Updated on 2022/03/17

写真a

 
KAMIOKA Hiroshi
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Professor
Position
Professor
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Degree

  • 歯学博士 ( 徳島大学 )

Research Interests

  • 歯の移動と骨改造

  • Tooth movement and bone remodeling

Research Areas

  • Life Science / Social dentistry

Education

  • The University of Tokushima    

    - 1993

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  • The University of Tokushima   歯学研究科   歯科矯正学

    - 1993

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    Country: Japan

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  • The University of Tokushima   歯学部  

    - 1989

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    Country: Japan

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  • The University of Tokushima    

    - 1989

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Research History

  • 広島大学歯学部 非常勤講師

    2021.4

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  • 岡山大学病院 教育・研究(歯科)担当副病院長

    2021.4

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  • 岡山大学 学術研究院 医歯薬学域 教授

    2021.4

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  • Dokkyo Medical University   口腔外科学講座

    2019.4

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  • Osaka University   School of Dentistry

    2017.4

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  • 岡山大学病院口唇裂・口蓋裂総合治療センター   センター長

    2015.5

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  • Tohoku University   Faculty of Dentistry

    2015.4

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  • Kyushu University   School of Dentistry

    2015.4

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  • 岡山大学病院小児頭蓋顔面形成センター   副センター長

    2014.2

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  • 岡山大学医歯薬学総合研究科 教授

    2014.2 - 2021.3

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  • 岡山県社会福祉審議会   岡山県社会福祉審議会臨時職員

    2013.4

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  • Kyoto University   Research Center for Nano Medical Engineering, Institute for Frontier Medical Sciences

    2011.4 - 2015.3

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  • Gifu University   Graduate School of Medicine

    2007.4 - 2008.3

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  • 岡山大学医歯薬学総合研究科   准教授

    2004.7 - 2014.1

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Professional Memberships

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Committee Memberships

  • NPO法人 科学映像館   アドバイザー  

    2022.1   

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  • NPO法人せとうち臨床遺伝研究会   理事  

    2021.3   

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    Committee type:Government

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  • 日本抗加齢医学会   評議委員  

    2020.6   

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  • 日本骨形態計測学会   第40回日本骨形態計測学会 大会長  

    2020.6   

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    Committee type:Academic society

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  • 日本口腔科学会   専門査読委員  

    2020.4   

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    Committee type:Academic society

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  • 中・四国矯正歯科学会   副会長  

    2020.4   

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    Committee type:Academic society

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  • 日本顕微鏡学会   奨励賞審査委員  

    2019.12 - 2020.8   

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  • 日本口蓋裂学会   評議委員  

    2019.6   

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    Committee type:Academic society

    日本口蓋裂学会

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  • 日本矯正歯科学会   卒後教育委員会委員  

    2018.4   

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    Committee type:Academic society

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  • 日本矯正歯科学会   編集委員会委員  

    2018.4 - 2022.3   

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    Committee type:Academic society

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  • 日本骨形態計測学会   理事  

    2017.6   

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    Committee type:Academic society

    日本骨形態計測学会

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  • 日本矯正歯科学会   学術委員会委員  

    2017.4   

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    Committee type:Academic society

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  • 日本顕微鏡学会   代議員  

    2017.4   

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    Committee type:Academic society

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  • 日本顕微鏡学会   論文賞選考委員会委員  

    2017.4 - 2018.3   

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    Committee type:Academic society

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  • 日本顕微鏡学会   和文誌「顕微鏡」編集委員  

    2016.6 - 2022.3   

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    Committee type:Academic society

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  • 日本骨代謝学会   広報委員会委員  

    2016.4 - 2019.6   

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    Committee type:Academic society

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  • 顎変形症学会   評議委員  

    2014.4   

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    Committee type:Academic society

    顎変形症学会

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  • 日本顕微鏡学会   関西支部 幹事  

    2013.4 - 2017.3   

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    Committee type:Academic society

    日本顕微鏡学会

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  • 日本骨形態計測学会   評議員  

    2009.6   

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    Committee type:Academic society

    日本骨形態計測学会

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  • 日本骨代謝学会   Editorial Board of Journal of Bone and Mineral Metabolism  

    2006.4   

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    Committee type:Academic society

    日本骨代謝学会

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  • 中・四国矯正歯科学会   編集委員長  

    2004.1 - 2005.12   

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    Committee type:Academic society

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Papers

  • Improvement of anterior disc displacement on the mandibular deviated side after intraoral vertical ramus osteotomy in a patient with facial asymmetry: a case report Reviewed

    Hirotaka Ueda, Naoki Oka, Tsuyoshi Shimo, Akira Sasaki, Takashi Yamashiro, Hiroshi Kamioka

    CLINICAL AND INVESTIGATIVE ORTHODONTICS   2022

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

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  • A morphometric analysis of the osteocyte canaliculus using applied automatic semantic segmentation by machine learning Reviewed

    Kaori Tabata, Mana Hashimoto, Haruka Takahashi, Ziyi Wang, Noriyuki Nagaoka, Toru Hara, Hiroshi Kamioka

    Journal of Bone and Mineral Metabolism   2022

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  • High-resolution image-based simulation reveals membrane strain concentration on osteocyte processes caused by tethering elements Reviewed

    Yuka Yokoyama, Yoshitaka Kameo, Hiroshi Kamioka, Taiji Adachi

    Biomechanics and Modeling in Mechanobiology   20 ( 6 )   2353 - 2360   2021.12

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>Osteocytes are vital for regulating bone remodeling by sensing the flow-induced mechanical stimuli applied to their cell processes. In this mechanosensing mechanism, tethering elements (TEs) connecting the osteocyte process with the canalicular wall potentially amplify the strain on the osteocyte processes. The ultrastructure of the osteocyte processes and canaliculi can be visualized at a nanometer scale using high-resolution imaging via ultra-high voltage electron microscopy (UHVEM). Moreover, the irregular shapes of the osteocyte processes and the canaliculi, including the TEs in the canalicular space, should considerably influence the mechanical stimuli applied to the osteocytes. This study aims to characterize the roles of the ultrastructure of osteocyte processes and canaliculi in the mechanism of osteocyte mechanosensing. Thus, we constructed a high-resolution image-based model of an osteocyte process and a canaliculus using UHVEM tomography and investigated the distribution and magnitude of flow-induced local strain on the osteocyte process by performing fluid–structure interaction simulation. The analysis results reveal that local strain concentration in the osteocyte process was induced by a small number of TEs with high tension, which were inclined depending on the irregular shapes of osteocyte processes and canaliculi. Therefore, this study could provide meaningful insights into the effect of ultrastructure of osteocyte processes and canaliculi on the osteocyte mechanosensing mechanism.

    DOI: 10.1007/s10237-021-01511-y

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    Other Link: https://link.springer.com/article/10.1007/s10237-021-01511-y/fulltext.html

  • Investigation of the molecular causes underlying physical abnormalities in Diamond‐Blackfan anemia patients with RPL5 haploinsufficiency Reviewed

    Yuko Fukui, Satoru Hayano, Noriaki Kawanabe, Ziyi Wang, Akira Shimada, Megumu K. Saito, Isao Asaka, Hiroshi Kamioka

    Pathology International   71 ( 12 )   803 - 813   2021.12

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    Authorship:Last author   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/pin.13168

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pin.13168

  • Roles for B[a]P and FICZ in subchondral bone metabolism and experimental temporomandibular joint osteoarthritis via the AhR/Cyp1a1 signaling axis Reviewed

    Yuri Yoshikawa, Takashi Izawa, Yusaku Hamada, Hiroko Takenaga, Ziyi Wang, Naozumi Ishimaru, Hiroshi Kamioka

    Scientific Reports   11 ( 1 )   2021.12

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    <title>Abstract</title>Bone loss due to smoking represents a major risk factor for fractures and bone osteoporosis. Signaling through the aryl hydrocarbon receptor (AhR) and its ligands contributes to both bone homeostasis and inflammatory diseases. It remains unclear whether the same AhR signaling axis affects the temporomandibular joint (TMJ). The aim of this study was to investigate possible mechanisms which mediate bone loss in the TMJ due to smoking. In particular, whether benzo[<italic>a</italic>]pyrene (B[<italic>a</italic>]P), a carcinogen of tobacco smoke, induces expression of the AhR target gene, Cyp1a1, in mandibular condyles. Possible functions of an endogenous ligand of FICZ, were also investigated in a TMJ-osteoarthritis (OA) mouse model. B[<italic>a</italic>]P was administered orally to wild-type and <italic>AhR</italic>−/− mice and bone metabolism was subsequently examined. TMJ-OA was induced in wild-type mice with forceful opening of the mouth. Therapeutic functions of FICZ were detected with μCT and histology. Exposure to B[<italic>a</italic>]P accelerated bone loss in the mandibular subchondral bone. This bone loss manifested with osteoclastic bone resorption and upregulated expression of Cyp1a1 in an <italic>AhR</italic>-dependent manner. In a mouse model of TMJ-OA, FICZ exhibited a dose-dependent rescue of mandibular subchondral bone loss by repressing osteoclast activity. Meanwhile, in vitro, pre-treatment with FICZ reduced RANKL-mediated osteoclastogenesis. B[<italic>a</italic>]P regulates mandibular subchondral bone metabolism via the Cyp1a1. The AhR ligand, FICZ, can prevent TMJ-OA by regulating osteoclast differentiation.

    DOI: 10.1038/s41598-021-94470-4

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    Other Link: http://www.nature.com/articles/s41598-021-94470-4

  • Extracellular vesicles of P. gingivalis-infected macrophages induce lung injury. International journal

    Kayo Yoshida, Kaya Yoshida, Natsumi Fujiwara, Mariko Seyama, Kisho Ono, Hotaka Kawai, Jiajie Guo, Ziyi Wang, Yao Weng, Yaqiong Yu, Yoko Uchida-Fukuhara, Mika Ikegame, Akira Sasaki, Hitoshi Nagatsuka, Hiroshi Kamioka, Hirohiko Okamura, Kazumi Ozaki

    Biochimica et biophysica acta. Molecular basis of disease   1867 ( 11 )   166236 - 166236   2021.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    Periodontal diseases are common inflammatory diseases that are induced by infection with periodontal bacteria such as Porphyromonas gingivalis (Pg). The association between periodontal diseases and many types of systemic diseases has been demonstrated; the term "periodontal medicine" is used to describe how periodontal infection/inflammation may impact extraoral health. However, the molecular mechanisms by which the factors produced in the oral cavity reach multiple distant organs and impact general health have not been elucidated. Extracellular vesicles (EVs) are nano-sized spherical structures secreted by various types of cells into the tissue microenvironment, and influence pathophysiological conditions by delivering their cargo. However, a detailed understanding of the effect of EVs on periodontal medicine is lacking. In this study, we investigated whether EVs derived from Pg-infected macrophages reach distant organs in mice and influence the pathophysiological status. EVs were isolated from human macrophages, THP-1 cells, infected with Pg. We observed that EVs from Pg-infected THP-1 cells (Pg-inf EVs) contained abundant core histone proteins such as histone H3 and translocated to the lungs, liver, and kidneys of mice. Pg-inf EVs also induced pulmonary injury, including edema, vascular congestion, inflammation, and collagen deposition causing alveoli destruction. The Pg-inf EVs or the recombinant histone H3 activated the NF-κB pathway, leading to increase in the levels of pro-inflammatory cytokines in human lung epithelial A549 cells. Our results suggest a possible mechanism by which EVs produced in periodontal diseases contribute to the progression of periodontal medicine.

    DOI: 10.1016/j.bbadis.2021.166236

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  • O‐GlcNAcylation drives calcium signaling toward osteoblast differentiation: A bioinformatics‐oriented study Reviewed

    Yao Weng, Ziyi Wang, Yoko Fukuhara, Airi Tanai, Mika Ikegame, Daisuke Yamada, Takeshi Takarada, Takashi Izawa, Satoru Hayano, Kaya Yoshida, Hiroshi Kamioka, Hirohiko Okamura

    BioFactors   47 ( 6 )   992 - 1015   2021.11

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    Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/biof.1774

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/biof.1774

  • RFX1‐mediated CCN3 induction that may support chondrocyte survival under starved conditions Reviewed

    Tomomi Mizukawa, Takashi Nishida, Sho Akashi, Kazumi Kawata, Sumire Kikuchi, Harumi Kawaki, Masaharu Takigawa, Hiroshi Kamioka, Satoshi Kubota

    Journal of Cellular Physiology   236 ( 10 )   6884 - 6896   2021.10

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    Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/jcp.30348

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jcp.30348

  • Quantitative Evaluation of Osteocyte Morphology and Bone Anisotropic Extracellular Matrix in Rat Femur Reviewed

    Takuya Ishimoto, Keita Kawahara, Aira Matsugaki, Hiroshi Kamioka, Takayoshi Nakano

    Calcified Tissue International   109 ( 4 )   434 - 444   2021.10

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>Osteocytes are believed to play a crucial role in mechanosensation and mechanotransduction which are important for maintenance of mechanical integrity of bone. Recent investigations have revealed that the preferential orientation of bone extracellular matrix (ECM) mainly composed of collagen fibers and apatite crystallites is one of the important determinants of bone mechanical integrity. However, the relationship between osteocytes and ECM orientation remains unclear. In this study, the association between ECM orientation and anisotropy in the osteocyte lacuno-canalicular system, which is thought to be optimized along with the mechanical stimuli, was investigated using male rat femur. The degree of ECM orientation along the femur longitudinal axis was significantly and positively correlated with the anisotropic features of the osteocyte lacunae and canaliculi. At the femur middiaphysis, there are the osteocytes with lacunae that highly aligned along the bone long axis (principal stress direction) and canaliculi that preferentially extended perpendicular to the bone long axis, and the highest degree of apatite <italic>c</italic>-axis orientation along the bone long axis was shown. Based on these data, we propose a model in which osteocytes can change their lacuno-canalicular architecture depending on the mechanical environment so that they can become more susceptible to mechanical stimuli via fluid flow in the canalicular channel.

    DOI: 10.1007/s00223-021-00852-1

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    Other Link: https://link.springer.com/article/10.1007/s00223-021-00852-1/fulltext.html

  • Authors’ Response to Letter from Professor Birkedal Reviewed

    Takuya Ishimoto, Hiroshi Kamioka, Takayoshi Nakano

    Calcified Tissue International   2021.8

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    DOI: 10.1007/s00223-021-00901-9

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    Other Link: https://link.springer.com/article/10.1007/s00223-021-00901-9/fulltext.html

  • 歯科矯正用アンカースクリューを用いて上下顎前歯の圧下により治療したガミースマイルを示す過蓋咬合症例 Reviewed

    片岡伴記, 星島光博, 上岡寛

    中・四国矯正歯科学会雑誌   33 ( 1 )   29 - 38   2021.8

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  • Index of Orthognathic Functional Treatment Need (IOFTN)とSeverity and Outcome Assessment (SOA)を用いた外科的矯正治療の適応基準の検討:ケースコントロール研究 Reviewed

    中村政裕, 金光恵, 石田朋子, 中西泰之, 岡直毅, 藤澤厚郎, 川邉紀章, 上岡寛

    中・四国矯正歯科学会雑誌   33 ( 1 )   1 - 8   2021.8

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  • A skeletal Class III facial asymmetry case with a canted occlusal plane treated by LeFort I with unilateral horseshoe osteotomy Reviewed

    Tomoyo Tanaka, Mitsuhiro Hoshijima, Norie Yoshioka, Hiroshi Kamioka

    Orthodontic Waves   80 ( 3 )   171 - 183   2021.7

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Informa UK Limited  

    DOI: 10.1080/13440241.2021.1952392

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  • 下垂体機能低下を伴う中隔視神経異形成症患者の矯正歯科治療症例 Reviewed

    河野加奈, 柳田剛志, 井澤俊, 上岡寛

    岡山歯学会雑誌   40 ( 1 )   1 - 10   2021.6

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  • Endonuclease increases efficiency of osteoblast isolation from murine calvariae Reviewed

    Yosuke Asano, Yoshinori Matsumoto, Jose La Rose, Fang He, Takayuki Katsuyama, Wang Ziyi, Shigetomo Tsuji, Hiroshi Kamioka, Robert Rottapel, Jun Wada

    Scientific Reports   11 ( 1 )   8502   2021.4

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>Bone is a highly dynamic organ that undergoes remodeling equally regulated by osteoblast-mediated bone formation and osteoclast-mediated bone resorption. To clarify the regulation of osteoblastogenesis, primary murine osteoblasts are required for an in vitro study. Primary osteoblasts are isolated from neonatal calvariae through digestion with collagenase. However, the number of cells collected from one pup is not sufficient for further in vitro experiments, leading to an increase in the use of euthanized pups. We hypothesized that the viscosity of digested calvariae and digestion solution supplemented with collagenase results in cell clumping and reduction of isolated cells from bones. We simply added Benzonase, a genetically engineered endonuclease that shears all forms of DNAs/RNAs, in order to reduce nucleic acid-mediated viscosity. We found that addition of Benzonase increased the number of collected osteoblasts by three fold compared to that without Benzonase through reduction of viscosity. Additionally, Benzonase has no effect on cellular identity and function. The new osteoblast isolation protocol with Benzonase minimizes the number of neonatal pups required for an in vitro study and expands the concept that isolation of other populations of cells including osteocytes that are difficult to be purified could be modified by Benzonase.

    DOI: 10.1038/s41598-021-87716-8

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    Other Link: http://www.nature.com/articles/s41598-021-87716-8

  • Camouflage treatment for skeletal maxillary protrusion and lateral deviation with classic-type Ehlers-Danlos syndrome. Reviewed

    Hoshijima M, Kawanabe N, Iida S, Yamashiro T, Kamioka H

    Acta Medica Okayama   75 ( 2 )   205 - 212   2021.4

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  • Changes in the intra- and peri-cellular sclerostin distribution in lacuno-canalicular system induced by mechanical unloading Reviewed

    Ryuta Osumi, Ziyi Wang, Yoshihito Ishihara, Naoya Odagaki, Tadahiro Iimura, Hiroshi Kamioka

    Journal of Bone and Mineral Metabolism   39 ( 2 )   148 - 159   2021.3

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00774-020-01135-9

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    Other Link: https://link.springer.com/article/10.1007/s00774-020-01135-9/fulltext.html

  • Age-related changes in the effect of rapid maxillary expansion on the position of labially impacted maxillary canines: A case-control study Reviewed

    Momoko Harada-Karashima, Yoshihito Ishihara, Hiroshi Kamioka, Ryuzo Kanomi

    American Journal of Orthodontics and Dentofacial Orthopedics   159 ( 3 )   305 - 311   2021.3

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ajodo.2019.10.026

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  • RFX1‐mediated CCN3 induction that may support chondrocyte survival under starved conditions Reviewed

    Tomomi Mizukawa, Takashi Nishida, Sho Akashi, Kazumi Kawata, Sumire Kikuchi, Harumi Kawaki, Masaharu Takigawa, Hiroshi Kamioka, Satoshi Kubota

    Journal of Cellular Physiology   2021.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/jcp.30348

  • Simultaneous distal movement of the maxillary first and second premolars can be achieved by distal movement of the maxillary first molar using a headgear Reviewed

    Junichi Miyano, Noriaki Kawanabe, Atsushi Fujishiro, Ryuzo Kanomi, Hiroshi Kamioka

    Orthodontic Waves   79 ( 4 )   171 - 178   2020.11

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    Authorship:Last author   Publishing type:Research paper (scientific journal)   Publisher:Informa UK Limited  

    DOI: 10.1080/13440241.2020.1845587

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  • Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes Reviewed

    Ziyi Wang, Yao Weng, Yoshihito Ishihara, Naoya Odagaki, Ei Ei Hsu Hlaing, Takashi Izawa, Hirohiko Okamura, Hiroshi Kamioka

    PeerJ   8   e10244 - e10244   2020.11

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    <sec>
    <title>Background</title>
    In this study, we investigated the effect of the mechanical loading history on the expression of receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) in MLO-Y4 osteocyte-like cells.


    </sec>
    <sec>
    <title>Methods</title>
    Three hours after MLO-Y4 osteocytes were seeded, a continuous compressive force (CCF) of 31 dynes/cm2 with or without additional CCF (32 dynes/cm2) was loaded onto the osteocytes. After 36 h, the additional CCF (loading history) was removed for a recovery period of 10 h. The expression of RANKL, OPG, RANKL/OPG ratio, cell numbers, viability and morphology were time-dependently examined at 0, 3, 6 and 10 h. Then, the same additional CCF was applied again for 1 h to all osteocytes with or without the gap junction inhibitor to examine the expression of RANKL, OPG, the RANKL/OPG ratio and other genes that essential to characterize the phenotype of MLO-Y4 cells. Fluorescence recovery after photobleaching technique was also applied to test the differences of gap-junctional intercellular communications (GJIC) among MLO-Y4 cells.


    </sec>
    <sec>
    <title>Results</title>
    The expression of RANKL and OPG by MLO-Y4 osteocytes without a loading history was dramatically decreased and increased, respectively, in response to the 1-h loading of additional weight. However, the expression of RANKL, OPG and the RANKL/OPG ratio were maintained at the same level as in the control group in the MLO-Y4 osteocytes with a loading history but without gap junction inhibitor treatment. Treatment of loading history significantly changed the capacity of GJIC and protein expression of connexin 43 (Cx43) but not the mRNA expression of Cx43. No significant difference was observed in the cell number or viability between the MLO-Y4 osteocyte-like cells with and without a loading history or among different time checkpoints during the recovery period. The cell morphology showed significant changes and was correlated with the expression of OPG, Gja1 and Dmp1 during the recovery period.


    </sec>
    <sec>
    <title>Conclusion</title>
    Our findings indicated that the compressive force-induced changes in the RANKL/OPG expression could be habituated within at least 11 h by 36-h CCF exposure. GJIC and cell morphology may play roles in response to loading history in MLO-Y4 osteocyte-like cells.


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    DOI: 10.7717/peerj.10244

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    Other Link: https://peerj.com/articles/10244.xml

  • Three-dimensional changes in the craniofacial complex associated with soft-diet feeding Reviewed

    Kana Kono, Chihiro Tanikawa, Yuka Murata, Takeshi Yanagita, Hiroshi Kamioka, Takashi Yamashiro

    European Journal of Orthodontics   42 ( 5 )   509 - 516   2020.11

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    Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    <title>Summary</title>
    <sec>
    <title>Background and objectives</title>
    The masticatory force affects craniofacial development. We aimed to quantify the topological deviation of the growing craniofacial structure due to soft-food diet feeding and to map the region where the phenotypes appeared on three-dimensional (3D) images.


    </sec>
    <sec>
    <title>Material and methods</title>
    Mice were fed a powdered soft diet (SD) or conventional hard diet (HD) of regular rodent pellets at 3 weeks of age until 9 weeks of age. The heads, excluding the mandibles, were scanned by micro-computed tomography. The topographic deviation of the bony surface was quantitatively assessed by a wire mesh fitting analysis. The actual displacement and significant differences were mapped and visualized in each x-, y-, and z-axis on the 3D craniofacial image. On these reconstructed images, two-dimensional linear measurements between the landmark points confirmed the 3D skeletal displacement.


    </sec>
    <sec>
    <title>Results</title>
    In the transverse direction, the zygomatic arches and the region in which the temporal muscle attaches to the parietal and temporal bones were narrow in the SD group. The temporal muscle attachment regions significantly shifted anteriorly, and consequently, the sagittal zygomatic arch shortened. Although the cranial sagittal length was not affected, the vertical height was also reduced in the SD group compared to the HD group.


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    <title>Conclusions</title>
    Our 3D surface-based analysis demonstrated that SD feeding resulted in reduced 3D bony development at the region where the chewing muscles attach to the zygomatic arches and the temporal and parietal bones. Interestingly, SD feeding induced an anterior shift in the temporal and parietal bone regions, which can affect the skeletal inter-jaw relationship.


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  • The expression and regulation of Wnt1 in tooth movement-initiated mechanotransduction. International journal

    Ei Ei Hsu Hlaing, Yoshihito Ishihara, Naoya Odagaki, Ziyi Wang, Mika Ikegame, Hiroshi Kamioka

    American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics   158 ( 6 )   e151 - e159   2020.10

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    INTRODUCTION: The Wnt signaling pathway acts as a key regulator of skeletal development and its homeostasis. However, the potential role of Wnt1 in the mechanotransduction machinery of orthodontic tooth movement-initiated bone remodeling is still unclear. Hence, this study focused on the regulatory dynamics of the Wnt1 expression in both the periodontal ligament (PDL) and osteocytes in vivo and in vitro. METHODS: The Wnt1 expression in the orthodontically moved maxillary first molar in mice was assessed at 0, 1, and 5 days, on both the compression and tension sides. Primary isolated human PDL (hPDL) fibroblasts, as well as murine long-bone osteocyte-Y4 (MLO-Y4) cells, were exposed to continuous compressive force and static tensile force. RESULTS: The relative quantification of immunodetection showed that orthodontic tooth movement significantly stimulated the Wnt1 expression in both the PDL and alveolar osteocytes on the tension side on day 5, whereas the expression on the compression side did not change. This increase in the Wnt1 expression, shown in vivo, was also noted after the application of 12% static tensile force in isolated hPDL fibroblasts and 20% in MLO-Y4 cells. In contrast, a compressive force led to the attenuation of the Wnt1 gene expression in both hPDL fibroblasts and MLO-Y4 cells in a force-dependent manner. In the osteocyte-PDL coculture system, recombinant sclerostin attenuated Wnt1 in PDL, whereas the antisclerostin antibody upregulated its gene expression, indicating that mechanically-driven Wnt1 signaling in PDL might be regulated by osteocytic sclerostin. CONCLUSIONS: Our findings provide that Wnt1 signaling plays a vital role in tooth movement-initiated bone remodeling via innovative mechanotransduction approaches.

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  • Outer membrane vesicles derived from Porphyromonas gingivalis induced cell death with disruption of tight junctions in human lung epithelial cells

    Yuhan He, Noriko Shiotsu, Yoko Uchida-Fukuhara, Jiajie Guo, Yao Weng, Mika Ikegame, Ziyi Wang, Kisho Ono, Hiroshi Kamioka, Yasuhiro Torii, Akira Sasaki, Kaya Yoshida, Hirohiko Okamura

    Archives of Oral Biology   118   104841 - 104841   2020.10

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  • N-(3-oxododecanoyl)-homoserine lactone regulates osteoblast apoptosis and differentiation by mediating intracellular calcium. International journal

    Jiajie Guo, Ziyi Wang, Yao Weng, Haoze Yuan, Kaya Yoshida, Mika Ikegame, Kenta Uchibe, Hiroshi Kamioka, Kazuhiko Ochiai, Hirohiko Okamura, Lihong Qiu

    Cellular signalling   75   109740 - 109740   2020.8

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    Pseudomonas aeruginosa (P. aeruginosa) is associated with periapical periodontitis. The lesions are characterized by a disorder in osteoblast metabolism. Quorum sensing molecular N-(3-oxododecanoyl)-homoserine lactone (AHL) is secreted by P. aeruginosa and governs the expression of numerous virulence factors. AHL can trigger intracellular calcium ([Ca2+]i) fluctuations in many host cells. However, it is unclear whether AHL can regulate osteoblast metabolism by affecting [Ca2+]i changes or its spatial correlation. We explored AHL-induced apoptosis and differentiation in pre-osteoblastic MC3T3-E1 cells and evaluated [Ca2+]i mobilization using several extraction methods. The spatial distribution pattern of [Ca2+]i among cells was investigated by Moran's I, an index of spatial autocorrelation. We found that 30 μM and 50 μM AHL triggered opposing osteoblast fates. At 50 μM, AHL inhibited osteoblast differentiation by promoting mitochondrial-dependent apoptosis and negatively regulating osteogenic marker genes, including Runx2, Osterix, bone sialoprotein (Bsp), and osteocalcin (OCN). In contrast, prolonged treatment with 30 μM AHL promoted osteoblast differentiation concomitantly with cell apoptosis. The elevation of [Ca2+]i levels in osteoblasts treated with 50 μM AHL was spatially autocorrelated, while no such phenomenon was observed in 30 μM AHL-treated osteoblasts. The blocking of cell-to-cell spatial autocorrelation in the osteoblasts provoked by 50 μM AHL significantly inhibited apoptosis and partially restored differentiation. Our observations suggest that AHL affects the fate of osteoblasts (apoptosis and differentiation) by affecting the spatial correlation of [Ca2+]i changes. Thus, AHL acts as a double-edged sword for osteoblast function.

