cd accumulation in the grain and to enhance Cd tolerance in rice.

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  • The node, a hub for mineral nutrient distribution in graminaceous plants 査読

    Naoki Yamaji, Jian Feng Ma

    TRENDS IN PLANT SCIENCE   19 ( 9 )   556 - 563   2014年9月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE LONDON  

    Mineral elements, including both essential and toxic elements, are delivered to different tissues after they are taken up from the roots, but the mechanism (or mechanisms) underlying the distribution remains poorly understood. In graminaceous plants, this distribution occurs in nodes, which have a complex, well-organized vascular system. A transfer of mineral elements between different vascular bundles is required, especially for preferential distribution to developing tissues that have low transpiration but high nutrient requirements. This intervascular transfer is mediated by various transporters localized at different cells in the node. In this opinion article, we propose four modes of distribution for different mineral elements: xylem-switch, phloem-tropic, phloem-kickback, and minimum-shift, based on specific molecular transport processes identified in the nodes mainly of rice (Oryza sativa). We also discuss the prospects for future studies on mineral nutrient distribution in the nodes.

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  • Timing of the G1/S transition in tobacco pollen vegetative cells as a primary step towards androgenesis in vitro 査読

    Masaharu Kyo, Ai Nagano, Naoki Yamaji, Yuhki Hashimoto

    PLANT CELL REPORTS   33 ( 9 )   1595 - 1606   2014年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Mid-bicellular pollen vegetative cells in tobacco escape from G1 arrest and proceed to the G1/S transition towards androgenesis within 1 day under glutamine starvation conditions in vitro.
    In the Nicotiana tabacum pollen culture system, immature pollen grains at the mid-bicellular stage can mature in the presence of glutamine; however, if glutamine is absent, they deviate from their native cell fate in a few days. The glutamine-starved pollen grains cannot undergo maturation, even when supplied with glutamine later. Instead, they undergo cell division towards androgenesis slowly within 10 days in a medium containing appropriate nutrients. During the culture period, they ought to escape from G1 arrest to proceed into S phase as the primary step towards androgenesis. However, this event has not been experimentally confirmed. Here, we demonstrated that the pollen vegetative cells proceeded to the G1/S transition within approximately 15-36 h after the start of culture. These results were obtained by analyzing transgenic pollen possessing a fusion gene encoding nuclear-localizing GFP under the control of an E2F motif-containing promoter isolated from a gene encoding one of DNA replication licensing factors. Observations using a 5-ethynyl-2'-deoxyuridine DNA labeling and detection technique uncovered that the G1/S transition was soon followed by S phase. These hallmarks of vegetative cells undergoing dedifferentiation give us new insights into upstream events causing the G1/S transition and also provide a novel strategy to increase the frequency of the androgenic response in tobacco and other species, including recalcitrants.

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  • Differential expression of Nrat1 is responsible for Al-tolerance QTL on chromosome 2 in rice 査読

    Jixing Xia, Naoki Yamaji, Jing Che, Ren Fang Shen, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   65 ( 15 )   4297 - 4304   2014年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Although rice (Oryza sativa) is the most Al-tolerant species among small-grain cereal crops, there is wide genotypic variation in its tolerance to Al toxicity. A number of quantitative trait loci (QTLs) for Al tolerance have been detected, but the responsible genes have not been identified. By using chromosome segment substitution lines, this work found that Nrat1, a gene encoding an Al transporter, is responsible for a QTL previously detected on chromosome 2. Substitution of the chromosome segment containing Nrat1 from Koshihikari (Al-tolerant variety) by that from Kasalath (Al-sensitive variety) decreased Nrat1 expression and Al uptake and tolerance, but increased binding of Al to the cell wall. Nrat1 in Kasalath showed tissue localization similar to Koshihikari in the roots. Although Koshihikari and Kasalath differed in four amino acids in Nrat1 protein, Nrat1 from Kasalath also showed transport activity for Al. Analysis with site-directed mutagenesis revealed that these differences did not affect the Al-transport activity much. Furthermore, there was no correlation between Al tolerance and the open-reading-frame sequence differences in other rice varieties. On the other hand, there was good correlation between Nrat1 expression and Al tolerance; however, sequence comparison of the promoter region up to 2.1 kb did not give a clear difference between the Al-tolerant and -sensitive varieties. Taken together, these results indicate that differential expression of Nrat1 is responsible for the QTL for Al tolerance on chromosome 2, although the mechanism controlling Nrat1 expression remains to be examined.

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  • Physiological and molecular characterization of Si uptake in wild rice species 査読

    Namiki Mitani-Ueno, Hisao Ogai, Naoki Yamaji, Jian Feng Ma

    PHYSIOLOGIA PLANTARUM   151 ( 3 )   200 - 207   2014年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Cultivated rice (Oryza sativa) accumulates high concentration of silicon (Si), which is required for its high and sustainable production. High Si accumulation in cultivated rice is achieved by a high expression of both influx (Lsi1) and efflux (Lsi2) Si transporters in roots. Herein, we physiologically investigated Si uptake, isolated and functionally characterized Si transporters in six wild rice species with different genome types. Si uptake by the roots was lower in Oryza rufipogon, Oryza barthii (AA genome), Oryza australiensis (EE genome) and Oryza punctata (BB genome), but similar in Oryza glumaepatula and Oryza meridionalis (AA genome) compared with the cultivated rice (cv. Nipponbare). However, all wild rice species and the cultivated rice showed similar concentration of Si in the shoots when grown in a field. All species with AA genome showed the same amino acid sequence of both Lsi1 and Lsi2 as O. sativa, whereas species with EE and BB genome showed several nucleotide differences in both Lsi1 and Lsi2. However, proteins encoded by these genes also showed transport activity for Si in Xenopus oocyte. The mRNA expression of Lsi1 in all wild rice species was lower than that in the cultivated rice, whereas the expression of Lsi2 was lower in O. rufipogon and O. barthii but similar in other species. Similar cellular localization of Lsi1 and Lsi2 was observed in all wild rice as the cultivated rice. These results indicate that superior Si uptake, the important trait for rice growth, is basically conserved in wild and cultivated rice species.

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  • Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation 査読

    Matthew J. Milner, Namiki Mitani-Ueno, Naoki Yamaji, Kengo Yokosho, Eric Craft, Zhangjun Fei, Stephen Ebbs, M. Clemencia Zambrano, Jian Feng Ma, Leon V. Kochian

    PLANT JOURNAL   78 ( 3 )   398 - 410   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    The Zn/Cd hyperaccumulator, Noccaea caerulescens, has been studied extensively for its ability to accumulate high levels of Zn and Cd in its leaves. Previous studies have indicated that the Zn and Cd hyperaccumulation trait exhibited by this species involves different transport and tolerance mechanisms. It has also been well documented that certain ecotypes of N.caerulescens are much better Cd hyperaccumulators than others. However, there does not seem to be much ecotypic variation for Zn hyperaccumulation in N.caerulescens. In this study we employed a comparative transcriptomics approach to look at root and shoot gene expression in Ganges and Prayon plants in response to Cd stress to identify transporter genes that were more highly expressed in either the roots or shoots of the superior Cd accumulator, Ganges. Comparison of the transcriptomes from the two ecotypes of Noccaea caerulescens identified a number of genes that encoded metal transporters that were more highly expressed in the Ganges ecotype in response to Cd stress. Characterization of one of these transporters, NcNramp1, showed that it is involved in the influx of Cd across the endodermal plasma membrane and thus may play a key role in Cd flux into the stele and root-to-shoot Cd transport. NcNramp1 may be one of the main transporters involved in Cd hyperaccumulation in N.caerulescens and copy number variation appears to be the main reason for high NcNramp1 gene expression underlying the increased Cd accumulation in the Ganges ecotype.

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  • Normal root elongation requires arginine produced by argininosuccinate lyase in rice 査読

    Jixing Xia, Naoki Yamaji, Jing Che, Ren Fang Shen, Jian Feng Ma

    PLANT JOURNAL   78 ( 2 )   215 - 226   2014年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Plant roots play an important role in the uptake of water and nutrients, structural support and environmental sensing, but the molecular mechanisms involved in root development are poorly understood in rice (Oryza sativa), which is characterized by a dense fibrous root system. Here we report a rice mutant (red1 for root elongation defect 1) with short roots. Morphological and physiological analyses showed that the mutant had a shorter length from the quiescent center (QC) to the starting point of the elongation zone but a similar cell size and number of lateral and crown roots compared with the wild type. Furthermore, the mutant had similar radial structure and nutrient uptake patterns to the wild type. Map-based cloning revealed that the mutant phenotype was caused by a point mutation of a gene encoding an argininosuccinate lyase (ASL), catalyzing the last step of arginine biosynthesis. The OsASL1 gene has two distinct transcripts, OsASL1.1 and OsASL1.2, which result from different transcription start sites, but only OsASL1.1 was able to complement the mutant phenotype. OsASL1.1 was expressed in both the roots and shoots. The protein encoded by OsASL1.1 showed ASL activity in yeast. OsALS1.1 was localized to the plastid. The short root of the mutant was rescued by exogenous addition of arginine, but not by other amino acids. These results indicate that arginine produced by ASL is required for normal root elongation in rice.

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  • An appropriate concentration of arginine is required for normal root growth in rice 査読

    Jixing Xia, Naoki Yamaji, Jian Feng Ma

    Plant Signaling and Behavior   9 ( 4 )   e28717   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Landes Bioscience  

    Plant roots play an important role in uptake of water and nutrients, support of above-ground part and environmental sensing, but the molecular mechanisms underlying the root development are poorly understood in rice. We found that a gene (OsASL1) encoding argininosuccinate lyase is involved in normal root development of rice. OsASL1 cleaves argininosuccinate to arginine and fumarate reversibly, the last step in the arginine biosynthetic pathway. Here, we further characterized OsASL1 in terms of expression pattern, subcellular localization, and arginine effect on the root growth. A detailed expression analysis revealed that 2 transcripts of OsASL1, OsASL1.1 and OsASL1.2, showed different expression patterns
    OsASL1.1 was expressed in most organs throughout the whole growth period, whereas OsASL1.2 was mainly expressed in the roots. In contrast to plastid-localized OsASL1.1, OsASL1.2 was localized to the cytosol and nucleus. The short-root phenotype of the mutant was not rescued by exogenous addition of the sodium nitroprusside, a nitric oxide donor, but rescued by an appropriate concentration of Arg. Our results indicate that the subcellular localization was determined by the N terminus of OsASL1 and that appropriate concentration of Arg is required for normal root elongation in rice. © 2014 Landes Bioscience.

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  • An appropriate concentration of arginine is required for normal root growth in rice. 査読

    Xia J, Yamaji N, Ma JF

    Plant signaling & behavior   9 ( 3 )   e28717   2014年

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  • A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice 査読

    Fenglin Deng, Naoki Yamaji, Jixing Xia, Jian Feng Ma

    PLANT PHYSIOLOGY   163 ( 3 )   1353 - 1362   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Heavy metal-transporting P-type ATPase (HMA) has been implicated in the transport of heavy metals in plants. Here, we report the function and role of an uncharacterized member of HMA, OsHMA5 in rice (Oryza sativa). Knockout of OsHMA5 resulted in a decreased copper (Cu) concentration in the shoots but an increased Cu concentration in the roots at the vegetative stage. At the reproductive stage, the concentration of Cu in the brown rice was significantly lower in the mutants than in the wild-type rice; however, there was no difference in the concentrations of iron, manganese, and zinc between two independent mutants and the wild type. The Cu concentration of xylem sap was lower in the mutants than in the wild-type rice. OsHMA5 was mainly expressed in the roots at the vegetative stage but also in nodes, peduncle, rachis, and husk at the reproductive stage. The expression was up-regulated by excess Cu but not by the deficiency of Cu and other metals, including zinc, iron, and manganese, at the vegetative stage. Analysis of the transgenic rice carrying the OsHMA5 promoter fused with green fluorescent protein revealed that it was localized at the root pericycle cells and xylem region of diffuse vascular bundles in node I, vascular tissues of peduncle, rachis, and husk. Furthermore, immunostaining with an antibody against OsHMA5 revealed that it was localized to the plasma membrane. Expression of OsHMA5 in a Cu transport-defective mutant yeast (Saccharomyces cerevisiae) strain restored the growth. Taken together, OsHMA5 is involved in loading Cu to the xylem of the roots and other organs.

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  • Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family 査読

    Zonghui Chen, Yumi Fujii, Naoki Yamaji, Sakine Masuda, Yuma Takemoto, Takehiro Kamiya, Yusufujiang Yusuyin, Kozo Iwasaki, Shin Ichiro Kato, Masayoshi Maeshima, Jian Feng Ma, Daisei Ueno

    Journal of Experimental Botany   64 ( 14 )   4375 - 4387   2013年11月

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    掲載種別:研究論文(学術雑誌)  

    Manganese (Mn) is an essential micronutrient for plants, but is toxic when present in excess. The rice plant (Oryza sativa L.) accumulates high concentrations of Mn in the aerial parts; however, the molecular basis for Mn tolerance is poorly understood. In the present study, genes encoding Mn tolerance were screened for by expressing cDNAs of genes from rice shoots in Saccharomyces cerevisiae. A gene encoding a cation diffusion facilitator (CDF) family member, OsMTP8.1, was isolated, and its expression was found to enhance Mn accumulation and tolerance in S. cer-evisiae. In plants, OsMTP8.1 and its transcript were mainly detected in shoots. High or low supply of Mn moderately induced an increase or decrease in the accumulation of OsMTP8.1, respectively. OsMTP8.1 was detected in all cells of leaf blades through immunohistochemistry. OsMTP8.1 fused to green fluorescent protein was localized to the tonoplast. Disruption of OsMTP8.1 resulted in decreased chlorophyll levels, growth inhibition in the presence of high concentrations of Mn, and decreased accumulation of Mn in shoots and roots. However, there was no difference in the accumulation of other metals, including Zn, Cu, Fe, Mg, Ca, and K. These results suggest that OsMTP8.1 is an Mn-specific transporter that sequesters Mn into vacuoles in rice and is required for Mn tolerance in shoots. © The Author 2013.

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  • Adaptation to acidic soil is achieved by increased numbers of cis-acting elements regulating ALMT1 expression in Holcus lanatus 査読

    Zhi Chang Chen, Kengo Yokosho, Miho Kashino, Fang-Jie Zhao, Naoki Yamaji, Jian Feng Ma

    PLANT JOURNAL   76 ( 1 )   10 - 23   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Yorkshire fog (Holcus lanatus), which belongs to the Poaceae family and is a close relative of the agronomic crop oat (Avena sativa), is a widely adaptable grass species that is able to grow on highly acidic soils with high levels of Al, but the mechanism underlying the high Al tolerance is unknown. Here, we characterized two accessions of H.lanatus collected from an acid plot (soil pH 3.6, HL-A) and a neutral plot (pH 7.1, HL-N) in terms of Al tolerance, organic acid anion secretion and related gene expression. In response to Al (pH 4.5), the HL-A roots secreted approximately twice as much malate as the HL-N roots, but there was no difference in citrate secretion. Cloning of the gene HlALMT1 responsible for malate secretion showed that the encoded amino acid sequence did not differ between two accessions, but the expression level in the outer cell layers of the HL-A roots was twice as high as in the HL-N roots. This difference was not due to the genomic copy number, but was due to the number of cis-acting elements for an Al-responsive transcription factor (HlART1) in the promoter region of HlALMT1, as demonstrated by both a yeast one-hybrid assay and a transient assay in tobacco protoplasts. Furthermore, introduction of HlALMT1 driven by the HL-A promoter into rice resulted in significantly more Al-induced malate secretion than introduction of HlALMT1 driven by the HL-N promoter. These findings indicate that the adaptation of H.lanatus to acidic soils may be achieved by increasing number of cis-acting elements for ART1 in the promoter region of the HlALMT1 gene, enhancing the expression of HlALMT1 and the secretion of malate.

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  • A plasma membrane-localized small peptide is involved in rice aluminum tolerance 査読

    Jixing Xia, Naoki Yamaji, Jian Feng Ma

    PLANT JOURNAL   76 ( 2 )   345 - 355   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    A transcription factor for Al tolerance, ART1, regulates the expression of at least 30 genes in rice. Here we functionally characterized one of the downstream genes, OsCDT3, which encodes a predicted peptide of only 53 amino acid residues rich in cysteine. Knockdown of this gene resulted in decreased tolerance to Al, but did not affect the tolerance to Cd. The aluminum (Al) content in the root residues including cell wall and the plasma membrane of knockdown lines decreased, but the Al concentration in the root cell sap increased compared with those of the wild-type rice. OsCDT3 was mainly expressed in the roots and its expression was specifically induced by Al exposure, not by low pH and other metals. There was a small genotypic variation in OsCDT3 expression level, but no correlation between Al tolerance and the OsCDT3 variation was found among 17 rice cultivars. Analysis of pOsCDT3::GFP transgenic rice showed that OsCDT3 was expressed at all cells in the root tips. Transient expression of OsCDT3 fused with GFP at both N- and C-termini showed that OsCDT3 was anchored to the plasma membrane. Expression of OsCDT3 in yeast conferred tolerance to Al, but not to Cd. Furthermore, OsCDT3 did not show transport activity for Al in yeast, but was able to directly bind Al in vitro. Taken together, our results indicate that OsCDT3 anchoring to the plasma membrane may play a role in stopping entry of Al into the root cells by binding Al, therefore, contributing to high Al tolerance in rice.

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  • A node-based switch for preferential distribution of manganese in rice 査読

    Naoki Yamaji, Akimasa Sasaki, Ji Xing Xia, Kengo Yokosho, Jian Feng Ma

    NATURE COMMUNICATIONS   4   2442   2013年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Mineral nutrients, such as manganese, are required for the development of plants and their reproductive organs, but these can be toxic if accumulated at high concentrations. Therefore, plants must have a system for preferentially delivering an adequate amount of minerals to these organs for active growth and development, while preventing mineral overaccumulation in the face of changing environments. Here we show that a member of the Nramp transporter family, OsNramp3, functions as a switch in response to environmental Mn changes. OsNramp3 is constitutively expressed in the node, a junction of vasculatures connecting leaves, stems and panicles. At low Mn concentration, OsNramp3 preferentially transports Mn to young leaves and panicles. However, at high Mn concentration, the OsNramp3 protein is rapidly degraded within a few hours, resulting in the distribution of Mn to old tissues. Our results reveal the OsNramp3-mediated strategy of rice for adapting to a wide change of Mn in the environment.

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  • Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2 査読

    Naoki Yamaji, Jixing Xia, Namiki Mitani-Ueno, Kengo Yokosho, Jian Feng Ma

    PLANT PHYSIOLOGY   162 ( 2 )   927 - 939   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Developing tissues such as meristems and reproductive organs require high zinc, but the molecular mechanisms of how zinc taken up by the roots is preferentially delivered to these tissues with low transpiration are unknown. Here, we report that rice (Oryza sativa) heavy metal ATPase2 (OsHMA2), a member of P-type ATPases, is involved in preferential delivery of zinc to the developing tissues in rice. OsHMA2 was mainly expressed in the mature zone of the roots at the vegetative stage, but higher expression was also found in the nodes at the reproductive stage. The expression was unaffected by either zinc deficiency or zinc excess. OsHMA2 was localized at the pericycle of the roots and at the phloem of enlarged and diffuse vascular bundles in the nodes. Heterologous expression of OsHMA2 in yeast (Saccharomyces cerevisiae) showed influx transport activity for zinc as well as cadmium. Two independent Tos17 insertion lines showed decreased zinc concentration in the crown root tips, decreased concentration of zinc and cadmium in the upper nodes and reproductive organs compared with wild-type rice. Furthermore, a short-term labeling experiment with Zn-67 showed that the distribution of zinc to the panicle and uppermost node I was decreased, but that, to the lower nodes, was increased in the two mutants. Taken together, OsHMA2 in the nodes plays an important role in preferential distribution of zinc as well as cadmium through the phloem to the developing tissues.

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  • Divergence of expression pattern contributed to neofunctionalization of duplicated HD-Zip I transcription factor in barley 査読

    Shun Sakuma, Mohammad Pourkheirandish, Goetz Hensel, Jochen Kumlehn, Nils Stein, Akemi Tagiri, Naoki Yamaji, Jian Feng Ma, Hidenori Sassa, Takato Koba, Takao Komatsuda

    NEW PHYTOLOGIST   197 ( 3 )   939 - 948   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Barley (Hordeum vulgare) spikes are developmentally switched from two-rowed to six-rowed by a single recessive gene, six-rowed spike 1 (vrs1), which encodes a homeodomain-leucine zipper I class transcription factor. Vrs1 is a paralog of HvHox2 and both were generated by duplication of an ancestral gene. HvHox2 is conserved among cereals, whereas Vrs1 acquired its current function during the evolution of barley. It was unclear whether divergence of expression pattern or protein function accounted for the functionalization of Vrs1. Here, we conducted a comparative analysis of protein functions and gene expression between HvHox2 and Vrs1 to clarify the functionalization mechanism. We revealed that the transcriptional activation activity of HvHOX2 and VRS1 was conserved. In situ hybridization analysis showed that HvHox2 is localized in vascular bundles in developing spikes, whereas Vrs1 is expressed exclusively in the pistil, lemma, palea and lodicule of lateral spikelets. The transcript abundance of Vrs1 was &gt; 10-fold greater than that of HvHox2 during the pistil developmental stage, suggesting that the essential function of Vrs1 is to inhibit gynoecial development. We demonstrated the quantitative function of Vrs1 using RNAi transgenic plants and Vrs1 expression variants. Expression analysis of six-rowed spike mutants that are nonallelic to vrs1 showed that Vrs1 expression was up-regulated by Vrs4, whereas HvHox2 expression was not. These data demonstrate that the divergence of gene expression pattern contributed to the neofunctionalization of Vrs1.

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  • Divergence of expression pattern contributed to neofunctionalization of duplicated HD-Zip I transcription factor in barley 査読

    Shun Sakuma, Mohammad Pourkheirandish, Goetz Hensel, Jochen Kumlehn, Nils Stein, Akemi Tagiri, Naoki Yamaji, Jian Feng Ma, Hidenori Sassa, Takato Koba, Takao Komatsuda

    NEW PHYTOLOGIST   197 ( 3 )   939 - 948   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Barley (Hordeum vulgare) spikes are developmentally switched from two-rowed to six-rowed by a single recessive gene, six-rowed spike 1 (vrs1), which encodes a homeodomain-leucine zipper I class transcription factor. Vrs1 is a paralog of HvHox2 and both were generated by duplication of an ancestral gene. HvHox2 is conserved among cereals, whereas Vrs1 acquired its current function during the evolution of barley. It was unclear whether divergence of expression pattern or protein function accounted for the functionalization of Vrs1. Here, we conducted a comparative analysis of protein functions and gene expression between HvHox2 and Vrs1 to clarify the functionalization mechanism. We revealed that the transcriptional activation activity of HvHOX2 and VRS1 was conserved. In situ hybridization analysis showed that HvHox2 is localized in vascular bundles in developing spikes, whereas Vrs1 is expressed exclusively in the pistil, lemma, palea and lodicule of lateral spikelets. The transcript abundance of Vrs1 was &gt; 10-fold greater than that of HvHox2 during the pistil developmental stage, suggesting that the essential function of Vrs1 is to inhibit gynoecial development. We demonstrated the quantitative function of Vrs1 using RNAi transgenic plants and Vrs1 expression variants. Expression analysis of six-rowed spike mutants that are nonallelic to vrs1 showed that Vrs1 expression was up-regulated by Vrs4, whereas HvHox2 expression was not. These data demonstrate that the divergence of gene expression pattern contributed to the neofunctionalization of Vrs1.

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  • Chromosome Dynamics Visualized with an Anti-Centromeric Histone H3 Antibody in Allium 査読

    Kiyotaka Nagaki, Maki Yamamoto, Naoki Yamaji, Yasuhiko Mukai, Minoru Murata

    PLOS ONE   7 ( 12 )   2012年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Due to the ease with which chromosomes can be observed, the Allium species, and onion in particular, have been familiar materials employed in cytogenetic experiments in biology. In this study, centromeric histone H3 (CENH3)-coding cDNAs were identified in four Allium species (onion, welsh onion, garlic and garlic chives) and cloned. Anti-CENH3 antibody was then raised against a deduced amino acid sequence of CENH3 of welsh onion. The antibody recognized all CENH3 orthologs of the Allium species tested. Immunostaining with the antibody enabled clear visualization of chromosome behavior during mitosis in the species. Furthermore, three-dimensional (3D) observation of mitotic cell division was achieved by subjecting root sections to immunohistochemical techniques. The 3D dynamics of the cells and position of cell-cycle marker proteins (CENH3 and alpha-tubulin) were clearly revealed by immunohistochemical staining with the antibodies. The immunohistochemical analysis made it possible to establish an overview of the location of dividing cells in the root tissues. This breakthrough in technique, in addition to the two centromeric DNA sequences isolated from welsh onion by chromatin immuno-precipitation using the antibody, should lead to a better understanding of plant cell division. A phylogenetic analysis of Allium CENH3s together with the previously reported plant CENH3s showed two separate clades for monocot species tested. One clade was made from CENH3s of the Allium species with those of Poaceae species, and the other from CENH3s of a holocentric species (Luzula nivea). These data may imply functional differences of CENH3s between holocentric and monocentric species. Centromeric localization of DNA sequences isolated from welsh onion by chromatin immunoprecipitation (ChIP) using the antibody was confirmed by fluorescence in situ hybridization and ChIP-quantitative PCR.

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  • Chromosome Dynamics Visualized with an Anti-Centromeric Histone H3 Antibody in Allium 査読

    Kiyotaka Nagaki, Maki Yamamoto, Naoki Yamaji, Yasuhiko Mukai, Minoru Murata

    PLOS ONE   7 ( 12 )   2012年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Due to the ease with which chromosomes can be observed, the Allium species, and onion in particular, have been familiar materials employed in cytogenetic experiments in biology. In this study, centromeric histone H3 (CENH3)-coding cDNAs were identified in four Allium species (onion, welsh onion, garlic and garlic chives) and cloned. Anti-CENH3 antibody was then raised against a deduced amino acid sequence of CENH3 of welsh onion. The antibody recognized all CENH3 orthologs of the Allium species tested. Immunostaining with the antibody enabled clear visualization of chromosome behavior during mitosis in the species. Furthermore, three-dimensional (3D) observation of mitotic cell division was achieved by subjecting root sections to immunohistochemical techniques. The 3D dynamics of the cells and position of cell-cycle marker proteins (CENH3 and alpha-tubulin) were clearly revealed by immunohistochemical staining with the antibodies. The immunohistochemical analysis made it possible to establish an overview of the location of dividing cells in the root tissues. This breakthrough in technique, in addition to the two centromeric DNA sequences isolated from welsh onion by chromatin immuno-precipitation using the antibody, should lead to a better understanding of plant cell division. A phylogenetic analysis of Allium CENH3s together with the previously reported plant CENH3s showed two separate clades for monocot species tested. One clade was made from CENH3s of the Allium species with those of Poaceae species, and the other from CENH3s of a holocentric species (Luzula nivea). These data may imply functional differences of CENH3s between holocentric and monocentric species. Centromeric localization of DNA sequences isolated from welsh onion by chromatin immunoprecipitation (ChIP) using the antibody was confirmed by fluorescence in situ hybridization and ChIP-quantitative PCR.

    DOI: 10.1371/journal.pone.0051315

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  • Functional Characterization of a Silicon Transporter Gene Implicated in Silicon Distribution in Barley 査読

    Naoki Yamaji, Yukako Chiba, Namiki Mitani-Ueno, Jian Feng Ma

    PLANT PHYSIOLOGY   160 ( 3 )   1491 - 1497   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Silicon (Si) is a beneficial element for plant growth. In barley (Hordeum vulgare), Si uptake by the roots is mainly mediated by a Si channel, Low Silicon1 (HvLsi1), and an efflux transporter, HvLsi2. However, transporters involved in the distribution of Si in the shoots have not been identified. Here, we report the functional characterization of a homolog of HvLsi1, HvLsi6. HvLsi6 showed permeability for Si and localized to the plasma membrane. At the vegetative growth stage, HvLsi6 was expressed in both the roots and shoots. The expression level was unaffected by Si supply. In the roots, HvLsi6 was localized in epidermis and cortex cells of the tips, while in the leaf blades and sheaths, HvLsi6 was only localized at parenchyma cells of vascular bundles. At the reproductive growth stage, high expression of HvLsi6 was also found in the nodes. HvLsi6 in node I was polarly located at the transfer cells surrounding the enlarged vascular bundles toward the numerous xylem vessels. These results suggest that HvLsi6 is involved in Si uptake in the root tips, xylem unloading of Si in leaf blade and sheath, and intervascular transfer of Si in the nodes. Furthermore, HvLsi2 was found to be localized at the parenchyma cell layer adjacent to the transfer cells with opposite polarity of HvLsi6, suggesting that the coupling of HvLsi6 and HvLsi2 is involved in the intervascular transfer of Si at the nodes. Si translocated via the enlarged vascular bundles is unloaded to the transfer cells by HvLsi6, followed by HvLsi2 to reload Si to the diffuse vascular bundles, which are connected to the upper part of the plant, especially the panicles, the ultimate Si sink.

    DOI: 10.1104/pp.112.204578

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  • Functional Characterization of a Silicon Transporter Gene Implicated in Silicon Distribution in Barley 査読

    Naoki Yamaji, Yukako Chiba, Namiki Mitani-Ueno, Jian Feng Ma

    PLANT PHYSIOLOGY   160 ( 3 )   1491 - 1497   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Silicon (Si) is a beneficial element for plant growth. In barley (Hordeum vulgare), Si uptake by the roots is mainly mediated by a Si channel, Low Silicon1 (HvLsi1), and an efflux transporter, HvLsi2. However, transporters involved in the distribution of Si in the shoots have not been identified. Here, we report the functional characterization of a homolog of HvLsi1, HvLsi6. HvLsi6 showed permeability for Si and localized to the plasma membrane. At the vegetative growth stage, HvLsi6 was expressed in both the roots and shoots. The expression level was unaffected by Si supply. In the roots, HvLsi6 was localized in epidermis and cortex cells of the tips, while in the leaf blades and sheaths, HvLsi6 was only localized at parenchyma cells of vascular bundles. At the reproductive growth stage, high expression of HvLsi6 was also found in the nodes. HvLsi6 in node I was polarly located at the transfer cells surrounding the enlarged vascular bundles toward the numerous xylem vessels. These results suggest that HvLsi6 is involved in Si uptake in the root tips, xylem unloading of Si in leaf blade and sheath, and intervascular transfer of Si in the nodes. Furthermore, HvLsi2 was found to be localized at the parenchyma cell layer adjacent to the transfer cells with opposite polarity of HvLsi6, suggesting that the coupling of HvLsi6 and HvLsi2 is involved in the intervascular transfer of Si at the nodes. Si translocated via the enlarged vascular bundles is unloaded to the transfer cells by HvLsi6, followed by HvLsi2 to reload Si to the diffuse vascular bundles, which are connected to the upper part of the plant, especially the panicles, the ultimate Si sink.

    DOI: 10.1104/pp.112.204578

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  • Comparative Genome-Wide Transcriptional Analysis of Al-Responsive Genes Reveals Novel Al Tolerance Mechanisms in Rice 査読

    Tomokazu Tsutsui, Naoki Yamaji, Chao Feng Huang, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

    PLOS ONE   7 ( 10 )   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Rice (Oryza sativa) is the most aluminum (Al)-tolerant crop among small-grain cereals, but the mechanism underlying its high Al resistance is still not well understood. To understand the mechanisms underlying high Al-tolerance, we performed a comparative genome-wide transcriptional analysis by comparing expression profiling between the Al-tolerance cultivar (Koshihikari) and an Al-sensitive mutant star1 (SENSITIVE TO AL RHIZOTOXICITY 1) in both the root tips and the basal roots. Exposure to 20 mu M AlCl3 for 6 h resulted in up-regulation (higher than 3-fold) of 213 and 2015 genes including 185 common genes in the root tips of wild-type and the mutant, respectively. On the other hand, in the basal root, genes up-regulated by Al were 126 and 2419 including 76 common genes in the wild-type and the mutant, respectively. These results indicate that Al-response genes are not only restricted to the root tips, but also in the basal root region. Analysis with genes up-or down-regulated only in the wild-type reveals that there are other mechanisms for Al-tolerance except for a known transcription factor ART1-regulated one in rice. These mechanisms are related to nitrogen assimilation, secondary metabolite synthesis, cell-wall synthesis and ethylene synthesis. Although the exact roles of these putative tolerance genes remain to be examined, our data provide a platform for further work on Al-tolerance in rice.

    DOI: 10.1371/journal.pone.0048197

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  • Comparative Genome-Wide Transcriptional Analysis of Al-Responsive Genes Reveals Novel Al Tolerance Mechanisms in Rice 査読

    Tomokazu Tsutsui, Naoki Yamaji, Chao Feng Huang, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

    PLOS ONE   7 ( 10 )   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Rice (Oryza sativa) is the most aluminum (Al)-tolerant crop among small-grain cereals, but the mechanism underlying its high Al resistance is still not well understood. To understand the mechanisms underlying high Al-tolerance, we performed a comparative genome-wide transcriptional analysis by comparing expression profiling between the Al-tolerance cultivar (Koshihikari) and an Al-sensitive mutant star1 (SENSITIVE TO AL RHIZOTOXICITY 1) in both the root tips and the basal roots. Exposure to 20 mu M AlCl3 for 6 h resulted in up-regulation (higher than 3-fold) of 213 and 2015 genes including 185 common genes in the root tips of wild-type and the mutant, respectively. On the other hand, in the basal root, genes up-regulated by Al were 126 and 2419 including 76 common genes in the wild-type and the mutant, respectively. These results indicate that Al-response genes are not only restricted to the root tips, but also in the basal root region. Analysis with genes up-or down-regulated only in the wild-type reveals that there are other mechanisms for Al-tolerance except for a known transcription factor ART1-regulated one in rice. These mechanisms are related to nitrogen assimilation, secondary metabolite synthesis, cell-wall synthesis and ethylene synthesis. Although the exact roles of these putative tolerance genes remain to be examined, our data provide a platform for further work on Al-tolerance in rice.

    DOI: 10.1371/journal.pone.0048197

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  • Comparative Genome-Wide Transcriptional Analysis of Al-Responsive Genes Reveals Novel Al Tolerance Mechanisms in Rice 査読

    Tomokazu Tsutsui, Naoki Yamaji, Chao Feng Huang, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

    PLOS ONE   7 ( 10 )   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Rice (Oryza sativa) is the most aluminum (Al)-tolerant crop among small-grain cereals, but the mechanism underlying its high Al resistance is still not well understood. To understand the mechanisms underlying high Al-tolerance, we performed a comparative genome-wide transcriptional analysis by comparing expression profiling between the Al-tolerance cultivar (Koshihikari) and an Al-sensitive mutant star1 (SENSITIVE TO AL RHIZOTOXICITY 1) in both the root tips and the basal roots. Exposure to 20 mu M AlCl3 for 6 h resulted in up-regulation (higher than 3-fold) of 213 and 2015 genes including 185 common genes in the root tips of wild-type and the mutant, respectively. On the other hand, in the basal root, genes up-regulated by Al were 126 and 2419 including 76 common genes in the wild-type and the mutant, respectively. These results indicate that Al-response genes are not only restricted to the root tips, but also in the basal root region. Analysis with genes up-or down-regulated only in the wild-type reveals that there are other mechanisms for Al-tolerance except for a known transcription factor ART1-regulated one in rice. These mechanisms are related to nitrogen assimilation, secondary metabolite synthesis, cell-wall synthesis and ethylene synthesis. Although the exact roles of these putative tolerance genes remain to be examined, our data provide a platform for further work on Al-tolerance in rice.

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  • YSL16 Is a Phloem-Localized Transporter of the Copper-Nicotianamine Complex That Is Responsible for Copper Distribution in Rice 査読

    Luqing Zheng, Naoki Yamaji, Kengo Yokosho, Jian Feng Ma

    PLANT CELL   24 ( 9 )   3767 - 3782   2012年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Cu is an essential element for plant growth, but the molecular mechanisms of its distribution and redistribution within the plants are unknown. Here, we report that Yellow stripe-like16 (YSL16) is involved in Cu distribution and redistribution in rice (Oryza sativa). Rice YSL16 was expressed in the roots, leaves, and unelongated nodes at the vegetative growth stage and highly expressed in the upper nodes at the reproductive stage. YSL16 was expressed at the phloem of nodes and vascular tissues of leaves. Knockout of this gene resulted in a higher Cu concentration in the older leaves but a lower concentration in the younger leaves at the vegetative stage. At the reproductive stage, a higher Cu concentration was found in the flag leaf and husk, but less Cu was present in the brown rice, resulting in a significant reduction in fertility in the knockout line. Isotope labeling experiments with Cu-65 showed that the mutant lost the ability to transport Cu-nicotianamine from older to younger leaves and from the flag leaf to the panicle. Rice YSL16 transported the Cu-nicotianamine complex in yeast. Taken together, our results indicate that Os-YSL16 is a Cu-nicotianamine transporter that is required for delivering Cu to the developing young tissues and seeds through phloem transport.

    DOI: 10.1105/tpc.112.103820

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  • YSL16 Is a Phloem-Localized Transporter of the Copper-Nicotianamine Complex That Is Responsible for Copper Distribution in Rice 査読

    Luqing Zheng, Naoki Yamaji, Kengo Yokosho, Jian Feng Ma

    PLANT CELL   24 ( 9 )   3767 - 3782   2012年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Cu is an essential element for plant growth, but the molecular mechanisms of its distribution and redistribution within the plants are unknown. Here, we report that Yellow stripe-like16 (YSL16) is involved in Cu distribution and redistribution in rice (Oryza sativa). Rice YSL16 was expressed in the roots, leaves, and unelongated nodes at the vegetative growth stage and highly expressed in the upper nodes at the reproductive stage. YSL16 was expressed at the phloem of nodes and vascular tissues of leaves. Knockout of this gene resulted in a higher Cu concentration in the older leaves but a lower concentration in the younger leaves at the vegetative stage. At the reproductive stage, a higher Cu concentration was found in the flag leaf and husk, but less Cu was present in the brown rice, resulting in a significant reduction in fertility in the knockout line. Isotope labeling experiments with Cu-65 showed that the mutant lost the ability to transport Cu-nicotianamine from older to younger leaves and from the flag leaf to the panicle. Rice YSL16 transported the Cu-nicotianamine complex in yeast. Taken together, our results indicate that Os-YSL16 is a Cu-nicotianamine transporter that is required for delivering Cu to the developing young tissues and seeds through phloem transport.

    DOI: 10.1105/tpc.112.103820

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  • Up-Regulation of a Magnesium Transporter Gene OsMGT1 Is Required for Conferring Aluminum Tolerance in Rice 査読

    Zhi Chang Chen, Naoki Yamaji, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

    PLANT PHYSIOLOGY   159 ( 4 )   1624 - 1633   2012年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Magnesium (Mg)-mediated alleviation of aluminum (Al) toxicity has been observed in a number of plant species, but the mechanisms underlying the alleviation are still poorly understood. When a putative rice (Oryza sativa) Mg transporter gene, Oryza sativa MAGNESIUM TRANSPORTER1 (OsMGT1), was knocked out, the tolerance to Al, but not to cadmium and lanthanum, was decreased. However, this inhibition could be rescued by addition of 10 mu M Mg, but not by the same concentration of barium or strontium. OsMGT1 was expressed in both the roots and shoots in the absence of Al, but the expression only in the roots was rapidly up-regulated by Al. Furthermore, the expression did not respond to low pH and other metals including cadmium and lanthanum, and was regulated by an Al-responsive transcription factor, AL RESISTANCE TRANSCRIPTION FACTOR1. An investigation of subcellular localization showed that OsMGT1 was localized to the plasma membrane. A short-term (30 min) uptake experiment with stable isotope Mg-25 showed that knockout of OsMGT1 resulted in decreased Mg uptake, but that the uptake in the wild type was enhanced by Al. Mg concentration in the cell sap of the root tips was also increased in the wild-type rice, but not in the knockout lines in the presence of Al. A microarray analysis showed that transcripts of genes related to stress were more up- and down-regulated in the knockout lines. Taken together, our results indicate that OsMGT1 is a transporter for Mg uptake in the roots and that up-regulation of this gene is required for conferring Al tolerance in rice by increasing Mg concentration in the cell.

    DOI: 10.1104/pp.112.199778

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  • Up-Regulation of a Magnesium Transporter Gene OsMGT1 Is Required for Conferring Aluminum Tolerance in Rice 査読

    Zhi Chang Chen, Naoki Yamaji, Ritsuko Motoyama, Yoshiaki Nagamura, Jian Feng Ma

    PLANT PHYSIOLOGY   159 ( 4 )   1624 - 1633   2012年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Magnesium (Mg)-mediated alleviation of aluminum (Al) toxicity has been observed in a number of plant species, but the mechanisms underlying the alleviation are still poorly understood. When a putative rice (Oryza sativa) Mg transporter gene, Oryza sativa MAGNESIUM TRANSPORTER1 (OsMGT1), was knocked out, the tolerance to Al, but not to cadmium and lanthanum, was decreased. However, this inhibition could be rescued by addition of 10 mu M Mg, but not by the same concentration of barium or strontium. OsMGT1 was expressed in both the roots and shoots in the absence of Al, but the expression only in the roots was rapidly up-regulated by Al. Furthermore, the expression did not respond to low pH and other metals including cadmium and lanthanum, and was regulated by an Al-responsive transcription factor, AL RESISTANCE TRANSCRIPTION FACTOR1. An investigation of subcellular localization showed that OsMGT1 was localized to the plasma membrane. A short-term (30 min) uptake experiment with stable isotope Mg-25 showed that knockout of OsMGT1 resulted in decreased Mg uptake, but that the uptake in the wild type was enhanced by Al. Mg concentration in the cell sap of the root tips was also increased in the wild-type rice, but not in the knockout lines in the presence of Al. A microarray analysis showed that transcripts of genes related to stress were more up- and down-regulated in the knockout lines. Taken together, our results indicate that OsMGT1 is a transporter for Mg uptake in the roots and that up-regulation of this gene is required for conferring Al tolerance in rice by increasing Mg concentration in the cell.

