Updated on 2026/08/30

写真a

 
Iwane Atsuko
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Special-Appointment Professor
Position
Special-Appointment Professor
External link

Degree

  • Doctor of Science ( 1997.6   Osaka University )

  • Master of Pharmacy ( Tokyo University of Pharmacy and Life Science )

Research Interests

  • Expression

  • 情報伝達

  • イメージング

  • FIB-SEM

  • 3D-reconstruction

  • imaging

  • 筋肉

  • 発現

  • 細胞

  • cell

  • signal transduction

  • Intracellular organelle

  • Muscle

Research History

  • Okayama University   Faculty of Medicine, Dentistry and Pharmaceutical Sciences   Professor appointed with the special mission

    2026.3

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  • Yamaguchi University   Research Institute for Cell Design Medical Science   Professor appointed with the spetial mission

    2023.7 - 2026.2

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  • Hiroshima University   Research Institute for Radiation Biology and Medicine

    2021.4 - 2026.3

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  • RIKEN Center for Biosystems Dynamics Research   Laboratory for Cell Field Structure   Team Leader

    2020.4 - 2023.3

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  • Hiroshima University   Graduate School Integrated Sciences for Life   Specially Appointed Professor

    2019.4 - 2021.3

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  • Hiroshima University   大学院理学研究科   Specially Appointed Professor

    2018.11 - 2019.3

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  • RIKEN Center for Biosystems Dynamics Research   Laboratory for Cell Field Structure   Unit Leader

    2018.4 - 2020.3

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  • Hiroshima University   Graduate School of Science   Visiting professor

    2017.10 - 2018.10

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  • Osaka University   Graduate School of Frontier Biosciences   Invitation professor

    2013.4

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  • RIKEN Quantitative Biology Center   Unit Leader

    2013.4 - 2017.3

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  • Osaka University   Graduate School of Frontier Biosciences, Graduate School of Medicine   Associate Professor

    2007.11 - 2013.3

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  • Osaka University   Graduate School of Medicine   Associate Professor

    2007 - 2007.10

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  • Osaka University   Graduate School of Medicine   Associate professor

    2006 - 2007

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  • Osaka University   Graduate School of Frontier Biosciences   Research Associate

    2002 - 2006

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  • - Osaka University Graduate School of Frontier Biosciences, Research Associate

    2002

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  • Osaka University   Graduate School of Medicine   Assistant professor

    1999 - 2002

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  • Osaka University Medical School, Research Associate

    1999 - 2002

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  • Osaka University   Graduate School of Engineering Science   Assistant professor

    1997 - 1999

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  • Osaka University Faculty of Engineering Science, Research Associate

    1997 - 1999

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  • Osaka University   Graduate School of Engineering Science

    1992 - 1997

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  • Osaka University Faculty of Engineering Science, Associate Research

    1992 - 1997

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  • 株)蛋白工学研究所   研究助手

    1988 - 1991

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  • Protein Engineering Research, Research Assistant.

    1988 - 1991

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  • 財)癌研究会癌研究所   研究助手

    1984 - 1988

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  • Japanese Foundation for Cancer Research, Research Assistant

    1984 - 1988

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Professional Memberships

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Committee Memberships

  • 日本顕微鏡学会   第75回学術講演会実行委員会プログラム委員  

    2019 - 2019.6   

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    Committee type:Academic society

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  • 日本植物学会   第82回大会実行委員会プログラム担当  

    2018 - 2018.9   

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    Committee type:Academic society

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  • 大阪大学   男女協働推進連携会議委員  

    2017   

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    Committee type:Other

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  • 大阪市   特区地域進出等事業計画認定審査会委員  

    2015   

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    Committee type:Municipal

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  • 大阪府   成長産業特別集積区域進出等成長産業事業計画認定審査会委員  

    2015   

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    Committee type:Municipal

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  • 理化学研究所   男女共同参画推進委員  

    2015   

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    Committee type:Other

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Papers

  • Sexual life cycle establishes the unicellular red algae Cyanidiophyceae as a genetically tractable model lineage for eukaryotic evolution Reviewed

    Shunsuke Hirooka, Takayuki Fujiwara, Mark Seger, Soichi Inagaki, Shota Yamashita, Dai Tsujino, Ryo Onuma, Yu Kanesaki, Satoru Watanabe, Yuu Hirose, Ryudo Ohbayashi, Mari Takusagawa, Baifeng Zhou, Reiko Tomita, Fumi Yagisawa, Peter Lammers, Atsuko H. Iwane, Shin-ya Miyagishima

    2025.10

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    Publisher:Cold Spring Harbor Laboratory  

    Abstract

    The thermo-acidophilic unicellular algal class Cyanidiophyceae is the earliest-branching lineage in red algae, which diverged from Viridiplantae lineage (green algae and land plants) soon after chloroplast establishment in the common ancestor of Archaeplastida. Cyanidiophyceae possess extremely simple genomes (8.7–17.8 Mb; approximately 4,800– 7,800 genes), and the cell-wall-less, genetically tractable strain Cyanidioschyzon merolae 10D has served as a model organism. However, its unknown sexual life cycle has limited its utility in studies of evolution and genetics. Inspired by the recent discovery of sexual reproduction in the cyanidiophycean genus Galdieria, we identified similar life cycles in the other cyanidiophycean genera Cyanidioschyzon, Cyanidiococcus, and Cyanidium. In these genera, the cell-walled diploid form, exclusively observed in nature, produces a cell-wall-less haploid form when the culture pH is lowered, and both proliferate asexually. In addition, the cell-wall-less Cyanidioschyzon merolae 10D strain has been shown to be a haploid clone that forms a cell-walled diploid through mating with other haploid clones. Building on these findings, we generated high-quality genomic resources with phase-specific transcriptomes and developed genetic manipulation systems using the cell-wall-less haploids of these genera. We further uncovered phase-specific distribution of histone H3 lysine 27 trimethylation linked to haploid- and diploid-specific gene expression, including transcription factors involved in differentiation associated with sexual reproduction in plants. Additionally, biparental inheritance of organelle DNA occurs following isogamous mating of haploid cells but resolves into uniparental inheritance during diploid proliferation. These advances position Cyanidiophyceae as a powerful model lineage for studying early Archaeplastida evolution, the shared mechanisms of photosynthetic eukaryotes, and their environmental adaptation.

    DOI: 10.1101/2025.10.15.681507

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  • Immotile cilia mechanically sense the direction of fluid flow for left-right determination Reviewed

    Takanobu A. Katoh, Toshihiro Omori, Katsutoshi Mizuno, Xiaorei Sai, Katsura Minegishi, Yayoi Ikawa, Hiromi Nishimura, Takeshi Itabashi, Eriko Kajikawa, Sylvain Hiver, Atsuko H. Iwane, Takuji Ishikawa, Yasushi Okada, Takayuki Nishizaka, Hiroshi Hamada

    Science   379 ( 6627 )   66 - 71   2023.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Association for the Advancement of Science (AAAS)  

    Immotile cilia at the ventral node of mouse embryos are required for sensing leftward fluid flow that breaks left-right symmetry of the body. However, the flow-sensing mechanism has long remained elusive. In this work, we show that immotile cilia at the node undergo asymmetric deformation along the dorsoventral axis in response to the flow. Application of mechanical stimuli to immotile cilia by optical tweezers induced calcium ion transients and degradation of Dand5 messenger RNA (mRNA) in the targeted cells. The Pkd2 channel protein was preferentially localized to the dorsal side of immotile cilia, and calcium ion transients were preferentially induced by mechanical stimuli directed toward the ventral side. Our results uncover the biophysical mechanism by which immotile cilia at the node sense the direction of fluid flow.

    DOI: 10.1126/science.abq8148

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  • Life cycle and functional genomics of the unicellular red alga Galdieria for elucidating algal and plant evolution and industrial use Reviewed

    Shunsuke Hirooka, Takeshi Itabashi, Takako M. Ichinose, Ryo Onuma, Takayuki Fujiwara, Shota Yamashita, Lin Wei Jong, Reiko Tomita, Atsuko H. Iwane, Shin-ya Miyagishima

    Proceedings of the National Academy of Sciences   119 ( 41 )   2022.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Proceedings of the National Academy of Sciences  

    Sexual reproduction is widespread in eukaryotes; however, only asexual reproduction has been observed in unicellular red algae, including Galdieria , which branched early in Archaeplastida. Galdieria possesses a small genome; it is polyextremophile, grows either photoautotrophically, mixotrophically, or heterotrophically, and is being developed as an industrial source of vitamins and pigments because of its high biomass productivity. Here, we show that Galdieria exhibits a sexual life cycle, alternating between cell-walled diploid and cell wall–less haploid, and that both phases can proliferate asexually. The haploid can move over surfaces and undergo self-diploidization or generate heterozygous diploids through mating. Further, we prepared the whole genome and a comparative transcriptome dataset between the diploid and haploid and developed genetic tools for the stable gene expression, gene disruption, and selectable marker recycling system using the cell wall–less haploid. The BELL/KNOX and MADS-box transcription factors, which function in haploid-to-diploid transition and development in plants, are specifically expressed in the haploid and diploid, respectively, and are involved in the haploid-to-diploid transition in Galdieria , providing information on the missing link of the sexual life cycle evolution in Archaeplastida. Four actin genes are differently involved in motility of the haploid and cytokinesis in the diploid, both of which are myosin independent and likely reflect ancestral roles of actin. We have also generated photosynthesis-deficient mutants, such as blue-colored cells, which were depleted in chlorophyll and carotenoids, for industrial pigment production. These features of Galdieria facilitate the understanding of the evolution of algae and plants and the industrial use of microalgae.

    DOI: 10.1073/pnas.2210665119

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  • Immotile cilia of the mouse node sense a fluid flow–induced mechanical force for left-right symmetry breaking Reviewed

    Takanobu A. Katoh, Toshihiro Omori, Katsutoshi Mizuno, Xiaorei Sai, Katsura Minegishi, Yayoi Ikawa, Hiromi Nishimura, Takeshi Itabashi, Eriko Kajikawa, Sylvain Hiver, Atsuko H. Iwane, Takuji Ishikawa, Yasushi Okada, Takayuki Nishizaka, Hiroshi Hamada

    2022.4

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    Publisher:Cold Spring Harbor Laboratory  

    Immotile cilia of crown cells at the node of mouse embryos are required for sensing of a leftward fluid flow1 that gives rise to the breaking of left-right (L-R) symmetry2. The flow-sensing mechanism has long remained elusive, however, with both mechanosensing and chemosensing models having been proposed1, 3–5. Here we show that immotile cilia at the mouse node respond to mechanical force. In the presence of a leftward flow, immotile cilia on the left side of the node bend toward the ventral side whereas those on the right side bend toward the dorsal side. Application of mechanical stimuli to immotile cilia along the dorsoventral axis by optical tweezers induced Ca2+ transients and degradation of Dand5 mRNA—the first known L-R asymmetric molecular events—in the targeted cells. The Pkd2 channel protein was found to be preferentially localized to the dorsal side of immotile cilia on both left and right sides of the node, and the observed induction of Ca2+ transients preferentially by mechanical stimuli directed toward the ventral side could explain the differential response of immotile cilia to the directional flow. Our results thus suggest that immotile cilia at the node sense the direction of fluid flow in a manner dependent on a flow-generated mechanical force.

    DOI: 10.1101/2022.04.11.487968

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  • ペルオキシソームを介した繊毛へのコレステロール輸送機構

    宮本 達雄, 細羽 康介, 板橋 岳志, 岩根 敦子, 阿久津 シルビア夏子, 落合 博, 斎藤 裕見子, 山本 卓, 松浦 伸也

    日本生化学会大会プログラム・講演要旨集   94回   [1T14a - 451)]   2021.11

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    Language:Japanese   Publisher:(公社)日本生化学会  

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  • The Physical Role of Mesenchymal Cells Driven by the Actin Cytoskeleton Is Essential for the Orientation of Collagen Fibrils in Zebrafish Fins Reviewed

    Junpei Kuroda, Takeshi Itabashi, Atsuko H. Iwane, Toshihiro Aramaki, Shigeru Kondo

    Frontiers in Cell and Developmental Biology   8   2020.10

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    Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media SA  

    DOI: 10.3389/fcell.2020.580520

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  • Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry. Reviewed International journal

    Katsutoshi Mizuno, Kei Shiozawa, Takanobu A Katoh, Katsura Minegishi, Takahiro Ide, Yayoi Ikawa, Hiromi Nishimura, Katsuyoshi Takaoka, Takeshi Itabashi, Atsuko H Iwane, Junichi Nakai, Hidetaka Shiratori, Hiroshi Hamada

    Science advances   6 ( 30 )   eaba1195   2020.7

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    Immotile cilia sense extracellular signals such as fluid flow, but whether Ca2+ plays a role in flow sensing has been unclear. Here, we examined the role of ciliary Ca2+ in the flow sensing that initiates the breaking of left-right (L-R) symmetry in the mouse embryo. Intraciliary and cytoplasmic Ca2+ transients were detected in the crown cells at the node. These Ca2+ transients showed L-R asymmetry, which was lost in the absence of fluid flow or the PKD2 channel. Further characterization allowed classification of the Ca2+ transients into two types: cilium-derived, L-R-asymmetric transients (type 1) and cilium-independent transients without an L-R bias (type 2). Type 1 intraciliary transients occurred preferentially at the left posterior region of the node, where L-R symmetry breaking takes place. Suppression of intraciliary Ca2+ transients delayed L-R symmetry breaking. Our results implicate cilium-derived Ca2+ transients in crown cells in initiation of L-R symmetry breaking in the mouse embryo.

    DOI: 10.1126/sciadv.aba1195

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  • Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome. Reviewed International journal

    Tatsuo Miyamoto, Kosuke Hosoba, Takeshi Itabashi, Atsuko H Iwane, Silvia Natsuko Akutsu, Hiroshi Ochiai, Yumiko Saito, Takashi Yamamoto, Shinya Matsuura

    The EMBO journal   39 ( 12 )   e103499   2020.6

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    Primary cilia are antenna-like organelles on the surface of most mammalian cells that receive sonic hedgehog (Shh) signaling in embryogenesis and carcinogenesis. Cellular cholesterol functions as a direct activator of a seven-transmembrane oncoprotein called Smoothened (Smo) and thereby induces Smo accumulation on the ciliary membrane where it transduces the Shh signal. However, how cholesterol is supplied to the ciliary membrane remains unclear. Here, we report that peroxisomes are essential for the transport of cholesterol into the ciliary membrane. Zellweger syndrome (ZS) is a peroxisome-deficient hereditary disorder with several ciliopathy-related features and cells from these patients showed a reduced cholesterol level in the ciliary membrane. Reverse genetics approaches revealed that the GTP exchange factor Rabin8, the Rab GTPase Rab10, and the microtubule minus-end-directed kinesin KIFC3 form a peroxisome-associated complex to control the movement of peroxisomes along microtubules, enabling communication between peroxisomes and ciliary pocket membranes. Our findings suggest that insufficient ciliary cholesterol levels may underlie ciliopathies.

    DOI: 10.15252/embj.2019103499

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  • Prediction of Sequential Organelles Localization under Imbalance using A Balanced Deep U-Net. Reviewed International journal

    Novanto Yudistira, Muthusubash Kavitha, Takeshi Itabashi, Atsuko H Iwane, Takio Kurita

    Scientific reports   10 ( 1 )   2626 - 2626   2020.2

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    Assessing the structure and function of organelles in living organisms of the primitive unicellular red algae Cyanidioschyzon merolae on three-dimensional sequential images demands a reliable automated technique in the class imbalance among various cellular structures during mitosis. Existing classification networks with commonly used loss functions were focused on larger numbers of cellular structures that lead to the unreliability of the system. Hence, we proposed a balanced deep regularized weighted compound dice loss (RWCDL) network for better localization of cell organelles. Specifically, we introduced two new loss functions, namely compound dice (CD) and RWCD by implementing multi-class variant dice and weighting mechanism, respectively for maximizing weights of peroxisome and nucleus among five classes as the main contribution of this study. We extended the Unet-like convolution neural network (CNN) architecture for evaluating the ability of our proposed loss functions for improved segmentation. The feasibility of the proposed approach is confirmed with three different large scale mitotic cycle data set with different number of occurrences of cell organelles. In addition, we compared the training behavior of our designed architectures with the ground truth segmentation using various performance measures. The proposed balanced RWCDL network generated the highest area under the curve (AUC) value in elevating the small and obscure peroxisome and nucleus, which is 30% higher than the network with commonly used mean square error (MSE) and dice loss (DL) functions. The experimental results indicated that the proposed approach can efficiently identify the cellular structures, even when the contour between the cells is obscure and thus convinced that the balanced deep RWCDL approach is reliable and can be helpful for biologist to accurately identify the relationship between the cell behavior and structures of cell organelles during mitosis.

    DOI: 10.1038/s41598-020-59285-9

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  • Multiplexed 129Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells. Reviewed International journal

    Ryota Mizushima, Kanako Inoue, Hideaki Fujiwara, Atsuko H Iwane, Tomonobu M Watanabe, Atsuomi Kimura

    Contrast media & molecular imaging   2020   5425934 - 5425934   2020

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    Language:English   Publishing type:Research paper (scientific journal)  

    Gas vesicle nanoparticles (GVs) are gas-containing protein assemblies expressed in bacteria and archaea. Recently, GVs have gained considerable attention for biotechnological applications as genetically encodable contrast agents for MRI and ultrasonography. However, at present, the practical use of GVs is hampered by a lack of robust methodology for their induction into mammalian cells. Here, we demonstrate the genetic reconstitution of protein nanoparticles with characteristic bicone structures similar to natural GVs in a human breast cancer cell line KPL-4 and genetic control of their size and shape through expression of reduced sets of humanized gas vesicle genes cloned into Tol2 transposon vectors, referencing the natural gas vesicle gene clusters of the cyanobacteria planktothrix rubescens/agardhii. We then report the utility of these nanoparticles as multiplexed, sensitive, and genetically encoded contrast agents for hyperpolarized xenon chemical exchange saturation transfer (HyperCEST) MRI.

    DOI: 10.1155/2020/5425934

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  • Cyanidioschyzon merolae aurora kinase phosphorylates evolutionarily conserved sites on its target to regulate mitochondrial division. Reviewed

    Shoichi Kato, Erika Okamura, Tomoko M. Matsunaga, Minami Nakayama, Yuki Kawanishi, Takako Ichinose, Atsuko H. Iwane, Takuya Sakamoto, Yuuta Imoto, Mio Ohnuma, Yuko Nomura, Hirofumi Nakagami, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, Sachihiro Matunaga

    Commun. Biol   2 ( 1 )   477 - 485   2019.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>The mitochondrion is an organelle that was derived from an endosymbiosis. Although regulation of mitochondrial growth by the host cell is necessary for the maintenance of mitochondria, it is unclear how this regulatory mechanism was acquired. To address this, we studied the primitive unicellular red alga <italic>Cyanidioschyzon merolae</italic>, which has the simplest eukaryotic genome and a single mitochondrion. Here we show that the <italic>C. merolae</italic> Aurora kinase ortholog CmAUR regulates mitochondrial division through phosphorylation of mitochondrial division ring components. One of the components, the Drp1 ortholog CmDnm1, has at least four sites phosphorylated by CmAUR. Depletion of the phosphorylation site conserved among eukaryotes induced defects such as mitochondrial distribution on one side of the cell. Taken together with the observation that human Aurora kinase phosphorylates Drp1 in vitro, we suggest that the phosphoregulation is conserved from the simplest eukaryotes to mammals, and was acquired at the primitive stage of endosymbiosis.

    DOI: 10.1038/s42003-019-0714-x

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    Other Link: http://www.nature.com/articles/s42003-019-0714-x

  • Roles of basal keratinocytes in actinotrichia formation. Reviewed International journal

    Kuroda J, Iwane AH, Kondo S

    Mechanisms of development   153   54 - 63   2018.10

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    The embryonic fins and the tip of adult fins of teleost fish are supported by rows of straight, unmineralized fibrils called actinotrichia. The proximal ends of the actinotrichia are bundled and the mineralized bones called lepidotrichia are made along them. Since malformation in actinotrichia causes wavy fin bones, the correct configuration of actinotrichia is essential for the correct construction of the fin shape. Past studies suggested that two types of cells, basal keratinocytes, and mesenchymal cells involve in the formation of actinotrichia. However, the mechanism how these cells contribute is unknown. In this study, we elucidated the role of basal keratinocytes in actinotrichia formation. First, we developed the imaging tool that specifically visualizes the basal keratinocytes and actinotrichia. Then, we established the in vitro culture method of the basal keratinocytes and found that the keratinocytes developed fine needle-like structures in it. The TEM image of them showed the specific shadow pattern of actinotrichia, indicating that the fine needle-like structures are the newly made actinotrichia. Finally, we cultured the basal keratinocytes with mature actinotrichia and observed that the basal keratinocytes actively holded actinotrichia with their membrane, and often generated a linear array of cells holding a single actinotrichium. This behavior suggested a mechanism with which long actinotrichia are made by relatively small cells. Our results clarified the role of basal keratinocyte and provided a novel insight into understanding the mechanism of actinotrichia formation.

    DOI: 10.1016/j.mod.2018.08.010

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  • Cytological analyses by advanced electron microscopy Reviewed

    Takako M. Ichinose, Atsuko H. Iwane

    Cyanidioschyzon merolae: A New Model Eukaryote for Cell and Organelle Biology   129 - 151   2018.3

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    Language:English   Publishing type:Part of collection (book)   Publisher:Springer Singapore  

    DOI: 10.1007/978-981-10-6101-1_9

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  • 3D-Visualization of the Precise Location of Symbiotic Organelle Crosstalk Throughout Mitosis in the Primitive Unicellular Eukaryotic Cell, C. Merolae

    Takako Ichinose, Keisuke Ohta, Atsuko H. Iwane

    BIOPHYSICAL JOURNAL   112 ( 3 )   576A - 576A   2017.2

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  • 3D Microstructural Visualization of the Simplest of Eukaryotic Cell (Cyanidioschyzon Merolae) during Mitosis Process using Several New Microscopic Techniques

    Atsuko H. Iwane, Keisuke Ohta

    BIOPHYSICAL JOURNAL   110 ( 3 )   155A - 155A   2016.2

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  • The Role of Structural Dynamics of Actin in Class-Specific Myosin Motility Reviewed

    Taro Q. P. Noguchi, Masatoshi Morimatsu, Atsuko H. Iwane, Toshio Yanagida, Taro Q. P. Uyeda

    PLOS ONE   10 ( 5 )   e0126262   2015.5

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    DOI: 10.1371/journal.pone.0126262

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  • Local heat activation of single myosins based on optical trapping of gold nanoparticles. Reviewed International journal

    Mitsuhiro Iwaki, Atsuko H Iwane, Keigo Ikezaki, Toshio Yanagida

    Nano letters   15 ( 4 )   2456 - 61   2015.4

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    Myosin is a mechano-enzyme that hydrolyzes ATP in order to move unidirectionally along actin filaments. Here we show by single molecule imaging that myosin V motion can be activated by local heat. We constructed a dark-field microscopy that included optical tweezers to monitor 80 nm gold nanoparticles (GNP) bound to single myosin V molecules with nanometer and submillisecond accuracy. We observed 34 nm processive steps along actin filaments like those seen when using 200 nm polystyrene beads (PB) but dwell times (ATPase activity) that were 4.5 times faster. Further, by using DNA nanotechnology (DNA origami) and myosin V as a nanometric thermometer, the temperature gradient surrounding optically trapped GNP could be estimated with nanometer accuracy. We propose our single molecule measurement system should advance quantitative analysis of the thermal control of biological and artificial systems like nanoscale thermal ratchet motors.

    DOI: 10.1021/nl5049059

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  • Three-Dimensional Microstructural Visualization of Mitosis using Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) and 3Mv Ultra-High Voltage Electron Microscope (UHVEM) Tomography with Nanoscale Resolution at Whole Cell Level

    Atsuko H. Iwane, Keisuke Ohta

    BIOPHYSICAL JOURNAL   108 ( 2 )   618A - 618A   2015.1

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  • Development for dynamic live cell imaging by cryo-electron tomography and STEM.

    Iwane AH, Ogawa R, Nagai R, Kawamoto A, Aoyama K

    Biophysical Journal   104 ( 2 )   354a   2013.1

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    DOI: 10.1016/j.bpj.2012.11.1962

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  • Spontaneous Structural Changes in Actin Regulate G-F Transformation Reviewed

    Masatoshi Morimatsu, Yuichi Togashi, So Nishikawa, Mitsuhiro Sugawa, Atsuko H. Iwane, Toshio Yanagida

    PLOS ONE   7 ( 11 )   e45864   2012.11

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    DOI: 10.1371/journal.pone.0045864

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  • Simultaneous observation of the lever arm and head explains myosin VI dual function. Reviewed International journal

    Keigo Ikezaki, Tomotaka Komori, Mitsuhiro Sugawa, Yoshiyuki Arai, So Nishikawa, Atsuko H Iwane, Toshio Yanagida

    Small (Weinheim an der Bergstrasse, Germany)   8 ( 19 )   3035 - 40   2012.10

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    Myosin VI is an adenosine triphosphate (ATP)-driven dimeric molecular motor that has dual function as a vesicle transporter and a cytoskeletal anchor. Recently, it was reported that myosin VI generates three types of steps by taking either a distant binding or adjacent binding state (noncanonical hand-over-hand step pathway). The adjacent binding state, in which both heads bind to an actin filament near one another, is unique to myosin VI and therefore may help explain its distinct features. However, detailed information of the adjacent binding state remains unclear. Here simultaneous observations of the head and tail domain during stepping are presented. These observations show that the lever arms tilt forward in the adjacent binding state. Furthermore, it is revealed that either head could take the subsequent step with equal probability from this state. Together with previous results, a comprehensive stepping scheme is proposed; it includes the tail domain motion to explain how myosin VI achieves its dual function.

    DOI: 10.1002/smll.201200765

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  • G146V mutation at the hinge region of actin reveals a myosin class-specific requirement of actin conformations for motility. Reviewed International journal

    Taro Q P Noguchi, Tomotaka Komori, Nobuhisa Umeki, Noriyuki Demizu, Kohji Ito, Atsuko Hikikoshi Iwane, Kiyotaka Tokuraku, Toshio Yanagida, Taro Q P Uyeda

    The Journal of biological chemistry   287 ( 29 )   24339 - 45   2012.7

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    The G146V mutation in actin is dominant lethal in yeast. G146V actin filaments bind cofilin only minimally, presumably because cofilin binding requires the large and small actin domains to twist with respect to one another around the hinge region containing Gly-146, and the mutation inhibits that twisting motion. A number of studies have suggested that force generation by myosin also requires actin filaments to undergo conformational changes. This prompted us to examine the effects of the G146V mutation on myosin motility. When compared with wild-type actin filaments, G146V filaments showed a 78% slower gliding velocity and a 70% smaller stall force on surfaces coated with skeletal heavy meromyosin. In contrast, the G146V mutation had no effect on either gliding velocity or stall force on myosin V surfaces. Kinetic analyses of actin-myosin binding and ATPase activity indicated that the weaker affinity of actin filaments for myosin heads carrying ADP, as well as reduced actin-activated ATPase activity, are the cause of the diminished motility seen with skeletal myosin. Interestingly, the G146V mutation disrupted cooperative binding of myosin II heads to actin filaments. These data suggest that myosin-induced conformational changes in the actin filaments, presumably around the hinge region, are involved in mediating the motility of skeletal myosin but not myosin V and that the specific structural requirements for the actin subunits, and thus the mechanism of motility, differ among myosin classes.