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  • 上下顎骨骨切り術により顎運動の改善が見られた顎関節症を併発する骨格性下顎前突症例 Reviewed

    菅原康代, 石原嘉人, 上岡 寛

    中・四国矯正歯科学会雑誌   32 ( 1 )   39 - 50   2020.8

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  • 歯科矯正用アンカースクリューを用いて上顎前歯および臼歯の圧下を行ったガミースマイルを伴う過蓋咬合症例 Reviewed

    早野 暁, 宮本久美, 上岡 寛

    中・四国矯正歯科学会雑誌   32 ( 1 )   17 - 24   2020.8

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  • 下顎枝垂直骨切り術後に顎関節症状の改善と偏位側への側方滑走運動の改善を認めた顔面非対称症例 Reviewed

    植田紘貴, 岡直毅, 上岡 寛

    中・四国矯正歯科学会雑誌   32 ( 1 )   51 - 63   2020.8

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  • 岡山大学病院矯正歯科における永久歯先天欠如に関する調査および矯正歯科治療症例 Reviewed

    星島光博, 出射明美, 福井裕子, 鳥原秀美, 松田祐典, 上岡寛

    岡山歯学会雑誌   39 ( 2 )   19 - 25   2020.8

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  • Occlusal reconstruction of a patient with ameloblastoma ablation using alveolar distraction osteogenesis: a case report. Reviewed International journal

    Yoshihito Ishihara, Hikaru Arakawa, Akiyoshi Nishiyama, Hiroshi Kamioka

    Head & face medicine   16 ( 1 )   12 - 12   2020.6

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    BACKGROUND: Ameloblastoma is one of the most common benign odontogenic neoplasms. Its surgical excision has the potential to lead to postoperative malocclusion. In this case report, we describe the successful interdisciplinary orthodontic treatment of a patient with ameloblastoma who underwent marginal mandibulectomy. CASE PRESENTATION: A woman of 20-year-old was diagnosed with ameloblastoma, and underwent marginal mandibulectomy when she was 8 years of age. She had an excessive overjet (11.5 mm) and a mild open bite (- 1.5 mm) with a severely resorbed atrophic edentulous ridge in the area around the mandibular left lateral incisor, canine and first premolar. An alveolar bone defect associated with tumor resection was regenerated by vertical distraction osteogenesis (DO). Subsequently, 3 dental implants were placed into the reconstructed mandible. Orthodontic treatment using implant-anchored mechanics provided a proper facial profile with significantly improved occlusal function. The occlusion appeared stable for a 7-year retention period. CONCLUSIONS: These results suggest that surgically assisted and implant anchored-orthodontic approaches might be effective for the correction of such malocclusions.

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  • Comprehensive approach to simultaneous molar intrusion and canine retraction in the treatment of Class II anterior open bite using miniscrew anchorage Reviewed

    Kaori Shirasaki, Yoshihito Ishihara, Hiroki Komori, Takashi Yamashiro, Hiroshi Kamioka

    Dental Press Journal of Orthodontics   25 ( 3 )   2020.5

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    ABSTRACT Introduction: Anterior open bite is one of the most difficult malocclusions to correct in orthodontic treatment. Molar intrusion using miniscrew anchorage has been developed as a new strategy for open bite correction; however, this procedure still has an important concern about prolonged treatment duration in the patient with anteroposterior discrepancy due to the separate step-by-step movement of anterior and posterior teeth. Objective: This article illustrates a comprehensive orthodontic approach for dentoalveolar open bite correction of an adult patient, by using miniscrew. Case report: A woman 19 years and 5 months of age had chief complaints of difficulty chewing with the anterior teeth and maxillary incisor protrusion. An open bite of -2.0 mm caused by slight elongation of the maxillary molars was found. The patient was diagnosed with Angle Class II malocclusion with anterior open bite due to the vertical elongation of maxillary molars. After extraction of the maxillary first premolars, concurrent movements of molar intrusion and canine retraction were initiated with the combined use of sectional archwires, elastic chains and miniscrews. Results: At 4 months after the procedure, positive overbite was achieved subsequent to the intrusion of maxillary molars by 1.5 mm and without undesirable side effects. Class I canine relation was also achieved at the same time. The total active treatment period was 21 months. The resultant occlusion and satisfactory facial profile were maintained after 54 months of retention. Conclusion: The presented treatment shows the potential to shorten the treatment duration and to contribute to the long-term stability for open bite correction.

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  • Roles of Interaction between CCN2 and Rab14 in Aggrecan Production by Chondrocytes. Reviewed International journal

    Mitsuhiro Hoshijima, Takako Hattori, Eriko Aoyama, Takashi Nishida, Satoshi Kubota, Hiroshi Kamioka, Masaharu Takigawa

    International journal of molecular sciences   21 ( 8 )   2020.4

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    To identify proteins that cooperate with cellular communication network factor 2 (CCN2), we carried out GAL4-based yeast two-hybrid screening using a cDNA library derived from the chondrocytic cell line HCS-2/8. Rab14 GTPase (Rab14) polypeptide was selected as a CCN2-interactive protein. The interaction between CCN2 and Rab14 in HCS-2/8 cells was confirmed using the in situ proximity ligation assay. We also found that CCN2 interacted with Rab14 through its IGFBP-like domain among the four domains in CCN2 protein. To detect the colocalization between CCN2 and Rab14 in the cells in detail, CCN2, wild-type Rab14 (Rab14WT), a constitutive active form (Rab14CA), and a dominant negative form (Rab14DN) of Rab14 were overexpressed in monkey kidney-tissue derived COS7 cells. Ectopically overexpressed Rab14 showed a diffuse cytosolic distribution in COS7 cells; however, when Rab14WT was overexpressed with CCN2, the Rab14WT distribution changed to dots that were evenly distributed within the cytosol, and both Rab14 and CCN2 showed clear colocalization. When Rab14CA was overexpressed with CCN2, Rab14CA and CCN2 also showed good localization as dots, but their distribution was more widespread within cytosol. The coexpression of Rab14DN and CCN2 also showed a dotted codistribution but was more concentrated in the perinuclear area. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed that the reduction in RAB14 or CCN2 mRNA by their respective siRNA significantly enhanced the expression of ER stress markers, BIP and CHOP mRNA in HCS-2/8 chondrocytic cells, suggesting that ER and Golgi stress were induced by the inhibition of membrane vesicle transfer via the suppression of CCN2 or Rab14. Moreover, to study the effect of the interaction between CCN2 and its interactive protein Rab14 on proteoglycan synthesis, we overexpressed Rab14WT or Rab14CA or Rab14DN in HCS-2/8 cells and found that the overexpression of Rab14DN decreased the extracellular proteoglycan accumulation more than the overexpression of Rab14WT/CA did in the chondrocytic cells. These results suggest that intracellular CCN2 is associated with Rab14 on proteoglycan-containing vesicles during their transport from the Golgi apparatus to endosomes in chondrocytes and that this association may play a role in proteoglycan secretion by chondrocytes.

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  • Inhibitory effect of retinoic acid receptor agonists on in vitro chondrogenic differentiation. Reviewed

    Yusuke Sumitani, Kenta Uchibe, Kaya Yoshida, Yao Weng, Jiajie Guo, Haoze Yuan, Mika Ikegame, Hiroshi Kamioka, Hirohiko Okamura

    Anatomical science international   95 ( 2 )   202 - 208   2020.3

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    Retinoic acid (RA), an active metabolite of vitamin A, plays pivotal roles in a wide variety of biological processes, such as body patterning, organ development, and cell differentiation and proliferation. RA signaling is mediated by nuclear retinoic acid receptors, α, β, and γ (RARα, RARβ, and RARγ). RA is a well-known regulator of cartilage and skeleton formation and RARs are also essential for skeletal growth and hypertrophic chondrocyte-specific gene expression. These important roles of RA and RARs in chondrogenesis have been widely investigated using in vivo mouse models. However, few reports are available on the function of each subtype of RARs on in vitro chondrocyte differentiation. Here, we examined the effect of specific agonists of RARs on chondrogenic differentiation of ATDC5 and C3H10T1/2 cells. Subtype-specific RAR agonists as well as RA decreased the expressions of chondrogenic differentiation marker genes and inhibited chondrogenic differentiation, which was accompanied with morphological change to spindle-shaped cells. Among RAR agonists, RARα and RARγ agonists revealed a strong inhibitory effect on chondrogenic differentiation. RARα and RARγ agonists also hampered viability of ATDC5 cells. These observations suggested that RARα and RARγ are dominant receptors of RA signaling that negatively regulate chondrogenic differentiation.

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  • Orthodontic management of a non-syndromic patient with concomitant bimaxillary hypohyperdontia: a case report Reviewed

    Ei Ei Hsu Hlaing, Yoshihito Ishihara, Atsuro Fujisawa, Takashi Yamashiro, Hiroshi Kamioka

    Dental Press Journal of Orthodontics   25 ( 1 )   36 - 46   2020.1

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    ABSTRACT Introduction: Tooth agenesis is one of the most common dental anomalies; however, the concomitant occurrence of opposite dental numerical variation of hypohyperdontia is extremely rare. Objective: To report the successful orthodontic management of a patient with non-syndromic concomitant bilateral agenesis of mandibular canines and two midline inverted supernumerary maxillary teeth. Case report: 21-year-old female patient with a chief complaint of protrusive right maxillary central incisor. The patient was diagnosed with a mild Class II skeletal base, Angle Class III molar relationship and increased overjet associated with hypohyperdontia. Anterior open bite accompanied with tongue-thrusting habit were also observed. Two temporary anchorage devices (TADs) were implanted at the buccal side of the maxillary molar region to control vertical height. Anterior teeth retraction was done after extraction of the maxillary first premolars, to improve the excessive overjet. The treatment mechanics involved lingual brackets system for the maxillary arch and transpalatal arch for anchorage control. Results: The total active treatment period was 35 months. Acceptable occlusion with increased bite force and contact area as well as functional excursion were established without interference, following complex orthodontic treatment with premolar substitution. The resultant occlusion and a satisfactory facial profile were maintained after 29 months of retention. Conclusion: The present case report provides implications regarding the orthodontic treatment of hypohyperdontia-associated substitution for missing teeth as an effective option for improving aesthetic and functional aspects.

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  • The Analysis of Gap Junctional Intercellular Communication Among Osteocytes in Chick Calvariae by Fluorescence Recovery After Photobleaching Invited Reviewed

    Ziyi Wang, Yoshihito Ishihara, Hiroshi Kamioka

    Methods in Molecular Biology   215 - 223   2020

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  • 歯科矯正用アンカースクリューによりデンタルディコンペンセーションを図った骨格性III級症例に対する外科的矯正治療 Invited Reviewed

    田中智代, 村上隆, 森谷徳文, 松村達志, 飯田征二, 上岡 寛

    日本顎変形症学会雑誌   29 ( 4 )   295 - 303   2020

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  • Mechanical regulation of bone homeostasis through p130Cas-mediated alleviation of NF-κB activity Reviewed

    T. Miyazaki, Z. Zhao, Y. Ichihara, D. Yoshino, T. Imamura, K. Sawada, S. Hayano, H. Kamioka, S. Mori, H. Hirata, K. Araki, K. Kawauchi, K. Shigemoto, S. Tanaka, L. F. Bonewald, H. Honda, M. Shinohara, M. Nagao, T. Ogata, I. Harada, Y. Sawada

    Science Advances   5 ( 9 )   eaau780   2020

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  • フッ素イオンによるCCNファミリー遺伝子の制御

    水川 朋美, 西田 崇, 明石 翔, 堀 彩花, 高柴 正悟, 上岡 寛, 滝川 正春, 久保田 聡

    岡山歯学会雑誌   38 ( 2 )   85 - 85   2019.12

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  • Role of intracellular Ca2+-based mechanotransduction of human periodontal ligament fibroblasts Reviewed

    Ei Hsu, Hlaing E, Ishihara Y, Wang Z, Odagaki N, Kamioka H

    FASEB J   33 ( 9 )   10409 - 10424   2019.9

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  • Screening of key candidate genes and pathways for osteocytes involved in the differential response to different types of mechanical stimulation using a bioinformatics analysis. Reviewed

    Ziyi Wang, Yoshihito Ishihara, Takanori Ishikawa, Mitsuhiro Hoshijima, Naoya Odagaki, Ei Ei Hsu Hlaing, Hiroshi Kamioka

    Journal of bone and mineral metabolism   37 ( 4 )   614 - 626   2019.7

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    This study aimed to predict the key genes and pathways that are activated when different types of mechanical loading are applied to osteocytes. mRNA expression datasets (series number of GSE62128 and GSE42874) were obtained from Gene Expression Omnibus database (GEO). High gravity-treated osteocytic MLO-Y4 cell-line samples from GSE62128 (Set1), and fluid flow-treated MLO-Y4 samples from GSE42874 (Set2) were employed. After identifying the differentially expressed genes (DEGs), functional enrichment was performed. The common DEGs between Set1 and Set2 were considered as key DEGs, then a protein-protein interaction (PPI) network was constructed using the minimal nodes from all of the DEGs in Set1 and Set2, which linked most of the key DEGs. Several open source software programs were employed to process and analyze the original data. The bioinformatic results and the biological meaning were validated by in vitro experiments. High gravity and fluid flow induced opposite expression trends in the key DEGs. The hypoxia-related biological process and signaling pathway were the common functional enrichment terms among the DEGs from Set1, Set2 and the PPI network. The expression of almost all the key DEGs (Pdk1, Ccng2, Eno2, Egln1, Higd1a, Slc5a3 and Mxi1) were mechano-sensitive. Eno2 was identified as the hub gene in the PPI network. Eno2 knockdown results in expression changes of some other key DEGs (Pdk1, Mxi1 and Higd1a). Our findings indicated that the hypoxia response might have an important role in the differential responses of osteocytes to the different types of mechanical force.

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  • Biomimetic mineralization using matrix vesicle nanofragments. Reviewed International journal

    Yosuke Kunitomi, Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takuo Kuboki, Takayoshi Nakano, Hiroshi Kamioka, Takuya Matsumoto

    Journal of biomedical materials research. Part A   107 ( 5 )   1021 - 1030   2019.5

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    In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate bone tissue are still ineffective due to some remaining gaps in the understanding of real in vivo bone formation process, and application of the knowledge in bone synthesis. Therefore, the objectives of this study were first, to perform a systematic and ultrastructural investigation of the initial mineral formation during intramembranous ossification of mouse calvaria from a material scientists' viewpoint, and to develop novel mineralization methods based on the in vivo findings. First, the very initial mineral deposition was found to occur at embryonic day E14.0 in mouse calvaria. Analysis of the initial bone formation process showed that it involved the following distinct steps: collagen secretion, matrix vesicle (MV) release, MV mineralization, MV rupture, and collagen fiber mineralization. Next, we performed in vitro mineralization experiments using MVs and hydrogel scaffolds. Intact MVs embedded in collagen gel did not mineralize, whereas, interestingly, MV nanofragments obtained by ultrasonication could promote rapid mineralization. These results indicate that mechanically ruptured MV membrane can be a promising material for in vitro bone tissue synthesis. © 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1021-1030, 2019.

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  • Nonsurgical orthodontic treatment of a hypodivergent adult patient with bilateral posterior scissors bite and excessive overjet. Reviewed International journal

    Nakamura M, Kawanabe N, Adachi R, Yamashiro T, Kamioka H

    Angle Orthodontist   89 ( 2 )   333 - 349   2019.3

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    This report illustrates successful nonsurgical orthodontic treatment of a hypodivergent adult patient with bilateral posterior scissors bite (Brodie bite) and excessive overjet. A 26-year-old woman primarily reported maxillary incisor protrusion. She was diagnosed with Class ll division 1 malocclusion with skeletal Class I, short face, low mandibular plane angle and bilateral posterior scissors bite. A lingual arch with anterior bite block and posterior miniscrews with preadjusted edgewise appliances were used to improve the bilateral scissors bite. After achieving molar occlusion, the maxillary first premolars were extracted, and six miniscrews were used to improve the anterior-posterior and vertical discrepancies. After active treatment for 56 months, the convex facial profile with excessively protruded lips was improved and good interdigitation with ideal incisor relationship was achieved. Additionally, the irregular movements of the incisal path and the bilateral condyles during lateral excursion were improved. At 13 months of retention, a satisfactory facial profile, occlusion, and jaw movements were maintained. The treatment results suggest that miniscrews and fixed bite blocks were effective and efficient to facilitate correction of the bilateral scissors bite, excessive overjet, and vertical relationship correction in this nonsurgical orthodontic treatment.

    File: Nakamura(AngleOrthod)2019.pdf

    DOI: 10.2319/111617-791.1

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  • 咬合平面傾斜と下顎両側側切歯先天欠如を伴う叢生症例に対し歯科矯正用アンカースクリューを併用し治療を行った1症例 Reviewed

    河野加奈, 柳田剛志, 上岡 寛

    中・四国矯正歯科学会雑誌   1 ( 1 )   39 - 48   2019

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  • Differential distribution of microtubules in immature osteocytes in vitro. Reviewed

    Murshid S, Takano-Yamamoto T, Kamioka H

    Journal of Oral Biosciences   60 ( 4 )   98 - 101   2018.12

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  • Orthodontic correction of severe Class II malocclusion in a patient with Prader-Willi syndrome. Reviewed International journal

    Ishihara Y, Sugawara Y, Ei Hsu, Hlaing E, Nasu M, Kataoka T, Odagaki N, Takano-Yamamoto T, Yamashiro T, Kamioka H

    Am J Orthod Dentofacial Orthop.   154 ( 5 )   718 - 732   2018.11

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    Prader-Willi syndrome (PWS) is a complex disorder that affects multiple systems and may cause craniofacial and dentofacial abnormalities. However, there is still a lack of evidence in the literature regarding the progress of orthodontic treatment in patients with PWS. This case report describes the successful orthodontic treatment of a patient with PWS. A girl, 9 years 0 months of age, who had been diagnosed with PWS had protruding maxillary incisors and a convex profile. Her malocclusion was due to the posteriorly positioned mandible. Screening tests for sleep apnea syndrome showed that she had sleep-disordered breathing, including obstructive sleep apnea and bruxism. We also observed an excessive overjet of 10.0 mm, a deep overbite of 6.8 mm, and the congenital absence of the mandibular second premolars. The patient was diagnosed with an Angle Class II malocclusion and a skeletal Class II jaw-base relationship with a deep overbite. Functional appliance therapy with mandibular advancement, which can enlarge the upper airway and increase the upper airspace, was performed to prevent further deterioration of the patient's obstructive sleep apnea. An acceptable occlusion with a proper facial profile and functional excursion were achieved without interference after comprehensive 2-stage treatment that incorporated orthodontic therapy for the patient's excessive overjet and deep overbite. The resulting occlusion was stable, and the occlusal force and the contact area gradually increased over a 2-year retention period. These results suggest that orthodontic treatment offers the opportunity to greatly improve the health and quality of life of people with PWS.

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  • Role of osteocyte-PDL crosstalk in tooth movement via SOST/Sclerostin Reviewed

    Odagaki N, Ishihara Y, Wang Z, Ei Hsu, Hlaing Ei, Nakamura M, Hoshimima M, Hayano S, Kawanabe N, Kamioka H

    Journal of Dental Research   97 ( 12 )   1374 - 1382   2018.11

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  • Analysis of Ca2+ response of osteocyte network by three-dimensional time-lapse imaging in living bone. Reviewed

    Tomoyo Tanaka, Mitsuhiro Hoshijima, Junko Sunaga, Takashi Nishida, Mana Hashimoto, Naoya Odagaki, Ryuta Osumi, Taiji Aadachi, Hiroshi Kamioka

    Journal of bone and mineral metabolism   36 ( 5 )   519 - 528   2018.9

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    Osteocytes form a three-dimensional (3D) cellular network within the mineralized bone matrix. The cellular network has important roles in mechanosensation and mechanotransduction related to bone homeostasis. We visualized the embedded osteocyte network in chick calvariae and observed the flow-induced Ca2+ signaling in osteocytes using 3D time-lapse imaging. In response to the flow, intracellular Ca2+ ([Ca2+]i) significantly increased in developmentally mature osteocytes in comparison with young osteocytes in the bone matrix. To investigate the differences in response between young and developmentally mature osteocytes in detail, we evaluated the expression of osteocyte-related genes using the osteocyte-like cell line MLO-Y4, which was 3D-cultured within type I collagen gels. We found that the c-Fos, Cx43, Panx3, Col1a1, and OCN mRNA levels significantly increased on day 15 in comparison with day 7. These findings indicate that developmentally mature osteocytes are more responsive to mechanical stress than young osteocytes and have important functions in bone formation and remodeling.

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  • Human gingival fibroblast feeder cells promote maturation of induced pluripotent stem cells into cardiomyocytes. Reviewed

    Matsuda Y, Takahashi K, Kamioka H, Naruse K

    Biochem Biophys Res Commun.   503 ( 3 )   1798 - 1804   2018.9

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  • 著しい骨格性下顎前突および多数歯欠如を伴う色素失調症患者に対して外科的矯正治療および補綴処置を行った一例 Reviewed

    石本和也, 柳田剛志, 川邉紀章, 上岡寛

    中・四国矯正歯科学会雑誌   30 ( 1 )   23 - 32   2018.8

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  • Role of the inferior alveolar nerve in rodent lower incisor stem cells Reviewed

    Hayano S, Fukui Y, Kawanabe N, Nakamura M, Ishihara Y, Kamioka H

    Journal of Dental Research   97 ( 8 )   954 - 961   2018.7

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  • Physiological role of urothelial cancer-associated one long noncoding RNA in human skeletogenic cell differentiation. Reviewed International journal

    Takanori Ishikawa, Takashi Nishida, Mitsuaki Ono, Takeshi Takarada, Ha Thi Nguyen, Shinnosuke Kurihara, Takayuki Furumatsu, Yurika Murase, Masaharu Takigawa, Toshitaka Oohashi, Hiroshi Kamioka, Satoshi Kubota

    Journal of Cellular Physiology   233 ( 6 )   4825 - 4840   2018.6

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    A vast number of long-noncoding RNAs (lncRNA) are found expressed in human cells, which RNAs have been developed along with human evolution. However, the physiological functions of these lncRNAs remain mostly unknown. In the present study, we for the first time uncovered the fact that one of such lncRNAs plays a significant role in the differentiation of chondrocytes and, possibly, of osteoblasts differentiated from mesenchymal stem cells, which cells eventually construct the human skeleton. The urothelial cancer-associated 1 (UCA1) lncRNA is known to be associated with several human malignancies. Firstly, we confirmed that UCA1 was expressed in normal human chondrocytes, as well as in a human chondrocytic cell line; whereas it was not detected in human bone marrow mesenchymal stem cells (hBMSCs). Of note, although UCA1 expression was undetectable in hBMSCs, it was markedly induced along with the differentiation toward chondrocytes, suggesting its critical role in chondrogenesis. Consistent with this finding, silencing of the UCA1 gene significantly repressed the expression of chondrogenic genes in human chondrocytic cells. UCA1 gene silencing and hyper-expression also had a significant impact on the osteoblastic phenotype in a human cell line. Finally, forced expression of UCA1 in a murine chondrocyte precursor, which did not possess a UCA1 gene, overdrove its differentiation into chondrocytes. These results indicate a physiological and important role of this lncRNA in the skeletal development of humans, who require more sustained endochondral ossification and osteogenesis than do smaller vertebrates.

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  • The temporospatial pattern of energy metabolism coordinates the interactions between the bones and other organ systems Reviewed

    Ziyi Wang, Hiroshi Kamioka

    Journal of Oral Biosciences   60 ( 1 )   8 - 14   2018.3

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    Background: Bones adapt to loads by changing their structure. This biomechanical interaction and the formation/maintenance of bones are orchestrated by three major cell types residing in the bones: osteoblasts, osteocytes, and osteoclasts. Recent findings suggest that, in addition to their biomechanical interactions, bones and other organ systems may also communicate biochemically. Highlight: This brief review will discuss the interaction between the bones and the nervous system, vasculature, muscle, and fat tissues, with an emphasis on the role of the energy metabolism in these interactions. Conclusion: Studies on the connections between bones and other organ systems indicate the possible existence of a temporospatial pattern of energy metabolism through the cellular biorhythm and migration.

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  • 重度の埋伏永久歯を牽引誘導した2症例 Reviewed

    石川崇典, 星島光博, 川邉紀章, 上岡寛

    岡山歯学会雑誌   37 ( 1 )   9 - 13   2018.1

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  • A case of adult cleft palate patient treatment with differential maxillary lateral expand distraction osteogenesis using combined expansion appliances Reviewed

    Takeshi Yanagita, Hiroki Komori, Tomoyo Tanaka, Hiroshi Kamioka

    Orthodontic Waves   77   176 - 188   2018

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    A 34-year-old female patient who had undergone surgical treatment for a cleft in the soft palate during childhood received orthodontic treatment for crowding. She had a straight type facial profile and facial asymmetry with a concave area on the left side of the philtrum and mandibular deviation to the left. The surgical scar was observed on the center of the palate. She also had a severely constricted maxillary arch and unilateral cross bite on the left side. In this report, we suggest a novel method for uneven maxillary lateral expansion using a dento-osseous-supported expansion appliance in the frontal side of the maxilla and a modified dental-supported expansion appliance in the mid-palatal area. With this method, we achieved the optimal maxillary expansion in the maxillary frontal and molar areas. As a result of the surgically-assisted orthodontic treatment, facial asymmetry, the facial midline, and severe malocclusion were corrected. Furthermore, the resulting occlusion and facial symmetry were maintained over a 2-year retention period. Although attention must be paid regarding the retention of the expanded maxillary bone, our findings in the present study suggest that differential maxillary lateral expand distraction osteogenesis, which is performed using combined expansion appliances, can be successfully performed in patients with cleft palate.

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  • Three-dimensional morphometry of collagen fibrils in membranous bone. Reviewed International journal

    Mana Hashimoto, Noriyuki Nagaoka, Kaori Tabata, Tomoyo Tanaka, Ryuta Osumi, Naoya Odagaki, Toru Hara, Hiroshi Kamioka

    Integrative biology : quantitative biosciences from nano to macro   9 ( 11 )   868 - 875   2017.11

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    The collagen network acts as a scaffold for calcification and its three-dimensional structure influences bone strength. It is therefore important to observe the collagen network in detail and three-dimensionally. In this study, we observed the collagen network of chick embryonic calvariae in membranous bone three-dimensionally using orthogonally arranged FIB-SEM. A 25 × 25 μm area of chick embryonic calvaria was observed at a high resolution (25 nm per pixel). The inside of the bone (i.e. the primary calcified tissue), the bone cells (i.e. the osteoblasts and the osteocytes), the organelles, and the collagen fibrils were observed in detail. These structures were observed three-dimensionally using the Amira software program. In addition, the collagen fibrils of the bone were automatically extracted using the XTracing extension software program, and three-dimensional morphometry was performed. Almost all of the collagen fibrils ran along the longitudinal axis of the trabecular bone. We found that the regularity of the collagen fibril orientation was less remarkable in the osteoblast layer, which contained numerous osteoblasts. The collagen fibril orientation started to show regularity toward the central bone layer, which contained few bone cells.