    DOI: 10.1104/pp.112.199778

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  • Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation 査読

    Matthew J. Milner, Eric Craft, Naoki Yamaji, Emi Koyama, Jian Feng Ma, Leon V. Kochian

    NEW PHYTOLOGIST   195 ( 1 )   113 - 123   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the zinc (Zn)/cadmium (Cd) hyperaccumulating plant species, Noccaea caerulescens, formerly known as Thlaspi caerulescens. NcZNT1 was previously suggested to be the primary root Zn/Cd uptake transporter. Both a characterization of NcZNT1 transport function in planta and in heterologous systems, and an analysis of NcZNT1 gene expression and NcZNT1 protein localization were carried out. We show that NcZNT1 is not only expressed in the root epidermis, but also is highly expressed in the root and shoot vasculature, suggesting a role in long-distance metal transport. Also, NcZNT1 was found to be a plasma membrane transporter that mediates Zn but not Cd, iron (Fe), manganese (Mn) or copper (Cu) uptake into plant cells. Two novel regions of the NcZNT1 promoter were identified which may be involved in both the hyperexpression of NcZNT1 and its ability to be regulated by plant Zn status. In conclusion, we demonstrate here that NcZNT1 plays a role in Zn and not Cd uptake from the soil, and based on its strong expression in the root and shoot vasculature, could be involved in long-distance transport of Zn from the root to the shoot via the xylem.

    DOI: 10.1111/j.1469-8137.2012.04144.x

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  • Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation 査読

    Matthew J. Milner, Eric Craft, Naoki Yamaji, Emi Koyama, Jian Feng Ma, Leon V. Kochian

    NEW PHYTOLOGIST   195 ( 1 )   113 - 123   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the zinc (Zn)/cadmium (Cd) hyperaccumulating plant species, Noccaea caerulescens, formerly known as Thlaspi caerulescens. NcZNT1 was previously suggested to be the primary root Zn/Cd uptake transporter. Both a characterization of NcZNT1 transport function in planta and in heterologous systems, and an analysis of NcZNT1 gene expression and NcZNT1 protein localization were carried out. We show that NcZNT1 is not only expressed in the root epidermis, but also is highly expressed in the root and shoot vasculature, suggesting a role in long-distance metal transport. Also, NcZNT1 was found to be a plasma membrane transporter that mediates Zn but not Cd, iron (Fe), manganese (Mn) or copper (Cu) uptake into plant cells. Two novel regions of the NcZNT1 promoter were identified which may be involved in both the hyperexpression of NcZNT1 and its ability to be regulated by plant Zn status. In conclusion, we demonstrate here that NcZNT1 plays a role in Zn and not Cd uptake from the soil, and based on its strong expression in the root and shoot vasculature, could be involved in long-distance transport of Zn from the root to the shoot via the xylem.

    DOI: 10.1111/j.1469-8137.2012.04144.x

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  • Nramp5 Is a Major Transporter Responsible for Manganese and Cadmium Uptake in Rice 査読

    Akimasa Sasaki, Naoki Yamaji, Kengo Yokosho, Jian Feng Ma

    PLANT CELL   24 ( 5 )   2155 - 2167   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Paddy rice (Oryza sativa) is able to accumulate high concentrations of Mn without showing toxicity; however, the molecular mechanisms underlying Mn uptake are unknown. Here, we report that a member of the Nramp (for the Natural Resistance-Associated Macrophage Protein) family, Nramp5, is involved in Mn uptake and subsequently the accumulation of high concentrations of Mn in rice. Nramp5 was constitutively expressed in the roots and encodes a plasma membrane-localized protein. Nramp5 was polarly localized at the distal side of both exodermis and endodermis cells. Knockout of Nramp5 resulted in a significant reduction in growth and grain yield, especially when grown at low Mn concentrations. This growth reduction could be partially rescued by supplying high concentrations of Mn but not by the addition of Fe. Mineral analysis showed that the concentration of Mn and Cd in both the roots and shoots was lower in the knockout line than in wild-type rice. A short-term uptake experiment revealed that the knockout line lost the ability to take up Mn and Cd. Taken together, Nramp5 is a major transporter of Mn and Cd and is responsible for the transport of Mn and Cd from the external solution to root cells.

    DOI: 10.1105/tpc.112.096925

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  • Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene 査読

    Jonatan Montpetit, Julien Vivancos, Namiki Mitani-Ueno, Naoki Yamaji, Wilfried Remus-Borel, Francois Belzile, Jian Feng Ma, Richard R. Belanger

    PLANT MOLECULAR BIOLOGY   79 ( 1-2 )   35 - 46   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Silicon (Si) is known to be beneficial to plants, namely in alleviating biotic and abiotic stresses. The magnitude of such positive effects is associated with a plant's natural ability to absorb Si. Many grasses can accumulate as much as 10% on a dry weight basis while most dicots, including Arabidopsis, will accumulate less than 0.1%. In this report, we describe the cloning and functional characterization of TaLsi1, a wheat Si transporter gene. In addition, we developed a heterologous system for the study of Si uptake in plants by introducing TaLsi1 and OsLsi1, its ortholog in rice, into Arabidopsis, a species with a very low innate Si uptake capacity. When expressed constitutively under the control of the CaMV 35S promoter, both TaLsi1 and OsLsi1 were expressed in cells of roots and shoots. Such constitutive expression of TaLsi1 or OsLsi1 resulted in a fourfold to fivefold increase in Si accumulation in transformed plants compared to WT. However, this Si absorption caused deleterious symptoms. When the wheat transporter was expressed under the control of a root-specific promoter (a boron transporter gene (AtNIP5;1) promoter), a similar increase in Si absorption was noted but the plants did not exhibit symptoms and grew normally. These results demonstrate that TaLsi1 is indeed a functional Si transporter as its expression in Arabidopsis leads to increased Si uptake, but that this expression must be confined to root cells for healthy plant development. The availability of this heterologous expression system will facilitate further studies into the mechanisms and benefits of Si uptake.

    DOI: 10.1007/s11103-012-9892-3

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  • Nramp5 Is a Major Transporter Responsible for Manganese and Cadmium Uptake in Rice 査読

    Akimasa Sasaki, Naoki Yamaji, Kengo Yokosho, Jian Feng Ma

    PLANT CELL   24 ( 5 )   2155 - 2167   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Paddy rice (Oryza sativa) is able to accumulate high concentrations of Mn without showing toxicity; however, the molecular mechanisms underlying Mn uptake are unknown. Here, we report that a member of the Nramp (for the Natural Resistance-Associated Macrophage Protein) family, Nramp5, is involved in Mn uptake and subsequently the accumulation of high concentrations of Mn in rice. Nramp5 was constitutively expressed in the roots and encodes a plasma membrane-localized protein. Nramp5 was polarly localized at the distal side of both exodermis and endodermis cells. Knockout of Nramp5 resulted in a significant reduction in growth and grain yield, especially when grown at low Mn concentrations. This growth reduction could be partially rescued by supplying high concentrations of Mn but not by the addition of Fe. Mineral analysis showed that the concentration of Mn and Cd in both the roots and shoots was lower in the knockout line than in wild-type rice. A short-term uptake experiment revealed that the knockout line lost the ability to take up Mn and Cd. Taken together, Nramp5 is a major transporter of Mn and Cd and is responsible for the transport of Mn and Cd from the external solution to root cells.

    DOI: 10.1105/tpc.112.096925

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  • Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene 査読

    Jonatan Montpetit, Julien Vivancos, Namiki Mitani-Ueno, Naoki Yamaji, Wilfried Remus-Borel, Francois Belzile, Jian Feng Ma, Richard R. Belanger

    PLANT MOLECULAR BIOLOGY   79 ( 1-2 )   35 - 46   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Silicon (Si) is known to be beneficial to plants, namely in alleviating biotic and abiotic stresses. The magnitude of such positive effects is associated with a plant's natural ability to absorb Si. Many grasses can accumulate as much as 10% on a dry weight basis while most dicots, including Arabidopsis, will accumulate less than 0.1%. In this report, we describe the cloning and functional characterization of TaLsi1, a wheat Si transporter gene. In addition, we developed a heterologous system for the study of Si uptake in plants by introducing TaLsi1 and OsLsi1, its ortholog in rice, into Arabidopsis, a species with a very low innate Si uptake capacity. When expressed constitutively under the control of the CaMV 35S promoter, both TaLsi1 and OsLsi1 were expressed in cells of roots and shoots. Such constitutive expression of TaLsi1 or OsLsi1 resulted in a fourfold to fivefold increase in Si accumulation in transformed plants compared to WT. However, this Si absorption caused deleterious symptoms. When the wheat transporter was expressed under the control of a root-specific promoter (a boron transporter gene (AtNIP5;1) promoter), a similar increase in Si absorption was noted but the plants did not exhibit symptoms and grew normally. These results demonstrate that TaLsi1 is indeed a functional Si transporter as its expression in Arabidopsis leads to increased Si uptake, but that this expression must be confined to root cells for healthy plant development. The availability of this heterologous expression system will facilitate further studies into the mechanisms and benefits of Si uptake.

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  • Acquisition of aluminium tolerance by modification of a single gene in barley. 査読 国際誌

    Miho Fujii, Kengo Yokosho, Naoki Yamaji, Daisuke Saisho, Miki Yamane, Hirokazu Takahashi, Kazuhiro Sato, Mikio Nakazono, Jian Feng Ma

    Nature communications   3   713 - 713   2012年3月

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    記述言語:英語  

    Originating from the Fertile Crescent in the Middle East, barley has now been cultivated widely on different soil types including acid soils, where aluminium toxicity is a major limiting factor. Here we show that the adaptation of barley to acid soils is achieved by the modification of a single gene (HvAACT1) encoding a citrate transporter. We find that the primary function of this protein is to release citrate from the root pericycle cells to the xylem to facilitate the translocation of iron from roots to shoots. However, a 1-kb insertion in the upstream of the HvAACT1 coding region occurring only in the Al-tolerant accessions, enhances its expression and alters the location of expression to the root tips. The altered HvAACT1 has an important role in detoxifying aluminium by secreting citrate to the rhizosphere. Thus, the insertion of a 1-kb sequence in the HvAACT1 upstream enables barley to adapt to acidic soils.

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  • Acquisition of aluminium tolerance by modification of a single gene in barley. 査読 国際誌

    Miho Fujii, Kengo Yokosho, Naoki Yamaji, Daisuke Saisho, Miki Yamane, Hirokazu Takahashi, Kazuhiro Sato, Mikio Nakazono, Jian Feng Ma

    Nature communications   3   713 - 713   2012年3月

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    記述言語:英語  

    Originating from the Fertile Crescent in the Middle East, barley has now been cultivated widely on different soil types including acid soils, where aluminium toxicity is a major limiting factor. Here we show that the adaptation of barley to acid soils is achieved by the modification of a single gene (HvAACT1) encoding a citrate transporter. We find that the primary function of this protein is to release citrate from the root pericycle cells to the xylem to facilitate the translocation of iron from roots to shoots. However, a 1-kb insertion in the upstream of the HvAACT1 coding region occurring only in the Al-tolerant accessions, enhances its expression and alters the location of expression to the root tips. The altered HvAACT1 has an important role in detoxifying aluminium by secreting citrate to the rhizosphere. Thus, the insertion of a 1-kb sequence in the HvAACT1 upstream enables barley to adapt to acidic soils.

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  • A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice 査読

    Chao-Feng Huang, Naoki Yamaji, Zhichang Chen, Jian Feng Ma

    PLANT JOURNAL   69 ( 5 )   857 - 867   2012年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Toxic aluminum enters the root cells rapidly, therefore internal detoxification is required. However, the molecular mechanisms underlying this process are poorly understood. Here we functionally characterized a rice gene, Os03g0755100 (OsALS1), that is regulated by ART1, a C2H2-type zinc finger transcription factor. OsALS1 encodes a half-size ABC transporter that is a member of the TAP (transporter associated with antigen processing) sub-group. Expression of OsALS1 was rapidly and specifically induced by Al in the roots, but not by other metals or low pH. OsALS1 was localized at all cells of the roots. Furthermore, OsALS1 is localized to the tonoplast. These expression patterns and cell specificity of localization are different from those of the homologous gene AtALS1 in Arabidopsis. Knockout of OsALS1 in three independent lines resulted in significant increased sensitivity to Al, but did not affect the sensitivity to other metals and low pH. Comparison of Al accumulation patterns between wild-type and osals1 mutants showed that there was no difference in Al levels in the cell sap of root tips between wild-type and the mutants, but the mutants accumulated more Al in the cytosol and nucleus than the wild-type. Expression of OsALS1 in yeast resulted in increased Al sensitivity due to mis-localization. These results indicate that OsALS1 localized at the tonoplast is responsible for sequestration of Al into the vacuoles, which is required for internal detoxification of Al in rice.

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  • A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice 査読

    Chao-Feng Huang, Naoki Yamaji, Zhichang Chen, Jian Feng Ma

    PLANT JOURNAL   69 ( 5 )   857 - 867   2012年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Toxic aluminum enters the root cells rapidly, therefore internal detoxification is required. However, the molecular mechanisms underlying this process are poorly understood. Here we functionally characterized a rice gene, Os03g0755100 (OsALS1), that is regulated by ART1, a C2H2-type zinc finger transcription factor. OsALS1 encodes a half-size ABC transporter that is a member of the TAP (transporter associated with antigen processing) sub-group. Expression of OsALS1 was rapidly and specifically induced by Al in the roots, but not by other metals or low pH. OsALS1 was localized at all cells of the roots. Furthermore, OsALS1 is localized to the tonoplast. These expression patterns and cell specificity of localization are different from those of the homologous gene AtALS1 in Arabidopsis. Knockout of OsALS1 in three independent lines resulted in significant increased sensitivity to Al, but did not affect the sensitivity to other metals and low pH. Comparison of Al accumulation patterns between wild-type and osals1 mutants showed that there was no difference in Al levels in the cell sap of root tips between wild-type and the mutants, but the mutants accumulated more Al in the cytosol and nucleus than the wild-type. Expression of OsALS1 in yeast resulted in increased Al sensitivity due to mis-localization. These results indicate that OsALS1 localized at the tonoplast is responsible for sequestration of Al into the vacuoles, which is required for internal detoxification of Al in rice.

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  • A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice 査読

    Chao-Feng Huang, Naoki Yamaji, Zhichang Chen, Jian Feng Ma

    Plant Journal   69 ( 5 )   857 - 867   2012年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Toxic aluminum enters the root cells rapidly, therefore internal detoxification is required. However, the molecular mechanisms underlying this process are poorly understood. Here we functionally characterized a rice gene, Os03g0755100 (OsALS1), that is regulated by ART1, a C2H2-type zinc finger transcription factor. OsALS1 encodes a half-size ABC transporter that is a member of the TAP (transporter associated with antigen processing) sub-group. Expression of OsALS1 was rapidly and specifically induced by Al in the roots, but not by other metals or low pH. OsALS1 was localized at all cells of the roots. Furthermore, OsALS1 is localized to the tonoplast. These expression patterns and cell specificity of localization are different from those of the homologous gene AtALS1 in Arabidopsis. Knockout of OsALS1 in three independent lines resulted in significant increased sensitivity to Al, but did not affect the sensitivity to other metals and low pH. Comparison of Al accumulation patterns between wild-type and osals1 mutants showed that there was no difference in Al levels in the cell sap of root tips between wild-type and the mutants, but the mutants accumulated more Al in the cytosol and nucleus than the wild-type. Expression of OsALS1 in yeast resulted in increased Al sensitivity due to mis-localization. These results indicate that OsALS1 localized at the tonoplast is responsible for sequestration of Al into the vacuoles, which is required for internal detoxification of Al in rice. © 2011 Blackwell Publishing Ltd.

    DOI: 10.1111/j.1365-313X.2011.04837.x

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  • A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots 査読

    Chao-Feng Huang, Naoki Yamaji, Kazuko Ono, Jian Feng Ma

    PLANT JOURNAL   69 ( 4 )   565 - 576   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Root outer cell layers of Oryza sativa (rice), which comprise the epidermis, exodermis and sclerenchyma, play an important role in protecting the roots from various stresses in soil, but the molecular mechanisms for the specification of these cell layers are poorly understood. In this work, we report on defective in outer cell layer specification 1 (Docs1), which is involved in the specification of outer cell layers in rice roots. Docs1 was isolated by map-based cloning using a mutant (c68) defective in the outer cell layers of primary roots. It encodes a leucine-rich repeat receptor-like kinase (LRR RLK). Docs1 mRNA was expressed in all tissues including roots, leaf blades and sheaths, and flowers. Immunostaining with an anti-Docs1 antibody showed that Docs1 was localized at the epidermis and exodermis, depending on the root region. Furthermore, Docs1 showed polar localization at the distal side. Subcellular examination showed that Docs1 was localized to the plasma membrane. Comparison of genome-wide transcriptional profiles between the wild-type and the knock-out mutant roots using microarray analysis showed that 61 and 41 genes were up- and downregulated in the mutant, including genes encoding putative transcription factors and genes potentially involved in cell wall metabolism. These results suggest that Docs1 might directly or indirectly regulate multiple genes involved in the proper development of root outer cell layers in rice.

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  • A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots 査読

    Chao-Feng Huang, Naoki Yamaji, Kazuko Ono, Jian Feng Ma

    PLANT JOURNAL   69 ( 4 )   565 - 576   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Root outer cell layers of Oryza sativa (rice), which comprise the epidermis, exodermis and sclerenchyma, play an important role in protecting the roots from various stresses in soil, but the molecular mechanisms for the specification of these cell layers are poorly understood. In this work, we report on defective in outer cell layer specification 1 (Docs1), which is involved in the specification of outer cell layers in rice roots. Docs1 was isolated by map-based cloning using a mutant (c68) defective in the outer cell layers of primary roots. It encodes a leucine-rich repeat receptor-like kinase (LRR RLK). Docs1 mRNA was expressed in all tissues including roots, leaf blades and sheaths, and flowers. Immunostaining with an anti-Docs1 antibody showed that Docs1 was localized at the epidermis and exodermis, depending on the root region. Furthermore, Docs1 showed polar localization at the distal side. Subcellular examination showed that Docs1 was localized to the plasma membrane. Comparison of genome-wide transcriptional profiles between the wild-type and the knock-out mutant roots using microarray analysis showed that 61 and 41 genes were up- and downregulated in the mutant, including genes encoding putative transcription factors and genes potentially involved in cell wall metabolism. These results suggest that Docs1 might directly or indirectly regulate multiple genes involved in the proper development of root outer cell layers in rice.

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  • OsYSL6 Is Involved in the Detoxification of Excess Manganese in Rice 査読

    Akimasa Sasaki, Naoki Yamaji, Jixing Xia, Jian Feng Ma

    PLANT PHYSIOLOGY   157 ( 4 )   1832 - 1840   2011年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Yellow Stripe-Like (YSL) proteins belong to the oligopeptide transporter family and have been implicated in metal transport and homeostasis in different plant species. Here, we functionally characterized a rice (Oryza sativa) YSL member, OsYSL6. Knockout of OsYSL6 resulted in decreased growth of both roots and shoots only in the high-manganese (Mn) condition. There was no difference in the concentration of total Mn and other essential metals between the wild-type rice and the knockout line, but the knockout line showed a higher Mn concentration in the leaf apoplastic solution and a lower Mn concentration in the symplastic solution than wild-type rice. OsYSL6 was constitutively expressed in both the shoots and roots, and the expression level was not affected by either deficiency or toxicity of various metals. Furthermore, the expression level increased with leaf age. Analysis with OsYSL6 promoter-green fluorescent protein transgenic rice revealed that OsYSL6 was expressed in all cells of both the roots and shoots. Heterogolous expression of OsYSL6 in yeast showed transport activity for the Mn-nicotianamine complex but not for the Mn-mugineic acid complex. Taken together, our results suggest that OsYSL6 is a Mn-nicotianamine transporter that is required for the detoxification of excess Mn in rice.

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  • An Al-inducible MATE gene is involved in external detoxification of Al in rice 査読

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    PLANT JOURNAL   68 ( 6 )   1061 - 1069   2011年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    A number of plant species, including rice, secretes citrate from roots in response to Al stress. Here we characterized the functions of a gene, OsFRDL4 (Os01g0919100) that belongs to the multidrug and toxic compound extrusion (MATE) family in rice (Oryza sativa). Heterologous expression in Xenopus oocyte showed that the OsFRDL4 protein was able to transport citrate and was activated by Al. The expression level of the OsFRDL4 gene in roots was very low in the absence of Al, but was greatly enhanced by Al after short exposure. Furthermore, the OsFRDL4 expression was regulated by ART1, a C2H2-type zinc finger transcription factor for Al tolerance. Transient expression of OsFRDL4 in onion epidermal cells showed that it localized to the plasma membrane. Immunostaining showed that OsFRDL4 was localized in all cells in the root tip. These expression patterns and cell specificity of localization of OsFRDL4 are different from other MATE members identified previously. Knockout of OsFRDL4 resulted in decreased Al tolerance and decreased citrate secretion compared with the wild-type rice, but did not affect citrate concentration in the xylem sap. Furthermore, there is a positive correlation between OsFRDL4 expression level and the amount of citrate secretion in rice cultivars that are differing in Al tolerance. Taken together, our results show that OsFRDL4 is an Al-induced citrate transporter localized at the plasma membrane of rice root cells and is one of the components of high Al tolerance in rice.

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  • OsYSL6 Is Involved in the Detoxification of Excess Manganese in Rice 査読

    Akimasa Sasaki, Naoki Yamaji, Jixing Xia, Jian Feng Ma

    PLANT PHYSIOLOGY   157 ( 4 )   1832 - 1840   2011年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Yellow Stripe-Like (YSL) proteins belong to the oligopeptide transporter family and have been implicated in metal transport and homeostasis in different plant species. Here, we functionally characterized a rice (Oryza sativa) YSL member, OsYSL6. Knockout of OsYSL6 resulted in decreased growth of both roots and shoots only in the high-manganese (Mn) condition. There was no difference in the concentration of total Mn and other essential metals between the wild-type rice and the knockout line, but the knockout line showed a higher Mn concentration in the leaf apoplastic solution and a lower Mn concentration in the symplastic solution than wild-type rice. OsYSL6 was constitutively expressed in both the shoots and roots, and the expression level was not affected by either deficiency or toxicity of various metals. Furthermore, the expression level increased with leaf age. Analysis with OsYSL6 promoter-green fluorescent protein transgenic rice revealed that OsYSL6 was expressed in all cells of both the roots and shoots. Heterogolous expression of OsYSL6 in yeast showed transport activity for the Mn-nicotianamine complex but not for the Mn-mugineic acid complex. Taken together, our results suggest that OsYSL6 is a Mn-nicotianamine transporter that is required for the detoxification of excess Mn in rice.

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  • An Al-inducible MATE gene is involved in external detoxification of Al in rice 査読

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    PLANT JOURNAL   68 ( 6 )   1061 - 1069   2011年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    A number of plant species, including rice, secretes citrate from roots in response to Al stress. Here we characterized the functions of a gene, OsFRDL4 (Os01g0919100) that belongs to the multidrug and toxic compound extrusion (MATE) family in rice (Oryza sativa). Heterologous expression in Xenopus oocyte showed that the OsFRDL4 protein was able to transport citrate and was activated by Al. The expression level of the OsFRDL4 gene in roots was very low in the absence of Al, but was greatly enhanced by Al after short exposure. Furthermore, the OsFRDL4 expression was regulated by ART1, a C2H2-type zinc finger transcription factor for Al tolerance. Transient expression of OsFRDL4 in onion epidermal cells showed that it localized to the plasma membrane. Immunostaining showed that OsFRDL4 was localized in all cells in the root tip. These expression patterns and cell specificity of localization of OsFRDL4 are different from other MATE members identified previously. Knockout of OsFRDL4 resulted in decreased Al tolerance and decreased citrate secretion compared with the wild-type rice, but did not affect citrate concentration in the xylem sap. Furthermore, there is a positive correlation between OsFRDL4 expression level and the amount of citrate secretion in rice cultivars that are differing in Al tolerance. Taken together, our results show that OsFRDL4 is an Al-induced citrate transporter localized at the plasma membrane of rice root cells and is one of the components of high Al tolerance in rice.

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  • A functional cutin matrix is required for plant protection against water loss 査読

    Guoxiong Chen, Takao Komatsuda, Jian Feng Ma, Chao Li, Naoki Yamaji, Eviatar Nevo

    Plant Signaling and Behavior   6 ( 9 )   1297 - 1299   2011年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The plant cuticle, a cutin matrix embedded with and covered by wax, seals the aerial organ's surface to protect the plant against uncontrolled water loss. The cutin matrix is essential for the cuticle to function as a barrier to water loss. Recently, we identified from wild barley a drought supersensitive mutant, eibi1, which is caused by a defective cutin matrix as the result of the loss of function of HvABCG31, an ABCG full transporter. Here, we report that eibi1 epidermal cells contain lipid-like droplets, which are supposed to consist of cutin monomers that have not been transported out of the cells. The eibi1 cuticle is fragile due to a defective cutin matrix. The rice ortholog of the EIBI1 gene has a similar pattern of expression, young shoot but not flag leaf blade, as the barley gene. The model of the function of Eibi1 is discussed. The HvABCG31 full transporter functions in the export of cutin components and contributed to land plant colonization, hence also to terrestrial life evolution. © 2011 Landes Bioscience.

    DOI: 10.4161/psb.6.9.17507

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  • The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic 査読

    Namiki Mitani-Ueno, Naoki Yamaji, Fang-Jie Zhao, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   62 ( 12 )   4391 - 4398   2011年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Nodulin-26-like intrinsic proteins (NIPs) of the aquaporin family are involved in the transport of diverse solutes, but the mechanisms controlling the selectivity of transport substrates are poorly understood. The purpose of this study was to investigate how the aromatic/arginine (ar/R) selectivity filter influences the substrate selectivity of two NIP aquaporins; the silicic acid (Si) transporter OsLsi1 (OsNIP2;1) from rice and the boric acid (B) transporter AtNIP5;1 from Arabidopsis; both proteins are also permeable to arsenite. Native and site-directed mutagenized variants of the two genes were expressed in Xenopus oocytes and the transport activities for Si, B, arsenite, and water were assayed. Substitution of the amino acid at the ar/R second helix (H2) position of OsLsi1 did not affect the transport activities for Si, B, and arsenite, but that at the H5 position resulted in a total loss of Si and B transport activities and a partial loss of arsenite transport activity. Conversely, changes of the AtNIP5;1 ar/R selectivity filter and the NPA motifs to the OsLsi1 type did not result in a gain of Si transport activity. B transport activity was partially lost in the H5 mutant but unaffected in the H2 mutant of AtNIP5;1. In contrast, both the single and double mutations at the H2 and/or H5 positions of AtNIP5;1 did not affect arsenite transport activity. The results reveal that the residue at the H5 position of the ar/R filter of both OsLsi1 and AtNIP5;1 plays a key role in the permeability to Si and B, but there is a relatively low selectivity for arsenite.

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  • The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic 査読

    Namiki Mitani-Ueno, Naoki Yamaji, Fang-Jie Zhao, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   62 ( 12 )   4391 - 4398   2011年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Nodulin-26-like intrinsic proteins (NIPs) of the aquaporin family are involved in the transport of diverse solutes, but the mechanisms controlling the selectivity of transport substrates are poorly understood. The purpose of this study was to investigate how the aromatic/arginine (ar/R) selectivity filter influences the substrate selectivity of two NIP aquaporins; the silicic acid (Si) transporter OsLsi1 (OsNIP2;1) from rice and the boric acid (B) transporter AtNIP5;1 from Arabidopsis; both proteins are also permeable to arsenite. Native and site-directed mutagenized variants of the two genes were expressed in Xenopus oocytes and the transport activities for Si, B, arsenite, and water were assayed. Substitution of the amino acid at the ar/R second helix (H2) position of OsLsi1 did not affect the transport activities for Si, B, and arsenite, but that at the H5 position resulted in a total loss of Si and B transport activities and a partial loss of arsenite transport activity. Conversely, changes of the AtNIP5;1 ar/R selectivity filter and the NPA motifs to the OsLsi1 type did not result in a gain of Si transport activity. B transport activity was partially lost in the H5 mutant but unaffected in the H2 mutant of AtNIP5;1. In contrast, both the single and double mutations at the H2 and/or H5 positions of AtNIP5;1 did not affect arsenite transport activity. The results reveal that the residue at the H5 position of the ar/R filter of both OsLsi1 and AtNIP5;1 plays a key role in the permeability to Si and B, but there is a relatively low selectivity for arsenite.

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  • Transport of silicon from roots to panicles in plants 査読

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani-Ueno

    PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES   87 ( 7 )   377 - 385   2011年7月

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    記述言語:英語   出版者・発行元:JAPAN ACAD  

    Silicon (Si) is the most abundant minerals in soil and exerts beneficial effects on plant growth by alleviating various stresses. The transport of Si from soil to the panicles is mediated by different transporters. Lsi1, belonging to a NIP group of the aquaporin family, is responsible for the uptake of Si from soil into the root cells in both dicots and monocots although its expression patterns and cellular localization differ with plant species. The subsequent transport of Si out of the root cells towards the stele is medicated by an active efflux transporter, Lsi2. Lsi1. and Lsi2 are polarly localized at the distal and proximal sides, respectively, of both exodermis and endodermis in rice root. Silicon in the xylem sap is presented in the form of monosilicic acid and is unloaded by Lsi6, a homolog of Lsi1 in rice. Lsi6 is also involved in the inter-vascular transfer of Si at the node, which is necessary for preferential Si distribution to the panicles.

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  • An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice 査読

    Guoxiong Chen, Takao Komatsuda, Jian Feng Ma, Christiane Nawrath, Mohammad Pourkheirandish, Akemi Tagiri, Yin-Gang Hu, Mohammad Sameri, Xinrong Li, Xin Zhao, Yubing Liu, Chao Li, Xiaoying Ma, Aidong Wang, Sudha Nair, Ning Wang, Akio Miyao, Shun Sakuma, Naoki Yamaji, Xiuting Zheng, Eviatar Nevo

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   108 ( 30 )   12354 - 12359   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Land plants have developed a cuticle preventing uncontrolled water loss. Here we report that an ATP-binding cassette (ABC) subfamily G (ABCG) full transporter is required for leaf water conservation in both wild barley and rice. A spontaneous mutation, eibi1.b, in wild barley has a low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. Map-based cloning revealed that Eibi1 encodes an HvABCG31 full transporter. The gene was highly expressed in the elongation zone of a growing leaf (the site of cutin synthesis), and its gene product also was localized in developing, but not in mature tissue. A de novo wild barley mutant named "eibi1.c," along with two transposon insertion lines of rice mutated in the ortholog of HvABCG31 also were unable to restrict water loss from detached leaves. HvABCG31 is hypothesized to function as a transporter involved in cutin formation. Homologs of HvABCG31 were found in green algae, moss, and lycopods, indicating that this full transporter is highly conserved in the evolution of land plants.

    DOI: 10.1073/pnas.1108444108

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  • An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice 査読

    Guoxiong Chen, Takao Komatsuda, Jian Feng Ma, Christiane Nawrath, Mohammad Pourkheirandish, Akemi Tagiri, Yin-Gang Hu, Mohammad Sameri, Xinrong Li, Xin Zhao, Yubing Liu, Chao Li, Xiaoying Ma, Aidong Wang, Sudha Nair, Ning Wang, Akio Miyao, Shun Sakuma, Naoki Yamaji, Xiuting Zheng, Eviatar Nevo

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   108 ( 30 )   12354 - 12359   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Land plants have developed a cuticle preventing uncontrolled water loss. Here we report that an ATP-binding cassette (ABC) subfamily G (ABCG) full transporter is required for leaf water conservation in both wild barley and rice. A spontaneous mutation, eibi1.b, in wild barley has a low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. Map-based cloning revealed that Eibi1 encodes an HvABCG31 full transporter. The gene was highly expressed in the elongation zone of a growing leaf (the site of cutin synthesis), and its gene product also was localized in developing, but not in mature tissue. A de novo wild barley mutant named "eibi1.c," along with two transposon insertion lines of rice mutated in the ortholog of HvABCG31 also were unable to restrict water loss from detached leaves. HvABCG31 is hypothesized to function as a transporter involved in cutin formation. Homologs of HvABCG31 were found in green algae, moss, and lycopods, indicating that this full transporter is highly conserved in the evolution of land plants.

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  • Transport of silicon from roots to panicles in plants 査読

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani-Ueno

    PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES   87 ( 7 )   377 - 385   2011年7月

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    記述言語:英語   出版者・発行元:JAPAN ACAD  

    Silicon (Si) is the most abundant minerals in soil and exerts beneficial effects on plant growth by alleviating various stresses. The transport of Si from soil to the panicles is mediated by different transporters. Lsi1, belonging to a NIP group of the aquaporin family, is responsible for the uptake of Si from soil into the root cells in both dicots and monocots although its expression patterns and cellular localization differ with plant species. The subsequent transport of Si out of the root cells towards the stele is medicated by an active efflux transporter, Lsi2. Lsi1. and Lsi2 are polarly localized at the distal and proximal sides, respectively, of both exodermis and endodermis in rice root. Silicon in the xylem sap is presented in the form of monosilicic acid and is unloaded by Lsi6, a homolog of Lsi1 in rice. Lsi6 is also involved in the inter-vascular transfer of Si at the node, which is necessary for preferential Si distribution to the panicles.

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  • Transport of silicon from roots to panicles in plants 査読

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani-Ueno

    PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES   87 ( 7 )   377 - 385   2011年7月

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    記述言語:英語   出版者・発行元:JAPAN ACAD  

    Silicon (Si) is the most abundant minerals in soil and exerts beneficial effects on plant growth by alleviating various stresses. The transport of Si from soil to the panicles is mediated by different transporters. Lsi1, belonging to a NIP group of the aquaporin family, is responsible for the uptake of Si from soil into the root cells in both dicots and monocots although its expression patterns and cellular localization differ with plant species. The subsequent transport of Si out of the root cells towards the stele is medicated by an active efflux transporter, Lsi2. Lsi1. and Lsi2 are polarly localized at the distal and proximal sides, respectively, of both exodermis and endodermis in rice root. Silicon in the xylem sap is presented in the form of monosilicic acid and is unloaded by Lsi6, a homolog of Lsi1 in rice. Lsi6 is also involved in the inter-vascular transfer of Si at the node, which is necessary for preferential Si distribution to the panicles.

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  • Identification of a Cis-Acting Element of ART1, a C2H2-Type Zinc-Finger Transcription Factor for Aluminum Tolerance in Rice 査読

    Tomokazu Tsutsui, Naoki Yamaji, Jian Feng Ma

    PLANT PHYSIOLOGY   156 ( 2 )   925 - 931   2011年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) is one of the most aluminum (Al)-tolerant species among small-grain cereals. Recent identification of a transcription factor AL RESISTANCE TRANSCRIPTION FACTOR1 (ART1) revealed that this high Al tolerance in rice is achieved by multiple genes involved in detoxification of Al at different cellular levels. ART1 is a C2H2-type zinc-finger transcription factor and regulates the expression of 31 genes in the downstream. In this study, we attempted to identify a cis-acting element of ART1. We used the promoter region of SENSITIVE TO AL RHIZOTOXICITY1, an Al tolerance gene in the downstream of ART1. With the help of gel-shift assay, we were able to identify the cis-acting element as GGN(T/g/a/C)V(C/A/g)S(C/G). This element was found in the promoter region of 29 genes among 31 ART1-regulated genes. To confirm this cis-acting element in vivo, we transiently introduced this element one or five times tandemly repeated sequence with 35S minimal promoter and green fluorescent protein reporter together with or without ART1 gene in the tobacco (Nicotiana tabacum) mesophyll protoplasts. The results showed that the expression of green fluorescent protein reporter responded to ART1 expression. Furthermore, the expression increased with repetition of the cis-acting element. Our results indicate that the five nucleotides identified are the target DNA-binding sequence of ART1.

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  • Elevated expression of TcHMA3 plays a key role in the extreme Cd tolerance in a Cd-hyperaccumulating ecotype of Thlaspi caerulescens 査読

    Daisei Ueno, Matthew J. Milner, Naoki Yamaji, Kengo Yokosho, Emi Koyama, M. Clemencia Zambrano, Molly Kaskie, Stephen Ebbs, Leon V. Kochian, Jian Feng Ma

    PLANT JOURNAL   66 ( 5 )   852 - 862   2011年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    P&gt;Cadmium (Cd) is a highly toxic heavy metal for plants, but several unique Cd-hyperaccumulating plant species are able to accumulate this metal to extraordinary concentrations in the aboveground tissues without showing any toxic symptoms. However, the molecular mechanisms underlying this hypertolerance to Cd are poorly understood. Here we have isolated and functionally characterized an allelic gene, TcHMA3 (heavy metal ATPase 3) from two ecotypes (Ganges and Prayon) of Thlaspi caerulescens contrasting in Cd accumulation and tolerance. The TcHMA3 alleles from the higher (Ganges) and lower Cd-accumulating ecotype (Prayon) share 97.8% identity, and encode a P(1B)-type ATPase. There were no differences in the expression pattern, cell-specificity of protein localization and transport substrate-specificity of TcHMA3 between the two ecotypes. Both alleles were characterized by constitutive expression in the shoot and root, a tonoplast localization of the protein in all leaf cells and specific transport activity for Cd. The only difference between the two ecotypes was the expression level of TcHMA3: Ganges showed a sevenfold higher expression than Prayon, partly caused by a higher copy number. Furthermore, the expression level and localization of TcHMA3 were different from AtHMA3 expression in Arabidopsis. Overexpression of TcHMA3 in Arabidopsis significantly enhanced tolerance to Cd and slightly increased tolerance to Zn, but did not change Co or Pb tolerance. These results indicate that TcHMA3 is a tonoplast-localized transporter highly specific for Cd, which is responsible for sequestration of Cd into the leaf vacuoles, and that a higher expression of this gene is required for Cd hypertolerance in the Cd-hyperaccumulating ecotype of T. caerulescens.

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  • Elevated expression of TcHMA3 plays a key role in the extreme Cd tolerance in a Cd-hyperaccumulating ecotype of Thlaspi caerulescens 査読

    Daisei Ueno, Matthew J. Milner, Naoki Yamaji, Kengo Yokosho, Emi Koyama, M. Clemencia Zambrano, Molly Kaskie, Stephen Ebbs, Leon V. Kochian, Jian Feng Ma

    PLANT JOURNAL   66 ( 5 )   852 - 862   2011年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    P&gt;Cadmium (Cd) is a highly toxic heavy metal for plants, but several unique Cd-hyperaccumulating plant species are able to accumulate this metal to extraordinary concentrations in the aboveground tissues without showing any toxic symptoms. However, the molecular mechanisms underlying this hypertolerance to Cd are poorly understood. Here we have isolated and functionally characterized an allelic gene, TcHMA3 (heavy metal ATPase 3) from two ecotypes (Ganges and Prayon) of Thlaspi caerulescens contrasting in Cd accumulation and tolerance. The TcHMA3 alleles from the higher (Ganges) and lower Cd-accumulating ecotype (Prayon) share 97.8% identity, and encode a P(1B)-type ATPase. There were no differences in the expression pattern, cell-specificity of protein localization and transport substrate-specificity of TcHMA3 between the two ecotypes. Both alleles were characterized by constitutive expression in the shoot and root, a tonoplast localization of the protein in all leaf cells and specific transport activity for Cd. The only difference between the two ecotypes was the expression level of TcHMA3: Ganges showed a sevenfold higher expression than Prayon, partly caused by a higher copy number. Furthermore, the expression level and localization of TcHMA3 were different from AtHMA3 expression in Arabidopsis. Overexpression of TcHMA3 in Arabidopsis significantly enhanced tolerance to Cd and slightly increased tolerance to Zn, but did not change Co or Pb tolerance. These results indicate that TcHMA3 is a tonoplast-localized transporter highly specific for Cd, which is responsible for sequestration of Cd into the leaf vacuoles, and that a higher expression of this gene is required for Cd hypertolerance in the Cd-hyperaccumulating ecotype of T. caerulescens.

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  • Identification of a Cis-Acting Element of ART1, a C2H2-Type Zinc-Finger Transcription Factor for Aluminum Tolerance in Rice 査読

    Tomokazu Tsutsui, Naoki Yamaji, Jian Feng Ma

    PLANT PHYSIOLOGY   156 ( 2 )   925 - 931   2011年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) is one of the most aluminum (Al)-tolerant species among small-grain cereals. Recent identification of a transcription factor AL RESISTANCE TRANSCRIPTION FACTOR1 (ART1) revealed that this high Al tolerance in rice is achieved by multiple genes involved in detoxification of Al at different cellular levels. ART1 is a C2H2-type zinc-finger transcription factor and regulates the expression of 31 genes in the downstream. In this study, we attempted to identify a cis-acting element of ART1. We used the promoter region of SENSITIVE TO AL RHIZOTOXICITY1, an Al tolerance gene in the downstream of ART1. With the help of gel-shift assay, we were able to identify the cis-acting element as GGN(T/g/a/C)V(C/A/g)S(C/G). This element was found in the promoter region of 29 genes among 31 ART1-regulated genes. To confirm this cis-acting element in vivo, we transiently introduced this element one or five times tandemly repeated sequence with 35S minimal promoter and green fluorescent protein reporter together with or without ART1 gene in the tobacco (Nicotiana tabacum) mesophyll protoplasts. The results showed that the expression of green fluorescent protein reporter responded to ART1 expression. Furthermore, the expression increased with repetition of the cis-acting element. Our results indicate that the five nucleotides identified are the target DNA-binding sequence of ART1.