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  • S witching of myosin-V motion between the lever-arm swing and Brownian search-and-catch Reviewed

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko H. Iwane, Lorenzo Marcucci, Toshio Yanagida

    NATURE COMMUNICATIONS   3   956   2012.7

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    DOI: 10.1038/ncomms1934

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  • Fluorescence microscopy for simultaneous observation of 3D orientation and movement and its application to quantum rod- tagged myosin V Reviewed

    Masashi Ohmachi, Yasunori Komori, Atsuko H. Iwane, Fumihiko Fujii, Takashi Jin, Toshio Yanagida

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   109 ( 14 )   5294 - 5298   2012.4

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    DOI: 10.1073/pnas.1118472109

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  • Simultaneous Observation of the Three-Dimensional Orientation and Position of a Single Fluorescent Probe

    Masashi Ohmachi, Yasunori Komori, Atsuko H. Iwane, Fumihiko Fujii, Takashi Jin, Toshio Yanagida

    BIOPHYSICAL JOURNAL   102 ( 3 )   721A - 721A   2012.1

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  • The Adjacent Binding State Enables Myosin VI Dual Function

    Keigo Ikezaki, Tomotaka Komori, Mitsuhiro Sugawa, Yoshiyuki Arai, So Nishikawa, Atsuko Hikikoshi Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   102 ( 3 )   569A - 569A   2012.1

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  • Single Molecule Measurement of the Myosin V Energy Transduction Process

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   102 ( 3 )   567A - 568A   2012.1

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  • 1PS049 Discovery of strain-sensor mechanism in motor protein and the quantification for the energy conversion(The 50th Annual Meeting of the Biophysical Society of Japan)

    Iwaki Mitsuhiro, Fujita Keisuke, Iwane Atsuko, Marcucci Lorenzo, Yanagida Toshio

    Seibutsu Butsuri   52   S82   2012

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    DOI: 10.2142/biophys.52.S82_5

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  • 1PT006 Single molecule analysis for the energy conversion of actomyosin in the presence of osmolyte(The 50th Annual Meeting of the Biophysical Society of Japan)

    Fujita Keisuke, Itoh Koji, Iwane Atsuko, Yanagida Toshio, Iwaki Mitsuhiro

    Seibutsu Butsuri   52   S84   2012

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    DOI: 10.2142/biophys.52.S84_3

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  • Multiple Actin Structures in Monomeric and Filamentous States

    Masatoshi Morimatsu, Yuichi Togashi, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   100 ( 3 )   33 - 33   2011.2

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  • Observation of Dynamical Conformational Changes of Skeletal Muscle Actin Filament

    Atsuko H. Iwane, Masatoshi Morimatsu, Toshio Yanagida

    BIOPHYSICAL JOURNAL   100 ( 3 )   299 - 299   2011.2

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  • Observation of Mechanical Process for Myosin-Ii by a Dark-Field Microscopy Combined with an Optical Trap

    Mitsuhiro Iwaki, Atsuko Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   100 ( 3 )   128 - 128   2011.2

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  • The Tail Domain of Myosin-VI Ensures the Directed Processive Movement

    Keigo Ikezaki, Tomotaka Komori, Mitsuhiro Sugawa, So Nishikawa, Atsuko Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   100 ( 3 )   119 - 119   2011.2

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  • Visualizing and Clarifying the Mechanical Properties of the Myosin V Brownian Search with Optical Tweezers

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   100 ( 3 )   117 - 117   2011.2

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  • Three Dimensional Orientation Measurements of Single Fluorescent Molecules by Newly Developed Polarized Fluorescence Microscopy

    Masashi Ohmachi, Yasunori Komori, Atsuko Iwane, Fumihiko Fujii, Toshio Yanagida

    BIOPHYSICAL JOURNAL   100 ( 3 )   116 - 116   2011.2

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  • 2M1636 Contribution of the lever arm to myosin VI's dual function as a transporter and an anchor(Molecular motor 3,The 48th Annual Meeting of the Biophysical Society of Japan)

    Ikezaki Keigo, Komori Tomotaka, Sugawa Mitsuhiro, Arai Yoshiyuki, Nishikawa So, Iwane Atsuko, Yanagida Toshio

    Seibutsu Butsuri   51   S99   2011

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    DOI: 10.2142/biophys.51.S99_1

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  • 3M0912 Single molecule measurement of the myosin V energy transduction process(Molecular motor4,The 49th Annual Meeting of the Biophysical Society of Japan)

    Fujita Keisuke, Iwaki Mitsuhiro, Iwane Atsuko, Yanagida Toshio

    Seibutsu Butsuri   51   S150   2011

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    DOI: 10.2142/biophys.51.S150_2

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  • 2SQ-02 Myosin mechanical-sensing for directional motion(2SQ Developing Mechanobiology:from fundamentals to medical applications,The 49th Annual Meeting of the Biophysical Society of Japan)

    Iwane Atsuko, Yanagida Toshio

    Seibutsu Butsuri   51   S25   2011

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    DOI: 10.2142/biophys.51.S25_5

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  • 3E1048 P07 1YE1030 High resolution structural analysis of the actin-myosin rigor complex by CryoEM(3E Muscle 1,The 49th Annual Meeting of the Biophysical Society of Japan)

    Fujii Takashi, Iwane Atsuko, Yanagida Toshio, Namba Keiichi

    Seibutsu Butsuri   51   S123   2011

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    DOI: 10.2142/biophys.51.S123_1

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  • Direct visualization of secondary structures of F-actin by electron cryomicroscopy Reviewed

    Takashi Fujii, Atsuko H. Iwane, Toshio Yanagida, Keiichi Namba

    NATURE   467 ( 7316 )   724 - U117   2010.10

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    DOI: 10.1038/nature09372

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  • Switch between large hand-over-hand and small inchworm-like steps in myosin VI. Reviewed International journal

    So Nishikawa, Ikuo Arimoto, Keigo Ikezaki, Mitsuhiro Sugawa, Hiroshi Ueno, Tomotaka Komori, Atsuko H Iwane, Toshio Yanagida

    Cell   142 ( 6 )   879 - 88   2010.9

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    Many biological motor molecules move within cells using stepsizes predictable from their structures. Myosin VI, however, has much larger and more broadly distributed stepsizes than those predicted from its short lever arms. We explain the discrepancy by monitoring Qdots and gold nanoparticles attached to the myosin-VI motor domains using high-sensitivity nanoimaging. The large stepsizes were attributed to an extended and relatively rigid lever arm; their variability to two stepsizes, one large (72 nm) and one small (44 nm). These results suggest that there exist two tilt angles during myosin-VI stepping, which correspond to the pre- and postpowerstroke states and regulate the leading head. The large steps are consistent with the previously reported hand-over-hand mechanism, while the small steps follow an inchworm-like mechanism and increase in frequency with ADP. Switching between these two mechanisms in a strain-sensitive, ADP-dependent manner allows myosin VI to fulfill its multiple cellular tasks including vesicle transport and membrane anchoring.

    DOI: 10.1016/j.cell.2010.08.033

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  • Mechanical Characterization of One-Headed Myosin-V Using Optical Tweezers Reviewed

    Tomonobu M. Watanabe, Atsuko H. Iwane, Hiroto Tanaka, Mitsuo Ikebe, Toshio Yanagida

    PLOS ONE   5 ( 8 )   1 - 11   2010.8

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    DOI: 10.1371/journal.pone.0012224

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  • Single Molecular Observation of Self-Regulated Kinesin Motility Reviewed

    Tomonobu M. Watanabe, Toshio Yanagida, Atsuko H. Iwane

    BIOCHEMISTRY   49 ( 22 )   4654 - 4661   2010.6

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    DOI: 10.1021/bi9021582

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  • Single-Molecule FRET Imaging for Enzymatic Reactions at High Ligand Concentrations Reviewed

    Mitsuhiro Sugawa, So Nishikawa, Atsuko Hikikoshi Iwane, Vasudevanpillai Biju, Toshio Yanagida

    SMALL   6 ( 3 )   346 - 350   2010.2

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    DOI: 10.1002/smll.200901827

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  • mNUDC is required for plus-end-directed transport of cytoplasmic dynein and dynactins by kinesin-1 Reviewed

    Masami Yamada, Shiori Toba, Takako Takitoh, Yuko Yoshida, Daisuke Mori, Takeshi Nakamura, Atsuko H. Iwane, Toshio Yanagida, Hiroshi Imai, Li-yuan Yu-Lee, Trina Schroer, Anthony Wynshaw-Boris, Shinji Hirotsune

    EMBO JOURNAL   29 ( 3 )   517 - 531   2010.2

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    DOI: 10.1038/emboj.2009.378

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  • Multiple Structural Forms of Actin in the Filamentous State

    Masatoshi Morimatsu, Yuichi Togashi, So Nishikawa, Mitsuhiro Sugawa, Atsuko H. Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   98 ( 3 )   154A - 154A   2010.1

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  • The Mechanical Properties of a Single Myosin V Motor Domain During Gait Motion

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   98 ( 3 )   562A - 563A   2010.1

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  • Single Molecule Visualization of Self-Regulated Kinesin Motility

    Tomonobu M. Watanabe, Toshio Yanagida, Atsuko H. Iwane

    BIOPHYSICAL JOURNAL   98 ( 3 )   369A - 370A   2010.1

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  • Shrec Measurement of Myosin-VI Stepping Motion

    Keigo Ikezaki, Mitsuhiro Sugawa, So Nishikawa, Tomotaka Komori, Atsuko Hikikoshi Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   98 ( 3 )   723A - 723A   2010.1

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  • G146V Mutant Actin is Defective in Conformational Changes, Accompanied by Impaired Motility with Skeletal Myosin

    Taro Q. P. Noguchi, Masatoshi Morimatsu, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida, Taro Q. P. Uyeda

    BIOPHYSICAL JOURNAL   98 ( 3 )   158A - 158A   2010.1

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  • Influence of Actin Mutant to Processive and Non-Processive Myosin Motility

    Tomotaka Komori, Hiroaki Takagi, Masatoshi Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   98 ( 3 )   146A - 146A   2010.1

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  • 1P201 Recombinant skeletal muscle actin for specific fluorescent labeling(Cell biology,The 48th Annual Meeting of the Biophysical Society of Japan)

    IWANE ATSUKO H., Morimatsu Masatoshi, Yanagida Toshio

    Seibutsu Butsuri   50 ( 2 )   S54 - S55   2010

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    DOI: 10.2142/biophys.50.S54_5

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  • 2P174 The regulation mechanism for myosin VI steps(The 48th Annual Meeting of the Biophysical Society of Japan)

    IKEZAKI KEIGO, SUGAWA MITSUHIRO, NISHIKAWA SO, KOMORI TOMOTAKA, IWANE ATSUKO, YANAGIDA TOSHIO

    Seibutsu Butsuri   50 ( 2 )   S113   2010

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    DOI: 10.2142/biophys.50.S113_1

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  • 3P145 Multiple actin structures in the filamentous state(Molecular motor,The 48th Annual Meeting of the Biophysical Society of Japan)

    Morimatsu Masatoshi, Togashi Yuichi, Iwane Atsuko, Yanagida Toshio

    Seibutsu Butsuri   50 ( 2 )   S170   2010

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    DOI: 10.2142/biophys.50.S170_4

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  • 1P149 1D1420 Direct visualization of secondary structures of F-actin by electron cryomicroscopy(Muscle,Oral Presentations,The 48th Annual Meeting of the Biophysical Society of Japan)

    Fujii Takashi, Iwane Atsuko, Yanagida Toshio, Namba Keiichi

    Seibutsu Butsuri   50 ( 2 )   S45   2010

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    DOI: 10.2142/biophys.50.S45_5

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  • 2P183 Visualizing and clarifying the mechanical properties of the Myosin V Brownian search with optical tweezers(The 48th Annual Meeting of the Biophysical Society of Japan)

    Fujita Keisuke, Iwaki Mitsuhiro, Iwane Atsuko, Yanagida Toshio

    Seibutsu Butsuri   50 ( 2 )   S114   2010

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    DOI: 10.2142/biophys.50.S114_5

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  • 2P185 Observation of mechanical (elementary) process for myosin II by dark-field microscopy combined with an optical trap(The 48th Annual Meeting of the Biophysical Society of Japan)

    Iwaki Mitsuhiro, Iwane Atsuko, Yanagida Toshio

    Seibutsu Butsuri   50 ( 2 )   S115   2010

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    DOI: 10.2142/biophys.50.S115_1

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  • Recombinant alpha-actin for specific fluorescent labeling Reviewed

    Atsuko H. Iwane, Masatoshi Morimatsu, Toshio Yanagida

    PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES   85 ( 10 )   491 - 499   2009.12

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    DOI: 10.2183/pjab.85.491

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  • Brownian search-and-catch mechanism for myosin-VI steps Reviewed

    Mitsuhiro Iwaki, Atsuko H. Iwane, Tetsuya Shimokawa, Roger Cooke, Toshio Yanagida

    NATURE CHEMICAL BIOLOGY   5 ( 6 )   403 - 405   2009.6

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    DOI: 10.1038/nchembio.171

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  • High Speed Imaging for Myosin VI.

    Arimoto I, Nishikawa S, Sugawa M, Iwane AH, Yanagida T

    Biophysical Journal   96 ( 3 )   139a   2009.2

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    DOI: 10.1016/j.bpj.2008.12.3868

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  • Simultaneous Measurement of Nucleotide Occupancy and Mechanical Displacement in Myosin-V, a Processive Molecular Motor Reviewed

    Tomotaka Komori, So Nishikawa, Takayuki Ariga, Atsuko Hikikoshi Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   96 ( 1 )   L4 - L6   2009.1

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    DOI: 10.1016/j.bpj.2008.09.031

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  • 2P-124 Development of new microscopy for determining three dimensional orientations of single molecules(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Ohmachi Masashi, Komori Yasunori, Iwane Atsuko H., Yanagida Toshio

    Seibutsu Butsuri   49   S125 - S126   2009

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    DOI: 10.2142/biophys.49.S125_6

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  • 3P-124 Visualization of self-regulated kinesin motility.(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Iwane Atsuko, Watanabe Tomonobu, Yanagida Toshio

    Seibutsu Butsuri   49   S172   2009

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    DOI: 10.2142/biophys.49.S172_1

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  • 3P-125 G146V mutation in actin inhibits force generation during interaction with skeletal myosin.(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Noguchi Taro, Morimatsu Masatoshi, Ito Kohji, Iwane Atsuko, Yanagida Toshio, Uyeda taro

    Seibutsu Butsuri   49   S172   2009

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    DOI: 10.2142/biophys.49.S172_2

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  • 3P-119 3D tracking of motor proteins(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Martin Thomas, Nishikawa So, Sugawa Mitsuhiro, Iwane Atsuko, Yanagida Toshio

    Seibutsu Butsuri   49   S171   2009

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    DOI: 10.2142/biophys.49.S171_3

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  • 3P-118 Two Distinct Forms of Actin in Monomeric and Filamentous states(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Morimatsu Masatoshi, Togashi Yuichi, Nishikawa So, Sugawa Mitsuhiro, Iwane Atsuko, Yanagida Toshio

    Seibutsu Butsuri   49   S171   2009

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    DOI: 10.2142/biophys.49.S171_2

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  • 3P-120 Quantitative analysis of strain-sensor for myosin-VI(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Iwaki Mitsuhiro, Iwane Atsuko, Shimokawa Tetsuya, Yanagida Toshio

    Seibutsu Butsuri   49   S171   2009

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    DOI: 10.2142/biophys.49.S171_4

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  • 3P-122 The mechanical properties of a single myosin V motor domain during gait motion(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Fujita Keisuke, Iwaki Mitsuhiro, Iwane Atsuko, Morimatsu Masatoshi, Yanagida Toshio

    Seibutsu Butsuri   49   S171   2009

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    DOI: 10.2142/biophys.49.S171_6

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  • 2P-131 SHREC measurement of myosin-VI stepping motion(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Ikezaki Keigo, Sugawa Mitsuhiro, Nishikawa So, Iwane Atsuko, Yanagida Toshio

    Seibutsu Butsuri   49   S127   2009

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    DOI: 10.2142/biophys.49.S127_1

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  • Fluctuation analysis of mechanochemical coupling depending on the type of biomolecular motors Reviewed

    Masatoshi Nishikawa, Hiroaki Takagi, Tatsuo Shibata, Atsuko H. Iwane, Toshio Yanagida

    PHYSICAL REVIEW LETTERS   101 ( 12 )   128103 - 128106   2008.9

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    DOI: 10.1103/PhysRevLett.101.128103

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  • Measurement system for simultaneous observation of myosin V chemical and mechanical events Reviewed

    Tomotaka Komori, So Nishikawa, Takayuki Ariga, Atsuko Hikikoshi Iwane, Toshio Yanagida

    BIOSYSTEMS   93 ( 1-2 )   48 - 57   2008.7

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    DOI: 10.1016/j.biosystems.2008.04.004

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  • Biased Brownian motion mechanism for processivity and directionality of single-headed myosin-VI Reviewed

    Mitsuhiro Iwaki, Atsuko Hikikoshi Iwane, Mitsuo Ikebe, Toshio Yanagida

    BIOSYSTEMS   93 ( 1-2 )   39 - 47   2008.7

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    DOI: 10.1016/j.biosystems.2008.03.013

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  • Simultaneous Observation of Individual Mechanochemical Events of Myosin V during Processive 36 nm Steps.

    Sugawa M, Nishikawa S, Iwane AH, Yanagida T

    Biophysical Journal   94   2008

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  • Relationship between Myosin Va ATPase activity and motility.

    Iwane AH, Mimuro T, Nishikawa M, Komori T, Yanagida T

    Biophysical Journal   94   2008

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  • Myosin-V makes two 90゜Brownian rotation per 36nm step.

    Komori Y, Iwane AH, Yanagida T

    Biophysical Journal   94   2008

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  • Spontaneous conformational fluctuations in cell signaling proteins of Ras Reviewed

    Yoshiyuki Arai, Atsuko H. Iwane, Yoshiharu Ishii, Toshio Yanagida

    PROGRESS OF THEORETICAL PHYSICS SUPPLEMENT   173 ( 173 )   229 - 234   2008

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  • Direct observation of chemo-mechanical coupling in single headed myosin V”

    Komori T, Nishikawa S, Ariga T, Iwane AH, Yanagida T

    Biophysical Journal   94   2008

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  • Mechanochemical Coupling In Actomyosin Motility With Fluctuation Analysis.

    Nishikawa M, Takagi Iwane AH, Yanagida T

    Biophysical Journal   94   2008

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  • Strain-dependent search and capture mechanism for directional motion of myosin-VI.

    Iwaki M, Iwane AH, Yanagida T

    Biophysical Journal   94   2008

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  • 1P-147 The role of the Magnesium ion in the ATPase activity and motility of Myosin Va(The 46th Annual Meeting of the Biophysical Society of Japan)

    Iwane Atsuko H., Mimuro Takako, Nishikawa Masatoshi, Komori Tomotaka, Togashi Yuichi, Ogawa Ruriko, Yanagida Toshio

    Seibutsu Butsuri   48   S44   2008

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    DOI: 10.2142/biophys.48.S44_3

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  • 1P-132 Strain-dependent search and capture mechanism for directionat motion of myosin-VI(The 46th Annual Meeting of the Biophysical Society of Japan)

    Iwaki Mitsuhiro, Iwane Atsuko H., Yanagada Toshio

    Seibutsu Butsuri   48   S41   2008

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    DOI: 10.2142/biophys.48.S41_6

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  • 1P-162 High-speed scanning experiment to apply a strain to myosin II before the transition to the strong binding state(The 46th Annual Meeting of the Biophysical Society of Japan)

    Ran Tomohisa, Iwaki Mitsuhiro, Iwane Atsuko H., Yanagida Toshio

    Seibutsu Butsuri   48   S46   2008

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    DOI: 10.2142/biophys.48.S46_4

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  • 2P-198 Single myosin VI tracking with improved time resolution(The 46th Annual Meeting of the Biophysical Society of Japan)

    Arimoto Ikuo, Nishikawa So, Sugawa Matsuhiro, Iwane Atsuko H., Yanagida Toshio

    Seibutsu Butsuri   48   S105   2008

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    DOI: 10.2142/biophys.48.S105_5

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  • 2P-172 Single myosin V tracking with improved time resolution(The 46th Annual Meeting of the Biophysical Society of Japan)

    Nishikawa So, Sugawa Mitsuhiro, Ueno Hiroshi, Iwane Atsuko H., Yanagida Toshio

    Seibutsu Butsuri   48   S101   2008

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    DOI: 10.2142/biophys.48.S101_6

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  • 1P-139 Simultaneous observation of individual mechanochermcal events of myosin V during processive 36nm steps(The 46th Annual Meeting of the Biophysical Society of Japan)

    Sugawa Mitsuhiro, Nishikawa So, Iwane Atsuko H., Yanagida Toshio

    Seibutsu Butsuri   48   S42 - S43   2008

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    DOI: 10.2142/biophys.48.S42_6

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  • 2P-167 Site-directed single molecule FRET measurement to clarify the intra molecular dynamics of actin(The 46th Annual Meeting of the Biophysical Society of Japan)

    Morimatsu Masatoshi, Nishikawa So, Sugawa Mitsuhiro, Iwane Atsuko H, Yanagida Toshio

    Seibutsu Butsuri   48   S101   2008

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    DOI: 10.2142/biophys.48.S101_1

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  • 2P-150 Direct observation of a single myosin II molecule dynamics within a myofibril(The 46th Annual Meeting of the Biophysical Society of Japan)

    Komori Yasunori, Iwane Atsuko H., Yanagida Toshio

    Seibutsu Butsuri   48   S98   2008

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    DOI: 10.2142/biophys.48.S98_3

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  • 1P-149 Simultaneous observation of nucleotide occupancy and mechanical displacement in a processive motor myosin-V(The 46th Annual Meeting of the Biophysical Society of Japan)

    Komori Tomotaka, Nishikawa So, Ariga Takayuki, Iwane Atsuko H., Yanagida Toshio

    Seibutsu Butsuri   48   S44   2008

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    DOI: 10.2142/biophys.48.S44_5

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  • Myosin-V makes two brownian 90 degrees rotations per 36-nm step Reviewed

    Yasunori Komori, Atsuko H. Iwane, Toshio Yanagida

    NATURE STRUCTURAL & MOLECULAR BIOLOGY   14 ( 10 )   968 - 973   2007.10

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    DOI: 10.1038/nsmb1298

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  • Myosin-V makes two brownian 90° rotations per 36-nm step Reviewed

    Yasunori Komori, Atsuko H. Iwane, Toshio Yanagida

    Nature Structural and Molecular Biology   14 ( 10 )   968 - 973   2007.10

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    DOI: 10.1038/nsmb1298

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  • Role of multiple bonds between the single cell adhesion molecules, nectin and cadherin, revealed by high sensitive force measurements Reviewed

    Yoshikazu Tsukasaki, Kazuo Kitamura, Kazuya Shimizu, Atsuko H. Iwane, Yoshimi Takai, Toshio Yanagida

    JOURNAL OF MOLECULAR BIOLOGY   367 ( 4 )   996 - 1006   2007.4

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    DOI: 10.1016/j.jmb.2006.12.022

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  • Single molecule FRET for the study on structural dynamics of biomolecules Reviewed

    Mitsuhiro Sugawa, Yoshiyuki Arai, Atsuko Hikikoshi Iwane, Yoshiharu Ishii, Toshio Yanagida

    BIOSYSTEMS   88 ( 3 )   243 - 250   2007.4

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    DOI: 10.1016/j.biosystems.2006.09.041

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  • The diffusive search mechanism of processive myosin class-V motor involves directional steps along actin subunits Reviewed

    Takuya Okada, Hiroto Tanaka, Atsuko Hikikoshi Iwane, Kazuo Kitamura, Mitsuo Ikebe, Toshio Yanagida

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   354 ( 2 )   379 - 384   2007.3

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    DOI: 10.1016/j.bbrc.2006.12.200

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  • 2P126 Simultaneous observation of individual mechanochemical events of myosin V during processive 36 nm steps(Molecular motors,Poster Presentations)

    Sugawa Mitsuhiro, Nishikawa So, Iwane Atsuko, Yanagida Toshio

    Seibutsu Butsuri   47   S144   2007

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    DOI: 10.2142/biophys.47.S144_3

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  • 2P136 Strain-dependent strong binding of myosin to actin revealed by high-speed scanning experiment(Molecular motors,Oral Presentations)

    Iwaki Mitsuhiro, Iwane Atsuko H., Yanagida Toshio

    Seibutsu Butsuri   47   S147   2007

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    DOI: 10.2142/biophys.47.S147_1

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  • 2P124 Direct observation of mechanochemical coupling in single-headed myosin V(Molecular motors,Oral Presentations)

    Komori Tomotaka, Nishikawa So, Ariga Takayuki, Iwane Atsuko H., Yanagida Toshio

    Seibutsu Butsuri   47   S144   2007

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    DOI: 10.2142/biophys.47.S144_1

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  • 2P141 Fluctuation analysis of actomyosin sliding movement and mechanochemical coupling(Molecular motors,Poster Presentations)

    Nishikawa Masatoshi, Takagi Hiroaki, Iwane Atsuko H., Yanagida Toshio

    Seibutsu Butsuri   47   S148   2007

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    DOI: 10.2142/biophys.47.S148_2

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  • 2P123 Relationship between actin-activated ATPase activity and motility of Myosin Va.(Molecular motors,Poster Presentations)

    Iwane Atsuko H., Nishikawa Masatoshi, Komori Tomotaka, Mimuro Takako, Ariga Takayuki, Yanagida Toshio

    Seibutsu Butsuri   47   S143   2007

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    DOI: 10.2142/biophys.47.S143_4

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  • 2P127 Two Brownian 90° Rotations of Myosin-V during 36 nm-Step(Molecular motors,Poster Presentations)

    Komori Yasunori, Iwane Atsuko H., Yanagida Toshio

    Seibutsu Butsuri   47   S144   2007

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    DOI: 10.2142/biophys.47.S144_4

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  • Dynamic polymorphism of Ras observed by single molecule FRET is the basis for molecular recognition Reviewed

    Y Arai, AH Iwane, T Wazawa, H Yokota, Y Ishii, T Kataoka, T Yanagida

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   343 ( 3 )   809 - 815   2006.5

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    DOI: 10.1016/j.bbrc.2006.03.031

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  • Cargo-binding makes a wild-type single-headed myosin-VI move processively Reviewed

    M Iwaki, H Tanaka, AH Iwane, E Katayama, M Ikebe, T Yanagida

    BIOPHYSICAL JOURNAL   90 ( 10 )   3643 - 3652   2006.5

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    DOI: 10.1529/biophysj.105.075721

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  • A unique mechanism for the processive movement of single-headed myosin-IX Reviewed

    M Nishikawa, S Nishikawa, A Inoue, AH Iwane, T Yanagida, M Ikebe

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   343 ( 4 )   1159 - 1164   2006.5

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    DOI: 10.1016/j.bbrc.2006.03.057

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  • Grafting of poly(ethylene glycol) onto poly(acrylic acid)-coated glass for a protein-resistant surface Reviewed

    T Wazawa, Y Ishizuka-Katsura, S Nishikawa, AH Iwane, S Aoyama

    ANALYTICAL CHEMISTRY   78 ( 8 )   2549 - 2556   2006.4

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    DOI: 10.1021/ac052102j

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  • 1P299 The micro needle study of the relationship between myosin and a single actin filament(9. Molecular motor (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Morimatsu Masatoshi, Nishikawa So, Tsukasaki Yoshikazu, Okada Takuya, Iwane Atsuko H, Yanagida Toshio

    Seibutsu Butsuri   46 ( 2 )   S221   2006

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    DOI: 10.2142/biophys.46.S221_3

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  • 2P515 Single molecule FRET imaging of enzymatic reactions at high concentrations of fluorescently labeled ligands(52. Bio-imaging,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Sugawa Mitsuhiro, Nishikawa So, Iwane Atsuko H., Mimuro Takako, Yanagida Toshio

    Seibutsu Butsuri   46 ( 2 )   S424   2006

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    DOI: 10.2142/biophys.46.S424_3

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  • 2P013 Multiple conformational changes of Ras observed by single molecule FRET(29. Protein structure and dynamics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Arai Yoshiyuki, Iwane Atsuko H, Wazawa Tetsuichi, Yokota Hiroaki, Ishii Yoshiharu, Kataoka Tohru, Yanagida Toshio

    Seibutsu Butsuri   46 ( 2 )   S299   2006

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    DOI: 10.2142/biophys.46.S299_1

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  • 1P541 Multiple domain interactions between the single cell adhesion molecule, nectin and cadherin, revealed by high sensitive force measurements(26. Single molecule biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Tsukasaki Yoshikazu, Kitamura Kazuo, Shimizu Kazuya, Iwane Atsuko_H., Takai Yoshimi, Yanagida Toshio

    Seibutsu Butsuri   46 ( 2 )   S282   2006

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    DOI: 10.2142/biophys.46.S282_1

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  • 2P196 Coiled-coil domain of myosin V(37. Molecular motor (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Ariga Takayuki, Komori Tomotaka, Iwane Atsuko_H., Yanagida Toshio

    Seibutsu Butsuri   46 ( 2 )   S344   2006

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    DOI: 10.2142/biophys.46.S344_4

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  • 1P531 Self-regulated kinesin motility(26. Single molecule biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Watanabe Tomonobu M., Yanagida Toshio, Iwane Atsuko H.