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  • Synergistic acceleration of experimental tooth movement by supplementary high-frequency vibration applied with a static force in rats Reviewed

    Teruko Takano-Yamamoto, Kiyo Sasaki, Goudarzi Fatemeh, Tomohiro Fukunaga, Masahiro Seiryu, Takayoshi Daimaruya, Nobuo Takeshita, Hiroshi Kamioka, Taiji Adachi, Hiroto Ida, Atsushi Mayama

    SCIENTIFIC REPORTS   7 ( 1 )   241 - 249   2017.10

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    Several recent prospective clinical trials have investigated the effect of supplementary vibration applied with fixed appliances in an attempt to accelerate tooth movement and shorten the duration of orthodontic treatment. Among them, some studies reported an increase in the rate of tooth movement, but others did not. This technique is still controversial, and the underlying cellular and molecular mechanisms remain unclear. In the present study, we developed a new vibration device for a tooth movement model in rats, and investigated the efficacy and safety of the device when used with fixed appliances. The most effective level of supplementary vibration to accelerate tooth movement stimulated by a continuous static force was 3 gf at 70 Hz for 3 minutes once a week. Furthermore, at this optimum-magnitude, high-frequency vibration could synergistically enhance osteoclastogenesis and osteoclast function via NF-kappa B activation, leading to alveolar bone resorption and finally, accelerated tooth movement, but only when a static force was continuously applied to the teeth. These findings contribute to a better understanding of the mechanism by which optimum-magnitude high-frequency vibration accelerates tooth movement, and may lead to novel approaches for the safe and effective treatment of malocclusion.

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  • A Novel Method to Detect 3D Mandibular Changes Related to Soft-Diet Feeding Reviewed

    Kana Kono, Chihiro Tanikawa, Takeshi Yanagita, Hiroshi Kamioka, Takashi Yamashiro

    FRONTIERS IN PHYSIOLOGY   8 ( AUG )   64 - 79   2017.8

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    Craniofacial morphology varies among individuals, which is regulated by the interaction between genes and the environment. Soft-diet feeding is a widely-used experimental model for studying the association between the skeletal morphology and muscle-related loading on the bone. Traditionally, these studies have been based on linear and angular measurements provided on two-dimensional (2D) radiographs in the lateral view. However, 2D observation is based on simplification of the anatomical structures and cannot detect three-dimensional (3D) changes in detail. In this study, we newly developed a modified surface-based analysis with micro-3D computed tomography (CT) to examine and detect the 3D changes in the mandible associated with soft-diet feeding. Mice at 3 weeks of age were fed a powdered soft-diet (SD) or hard-diet (HD) of regular rodent pellets until 9 weeks of age. Micro-CT images were taken at age 9 weeks to reconstruct the anatomical architecture images. A computer-generated averaged mandible was superimposed to directly visualize the morphological phenotypes. Gross observation revealed the apparent changes at the posterior body of the mandible, the angular process and the condyle between HD and SD mice. Significant differences in the mapping indicated the regions of significant displacement in the SD mice over the averaged 3D image of the HD mice. This map revealed that vertical displacement was most evident in 3D changes. We also noted a combination of vertical, transverse and anteroposterior directions of displacement in the condylar growth, resulting in complicated shape changes in the whole condylar process in SD mice. In contrast, transverse displacement was more significant in the coronoid process. The map analysis further showed the significant outward displacement of the inner surface of the alveolar process, which consequently resulted in thinning of the alveolar process.

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  • CO2 low-level laser therapy has an early but not delayed pain effect during experimental tooth movement Reviewed

    T. Deguchi, D. G. Kim, H. Kamioka

    ORTHODONTICS & CRANIOFACIAL RESEARCH   20 ( 51 )   172 - 176   2017.6

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    Structured Abstract
    ObjectivesTo test the hypothesis that the use of low-level laser therapy (LLLT) reduces elevated pain by controlling the release of neurochemicals during orthodontic tooth movement.
    Setting and Sample PopulationDepartment of Orthodontics and Dentofacial Orthopedics, Okayama University. Sixty-five Sprague Dawley rats were subjected to tooth movement and LLLT.
    Materials and MethodsAdult Sprague Dawley rats were used in this study. Groups included day 0 controls, irradiation only controls and with or without irradiation sacrificed at 1, 3, 5, 7 and 14days after tooth movement (n=5 each, total n=65). Tooth movement was achieved by insertion of an elastic module between molar teeth. Immunohistochemistry for CD-11b, GFAP and c-fos in the brain stem was performed. Stains were quantified by constructing a three-dimensional image using IMARIS, and counted using NEURON TRACER and WinROOF software. Two-way ANOVA followed by a Tukey's post hoc test (P&lt;.05) was used for statistical comparison between groups.
    ResultsC-fos expression was significantly increased at one and three days after tooth movement. LLLT significantly diminished this increase in c-fos expression only at one day after tooth movement CD-b11 and GFAP expression also significantly increased after tooth movement. No significant change was observed for CD-11b and GFAP expression in the central nervous system upon LLLT.
    ConclusionLow-level laser therapy may reduce early neurochemical markers but have no effect on delayed pain neurochemical markers after tooth movement.

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  • Comparative evaluation of treatment outcomes between temporary anchorage devices and Class III elastics in Class III malocclusions Reviewed

    Masahiro Nakamura, Noriaki Kawanabe, Tomoki Kataoka, Takashi Murakami, Takashi Yamashiro, Hiroshi Kamioka

    AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS   151 ( 6 )   1116 - 1124   2017.6

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    Introduction: Our objective was to elucidate the differences in treatment outcomes caused by the different mechanics of temporary anchorage devices (TADs) and Class III elastics in patients with Class III malocclusions. Methods: Records of 23 patients with Angle Class III malocclusion were selected retrospectively. All had been treated with nonextraction comprehensive orthodontic treatment; 11 were treated with TADs and 12 with Class III elastics. Pretreatment and posttreatment lateral cephalograms were used for evaluation of the treatment outcomes. A paired t test and a Student t test were used for statistical analysis. Results: In both groups, proper overjet and Class I molar relationships were achieved, and the occlusal plane was rotated counterclockwise. In the elastics group, distal tipping of the mandibular molars, extrusion of the mandibular incisors and maxillary molars, clockwise rotation of the mandibular plane angle, and increased ANB angle were observed. In the TADs group, distal tipping and intrusion of the mandibular molars, bodily movement of the mandibular incisors, and reduced mandibular plane angle were observed. Conclusions: In nonextraction treatment for Class III malocclusions, the mandibular plane angle was increased in the elastics group, whereas it was decreased in TADs group. Thus, we suggest that Class III elastics are preferred for low-angle, short-face patients, whereas TADs are preferred for high-angle, long-face patients.

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  • Orthognathic surgery during breast cancer treatment-A case report. International journal

    Tsuyoshi Shimo, Norie Yoshioka, Masahiro Nakamura, Soichiro Ibaragi, Tatsuo Okui, Yuki Kunisada, Masanori Masui, Mayumi Yao, Koji Kishimoto, Shoko Yoshida, Akiyoshi Nishiyama, Hiroshi Kamioka, Akira Sasaki

    International journal of surgery case reports   31   30 - 34   2017

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    INTRODUCTION: In recent years, patients with orthognathic surgery in middle-aged and elderly people have come to be a more frequent occurrence. Breast cancer is the most frequently diagnosed cancer in woman worldwide, and its prevalence rate is steadily increasing. PRESENTATION OF CASE: We report a case of a 47-year-old Japanese woman in whom left-side breast cancer (Stage 1) was unexpectedly found just before orthognathic surgery in April 2012. Breast-conserving surgery was performed (estrogen receptor+, progesterone receptor+, HER2 -, surgical margin+, sentinel lymph node +) that May. From June to August docetaxel (75mg/m2) and cyclophosphamide (600mg/m2) were administrated four times every 21days and thereafter radiotherapy (total 60Gy) was completed. The cancer surgeon declared the prognosis good and the patient had a strong desire to undergo orthognathic surgery, so in November we performed a bimaxillary osteotomy, and administration of tamoxifen began 6 weeks after the osteotomy. DISCUSSION: There are breast cancer cases in which the prognosis is sufficiently good for a planned orthognathic surgery to proceed. Good communication among surgeons and the patient is important. CONCLUSION: We experienced a case in which breast cancer was found just before the orthognathic surgery; we performed a bimaxillary osteotomy, including follow-up tamoxifen administration, during breast cancer treatment.

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  • 岡山大学大学院歯科矯正学分野研究室における過去15年間および2013年から2016年の4年間の岡山大学の学生および職員に対する矯正相談の実態調査 Reviewed

    田中智代, 村上隆, 石原嘉人, 中村政裕, 片岡伴記, 植田紘貴, 早野暁, 星島光博, 川邉紀章, 上岡寛

    岡山歯学会雑誌   36 ( 2 )   45 - 51   2017

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  • 歯科矯正用アンカースクリューを用いて多数埋伏歯の同時牽引を行った鎖骨頭蓋異骨症患者の1例 Reviewed

    石本和也, 川邉紀章, Ei Hsu, Hlaing Ei, 片岡伴記, 村上隆, 上岡寛

    岡山歯学会雑誌   36 ( 1 )   7 - 12   2017

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  • The different effects of intraoral vertical ramus osteotomy (IVRO) and sagittal split ramus osteotomy (SSRO) on mandibular border movement Reviewed

    Hiroki Komori, Noriaki Kawanabe, Tomoki Kataoka, Yui Kato, Atsuro Fujisawa, Takashi Yamashiro, Hiroshi Kamioka

    Cranio - Journal of Craniomandibular Practice   36 ( 4 )   1 - 6   2017

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    © 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group. Objectives: This study investigated the different effects of intraoral vertical ramus osteotomy (IVRO) and sagittal split ramus osteotomy (SSRO) on mandibular border movement. Methods: The participants included 22 patients receiving IVRO and 22 patients receiving SSRO who were treated at Okayama University Hospital. Their mandibular border movement was evaluated in three dimensions with 6° of freedom using an optical recording system. Results: A strong correlation between condylar and lower incisor movement was observed during maximum jaw protrusion and laterotrusion. Significant improvements in condylar and lower incisor movement were detected after orthognathic surgery during maximum jaw protrusion and laterotrusion in the IVRO group and during maximum jaw protrusion in the SSRO group. Discussion: IVRO likely achieves greater improvement in jaw movement than SSRO. Therefore, the application of IVRO could be considered in the treatment of patients with jaw deformities featuring temporomandibular joint problems.

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  • 岡山大学歯科矯正学分野「歯の移動の臨床手技実習」における60分授業・クォーター制への対応 Reviewed

    片岡伴記, 星島光博, 原規子, 中村政裕, 早野暁, 村上隆, 川邊紀章, 上岡寛

    岡山歯学会雑誌   35   59 - 65   2017

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  • 下顎の垂直的な変化を制御するために歯科矯正用アンカースクリューとスライディングメカニクスを用い上下顎両側第二小臼歯を抜去して治療を行った上下顎前突症例 Reviewed

    川邉紀章, 河野加奈, 片岡伴記, 中村政裕, 上岡寛

    中・四国矯正歯科学会雑誌   29 ( 1 )   27 - 36   2017

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  • 骨格性下顎前突患者に対して上顎両側側切歯欠損部に下顎両側側切歯を移植し,カモフラージュ治療を行った一症例

    片岡伴記, 藤澤厚郎, 川邉紀章, 上岡寛

    中・四国矯正歯科学会雑誌   29 ( 1 )   17 - 26   2017

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  • Modification of dentofacial growth associated with Goldenhar syndrome Reviewed

    Ei Ei Hsu Hlaing, Yoshihito Ishihara, Chikako Hara, Hiroshi Kamioka

    Acta Medica Okayama   71 ( 5 )   437 - 443   2017

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    The rare developmental defect, Goldenhar syndrome is characterized by complex craniofacial and dentofacial anomalies. Here we describe the successful orthodontic treatment of a 5-year-old Japanese Goldenhar syndrome patient with mild facial asymmetry, right microtia, right-side hearing loss, and tongue-thrusting by a modification of dentofacial growth using a non-surgical orthopedic treatment approach. Improvement of the vertical discrepancies on the affected side and canted occlusal plane as well as mandibular deviation were achieved with a functional orthopaedic approach. Stable and acceptable occlusion were obtained over the 32-month post-retention period. A non-surgical orthodontic treatment approach offers satisfactory facial aesthetic outcomes in Goldenhar syndrome.

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  • Involvement of multiple CCN family members in platelets that support regeneration of joint tissues Reviewed

    Chikako Hara, Satoshi Kubota, Takashi Nishida, Miki Hiasa, Takako Hattori, Eriko Aoyama, Yoshinori Moriyama, Hiroshi Kamioka, Masaharu Takigawa

    Modern Rheumatology   26 ( 6 )   940 - 949   2016.11

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    Objectives: Platelet-rich plasma (PRP) has been widely used to enhance the regeneration of damaged joint tissues, such as osteoarthritic and rheumatoid arthritic cartilage. The aim of this study is to clarify the involvement of all of the CCN family proteins that are crucially associated with joint tissue regeneration. Methods: Cyr61-CTGF-NOV (CCN) family proteins in human platelets and megakaryocytic cells were comprehensively analyzed by Western blotting analysis. Production of CCN family proteins in megakaryocytes in vivo was confirmed by immunofluorescence analysis of mouse bone marrow cells. Effects of CCN family proteins found in platelets on chondrocytes were evaluated by using human chondrocytic HCS-2/8 cells. Results: Inclusion of CCN2, a mesenchymal tissue regenerator, was confirmed. Of note, CCN3, which counteracts CCN2, was newly found to be encapsulated in platelets. Interestingly, these two family members were not detectable in megakaryocytic cells, but their external origins were suggested. Furthermore, we found for the first time CCN5 and CCN1 that inhibits ADAMTS4 in both platelets and megakaryocytes. Finally, application of a CCN family cocktail mimicking platelets onto HCS-2/8 cells enhanced their chondrocytic phenotype. Conclusions: Multiple inclusion of CCN1, 2 and 3 in platelets was clarified, which supports the harmonized regenerative potential of PRP in joint therapeutics.

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  • A case of severe mandibular retrognathism with bilateral condylar deformities treated with Le Fort I osteotomy and two advancement genioplasty procedures Reviewed

    Masahiro Nakamura, Takeshi Yanagita, Tatsushi Matsumura, Takashi Yamashiro, Seiji Iida, Hiroshi Kamioka

    Korean Journal of Orthodontics   46 ( 6 )   395 - 408   2016.11

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    We report a case involving a young female patient with severe mandibular retrognathism accompanied by mandibular condylar deformity that was effectively treated with Le Fort I osteotomy and two genioplasty procedures. At 9 years and 9 months of age, she was diagnosed with Angle Class III malocclusion, a skeletal Class II jaw relationship, an anterior crossbite, congenital absence of some teeth, and a left-sided cleft lip and palate. Although the anterior crossbite and narrow maxillary arch were corrected by interceptive orthodontic treatment, severe mandibular hypogrowth resulted in unexpectedly severe mandibular retrognathism after growth completion. Moreover, bilateral condylar deformities were observed, and we suspected progressive condylar resorption (PCR). There was a high risk of further condylar resorption with mandibular advancement surgery
    therefore, Le Fort I osteotomy with two genioplasty procedures was performed to achieve counterclockwise rotation of the mandible and avoid ingravescence of the condylar deformities. The total duration of active treatment was 42 months. The maxilla was impacted by 7.0 mm and 5.0 mm in the incisor and molar regions, respectively, while the pogonion was advanced by 18.0 mm. This significantly resolved both skeletal disharmony and malocclusion. Furthermore, the hyoid bone was advanced, the pharyngeal airway space was increased, and the morphology of the mandibular condyle was maintained. At the 30-month follow-up examination, the patient exhibited a satisfactory facial profile. The findings from our case suggest that severe mandibular retrognathism with condylar deformities can be effectively treated without surgical mandibular advancement, thus decreasing the risk of PCR.

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  • Alternation in the gap-junctional intercellular communication capacity during the maturation of osteocytes in the embryonic chick calvaria. Reviewed International journal

    Ziyi Wang, Naoya Odagaki, Tomoyo Tanaka, Mana Hashimoto, Masahiro Nakamura, Satoru Hayano, Yoshihito Ishihara, Noriaki Kawanabe, Hiroshi Kamioka

    Bone   91   20 - 9   2016.10

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    INTRODUCTION: The intercellular network of cell-cell communication among osteocytes is mediated by gap junctions. Gap junctional intercellular communication (GJIC) is thought to play an important role in the integration and synchronization of bone remodeling. To further understand the mechanism of bone development it is important to quantify the difference in the GJIC capacity of young and developmentally mature osteocytes. MATERIALS AND METHODS: We first established an embryonic chick calvaria growth model to show the growth of the calvaria in embryos at 13 to 21days of age. We then applied a fluorescence recovery after photobleaching (FRAP) technique to compare the difference in the GJIC capacity of young osteocytes with that of developmentally mature osteocytes. Finally, we quantified the dye (Calcein) diffusion from the FRAP data using a mathematic model of simple diffusion which was also used to identify simple diffusion GJIC pattern cells (fitted model) and accelerated diffusion GJIC pattern cells (non-fitted model). RESULTS: The relationship between the longest medial-lateral length of the calvaria (frontal bone) and the embryonic age fit a logarithmic growth model: length=5.144×ln(day)-11.340. The morphometric data during osteocyte differentiation showed that the cellular body becomes more spindle-shaped and that the cell body volume decreased by approximately 22% with an increase in the length of the processes between the cells. However, there were no significant differences in the cellular body surface area or in the distance between the mass centres of the cells. The dye-displacement rate in young osteocytes was significantly higher than that in developmentally mature osteocytes: dye displacement only occurred in 26.88% of the developmentally mature osteocytes, while it occurred in 64.38% of the young osteocytes. Additionally, in all recovered osteocytes, 36% of the developmentally mature osteocytes comprised non-fitted model cells while 53.19% of the young osteocytes were the non-fitted model, which indicates the active transduction of dye molecules. However, there were no statistically significant differences between the young and developmentally mature osteocytes with regard to the diffusion coefficient, permeability coefficient, or permeance of the osteocyte processes, which were 3.93±3.77 (×10(-8)cm(2)/s), 5.12±4.56 (×10(-5)cm(2)/s) and 2.99±2.47 (×10(-13)cm(2)/s) (mean±SD), respectively. CONCLUSIONS: These experiments comprehensively quantified the GJIC capacity in the embryonic chick calvaria and indicated that the cell-cell communication capacity of the osteocytes in the embryonic chick calvaria was related to their development.

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  • Collagen production of osteoblasts revealed by ultra-high voltage electron microscopy Reviewed

    Rumiko Hosaki-Takamiya, Mana Hashimoto, Yuichi Imai, Tomoki Nishida, Naoko Yamada, Hirotaro Mori, Tomoyo Tanaka, Noriaki Kawanabe, Takashi Yamashiro, Hiroshi Kamioka

    Journal of Bone and Mineral Metabolism   34 ( 5 )   491 - 499   2016.9

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    In the bone, collagen fibrils form a lamellar structure called the “twisted plywood-like model.” Because of this unique structure, bone can withstand various mechanical stresses. However, the formation of this structure has not been elucidated because of the difficulty of observing the collagen fibril production of the osteoblasts via currently available methods. This is because the formation occurs in the very limited space between the osteoblast layer and bone matrix. In this study, we used ultra-high-voltage electron microscopy (UHVEM) to observe collagen fibril production three-dimensionally. UHVEM has 3-MV acceleration voltage and enables us to use thicker sections. We observed collagen fibrils that were beneath the cell membrane of osteoblasts elongated to the outside of the cell. We also observed that osteoblasts produced collagen fibrils with polarity. By using AVIZO software, we observed collagen fibrils produced by osteoblasts along the contour of the osteoblasts toward the bone matrix area. Immediately after being released from the cell, the fibrils run randomly and sparsely. But as they recede from the osteoblast, the fibrils began to run parallel to the definite direction and became thick, and we observed a periodical stripe at that area. Furthermore, we also observed membrane structures wrapped around filamentous structures inside the osteoblasts. The filamentous structures had densities similar to the collagen fibrils and a columnar form and diameter. Our results suggested that collagen fibrils run parallel and thickly, which may be related to the lateral movement of the osteoblasts. UHVEM is a powerful tool for observing collagen fibril production.

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  • Antero-posterior and vertical facial type variations influence the aesthetic preference of the antero-posterior lip positions Reviewed

    Takashi Murakami, Tomoki Kataoka, Junpei Tagawa, Takashi Yamashiro, Hiroshi Kamioka

    European Journal of Orthodontics   38 ( 4 )   414 - 421   2016.8

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    Objectives: The purposes of this study were to investigate whether the antero-posterior and vertical facial type variations influenced the favoured lip positions and to elucidate whether the favoured lip positions differed between orthodontists and laypersons. Methods: An average profile was constructed from a Japanese female with Class I occlusion and a well-balanced profile, as assessed by several cephalometric analyses. Nine facial types were composed by morphing the chin antero-posteriorly and/or vertically. Thirteen morphed lip profiles were constructed by moving the lips antero-posteriorly in the nine facial types, respectively. Seventy-seven Japanese laypersons and 30 orthodontists were asked to choose the top three most-favoured lip positions for each facial type. Results: The protruded lip positions were significantly favoured for all the mandibular protrusive facial types. The retruded lip positions were significantly favoured for the short facial type of the antero-posteriorly average and mandibular retrusive-short facial types. Among the mandibular protrusive facial types, the protruded lip positions were significantly favoured for the long facial type. The retruded lip positions were statistically more attractive for orthodontists than laypersons on the antero-posteriorly average-short, mandibular protrusive-short, mandibular retrusive-vertically average, antero-posteriorly average-vertically average, and mandibular retrusive-long facial types. Limitations: Our data were limited by the specific ethnic groups and variety of facial types. Conclusion: The favoured antero-posterior lip position was affected by not only the antero-posterior facial disproportion but also by the vertical dimensions. The favoured lip positions differed between orthodontists and laypersons. These results might be helpful in deciding between extraction and non-extraction treatment in borderline cases.

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  • Orthodontic treatment of a patient with unilateral orofacial muscle dysfunction: The efficacy of myofunctional therapy on the treatment outcome Reviewed

    Yasuyo Sugawara, Yoshihito Ishihara, Teruko Takano-Yamamoto, Takashi Yamashiro, Hiroshi Kamioka

    AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS   150 ( 1 )   167 - 180   2016.7

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    The orofacial muscle is an important factor in the harmony of the occlusion, and its dysfunction significantly influences a patient's occlusion after craniofacial growth and development. In this case report, we describe the successful orthodontic treatment of a patient with unilateral orofacial muscle dysfunction. A boy, 10 years 0 months of age, with a chief complaint of anterior open bite, was diagnosed with a Class III malocclusion with facial musculoskeletal asymmetry. His maxillomandibular relationships were unstable, and he was unable to lift the right corner of his mouth upon smiling because of weak right orofacial muscles. A satisfactory occlusion and a balanced smile were achieved after orthodontic treatment combined with orofacial myofunctional therapy, including muscle exercises. An acceptable occlusion and facial proportion were maintained after a 2-year retention period. These results suggest that orthodontic treatment with orofacial myofunctional therapy is an effective option for a patient with orofacial muscle dysfunction.

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    Other Link: http://orcid.org/0000-0002-4419-9643

  • Effect of minocycline on induced glial activation by experimental tooth movement Reviewed

    Toru Deguchi, Rie Adachi, Hiroshi Kamioka, Do-Gyoon Kim, Henry W. Fields, Teruko Takano-Yamamoto, Hiroyuki Ichikawa, Takashi Yamashiro

    American Journal of Orthodontics and Dentofacial Orthopedics   149 ( 6 )   881 - 888   2016.6

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    Introduction Orthodontic tooth movement causes pain to a patient. Glial cells are nonneuronal cells in the central nervous system and are implicated in various types of pain. In this study, we assessed glial activation responses after experimental tooth movement using immunocytochemical detection of anti-CD11b (OX42) and glial fibrillary acidic protein immunoreactivity to illustrate the microglial and astrocytes response, respectively. In addition, the effect of minocycline in reducing pain during tooth movement was also investigated. Methods Fifty-five Sprague Dawley rats with and without administration of minocycline after 1, 3, 5, 7, and 14 days (n = 5, for each) of tooth movement were used. Immunohistochemistry for microglia (OX42) and astrocyte (glial fibrillary acidic protein) were performed at the medullary dorsal horn (trigeminal subnucleus caudalis). Three-dimensional quantitative analysis was performed with a confocal fluorescence microscope and a software program. Results There was a significant increase in the OX42 and glial fibrillary acidic protein immunoreactivity in response to tooth movement in the medullary dorsal horn. Furthermore, systematic administration of minocycline, a selective inhibitor of microglial activation, significantly attenuated the nociceptive c-Fos expression in the medullary dorsal horn that was induced by experimental tooth movement. Conclusions These data indicate the possible importance of microglial activation in the development of orthodontic pain. This is also the first report on the systematic application of minocycline.

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  • A single-center, open-label, randomized controlled clinical trial to evaluate the efficacy and safety of the indirect bonding technique Reviewed

    Takashi Murakami, Noriaki Kawanabe, Tomoki Kataoka, Mitsuhiro Hoshijima, Hiroki Komori, Atsuro Fujisawa, Hiroshi Kamioka

    Acta Medica Okayama   70 ( 5 )   413 - 416   2016

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    Although accurate bracket placement is essential for orthodontic treatment, many practitioners apply brackets indiscriminately with direct or indirect bonding techniques. Nonetheless, there have been few prospective clinical comparisons of the 2 techniques. We will therefore conduct a single-center, randomized control trial in 100 patients aged ≥2 years and diagnosed with malocclusion. All patients will receive orthodontic treatment using brackets with direct or indirect bonding techniques. The primary endpoints will be the total treatment time, occlusal index, discomfort at bonding, and oral hygiene after bonding. This study will clarify whether indirect bonding can improve the efficiency of orthodontic treatment.

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  • Orthodontic treatment of a patient with bilateral congenitally missing maxillary canines: The effects of first premolar substitution on the functional outcome Reviewed

    Kumi Sumiyoshi, Yoshihito Ishihara, Hiroki Komori, Takashi Yamashiro, Hiroshi Kamioka

    Acta Medica Okayama   70 ( 1 )   57 - 62   2016

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    Permanent canines are thought to play a pivotal role in obtaining an ideal occlusion. Dentists occasionally encounter patients who lack canines and are therefore missing a key to harmonious guidance during functional mandibular excursions. This case report describes the substitution of maxillary first premolars for congenitally missing canines in the context of an orthodontic treatment plan. A boy, age 10 years and 11 months, with a chief complaint of crooked teeth was diagnosed with Class II division 2 malocclusion associated with a high mandibular plane angle and deep overbite. A stable occlusion with a satisfactory facial profile and functional excursions without interference were achieved after a comprehensive two-stage orthodontic treatment process. The resulting occlusion and satisfactory facial profile were maintained for 12 months. These results indicate that substituting the first premolars for the canines is an effective option in treating patients with missing canines while maintaining functional goals.