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  • Isolation and characterization of a barley yellow stripe-like gene, HvYSL5. 査読

    Luqing Zheng, Miho Fujii, Naoki Yamaji, Akimasa Sasaki, Miki Yamane, Isamu Sakurai, Kazuhiro Sato, Jian Feng Ma

    Plant & cell physiology   52 ( 5 )   765 - 74   2011年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Yellow stripe-like (YSL) family transporters, belonging to a novel subfamily of oligopeptide transporter (OPT), has been proposed to be involved in metal uptake and long-distance transport, but only a few of them have been functionally characterized so far. In the present study, we isolated an uncharacterized member of the YSL family, HvYSL5, in barley based on expressed sequence tag (EST) information. HvYSL5 shared 50% identity with HvYS1, a transporter for the ferric-mugineic acid complex, at the amino acid level. Promoter analysis showed that the HvYSL5 upstream sequence contains both iron deficiency response element 1 and 2 (IDE1 and 2). HvYSL5 was expressed in the roots and the expression was greatly induced by Fe deficiency, but not by deficiency of other metals including Zn, Cu and Mn. Spatial investigation showed that much higher expression of HvYSL5 was found in the mature zones of the roots, but not in the root tips. Furthermore, the expression showed a diurnal rhythm, being the highest in the morning, but with no expression in the afternoon. HvYSL5 was localized in all root cells, and subcellular localization analysis showed that HvYSL5 is likely to be localized in the vesicles. Knockdown of HvYSL5 did not result in any detectable phenotype changes. Although the exact role of HvYSL5 remains to be examined, our results suggest that it is involved in the transient storage of Fe or phytosiderophores.

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  • Isolation and characterization of a barley yellow stripe-like gene, HvYSL5. 査読

    Luqing Zheng, Miho Fujii, Naoki Yamaji, Akimasa Sasaki, Miki Yamane, Isamu Sakurai, Kazuhiro Sato, Jian Feng Ma

    Plant & cell physiology   52 ( 5 )   765 - 74   2011年5月

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    記述言語:英語  

    Yellow stripe-like (YSL) family transporters, belonging to a novel subfamily of oligopeptide transporter (OPT), has been proposed to be involved in metal uptake and long-distance transport, but only a few of them have been functionally characterized so far. In the present study, we isolated an uncharacterized member of the YSL family, HvYSL5, in barley based on expressed sequence tag (EST) information. HvYSL5 shared 50% identity with HvYS1, a transporter for the ferric-mugineic acid complex, at the amino acid level. Promoter analysis showed that the HvYSL5 upstream sequence contains both iron deficiency response element 1 and 2 (IDE1 and 2). HvYSL5 was expressed in the roots and the expression was greatly induced by Fe deficiency, but not by deficiency of other metals including Zn, Cu and Mn. Spatial investigation showed that much higher expression of HvYSL5 was found in the mature zones of the roots, but not in the root tips. Furthermore, the expression showed a diurnal rhythm, being the highest in the morning, but with no expression in the afternoon. HvYSL5 was localized in all root cells, and subcellular localization analysis showed that HvYSL5 is likely to be localized in the vesicles. Knockdown of HvYSL5 did not result in any detectable phenotype changes. Although the exact role of HvYSL5 remains to be examined, our results suggest that it is involved in the transient storage of Fe or phytosiderophores.

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  • Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation 査読

    Namiki Mitani, Naoki Yamaji, Yukiko Ago, Kozo Iwasaki, Jian Feng Ma

    PLANT JOURNAL   66 ( 2 )   231 - 240   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    P&gt;A high accumulation of silicon (Si) is required for overcoming abiotic and biotic stresses, but the molecular mechanisms of Si uptake, especially in dicotyledonous species, is poorly understood. Herein, we report the identification of an influx transporter of Si in two Cucurbita moschata (pumpkin) cultivars greatly differing in Si accumulation, which are used for the rootstocks of bloom and bloomless Cucumis sativus (cucumber), respectively. Heterogeneous expression in both Xenopus oocytes and rice mutant defective in Si uptake showed that the influx transporter from the bloom pumpkin rootstock can transport Si, whereas that from the bloomless rootstock cannot. Analysis with site-directed mutagenesis showed that, among the two amino acid residues differing between the two types of rootstocks, only changing a proline to a leucine at position 242 results in the loss of Si transport activity. Furthermore, all pumpkin cultivars for bloomless rootstocks tested have this mutation. The transporter is localized in all cells of the roots, and investigation of the subcellular localization with different approaches consistently showed that the influx Si transporter from the bloom pumpkin rootstock was localized at the plasma membrane, whereas the one from the bloomless rootstock was localized at the endoplasmic reticulum. Taken together, our results indicate that the difference in Si uptake between two pumpkin cultivars is probably the result of allelic variation in one amino acid residue of the Si influx transporter, which affects the subcellular localization and subsequent transport of Si from the external solution to the root cells.

    DOI: 10.1111/j.1365-313X.2011.04483.x

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  • Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation 査読

    Namiki Mitani, Naoki Yamaji, Yukiko Ago, Kozo Iwasaki, Jian Feng Ma

    PLANT JOURNAL   66 ( 2 )   231 - 240   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    P&gt;A high accumulation of silicon (Si) is required for overcoming abiotic and biotic stresses, but the molecular mechanisms of Si uptake, especially in dicotyledonous species, is poorly understood. Herein, we report the identification of an influx transporter of Si in two Cucurbita moschata (pumpkin) cultivars greatly differing in Si accumulation, which are used for the rootstocks of bloom and bloomless Cucumis sativus (cucumber), respectively. Heterogeneous expression in both Xenopus oocytes and rice mutant defective in Si uptake showed that the influx transporter from the bloom pumpkin rootstock can transport Si, whereas that from the bloomless rootstock cannot. Analysis with site-directed mutagenesis showed that, among the two amino acid residues differing between the two types of rootstocks, only changing a proline to a leucine at position 242 results in the loss of Si transport activity. Furthermore, all pumpkin cultivars for bloomless rootstocks tested have this mutation. The transporter is localized in all cells of the roots, and investigation of the subcellular localization with different approaches consistently showed that the influx Si transporter from the bloom pumpkin rootstock was localized at the plasma membrane, whereas the one from the bloomless rootstock was localized at the endoplasmic reticulum. Taken together, our results indicate that the difference in Si uptake between two pumpkin cultivars is probably the result of allelic variation in one amino acid residue of the Si influx transporter, which affects the subcellular localization and subsequent transport of Si from the external solution to the root cells.

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  • Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan 査読

    Daisei Ueno, Emi Koyama, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   62 ( 7 )   2265 - 2272   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Cadmium (Cd) in rice is a major source of Cd intake for people on a staple rice diet. The mechanisms underlying Cd accumulation in rice plant are still poorly understood. Here, we characterized the physiology and genetics of Cd transport in a high-Cd-accumulating cultivar (Jarjan) of rice (Oryza sativa). Jarjan showed 5- to 34-fold higher Cd accumulation in the shoots and grains than the cultivar Nipponbare, when it was grown in either a non-Cd-contaminated or a Cd-contaminated soil. A short-term uptake experiment showed no significant difference in Cd uptake by the roots between the two cultivars. However, Jarjan translocated 49% of the total Cd taken up to the shoots, whereas Nipponbare retained most of the Cd in the roots. In both concentration- and time-dependent experiments, Jarjan showed a superior capacity for root-to-shoot translocation of Cd. These results indicate that the high-Cd-accumulation phenotype in Jarjan results from efficient translocation of Cd from roots to shoots. Genetic analysis using an F(2) population derived from Jarjan and Nipponbare revealed that plants showing high- and low-Cd-accumulation phenotypes segregated in a 1:3 ratio, indicating that high accumulation in Jarjan is controlled by a single recessive gene. Furthermore, we isolated OsHMA3, a gene encoding a tonoplast-localized Cd transporter from Jarjan. The OsHMA3 protein was localized in all roots cells, but the sequence has a mutation leading to loss of function. Therefore, failure to sequester Cd into the root vacuoles by OsHMA3 is probably responsible for high Cd accumulation in Jarjan.

    DOI: 10.1093/jxb/erq383

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  • Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan 査読

    Daisei Ueno, Emi Koyama, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   62 ( 7 )   2265 - 2272   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Cadmium (Cd) in rice is a major source of Cd intake for people on a staple rice diet. The mechanisms underlying Cd accumulation in rice plant are still poorly understood. Here, we characterized the physiology and genetics of Cd transport in a high-Cd-accumulating cultivar (Jarjan) of rice (Oryza sativa). Jarjan showed 5- to 34-fold higher Cd accumulation in the shoots and grains than the cultivar Nipponbare, when it was grown in either a non-Cd-contaminated or a Cd-contaminated soil. A short-term uptake experiment showed no significant difference in Cd uptake by the roots between the two cultivars. However, Jarjan translocated 49% of the total Cd taken up to the shoots, whereas Nipponbare retained most of the Cd in the roots. In both concentration- and time-dependent experiments, Jarjan showed a superior capacity for root-to-shoot translocation of Cd. These results indicate that the high-Cd-accumulation phenotype in Jarjan results from efficient translocation of Cd from roots to shoots. Genetic analysis using an F(2) population derived from Jarjan and Nipponbare revealed that plants showing high- and low-Cd-accumulation phenotypes segregated in a 1:3 ratio, indicating that high accumulation in Jarjan is controlled by a single recessive gene. Furthermore, we isolated OsHMA3, a gene encoding a tonoplast-localized Cd transporter from Jarjan. The OsHMA3 protein was localized in all roots cells, but the sequence has a mutation leading to loss of function. Therefore, failure to sequester Cd into the root vacuoles by OsHMA3 is probably responsible for high Cd accumulation in Jarjan.

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  • Further characterization of a rice silicon efflux transporter, Lsi2 査読

    Naoki Yamaji, Jian Feng Ma

    SOIL SCIENCE AND PLANT NUTRITION   57 ( 2 )   259 - 264   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    High silicon (Si) accumulation is required for high and sustainable production of rice. The uptake of Si by rice roots is mediated by both influx transporter Lsi1 and efflux transporter Lsi2. Here we further characterized Lsi2 in terms of localization and expression patterns. Analysis with promoter-GFP transgenic rice revealed that Lsi2 was expressed in the main and lateral roots, but not in the root hairs. In lateral roots, Lsi2 was localized at the proximal side of both exodermis and endodermis like main roots. The expression of Lsi2 gene was down-regulated by Si in the wild-type rice, but unaffected in the lsi2 mutant, suggesting that the expression of Lsi2 is regulated by Si accumulated in the shoots. Treatment with drought and abscisic acid rapidly resulted in decreased Lsi2 expression. Monitoring of Lsi2 expression at different growth stage showed a transient increase around the heading stage in rice grown in a paddy field. These expression patterns are similar to those of Lsi1, indicating that Lsi2 is co-regulated with Lsi1 and the expression of these two genes responds to both environmental condition and plant Si requirement.

    DOI: 10.1080/00380768.2011.565480

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  • Further characterization of a rice silicon efflux transporter, Lsi2 査読

    Naoki Yamaji, Jian Feng Ma

    SOIL SCIENCE AND PLANT NUTRITION   57 ( 2 )   259 - 264   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    High silicon (Si) accumulation is required for high and sustainable production of rice. The uptake of Si by rice roots is mediated by both influx transporter Lsi1 and efflux transporter Lsi2. Here we further characterized Lsi2 in terms of localization and expression patterns. Analysis with promoter-GFP transgenic rice revealed that Lsi2 was expressed in the main and lateral roots, but not in the root hairs. In lateral roots, Lsi2 was localized at the proximal side of both exodermis and endodermis like main roots. The expression of Lsi2 gene was down-regulated by Si in the wild-type rice, but unaffected in the lsi2 mutant, suggesting that the expression of Lsi2 is regulated by Si accumulated in the shoots. Treatment with drought and abscisic acid rapidly resulted in decreased Lsi2 expression. Monitoring of Lsi2 expression at different growth stage showed a transient increase around the heading stage in rice grown in a paddy field. These expression patterns are similar to those of Lsi1, indicating that Lsi2 is co-regulated with Lsi1 and the expression of these two genes responds to both environmental condition and plant Si requirement.

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  • Further characterization of an aluminum influx transporter in rice 査読

    Jixing Xia, Naoki Yamaji, Jian Feng Ma

    Plant Signaling and Behavior   6 ( 1 )   160 - 163   2011年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nrat1 is a plasma membrane-localized aluminum transporter recently identified in rice, which is a member of Nramp family. Here, we further characterized this transporter in terms of transport substrate specificity. Heterologous assay in yeast showed that Al transport activity by Nrat1 was unaffected by the presence of high concentration of Ca, but significantly inhibited by trivalent ions including Yb and Ga, analogs of Al. Knockout of Nrat1 did not affect the uptake of Cd and Mn in rice. On the other hand, over expression of Nrat1 led to enhanced Al uptake by rice roots compared with wild-type rice, but did not affect Cd uptake. These results provide further evidence that unlike other Nramp members, Nrat1 is an influx transporter for trivalent Al ion. © 2011 Landes Bioscience.

    DOI: 10.4161/psb.6.1.14319

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  • Further characterization of a rice silicon efflux transporter, Lsi2 査読

    Naoki Yamaji, Jian Feng Ma

    Soil Science and Plant Nutrition   57 ( 2 )   259 - 264   2011年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    High silicon (Si) accumulation is required for high and sustainable production of rice. The uptake of Si by rice roots is mediated by both influx transporter Lsi1 and efflux transporter Lsi2. Here we further characterized Lsi2 in terms of localization and expression patterns. Analysis with promoter-GFP transgenic rice revealed that Lsi2 was expressed in the main and lateral roots, but not in the root hairs. In lateral roots, Lsi2 was localized at the proximal side of both exodermis and endodermis like main roots. The expression of Lsi2 gene was down-regulated by Si in the wild-type rice, but unaffected in the lsi2 mutant, suggesting that the expression of Lsi2 is regulated by Si accumulated in the shoots. Treatment with drought and abscisic acid rapidly resulted in decreased Lsi2 expression. Monitoring of Lsi2 expression at different growth stage showed a transient increase around the heading stage in rice grown in a paddy field. These expression patterns are similar to those of Lsi1, indicating that Lsi2 is co-regulated with Lsi1 and the expression of these two genes responds to both environmental condition and plant Si requirement. © 2011 Japanese Society of Soil Science and Plant Nutrition.

    DOI: 10.1080/00380768.2011.565480

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  • Plasma membrane-localized transporter for aluminum in rice 査読

    Jixing Xia, Naoki Yamaji, Tomonari Kasai, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   107 ( 43 )   18381 - 18385   2010年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Aluminum (Al) is the most abundant metal in the Earth&apos;s crust, but its trivalent ionic form is highly toxic to all organisms at low concentrations. How Al enters cells has not been elucidated in any organisms. Herein, we report a transporter, Nrat1 (Nramp aluminum transporter 1), specific for trivalent Al ion in rice. Nrat1 belongs to the Nramp (natural resistance-associated macrophage protein) family, but shares a low similarity with other Nramp members. When expressed in yeast, Nrat1 transports trivalent Al ion, but not other divalent ions, such as manganese, iron, and cadmium, or the Al-citrate complex. Nrat1 is localized at the plasma membranes of all cells of root tips except epidermal cells. Knockout of Nrat1 resulted in decreased Al uptake, increased Al binding to cell wall, and enhanced Al sensitivity, but did not affect the tolerance to other metals. Expression of Nrat1 is up-regulated by Al in the roots and regulated by a C2H2 zinc finger transcription factor (ART1). We therefore concluded that Nrat1 is a plasma membrane-localized transporter for trivalent Al, which is required for a prior step of final Al detoxification through sequestration of Al into vacuoles.

    DOI: 10.1073/pnas.1004949107

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  • Plasma membrane-localized transporter for aluminum in rice 査読

    Jixing Xia, Naoki Yamaji, Tomonari Kasai, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   107 ( 43 )   18381 - 18385   2010年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Aluminum (Al) is the most abundant metal in the Earth&apos;s crust, but its trivalent ionic form is highly toxic to all organisms at low concentrations. How Al enters cells has not been elucidated in any organisms. Herein, we report a transporter, Nrat1 (Nramp aluminum transporter 1), specific for trivalent Al ion in rice. Nrat1 belongs to the Nramp (natural resistance-associated macrophage protein) family, but shares a low similarity with other Nramp members. When expressed in yeast, Nrat1 transports trivalent Al ion, but not other divalent ions, such as manganese, iron, and cadmium, or the Al-citrate complex. Nrat1 is localized at the plasma membranes of all cells of root tips except epidermal cells. Knockout of Nrat1 resulted in decreased Al uptake, increased Al binding to cell wall, and enhanced Al sensitivity, but did not affect the tolerance to other metals. Expression of Nrat1 is up-regulated by Al in the roots and regulated by a C2H2 zinc finger transcription factor (ART1). We therefore concluded that Nrat1 is a plasma membrane-localized transporter for trivalent Al, which is required for a prior step of final Al detoxification through sequestration of Al into vacuoles.

    DOI: 10.1073/pnas.1004949107

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  • Gene limiting cadmium accumulation in rice 査読

    Daisei Ueno, Naoki Yamaji, Izumi Kono, Chao Feng Huang, Tsuyu Ando, Masahiro Yano, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   107 ( 38 )   16500 - 16505   2010年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Intake of toxic cadmium (Cd) from rice caused Itai-itai disease in the past and it is still a threat for human health. Therefore, control of the accumulation of Cd from soil is an important food-safety issue, but the molecular mechanism for the control is unknown. Herein, we report a gene (OsHMA3) responsible for low Cd accumulation in rice that was isolated from a mapping population derived from a cross between a high and low Cd-accumulating cultivar. The gene encodes a transporter belonging to the P(1B)-type ATPase family, but shares low similarity with other members. Heterologous expression in yeast showed that the transporter from the low-Cd cultivar is functional, but the transporter from the high-Cd cultivar had lost its function, probably because of the single amino acid mutation. The transporter is mainly expressed in the tonoplast of root cells at a similar level in both the low and high Cd-accumulating cultivars. Overexpression of the functional gene from the low Cd-accumulating cultivar selectively decreased accumulation of Cd, but not other micronutrients in the grain. Our results indicated that OsHMA3 from the low Cd-accumulating cultivar limits translocation of Cd from the roots to the above-ground tissues by selectively sequestrating Cd into the root vacuoles.

    DOI: 10.1073/pnas.1005396107

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  • Gene limiting cadmium accumulation in rice 査読

    Daisei Ueno, Naoki Yamaji, Izumi Kono, Chao Feng Huang, Tsuyu Ando, Masahiro Yano, Jian Feng Ma

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   107 ( 38 )   16500 - 16505   2010年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Intake of toxic cadmium (Cd) from rice caused Itai-itai disease in the past and it is still a threat for human health. Therefore, control of the accumulation of Cd from soil is an important food-safety issue, but the molecular mechanism for the control is unknown. Herein, we report a gene (OsHMA3) responsible for low Cd accumulation in rice that was isolated from a mapping population derived from a cross between a high and low Cd-accumulating cultivar. The gene encodes a transporter belonging to the P(1B)-type ATPase family, but shares low similarity with other members. Heterologous expression in yeast showed that the transporter from the low-Cd cultivar is functional, but the transporter from the high-Cd cultivar had lost its function, probably because of the single amino acid mutation. The transporter is mainly expressed in the tonoplast of root cells at a similar level in both the low and high Cd-accumulating cultivars. Overexpression of the functional gene from the low Cd-accumulating cultivar selectively decreased accumulation of Cd, but not other micronutrients in the grain. Our results indicated that OsHMA3 from the low Cd-accumulating cultivar limits translocation of Cd from the roots to the above-ground tissues by selectively sequestrating Cd into the root vacuoles.

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  • Knockout of a Bacterial-Type ATP-Binding Cassette Transporter Gene, AtSTAR1, Results in Increased Aluminum Sensitivity in Arabidopsis 査読

    Chao-Feng Huang, Naoki Yamaji, Jian Feng Ma

    PLANT PHYSIOLOGY   153 ( 4 )   1669 - 1677   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    ATP-binding cassette (ABC) transporters represent a large family in plants, but the functions of most of these transporters are unknown. Here we report a gene, AtSTAR1, only encoding an ATP-binding domain of a bacterial-type ABC transporter in Arabidopsis (Arabidopsis thaliana). AtSTAR1 is an ortholog of rice (Oryza sativa) OsSTAR1, which has been implicated in aluminum (Al) tolerance. Knockout of AtSTAR1 resulted in increased sensitivity to Al and earlier flowering. Unlike OsSTAR1, AtSTAR1 was expressed in both the roots and shoots and its expression was not induced by Al or other stresses. Investigation of tissue-specific localization of AtSTAR1 through beta-glucuronidase fusion revealed that AtSTAR1 was predominantly expressed at outer cell layers of root tips and developing leaves, whose localization is also different from those of OsSTAR1. However, introduction of OsSTAR1 into atstar1 mutant rescued the sensitivity of atstar1 to Al, indicating that AtSTAR1 has a similar function as OsSTAR1. Furthermore, we found that AtSTAR1 may interact with ALS3, a transmembrane-binding domain in Arabidopsis to form a complex because introduction of OsSTAR1, a functional substitute of AtSTAR1, into als3 mutant resulted in the loss of OsSTAR1 protein. All these findings indicate that AtSTAR1 is involved in the basic detoxification of Al in Arabidopsis.

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  • Involvement of Silicon Influx Transporter OsNIP2;1 in Selenite Uptake in Rice 査読

    Xue Qiang Zhao, Namiki Mitani, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    PLANT PHYSIOLOGY   153 ( 4 )   1871 - 1877   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) as a staple food, provides a major source of dietary selenium (Se) for humans, which essentially requires Se, however, the molecular mechanism for Se uptake is still poorly understood. Herein, we show evidence that the uptake of selenite, a main bioavailable form of Se in paddy soils, is mediated by a silicon (Si) influx transporter Lsi1 (OsNIP2;1) in rice. Defect of OsNIP2;1 resulted in a significant decrease in the Se concentration of the shoots and xylem sap when selenite was given. However, there was no difference in the Se concentration between the wild-type rice and mutant of OsNIP2;1 when selenate was supplied. A short-term uptake experiment showed that selenite uptake greatly increased with decreasing pH in the external solution. Si as silicic acid did not inhibit the Se uptake from selenite in both rice and yeast (Saccharomyces cerevisiae) at low pHs. Expression of OsNIP2;1 in yeast enhanced the selenite uptake at pH 3.5 and 5.5 but not at pH 7.5. On the other hand, defect of Si efflux transporter Lsi2 did not affect the uptake of Se either from selenite or selenate. Taken together, our results indicate that Si influx transporter OsNIP2;1 is permeable to selenite.

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  • Involvement of Silicon Influx Transporter OsNIP2;1 in Selenite Uptake in Rice 査読

    Xue Qiang Zhao, Namiki Mitani, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

    PLANT PHYSIOLOGY   153 ( 4 )   1871 - 1877   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) as a staple food, provides a major source of dietary selenium (Se) for humans, which essentially requires Se, however, the molecular mechanism for Se uptake is still poorly understood. Herein, we show evidence that the uptake of selenite, a main bioavailable form of Se in paddy soils, is mediated by a silicon (Si) influx transporter Lsi1 (OsNIP2;1) in rice. Defect of OsNIP2;1 resulted in a significant decrease in the Se concentration of the shoots and xylem sap when selenite was given. However, there was no difference in the Se concentration between the wild-type rice and mutant of OsNIP2;1 when selenate was supplied. A short-term uptake experiment showed that selenite uptake greatly increased with decreasing pH in the external solution. Si as silicic acid did not inhibit the Se uptake from selenite in both rice and yeast (Saccharomyces cerevisiae) at low pHs. Expression of OsNIP2;1 in yeast enhanced the selenite uptake at pH 3.5 and 5.5 but not at pH 7.5. On the other hand, defect of Si efflux transporter Lsi2 did not affect the uptake of Se either from selenite or selenate. Taken together, our results indicate that Si influx transporter OsNIP2;1 is permeable to selenite.

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  • Knockout of a Bacterial-Type ATP-Binding Cassette Transporter Gene, AtSTAR1, Results in Increased Aluminum Sensitivity in Arabidopsis 査読

    Chao-Feng Huang, Naoki Yamaji, Jian Feng Ma

    PLANT PHYSIOLOGY   153 ( 4 )   1669 - 1677   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    ATP-binding cassette (ABC) transporters represent a large family in plants, but the functions of most of these transporters are unknown. Here we report a gene, AtSTAR1, only encoding an ATP-binding domain of a bacterial-type ABC transporter in Arabidopsis (Arabidopsis thaliana). AtSTAR1 is an ortholog of rice (Oryza sativa) OsSTAR1, which has been implicated in aluminum (Al) tolerance. Knockout of AtSTAR1 resulted in increased sensitivity to Al and earlier flowering. Unlike OsSTAR1, AtSTAR1 was expressed in both the roots and shoots and its expression was not induced by Al or other stresses. Investigation of tissue-specific localization of AtSTAR1 through beta-glucuronidase fusion revealed that AtSTAR1 was predominantly expressed at outer cell layers of root tips and developing leaves, whose localization is also different from those of OsSTAR1. However, introduction of OsSTAR1 into atstar1 mutant rescued the sensitivity of atstar1 to Al, indicating that AtSTAR1 has a similar function as OsSTAR1. Furthermore, we found that AtSTAR1 may interact with ALS3, a transmembrane-binding domain in Arabidopsis to form a complex because introduction of OsSTAR1, a functional substitute of AtSTAR1, into als3 mutant resulted in the loss of OsSTAR1 protein. All these findings indicate that AtSTAR1 is involved in the basic detoxification of Al in Arabidopsis.

    DOI: 10.1104/pp.110.155028

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  • A specific transporter for iron(III)-phytosiderophore in barley roots (vol 46, pg 563, 2006) 査読

    Y. Murata, J. F. Ma, N. Yamaji, D. Ueno, K. Nomoto, T. Iwashita

    PLANT JOURNAL   61 ( 1 )   188 - 188   2010年1月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    DOI: 10.1111/j.1365-313X.2009.04036.x

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  • A specific transporter for iron(III)-phytosiderophore in barley roots (vol 46, pg 563, 2006) 査読

    Y. Murata, J. F. Ma, N. Yamaji, D. Ueno, K. Nomoto, T. Iwashita

    PLANT JOURNAL   61 ( 1 )   188 - 188   2010年1月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    DOI: 10.1111/j.1365-313X.2009.04036.x

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  • The role of the rice aquaporin Lsi1 in arsenite efflux from roots 査読

    Fang-Jie Zhao, Yukiko Ago, Namiki Mitani, Ren-Ying Li, Yu-Hong Su, Naoki Yamaji, Steve P. McGrath, Jian Feng Ma

    NEW PHYTOLOGIST   186 ( 2 )   392 - 399   2010年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    When supplied with arsenate (As(V)), plant roots extrude a substantial amount of arsenite (As(III)) to the external medium through as yet unidentified pathways. The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots. Whether Lsi1 also mediates arsenite efflux was investigated.
    Expression of Lsi1 in Xenopus laevis oocytes enhanced arsenite efflux, indicating that Lsi1 facilitates arsenite transport bidirectionally.
    Arsenite was the predominant arsenic species in arsenate-exposed rice plants. During 24-h exposure to 5 mu M arsenate, rice roots extruded arsenite to the external medium rapidly, accounting for 60-90% of the arsenate uptake. A rice mutant defective in Lsi1 (lsi1) extruded significantly less arsenite than the wild-type rice and, as a result, accumulated more arsenite in the roots. By contrast, Lsi2 mutation had little effect on arsenite efflux to the external medium.
    We conclude that Lsi1 plays a role in arsenite efflux in rice roots exposed to arsenate. However, this pathway accounts for only 15-20% of the total efflux, suggesting the existence of other efflux transporters.

    DOI: 10.1111/j.1469-8137.2010.03192.x

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  • Isolation and characterisation of two MATE genes in rye 査読

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    FUNCTIONAL PLANT BIOLOGY   37 ( 4 )   296 - 303   2010年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CSIRO PUBLISHING  

    Multidrug and toxic compound extrusion (MATE) proteins are widely present in bacteria, fungi, plants and mammals. Recent studies have showed that a group of plant MATE genes encodes citrate transporter, which are involved in the detoxification of aluminium or translocation of iron from the roots to the shoots. In this study, we isolated two homologous genes (ScFRDL1 and ScFRDL2) from this family in rye (Secale cereale L.). ScFRDL1 shared 94.2% identity with HvAACT1, an Al-activated citrate transporter in barley (Hordeum vulgare L.) and ScFRDL2 shared 80.6% identity with OsFRDL2, a putative Al-responsive protein in rice (Oryza sativa L.). Both genes were mainly expressed in the roots, however, they showed different expression patterns. Expression of ScFRDL1 was unaffected by Al treatment, but up-regulated by Fe-deficiency treatment. In contrast, expression of ScFRDL2 was greatly induced by Al but not by Fe deficiency. The Al-induced up-regulation of ScFRDL2 was found in both the root tips and basal roots. Furthermore, the expression pattern of ScFRDL2 was consistent with citrate secretion pattern. Immunostaining showed that ScFRDL1 was localised at all cells in the root tips and central cylinder and endodermis in the basal root. Taken together, our results suggest that ScFRDL1 was involved in efflux of citrate into the xylem for Fe translocation from the roots to the shoots, while ScFRDL2 was involved in Al-activated citrate secretion in rye.

    DOI: 10.1071/FP09265

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  • Isolation and characterisation of two MATE genes in rye 査読

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma

    FUNCTIONAL PLANT BIOLOGY   37 ( 4 )   296 - 303   2010年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CSIRO PUBLISHING  

    Multidrug and toxic compound extrusion (MATE) proteins are widely present in bacteria, fungi, plants and mammals. Recent studies have showed that a group of plant MATE genes encodes citrate transporter, which are involved in the detoxification of aluminium or translocation of iron from the roots to the shoots. In this study, we isolated two homologous genes (ScFRDL1 and ScFRDL2) from this family in rye (Secale cereale L.). ScFRDL1 shared 94.2% identity with HvAACT1, an Al-activated citrate transporter in barley (Hordeum vulgare L.) and ScFRDL2 shared 80.6% identity with OsFRDL2, a putative Al-responsive protein in rice (Oryza sativa L.). Both genes were mainly expressed in the roots, however, they showed different expression patterns. Expression of ScFRDL1 was unaffected by Al treatment, but up-regulated by Fe-deficiency treatment. In contrast, expression of ScFRDL2 was greatly induced by Al but not by Fe deficiency. The Al-induced up-regulation of ScFRDL2 was found in both the root tips and basal roots. Furthermore, the expression pattern of ScFRDL2 was consistent with citrate secretion pattern. Immunostaining showed that ScFRDL1 was localised at all cells in the root tips and central cylinder and endodermis in the basal root. Taken together, our results suggest that ScFRDL1 was involved in efflux of citrate into the xylem for Fe translocation from the roots to the shoots, while ScFRDL2 was involved in Al-activated citrate secretion in rye.

    DOI: 10.1071/FP09265

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  • The role of the rice aquaporin Lsi1 in arsenite efflux from roots 査読

    Fang-Jie Zhao, Yukiko Ago, Namiki Mitani, Ren-Ying Li, Yu-Hong Su, Naoki Yamaji, Steve P. McGrath, Jian Feng Ma

    NEW PHYTOLOGIST   186 ( 2 )   392 - 399   2010年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    When supplied with arsenate (As(V)), plant roots extrude a substantial amount of arsenite (As(III)) to the external medium through as yet unidentified pathways. The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots. Whether Lsi1 also mediates arsenite efflux was investigated.
    Expression of Lsi1 in Xenopus laevis oocytes enhanced arsenite efflux, indicating that Lsi1 facilitates arsenite transport bidirectionally.
    Arsenite was the predominant arsenic species in arsenate-exposed rice plants. During 24-h exposure to 5 mu M arsenate, rice roots extruded arsenite to the external medium rapidly, accounting for 60-90% of the arsenate uptake. A rice mutant defective in Lsi1 (lsi1) extruded significantly less arsenite than the wild-type rice and, as a result, accumulated more arsenite in the roots. By contrast, Lsi2 mutation had little effect on arsenite efflux to the external medium.
    We conclude that Lsi1 plays a role in arsenite efflux in rice roots exposed to arsenate. However, this pathway accounts for only 15-20% of the total efflux, suggesting the existence of other efflux transporters.

    DOI: 10.1111/j.1469-8137.2010.03192.x

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  • Isolation and characterisation of two MATE genes in rye 査読

    Naoki Yamaji

    Functional Plant Biology   37 ( 4 )   2010年

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    掲載種別:研究論文(学術雑誌)  

    Kengo Yokosho, Naoki Yamaji, Jian Feng Ma, 2010, &#039;Isolation and characterisation of two MATE genes in rye&#039;, &lt;i&gt;Functional Plant Biology&lt;/i&gt;, vol. 37, no. 4, p. 296

    DOI: 10.1071/fp09265

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  • The role of the rice aquaporin Lsi1 in arsenite efflux from roots 査読

    Fang-Jie Zhao, Yukiko Ago, Namiki Mitani, Ren-Ying Li, Yu-Hong Su, Naoki Yamaji, Steve P. McGrath, Jian Feng Ma

    NEW PHYTOLOGIST   186 ( 2 )   392 - 399   2010年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    When supplied with arsenate (As(V)), plant roots extrude a substantial amount of arsenite (As(III)) to the external medium through as yet unidentified pathways. The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots. Whether Lsi1 also mediates arsenite efflux was investigated.
    Expression of Lsi1 in Xenopus laevis oocytes enhanced arsenite efflux, indicating that Lsi1 facilitates arsenite transport bidirectionally.
    Arsenite was the predominant arsenic species in arsenate-exposed rice plants. During 24-h exposure to 5 mu M arsenate, rice roots extruded arsenite to the external medium rapidly, accounting for 60-90% of the arsenate uptake. A rice mutant defective in Lsi1 (lsi1) extruded significantly less arsenite than the wild-type rice and, as a result, accumulated more arsenite in the roots. By contrast, Lsi2 mutation had little effect on arsenite efflux to the external medium.
    We conclude that Lsi1 plays a role in arsenite efflux in rice roots exposed to arsenate. However, this pathway accounts for only 15-20% of the total efflux, suggesting the existence of other efflux transporters.

    DOI: 10.1111/j.1469-8137.2010.03192.x

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  • A Zinc Finger Transcription Factor ART1 Regulates Multiple Genes Implicated in Aluminum Tolerance in Rice 査読

    Naoki Yamaji, Chao Feng Huang, Sakiko Nagao, Masahiro Yano, Yutaka Sato, Yoshiaki Nagamura, Jian Feng Ma

    PLANT CELL   21 ( 10 )   3339 - 3349   2009年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Aluminum (Al) toxicity is the major limiting factor of crop production on acid soils, but some plant species have evolved ways of detoxifying Al. Here, we report a C2H2-type zinc finger transcription factor ART1 (for Al resistance transcription factor 1), which specifically regulates the expression of genes related to Al tolerance in rice (Oryza sativa). ART1 is constitutively expressed in the root, and the expression level is not affected by Al treatment. ART1 is localized in the nucleus of all root cells. A yeast one-hybrid assay showed that ART1 has a transcriptional activation potential and interacts with the promoter region of STAR1, an important factor in rice Al tolerance. Microarray analysis revealed 31 downstream transcripts regulated by ART1, including STAR1 and 2 and a couple of homologs of Al tolerance genes in other plants. Some of these genes were implicated in both internal and external detoxification of Al at different cellular levels. Our findings shed light on comprehensively understanding how plants detoxify aluminum to survive in an acidic environment.

    DOI: 10.1105/tpc.109.070771

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  • A Transporter at the Node Responsible for Intervascular Transfer of Silicon in Rice 査読

    Naoki Yamaji, Jian Feng Ma

    PLANT CELL   21 ( 9 )   2878 - 2883   2009年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    The concentration of essential mineral nutrients in the edible portion of plants such as grains may affect the nutritional value of these foods, while concentrations of toxic minerals in the plant are matter of food safety. Minerals taken up by the roots from soils are normally redirected at plant nodes before they are finally transported into developing seeds. However, the molecular mechanisms involved in this process have not been identified so far. Herein, we report on a transporter (Lsi6) responsible for the redirection of a plant nutrient at the node. Lsi6 is a silicon transporter in rice (Oryza sativa), and its expression in node I below the panicles is greatly enhanced when the panicle is completely emerged. Lsi6 is mainly localized at the xylem transfer cells located at the outer boundary region of the enlarged large vascular bundles in node I. Knockout of Lsi6 decreased Si accumulation in the panicles but increased Si accumulation in the flag leaf. These results suggest that Lsi6 is a transporter involved in intervascular transfer (i.e., transfer of silicon from the large vascular bundles coming from the roots to the diffuse vascular bundles connected to the panicles). These findings will be useful for selectively enhancing the accumulation of essential nutrients and reducing toxic minerals in the edible portion of cereals.

    DOI: 10.1105/tpc.109.069831

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  • Further characterization of ferric-phytosiderophore transporters ZmYS1 and HvYS1 in maize and barley 査読

    Daisei Ueno, Naoki Yamaji, Jian Feng Ma

    JOURNAL OF EXPERIMENTAL BOTANY   60 ( 12 )   3513 - 3520   2009年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Roots of some gramineous plants secrete phytosiderophores in response to iron deficiency and take up Fe as a ferric-phytosiderophore complex through the transporter YS1 (Yellow Stripe 1). Here, this transporter in maize (ZmYS1) and barley (HvYS1) was further characterized and compared in terms of expression pattern, diurnal change, and tissue-type specificity of localization. The expression of HvYS1 was specifically induced by Fe deficiency only in barley roots, and increased with the progress of Fe deficiency, whereas ZmYS1 was expressed in maize in the leaf blades and sheaths, crown, and seminal roots, but not in the hypocotyl. HvYS1 expression was not induced by any other metal deficiency. Furthermore, in maize leaf blades, the expression was higher in the young leaf blades showing severe chlorosis than in the old leaf blades showing no chlorosis. The expression of HvYS1 showed a distinct diurnal rhythm, reaching a maximum before the onset of phytosiderophore secretion. In contrast, ZmYS1 did not show such a rhythm in expression. Immunostaining showed that ZmYS1 was localized in the epidermal cells of both crown and lateral roots, with a polar localization at the distal side of the epidermal cells. In maize leaves, ZmYS1 was localized in mesophyll cells, but not epidermal cells. These differences in gene expression pattern and tissue-type specificity of localization suggest that HvYS1 is only involved in primary Fe acquisition by barley roots, whereas ZmYS1 is involved in both primary Fe acquisition and intracellular transport of iron and other metals in maize.

    DOI: 10.1093/jxb/erp191

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  • Identification and Characterization of Maize and Barley Lsi2-Like Silicon Efflux Transporters Reveals a Distinct Silicon Uptake System from That in Rice 査読

    Namiki Mitani, Yukako Chiba, Naoki Yamaji, Jian Feng Ma

    PLANT CELL   21 ( 7 )   2133 - 2142   2009年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Silicon (Si) uptake has been extensively examined in rice (Oryza sativa), but it is poorly understood in other gramineous crops. We identified Low Silicon Rice 2 (Lsi2)-like Si efflux transporters from two important gramineous crops: maize (Zea mays) and barley (Hordeum vulgare). Both maize and barley Lsi2 expressed in Xenopus laevis oocytes showed Si efflux transport activity. Furthermore, barley Lsi2 was able to recover Si uptake in a rice mutant defective in Si efflux. Maize and barley Lsi2 were only expressed in the roots. Expression of maize and barley Lsi2 was downregulated in response to exogenously applied Si. Moreover, there was a significant positive correlation between the ability of roots to absorb Si and the expression levels of Lsi2 in eight barley cultivars, suggesting that Lsi2 is a key Si transporter in barley. Immunostaining showed that maize and barley Lsi2 localized only at the endodermis, with no polarity. Protein gel blot analysis indicated that maize and barley Lsi2 localized on the plasma membrane. The unique features of maize and barley Si influx and efflux transporters, including their cell-type specificity and the lack of polarity of their localization in Lsi2, indicate that these crops have a different Si uptake system from that in rice.

    DOI: 10.1105/tpc.109.067884

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  • A Rice Mutant Sensitive to Al Toxicity is Defective in the Specification of Root Outer Cell Layers 査読

    Chao-Feng Huang, Naoki Yamaji, Minoru Nishimura, Shigeyuki Tajima, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   50 ( 5 )   976 - 985   2009年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Outer cell layers of rice roots, which comprise epidermis, exodermis and sclerenchyma, have been proposed to protect the roots from various stresses in soil. Here, we report a mutant which is defective in the specification of outer cell layers, and examined the role of these layers in Al and other metal resistance. Morphological and histochemical observations revealed that the mutant isolated based on Al sensitivity frequently showed a disordered pattern of periclinal cell division in the epidermal layers at a region close to the root apical meristem. The lateral root caps in the mutant became difficult to peel off from the epidermis, and epidermal cells became smaller and irregular with far fewer root hairs. Furthermore, some exodermal cells were transformed into additional sclerenchyma cells. However, there was no difference in the inner cell layers between the wild-type rice and the mutant. The mutant showed similar root growth to the wild-type rice in the absence of Al, but greater inhibition of root elongation by Al was found in the mutant. Morin staining showed that Al penetrated into the inner cortical cells in the mutant. Furthermore, the mutant was also sensitive to other metals including Cd and La. Taken together, our results indicate that root outer cell layers protect the roots against the toxicity of Al and other metals by preventing metal penetration into the inner cells. Genetic analysis showed that the mutant phenotypes were controlled by a single recessive gene, which was located on the short arm of rice chromosome 2.

    DOI: 10.1093/pcp/pcp050

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  • HvLsi1 is a silicon influx transporter in barley 査読

    Yukako Chiba, Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    PLANT JOURNAL   57 ( 5 )   810 - 818   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Most plants accumulate silicon in their bodies, and this is thought to be important for resistance against biotic and abiotic stresses; however, the molecular mechanisms for Si uptake and accumulation are poorly understood. Here, we describe an Si influx transporter, HvLsi1, in barley. This protein is homologous to rice influx transporter OsLsi1 with 81% identity, and belongs to a Nod26-like major intrinsic protein sub-family of aquaporins. Heterologous expression in both Xenopus laevis oocytes and a rice mutant defective in Si uptake showed that HvLsi1 has transport activity for silicic acid. Expression of HvLsi1 was detected specifically in the basal root, and the expression level was not affected by Si supply. There was a weak correlation between Si uptake and the expression level of HvLsi1 in eight cultivars tested. In the seminal roots, HvLsi1 is localized on the plasma membrane on the distal side of epidermal and cortical cells. HvLsi1 is also located in lateral roots on the plasma membrane of hypodermal cells. These cell-type specificity of localization and expression patterns of HvLsi1 are different from those of OsLsi1. These observations indicate that HvLsi1 is a silicon influx transporter that is involved in radial transport of Si through the epidermal and cortical layers of the basal roots of barley.