    Seibutsu Butsuri   46 ( 2 )   S279   2006

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    DOI: 10.2142/biophys.46.S279_3

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  • The neck domain of myosin II primarily regulates the actomyosin kinetics, not the stepsize Reviewed

    AH Iwane, H Tanaka, S Morimoto, A Ishijima, T Yanagida

    JOURNAL OF MOLECULAR BIOLOGY   353 ( 2 )   213 - 221   2005.10

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    DOI: 10.1016/j.jmb.2005.08.013

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  • Cargo binding makes a single-headed myosin-VI move processively

    M Iwaki, H Tanaka, AH Iwane, E Katayama, M Ikebe, T Yanagida

    BIOPHYSICAL JOURNAL   88 ( 1 )   647A - 647A   2005.1

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  • 2P196 Processive movement of Myosin V without swing of lever arm domain

    Komori Y., Iwane A.H., Ikebe M., Yanagida T.

    Seibutsu Butsuri   45   S168   2005

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    DOI: 10.2142/biophys.45.S168_4

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  • 2P194 Single molecule observation of ATP hydrolysis of Myosin V

    Komori T., Nishikawa S., Ariga T., Iwane A.H., Yamakawa H., Ohara O., Yanagida T.

    Seibutsu Butsuri   45   S168   2005

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    DOI: 10.2142/biophys.45.S168_2

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  • 2P320 Development of a novel microscope for sirmultaneous observation of mechanochemical coupling

    Ariga T., Komori T., Nishikawa S., Iwane A., Yanagida T.

    Seibutsu Butsuri   45   S199   2005

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    DOI: 10.2142/biophys.45.S199_4

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  • 1P049 Single molecule FRET observation of conformation in mutant Ras incorporated fluorescence dye into the its SwitchII region

    ARAI Y., Wazawa T., Iwane A., Yokota H., Ishii Y., Kataoka T., Yanagida T.

    Seibutsu Butsuri   45   S44   2005

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    DOI: 10.2142/biophys.45.S44_1

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  • 1P113 Single-molecule imaging of cyanobacterial circadian clock proteins

    Wada C., Arai Y., Hayashi F., Iwase R., Uzumaki T., Iwane A.H., Yanagida T., Ishiura M.

    Seibutsu Butsuri   45   S60   2005

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    DOI: 10.2142/biophys.45.S60_1

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  • Mechanism of muscle contraction based on stochastic properties of single actomyosin motors observed in vitro. Reviewed

    Kitamura Kazuo, Tokunaga Makio, Esaki Seiji, Hikikoshi Iwane Atsuko, Yanagida Toshio

    BIOPHYSICS   1:1-19   1 - 19   2005

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    We have previously measured the process of displacement generation by a single head of muscle myosin (S1) using scanning probe nanometry. Given that the myosin head was rigidly attached to a fairly large scanning probe, it was assumed to stably interact with an underlying actin filament without diffusing away as would be the case in muscle. The myosin head has been shown to step back and forth stochastically along an actin filament with actin monomer repeats of 5.5 nm and to produce a net movement in the forward direction. The myosin head underwent 5 forward steps to produce a maximum displacement of 30 nm per ATP at low load (<1 pN). Here, we measured the steps over a wide range of forces up to 4 pN. The size of the steps (~5.5 nm) did not change as the load increased whereas the number of steps per displacement and the stepping rate both decreased. The rate of the 5.5-nm steps at various force levels produced a force-velocity curve of individual actomyosin motors. The force-velocity curve from the individual myosin heads was comparable to that reported in muscle, suggesting that the fundamental mechanical properties in muscle are basically due to the intrinsic stochastic nature of individual actomyosin motors. In order to explain multiple stochastic steps, we propose a model arguing that the thermally-driven step of a myosin head is biased in the forward direction by a potential slope along the actin helical pitch resulting from steric compatibility between the binding sites of actin and a myosin head. Furthermore, computer simulations show that multiple cooperating heads undergoing stochastic steps generate a long (>60 nm) sliding distance per ATP between actin and myosin filaments, i.e., the movement is loosely coupled to the ATPase cycle as observed in muscle.<br>

    DOI: 10.2142/biophysics.1.1

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  • 1P180 Biased diffusional motion of cargo bound single-headed myosin-VI observed in high viscous solution

    Iwaki M., Ikebe M., Yanagida T.

    Seibutsu Butsuri   45   S76   2005

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    DOI: 10.2142/biophys.45.S76_4

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  • 2P117 Direct Observation of Cell adhesion molecule Nanoreaction-field Using Flexible Intermolecular Force Microscopy

    Tsukasaki Y., Kitamura K., Shimizu K., Iwane A.H., Takai Y., Yanagida T.

    Seibutsu Butsuri   45   S149   2005

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    DOI: 10.2142/biophys.45.S149_1

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  • 2P116 Quantitative single molecule FRET measurement for structural dynamics study

    Sugawa M., Arai Y., Iwane A.H., Ishii Y., Yanagida T.

    Seibutsu Butsuri   45   S148   2005

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    DOI: 10.2142/biophys.45.S148_4

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  • 3P176 A single head myosin V moves along an actin filament with 5.6 nm steps

    Okada T.okada, Kitamura K., Tanaka H., Iwane A.H., Ikebe M., Yanagida T.

    Seibutsu Butsuri   45   S247   2005

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    DOI: 10.2142/biophys.45.S247_4

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  • 3P177 Easy system to detect the step by using glass needle

    Morimatsu M., Nishikawa S., Tsukasaki Y., Okada T., Iwane H., Yanagida T.

    Seibutsu Butsuri   45   S248   2005

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    DOI: 10.2142/biophys.45.S248_1

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  • Dynamic polymorphism of a cellular signal protein Ras studied by single molecule FREt: How does Ras interact with many effectors to regulate signaling pathways?

    Y Arai, M Sugawa, AH Iwane, T Yanagida

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   228   U206 - U206   2004.8

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  • Single-molecule imaging of the dynamic interactions between macromolecules Reviewed

    H Yokota, K Kaseda, H Matsuura, Y Arai, A Iwane, Y Ishii, T Kodama, T Yanagida

    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY   4 ( 6 )   616 - 621   2004.7

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    DOI: 10.1166/jnn.2004.009

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  • Unconstrained steps of myosin VI appear longest among known molecular motors Reviewed

    MY Ali, K Homma, AH Iwane, K Adachi, H Itoh, K Kinosita, T Yanagida, M Ikebe

    BIOPHYSICAL JOURNAL   86 ( 6 )   3804 - 3810   2004.6

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    DOI: 10.1529/biophysj.103.037416

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  • A one-headed class V myosin molecule develops multiple large (approximate to 32-nm) steps successively Reviewed

    TM Watanabe, H Tanaka, AH Iwane, S Maki-Yonekura, K Homma, A Inoue, R Ikebe, T Yanagida, M Ikebe

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   101 ( 26 )   9630 - 9635   2004.6

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    DOI: 10.1073/pnas.040291401

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  • Single molecule Interactions between cell adhesion molecules, Nectin and Cadherin, Probed by Intermolecular Force Microscopy

    Yoshikazu Tsukasaki, Kazuo Kitamura, Kazuya Shimizu, Atsuko H. Iwane, Yoshimi Takai, Toshio Yanagida

    CELL STRUCTURE AND FUNCTION   29   41 - 41   2004.5

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  • Asymmetric binding of myosin V and VI to an actin filament

    Y Komori, H Tanaka, AH Iwane, Y Ishii, M Ikebe, T Yanagida

    BIOPHYSICAL JOURNAL   86 ( 1 )   54A - 54A   2004.1

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  • 1P031 Development of a quantitative measurement system for single molecule FRET

    Sugawa M., Arai Y., Iwane A.H., Yanagida T.

    Seibutsu Butsuri   44   S37   2004

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    DOI: 10.2142/biophys.44.S37_3

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  • 3P151 The method of force measurment, after checking single molecules

    Nishikawa S., Nishikawa M., Zulliger Martin, Iwane A.H., Yanagida T.

    Seibutsu Butsuri   44   S227   2004

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    DOI: 10.2142/biophys.44.S227_3

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  • 1P030 Novel stable interactions between cell adhesion molecules detected using intermolecular force microscopy at sub-piconewton resolution

    Tsukasaki Y., kitamura K., Shimizu K., Iwane A. H., Takai Y., Yanagida T.

    Seibutsu Butsuri   44   S37   2004

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    DOI: 10.2142/biophys.44.S37_2

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  • Single- and multiple-molecule dynamics of the signaling from H-Ras to cRaf-1 visualized on the plasma membrane of living cells Reviewed

    K Hibino, TM Watanabe, J Kozuka, AH Iwane, T Okada, T Kataoka, T Yanagida, Y Sako

    CHEMPHYSCHEM   4 ( 7 )   748 - 753   2003.7

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    DOI: 10.1002/cphc.200300731

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  • Single molecule nanometry of single-headed myosin-V by optical tweezer

    TM Watanabe, H Tanaka, AH Iwane, K Homma, R Ikebe, M Ikebe, T Yanagida

    BIOPHYSICAL JOURNAL   84 ( 2 )   117A - 117A   2003.2

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  • Load dependence of step size and kinetics of single myosin VI molecules

    M Iwaki, H Tanaka, AH Iwane, K Homma, R Ikebe, T Yanagida, M Ikebe

    BIOPHYSICAL JOURNAL   84 ( 2 )   117A - 117A   2003.2

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  • Simultaneous observation of rotatory and stepping motions of single myosin VI molecules

    S Nishikawa, K Kitamura, H Tanaka, AH Iwane, K Homma, R Ikebe, M Ikebe, T Yanagida

    BIOPHYSICAL JOURNAL   84 ( 2 )   115A - 115A   2003.2

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  • Multiple forms of activated Ras observed directly by single molecule FRET

    Y Arai, AH Iwane, T Wazawa, H Yokota, Y Ishii, T Kataoka, T Yanagida

    BIOPHYSICAL JOURNAL   84 ( 2 )   37A - 37A   2003.2

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  • Introduction : Nobel Techniques for Live Cell Imaging

    Miyauchi T., Iwane A.H.

    Seibutsu Butsuri   43   S16   2003

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    DOI: 10.2142/biophys.43.S16_3

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  • Mechanical property of single myosin VI molecules which can not be explained by lever-arm hypothesis

    Iwaki M., Tanaka H., Iwane A., Ikebe M., Yanagida T.

    Seibutsu Butsuri   43   S144   2003

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    DOI: 10.2142/biophys.43.S144_4

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  • Substeps of myosin V

    Okada T., Kitamura K., Iwane A., Tanaka H., Ikebe M., Yanagida T.

    Seibutsu Butsuri   43   S142   2003

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    DOI: 10.2142/biophys.43.S142_3

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  • The two-headed structure of myosin-V stabilizes its processive movement.

    Watanabe T.M., Tanaka H., Iwane H., Maki-Yonekura S., Homma K., Ikebe R., Ikebe Mitsuo, Yanagida T.

    Seibutsu Butsuri   43   S143   2003

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    DOI: 10.2142/biophys.43.S143_1

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  • Multiple conformations of activated Ras observed directly by single molecule FRET

    Arai Y., Wazawa T., Yokota H., Iwane A.H., Ishii Y., Kataoka Kataoka, Yanagida T

    Seibutsu Butsuri   43   S49   2003

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    DOI: 10.2142/biophys.43.S49_3

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  • Motor function of unconventional myosin

    M Ikebe, A Inoue, S Nishikawa, K Homma, H Tanaka, AH Iwane, E Katayama, R Ikebe, T Yanagida

    MOLECULAR AND CELLULAR ASPECTS OF MUSCLE CONTRACTION   538   143 - 157   2003

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    DOI: 10.1007/978-1-4419-9029-7_13

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  • Cell Adhesion Molecules Interaction Probed Using Intermolecular Force Microscopy

    Tsukasaki Y., Kitamura K., shimizu K., Iwane A.H., Takai Y., Yanagida T.

    Seibutsu Butsuri   43   S71   2003

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    DOI: 10.2142/biophys.43.S71_4

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  • The binding potential of actmyosin revealed by single molecule imaging

    Komori Y., Tanaka H., Iwane A.H., Ikebe M., Yanagida T.

    Seibutsu Butsuri   43   S141   2003

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    DOI: 10.2142/biophys.43.S141_2

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  • 分子機械の作動原理--あいまいさと生体機能 (特集 エネルギー変換装置ATPaseの作動原理)

    岩根 敦子, 小森 靖則, 岩城 光宏

    蛋白質核酸酵素   47 ( 9 )   1157 - 1164   2002.7

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  • [Approach to engineering principle of adaptive biological systems by uncovering unique operation of bio-molecular machines]. Reviewed

    Iwane AH, Komori Y, Iwaki M, Yanagida T

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   47 ( 9 )   1157 - 1164   2002.7

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  • Actin plays an important role in the processive 36nm steps of class V and VI myosin

    E Katayama, TQP Uyeda, AH Iwane, M Ikebe, T Yanagida

    BIOPHYSICAL JOURNAL   82 ( 1 )   15A - 16A   2002.1

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  • Ras takes multiple conformations to adapt to its multiple effectors

    Y Arai, H Yokota, AH Iwane, T Wazawa, T Kataoka, Y Ishii, T Yanagida

    BIOPHYSICAL JOURNAL   82 ( 1 )   452A - 452A   2002.1

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  • Myosin VI moves processively along actin filamments backwards with large 38nm steps Reviewed

    S Nishikawa, K Homma, Y Komori, M Iwaki, AH Iwane, J Saito, R Ikebe, E Katayama, T Yanagida, M Ikebe

    BIOPHYSICAL JOURNAL   82 ( 1 )   408A - 408A   2002.1

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  • Class VI myosin moves processively along actin filaments backward with large steps Reviewed

    S Nishikawa, K Homma, Y Komori, M Iwaki, T Wazawa, AH Iwane, J Saito, R Ikebe, E Katayama, T Yanagida, M Ikebe

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   290 ( 1 )   311 - 317   2002.1

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    DOI: 10.1006/bbrc.2001.6142

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  • The motor domain determines the large step of myosin-V

    H Tanaka, K Homma, AH Iwane, E Katayama, R Ikebe, J Saito, T Yanagida, M Ikebe

    BIOPHYSICAL JOURNAL   82 ( 1 )   374A - 374A   2002.1

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  • Subcellular dynamics of the localization of Ras and Raf1 visualized in living cells stimulated with EGF

    K Hibino, Y Sako, AH Iwane, T Kataoka, T Yanagida

    BIOPHYSICAL JOURNAL   82 ( 1 )   222A - 222A   2002.1

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  • Single molecule imaging of the kinesin's self-regulated motility

    T Watanabe, AH Iwane, T Yanagida

    BIOPHYSICAL JOURNAL   82 ( 1 )   64A - 64A   2002.1

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  • 1E1045 Simultaneous Observation of Processive Movements and Rotatory Motions of a Single Myosin VI Molecule

    Nishikawa S., Kitamura K., Tanaka H., Iwane A. H., Ikebe M., Yanagida T.

    Seibutsu Butsuri   42 ( 2 )   S30   2002

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    DOI: 10.2142/biophys.42.S30_3

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  • Myosin VI moves processively along actin filaments backwards with large 38nm steps.

    Nishikawa S, Homma K, Komori Y, Iwaki M, Iwane AH, Saito J, Ikebe R, Katayama E, Yanagida T, Ikebe M

    Biophysical Journal   82   408   2002

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  • 3E1400 Orientation dependent processive movement of myosin-V

    Tanaka H., Watanabe T., Homma K., Iwane A., Ikebe R., Ikebe M., Yanagida T.

    Seibutsu Butsuri   42 ( 2 )   S160   2002

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    DOI: 10.2142/biophys.42.S160_3

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  • 3E1415 Conformational changes of myosin V observed by singlemolecule fluorometry

    Wazawa T., Ishii Y., Sugihara K., Iwane A., Homma K., Ikebe M., Yanagida T.

    Seibutsu Butsuri   42 ( 2 )   S160   2002

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    DOI: 10.2142/biophys.42.S160_4

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  • 3E1345 A single one-headed myosin-V moves processively with 32 nm steps

    Watanabe T., Tanaka H., Iwane A.H., Maki S., Homma K., Ikebe R., Ikebe M., Yanagida T.

    Seibutsu Butsuri   42 ( 2 )   S160   2002

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    DOI: 10.2142/biophys.42.S160_2

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  • 3K1345 Imaging of conformational changes in single Ras molecule by FRET

    Arai Y., Wazawa T., Yokota H., Iwane H., Ishii Y., Kataoka T., Yanagida T.

    Seibutsu Butsuri   42 ( 2 )   S182   2002

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    DOI: 10.2142/biophys.42.S182_2

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  • 3E1430 Conformational changes of actin interacting with myosin V monitored by single molecule FRET

    Kozuka J., Yokota H., Iwane A., Ishii Y., Ikebe M., Yanagida T.

    Seibutsu Butsuri   42 ( 2 )   S161   2002

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    DOI: 10.2142/biophys.42.S161_1

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  • 3E1445 Detection of Snbsteps of Myosin V using a scanning probe

    Okada T., Kitamura K., Iwane A., Ikebe M., Yanagida T.

    Seibutsu Butsuri   42 ( 2 )   S161   2002

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    DOI: 10.2142/biophys.42.S161_2

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  • 3E1045 single molecule anlysis of Myosin VI measured by optical tweezers

    Iwaki M., Tanaka H., Iwane A.H., Nishikawa S., Ikebe M., Yanagida T.

    Seibutsu Butsuri   42 ( 2 )   S158   2002

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    DOI: 10.2142/biophys.42.S158_4

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  • Human myosin IX is a single headed processive motor

    A Inoue, J Saito, S Nishikawa, A Iwane, T Yanagida, M Ikebe

    MOLECULAR BIOLOGY OF THE CELL   12   428A - 428A   2001.11

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  • Motility of single one-headed kinesin molecules along microtubules Reviewed

    Y Inoue, AH Iwane, T Miyai, E Muto, T Yanagida

    BIOPHYSICAL JOURNAL   81 ( 5 )   2838 - 2850   2001.11

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  • The regulation of the Kinesin's tail domain by single molecule imaging

    WATANABE Tomonobu, IWANE Atsuko H., YANAGIDA Toshio

    Seibutsu Butsuri   41   S193   2001

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    DOI: 10.2142/biophys.41.S193_3

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  • Stochastic properties of the stepping motion produced by a single myosin head

    Kitamura K., Tokunaga M., Iwane A.H., Yanagida T.

    Seibutsu Butsuri   41   S189   2001

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    DOI: 10.2142/biophys.41.S189_4

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  • Imaging of Single MyosinV and VI Moving along Actin Filaments

    Nishikawa S., Yanagida T., Homma K., Komori Y., Iwaki M., Tanaka H., Kitamura K., Iwane A., Ikebe R., Ikebe M.

    Seibutsu Butsuri   41   S29   2001

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    DOI: 10.2142/biophys.41.S29_1

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  • Single molecule nanometry of Myosin VI by optical tweezers : Single Myosin VI molecule moves processively along an actin filament

    Iwaki M., Nishikawa S., Komori Y., Tanaka H., Kitamura K., Iwane A., Ikebe R., Ikebe M., Yanagida T.

    Seibutsu Butsuri   41   S191   2001

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    DOI: 10.2142/biophys.41.S191_2

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  • Conformational changes of myosin V observed by single-molecule fluorometry

    Wazawa T., Ishii Y., Sugihara K., Iwane A., Homma K., Ikebe M., Yanagida T.

    Seibutsu Butsuri   41   S188   2001

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    DOI: 10.2142/biophys.41.S188_4

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  • The motor domain, not a long neck domain, is responsible for the large step and processivity of myosin V.

    Tanaka H., Homma K., Iwane A.H., Katayama E., Ikebe R., Saito J., Yanagida T., Ikebe M.

    Seibutsu Butsuri   41   S190   2001

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    DOI: 10.2142/biophys.41.S190_3

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  • Motion of oneheaded kinesin molecules without neck-linker along a microtubule

    Inoue Y., Azuma S., Iwane A.H., Yanagida T.

    Seibutsu Butsuri   41   S195   2001

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    DOI: 10.2142/biophys.41.S195_1

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  • Single molecule processes of Ras

    Arai Y., Yokota H., Iwane A.H., Wazawa T., Kataoka T., Ishii Y., Yanagida T.

    Seibutsu Butsuri   41   S217   2001

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    DOI: 10.2142/biophys.41.S217_1

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  • A large step for myosin Reviewed

    T Yanagida, AH Iwane

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   97 ( 17 )   9357 - 9359   2000.8

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  • Single-motor mechanics and models of the myosin motor

    T Yanagida, S Esaki, AH Iwane, Y Inoue, A Ishijima, K Kitamura, H Tanaka, M Tokunaga

    PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES   355 ( 1396 )   441 - 447   2000.4

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    DOI: 10.1098/rstb.2000.0585

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  • Direct inhibition of microtubule-based kinesin motility by local anesthetics Reviewed

    Y Miyamoto, E Muto, T Mashimo, AH Iwane, Yoshiya, I, T Yanagida

    BIOPHYSICAL JOURNAL   78 ( 2 )   940 - 949   2000.2

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  • Single molecule analysis of the actomyosin motor Reviewed

    T Yanagida, K Kitamura, H Tanaka, AH Iwane, S Esaki

    CURRENT OPINION IN CELL BIOLOGY   12 ( 1 )   20 - 25   2000.2

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    DOI: 10.1016/S0955-0674(99)00052-6

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  • Role of the myosin neck region in generation of movement

    H Tanaka, AH Iwane, T Okumura, S Morimoto, T Kusumoto, A Ishijima, T Yanaglda

    BIOPHYSICAL JOURNAL   78 ( 1 )   234A - 234A   2000.1

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  • Motion of a single molecule of catalytic core domain of kinesin along a microtubule in the presence of ADP

    Y Inoue, T Miyai, AH Iwane, T Yanagida

    BIOPHYSICAL JOURNAL   78 ( 1 )   122A - 122A   2000.1

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  • Myosin neck region does not act as a lever arm but rather regulates the kinetics of actin-myosin interaction.

    H Tanaka, K Kitamura, AH Iwane, T Yanagida

    BIOPHYSICAL JOURNAL   78 ( 1 )   3A - 3A   2000.1

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  • A single myosin head moves along an actin filament with regular-5 nm steps under loaded condition

    K Kitamura, AH Iwane, T Yanagida

    BIOPHYSICAL JOURNAL   78 ( 1 )   234A - 234A   2000.1

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  • Visualization of dimerization using fluorescence resonance energy transfer (FRET) imaging between biofluorescent proteins

    Iwane A.H., Wazawa T., Ishii Y., Inoue J., Yanagida T.

    Seibutsu Butsuri   40   S177   2000

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    DOI: 10.2142/biophys.40.S177_3

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  • Laser trapping nanometry with DIG DNA probe.

    Kimura Y., Iwane A., Tanaka H., Okamoto K., Ishijima A.

    Seibutsu Butsuri   40   S203   2000

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    DOI: 10.2142/biophys.40.S203_3

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  • Single molecule techniques in biophysics Reviewed

    T Yanagida, H Tanaka, K Kitamura, T Wazawa, M Nishiyama, S Esaki, Y Sako, T Ide, AH Iwane, Y Ishii

    NA/K-ATPASE AND RELATED ATPASES   1207   71 - 85   2000

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  • A single myosin head moves along an actin filaments with regular steps during one biochemical cycle of ATP hydrolysis.

    Kazuo KITAMURA, Atsuko H. IWANE, Makio TOKUNAGA

    Biophysics   40 ( 2 )   89 - 93   2000

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    To resolve the individual mechanical events of force generation by actomyosin, we have developed a new technique to capture and directly manipulate individual myosin subfragment-1 (S1) molecules using a scanning probe. The unitary mechanical step during sliding along an actin filament is 5.3nm, but 2 to 5 rapid steps in succession often end up with total displacements of &sim;11 to &sim;30nm. Multiple steps are produced by single heads during the hydrolysis of a single ATP molecule.

    DOI: 10.2142/biophys.40.89

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    Other Link: http://search.jamas.or.jp/link/ui/2000164740

  • The step size, processivity, and velocity of myosin V are independent on its neck length.

    Tanaka H, Honma K., Iwane A.H., Katayama E., Ikebe M., Yanagida T.

    Seibutsu Butsuri   40   S202   2000

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    DOI: 10.2142/biophys.40.S202_2

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  • Single molecule analysis of actomyosin motor : Load dependence

    Kitamura K., Hikikoshi-Iwane A., Yanagida T.

    Seibutsu Butsuri   40   S202   2000

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    DOI: 10.2142/biophys.40.S202_4

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  • Single molecule imaging of small G protein Ras

    Matsuura H., Arai Y., Yokota H., Iwane A., Ishii Y., Kataoka T., Yanagida T.

    Seibutsu Butsuri   40   S32   2000

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    DOI: 10.2142/biophys.40.S32_3

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  • Mechanical and chemical properties of cysteine-modified kinesin molecules Reviewed

    S Iwatani, AH Iwane, H Higuchi, Y Ishii, T Yanagida

    BIOCHEMISTRY   38 ( 32 )   10318 - 10323   1999.8

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    DOI: 10.1021/bi9904095

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  • A single myosin head moves along an actin filament with regular steps of 5.3 nanometres Reviewed

    Kazuo Kitamura, Makio Tokunaga, Atsuko Hikikoshi Iwane, Toshio Yanagida

    Nature   397 ( 6715 )   129 - 134   1999.1

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    DOI: 10.1038/16403

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  • Single-molecule mechanics of actomyosin measured by an optical tweezers ; Role of the neck region.

    Tanaka H., Iwane A., Okumura T., Morimoto S., Kusumoto T., Ishijima A., Yanagida T.

    Seibutsu Butsuri   39   S138   1999

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    DOI: 10.2142/biophys.39.S138_4

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  • A fuorescent spectroscopic study of ficrotubule dynamics.

    Muto E., Saito Y., Iwane A.H.K., Ishii Y.