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  • 歯科矯正用アンカースクリューおよびオクルーザルスプリントを 用いて咬合再構築を行った成人開咬症例 Reviewed

    早野暁, 星島光博, 石川崇典, 上岡寛

    中・四矯歯誌   28 ( 1 )   1 - 9   2016

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  • A survey of patient's attitudes and over all satisfaction for orthodontic treatment at Okayama University Hospital Orthodontic Clinic Reviewed

    The journal of Okayama Dental Society   34 ( 2 )   33 - 45   2015.12

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  • 下顎両側第一大臼歯抜歯空隙への下顎第二大臼歯および埋伏第三大臼歯の近心移動を行った前歯部開咬症例

    中村 政裕, 川邉 紀章, 山城 隆, 上岡 寛

    中・四国矯正歯科学会雑誌   27 ( 1 )   13 - 21   2015.8

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    患者は20歳6ヵ月の女性で、上顎前歯部の叢生および前歯部開咬を主訴に岡山大学病院矯正歯科を受診した。側貌は凸型、オトガイの後退感、口唇閉鎖不全が認められた。両側アングルI級、前歯部開咬を呈し、上下顎前歯はやや唇側傾斜していた。さらに、予後不良な下顎左側第一大臼歯、上下顎歯列の叢生、軽度の咬合平面の二面性が認められた。分析の結果、上下顎前歯部叢生を伴う骨格性II級、ハイアングル、ロングフェイス、前歯部開咬症例と診断された。予後不良歯である下顎左側第一大臼歯に加え下顎右側第一大臼歯及び上顎両側第一小臼歯の抜歯を伴うカモフラージュ治療を行うこととなった。下顎両側第二・第三大臼歯の近心移動およびスピー彎曲の改善に伴い下顎臼歯の挺出が生じ、下顎骨の時計回り回転が危惧されたため、下顎骨回転に伴う側貌の悪化を防ぐことを目的に上顎両側大臼歯の圧下を行うこととした。動的治療期間は4年5ヵ月であった。オトガイの後退感は残るものの口元の突出感は改善し良好な側貌を獲得した。治療前後の重ね合わせでは、上下顎前歯歯軸および臼歯移動量は治療目標設定値と近似しており、下顎下縁平面角は維持されていた。保定2年が経過したが、咬合は安定している。長期予後不良な第一大臼歯を認める場合、下顎第一大臼歯抜歯による下顎第二・第三大臼歯の近心移動は有用な治療法であるが、ハイアングル・ロングフェイス症例においては、下顎大臼歯の挺出による顔貌の悪化を防ぐため上顎大臼歯の圧下を行うことが望ましいと考える。(著者抄録)

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  • Interdisciplinary orthodontic treatment for a patient with generalized aggressive periodontitis: Assessment of IgG antibodies to identify type of periodontitis and correct timing of treatment

    Yoshihito Ishihara, Kazuya Tomikawa, Toru Deguchi, Tadashi Honjo, Koji Suzuki, Takayuki Kono, Takuo Kuboki, Hiroshi Kamioka, Shogo Takashiba, Takashi Yamashiroi

    AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS   147 ( 6 )   766 - 780   2015.6

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    Aggressive periodontitis is a great challenge to clinicians when providing orthodontic treatment because of the potential for progression of periodontal disease. In this article, we report the successful comprehensive orthodontic treatment of bimaxillary protrusion and severe crowding in an adult with generalized aggressive periodontitis. A woman, aged 22 years 7 months, with a chief complaint of incisal crowding was diagnosed with a skeletal Class I malocclusion associated with severe anterior crowding, possibly worsened by generalized aggressive periodontitis. In addition to a periodontal examination, a blood IgG antibody titer analysis and microbiologic examination for periodontal pathogens were used to diagnose the type of periodontal disease and determine the proper timing to initiate orthodontic treatment. The total active treatment period was 28 months, followed by periodontal prostheses and regeneration therapy. Consequently, satisfactory facial profile, occlusion, and periodontal health were maintained for at least 36 months. These results indicate that efficient screening is important for providing successful orthodontic treatment in patients with advanced periodontal disease. This report also demonstrates the diagnostic importance of blood IgG antibody titer assays and microbiologic examinations to detect periodontal pathogens.

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  • Maxillary Advancement for Unilateral Crossbite in a Patient with Sleep Apnea Syndrome

    Mitsuhiro Hoshijima, Tadashi Honjo, Norifumi Moritani, Seiji Iida, Takashi Yamashiro, Hiroshi Kamioka

    ACTA MEDICA OKAYAMA   69 ( 3 )   177 - 182   2015.6

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    This article reports the case of a 44-year-old male with skeletal Class III, Angle Class III malocclusion and unilateral crossbite with concerns about obstructive sleep apnea syndrome (OSAS), esthetics and functional problems. To correct the skeletal deformities, the maxilla was anteriorly repositioned by employing LeFort I osteotomy following pre-surgical orthodontic treatment, because a mandibular setback might induce disordered breathing and cause OSAS. After active treatment for 13 months, satisfactory occlusion was achieved and an acceptable facial and oral profile was obtained. In addition, the apnea hypopnea index (AHI) decreased from 18.8 preoperatively to 10.6 postoperatively. Furthermore, after a follow-up period of 7 months, the AHI again significantly decreased from 10.6 to 6.2. In conclusion, surgical advancement of the maxilla using LeFort I osteotomy has proven to be useful in patients with this kind of skeletal malocclusion, while preventing a worsening of the OSAS.

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  • Osteocyte bioimaging

    Hiroshi Kamioka

    Journal of Oral Biosciences   57 ( 2 )   61 - 64   2015.5

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    Background Newly developed visualization methods often lead to breakthroughs in the bioscience field. In particular, the ability to reveal temporal-spatial responses in cells, while visualizing molecular events through bioimaging techniques is very important. One such event is the regulation of bone remodeling by osteocytes. It is thought that osteocyte processes sense the flow of interstitial fluid that is driven through the osteocyte canaliculi by mechanical stimuli caused in the bone. However, the precise mechanism by which the flow elicits a cellular response is still unknown. Highlight It is critical to obtain precise morphological and/or morphometrical data from osteocytes and their surrounding microenvironment. In this review, we describe our application of confocal laser scanning microscopy to visualize osteocyte morphology in the bone and the combination of ultra-high voltage electron microscopy (UHVEM) and computer simulation of fluid flow to reveal the mechanosensitivity of osteocytes in the bone. Conclusion The osteocyte network in the bone as well as the microstructure of osteocyte cell processes and the surrounding bone matrix were visualized. We found fluorescence to be useful for studying the osteocyte network morphology. Additionally, the combination of UHVEM and computer simulation is a powerful tool to study the fluid flow in osteocyte canaliculi.

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  • Topical Application of Lithium Chloride on the Pulp Induces Dentin Regeneration Reviewed

    Kazuya Ishimoto, Satoru Hayano, Takeshi Yanagita, Hiroshi Kurosaka, Noriaki Kawanabe, Shinsuke Itoh, Mitsuaki Ono, Takuo Kuboki, Hiroshi Kamioka, Takashi Yamashiro

    PLOS ONE   10 ( 3 )   e0121938   2015.3

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    We herein describe a novel procedure for dentin regeneration that mimics the biological processes of tooth development in nature. The canonical Wnt signaling pathway is an important regulator of the Dentin sialophosphoprotein (Dspp) expression. Our approach mimics the biological processes underlying tooth development in nature and focuses on the activation of canonical Wnt signaling to trigger the natural process of dentinogenesis. The coronal portion of the dentin and the underlying pulp was removed from the first molars. We applied lithium chloride (LiCl), an activator of canonical Wnt signaling, on the amputated pulp surface to achieve transdifferentiation toward odontoblasts from the surrounding pulpal cells. MicroCT and microscopic analyses demonstrated that the topical application of LiCl induced dentin repair, including the formation of a complete dentin bridge. LiCl-induced dentin is a tubular dentin in which the pulp cells are not embedded within the matrix, as in primary dentin. In contrast, a dentin bridge was not induced in the control group treated with pulp capping with material carriers alone, although osteodentin without tubular formation was induced at a comparatively deeper position from the pulp exposure site. We also evaluated the influence of LiCl on differentiation toward odontoblasts in vitro. In the mDP odontoblast cell line, LiCl activated the mRNA expression of Dspp, Axin2 and Kallikrein 4 (Klk4) and downregulated the Osteopontin (Osp) expression. These results provide a scientific basis for the biomimetic regeneration of dentin using LiCl as a new capping material to activate dentine regeneration.

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  • Runx/Cbfb signaling regulates postnatal development of granular convoluted tubule in the mouse submandibular gland. International journal

    Md Nurul Islam, Shinsuke Itoh, Takeshi Yanagita, Kumi Sumiyoshi, Satoru Hayano, Koh-Ichi Kuremoto, Hiroshi Kurosaka, Tadashi Honjo, Noriaki Kawanabe, Hiroshi Kamioka, Takayoshi Sakai, Naozumi Ishimaru, Ichiro Taniuchi, Takashi Yamashiro

    Developmental dynamics : an official publication of the American Association of Anatomists   244 ( 3 )   488 - 96   2015.3

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    BACKGROUND: The rodent salivary gland is not fully developed at birth and the cellular definitive differentiation takes place postnatally. However, little is known about its molecular mechanism. RESULTS: Here we provide the loss-of-function genetic evidence that Runx signaling affects postnatal development of the submandibular gland (SMG). Core binding factor β (Cbfb) is a cotranscription factor which forms a heterodimer with Runx proteins. Cbfb was specifically expressed in the duct epithelium, specifically in the SMG. Epithelial Cbfb deficiency resulted in decrease in the size of the SMG and in the saliva secretion on postnatal day 35. The Cbfb mutant SMG specifically exhibited involution of the granular convoluted tubules (GCT), with a down-regulated expression of its marker genes, such as Klk1, Ngf, and Egf. The induction of GCT is under the control of androgens, and the Cbfb mutant SMG demonstrated down-regulated expression of Crisp3, an androgen-dependent transcript. Because the circulating testosterone or tissue dihydrotestosterone levels were not affected in the Cbfb mutants, it appears that Runx/Cbfb signaling regulate androgen receptor pathway, but does not affect the circulating testosterone levels or the enzymatic conversion to DHT. CONCLUSIONS: Runx signaling is important in the postnatal development of androgen-dependent GCT in the SMG.

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  • 歯科矯正用アンカースクリューを用いて下顎前歯の圧下により過蓋咬合を治療したAngle II級成人症例

    福島 宏明, 本城 正, 片岡 伴記, 川邉 紀章, 山城 隆, 上岡 寛

    中・四国矯正歯科学会雑誌   26 ( 1 )   41 - 48   2014.8

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    患者は初診時年齢37歳9ヵ月の女性で、口唇の突出感を主訴に岡山大学病院矯正歯科を受診した。側貌はConvex typeで、下顎の後退感、上下口唇の突出、口唇閉鎖時のオトガイ部の筋緊張および口唇閉鎖不全を認めた。口腔内はAngle II級であり、オーバージェットが大きかった。また、下顎前歯が挺出しており、過大なSpee彎曲を認め、過蓋咬合を呈していた。さらに、上顎前歯は舌側傾斜し、下顎歯列に叢生を認めた。骨格的には骨格性II級、high mandibular plane angleであった。分析の結果、上顎前歯の舌側傾斜を伴う下顎骨の後退による骨格性II級、Angle II級、high mandibular plane angle、過蓋咬合症例と診断した。上下顎両側第一小臼歯の抜歯を伴うカモフラージュ治療を行うことになったが、過蓋咬合を治療する従来のメカニクスでは、小臼歯の挺出に伴う下顎下縁平面のさらなる開大が危惧された。そのため、下顎前歯圧下のための固定源として、下顎両側側切歯・犬歯間頬側歯槽部の歯科矯正用アンカースクリューを利用した。また、上顎前歯を舌側へ歯体移動させるための固定源として、上顎両側第一大臼歯・第二大臼歯間および上顎両側側切歯・犬歯間頬側歯槽部の歯科矯正用アンカースクリューを利用した。治療の結果、下顎の時計回りの回転を起こさずに下顎前歯は圧下、上顎前歯はほぼ舌側へ歯体移動し、良好な咬合関係を確立できた。(著者抄録)

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  • 審美的に好ましい上唇-下唇-オトガイの前後的位置関係に関するアンケート調査

    村上 隆, 片岡 伴記, 川邉 紀章, 山城 隆, 上岡 寛

    中・四国矯正歯科学会雑誌   26 ( 1 )   19 - 24   2014.8

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    【目的】好ましい側貌の基準を具体的・客観的に明らかにすることは、実際の矯正歯科治療における治療ゴールを設定する上で必要不可欠である。本研究は、一般人を対象にアンケートを行い、上唇、下唇およびオトガイの前後的位置関係が与える側貌の審美的認識への影響について検討を行った。【資料および方法】標準的な日本人女性の側貌を元に、専用ソフトウェアを用いて下顎骨を前後に10mmずつ移動させ、上顎前突、標準および下顎前突の3パターンの側貌シルエットを作成した。次に、その上唇と下唇をそれぞれ3mmずつ前後に移動させ、17通りの口唇位を作成した。一般人178名を対象に、3パターンの側貌それぞれに17通りの口唇位を組み合わせたシルエットを提示し、好ましい側貌に関するアンケートを実施した。【結果および考察】標準的な側貌では、上唇-下唇の前後的距離の差が+3mmの側貌が最も好まれた。口唇とオトガイとの前後的距離の差が大きい上顎前突の側貌では、上唇-下唇の前後的距離が比較的大きい+6mmの側貌が最も好まれた。口唇とオトガイとの前後的距離の差が小さい下顎前突の側貌では、上唇-下唇の前後的距離の差により好まれ方に違いは認められなかった。【結論】骨格の違いにより、審美的に好ましい上唇-下唇の前後的距離には差異が存在することが明らかになった。審美的に好ましい上下口唇およびオトガイの前後的位置は、上唇-下唇-オトガイの前後的距離の比により規定される可能性が示唆された。(著者抄録)

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  • 蛍光ライブイメージングを用いた骨組織中における細胞内カルシウムオシレーションの計測

    石原 嘉人, 菅原 康代, 上岡 寛, 川邉 紀章, 成瀬 恵治, 山城 隆

    日本骨形態計測学会雑誌   24 ( 1 )   15 - 22   2014.3

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    細胞内カルシウム(Ca2+)は多様な細胞機能を仲介する重要な情報伝達物質であり、Ca2+オシレーションと呼ばれる周期的なCa2+濃度の上昇様式は、骨代謝調節を含む広範な生理機構に関与すると考えられている。しかし、3次元的な骨芽細胞-骨細胞ネットワークから成り立つ骨組織中でのCa2+オシレーションの動態は、周囲の骨基質が障壁となるためこれまで不明であった。近年我々は、骨組織をまるごと生きた状態のままCa2+イメージングする事に成功し、骨芽細胞と骨細胞の自律性細胞内Ca2+オシレーションの存在と、それらの現象に対するメカニカルストレスの影響およびギャップ結合の関与について報告した。本論文では、蛍光ライブイメージングを用いた骨組織中のCa2+オシレーションについて最新の研究成果を交えて解説する。(著者抄録)

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  • Oscillatory intracellular Ca2+ responses in living bone

    Yoshihito Ishihara, Yasuyo Sugawara, Hiroshi Kamioka, Noriaki Kawanabe, Keiji Naruse, Takashi Yamashiro

    Journal of Oral Biosciences   56 ( 2 )   49 - 53   2014

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    Intracellular calcium (Ca2+) is an important secondary messenger that modulates many cellular processes. Its oscillatory signaling is considered to participate in the regulation of many different cell functions, including bone metabolism. However, it is not entirely clear whether Ca2+ oscillations occur between osteoblasts and osteocytes in integrated bone tissues because of the complex mineralized matrices surrounding bone cells. To address this issue, we have recently developed a novel ex vivo realtime imaging system, which made it possible to observe repetitive and autonomous oscillations in the intracellular Ca2+ concentration ([Ca2+]i) in intact chick calvarial explants. This system revealed that Ca2+ release from intracellular stores plays a key role in Ca2+ oscillations in bone cells. Additionally, gap junctions are important for the maintenance of these oscillations in osteocytes but not in osteoblasts. In this review, we describe the dynamic oscillatory elevations of Ca2+ levels that occur in osteoblasts and osteocytes in living bone. © 2014 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.

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  • Gap-junction-mediated Communication in Human Periodontal Ligament Cells Reviewed

    R. Kato, Y. Ishihara, N. Kawanabe, K. Sumiyoshi, Y. Yoshikawa, M. Nakamura, Y. Imai, T. Yanagita, H. Fukushima, H. Kamioka, T. Takano-Yamamoto, T. Yamashiro

    JOURNAL OF DENTAL RESEARCH   92 ( 7 )   635 - 640   2013.7

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    Periodontal tissue homeostasis depends on a complex cellular network that conveys cell-cell communication. Gap junctions (GJs), one of the intercellular communication systems, are found between adjacent human periodontal ligament (hPDL) cells; however, the functional GJ coupling between hPDL cells has not yet been elucidated. In this study, we investigated functional gap-junction-mediated intercellular communication in isolated primary hPDL cells. SEM images indicated that the cells were in contact with each other via dendritic processes, and also showed high anti-connexin43 (Cx43) immunoreactivity on these processes. Gap-junctional intercellular communication (GJIC) among hPDL cells was assessed by fluorescence recovery after a photobleaching (FRAP) analysis, which exhibited dye coupling between hPDL cells, and was remarkably down-regulated when the cells were treated with a GJ blocker. Additionally, we examined GJs under hypoxic stress. The fluorescence recovery and expression levels of Cx43 decreased time-dependently under the hypoxic condition. Exposure to GJ inhibitor or hypoxia increased RANKL expression, and decreased OPG expression. This study shows that GJIC is responsible for hPDL cells and that its activity is reduced under hypoxia. This is consistent with the possible role of hPDL cells in regulating the biochemical reactions in response to changes in the hypoxic environment.

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  • Assessing anteroposterior basal bone discrepancy with the Dental Aesthetic Index Reviewed

    Boyen Huang, Katsu Takahashi, Toru Yamazaki, Kazuyuki Saito, Masashi Yamori, Keita Asai, Yusuke Yoshikawa, Hiroshi Kamioka, Takashi Yamashiro, Kazuhisa Bessho

    ANGLE ORTHODONTIST   83 ( 3 )   527 - 532   2013.5

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    Objective: To investigate dental appearance and cephalometric features, using a sample of orthognathic and/or orthodontic patients. A special interest was to identify the relationship of the Dental Aesthetic Index (DAI) with anteroposterior basal bone discrepancy (APBBD) and cephalometric indicators.
    Materials and Methods: A full sample of 159 patients in two Japanese hospitals was used. Each patient was assessed with a preorthodontic dental cast and cephalometric radiography.
    Results: Malocclusion with APBBD was more prevalent among high DAI subjects (P = .034, OR = 1.04, 95% CI: 1.00-1.08), Class III malocclusion patients (P = .048, OR = 2.32, 95% CI: 1.01-5.34) and male patients (P = .008, OR = 2.96, 95% CI: 1.33-6.61). Participants scoring 88 points (the highest score in this sample) of the DAI had 16.84 times the risk of APBBD of those who scored 17 points (the lowest score in this sample). Patients with APBBD presented with a greater adjusted ANB angle (t = -8.10, P &lt; .001) and a larger adjusted A-B/NF appraisal (t = -9.65, P &lt; .001). The SNA angle (P &lt; .001), the SNB angle (P = .002), the adjusted ANB angle (P = .001), and the adjusted A-B/NF appraisal (P = .035) were associated with DAI scores in cubic regression models.
    Conclusion: This study has demonstrated a relationship between the DAI and APBBD. Feasibility of using the adjusted ANB angle and the adjusted A-B/NF appraisal to assess severity of APBBD has been confirmed. The DAI may provide a supportive method to evaluate orthognathic needs. Future investigations are indicated.

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  • Severe open bite due to traumatic condylar fractures treated nonsurgically with implanted miniscrew anchorage Reviewed

    Takeshi Yanagita, Rie Adachi, Hiroshi Kamioka, Takashi Yamashiro

    AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS   143 ( 4 )   S137 - S147   2013.4

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    This case report illustrates the use of miniscrews to treat a patient with an open bite caused by mandibular condylar fractures. The patient was 36 years old when she visited our hospital with a chief complaint of difficulty with chewing. She had suffered condylar and maxillary bone fractures in a traffic accident 6 months before her visit. She had an anterior open bite and Angle Class II molar relationships. Her mandibular midline was deviated to the right relative to the maxilla. The cephalometric analysis showed a skeletal Class II relationship. Titanium miniscrews were implanted in the bilateral maxillary buccal areas. The maxillary dentition was retracted and intruded by using elastomeric chains and miniscrews. After this treatment, an Angle Class I molar relationship was achieved, her overjet and overbite became ideal, and a good facial appearance was obtained. The total active orthodontic treatment period was 33 months. Treating an open bite with molar intrusion often leads to counter-clockwise rotation of the mandible; however, in this patient, the mandible was moved anteriorly and upward. We believe that this movement was caused by the patient's condylar fractures and the subsequent remodeling. Although there was some relapse, our results suggest that implant anchorage is useful for correcting anterior open bites originating from condylar fractures. (Am J Orthod Dentofacial Orthop 2013;143:S137-47)

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  • Ex vivo real-time observation of Ca(2+) signaling in living bone in response to shear stress applied on the bone surface. Reviewed International journal

    Yoshihito Ishihara, Yasuyo Sugawara, Hiroshi Kamioka, Noriaki Kawanabe, Satoru Hayano, Tarek A Balam, Keiji Naruse, Takashi Yamashiro

    Bone   53 ( 1 )   204 - 15   2013.3

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    Bone cells respond to mechanical stimuli by producing a variety of biological signals, and one of the earliest events is intracellular calcium ([Ca(2+)](i)) mobilization. Our recently developed ex vivo live [Ca(2+)](i) imaging system revealed that bone cells in intact bone explants showed autonomous [Ca(2+)](i) oscillations, and osteocytes specifically modulated these oscillations through gap junctions. However, the behavior and connectivity of the [Ca(2+)](i) signaling networks in mechanotransduction have not been investigated in intact bone. We herein introduce a novel fluid-flow platform for probing cellular signaling networks in live intact bone, which allows the application of capillary-driven flow just on the bone explant surface while performing real-time fluorogenic monitoring of the [Ca(2+)](i) changes. In response to the flow, the percentage of responsive cells was increased in both osteoblasts and osteocytes, together with upregulation of c-fos expression in the explants. However, enhancement of the peak relative fluorescence intensity was not evident. Treatment with 18 α-GA, a reversible inhibitor of gap junction, significantly blocked the [Ca(2+)](i) responsiveness in osteocytes without exerting any major effect in osteoblasts. On the contrary, such treatment significantly decreased the flow-activated oscillatory response frequency in both osteoblasts and osteocytes. The stretch-activated membrane channel, when blocked by Gd(3+), is less affected in the flow-induced [Ca(2+)](i) response. These findings indicated that flow-induced mechanical stimuli accompanied the activation of the autonomous [Ca(2+)](i) oscillations in both osteoblasts and osteocytes via gap junction-mediated cell-cell communication and hemichannel. Although how the bone sense the mechanical stimuli in vivo still needs to be elucidated, the present study suggests that cell-cell signaling via augmented gap junction and hemichannel-mediated [Ca(2+)](i) mobilization could be involved as an early signaling event in mechanotransduction.

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  • The early mouse 3D osteocyte network in the presence and absence of mechanical loading. Reviewed International journal

    Yasuyo Sugawara, Hiroshi Kamioka, Yoshihito Ishihara, Naoko Fujisawa, Noriaki Kawanabe, Takashi Yamashiro

    Bone   52 ( 1 )   189 - 96   2013.1

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    Osteocytes are considered to act as mechanosensory cells in bone. They form a functional synctia in which their processes become interconnected to constitute a three-dimensional (3D) network. Previous studies reported that in mice, the two-dimensional osteocyte network becomes progressively more regular as they grow, although the key factors governing the arrangement of the osteocyte network during bone growth remain unknown. In this study, we characterized the 3D formation of the osteocyte network during bone growth. Morphological skeletal changes have been reported to occur in response to mechanical loading and unloading. In order to evaluate the effect of mechanical unloading on osteocyte network formation, we subjected newborn mice to sciatic neurectomy in order to immobilize their left hind limb as an unloading model. The osteocyte network was visualized by staining osteocyte cell bodies and processes with fluorescently labeled phalloidin. First, we compared the osteocyte network in the femora of embryonic and 6-week-old mice in order to understand the morphological changes that occur with normal growth and mechanical loading. In embryonic mice, the osteocyte network in the femur cortical bone displayed a random cell body distribution, non-directional orientation of cell processes, and irregularly shaped cells. In 6-week-old mice, the 3D network contained spindle-shaped osteocytes, which were arranged parallel to the longitudinal axis of the femur. In addition, more and longer cell processes radiated from each osteocyte. Second, we compared the cortical osteocyte networks of 6-week-old mice that had or had not undergone sciatic neurectomy in order to evaluate the effect of unloading on osteocyte network formation. The osteocyte network formation in both cortical bone and cancellous bone was affected by mechanical loading. However, there were differences in the extent of network formation between cortical bone and cancellous bone in response to mechanical loading with regard to the orientation, nuclear shape and branch formation.

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  • 岡山大学大学院歯科矯正学分野研究室における2008年から2012年の5年間の岡山大学の学生および職員に対する矯正相談の実態調査 Reviewed

    菅原康代, 村上隆, 田川淳平, 星島光博, 柳田剛志, 石原嘉人, 片岡伴記, 伊藤慎将, 川邉紀章, 上岡寛, 山城隆

    岡山歯学会雑誌   32 ( 2 )   43 - 47   2013

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    2008〜2012年に岡山大学大学院歯科矯正学分野医局を訪れた岡山大学学生128名と職員11名の計139名(男性40名、女性99名)を対象に、矯正相談の実態について調査した。その結果、相談の契機は掲示されたポスターによる者が最も多く75名54%であった。学年別では1年生48名、2年生21名、3年生15名、4年生18名で、学部別では医学部保健学科と歯学部が最も多く各18名であった。主訴の最多は上顎前突34名(24.5%)で、次いで上顎の叢生27名(19.4%)であった。相談後に矯正を開始したのは学年別で最多は1年生で相談者のうち35名72.9%、全治療開始者の40.7%を占めた。以上より、矯正相談は、歯並びのコンプレックス等の解決や福利厚生として有効であることが示唆された。

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  • Relationship between orthodontic expertise and perception of need for orthodontic treatment for mandibular protrusion in Japan Reviewed

    Takashi Murakami, Akihito Fujii, Yuya Kawabata, Hiroaki Takakura, Rie Yamaue, Tarek Abdulsamad Ali Balam, Shingo Kuroda, Noriaki Kawanabe, Hiroshi Kamioka, Takashi Yamashiro

    Acta Medica Okayama   67 ( 5 )   277 - 283   2013

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    The aims of this study were to investigate how the Peer Assessment Rating (PAR index) predicts the perceived need for orthodontic treatment of mandibular protrusion in Japanese subjects, and to elucidate whether the perceived need for treatment was affected by the raters' orthodontic expertise. The subjects were 110 dental students and 32 orthodontists. We showed them casts of 10 untreated mandibular protrusion cases and gave them a questionnaire in which they had to describe their perceptions of the orthodontic treatment needs using a 10-point visual analog scale (VAS). The PAR index was used for cast evaluation. The PAR index scores showed significant correlations with the VAS scores. In casts with a low PAR score, there were no differences in the VAS scores between orthodontists and students. In casts with a PAR score greater than 23, the orthodontists perceived a significantly greater treatment need than did the students
    for scores of 22, 28, and 29, students who had received orthodontic treatment themselves were more likely to perceive the treatment need. The PAR index is a good clinical predictor for assessing the perceived treatment needs for mandibular protrusion. Perception of the need for orthodontic treatment for mandibular protrusion depended on the degree of orthodontic expertise in Japanese subjects. © 2013 by Okayama University Medical School.