    DOI: 10.1111/j.1365-313X.2008.03728.x

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  • HvLsi1 is a silicon influx transporter in barley 査読

    Yukako Chiba, Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    PLANT JOURNAL   57 ( 5 )   810 - 818   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Most plants accumulate silicon in their bodies, and this is thought to be important for resistance against biotic and abiotic stresses; however, the molecular mechanisms for Si uptake and accumulation are poorly understood. Here, we describe an Si influx transporter, HvLsi1, in barley. This protein is homologous to rice influx transporter OsLsi1 with 81% identity, and belongs to a Nod26-like major intrinsic protein sub-family of aquaporins. Heterologous expression in both Xenopus laevis oocytes and a rice mutant defective in Si uptake showed that HvLsi1 has transport activity for silicic acid. Expression of HvLsi1 was detected specifically in the basal root, and the expression level was not affected by Si supply. There was a weak correlation between Si uptake and the expression level of HvLsi1 in eight cultivars tested. In the seminal roots, HvLsi1 is localized on the plasma membrane on the distal side of epidermal and cortical cells. HvLsi1 is also located in lateral roots on the plasma membrane of hypodermal cells. These cell-type specificity of localization and expression patterns of HvLsi1 are different from those of OsLsi1. These observations indicate that HvLsi1 is a silicon influx transporter that is involved in radial transport of Si through the epidermal and cortical layers of the basal roots of barley.

    DOI: 10.1111/j.1365-313X.2008.03728.x

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  • A Bacterial-Type ABC Transporter Is Involved in Aluminum Tolerance in Rice 査読

    Chao Feng Huang, Naoki Yamaji, Namiki Mitani, Masahiro Yano, Yoshiaki Nagamura, Jian Feng Ma

    PLANT CELL   21 ( 2 )   655 - 667   2009年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Aluminum (Al) toxicity is a major factor limiting crop production in acidic soil, but the molecular mechanisms of Al tolerance are poorly understood. Here, we report that two genes, STAR1 (for sensitive to Al rhizotoxicity1) and STAR2, are responsible for Al tolerance in rice. STAR1 encodes a nucleotide binding domain, while STAR2 encodes a transmembrane domain, of a bacterial-type ATP binding cassette (ABC) transporter. Disruption of either gene resulted in hypersensitivity to aluminum toxicity. Both STAR1 and STAR2 are expressed mainly in the roots and are specifically induced by Al exposure. Expression in onion epidermal cells, rice protoplasts, and yeast showed that STAR1 interacts with STAR2 to form a complex that localizes to the vesicle membranes of all root cells, except for those in the epidermal layer of the mature zone. When expressed together in Xenopus laevis oocytes, STAR1/2 shows efflux transport activity specific for UDP-glucose. Furthermore, addition of exogenous UDP-glucose rescued root growth in the star1 mutant exposed to Al. These results indicate that STAR1 and STAR2 form a complex that functions as an ABC transporter, which is required for detoxification of Al in rice. The ABC transporter transports UDP-glucose, which may be used to modify the cell wall.

    DOI: 10.1105/tpc.108.064543

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  • OsFRDL1 Is a Citrate Transporter Required for Efficient Translocation of Iron in Rice 査読

    Kengo Yokosho, Naoki Yamaji, Daisei Ueno, Namiki Mitani, Jian Feng Ma

    PLANT PHYSIOLOGY   149 ( 1 )   297 - 305   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Multidrug and toxic compound extrusion (MATE) transporters represent a large family in plants, but their functions are poorly understood. Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1), the closest homolog of barley (Hordeum vulgare) HvAACT1 (aluminum [Al]-activated citrate transporter 1), in terms of metal stress (iron [Fe] deficiency and Al toxicity). This gene was mainly expressed in the roots and the expression level was not affected by either Fe deficiency or Al toxicity. Knockout of this gene resulted in leaf chlorosis, lower leaf Fe concentration, higher accumulation of zinc and manganese concentration in the leaves, and precipitation of Fe in the root&apos;s stele. The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line compared to the wild-type rice. Heterologous expression of OsFRDL1 in Xenopus oocytes showed transport activity for citrate. Immunostaining showed that OsFRDL1 was localized at the pericycle cells of the roots. On the other hand, there was no difference in the Al-induced secretion of citrate from the roots between the knockout line and the wild-type rice. Taken together, our results indicate that OsFRDL1 is a citrate transporter localized at the pericycle cells, which is necessary for efficient translocation of Fe to the shoot as a Fe-citrate complex.

    DOI: 10.1104/pp.108.128132

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  • Identification of Maize Silicon Influx Transporters 査読

    Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   50 ( 1 )   5 - 12   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Maize (Zea mays L.) shows a high accumulation of silicon (Si), but transporters involved in the uptake and distribution have not been identified. In the present study, we isolated two genes (ZmLsi1 and ZmLsi6), which are homologous to rice influx Si transporter OsLsi1. Heterologous expression in Xenopus laevis oocytes showed that both ZmLsi1 and ZmLsi6 are permeable to silicic acid. ZmLsi1 was mainly expressed in the roots. By contrast, ZmLsi6 was expressed more in the leaf sheaths and blades. Different from OsLsi1, the expression level of both ZmLsi1 and ZmLsi6 was unaffected by Si supply. Immunostaining showed that ZmLsi1 was localized on the plasma membrane of the distal side of root epidermal and hypodermal cells in the seminal and crown roots, and also in cortex cells in lateral roots. In the shoots, ZmLsi6 was found in the xylem parenchyma cells that are adjacent to the vessels in both leaf sheaths and leaf blades. ZmLsi6 in the leaf sheaths and blades also exhibited polar localization on the side facing towards the vessel. Taken together, it can be concluded that ZmLsi1 is an influx transporter of Si, which is responsible for the transport of Si from the external solution to the root cells and that ZmLsi6 mainly functions as a Si transporter for xylem unloading.

    DOI: 10.1093/pcp/pcn110

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  • Specific transporter for iron(III)-phytosiderophore complex involved in iron uptake by barley roots 査読

    Yoshiko Murata, Emiko Harada, Kenji Sugase, Kosuke Namba, Manabu Horikawa, Jian Feng Ma, Naoki Yamaji, Daisei Ueno, Kyosuke Nomoto, Takashi Iwashita, Shoichi Kusumoto

    PURE AND APPLIED CHEMISTRY   80 ( 12 )   2689 - 2697   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT UNION PURE APPLIED CHEMISTRY  

    Iron (Fe) is an essential element for plant growth. Gramineous plants have generally developed a distinct strategy to efficiently acquire insoluble Fe, which is characterized by the synthesis and secretion of an Fe-chelating substance, phytosiderophore (PS) such as mugineic acid (MA), and by a specific uptake system for Fe(III)-PS complexes. In a previous study, we identified a gene specifically encoding an Fe(III)-PS transporter (HvYS1) in barley. This gene as well as the encoded protein is specifically expressed in the epidermal cells of the roots, and gene expression is greatly enhanced under Fe-deficient conditions. The localization and substrate specificity of HvYS1 indicate that it is a Fe(III)-PS specific transporter in barley roots. In contrast, ZmYS1, which has been reported as ail Fe-PS transporter from maize, possesses broad substrate specificity despite a high homology with HvYS1. By assessing the transport activity of a series of HvYS1-ZmYS1 chimeras, we revealed that the outer membrane loop between the 6(th) and 7(th) transmembrane regions is essential for the substrate specificity. We also achieved an efficient short-step synthesis of MA and 2&apos;-deoxymugineic acid (DMA). Our new synthetic method enabled us to use them in a large quantity for biological studies.

    DOI: 10.1351/pac200880122689

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  • Functions and transport of silicon in plants 査読

    J. F. Ma, N. Yamaji

    CELLULAR AND MOLECULAR LIFE SCIENCES   65 ( 19 )   3049 - 3057   2008年10月

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    記述言語:英語   出版者・発行元:BIRKHAUSER VERLAG AG  

    Silicon exerts beneficial effects on plant growth and production by alleviating both biotic and abiotic stresses including diseases, pests, lodging, drought, and nutrient imbalance. Recently, two genes (Lsi1 and Lsi2) encoding Si transporters have been identified from rice. Lsi1 (low silicon 1) belongs to a Nod26-like major intrinsic protein subfamily in aquaporin, while Lsi2 encodes a putative anion transporter. Lsi1 is localized on the distal side of both exodermis and endodermis in rice roots, while Lsi2 is localized on the proximal side of the same cells. Lsi1 shows influx transport activity for Si, while Lsi2 shows efflux transport activity. Therefore, Lsi1 is responsible for transport of Si from the external solution to the root cells, whereas Lsi2 is an efflux transporter responsible for the transport of Si from the root cells to the apoplast. Coupling of Lsi1 with Lsi2 is required for efficient uptake of Si in rice.

    DOI: 10.1007/s00018-008-7580-x

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  • Characterization of substrate specificity of a rice silicon transporter, Lsi1 査読

    Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY   456 ( 4 )   679 - 686   2008年7月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Lsi1 (OsNIP2;1) is the first silicon (silicic acid) transporter identified in plant, which belongs to the nodulin 26-like intrinsic membrane protein (NIP) subfamily. In this study, we characterized the function of this transporter by using the Xenopus laevis oocyte expression system. The transport activity of Lsi1 for silicic acid was significantly inhibited by HgCl(2) but not by low temperature. Lsi1 also showed an efflux transport activity for silicic acid. The substrate specificity study showed that Lsi1 was able to transport urea and boric acid; however, the transport activity for silicic acid was not affected by the presence of equimolar urea and was decreased only slightly by boric acid. Furthermore, among the NIPs subgroup, OsNIP2;2 showed transport activity for silicic acid, whereas OsNIP1;1 and OsNIP3;1 did not. We propose that Lsi1 and its close homologues form a unique subgroup of NIP with a distinct ar/R selectivity filter, which is located in the narrowest region on the extra-membrane mouth and govern the substrate specificity of the pore.

    DOI: 10.1007/s00424-007-0408-y

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  • Transporters of arsenite in rice and their role in arsenic accumulation in rice grain 査読

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Xiao-Yan Xu, Yu-Hong Su, Steve P. McGrath, Fang-Jie Zhao

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   105 ( 29 )   9931 - 9935   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Arsenic poisoning affects millions of people worldwide. Human arsenic intake from rice consumption can be substantial because rice is particularly efficient in assimilating arsenic from paddy soils, although the mechanism has not been elucidated. Here we report that two different types of transporters mediate transport of arsenite, the predominant form of arsenic in paddy soil, from the external medium to the xylem. Transporters belonging to the NIP subfamily of aquaporins in rice are permeable to arsenite but not to arsenate. Mutation in OsNIP2;1 (Lsi1, a silicon influx transporter) significantly decreases arsenite uptake. Furthermore, in the rice mutants defective in the silicon efflux transporter Lsi2, arsenite transport to the xylem and accumulation in shoots and grain decreased greatly. Mutation in Lsi2 had a much greater impact on arsenic accumulation in shoots and grain in field-grown rice than Lsi1. Arsenite transport in rice roots therefore shares the same highly efficient pathway as silicon, which explains why rice is efficient in arsenic accumulation. Our results provide insight into the uptake mechanism of arsenite in rice and strategies for reducing arsenic accumulation in grain for enhanced food safety.

    DOI: 10.1073/pnas.0802361105

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  • Characterization of substrate specificity of a rice silicon transporter, Lsi1 査読

    Namiki Mitani, Naoki Yamaji, Jian Feng Ma

    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY   456 ( 4 )   679 - 686   2008年7月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Lsi1 (OsNIP2;1) is the first silicon (silicic acid) transporter identified in plant, which belongs to the nodulin 26-like intrinsic membrane protein (NIP) subfamily. In this study, we characterized the function of this transporter by using the Xenopus laevis oocyte expression system. The transport activity of Lsi1 for silicic acid was significantly inhibited by HgCl(2) but not by low temperature. Lsi1 also showed an efflux transport activity for silicic acid. The substrate specificity study showed that Lsi1 was able to transport urea and boric acid; however, the transport activity for silicic acid was not affected by the presence of equimolar urea and was decreased only slightly by boric acid. Furthermore, among the NIPs subgroup, OsNIP2;2 showed transport activity for silicic acid, whereas OsNIP1;1 and OsNIP3;1 did not. We propose that Lsi1 and its close homologues form a unique subgroup of NIP with a distinct ar/R selectivity filter, which is located in the narrowest region on the extra-membrane mouth and govern the substrate specificity of the pore.

    DOI: 10.1007/s00424-007-0408-y

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  • A transporter regulating silicon distribution in rice shoots 査読

    Naoki Yamaji, Namiki Mitatni, Jian Feng Ma

    PLANT CELL   20 ( 5 )   1381 - 1389   2008年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) accumulates very high concentrations of silicon (Si) in the shoots, and the deposition of Si as amorphous silica helps plants to overcome biotic and abiotic stresses. Here, we describe a transporter, Lsi6, which is involved in the distribution of Si in the shoots. Lsi6 belongs to the nodulin-26 intrinsic protein III subgroup of aquaporins and is permeable to silicic acid. Lsi6 is expressed in the leaf sheath and leaf blades as well as in the root tips. Cellular localization studies revealed that Lsi6 is found in the xylem parenchyma cells of the leaf sheath and leaf blades. Moreover, Lsi6 showed polar localization at the side facing toward the vessel. Knockdown of Lsi6 did not affect the uptake of Si by the roots but resulted in disordered deposition of silica in the shoots and increased excretion of Si in the guttation fluid. These results indicate that Lsi6 is a transporter responsible for the transport of Si out of the xylem and subsequently affects the distribution of Si in the leaf.

    DOI: 10.1105/tpc.108.059311

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  • Genotypic difference in silicon uptake and expression of silicon transporter genes in rice 査読

    Jian Feng Ma, Naoki Yamaji, Kazunori Tamai, Namiki Mitani

    PLANT PHYSIOLOGY   145 ( 3 )   919 - 924   2007年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) is a highly silicon (Si)-accumulating species that shows genotypic differences in Si accumulation. We investigated the physiological and molecular mechanisms involved in the genotypic difference in Si uptake between the japonica var. Nipponbare and the indica var. Kasalath. Both the Si concentration in the shoot and the Si uptake per root dry weight were higher in Nipponbare than in Kasalath grown in either soil or nutrient solution. The Si uptake by a single root was also higher in Nipponbare than in Kasalath. A kinetics study showed that Nipponbare and Kasalath had a similar K-m value, whereas the V-max was higher in Nipponbare. The expression of two Si transporter genes (Low silicon rice 1 [Lsi1] and Lsi2) investigated using real-time reverse transcription polymerase chain reaction revealed higher expression of both genes in Nipponbare than in Kasalath. Immunostaining with Lsi1 and Lsi2 antibodies revealed a similar pattern of subcellular localization of these two Si transporters in both varieties; Lsi1 and Lsi2 were localized at the distal and proximal sides, respectively, of both exodermis and endodermis of the roots. These results revealed that the genotypic difference in the Si accumulation results from the difference in abundance of Si transporters in rice roots.

    DOI: 10.1104/pp.107.107599

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  • Silicon transporters in rice

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   52 ( 14 )   1849 - 1856   2007年11月

  • Shoot formation from root tip region: a developmental alteration by WUS in transgenic tobacco 査読

    Syeda Zinia Rashid, Naoki Yamaji, Masaharu Kyo

    PLANT CELL REPORTS   26 ( 9 )   1449 - 1455   2007年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    We examined the effect of ectopic expression of WUS on the morphology of tobacco seedlings and the segments in vitro. WUS was amplified from Arabidopsis cDNA and introduced into the tobacco genome under the transcriptional control of the beta-estradiol-inducible expression system. When 1-week-old transgenic seedlings were cultured in the presence of beta-estradiol, only the root tip region developed bulbous tissues followed by shoot formation and plant regeneration, suggesting its applicability for improving the strategy of micropropagation in recalcitrant species. Evident abnormality was not observed in the cotyledons, hypocotyl nor root except for the tip. However, ectopic WUS seemed to be functional in those parts through the observation of gene expression and the behavior of cultured segments. Small root segments with a root tip treated with beta-estradiol also showed bulbing but no shoots unless exogenous cytokinin was supplied. These findings suggest the existence of unknown factors regulating ectopic WUS function in the seedling.

    DOI: 10.1007/s00299-007-0342-7

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  • An aluminum-activated citrate transporter in barley. 査読

    Jun Furukawa, Naoki Yamaji, Hua Wang, Namiki Mitani, Yoshiko Murata, Kazuhiro Sato, Maki Katsuhara, Kazuyoshi Takeda, Jian Feng Ma

    Plant & cell physiology   48 ( 8 )   1081 - 91   2007年8月

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    記述言語:英語  

    Soluble ionic aluminum (Al) inhibits root growth and reduces crop production on acid soils. Al-resistant cultivars of barley (Hordeum vulgare L.) detoxify Al by secreting citrate from the roots, but the responsible gene has not been identified yet. Here, we identified a gene (HvAACT1) responsible for the Al-activated citrate secretion by fine mapping combined with microarray analysis, using an Al-resistant cultivar, Murasakimochi, and an Al-sensitive cultivar, Morex. This gene belongs to the multidrug and toxic compound extrusion (MATE) family and was constitutively expressed mainly in the roots of the Al-resistant barley cultivar. Heterologous expression of HvAACT1 in Xenopus oocytes showed efflux activity for (14)C-labeled citrate, but not for malate. Two-electrode voltage clamp analysis also showed transport activity of citrate in the HvAACT1-expressing oocytes in the presence of Al. Overexpression of this gene in tobacco enhanced citrate secretion and Al resistance compared with the wild-type plants. Transiently expressed green fluorescent protein-tagged HvAACT1 was localized at the plasma membrane of the onion epidermal cells, and immunostaining showed that HvAACT1 was localized in the epidermal cells of the barley root tips. A good correlation was found between the expression of HvAACT1 and citrate secretion in 10 barley cultivars differing in Al resistance. Taken together, our results demonstrate that HvAACT1 is an Al-activated citrate transporter responsible for Al resistance in barley.

    DOI: 10.1093/pcp/pcm091

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  • An efflux transporter of silicon in rice 査読

    Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Kazunori Tamai, Saeko Konishi, Toru Fujiwara, Maki Katsuhara, Masahiro Yano

    NATURE   448 ( 7150 )   209 - U12   2007年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Silicon is an important nutrient for the optimal growth and sustainable production of rice(1-4). Rice accumulates up to 10% silicon in the shoot, and this high accumulation is required to protect the plant from multiple abiotic and biotic stresses(1-5). A gene, Lsi1, that encodes a silicon influx transporter has been identified in rice(6). Here we describe a previously uncharacterized gene, low silicon rice 2 (Lsi2), which has no similarity to Lsi1. This gene is constitutively expressed in the roots. The protein encoded by this gene is localized, like Lsi1, on the plasma membrane of cells in both the exodermis and the endodermis, but in contrast to Lsi1, which is localized on the distal side, Lsi2 is localized on the proximal side of the same cells. Expression of Lsi2 in Xenopus oocytes did not result in influx transport activity for silicon, but preloading of the oocytes with silicon resulted in a release of silicon, indicating that Lsi2 is a silicon efflux transporter. The identification of this silicon transporter revealed a unique mechanism of nutrient transport in plants: having an influx transporter on one side and an efflux transporter on the other side of the cell to permit the effective transcellular transport of the nutrients.

    DOI: 10.1038/nature05964

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  • Sorghum roots are inefficient in uptake of EDTA-chelated lead 査読

    Yong Xu, Naoki Yamaji, Renfang Shen, Jian Feng Ma

    ANNALS OF BOTANY   99 ( 5 )   869 - 875   2007年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Background and Aims Ethylene diamine tetraacetic acid (EDTA)-assisted phytoremediation has been developed to clean up lead (Pb)-contaminated soil; however, the mechanism responsible for the uptake of EDTA-Pb complex is not well understood. In this study, the accumulation process of Pb from EDTA-Pb is characterized in comparison to ionic Pb [Pb(NO3)(2)] in sorghum (Sorghum bicolor).
    Methods Sorghum seedlings were exposed to a 0.5 mm CaCl2 (pH 5.0) solution containing 0, 1 mm Pb(NO3)(2) or EDTA-Pb complexes at a molar ratio of 1:0.5, 1:1, 1:2 and 1:4 (Pb:EDTA). The root elongation of sorghum at different ratios of Pb:EDTA was measured. Xylem sap was collected after the stem was severed at different times. The concentration of Pb in the shoots and roots were determined by an atomic absorption spectrometer. In addition, the roots were stained with Fluostain I for observation of the root structure.
    Key Results Lead accumulation in the shoots of the plants exposed to EDTA-Pb at 1:1 ratio was only one-fifth of that exposed to ionic Pb at the same concentration. Lead accumulation decreased when transpiration was suppressed. The concentration of Pb in the xylem sap from the EDTA-Pb-treated plants was about 1/25 000 of that in the external solution. Root elongation was severely inhibited by ionic Pb, but not by EDTA-Pb at a 1:1 ratio. Root staining showed that a physiological barrier was damaged in the roots exposed to ionic Pb, but not in the roots exposed to EDTA-Pb.
    Conclusions All these results suggest that sorghum roots are inefficient in uptake of EDTA-chelated Pb and that enhanced Pb accumulation from ionic Pb was attributed to the damaged structure of the roots.

    DOI: 10.1093/aob/mcm038

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  • Spatial distribution and temporal variation of the rice silicon transporter Lsi1 査読

    Naoki Yamaji, Jian Feng Ma

    PLANT PHYSIOLOGY   143 ( 3 )   1306 - 1313   2007年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Rice (Oryza sativa) is a typical silicon (Si) accumulator and requires a large amount of Si for high-yield production. Recently, a gene (Low silicon rice1 [Lsi1]) encoding a Si transporter was identified in rice roots. Here, we characterized Lsi1 in terms of spatial distribution and temporal variation using both physiological and molecular approaches. Results from a multicompartment transport box experiment showed that the major site for Si uptake was located at the basal zone (&gt; 10 mm from the root tip) of the roots rather than at the root tips (&lt; 10 mm from the root tip). Consistent with the Si uptake pattern, Lsi1 expression and distribution of the Lsi1 protein were found only in the basal zone of roots. In the basal zones of the seminal, crown, and lateral roots, the Lsi1 protein showed a polar localization at the distal side of both the exodermis and endodermis, where the Casparian bands are formed. This indicates that Lsi1 is required for the transport of Si through the cells of the exodermis and endodermis. Expression of Lsi1 displayed a distinct diurnal pattern. Furthermore, expression was transiently enhanced around the heading stage, which coincides with a high Si requirement during this growth stage. Expression was down-regulated by dehydration stress and abscisic acid, suggesting that expression of Lsi1 may be regulated by abscisic acid.

    DOI: 10.1104/pp.106.093005

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  • Gene expression and functional analysis of a candidate gene related to Al-activated citrate transporter in barley 査読

    Jun Furukawa, Naoki Yamaji, Hua Wang, Maki Katsuhara, Kazuhiro Sato, Kazuyoshi Takeda, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   48   S44 - S44   2007年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

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  • Functional analysis of a rice Al-tolerant gene Als1 査読

    Chaofeng Huang, Naoki Yamaji, Masahiro Yano, Jianfeng Ma

    PLANT AND CELL PHYSIOLOGY   48   S45 - S45   2007年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    DOI: 10.14841/jspp.2007.0.113.0

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  • Further characterization of an Fe-phytosiderophore transporter gene ZmYS 1 in maize 査読

    Daisei Ueno, Naoki Yamaji, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   48   S29 - S29   2007年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

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  • Functional analysis of a rice Si transporter homolog Lsi6. 査読

    Naoki Yamaji, Namiki Mitani, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   48   S30 - S30   2007年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    DOI: 10.14841/jspp.2007.0.054.0

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  • Gene expression and functional analysis of a candidate gene related to Al-activated citrate transporter in barley 査読

    Jun Furukawa, Naoki Yamaji, Hua Wang, Maki Katsuhara, Kazuhiro Sato, Kazuyoshi Takeda, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   48   S44 - S44   2007年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

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  • Characterization of silicon permeability of NIP in gramineous plants 査読

    Namiki Mitani, Naoki Yamaji, Maki Katsuhara, Jian Feng Ma

    PLANT AND CELL PHYSIOLOGY   48   S30 - S30   2007年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

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  • Silicon uptake and accumulation in higher plants 査読

    Jian Feng Ma, Naoki Yamaji

    TRENDS IN PLANT SCIENCE   11 ( 8 )   392 - 397   2006年8月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE LONDON  

    Silicon (Si) accumulation differs greatly between plant species because of differences in Si uptake by the roots. Recently, a gene encoding a Si uptake transporter in rice, a typical Si-accumulating plant, was isolated. The beneficial effects of Si are mainly associated with its high deposition in plant tissues, enhancing their strength and rigidity. However, Si might play an active role in enhancing host resistance to plant diseases by stimulating defense reaction mechanisms. Because many plants are not able to accumulate Si at high enough levels to be beneficial, genetically manipulating the Si uptake capacity of the root might help plants to accumulate more Si and, hence, improve their ability to overcome biotic and abiotic stresses.

    DOI: 10.1016/j.tplants.2006.06.007

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  • Silicon uptake and accumulation in higher plants 査読

    Jian Feng Ma, Naoki Yamaji

    TRENDS IN PLANT SCIENCE   11 ( 8 )   392 - 397   2006年8月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE LONDON  

    Silicon (Si) accumulation differs greatly between plant species because of differences in Si uptake by the roots. Recently, a gene encoding a Si uptake transporter in rice, a typical Si-accumulating plant, was isolated. The beneficial effects of Si are mainly associated with its high deposition in plant tissues, enhancing their strength and rigidity. However, Si might play an active role in enhancing host resistance to plant diseases by stimulating defense reaction mechanisms. Because many plants are not able to accumulate Si at high enough levels to be beneficial, genetically manipulating the Si uptake capacity of the root might help plants to accumulate more Si and, hence, improve their ability to overcome biotic and abiotic stresses.

    DOI: 10.1016/j.tplants.2006.06.007

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  • Two promoters conferring active gene expression in vegetative nuclei of tobacco immature pollen undergoing embryogenic dedifferentiation 査読

    Naoki Yamaji, Masaharu Kyo

    PLANT CELL REPORTS   25 ( 8 )   749 - 757   2006年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    In order to visualize the specific state of tobacco pollen undergoing dedifferentiation from immature pollen to embryogenic cells, we established tobacco marker lines transgenic for a vital reporter gene regulated under the transcriptional control of an 840 bp fragment, named A22pro. This fragment was obtained from the 5'-flanking region of a gene corresponding to a cDNA named A22 that was previously isolated through differential screening from a cDNA library prepared from tobacco pollen undergoing dedifferentiation. The reporter gene, named H3sGFP, consisting of synthetic green fluorescent protein gene (sGFP) and tobacco H3 histone gene for nuclear localization, was designed to distinguish the gene expression in the generative cell from that in the vegetative cell in a pollen grain. The marker line produced pollen showing a green fluorescent signal in the generative nuclei (GN) but the expression level of the transgene was low. Pollen after culture for dedifferentiation showed an intense signal transiently in the vegetative nuclei (VN), at a specific developmental stage of pollen, with a rapid increase of expression level of the transgene. Serial observations revealed that all androgenic embryos originated from the pollen grains that had shown the signal in their VN. Thus, A22pro is originally functional in gametogenesis but is activated in VN of pollen undergoing embryogenic dedifferentiation. Additionally, we observed a gene expression pattern identical to that described above, using another 5'-flanking region of a gene for a cDNA, named B27pro, homologous to A22 as a promoter of the reporter gene.

    DOI: 10.1007/s00299-005-0076-3

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  • P-157 A SPECIFIC TRANSPORTER OF IRON (III)-PHYTOSIDEROPHORE, MUGINEIC ACID IN BARLEY ROOTS

    Murata Yoshiko, Ma Jian Feng, Yamaji Naoki, Ueno Daisei, Nomoto Kyosuke, Iwashita Takashi

    International Symposium on the Chemistry of Natural Products   2006   "P - 157"   2006年7月

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    記述言語:英語   出版者・発行元:天然有機化合物討論会  

    DOI: 10.24496/intnaturalprod.2006.0__P-157_

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  • A specific transporter for iron(III)-phytosiderophore in barley roots 査読

    Y Murata, JF Ma, N Yamaji, D Ueno, K Nomoto, T Iwashita

    PLANT JOURNAL   46 ( 4 )   563 - 572   2006年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    Iron acquisition of graminaceous plants is characterized by the synthesis and secretion of the iron-chelating phytosiderophore, mugineic acid (MA), and by a specific uptake system for iron(III)-phytosiderophore complexes. We identified a gene specifically encoding an iron-phytosiderophore transporter (HvYS1) in barley, which is the most tolerant species to iron deficiency among graminaceous plants. HvYS1 was predicted to encode a polypeptide of 678 amino acids and to have 72.7% identity with ZmYS1, a first protein identified as an iron(III)-phytosiderophore transporter in maize. Real-time RT-PCR analysis showed that the HvYS1 gene was mainly expressed in the roots, and its expression was enhanced under iron deficiency. In situ hybridization analysis of iron-deficient barley roots revealed that the mRNA of HvYS1 was localized in epidermal root cells. Furthermore, immunohistological staining with anti-HvYS1 polyclonal antibody showed the same localization as the mRNA. HvYS1 functionally complemented yeast strains defective in iron uptake on media containing iron(III)-MA, but not iron-nicotianamine (NA). Expression of HvYS1 in Xenopus oocytes showed strict specificity for both metals and ligands: HvYS1 transports only iron(III) chelated with phytosiderophore. The localization and substrate specificity of HvYS1 is different from those of ZmYS1, indicating that HvYS1 is a specific transporter for iron(III)-phytosiderophore involved in primary iron acquisition from soil in barley roots.

    DOI: 10.1111/j.1365-313X.2006.02714.x

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  • A specific transporter for iron(III)-phytosiderophore in barley roots 査読

    Y Murata, JF Ma, N Yamaji, D Ueno, K Nomoto, T Iwashita

    PLANT JOURNAL   46 ( 4 )   563 - 572   2006年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    Iron acquisition of graminaceous plants is characterized by the synthesis and secretion of the iron-chelating phytosiderophore, mugineic acid (MA), and by a specific uptake system for iron(III)-phytosiderophore complexes. We identified a gene specifically encoding an iron-phytosiderophore transporter (HvYS1) in barley, which is the most tolerant species to iron deficiency among graminaceous plants. HvYS1 was predicted to encode a polypeptide of 678 amino acids and to have 72.7% identity with ZmYS1, a first protein identified as an iron(III)-phytosiderophore transporter in maize. Real-time RT-PCR analysis showed that the HvYS1 gene was mainly expressed in the roots, and its expression was enhanced under iron deficiency. In situ hybridization analysis of iron-deficient barley roots revealed that the mRNA of HvYS1 was localized in epidermal root cells. Furthermore, immunohistological staining with anti-HvYS1 polyclonal antibody showed the same localization as the mRNA. HvYS1 functionally complemented yeast strains defective in iron uptake on media containing iron(III)-MA, but not iron-nicotianamine (NA). Expression of HvYS1 in Xenopus oocytes showed strict specificity for both metals and ligands: HvYS1 transports only iron(III) chelated with phytosiderophore. The localization and substrate specificity of HvYS1 is different from those of ZmYS1, indicating that HvYS1 is a specific transporter for iron(III)-phytosiderophore involved in primary iron acquisition from soil in barley roots.

    DOI: 10.1111/j.1365-313X.2006.02714.x

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  • A silicon transporter in rice 査読

    JF Ma, K Tamai, N Yamaji, N Mitani, S Konishi, M Katsuhara, M Ishiguro, Y Murata, M Yano

    NATURE   440 ( 7084 )   688 - 691   2006年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Silicon is beneficial to plant growth and helps plants to overcome abiotic and biotic stresses by preventing lodging ( falling over) and increasing resistance to pests and diseases, as well as other stresses(1-3). Silicon is essential for high and sustainable production of rice(4), but the molecular mechanism responsible for the uptake of silicon is unknown. Here we describe the Low silicon rice 1 (Lsi1) gene, which controls silicon accumulation in rice, a typical silicon-accumulating plant. This gene belongs to the aquaporin family(5) and is constitutively expressed in the roots. Lsi1 is localized on the plasma membrane of the distal side of both exodermis and endodermis cells, where casparian strips are located. Suppression of Lsi1 expression resulted in reduced silicon uptake. Furthermore, expression of Lsi1 in Xenopus oocytes showed transport activity for silicon only. The identification of a silicon transporter provides both an insight into the silicon uptake system in plants, and a new strategy for producing crops with high resistance to multiple stresses by genetic modification of the root's silicon uptake capacity.

    DOI: 10.1038/nature04590

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  • Functional characterization of a Si transporter gene Lsi1 in rice 査読

    N Mitani, N Yamaji, K Tamai, S Konishi, M Yano, JF Ma

    PLANT AND CELL PHYSIOLOGY   47   S61 - S61   2006年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

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  • Functional analysis of rice gene Lsi2 related to Si uptake 査読

    N Yamaji, JF Ma

    PLANT AND CELL PHYSIOLOGY   47   S61 - S61   2006年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    DOI: 10.14841/jspp.2006.0.149.0

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  • Isolation and characterization of Lsi1 査読

    N Mitani, K Tamai, S Konishi, M Yano, N Yamaji, M Kyo, JF Ma

    PLANT AND CELL PHYSIOLOGY   46   S82 - S82   2005年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

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  • Cloning and characterization of cDNAs associated with the embryogenic dedifferentiation of tobacco immature pollen grains 査読

    M Kyo, S Hattori, N Yamaji, P Pechan, H Fukui

    PLANT SCIENCE   164 ( 6 )   1057 - 1066   2003年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    We conducted differential screening to obtain cDNAs showing that gene expression is highly associated with the transformation from immature pollen to embryogenic cell, so-called embryogenic dedifferentiation of pollen, in a Nicotiana tabacum pollen culture system and analyzed their expression and sequences. Seventy-seven cDNA clones were independently isolated and distinguished into 16 groups based on their sequences. The groups were further categorized into two classes, Class I and II, based on the gene expression pattern of the representative clone of each group under various pollen culture conditions arranged for examining the coincidence with the dedifferentiation. The 13 groups in Class I showed prominent expression under the conditions allowing or facilitating pollen dedifferentiation and the expression level increased earlier than A-type cyclin genes, but they were not markedly expressed in the cell populations rich in S-phase cells, i.e. young anthers with pollen mother cells, BY-2 cells at the growth phase and early phase embryos derived from immature pollen. The other three groups in Class II encoded homologs to H1 histone, H2A histone and minichromosome maintenance (MCM) protein, respectively. The level of their transcripts increased during dedifferentiation. but it was also high in anthers containing pollen mother cells and in the proliferating BY-2 cells indicating that their expression is coincident with the S phase but not with dedifferentiation. These findings suggest that pollen dedifferentiation is a complex process accompanied with the reentrance of cell cycle and unknown events probably caused by specific expression of many genes, at least, listed in Class I. These genes should be used as reliable markers and important clues for further studies on the molecular mechanism of dedifferentiation. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.

    DOI: 10.1016/S0168-9452(03)00111-0

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  • Isolation of cDNAs coding for NtEPb1-b3, marker proteins for pollen dedifferentiation in a tobacco pollen culture system 査読

    M Kyo, N Yamaji, Y Yuasa, T Maeda, H Fukui

    PLANT SCIENCE   163 ( 5 )   1055 - 1061   2002年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    Several phosphoproteins, Nicotiana tabacum L. embryogenic pollen-abundant phosphoproteins (NtEPs), characteristically appear in the dedifferentiation process from immature pollen grains to embryogenic cells in a pollen culture system. Among NtEPs we focused our attention on three proteins (NtEPb1-b3) which showed the highest correlation with the dedifferentiation and possessed different pI values and similar molecular weights (ca. 22 kDa). Using probes designed from the N-terminal amino acid sequence common to the three, we isolated 14 clones of cDNA belonging to three similar sequences which probably correspond to NtEPb1-b3. The predicted amino acid sequences showed moderate homology to NtEPc, several type-1 copper-binding glycoproteins and a kind of early nodulin. The level of the transcripts for NtEPbs is highly associated with the pollen dedifferentiation but not with pollen maturation nor with cell division accompanied by meiosis or proliferation of BY-2 cells. Such an expression manner was distinguished from that of a gene coding for A-type cyclin (Ntcyc 25), indicating that NtEPb genes are not under cell cycle control. These results suggest that there exist genes related to an unknown event other than the reentrance of cell cycle in the dedifferentiation process of immature pollen that may be important for acquisition of embryogenic competence. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

    DOI: 10.1016/S0168-9452(02)00280-7

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  • Isolation of promoter elements conferring the expression pattern associated with the embryogenic dedifferentiation in a tobacco pollen culture system 査読

    N Yamaji, M Kyo

    PLANT AND CELL PHYSIOLOGY   43   S132 - S132   2002年

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

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▼全件表示

書籍等出版物

  • Cadmium Toxicity

    Wang, W., Yamaji, N. and Ma, J. F.( 担当: 共著)

    Springer, Singapore  2019年2月  ( ISBN:9789811336300

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    担当ページ:115-124   記述言語:英語

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  • Rice Genomics, Genetics and Breeding

    Mitani-Ueno, N., Yamaji, N. and Ma, J.F.( 担当: 共著)

    Springer, Singapore  2018年2月  ( ISBN:9789811074608

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    担当ページ:223-240   記述言語:英語

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MISC

  • イネのケイ素吸収を制御する長距離シグナルタンパク質の同定

    YAMAJI Naoki, MITANI-UENO Namiki, KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   64th   2023年

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  • 4-2-2 OsZIP2 mediates xylem loading and preferential distribution of Zn in rice(4-2 植物の微量栄養素 2022年度東京大会)

    黄 勝, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   68   45 - 45   2022年9月

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.68.0_45_2

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  • P4-1-2 蛍光染色によるイネ葉のケイ素蓄積過程の解析(4-1 植物の多量栄養素 2022年度東京大会)

    山地 直樹, 藤井 理樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   68   41 - 41   2022年9月

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.68.0_41_3

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  • 4-3-4 Duplication of a manganese/cadmium transporter gene leads to low cadmium accumulation in rice grain(4-3 植物の有害元素 2022年度東京大会)

    余 恩, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   68   54 - 54   2022年9月

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.68.0_54_1

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  • 4-1-5 Identification of a gene involved in loading phosphorus into barley grains(4-1 植物の多量栄養素 2022年度東京大会)

    黄 衡亮, 久野 裕, 黄 勝, 三谷 奈見季, 佐藤 和広, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   68   37 - 37   2022年9月

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.68.0_37_2

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  • 根におけるミネラルとその輸送体の可視化—Imaging of mineral elements and their transporters in plant roots—特集 根の見える化で作物を科学する

    馬 建鋒, 山地 直樹

    アグリバイオ = Agricultural biotechnology   6 ( 6 )   502 - 506   2022年6月

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    記述言語:日本語  

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  • オオムギ根内生微生物の同定と役割の解明

    木代勝元, 最相大輔, 山下純, 山地直樹, 山本敏央, 門田有希, 持田恵一, 中川智行, 谷明生

    日本農芸化学会大会講演要旨集(Web)   2022   2022年

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  • 根の見える化で作物を科学する 3 根におけるミネラルとその輸送体の可視化

    MA Jian Feng, 山地直樹

    アグリバイオ   6 ( 6 )   2022年

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  • イネの根における効率的なケイ酸ローディングに寄与する輸送体Lsi3

    HUANG Sheng, YAMAJI Naoki, SAKURAI Gen, MITANI-UENO Namiki, KONISHI Noriyuki, MA Jian Feng

    日本植物生理学会年会(Web)   63rd   2022年

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  • イネホウ素吸収の制御機構

    HUANG Sheng, KONISHI Noriyuki, YAMAJI Naoki, MITANI-UENO Namiki, MA Jian Feng

    日本植物生理学会年会(Web)   63rd   2022年

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  • オオムギ穀粒へのリンローディングに関与する遺伝子の同定

    黄衡亮, 久野裕, 黄勝, 三谷奈見季, 佐藤和広, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   68   2022年

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  • イネにおいてOsZIP2は木部の充填と亜鉛の選択的な分布を仲介する

    黄勝, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   68   2022年

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  • 蛍光染色によるイネ葉のケイ素蓄積過程の解析

    山地直樹, 藤井理樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   68   2022年

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  • 4-2-6 Role of OsLsi6 in B distribution under B-sufficient condition in rice(4-2 植物の微量栄養素 2021年度北海道大会)

    黄 勝, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   67   60 - 60   2021年9月

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.67.0_60_3

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  • P4-1-1 ケイ素蓄積に伴うイネ葉身のトランスクリプトーム解析(4-1 植物の多量栄養素 2021年度北海道大会)

    山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   67   52 - 52   2021年9月

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.67.0_52_3

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  • 4-2-15 Role of lateral roots and root hairs in high uptake of manganese and cadmium in rice(4-2 植物の微量栄養素 2021年度北海道大会)

    余 恩, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   67   63 - 63   2021年9月

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.67.0_63_3

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  • 4-3-12 A glycosyltransferase gene is required for tolerance to acidic stress in rice(4-3 植物の有害元素 2021年度北海道大会)

    王 珮同, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   67   72 - 72   2021年9月

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.67.0_72_3

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  • P4-1-3 イネのケイ素蓄積に関わる輸送体SIET4 の更なる解析(4-1 植物の多量栄養素 2021年度北海道大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   67   53 - 53   2021年9月

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.67.0_53_2

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  • 9. 亜鉛の輸送機構

    黄 勝, 山地 直樹, 馬 建鋒

    日本土壌肥料学雑誌   92 ( 2 )   136 - 140   2021年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dojo.92.2_136

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  • 12. 銅の輸送機構

    山地 直樹

    日本土壌肥料学雑誌   92 ( 2 )   155 - 159   2021年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dojo.92.2_155

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  • 植物の元素イメージング 査読

    馬 建鋒, 田野井 慶太朗, 古川 純, 鈴井 伸郎, Wang Peng, 山地 直樹, 高野 順平

    日本土壌肥料学雑誌   92 ( 2 )   213 - 218   2021年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dojo.92.2_213

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  • オオムギ根圏の共生微生物の単離と同定

    木代勝元, 最相大輔, 山下純, 山地直樹, 山本敏央, 門田有希, 持田恵一, 中川智行, 谷明生

    日本農芸化学会大会講演要旨集(Web)   2021   2021年

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  • イネにおける細胞壁多糖類のアルミニウム適応応答

    長山照樹, 斎藤大夢, 中村敦子, 山地直樹, 佐藤忍, 古川純, 岩井宏暁

    日本植物生理学会年会(Web)   62nd   2021年

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  • イネのケイ素蓄積に関わる輸送体SIET4の更なる解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021年

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  • イネのマンガンとカドミウムの高吸収における側根と根毛の役割

    余恩, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021年

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  • ケイ素蓄積に伴うイネ葉身のトランスクリプトーム解析

    山地直樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021年

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  • イネにおけるホウ素吸収の制御機構

    黄勝, 山地直樹, 小西範幸, 邵継鋒, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021年

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  • 穀物由来ケイ酸チャネルによるケイ酸輸送機構の構造学的研究

    齊藤恭紀, 三谷(上野)奈見季, 斉藤圭亮, 斉藤圭亮, 松木謙悟, 黄勝, 楊霊麗, 山地直樹, 石北央, 石北央, 沈建仁, 沈建仁, 馬建鋒, 菅倫寛, 菅倫寛, 菅倫寛

    膜シンポジウム(CD-ROM)   ( 33 )   2021年

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  • イネにおけるグリコシルトランスフェラーゼ遺伝子は酸ストレス耐性に必要である

    王珮同, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021年

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  • イネのホウ素十分条件下でのホウ素分布におけるOsLsi6の役割

    黄勝, 山地直樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   67   2021年

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  • イネ由来ケイ酸チャネルによるケイ酸透過機構の構造基盤

    齊藤恭紀, 三谷(上野)奈見季, 斉藤圭亮, 斉藤圭亮, 松木謙悟, HUANG Sheng, YANG Lingli, 山地直樹, 石北央, 石北央, SHEN Jian-Ren, SHEN Jian-Ren, MA Jian Feng, 菅倫寛, 菅倫寛, 菅倫寛

    日本分子生物学会年会プログラム・要旨集(Web)   44th   2021年

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  • オオムギ根から分離された新種細菌Rugamonas sp.の性質

    木代勝元, 最相大輔, 山下純, 山地直樹, 山本敏央, 門田有希, 持田恵一, 中川智行, 谷明生

    日本農芸化学会西日本支部大会およびシンポジウム講演要旨集   2021 (CD-ROM)   2021年

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  • Plant Nutrition for Human Nutrition: Hints from Rice Research and Future Perspectives

    Sheng Huang, Peitong Wang, Naoki Yamaji, Jian Feng Ma

    MOLECULAR PLANT   13 ( 6 )   825 - 835   2020年6月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:CELL PRESS  

    Both plants and humans require mineral elements for their healthy growth and development. Mineral elements in the soil are taken up by the plant roots and transported to the edible parts for human consumption through various different transporters. An ideal future crop for human health should be rich in essential mineral elements but with less toxic elements in the edible parts. However, due to the great difference in the numbers and amounts of mineral elements required between plants and humans, it is a challenge to balance plant growth and nutrient requirement for humans. In this article, we mainly focus on the transport system of mineral elements from soil to grain in rice, a staple food for half of the world's population, and discuss recent progress on the underlying genetic and physiological mechanisms. Examples are given for silicon, zinc, and iron essential/beneficial for both plants and humans, selenium and iodine only essential for humans, and toxic cadmium and arsenic for all organisms. Manipulation of some transporters for these elements, especially those localized in the node for allocation of mineral elements to the grain, has been successful in generating rice with higher density and bioavailability of essential elements but with less accumulation of toxic elements. We provide our perspectives toward breeding future crops for human health.