    Seibutsu Butsuri   39   S144   1999

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    DOI: 10.2142/biophys.39.S144_1

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  • Filament structure as an essential factor for regulation of Dictyostelium myosin by regulatory light chain phosphorylation Reviewed

    Liu, X, K Ito, S Morimoto, A Hikkoshi-Iwane, T Yanagida, TQP Uyeda

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   95 ( 24 )   14124 - 14129   1998.11

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    DOI: 10.1073/pnas.95.24.14124

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  • Direct inhibitory effects of local anesthetics on microtubule associated motor protein kinesin - Molecular mechanisims of the inhibition of axoplasmic transport by local anesthetics -

    Y. Miyamoto, T. Mashimo, A. H. Iwane, I. Yoshiya, T. Yanagida

    Anesthesia and Resuscitation   34 ( 4 )   229 - 230   1998

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  • Direct inhibition effects of local anesthetics on microtuble associated motor protein kinesin : Molecular mechanisms of the inhibition of axoplasmic transport by local anesthetics Reviewed

    麻酔と蘇生   34: 229-230   1998

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  • Dual-colour microscopy of single fluorophores bound to myosin interacting with fluorescently labelled actin using anti-Stokes fluorescence Reviewed

    K Saito, M Tokunaga, AH Iwane, T Yanagida

    JOURNAL OF MICROSCOPY-OXFORD   188   255 - 263   1997.12

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    DOI: 10.1046/j.1365-2818.1997.2580814.x

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  • Movements of truncated kinesin fragments with a short or an artificial flexible neck Reviewed

    Y Inoue, YY Toyoshima, AH Iwane, S Morimoto, H Higuchi, T Yanagida

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   94 ( 14 )   7275 - 7280   1997.7

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  • Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy Reviewed

    M Tokunaga, K Kitamura, K Saito, AH Iwane, T Yanagida

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   235 ( 1 )   47 - 53   1997.6

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    DOI: 10.1006/bbrc.1997.6732

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  • Single molecular assay of individual ATP turnover by a myosin-GFP fusion protein expressed in vitro Reviewed

    AH Iwane, T Funatsu, Y Harada, M Tokunaga, O Ohara, S Morimoto, T Yanagida

    FEBS LETTERS   407 ( 2 )   235 - 238   1997.4

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    DOI: 10.1016/S0014-5793(97)00359-1

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  • Manipulation and nanometer measurement of a single motor protein molecule captured directly by a scanning probe.

    K Kitamura, M Tokunaga, A Iwane, K Saito, T Yanagida

    BIOPHYSICAL JOURNAL   72 ( 2 )   MP170 - MP170   1997.2

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  • Movements of truncated kinesin fragments with a short or an artificial flexible neck. Reviewed

    Y Inoue, S Morimoto, A Iwane, YY Toyoshima, H Higuchi, T Yanagida

    BIOPHYSICAL JOURNAL   72 ( 2 )   MP211 - MP211   1997.2

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  • Myosin subfragment-1 is fully equipped with factors essential for motor function Reviewed

    AH Iwane, K Kitamura, M Tokunaga, T Yanagida

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   230 ( 1 )   76 - 80   1997.1

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    DOI: 10.1006/bbrc.1996.5861

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  • Direct inhibitory effects of local anesthetics on microtubule associated motor protein kinesin investigated by in vitro motility assay and single- molecule imaging

    Y. Miyamoto, T. Mashimo, Y. Tsuda, A. H. Iwane, I. Yoshiya, T. Yanagida

    Anesthesia and Resuscitation   33 ( 4 )   249 - 250   1997

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  • Direct inhibition effects of local anesthetics on microtuble associated motor protein kinesin investigated by in vitro motility assay and single-molecule imagaing. Reviewed

    麻酔と蘇生   33: 249-250   1997

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  • Two-dimensional crystals of the Kdp-ATPase of Escherichia coli Reviewed

    AH Iwane, Ikeda, I, Y Kimura, Y Fujiyoshi, K Altendorf, W Epstein

    FEBS LETTERS   396 ( 2-3 )   172 - 176   1996.11

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    DOI: 10.1016/0014-5793(96)01096-4

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  • AGONISTIC-ANTAGONISTIC ACTIONS OF CLOMIPHENE CITRATE ON PGF2-ALPHA FLUCTUATIONS IN OVARIECTOMIZED RAT UTERUS Reviewed

    H KOGO, A HIKIKOSHI

    JAPANESE JOURNAL OF PHARMACOLOGY   58 ( 2 )   197 - 200   1992.2

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  • HIGH-LEVEL SECRETION OF THE EXTRACELLULAR DOMAIN OF THE HUMAN GROWTH-HORMONE RECEPTOR USING A BACULOVIRUS SYSTEM Reviewed

    Y OTA, A ASAKURA, Y MATSUURA, H KONDO, A HITOSHIO, A IWANE, T TANAKA, M KIKUCHI, M IKEHARA

    GENE   106 ( 2 )   159 - 164   1991.10

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    DOI: 10.1016/0378-1119(91)90195-H

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  • THE U5 SEQUENCE IS A CIS-ACTING REPRESSIVE ELEMENT FOR GENOMIC RNA EXPRESSION OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I Reviewed

    M SEIKI, A HIKIKOSHI, M YOSHIDA

    VIROLOGY   176 ( 1 )   81 - 86   1990.5

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    DOI: 10.1016/0042-6822(90)90232-G

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  • THYMIC ATROPHY CHARACTERISTIC IN TRANSGENIC MICE THAT HARBOR PX GENES OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I Reviewed

    Y FURUTA, S AIZAWA, Y SUDA, Y IKAWA, H KISHIMOTO, Y ASANO, T TADA, A HIKIKOSHI, M YOSHIDA, M SEIKI

    JOURNAL OF VIROLOGY   63 ( 7 )   3185 - 3189   1989.7

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  • THE P40X OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I IS A TRANS-ACTING ACTIVATOR OF VIRAL GENE-TRANSCRIPTION Reviewed

    M SEIKI, J INOUE, T TAKEDA, A HIKIKOSHI, M SATO, M YOSHIDA

    JAPANESE JOURNAL OF CANCER RESEARCH   76 ( 12 )   1127 - 1131   1985.12

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    DOI: 10.20772/cancersci1985.76.12_1127

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  • EXPRESSION OF THE PX GENE OF HTLV-I - GENERAL SPLICING MECHANISM IN THE HTLV FAMILY Reviewed

    M SEIKI, A HIKIKOSHI, T TANIGUCHI, M YOSHIDA

    SCIENCE   228 ( 4707 )   1532 - 1534   1985

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    DOI: 10.1126/science.2990031

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  • Cyanidioschzon merolae: A new Model Eukaryote for Cell and Organelle Biology

    Ichinose TM, Iwane AH

    Springer Singapore  2017  ( ISBN:9789811061011 )

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  • シグナル伝達研究2005-’06-第三章 シグナル伝達研究における新たなアプローチ-情報伝達分子の正細胞イメージング

    羊土社  2005 

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  • Motor function of unconventional myosin.

    Adv Exp Med Biol.  2003 

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  • エネルギー変換装置ATPaseの作動原理-分子機械の作動原理

    岩根敦子, 小森靖則, 岩城光宏, 柳田敏雄

    共立出版  2002 

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    Responsible for pages:蛋白質核酸酵素 47(9): 1157 -1164 2002  

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  • Studying Molecular Motors on the Single Molecule Level.

    Zander, Enderlein, Keller eds.: Single-Molecule Detection in Solution (WILE Y-VCH Verlag Berlin)  2002 

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  • 実験医学別冊 ポストゲノム時代の実験講座3- GFPとバイオイメージング-第四章 "GFPの検出およびイメージング技術"

    岩根 敦子

    羊土社  2000 

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    Responsible for pages:223-232  

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  • Single molecule techniques in biophysics.

    The proceedings of the 9th international conference on Na/K Pump and related pumps. ISC  2000 

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MISC

  • Analysis of novel gene which creates the orientation of collagen fibers supporting zebrafish fin tissues

    谷本凜太朗, 板橋岳志, 岩根敦子, 近藤滋, 黒田純平

    日本結合組織学会学術大会抄録集   56th   2024

  • ゼブラフィッシュ新規変異体を用いた,ヒレづくりにおいて骨組みとなるコラーゲン繊維の配向を形成する遺伝子の機能解析

    谷本凜太朗, 板橋岳志, 岩根敦子, 近藤滋, 黒田純平

    日本分子生物学会年会プログラム・要旨集(Web)   47th   2024

  • 宿主-ウイルス相互作用による小胞体膜変形の分子メカニズム

    徳田ちこ, 尾崎拓馬, 岩根敦子, 岩根敦子, 板橋岳志, 岡本徹, 伊藤祐美, 吉村成弘, 吉村成弘

    日本分子生物学会年会プログラム・要旨集(Web)   47th   2024

  • Molecular pathology of cilia-related signaling in peroxisome biogenesis disorders

    宮本達雄, 宮本達雄, 細羽康介, 板橋岳志, 岩根敦子, 阿久津シルビア夏子, 落合博, 斎藤裕見子, 山本卓, 松浦伸也

    日本分子生物学会年会プログラム・要旨集(Web)   44th   2021

  • 魚類ヒレの骨格形成に必須なコラーゲン繊維の配向をつくり出す細胞依存的な新規メカニズム

    黒田 純平, 板橋 岳志, 岩根 敦子, 近藤 滋

    日本結合組織学会学術大会プログラム・抄録集   52回   81 - 81   2020.9

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  • コレステロールの繊毛への輸送に関与する繊毛-ペロキシソーム伝達機構解明に向けた3D-CLEM(光-電子相関顕微鏡法)観察

    板橋岳志, 宮本達雄, 細羽康介, 細羽康介, 岩根敦子, 岩根敦子

    日本分子生物学会年会プログラム・要旨集(Web)   43rd   2020

  • ペルオキシソームは一次繊毛にコレステロールを供給する:ペルオキシソーム形成不全症Zellweger症候群からの知見

    宮本達雄, 細羽康介, 細羽康介, 板橋岳志, 岩根敦子, 岩根敦子, AKUTSU Silvia Natsuko, 落合博, 斎藤裕見子, 山本卓, 松浦伸也

    日本分子生物学会年会プログラム・要旨集(Web)   43rd   2020

  • 直線状に発達するヒレ骨の建築に必須なコラーゲン結晶のダイナミクス

    黒田純平, 黒田純平, 板橋岳志, 岩根敦子, 近藤滋

    日本分子生物学会年会プログラム・要旨集(Web)   43rd   2020

  • 魚類ヒレ骨の形態形成に必須なコラーゲン結晶の成長メカニズム

    黒田純平, 板橋岳志, 岩根敦子, 近藤滋

    日本分子生物学会年会プログラム・要旨集(Web)   42nd   2019

  • 尾びれ形成過程を先端電子顕微鏡解析から得られた3D構造モデルから読み解く

    板橋岳志, 板橋岳志, 板橋岳志, 一ノ瀬孝子, 黒田純平, 近藤滋, 岩根敦子, 岩根敦子, 岩根敦子

    日本分子生物学会年会プログラム・要旨集(Web)   42nd   2019

  • 3D-structual modeling of myogenic differentiation of C2C12 Myoblasts by advanced electron microscopy and light microscopy

    Takako M. Ichinose, Sei Saitoh, Atsuko H. Iwane

    Biophysical Journal,   114 ( 3 )   164A   2018.2

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    DOI: 10.1016/j.bpj.2017.11.920

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  • 先端電子顕微鏡を用いた1細胞丸ごとレベルでの細胞内小器官の3D微細構造モデル作成と生物組織への展望

    一ノ瀬孝子, 板橋岳志, 板橋岳志, 板橋岳志, 森ひかり, 黒田純平, 近藤滋, 岩根敦子, 岩根敦子, 岩根敦子

    日本分子生物学会年会プログラム・要旨集(Web)   41st   2018

  • Structural Visualization of Mitotic Cycle by Three-Dimensional Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) with Nanoscale Resolution at Whole Cell Level

    Rina Nagai, Keisuke Ohta, Atsuko H. Iwane

    BIOPHYSICAL JOURNAL   106 ( 2 )   600A - 600A   2014.1

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  • Single molecule detection of the two-legged nano-machine

    NISHIKAWA So, SUGAWA Mitsuhiro, KOMORI Tomotaka, MORIMATSU Masatoshi, IWANE Atsuko H., YANAGIDA Toshio

    2008 ( 1 )   5 - 8   2008.2

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  • Coiled-coil domain of myosin V

    Takayuki Ariga, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida

    BIOPHYSICAL JOURNAL   494A - 494A   2007.1

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  • 2P125 Single myosin V tracking with improved time resolution(Molecular motors,Poster Presentations)

    Nishikawa So, Sugawa Mitsuhiro, Ueno Hiroshi, Iwane Atsuko H, Yanagida Toshio

    Seibutsu Butsuri   47 ( 0 )   S144   2007

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    DOI: 10.2142/biophys.47.S144_2

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  • Biased diffusion model driven by a single-headed myosin.

    Tanaka H, Iwaki M, Iwane AH, Ikebe M, Yanagida T

    Biophysical Journal   90   2006

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    DOI: 10.1529/biophysj.105.075721

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  • Direct Observation of molecular recognition between cell adhesion molecules using flexible glass microneedle.

    Tsukasaki Y, Kitamura K, Simizu K, Iwane AH, Takai Y, Yanagida T

    Biophysical Journal   90   2006

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  • Development of a novel microscope for simultaneous observation of mechano-chemical coupling.

    Ariga T, Komori T, Nishikawa S, Iwane AH, Yanagida T

    Biophysical Journal   90   2006

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  • Processivity of cargo bound single-headed wild-type myosin-VI.

    Iwaki M, Tanaka H, Iwane AH, Ikebe M, Yanagida T

    Biophysical Journal   90   2006

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  • Single Myosin-V and-VI head step along the actin filament with ~ 5.5 nm steps”.

    Okada T, Kitamura K, Tanaka H, Iwane AH, Ikebe M, Yanagida T

    Biophysical Journal   90   2006

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  • Further Characterization of Novel Crossbridge Conformation During Actin-Sliding.

    Katayama E, Kimori Y, Oguchi Y, Iwane AH, Yanagida T, Baba N

    Biophysical Journal   90   2006

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  • 【シグナル伝達研究2005-'06 生命現象や疾患を支配する分子メカニズムと新しい研究法 現在と未来】 シグナル伝達研究における新たなアプローチ 情報伝達分子の生細胞イメージング

    岩根敦子, 上田昌宏, 日比野佳代, 佐甲靖志, 柳田敏雄

    実験医学   23 ( 11 )   1796 - 1802   2005.6

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  • 高感度分子間力顕微鏡による生体分子間ナノ反応場の1分子直接観察

    塚崎克和, 喜多村和郎, 清水一也, 岩根敦子, 高井義美, 柳田敏雄

    ナノ学会大会講演予稿集   3rd   164   2005.5

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    J-GLOBAL

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  • 高感度1分子相互作用計測により検出された細胞間接着分子ダイナミクス

    塚崎克和, 喜多村和郎, 清水一也, 岩根敦子, 高井義美, 柳田敏雄

    日本分子生物学会年会プログラム・講演要旨集   27th   340   2004.11

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    J-GLOBAL

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  • 14aPS-82 How Myosin VI May Walk

    Ali Yusuf M., Homma K., Iwane A., Adachi K., Itoh H., Kinosita K. Jr., Yanagida T., Ikebe M.

    Meeting abstracts of the Physical Society of Japan   59 ( 2 )   258 - 258   2004.8

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  • Single-molecule analysis reveals multiple binding sites of Raf1 on the plasma membrane

    Hibino K, Sako Y, Iwane A, Yanagida T

    Seibutsu Butsuri   43 ( 0 )   S157   2003

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    DOI: 10.2142/biophys.43.S157_2

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  • The motor domain determines the large step of myosin-V

    H Tanaka, K Homma, AH Iwane, E Katayama, R Ikebe, J Saito, T Yanagida, M Ikebe

    NATURE   415 ( 6868 )   192 - 195   2002.1

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  • 1N0900 Single-molecule imaging of H-Ras and cRaf-1 on the plasma membrane of living cells

    Hibino K, Sako Y, Iwane A. H, Yanagida T

    Seibutsu Butsuri   42 ( 2 )   S80   2002

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    DOI: 10.2142/biophys.42.S80_1

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  • Studying Molecular Motors on the Single Molecule Level.

    Zander, Enderlein, Keller eds.: Single-Molecule Detection in Solution (WILE Y-VCH Verlag Berlin)   p. 273-292, Chapter 9   2002

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  • The motor domain. Not a long neck domain, determines the large step of Myosin V

    T Yanagida, H Tanaka, K Homma, AH Iwane, E Katayama, R Ikebe, J Saito, M Ikebe

    JOURNAL OF GENERAL PHYSIOLOGY   118 ( 1 )   4A - 4A   2001.7

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  • The motor domain, not a long neck domain, determines the large step of myosin V.

    H Tanaka, K Homma, AH Iwane, E Katayama, R Ikebe, J Saito, T Yanagida, M Ikebe

    BIOPHYSICAL JOURNAL   80 ( 1 )   81A - 81A   2001.1

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  • Signal transduction from Ras to cRaf-1 in EGF-stimulated cells

    Hibino K, Sako Y, Iwane A.H, Kataoka T, Yanagida T

    Seibutsu Butsuri   41 ( 0 )   S122   2001

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    DOI: 10.2142/biophys.41.S122_2

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  • Spatiotemporal dynamics of Ras/cRaf-1 interaction in the plasma membrane of living cells

    Hibino K, Sako Y, Iwane A.H, Okada T, Kataoka T, Yanagida T

    Seibutsu Butsuri   40 ( 0 )   S76   2000

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    DOI: 10.2142/biophys.40.S76_1

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  • Characterization of cysteine-free and cysteine-remained kinesin.

    AH Iwane, S Iwatani, H Higuchi, K Shima, Y Ishii, T Yanagida

    BIOPHYSICAL JOURNAL   76 ( 1 )   A44 - A44   1999.1

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  • S1 attached to a glass surface through a flexible random chain can move actin as fast as intact myosin

    M Tokunaga, A Iwane, K Kitamura, T Yanagida

    BIOPHYSICAL JOURNAL   76 ( 1 )   A36 - A36   1999.1

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  • Single molecule imaging of one-headed kinesin molecules interacting with a microtubule.

    Y Inoue, AH Iwane, T Yanagida

    BIOPHYSICAL JOURNAL   76 ( 1 )   A44 - A44   1999.1

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  • A single myosin read moves along an actin filament with regular steps of similar to 5.5 nm.

    K Kitamura, M Tokunaga, AH Iwane, T Yanagida

    BIOPHYSICAL JOURNAL   76 ( 1 )   A36 - A36   1999.1

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  • Ras mediated recruitment of Raf-1 to plasma membrane visualized in living cells.

    Hibino K, Sako Y, Iwane A, Kataoka T, Yanagida T

    Seibutsu Butsuri   39 ( 0 )   S51   1999

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    DOI: 10.2142/biophys.39.S51_1

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  • 骨格筋ミオシンS1-GFP融合タンパクの試験管内発現と1分子アッセイ

    Cytometry Research   9 :11-20   1999

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  • 微小管モーター蛋白質キネシンに対する局所麻酔薬の直接阻害作用(II) 局所麻酔薬による軸索輸送阻害作用の分子メカニズム

    宮本 善一, 真下 節, 岩根 敦子, 吉矢 生人, 柳田 敏雄

    麻酔と蘇生   34 ( 4 )   229 - 230   1998.12

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    局所麻酔薬は主にその荷電型が,キネシンのATPase活性を阻害することなく,その運動能を直接・用量依存性・可逆的に阻害する.これが,局所麻酔薬による軸索輸送阻害作用の主因と考えられる

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  • Motility of kinesin-ncd fusion proteins.

    Miyai T., Inoue Y., Hibino K., Iwane A.H., Yanagida T.

    Biophysics   38 ( 2 )   S75   1998.9

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  • Dfrect observation of the stepping motion of a single myosin head.

    Kitamura K., Tokunaga M., Iwane A., Yanagida T.

    Biophysics   38 ( 2 )   S127   1998.9

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  • Myosin Subfragment-1, attached to a glass surface through a flexible chain, can move actin as fast as native myosin.

    Iwane A.H., Kitamura K., Yanagida T., Tokunaga M.

    Biophysics   38 ( 2 )   S128   1998.9

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  • Laser trapping nanometry and imaging of single kinesin molecules moving along microtubules.

    Nonoyama Y., Inoue Y., Nishiyama M., Miyai T., Shima K., Iwane A., Yanagida T.

    Biophysics   38 ( 2 )   S93   1998.9

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  • Single molecule imaging of one-headed kinesin molecules interacting with a microtubule.

    Inoue Y., Iwane A.H., Yanagida T.

    Biophysics   38 ( 2 )   S75   1998.9

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  • Characterization of Cysteine-free and Cysteine-remained kinesin

    Shima K., Iwatani S., Iwane H., Inoue Y., Higuchi H., Ishii Y., Yanagida T.

    Biophysics   38 ( 2 )   S75   1998.9

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  • Single-molecule mechanics of mutant myosin measured by an optical tweezers

    Tanaka H., Iwane A., Kusumoto T., Yanagida T.

    Biophysics   38 ( 2 )   S127   1998.9

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  • Direct inhibition of the microtubule-based kinesin motility by local anesthetics

    Y Miyamoto, T Mashimo, AH Iwane, Yoshiya, I, T Yanagida

    ANESTHESIOLOGY   89 ( 3A )   U1068 - U1068   1998.9

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  • 緑色蛍光蛋白質(GFP)の細胞生物学への応用 骨格筋ミオシンS1-GFPキメラ蛋白の試験管内発現と1分子機能アッセイ

    岩根 敦子

    Cytometry Research   8 ( Suppl. )   34 - 34   1998.6

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  • Myosin step size determined by directly manipulating a single S1 molecule with a scanning probe

    K Kitamura, M Tokunaga, AH Iwane, T Yanagida

    BIOPHYSICAL JOURNAL   74 ( 2 )   A265 - A265   1998.2

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  • Single molecular assay of the individual ATP turnovers by a myosin-GFP fusion protein expressed in vitro

    AH Iwane, T Funatsu, Y Harada, M Tokunaga, O Ohara, T Yanagida

    BIOPHYSICAL JOURNAL   74 ( 2 )   A260 - A260   1998.2

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  • Expression of dictyostelium discoideum myosin head and chiken skeletal muscle myosinfusion protein

    MORIMOTO S., OKUMURA T., TANAKA H., ISHIJIMA A., IWANE A., YANAGIDA T.

    Biophysics   37   S136   1997.10

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  • Laser trapping nanometry for single kinesin molecules

    INOUE Y., HIGUCHI H., NISHIYAMA M., IWANE A. H., IWATANI S., MIYAI T., YANAGIDA T.

    Biophysics   37   S76   1997.10

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  • Single molecule manipulation and nanometry of myosin subfagment-I

    KITAMURA K., TOKUNAGA M., IWANE A., YANAGIDA T.

    Biophysics   37   S210   1997.10

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  • Movements of truncated motor domains of kinesin

    INOUE Y., MIYAI T., IWANE A. H., HIGUCHI H., YANAGIDA T.

    Biophysics   37   S216   1997.10

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  • Development of the system for detecting steps by single molecules of one-headed kinesin

    INOUE Y., HIGUCHI H., IWANE A. H., YANAGIDA T.

    Biophysics   37   S216   1997.10

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    CiNii Article

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  • Direct effect of local anesthetics on kinesin-microtubule motility investigated by single-molecule imaging

    MIYAMOTO Y., MASHIMO T., H. IWANE A., YOSHIYA I., YANAGIDA T.

    Biophysics   37   S118   1997.10

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  • Objective-type total internal reflection fluorescence microscopy and single molecule imaging of ATPase reactions

    TOKUNAGA M., KITAMURA K., SAITO K., H. IWANE A., YANAGIDA T.

    Biophysics   37   S53   1997.10

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  • Preparation of cysteine-lesskinesin

    IWATANI S., IWANE A., SHIMA K., HIGUCHI H., YANAGIDA T.

    Biophysics   37   S205   1997.10

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  • Individual ATPase assay and in vitro expression of myosin-GFP fused protein

    H. IWANE A., FUNATSU T., HARADA Y., OHARA O., YANAGIDA T.

    Biophysics   37   S211   1997.10

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  • Fluctuation of myosin conformation detected by single fluorophore polarization measurement

    SAITO K., TOKUNAGA M., H. IWANE A., YANAGIDA T.

    Biophysics   37   S212   1997.10

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  • Cooperative binding of kinesin molecules to a microtubule in the presence of ATP

    MUTO E., MIYAMOTO Y., FUNATSU T., HARADA Y., IWANE A., YANAGIDA T.

    Biophysics   37   S214   1997.10

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  • Polarization measurements of single fluorophores attached to motor protein in aqueous solution

    K Saito, M Tokunaga, A Iwane, T Yanagida

    BIOPHYSICAL JOURNAL   72 ( 2 )   TU238 - TU238   1997.2

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  • Myosin subfragment-1 (S1) is fully equipped with factors essential for motor function as well as native myosin.

    A Iwane, K Kitamura, M Tokunaga, T Yanagida

    BIOPHYSICAL JOURNAL   72 ( 2 )   MP172 - MP172   1997.2

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  • Single molecule imaging of one-headed kinesin molecules bound to a bead

    Inoue Y, Higuchi H, Iwane AH, Yanagida T

    Biophysical Journal   74   238   1997

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  • ニワトリ大人型骨格筋ミオシンS1のクローニングと発現

    森本 さゆり, 岩根 敦子, 柳田 敏雄

    日本分子生物学会年会プログラム・講演要旨集   19   640 - 640   1996.8

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  • Trapping a single protein molecule onto a probe.

    喜多村和郎, 徳永万喜洋, 岩根敦子, 船津高志, 柳田敏雄

    日本生物物理学会年会講演予稿集   33rd   S154   1995.8

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    J-GLOBAL

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  • AGONISTIC-ANTAGONISTIC ACTIONS OF CLOMIPHENE CITRATE ON PGF2-ALPHA FLUCTUATIONS IN OVARIECTOMIZED RAT UTERUS

    H KOGO, A HIKIKOSHI

    JAPANESE JOURNAL OF PHARMACOLOGY   58 ( 2 )   197 - 200   1992.2

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  • THYMIC ATROPHY CHARACTERISTIC IN TRANSGENIC MICE THAT HARBOR PX GENES OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I

    Y FURUTA, S AIZAWA, Y SUDA, Y IKAWA, H KISHIMOTO, Y ASANO, T TADA, A HIKIKOSHI, M YOSHIDA, M SEIKI

    JOURNAL OF VIROLOGY   63 ( 7 )   3185 - 3189   1989.7

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  • THE P40X OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I IS A TRANS-ACTING ACTIVATOR OF VIRAL GENE-TRANSCRIPTION

    M SEIKI, J INOUE, T TAKEDA, A HIKIKOSHI, M SATO, M YOSHIDA

    JAPANESE JOURNAL OF CANCER RESEARCH   76 ( 12 )   1127 - 1131   1985.12

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    DOI: 10.20772/cancersci1985.76.12_1127

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Presentations

  • THE ELUCIDATION OF THE FORMATION PROCESS OF ZEBRAFISH TAIL FIN BY 3D MODEL USING ADVANCED TRANS-SCALE EM AND BY CLEM.