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  • Promotion of Ccn2 expression and osteoblastic differentiation by actin polymerization, which is induced by laminar fluid flow stress

    Tadashi Honjo, Satoshi Kubota, Hiroshi Kamioka, Yasuyo Sugawara, Yoshihito Ishihara, Takashi Yamashiro, Masaharu Takigawa, Teruko Takano-Yamamoto

    JOURNAL OF CELL COMMUNICATION AND SIGNALING   6 ( 4 )   225 - 232   2012.12

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    Fluid flow stress (FSS) is a major mechanical stress that induces bone remodeling upon orthodontic tooth movement, whereas CCN family protein 2 (CCN2) is a potent regenerator of bone defects. In this study, we initially evaluated the effect of laminar FSS on Ccn2 expression and investigated its mechanism in osteoblastic MC3T3-E1 cells. The Ccn2 expression was drastically induced by uniform FSS in an intensity dependent manner. Of note, the observed effect was inhibited by a Rho kinase inhibitor Y27632. Moreover, the inhibition of actin polymerization blocked the FSS-induced activation of Ccn2, whereas inducing F-actin formation using cytochalasin D and jasplakinolide enhanced Ccn2 expression in the same cells. Finally, F-actin formation was found to induce osteoblastic differentiation. In addition, activation of cyclic AMP-dependent kinase, which inhibits Rho signaling, abolished the effect of FSS. Collectively, these findings indicate the critical role of actin polymerization and Rho signaling in CCN2 induction and bone remodeling provoked by FSS.

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  • Microscale fluid flow analysis in a human osteocyte canaliculus using a realistic high-resolution image-based three-dimensional model. Reviewed International journal

    Hiroshi Kamioka, Yoshitaka Kameo, Yuichi Imai, Astrid D Bakker, Rommel G Bacabac, Naoko Yamada, Akio Takaoka, Takashi Yamashiro, Taiji Adachi, Jenneke Klein-Nulend

    Integrative biology : quantitative biosciences from nano to macro   4 ( 10 )   1198 - 206   2012.10

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    Osteocytes play a pivotal role in the regulation of skeletal mass. Osteocyte processes are thought to sense the flow of interstitial fluid that is driven through the osteocyte canaliculi by mechanical stimuli placed upon bone, but how this flow elicits a cellular response is virtually unknown. Modern theoretical models assume that osteocyte canaliculi contain ultrastructural features that amplify the fluid flow-derived mechanical signal. Unfortunately the calcified bone matrix has considerably hampered studies on the osteocyte process within its canaliculus. Using one of the few ultra high voltage electron microscopes (UHVEM) available worldwide, we applied UHVEM tomography at 2 MeV to reconstruct unique three-dimensional images of osteocyte canaliculi in 1 μm sections of human bone. A realistic three-dimensional image-based model of a single canaliculus was constructed, and the fluid dynamics of a Newtonian fluid flow within the canaliculus was analyzed. We created virtual 2.2 nm thick sections through a canaliculus and found that traditional TEM techniques create a false impression that osteocyte processes are directly attached to the canalicular wall. The canalicular wall had a highly irregular surface and contained protruding axisymmetric structures similar in size and shape to collagen fibrils. We also found that the microscopic surface roughness of the canalicular wall strongly influenced the fluid flow profiles, whereby highly inhomogeneous flow patterns emerged. These inhomogeneous flow patterns may induce deformation of cytoskeletal elements in the osteocyte process, thereby amplifying mechanical signals. Based on these observations, new and realistic models can be developed that will significantly enhance our understanding of the process of mechanotransduction in bone.

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  • Increasing participation of sclerostin in postnatal bone development, revealed by three-dimensional immunofluorescence morphometry. Reviewed International journal

    Takashi Watanabe, Yoshihiro Tamamura, Akiyoshi Hoshino, Yuji Makino, Hiroshi Kamioka, Teruo Amagasa, Akira Yamaguchi, Tadahiro Iimura

    Bone   51 ( 3 )   447 - 58   2012.9

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    Confocal immunofluorescence tiling imaging revealed the spatio-temporal distributions of osterix and sclerostin in femurs from 3-day-old, 2-week-old and 4-week-old rats to be reciprocally exclusive at the tissue level. Further quantitative three-dimensional immuno fluorescence morphometry demonstrated the increasing distribution of sclerostin in the osteocytic lacuno-canalicular system specifically in diaphysis, which paralleled the cooperative participation and depletion of osterix and β-catenin in adjacent periosteum cells. Treating MC3T3-E1 cells with BIO (a GSK3 inhibitor) induced the stabilization of β-catenin and nuclear translocation of osterix, and negatively regulated osteocalcin/BGLAP and Dmp1. These results collectively demonstrate that the increasing distribution of sclerostin in diaphyseal cortical bone appears to be involved in the attenuation of osterix and β-catenin in adjacent periosteum cells, thus possibly contributing to osteoblast maturation and reducing the osteoblast formation at this bone site. Our confocal microscopy-based imaging analyses provide a comprehensive and detailed view of the spatio-temporal distribution of sclerostin, β-catenin and osterix at the tissue to subcellular level in a coherent manner, and uncovered their spatio-temporal cooperation in postnatal bone development, thus providing evidence that they link skeletogenic growth and functional bone development.

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  • In situ imaging of the autonomous intracellular Ca(2+) oscillations of osteoblasts and osteocytes in bone. Reviewed International journal

    Yoshihito Ishihara, Yasuyo Sugawara, Hiroshi Kamioka, Noriaki Kawanabe, Hiroshi Kurosaka, Keiji Naruse, Takashi Yamashiro

    Bone   50 ( 4 )   842 - 52   2012.4

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    Bone cells form a complex three-dimensional network consisting of osteoblasts and osteocytes embedded in a mineralized extracellular matrix. Ca(2+) acts as a ubiquitous secondary messenger in various physiological cellular processes and transduces numerous signals to the cell interior and between cells. However, the intracellular Ca(2+) dynamics of bone cells have not been evaluated in living bone. In the present study, we developed a novel ex-vivo live Ca(2+) imaging system that allows the dynamic intracellular Ca(2+) concentration ([Ca(2+)](i)) responses of intact chick calvaria explants to be observed without damaging the bone network. Our live imaging analysis revealed for the first time that both osteoblasts and osteocytes display repetitive and autonomic [Ca(2+)](i) oscillations ex vivo. Thapsigargin, an inhibitor of the endoplasmic reticulum that induces the emptying of intracellular Ca(2+) stores, abolished these [Ca(2+)](i) responses in both osteoblasts and osteocytes, indicating that Ca(2+) release from intracellular stores plays a key role in the [Ca(2+)](i) oscillations of these bone cells in intact bone explants. Another possible [Ca(2+)](i) transient system to be considered is gap junctional communication through which Ca(2+) and other messenger molecules move, at least in part, across cell-cell junctions; therefore, we also investigated the role of gap junctions in the maintenance of the autonomic [Ca(2+)](i) oscillations observed in the intact bone. Treatment with three distinct gap junction inhibitors, 18α-glycyrrhetinic acid, oleamide, and octanol, significantly reduced the proportion of responsive osteocytes, indicating that gap junctions are important for the maintenance of [Ca(2+)](i) oscillations in osteocytes, but less in osteoblasts. Taken together, we found that the bone cells in intact bone explants showed autonomous [Ca(2+)](i) oscillations that required the release of intracellular Ca(2+) stores. In addition, osteocytes specifically modulated these oscillations via cell-cell communication through gap junctions, which maintains the observed [Ca(2+)](i) oscillations of bone cells.

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  • Roles of Heparan Sulfate Sulfation in Dentinogenesis Reviewed

    Satoru Hayano, Hiroshi Kurosaka, Takeshi Yanagita, Ina Kalus, Fabian Milz, Yoshihito Ishihara, Md. Nurul Islam, Noriaki Kawanabe, Masahiro Saito, Hiroshi Kamioka, Taiji Adachi, Thomas Dierks, Takashi Yamashiro

    JOURNAL OF BIOLOGICAL CHEMISTRY   287 ( 15 )   12217 - 12229   2012.4

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    Cell surface heparan sulfate (HS) is an essential regulator of cell signaling and development. HS traps signaling molecules, like Wnt in the glycosaminoglycan side chains of HS proteoglycans (HSPGs), and regulates their functions. Endosulfatases Sulf1 and Sulf2 are secreted at the cell surface to selectively remove 6-O-sulfate groups from HSPGs, thereby modifying the affinity of cell surface HSPGs for its ligands. This study provides molecular evidence for the functional roles of HSPG sulfation and desulfation in dentinogenesis. We show that odontogenic cells are highly sulfated on the cell surface and become desulfated during their differentiation to odontoblasts, which produce tooth dentin. Sulf1/Sulf2 double null mutant mice exhibit a thin dentin matrix and short roots combined with reduced expression of dentin sialophosphoprotein (Dspp) mRNA, encoding a dentin-specific extracellular matrix precursor protein, whereas single Sulf mutants do not show such defective phenotypes. In odontoblast cell lines, Dspp mRNA expression is potentiated by the activation of the Wnt canonical signaling pathway. In addition, pharmacological interference with HS sulfation promotes Dspp mRNA expression through activation of Wnt signaling. On the contrary, the silencing of Sulf suppresses the Wnt signaling pathway and subsequently Dspp mRNA expression. We also show that Wnt10a protein binds to cell surface HSPGs in odontoblasts, and interference with HS sulfation decreases the binding affinity of Wnt10a for HSPGs, which facilitates the binding of Wnt10a to its receptor and potentiates the Wnt signaling pathway, thereby up-regulating Dspp mRNA expression. These results demonstrate that Sulf-mediated desulfation of cellular HSPGs is an important modification that is critical for the activation of the Wnt signaling in odontoblasts and for production of the dentin matrix.

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  • Stage-specific embryonic antigen-4 identifies human dental pulp stem cells Reviewed

    Noriaki Kawanabe, Satoko Murata, Hiroaki Fukushima, Yoshihito Ishihara, Takeshi Yanagita, Emmy Yanagita, Mitsuaki Ono, Hiroshi Kurosaka, Hiroshi Kamioka, Tomoo Itoh, Takuo Kuboki, Takashi Yamashiro

    EXPERIMENTAL CELL RESEARCH   318 ( 5 )   453 - 463   2012.3

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    Embryonic stem cell-associated antigens are expressed in a variety of adult stem cells as well as embryonic stem cells. In the present study, we investigated whether stage-specific embryonic antigen (SSEA)-4 can be used to isolate dental pulp (DP) stem cells. DP cells showed plastic adherence, specific surface antigen expression, and multipotent differentiation potential, similar to mesenchymal stem cells (MSC). SSEA-4+ cells were found in cultured DP cells in vitro as well as in DP tissue in vivo. Flow cytometric analysis demonstrated that 45.5% of the DP cells were SSEA-4+. When the DP cells were cultured in the presence of all-trans-retinoic acid, marked downregulation of SSEA-3 and SSEA-4 and the upregulation of SSEA-1 were observed. SSEA-4+ DP cells showed a greater telomere length and a higher growth rate compared to ungated and SSEA-4 - cells. A clonal assay demonstrated that 65.5% of the SSEA-4+ DP cells had osteogenic potential, and the SSEA-4+ clonal DP cells showed multilineage differentiation potential toward osteoblasts, chondrocytes, and neurons in vitro. In addition, the SSEA-4+ DP cells had the capacity to form ectopic bone in vivo. Thus, our results suggest that SSEA-4 is a specific cell surface antigen that can be used to identify DP stem cells. (C) 2012 Elsevier Inc. All rights reserved.

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  • Intradermal injection of Botulinum toxin type A alleviates infraorbital nerve constriction-induced thermal hyperalgesia in an operant assay Reviewed

    A. Kumada, Y. Matsuka, I. Spigelman, K. Maruhama, Y. Yamamoto, J. K. Neubert, T. A. Nolan, K. Watanabe, K. Maekawa, H. Kamioka, T. Yamashiro, T. Kuboki, K. Oguma

    JOURNAL OF ORAL REHABILITATION   39 ( 1 )   63 - 72   2012.1

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    Recent studies have shown that infraorbital nerve constriction (IoNC)-induced mechanical allodynia has been attenuated by administration of highly purified 150-kDa Botulinum neurotoxin type A (BoNT/A). Here, we extend these studies to determine whether BoNT/A could attenuate IoNC-induced symptoms of thermal hyperalgesia. Instead of testing head withdrawal thresholds, a thermal operant assay was used to evaluate cortical processing of sensory input following IoNC. In this assay, a fasted rats desire to obtain a food reward (sweetened condensed milk) is coupled to its ability to tolerate facial contact with a warm (45 degrees C) thermode. Bilateral IoNC decreased the ratio of thermode contact duration/event, which is an indicative of thermal hyperalgesia. BoNT/A injection intradermally in the area of infraorbital nerve (IoN) innervation 7 days after IoNC resulted in decreased number of facial contacts and increased the ratio of contact duration/event (measured at 14 days after IoNC). The BoNT/A (2200 pg) effects were dose dependent and statistically significant at 100 and 200 pg (P &lt; 0.05). Complete reversal of thermal hyperalgesia symptoms was obtained with a 200-pg dose, without affecting sham rat behaviour. Off-site (neck) injection of BoNT/A did not relieve thermal hyperalgesia, while co-injection of BoNT/A with a neutralising antibody in the area of IoN innervation prevented relief of thermal hyperalgesia. Neither IoNC nor BoNT/A injection affected operant assay parameters with a 24 degrees C thermode, indicating selectivity of thermal hyperalgesia measurements. These results strongly suggest that intradermal injection of BoNT/A in the area of IoN innervation alleviates IoNC-induced thermal hyperalgesia in an operant assay.

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  • Induction of CCN2/CTGF by laminar fluid flow stress, which is mediated by the actin cytoskeleton in osteoblastic cells Reviewed

    Honjo, T, S. Kubota, H. Kamioka, Y. Sugawara, Y. Ishihara, T. Yamashiro, M. Takigawa, T. Takano-Yamamoto

    J Cell Commun Signal.   6   225 - 232   2012

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  • ミニスクリューを用いて重度の前歯部開咬を改善した症例 Reviewed

    冨田奈緒, 上岡寛, 植田紘貴, 加野小奈美, 山城隆

    中・四矯正歯誌   24 ( 1 )   63 - 71   2012

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  • ミニスクリューを使用して治療を行ったhigh mandibular plane angleを呈する下顎後退症例

    村上 隆, 上岡 寛, 山城 隆

    中・四国矯正歯科学会雑誌   23 ( 1 )   53 - 59   2011.8

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  • The three-dimensional morphometry and cell-cell communication of the osteocyte network in chick and mouse embryonic calvaria. Reviewed International journal

    Yasuyo Sugawara, Ryoko Ando, Hiroshi Kamioka, Yoshihito Ishihara, Tadashi Honjo, Noriaki Kawanabe, Hiroshi Kurosaka, Teruko Takano-Yamamoto, Takashi Yamashiro

    Calcified tissue international   88 ( 5 )   416 - 24   2011.5

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    The study of osteocytes has progressed in chicks. We examined whether chick osteocyte data can be applied to other species. We used mice for comparison because they are common clinical tools in biomedical research and useful for future study. We analyzed the three-dimensional (3D) osteocyte network and gap junctional intercellular communication (GJIC) in living embryonic calvaria for the anatomical features. Embryonic parietal bones were stained with fluorescently labeled phalloidin and observed using confocal laser scanning microscopy. GJIC between osteocytes in chick and mouse parietal bone was assessed using fluorescence recovery after photobleaching (FRAP). The values for one chick and mouse osteocyte, respectively, were calculated as follows: cell processes 1,131 ± 139 μm, 2,668 ± 596 μm; surface area 1,128 ± 358 μm(2), 2,654 ± 659 μm(2); and cell volume 455 ± 90 μm(3), 1,328 ± 210 μm(3). The density of 3D osteocyte processes in the bone matrix was not significantly different. FRAP analysis showed dye coupling among osteocytes in chick and mouse bone. The fluorescence intensity recovered to 49.0 ± 2.4% in chicks and 39.9 ± 2.4% in mice after 5 minutes. Fluorescence recovery was similar within 4 minutes. The difference in osteocyte size between the two species might have affected their functions. Osteocyte processes in the two species may sense similarly changes in the exterior environment. We successfully conducted morphological and functional analyses of the osteocyte network in chicks and mice. The size of the osteocytes in bone differed between the two species.

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  • Sox9 Expression during Fracture Repair Reviewed

    Yuko Shintaku, Takashi Murakami, Takeshi Yanagita, Noriaki Kawanabe, Tomohiro Fukunaga, Kiyomi Matsuzaki, Setsuko Uematsu, Yasuhiro Yoshida, Hiroshi Kamioka, Teruko Takano-Yamamoto, Kenji Takada, Takashi Yamashiro

    CELLS TISSUES ORGANS   194 ( 1 )   38 - 48   2011

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    The molecular and cellular mechanisms involved in bone development provide an insight into the nature of bone regeneration. Sox9 is a key transcription factor for chondrogenesis and is also expressed in osteochondroprogenitors during embryonic bone development. However, it has not been determined whether Sox9-expressing cells appear during fracture repair other than in the cartilaginous callus. On the other hand, the difference between bone development and repair is that the motion of the fractured segments is associated with the subsequent fate decision of osteochondrogenic precursors between osteogenesis or chondrogenesis, but the underlying mechanism of this still has to be elucidated. We herein evaluate whether Sox9 expressing cells appear during osseous regeneration in the initial stages of fracture healing in vivo. We also investigated the association between Sox9 induction and mechanical stress and the role of Runx1 expression. As a result, Sox9- and Runx1-expressing cells were detected in the periosteal callus together with Runx2 expression. Their expression levels were significantly downregulated during its ossification, as observed in embryonic bone development. The application of cyclic tension to isolated and cultured stromal cells resulted in the upregulation and maintenance of Sox9 mRNA expression in vitro. These results showed that as in early skeletal development, Sox9- and Runx1-expressing precursor cells first appear in the periosteal callus as an early fracture repair response. Our findings also suggested that the mechanical environment modulates Sox9 expression levels in osteochondrogenic precursors and consequently influences their fate decision between osteogenic and chondrogenic lineage commitment. Copyright (C) 2011 S. Karger AG, Basel

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  • 岡山大学病院矯正歯科を受診しII期治療を開始した初診患者の実態調査

    片岡伴記, 上岡寛, 川邊紀章, 本城正, 菅原康代, 村上隆, 柳田剛志, 石原嘉人, 山城隆

    中四国矯正歯科学会雑誌   23 ( 1 )   83 - 92   2011

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  • Application of bioimaging to osteocyte biology

    Hiroshi Kamioka, Taiji Adachi

    Clinical Reviews in Bone and Mineral Metabolism   8 ( 4 )   170 - 178   2010.12

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    In a variety of scientific fields, it is helpful to visualize natural phenomena. Newly developed methods of visualization often lead to breakthroughs in scientific fields. Especially, in the bioscience field, it is significant to reveal temporal-spatial responses in cells while visualizing molecular phenomena. Such visualization may provide information to help understand cellular behavior in response to an extracellular stimulus. Although osteocytes are the most abundant cells in bone, it has been difficult to study their biological nature because they are embedded in hard bone tissue. So, the real 3D structure of osteocytes was not clarified until recently. On the other hand, a newly developed technique of visualization was recently introduced into bone cell biology. In this review, we introduce and review our application of a bioimaging technique to reveal the morphology of osteocytes as well as part of their biological nature. © Springer Science+Business Media, LLC 2010.

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  • Isolation of multipotent stem cells in human periodontal ligament using stage-specific embryonic antigen-4

    Noriaki Kawanabe, Satoko Murata, Kaoru Murakami, Yoshihito Ishihara, Satoru Hayano, Hiroshi Kurosaka, Hiroshi Kamioka, Teruko Takano-Yamamoto, Takashi Yamashiro

    DIFFERENTIATION   79 ( 2 )   74 - 83   2010.2

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    The periodontal ligament (PDL) comprises adult stem cells, which are responsible for periodontal tissue regeneration. In the present study, we investigated the specific profile of the stem cells in the human PDL. Microscopic analysis demonstrated that PDL cells showed a fibroblastic appearance, forming flat and loose aggregates. PDL cells expressed embryonic stem cell-associated antigens (SSEA-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, OCT4, NANOG, SOX2, and REX1, and alkaline phosphatase activity), as well as conventional mesenchymal stem cell markers. When PDL cells were cultured in the presence of all-trans-retinoic acid, the numbers of SSEA-3+ and SSEA-4+ PDL cells were significantly decreased, while that of SSEA-1+ was increased. SSEA-4+ PDL cells showed a greater telomere length and growth rate. SSEA-4+ PDL cells exhibited the potential to generate specialized cells derived from three embryonic germ layers: mesodermal (adipocytes, osteoblasts, and chondrocytes), ectodermal (neurons), and endodermal (hepatocytes) lineages. Our findings demonstrated that SSEA-4, a major antigen to distinguish human embryonic stem cells, could also be used to identify multipotent stem cells in the PDL. Hence, SSEA-4+ human PDL cells appear to be a promising source of stem cells for regenerative medicine. (C) 2009 International Society of Differentiation. Published by Elsevier Ltd. All rights reserved.

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  • 岡山大学病院矯正歯科における来院患者の実態調査

    白賀 のり子, 上岡 寛, 片岡 伴記, 植田 紘貴, 安藤 涼子, 石原 嘉人, 柳田 剛志, 村上 隆, 黒坂 寛, 菅原 康代, 本城 正, 川邉 紀章, 山城 隆

    岡山歯学会雑誌   28 ( 2 )   107 - 114   2009.12

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    岡山大学病院矯正歯科では2000年度から術前の顎機能検査とMRI検査を取り入れ、より精度の高い診断の基に個々の患者の様々な主訴に応じた治療を行っており、患者数も2001年度の184名から2008年度には209名に増加傾向を示した。2009年4月の1ヵ月間に来院した患者を対象に診療体制に関するアンケート調査を実施、回収された269名について分析した。その結果、対象の性別は女性が男性の1.5倍多く、年齢は成人患者が過半数を占め、矯正治療開始時の主訴は歯ならびの外観63.7%、顔や顎の形9.9%、噛み難い6.9%、発音し難い2.7%、顎関節疾患3.1%、虫歯や歯周病予防3.4%であった。来院のきっかけはかかりつけ医の紹介が35.1%と最も多く、来院動機は高度な専門医療が受けられると思うからが27.9%であった。以上より、岡山大学病院矯正歯科の特色であるインプラントアンカー治療や舌側矯正装置による治療を、地域との連携を深めながらより一層周知すること、さらなる技術の向上に努める必要性が示唆された。

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  • Camouflage treatment using implant anchorage in a skeletal Class III patient

    Hiroshi Kamioka, Noriko Shiraga, Teruko Takano-Yamamoto, Takashi Yamashiro

    Orthodontic Waves   68 ( 4 )   171 - 177   2009.12

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    This is a case report of a female patient (10 years 3 months) whose chief complaint was edge-to-edge bite. At the initial examination, the diagnosis was severe skeletal Class III. In first-phase treatment, growth modification was planned. Although forward and downward maxillary growth was observed, skeletal Class III still remained. In the second-phase treatment, implant anchorage was applied for distal movement of the mandibular teeth. A favorable occlusal relationship was achieved, and acceptable stomatognathic function was maintained after 2 years of retention. © 2009 Elsevier Ltd and the Japanese Orthodontic Society.

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  • Asymmetric intercellular communication between bone cells: propagation of the calcium signaling. Reviewed International journal

    Taiji Adachi, Yuki Aonuma, Keisuke Taira, Masaki Hojo, Hiroshi Kamioka

    Biochemical and biophysical research communications   389 ( 3 )   495 - 500   2009.11

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    Bone functional adaptation by remodeling is achieved by harmonized activities of bone cells in which osteocytes in the bone matrix are believed to play critical roles in sensing mechanical stimuli and transmitting signals to osteoclasts/osteoblasts on the bone surface in order to regulate their bone remodeling activities through the lacuno-canalicular network with many slender osteocytic processes. In this study, we investigated the intercellular communication between bone cells, particularly focusing on its directionality, through in vitro observations of the calcium signaling response to mechanical stimulus and its propagation to neighboring cells (NCs). Direct mechanical stimulus was applied to isolated bone cells from chick calvariae, osteocytes (Ocys) and bone surface cells (BSCs) mainly containing osteoblasts, and the percentage of calcium signaling propagation from the stimulated cell to NCs was analyzed. The results revealed that, regardless of the type of stimulated cell, the signaling propagated to BSCs with a significantly higher percentage, implying that calcium signaling propagation between bone cells strongly depends on the type of receiver cell and not the transmitter cell. In addition, in terms of mutual communication between Ocys and BSCs, the percentage of propagation from Ocys to BSCs is significantly higher than that in the opposite direction, suggesting that the calcium signaling mainly propagates asymmetrically with a bias from Ocys in bone matrix to BSCs on bone surfaces. This asymmetric communication between Ocys and BSCs suggests that osteocytic mechanosensing and cellular communications, which significantly affect bone surface remodeling activities to achieve functional adaptation, seem to be well coordinated and active at the location of biologically suitable and mechanically sensitive regions close to the bone surfaces.

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  • Osteocyte calcium signaling response to bone matrix deformation. Reviewed International journal

    Taiji Adachi, Yuki Aonuma, Shin-ichi Ito, Mototsugu Tanaka, Masaki Hojo, Teruko Takano-Yamamoto, Hiroshi Kamioka

    Journal of biomechanics   42 ( 15 )   2507 - 12   2009.11

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    Osteocytes embedded in calcified bone matrix have been widely believed to play important roles in mechanosensing to achieve adaptive bone remodeling in a changing mechanical environment. In vitro studies have clarified several types of mechanical stimuli such as hydrostatic pressure, fluid shear stress, and direct deformation influence osteocyte functions. However, osteocyte response to mechanical stimuli in the bone matrix has not been clearly understood. In this study, we observed the osteocyte calcium signaling response to the quantitatively applied deformation in the bone matrix. A novel experimental system was developed to apply deformation to cultured bone tissue with osteocytes on a microscope stage. As a mechanical stimulus to the osteocytes in bone matrix, in-plane shear deformation was applied using a pair of glass microneedles to bone fragments, obtained from 13-day-old embryonic chick calvariae. Deformation of bone matrix and cells was quantitatively evaluated using an image correlation method by applying for differential interference contrast images of the matrix and fluorescent images of immunolabeled osteocytes, together with imaging of the cellular calcium transient using a ratiometric method. As a result, it was confirmed that the newly developed system enables us to apply deformation to bone matrix and osteocytes successfully under the microscope without significant focal plane shift or deviation from the observation view field. The system could be a basis for further development to investigate the mechanosensing mechanism of osteocytes in bone matrix through examination of various types of rapid biochemical signaling responses and intercellular communication induced by matrix deformation.

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  • A method for observing silver-stained osteocytes in situ in 3-microm sections using ultra-high voltage electron microscopy tomography. Reviewed International journal

    Hiroshi Kamioka, Sakhr A Murshid, Yoshihito Ishihara, Naoko Kajimura, Toshiaki Hasegawa, Ryoko Ando, Yasuyo Sugawara, Takashi Yamashiro, Akio Takaoka, Teruko Takano-Yamamoto

    Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada   15 ( 5 )   377 - 83   2009.10

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    Osteocytes are surrounded by hard bone matrix, and it has not been possible previously to directly observe the in situ architecture of osteocyte morphology in bone. Electron microscope tomography, however, is a technique that has the unique potential to provide three-dimensional (3D) visualization of cellular ultrastructure. This approach is based on reconstruction of 3D volumes from a tilt series of electron micrographs of cells, and resolution at the nanometer level has been achieved. We applied electron microscope tomography to thick sections of silver-stained osteocytes in bone using a Hitachi H-3000 ultra-high voltage electron microscope equipped with a 360 degrees tilt specimen holder, at an accelerating voltage of 2 MeV. Osteocytes with numerous processes and branches were clearly seen in the serial tilt series acquired from 3-microm-thick sections. Reconstruction of young osteocytes showed the 3D topographic morphology of the cell body and processes at high resolution. This morphological data on osteocytes should provide useful information to those who study osteocyte physiology and the several models used to explain their mechanosensory properties.