    DOI: 10.1016/j.molp.2020.05.007

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  • イネのZn吸収に関与するトランスポーターの同定

    黄勝, 佐々木明正, 山地直樹, 三谷奈見季, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   66   2020年

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  • ソバのマンガン高集積に関与するFeNramp5の機能解析

    横正健剛, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   66   2020年

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  • イネのマンガン欠乏感受性に関与する輸送体

    上野大勢, 古田富大, 三宅親弘, 車景, 山地直樹, 馬建鋒, 岩崎貢三

    日本土壌肥料学会講演要旨集(Web)   66   2020年

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  • ケイ酸輸送体Lsi2の相同遺伝子SIET3,4,5の機能解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   66   2020年

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  • なぜオオムギ属の花序の単位は三小穂なのか

    佐久間俊, 佐久間俊, MASCHER Martin, RAJARAMAN Jey, 山地直樹, 馬建鋒, 佐藤和広, 小松田隆夫, SCHNURBUSCH Thorsten

    日本植物学会大会研究発表記録(CD-ROM)   84th   2020年

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  • イネにおけるホウ素の優先的分配に関わる輸送体の更なる解析

    邵継鋒, 山地直樹, 黄勝, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   65   2019年

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  • イネの鉄分配に関与するOsOPT7の詳細解析

    山地直樹, 横正健剛, 佐々木明正, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   65   2019年

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  • イネにおけるケイ素輸送モデルによる輸送体配置の影響分析

    櫻井玄, 山地直樹, 三谷奈見季, 小野圭介, 横沢正幸, 酒井英光, 吉本真由美, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   65   2019年

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  • イネのLsi2相同遺伝子の機能解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   65   2019年

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  • イネのSiを改良したミネラルバランスにおけるグローバルトランスクリプトーム解析

    黄勝, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   65   2019年

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  • オオムギのCd蓄積を制御するQTL遺伝子の機能解析

    雷貴傑, 久野裕, 山地直樹, 佐藤和広, 馬建鋒

    日本土壌肥料学会講演要旨集(Web)   65   2019年

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  • イネのART1と相互作用するタンパク質OsBBPIsの機能解析

    横正健剛, 陳志長, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集   64   66   2018年8月

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    記述言語:日本語  

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  • イネにおける作物体内ミネラル輸送ダイナミクスの解析手法の開発

    櫻井玄, 小野圭介馬建鋒, 山地直樹, 三谷奈見季, 横沢正幸

    農業環境変動研究センター研究成果情報   2018年3月

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    記述言語:日本語  

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  • 低投入を目指した植物栄養生理

    樋口 恭子, 岡田 謙介, 鈴木 雄二, 和崎 淳, 山地 直樹, 三輪 京子, 小山 博之

    日本土壌肥料学雑誌   89 ( 3 )   266 - 271   2018年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • イネにおけるホウ素の優先的分配に関与する輸送体

    邵継鋒, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集   64   2018年

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  • トランスゴルジ局在型MTP11はイネのマンガン耐性に関与する

    常光優太, 宮崎彰, 山地直樹, 馬建鋒, 加藤伸一郎, 岩崎貢三, 上野大勢

    日本土壌肥料学会講演要旨集   64   2018年

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  • レーザーアブレーションICP-MSを用いたイネ節の維管束間輸送過程の解析

    山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集   64   2018年

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  • イネの高マンガン集積に関わる分子機構

    上野大勢, 常光優太, 山地直樹, 馬建鋒, 岩崎貢三

    日本森林学会大会学術講演集   129th   2018年

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  • 生物情報科学・細胞生物学的手法から見えてきた植物栄養応答(5)作物の金属ストレス耐性機構

    横正 健剛, 山地 直樹

    日本土壌肥料学雑誌 = Japanese journal of soil science and plant nutrition   88 ( 2 )   139 - 146   2017年4月

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    記述言語:日本語   出版者・発行元:日本土壌肥料学会 ; 1927-  

    DOI: 10.20710/dojo.88.2_139

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  • 4-2-10 ソバのNrampファミリー遺伝子の機能解析(4-2 植物の微量栄養素,2017年度仙台大会)

    横正 健剛, 邵 継鋒, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   63 ( 0 )   66 - 66   2017年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • A GDSL-motif esterase/acyltransferase/lipase is responsible for leaf water retention in barley.

    Li C, Chen G, Mishina K, Yamaji, N, Ma, J. F, Yukuhiro, F, Tagiri, A, Liu, C, Pourkheirandish, M, Anwar, N, Ohta, M, Zhao, P, qvist, U, Li, X, Konatsuda, T

    Plant Direct   1 ( 5 )   1 - 12   2017年

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  • オオムギの新規アルミニウム耐性制御機構

    馬建鋒, 柏野美帆, 山地直樹

    日本土壌肥料学会講演要旨集   63   2017年

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  • イネのケイ酸輸送体Lsi6の節における著しい高発現は組換えプロモーターでは再現できない

    山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集   63   2017年

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  • イネ組織間におけるミネラル輸送

    三谷奈見季, 山地直樹, 馬建鋒

    日本土壌肥料学会講演要旨集   63   2017年

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  • 4-3-9 Further characterization of two Al-inducible MATE genes in buckwheat(4-3 植物の有害元素,2016年度佐賀大会)

    雷 貴傑, 横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   62 ( 0 )   65 - 65   2016年

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    記述言語:英語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.62.0_65_3

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  • 4-3-18 イネ科作物の土壌pH適応におけるトレードオフの可能性(4-3 植物の有害元素,2016年度佐賀大会)

    山地 直樹, 柏野 美帆, 横正 健剛, 馬 建鋒

    日本土壌肥料学会講演要旨集   62 ( 0 )   68 - 68   2016年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.62.0_68_3

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  • 16 イネのケイ酸吸収に関与する輸送体 Lsi1 と Lsi2 の更なる解析(関西支部講演会,2015年度各支部会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   62 ( 0 )   284 - 284   2016年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.62.0_284_1

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  • P4-1-6 イネ由来ケイ酸輸送体Lsi1の更なる解析(ポスター,4-1 植物の多量栄養素,2016年度佐賀大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   62 ( 0 )   52 - 52   2016年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.62.0_52_3

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  • 膜タンパク質輸送能力の統計的逆推定プロトコルの開発

    櫻井玄, 馬建鋒, 山地直樹, 三谷奈見季

    農研機構農業環境変動研究センター成果情報(Web)   2016   2016年

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  • コメへの優先的リン分配を担うイネの新規リン酸輸送体

    山地直樹, 竹本侑馬, 宮地孝明, 三谷奈見季, 吉田薫, 馬建鋒

    トランスポーター研究会年会抄録集   11th   2016年

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  • Analysis of epigenetic marks on plant chromosomes -from micro to macro-

    Kiyotaka Nagaki, Naoki Yamaji, Keisuke Tanaka, Hisato Kobayashi, Minoru Murata

    GENES & GENETIC SYSTEMS   90 ( 6 )   374 - 374   2015年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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  • 4-3-16 Functional characterization of an Al-inducible expansin gene, OsEXPA10 in rice

    車 景, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 61 )   76 - 76   2015年9月

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    記述言語:英語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 4-1-2 イネ節で高発現する輸送体遺伝子OsSultr3;4の機能解析(4-1 植物の多量栄養素,2015年度京都大会)

    竹本 侑馬, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 61 )   48 - 48   2015年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 9 イネのマンガン耐性におけるOsMTP8の生理的意義(関西支部講演会,2014年度各支部会)

    上野 大勢, 小竹 咲也子, 竹本 侑馬, 山地 直樹, 馬 建鋒, 加藤 伸一郎, 岩崎 貢三

    日本土壌肥料学会講演要旨集   ( 61 )   278 - 278   2015年9月

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  • 11 イネのヒ素耐性と集積に関与する輸送体ABCC1の機能解析(関西支部講演会,2014年度各支部会)

    山木 智央, 山地 直樹, Song Won Yong, Lee Youngsook, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 61 )   279 - 279   2015年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 4-3-13 ヒ素輸送体遺伝子OsABCC1の発現量の品種間差解析(4-3 植物の有害元素,2015年度京都大会)

    馬 建鋒, 山地 直樹, 山木 智央

    日本土壌肥料学会講演要旨集   ( 61 )   75 - 75   2015年9月

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  • 4-2-12 MTP8とMTP8.1はイネの根におけるマンガン耐性に寄与する(4-2 植物の微量栄養素,2015年度京都大会)

    常光 優太, 竹本 侑馬, 山地 直樹, 馬 建鋒, Yusuyin Mayila, 加藤 伸一郎, 岩崎 貢三, 上野 大勢

    日本土壌肥料学会講演要旨集   ( 61 )   68 - 68   2015年9月

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  • 10 イネにおけるリンの分配に関与する輸送体OsSultr3;4の解析(関西支部講演会,2014年度各支部会)

    竹本 侑馬, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 61 )   279 - 279   2015年9月

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  • 葉緑体ビタミンCトランスポーターの同定とその生理的役割

    宮地孝明, 黒森崇, 竹内優, 山地直樹, 横正健剛, 嶋澤厚, 杉本絵理子, 表弘志, 馬建鋒, 篠崎一雄, 森山芳則, 森山芳則

    トランスポーター研究会年会抄録集   10th   41   2015年6月

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  • P4-3-11 アルミニウム処理下のソバの細胞膜と液胞膜のプロテオーム解析(ポスター,4-3 植物の有害元素,2015年度京都大会)

    横正 健剛, 雷 貴傑, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   61 ( 0 )   80 - 80   2015年

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  • 葉緑体のアスコルビン酸トランスポーターの同定とその生理的役割

    宮地孝明, 黒森崇, 竹内優, 山地直樹, 横正健剛, 嶋澤厚, 杉本絵理子, 表弘志, 馬建鋒, 篠崎一雄, 森山芳則

    日本薬学会年会要旨集(CD-ROM)   135th   ROMBUNNO.26P-PM12   2015年

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  • 4-3-15 Characterization of two Al-induced half-size ABC transporters, FeALS1 with different size in buckwheat

    雷 貴傑, 横正 健剛, 山地 直樹, 柏野 美帆, 馬 建鋒

    日本土壌肥料学会講演要旨集   61 ( 0 )   76 - 76   2015年

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    記述言語:英語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 酸性土壌を突破する植物の戦略

    山地直樹, 馬 建鋒

    化学と生物   53 ( 8 )   529 - 534   2015年

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    記述言語:日本語   出版者・発行元:公益社団法人 日本農芸化学会  

    DOI: 10.1271/kagakutoseibutsu.53.529

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  • 4-1-1 イネ節におけるケイ酸分配機構の統合解析(4-1 植物の多量栄養素,2015年度京都大会)

    山地 直樹, 櫻井 玄, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   61 ( 0 )   48 - 48   2015年

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  • イネのリン分配に関与する輸送体OsSultr3;4の機能解析

    竹本侑馬, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   56th   2015年

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  • 根におけるケイ素吸収・輸送モデルの開発とイネがケイ素を多く吸収できるメカニズムの解明

    櫻井玄, 佐竹暁子, 山地直樹, 横沢正幸, FEUGIER Francois Gabriel, 三谷奈見季, 馬建鋒

    農業環境技術研究所研究成果情報   31   2015年

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  • 亜鉛欠乏時に根で高発現するイネOsZIP9の機能解析

    佐々木明正, 柏野美帆, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   56th   2015年

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  • 4-2-11 A possible chloride transporter required for root growth in rice

    陳 志長, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 60 )   64 - 64   2014年9月

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  • 9 イネの亜鉛分配に関与する輸送体遺伝子OsZIP3の機能解析(関西支部講演会,2013年度各支部会)

    佐々木 明正, 山地 直樹, 柏野 美帆, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 60 )   289 - 289   2014年9月

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  • 4-1-26 Dissection of Si-decreased Mn accumulation in rice shoots

    車 景, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 60 )   55 - 55   2014年9月

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  • 11 イネのヒ素耐性と集積に関与する輸送体ABCC1(関西支部講演会,2013年度各支部会)

    山木 智央, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 60 )   290 - 290   2014年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 4-2-3 イネの鉄分配に関与するOsOPT7の解析(4-2 植物の微量栄養素,2014年度東京大会)

    山地 直樹, 佐々木 明正, 柏野 美帆, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 60 )   61 - 61   2014年9月

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  • 4-3-2 イネヒ素集積のQTL解析(4-3 植物の有害元素,2014年度東京大会)

    馬 建鋒, 山地 直樹, 竹本 侑馬, 福岡 修一, 正村 純彦, 安藤 露

    日本土壌肥料学会講演要旨集   60 ( 60 )   68 - 68   2014年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

    DOI: 10.20710/dohikouen.60.0_68_2

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  • 13 Involvement of a magnesium transporter OsMGT1 in alleviating aluminum and salt stress in rice

    Chen Zhi Chang, Yamaji Naoki, Ma Jian Feng

    日本土壌肥料学会講演要旨集   ( 60 )   291 - 291   2014年9月

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  • I-2 環境変動に対する輸送体の応答機構(I 植物栄養と数理モデルの接点-数理モデルで植物栄養の仕組を理解する,シンポジウム,2014年度東京大会)

    山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 60 )   188 - 188   2014年9月

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  • 8 クエン酸輸送体OsFRDL1はイネの地上部の鉄分配にも関与する(関西支部講演会,2013年度各支部会)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   60 ( 0 )   289 - 289   2014年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 4-3-17 アルミニウム集積植物ソバのAl応答遺伝子の網羅的発現解析(4-3 植物の有害元素,2014年度東京大会)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   60 ( 0 )   73 - 73   2014年

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  • 1-5 イネにおけるケイ素吸収・輸送モデルによる植物体構造とその意義の解明(I 植物栄養と数理モデルの接点-数理モデルで植物栄養の仕組を理解する,シンポジウム,2014年度東京大会)

    櫻井 玄, 佐竹 暁子, 山地 直樹, 三谷 奈見季, 横沢 正幸, 馬 建鋒

    日本土壌肥料学会講演要旨集   60 ( 0 )   191 - 191   2014年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 10 イネ野生種におけるケイ酸吸収機構に関する研究(関西支部講演会,2013年度各支部会)

    三谷 奈見季, 大貝 久夫, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   60 ( 0 )   290 - 290   2014年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 14 イネのマンガン集積に関与する輸送体OsMTP8の解析(関西支部講演会,2013年度各支部会)

    竹本 侑馬, 三谷 奈見季, 佐々木 明正, 山地 直樹, 馬 建鋒, 加藤 伸一郎, 岩崎 貢三, 上野 大勢

    日本土壌肥料学会講演要旨集   60 ( 0 )   291 - 291   2014年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • OsHMA3輸送体の組織局在の改変による低カドミウム米作出の試み

    馬建鋒, 夏継星, 山地直樹

    日本植物生理学会年会要旨集   55th   2014年

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  • イネ節における鉄の分配に関与する輸送体の探索と機能解析

    山地直樹, 佐々木明正, 柏野美帆, 馬建鋒

    日本植物生理学会年会要旨集   55th   2014年

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  • イネ科作物の量的形質遺伝子の同定 第4章 イネの生理形態に関する形質の解析 5 イネのカドミウム集積に関与する遺伝子の単離と解析(QTL4005)

    馬建鋒, 山地直樹

    農林水産省農林水産技術会議事務局研究成果   ( 512 )   2014年

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  • イネのヒ素耐性と分配に関与する輸送体の解析

    山木智央, 山地直樹, SONG Won Yong, LEE Youngsook, 馬建鋒

    日本植物生理学会年会要旨集   55th   2014年

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  • イネの節で局在する輸送体OsZIP3は亜鉛の優先的分配に関与する

    佐々木明正, 山地直樹, 柏野美帆, 三谷奈見季, 馬建鋒

    日本植物生理学会年会要旨集   55th   2014年

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  • イネの質的形質遺伝子の単離と機能解明 第1章 おもな個別研究課題の成果 2 酸化還元ストレス,カドミウム,栄養不足等の環境ストレス耐性に係わる主要な質的形質遺伝子に関する研究(2)イネのケイ酸輸送とアルミニウム耐性に関与する遺伝子の単離と機能解析(IPG0006)

    馬建鋒, 山地直樹

    農林水産省農林水産技術会議事務局研究成果   ( 511 )   2014年

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  • イネのケイ素輸送におけるカスパリー線の役割とトランスポーター配置

    櫻井玄, 馬建鋒, 佐竹暁子, 横沢正幸, 山地直樹, 三谷奈見季

    日本植物生理学会年会要旨集   55th   2014年

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  • ミネラルトランスポーターの組織・細胞局在に関する研究(日本土壌肥料学会奨励賞受賞)

    山地 直樹

    日本土壌肥料學雜誌   84 ( 5 )   353 - 354   2013年10月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

    DOI: 10.20710/dojo.84.5_353

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  • 奨-4 ミネラルトランスポーターの組織・細胞局在に関する研究(日本土壌肥料学会奨励賞記念講演,2013年度名古屋大会)

    山地 直樹

    日本土壌肥料学会講演要旨集   ( 59 )   233 - 233   2013年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 4-1-9 Characterization of nutrient uptake in a novel short-root mutant of rice

    夏 継星, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 59 )   56 - 56   2013年9月

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    記述言語:英語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 4-2-3 Translocation of Cu from roots to shoots is mediated by OsHMA5 in rice

    〓 鋒林, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 59 )   67 - 67   2013年9月

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  • 4-2-5 節を介した亜鉛の分配に関与する輸送体OsZIP3(4-2 植物の微量栄養素)

    佐々木 明正, 山地 直樹, 柏野 美帆, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 59 )   68 - 68   2013年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • イネの銅再転流に関わる輸送体OsYSL16の解析

    鄭録慶, 山地直樹, 横正健剛, 馬建鋒

    日本植物生理学会年会要旨集   54th   153   2013年3月

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  • イネの節におけるミネラルの維管束間輸送と分配制御機構

    山地直樹, 佐々木明正, 鄭録慶, 夏継星, 横正健剛, 三谷奈見季, 馬建鋒

    トランスポーター研究会年会抄録集   8th   47   2013年

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  • 4-2-4 イネ銅の再転流に関与するトランスポーターの同定(4-2 植物の微量栄養素)

    鄭 録慶, 山地 直樹, 横正 健剛, 馬 建鋒

    日本土壌肥料学会講演要旨集   59 ( 0 )   68 - 68   2013年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P4-2-3 イネ地上部の鉄分配に関与するクエン酸トランスポーターOsFRDL1(4-2 植物の微量栄養素)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   59 ( 0 )   73 - 73   2013年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 4-1-2 野生イネ由来ケイ酸トランスポーターの解析(4-1 植物の多量栄養素)

    三谷 奈見季, 大貝 久生, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   59 ( 0 )   54 - 54   2013年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • ゼニゴケ発現系を用いた植物由来輸送体の輸送活性測定法の確立

    佐々木明正, 石崎公庸, 山地直樹, 河内孝之, 馬建鋒

    日本植物生理学会年会要旨集   54th   2013年

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  • イネ由来ケイ酸輸送体Lsi1の発現応答およびその制御機構の解析

    三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   54th   2013年

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  • イネ節の維管束間輸送に働くケイ酸輸送体

    山地直樹, 三谷(上野)奈見季, 馬建鋒

    日本植物生理学会年会要旨集   54th   2013年

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  • P10-12 OsNramp3によるマンガン分配制御機構(10.植物の微量栄養素,2012年度鳥取大会)

    佐々木 明正, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 58 )   70 - 70   2012年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • P9-1 イネケイ酸輸送体Lsi2(SIET1)のホモログSIET3-5の解析(9.植物の多量栄養素,2012年度鳥取大会)

    久家 徳之, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 58 )   58 - 58   2012年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 10-14 イネのOsHMA2による亜鉛およびカドミウムの選択的分配機構(10.植物の微量栄養素,2012年度鳥取大会)

    山地 直樹, 夏 継星, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 58 )   66 - 66   2012年9月

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  • 近年の農業へのケイ酸利用と研究 : 1. 植物のケイ素栄養と輸送

    山地 直樹, 馬 建鋒

    日本土壌肥料學雜誌   83 ( 3 )   319 - 325   2012年6月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

    DOI: 10.20710/dojo.83.3_319

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  • 近年の農業へのケイ酸利用と研究 : 1.植物のケイ素栄養と輸送

    山地 直樹, 馬 建鋒

    日本土壌肥料學雜誌 = Journal of the science of soil and manure, Japan   83 ( 3 )   319 - 325   2012年6月

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dojo.83.3_319

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  • ソバのアルミニウム誘導性輸送体遺伝子(FeIREG2)の機能解析

    横正健剛, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   53rd   303   2012年3月

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  • 葉の水分保持に関与するオオムギのEibil遺伝子の単離と機能解析

    CHEN G, 小松田隆夫, MA J, NAWRATH C, POURKHEIRANDISH M, 田切明美, HU Y, SAMERI M, LI X, ZHAO X, LIU Y, LI C, MA X, WANG A, NAIR S, WANG N, 宮尾安藝雄, 佐久間俊, 山地直樹, ZHENG X, NEVO E

    農業生物資源研究所主な研究成果   2011   2012年

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  • 11-11 オオムギのアルミニウム耐性機構の獲得(11.植物の有害元素,2012年度鳥取大会)

    柏野 美帆, 横正 健剛, 山地 直樹, 最相 大輔, 山根 美樹, 高橋 宏和, 佐藤 和広, 中園 幹生, 馬 建鋒

    日本土壌肥料学会講演要旨集   58 ( 0 )   76 - 76   2012年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 11-9 イネのMATE遺伝子OsFRDL2の更なる機能解析(11.植物の有害元素,2012年度鳥取大会)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   58 ( 0 )   75 - 75   2012年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • HD-ZIP I型転写因子の機能分化によるオオムギ側列小花の発達抑制

    佐久間俊, 佐久間俊, POURKHEIRANDISH Mohammad, GOETZ Hensel, JOCHEN Kumkehn, NILS Stein, THOMAS Wicker, 田切明美, 金森裕之, 松本隆, 山地直樹, MA J., 小松田隆夫, 小松田隆夫

    育種学研究   14   2012年

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  • P9-3 ケイ酸輸送体Lsi1のケイ酸応答性および組織局在性を制御するプロモーターの解析(9.植物の多量栄養素,2012年度鳥取大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   58 ( 0 )   59 - 59   2012年

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  • OsNramp3によるイネの選択的マンガン分配機構

    山地直樹, 佐々木明正, 夏継星, 馬建鋒

    日本植物生理学会年会要旨集   53rd   2012年

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  • 10-17 イネのマンガン分配に関与するOsNramp3の機能解析(10.植物の微量栄養素)

    山地 直樹, 佐々木 明正, 夏 継星, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 57 )   74 - 74   2011年8月

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  • 10-25 イネのマンガン吸収に関与する輸送体遺伝子の機能解析(10.植物の微量栄養素)

    佐々木 明正, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 57 )   77 - 77   2011年8月

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  • 11-30 Identification of the transporter involved in Mn tolerance in rice

    陳 宗慧, 神谷 岳洋, 岩崎 貢三, 加藤 伸一郎, 山地 直樹, 馬 建鋒, 上野 大勢

    日本土壌肥料学会講演要旨集   57 ( 0 )   88 - 88   2011年

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    記述言語:英語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.57.0_88_3

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  • ソバのアルミニウム誘導性輸送体遺伝子の単離と機能解析

    横正 健剛, 山地 直樹, 馬 建鋒

    日本植物生理学会年会およびシンポジウム 講演要旨集   2011 ( 0 )   960 - 960   2011年

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    出版者・発行元:日本植物生理学会  

    ソバはアルミニウム(Al)耐性が強いだけではなく、地上部に高濃度のAlを集積する。生理学的な解析から、ソバはAlに応答し根からシュウ酸を分泌し、地上部ではAlをシュウ酸との錯体で液胞に隔離することが明らかとなっている。これらの過程には多くのトランスポーターの関与が考えられるが未だ同定されていない。私たちはソバのAl耐性及び集積の分子機構を網羅的に解析するために、Alで処理したソバの根からRNAを抽出し次世代シークエンサーを用いてEST解析を行った。均一化したcDNAライブラリーから遺伝子配列の決定を行い約10万のコンティグを作成した。また、均一化していないcDNAライブラリーを用いたショートリード解析から発現量の測定を行った。本研究では、トランスポーター候補遺伝子の中で発現上位にあるFeIREG2についてその全長の単離と機能解析を行った。RACE法を用いて完全長cDNAを単離したところ、FeIREG2はシロイヌナズナのFeとCo、Niの排出トランスポーターAtIREG2とアミノ酸レベルで約60%の相同性を示した。FeIREG2は根で発現し、Al処理6時間後には約20倍に増加していた。この遺伝子とGFPの融合遺伝子をタマネギ表皮細胞に発現させたところ、液胞膜に局在していた。これらのことからFeIREG2はAlの液胞への隔離に関与している可能性が考えられ、更なる解析を進めている。

    DOI: 10.14841/jspp.2011.0.0960.0

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  • 11-27 ソバのアルミニウム誘導性トランスポーターの単離と機能解析(11.植物の有害元素)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   57 ( 0 )   87 - 87   2011年

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  • Silicon efflux transporters isolated from two pumpkin cultivars contrasting in Si uptake.

    Mitani-Ueno, N, Yamaji, N, Ma, J. F

    Plant Signaling & Behavior   6 ( 7 )   991 - 994   2011年

  • 9-15 オオムギ低親和性硝酸トランスポーターの機能解析(9.植物の多量栄養素)

    石川 伸二, 園部 和幸, 三谷 奈見季, 山地 直樹, 馬 建鋒, 大山 卓爾, 末吉 邦

    日本土壌肥料学会講演要旨集   57 ( 0 )   62 - 62   2011年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-28 ケイ酸輸送体Lsi1の発現制御機構の解析(9.植物の多量栄養素)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   57 ( 0 )   67 - 67   2011年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 11-38 イネ亜ヒ酸排出能の品種間差(11.植物の有害元素)

    大貝 久生, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   57 ( 0 )   91 - 91   2011年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • イネアルミニウム応答性転写調節因子ART1のシス配列の同定

    筒井友和, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   52nd   2011年

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  • イネのカドミウム集積を抑制する遺伝子

    馬建鋒, 上野大勢, 山地直樹, 矢野昌裕

    日本植物生理学会年会要旨集   52nd   2011年

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  • イネの節で高発現するOsNramp3の機能解析

    山地直樹, 夏継星, 佐々木明正, 馬建鋒

    日本植物生理学会年会要旨集   52nd   2011年

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  • トランスポーター研究が・肥料・植物栄養学にもたらすインパクト : 3.アルミニウム耐性に関わるトランスポーター

    馬 建鋒, 山地 直樹

    日本土壌肥料學雜誌   81 ( 5 )   518 - 522   2010年10月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

    DOI: 10.20710/dojo.81.5_518

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  • 11-16 オオムギアルミニウム活性型クエン酸トランスポーター遺伝子HvAACT1の発現調節機構(11.植物の有害元素,2010年度北海道大会)

    藤井 美帆, 山地 直樹, 中園 幹生, 佐藤 和広, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 56 )   80 - 80   2010年9月

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  • 11-4 イネ玄米のカドミウム集積を制御するトランスポーターの同定(11.植物の有害元素,2010年度北海道大会)

    上野 大勢, 山地 直樹, 河野 いづみ, 黄 朝鋒, 安藤 露, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 56 )   76 - 76   2010年9月

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  • 11-24 イネOsYSL6はマンガン過剰耐性に関与する(11.植物の有害元素,2010年度北海道大会)

    佐々木 明正, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 56 )   82 - 82   2010年9月

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  • 11-14 イネアルミニウム耐性遺伝子OsALS1の機能解析(11.植物の有害元素,2010年度北海道大会)

    黄 朝鋒, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 56 )   79 - 79   2010年9月

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  • 植物におけるミネラル関連トランスポーター

    馬 建鋒, 山地 直樹

    植物の生長調節   45 ( 1 )   49 - 57   2010年5月

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    記述言語:日本語   出版者・発行元:植物化学調節学会  

    Plants are characterized by mineral nutrition, which require 17 essential elements for their growth. Among them, 14 are minerals. These minerals are taken up by the roots, translocated from the roots to the shoots and finally distributed to different cells for functions. A large number of transporters is required for these transport processes. On the other hand, plants are exposed to toxic minerals, therefore detoxification of these minerals by plants at different cellular levels is also required for survival. Herein, we reviewed recent progresses in identification of transporters involved in uptake, translocation, and distribution of essential minerals and of transporters involved in the detoxification of toxic minerals, focusing on iron (Fe), silicon (Si) and aluminum (Al), which represent essential, beneficial and toxic minerals, respectively.

    DOI: 10.18978/jscrp.45.1_49

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  • ライムギ由来MATE遺伝子(ScFRDL1とScFRDL2)の単離と機能解析

    横正 健剛, 山地 直樹, 馬 建鋒

    日本植物生理学会年会およびシンポジウム 講演要旨集   2010 ( 0 )   679 - 679   2010年

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    出版者・発行元:日本植物生理学会  

    Multidrug and toxic compound extrusion(MATE)は排出型の輸送体で生物界に広く存在する。近年、いくつかの植物のMATE遺伝子は外向きのクエン酸輸送タンパク質をコードすることが報告されている。&lt;br&gt;ライムギはAl耐性の強い種とされその耐性は根からの有機酸(リンゴ酸、クエン酸)分泌に由来する。しかし分泌に関与する遺伝子は単離されていない。本研究ではライムギから2つのMATE遺伝子(ScFRDL1,ScFRDL2)を単離し機能解析を行った。まず、オオムギやイネのMATE遺伝子をもとにライムギMATE遺伝子の完全長cDNA配列を決定した。単離されたScFRDL1はオオムギのAl活性型クエン酸トランスポーターHvAACT1とアミノ酸配列で94%、ScFRDL2はイネのAl誘導型MATE遺伝子OsFRDL2と80%の相同性を示した。次に、遺伝子の発現解析を行った。ScFRDL1はAlによる発現の誘導は受けず、鉄欠乏に誘導された。また根の基部側では中心柱に局在していた。一方、ScFRDL2はAlにより発現が誘導され、根からのクエン酸分泌量もこの発現と同様に増加していた。しかし、鉄欠乏による影響は受けなかった。これらの結果からScFRDL1はクエン酸を導管に排出し地上部への鉄輸送に、ScFRDL2はAlによる根圏へのクエン酸分泌に関与することを示している。

    DOI: 10.14841/jspp.2010.0.0679.0

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  • 11-17 オオムギHvAACT1の5-UTRの挿入配列の解析 : イネによる検証(11.植物の有害元素,2010年度北海道大会)

    横正 健剛, 藤井 美帆, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   56 ( 0 )   80 - 80   2010年

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  • 9-3 イネケイ酸輸送体遺伝子Lsi1を過剰発現したシロイヌナズナの解析(9.植物の多量栄養素,2010年度北海道大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   56 ( 0 )   54 - 54   2010年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-4 イネケイ酸輸送体Lsi3の解析(9.植物の多量栄養素,2010年度北海道大会)

    山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   56 ( 0 )   55 - 55   2010年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • イネの新規ケイ酸輸送体Lsi3の解析

    山地直樹, 三谷奈見季, 馬建鋒

    日本植物生理学会年会要旨集   51st   2010年

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  • オオムギのケイ素分配に関与するトランスポーターの解析

    馬建鋒, 山地直樹, 千葉由佳子, 三谷奈見季

    日本植物生理学会年会要旨集   51st   2010年

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  • イネアルミニウムトランスポーターの同定

    夏継星, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   51st   2010年

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  • イネのカドミウム集積に関与する輸送体CASTLE1の単離と解析

    上野大勢, 山地直樹, 河野いづみ, 黄朝峰, 安藤露, 矢野昌裕, 馬建鋒

    日本植物生理学会年会要旨集   51st   2010年

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  • オオムギとイネのケイ酸吸収機構の違いに関する研究

    三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   51st   2010年

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  • 9-19 イネのケイ素分配の分子機構(I)(9.植物の多量栄養素,2009年度京都大会)

    山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 55 )   71 - 71   2009年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 11-23 Cd超集積植物Thlaspi caerulescens(ecotype,Ganges)由来のP-type ATPase HMA3の機能解析(11.植物の有害元素,2009年度京都大会)

    上野 大勢, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 55 )   96 - 96   2009年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 11-10 Cloning and functional analysis of a rice gene involved in the tolerance to multiple metal toxicity

    黄 朝鋒, 山地 直樹, 田島 茂行, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 55 )   92 - 92   2009年9月

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    記述言語:英語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 11-5 A rice Nramp gene is involved in Al tolerance

    Xia Jixing, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 55 )   90 - 90   2009年9月

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    記述言語:英語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • イネアルミニウム耐性におけるOsFRDL4の機能解析

    横正 健剛, 山地 直樹, 馬 建鋒

    日本植物生理学会年会およびシンポジウム 講演要旨集   2009 ( 0 )   436 - 436   2009年

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    出版者・発行元:日本植物生理学会  

    Multidrug and toxic compound extrusion(MATE)は排出型のトランスポーターであり生物界に広く存在することが知られている。近年、私たちはオオムギのMATE遺伝子HvAACT1がクエン酸トランスポーターであり、アルミニウムによる根圏へのクエン酸分泌に関与していることを報告した。HvAACT1のイネでの相同遺伝子は五つあり、そのうちの一つであるOsFRDL1は導管へクエン酸を輸送し、根から地上部への鉄輸送に関与することを明らかにした。本研究ではイネのもう一つの相同遺伝子、OsFRDL4の機能解析について報告する。まず、OsFRDL4のTos-17挿入株(NF)を取得し生理学的手法を用いて解析を行った。その結果、NFではOsFRDL1の破壊株のようなクロロシスや導管液中のクエン酸の低下などはみられなかった。次に、Alによる根圏へのクエン酸の分泌量を比較したところ、NFはWTに比べて著しく低下していることが明らかとなった。また破壊株のアルミニウム耐性は野生型と比べ、少し低下していた。これらのことはOsFRDL4がOsFRDL1と異なり、HvAACT1のようにAlによる根圏へのクエン酸の分泌に機能していることを示している。OsFRDL4は主に根で発現し、その発現量はHvAACT1とは異なり、アルミニウムによって数時間で100倍程度に増加していた。

    DOI: 10.14841/jspp.2009.0.0436.0

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  • 10-4 イネの鉄輸送とアルミニウム無毒化におけるOsFRDL2の役割(10.植物の微量栄養素,2009年度京都大会)

    横正 健剛, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   55 ( 0 )   81 - 81   2009年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 22 カボチャ及びトマトのケイ酸吸収特性と関連遺伝子の単離(関西支部講演会,2008年度各支部会)

    馬 建鋒, 吾郷 幸子, 山地 直樹, 三谷 奈見季, 岩崎 貢三

    日本土壌肥料学会講演要旨集   55 ( 0 )   298 - 298   2009年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-20 ブルームレスキュウリ用カボチャ台木のケイ酸吸収特性のさらなる解析(9.植物の多量栄養素,2009年度京都大会)

    馬 建鋒, 吾郷 幸子, 山地 直樹, 三谷 奈見季, 岩崎 貢三

    日本土壌肥料学会講演要旨集   55 ( 0 )   71 - 71   2009年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 44Kマイクロアレイによるイネアルミニウム応答遺伝子の網羅的解析

    筒井友和, 黄朝鋒, 山地直樹, 長村吉晃, 馬建鋒

    日本植物生理学会年会要旨集   50th   2009年

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  • カドミウム超集積植物Thlaspi caerulescens(ecotype,Ganges)由来のP-type ATPase HMA3の単離と機能解析

    上野大勢, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   50th   2009年

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  • オオムギアルミニウム活性型クエン酸トランスポーター遺伝子(HvAACT1)の発現調節機構に関する研究

    藤井美帆, 山地直樹, 佐藤和広, 馬建鋒

    日本植物生理学会年会要旨集   50th   2009年

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  • オオムギ由来排出型ケイ酸輸送体HvLsi2の更なる機能解析

    三谷奈見季, 山地直樹, 千葉由佳子, 馬建鋒

    日本植物生理学会年会要旨集   50th   2009年

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  • ケイ酸輸送体Lsi6によるイネ生殖生長期のケイ酸分配

    山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   50th   2009年

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  • III-5 ケイ酸輸送体と吸収・集積機構(III 養分吸収の分子メカニズム-どこまでわかったか、何がまだわかっていないか-,シンポジウム,2008年度愛知大会)

    山地 直樹

    日本土壌肥料学会講演要旨集   ( 54 )   223 - 223   2008年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 9-24 イネ転写調節因子STAR3によるアルミニウム耐性遺伝子の発現制御(9.植物の無機栄養,2008年度愛知大会)

    山地 直樹, 黄 朝鋒, 長村 吉晃, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 54 )   77 - 77   2008年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • P9-5 新規アルミニウム感受性突然変異体の単離と原因遺伝子のマッピング(ポスター紹介,9.植物の無機栄養,2008年度愛知大会)

    黄 朝鋒, 山地 直樹, 田島 茂行, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 54 )   86 - 86   2008年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • イネの鉄栄養に関与するクエン酸トランスポーターOsFRDL1の機能解析

    横正 健剛, 上野 大勢, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本植物生理学会年会およびシンポジウム 講演要旨集   2008 ( 0 )   27 - 27   2008年

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    出版者・発行元:日本植物生理学会  

    近年本研究室からオオムギのアルミニウム耐性に関わる遺伝子HvAACT1が同定された。この遺伝子はmultidrug and toxin excrusion (MATE)ファミリーに属しており、アルミニウム活性型クエン酸トランスポーターである。イネにおいてHvAACT1の相同遺伝子は3つあるが、今回はもっとも相同性の高い遺伝子(アミノ酸レベルで87%)であるOsFRDL1の機能について報告する。まずはOsFRDL1のTos-17の破壊株を取得して、アルミニウムによるクエン酸の分泌量を野生型と比較した。その結果、クエン酸の分泌量に差が認められなかった。次に、異なる鉄濃度(0.2μMと10μM FeSO4)で栽培したところ、0.2μM の場合のみ、破壊株の新葉にクロロシスが観察された。地上部の鉄濃度を比較した結果、破壊株の鉄濃度は野生株より低く、導管液中の鉄濃度とクエン酸濃度も野生型の約半分であった。また破壊株の根の中心柱に鉄の沈積が観察された。この遺伝子をアフリカツメガエルの卵母細胞に発現させたところ、クエン酸の外向きの輸送活性が認められた。この遺伝子は主に根で発現していて、その発現量は鉄の有無によってほとんど影響を受けなかった。これらの結果はOsFRDL1はオオムギのHvAACT1とは異なり、中心柱へクエン酸を輸送するために必要なトランスポーターであることを示している。このトランスポーターは鉄をクエン酸との錯体の形態で地上部に輸送するのに必要である。

    DOI: 10.14841/jspp.2008.0.0027.0

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  • P9-31 異なるイネ科植物由来のケイ酸トランスポーター特性の比較解析(ポスター紹介,9.植物の無機栄養,2008年度愛知大会)

    三谷 奈見季, 千葉 由佳子, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   54 ( 0 )   95 - 95   2008年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-23 植物の金属ストレスにおけるMATE遺伝子の機能解析(9.植物の無機栄養,2008年度愛知大会)

    横正 健剛, 上野 大勢, 山地 直樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集   54 ( 0 )   77 - 77   2008年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-26 イネのヒ素吸収に関与するトランスポーターの同定(9.植物の無機栄養,2008年度愛知大会)

    馬 建鋒, 山地 直樹, 三谷 奈見季, Zhao Fangjie

    日本土壌肥料学会講演要旨集   54 ( 0 )   78 - 78   2008年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P9-30 カボチャ2品種のケイ酸吸収特性の解析(ポスター紹介,9.植物の無機栄養,2008年度愛知大会)

    吾郷 幸子, 三谷 奈見季, 山地 直樹, 岩崎 貢三, 馬 建鋒

    日本土壌肥料学会講演要旨集   54 ( 0 )   95 - 95   2008年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • オオムギにおけるケイ酸トランスポーターの解析

    千葉由佳子, 三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   49th   2008年

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  • イネのヒ素吸収におけるケイ酸輸送体の関与

    山地直樹, 三谷奈見季, XU Xiao-Yan, MCGRATH Steve P., ZHAO Fang-Jie, 馬建鋒

    日本植物生理学会年会要旨集   49th   2008年

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  • イネのアルミニウム耐性に関与する細菌タイプABCトランスポーターの解析

    馬建鋒, 山地直樹, 黄朝鋒, 三谷奈見季

    日本植物生理学会年会要旨集   49th   2008年

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  • トウモロコシのケイ酸トランスポーターの同定

    三谷奈見季, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   49th   2008年

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  • 9-4 Functional analyses of two Al-tolerant genes Als1 and Als2 in rice

    黄 朝鋒, 山地 直樹, 田島 茂行, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 53 )   64 - 64   2007年8月

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    記述言語:英語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • イネのケイ素トランスポーター.