    Junpei Kuroda, Takeshi Itabashi, Takako Ichinose, Shigeru Kondo, Atsuko H.Iwane

    64th Annual Meeting of the Biophysical Society  2020.2.16 

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  • p53によるDNA損傷刺激誘導性Fアクチン構造の制御とその意義

    伊藤功彦, 平田 宏聡, 杉本 渉, 島本勇太, 岩根 敦子, 田中 信之, 川内 敬子

    第42回日本分子生物学会年会  2019.12.5 

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  • DNA損傷応答における核内アクチンフィラメント構造に対するp53の役割

    川内 敬子, 伊藤 功彦, 杉本 渉, 取井 猛流, 岩根 敦子, 島本 勇太, 平田 宏聡

    第42回日本分子生物学会年会  2019.12.5 

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  • Role of collagen crystals as a scaffold for fin-ray bone formation

    Kaho Tobiishi, Junpei Kuroda, Atsuko H. Iwane, Shigeru Kondo

    2019.12.4 

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  • 尾びれ形成過程を先端電子顕微鏡解析から得られた3D構造モデルから読み解く

    板橋 岳志, 一ノ瀬 孝子, 黒田 純平, 近藤 滋, 岩根 敦子

    第42回日本分子生物学会年会  2019.12.3 

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  • リボソームRNAの転写阻害による核小体崩壊メカニズムの解明

    杉本 渉, 豊田 駿, 建石 寿枝, 杉本 直己, 岩根 敦子, 三好 大輔, 川内 敬子

    第42回日本分子生物学会年会  2019.12.3 

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  • 魚類ヒレ骨の形態形成に必須なコラーゲン結晶の成長メカニズム

    黒田 純平, 板橋 岳志, 岩根 敦子, 近藤 滋

    第42回日本分子生物学会年会  2019.12.3 

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  • Attempt to understand the cellular function during developmental process from 3D structural model International conference

    Junpei Kuroda, Takeshi Itabashi, Takako Ichinose, Shigeru Kondo, Atsuko H. Iwane

    The 57th Annual Meeting of The Biophysical Society of Japan  2019.9.26  The Biophysical Society of Japan

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    Venue:Miyazaki, Japan  

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  • Realization of development processes from the 3D structural model that is obtained from advanced midroscope images. International conference

    2019.6.17  The Japanese Society of Microscopy

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    Venue:Nagoya Congress Center, Nagoya  

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  • Ultrastructural analysis of stretch-induced changes in dynamics of the cell nucleus. International conference

    The 75th Annual Meeting of the Japanese Society of Microscopy  2019.6.17  The Japanese Society of Microscopy

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    Venue:Nagoya Congress Center, Nagoya  

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  • 3D-structual modeling of differentiation and developmental process using advanced electron microscopy and light microscopy. International conference

    Takako Ichinose, Takeshi Itabashi, Hikari Mori, Junpei Kuroda, Masaki Imayasu, Sei Saitoh, Shigeru Kondo, Atsuko H. Iwane

    63th Annual Meeting of The Biophysical Society  2019.3.6  Biophysical Society

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    Venue:Baltimore Convention Center (Baltimore, USA)  

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  • Attempt to understand cell division, defferentiated, developmental process

    Takako M. Ichinose, Takashi Itabashi, Hikari Mori, Junpei Kuroda, Shigeru Kondo, Atsuko H. Iwan

    2018.9 

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  • 単細胞紅藻C. merolaeにおけるタイムラプスイメージング系の構築

    一ノ瀨 孝子, 岩根 敦子

    日本植物学会第82回大会  2018.9 

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  • Multiplexed HyperCEST detection of genetically-reconstituted gas vesicle na

    MIZUSHIMA Ryota, INOUE Kanako, NAGAI Rina, IWANE Atsuko, WATANABE Tomonobu, KIMURA Atusomi

    2018.9 

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  • 先端顕微鏡を用いて細胞分裂過程や細胞分化過程を読み解く

    岩根 敦子

    日本植物学会第82回大会  2018.9 

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  • 先端電子顕微鏡計測技術の医療及び産業への応用 Invited

    岩根 敦子

    平成30年度第1回大学等シーズ活用セミナー  2018.6.30  (公財)くれ産業振興センター

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    Venue:呉市、広島  

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  • 細胞分裂並びに分化過程を先端顕微鏡解析から得られた3D構造モデルから読み解く

    岩根 敦子

    日本顕微鏡学会第74回学術講演会  2018.5 

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  • 細胞分裂並びに分化過程を先端電子顕微鏡像から得られた3D構造モデルから読み解く

    岩根 敦子

    生命科学系学会合同年次大会  2017.12 

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  • 3D visualization of the precise location of symbiotic organelle crosstalk throughout mitosis in the primitive unicellular eukaryotic cell, C. merolae. International conference

    Atsuko H. Iwane

    Biophysical Society 61st Annual Meeting  2017.2  Biophysical Society

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    Venue:New Orleans  

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  • Construction of the imaging system to elucidate the process by which cytoskeletal proteins acquire function using primitive eukaryotic cell. International conference

    Takako M. Ichinose, Atsuko H. Iwane

    Biophysical Society 61st Annual Meeting  2017.2  Biophysical Society

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    Venue:New Orleans  

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  • 先端電子顕微鏡/光学顕微鏡の特性を活かした細胞丸ごとレベルでの3次元微細構造解析

    岩根 敦子

    東京工業大学セミナー  2017.1.30  東京工業大学

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    Venue:東京都  

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  • 宿主細胞内における内部共生した細胞小器官が主導する機能を明らかにする試み

    一ノ瀬(三室)孝子, 岩根敦子

    第39回日本分子生物学会年会  2016.11  日本分子生物学会

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    Venue:パシフィコ横浜(神奈川県横浜市)  

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  • New obvious information obtained from cell organelle 3D-structural models of primitive eukaryote.

    Atsuko H. Iwane, Rina Nagai, Hikari Mori, Takako M. Ichinose

    2016.11 

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  • 先端電子顕微鏡/光学顕微鏡それぞれの特性を活かして細胞丸ごとレベルでの三次元微細構造解析をおこなうことで明らかになったこと

    永井里奈, 森ひかり, 一ノ瀬(三室)孝子, 岩根敦子

    第39回日本分子生物学会年会  2016.11  日本分子生物学会

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    Venue:横浜市  

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  • 3D-microstructural visualization of the simplest eukaryotic cell during mitosis process using several cutting-edge microscopic techniques.

    Atsuko H. Iwane

    1st Cyanidioschyzon merolae Symposium  2016.7.4 

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  • 原始真核生物シゾンの有糸分裂過程を先端顕微鏡と3次元構造解析法で可視化する

    岩根 敦子

    日本顕微鏡学会第72回学術講演会  2016.6  日本顕微鏡学会

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    Venue:仙台市  

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  • 先端電子顕微鏡により細胞分裂過程を一細胞丸ごとレベルで可視化する

    岩根 敦子

    医学・生物学系共同利用研究報告会  2016.3  大阪大学超高圧顕微鏡センター

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    Venue:吹田市  

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  • 3D Microstructual visualization of the simplest of eukaryotic cell (Cyanidioschyzon merolae) during mitosis process using several new microscopic techniques. International conference

    A. H. Iwane

    Biophysical Society 60th annual meeting  2016.2 

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    Venue:Los Angels,California, U.S.A.  

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  • 新顕微鏡技術の特性を活かし単細胞紅藻シゾンの有糸分裂過程を三次元微細構造解析する

    永井 里奈, 太田 啓介, 一ノ瀬 孝子, 森 ひかり, 岩根 敦子

    2015.12 

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  • 単細胞紅藻の有糸分裂過程を新顕微鏡技術を活かし三次元微細構造解析する

    岩根 敦子

    SSSEM研究部会&生理研研究会 合同ワークショップ  2015.11  SEM連続断面観察による生物組織三次元再構築研究部会

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    Venue:愛知県岡崎市  

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  • New obvious information obtained from the cell size and shape during mitosis cycle

    Rina Nagai, Keisuke Ohta, Takako M. Ichinose, Hikari Mori, Atsuko H. Iwane

    The 56th Annual Meeting of the Biophysical Society of Japan  2015.9 

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  • 3D microstructural visualization of mitosis using Focused Ion Beam-Scanning Electron Microscope and 3MV Ultra-High Voltage Electron Microscope Tomography with nanoscale resolution International conference

    Atsuko H. Iwane, Keisuke Ohta

    GRC: Three Dimensional Electron Microscopy  2015.6  Gordon Research Conferences

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    Venue:New London NH United States  

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  • 3D microstructural visualization of mitosis with nanoscale resolution at wh

    IWANE Atsuko

    2015.5 

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  • Three-dimensional microstructural visualization of mitosis using Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) and 3MV Ultra-High Voltage Electron Microscope (UHVEM) Tomography with nanoscale resolution at whole cell level. International conference

    Atsuko H. Iwane, Keisuke Ohta

    Biophysical Society 59th Annual Meeting  2015.2 

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    Venue:Baltimore, MA, USA  

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  • Single Cell 3D structural analysis.

    Atsuko H. Iwane

    2014.11 

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  • The challenge to intact cell 3D-imaging by dual-axis Cryo-electron tomography and correlative light imaging.

    Takako M. Ichinose, Ruriko Ogawa, Rina Nagai, Atsuko H. Iwane

    2014.11 

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  • Three-dimensional microstructural visualization of mitosis using Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) with nanoscale resolution at whole cell level

    Rina Nagai, Keisuke Ohta, Takako M. Ichinose, Akinobu Togo, Atsuko H. Iwane

    2014.11 

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  • The challenge to intact cell 3D-imaging by dual-axis Cryo-electron tomography and correlative light imagingmaging of live cell organelles by Cryo-electron tomography and STEM

    Ruriko Ogawa, Takako M. Ichinose, Rina Nagai, Kazuhiro Aoyama, Atsuko H. Iwane

    2014.9 

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  • Visualization of cell cycle by three-dimensional FIB-SEM with nanoscale resolution at whole cell level

    Rina Nagai, Keisuke Ohta, Takako M. Ichinose, Akinobu Togo, Atsuko H. Iwane

    2014.9 

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  • Single Cell 3D structural analysis

    IWANE Atsuko

    2014.8 

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  • Visualization of cell cycle by three-dimensional FIB-SEM with nanoscale resolution at whole cell level. International conference

    Atsuko H. Iwane, Keisuke Ohta

    2014.7 

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  • 今まで見えなかった細胞の超微細構造のラビリンスを探検できたらおもろい

    岩根 敦子

    大阪大学超高圧センターセミナー  2014.4.2  大阪大学超高圧センター

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    Venue:大阪府吹田市  

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  • Structural visualization of mitotic cycle by three-dimensional Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) with nanoscale resolution at whole cell level International conference

    Rina Nagai, Keisuke Ohta, Atsuko H. Iwane

    Biophysical Society 58th Annual Meeting  2014.2.17  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • Visualization of cell cycle by three-dimensional focused ion beam-scanning electron microscope (FIB-SEM) with nanoscale resolution at whole cell level.

    Rina Nagai, Keisuke Ohta, Akinobu Togo, Atsuko H. Iwane

    2013.12.4 

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  • Imaging of live cell orgamelles by Cryo-electron tomography and STEM.

    Ruriko Ogawa, Kazuhiro Aoyama, Rina Nagai, Atsuko H. Iwane

    2013.10.28 

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  • Whole cell structure reconstruction by three-dimensional focused ion beam and scanning electron microscopy.

    Rina Nagai, Keisuke Ohta, Kazuhiro Aoyama, Akinobu Togo, Akihiro Kawamoto, Atsuko H. Iwane

    2013.10.28 

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  • Development for dynamic live cell imaging by cryo-electron tomography and STEM. International conference

    Atsuko H. Iwane, Ruriko Ogawa, Rina Nagai, Akihiro Kawamoto, Kazuhiro Aoyama

    Biophysical Society 57th Annual Meeting  2013.2.4  Biophysical Society

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    Venue:Philadelphia, PE, USA  

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  • 今まで見えなかった細胞の超微細構造のラビリンスを探検できたらおもろい Invited

    岩根 敦子

    東京理科大学理工学部  2013.1 

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    Venue:千葉県野田市  

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  • A step towards the structural analysis of ERMES in the cell by FIB-SEM tomography International conference

    Akihiro Kawamoto, Keisuke Ohta, Akinobu Togo, Kazuhiro Aoyama, Atsuko H. Iwane, Keiichi Namba, Toshio Yanagida

    Nagoya Symposium Frontiers in Structural Physiology  2013.1 

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    Venue:Nagoya, Aichi  

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  • Development for dynamic live cell imaging by cryo-electron tomography and STEM. International conference

    Atsuko H. Iwane, Ruriko Ogawa, Kazuhiro Aoyama

    Nagoya Symposium Frontiers in Structural Physiology  2013.1 

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    Venue:Nagoya, Aichi  

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  • Development for dynamic Live Cell imaging by Cryo-electron tomography and Scanning TEM

    Atsuko H. Iwane, Ruriko Ogawa, Rina Nagai, Akihiro Kawamoto, Kazuhiro Aoyama

    2012.12.13 

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  • アクトミオシン動作メカニズムを1分子ナノ計測技術で解明する Invited

    IWANE Atsuko

    2012.12.8 

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  • アクトミオシン動作メカニズム解明に向けて−ミオシンサブグループの特性を1分子ナノ計測で調べる− Invited

    岩根 敦子

    2012.11.7  東京大学基礎科学研究科

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    Venue:東京都渋谷区  

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  • Development for dynamic Live Cell imaging by cryo-electron tomography

    Atsuko H. Iwane, Ruriko Ogawa, Rina Nagai, Akihiro Kawamoto, Kazuhiro Aoyama

    Biophysical Society 50th Annual Meeting  2012.9.22 

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  • アクトミオシン動作メカニズムを1分子ナノ計測技術で解明する Invited

    岩根 敦子

    2012.8.20  久留米大学医学部

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  • OBSERVATION of SEQUENTIAL TRANSITION STATE in SKELETAL MUSCLE ACTIN FILAMENT depending on MYOSIN using smFRET and STEM TOMOGRAPHY in living cell. International conference

    Atsuko H. Iwane, Masatoshi Morimatsu, Kazuhiro Aoyama, Toshio Yanagida

    Gordon Research Conferences: Single Molecule Approaches to Biology  2012.7.16  Gordon Research

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    Venue:West Dover, VT, USA  

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  • Simultaneous observation of the three-dimensional orientation and position of a single fluorescent probe International conference

    Masashi Omachi, Yoshinori Komori, Atsuko H. Iwane, Fumihiko Fujii, Takashi Jin, Toshio Yanagida

    Biophysical Society 56th Annual Meeting  2012.2.29  Biophysical Society

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    Venue:SanDiego, CA, USA  

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  • The Adjacent binding state enables myosin VI dual function International conference

    Keigo Ikezaki, Tomotaka Komori, Mitsuhiro Sugawa, So Nishikawa, Yoshiyuki Arai, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 56th Annual Meeting  2012.2.28  Biophysical Society

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    Venue:SanDiego, CA, USA  

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  • Single molecule measurement of the myosin V energy transductiom process International conference

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 56th Annual Meeting  2012.2.28  Biophysical Society

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    Venue:SanDiego, CA, USA  

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  • Observation of sequential transition states in skeletal muscle actin filament in response to myosin using smFRET

    Atsuko H. Iwane, Masatoshi Morimatsu, Toshio Yanagida

    2011.12.16 

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  • Cryo STEM Tomography for Cell Biology Invited

    Kazuhiro Aoyama, Ruriko Ogawa, Atsuko H. Iwane

    2011.12.1 

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  • High resolution structural analysis of the actin - myosin rigor complex by CryoEM

    Takashi Fujii, Atsuko H. Iwane, Toshio Yanagida, Keiichi Namba

    Biophysical Society 49th Annual Meeting  2011.9.18  Biophysical Society of Japan

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    Venue:Himeji, Hyogo  

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  • imultaneous observation of myosin V three-dimensional orientation and linear steps

    Masashi Ohmachi, Yasunori Komori, Atsuko H. Iwane, Fumihiko Fujii, Toshio Yanagida

    Biophysical Society 49th Annual Meeting  2011.9.18  Biophysical Society of Japan

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    Venue:Himeji, Hyogo  

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  • Contribution of the lever arm to myosin VI’s dual function as a transporter and an anchor

    Keigo Ikezaki, Tomotaka Komori, Mitsuhiro Sugawa, Yoshiyuki Arai, So Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 49th Annual Meeting  2011.9.18  Biophysical Society of Japan

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    Venue:Himeji, Hyogo  

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  • Multiple actin structures in the G-F transformation

    Masatoshi Morimatsu, Yuichi Togashi, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 49th Annual Meeting  2011.9.18  Biophysical Society of Japan

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    Venue:Himeji, Hyogo  

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  • Single molecule measurement of the myosin V energy transduction process

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 49th Annual Meeting  2011.9.18  Biophysical Society of Japan

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    Venue:Himeji, Hyogo  

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  • Observation of sequential transition states in skeletal muscle actin filament in response to myosin using smFRET International conference

    Atsuko H. Iwane, Masatoshi Morimatsu, Toshio Yanagida

    Gordon Research Conferences: Muscle & Molecular Motors  2011.7.11  Gordon Research

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    Venue:New London, NH, USA  

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  • Brownian search-and-catch mechanism for myosin V and VI International conference

    Mitsuhiro Iwaki, Atsuko H. Iwane, Tetsuya Simokawa, Toshio Yanagida

    Gordon Research Conferences: Muscle & Molecular Motors  2011.7.11  Gordon Research

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    Venue:New London, NH, USA  

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  • Development of single molecule Nano-measurement Invited International conference

    Atsuko H. Iwane

    Northwestern University Feinberg School of Medicine  2011.7.9  Northwestern University Feinberg School of Medicine

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    Venue:Chicago, IL, USA  

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  • Observation of dynamical conformational changes of skeletal muscle actin filament International conference

    Atsuko H. Iwane, Masatoshi Morimatsu, Toshio Yanagida

    Biophysical Society 55th Annual Meeting  2011.3.5  Biophysical Society

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    Venue:Baltimore, MD, USA  

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  • Observation of mechanical process for myoin-II by a dark-field microscopy combined with an optical trap International conference

    Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 55th Annual Meeting  2011.3  Biophysical Society

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    Venue:Baltimore, MD, USA  

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  • Multiple actin structures in monomeric and filamentous states International conference

    Masatoshi Morimatsu, Yuichi Togashi, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 55th Annual Meeting  2011.3  Biophysical Society

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    Venue:Baltimore, MD, USA  

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  • Three dimensional orientation measurements of single fluorescence molecules by molecules by newly developed polarized fluorescence microscopy International conference

    Masashi Omachi, Yoshinori Komori, Atsuko H. Iwane, Fumihiko Fujii, Toshio Yanagida

    Biophysical Society 55th Annual Meeting  2011.3  Biophysical Society

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    Venue:Baltimore, MD, USA  

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  • Visualization and clarifying the mechanical properties of the myosin V brownian search with optical tweezers International conference

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 55th Annual Meeting  2011.3  Biophysical Society

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    Venue:Baltimore, MD, USA  

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  • The tail domain of myosin-VI ensures the directed processive movement International conference

    Keigo Ikezaki, Tomotaka Komori, Mitsuhiro Sugawa, So Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 55th Annual Meeting  2011.3  Biophysical Society

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    Venue:Baltimore, MD, USA  

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  • 量子ロッドを用いたミオシンV の3 次元配向計測

    大町優史, 小森靖則, 岩根敦子, 藤井文彦, 柳田敏雄

    生体運動研究合同班会議  2011.1.8 

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    Venue:大阪市  

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  • ミオシンVの歩行中におけるレバーアームの揺らぎ

    藤田恵介, 岩城光宏, 岩根敦子, 柳田敏雄

    生体運動研究合同班会議  2011.1.8 

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    Venue:大阪市  

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  • 2SQ-02 Myosin mechanical-sensing for directional motion(2SQ Developing Mechanobiology:from fundamentals to medical applications)

    Iwane Atsuko, Yanagida Toshio

    The 49th Annual Meeting of the Biophysical Society of Japan  2011  Biophysical Society of Japan

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    Venue:Himeji, Hyogo  

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  • Influence of actin mutants to processive and non-processive myosin motility International conference

    Tomotaka Komori, Hiroaki Takagi, Masatoshi Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    The 4th Mechanobiology Workshop and Biophysical Society Joint Meeting  2010.11 

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    Venue:Singapore  

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  • Conformational state of actin necessary for myosin motility and cofilin binding, revealed by analyses of G146V actin International conference

    Taro Q, P. Noguchi, Masatoshi Morimatsu, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida, Taro Q, P. Uyeda

    The 4th Mechanobiology Workshop and Biophysical Society Joint Meeting  2010.11 

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    Venue:Singapore  

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  • Multiple actin structures in the filamentous state International conference

    Masatoshi Morimatsu, Yuichi Togashi, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida

    ”, Actin, The 4th Mechanobiology Workshop and Biophysical Society Joint Meeting  2010.11 

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    Venue:Singapore  

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  • Direct visualization of secondary structures of F-actin by electron cryomicroscopy International conference

    Takashi Fujii, Atsuko H. Iwane, Toshio Yanagida, Keiichi Namba

    The 4th Mechanobiology Workshop and Biophysical Society Joint Meeting  2010.11 

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    Venue:Singapore  

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  • Effective design of Recombinant skeletal muscle actin for observing its dynamical conformational changes International conference

    Atsuko H. Iwane, Masatoshi Morimatsu, Toshio Yanagida

    The 4th Mechanobiology Workshop and Biophysical Society Joint Meeting  2010.11 

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  • Visualizing and clarifying the mechanical properties of the myosin V Brownian search with optical tweezers International conference

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    GCOE/ Structual Biology Research Center International Symposium, Protein structure and dynamics; from molecules to assembly  2010.11 

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  • The tail domain of myosin-VI ensures the directed processive movement International conference

    Keigo Ikezaki, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida

    GCOE/ Structual Biology Research Center International Symposium, Protein structure and dynamics; from molecules to assembly  2010.11 

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  • Actin dynamic polymorphism observed by single molecule FRET International conference

    Masatoshi Morimatsu, Atsuko H. Iwane, Toshio Yanagida

    The 4th Mechanobiology Workshop and Biophysical Society Joint Meeting  2010.11 

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    Venue:Singapore  

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  • Visualizing and clarifying the mechanical properties of the Myosin V Brownian search with optical tweezers International conference

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    RIKEN 1Quantitative Biology Center (QBiC) Kick-off meeting  2010.10 

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    Venue:Osaka, Suita  

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  • The regulation mechanism for myosin VI steps

    Keigo Ikezaki, Mitsuhiro Sugawa, So Nishikawa, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida

    2010.10 

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  • Influence of actin mutant to processive and non-processive myosin motility

    Tomotaka Komori, Hiroaki Takagi, Masatoshi Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    2010.10 

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  • Direct visualization of secondary structures of F-actin by electron cryomicroscopy

    Takashi Fujii, Atsuko H. Iwane, Toshio Yanagida, Keiichi Namba

    2010.10 

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  • Recombinant skeletal muscle actin for specific fluorescent labeling

    Atsuko H. Iwane, Masatoshi Morimatsu, Toshio Yanagida

    2010.10 

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  • Observation of mechanical process for myosin II by a dark-field microscopy combined with an optical trap International conference

    Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    RIKEN Quantitative Biology Center (QBiC) Kick-off meeting  2010.10 

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    Venue:Osaka, Suita  

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  • The tail domain of myosin-VI ensures the directed processive movement International conference

    Keigo Ikezaki, Mitsuhiro Sugawa, So Nishikawa, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida

    RIKEN Quantitative Biology Center (QBiC) Kick-off meeting  2010.10 

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    Venue:Osaka, Suita  

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  • Multiple actin structures in the filamentous state

    Masatoshi Morimatsu, Yuichi Togashi, Atsuko H. Iwane, Toshio Yanagida

    RIKEN Quantitative Biology Center (QBiC) Kick-off meeting  2010.10 

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    Venue:Osaka, Suita  

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  • The mechanical properties of a single myosin V motor domain during gait motion International conference

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko. H. Iwane, Toshio Yanagida

    2010.3 

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  • Brownian-search-and-catch mechanism for myosin-VI steps International conference

    Mitsuhiro Iwaki, Atsuko H. Iwane, Tetsuya Shimokawa, Roger. Cooke, Toshio Yanagida

    2010.3 

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  • Single molecule visualization of self-regulated kinesin motility International conference

    Tomonobu M, Watanabe, Toshio Yanagida, Atsuko H. Iwane

    Biophysical Society 54th Annual Meeting  2010.2  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • Brownian search-and-catch mechanism for myosin-VI steps International conference

    Mitsuhiro Iwaki, Atsuko H. Iwane, Tetsuya Shimokawa, Roger. Cooke, Toshio Yanagida

    Biophysical Society 54th Annual Meeting  2010.2  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • Shrec measurement of myosin-VI stepping motion International conference

    Keigo Ikezaki, Mitsuhiro Sugawa, So nishikawa, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 54th Annual Meeting  2010.2  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • The mechanical properties of a single myosin V motor domain during gait motion International conference

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko. H. Iwane, T. Yanagida

    Biophysical Society 54th Annual Meeting  2010.2  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • Multiple structural forms of actin in the filamentous state International conference

    Masatoshi Morimatsu, Yuiichi Togashi, So Nishikawa, Mitsuhiro Sugawa, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 54th Annual Meeting  2010.2  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • Influence of actin mutant to processive and non-processive myosin motility International conference

    Tomotaka Komori, Hiroaki Takagi, Masatoshi Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 54th Annual Meeting  2010.2  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • ブラウニアンサーチ中のミオシンVモータードメインに対する負荷が歩行運動に及ぼす影響

    藤田恵介, 岩城光宏, 岩根敦子, 柳田敏雄

    生体運動研究合同班会議  2010.1 

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    Venue:中央大学、東京都  

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  • ミオシン6のSHREC計測

    池崎圭吾, 須河光弘, 西川宗, 小森智貴, 岩根敦子, 柳田敏雄

    生体運動研究合同班会議  2010.1 

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    Venue:中央大学、東京都  

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  • Effects of actin mutation on processive and non-processive myosin motility International conference

    Tomotaka Komori, Hiroaki Takagi, Masatoshi Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    2009.12 

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  • Single molecule visualization of self-regulated kinesin motility

    Tomonobu M, Watanabe, Toshio Yanagida, Atsuko H. Iwane

    2009.12 

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  • Brownian search-and-catch mechanism for myosin-VI steps International conference

    Mitsuhiro Iwaki, Tetsuya Shimokawa, Atsuko H. Iwane, Toshio Yanagida

    2009.12 

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    Venue:Senri Life Science center (Osaka, Toyonaka)  

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  • The influence of mutated actin filament against processive myosin-V motility

    Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida

    2009.11 

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  • Direct observation of single myosin II molecule dynamics within a myofibril

    Yasunori Komori, Atsuko H. Iwane, Toshio Yanagida

    2009.11 

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  • 3D tracking of motor proteins

    Thomas Martin, So Nishikawa, Mitsuhiro Sugawa, Atsuko H. Iwane, Toshio Yanagida

    2009.11 

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  • Two distinct forms of actin in monomeric and filamentous states

    Masatoshi Morimatsu, Yuichi Togashi, So Nishikawa, Mitsuhiro Sugawa, Atsuko H. Iwane, Toshio Yanagida

    2009.11 

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  • G146V mutation in actin inhibits force generation during interaction with skeletal myosin

    Taro Noguchi, Masatoshi Morimatsu, Kohji Ito, Atsuko H. Iwane, Toshio Yanagida

    2009.11 

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  • Visualization of self-regulated kinesin motility

    Atsuko H. Iwane, Tomonobu M, Watanabe, Toshio Yanagida

    2009.11 

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  • Quantitative analysis of strain-sensor for myosin-VI

    Mitsuhiro Iwaki, Atsuko H. Iwane, Tetsuya Shimokawa, Toshio Yanagida

    2009.11 

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  • The mechanical properties of a single myosin V motor domain during gait motion

    Keisuke Fujita, Mitsuhiro Iwaki, Atsuko H. Iwane, Masatoshi Morimatsu, Toshio Yanagida

    2009.11 

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  • ナノバイオロジー、ナノケミストリー ナノ粒子を利用したモーター蛋白質の高速1分子ナノイメージングと操作

    岩城 光宏, 西川 宗, 岩根 敦子, 柳田 敏雄

    第82回日本生化学会大会  2009.10 

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  • SHREC measurement of myosin-VI stepping motion

    Keigo Ikezaki, Mitsuhiro Sugawa, So nishikawa, Atsuko H. Iwane, Toshio Yanagida

    2009.10 

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  • Development of new microscopy for determining three dimentional orientations of single molecules

    Yuji Ohmachi, Yasunori Komori, Atsuko H. Iwane, Toshio Yanagida

    2009.10 

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  • ミオシン6の高速1分子イメージング

    有本行雄, 西川宗, 須河光弘, 岩根敦子, 柳田敏雄

    生体運動研究合同班会議  2009.1 

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    Venue:東京都(東京大学)  

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  • アクチン分子内ダイナミクスの解明を目指した1分子FRET計測