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  • Corrigendum to "botulinum toxin type A (150 kDa) decreases exaggerated neurotransmitter release from trigeminal ganglion neurons and relieves neuropathy behaviors induced by infraorbital nerve constriction" [Neuroscience 159 (2009) 1422-1429] (DOI:10.1016/j.neuroscience.2009.01.066) Reviewed

    Y. Kitamura, Y. Matsuka, I. Spigelman, Y. Ishihara, Y. Yamamoto, W. Sonoyama, H. Kamioka, T. Yamashiro, T. Kuboki, K. Oguma

    Neuroscience   161 ( 3 )   950   2009.7

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  • Bimaxillary protrusion with masseter muscle hypertrophy treated with titanium screw anchorage and masseter surgical reduction

    Takashi Hashimoto, Shingo Kuroda, Hiroshi Kamioka, Katsuaki Mishima, Toshio Sugahara, Teruko Takano-Yamamoto

    AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS   135 ( 4 )   536 - 548   2009.4

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    This case report describes the treatment of a patient with bimaxillary protrusion and masseter muscle hypertrophy. At age 21 years 7 months, this woman had temporomandibular disorder (TMD) symptoms, severe bimaxillary protrusion, and a prominent mandibular angle with facial asymmetry. After an attempt to alleviate the TMD symptoms with occlusal splint stabilization, portions of the masseter muscle and the mandible were surgically removed. Titanium screws were placed bilaterally in both arches, and a retraction force was applied. After active treatment for 38 months, the convexity of the facial profile with lip protrusion was improved remarkably, and good occlusion was achieved. The prominent mandibular angle with facial asymmetry was improved as a result of the surgical reduction of the masseter muscle and the modeling ostectomy near the masseteric tuberosity. The TMD symptoms disappeared, and the jaw movement pattern became normal. Therefore, our results suggest that this combination treatment would be useful for masseter muscle hypertrophy for morphologic and functional problems. (Am J Orthod Dentofacial Orthop 2009; 135:536-48)

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  • 上下顎移動術を適応した顎偏位を伴う骨格性下顎前突症例

    本城正, 上岡 寛, 黒坂 寛, 山本照子, 山城 隆

    中・四国矯正歯科学会雑誌   21 ( 1 )   41 - 53   2009

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  • [Osteocytes and mechanical stress]. Reviewed

    Hiroshi Kamioka, Takashi Yamashiro

    Clinical calcium   18 ( 9 )   1287 - 93   2008.9

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    A number of fascinating studies suggest that osteocytes play an important role in bone metabolism, especially in its regulation by mechanical stimuli. However, it has been still discussed how the osteocytes were activated by mechanical stimuli. For example, direct mechanical load applied to cells at levels measured to occur in humans in vivo do not affect bone cells in vitro . In order to solve the contradiction, theories based on fluid flow-induced shear stress stimulation around osteocytes in bone have appeared with the most prominence. In addition, I would like to introduce recent hypothesis to mechanically stimulate osteocyte, such as strain amplification by radial hoop strains, flow induced integrin mediated signaling, and osteocyte lacuna strain. To validate the availability of these hypothesis, it is important to observe 3-dimensional osteocyte morphology as well as its interaction with pericellular matrix.

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  • 【メカニカルストレスと骨】骨細胞とメカニカルストレス

    上岡 寛, 山城 隆

    Clinical Calcium   18 ( 9 )   1287 - 1293   2008.8

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    骨は機械的刺激を受けマクロな変化を遂げる。その結果は、骨に存在する細胞群が引き起こすミクロな変化の集積である。このような変化を生じるためには、骨の細胞は何らかの方法で機械的刺激を感知しなければならない。実際に、骨の形態を変化させるのは骨芽細胞および破骨細胞であるが、機械的刺激を感知し、これらの細胞の役割を調節しているのが骨細胞であると考えられている。骨細胞は骨基質に囲まれている特異な環境のために、実際にどのようにして機械的刺激を感知しているのかは明らかではない。しかしながら、近年、生体工学的な試みにより、さまざまな骨細胞の機械的刺激の受容機構が提案されてきた。そこで、本稿ではこれらの説を紹介するとともに、骨細胞およびその周囲骨基質の三次元構造を求めることの重要性について解説していきたい。(著者抄録)

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  • The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes. Reviewed International journal

    Yasuyo Sugawara, Ryoko Ando, Hiroshi Kamioka, Yoshihito Ishihara, Sakhr A Murshid, Ken Hashimoto, Noriyuki Kataoka, Katsuhiko Tsujioka, Fumihiko Kajiya, Takashi Yamashiro, Teruko Takano-Yamamoto

    Bone   43 ( 1 )   19 - 24   2008.7

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    Osteocytes acquire their stellate shape during the process of changing from osteoblasts in bone. Throughout this process, dynamic cytoskeletal changes occur. In general, changes of the cytoskeleton affect cellular mechanical properties. Mechanical properties of living cells are connected with their biological functions and physiological processes. In this study, we for the first time analyzed elastic modulus, a mechanical property of bone cells. Bone cells in embryonic chick calvariae and in isolated culture were identified using fluorescently labeled phalloidin and OB7.3, a chick osteocyte-specific monoclonal antibody, and then observed by confocal laser scanning microscopy. The elastic modulus of living cells was analyzed with atomic force microscopy. To examine the consequences of focal adhesion formation on the elastic modulus, cells were pretreated with GRGDS and GRGES, and then the elastic modulus of the cells was analyzed. Focal adhesions in the cells were visualized by immunofluorescence of vinculin. From fluorescence images, we could distinguish osteoblasts, osteoid osteocytes and mature osteocytes both in vivo and in vitro. The elastic modulus of peripheral regions of cells in all three populations was significantly higher than in their nuclear regions. The elastic modulus of the peripheral region of osteoblasts was 12053+/-934 Pa, that of osteoid osteocytes was 7971+/-422 Pa and that of mature osteocytes was 4471+/-198 Pa. These results suggest that the level of elastic modulus of bone cells was proportional to the stage of changing from osteoblasts to osteocytes. The focal adhesion area of osteoblasts was significantly higher than that of osteocytes. The focal adhesion area of osteoblasts was decreased after treatment with GRGDS, however, that of osteocytes was not. The elastic modulus of osteoblasts and osteoid osteocytes were decreased after treatment with GRGDS. However, that of mature osteocytes was not changed. There were dynamic changes in the mechanical property of elastic modulus and in focal adhesions of bone cells.

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  • Comparison of the intrusion effects on the maxillary incisors between implant anchorage and J-hook headgear

    Toru Deguchi, Takashi Murakami, Shingo Kuroda, Toshinori Yabuuchi, Hiroshi Kamioka, Teruko Takano-Yamamoto

    AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS   133 ( 5 )   654 - 660   2008.5

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    Introduction: Recently, miniscrews have been used to provide anchorage during orthodontic treatment, especially for incisor intrusion. Miniscrews during incisor intrusion are commonly used in implant orthodontics. Traditionally, effective incisor intrusion has been accomplished with J-hook headgear. In this study, we compared the effect of incisor intrusion, force vector, and amount of root resorption between implant orthodontics and J-hook headgear. Methods: Lateral cephalometric radiographs from 8 patients in the implant group and 10 patients in the J-hook headgear group were analyzed for incisor retraction. The estimated force vector was analyzed in the horizontal and vertical directions in both groups. Root resorption was also measured on periapical radiographs. Results: In the implant group, significant reductions in overjet, overbite, maxillary incisor to palatal plane, and maxillary incisor to upper lip were observed after intrusion of the incisors. In the J-hook headgear group, significant reductions in overjet, overbite, maxillary incisor to upper lip, and maxillary incisor to SN plane were observed after intrusion of the incisors. There were significantly greater reductions in overbite, maxillary incisor to palatal plane, and maxillary incisor to upper lip in the implant group than in the J-hook headgear group. Estimated force analysis resulted in significantly more force in the vertical direction and less in the horizontal direction in the implant group. Furthermore, significantly less root resorption was observed in the implant group compared with the J-hook headgear group. Conclusions: The maxillary incisors were effectively intruded by using miniscrews as orthodontic anchorage without patient cooperation. The amount of root resorption was not affected by activating the ligature wire from the miniscrew during incisor intrusion.

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  • Hormonal, pH, and calcium regulation of connexin 43-mediated dye transfer in osteocytes in chick calvaria. Reviewed International journal

    Yoshihito Ishihara, Hiroshi Kamioka, Tadashi Honjo, Hirotaka Ueda, Teruko Takano-Yamamoto, Takashi Yamashiro

    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research   23 ( 3 )   350 - 60   2008.3

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    UNLABELLED: Gap junctional intercellular communication among osteocytes in chick calvaria, their natural 3D environment, was examined using FRAP analysis. Cell-cell communication among osteocytes in chick calvaria was mediated by Cx43 and was regulated by extracellular pH, extracellular calcium ion concentration, and PTH. INTRODUCTION: The intercellular network of communication among osteocytes is mediated by gap junctions. Gap junctional intercellular communication (GJIC) is thought to play an important role in integration and synchronization of bone remodeling. We hypothesized that extracellular pH (pH(o)) and extracellular calcium ion concentration ([Ca2+](e)), both of which are dynamically altered by osteoclasts during bone remodeling, affect GJIC among osteocytes. Using fluorescence replacement after photobleaching (FRAP) analysis, we examined the effect of changes in pH(o) and [Ca2+](e) and addition of PTH on GJIC in osteocytes in chick calvaria. Additionally, we examined the role of intracellular calcium on the regulation of GJIC among osteocytes. MATERIALS AND METHODS: Anti-Connexin43 (Cx43) immunolabeling was used to localize gap junctions in chick calvaria. GJIC among osteocytes in chick calvariae was assessed using FRAP. RESULTS: Cx43 immunoreactivity was detected in most of the osteocyte processes. FRAP analysis showed dye-coupling among osteocytes in chick calvariae. In untreated osteocytes, fluorescence intensity recovered 43.7 +/- 2.2% within 5 min after photobleaching. Pretreatment of osteocytes with 18 alpha-GA, a reversible inhibitor of GJIC, significantly decreased fluorescence recovery to 10.7 +/- 2.2%. When pH(o) was decreased from 7.4 to 6.9, fluorescence recovery significantly decreased from 43.3 +/- 2.9% to 19.7 +/- 2.3%. Conversely, when pH(o) was increased from 7.4 to 8.0, fluorescence recovery was significantly increased to 61.9 +/- 4.5%. When [Ca2+](e) was increased from 1 to 25 mM, fluorescence recovery was significantly decreased from 47.0 +/- 6.1% to 16.1 +/- 2.1%. In bone fragments exposed to 1.0-10 nM rPTH for 3 h, replacement of fluorescence was significantly increased to 60.7 +/- 7.2%. Chelating intracellular calcium ions affected GJIC regulation by [Ca2+](e) and PTH. CONCLUSIONS: Our study of cell-cell communication between osteocytes in chick calvaria showed for the first time that GJIC among osteocytes is regulated by the extracellular environment and by hormonal stimulation during bone remodeling. This method may be more biologically relevant to living bone than current methods.

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  • 岡山大学大学院顎顔面口腔矯正学分野における過去6年間の矯正相談の実態について

    菅原康代, 上岡 寛, 薮内利憲, 山城 隆

    岡山歯学会雑誌   27 ( 1 )   29 - 34   2008

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  • 過去10年間に岡山大学医学部・歯学部附属病院矯正歯科に来院した矯正患者の実態調査

    柳田 剛志, 上岡 寛, 本城 正, 山城 隆

    岡山歯学会雑誌   27 ( 1 )   35 - 42   2008

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  • 著しいオトガイ後退を伴う顎顔面の変形に対し、オトガイ形成術とベニアグラフトを併用した症例

    志茂 剛, 西山 明慶, 上岡 寛, 酒井 雄一, 川邉 紀章, 銅前 昇平, 小野 達生, 岸本 晃治, 目瀬 浩, 山城 隆, 佐々木 朗

    岡山歯学会雑誌   26 ( 2 )   147 - 151   2007.12

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    17歳女性。斜頸による舌骨上筋群および下筋群の高度緊張を認めた著しいオトガイ後退を伴う顎顔面の変形をきたした症例で、オトガイ部の後退に対する形態改善の目的で受診となった。顔貌所見にて正貌で眼瞼下垂、側貌でconvex type、口腔内所見にて両側Angel class II、overjet 3.7mm、overbite -2.5mm、前歯部は開咬を示していた。セファロ分析ではオトガイの劣成長を認め、舌骨が下方へ位置していた。CTデータをOsiriX Medical Imaging Softwareを用いた3Dおよび再構築CT画像においては、両側下顎犬歯から両側第二大臼歯部にかけて舌側皮質骨に溝状の凹みと同部の骨の非薄を認めた。骨非薄部の厚みは最も薄いところで約3mmであった。下顎後退症の臨床診断で、オトガイ形成術とベニアグラフトを併用して治療を行った。術後の側貌においてはlabiomental foldが明瞭化し、オトガイから頸部にかけての審美的改善が認められた。術後経過は良好で、骨片のずれや吸収等は認められない。

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  • Surgical orthodontic treatment for a patient with unilateral cleft lip and palate and temporomandibular disorder

    Hiroshi Kamioka, Yoshihito Ishihara, Masahide Inoue, Teruko Takano-Yamamoto

    Orthodontic Waves   66 ( 3 )   90 - 98   2007.9

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    The patient was a 12-year-old female who presented with unilateral cleft lip and palate, facial asymmetry, mandibular protrusion, resorption of condyle heads, and transverse maxillary deficiency. At the age of 13 years 10 months, maxillary expansion and autogenous bone graft were performed to improve the maxillary collapsed arch. Aged 14 years 10 months, she described symptoms of temporomandibular joint pain and dysfunction. Segmental Le Fort I osteotomy and intraoral vertical ramus osteotomy (IVRO) were performed at the age of 15 years 11 months. Segmental Le Fort I osteotomy resulted in improved sagittal and transverse discrepancy of the maxilla. After IVRO, facial symmetry, a good facial profile, and disappearance of TMD symptoms were achieved. The occlusion and esthetics were stable after 5 years of retention. © 2007 Elsevier Ltd and the Japanese Orthodontic Society.

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  • Erratum: Actin and microtubule cytoskeletons of the processes of 3D-cultured MC3T3-E1 cells and osteocytes (Journal of Bone and Mineral Metabolism (2007) 25, (151-158) DOI: 10.1007/s00774-006-0745-5) Reviewed

    Sakhr A. Murshid, Hiroshi Kamioka, Yoshihito Ishihara, Ryoko Ando, Yasuyo Sugawara, Teruko Takano-Yamamoto

    Journal of Bone and Mineral Metabolism   25 ( 4 )   259   2007.7

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  • A case of Stickler syndrome with orthodontic treatment

    Hiroshi Kamioka, Yoshihito Ishihara, Daisuke Sawada, Teruko Takano-Yamamoto

    Orthodontic Waves   66 ( 2 )   52 - 59   2007.6

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    At the age of 10 years and 10 months, a patient was brought to our office with a chief complaint of dental crowding and narrow maxilla. She had been diagnosed as having Pierre-Robin sequence at birth. She showed a convex profile with Class II molar relationship. The dental relationship was corrected with headgear and functional appliance in phase I orthodontic treatment. Optimal intercuspation of the teeth achieved after edgewise treatment and good facial profile with Class I relationship were obtained. When she started retention at the age of 17 years, she was diagnosed as having Stickler syndrome. Although slight reduction of the mandible was observed after a retention period of 5 years, optimal intercuspation of teeth was maintained. © 2007 Elsevier Ltd and the Japanese Orthodontic Society.

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  • Primary cultures of chick osteocytes retain functional gap junctions between osteocytes and between osteocytes and osteoblasts

    Hiroshi Kamioka, Yoshihito Ishihara, Hans Ris, Sakhr A. Murshid, Yasuyo Sugawara, Teruko Takano-Yamamoto, Soo-Siang Lim

    MICROSCOPY AND MICROANALYSIS   13 ( 2 )   108 - 117   2007.4

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    The inaccessibility of osteocytes due to their embedment in the calcified bone matrix in vivo has precluded direct demonstration that osteocytes use gap junctions as a means of intercellular communication. In this article, we report successfully isolating primary cultures of osteocytes from chick calvaria, and, using anti-connexin 43 immunocytochemistry, demonstrate gap junction distribution to be comparable to that found in vivo. Next, we demonstrate the functionality of the gap junctions by (1) dye coupling studies that showed the spread of microinjected Lucifer Yellow from osteoblast to osteocyte and between adjacent osteocytes and (2) analysis of fluorescence replacement after photobleaching (FRAP), in which photobleaching of cells loaded with a membrane-permeable dye resulted in rapid recovery of fluorescence into the photobleached osteocyte, within 5 min postbleaching. This FRAP effect did not occur when cells were treated with a gap junction blocker (18 alpha-glycyrrhetinic acid), but replacement of fluorescence into the photobleached cell resumed when it was removed. These studies demonstrate that gap junctions are responsible for intercellular communication between adjacent osteocytes and between osteoblasts and osteocytes. This role is consistent with the ability of osteocytes to respond to and transmit signals over long distances while embedded in a calcified matrix.

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  • Less response of osteocyte than osteoblast to mechanical force: implication of different focal adheison fometion

    Takano-YamamotoT, Kamioka H, Sugawara Y

    Interface Oral Health Science   1 ( 1 )   149 - 158   2007

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  • Actin and microtubule cytoskeletons of the processes of 3D-cultured MC3T3-E1 cells and osteocytes. Reviewed

    Sakhr A Murshid, Hiroshi Kamioka, Yoshihito Ishihara, Ryoko Ando, Yasuyo Sugawara, Teruko Takano-Yamamoto

    Journal of bone and mineral metabolism   25 ( 3 )   151 - 8   2007

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    Cell shape is the most critical determinant of cell function and is potentially influenced by the organization of a cell's cytoskeletal components. It has been reported that three-dimensionally cultured osteoblasts have a morphology that closely resembles that of osteocytes, most notably including formation of processes. We have previously shown the critical differences between cytoskeletal components in osteoblasts and osteocytes in two-dimensional culture. We have now extended that investigation to the cytoskeletal components of 3D-cultured osteoblasts and osteocytes using 3D cultures of the osteoblast cell line, MC3T3-E1, and primary osteocytes grown in collagen gel. Three-dimensional fluorescent image reconstructions for actin, fimbrin, alpha-actinin, myosin, tropomyosin, and microtubules were made using IMARIS software. Actin, fimbrin, alpha-actinin, myosin, and tropomyosin all appeared in the processes of both cell types, but fimbrin and myosin showed differences in their distribution patterns between cell types. Microtubules were limited in distribution to the proximal region of osteocyte processes but extended the entire length of MC3T3-E1 cell processes. Microtubules were essential for the integrity and formation of MC3T3-E1 cell processes, but osteocyte processes were dependent on actin. These results showed that there are significant differences between the actin and microtubule cytoskeletons in the processes of 3D-cultured MC3T3-E1 cells and in the processes of 3D-cultured primary osteocytes. These differences in the cytoskeleton of the processes of 3D-cultured osteoblasts and of osteocyte dendrites suggest that osteoblast processes may have a different functional role than the osteocyte dendritic network.

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  • alpha(V)beta(3) Integrin ligands enhance volume-sensitive calcium influx in mechanically stretched osteocytes

    Akimitsu Miyauchi, Masayuki Gotoh, Hiroshi Kamioka, Kohei Notoya, Hideki Sekiya, Yasuyuki Takagi, Yoshio Yoshimoto, Hitoshi Ishikawa, Kazuo Chihara, Teruko Takano-Yamamoto, Takuo Fujita, Yuko Mikuni-Takagaki

    JOURNAL OF BONE AND MINERAL METABOLISM   24 ( 6 )   498 - 504   2006.11

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    We propose that specific osteocyte-matrix interactions regulate the volume-sensitive calcium influx pathway, which we have shown is mediated by stretch-activated cation channels (SA-Cat) and is essential for the stretch-activated anabolic response in bone. The current study measured the hypotonic swelling-induced increase in cytosolic calcium concentration, [Ca2+](i), in rat osteocytes, and found that cells adherent to different matrices behave differently. Osteopontin and vitronectin, matrix molecules that bind the alpha(V)beta(3) integrin, induced larger responses to the hypotonic swelling than other matrix molecules that bind other integrins. Addition of echistatin, which is a soluble alpha(V)beta(3) ligand, significantly enhanced the hypotonic [Ca2+](i) increase in addition to inducing an immediate increase in [Ca2+](i) by itself. These results strongly support the contention that alpha(V)beta(3) integrin signaling in osteocytes interacts with that in mechanotransduction, which is downstream of SA-Cat.

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  • Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast: Implication of different focal adhesion formation

    Hiroshi Kamioka, Yasuyo Sugawara, Sakhr A. Murshid, Yoshihito Ishihara, Tadashi Honjo, Teruko Takano-Yamamoto

    JOURNAL OF BONE AND MINERAL RESEARCH   21 ( 7 )   1012 - 1021   2006.7

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    Introduction: To explore the immediate response to mechanical stress in a bone cell population, we examined flow-induced calcium transients. In addition, the involvement of focal adhesion-related calcium transients in response to fluid flow in the cells was studied.
    Materials and Methods: Bone cells were isolated from 16-day-old embryonic chicken calvaria by serial treatment with EDTA and collagenase. Single cells on glass without intercellular connections were subjected to fluid flow, and intracellular calcium concentration was measured using imaging with fluo-3. The identification of cell populations in the same field was performed with a chick osteocyte-specific antibody, OB7.3, and an alkaline phosphatase substrate, ELF-97, for osteoblast identification afterward. Immunofluorescence staining of vinculin was performed to visualize focal adhesions.
    Results: The percentage of cells responding to fluid shear stress at 1.2 Pa was 5.5% in osteocytes, 32.4% in osteoblasts, and 45.6% in OB7.3/ELF-97-negative cells. Furthermore, osteoblasts and OB7.3/ELF-97-negative cells were more responsive to 2.4 Pa than 1.2 Pa, whereas osteocytes were less responsive. The elevation of calcium transients over baseline did not show any significant differences in the populations. To elucidate the mechanism accounting for the fact that single osteocytes are less sensitive to fluid shear stress of up to 2.4 Pa than osteoblasts, we studied focal adhesion-related calcium transients. First, we compared focal adhesion formation between osteocytes and osteoblasts and found a larger number of focal adhesions in osteoblasts than in osteocytes. Next, when the cells were pretreated with GRGDS (0.5 mM) before flow treatment, a significant reduction of calcium transients in osteoblasts (18%) was observed, whereas calcium transients in osteocytes were not changed by GRGDS. Control peptide GRGES did not reduce the calcium transients in either cell type. Furthermore, we confirmed that osteoblasts in calvaria showed a marked formation of vinculin plaques in the periphery of the cells. However, osteocytes in calvaria showed faint vinculin plaques only at the base of the processes.
    Conclusions: On glass, single osteocytes are less sensitive to fluid shear stress up to 2.4 Pa than osteoblasts. The difference in calcium transients might be related to differences in focal adhesion formation. Shear stress of a higher magnitude or direct deformation may be responsible for the mechanical response of osteocytes in bone.

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  • Quantitative evaluation of cortical bone thickness with computed tomographic scanning for orthodontic implants

    Toru Deguchi, Miho Nasu, Kaoru Murakami, Toshinori Yabuuchi, Hiroshi Kamioka, Teruko Takano-Yamamoto

    American Journal of Orthodontics and Dentofacial Orthopedics   129 ( 6 )   721 - e12   2006.6

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    Introduction: The purpose of this study was to quantitatively evaluate cortical bone thickness in various locations in the maxilla and the mandible. In addition, the distances from intercortical bone surface to root surface, and distances between the roots of premolars and molars were also measured to determine the acceptable length and diameter of the miniscrew for anchorage during orthodontic treatment. Methods: Three-dimensional computed tomographic images were reconstructed for 10 patients. Cortical bone thicknesses were measured in the buccal and lingual regions mesial and distal to the first molar, distal to the second molar, and in the premaxillary region at 2 different levels. Differences in cortical bone thickness at 3 angles (30°, 45°, and 90°) were also assessed. Distances of the intercortical bone surface to the root surface and the root proximity were also measured at the above areas. Results: Significantly less cortical bone thickness was observed at the buccal region distal to the second molar compared with other areas in the maxilla. Significantly more cortical bone was observed on the lingual side of the second molar compared with the buccal side. In the mandible, mesial and distal to the second molar, significantly more cortical bone was observed compared with the maxilla. Furthermore, significantly more cortical bone was observed at the anterior nasal spine level than at Point A in the premaxillary region. Cortical bone thickness resulted in approximately 1.5 times as much at 30° compared with 90° Significantly more distance from the intercortical bone surface to the root surface was observed at the lingual region than at the buccal region mesial to the first molar. At the distal of the first mandibular molar, significantly more distance was observed compared to that in the mesial, and also compared with both distal and mesial in the maxillary first molar. There was significantly more distance in root proximity in the mesial area than in distal area at the first molar, and significantly more distance was observed at the occlusal level than at the apical level. Conclusions: These data show that the safest location for placing miniscrews might be mesial or distal to the first molar, and an acceptable size of the miniscrew is less than approximately 1.5 mm in diameter and approximately 6 to 8 mm in length. © 2006 American Association of Orthodontists.

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  • Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei

    H Ichikawa, T Yabuuchi, HW Jin, R Terayama, T Yamaai, T Deguchi, H Kamioka, T Takano-Yamamoto, T Sugimoto

    BRAIN RESEARCH   1081   113 - 118   2006.4

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    Immunohistochemistry for brain-derived neurotrophic factor (BDNF) was performed on the rat trigeminal ganglion (TG). The immunoreactivity (IR) was detected in 46% of TG neurons. These neurons were mostly small- or medium-sized (range, 149.7-1246.3 mu m(2); mean +/- SD = 373.4 +/- 151.6 mu m(2)). A double immunofluorescence method also revealed that 54% of BDNF-immunoreactive (IR) neurons were immunoreactive for calcitonin-gene related peptide. in addition, 93% of BDNF-IR TG neurons contained vanilloid receptor subtype 1. However, the co-expression of BDNF and vanilloid receptor 1-like receptor was very rare (less than 1%). In the trigeminal sensory nuclei, laminae II of the medullary dorsal horn was abundant in presumed BDNF-IR axon terminals. Such profiles were also detected in the dorsolateral part of the subnucleus oralis. The retrograde tracing and immunohistochemical methods demonstrated that BDNF-IR was common among cutaneous TG neurons (47%) but not tooth pulp TG neurons (13%). The present study indicates that BDNF-IR TG neurons have unmyelinated axons and project to the superficial medullary dorsal horn. It is likely that BDNF-containing neurons in both the trigeminal and spinal sensory systems have similarities in morphology and function. However, the content of BDNF in TG neurons probably depends on their peripheral targets. BDNF seems to convey nociceptive cutaneous input to the trigeminal sensory nuclei. (c) 2006 Elsevier B.V. All rights reserved.

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  • Implementation and evaluation of the debate-style tutrial study in a third-year dental curriculum in Japan.