    馬 建鋒, 山地直樹, 三谷奈見季

    タンパク質核酸酵素   52   1849 - 1856   2007年

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  • 9-6 オオムギにおけるアルミニウムによるクエン酸分泌に関与する遺伝子の単離と機能解析(9.植物の無機栄養,2007年度東京大会)

    古川 純, 山地 直樹, 王 華, 三谷 奈見季, 村田 佳子, 佐藤 和広, 且原 真木, 武田 和義, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   64 - 64   2007年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-41 トウモロコシ由来のケイ酸吸収遺伝子の単離と解析(9.植物の無機栄養,2007年度東京大会)

    三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   76 - 76   2007年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P9-40 大麦におけるケイ酸吸収機構の解析(9.植物の無機栄養,2007年度東京大会)

    千葉 由佳子, 三谷 奈見季, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   89 - 89   2007年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • P9-41 イネのケイ酸輸送体と吸収・集積機構(9.植物の無機栄養,2007年度東京大会)

    山地 直樹, 三谷 奈見季, 玉井 一規, 馬 建鋒

    日本土壌肥料学会講演要旨集   53 ( 0 )   90 - 90   2007年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • トウモロコシ由来の鉄-ムギネ酸錯体輸送体遺伝子ZmYS1の更なる解析

    上野大勢, 山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   48th   2007年

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  • イネケイ酸輸送体Lsi6の機能解析

    山地直樹, 三谷奈見季, 馬建鋒

    日本植物生理学会年会要旨集   48th   2007年

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  • オオムギのアルミニウム活性型クエン酸輸送体候補遺伝子の発現ならびに機能解析

    古川純, 山地直樹, 王華, 且原真木, 佐藤和広, 武田和義, 馬建鋒

    日本植物生理学会年会要旨集   48th   2007年

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  • イネアルミニウム耐性遺伝子Als1の機能解析

    黄朝鋒, 山地直樹, 矢野昌裕, 馬建鋒

    日本植物生理学会年会要旨集   48th   2007年

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  • イネ科NIPのケイ酸輸送特性の解析

    三谷奈見季, 山地直樹, 且原真木, 馬建鋒

    日本植物生理学会年会要旨集   48th   2007年

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  • 9-68 イネの根におけるケイ酸吸収関連遺伝子の分布と発現解析(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

    山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集   ( 52 )   73 - 73   2006年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • 9-36 オオムギの鉄-ムギネ酸錯体トランスポーター遺伝子の単離と解析(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

    馬 建鋒, 上野 大勢, 山地 直樹, 村田 佳子

    日本土壌肥料学会講演要旨集   ( 52 )   62 - 62   2006年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • イネのケイ酸吸収機構

    山地 直樹, 馬 建鋒

    化学と生物   44 ( 7 )   453 - 458   2006年7月

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    記述言語:日本語   出版者・発行元:Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI: 10.1271/kagakutoseibutsu1962.44.453

    CiNii Article

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  • イネのケイ酸輸送体.

    馬 建鋒, 山地直樹, 三谷奈見季, 玉井一規

    細胞工学   25:778-779   2006年

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  • 9-67 イネケイ酸吸収関連遺伝子lsi2の解析(9. 植物の無機栄養, 2006年度秋田大会講演要旨)

    玉井 一規, 山地 直樹, 三谷 奈見季, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集   52 ( 0 )   73 - 73   2006年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

    CiNii Article

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  • ケイ酸吸収遺伝子Lsi1の機能解析

    三谷奈見季, 山地直樹, 玉井一規, 小西左江子, 矢野昌裕, 馬建鋒

    日本植物生理学会年会要旨集   47th   2006年

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  • イネケイ酸吸収関連遺伝子Lsi2の機能解析

    山地直樹, 馬建鋒

    日本植物生理学会年会要旨集   47th   2006年

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  • 9-25 新規イネケイ酸吸収関連遺伝子Lsi2の単離と解析(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)

    玉井 一規, 三谷 奈見季, 山地 直樹, 小西 左江子, 矢野 昌裕, 馬 建鋒

    日本土壌肥料学会講演要旨集   51 ( 0 )   2005年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 9-26 イネケイ酸吸収関連遺伝子Lsi1の機能解析(9.植物の無機栄養,日本土壌肥料学会 2005年度大会講演要旨集)

    三谷 奈見季, 山地 直樹, 玉井 一規, 小西 左江子, 矢野 昌裕, 村田 佳子, 馬 建鋒

    日本土壌肥料学会講演要旨集   51 ( 0 )   66 - 66   2005年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 小形ワイヤ供給装置を使用した真空中でのステンレス鋼の宇宙GHTA溶接

    吹田 義一, 山地 直樹, 寺嶋 昇, 佃 芳行, 原 卓雄, 黄地 尚義, 増渕 興一

    日本機械学会論文集 C編   67 ( 658 )   2044 - 2050   2001年

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    記述言語:英語   出版者・発行元:The Japan Society of Mechanical Engineers  

    In this work, a feasibility study has been conducted to determine if GHTA (Gas Hollow Tungsten Arc) method can be used for the welding in space. As previously described, the test for GHTA welding of stainless steel without filler wire has been successfully performed in a simulated space environment, produced by the parabolic flight of aircraft. In this paper, melt-run welding tests and butt welding tests on stainless steels for GHTA welding with filler wire have been conducted in a vacuum chamber using a developed compact wire feeder. The results are summarized as follows. (1) The developed compact wire feeder makes it possible to add the filler metal to GHTA weld pool smoothly in a vacuum. (2) A metal vapor plume is formed on the molten pool in GHTA welding and it grows brightly with increase of arc current. (3) The penetration depth in GHTA welding becomes shallow with increase of filler metal, as well as that in GTA welding under the atmospheric pressure. (4) The butt joint welded by the GHTA method of filler wire addition has no defect and it has enough strength.

    DOI: 10.1299/kikaic.67.2044

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▼全件表示

講演・口頭発表等

  • 4-2-2 OsZIP2 mediates xylem loading and preferential distribution of Zn in rice(4-2 植物の微量栄養素 2022年度東京大会)

    黄 勝, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集  2022年9月5日  一般社団法人 日本土壌肥料学会

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    開催年月日: 2022年9月5日

    記述言語:日本語  

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  • 4-3-4 Duplication of a manganese/cadmium transporter gene leads to low cadmium accumulation in rice grain(4-3 植物の有害元素 2022年度東京大会)

    余 恩, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集  2022年9月5日  一般社団法人 日本土壌肥料学会

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    開催年月日: 2022年9月5日

    記述言語:日本語  

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  • 4-1-5 Identification of a gene involved in loading phosphorus into barley grains(4-1 植物の多量栄養素 2022年度東京大会)

    黄 衡亮, 久野 裕, 黄 勝, 三谷 奈見季, 佐藤 和広, 山地 直樹, 馬 建鋒

    日本土壌肥料学会講演要旨集  2022年9月5日  一般社団法人 日本土壌肥料学会

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    開催年月日: 2022年9月5日

    記述言語:日本語  

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  • P4-1-2 蛍光染色によるイネ葉のケイ素蓄積過程の解析(4-1 植物の多量栄養素 2022年度東京大会)

    山地 直樹, 藤井 理樹, 三谷 奈見季, 馬 建鋒

    日本土壌肥料学会講演要旨集  2022年9月5日  一般社団法人 日本土壌肥料学会

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    開催年月日: 2022年9月5日

    記述言語:日本語  

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  • オオムギ根内生微生物の同定と役割の解明

    木代勝元, 最相大輔, 山下純, 山地直樹, 山本敏央, 門田有希, 持田恵一, 中川智行, 谷明生

    日本農芸化学会大会講演要旨集(Web)  2022年 

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    開催年月日: 2022年

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  • 節のミネラル分配を理解してコメの栄養価を高める.

    山地直樹

    埼玉大学・岡山大学 若手合同シンポジウム 

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    開催年月日: 2020年11月26日

    記述言語:日本語   会議種別:口頭発表(一般)  

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  • イネのマンガン欠乏感受性に関与する輸送体.

    上野大勢・古田富大・三宅親弘・車 景・山地直樹・馬 建鋒・岩崎貢三

    日本土壌肥料学会2020年度岡山大会 

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    開催年月日: 2020年9月8日 - 2020年9月10日

    記述言語:日本語   会議種別:口頭発表(一般)  

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  • レーザーアブレーション-ICP-MSによる植物組織の元素イメージング.多元素/安定同位体の定性/定量イメージング.

    山地直樹

    日本土壌肥料学会2020年度岡山大会シンポジウムI 

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    開催年月日: 2020年9月8日 - 2020年9月10日

    記述言語:日本語   会議種別:口頭発表(一般)  

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  • Identification of a transporter responsible for Zn uptake in rice.

    黄 勝・佐々木明正・山地直樹・三谷奈見季・馬 建鋒

    日本土壌肥料学会2020年度岡山大会 

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    開催年月日: 2020年9月8日 - 2020年9月10日

    記述言語:英語   会議種別:口頭発表(一般)  

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  • ケイ酸輸送体Lsi2の相同遺伝子SIET3,4,5の機能解析.

    三谷奈見季・山地直樹・馬 建鋒

    日本土壌肥料学会2020年度岡山大会 

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    開催年月日: 2020年9月8日 - 2020年9月10日

    記述言語:日本語   会議種別:口頭発表(一般)  

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  • ソバのマンガン高集積に関与するFeNramp5の機能解析.

    横正健剛・山地直樹・馬 建鋒

    日本土壌肥料学会2020年度岡山大会 

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    開催年月日: 2020年9月8日 - 2020年9月10日

    記述言語:日本語   会議種別:口頭発表(一般)  

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  • Functional characterization of OsCASP1 involved in formation of Casparian strip in rice.

    Huang, S., Yamaji, N., Xia, J. X. and Ma, J. F.

    第61回日本植物生理学会年会 

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    開催年月日: 2020年3月19日 - 2020年3月21日

    記述言語:英語   会議種別:口頭発表(一般)  

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  • Identification of a transporter for preferential distribution of phosphorus in Arabidopsis.

    Ding, G., Lei, G. J., Yamaji, N., Yokosho, K., Mitani-Ueno, N., Huang, S. and Ma, J. F.

    第61回日本植物生理学会年会 

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    開催年月日: 2020年3月19日 - 2020年3月21日

    記述言語:日本語   会議種別:口頭発表(一般)  

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  • Mechanism underlying differential expression of HvHMA3 involved in cadmium accumulation in barley.

    Lei, G. J., Hisano, H., Saisho, D., Yamaji, N., Sato, K. and Ma, J. F.

    第61回日本植物生理学会年会 

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    開催年月日: 2020年3月19日 - 2020年3月21日

    記述言語:英語   会議種別:口頭発表(一般)  

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  • Mutation of OsVIT2 alters iron distribution in rice.

    Che, J., Yamaji, N. and Ma, J. F.

    第61回日本植物生理学会年会 

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    開催年月日: 2020年3月19日 - 2020年3月21日

    記述言語:英語   会議種別:口頭発表(一般)  

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  • イネ細胞膜H+-ATPase の能動的なSi 取り込みへの関与.

    Yamamoto, S., Kawai, Y., Okumura, M., You, T., Mitani-Ueno, N., Yamaji, N., Ma, J. F. and Kinoshita, T.

    第61回日本植物生理学会年会 

     詳細を見る

    開催年月日: 2020年3月19日 - 2020年3月21日

    記述言語:日本語   会議種別:口頭発表(一般)  

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  • Role of casparian strip in uptake of mineral elements in rice.

    Huang, S., Yamaji, N., Xia, J. and Ma, J. F.

    2019年度日本土壌肥料学会関西支部講演会 

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    開催年月日: 2019年12月5日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:鳥取  

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  • Altered root structure affects both expression and cellular localization of transporters for mineral element uptake in rice.

    Yu, E., Yamaji, N. and Ma, J. F.

    The 17th International Symposium on Rice Functional Genomics 

     詳細を見る

    開催年月日: 2019年11月4日 - 2019年11月6日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Taipei, Taiwan  

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  • Functional characterization of OsBBPls, a putative ART1-interactive protein in rice.

    Yokosho, K., Chen, Z. C., Yamaji, N. and Ma, J. F.

    The 17th International Symposium on Rice Functional Genomics 

     詳細を見る

    開催年月日: 2019年11月4日 - 2019年11月6日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Taipei, Taiwan  

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  • Casparian strip in rice roots play an important role in selective uptake of mineral elements.

    Huang, S., Yamaji, N., Xia, J. and Ma, J. F.

    The 17th International Symposium on Rice Functional Genomics 

     詳細を見る

    開催年月日: 2019年11月4日 - 2019年11月6日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Taipei, Taiwan  

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  • Functional characterization of two metallothionein genes highly expressed in rice nodes.

    Lei, G. J., Yamaji, N. and Ma, J. F.

    The 17th International Symposium on Rice Functional Genomics 

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    開催年月日: 2019年11月4日 - 2019年11月6日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Taipei, Taiwan  

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  • OsOPT7 is involved in Fe distribution.

    Yamaji, N., Yokosho, K., Sasaki, A. and Ma, J. F.

    The 17th International Symposium on Rice Functional Genomics 

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    開催年月日: 2019年11月4日 - 2019年11月6日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Taipei, Taiwan  

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  • イネの鉄分配に関与するOsOPT7の詳細解析.

    山地直樹・横正健剛・佐々木明正・馬 建鋒

    日本土壌肥料学会2019年度静岡大会 

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    開催年月日: 2019年9月3日 - 2019年9月5日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:静岡  

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  • イネのLsi2相同遺伝子の機能解析.

    三谷奈見季・山地直樹・馬 建鋒

    日本土壌肥料学会2019年度静岡大会 

     詳細を見る

    開催年月日: 2019年9月3日 - 2019年9月5日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:静岡  

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  • Global transcriptomic analysis on Si-improved mineral balance in rice.

    黄 勝・山地直樹・馬 建鋒

    日本土壌肥料学会2019年度静岡大会 

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    開催年月日: 2019年9月3日 - 2019年9月5日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:静岡  

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  • イネにおけるケイ素輸送モデルによる輸送体配置の影響分析.

    櫻井 玄・山地直樹・三谷奈見季・小野圭介・横沢正幸・酒井英光・吉本真由美・馬 建鋒

    日本土壌肥料学会2019年度静岡大会 

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    開催年月日: 2019年9月3日 - 2019年9月5日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:静岡  

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  • イネにおけるホウ素の優先的分配に関わる輸送体の更なる解析.

    邵 継鋒・山地直樹・黄 勝・馬 建鋒

    日本土壌肥料学会2019年度静岡大会 

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    開催年月日: 2019年9月3日 - 2019年9月5日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:静岡  

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  • Functional analysis of a QTL gene controlling Cd accumulation in barley.

    雷 貴傑・久野 裕・山地直樹・佐藤和広・馬 建鋒

    日本土壌肥料学会2019年度静岡大会 

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    開催年月日: 2019年9月3日 - 2019年9月5日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:静岡  

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  • OsPHO1;2 is required for P allocation to the rice grain.

    Che, J., Yamaji, N., Mitani-Ueno, N., Miyaji, T. and Ma, J. F.

    Gordon Research Conference on Organellar Channels and Transporters 2019 

     詳細を見る

    開催年月日: 2019年8月4日 - 2019年8月9日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Mount Snow, USA  

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  • Zinc transporters for uptake, translocation and distribution in rice.

    Ma, J. F., Yamaji, N. and Sasaki, A.

    The 15th International Conference on the Biogeochemistry of Trace Elements 

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    開催年月日: 2019年5月5日 - 2019年5月9日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Nanjing, China  

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  • 作物の持続的な生産に向けた植物栄養からのアプローチ.

    馬 建鋒・山地直樹

    日本農芸化学会2019年度東京大会 

     詳細を見る

    開催年月日: 2019年3月24日 - 2019年3月27日

    記述言語:日本語   会議種別:口頭発表(招待・特別)  

    開催地:東京  

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  • Node-based transporters for preferential distribution of mineral elements.

    Yamaji, N. and Ma, J. F.

    第60回日本植物生理学会年会 

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    開催年月日: 2019年3月13日 - 2019年3月15日

    記述言語:英語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:名古屋  

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  • 植物ケイ素輸送体の構造.

    Saitoh, Y., Matsuki, K., Yonekura, S., Yang, L., Mitani-Ueno, N., Yamaji, N., Shen, J., Ma, J. F. and Suga, M.

    第60回日本植物生理学会年会 

     詳細を見る

    開催年月日: 2019年3月13日 - 2019年3月15日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • イネにおけるケイ素のミクロスケールモデルとマクロスケールモデルの統合.

    Sakurai, G., Yamaji, N., Mitani-Ueno, N., Yokozawa, M., Ono, K. and Ma, J. F.

    第60回日本植物生理学会年会 

     詳細を見る

    開催年月日: 2019年3月13日 - 2019年3月15日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • Functional analysis of a node-expressed transporter for phytosiderophore in rice.

    Che, J., Yokosyo, K., Yamaji, N. and Ma, J. F.

    第60回日本植物生理学会年会 

     詳細を見る

    開催年月日: 2019年3月13日 - 2019年3月15日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • イネのアルミニウム耐性転写因子ART1 と相互作用するタンパク質OsBBPIsの機能解析.

    Yokosho, K., Chen, Z. C., Yamaji, N. and Ma, J. F.

    第60回日本植物生理学会年会 

     詳細を見る

    開催年月日: 2019年3月13日 - 2019年3月15日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • イネのリン再転流関連遺伝子の同定と機能解析.

    Mitani-Ueno, N., Yamaji, N. and Ma, J. F.

    第60回日本植物生理学会年会 

     詳細を見る

    開催年月日: 2019年3月13日 - 2019年3月15日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:名古屋  

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  • Different roles of two variants of a half-size ABC transporter in Al accumulation and detoxification of buckwheat.

    Lei, G. J., Yokosho, K., Yamaji, N., Fujii-Kashino, M. and Ma, J. F.

    第60回日本植物生理学会年会 

     詳細を見る

    開催年月日: 2019年3月13日 - 2019年3月15日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • Reducing accumulation of toxic metal and metalloid in rice grain.

    Ma, J. F., Song, W. Y., Zhao, F., Shao, J. F. and Yamaji, N.

    16th International Symposium on Rice Functional Genomics 

     詳細を見る

    開催年月日: 2018年9月5日 - 2018年9月7日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Tokyo, Japan  

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  • イネのART1と相互作用するタンパク質OsBBPIsの機能解析.

    横正健剛・陳 志長・山地直樹・馬 建鋒

    日本土壌肥料学会2018年度神奈川大会 

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    開催年月日: 2018年8月29日 - 2018年8月31日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:神奈川  

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  • Functional characterization of two metallothionein genes highly expressed in rice nodes.

    Lei, G., Yamaji, N. and Ma, J. F.

    日本土壌肥料学会2018年度神奈川大会 

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    開催年月日: 2018年8月29日 - 2018年8月31日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:神奈川  

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  • レーザーアブレーションICP-MSを用いたイネ節の維管束間輸送過程の解析.

    山地直樹・馬 建鋒

    日本土壌肥料学会2018年度神奈川大会 

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    開催年月日: 2018年8月29日 - 2018年8月31日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:神奈川  

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  • イネにおけるホウ素の優先的分配に関与する輸送体.

    邵 継鋒・山地直樹・馬 建鋒

    日本土壌肥料学会2018年度神奈川大会 

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    開催年月日: 2018年8月29日 - 2018年8月31日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:神奈川  

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  • イネの節における無機栄養素の維管束間輸送とその可視化の試み

    山地直樹

    第13回トランスポーター研究会年会 

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    開催年月日: 2018年7月21日 - 2018年7月22日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡  

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  • An Al-inducible transcription factor, ART2 is involved in Al tolerance in rice.

    Che, J., Tsutsui, T., Yokosho, K., Yamaji, N. and Ma, J. F.

    10th International Symposium on Plant-Soil Interactions at Low pH 

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    開催年月日: 2018年6月25日 - 2018年6月28日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Putrajaya, Malaysia  

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  • Functional characterization of OsBBBPI3, a putative ART1-interactive protein in rice.

    Yokosho, K., Chen, Z. C., Yamaji, N. and Ma, J. F.

    10th International Symposium on Plant-Soil Interactions at Low pH 

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    開催年月日: 2018年6月25日 - 2018年6月28日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Putrajaya, Malaysia  

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  • Regulation of aluminum tolerance in barley.

    Ma, J. F., Fujii-Kashino, M., Yamaji, N., Yamane, M., Saisho, D. and Sato, K.

    10th International Symposium on Plant-Soil Interactions at Low pH 

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    開催年月日: 2018年6月25日 - 2018年6月28日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Putrajaya, Malaysia  

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  • Transporters involved in preferential distribution of boron in rice.

    Shao, J. F., Yamaji, N., Shen, R. F. and Ma, J. F.

    10th International Symposium on Plant-Soil Interactions at Low pH 

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    開催年月日: 2018年6月25日 - 2018年6月28日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Putrajaya, Malaysia  

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  • An alternative splicing transcript of FeALS1.1 is implicated in Al detoxification in buckwheat.

    Lei, G. J., Yokosho, K., Yamaji, N., Fujii-Kashino, M. and Ma, J. F.

    10th International Symposium on Plant-Soil Interactions at Low pH 

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    開催年月日: 2018年6月25日 - 2018年6月28日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Putrajaya, Malaysia  

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  • レーザーアブレーションICP-MSによるイネ節の元素分布イメージング.

    Yamaji, N. and Ma, J. F.

    第59回日本植物生理学会年会 

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    開催年月日: 2018年3月28日 - 2018年3月30日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:札幌  

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  • Functional characterization of a transcription factor (ART2) implicated in aluminum tolerance of rice.

    Che, J., Tsutsui, T., Yokosho, K., Yamaji, N. and Ma, J. F.

    第59回日本植物生理学会年会 

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    開催年月日: 2018年3月28日 - 2018年3月30日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

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  • Transporters involved in preferential distribution of boron in rice.

    Shao, J. F., Yamaji, N. and Ma, J. F.

    第59回日本植物生理学会年会 

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    開催年月日: 2018年3月28日 - 2018年3月30日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

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  • Fine mapping of a QTL gene for Cd accumulation in barley.

    Lei, G., Kashino, M., Wu, D., Yamaji, N., Sato, K. and Ma, J. F.

    第59回日本植物生理学会年会 

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    開催年月日: 2018年3月28日 - 2018年3月30日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

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  • Molecular mechanisms for preferential distribution of boron in rice.

    Shao, J. F., Yamaji, N. and Ma, J. F.

    2017年度日本土壌肥料学会関西支部会  2017年 

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    開催年月日: 2017年12月7日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:橿原, 奈良  

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  • トランスゴルジ局在型MTP11はイネのマンガン耐性に関与する

    常光優太・宮崎 彰・山地直樹・馬 建鋒・加藤伸一郎・岩崎貢三・上野大勢

    2017年度日本土壌肥料学会関西支部会  2017年 

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    開催年月日: 2017年12月7日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:橿原, 奈良  

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  • A cooperative transport system for silicon in plants.

    Yamaji, N., Mitani-Ueno, N., Sakurai, G. and Ma, J.F.

    7th International Conference on Silicon in Agriculture  2017年 

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    開催年月日: 2017年10月24日 - 2017年10月28日

    記述言語:英語   会議種別:口頭発表(基調)  

    開催地:Bengaluru, India  

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  • イネの"節"の構造と機能

    山地直樹

    2017年秋期日本育種学会  2017年 

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    開催年月日: 2017年10月6日 - 2017年10月8日

    記述言語:日本語   会議種別:口頭発表(招待・特別)  

    開催地:盛岡  

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  • ソバのNrampファミリー遺伝子の機能解析

    横正健剛・邵 継鋒・山地直樹・馬 建鋒

    日本土壌肥料学会2017年度仙台大会  2017年 

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    開催年月日: 2017年9月5日 - 2017年9月7日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台  

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  • イネのケイ酸輸送体Lsi6の節における著しい高発現は組換えプロモーターでは再現できない

    山地直樹・馬 建鋒

    日本土壌肥料学会2017年度仙台大会  2017年 

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    開催年月日: 2017年9月5日 - 2017年9月7日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:仙台  

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  • Functional characterization of genes involved in P distribution in rice node.

    Che, J., Yamaji, N. and Ma, J. F.

    日本土壌肥料学会2017年度仙台大会  2017年 

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    開催年月日: 2017年9月5日 - 2017年9月7日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:仙台  

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  • オオムギの新規アルミニウム耐性制御機構

    馬 建鋒・柏野美帆・山地直樹

    日本土壌肥料学会2017年度仙台大会  2017年 

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    開催年月日: 2017年9月5日 - 2017年9月7日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台  

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  • Uptake, distribution and detoxification of manganese in rice.

    Ma, J. F., Yamaji, N., Sasaki, A. and Ueno, D.

    XVIII International Plant Nutrition Colloquium 2017, Manganese satellite meeting  2017年 

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    開催年月日: 2017年8月19日 - 2017年8月24日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Copenhagen, Denmark  

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  • Molecular mechanisms for distribution of mineral elements in plants.

    Ma, J. F. and Yamaji, N.

    XVIII International Plant Nutrition Colloquium 2017  2017年 

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    開催年月日: 2017年8月19日 - 2017年8月24日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Copenhagen, Denmark  

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  • A member of MFS is involved in phytosiderophore efflux for metal distribution in rice.

    Che, J., Yokosho, K., Yamaji, N. and Ma, J. F.

    XVIII International Plant Nutrition Colloquium 2017  2017年 

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    開催年月日: 2017年8月19日 - 2017年8月24日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Copenhagen, Denmark  

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  • Involvement of two MATE transporters with different subcellular localizations in buckwheat Al tolerance.

    Lei, G. J., Yokosho, K., Yamaji, N. and Ma, J. F.

    XVIII International Plant Nutrition Colloquium 2017  2017年 

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    開催年月日: 2017年8月19日 - 2017年8月24日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Copenhagen, Denmark  

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  • Functional characterization of Nramp transporter genes in buckwheat.

    Yokosho, K., Shao, J. F., Yamaji, N., Shen, R. F. and Ma, J. F.

    XVIII International Plant Nutrition Colloquium 2017  2017年 

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    開催年月日: 2017年8月19日 - 2017年8月24日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Copenhagen, Denmark  

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  • A novel phosphate transporter controlling grain P accumulation in rice.

    Yamaji, N., Takemoto, Y., Miyaji, T., Mitani-Ueno, N., Yoshida, K. T. and Ma, J. F.

    XVIII International Plant Nutrition Colloquium 2017  2017年 

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    開催年月日: 2017年8月19日 - 2017年8月24日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Copenhagen, Denmark  

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  • Preferential distribution of boron to developing tissues is mediated by OsNIP3;1 localized in rice node.

    Shao, J. F., Yamaji, N., Shen, R. F. and Ma, J. F.

    XVIII International Plant Nutrition Colloquium 2017  2017年 

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    開催年月日: 2017年8月19日 - 2017年8月24日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Copenhagen, Denmark  

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  • Preferential distribution of boron to developing tissues is mediated by OsNIP3;1 localized in rice node.

    Shao, J. F., Yamaji, N. and Ma, J. F.

    第12回トランスポーター研究会年会  2017年 

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    開催年月日: 2017年7月8日 - 2017年7月9日

    記述言語:英語   会議種別:ポスター発表  

    開催地:仙台  

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  • Implication of two genes encoding a bacterial-type ATP-binding cassette transporter in Al tolerance in buckwheat.

    Che, J., Yokosho, K., Yamaji, N. and Ma, J. F.

    第12回トランスポーター研究会年会  2017年 

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    開催年月日: 2017年7月8日 - 2017年7月9日

    記述言語:英語   会議種別:ポスター発表  

    開催地:仙台  

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  • Two MATE transporters with different subcellular localization are involved in Al tolerance in buckwheat.

    Lei, G. J., Yokosho, K., Yamaji, N. and Ma, J. F.

    第12回トランスポーター研究会年会  2017年 

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    開催年月日: 2017年7月8日 - 2017年7月9日

    記述言語:英語   会議種別:ポスター発表  

    開催地:仙台  

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  • コメのミネラル蓄積を左右する節の輸送制御.シンポジウム 『いま注目のトランスポーター研究?』

    山地直樹

    第12回トランスポーター研究会年会  2017年 

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    開催年月日: 2017年7月8日 - 2017年7月9日

    記述言語:日本語   会議種別:口頭発表(招待・特別)  

    開催地:仙台  

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  • ソバのNrampファミリー遺伝子の機能解析

    横正健剛・邵 継鋒・山地直樹・沈 仁芳・馬 建鋒

    第58回日本植物生理学会年会  2017年 

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    開催年月日: 2017年3月16日 - 2017年3月18日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:鹿児島  

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  • Role of two genes encoding metallothionein in distribution of metals in rice.

    Lei, G. J., Yamaji, N. and Ma, J. F.

    第58回日本植物生理学会年会  2017年 

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    開催年月日: 2017年3月16日 - 2017年3月18日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:鹿児島  

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  • イネ・オオムギ・ライムギの根圏pH応答性の比較トランスクリプトーム解析

    山地直樹・柏野(藤井)美帆・横正健剛・馬 建鋒

    第58回日本植物生理学会年会  2017年 

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    開催年月日: 2017年3月16日 - 2017年3月18日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:鹿児島  

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  • OsPHO1;2 is also involved in intervascular transfer of phosphorus in rice node.

    Che, J., Yamaji, N. and Ma, J. F.

    第58回日本植物生理学会年会  2017年 

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    開催年月日: 2017年3月16日 - 2017年3月18日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:鹿児島  

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  • Novel phosphate transporter in rice node and grain P control

    Yamaji, N.

    33rd IPSR International Symposium and 9th Symposium on Plant Stres Sciences.  2017年 

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    開催年月日: 2017年3月6日 - 2017年3月7日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Kurashiki  

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  • Reducing phosphorus accumulation in rice grains with an impaired transporter in the node.

    Yamaji, N.

    International Workshop on Soil-Microbe-Plant Interaction  2016年 

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    開催年月日: 2016年12月3日 - 2016年12月4日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Kurashiki, Japan  

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  • Two genes encoding bacterial-type ABC transporters are required for Al tolerance in buckwheat.

    車 景・横正健剛・山地直樹・馬 建鋒

    日本土壌肥料学会2016年度大会  2016年 

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    開催年月日: 2016年9月20日 - 2016年9月22日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:佐賀  

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  • イネ由来ケイ酸輸送体Lsi1の更なる解析.

    三谷奈見季・山地直樹・馬 建鋒

    日本土壌肥料学会2016年度大会  2016年 

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    開催年月日: 2016年9月20日 - 2016年9月22日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:佐賀  

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  • Further characterization of two Al-inducible MATE genes in buckwheat.

    雷 貴傑・横正健剛・山地直樹・馬 建鋒

    日本土壌肥料学会2016年度大会  2016年 

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    開催年月日: 2016年9月20日 - 2016年9月22日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:佐賀  

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  • イネ科作物の土壌pH適応におけるトレードオフの可能性.

    山地直樹・柏野美帆・横正健剛・馬 建鋒

    日本土壌肥料学会2016年度大会  2016年 

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    開催年月日: 2016年9月20日 - 2016年9月22日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:佐賀  

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  • An Al-inducible expansin gene, OsEXPA10 is involved in root elongation of rice.

    Che, J., Yamaji, N. and Ma, J. F.

    Plant Biology 2016  2016年 

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    開催年月日: 2016年7月9日 - 2016年7月13日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Austin, Texas, USA  

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  • Isolation and characterization of a rice line with high Cd accumulation.

    Shao, J. F., Kashino, M., Yamaji, N. and Ma, J. F.

    Plant Biology 2016  2016年 

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    開催年月日: 2016年7月9日 - 2016年7月13日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Austin, Texas, USA  

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  • Two tonoplast-localized half-size ABC transporters are involved in internal detoxification of Al in buckwheat.

    Lei, G., Yokosho, K., Yamaji, N., Kashino, M. and Ma, J. F.

    Plant Biology 2016  2016年 

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    開催年月日: 2016年7月9日 - 2016年7月13日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Austin, Texas, USA  

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  • イネ節の新規リン酸輸送体と少燐米の可能性

    山地直樹・竹本侑馬・宮地孝明・三谷奈見季・吉田薫・馬建鋒

    イネ遺伝学・分子生物学ワークショップ2016  2016年 

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    開催年月日: 2016年7月4日 - 2016年7月5日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • イネの品種間におけるクエン酸トランスポーターOsFRDL4の発現とその制御機構

    横正健剛・山地直樹・馬 建鋒

    イネ遺伝学・分子生物学ワークショップ2016  2016年 

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    開催年月日: 2016年7月4日 - 2016年7月5日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • コメへの優先的リン分配を担うイネの新規リン酸輸送体.

    山地直樹・竹本侑馬・宮地孝明・三谷奈見季・吉田 薫・馬 建鋒

    第11回トランスポーター研究会年会,第316回生物圏シンポジウム  2016年 

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    開催年月日: 2016年7月2日 - 2016年7月3日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:京都  

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  • OsSultr3;4 localized in rice node is responsible for preferential distribution of Pi to new leaves and grains.

    Yamaji, N., Takemoto, Y., Mitani-Ueno, N. and Ma, J. F.

    The 17th International Workshop on Plant Membrane Biology (IWPMB2016)  2016年 

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    開催年月日: 2016年6月5日 - 2016年6月10日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Annapolis, Maryland, USA  

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  • Transporters for uptake, distribution and detoxification of Mn in rice.

    Ma, J. F., Yamaji, N., Sasaki, A. and Ueno, D.

    The 17th International Workshop on Plant Membrane Biology (IWPMB2016)  2016年 

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    開催年月日: 2016年6月5日 - 2016年6月10日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Annapolis, Maryland, USA  

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  • イネ節に局在するOsSultr3;4は新葉や穀粒へのリンの優先的分配に関与する.

    竹本侑馬・山地直樹・馬 建鋒

    第57回日本植物生理学会年会  2016年 

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    開催年月日: 2016年3月18日 - 2016年3月20日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:盛岡  

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  • オオムギアルミニウム耐性遺伝子HvAACT1の新規発現調節機構の解析.

    柏野(藤井)美帆・山地直樹・山根美樹・最相大輔・佐藤和広・馬 建鋒

    第57回日本植物生理学会年会  2016年 

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    開催年月日: 2016年3月18日 - 2016年3月20日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:盛岡  

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  • イネの1番染色体のAl耐性QTLはOsFRDL4の発現の違いに由来する.

    横正健剛・山地直樹・馬 建鋒

    第57回日本植物生理学会年会  2016年 

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    開催年月日: 2016年3月18日 - 2016年3月20日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:盛岡  

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  • OsSultr3;4 expressed at the node is involved in phosphorus distribution in rice.

    Takemoto, Y., Yamaji, N. and Ma, J. F.

    9th International Symposium on Plant-Soil Interactions at Low pH.  2015年 

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    開催年月日: 2015年10月18日 - 2015年10月23日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Dubrovnik, Croatia  

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  • Possibility of trade-off between acidic and alkaline soil adaptation in graminaceous plants.

    Yamaji, N., Kashino-Fujii, M., Yokosho, K. and Ma, J. F.

    9th International Symposium on Plant-Soil Interactions at Low pH.  2015年 

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    開催年月日: 2015年10月18日 - 2015年10月23日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Dubrovnik, Croatia  

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  • Transport and detoxification of manganese in rice.

    Ma, J. F., Sasaki, A., Ueno, D. and Yamaji, N.

    9th International Symposium on Plant-Soil Interactions at Low pH.  2015年 

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    開催年月日: 2015年10月18日 - 2015年10月23日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Dubrovnik, Croatia  

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  • A co-operated transport system for mineral element uptake in rice roots.

    Ma, J. F., Yamaji, N., Sasaki, A., Mitani-Ueno, N. and Ueno, D.

    International Society of Root Research 9th Symposium.  2015年 

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    開催年月日: 2015年10月6日 - 2015年10月9日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Canberra, Australia  

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  • Molecular mechanisms of cadmium accumulation in rice.

    Ma, J. F., Sasaki, A., Ueno, D. and Yamaji, N.

    13th International Symposium on Rice Functional Genomics.  2015年 

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    開催年月日: 2015年9月21日 - 2015年9月24日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Wuhan, China  

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  • イネ節で高発現する輸送体遺伝子OsSultr3;4の機能解析.

    竹本侑馬・山地直樹・馬 建鋒

    日本土壌肥料学会2015年度京都大会  2015年 

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    開催年月日: 2015年9月9日 - 2015年9月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都  

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  • ヒ素輸送体遺伝子OsABCC1の発現量の品種間差解析.

    馬 建鋒・山地直樹・山木智央

    日本土壌肥料学会2015年度京都大会  2015年 

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    開催年月日: 2015年9月9日 - 2015年9月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都  

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  • イネ節におけるケイ酸分配機構の統合解析.

    山地直樹・櫻井 玄・三谷奈見季・馬 建鋒

    日本土壌肥料学会2015年度京都大会  2015年 

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    開催年月日: 2015年9月9日 - 2015年9月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都  

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  • Characterization of two Al-induced half-size ABC transporters, FeALS1 with different size in buckwheat.

    雷 貴傑・横正健剛・山地直樹・柏野美帆・馬 建鋒

    日本土壌肥料学会2015年度京都大会  2015年 

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    開催年月日: 2015年9月9日 - 2015年9月11日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:京都  

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  • Functional characterization of an Al-inducible expansin gene, OsEXPA10 in rice.

    車 景・山地直樹・馬 建鋒

    日本土壌肥料学会2015年度京都大会  2015年 

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    開催年月日: 2015年9月9日 - 2015年9月11日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:京都  

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  • アルミニウム処理下のソバの細胞膜と液胞膜のプロテオーム解析.

    横正健剛・雷 貴傑・山地直樹・馬 建鋒

    日本土壌肥料学会2015年度京都大会  2015年 

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    開催年月日: 2015年9月9日 - 2015年9月11日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:京都  

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  • Strategy for developing rice with reduced As accumulation in grain.

    Song, W-Y., Yamaki, T., Yamaji, N., Ko, D., Fujii, M., Jung, K-H., An, G., Martinoia, E., Ma, J. F. and Lee, Y.

    13th International Conference on the Biogeochemistry of Trace Elements.  2015年 

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    開催年月日: 2015年7月12日 - 2015年7月16日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Fukuoka, Japan  

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  • Detection of novel QTLs controlling Cd accumulation in rice.

    Deng, F., Yamaki, T., Shomura, A., Ando, T., Fukuoka, S. and Ma, J. F.

    13th International Conference on the Biogeochemistry of Trace Elements.  2015年 

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    開催年月日: 2015年7月12日 - 2015年7月16日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Fukuoka, Japan  

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  • Molecular mechanisms of cadmium accumulation in cereal crops.

    Ma, J. F., Yamaji, N., Sasaki, A., Ueno, D. and Wu, D.

    13th International Conference on the Biogeochemistry of Trace Elements.  2015年 

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    開催年月日: 2015年7月12日 - 2015年7月16日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Fukuoka, Japan  

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  • Vacuolar sequestration of Mn in leaf epidermis by OsMTP8 is required for Mn tolerance in rice.

    Ueno, D., Takemoto, Y., Kotake, S., Yamaji, N., Ma, J. F., Kato, S. and Iwasaki, K.

    13th International Conference on the Biogeochemistry of Trace Elements.  2015年 

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    開催年月日: 2015年7月12日 - 2015年7月16日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Fukuoka, Japan  

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  • イネのリン分配に関与する輸送体OsSultr3;4の機能解析.

    竹本侑馬・山地直樹・馬 建鋒

    第56回日本植物生理学会年会  2015年 

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    開催年月日: 2015年3月16日 - 2015年3月18日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:東京  

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  • AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis.