    森松賢順, 西川宗, 須河光弘, 岩根敦子, 柳田敏雄

    2009.1 

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  • The effect of the Magnesium ion in the ATPase activity and motility of Myosin Va International conference

    Atsuko H. Iwane, Takako Mimuro, Masatoshi Nishikawa, Tomotaka Komori, Yuichi Togashi, Ruriko Ogawa, Toshio Yanagida

    Single Molecule Biophysics Winter Workshop 2009  2009.1 

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    Venue:Aspen Center for Physics (Aspen, CO, USA)  

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  • A Brownian search and asymmetric catch mechanism for the directional motion of myosin-VI International conference

    Mitsuhiro Iwaki, Atsuko. H. Iwane, Toshio Yanagida

    Single Molecule Biophysics Winter Workshop 2009  2009.1 

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    Venue:Aspen Center for Physics (Aspen, CO, USA)  

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  • The role of the Magnesium ion in the ATPase activity and motility of myosin Va

    Atsuko H. Iwane, Takako Mimuro, Masatoshi Nishikawa, Tomotaka Komori, Yuichi Togashi, Ruriko Ogawa, Toshio Yanagida

    2008.12 

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  • Single myosin V tracking with improved time resolution

    So Nishikawa, Mitsuhiro Sugawa, Hiroshi Ueno, Atsuko H. Iwane, Toshio Yanagida

    2008.12 

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  • Simultaneous observation of nucleotide occupancy and mechanical displacement in a processive motor myosin-V

    Tomotaka Komori, So Nishikawa, Takayuki Ariga, Atsuko H. Iwane, Toshio Yanagida

    2008.12 

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  • High-speed scanning experiment to apply a strain to myosin II before the transition to the strong binding state

    Tomohisa Ran, Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    2008.12 

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  • Direct observation of a single myosin II molecule dynamics within a myofibril

    Yasunori Komori, Atsuko H. Iwane, Toshio Yanagida

    2008.12 

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  • Site-directed single molecule FRET measurement to clarify the intra molecular dynamics of actin

    Masatoshi Morimatsu, So Nishikawa, Mitsuhiro Sugawa, Atsuko H. Iwane, Toshio Yanagida

    2008.12 

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  • Strain-dependent search and capture mechanism for directional motion of myosin-VI

    Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    2008.12 

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  • Simultaneous observation of individual mechanochemical events of myosin V during processive 36 nm steps

    Mitsuhiro Sugawa, So Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    2008.12 

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  • Site-directed single molecule FRET measurement to clarify the intramolecule dynamics of actin International conference

    Masatoshi Morimatsu, So Nishikawa, Mitsuhiro Sugawa, Atsuko H. Iwane, Toshio Yanagida

    New Approaches to Complexity of Protein Dynamics by Single Molecule Measurements: Experiments and Theories  2008.12 

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    Venue:Osaka University (Osaka, Toyonaka)  

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  • Single myosin VI tracking with improved time resolution

    Ikuo Arimoto, So Nishikawa, Mitsuhiro Sugawa, Atsuko H. Iwane, Toshio Yanagida

    2008.12 

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  • The effect of the Magnesium ion in the ATPase activity and motility of Myosin Va International conference

    Atsuko H. Iwane, Takako Mimuro, Ruriko Ogawa, Masatoshi Nishikawa, Tomotaka Komori, Yuichi Togashi, Toshio Yanagida

    2nd International Symposium on Bio-nanosystems  2008.10 

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  • Simultaneous measurement of nucleotide occupancy and mechanical displacement in processive myosin-V

    Tomotaka Komori, So Nishikawa, Takayuki Ariga, Atsuko H. Iwane, Toshio Yanagida

    2008.10 

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    Language:Japanese   Presentation type:Poster presentation  

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  • ミオシンVのATP加水分解サイクルと変位発生の同時計測

    小森智貴, 西川宗, 有賀隆行, 岩根敦子, 柳田敏雄

    分子情報生命科学シンポジウム2008  2008.9  理化学研究所

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    Language:Japanese   Presentation type:Poster presentation  

    Venue:理化学研究所(埼玉県和光市)  

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  • Strain-dependent search and capture mechanism for directional motion of myosin-VI International conference

    Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    Gordon Research Conferences:MUSCLE & MOLECULAR MOTORS  2008.6  Gordon Research

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    Language:English   Presentation type:Poster presentation  

    Venue:Colby-Sawyer College (New London, NH, USA)  

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  • Myosin-V Makes Random Two 90° Rotations during The Walking Step International conference

    Yasunori Komori, Atsuko H. Iwane, Toshio Yanagida

    Gordon Research Conferences:MUSCLE & MOLECULAR MOTORS  2008.6  Gordon Research

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    Language:English   Presentation type:Poster presentation  

    Venue:Colby-Sawyer College (New London, NH, USA)  

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  • Simultaneous observation of individual mechanochemical events of myosin V during processive 36nm steps International conference

    Mitsuhiro Sugawa, So Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    Gordon Research Conferences:MUSCLE & MOLECULAR MOTORS  2008.6  Gordon Research

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    Language:English   Presentation type:Poster presentation  

    Venue:Colby-Sawyer College (New London, NH, USA)  

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  • Relationship between Myosin Va ATPase activity and motility International conference

    Atsuko H. Iwane, Takako Mimuro, Masatoshi, Nishikawa, Tomotaka Komori, Toshio Yanagida

    Gordon Research Conferences:MUSCLE & MOLECULAR MOTORS  2008.6  Gordon Research

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    Language:English   Presentation type:Poster presentation  

    Venue:Colby-Sawyer College (New London, NH, USA)  

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  • Single molecule detection of the two-legged nano-machine Invited

    NISHIKAWA So, SUGAWA Mitsuhiro, KOMORI Tomotaka, MORIMATSU Masatoshi, IWANE Atsuko H, YANAGIDA Toshio

    第4回電気学会バイオマイクロシステム研究会  2008.2.12 

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • Simultaneous Observation of Individual Mechanochemical Events of Myosin V during Processive 36 nm Steps International conference

    Mitsuhiro Sugawa, So Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    Joint Meeting of the Biophysical Society 52nd Annual Meeting & 16th International Biophysics  2008.2 

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    Language:English   Presentation type:Poster presentation  

    Venue:Long Beach Convention & Entertainment Center (Long Beach, CA, USA)  

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  • Strain-dependent search and capture mechanism for directional motion of myosin-VI International conference

    Mitsuhiro Iwaki, Atsuko H. Iwane, Toshio Yanagida

    Joint Meeting of the Biophysical Society 52nd Annual Meeting & 16th International Biophysics  2008.2 

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    Language:English   Presentation type:Poster presentation  

    Venue:Long Beach Convention & Entertainment Center (Long Beach, CA, USA)  

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  • Mechanochemical Coupling In Actomyosin Motility With Fluctuation Analysis International conference

    Masatoshi Nishikawa, Hiroaki Takagi, Atsuko H. Iwane, T. Yanagida

    Joint Meeting of the Biophysical Society 52nd Annual Meeting & 16th International Biophysics  2008.2 

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    Language:English   Presentation type:Poster presentation  

    Venue:Long Beach Convention & Entertainment Center (Long Beach, CA, USA)  

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  • Direct observation of chemo-mechanical coupling in single headed myosin V International conference

    Tomotaka Komori, So Nishikawa, Takayuki Ariga, Atsuko. H. Iwane, Toshio Yanagida

    Joint Meeting of the Biophysical Society 52nd Annual Meeting & 16th International Biophysics  2008.2 

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    Language:English   Presentation type:Poster presentation  

    Venue:Long Beach Convention & Entertainment Center (Long Beach, CA, USA)  

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  • 単頭ミオシンVの化学-力学反応の直接計測

    小森智貴, 西川宗, 有賀隆行, 岩根敦子, 柳田敏雄

    分子情報生命科学シンポジウム2008  2008.2 

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    Language:Japanese   Presentation type:Poster presentation  

    Venue:北海道大学(北海道札幌市)  

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  • Myosin-V makes two 90゜Brownian rotation per 36nm step International conference

    Yasunori Komori, Atsuko. H. Iwane, Toshio Yanagida

    Joint Meeting of the Biophysical Society 52nd Annual Meeting & 16th International Biophysics  2008.2 

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    Language:English   Presentation type:Poster presentation  

    Venue:Long Beach Convention & Entertainment Center (Long Beach, CA, USA)  

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  • Relationship between Myosin Va ATPase activity and motility International conference

    Atsuko H. Iwane, Takako Mimuro, Masatoshi Nishikawa, Tomotaka Komori, Toshio Yanagida

    Joint Meeting of the Biophysical Society 52nd Annual Meeting & 16th International Biophysics  2008.2 

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    Language:English   Presentation type:Poster presentation  

    Venue:Long Beach Convention & Entertainment Center (Long Beach, CA, USA)  

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  • オシンVIの張力依存的な方向性制御機構

    岩城光宏, 岩根敦子, 欄 智久, 柳田敏雄

    分子情報生命科学シンポジウム2008  2008.2 

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    Language:Japanese   Presentation type:Poster presentation  

    Venue:北海道大学(北海道札幌市)  

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  • ミオシンVIの張力依存的な方向性制御機構

    岩城光宏, 岩根敦子, 柳田敏雄

    2008年生体運動研究合同班会議  2008.1 

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    Venue:仙台市戦災復興記念館 記念ホール(宮城県仙台市)  

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  • ミオシンV は2 度のブラウン回転運動により36nm ステップの歩行をする

    小森靖則, 岩根敦子, 柳田敏雄

    2008年生体運動研究合同班会議  2008.1 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台市戦災復興記念館 記念ホール(宮城県仙台市)  

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  • アクトミオシン動作メカニズム解明に向けて“遺伝子工学的手法からのアプローチ Invited

    岩根敦子

    07特定領域研究「生体ナノシステムの制御」分科会  2007.11.16 

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    Presentation type:Oral presentation (invited, special)  

    Venue:東京大学(東京都文京区)  

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  • 1分子力学計測により現れたネクチン・カドヘリンの複数結合の振舞いの違い(Difference of multiple bonds behaviour between nectin and cadherin revealed by single molecule force measurements) Invited

    塚崎 克和, 喜多村 和郎, 清水 和也, 岩根 敦子, 高井 義美, 柳田 敏雄

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2007.11 

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  • Simultaneous Observation of ATPase and Displacement by Myosin V International conference

    Tomotaka Komori, So Nishikawa, Takayuki Ariga, Atsuko H. Iwane, Toshio Yanagida

    Biocomp2007, Collective Dynamics: Topics on Competition and Cooperation in the Bioscienced  2007.9.25 

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    Language:English   Presentation type:Poster presentation  

    Venue:Hotel Lloyd's Baia(Italy, Vietri Sul Mare)  

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  • FRETを利用した新しい1分子酵素反応可視化技術

    須河光弘, 西川宗, 岩根敦子, 柳田敏雄

    特定領域研究「膜超分子モーターの革新的ナノサイエンス」 第2回班会議  2007.9 

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    Venue:良野プリンスホテル、(北海道富良野市)  

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  • Strain-Dependent Binding Rate Controls Directionality of Molecular Motor International conference

    Mitsuhiro Iwaki, Atsuko H. Iwane, Mitsuo Ikebe, Toshio Yanagida

    Biocomp2007, Collective Dynamics: Topics on Competition and Cooperation in the Bioscienced  2007.9 

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    Language:English   Presentation type:Poster presentation  

    Venue:Hotel Lloyd's Baia(Italy, Vietri Sul Mare)  

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  • Measurement System for Mechano-Chemical Coupling: Combining Optical Tweezers and Fluorescent Microscopy International conference

    Takayuki Ariga, Tomotaka Komori, So Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    Biocomp2007, Collective Dynamics: Topics on Competition and Cooperation in the Bioscienced  2007.9 

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    Language:English   Presentation type:Poster presentation  

    Venue:Hotel Lloyd's Baia(Italy, Vietri Sul Mare)  

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  • The micro needle study of the relationship between myosin and a single actin filament International conference

    Masatoshi Morimatsu, So Nishikawa, Yoshikazu Tsukasaki, Takuya Okada, Atsuko H. Iwane, Toshio Yanagida

    2007.8 

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  • How conventional kinesin regulates its tails during movement International conference

    Atsuko. H. Iwane, Tomonobu. M, Watanabe, Toshio Yanagida

    56th Fujihara Seminar The Molecular Motor Conference 2007  2007.8 

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  • Single molecule FRET imaging of enzymatic reactions at high concentrations of fluorescently labeled ligand International conference

    Mitsuhiro Sugawa, So Nisikawa, Atsuko H. Iwane, Toshio Yanagida

    56th Fujihara Seminar The Molecular Motor Conference 2007  2007.8 

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    Venue:Grand Hotel New Oji (Hokkaido, Tomakomai)  

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  • Simultaneous Observation of ATPase and Displacement by Myosin V International conference

    Tomotaka Komori, So Nishikawa, Takayuki Ariga, Atsuko H. Iwane, Toshio Yanagida

    2007.8 

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  • 単一細胞接着分子ネクチンとカドヘリンの多様な結合の役割の高感度力測定による解明(Role of multiple bonds between the single cell adhesion molecules, nectin and cadherin, revealed by high sensitive force measurements)

    塚崎 克和, 喜多村 和郎, 清水 一也, 岩根 敦子, 高井 義美, 柳田 敏雄

    日本発生生物学会・日本細胞生物学会合同大会要旨集  2007.5 

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  • 高感度1分子間結合力計測によりあらわれたネクチン・カドヘリンの複数結合の役割

    塚崎克和, 喜多村和郎, 清水和也, 岩根敦子, 高井義美, 柳田敏雄

    第40回日本発生生物学会・第59回日本細胞生物学会合同大会  2007.5 

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    Presentation type:Oral presentation (general)  

    Venue:福岡国際会議場 (福岡県福岡市)  

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  • The Construction of Measurement System for Simultaneous Observation of Myosin V International conference

    Tomotaka Komori, So Nishikawa, Takayuki Ariga, Masatoshi Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    Biophysical society Fifty and first Annual Meeting  2007.3 

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    Language:English   Presentation type:Poster presentation  

    Venue:The Baltimore convention center (Baltimore, MD, USA)  

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  • Coiled-coil Domain of Myosin V International conference

    Takayuki Ariga, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida

    Biophysical society Fifty and first Annual Meeting  2007.3  Biophysical Society

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    Language:English   Presentation type:Poster presentation  

    Venue:The Baltimore convention center (Baltimore, MD, USA)  

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  • Single Molecule FRET Imaging of Enzymatic Reactions at High Concentrations of Fluorescently Labeled Ligands International conference

    Mitsuhiro Sugawa, So Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    Biophysical society Fifty and first Annual Meeting  2007.3  Biophysical Society

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    Language:English   Presentation type:Poster presentation  

    Venue:The Baltimore convention center (Baltimore, MD, USA)  

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  • ミオシン5のATP加水分解と変位の同時計測

    小森智貴, 西川宗, 有賀隆行, 岩根敦子, 柳田敏雄

    2007年生体運動研究合同班会議  2007.1 

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    Presentation type:Oral presentation (general)  

    Venue:金沢市文化ホール(石川県金沢市)  

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  • 高濃度蛍光ATPで1分子イメージング

    須河光弘, 西川宗, 岩根敦子, 柳田敏雄

    2007年生体運動研究合同班会議  2007.1 

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    Presentation type:Oral presentation (general)  

    Venue:金沢市文化ホール(石川県金沢市)  

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  • 2P141 Fluctuation analysis of actomyosin sliding movement and mechanochemical coupling(Molecular motors,Poster Presentations)

    Nishikawa Masatoshi, Takagi Hiroaki, Iwane Atsuko H, Yanagida Toshio

    Seibutsu Butsuri  2007 

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  • 2P125 Single myosin V tracking with improved time resolution(Molecular motors,Poster Presentations)

    Nishikawa So, Sugawa Mitsuhiro, Ueno Hiroshi, Iwane Atsuko H, Yanagida Toshio

    Seibutsu Butsuri  2007 

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  • 2P123 Relationship between actin-activated ATPase activity and motility of Myosin Va.(Molecular motors,Poster Presentations)

    Iwane Atsuko H, Nishikawa Masatoshi, Komori Tomotaka, Mimuro Takako, Ariga Takayuki, Yanagida Toshio

    Seibutsu Butsuri  2007 

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  • 2P124 Direct observation of mechanochemical coupling in single-headed myosin V(Molecular motors,Oral Presentations)

    Komori Tomotaka, Nishikawa So, Ariga Takayuki, Iwane Atsuko H, Yanagida Toshio

    Seibutsu Butsuri  2007 

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  • 2P126 Simultaneous observation of individual mechanochemical events of myosin V during processive 36 nm steps(Molecular motors,Poster Presentations)

    Sugawa Mitsuhiro, Nishikawa So, Iwane Atsuko, Yanagida Toshio

    Seibutsu Butsuri  2007 

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  • 2P127 Two Brownian 90° Rotations of Myosin-V during 36 nm-Step(Molecular motors,Poster Presentations)

    Komori Yasunori, Iwane Atsuko H, Yanagida Toshio

    Seibutsu Butsuri  2007 

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  • 2P136 Strain-dependent strong binding of myosin to actin revealed by high-speed scanning experiment(Molecular motors,Oral Presentations)

    Iwaki Mitsuhiro, Iwane Atsuko H, Yanagida Toshio

    Seibutsu Butsuri  2007 

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  • ミオシンV変異体のATPase活性と運動活性の相関について

    三室孝子, 有賀隆行, 森松賢順, 岩根敦子, 柳田敏雄

    日本分子生物学会2006フォーラム  2006.12  日本分子生物学会

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    Venue:名古屋国際会議場(愛知県名古屋市)  

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  • Single-molecule physiology and single-molecule manipulation of biomolecules Introduction

    岩根敦子

    日本分子生物学会2006フォーラム  2006.12  日本分子生物学会

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    Venue:名古屋国際会議場(愛知県名古屋市)  

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  • Multiple domain interactions between the single cell adhesion molecule, nectin and cadherin, revealed by high sensitive force measurements International conference

    Yoshikazu Tsukasaki, Kazuo Kitamura, Kazuya Shimizu, Atsuko H. Iwane, Yoshimi Takai, Toshio Yanagida

    Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan  2006.11 

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    Language:English   Presentation type:Poster presentation  

    Venue:Okinawa Convention Center (Okinawa, Naha)  

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  • Analysis of AFM images of Protein in Light of its Atomic Model International conference

    Mitsuru Sakashita, Noriyuki Kodera, Hidemi Nagao, Atsuko H. Iwane, Toshio Yanagida, Toshio Ando

    Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan  2006.11 

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    Venue:Okinawa Convention Center (Okinawa, Naha)  

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  • Multiple conformational transition of protein as monitored by dielectric spectroscopy International conference

    Yoshiyuki Arai, Atsuko H. Iwane, Tetsuichi Wazawa, Hiroaki Yokota, Yoshiharu Ishi, Toru Kataoka, Toshio Yanagida

    Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan  2006.11 

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    Venue:Okinawa Convention Center (Okinawa, Naha)  

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  • The head of myosin class-V motor moves along an actin filament with regular, ~5.5 nm steps International conference

    Takuya Okada, Hiroto Tanaka, Atsuko H. Iwane, Kazuo Kitamura, Mitsuo Ikebe, Toshio Yanagida

    Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan  2006.11 

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    Language:English   Presentation type:Poster presentation  

    Venue:Okinawa Convention Center (Okinawa, Naha)  

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  • Single molecule FRET imaging of enzymatic reactions at high concentrations of fluorescently labeled ligands International conference

    Mitsuhiro Sugawa, So Nishikawa, Atsuko H. Iwane, Takako Mimuro, Toshio Yanagida

    Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan  2006.11 

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    Language:English   Presentation type:Poster presentation  

    Venue:Okinawa Convention Center (Okinawa, Naha)  

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  • Coiled-coil domain of myosin V International conference

    Takayuki Ariga, Tomotaka Komori, Atsuko H. Iwane, Toshio Yanagida

    Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan  2006.11 

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    Language:English   Presentation type:Poster presentation  

    Venue:Okinawa Convention Center (Okinawa, Naha)  

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  • The construction of measurement system for simultaneous observation of myosin V International conference

    Tomotaka Komori, So Nishikawa, Takayuki Ariga, Atsuko H. Iwane, Hisashi Yamakawa, Osamu Ohara, Toshio Yanagida

    Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan  2006.11 

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  • The micro needle study of the relationship between myosin and a single actin filament International conference

    Masatoshi Morimatsu, So Nishikawa, Yoshikazu Tsukasaki, Takuya Okada, Atsuko H. Iwane, Toshio Yanagida

    Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan  2006.11 

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    Venue:Okinawa Convention Center (Okinawa, Naha)  

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  • Self-regulated kinesin motility International conference

    Tomonobu M, Watanabe, Toshio Yanagida, Atsuko H. Iwane

    Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan  2006.11 

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    Venue:Okinawa Convention Center (Okinawa, Naha)  

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  • Single molecular visulization of self-regulated kinesin motility International conference

    Atsuko H. Iwane, Tomonobu M, Watanabe, Toshio Yanagida

    International Symposium on Bio-nanosystems  2006.9 

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    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:Hotel Matsushima Taikannsou (Miyagi, Sendai)  

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  • Biased diffusion model driven by a single-headed myosin International conference

    Mitsuhiro Iwaki, Atsuko H. Iwane, Mitsuo Ikebe, Toshio Yanagida

    Physics of Molecular Machine, Ecole de Physique des Houches  2006.5 

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    Venue:La Cete des Chavants (Les Houches, France)  

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  • Single molecular visulization of self-regulated kinesin motility International conference

    Atsuko H. Iwane, Tomonobu M, Watanabe, Toshio Yanagida

    Gordon Research Conferences:Single Molecule Approaches To Biology  2006.5  Gordon Research

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    Venue:Colby-Sawyer College (New London, NH, USA)  

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  • Further Characterization of Novel Crossbridge Conformation During Actin-Sliding International conference

    Eisaku Katayama, Yoshitaka Kimori, Yosuke Oguchi, Atsuko H. Iwane, Toshio Yanagida, Norio Baba

    Biophysical Society 50th Annual Meeting  2006.2  Biophysical Society

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    Language:English   Presentation type:Poster presentation  

    Venue:Salt Lake City Convention Center (Salt Lake City, UT, USA)  

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  • Direct Observation of molecular recognition between cell adhesion molecules using flexible glass microneedle International conference

    Yoshikazu Tsukasaki, Kazuo Kitamura, Kazuya Simizu, Atsuko H. Iwane, Yoshimi Takai, Toshio Yanagida

    Biophysical Society 50th Annual Meeting  2006.2  Biophysical Society

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    Venue:Salt Lake City Convention Center (Salt Lake City, UT, USA)  

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  • Development of a novel microscope for simultaneous observation of mechano-chemical coupling International conference

    Takayuki Ariga, Tomotaka Komori, So Nishikawa, Atsuko H. Iwane, Toshio Yanagida

    Biophysical Society 50th Annual Meeting  2006.2  Biophysical Society

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    Venue:Salt Lake City Convention Center (Salt Lake City, UT, USA)  

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  • Processivity of cargo bound single-headed wild-type myosin-VI International conference

    Mitsuhiro Iwaki, Hiroto Tanaka, Atsuko H. Iwane, Mitsuo Ikebe, Toshio Yanagida

    Biophysical Society 50th Annual Meeting  2006.2  Biophysical Society

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    Venue:Salt Lake City Convention Center (Salt Lake City, UT, USA)  

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  • Biased diffusion model driven by a single-headed myosin International conference

    Mitsuhiro Iwaki, Hiroto Tanaka, Atsuko H. Iwane, Mitsuo Ikebe, Toshio Yanagida

    Biophysical Society 50th Annual Meeting  2006.2  Biophysical Society

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    Venue:Salt Lake City Convention Center (Salt Lake City, UT, USA)  

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  • Single Myosin-V and-VI head step along the actin filament with ~ 5.5 nm steps International conference

    Takuya Okada, Kazuo Kitamura, Hiroto Tanaka, Atsuko H. Iwane, Mitsuo Ikebe, Toshio Yanagida

    Biophysical Society 50th Annual Meeting  2006.2  Biophysical Society

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    Venue:Salt Lake City Convention Center (Salt Lake City, UT, USA)  

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  • アクトミオシン滑り運動に伴うクロスブリッジの挙動

    片山栄作, 木森義隆, 小口洋介, 岩根敦子, 柳田敏雄, 馬場則男

    2006年生体運動研究合同班会議  2006.1.7 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学弥生会館(東京都文京区)  

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  • 単頭ミオシンVはアクチンフィラメント上を〜5.5nmのステップで連続的に運動する

    岡田拓也, 田中裕人, 岩根敦子, 喜多村和朗, 柳田敏雄

    2006年生体運動研究合同班会議  2006.1 

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    Venue:東京大学弥生会館(東京都文京区)  

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  • Myosin-V and –VI head step along the actin filament with ~ 5.5 nm steps.