    Shingaki R, Kamioka H, Irie M, Nishimura F

    International Education Journal   2006

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  • 英語原著論文を用いた岡山大学歯学部5年次生への教育について-アンケートからの考察-

    上岡 寛, 宮脇正一, 那須美穂, 山本照子

    日本歯科医学教育雑誌   2006

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  • Three-dimensional reconstruction of chick calvarial osteocytes and their cell processes using confocal microscopy

    Y Sugawara, H Kamioka, T Honjo, K Tezuka, T Takano-Yamamoto

    BONE   36 ( 5 )   877 - 883   2005.5

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    Osteocytes are surrounded by hard bone matrix. Therefore, it has not previously been possible to demonstrate the real architecture of the osteocyte network in bone. We previously reported that it is possible to observe osteocytes in bone by labeling the cells with fluorescence and using confocal laser scanning (CLS) microscopy. In this study, we for the first time conducted an extensive analysis of the morphology and morphometry of the three-dimensional (3D) osteocyte structure using three-dimensionally reconstructed fluorescent images. Sixteen-day-old embryonic chick calvariae were stained with fluorescently labeled phalloidin and observed using a confocal laser scanning microscope. Morphometry of osteocytes in the calvaria was analyzed using extensive three-dimensional reconstructing software IMARIS, process length measuring software NEURON TRACER and cell surface area-/cell volume-analyzing software SURPASS. From the IMARIS-derived images, we found that the average of 10 osteocytes is 52.7 &PLUSMN; 5.7 processes, and the point-to-point distance between centers of the osteocytes was 24.1 &PLUSMN; 2.8 &mu; m. In addition, we could calculate that each osteocyte spans an average of 4180 &PLUSMN; 673 &mu; m(3) of bone volume. NEURON TRACER showed that the length of osteocyte processes was 0.26 &PLUSMN; 0.02 &mu; m per 1 &mu; m(3) bone compartment. In addition, SURPASS indicated that the surface area of osteocytes was 0.36 &PLUSMN; 0.03 &mu; m(2) per 1 &mu; m(3) bone compartment and that the volume ratio of osteocyte cell body to bone compartment was 9.42% &PLUSMN; 1.18%. Together, the average total length of the processes, the average surface area, and the average volume of one osteocyte were 1070 &PLUSMN; 145 &mu; m, 1509 &PLUSMN; 113 &mu; m(2), and 394 &PLUSMN; 49 &mu; m(3), respectively. It is possible to reconstruct the real architecture of the osteocyte network and obtain morphometric data from fluorescently labeled osteocytes in chick calvaria. &COPY; 2004 Elsevier Inc. All rights reserved.

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  • 最近7年間に発表された英語原著論文を用いた岡山大学歯学部5年次生への英語教育について.

    上岡 寛, 宮脇正一, 出口 徹, 黒田晋吾, 福永智広, 湊 雅直, 菅原康代, 川邉紀章, 荒木佳子, 片山 朗, 山本照子

    岡山歯学会雑誌   2005

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  • Possible roles of Runx1 and Sox9 in incipient intramembranous ossification

    T Yamashiro, XP Wang, Z Li, S Oya, T Aberg, T Fukunaga, H Kamioka, NA Speck, T Takano-Yamamoto, Thesleff, I

    JOURNAL OF BONE AND MINERAL RESEARCH   19 ( 10 )   1671 - 1677   2004.10

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    We evaluated the detailed expression patterns of Runx1 and Sox9 in various types of bone formation, and determined whether Runx1 expression was affected by Runx2 deficiency and Runx2 expression by Runx1 deficiency. Our results indicate that both Runx1 and Sox9 are intensely expressed in the future osteogenic cell compartment and in cartilage. The pattern of Runx1 and Sox9 expression suggests that both genes could potentially be involved in incipient intramembranous bone formation during craniofacial development.

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  • Terminal differentiation of osteoblasts to osteocytes is accompanied by dramatic changes in the distribution of actin-binding proteins

    H Kamioka, Y Sugawara, T Honjo, T Yamashiro, T Takano-Yamamoto

    JOURNAL OF BONE AND MINERAL RESEARCH   19 ( 3 )   471 - 478   2004.3

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    Immunofluorescence staining of actin-binding proteins in osteoblasts and osteocytes was performed. alpha-Actinin, myosin, and tropomyosin showed similar organization in both osteoblastic stress fibers and osteocyte processes. However, fimbrin, villin, filamin, and spectrin showed dramatic differences in distribution between osteoblasts and osteocytes. This study suggested that terminal differentiation of osteoblasts to osteocytes is accompanied by highly dramatic changes in the distribution of actin-binding proteins.
    Introduction: We previously reported that osteocyte shape is dependent on actin filaments. To analyze the terminal differentiation from osteoblasts to osteocytes, we investigated the actin-binding proteins, which are the control elements in the dynamic organization of the actin cytoskeleton.
    Materials and Methods: We used primary chick osteocytes and osteoblasts, the phenotypes of which were confirmed by use of OB7.3, a chick osteocyte-specific monoclonal antibody and by detection of alkaline phosphatase activity, respectively, Immunofluorescence staining was performed for visualizing actin-binding proteins. Furthermore, we applied shear stress at 12 dyns/cm(2) to the cells and compared the changes in fimbrin distribution.
    Results: Immunofluorescence staining of fimbrin and alpha-actinin showed their presence in the processes of osteocytes, with especially strong signals of fimbrin at the sites of divarication of the processes. Anti-villin was reactive with the osteocyte cytoplasm but not with the processes. Interestingly, anti-villin immunoreactivity was much stronger in osteocytes than in osteoblasts. Filamin was localized along the stress fibers of osteoblasts but was seen only in those in the proximal base of osteocyte processes. Myosin and tropomyosin were found to have a similar pattern in both stress fibers of osteoblasts and osteocyte processes. The difference in the distribution of anti-spectrin staining was highly dramatic. Osteoblasts immunostained with anti-spectrin showed punctate signals on their cytoplasmic membranes, whereas anti-spectrin in osteocytes detected a filamentous organization; and the spectrin was totally colocalized with actin from the distal portion of the cytoplasmic processes to the cell center. In osteoblasts, shear stress induced recruitment of fimbrin to the end of stress fibers. However, fimbrin in the osteocyte processes did not change its localization.
    Conclusion: We found that terminal differentiation of osteoblasts to osteocytes was accompanied by highly dramatic changes in the distribution of actin-binding proteins, changes of which may affect cellular function.

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  • A surgical orthodontic case of anterior open bite developing during adolescence.

    Kamioka H, Honjo T, Takano-Yamamoto T

    Orthodontic Waves   2004

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  • 岡山大学医学部・歯学部附属病院矯正歯科における平成15年度歯科医師卒後臨床研修内容

    宮本学, 宮脇正一, 出口徹, 藤木辰哉, 福永智広, 黒田晋吾, 坪井佳子, 谷本裕子, 上岡寛, 山城隆, 山本照子

    岡山歯学会雑誌   23 ( 2 )   201 - 205   2004

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    平成15年度岡山大学医学部・歯学部附属病院歯科医師卒後臨床研修のベーシックプログラムに対する矯正歯科の関わりについて総括し,今後の課題について考察した.全人的な視点からさまざまな歯科治療分野に関わる適切で十分な医療情報を収集し,それらに基づいた治療計画を立案する能力を育成することは,総合歯科研修の大変重要な目的で,矯正歯科のような専門的な知識を基にした診断能力の育成も重要である.基本技術の育成を重要視しながら,こういった診断能力を育成していくことは,これからの社会に貢献できる歯科医師の育成にとって必須であることが示唆された

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  • Quantitative analysis of the effects of the undergraduate seminar: In the preparation for the national dental examining board.

    Deguchi T, Miyawaki S, Fujii A, Yabuuchi T, Kamioka H, Kuratani T, Takano-Yamamoto T

    Orthodontic Waves   2004

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  • Assessment of undergraduate orthodontic education: a correlation between written examination, laboratory work, and objective structured clinical examination (OSCE) of medical interviews.

    Miyawaki S, Deguchi T, Kamioka H, Fujiki T, Imai M, Yoshida T, Takano-Yamamoto T

    Orthodontic Waves   2004

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  • 岡山大学歯学部附属病院矯正科における週1回の診療時間の延長が新患数ならびに矯正治療を開始した患者数に及ぼす影響

    山本 照子, 宮脇 正一, 坪井 佳子, 谷本 裕子, 山城 隆, 上岡 寛, 宮本 学, 倉谷 豪, 藤木 辰哉, 黒田 晋吾, 出口 徹, 藤山 光治, 井上 雅秀, 大矢 伸治, 福永 智広, 森重 靖子, 本城 正, 荒木 佳子, 片山 朗, 菅原 康代, 石光 哲郎, 竹下 信郎, 湊 雅直, 酒井 雄一, 藤井 昭仁, 藪内 利憲

    岡山歯学会雑誌   22 ( 1 )   215 - 218   2003.6

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    平成14年2月より,矯正科において毎週水曜日の診療時間を2時間延長した.診療時間延長開始以降1年間の矯正科新患数は316名で,平成13年度は12年度とほぼ同数であったが,14年度は13年度に比較して11%増加した.14年度の矯正治療開始患者数は201名で,平成12年度は160名,13年度は177名であり,前年度に比較して14%増加した.診療時間延長は患者サービスに多大な貢献をしたことが示された

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  • Mechanical stimulation induces CTGF expression in rat osteocytes Reviewed

    T. Yamashiro, T. Fukunaga, N. Kobashi, H. Kamioka, T. Nakanisni, M. Takigawa, T. Takano-Yamamoto

    Journal of Dental Research   80 ( 2 )   461 - 465   2001

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    Connective tissue growth factor (CTGF), which is encoded by an immediate early gene and a member of the CCN family, has been shown to be expressed in osteoblasts, fibroblasts, and chondrocytes. Although CTGF is expressed in bone and cartilage tissues, we tested the hypothesis that CTGF is regulated in mechanotransduction. In the alveolar bone during experimental tooth movement, CTGF mRNA was expressed in osteoblasts and in osteocytes localized around the periodontal ligament under control conditions. Interestingly, 12 hrs after the start of experimental tooth movement, the expression of CTGF mRNA in osteocytes and osteoblasts became more intense around the periodontal ligament, and the intense expression of CTGF extended to osteocytes situated deep in alveolar bone matrix apart from periodontal ligament in both tension and compression sides. Our present findings indicate that CTGF could play a role in regulation of osteocyte function during the mechanical stimulation of bone.

    DOI: 10.1177/00220345010800021201

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  • A three-dimensional distribution of osteocyte processes revealed by the combination of confocal laser scanning microscopy and differential interference contrast microscopy Reviewed

    H. Kamioka, T. Honjo, T. Takano-Yamamoto

    Bone   28 ( 2 )   145 - 149   2001

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    Osteocytes are the most numerous cells in bone, embedded within the mineralized bone matrix. Their slender cytoplasmic processes form a complex intercellular network. In addition, these processes are thought to be important structures in the response to mechanical stress. This study provides an extensive analysis of the three-dimensional structure of the osteocyte and its processes in 16-day-old embryonic chick calvariae, based on nondestructive subsurface histotomography using both confocal laser scanning (CLS) microscopy and differential interference contrast (DIC) microscopy. OB7.3, a chicken osteocyte-specific monoclonal antibody, and Texas Red-X-conjugated phalloidin were used to confirm the osteocyte phenotype and to identify whole cells in the calvariae, respectively. Serial CLS images revealed morphological changes in bone cells up to 20 μm in depth. Osteocytes had widely spread their processes into the osteoblast layer, and we found for the first time that some of these processes had elongated to the vascular-facing surface of the osteoblast layer. Furthermore, stereotype images reconstructed from CLS images could show the three-dimensional distribution of these processes. Using the stereopair image, we could evaluate the frequency of processes between osteocytes and osteoblasts. Complementation of DIC microscopy revealed canaliculi and lacunae with high contrast. The distributional pattern of canaliculi generally coincided with that of the osteocyte processes. We consider that the combination method of CLS microscopy and DIC microscopy using a laser scanning microscope is a very useful new technical approach for investigating osteocytes in bone. Copyright © 2001 Elsevier Science Inc.

    DOI: 10.1016/S8756-3282(00)00421-X

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  • Osteocyte Shape Is Dependent on Actin Filaments and Osteocyte Processes Are Unique Actin-Rich Projections Reviewed

    Kayo Tanaka-Kamioka, Hiroshi Kamioka, Hans Ris, Soo-Siang Lim

    Journal of Bone and Mineral Research   13 ( 10 )   1555 - 1568   1998.10

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    DOI: 10.1359/jbmr.1998.13.10.1555

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  • Extracellular calcium causes the release of calcium from intracellular stores in chick osteocytes Reviewed

    Hiroshi Kamioka, Yoshiki Miki, Koji Sumitani, Kahori Tagami, Kunihiro Terai, Kazuo Hosoi, Terushige Kawata

    Biochemical and Biophysical Research Communications   212 ( 2 )   692 - 696   1995.7

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    We have recently demonstrated that a rise in the extracellular divalent cation concentration induces a rapid elevation of cytosolic calcium in chick osteocytes. Here, we demonstrate that cytosolic calcium elevation that occurs in osteocytes on exposure to elevated extracellular calcium is independent of membrane voltage and is insensitive to modulation by organic calcium channel modulators, namely, BAY K 8644, nicardipine, and nifedipine. However, the calcium elevation was sensitive to modulation by an intracellular calcium antagonist, TMB-8, suggesting that the cytosolic calcium elevation was due to mobilization of this cation from an intracellular store. © 1995 Academic Press, Inc.

    DOI: 10.1006/bbrc.1995.2024

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  • Effects of transforming growth factor-β1 on formation and activation of osteoclasts Reviewed

    Hiroshi Kamioka, Keishi Ishikawa, Kayo Tanaka, Takuya Sato, Ken-Ichi Tezuka, Kenji Hiura, Koji Sumitani, Yoshiyuki Hakeda, Terushige Kawata, Masayoshi Kumegawa

    Journal of Bone and Mineral Metabolism   13 ( 1 )   3 - 9   1995.3

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    Transforming growth factor-β1 (TGF-β1) has biological functions in various types of cells. However, its roles in the regulation of osteoclast formation and function are unclear. To examine them, we employed a culture system in which unfractionated cells obtained from long bones of 13-day-old mice were cultured on a dentine slice. We found that TGF-β1 has a potent inhibitory effect on osteoclastic bone resorption at a dose of 0.2-5 ng/ml. By electron microscopy the osteoclasts appeared to have fewer mitochondria and ruffled borders than those in control cultures. But in the presence of 1,25-dihydroxyvitamin D3, [1,25-(OH)2D3], TGF-β1 at a dose of 0.2-1 ng/ml stimulated the formation of osteoclasts from unfractionated bone cell cultures in which preexistent osteoclasts had degenerated. Thus, using stromal cell-free he-mopoietic blast cells, we examined the direct action of TGF-β1 on osteoclast precursors. Although TGF-β1 inhibited tartrate-resistant acid phosphatase-positive (TRAP) multinucleate cell (MNC) formation induced by 1,25-(OH)2D3, the conditioned medium (CM) of TGF-β1-treated MC3T3-E1 cells stimulated such formation. These results suggest that TGF-β1 inhibits osteoclastic bone resorption but stimulates osteoclast formation via the action of factor(s) produced by TGF-β1-treated osteoblasts in the presence of 1,25-(OH)2D3. © 1995 Springer-Verlag.

    DOI: 10.1007/BF01771798

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  • Divalent cations elevate cytosolic calcium of chick osteocytes Reviewed

    Hiroshi Kamioka, Koji Sumitani, Kahori Tagami, Yoshiki Miki, Kunihiro Terai, Yoshiyuki Hakeda, Masayoshi Kumegawa, Terushige Kawata

    Biochemical and Biophysical Research Communications   204 ( 2 )   519 - 524   1994.10

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    Though it is proposed that osteocytes have several functions in bone metabolism, only limited definite information including results of calcium regulation has been forthcoming. We demonstrate here for the first time that the concentration of divalent cations, such as calcium, nickel and cadmium, directly regulates the cytosolic calcium concentration in osteocytes. © 1994 Academic Press. All rights reserved.

    DOI: 10.1006/bbrc.1994.2490

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  • The mechanisms and regulation of procathepsin L secretion from osteoclasts in bone resorption Reviewed

    Kahori Tagami, Hisao Kakegawa, Hiroshi Kamioka, Koji Sumitani, Terushige Kawata, Brigita Lenarcič, Vito Turk, Nobuhiko Katunuma

    FEBS Letters   342 ( 3 )   308 - 312   1994.4

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    The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppressed by the addition ofcalcitonin. The Z-Phe-Arg-MCA hydrolysing activity increased in the medium through the effect of PTH is considered to be a kind of procathepsin L by Western blotting analysis, and was suppressed by calcitonin addition. Furthermore, monensin inhibited not only the PTH-induced pit formation, but also cysteine proteinase activity in osteoclasts. Therefore, the procathepsin L excreted might be transferred from endothelial reticulum via Golgi and/or via lysosomes. © 1994.

    DOI: 10.1016/0014-5793(94)80522-9

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  • Molecular cloning of a possible cysteine proteinase predominantly expressed in osteoclasts Reviewed

    Ken-Ichi Tezuka, Yoko Tezuka, Akiko Maejima, Takuya Sato, Ken Nemoto, Hiroshi Kamioka, Yoshiyuki Hakeda, Masayoshi Kumegawa

    Journal of Biological Chemistry   269 ( 2 )   1106 - 1109   1994.1

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    Osteoclasts are multinucleate giant cells playing key roles in bone resorption. These cells solubilize mineralized bone matrix by means of acid and protease action
    however, the precise mechanism of this process is not well known. Recently, we succeeded in the isolation of pure osteoclasts from rabbit bones and constructed a cDNA library. Using a differential screening procedure, two genes expressed predominantly in osteoclasts compared with spleen cells were isolated (Tezuka, K., Sato, T., Kamioka, H., Nijweide, P. J., Tanaka, K., Matsuo, T., Ohta, M., Kurihara, N., Hakeda, Y., and Kumegawa, M. (1992) Biochem. Biophys. Res. Commun. 186, 911-917). One of them, OC-2, was found to encode a possible cysteine proteinase structurally related to cathepsins L and S. By in situ hybridization, OC-2 was confirmed to be expressed in osteoclasts in vivo. By Northern blot analysis, OC-2 was highly and preferentially expressed in osteoclasts compared with other tissues such as kidney, liver, spleen, and lung. The predominant expression of OC-2 in osteoclasts may suggest that OC-2 encodes a protein, possibly a cysteine proteinase, that plays an important role in osteoclastic bone resorption.

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  • A simple method of isolation of rabbit osteoclasts Reviewed

    Sato T, Kamioka H, Nemoto K, Tezuka K, Tanaka K, Sugimoto T, Kaji H, Miyauchi A, Yoshizawa K, Yamaguchi Y, Hakeda Y, Kumegawa M

    Dentistry in Japan   31   3 - 8   1994.1

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  • Participation of cathepsin L on bone resorption Reviewed

    Hisao Kakegawa, Takeshi Nikawa, Kahori Tagami, Hiroshi Kamioka, Koji Sumitani, Terushige Kawata, Marinka Drobnič-Kosorok, Brigita Lenarčič, Vito Turk, Nobuhiko Katunuma

    FEBS Letters   321 ( 2-3 )   247 - 250   1993.4

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    The proteinase responsible for bone collagen degradation in osteo-resorption was examined. The bone pit formation induced by parathyroid hormone (PTH) was markedly suppressed by leupeptin, E-64 and cystatin A, while no inhibition was observed by CA-074, a specific inhibitor of cathepsin B. Pig leucocyte cysteine proteinase inhibitor (PLCPI), a specific inhibitor of cathepsin L, and chymostatin, a selective inhibitor of cathepsin L, completely inhibited the pit formation. Cathepsin L activity in osteoclasts was much higher than the other cathepsin activities. Serum calcium in rats placed on a low calcium diet was decreased by treatment of E-64 or cystatin A, but not by CA-074. These findings suggest that cathepsin L is the main proteinase responsible for bone collagen degradation. © 1993.

    DOI: 10.1016/0014-5793(93)80118-E

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  • Identification of osteopontin in isolated rabbit osteoclasts Reviewed

    Ken-ichi Tezuka, Takuya Sato, Hiroshi Kamioka, Peter J. Nijweide, Kayo Tanaka, Tetsu Matsuo, Mitsue Ohta, Noriyoshi Kurihara, Yoshiyuki Hakeda, Masayoshi Kumegawa

    Biochemical and Biophysical Research Communications   186 ( 2 )   911 - 917   1992.7

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    Bone remodeling is a complex process coupling bone formation and resorption. Osteoblasts, the bone-forming cells, are known to produce various bone matrix proteins and cytokines
    however, little is known about protein factors produced by osteoclasts or bone-resorbing cells. A method utilizing the high affinity of osteoclasts for tissue culture dishes was developed to isolate a large number of pure osteoclasts from rabbit long bones. A cDNA library was then constructed from these isolated osteoclasts, and differential cDNA screening was performed between osteoclasts and spleen cells. Two clones representing osteoclast-specific clones, named OC-1 and OC-2, were isolated. By Northern blot analysis, OC-1 was expressed in osteoclasts and in kidneys, whereas OC-2 was specific for osteoclasts. OC-1 was found to encode osteopontin from its nucleotide sequence, and therefore, osteopontin may have other functions for osteoclastic bone resorption besides osteoclast attachment to bone. © 1992.

    DOI: 10.1016/0006-291X(92)90832-6

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  • 1.25 dihydroxyvitamin D3 receptor exist of osteoclast precursors

    Kusuda M, Ishizuka S, Sumitani K, Hiura K, Kamioka H, Ishikawa K, Nii Y, Tagami K, Hakeda Y, Kumegawa M

    Dentitry in Japan   28   185 - 189   1991

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Books

  • 歯科矯正用アンカースクリューを用いた矯正歯科治療アトラス

    上岡寛, 中村政裕, 原規子, 片岡伴記, 川邉紀章, 石本和也, 出射明美( Role: Joint author)

    2019.11 

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  • 歯科矯正学

    飯田, 順一郎, 葛西, 一貴, 後藤, 滋巳, 末石, 研二, 槇, 宏太郎, 山城, 隆(歯学)( Role: Joint author ,  三章 成長発育 成長発育概論)

    医歯薬出版  2019.1  ( ISBN:9784263458327

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    Total pages:xviii, 436p   Language:Japanese

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  • 骨・臓器ネットワークとオステオサイト

    石原嘉人, 上岡 寛(骨細胞の単離と生骨中骨細胞のバイオイメージングを用いた解析)

    メディカルレビュー社  2016.2 

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  • 骨ペディア

    上岡 寛( Role: Joint author ,  骨細胞)

    羊土社  2015.5 

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  • 骨ペディア

    菅原康代, 上岡 寛( Role: Joint author ,  歯科矯正治療における骨代謝)

    羊土社  2015.5 

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  • 骨細胞の機能イメージング

    THE BONE  2014 

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  • Interface Oral Health Science 2007

    Springer  2007 

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  • 動物細胞培養マニュアル 瀬野悍二, 小山秀機, 黒木登志夫編集

    共立出版  2004 

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  • 骨形成と骨吸収 第2巻 須田立雄編集

    廣川書店  1995 

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MISC

  • 運動を支える骨と細胞の役割 Invited

    上岡 寛, 橋本真奈

    体育の科学   71 ( 7 )   501 - 505   2021.7

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  • 人骨が語るヒトと社会の変化 Invited

    瀬口典子, 上岡 寛

    科学   91 ( 2 )   186 - 191   2021.2

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  • 骨組織の3次元解析 Reviewed

    橋本真奈, 上岡 寛

    顕微鏡   53 ( 3 )   123 - 126   2018

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  • 歯が移動するために骨中で何が行われているのか?

    上岡 寛

    中・四国矯正歯科学会雑誌   28 ( 1 )   17 - 22   2016.8

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  • 口唇裂・口蓋裂患者に対する包括的チームアプローチについて

    上岡寛, 飯田征二, 木股敬裕

    日本遺伝カウンセリング会誌   37 ( 3 )   93 - 98   2016.3

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  • 歯の移動と骨細胞の役割

    上岡寛

    Clinical Calcium   25 ( 10 )   138 - 144   2015.10

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  • 720 Deformation simulation of osteocyte cell processes using image-based model of bone canaliculi

    YAMAMOTO Ryuta, KAMEO Yoshitaka, OOTAO Yoshihiro, ISHIHARA Masayuki, KAMIOKA Hiroshi, ADACHI Taiji

    2014 ( 89 )   "7 - 20"   2014.3

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  • 骨細胞の機能イメージング

    石原嘉人, 上岡 寛

    The bone   28 ( 2 )   73   2014

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  • [Mechanosensitivity of osteocytes]. Reviewed

    Hiroshi Kamioka, Takashi Yamashiro

    Clinical calcium   22 ( 5 )   697 - 704   2012.5

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    It is suggested that osteocytes play an important role in bone metabolism, especially in their regulation by mechanical stimuli. However, it has been still discussed how the osteocytes were activated by mechanical stimuli. Recently, theories based on fluid flow-induced shear stress stimulation around osteocytes in bone have appeared with the most prominence. In addition, we would like to introduce several theories to mechanically stimulate osteocyte, such as deformation, strain amplification by radial hoop strains, flow induced integrin mediated signaling, and primary cilia mediated signaling. To validate the availability of these theories, it is important to obtain 3-dimensional morphometrical data of osteocytes as well as their interaction with pericellular matrix.

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    Other Link: http://orcid.org/0000-0002-4419-9643

  • 【Osteocyte〜骨の司令塔としての役割〜】骨細胞による機械的刺激の感知

    上岡 寛, 山城 隆

    Clinical Calcium   22 ( 5 )   697 - 704   2012.4

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    多くの器官、組織、細胞は少なからずの影響を機械的刺激によって受ける。一見、静的にみえる骨も機械的刺激に応答して経時的にマクロな変化を遂げる。その結果は、骨に存在する細胞群が引き起こすミクロな変化の集積である。このような変化を生じるための機械的刺激センサーとして、骨細胞が注目されている。しかしながら、骨細胞は骨基質に囲まれている特異な環境のために、実際にどのようにして機械的刺激を感知しているのかは明らかではない。そこで、近年、生体工学的な試みにより、さまざまな骨細胞の機械的刺激の受容機構が提案されてきた。この総説では、これらの説が生まれた経緯を簡単に紹介するとともに、骨細胞およびその周囲骨基質の3次元構造をナノレベルで追求することの重要性について解説していきたい。(著者抄録)

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  • 超高圧電子顕微鏡トモグラフィーの生物試料への応用

    上岡 寛, 藤田一郎, 小賀智文, 岡本嗣久, 梶村直子, 西田倫希, 長谷川紀昭, 鷹岡昭夫

    NanotechJapan Bulletin (Web版)   4 ( 3 )   2011

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  • 骨細胞の3次元形態解析

    上岡 寛, 山城 隆

    THE BONE   24 ( 1 )   3 - 7   2010.1

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  • Peripheral administration of botulinum toxin type A decreases exaggerated neurotransmitter release and reverses neuropathy-induced gene expression changes in trigeminal ganglia Reviewed

    Ai Kumada, Yoshizo Matsuka, Igor Spigelman, Yoshihito Ishihara, Yumiko Yamamoto, Takayuki Hikasa, Wataru Sonoyama, Hiroshi kamioka, Takashi Yamashiro, Takuo Kuboki, Keiji Oguma

    http://f1000.com/posters/browse/summary/87   2010

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  • S0201-2-5 単離骨系細胞間におけるカルシウム応答伝播の非対称性(マイクロ・ナノバイオメカニクス:細胞生物学への接近(2)刺激と応答) Reviewed

    平 圭佑, 青沼 有紀, 安達 泰治, 北條 正樹, 上岡 寛

    年次大会講演論文集 : JSME annual meeting   2009 ( 5 )   21 - 22   2009.9

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  • Calcium response in single osteocytes to locally applied mechanical stimulus: differences in cell process and cell body. Reviewed International journal

    Taiji Adachi, Yuki Aonuma, Mototsugu Tanaka, Masaki Hojo, Teruko Takano-Yamamoto, Hiroshi Kamioka

    Journal of biomechanics   42 ( 12 )   1989 - 95   2009.8

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    It is proposed that osteocytes embedded in the bone matrix have the ability to sense deformation and/or damage to the matrix and to feed these mechanical signals back to the adaptive bone remodeling process. When osteoblasts differentiate into osteocytes during the bone formation process, they change their morphology to a stellate form with many slender processes. This characteristic cell shape may underlie the differences in mechanosensitivity between the cell processes and cell body. To elucidate the mechanism of cellular response to mechanical stimulus in osteocytes, we investigated the site-dependent response to quantitatively controlled local mechanical stimulus in single osteocytes isolated from chick embryos, using the technique of calcium imaging. A mechanical stimulus was applied to a single osteocyte using a glass microneedle targeting a microparticle adhered to the cell membrane by modification with a monoclonal antibody OB7.3. Application of the local deformation induced calcium transients in the vicinity of the stimulated point and caused diffusive wave propagation of the calcium transient to the entire intracellular region. The rate of cell response to the stimulus was higher when applied to the cell processes than when applied to the cell body. In addition, a large deformation was necessary at the cell body to induce calcium transients, whereas a relatively small deformation was sufficient at the cell processes, suggesting that the mechanosensitivity of the cell processes was higher than that of the cell body. These results suggest that the cell shape with slender processes contributes to the site-dependent mechanosensitivity in osteocytes.