    Miyaji, T., Kuromori, T., Takeuchi, Y., Yamaji, N., Yokosho, K., Shimazawa, A., Sugimoto, E., Omote, H., Ma, J. F., Shinozaki, K. and Moriyama, Y.

    第56回日本植物生理学会年会  2015年 

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    開催年月日: 2015年3月16日 - 2015年3月18日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • Cadmium uptake is mediated by a manganese transporter, HvNramp5 in barley.

    Wu, D., Yamaji, N., Yamane, M., Kashino, M., Sato, K. and Ma, J. F.

    第56回日本植物生理学会年会  2015年 

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    開催年月日: 2015年3月16日 - 2015年3月18日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • Further characterization of OsABCC1 involved in As accumulation in rice.

    Song, W-Y., Yamaki, T., Yamaji, N., Lee, Y. and Ma, J. F.

    第56回日本植物生理学会年会  2015年 

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    開催年月日: 2015年3月16日 - 2015年3月18日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • 亜鉛欠乏時に根で高発現するイネOsZIP9の機能解析.

    佐々木明正・柏野美帆・山地直樹・馬 建鋒

    第56回日本植物生理学会年会  2015年 

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    開催年月日: 2015年3月16日 - 2015年3月18日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:東京  

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  • Functional characterization of a cation transporter required for root growth in rice.

    Chen, Z., Yamaji, N. and Ma, J. F.

    第56回日本植物生理学会年会  2015年 

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    開催年月日: 2015年3月16日 - 2015年3月18日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • Functional characterization of plasma membrane H+-ATPase of rice.

    Toda, Y., Wang, Y., Takahashi, A., Yamaji, N., Ma, J. F., Ashikari, M. and Kinoshita, T.

    第56回日本植物生理学会年会  2015年 

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    開催年月日: 2015年3月16日 - 2015年3月18日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • イネのヒ素耐性と集積に関与する輸送体ABCC1の機能解析.

    山木智央・山地直樹・Won Yong Song・Youngsook Lee・馬 建鋒

    日本土壌肥料学会関西支部講演会  2014年 

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    開催年月日: 2014年12月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:高松  

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  • イネのマンガン耐性におけるOsMTP8の生理的意義.

    上野大勢・小竹咲也子・竹本侑馬・山地直樹・馬 建鋒・加藤伸一郎・岩崎貢三

    日本土壌肥料学会関西支部講演会  2014年 

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    開催年月日: 2014年12月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:高松  

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  • イネにおけるリンの分配に関与する輸送体OsSultr3;4の解析.

    竹本侑馬・山地直樹・馬 建鋒

    日本土壌肥料学会関西支部講演会  2014年 

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    開催年月日: 2014年12月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:高松  

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  • Molecular mechanisms of mineral element distribution in rice.

    Ma, J. F., Yamaji, N. and Sasaki, A.

    12th International Symposium on Rice Functional Genomics.  2014年 

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    開催年月日: 2014年11月16日 - 2014年11月19日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Arizona, USA  

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  • Characterization of a gene controlling root growth in rice.

    Chen, Z. C., Yamaji, N. and Ma, J. F.

    12th International Symposium on Rice Functional Genomics.  2014年 

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    開催年月日: 2014年11月16日 - 2014年11月19日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Arizona, USA  

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  • 節とミネラル分配

    山地直樹

    岡山大学資源植物科学研究所創立100周年記念シンポジウム  2014年 

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    開催年月日: 2014年10月3日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:倉敷  

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  • Dissection of Si-decreased Mn accumulation in rice shoots.

    車 景・山地直樹・馬 建鋒

    日本土壌肥料学会2014年度東京大会  2014年 

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    開催年月日: 2014年9月9日 - 2014年9月11日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • イネヒ素集積のQTL解析.

    馬 建鋒・山地直樹・竹本侑馬・福岡修一・正村純彦・安藤 露

    日本土壌肥料学会2014年度東京大会  2014年 

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    開催年月日: 2014年9月9日 - 2014年9月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • アルミニウム集積植物ソバのAl応答遺伝子の網羅的発現解析.

    横正健剛・山地直樹・馬 建鋒

    日本土壌肥料学会2014年度東京大会  2014年 

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    開催年月日: 2014年9月9日 - 2014年9月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • 環境変動に対する輸送体の応答機構.

    山地直樹・馬 建鋒

    日本土壌肥料学会2014年度東京大会  2014年 

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    開催年月日: 2014年9月9日 - 2014年9月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • イネの鉄分配に関与するOsOPT7の解析.

    山地直樹・佐々木明正・柏野美帆・馬 建鋒

    日本土壌肥料学会2014年度東京大会  2014年 

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    開催年月日: 2014年9月9日 - 2014年9月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • A possible chloride transporter required for root growth in rice.

    陳 志長・山地直樹・馬 建鋒

    日本土壌肥料学会2014年度東京大会  2014年 

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    開催年月日: 2014年9月9日 - 2014年9月11日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • イネにおけるケイ素吸収・輸送モデルによる植物体構造とその意義の解明.

    櫻井 玄・佐竹暁子・山地直樹・三谷奈見季・横沢正幸・馬 建鋒

    日本土壌肥料学会2014年度東京大会  2014年 

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    開催年月日: 2014年9月9日 - 2014年9月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • Silicon transporters and their role in plants.

    Ma, J. F., Yamaji, N. and Mitani-Ueno, N.

    6th International Conference on Silicon in Agriculture.  2014年 

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    開催年月日: 2014年8月26日 - 2014年8月30日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Stockholm, Sweden  

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  • Transcriptional regulation of Si transporter genes involved in uptake and distribution in rice.

    Yamaji, N., Mitani-Ueno, N. and Ma, J. F.

    6th International Conference on Silicon in Agriculture.  2014年 

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    開催年月日: 2014年8月26日 - 2014年8月30日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Stockholm, Sweden  

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  • The role of Casparian strips and Lsi transpoter distribution in efficient Si transport in rice.

    Sakurai, G., Satake, A., Yamaji, N., Mitani, N., Yokozawa, M. and Ma, J. F.

    6th International Conference on Silicon in Agriculture.  2014年 

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    開催年月日: 2014年8月26日 - 2014年8月30日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Stockholm, Sweden  

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  • イネ節によるミネラル分配とその分子機構

    山地直樹

    イネ遺伝学・分子生物学ワークショップ  2014年 

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    開催年月日: 2014年7月11日 - 2014年7月12日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

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  • イネ地上部の鉄分配に関与するクエン酸トランスポーターOsFRDL1.

    横正健剛・山地直樹・馬 建鋒

    第55回日本植物生理学会年会  2014年 

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    開催年月日: 2014年3月18日 - 2014年3月20日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:富山  

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  • イネ節における鉄の分配に関与する輸送体の探索と機能解析.

    山地直樹・佐々木明正・柏野美帆・馬 建鋒

    第55回日本植物生理学会年会  2014年 

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    開催年月日: 2014年3月18日 - 2014年3月20日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:富山  

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  • イネのヒ素耐性と分配に関与する輸送体の解析.

    山木智央・山地直樹・Won Yong Song・Yougsook Lee・馬 建鋒

    第55回日本植物生理学会年会  2014年 

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    開催年月日: 2014年3月18日 - 2014年3月20日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:富山  

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  • イネの節で局在する輸送体OsZIP3は亜鉛の優先的分配に関与する.

    佐々木明正・山地直樹・柏野美帆・三谷奈見季・馬 建鋒

    第55回日本植物生理学会年会  2014年 

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    開催年月日: 2014年3月18日 - 2014年3月20日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:富山  

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  • イネのケイ素輸送におけるカスパリー線の役割とトランスポーター配置.

    櫻井 玄・馬 建鋒・佐竹暁子・横沢正幸・山地直樹・三谷奈見季

    第55回日本植物生理学会年会  2014年 

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    開催年月日: 2014年3月18日 - 2014年3月20日

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:富山  

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  • OsHMA3輸送体の組織局在の改変による低カドミウム米作出の試み.

    馬 建鋒・夏 継星・山地直樹

    第55回日本植物生理学会年会  2014年 

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    開催年月日: 2014年3月18日 - 2014年3月20日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:富山  

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  • イネの亜鉛分配に関与する輸送体遺伝子OsZIP3の機能解析.

    佐々木明正・山地直樹・柏野美帆・馬 建鋒

    第109回日本土壌肥料学会関西支部講演会  2013年 

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    開催年月日: 2013年11月28日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:山口  

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  • イネ野生種におけるケイ酸吸収機構に関する研究.

    三谷奈見季・大貝久生・山地直樹・馬 建鋒

    第109回日本土壌肥料学会関西支部講演会  2013年 

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    開催年月日: 2013年11月28日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:山口  

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  • クエン酸輸送体OsFRDL1はイネの地上部の鉄分配にも関与する.

    横正健剛・山地直樹・馬 建鋒

    第109回日本土壌肥料学会関西支部講演会  2013年 

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    開催年月日: 2013年11月28日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:山口  

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  • イネのマンガン集積に関与する輸送体OsMTP8の解析.

    竹本侑馬・三谷奈見季・佐々木明正・山地直樹・馬 建鋒・加藤伸一郎・岩崎貢三・上野大勢

    第109回日本土壌肥料学会関西支部講演会  2013年 

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    開催年月日: 2013年11月28日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:山口  

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  • Involvement of a magnesium transporter OSMGT1 in alleviating aluminum and salt stress in rice.

    陳 志長・山地直樹・馬 建鋒

    第109回日本土壌肥料学会関西支部講演会  2013年 

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    開催年月日: 2013年11月28日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:山口  

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  • イネのヒ素耐性と集積に関与する輸送体ABCC1.

    山木智央・山地直樹・馬 建鋒

    第109回日本土壌肥料学会関西支部講演会  2013年 

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    開催年月日: 2013年11月28日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:山口  

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  • Promoter analysis of Lsi6 in rice node. Is it possible to reproduce the “Mandara”

    山地直樹

    新学術領域研究「植物の環境突破力」第4回若手の会  2013年 

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    開催年月日: 2013年11月13日 - 2013年11月15日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:宮城  

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  • ミネラルトランスポーターの組織・細胞局在に関する研究.

    山地直樹

    日本土壌肥料学会年会  2013年 

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    開催年月日: 2013年9月11日 - 2013年9月13日

    記述言語:日本語   会議種別:口頭発表(招待・特別)  

    開催地:名古屋  

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  • Translocation of Cu from roots to shoots is mediated by OsHMA5 in rice.

    ? 鋒林・山地直樹・馬 建鋒

    日本土壌肥料学会年会  2013年 

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    開催年月日: 2013年9月11日 - 2013年9月13日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • Characterization of nutrient uptake in a novel short-root mutant of rice.

    夏 継星・山地直樹・馬 建鋒

    日本土壌肥料学会年会  2013年 

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    開催年月日: 2013年9月11日 - 2013年9月13日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • 節を介した亜鉛の分配に関与する輸送体OsZIP3.

    佐々木明正・山地直樹・柏野美帆・馬 建鋒

    日本土壌肥料学会年会  2013年 

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    開催年月日: 2013年9月11日 - 2013年9月13日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • イネ地上部の鉄分配に関与するクエン酸トランスポーターOsFRDL1.

    横正健剛・山地直樹・馬 建鋒

    日本土壌肥料学会年会  2013年 

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    開催年月日: 2013年9月11日 - 2013年9月13日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:名古屋  

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  • 野生イネ由来ケイ酸トランスポーターの解析.

    三谷奈見季・大貝久生・山地直樹・馬 建鋒

    日本土壌肥料学会年会  2013年 

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    開催年月日: 2013年9月11日 - 2013年9月13日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • イネ銅の再転流に関与するトランスポーターの同定.

    鄭 録慶・山地直樹・横正健剛・馬 建鋒

    日本土壌肥料学会年会  2013年 

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    開催年月日: 2013年9月11日 - 2013年9月13日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • Mineral transport from soil to seed.

    Ma, J. F., Yamaji, N., Sasaki, A., Mitani-Ueno, N. and Ueno, D.

    XVII International Plant Nutrition Colloquium 2013  2013年 

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    開催年月日: 2013年8月19日 - 2013年8月22日

    記述言語:英語   会議種別:口頭発表(基調)  

    開催地:Istanbul, Turkey  

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  • Role of node-located transporters in mineral distribution in rice.

    Yamaji, N., Sasaki, A., Xia, J. X., Yokosho, K., Mitani-Ueno, N. and Ma, J. F.

    XVII International Plant Nutrition Colloquium 2013  2013年 

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    開催年月日: 2013年8月19日 - 2013年8月22日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Istanbul, Turkey  

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  • OsNramp5 is a major transporter for uptake of Mn and Cd in rice.

    Sasaki, A., Yamaji, N., Yokosho, K. and Ma, J. F.

    XVII International Plant Nutrition Colloquium 2013  2013年 

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    開催年月日: 2013年8月19日 - 2013年8月22日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Istanbul, Turkey  

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  • Functional characterization of an Al-induced transporter gene in buckwheat.

    Yokosho, K., Yamaji, N. and Ma, J. F.

    XVII International Plant Nutrition Colloquium 2013  2013年 

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    開催年月日: 2013年8月19日 - 2013年8月22日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Istanbul, Turkey  

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  • イネの節におけるミネラルの維管束間輸送と分配制御機構

    山地直樹・佐々木明正・鄭録慶・夏継星・横正健剛・三谷奈見季・馬建鋒

    第8回トランスポーター研究会年会(JTRA2013)  2013年 

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    開催年月日: 2013年6月15日 - 2013年6月16日

    記述言語:日本語   会議種別:口頭発表(招待・特別)  

    開催地:熊本  

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  • Translocation of copper from roots to shoots is mediated by OsHMA5 in rice.

    Deng, F. L., Yonamine, I., Yamaji, N. and Ma, J. F.

    4th Annual Conference on Cost Action FA 0905, Mineral Improved Crop Production for Healthy Food and Feed.  2013年 

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    開催年月日: 2013年6月9日 - 2013年6月13日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Oslo, Norway  

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  • Functional characterization of an Al-induced transporter gene. FeIREG2, in buckwheat.

    Yokosho, K., Yamaji, N. and Ma, J. F.

    16th International Workshop on Plant Membrane Biology.  2013年 

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    開催年月日: 2013年3月26日 - 2013年3月31日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Kurashiki, Japan  

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  • Efficient transport of Si by Lsi1 and Lsi6 is driven by efflux Si transporters in rice.

    Yamaji, N., Mitani-Ueno, N. and Ma, J. F.

    16th International Workshop on Plant Membrane Biology.  2013年 

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    開催年月日: 2013年3月26日 - 2013年3月31日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Kurashiki, Japan  

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  • Transporters conferring mineral stress tolerance.

    Ma, J. F., Yamaji, N., Xia, J. X., Sasaki, A., Huang, C. F. and Chen, Z. C.

    16th International Workshop on Plant Membrane Biology.  2013年 

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    開催年月日: 2013年3月26日 - 2013年3月31日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Kurashiki, Japan  

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  • OsHMA5 is involved in the xylem loading of Cu in rice.

    Deng, F., Yonamine, I., Yamaji, N. and Ma, J. F.

    16th International Workshop on Plant Membrane Biology.  2013年 

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    開催年月日: 2013年3月26日 - 2013年3月31日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • OsYSL16 is required for redistribution of Cu from older tissues in rice.

    Zheng, L. Q., Yamaji, N., Yokosho, K. and Ma, J. F.

    16th International Workshop on Plant Membrane Biology.  2013年 

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    開催年月日: 2013年3月26日 - 2013年3月31日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • Dynamic regulation of Mn distribution by OsNramp3 in rice node.

    Yamaji, N., Sasaki, A., Yokosho, K., Xia, J. X. and Ma, J. F.

    16th International Workshop on Plant Membrane Biology.  2013年 

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    開催年月日: 2013年3月26日 - 2013年3月31日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • OsHKT 1;5-mediated Na+ transport controls a high K/Na ratio of young leaf blades in rice under salinity stress.

    Horie, T., Suzuki, K., Kashiwagi, T., Yamaji, N., An, G. and Ma. J. F.

    16th International Workshop on Plant Membrane Biology.  2013年 

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    開催年月日: 2013年3月26日 - 2013年3月31日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • Identification of a magnesium transporter gene required for aluminum tolerance in rice.

    Chen, Z. C., Yamaji, N. and Ma, J. F.

    16th International Workshop on Plant Membrane Biology.  2013年 

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    開催年月日: 2013年3月26日 - 2013年3月31日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • Functional analysis of OsNramp5, a transporter involved in Mn and Cd uptake in rice.

    Sasaki, A., Ishizaki, K., Yamaji, N., Kohchi, T. and Ma, J. F.

    16th International Workshop on Plant Membrane Biology.  2013年 

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    開催年月日: 2013年3月26日 - 2013年3月31日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • Promoter analysis of a rice influx silicon transporter gene Lsi1.

    Mitani-Ueno, N., Yamaji, N. and Ma, J. F.

    16th International Workshop on Plant Membrane Biology.  2013年 

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    開催年月日: 2013年3月26日 - 2013年3月31日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • A member of Nramp family transports trivalent Al in rice.

    Xia, J. X., Yamaji, N. and Ma, J. F.

    16th International Workshop on Plant Membrane Biology.  2013年 

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    開催年月日: 2013年3月26日 - 2013年3月31日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • イネの銅再転流に関わる輸送体OsYSL16の解析.

    鄭 録慶・山地直樹・横正健剛・馬 建鋒

    第54回日本植物生理学会年会  2013年 

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    開催年月日: 2013年3月21日 - 2013年3月23日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:岡山  

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  • イネ節の維管束間輸送に働くケイ酸輸送体.

    山地直樹・三谷(上野)奈見季・馬 建鋒

    第54回日本植物生理学会年会  2013年 

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    開催年月日: 2013年3月21日 - 2013年3月23日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岡山  

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  • Characterization of loss-of-function mutation in the HKT1;5 gene encoding a Na+ transporter in rice (Oryza sativa).

    Suzuki, K., Kashiwagi, T., Yamaji, N., An, G. H., Ma, J. F., Horie, T.

    第54回日本植物生理学会年会  2013年 

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    開催年月日: 2013年3月21日 - 2013年3月23日

    記述言語:英語   会議種別:ポスター発表  

    開催地:岡山  

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  • ゼニゴケ発現系を用いた植物由来輸送体の輸送活性測定法の確立.

    佐々木明正・石崎公庸・山地直樹・河内孝之・馬 建鋒

    第54回日本植物生理学会年会  2013年 

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    開催年月日: 2013年3月21日 - 2013年3月23日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:岡山  

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  • イネ由来ケイ酸輸送体Lsi1の発現応答およびその制御機構の解析.

    三谷奈見季・山地直樹・馬 建鋒

    第54回日本植物生理学会年会  2013年 

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    開催年月日: 2013年3月21日 - 2013年3月23日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:岡山  

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  • OsHMA5 is a pericycle-localized transporter for Cu in rice.

    Deng, F. L., Yonamine, I., Yamaji, N., Ma, J. F.

    第54回日本植物生理学会年会  2013年 

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    開催年月日: 2013年3月21日 - 2013年3月23日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:岡山  

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  • Up-regulation of a magnesium transporter gene OsMGT1 is required for aluminum tolerance in rice.

    Chen, Z. C., Yamaji, N. and Ma, J. F.

    The 8th International Symposium on Plant-Soil Interactions at Low pH.  2012年 

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    開催年月日: 2012年10月18日 - 2012年10月22日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Bengaluru, India  

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  • Isolation and characterization of an Al-induced transporter gene in buckwheat.

    Yokosho, K., Yamaji, N. and Ma, J. F.

    The 8th International Symposium on Plant-Soil Interactions at Low pH.  2012年 

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    開催年月日: 2012年10月18日 - 2012年10月22日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Bengaluru, India  

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  • OsNramp3-mediated distribution of Mn is controlled by external Mn supply in rice.

    Yamaji, N., Sasaki, A., Yokosho, K., Xia, J. X. and Ma, J. F.

    The 8th International Symposium on Plant-Soil Interactions at Low pH.  2012年 

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    開催年月日: 2012年10月18日 - 2012年10月22日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Bengaluru, India  

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  • Molecular mechanisms of Al tolerance in gramineous crops.

    Ma, J. F., Yamaji, N., Yokosho, K., Xia, J. X., Fujii, M. and Chen, Z.

    The 8th International Symposium on Plant-Soil Interactions at Low pH.  2012年 

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    開催年月日: 2012年10月18日 - 2012年10月22日

    記述言語:英語   会議種別:口頭発表(基調)  

    開催地:Bengaluru, India  

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  • A gene encoding a cysteine-rich peptide is involved in rice Al tolerance.

    Xia, J. X., Yamaji, N., Mitani, N. and Ma, J. F.

    The 8th International Symposium on Plant-Soil Interactions at Low pH.  2012年 

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    開催年月日: 2012年10月18日 - 2012年10月22日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Bengaluru, India  

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  • OsNramp5 is a major transporter responsible for the uptake of manganese in rice.

    Sasaki, A., Yamaji, N., Yokosho, K. and Ma, J. F.

    The 8th International Symposium on Plant-Soil Interactions at Low pH.  2012年 

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    開催年月日: 2012年10月18日 - 2012年10月22日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Bengaluru, India  

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  • OsNramp3によるマンガン分配制御機構.

    佐々木明正・山地直樹・馬 建鋒

    日本土壌肥料学会 

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    開催年月日: 2012年9月4日 - 2012年9月6日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:鳥取大学  

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  • ケイ酸輸送体Lsi1のケイ酸応答性および組織局在性を制御するプロモーターの解析.

    三谷奈見季・山地直樹・馬 建鋒

    日本土壌肥料学会 

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    開催年月日: 2012年9月4日 - 2012年9月6日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:鳥取大学  

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  • イネのOsHMA2による亜鉛およびカドミウムの選択的分配機構.

    山地直樹・夏 継星・馬 建鋒

    日本土壌肥料学会 

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    開催年月日: 2012年9月4日 - 2012年9月6日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:鳥取大学  

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  • オオムギのアルミニウム耐性機構の獲得.

    柏野美帆・横正健剛・山地直樹・最相大輔・山根美樹・高橋宏和・佐藤和広・中園幹生・馬 建鋒

    日本土壌肥料学会 

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    開催年月日: 2012年9月4日 - 2012年9月6日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:鳥取大学  

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  • イネケイ酸輸送体Lsi2(SIET1)のホモログSIET3-5の解析.

    久家徳之・山地直樹・馬 建鋒

    日本土壌肥料学会 

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    開催年月日: 2012年9月4日 - 2012年9月6日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:鳥取大学  

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  • イネのMATE遺伝子OsFRDL2の更なる機能解析.

    横正健剛・山地直樹・馬 建鋒

    日本土壌肥料学会 

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    開催年月日: 2012年9月4日 - 2012年9月6日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:鳥取大学  

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  • Knockout of a Mg transporter gene resulted in increased Al sensitivity in rice.

    Chen, Z. C., Yamaji, N. and Ma, J. F.

    International Symposium on Magnesium in Crop Production, Food Quality and Human Health. 

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    開催年月日: 2012年5月8日 - 2012年5月9日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Goettingen, Germany  

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  • OsNramp3によるイネの選択的マンガン分配機構

    山地直樹・佐々木明正・夏 継星・馬 建鋒

    日本植物生理学会 

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    開催年月日: 2012年3月16日 - 2012年3月18日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都産業大学  

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  • OsMTP9 is required for root-to-shoot Mn translocation in rice.

    Fujii, Y., Akagi, Y., Chen, Z., Iwasaki, K., Kato, S., Yamaji, N., Ma, J. F. and Ueno, D.

    日本植物生理学会 

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    開催年月日: 2012年3月16日 - 2012年3月18日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都産業大学  

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  • イネのマンガン吸収に関与する輸送体遺伝子の機能解析

    佐々木明正・山地直樹・馬 建鋒

    日本植物生理学会 

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    開催年月日: 2012年3月16日 - 2012年3月18日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都産業大学  

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  • ソバのアルミニウム誘導性輸送体遺伝子(FeIREG2)の機能解析.

    横正健剛・山地直樹・馬 建鋒

    日本植物生理学会 

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    開催年月日: 2012年3月16日 - 2012年3月18日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:京都産業大学  

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  • OsMTP8.1 contribute to Mn tolerance by sequestering Mn to vacuoles in shoot of rice.

    Ueno, D., Chen, Z., Kamiya, T., Iwasaki, K., Kato, S., Yamaji, N. and Ma, J. F.

    日本植物生理学会 

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    開催年月日: 2012年3月16日 - 2012年3月18日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:京都産業大学  

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  • A putative ER-localized magnesium transporter OsMGT1 is involved in aluminum tolerance in rice.

    Chen, Z.C., Yamaji, N. and Ma, J. F

    日本植物生理学会 

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    開催年月日: 2012年3月16日 - 2012年3月18日

    記述言語:英語   会議種別:ポスター発表  

    開催地:京都産業大学  

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  • An ER-localized magnesium transporter OsMGT1 is required for aluminum tolerance in rice.

    Chen, Z. C., Yamaji, N. and Ma, J. F.

    International Symposium “Strategies of Plants against Global Environmental Change” 

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    開催年月日: 2011年12月8日 - 2011年12月10日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • Characterization of FeIREG2, which is an Al-induced transporter gene in buckwheat.

    Yokosho, K., Yamaji, N. and Ma, J. F.

    International Symposium “Strategies of Plants against Global Environmental Change” 

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    開催年月日: 2011年12月8日 - 2011年12月10日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • OsYSL16 is a phloem-localized transporter for Cu distribution in rice.

    Zheng, L. Q., Yamaji, N. and Ma, J. F.

    International Symposium “Strategies of Plants against Global Environmental Change” 

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    開催年月日: 2011年12月8日 - 2011年12月10日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • OsCDT3 encoding a cysteine rich peptide is involved in rice Al tolerance.

    Xia, J. X., Yamaji, N., Mitani-Ueno, N. and Ma, J. F.

    International Symposium “Strategies of Plants against Global Environmental Change” 

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    開催年月日: 2011年12月8日 - 2011年12月10日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • Functional analysis of OsNramp5, a gene involved in manganese uptake in rice.

    Sasaki, A., Yamaji, N. and Ma, J. F.

    International Symposium “Strategies of Plants against Global Environmental Change” 

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    開催年月日: 2011年12月8日 - 2011年12月10日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • Functional analysis of a transcription factor ART2 for tolerance to Al rhizotoxicity in rice.

    Tsutsui, T., Yamaji, N. and Ma, J. F.

    International Symposium “Strategies of Plants against Global Environmental Change” 

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    開催年月日: 2011年12月8日 - 2011年12月10日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • Mechanism regulating HvAACT1 expression in barley.

    Fujii, M., Yokosho, K., Yamaji, N. and Ma, J. F.

    International Symposium “Strategies of Plants against Global Environmental Change” 

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    開催年月日: 2011年12月8日 - 2011年12月10日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • Novel findings of efficient mineral distribution mechanisms in rice node.

    Yamaji, N. and Ma, J. F.

    International Symposium “Strategies of Plants against Global Environmental Change” 

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    開催年月日: 2011年12月8日 - 2011年12月10日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Kurashiki, Japan  

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  • ART1-regulated genes involved in tolerance to Al rhizotoxicity.

    Yamaji, N. and Ma, J. F.

    Rhizosphere 3 International Conference. 

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    開催年月日: 2011年9月25日 - 2011年9月30日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Perth, Australia  

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  • Isolation and characterization of an Al-induced transporter gene in buckwheat.

    Yokosho, K., Yamaji, N. and Ma, J. F.

    Rhizosphere 3 International Conference. 

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    開催年月日: 2011年9月25日 - 2011年9月30日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Perth, Australia  

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  • Mechanism regulating HvAACT1 expression in barley.

    Fujii, M., Yamaji, N. and Ma, J. F.

    Rhizosphere 3 International Conference. 

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    開催年月日: 2011年9月25日 - 2011年9月30日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Perth, Australia  

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  • Functional analysis of a transcription factor ART2 for tolerance to Al rhizotoxicity in rice.

    Tsutsui, T., Yamaji, N. and Ma, J. F.

    Rhizosphere 3 International Conference. 

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    開催年月日: 2011年9月25日 - 2011年9月30日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Perth, Australia  

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  • Characterization of Si uptake and identification of Si transporter genes in wild rice.

    Oogai, H., Yamaji, N. and Ma, J. F.

    5th International Conference on Silicon in Agriculture. 

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    開催年月日: 2011年9月13日 - 2011年9月18日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Beijing, China  

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  • Transporters involved in preferential distribution of Si to the panicles at the node in rice.

    Yamaji, N., Mitani-Ueno, N. and Ma, J. F.

    5th International Conference on Silicon in Agriculture. 

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    開催年月日: 2011年9月13日 - 2011年9月18日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Beijing, China  

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  • Transport of silicon from roots to panicles in different plant species.

    Ma, J. F., Yamaji, N. and Mitani-Ueno, N.

    5th International Conference on Silicon in Agriculture. 

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    開催年月日: 2011年9月13日 - 2011年9月18日

    記述言語:英語   会議種別:口頭発表(基調)  

    開催地:Beijing, China  

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  • Isolation and functional analysis of Si transporters in two pumpkin cultivars contrasting in Si uptake.

    Mitani-Ueno, N., Yamaji, N., Ago, Y., Iwasaki, K. and Ma, J. F.

    5th International Conference on Silicon in Agriculture. 

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    開催年月日: 2011年9月13日 - 2011年9月18日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Beijing, China  

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  • ソバのアルミニウム誘導性トランスポーターの単離と機能解析.

    横正健剛・山地直樹・馬 建鋒

    日本土壌肥料学会年会  2011年 

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    開催年月日: 2011年8月8日 - 2011年8月10日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:つくば  

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  • ケイ酸輸送体Lsi1の発現制御機構の解析.

    三谷奈見季・山地直樹・馬 建鋒

    日本土壌肥料学会年会  2011年 

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    開催年月日: 2011年8月8日 - 2011年8月10日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:つくば  

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  • イネのマンガン分配に関与するOsNramp3の機能解析.

    山地直樹・佐々木明正・夏 継星・馬 建鋒

    日本土壌肥料学会年会  2011年 

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    開催年月日: 2011年8月8日 - 2011年8月10日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:つくば  

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  • イネのマンガン吸収に関与する輸送体遺伝子の機能解析.

    佐々木明正・山地直樹・馬 建鋒

    日本土壌肥料学会年会  2011年 

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    開催年月日: 2011年8月8日 - 2011年8月10日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:つくば  

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  • イネの高マンガン集積に関与するトランスポーター遺伝子の単離と解析.

    上野大勢・陳 宗慧・岩崎貢三・加藤伸一郎・馬 建鋒

    日本土壌肥料学会年会  2011年 

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    開催年月日: 2011年8月8日 - 2011年8月10日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:つくば  

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  • イネ亜ヒ酸排出能の品種間差.

    大貝久生・山地直樹・三谷奈見季・馬 建鋒

    日本土壌肥料学会年会  2011年 

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    開催年月日: 2011年8月8日 - 2011年8月10日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:つくば  

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  • Identification of the transporter involved in Mn tolerance in rice.

    陳 宗慧・神谷岳洋・岩崎貢三・加藤伸一郎・山地直樹・馬 建鋒・上野大勢

    日本土壌肥料学会年会  2011年 

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    開催年月日: 2011年8月8日 - 2011年8月10日

    記述言語:英語   会議種別:ポスター発表  

    開催地:つくば  

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  • The biology of silicon.

    Ma, J. F., Yamaji, N. and Mitani-Ueno, N.

    The 4th Workshop on the Aqueous Chemistry and Biochemistry of Silicon.  2011年 

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    開催年月日: 2011年6月20日 - 2011年6月23日

    記述言語:英語   会議種別:口頭発表(基調)  

    開催地:London, UK  

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  • イネケイ酸輸送体OsNIP2;1の基質選択性の解析.

    三谷奈見季・山地直樹・Zhao, F-J.・馬 建鋒

    第3回植物アクアポリン研究検討会  2011年 

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    開催年月日: 2011年6月3日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:倉敷  

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  • ケイ酸トランスポーターLsi1は亜ヒ酸も亜セレン酸も輸送する.

    馬 建鋒・山地直樹・三谷奈見季

    第3回植物アクアポリン研究検討会  2011年 

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    開催年月日: 2011年6月3日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:倉敷  

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  • Isolation and functional characterization of silicon transporters in two pumpkin cultivars contrasting in silicon accumulation.

    Mitani, N., Yamaji, N., Ago, Y., Iwasaki, K. and Ma, J. F.

    3rd Japan-China Joint Workshop on Plant Nutrition.  2011年 

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    開催年月日: 2011年3月27日 - 2011年3月29日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Kurashiki, Japan  

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  • Citrate-transporting MATE proteins play two different physiological roles in plants.

    Yokosho, K., Yamaji, N. and Ma, J. F.

    3rd Japan-China Joint Workshop on Plant Nutrition.  2011年 

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    開催年月日: 2011年3月27日 - 2011年3月29日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Kurashiki, Japan  

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  • Preferential Si distribution to the panicles mediated by transporters for inter-vascular transfer at rice node.

    Yamaji, N., Mitani, N. and Ma, J. F.

    3rd Japan-China Joint Workshop on Plant Nutrition.  2011年 

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    開催年月日: 2011年3月27日 - 2011年3月29日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Kurashiki, Japan  

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  • OsHMA3, a transporter limiting Cd accumulation in rice.

    Ueno, D., Yamaji, N., Kono, I., Huang, C. F., Ando, T., Yano, M. and Ma, J. F.

    3rd Japan-China Joint Workshop on Plant Nutrition.  2011年 

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    開催年月日: 2011年3月27日 - 2011年3月29日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Kurashiki, Japan  

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  • イネの節で高発現するOsNramp3の機能解析.

    山地直樹・夏 継星・佐々木明正・馬 建鋒

    第52回日本植物生理学会年会  2011年 

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    開催年月日: 2011年3月20日 - 2011年3月22日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台  

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  • OsYSL16 is a transporter of Cu-nicotianamine complex for Cu translocation in rice.

    Zheng, L.Q., Yamaji, N., Ma, J. F.

    第52回日本植物生理学会年会  2011年 

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    開催年月日: 2011年3月20日 - 2011年3月22日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:仙台  

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  • イネのカドミウム集積を抑制する遺伝子.

    馬 建鋒・上野大勢・山地直樹・矢野昌裕

    第52回日本植物生理学会年会  2011年 

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    開催年月日: 2011年3月20日 - 2011年3月22日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台  

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  • A gene encoding a cysteine-rich peptide is involved in rice Al tolerance.

    Xia, J. X., Yamaji, N., Ma, J. F.

    第52回日本植物生理学会年会  2011年 

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    開催年月日: 2011年3月20日 - 2011年3月22日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:仙台  

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  • イネアルミニウム応答性転写調節因子ART1のシス配列の同定.

    筒井友和・山地直樹・馬 建鋒

    第52回日本植物生理学会年会  2011年 

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    開催年月日: 2011年3月20日 - 2011年3月22日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台  

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  • ソバのアルミニウム誘導性輸送体遺伝子の単離と機能解析.

    横正健剛・山地直樹・馬 建鋒

    第52回日本植物生理学会年会  2011年 

     詳細を見る

    開催年月日: 2011年3月20日 - 2011年3月22日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:仙台  

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  • OsYSL6 is involved in detoxification of excess manganese in rice.

    Sasaki, A., Yamaji, N. and Ma, J. F.

    Genetics of Plant Mineral Nutrition  2010年 

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    開催年月日: 2010年9月30日 - 2010年10月2日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Hannover, Germany  

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  • Transporters involved in the accumulation and hypertolerance of Cd in plants.

    Ma, J. F., Ueno, D. and Yamaji, N.

    Plant Membrane Biology, 15th International Workshop Adelaide 2010  2010年 

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    開催年月日: 2010年9月19日 - 2010年9月24日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Adelaide, Australia  

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  • Functional analysis of three MATE genes encoding citrate transporter in rice.

    Yokosho, K., Yamaji, N., Mitani, N. and Ma, J. F.

    Plant Membrane Biology, 15th International Workshop Adelaide 2010  2010年 

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    開催年月日: 2010年9月19日 - 2010年9月24日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Adelaide, Australia  

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  • Transporters involved in inter-vascular transfer of Si at the node of rice.

    Yamaji, N., Mitani, N. and Ma, J. F.

    Plant Membrane Biology, 15th International Workshop Adelaide 2010  2010年 

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    開催年月日: 2010年9月19日 - 2010年9月24日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Adelaide, Australia  

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  • Role of OsALS1 in Al tolerance in rice.

    Huang, C. F., Yamaji, N. and Ma, J. F.

    International Symposium on Biodiversity Sciences.  2010年 

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    開催年月日: 2010年7月31日 - 2010年8月3日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Nagoya, Japan  

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  • Expression profiling of metal transporter genes responsible for the preferential distribution in rice node.

    Yamaji, N. and Ma, J. F.

    15th International Symposium on Iron Nutrition and Interactions in Plants.  2010年 

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    開催年月日: 2010年6月26日 - 2010年6月30日

    記述言語:英語   会議種別:ポスター発表  

    開催地:Budapest, Hungary  

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  • オオムギとイネのケイ酸吸収機構の違いに関する研究

    三谷奈見季・山地直樹・馬 建鋒

    第51回日本植物生理学会年会  2010年 

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    開催年月日: 2010年3月18日 - 2010年3月21日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:熊本  

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  • オオムギのケイ素分配に関与するトランスポーターの解析

    馬 建鋒・山地直樹・千葉由佳子・三谷奈見季

    第51回日本植物生理学会年会  2010年 

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    開催年月日: 2010年3月18日 - 2010年3月21日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:熊本  

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  • ライムギ由来MATE遺伝子(ScFRDL1とScFRDL2)の単離と機能解析

    横正健剛・山地直樹・馬 建鋒

    第51回日本植物生理学会年会  2010年 

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    開催年月日: 2010年3月18日 - 2010年3月21日

    記述言語:日本語   会議種別:ポスター発表  

    開催地:熊本  

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  • イネのカドミウム集積に関与する輸送体CASTLE1の単離と解析.

    上野大勢・山地直樹・河野いづみ・黄 朝鋒・安藤露・矢野昌裕・馬 建鋒

    第51回日本植物生理学会年会  2010年 

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    開催年月日: 2010年3月18日 - 2010年3月21日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:熊本  

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  • イネの新規ケイ酸輸送体Lsi3の解析

    山地直樹・三谷奈見季・馬 建鋒

    第51回日本植物生理学会年会  2010年 

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    開催年月日: 2010年3月18日 - 2010年3月21日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:熊本  

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  • イネアルミニウムトランスポーターの同定

    夏 継星・山地直樹・馬 建鋒

    第51回日本植物生理学会年会  2010年 

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    開催年月日: 2010年3月18日 - 2010年3月21日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:熊本  

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  • A bacterial-type ABC transporter for detoxification of aluminum in plants.

    Ma, J. F., Huang, C. F. and Yamaji, N.

    3rd FEBS Special Meeting on ABC Proteins.  2010年 

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    開催年月日: 2010年2月27日 - 2010年3月5日

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    開催地:Innsbruck, Austria  

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  • Plant silicon transporters.

    Ma, J.F. and Yamaji, N.

    3rd Workshop on the Aqueous Chemistry and Biochemistry of Silicon. 

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    開催年月日: 2009年12月9日 - 2009年12月11日

    記述言語:英語   会議種別:口頭発表(基調)  

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  • Silicon distribution by a rice aquaporin, Lsi6.

    Yamaji, N., Mitani, N. and Ma, J.F.

    3rd Workshop on the Aqueous Chemistry and Biochemistry of Silicon. 

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    開催年月日: 2009年12月9日 - 2009年12月11日

    記述言語:英語   会議種別:ポスター発表  

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  • An efflux transporter of silicon in barley roots.

    Mitani, N., Chiba, Y., Yamaji, N. and Ma, J.F.

    3rd Workshop on the Aqueous Chemistry and Biochemistry of Silicon. 

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    開催年月日: 2009年12月9日 - 2009年12月11日

    記述言語:英語   会議種別:ポスター発表  

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  • 節のケイ酸分配機構

    山地直樹、馬 建鋒

    特定領域研究「植物膜輸送」ワークショップ 

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    開催年月日: 2009年10月1日 - 2009年10月3日

    記述言語:日本語   会議種別:口頭発表(一般)  

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  • イネの鉄輸送とアルミニウム無毒化におけるOsFRDL2の役割.

    横正健剛・*山地直樹・馬 建鋒

    日本土壌肥料学会年会 

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    開催年月日: 2009年9月15日 - 2009年9月18日

    会議種別:口頭発表(一般)  

    開催地:京都  

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  • ブルームレスキュウリ用カボチャ台木のケイ酸吸収特性のさらなる解析.

    馬 建鋒・吾郷幸子・*山地直樹・三谷奈見季・岩崎貢三

    日本土壌肥料学会年会 

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    開催年月日: 2009年9月15日 - 2009年9月18日

    会議種別:口頭発表(一般)  

    開催地:京都  

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  • Cd超集積植物Thlaspi caerulescens (ecotype, Ganges)由来のP-type ATPase HMA3の機能.

    上野大勢・*山地直樹・馬 建鋒

    日本土壌肥料学会年会 

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    開催年月日: 2009年9月15日 - 2009年9月18日

    会議種別:口頭発表(一般)  

    開催地:京都  

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  • イネのケイ素分配の分子機構(I).

    *山地直樹・馬 建鋒

    日本土壌肥料学会年会 

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    開催年月日: 2009年9月15日 - 2009年9月18日

    会議種別:口頭発表(一般)  

    開催地:京都  

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  • A rice Nramp gene is involved in Al tolerance.

    Jixing Xia・*山地直樹・馬 建鋒

    日本土壌肥料学会年会 

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    開催年月日: 2009年9月15日 - 2009年9月18日

    会議種別:口頭発表(一般)  

    開催地:京都  

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  • Cloning and functional analysis of a rice gene involved in the tolerance to multiple metal toxicity.

    黄 朝鋒・*山地直樹・田島茂行・矢野昌裕・馬 建鋒

    日本土壌肥料学会年会 

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    開催年月日: 2009年9月15日 - 2009年9月18日

    会議種別:口頭発表(一般)  

    開催地:京都  

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  • Maize silicon transporters.