    T. Okada, K. Kitamura, H. Tanaka, A. H. Iwane, M. Ikebe, T. Yanagida

    The 33th SEIRIKEN Conference: Troponin-Conference, Regulatory proteins of striated muscle-structure, function and disorder  2005.10.25 

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    Venue:Okazaki, Aichi, JAPAN  

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  • Cargo binding regulates the processivity of single-headed wild-type myosin-IV. International conference

    A. H. Iwane

    61st Harden Conference Molecular Motors: Structure and Function  2005.9.20 

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    Venue:Robinson College, Cambridge, UK  

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  • A single-headed Myosin-V moves along an actin filament with ~5.6 nm steps. International conference

    T. Okada, K. Kitamura, H. Tanaka, A. H. Iwane, M. Ikebe, T. Yanagida

    61st Harden Conference Molecular Motors: Structure and Function  2005.9.19 

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    Venue:Robinson College, Cambridge, UK  

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  • Cargo binding makes wild-type single-headed myosin-VI move processively. International conference

    M. Iwaki, H. Tanaka, A. H. Iwane, E. Katayama, M. Ikebe, T. Yanagida

    Gordon Reseach Conference MUSCLE:CONTRACTILE PROTEINS  2005.9.5  Gordon Research

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    Venue:Colby-Sawyer College, New London, New Hampshire, USA  

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  • Direct Obervation of Biomolecule Nano-reaction-field Using Flexible Intermolecular Force Microscopy. International conference

    Y. Tsukasaki, K. Kitamura, K. Shimizu, A. H. Iwane, Y. Takai, T. Yanagida

    The International Conference on Nanotechnology: Science and Application (Nanotech Insight 2005)  2005.2.22 

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    Venue:Luxor, Egypt  

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  • Cargo binding makes wild-type single-headed myosin-VI move processively. International conference

    M. Iwaki, H. Tanaka, A. H. Iwane, T. Yanagida, M. Ikebe

    Biophysical society 49th Annual Meeting  2005.2.16  Biophysical Society

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    Venue:Long Beach, California, USA  

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  • Loose coupling mechanism of muscle contraction based on stochastic properties of single actomyosin motors observed in vitro. International conference

    A. H. Iwane, K. Kitamura, M. Tokunaga, S. Esaki, T. Yanagida

    Single Molecule Biophysics Winter Workshop  2005.1.4 

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    Venue:Aspen, Colorado, USA  

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  • 確率的に5.5 nmステップをするミオシン頭部間の共同性が1ATP当たり60nm以上の滑走距離を生み出す

    江崎誠治, 喜多村和郎, 徳永万喜洋, 岩根敦子, 柳田敏雄

    2005年生体運動研究合同班会議プログラム  2005.1 

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    Venue:千里ライフサイエンスセンター、大阪  

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  • 単頭ミオシンVIの連続的な運動の獲得と運動メカニズム

    岩城光宏, 田中裕人, 岩根敦子, 片山栄作, 池辺光男, 柳田敏雄

    2005年生体運動研究合同班会議プログラム  2005.1 

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    Venue:千里ライフサイエンスセンター、(大阪府豊中市)  

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  • 高感度分子間力顕微鏡により検出された細胞間接着分子の新しい分子認識メカニズム

    塚崎克和, 喜多村和朗, 清水一也, 岩根敦子, 高井義美, 柳田敏雄

    2005年生体運動研究合同班会議プログラム  2005.1 

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    Venue:千里ライフサイエンスセンター、大阪  

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  • 2P194 Single molecule observation of ATP hydrolysis of Myosin V

    Komori T, Nishikawa S, Ariga T, Iwane A.H, Yamakawa H, Ohara O, Yanagida T

    Seibutsu Butsuri  2005 

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  • 2P116 Quantitative single molecule FRET measurement for structural dynamics study

    Sugawa M, Arai Y, Iwane A.H, Ishii Y, Yanagida T

    Seibutsu Butsuri  2005 

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  • 1P049 Single molecule FRET observation of conformation in mutant Ras incorporated fluorescence dye into the its SwitchII region

    ARAI Y, Wazawa T, Iwane A, Yokota H, Ishii Y, Kataoka T, Yanagida T

    Seibutsu Butsuri  2005 

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  • 1P113 Single-molecule imaging of cyanobacterial circadian clock proteins

    Wada C, Arai Y, Hayashi F, Iwase R, Uzumaki T, Iwane A.H, Yanagida T, Ishiura M

    Seibutsu Butsuri  2005 

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  • 1P180 Biased diffusional motion of cargo bound single-headed myosin-VI observed in high viscous solution

    Iwaki M, Ikebe M, Yanagida T

    Seibutsu Butsuri  2005 

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  • 2P196 Processive movement of Myosin V without swing of lever arm domain

    Komori Y, Iwane A.H, Ikebe M, Yanagida T

    Seibutsu Butsuri  2005 

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  • 2P320 Development of a novel microscope for sirmultaneous observation of mechanochemical coupling

    Ariga T, Komori T, Nishikawa S, Iwane A, Yanagida T

    Seibutsu Butsuri  2005 

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  • 3P176 A single head myosin V moves along an actin filament with 5.6 nm steps

    Okada T.okada, Kitamura K, Tanaka H, Iwane A.H, Ikebe M, Yanagida T

    Seibutsu Butsuri  2005 

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  • 3P177 Easy system to detect the step by using glass needle

    Morimatsu M, Nishikawa S, Tsukasaki Y, Okada T, Iwane H, Yanagida T

    Seibutsu Butsuri  2005 

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  • 2P117 Direct Observation of Cell adhesion molecule Nanoreaction-field Using Flexible Intermolecular Force Microscopy

    Tsukasaki Y, Kitamura K, Shimizu K, Iwane A.H, Takai Y, Yanagida T

    Seibutsu Butsuri  2005 

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  • 高感度1分子相互作用計測により検出された細胞間接着分子ダイナミクス

    塚崎克和, 喜多村和郎, 清水一也, 岩根敦子, 高井義美, 柳田敏雄

    第27回日本分子生物学会年会  2004.12  日本分子生物学会

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    Venue:神戸ポートアイランド、神戸  

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  • ミオシンVIは小胞輸送時に一方向運動能を獲得して動く

    岩城光宏, 三室孝子, 田中裕人, 池辺光男, 岩根敦子, 柳田敏雄

    第27回日本分子生物学会年会  2004.12  日本分子生物学会

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    Venue:神戸ポートアイランド、神戸  

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  • Novel stable Interactions Between Cell Adhesion Molecules Detected Using Intermolecular Force Microscopy at Sub-Piconewton Resolution. International conference

    Y. Tsukasaki, K. Kitamura, K. Shimizu, A. H. Iwane, Y. Takai, T. Yanagida

    10th International Workshop on“ Single Molecule Detection and Ultrasensitive Analysis in Life Sciences”  2004.9.23 

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    Venue:WISTA Campus, Berlin-Adlershot, Germany  

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  • How does myosin without rigid long lever arm generate large steps? International conference

    A. H. Iwane, T. Yanagida

    Molecular Motors Meeting  2004.2.28 

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    Venue:Alpbach, Austria  

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  • Asymmetric binding of myosin V and VI to an actin filament. International conference

    Y. Komori, H. Tanaka, A. H. Iwane, Y. Ishii, M. Ikebe, T. Yanagida

    Biophysical society 48th Annual Meeting  2004.2.15  Biophysical Society

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    Venue:San Antonio, Texas, USA  

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  • 3P151 The method of force measurment, after checking single molecules

    Nishikawa S, Nishikawa M, Zulliger Martin, Iwane A.H, Yanagida T

    Seibutsu Butsuri  2004 

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  • 14aPS-82 How Myosin VI May Walk

    Ali Yusuf M, Homma K, Iwane A, Adachi K, Itoh H, Kinosita K. Jr, Yanagida T, Ikebe M

    Meeting Abstracts of the Physical Society of Japan  2004 

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  • 1P030 Novel stable interactions between cell adhesion molecules detected using intermolecular force microscopy at sub-piconewton resolution

    Tsukasaki Y, kitamura K, Shimizu K, Iwane A. H, Takai Y, Yanagida T

    Seibutsu Butsuri  2004 

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  • 1P031 Development of a quantitative measurement system for single molecule FRET

    Sugawa M, Arai Y, Iwane A.H, Yanagida T

    Seibutsu Butsuri  2004 

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  • Rasの構造変化の1分子イメージング

    新井由之, 岩根敦子, 和沢鉄一, 横田浩章, 石井由晴, 片岡徹, 柳田敏雄

    第26回日本分子生物学会年会  2003.12  日本分子生物学会

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  • 情報伝達分子Ras/Raf相互作用のin vitro 1分子イメージング

    飯塚千乃, 新井由之, 渡邊朋信, 岩根敦子, 片岡徹, 柳田敏雄

    第26回日本分子生物学会年会  2003.12  日本分子生物学会

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  • 低分子量G蛋白質H-Rasの構造のダイナミクス

    岩根敦子, 佐甲靖志

    第26回日本分子生物学会年会  2003.12  日本分子生物学会

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  • 光ピンセットを用いた単頭ミオシンVの1分子ナノ計測

    渡邊朋信, 田中裕人, 岩根敦子, 眞木さおり, 池辺光男, 柳田敏雄

    第26回日本分子生物学会年会  2003.12  日本分子生物学会

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  • Load dependence of step size and kinetics of single myosin VI molecules. International conference

    M. Iwaki, H. Tanaka, A. H. Iwane, K. Homma, R. Ikebe, T. Yanagida, M. Ikebe

    Biophysical society 47th Annual Meeting  2003.3.2  Biophysical Society

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    Venue:San Antonio, Texas, USA  

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  • Single molecule nanometry of single-headed myosin-V by optical tweezer. International conference

    T. M, Watanabe, H. Tanaka, A. H. Iwane, K. Homma, R. Ikebe, M. Ikebe, T. Yanagida

    Biophysical society 47th Annual Meeting  2003.3.2  Biophysical Society

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    Venue:San Antonio, Texas, USA  

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  • Simultaneous observation of rotatory and stepping motions of single myosin VI molecules International conference

    S. Nishikawa, K. Kitamura, H. Tanaka, A. H. Iwane, K. Homma, R. Ikebe, M. Ikebe, T. Yanagida

    Biophysical society 47th Annual Meeting  2003.3.2  Biophysical Society

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    Venue:San Antonio, Texas, USA  

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  • Multiple forms of activated Ras observed directly by single molecule FRET. International conference

    Y. Arai, A.H. Iwane, T. Wazawa, H. Yokota, Y. Ishii, T. Kataoka, T. Yanagida

    Biophysical society 47th Annual Meeting  2003.3.2  Biophysical Society

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    Venue:San Antonio, Texas, USA  

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  • ミオシンVの36nmステップの中に存在するサブステップの検出

    岡田拓也, 喜多村和郎, 岩根敦子, 池辺光男, 柳田敏雄

    2003年生体運動研究合同班会議  2003.1 

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  • Introduction : Nobel Techniques for Live Cell Imaging

    Miyauchi T, Iwane A.H

    Seibutsu Butsuri  2003 

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  • Mechanical property of single myosin VI molecules which can not be explained by lever-arm hypothesis

    Iwaki M, Tanaka H, Iwane A, Ikebe M, Yanagida T

    Seibutsu Butsuri  2003 

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  • Single-molecule analysis reveals multiple binding sites of Raf1 on the plasma membrane

    Hibino K, Sako Y, Iwane A, Yanagida T

    Seibutsu Butsuri  2003 

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  • The two-headed structure of myosin-V stabilizes its processive movement.

    Watanabe T.M, Tanaka H, Iwane H, Maki-Yonekura S, Homma K, Ikebe R, Ikebe Mitsuo, Yanagida T

    Seibutsu Butsuri  2003 

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  • Multiple conformations of activated Ras observed directly by single molecule FRET

    Arai Y, Wazawa T, Yokota H, Iwane A.H, Ishii Y, Kataoka Kataoka, Yanagida T

    Seibutsu Butsuri  2003 

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  • Cell Adhesion Molecules Interaction Probed Using Intermolecular Force Microscopy

    Tsukasaki Y, Kitamura K, shimizu K, Iwane A.H, Takai Y, Yanagida T

    Seibutsu Butsuri  2003 

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  • The binding potential of actmyosin revealed by single molecule imaging

    Komori Y, Tanaka H, Iwane A.H, Ikebe M, Yanagida T

    Seibutsu Butsuri  2003 

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  • Substeps of myosin V

    Okada T, Kitamura K, Iwane A, Tanaka H, Ikebe M, Yanagida T

    Seibutsu Butsuri  2003 

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  • Rasの構造変化の1分子イメージング

    新井由之, 和沢鉄一, 横田浩章, 岩根敦子, 石井由晴, 片岡徹, 柳田敏雄

    第25回日本分子生物学会年会  2002.12  日本分子生物学会

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  • Load dependence step size of single myosin-VI molecule

    Iwaki M, Tanaka H, Iwane A. H, Homma K, Ilebe R, Yanagida T, Ikebe M

    Single Molecule Processes Project symposium  2002.11.28 

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    Venue:Senri Life science center, Osaka  

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  • How do biological motors works? International conference

    E. Katayama, T. Q, P. Uyeda, A. H. Iwane, M. Ikebe, T. Yanagida

    IV World congress biomechanics  2002.8.5 

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    Venue:Calgary, CANADA  

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  • Class VI myosin moves processively along actin filaments backwards with large steps.

    M. Iwaki, Y. Komori, S. Nishikawa, K. Homma, A. H. Iwane, J. Saito, R. Ikebe, M. Ikebe, T. Yanagida

    IV World congress biomechanics  2002.8.5 

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    Venue:Calgary, CANADA  

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  • Myosin V truncation mutant with only one IQ motif can processively move along an actin filament with 36 nm steps. International conference

    H. Tanaka, K. Homma, A. H. Iwane, E. Katayama, R. Ikebe, J. Saito, M. Ikebe, T. Yanagida

    IV World congress biomechanics  2002.8.5 

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    Venue:Calgary, CANADA  

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  • An actomyosin motor is driven by biased Brownian motion. International conference

    H. Tanaka, A. H. Iwane, M. Iwaki, Y. Komori, S. Nishikawa, T. Watanabe, E. Katayama, M. Ikebe, T. Yanagida

    Banff Symposium on Skeletal Muscle  2002.8.2 

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    Venue:The Banff Centre for the Arts Banff, Alberta, Canada  

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  • Class VI myosin are processive motors with a large step: Actin plays an essential role. International conference

    Katayama, H. Tanaka, M. Ikebe, T. Yanagida

    Gordon Research Conference 2002: Muscle: Contractile Proteins  2002.6.13  Gordon Research

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    Venue:Colby-Sawyer College, New London, New Hampshire, USA  

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  • EGF刺激によるH-Ras/cRaf-1情報伝達反応の1分子可視化解析

    日比野 佳代, 佐甲 靖志, 岩根 敦子, 柳田 敏雄

    日本細胞生物学会大会講演要旨集  2002.5 

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  • The motor domain determines the large step of myosin-V. International conference

    H. Tanaka, K. Homma, A.H. Iwane, E. Katayama, R. Ikebe, J. Saito, T. Yanagida, M. Ikebe

    Biophysical society 46th Annual Meeting  2002.2.25  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • Ras takes multiple conformations to adapt to its multiple effectors. International conference

    Y. Arai, H. Yokota, A.H. Iwane, T. Wazawa, T. Kataoka, Y. Ishii, T. Yanagida

    Biophysical society 46th Annual Meeting  2002.2.25  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • Myosin VI moves processely along actin filaments backwards with large 38nm steps. International conference

    S. Nishikawa, K. Homma, Y. Komori, M. Iwaki, A.H. Iwane, J. Saito, R. Ikebe, E. Katayama, T. Yanagida, M. Ikebe

    Biophysical society 46th Annual Meeting  2002.2.25  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • Subcellular dynamics of the localization of Ras and Raf1 visualized in living cells stinulated with EGF. International conference

    K. Hibino, Y. Sako, A.H. Iwane, T. Kataoka, T. Yanagida

    Biophysical society 46th Annual Meeting  2002.2.24  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • Actin plays an important role in the processive 36nm steps of class V and VI myosin. International conference

    E. Katayama, T.Q.P. Uyeda, A.H. Iwane, M. Ikebe, T. Yanagida

    Biophysical society 46th Annual Meeting  2002.2.23  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • Single molecule imaging of the kinesin’s self-regulated motility. International conference

    T. Watanabe, A.H. Iwane, T. Yanagida

    Biophysical society 46th Annual Meeting  2002.2.23  Biophysical Society

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    Venue:San Francisco, CA, USA  

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  • 1分子FRETによるアクチン、ミオシンの構造変化の検出

    横田浩章, 和沢鉄一, 小塚淳, 石井由晴, 柳田敏雄

    2002 年生体運動研究合同班会議  2002.1 

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  • 活性型ミオシン中間体により誘起されるアクチンフィラメント上のホットスポット

    片山栄作, 上田太郎, 岩根敦子, 池辺光男, 柳田敏雄

    2002年生体運動研究合同班会議  2002.1 

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  • 3E1430 Conformational changes of actin interacting with myosin V monitored by single molecule FRET

    Kozuka J, Yokota H, Iwane A, Ishii Y, Ikebe M, Yanagida T

    Seibutsu Butsuri  2002 

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  • 3K1345 Imaging of conformational changes in single Ras molecule by FRET

    Arai Y, Wazawa T, Yokota H, Iwane H, Ishii Y, Kataoka T, Yanagida T

    Seibutsu Butsuri  2002 

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  • 1N0900 Single-molecule imaging of H-Ras and cRaf-1 on the plasma membrane of living cells

    Hibino K, Sako Y, Iwane A. H, Yanagida T

    Seibutsu Butsuri  2002 

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  • 1E1045 Simultaneous Observation of Processive Movements and Rotatory Motions of a Single Myosin VI Molecule

    Nishikawa S, Kitamura K, Tanaka H, Iwane A. H, Ikebe M, Yanagida T

    Seibutsu Butsuri  2002 

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  • 3E1415 Conformational changes of myosin V observed by singlemolecule fluorometry

    Wazawa T, Ishii Y, Sugihara K, Iwane A, Homma K, Ikebe M, Yanagida T

    Seibutsu Butsuri  2002 

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  • 3E1400 Orientation dependent processive movement of myosin-V

    Tanaka H, Watanabe T, Homma K, Iwane A, Ikebe R, Ikebe M, Yanagida T

    Seibutsu Butsuri  2002 

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  • 3E1345 A single one-headed myosin-V moves processively with 32 nm steps

    Watanabe T, Tanaka H, Iwane A.H, Maki S, Homma K, Ikebe R, Ikebe M, Yanagida T

    Seibutsu Butsuri  2002 

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  • 3E1045 single molecule anlysis of Myosin VI measured by optical tweezers

    Iwaki M, Tanaka H, Iwane A.H, Nishikawa S, Ikebe M, Yanagida T

    Seibutsu Butsuri  2002 

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  • 3E1445 Detection of Snbsteps of Myosin V using a scanning probe

    Okada T, Kitamura K, Iwane A, Ikebe M, Yanagida T

    Seibutsu Butsuri  2002 

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  • Single-Molecule Assay of Biomolecules using GFP. International conference

    A. H. Iwane, S. Nishikawa, K. Hibino, M. Iwaki, Y. Komori, Y. Sako, T. Yanagida

    The3rd Asia-Pacific Workshop on Near-Field Optics  2001.11.28 

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    Venue:Melbourne, Australia  

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  • Single-Molecule Assay of Biomolecules using GFP. International conference

    A. H. Iwane, T. Yanagida

    Multi-dimensional Microscopy 2001  2001.11.26 

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    Venue:Melbourne, Australia  

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  • Actin plays an essential role in processive 36nm steps of class VI. International conference

    S. Nishikawa, K. Homma, Y. Komori, M. Iwaki, A. H. Iwane, J. Saito, R. Ikebe, E. Katayama, T. Yanagida, M. Ikebe

    International Workshop, Actin filaments from structure to mechanism  2001.11.16 

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    Venue:Spring-8, Hyogo, JAPAN,  

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  • Periodic binding of myosin VI or mutants myosin II along one side of actin filaments at 36nm intervals in the presence of ATP: A possible origin of myosin’s 36nm stepping motion. International conference

    E. Katayama, T. Q, P. Uyeda, A. H. Iwane, M. Ikebe, T. Yanagida

    International Workshop, Actin filaments from structure to mechanism  2001.11.16 

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    Venue:Spring-8, Hyogo, JAPAN,  

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  • Blinking and its effect on single molecule FRET measurements. International conference

    H. Yokota, Y. Arai, H. Matsuura, A. H. Iwane, Y. Ishii, T. Yanagida

    4th International Conference on Biological physics  2001.7.30 

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    Venue:Kyoto International Conference Hall, Kyoto, JAPAN,  

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  • Motility of single one-headed kinesin molecules along microtubules. International conference

    Y. Inoue, A. H. Iwane, T. Miyai, E. Muto, T. Yanagida

    4th International Conference on Biological physics  2001.7.30 

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    Venue:Kyoto International Conference Hall, Kyoto, JAPAN,  

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  • Kinesin motility is regulated by an inhibition of the attachment of the motor to microtubules by the tail. International conference

    T. Watanabe, A. H. Iwane, T. Yanagida

    4th International Conference on Biological physics  2001.7.30 

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    Venue:Kyoto International Conference Hall, Kyoto, JAPAN,  

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  • The motor domain, not a long neck domain, determines the large step of myosin V. International conference

    H. Tanaka, K. Homma, A. H. Iwane, E. Katayama, R. Ikebe, J. Saito, T. Yanagida, M. Ikebe

    4th International Conference on Biological physics  2001.7.30 

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    Venue:Kyoto International Conference Hall, Kyoto, JAPAN,  

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  • Stochastic properties of the stepwise movements produced by a single myosin head during one biochemical cycle of ATP hydrolysis. International conference

    K. Kitamura, M. Tokunaga, A. H. Iwane, T. Yanagida

    4th International Conference on Biological physics  2001.7.30 

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    Venue:Kyoto International Conference Hall, Kyoto, JAPAN,  

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  • EGF刺激によるRasからcRaf-1への情報伝達の細胞内可視化解析

    日比野 佳代, 佐甲 靖志, 岩根 敦子, 岡田 知代, 片岡 徹, 柳田 敏雄

    日本細胞生物学会大会講演要旨集  2001.5 

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  • The motor domain, not a long neck domain, determines the large step of myosin V. International conference

    H. Tanaka, K. Homma, A. H. Iwane, E. Katayama, R. Ikebe, J. Saito, T. Yanagida, M. Ikebe

    Biophysical society 45th Annual Meeting  2001.2.18  Biophysical Society

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    Venue:Boston, Massachusetts, USA  

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  • Single molecule mechanics of actomyosin motor.

    H. Tanaka, K. Kitamura, K. Homma, A. H. Iwane, E. Katayama, R. Ikebe, J. Saito, M. Ikebe, T. Yanagida

    Single Molecule Techniques in Biophysics and Drug Discovery  2001.2.3 

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  • Motility of single kinesin molecule. International conference

    A. H. Iwane, Y. Inoue, S. Azuma, T. Yanagida

    Mathematical modeling and Subcellular Biology  2001.1.31 

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    Venue:Vietri sul Mare, Naples, Italy  

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  • Detection of protein conformational fluctuation using single molecule FRET.

    H. Yokota, Y. Arai, A. H. Iwane, Y. Ishii, T. Yanagida

    Joint Symposium on single molecule processes  2001.1.29 

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  • The motor domain, not a long neck domain, is responsible for the large step and processivity of myosin V.

    Tanaka H, Homma K, Iwane A.H, Katayama E, Ikebe R, Saito J, Yanagida T, Ikebe M

    Seibutsu Butsuri  2001 

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  • Imaging of Single MyosinV and VI Moving along Actin Filaments

    Nishikawa S, Yanagida T, Homma K, Komori Y, Iwaki M, Tanaka H, Kitamura K, Iwane A, Ikebe R, Ikebe M

    Seibutsu Butsuri  2001 

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  • Single molecule nanometry of Myosin VI by optical tweezers : Single Myosin VI molecule moves processively along an actin filament

    Iwaki M, Nishikawa S, Komori Y, Tanaka H, Kitamura K, Iwane A, Ikebe R, Ikebe M, Yanagida T

    Seibutsu Butsuri  2001 

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  • The regulation of the Kinesin's tail domain by single molecule imaging

    WATANABE Tomonobu, IWANE Atsuko H, YANAGIDA Toshio

    Seibutsu Butsuri  2001 

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  • Motion of oneheaded kinesin molecules without neck-linker along a microtubule

    Inoue Y, Azuma S, Iwane A.H, Yanagida T

    Seibutsu Butsuri  2001 

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  • Single molecule processes of Ras

    Arai Y, Yokota H, Iwane A.H, Wazawa T, Kataoka T, Ishii Y, Yanagida T

    Seibutsu Butsuri  2001 

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  • Signal transduction from Ras to cRaf-1 in EGF-stimulated cells

    Hibino K, Sako Y, Iwane A.H, Kataoka T, Yanagida T

    Seibutsu Butsuri  2001 

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  • Conformational changes of myosin V observed by single-molecule fluorometry

    Wazawa T, Ishii Y, Sugihara K, Iwane A, Homma K, Ikebe M, Yanagida T

    Seibutsu Butsuri  2001 

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  • Stochastic properties of the stepping motion produced by a single myosin head

    Kitamura K, Tokunaga M, Iwane A.H, Yanagida T

    Seibutsu Butsuri  2001 

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  • 2量体形成を生物蛍光タンパク質間の蛍光エネルギー移動法で観る

    岩根敦子, 和沢鉄一, 石井由晴, 井上純一郎, 柳田敏雄

    第23回日本分子生物学会年会  2000.12  日本分子生物学会

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  • テールドメインによるキネシンの運動制御過程を観る

    渡邊朋信, 岩根敦子, 柳田敏雄

    第23回日本分子生物学会年会  2000.12  日本分子生物学会

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  • 1分子イメージングによる低分子量Gタンパク質Rasの挙動解析

    新井由之, 松浦秀幸, 岩根敦子, 横田浩章, 石井由晴, 片岡徹, 柳田敏雄

    第23回日本分子生物学会年会  2000.12  日本分子生物学会

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  • The motor domain, not a long neck domain, determines the large step of myosinV. International conference

    H. Tanaka, K. Homma, A. H. Iwane, E. Katayama, R. Ikebe, J. Saito, T. Yanagida, M. Ikebe

    The 11th Takeda Science Foundation Symposium on Bioscience= COE International Conference=  2000.11.18 

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    Venue:Awaji Yumebutai International Conference Center, Hyogo, JAPAN  

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  • 上皮成長因子に応答したcRaf-1キナーゼの細胞内局在変化の可視化解析

    日比野 佳代, 佐甲 靖志, 岩根 敦子, 岡田 知代, 片岡 徹, 柳田 敏雄

    日本細胞生物学会大会講演要旨集  2000.8 

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  • A Single Myosin Head Moves along an Actin Filament with Regular Steps during One Biochemical Cycle of ATP Hydrolysis

    KITAMURA Kazuo, IWANE Atsuko H, TOKUNAGA Makio

    Seibutsu Butsuri  2000.3.25 

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    To resolve the individual mechanical events of force generation by actomyosin, we have developed a new technique to capture and directly manipulate individual myosin subfragment-1 (S1) molecules using a scanning probe. The unitary mechanical step during sliding along an actin filament is 5.3nm, but 2 to 5 rapid steps in succession often end up with total displacements of &sim;11 to &sim;30nm. Multiple steps are produced by single heads during the hydrolysis of a single ATP molecule.

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  • A single myoin head moves along an actin filament with regular ~5nm steps under loaded condition. International conference

    T. Yanagida, K. Kitamura, A. H. Iwane

    Biophysical society 44th Annual Meeting  2000.2.14  Biophysical Society

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    Venue:New Orleans, Louisiana, USA  

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  • Role of the myosin neck region in generation of movement. International conference

    T. Yanagida, H. Tanaka, A. H. Iwane, T. Okumura, S. Morimoto, T. Kusumoto, A. Ishijima

    Biophysical society 44th Annual Meeting  2000.2.14  Biophysical Society

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    Venue:New Orleans, Louisiana, USA  

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  • Motion of a single molecule of catalytic core domain of kinesin along a microtubule in the presence of ADP. International conference

    Y. Inoue, T. Miyai, A. H. Iwane, T. Yanagida

    Biophysical society 44th Annual Meeting  2000.2.13  Biophysical Society

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    Venue:New Orleans, Louisiana, USA  

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  • Myosin neck region does not act as a lever arm but rather regulates the kinetics of actin-myosin inter action. International conference

    T. Yanagida, H. Tanaka, K. Kitamura, A. H. Iwane

    Biophysical society 44th Annual Meeting  2000.2.12  Biophysical Society

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    Venue:New Orleans, Louisiana, USA  

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  • Single molecule techniques in biophysics

    Yanagida T, Tanaka H, Kitamura K, Wazawa T, Nishiyama M, Esaki S, Sako Y, Ide T, Iwane AH, Ishii Y

    The proceedings of the 9th international conference on Na/K Pump and related pumps  2000 

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  • Single molecule imaging of small G protein Ras

    Matsuura H, Arai Y, Yokota H, Iwane A, Ishii Y, Kataoka T, Yanagida T

    Seibutsu Butsuri  2000 

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  • Spatiotemporal dynamics of Ras/cRaf-1 interaction in the plasma membrane of living cells

    Hibino K, Sako Y, Iwane A.H, Okada T, Kataoka T, Yanagida T

    Seibutsu Butsuri  2000 

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  • Visualization of dimerization using fluorescence resonance energy transfer (FRET) imaging between biofluorescent proteins

    Iwane A.H, Wazawa T, Ishii Y, Inoue J, Yanagida T

    Seibutsu Butsuri  2000 

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  • The step size, processivity, and velocity of myosin V are independent on its neck length.

    Tanaka H, Honma K, Iwane A.H, Katayama E, Ikebe M, Yanagida T

    Seibutsu Butsuri  2000 

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  • Laser trapping nanometry with DIG DNA probe.