    Mitani, N., *Yamaji, N. and Ma, J.F.

    XVI International Plant Nutrition Colloquium 2009. 

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    開催年月日: 2009年8月26日 - 2009年8月30日

    会議種別:ポスター発表  

    開催地:California, USA.  

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  • Mechanism regulating HvAACT1 expression in barley.

    Fujii, M., *Yamaji, N., Sato, K. and Ma, J.F.

    Plant Biology 2009. 

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    開催年月日: 2009年6月18日 - 2009年6月22日

    会議種別:ポスター発表  

    開催地:Honolulu, Hawaii. USA  

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  • Functional analysis of MATE family, citrate transporter genes in rice.

    Yokosho, K., Ueno, D., *Yamaji, N., Mitani, N. and Ma, J.F.

    Plant Biology 2009. 

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    開催年月日: 2009年6月18日 - 2009年6月22日

    会議種別:ポスター発表  

    開催地:Honolulu, Hawaii. USA  

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  • Transporters involved in uptake and distribution of silicon in rice.

    Ma, J.F., *Yamaji, N. and Mitani, N.

    Plant Biology 2009. 

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    開催年月日: 2009年6月18日 - 2009年6月22日

    会議種別:ポスター発表  

    開催地:Honolulu, Hawaii. USA  

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  • Mechanism regulating HvAACT1 expression in barley.

    Fujii, M., *Yamaji, N., Sato, K. and Ma, J.F.

    7th International Symposium on Plant-Soil Interactions at Low pH. 

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    開催年月日: 2009年5月17日 - 2009年5月21日

    会議種別:ポスター発表  

    開催地:Guangzhou, China.  

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  • Transporters involved in Al resistance in gramineous crops.

    Ma, J.F., Huang, C.F., Furukawa, J. and *Yamaji, N.

    7th International Symposium on Plant-Soil Interactions at Low pH. 

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    開催年月日: 2009年5月17日 - 2009年5月21日

    会議種別:口頭発表(一般)  

    開催地:Guangzhou, China.  

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  • Genome-wide transcriptional analysis of Al-responsive genes in rice.

    Tsutsui, T., Huang, C.F., *Yamaji, N., Nagamura, Y. and Ma, J.F.

    7th International Symposium on Plant-Soil Interactions at Low pH. 

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    開催年月日: 2009年5月17日 - 2009年5月21日

    会議種別:ポスター発表  

    開催地:Guangzhou, China.  

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  • Isolation and characterization of an Al-tolerance gene STAR1 in rice.

    Huang, C.F., *Yamaji, N., Tajima, S., Yano, M. and Ma, J.F.

    7th International Symposium on Plant-Soil Interactions at Low pH. 

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    開催年月日: 2009年5月17日 - 2009年5月21日

    会議種別:口頭発表(一般)  

    開催地:Guangzhou, China.  

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  • Identification of a transcription factor regulating high Al resistance in rice.

    *Yamaji, N., Huang, C.F., Nagamura, Y. and Ma, J.F.

    7th International Symposium on Plant-Soil Interactions at Low pH. 

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    開催年月日: 2009年5月17日 - 2009年5月21日

    会議種別:口頭発表(一般)  

    開催地:Guangzhou, China.  

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  • Functional analysis of OsFRDL4, a citrate transporter gene induced by aluminum.

    Yokosho, K., *Yamaji, N. and Ma, J.F.

    7th International Symposium on Plant-Soil Interactions at Low pH. 

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    開催年月日: 2009年5月17日 - 2009年5月21日

    会議種別:口頭発表(一般)  

    開催地:Guangzhou, China.  

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  • オオムギアルミニウム活性型クエン酸トランスポーター遺伝子(HvAACT1)の発現調節機構に関する研究.

    藤井美帆・*山地直樹・佐藤和広・馬 建鋒

    第50回日本植物生理学会年会 

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    開催年月日: 2009年3月21日 - 2009年3月24日

    会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • カドミウム超集積植物Thlaspi caerulescens (ecotype, Ganges)由来のP-type ATPase HMA3の単離と機能解析.

    上野大勢・*山地直樹・馬 建鋒

    第50回日本植物生理学会年会 

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    開催年月日: 2009年3月21日 - 2009年3月24日

    会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • マイクロアレイによるイネアルミニウム応答遺伝子の網羅的解析.

    筒井友和・黄 朝鋒・*山地直樹・長村吉晃・馬 建鋒

    第50回日本植物生理学会年会 

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    開催年月日: 2009年3月21日 - 2009年3月24日

    会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • イネアルミニウム耐性におけるOsFRDL4の機能解析.

    横正健剛・*山地直樹・馬 建鋒

    第50回日本植物生理学会年会 

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    開催年月日: 2009年3月21日 - 2009年3月24日

    会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • ケイ酸輸送体Lsi6によるイネ生殖生長期のケイ酸分配.

    *山地直樹・馬 建鋒

    第50回日本植物生理学会年会 

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    開催年月日: 2009年3月21日 - 2009年3月24日

    会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • オオムギ由来排出型ケイ酸輸送体HvLsi2の更なる機能解析.

    三谷奈見季・*山地直樹・千葉由佳子・馬 建鋒

    第50回日本植物生理学会年会 

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    開催年月日: 2009年3月21日 - 2009年3月24日

    会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • カボチャ及びトマトのケイ酸吸収特性と関連遺伝子の単離

    (1) 馬 建鋒・吾郷幸子・*山地直樹・三谷奈見季・岩崎貢三

    日本土壌肥料学会関西支部会 

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    開催年月日: 2008年11月28日

    会議種別:口頭発表(一般)  

    開催地:徳島  

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  • Silicon transporters in rice.

    *Yamaji, N., Mitani, N. and Ma, J.F.

    Silicon in Agriculture Conference South Africa 2008. 

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    開催年月日: 2008年10月26日 - 2008年10月31日

    会議種別:口頭発表(一般)  

    開催地:KwaZulu-Natal, South Africa.  

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  • Silicon transporters in maize and barley-comparison of silicon transporters in different plant species.

    Mitani, N., Chiba, Y., *Yamaji, N. and Ma, J.F.

    Silicon in Agriculture Conference South Africa 2008. 

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    開催年月日: 2008年10月26日 - 2008年10月31日

    会議種別:ポスター発表  

    開催地:KwaZulu-Natal, South Africa.  

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  • Characterization of silicon transporters from rice and maize.

    (8) Mitani, N., *Yamaji, N. and Ma, J.F.

    Silicon in Agriculture Conference South Africa 2008. 

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    開催年月日: 2008年10月26日 - 2008年10月31日

    会議種別:口頭発表(一般)  

    開催地:KwaZulu-Natal, South Africa.  

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  • Differential uptake of silicon in two cultivars of pumpkin.

    (4) Ago, Y., Mitani, N., *Yamaji, N., Iwasaki, K. and Ma, J. F.

    Silicon in Agriculture Conference South Africa 2008 

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    開催年月日: 2008年10月26日 - 2008年10月31日

    会議種別:ポスター発表  

    開催地:KwaZulu-Natal, South Africa.  

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  • Functional analysis of OsFRDL1, a citrate transporter involved in Fe translocation in rice. XIV International Symposium on Iron Nutrition and Interactions in Plants.

    (2) Yokosyo, K., Ueno, D., *Yamaji, N., Mitani, N. and Ma, J. F.

    XIV International Symposium on Iron Nutrition and Interactions in Plants. 

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    開催年月日: 2008年10月11日 - 2008年10月16日

    会議種別:ポスター発表  

    開催地:Beijing, China.  

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  • イネ転写調節因子STAR3によるアルミニウム耐性遺伝子の発現制御

    *山地直樹、黄朝鋒、長村吉晃、馬建鋒

    日本土壌肥料学会 

     詳細を見る

    開催年月日: 2008年9月9日 - 2008年9月11日

    会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • ケイ酸輸送体と吸収・集積機構

    山地直樹

    日本土壌肥料学会 

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    開催年月日: 2008年9月9日 - 2008年9月11日

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(指名)  

    開催地:名古屋  

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  • 異なるイネ科植物由来のケイ酸トランスポーター特性の比較解析

    三谷奈見季、千葉由佳子、*山地直樹、馬建鋒

    日本土壌肥料学会 

     詳細を見る

    開催年月日: 2008年9月9日 - 2008年9月11日

    会議種別:ポスター発表  

    開催地:名古屋  

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  • 植物の金属ストレスにおけるMATE遺伝子の機能解析

    横正健剛、上野大勢、*山地直樹、三谷奈見季、馬建鋒

    日本土壌肥料学会 

     詳細を見る

    開催年月日: 2008年9月9日 - 2008年9月11日

    会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • イネのヒ素吸収に関与するトランスポーターの同定

    馬建鋒、*山地直樹、三谷奈見季、Fangjie Zhao

    日本土壌肥料学会 

     詳細を見る

    開催年月日: 2008年9月9日 - 2008年9月11日

    会議種別:口頭発表(一般)  

    開催地:名古屋  

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  • 新規アルミニウム感受性突然変異体の単離と原因遺伝子のマッピング

    黄朝鋒、*山地直樹、田島茂行、馬建鋒

    日本土壌肥料学会 

     詳細を見る

    開催年月日: 2008年9月9日 - 2008年9月11日

    会議種別:ポスター発表  

    開催地:名古屋  

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  • カボチャ2品種のケイ酸吸収特性の解析

    吾郷幸子、三谷奈見季、*山地直樹、岩崎貢三、馬建鋒

    日本土壌肥料学会 

     詳細を見る

    開催年月日: 2008年9月9日 - 2008年9月11日

    会議種別:ポスター発表  

    開催地:名古屋  

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  • イネのアルミニウム耐性に関与する細菌タイプABCトランスポーターの解析

    馬建鋒、*山地直樹、黄朝鋒、三谷奈見季

    日本植物生理学会 

     詳細を見る

    開催年月日: 2008年3月20日 - 2008年3月22日

    会議種別:口頭発表(一般)  

    開催地:札幌  

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  • イネの鉄栄養に関与するクエン酸トランスポーターOsFRDL1の機能解析

    横正健剛、上野大勢、*山地直樹、三谷奈見季、馬建鋒

    日本植物生理学会 

     詳細を見る

    開催年月日: 2008年3月20日 - 2008年3月22日

    会議種別:口頭発表(一般)  

    開催地:札幌  

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  • Isolation and characterization of a novel rice mutant sensitive to Al and other metals.

    Chaofeng Huang, *Naoki Yamaji, Jian Feng Ma

    日本植物生理学会 

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    開催年月日: 2008年3月20日 - 2008年3月22日

    会議種別:ポスター発表  

    開催地:札幌  

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  • オオムギにおけるケイ酸トランスポーターの解析

    千葉由佳子、三谷奈見季、*山地直樹、馬建鋒

    日本植物生理学会 

     詳細を見る

    開催年月日: 2008年3月20日 - 2008年3月22日

    会議種別:口頭発表(一般)  

    開催地:札幌  

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  • トウモロコシのケイ酸トランスポーターの同定

    三谷奈見季、*山地直樹、馬建鋒

    日本植物生理学会 

     詳細を見る

    開催年月日: 2008年3月20日 - 2008年3月22日

    会議種別:口頭発表(一般)  

    開催地:札幌  

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  • イネのヒ素吸収におけるケイ酸輸送体の関与

    *山地直樹、三谷奈見季、Xiao-Yan Xu、Steve P. McGrath、Fang-Jie Zhao、馬建鋒

    日本植物生理学会 

     詳細を見る

    開催年月日: 2008年3月20日 - 2008年3月22日

    会議種別:口頭発表(一般)  

    開催地:札幌  

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  • オオムギにおけるアルミニウムによるクエン酸分泌に関与する遺伝子の単離と機能解析

    古川純、*山地直樹、王華、三谷奈見季、村田佳子、佐藤和広、且原真木、武田和広、馬建鋒

    日本土壌肥料学会 

     詳細を見る

    開催年月日: 2007年8月22日 - 2007年8月24日

    会議種別:口頭発表(一般)  

    開催地:東京  

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  • トウモロコシ由来のケイ酸吸収遺伝子の単離と解析

    三谷奈見季、*山地直樹、馬建鋒

    日本土壌肥料学会 

     詳細を見る

    開催年月日: 2007年8月22日 - 2007年8月24日

    会議種別:口頭発表(一般)  

    開催地:東京  

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  • Functional analyses of two Al-tolerant genes Als1 and Als2 in rice.

    黄朝鋒、*山地直樹、田島茂行、矢野昌裕、馬建鋒

    日本土壌肥料学会 

     詳細を見る

    開催年月日: 2007年8月22日 - 2007年8月24日

    会議種別:口頭発表(一般)  

    開催地:東京  

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  • 大麦におけるケイ酸吸収機構の解析

    千葉由佳子、三谷奈見季、*山地直樹、馬建鋒

    日本土壌肥料学会 

     詳細を見る

    開催年月日: 2007年8月22日 - 2007年8月24日

    会議種別:ポスター発表  

    開催地:東京  

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  • イネのケイ酸輸送体と吸収・集積機構

    *山地直樹、三谷奈見季、玉井一規、馬建鋒

    日本土壌肥料学会 

     詳細を見る

    開催年月日: 2007年8月22日 - 2007年8月24日

    会議種別:ポスター発表  

    開催地:東京  

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  • Functional analysis of silicon efflux transporter Lsi2 in rice

    *Naoki Yamaji, Namiki Mitani,Kazunori Tamai, Jian Feng Ma

    XIV International Workshop Plant Membrane Biology 

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    開催年月日: 2007年6月26日 - 2007年6月30日

    会議種別:ポスター発表  

    開催地:Valencia, Spain  

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  • Silicon transporters in rice

    Jian Feng Ma, *Naoki Yamaji, Namiki Mitani

    XIV International Workshop Plant Membrane Biology 

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    開催年月日: 2007年6月26日 - 2007年6月30日

    会議種別:口頭発表(一般)  

    開催地:Valencia, Spain  

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  • イネ科NIPのケイ酸輸送特性の解析

    三谷奈見季、*山地直樹、且原真木、馬建鋒

    日本植物生理学会 

     詳細を見る

    開催年月日: 2007年3月28日 - 2007年3月30日

    会議種別:口頭発表(一般)  

    開催地:松山  

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  • トウモロコシ由来の鉄ームギネ酸錯体輸送体遺伝子ZmYS1の更なる解析

    上野大勢、*山地直樹、馬建鋒

    日本植物生理学会 

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    開催年月日: 2007年3月28日 - 2007年3月30日

    会議種別:口頭発表(一般)  

    開催地:松山  

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  • イネケイ酸輸送体Lsi6の機能解析

    *山地直樹、三谷奈見季、馬建鋒

    日本植物生理学会 

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    開催年月日: 2007年3月28日 - 2007年3月30日

    会議種別:口頭発表(一般)  

    開催地:松山  

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  • オオムギのアルミニウム活性型クエン酸輸送体候補遺伝子の発現ならびに機能解析

    古川純、*山地直樹、王華、且原真木、佐藤和広、武田和義、馬建鋒

    日本植物生理学会 

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    開催年月日: 2007年3月28日 - 2007年3月30日

    会議種別:口頭発表(一般)  

    開催地:松山  

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  • イネアルミニウム耐性遺伝子Als1の機能解析

    黄朝鋒、*山地直樹、矢野昌裕、馬建鋒

    日本植物生理学会 

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    開催年月日: 2007年3月28日 - 2007年3月30日

    会議種別:口頭発表(一般)  

    開催地:松山  

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  • イネケイ酸吸収関連遺伝子Lsi2の解析

    玉井一規、*山地直樹、三谷奈見季、小西佐江子、矢野昌裕、馬建鋒

    日本土壌肥料学会 

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    開催年月日: 2006年9月5日 - 2006年9月7日

    開催地:秋田  

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  • オオムギの鉄?ムギネ酸錯体トランスポーター遺伝子の単離と解析

    馬 建鋒、上野 大勢、*山地直樹、村田佳子

    日本土壌肥料学会 

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    開催年月日: 2006年9月5日 - 2006年9月7日

    開催地:秋田  

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  • イネの根におけるケイ酸吸収関連遺伝子の分布と発現解析

    *山地直樹、馬建鋒

    日本土壌肥料学会 

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    開催年月日: 2006年9月5日 - 2006年9月7日

    開催地:秋田  

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  • Functional analysis of HvYS1 in barley.

    Ma, J. F., Ueno, D., *Yamaji, N. and Murata, Y.

    13th International Symposium Iron Nutrition and Interactions in Plants. 

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    開催年月日: 2006年7月3日 - 2006年7月7日

    開催地:Montpellier, France  

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  • Spatial distribution and temporal variation of rice Si transporter Lsi1.

    *Yamaji, N. and Ma, J. F.

    Workshop on Silicon in Plants. 

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    開催年月日: 2006年5月25日

    開催地:Kurashiki, Japan  

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  • Cloning and Characterization of a Rice Al-tolerant Gene Als1.

    Huang C., *Yamaji N. and Ma J. F.

    日本植物生理学会 

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    開催年月日: 2006年3月19日 - 2006年3月21日

    開催地:つくば  

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  • ケイ酸吸収遺伝子Lsi1の機能解析

    三谷 奈見季、*山地 直樹、玉井 一規、小西 左江子、矢野 昌裕、馬 建鋒

    日本植物生理学会 

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    開催年月日: 2006年3月19日 - 2006年3月21日

    開催地:つくば  

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  • イネケイ酸吸収関連遺伝子Lsi2 の機能解析

    *山地 直樹、馬 建鋒

    日本植物生理学会 

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    開催年月日: 2006年3月19日 - 2006年3月21日

    開催地:つくば  

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  • OsPHO1;3 mediates redistribution of phosphorus in rice.

    Che, J, Qu, Y. T, Mitani-Ueno, N, Yamaji, N, Shen, R. F, Ma, J. F

    The 11th International Symposium on Plant-Soil Interactions at Low pH (PSILPH)  2023年10月19日 

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  • Functional characterization of two novel phosphorus transporters in barley.

    Huang, H. L, Gu, M, Hisano, H, Yamaji, N, Mitani-Ueno, N, Huang, S. and, Ma, J. F

    The 11th International Symposium on Plant-Soil Interactions at Low pH (PSILPH)  2023年10月18日 

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  • OsOPT7 mediates xylem unloading of Fe for its preferential distribution in rice.

    Yamaji, N, Yoshioka, Y, Huang, S, Miyaji, T, Sasaki, A, Ma, J. F

    The 11th International Symposium on Plant-Soil Interactions at Low pH (PSILPH)  2023年10月17日 

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  • HvSPDT-L is probably involved in redistribution of phosphorus in barley

    Huang, H, Hisano, H, Yamaji, N, Mitani-Ueno, N, Sato, K, Ma, J. F

    3rd Barley Mutant Conference  2023年10月9日 

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  • ケイ素応答性長距離シグナルの同定

    三谷奈見季, 山地直樹, 小西範幸, 馬 建鋒

    日本土壌肥料学会2023年度愛媛大会  2023年9月13日 

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  • イネのケイ酸チャネルLsi6の変異体を用いたケイ素吸収・分配制御の詳細解析

    山地直樹, 櫻井 玄, 三谷奈見季, 黄 勝, 馬 建鋒

    日本土壌肥料学会2023年度愛媛大会  2023年9月13日 

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  • Local distribution of manganese to leaf sheath is mediated by OsNramp5 in rice

    Huang, S, Yamaji, N, Konishi, N, Ma, J. F

    19th International Workshop on Plant Membrane Biology  2023年3月28日 

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  • A transporter essential for proper deposition of Si in rice

    Mitani-Ueno, N, Yamaji, N, Huang, S, Yoshioka, Y, Miyaji, T, Ma, J. F

    19th International Workshop on Plant Membrane Biology  2023年3月28日 

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  • A K+/Ca2+ transporter OsSKOR is involved in Ca accumulation in rice grain

    Wang, P, Yamaji, N, Mitani-Ueno, N, Konishi, N, Ma, J. F

    19th International Workshop on Plant Membrane Biology  2023年3月28日 

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  • イネのホウ酸吸収と根の形態及び構造との関連

    藤井理樹, 山地直樹, 馬 建鋒

    第63回日本植物生理学会年会  2023年3月16日 

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  • Functional characterization of two genes involved in phosphorus loading into barley grain

    第63回日本植物生理学会年会  2023年3月16日 

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  • OsMGT2 mediates the translocation and preferential distribution of magnesium in rice

    第63回日本植物生理学会年会  2023年3月16日 

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  • イネのケイ素吸収を制御する長距離シグナルタンパク質の同定

    山地直樹, 三谷奈見季, 小西範幸, 馬 建鋒

    日本植物生理学会年会  2023年3月15日 

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  • Role of ZIP family transporters in zinc uptake and distribution in rice

    Huang, S, Mu, S, Yamaji, N, Zheng, L. Q, Ma, J. F

    54th Annual Conference of the German Society of Plant Nutrition, From Molecules to Traits – How Plants Feed the Future  2022年10月5日 

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  • イネ科植物の酸性土壌適応メカニズムとその進化的考察 招待

    山地直樹

    日本生化学会  2021年11月 

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    会議種別:シンポジウム・ワークショップ パネル(公募)  

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  • Role of OsLsi6 in B distribution under B-sufficient condition in rice

    黄 勝, 山地直樹, 三谷奈見季, 馬 建鋒

    日本土壌肥料学会  2021年9月 

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    会議種別:口頭発表(一般)  

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  • ケイ素蓄積に伴うイネ葉身のトランスクリプトーム解析

    山地直樹, 三谷奈見季, 馬 建鋒

    日本土壌肥料学会  2021年9月 

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    会議種別:ポスター発表  

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  • イネのケイ素蓄積に関わる輸送体SIET4の更なる解析

    三谷奈見季, 山地直樹, 馬 建鋒

    日本土壌肥料学会  2021年9月 

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    会議種別:ポスター発表  

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  • イネにおけるホウ素吸収の制御機構

    黄 勝, 山地直樹, 小西範幸, 邵 継鋒, 馬 建鋒

    日本土壌肥料学会  2021年9月 

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    会議種別:口頭発表(一般)  

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  • A glycosyltransferase gene is required for tolerance to acidic stress in rice

    王 珮同, 山地直樹, 馬 建鋒

    日本土壌肥料学会  2021年9月 

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    会議種別:口頭発表(一般)  

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  • Role of lateral roots and root hairs in high uptake of manganese and cadmium in rice

    余 恩, 山地直樹, 馬 建鋒

    日本土壌肥料学会  2021年9月 

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    会議種別:口頭発表(一般)  

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  • イネOsBOR1は器官・組織毎のホウ素分配に関与する

    Shao, J. F, 山地直樹, Huang, S, 馬 建鋒

    日本植物生理学会年会  2021年3月 

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    会議種別:口頭発表(一般)  

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  • イネにおける細胞壁多糖類のアルミニウム適応応答

    長山照樹, 斉藤大夢, 中村敦子, 山地直樹, 佐藤 忍, 古川 純, 岩井宏暁

    日本植物生理学会年会  2021年3月 

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    会議種別:口頭発表(一般)  

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▼全件表示

共同研究・競争的資金等の研究

  • 土壌環境変動に応答する植物のミネラル輸送システムの可塑性の解明

    研究課題/領域番号:21H05034  2021年07月 - 2026年03月

    日本学術振興会  科学研究費助成事業 基盤研究(S)  基盤研究(S)

    馬 建鋒, 山地 直樹, 宮地 孝明, 三谷 奈見季, 菅 倫寛

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    配分額:189280000円 ( 直接経費:145600000円 、 間接経費:43680000円 )

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  • イネ科作物の有害ミネラル集積機構の解明

    研究課題/領域番号:21H04716  2021年04月 - 2025年03月

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    馬 建鋒, 山地 直樹, 三谷 奈見季

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    配分額:41860000円 ( 直接経費:32200000円 、 間接経費:9660000円 )

    1.イネカドミウム集積とヒ素集積 QTL 遺伝子のマッピング
    イネのカドミウム品種間差を利用して作出したマッピング集団を用いて、新しいマーカーを作成しながら、染色体7番と11番のQTL遺伝子の候補領域を狭めることができた。7番染色体にあるQTL遺伝子についてプロモーター領域配列の比較、発現パータンの比較などを行い、この遺伝子の発現の違いがカドミウム集積に寄与している可能性を示した。またカドミウム集積における側根と根毛の役割を解析し、根毛ではなく、側根がカドミウムの吸収に大きな役割を果たしていることを明らかにした。
    ヒ素集積に関して、6番染色体にあるヒ素集積に関与するQTL遺伝子の候補領域を350kbまで狭めることができた。
    2.オオムギのカドミウム集積QTL遺伝子のマッピングとヒ素集積の品種間差
    オオムギのカドミウム集積の品種間差を利用して、4番染色体にあるカドミウム集積に関与するQTL遺伝子のマッピングを行った。いくつかのマーカーを新たに設計し、候補領域を8375kbまで狭めることができた。
    またオオムギヒ素集積の品種間差を調べるために、コアコレクションから100品種を選んで、圃場栽培された種子とわら中のヒ素濃度を測定し、比較した。その結果、ヒ素集積において大きな品種間差があることが分かった。また水耕栽培で幼苗のヒ素集積も比較し、種子のヒ素集積と必ず一致しないことを明らかにした。いくつか集積が大きく異なる品種を選んで、関連遺伝子をマッピングするための交配を行った。

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  • 根圏生態系の季節変動から紐解く二毛作体系の生物学的な持続性

    研究課題/領域番号:19KT0011  2019年07月 - 2022年03月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    谷 明生, 山本 敏央, 山地 直樹, 山下 純, 門田 有希, 中川 智行, 最相 大輔, 持田 恵一

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    配分額:18590000円 ( 直接経費:14300000円 、 間接経費:4290000円 )

    植物の生育は植物の遺伝背景や環境要因等の相互作用の結果として捉えることができる。根圏微生物叢は植物の生育に多大な影響を与えており、逆に、植物の遺伝的背景や環境要因にも規定され、相互作用すると考えられる。本研究の目的は、圃場環境において微生物叢を含むあらゆるパラメータをデータ化し、それらの要因間のネットワークを可視化し,農業生態系としての季節変動動態を理解することである。
    研究所実験圃場の慣行区と無施肥区において、アルミニウムに対する感受性の異なるイネとオオムギの品種対を対象に、隔週で各条件3植物個体ずつ(イネは6個体)サンプリングした。環境要因として、フィールドサーバーを用いて日照、降雨、温度、湿度、風向、風速を測定した。土壌環境データとして根圏土壌を採取し、ICP-MSによる水抽出可能な元素の組成を測定し、土壌センサを用いて土壌pHを測定した。さらに土壌の根圏微生物DNAを精製し、16S rRNA遺伝子アンプリコンシーケンスを行った。
    施肥の有無によりイオン等の動態は変化し、pHは慣行区で低く、カリウム、リンは慣行区で高濃度であるが早い時期に消費された。慣行区の硫黄、無施肥区のマグネシウム、マンガン、銅が落水により増加した。同じく落水によると考えられるヒ素の増加、カドミウムの低下が無施肥区で見られた。
    微生物叢は、イネではMassilia属、Pseudomonadaceae、Oxalobacteraceaeがstar1で多い傾向が見られた。オオムギでは根圏土壌と根のサンプルで相違が見られ、根のサンプルにのみJanthinobacterium, Methylibium属細菌が多く存在することが分かった。これらはオオムギ根のエンドファイトであると考えられ、そのオオムギに対する共生機構や成長への影響に興味が持たれる。

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  • イネの環境応答統合メカニズムにおける節の役割

    研究課題/領域番号:19H03250  2019年04月 - 2023年03月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    山地 直樹, 三谷 奈見季, 横正 健剛

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    配分額:17290000円 ( 直接経費:13300000円 、 間接経費:3990000円 )

    イネ科植物は「節」を構成単位とするモジュール化された体制を採用しており、節には栄養素の分配や成長/休眠の制御などの機能が高度に集約されていることから、個体レベルの環境応答においても様々なシグナルの受発信/変換/転送を担う主要な情報処理の場であると考えられる。
    本年度は、節の環境応答に関与する遺伝子を見出すため、出穂期のイネに対し、短期的なストレス処理を行った最上位節(節I)と下位節(節V~VI)の次世代シーケンサーを用いたRNA-seq解析を実施した。各処理区で発現が有意に変動した転写調節因子、受容体、ペプチドホルモン、などをコードする数十の機能未知遺伝子を選抜した(一次選抜)。さらに定量的RT- PCRによるより詳細な遺伝子発現解析を行い、既存の発現データベース等の情報も加味して検討した結果、節において他の器官よりも特に発現レベルが高く、いずれかのストレス処理に顕著に応答する13の遺伝子を選抜した(二次選抜)。これらの遺伝子についてCRISPR/Cas9法による遺伝子破壊株の作出を進めている。
    イネ節の肥大維管束木部転送細胞に極めて高発現し、ケイ酸の分配を制御する輸送体Lsi6の遺伝子発現を制御する転写調節因子の探索については、酵母one-hybridシステムによるスクリーニングを計画していたが、Lsi6プロモーターの候補領域が酵母において著しい偽陽性を生じることが判明した。
    イネのアルミニウム耐性遺伝子でバクテリア型ABC輸送体のサブユニットをコードするSTAR1については節の維管束系でも高発現していることなどが確認できた。

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  • 作物のミネラル輸送システムの統合解析

    研究課題/領域番号:16H06296  2016年04月 - 2021年03月

    日本学術振興会  科学研究費助成事業 特別推進研究  特別推進研究

    馬 建鋒, 山地 直樹, 宮地 孝明, 三谷 奈見季, 横正 健剛, 菅 倫寛, 櫻井 玄

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    配分額:536250000円 ( 直接経費:412500000円 、 間接経費:123750000円 )

    イネのケイ素輸送体OsSIET3とOsSIET5,リン輸送体OsSULTR3;3、鉄輸送体OsOPT7、ムギネ酸輸送体OsVMT、ソバの金属輸送体Nramp5などの発現パターン、組織局在、輸送活性及び変異体の表現型を解析し、それぞれミネラルの輸送に果たす役割を明らかにした。そのうち、OsVMTはイネ節の維管束間の柔組織に発現し、鉄および亜鉛の維管束間輸送過程に関与し、その変異体では結果的に精白米中の鉄および亜鉛の蓄積が1.5~2倍に増加したことを明らかにした。 またイネのカスパリー帯形成に必要な遺伝子OsCASP1を同定し、ミネラルの選択的吸収におけるカスパリー帯の重要性を解明した。さらにリン酸輸送体AtSPDTの解析を通じて、双子葉植物がイネ科植物と異なるミネラルの分配機構を持つことを突き止めた。
    導入したLA-ICP-MSを用いて、植物サンプルの元素イメージング法を確立した 。この方法は根、葉、節など様々な植物組織上で、細胞/組織レベルの元素分布を可視化でき、多元素や同位体も同時に検出できる。また定量的イメージング法も開発した。
    ミネラル輸送体の結晶構造に関して、ケイ酸輸送体、3種類の金属輸送体、アニオン小分子輸送体の大量発現・精製・結晶化・機能解析を推進した。ケイ酸輸送体はH30年度に解析した立体構造に基づいて機能解析を終了した。また1種類の金属輸送体、アニオン小分子輸送体は低分解能データが得られた。さらにその他2種類の金属輸送体についても精製条件を検討して安定な状態で精製できるようになった。
    イネ全体におけるトランスポータ配置の最適性解析を行い、その生物学的な意義を検証した。イネが有害な物質を若い器官に蓄積しないために、どのようなトランスポーター配置を行うべきなのかなどをモデル解析で示す例示研究を行った。

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  • イネ科植物の土壌pH適応におけるトレードオフ

    研究課題/領域番号:16K14871  2016年04月 - 2017年03月

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    山地 直樹, 馬 建鋒, 三谷 奈見季, 横正 健剛

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    配分額:3770000円 ( 直接経費:2900000円 、 間接経費:870000円 )

    土壌pHは植物の栄養素の獲得に大きな影響を及ぼす。酸性土壌では、主に土壌から溶け出すアルミニウムイオンによって植物の生長が阻害される。アルカリ土壌では、主に鉄が不溶化し植物は鉄欠乏となって生育が悪化する。植物種によって土壌pHへの適応性は大きく異なるが、その種間差を規定する分子メカニズムは解明されていない。本研究では土壌pH適応性が大きく異なる3種のイネ科作物イネ(野生型、star1 変異体、art1変異体)・オオムギ・ライムギを用い、3つの条件、Alストレス条件(pH 4.5)、通常条件(pH6.0)、鉄欠乏条件(pH7.5)で根の比較トランスクリプトーム解析を実施した。

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  • イネの有害ミネラル集積を制御する因子の同定

    研究課題/領域番号:16H02540  2016年04月 - 2017年03月

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    馬 建鋒, 山地 直樹, 三谷 奈見季

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    配分額:40560000円 ( 直接経費:31200000円 、 間接経費:9360000円 )

    これまでにイネのカドミウム集積の品種間差を利用して、幾つかカドミウム集積に関与する新規QTLを検出している。そのうちの一つ、7番染色体に座乗するqCd7についてファインマッピングを行い、候補遺伝子の絞込みに成功した。この遺伝子の発現をカドミウム集積量の異なる両親間で比較すると、差が認められなかった。またこの遺伝子はカドミウムによる誘導がなかった。両親間の配列を比較すると、1塩基の違いによる1アミノ酸の変化があった。しかし、酵母を用いたアッセイでは、両アリルともカドミウムに対する輸送活性を示した。両親のイネを用いて生理学的な解析も行った。その結果、カドミウム高集積品種では、マンガンによるカドミウムの抑制効果が見られたが、カドミウム低集積品種では、この抑制効果はほとんど見られなかった。これらの結果から、この1アミノ酸の変化はカドミウム輸送に対する親和性が低くなっている可能性があり、現在更なる解析を行っている。
    ヒ素に関しては、ヒ素集積の異なる3種類のイネを用いて、関連遺伝子の発現量を比較した。その結果、ヒ素低集積品種のOsLsi1とOsLsi2の発現量が高集積品種より低いことが分かった。またケイ酸の吸収量も低くなっていた。これらのことはヒ素の低集積がケイ酸輸送体遺伝子の低発現に起因することを示唆している。

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  • 栄養素分配における イネ節の機能解明

    研究課題/領域番号:15H04469  2015年04月 - 2018年03月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    山地 直樹, 三谷 奈見季, 馬 建鋒

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    配分額:16900000円 ( 直接経費:13000000円 、 間接経費:3900000円 )

    イネ科植物の節は著しく発達した維管束群が高度に組織化されており、栄養素の“維管束間輸送”によってその分配を制御していることが次第に明らかになってきた。本研究ではイネの節においてリン、ホウ素、鉄の維管束間輸送と器官間の分配に関わる新奇輸送体を同定し、その生理機能解析を行った。このうちリンの新規輸送体SPDTは節の維管束木部などに発現し、リンの維管束間輸送を促進することで新葉や種子へのリンの優先的な分配に寄与する。
    また節の肥大維管束木部転送細胞で高発現するケイ酸輸送体Lsi6のプロモーター解析を行い、組織特異的な遺伝子発現を制御する領域を推定した。

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  • イネの有害元素集積を制御する遺伝子の同定と応用

    研究課題/領域番号:24248014  2012年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    馬 建鋒, 山地 直樹, 三谷 奈見季

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    配分額:44850000円 ( 直接経費:34500000円 、 間接経費:10350000円 )

    イネのカドミウムやヒ素の集積に関与する新規遺伝子の同定を行った。カドミウムの集積に関して、カドミウム吸収の主要輸送体OsNramp5を同定した。OsNramp5は根の外皮と内皮細胞の遠心側に偏在していた。また液胞膜に局在するカドミウム輸送体遺伝子OsHMA3を過剰発現させると、カドミウムの耐性も増加した。さらにカドミウムの種子への分配には節で発現しているOsHMA2が関与していた。一方、ヒ素の集積に関して、OsABCC1が種子へのヒ素の集積を抑制していることを明らかにした。OsABCC1は主に節の篩部伴細胞の液胞膜に局在していた。

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  • イネの節における選択的ミネラル分配機構の解明

    研究課題/領域番号:23688009  2011年04月 - 2014年03月

    日本学術振興会  科学研究費助成事業 若手研究(A)  若手研究(A)

    山地 直樹

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    配分額:26390000円 ( 直接経費:20300000円 、 間接経費:6090000円 )

    イネ科植物は「節」において複数の維管束系を著しく発達させ、根から吸収した無機栄養素の新葉や種子への優先的な分配を実現しているが、その分子機構は解明されていなかった。本研究ではイネ節で働く亜鉛、銅、マンガンの輸送体タンパク質を同定し、元素毎に異なる特徴的な分配様式に大きく寄与していることを明らかにした。これらはイネ科植物の生長戦略上の重要な新知見であるとともに、穀物の生産性・安全性・栄養価の向上に応用できる。

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  • 劣悪化する土壌環境に適応するための植物の知恵

    研究課題/領域番号:22119002  2010年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    馬 建鋒, 山地 直樹, 藤原 徹

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    配分額:241540000円 ( 直接経費:185800000円 、 間接経費:55740000円 )

    イネの高アルミニウム耐性に関して複数の耐性遺伝子を同定した。またこれら耐性遺伝子の発現を制御する転写因子ART1のシス配列を同定した。オオムギのアルミニウム耐性遺伝子HvAACTの発現は上流への1kbの挿入で、シラゲガヤのアルミニウム耐性遺伝子HlALMT1の発現はプロモーター領域にART1と結合するシス因子の数を増やすことによって耐性を向上させたことを明らかにした。さらに環境中のマンガン濃度の変動に対処するために、イネの節で発現しているOsNramp3がマンガンの分配をコントロールしていることを解明した。そのほかにミネラル輸送に関する遺伝子を複数同定し、ケイ素吸収の数理モデルを構築した。

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  • アルミニウム結合性ペプチドの同定

    研究課題/領域番号:22658022  2010年 - 2011年

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    馬 建鋒, 山地 直樹

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    配分額:3650000円 ( 直接経費:3200000円 、 間接経費:450000円 )

    イネのアルミニウム耐性転写調節因子ART1の制御下にある遺伝子OsCDT3の機能解析を行った。OsCDT3は僅か53アミノ酸のペプチドをコードし、そのうち14個のアミノ酸はシステインであった。OsCDT3は主に根で発現し、アルミニウムによって誘導される。OsCDT3は細胞膜に局在していた。OsCDT3の発現を抑制すると、アルミニウム耐性が低下した。また酵母で発現させると、アルミニウム耐性が高くなり、細胞内のアルミニウム濃度が減少した。これらのことからOsCDT3はアルミニウムを細胞内に輸送しないようにアポプラストで無毒化している可能性がある。

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  • 植物の新規有害金属トランスポーターの同定

    研究課題/領域番号:21248009  2009年 - 2011年

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    馬 建鋒, 山地 直樹

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    配分額:48620000円 ( 直接経費:37400000円 、 間接経費:11220000円 )

    植物の有害金属(カドミウム、ヒ素、アルミニウム)の集積及び耐性に関わるトランスポーター遺伝子の同定を行った。カドミウムの超集積植物からカドミウムの無毒化に必要なTcHMA3、イネのカドミウムの集積に関わるOsHMA3をそれぞれ同定した。イネの内向きケイ酸トランスポーターLsi1はメチル化されたヒ素(MMAとDMA)をも輸送することを明らかにした。さらにソバ、シロイヌナズナ及びイネからアルミニウムの耐性に関わるいくつかの新規トランスポーター遺伝子を同定した。

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  • イネのアルミニウム応答性遺伝子発現機構の解析

    研究課題/領域番号:21780057  2009年 - 2010年

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    山地 直樹

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    配分額:4550000円 ( 直接経費:3500000円 、 間接経費:1050000円 )

    酸性土壌におけるアルミニウムイオン毒性に対して、イネではAl耐性遺伝子群の発現が誘導される。本研究ではイネ新規転写調節因子ART1がAl耐性遺伝子STAR1のプロモーター領域に結合してAl応答を制御していることを明らかにし、そのシス配列を決定した。また、ART1遺伝子を高発現する形質転換イネを作成し、ART1およびSTAR1などの耐性遺伝子群の発現の著しい増大を確認したが、高いAl感受性を示した。

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  • ケイ酸トランスポーター遺伝子の単離と解析

    研究課題/領域番号:17078008  2005年 - 2009年

    日本学術振興会  科学研究費助成事業 特定領域研究  特定領域研究

    馬 建鋒, 山地 直樹

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    配分額:79500000円 ( 直接経費:79500000円 )

    ケイ素は植物の有益元素で、植物の複合ストレスを軽減することができる。本研究では、植物のケイ酸の吸収、分配に関与するトランスポーターを同定した。イネから同定したLsi1とLsi2は根で発現し、根の外皮と内皮細胞に極性をもって偏在し、根によるケイ酸の吸収に重要な役割を果たしている。またLsi6は葉の維間束木部や第I節で発現し、ケイ酸の分配に関与している。トウモロコシやカボチャからも同様のトランスポーターを同定しているが、イネと比べ、発現レベルや細胞局在性が異なっていた。

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担当授業科目

  • 植物ストレス分子生物学 (2023年度) 後期  - その他

  • 植物ストレス分子生物学 (2023年度) 後期  - その他

  • 植物ストレス制御学演習 (2023年度) 前期  - その他

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  • 植物ストレス学 (2023年度) 前期  - 水5~8

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  • 特別研究 (2023年度) その他  - その他

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  • 植物ストレス学 (2022年度) 前期  - 水5~8

  • 植物栄養学 (2022年度) 第4学期  - 水1,水2

  • 生物資源科学特別研究 (2022年度) 通年  - その他

  • 植物ストレス分子生物学 (2021年度) 後期  - その他

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  • 植物ストレス学 (2021年度) 前期  - 水5~8

  • 生物資源科学特別研究 (2021年度) 通年  - その他

  • 自然・環境科学教養・実践論 (2021年度) 後期  - 木7,木8

  • 植物ストレス分子生物学 (2020年度) 後期  - その他

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  • 植物ストレス学 (2020年度) 前期  - その他

  • 生物資源科学特別研究 (2020年度) 通年  - その他

  • 自然・環境科学教養・実践論 (2020年度) 後期  - 木7,木8

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