    Kimura Y, Iwane A, Tanaka H, Okamoto K, Ishijima A

    Seibutsu Butsuri  2000 

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  • Single molecule analysis of actomyosin motor : Load dependence

    Kitamura K, Hikikoshi-Iwane A, Yanagida T

    Seibutsu Butsuri  2000 

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  • Direct inhibition of kinesin motility by local anesthetics. International conference

    Y. Miyamoto, T. Mashimo, A. H. Iwane, I. Yoshiya, T. Yanagida

    2nd International workshop on anesthetic mechanisms  1999.12.9 

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    Venue:Takamatsu, JAPAN  

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  • EGF刺激によるRasからcRaf-1への情報伝達の細胞内可視化解析

    日比野加代, 佐甲靖志, 岩根敦子, 岡田智代, 片岡徹, 柳田敏雄

    第54回日本細胞生物学会大会  1999.11 

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  • タンパクの2量体化を藍色蛍光タンパクCFPと黄色蛍光タンパクYFPの間の蛍光エネルギー移動法で観る

    岩根敦子, 石井由晴, 和沢鉄一, 井上純一郎, 柳田敏雄

    第22回日本分子生物学会年会  1999.11 

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  • 単頭キネシン1分子の動きをみる

    井上裕一, 宮井孝之, 岩根敦子, 柳田敏雄

    第22回日本分子生物学会年会  1999.11 

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  • Step size per ATP molecule hydrolysis of Cysteine-free and Ctsteine-remained kinesin. International conference

    A. H. Iwane, S. Iwatani, H. Higuchi, Y. Ishii, T. Yanagida

    The 7th JST International Symposium Molecular Processes and Biosystems  1999.2.24  JST

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    Venue:Tokyo, JAPAN  

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  • A single myosin head along an actin filament with regular steps of 5.3nm. International conference

    K. Kitamura, M. Tokunaga, A. H. Iwane, T. Yanagida

    The 7th JST International Symposium Molecular Processes and Biosystems  1999.2.24  JST

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    Venue:Tokyo, JAPAN  

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  • Single-molecule mechanics of mutants myosin measured by an optical tweezers.

    H. Tanaka, A. H. Iwane, T. Okumura, T. Kusumoto, T. Yanagida

    The 7th JST International Symposium Molecular Processes and Biosystems  1999.2.24  JST

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    Venue:Tokyo, JAPAN  

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  • irect inhibition of kinesin motility by local anesthetics.

    Y. Miyamoto, T. Mashimo, A. H. Iwane, I. Yoshiya, T. Yanagida

    The 7th JST International Symposium Molecular Processes and Biosystems  1999.2.24  JST

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    Venue:Tokyo, JAPAN  

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  • Single molecule imaging of one-headed kinesin molecules interacting with a microtubule. International conference

    Y. Inoue, T. Miyai, A. H. Iwane, T. Yanagida

    The 7th JST International Symposium Molecular Processes and Biosystems  1999.2.24  JST

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    Venue:Tokyo, JAPAN  

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  • S1 attached to a glass surface through a flexible random chain can move actin as fast as intact myosin. International conference

    M. Tokunaga, A. H. Iwane, K. Kitamura, T. Yanagida

    Biophysical society 43th Annual Meeting  1999.2.14  Biophysical Society

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    Venue:Baltimore, MD, USA  

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  • A single myosin head moves along an actin filament with regular steps of ~5.5nm. International conference

    K. Kitamura, M. Tokunaga, A. H. Iwane, T. Yanagida

    Biophysical society 43th Annual Meeting  1999.2.14  Biophysical Society

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    Venue:Baltimore, MD, USA  

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  • Single molecule imaging of one-headed kinesin molecules interacting with a microtubule. International conference

    Y. Inoue, A. H. Iwane, T. Yanagida

    Biophysical society 43th Annual Meeting  1999.2.14  Biophysical Society

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    Venue:Baltimore, MD, USA  

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  • Characterization of Cysteine-free and Cysteine-remained kinesin. International conference

    A. H. Iwane, S. Iwatani, H. Higuchi, K. Shima, Y. Ishii, T. Yanagida

    Biophysical society 43th Annual Meeting  1999.2.14  Biophysical Society

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    Venue:Baltimore, MD, USA  

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  • A fuorescent spectroscopic study of ficrotubule dynamics.

    Muto E, Saito Y, Iwane A.H.K, Ishii Y

    Seibutsu Butsuri  1999 

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  • Ras mediated recruitment of Raf-1 to plasma membrane visualized in living cells.

    Hibino K, Sako Y, Iwane A, Kataoka T, Yanagida T

    Seibutsu Butsuri  1999 

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  • Single-molecule mechanics of actomyosin measured by an optical tweezers ; Role of the neck region.

    Tanaka H, Iwane A, Okumura T, Morimoto S, Kusumoto T, Ishijima A, Yanagida T

    Seibutsu Butsuri  1999 

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  • Characterization of cysteine-free and cysteine-remained kinesins

    HIKIKOSHI IWANE Atsuko, IWATANI Shintaro, HIGUCHI Hideo, ISHII Yoshiharu, YANAGIDA Toshio

    日本分子生物学会年会プログラム・講演要旨集  1998.12.1 

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  • Single molecule imaging of one-headed kinesin molecules interacting with a microtubule.

    Inoue Y, Iwane A.H, Yanagida T

    Biophysics  1998.9.7 

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  • Single-molecule mechanics of mutant myosin measured by an optical tweezers

    Tanaka H, Iwane A, Kusumoto T, Yanagida T

    Biophysics  1998.9.7 

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  • Dfrect observation of the stepping motion of a single myosin head.

    Kitamura K, Tokunaga M, Iwane A, Yanagida T

    Biophysics  1998.9.7 

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  • Laser trapping nanometry and imaging of single kinesin molecules moving along microtubules.

    Nonoyama Y, Inoue Y, Nishiyama M, Miyai T, Shima K, Iwane A, Yanagida T

    Biophysics  1998.9.7 

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  • Motility of kinesin-ncd fusion proteins.

    Miyai T, Inoue Y, Hibino K, Iwane A.H, Yanagida T

    Biophysics  1998.9.7 

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  • Myosin Subfragment-1, attached to a glass surface through a flexible chain, can move actin as fast as native myosin.

    Iwane A.H, Kitamura K, Yanagida T, Tokunaga M

    Biophysics  1998.9.7 

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  • Characterization of Cysteine-free and Cysteine-remained kinesin

    Shima K, Iwatani S, Iwane H, Inoue Y, Higuchi H, Ishii Y, Yanagida T

    Biophysics  1998.9.7 

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  • Single molecule imaging of one-headed kinesin molecules interacting with a microtubule International conference

    Y. Inoue, A. H. Iwane, T. Yanagida

    COE International Conference “ Molecular Mechanism of Intracellular Transports: The Roles of Kinesin and Dynein Superfamily Proteins”  1998.8.20  COE

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    Venue:Hayama, Kanagawa, JAPAN  

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  • Characterization of Cystein-free and Cystein-remained kinesin. International conference

    A, H. Iwane, S. Iwatani, H. Higuchi, K. Shima, Y. Ishii, T. Yanagida

    COE International Conference “ Molecular Mechanism of Intracellular Transports: The Roles of Kinesin and Dynein Superfamily Proteins”  1998.8.20  COE

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    Venue:Hayama, Kanagawa, JAPAN  

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  • 微小管モーター蛋白質キネシンに対する局所麻酔薬の直接阻害作用II

    宮本善一, 真下節, 岩根敦子, 吉矢生人, 柳田敏雄

    第16回麻酔メカニズム研究会  1998.7 

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  • 骨格筋ミオシンS1-GFPキメラ蛋白の試験管内発現と1分子機能アッセイ

    岩根 敦子

    第8回日本サイトメトリー学会  1998.6 

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  • Myosin step size determined by directly manipulating a single S1 molecule with a scanning probe. International conference

    K. Kitamura, M. Tokunaga, A. H. Iwane, T. Yanagida

    Biophysical society 42th Annual Meeting  1998.2.24  Biophysical Society

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    Venue:Kansas City, Missouri, USA  

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  • Single molecule imaging of one-headed kinesin molecules bound to a bead. International conference

    Y, Inoue, H. Higuchi, A.H. Iwane, T. Yanagida

    Biophysical society 42th Annual Meeting  1998.2.22  Biophysical Society

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    Venue:Kansas City, Missouri, USA  

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  • Single molecular assay of the individual ATP turnovers by a myosin-GFP fusion protein expression in vitro. International conference

    A, H. Iwane, T. Funatsu, Y. Harada, M. Tokunaga, O. Ohara, T. Yanagida

    Biophysical society 42th Annual Meeting  1998.2.22  Biophysical Society

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    Venue:Kansas City, Missouri, USA  

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  • 微小管モーター蛋白質キネシンに対する局所麻酔薬の直接阻害作用 in vitro motility assay及び1分子イメージング法を用いて

    宮本 善一, 真下 節, 津田 祐里, 岩根 敦子, 吉矢 生人, 柳田 敏雄

    麻酔と蘇生  1997.12 

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  • Movements of truncated motor domains of kinesin

    INOUE Y, MIYAI T, IWANE A. H, HIGUCHI H, YANAGIDA T

    Biophysics  1997.10 

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  • Preparation of cysteine-lesskinesin

    IWATANI S, IWANE A, SHIMA K, HIGUCHI H, YANAGIDA T

    Biophysics  1997.10 

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  • Expression of dictyostelium discoideum myosin head and chiken skeletal muscle myosinfusion protein

    MORIMOTO S, OKUMURA T, TANAKA H, ISHIJIMA A, IWANE A, YANAGIDA T

    Biophysics  1997.10 

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  • Direct effect of local anesthetics on kinesin-microtubule motility investigated by single-molecule imaging

    MIYAMOTO Y, MASHIMO T, H. IWANE A, YOSHIYA I, YANAGIDA T

    Biophysics  1997.10 

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  • Laser trapping nanometry for single kinesin molecules

    INOUE Y, HIGUCHI H, NISHIYAMA M, IWANE A. H, IWATANI S, MIYAI T, YANAGIDA T

    Biophysics  1997.10 

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  • Cooperative binding of kinesin molecules to a microtubule in the presence of ATP

    MUTO E, MIYAMOTO Y, FUNATSU T, HARADA Y, IWANE A, YANAGIDA T

    Biophysics  1997.10 

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  • Fluctuation of myosin conformation detected by single fluorophore polarization measurement

    SAITO K, TOKUNAGA M, H. IWANE A, YANAGIDA T

    Biophysics  1997.10 

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    Language:Japanese  

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  • Individual ATPase assay and in vitro expression of myosin-GFP fused protein

    H. IWANE A, FUNATSU T, HARADA Y, OHARA O, YANAGIDA T

    Biophysics  1997.10 

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    Language:Japanese  

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  • Single molecule manipulation and nanometry of myosin subfagment-I

    KITAMURA K, TOKUNAGA M, IWANE A, YANAGIDA T

    Biophysics  1997.10 

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  • Development of the system for detecting steps by single molecules of one-headed kinesin

    INOUE Y, HIGUCHI H, IWANE A. H, YANAGIDA T

    Biophysics  1997.10 

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    Language:Japanese  

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  • Objective-type total internal reflection fluorescence microscopy and single molecule imaging of ATPase reactions

    TOKUNAGA M, KITAMURA K, SAITO K, H. IWANE A, YANAGIDA T

    Biophysics  1997.10 

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  • ミオシンS1-GFPキメラ蛋白質の試験管内発現と一分子アッセイ

    岩根 敦子

    日本生物物理学会年会講演予稿集  1996.10 

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  • ニワトリ大人型骨格筋ミオシンS1のクローニングと発現

    森本 さゆり, 岩根 敦子, 柳田 敏雄

    日本分子生物学会年会プログラム・講演要旨集  1996.8.1 

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  • A single one-headed myosin-VI with a cargo bound processively moves with large (20-42nm) steps. International conference

    M. Iwaki, H. Tanaka, A. H. Iwane, M. Ikebe, T. Yanagida

    Single Molecule Biophysics Winter Workshop 

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    Language:English   Presentation type:Oral presentation (general)  

    Venue:Aspen Center for Physics, Aspen, Colorado, USA  

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  • アクトミオシン複合体の構造ダイナミックス解明にむけて Invited

    岩根敦子, 加藤貴之, 森松賢順, 西川宗, 難波啓一, 柳田敏雄

    特定領域研究「生体超分子構造」第4回公開シンポジウム 

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    Presentation type:Oral presentation (invited, special)  

    Venue:千里ライフサイエンスセンター、(大阪府豊中市)  

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Research Projects

  • Peroxisome-mediated trimming of outer segment in the retina for photoreceptor

    Grant number:24K02239  2024.04 - 2027.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    宮本 達雄, 木村 和博, 清木 誠, 岩根 敦子, 板橋 岳志, 弘澤 萌, 森田 知佳

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    Grant amount:\18590000 ( Direct expense: \14300000 、 Indirect expense:\4290000 )

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  • 骨形成の足場であるコラーゲン結晶体を輸送する新規メカニズム

    Grant number:21K06200  2021.04 - 2025.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    黒田 純平, 岩根 敦子

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    研究代表者は、本研究において生きた個体の中のアクチノトリキアを任意のタイミングで蛍光標識することができる蛍光プローブを発見し、ヒレ成長過程でアクチノトリキアの動態変化を追跡する新しいイメージングツールを開発することに成功した。この技術革新により、これまでに知られていなかった、アクチノトリキアの「分解」様式を発見し、この分解過程は破骨細胞によって引き起こされることも発見した。これらの成果を論文としてまとめ、海外学術雑誌に投稿・査読中となっている。また、この蛍光プローブを使ったパルス・チェイスイメージングによって、ヒレの再生過程におけるアクチノトリキアの動態を明らかにすることにも成功している。再生過程では、骨の形成に先立って、まず微小なアクチノトリキアが間充織間に均一に産生され、ゆるやかに近位―遠位方向に沿って配向化する。その後、この配向化されたアクチノトリキアは、骨間(inter-ray)で迅速に消失し、骨の先端部に残存するアクチノトリキアのみが、再生中のヒレ組織の成長に伴い、先端方向へ移動することを発見した。さらに再生過程においては、多核化した巨大な破骨細胞がヒレ先端部に出現し、これにより再生初期において、アクチノトリキアのリモデリングが速やかに引き起こされることを見出した。これらの成果について、すでに複数の国内学会にて発表しており、アクチノトリキアの分解過程に注目した研究の続報として、更なる論文の執筆が進行中である。

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  • Morphological characteristics of the genome stress-induced chromosomal tether structure

    Grant number:20K21845  2020.07 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)  Grant-in-Aid for Challenging Research (Exploratory)

    宮本 達雄, 阿久津 シルビア夏子, 岩根 敦子, 山本 卓, 松浦 伸也

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    Grant amount:\6500000 ( Direct expense: \5000000 、 Indirect expense:\1500000 )

    遺伝情報を正確に娘細胞に伝達するためには、S期のゲノム複製とそれに続く分裂期での姉妹染色分体の均等分配が必須である。ゲノムDNAの大部分はS期で複製を完了するが、高度な繰り返し配列を含むセントロメア領域、テロメア領域や脆弱部位(fragile site)はG2/M期においても複製が継続されている。複製直後のこれらの染色体領域は、高度にDNAが絡まり合った状態(catenated)であり、正確な染色体分配には、DNAの“絡み”が解消(decatenation)される必要がある。正常細胞では、Catenated DNAはDNAヘリカーゼ活性をもつ分子(BLMやPICH)によってコートされ、ultra-fine bridge (UFB)構造が形成される。分裂期が進むと、Ⅱ型トポイソメラーゼがUFB構造に取り込まれて、DNAの“絡み”が解消されるとUFB構造も消失することで、正常な染色体分配が完了する。
    我々は最近、電離放射線照射後の分裂期細胞のセントロメア間にUFB構造が発達することを見出した。しかし、UFB構造は極めて動的な構造であり、従来の電子顕微鏡による観察が困難であったために、その超微形態構造は不明のままである。また、UFB構造は、DAPIなど既知のDNA染色剤では検出できないため、通常のクロマチン構造とは異質な形態特性をもつことが示唆される。本研究では、これらの分裂期UFB構造の超微形態構造の形態特性を明らかにすることを研究目的としている。
    2021年度には、ヒト網膜色素上皮細胞株であるhTERT-RPE1細胞の集束イオンビーム-走査電子顕微鏡(FIB-SEM)の観察プロトコルの改良を行い、安定した3次元微細構造の観察が可能になった。また、1本鎖DNAをドナーとした蛍光タンパク質遺伝子のゲノム編集技術を用いたノックイン技術の整備を行った。

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  • 光合成を基盤とした細胞内共生進化の初期における宿主細胞の進化過程の解明

    Grant number:20H00477  2020.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    宮城島 進也, 小林 優介, 岩根 敦子

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    Grant amount:\44460000 ( Direct expense: \34200000 、 Indirect expense:\10260000 )

    国立環境研究所のカルチャーコレクションより取り寄せたミドリゾウリムシから共生クロレラのクローン培養株を樹立した。本クローンを再度ゾウリムシ宿主に共生させ今後の研究用に利用する培養系を確立した。同様に神戸大学より取り寄せたミドリアメーバから共生クロレラ2種のクローン培養株をそれぞれ樹立した。そのうち1種(B株)を宿主アメーバに再共生させたところ宿主アメーバに長期に維持されるが、もう1種(A株)は宿主アメーバに短期間しか維持されないことが判明した。上記のようにして準備した培養系を用い、クロレラの単独培養とそれぞれゾウリムシおよびアメーバ宿主との共生状態について共生クロレラのトランスクリプトーム情報を取得することに成功した。
    上記と並行して、今後の藻食アメーバに関する研究のために、屋外より海水性の珪藻食アメーバを単離し、培養株を確立することに成功した。
    また、盗葉緑体性鞭毛虫については、以前より維持している長期の盗葉緑体現象培養系を用いて、鞭毛虫に取り込まれるクリプト藻の経時変化に関する論文の改定を行い、研究内容の受理・発表に至った。さらに短期の盗葉緑体現象培養系(捕食に近い)の研究用のクリプト藻株と盗葉緑体性鞭毛虫との二員培養系の樹立に成功した。

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  • 細胞外基質ナノ構造の生物学的構築原理の解明

    Grant number:20K20460  2020.04 - 2023.03

    日本学術振興会  科学研究費助成事業  挑戦的研究(開拓)

    林 茂生, 岩根 敦子

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    Grant amount:\26000000 ( Direct expense: \20000000 、 Indirect expense:\6000000 )

    構造色、超撥水性などに代表される生物学的な機能的素材の形成機構を理解する事を目的として、昆虫(ショウジョウバエ)の嗅覚器官を覆うクチクラにおけるナノレベルの形態形成を研究した。嗅覚器官に見られる内径30nmの穴(ナノポア)は数nmの匂い分子の通過は可能だが大きなほこりやウイルスなどは排除する。ナノポアはクチクラの強靱性と内部環境を保護しつつ昆虫の超高感度な嗅覚受容を可能にする機能的構造である。我々が報告した小胞体分子Gore-texは嗅覚毛の細胞に特異的に発現し、その変異体ではクチクラ形成は正常なものの、ナノポアが失われ嗅覚の低下が見られる (Ando et al., Curr. Biol. 2019). 本研究ではキイロショウジョウバエを材料にナノポアの構築機構の遺伝的、発生学的および分子生物学的な理解を深める事を目的とする。2021年度はイオンビーム連続切削型電子顕微鏡(FIB-SEM)を用いた三次元的細胞内構造解析を用いて正常型個体とgore-tex変異体においてナノポアの発生過程を観察する事でgore-tex変異体におけるナノポア喪失がどのようなプロセスを経て起きるのかを追求した。構築した嗅覚毛の3次元構造からは細胞膜状にエンドサイトーシス中間体に類似した多数の膜陥入構造が確認され、細胞内膜小構造を介したて小胞体ネットワークにつながる不連続な膜経路を構成することが明らかとなった。また細胞内膜系を制御する分子を系統的に阻害する実験を行った所、細胞内膜系の正常なダイナミクスがナノポア形成に必須な事が明らかとなった。

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  • 細胞外基質ナノ構造の生物学的構築原理の解明

    Grant number:19H05548  2019.06 - 2023.03

    日本学術振興会  科学研究費助成事業 挑戦的研究(開拓)  挑戦的研究(開拓)

    林 茂生, 岩根 敦子

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    Grant amount:\26000000 ( Direct expense: \20000000 、 Indirect expense:\6000000 )

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  • How do cells assemble materials to build the body?

    Grant number:19H00994  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    Kondo Shigeru

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    Grant amount:\43680000 ( Direct expense: \33600000 、 Indirect expense:\10080000 )

    During the morphogenetic process of zebrafish fins and kawakaimen, cells assemble linear needle-like structures to form scaffolds. The goal is to understand how cells perform this orderly process. In fish fins, Kondo's team identified the cells that manufacture, move, and align actinotrichia and successfully reproduced some of the processes in vitro. They also showed that the planar alignment of actinotrichia is the force that propels the fins along the dorsoventral axis. Funayama's team partially succeeded in developing a cell type-selective gene transfer method to obtain a method to analyze the behavior of bone fragment cells. They also partially elucidated the role of bone fragments and cells in the formation of blasts.

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  • live-cell hybrid imaging of morphological dynamics of the plasma membrane

    Grant number:18KK0196  2018.10 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

    YOSHIMURA Shigehiro

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    Grant amount:\17940000 ( Direct expense: \13800000 、 Indirect expense:\4140000 )

    We established a hybrid live-cell imaging system by combining high-speed atomic force microscopy (HS-AFM) and fluorescence microscopy and revealed the detailed morphological changes in the plasma membrane and protein localizations during the clathrin-mediated endocytosis. CIP4, which has membrane-deforming activity, is recruited to the plasma membrane via small G protein Cdc42, and promotes the assembly of actin, which induces a small membrane bulge near the clathrin pit. We also established a hybrid microscope system by combining HS-AFM and highly inclined and laminated optical sheet (HILO) illumination to overcome the limitation of light diffraction, and successfully visualized a single molecule in the plasma membrane by super-resolution radial fluctuation.

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  • The role of nuclear actin filaments in cancer cells

    Grant number:18K06231  2018.04 - 2021.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    川内 敬子, 岩根 敦子

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    がん細胞が成育する基質の硬さは、抗がん剤感受性に影響を及ぼす。これまでの研究で、生育している基質が柔らかい方が、癌抑制遺伝子産物p53の活性は低くなるために抗がん剤感受性が低下することを、ヒト乳がんMCF細胞を用いて証明してきた。さらにこの際に、核内で見られる新規アクチン線維(Fアクチン)構造:核アクチンファイバーの形成が誘導されることを見出してきた。核アクチンファイバーの機能は新規構造であるが故に不明である。本研究では、核アクチンファイバーの機能および形成分子機構を解明し、それを標的として化学療法の効果を高める新たな治療法への応用を目的とした。
    はじめに、p53の活性の低下が、抗がん剤処理で誘導される核アクチンファイバーの形成に重要であるかを解析した。まず、硬い基質上で培養したMCF細胞における核アクチン線維構造に対するp53ノックダウンの影響を調べたところ、p53ノックダウンすることで抗がん剤処理により核アクチンファイバーの形成が誘導されることが示された。また、p53野生型およびp53ノックアウトマウス由来胎児線維芽細胞を用いた同様の解析でも、p53-/-MEFsでのみ核アクチンファイバーの形成が誘導された。したがって、p53は抗がん剤処理で誘導される核アクチンファイバーの形成を阻害していることが示された。次に、核アクチンファイバーの形成に関与する分子の同定をアクチン制御タンパク質に対する様々な阻害剤やshRNAを用いて試みた。その結果、アクチン重合分子FMN2が核アクチンファイバーの形成には必要であることを明らかにした。

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  • Three dimensional ultrastructural background of mitochondrial dynamics using novel CLEM method

    Grant number:15K08165  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Ohta Keisuke, Nakamura Kei-ichiro, Iwane Atsuko, Togo Akinobu, Okayama Satoko, Miyazono Yoshihiro

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    Grant amount:\4940000 ( Direct expense: \3800000 、 Indirect expense:\1140000 )

    Mitochondria shape is drastically changed by cellular physiology. Mitochondrial dysfunction such as loss of membrane potential induces fragmentation of them, but the meaning of the mitochondrial structural variation has not been established. In this study, we developed a novel observation technique to address the detail structural backbone of mitochodrial dynamics and visualized the 3D microstructure of a moment of mitocondrial structural transformation. Here, we found that uncoupler administrated dysfunctional mitochondria shrank from their tubular shape into a globular shape without any fission and fusion and most of the globular mitochondria had some dents which shape was also referred as stomatocyte shape. This result strongly suggests existanse of hypothetic mitochondrial structural support mechanisms and loss of membrane potential probably trigger dissociation of the mechanisms. So our finding would be a key to understand the unique mitochondrial shape and their dynamics.

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  • 細胞分裂過程とリンクした標的分子の網羅的マッピング方の開発

    2014 - 2017

    文部科学省  科学研究費補助金(基盤研究(B)) 

    岩根 敦子

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    Authorship:Principal investigator  Grant type:Competitive

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  • 真核生物の基本的仕組みを解明するための新しい細胞場構造技術の開発

    2014 - 2015

    文部科学省  科学研究費補助金(挑戦的萌芽研究) 

    岩根 敦子

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    Authorship:Principal investigator  Grant type:Competitive

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  • 超顕微鏡技術を用いた分化多能性細胞から終分化細胞へのアクチン繊維分化系統図の作成

    2011 - 2013

    文部科学省  科学研究費補助金(基盤研究(B)) 

    岩根 敦子

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  • 1分子イメージングを用いた揮発性麻酔薬による幼若脳神経細胞障害作用の分子機構解明

    2008 - 2012

    文部科学省  科学研究費補助金(基盤研究(C)) 

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    Grant type:Competitive

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  • 揮発性全身麻酔薬の副作用の分子機構:モーター蛋白1分子レベルでの解明機構解明

    2005 - 2007

    文部科学省  科学研究費補助金(基盤研究(C)) 

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    Grant type:Competitive

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  • ナノシステム内情報伝達の制御メカニズム

    2004 - 2006

    文部科学省  科学研究費補助金(特定領域研究) 

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    Grant type:Competitive

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  • アクトミオシン動作機構におけるアクチンのポテンシャル変化の役割

    2004 - 2005

    文部科学省  科学研究費補助金(基盤研究(C)) 

    岩根 敦子

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    Authorship:Principal investigator  Grant type:Competitive

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  • アクトミオシン動作機構におけるアクチンの重要な役割

    2002 - 2003

    文部科学省  科学研究費補助金(基盤研究(C)) 

    岩根 敦子

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    Authorship:Principal investigator  Grant type:Competitive

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  • 細胞内1分子計測法を用いた走化性情報処理システムの解析

    2001 - 2005

    文部科学省  科学研究費補助金(基盤研究(S)) 

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    Grant type:Competitive

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  • ミオシンの運動メカニズムにおけるアクチンの重要な役割

    2000 - 2001

    文部科学省  科学研究費補助金(基盤研究(C)) 

    岩根 敦子

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    Authorship:Principal investigator  Grant type:Competitive

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  • 低分子量G蛋白質の活性化状態の可視化と細胞形態変化

    1999

    文部科学省  科学研究費補助金(特定領域研究(C)) 

    岩根 敦子

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    Authorship:Principal investigator  Grant type:Competitive

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  • 活性化状態における蛋白質の有効温度

    1998 - 2000

    文部科学省  科学研究費補助金(基盤研究(A)) 

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    Grant type:Competitive

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  • 1分子機能イメージング顕微鏡の開発と細胞内情報伝達研究への応用

    1998 - 2000

    文部科学省  科学研究費補助金(基盤研究(A)) 

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    Grant type:Competitive

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  • GFP変異体を用いた蛋白分子2量体形成の可視化と細胞内情報伝達研究への応用

    1998 - 1999

    文部科学省  科学研究費補助金(基盤研究(C)) 

    岩根 敦子

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    Authorship:Principal investigator  Grant type:Competitive

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  • 低分子量G蛋白質の活性化状態の可視化と細胞形態変化への関与

    1998

    文部科学省  科学研究費補助金(特定領域研究(C)) 

    岩根 敦子

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    Authorship:Principal investigator  Grant type:Competitive

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  • ミオシンモータードメイン-GFPキメラ蛋白の1分子機能アッセイ

    1996 - 1997

    文部科学省  科学研究費補助金(基盤研究(C)) 

    岩根 敦子

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    Authorship:Principal investigator  Grant type:Competitive

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  • Study on Mechanism of Actomyosin interaction

    1993

    Grants and Funding 

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    Grant type:Competitive

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