    DOI: 10.1016/j.jbiomech.2009.04.034

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  • Site-Dependence of Mechanosensitivity in Isolated Osteocytes

    Y. Aonuma, T. Adachi, M. Tanaka, M. Hojo, T. Takano-Yamamoto, H. Kamioka

    13TH INTERNATIONAL CONFERENCE ON BIOMEDICAL ENGINEERING, VOLS 1-3   23 ( 1-3 )   2000 - +   2009

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    It is proposed that osteocytes embedded in mineralized bone matrix play a role in mechanosensory system during bone remodeling for mechanical adaptation. Various experimental and computer simulation approaches have revealed that the osteocytes sense the mechanical stimulus such as deformation/force and microdamages in bone matrix generated by mechanical loading, and also still have continued discussion. In this study, for interest to morphological character of osteocytes, we investigated site-dependence of mechanosensitivity in the osteocytes through observation of calcium response to applied local deformation. We developed a quantitative method using a microneedle and microparticles to apply local deformation to an isolated chick osteocyte, and analyzed intracellular calcium dynamics to the applied deformation. As the results, the applied local deformation induced calcium response at the vicinity of the stimulated point and caused its diffusive wave propagation to whole intracellular region in a single osteocyte. Furthermore, the large deformation was necessary at the cell body to induce calcium response, while a relatively small deformation was sufficient at the cell process, suggesting that the mechanosensitivity at the cell process was higher than that at the cell body. These results suggest that the cell shape with processes contributes to the mechanism of cellular response to mechanical stimulus in osteocytes, and that the site-dependent mechanosensitivity depends on cell shape in a single osteocyte.

    DOI: 10.1007/978-3-540-92841-6_498

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  • 骨細胞とメカニカルストレス

    上岡 寛, 山城 隆

    月刊 Clinical Calcium (医薬ジャーナル社)   18 ( 9 )   55 - 61   2008

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  • メカニカルセンサーとしての骨組織

    上岡 寛, 山城 隆

    月刊 メビオ   25 ( 8 )   44 - 53   2008

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  • 骨細胞における機械的刺激への応答能について

    上岡 寛

    季刊 腎と骨代謝   21 ( 3 )   191 - 198   2008

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  • 骨組織での細胞ー基質間および細胞ー細胞間の微細構造の3次元解析

    上岡 寛, ロメル, G, バカバック, アスドリッド, D, バッカー, ヤネカ クレインーヌレンド

    阪大複合機能ナノファウンダリ研究成果報告書 第2巻   2   56 - 57   2008

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  • メカニカルストレスと骨~骨細胞の生物学から骨リモデリングシミュレーションへ~

    手塚 建一, 岐阜大学大学院医学系研究科組織, 器官形成分野, 上岡 寛, 岡

    医学のあゆみ   221   76 - 80   2007.4

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  • 混合歯列期の矯正治療

    上岡 寛

    歯界月報(兵庫県歯科医師会)   ( 671号 )   45 - 49   2007

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  • 骨微細構造を反映した仮想骨の作成

    上岡 寛

    阪大複合機能ナノファウンダリ研究成果報告書(2007)   1   57 - 58   2007

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  • 骨細胞の機能と力学負荷による骨代謝調節

    手塚建一, 上岡 寛

    医学のあゆみ   221(1)76-80   2007

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  • 骨微細構造を反映した仮想骨の作成

    上岡 寛, 手塚健一, 山本照子

    平成18年大阪大学超高圧電子顕微鏡センター年報   82-83   2006

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  • 骨細胞における機械的刺激への応答能について

    上岡 寛, 菅原康代, Murshid A Sakhr, 石原嘉人, 本城正, 山本照子

    生体医工学雑誌 解説特集   44,4,484-489   2006

  • Mineralized Tissue

    上岡 寛

    Newsletter for JADR   ( 2 )   11 - 11   2006

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  • 骨組織における骨細胞の役割

    上岡寛, 久米川正好

    実験医学   13 ( 4 )   36 - 40   1995

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  • 破骨細胞の形成に対する骨芽細胞株(MC3T3-E1)の 作用

    日浦賢治, 上岡寛, 石川啓詞, 楠田倫紀, 新居泰浩, 住谷光治, 河田照茂

    歯界展望   79 ( 5 )   1246 - 1246   1992.5

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  • 骨形成と破骨細胞機能

    上岡寛, 久米川正好

    診断と治療   80 ( 7 )   1193 - 1197   1992

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Presentations

  • フッ化ナトリウムによるCCNファミリー遺伝子制御を介した歯肉線維化抑制作用の検討

    水川朋美、西田 崇、明石 翔、大杉綾花、大森一弘、中山真彰、高柴正悟、上岡 寛、滝川正春、久保田聡

    第42回岡山歯学会  2021.11.28  岡山歯学会

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    Event date: 2021.11.28

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山市   Country:Japan  

  • 喫煙によるAhRリガンドB[a]Pを介した破骨細胞分化および骨粗しょう症発症メカニズムの解明

    竹永紘子, 井澤 俊, 吉川友里, 浜田勇作, 王 紫儀, 上岡 寛

    第80回日本矯正歯科学会学術大会  2021.11.5 

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    Event date: 2021.11.3 - 2021.11.5

    Language:Japanese   Presentation type:Poster presentation  

  • 内因性AhRリガンドFICZによるCyp1a1を介した破骨細胞分化および骨代謝調節機構の解析

    吉川友理, 井澤 俊, 浜田勇作, 竹永紘子, 王 紫儀, 上岡 寛

    第80回日本矯正歯科学会学術大会  2021.11.5 

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    Event date: 2021.11.3 - 2021.11.5

    Language:Japanese  

    Country:Japan  

  • 軟骨細胞におけるRFX1を介したCCN3の発現制御機構の解明

    水川朋美、西田 崇、明石 翔、河田かずみ、菊池 菫、川木晴美、滝川正春、上岡 寛、久保田聡

    第94回日本生化学会大会  日本生化学会

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    Event date: 2021.11.3 - 2021.11.5

    Language:Japanese  

    Country:Japan  

  • 内分泌撹乱物質AhRリガンドはCyp1a1シグナルを介して破骨細胞分化および骨代謝を制御する

    吉川友理, 井澤俊, 浜田勇作, 竹永紘子, 王紫儀, 上岡寛

    第39回日本骨代謝学会学術集会  2021.10.9  日本骨代謝学会

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    Event date: 2021.10.8 - 2021.10.10

    Language:Japanese  

    Country:Japan  

  • 軟骨細解糖阻害剤NaFの線維化抑制効果とCCNファミリー遺伝子の関与

    水川朋美, 西田 崇, 明石 翔, 堀 彩花, 高柴正悟, 上岡 寛, 滝川正春, 久保田聡

    第62回日本生化学会 中国・四国支部例会  2021.9.10  日本生化学会 中国・四国支部

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    Event date: 2021.9.10 - 2021.9.11

    Language:Japanese  

  • 軟骨細胞でのRFX1によるCCNファミリータンパク質3遺伝子制御メカニズム

    水川朋美, 西田 崇, 明石 翔, 河田かずみ, 菊池 菫, 川木晴美, 滝川正春, 上岡 寛, 久保田聡

    第12回日本CCNファミリー研究会  2021.9.4  日本CCNファミリー研究会

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    Event date: 2021.9.4

    Language:Japanese  

    Venue:岡山市   Country:Japan  

  • 内分泌撹乱物質AhRリガンドはCyp1a1シグナルを介して破骨細胞分化および骨代謝を制御する

    吉川友理, 井澤俊, 浜田勇作, 竹永紘子, 王紫儀, 上岡寛

    第41回日本骨形態計測学会  2021.7.3 

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    Event date: 2021.7.1 - 2021.7.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 症候群性頭蓋骨縫合早期癒合症のチーム医療における矯正歯科医の役割について

    上岡 寛

    17th Oraniosynostosis 研究会  2021.6.26 

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    Event date: 2021.6.26

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:サン・ピーチOKAYAMA   Country:Japan  

  • 軟骨細胞における解糖系によるCCN3遺伝子発現制御メカニズム

    水川朋美, 西田 崇, 明石 翔, 上岡 寛, 滝川正春, 久保田聡

    第33回日本軟骨代謝学会  2021.3.26  日本軟骨代謝学会

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    Event date: 2021.3.26 - 2021.3.27

    Language:Japanese  

    Country:Japan  

  • 軟骨細胞におけるエネルギー代謝不全時でのCCN3増産システムの解明

    水川朋美, 西田 崇, 明石 翔, 上岡 寛, 滝川正春, 久保田聡

    第38回日本骨代謝学会学術集会  2020.10.10  日本骨代謝学会

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    Event date: 2020.10.9 - 2020.10.11

    Language:Japanese  

    Country:Japan  

  • Crosstalk between cytokine RANKL and AhR signaling in osteoclasts controls bone homeostasis International conference

    Takashi Izawa, Yuri Yoshikawa, Naozumi Ishimaru, Hiroshi Kamioka

    The 9th International Orthodontic Congress  2020.10.4 

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    Event date: 2020.10.4 - 2020.10.7

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  • Regulation of CCN3 gene expression by glycolytic activity in chondrocytes International conference

    Mizukawa T, Nishida T, Akashi S, Kamioka H, Takigawa M, Kubota S

    The 9th International Orthodontic Congress 

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    Event date: 2020.10.4 - 2020.10.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 軟骨細胞での解糖活性によるCCN3遺伝子の発現調節

    水川朋美, 西田 崇, 明石 翔, 掘 綾花, 高柴正悟, 上岡 寛, 滝川正春, 久保田聡

    第61回日本生化学会;中国;四国支部例会  2020.5.23  日本生化学会;中国;四国支部

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    Event date: 2020.5.23 - 2020.5.24

    Language:Japanese  

    Country:Japan  

  • 癒合不全により再固定を行ったObwegeser第II法症例の長期経過観察

    西山 明慶, 吉岡 徳枝, 伊原木 聰一郎, 田中 敦子, 小田垣 直弥, 川邊 紀章, 上岡 寛, 佐々木 朗

    日本顎変形症学会雑誌  2020.5  (NPO)日本顎変形症学会

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  • 上顎骨側方骨延長術後に上顎骨前方骨延長術を施行し良好な咬合関係が得られた唇顎口蓋裂の1例

    藤田 佑貴, 松村 達志, 中村 政裕, 有村 友紀, 岡本 成美, 山城 隆, 上岡 寛, 飯田 征二

    日本口蓋裂学会雑誌  2020.5  (一社)日本口蓋裂学会

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    Event date: 2020.5

    Language:Japanese  

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  • 上下顎歯列の三次元的な変形を認める上顎前突症例に顎矯正手術を行った1例

    河野 加奈, 川邉 紀章, 吉岡 徳枝, 西山 明慶, 佐々木 朗, 上岡 寛

    日本顎変形症学会雑誌  2020.5  (NPO)日本顎変形症学会

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    Event date: 2020.5

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  • フッ素イオンによるCCNファミリー遺伝子の制御

    水川 朋美, 西田 崇, 明石 翔, 堀 彩花, 高柴 正悟, 上岡 寛, 滝川 正春, 久保田 聡

    岡山歯学会雑誌  2019.12  岡山歯学会

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    Event date: 2019.12

    Language:Japanese  

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  • 二度の骨延長術を行った唇顎口蓋裂症例の長期保定報告

    中村 政裕, 松村 達志, 飯田 征二, 山城 隆, 上岡 寛

    日本矯正歯科学会大会プログラム・抄録集  2019.11  (公社)日本矯正歯科学会

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  • 下顎枝垂直骨切り術後に偏位側顎関節の非復位性関節円板前方転位の改善を認めた顔面非対称症例の一治験例

    植田 紘貴, 国富 陽介, 岡 直毅, 村上 隆, 佐々木 朗, 山城 隆, 上岡 寛

    日本矯正歯科学会大会プログラム・抄録集  2019.11  (公社)日本矯正歯科学会

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    Event date: 2019.11

    Language:Japanese  

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  • 重度叢生と歯根短小を伴う著しい骨格性開咬を上下顎外科的矯正治療により改善した症例

    星島 光博, 岡 直毅, 松村 達志, 飯田 征二, 山城 隆, 上岡 寛

    日本顎変形症学会雑誌  2019.5  (NPO)日本顎変形症学会

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    Event date: 2019.5

    Language:Japanese  

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  • SARPE後に上下顎同時移動術を施行し、骨癒合不全を認めた症例

    吉岡 徳枝, 西山 明慶, 星島 光博, 伊原木 聰一郎, 上岡 寛, 佐々木 朗

    日本顎変形症学会雑誌  2019.5  (NPO)日本顎変形症学会

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    Event date: 2019.5

    Language:Japanese  

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  • 多数歯欠損を伴う高齢期の下顎前突症患者に対して外科的矯正治療を行った1例

    河野 加奈, 中村 政裕, 川邉 紀章, 吉岡 徳枝, 西山 明慶, 佐々木 朗, 上岡 寛

    日本顎変形症学会雑誌  2019.5  (NPO)日本顎変形症学会

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    Event date: 2019.5

    Language:Japanese  

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  • 歯科矯正用アンカースクリューを間接的な固定源に用いた上顎前方牽引の有効性

    石原 嘉人, 松田 祐典, 山本 照子, 山城 隆, 上岡 寛

    日本矯正歯科学会大会プログラム・抄録集  2018.10  (公社)日本矯正歯科学会

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    Event date: 2018.10

    Language:Japanese  

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  • 歯科矯正用アンカースクリューを固定源とした大臼歯圧下と犬歯牽引を同時に行う新規治療メカニクスの提案

    白崎 かおり, 石原 嘉人, 古森 紘基, 山城 隆, 上岡 寛

    日本矯正歯科学会大会プログラム・抄録集  2018.10  (公社)日本矯正歯科学会

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  • II級不正咬合症例における歯科矯正用アンカースクリューを併用した下顎切歯圧下の治療結果および安定性

    中村 政裕, Le Thi Thuy Ly, 川邉 紀章, 山城 隆, 上岡 寛

    日本矯正歯科学会大会プログラム・抄録集  2018.10  (公社)日本矯正歯科学会

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  • CCN2とRab14の相互作用が骨・軟骨細胞の基質産生に及ぼす役割

    星島 光博, 服部 高子, 田中 智代, 上岡 寛, 滝川 正春

    日本矯正歯科学会大会プログラム・抄録集  2018.10  (公社)日本矯正歯科学会

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  • Fast Fourier Transform Analysis Showed Morphological Change of Bone Structure and Change of Periodicity of Sclerostin Expression during Orthodontic Tooth Movement.

    Ziyi Wang, Yoshihito Ishihara, Naoya Odagaki, Masahiro Nakamura, Ei Ei Hsu Hlaing, Hiroshi Kamioka

    JOURNAL OF BONE AND MINERAL RESEARCH  2017.12  WILEY

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    Language:English  

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  • Spatio-temporal Regulation of Sclerostin Expression and Dynamics in Alveolar Bone During Orthodontic Tooth Movement.

    Naoya Odagaki, Yoshihito Ishihara, Ziyi Wang, Masahiro Nakamura, Ei Ei Hsu Hlaing, Hiroshi Kamioka

    JOURNAL OF BONE AND MINERAL RESEARCH  2017.12  WILEY

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  • 食餌性状がマウス上顎骨の三次元形態に及ぼす影響

    河野加奈, 谷川千尋, 柳田剛志, 上岡寛, 山城隆

    日本矯正歯科学会大会プログラム・抄録集  2017.10  (公社)日本矯正歯科学会

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  • 軟骨細胞分化に関わる長鎖ノンコーディングRNAの骨形成における役割

    石川 崇典, 村瀬 友里香, 西田 崇, 服部 高子, 大野 充昭, 上岡 寛, 滝川 正春, 久保田 聡

    日本骨代謝学会学術集会プログラム抄録集  2017.7  (一社)日本骨代謝学会

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  • Orthognathic surgery during breast cancer treatment-A case report.

    Tsuyoshi Shimo, Norie Yoshioka, Masahiro Nakamura, Soichiro Ibaragi, Tatsuo Okui, Yuki Kunisada, Masanori Masui, Mayumi Yao, Koji Kishimoto, Shoko Yoshida, Akiyoshi Nishiyama, Hiroshi Kamioka, Akira Sasaki

    International journal of surgery case reports  2017 

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    Event date: 2017

    Language:English  

    INTRODUCTION: In recent years, patients with orthognathic surgery in middle-aged and elderly people have come to be a more frequent occurrence. Breast cancer is the most frequently diagnosed cancer in woman worldwide, and its prevalence rate is steadily increasing. PRESENTATION OF CASE: We report a case of a 47-year-old Japanese woman in whom left-side breast cancer (Stage 1) was unexpectedly found just before orthognathic surgery in April 2012. Breast-conserving surgery was performed (estrogen receptor+, progesterone receptor+, HER2 -, surgical margin+, sentinel lymph node +) that May. From June to August docetaxel (75mg/m2) and cyclophosphamide (600mg/m2) were administrated four times every 21days and thereafter radiotherapy (total 60Gy) was completed. The cancer surgeon declared the prognosis good and the patient had a strong desire to undergo orthognathic surgery, so in November we performed a bimaxillary osteotomy, and administration of tamoxifen began 6 weeks after the osteotomy. DISCUSSION: There are breast cancer cases in which the prognosis is sufficiently good for a planned orthognathic surgery to proceed. Good communication among surgeons and the patient is important. CONCLUSION: We experienced a case in which breast cancer was found just before the orthognathic surgery; we performed a bimaxillary osteotomy, including follow-up tamoxifen administration, during breast cancer treatment.

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  • 馬蹄形骨切り併用Le Fort I型骨切り術における工夫と術後安定性について

    吉岡 徳枝, 西山 明慶, 伊原木 聰一郎, 銅前 昇平, 岸本 晃治, 志茂 剛, 上岡 寛, 佐々木 朗

    日本顎変形症学会雑誌  2016.5  (NPO)日本顎変形症学会

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  • Investigation on long non‐coding RNAs that are associated with chondrocytic phenotype

    ISHIKAWA Takanori, MURASE Yurika, MURASE Yurika, NISHIDA Takashi, HATTORI Takako, TAKIGAWA Masaharu, KAMIOKA Hiroshi, KUBOTA Satoshi, KUBOTA Satoshi

    日本RNA学会年会要旨集  2016 

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    Event date: 2016

    Language:English  

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  • 口蓋裂発生におけるRunx/Cbfbシグナリングの関与

    伊藤 慎将, 三原 聖美, 黒坂 寛, 柳田 剛志, 河野 加奈, 上岡 寛, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集  2014.10  (公社)日本矯正歯科学会

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  • 当科における馬蹄形骨切り併用Le Fort I型骨切り術施行症例の臨床検討

    吉岡 徳枝, 西山 明慶, 伊原木 聰一郎, 福島 宏明, 中村 政裕, 志茂 剛, 上岡 寛, 佐々木 朗

    日本顎変形症学会雑誌  2014.5  (NPO)日本顎変形症学会

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  • 重度の上顎骨狭窄および口蓋瘻孔を伴う両側性唇顎口蓋裂患者の治療例

    福島 宏明, 上岡 寛, 黒坂 寛, 森谷 徳文, 飯田 征二, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集  2013.10  (公社)日本矯正歯科学会

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    Event date: 2013.10

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  • 物理刺激に対する筋骨格系細胞の形態的および機能的応答解析

    佐藤正明, 安達泰治, 上岡寛, 神崎展

    運動器リハビリテーション  2013.6.6 

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    Event date: 2013.6.6

    Language:Japanese  

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  • 包括的な治療計画のもと上下顎骨切り術を行った著しいガミースマイルを伴う下顎後退位症例

    中村 政裕, 本城 正, 片岡 伴記, 上岡 寛, 吉岡 徳枝, 奥井 達雄, 西山 明慶, 佐々木 朗, 山城 隆

    日本顎変形症学会雑誌  2012.5  (NPO)日本顎変形症学会

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    Event date: 2012.5

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  • 骨細管内の間質液流れによる骨細胞突起変形シミュレーション

    亀尾佳貴, 山本隆太, 大多尾義弘, 石原正行, 上岡寛, 安達泰治

    日本機械学会2012 年度年次大会(2012.9.10-12.金沢)  2012 

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    Event date: 2012

    Language:Japanese  

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  • 生体ライブイメージングを用いた骨組織中における自律性細胞内カルシウムオシレーションの検討

    石原 嘉人, 菅原 康代, 上岡 寛, 川邉 紀章, 黒坂 寛, 成瀬 恵治, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集  2011.10  (公社)日本矯正歯科学会

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    Language:Japanese  

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  • 上顎および下顎枝垂直骨切り術により顎運動の改善が見られた顎関節症を併発する骨格性下顎前突症例

    菅原康代, 上岡寛, 山本照子, 山城隆

    日本顎変形症学会雑誌  2011 

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  • 骨細管イメージベースモデルを用いた間質液流れシミュレーション

    亀尾佳貴, 安達泰治, 上岡 寛

    第38 回日本臨床バイオメカニクス学会(2011.11.18-19 神戸)  2011 

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    Language:Japanese  

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  • Microscale Flow Analysis in Bone \nCanaliculi Using High-resolution Image-based Models

    Taiji Adachi, Yoshitaka Kameo, Jenneke Klein-Nulend, Hiroshi Kamioka

    Workshop: Microscale Modeling in Biomechanics and Mechanobiology(2011.5.30-6.1 Ericeira, Prtugal)(Invited Talk)  2011 

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    Event date: 2011

    Language:English  

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  • Microscale Fluid-flow Analysis in Osteocyte Canaliculi Using High-resolution \nImage-based Models.

    Hiroshi Kamioka, Yoshitaka Kameo, Yuichi Imai, Astrid D. Bakker, Rommel G Bacabac, Takashi Yamashiro, Taiji Adachi, Jenneke Klein-Nulend

    American Society of Bone and Mineral Research(2011.9.16-21 San Diego, USA).  2011 

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  • 三次元再構築された骨細管モデル内における流れ解析の試み

    上岡 寛, 亀尾佳貴, 今井裕一, 安達泰治, 山城 隆

    第31 回日本骨形態計測学会(2011.5.20-22 岐阜)  2011 

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    Language:Japanese  

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  • 高詳細骨細管モデルを用いた流れ解析の試み

    上岡 寛, 今井裕一, 亀尾佳貴, 安達泰治, 山城 隆

    第70 回日本矯正歯科学会大会 & 第4 回国際会議(2011.10.17-20 名古屋).  2011 

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    Language:Japanese  

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  • OCFC(Okayama craniofacial center)の紹介 小児頭蓋顔面形成センターの設立

    本城 正, 黒坂 寛, 上岡 寛, 伊達 勲, 木股 敬裕, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集  2010.9  (公社)日本矯正歯科学会

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    Event date: 2010.9

    Language:Japanese  

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  • 0335 力学刺激に対する単離骨系細胞間の情報伝達特性に関する考察(GS4:骨細胞)

    青沼 有紀, 平 圭佑, 安達 泰治, 北條 正樹, 上岡 寛

    バイオエンジニアリング講演会講演論文集  2010.1 

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    Language:Japanese  

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  • Cell-cell communication in living bone and its regulation factor

    Y. Ishihara, H. Kamioka, T. Takano-Yamamoto, T. Yamashiro

    JOURNAL OF BONE AND MINERAL RESEARCH  2007.9  AMER SOC BONE & MINERAL RES

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    Event date: 2007.9

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  • The elastic modulus of osteoblasts and osteocytes

    Y. Sugawara, H. Kamioka, R. Ando, Y. Ishihara, S. A. Murshid, K. Hashimoto, N. Kataoka, K. Tsujioka, F. Kajiya, T. Yamashiro, T. Takano-Yamamoto

    JOURNAL OF BONE AND MINERAL RESEARCH  2007.9  AMER SOC BONE & MINERAL RES

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    Event date: 2007.9

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  • Actin and microtubule cytoskeletons of the processes of 3D-cultured MC3T3-E1 cells and osteocytes (vol 25, pg 151, 2007)

    Sakhr A. Murshid, Hiroshi Kamioka, Yoshihito Ishihara, Ryoko Ando, Yasuyo Sugawara, Teruko Takano-Yamamoto

    JOURNAL OF BONE AND MINERAL METABOLISM  2007.7  SPRINGER TOKYO

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    Event date: 2007.7

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  • 説明系OSCE開始直後の評価の信頼性

    宮脇正一, 出口徹, 村上薫, 本城正, 福永智宏, 上岡寛, 吉田登志子, 山本照子

    日本歯科医学教育学会雑誌  2007 

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  • 咬筋肥大症の患者に対して咬筋切除術と矯正治療を行った2症例.

    橋本隆志, 黒田晋吾, 上岡寛, 宮脇正一, 三島克章, 菅原利夫, 山本照子

    岡山歯学会雑誌  2006.6.30  岡山歯学会

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    Language:Japanese  

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  • Mechanosensitivity in primary osteocytes

    Kamioka H, Sugawara, Y, Murshid SA Ishihara Y, Honjo T, Takano-Yamamoto T

    Japanese Society for Medical and Biological Engineering,  2006 

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  • 流体剪断力を負荷した培養骨細胞は骨芽細胞,ライニング細胞に比べ細胞内カルシウム応答を示す細胞が少ない

    上岡 寛, 菅原 康代, 本城 正, 黒坂 寛, ムルシド・サクル, 山本 照子

    日本矯正歯科学会大会プログラム・抄録集  2004.11  (公社)日本矯正歯科学会

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  • Actin-binding proteins in osteocytes and osteoblasts.

    H Kamioka, Y Sugawara, T Honjo, T Yamashiro, T Takano-Yamamoto

    JOURNAL OF DENTAL RESEARCH  2003.12  INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R

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  • 骨格性下顎前突症にRigid External Distraction systemを適用した仮骨延長の一症例

    大矢 伸治, 山下 和夫, 上岡 寛, 山本 照子, 香川 智正, 三島 克章, 菅原 利夫

    日本顎変形症学会雑誌  2001.8  (NPO)日本顎変形症学会

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  • 非焦点レーザー顕微鏡と透過レーザー微分干渉顕微鏡を併用した骨細胞細胞突起の3次元的形態観察

    上岡寛, 本城正, 福永智広, 山本照子

    日本矯正歯科学会大会プログラム・抄録集  2000.10.25 

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  • 多数歯先天欠如,劣成長を伴う上顎部にREDシステムを適用した仮骨延長症例の形態的,機能的評価

    大矢 伸治, 上岡 寛, 藤木 辰哉, 山城 隆, 山本 照子, 三島 克章, 菅原 利夫

    日本矯正歯科学会大会プログラム・抄録集  2000.10  (公社)日本矯正歯科学会

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  • 多数歯先天欠如,劣成長を伴う上顎部にREDシステムを適用した仮骨延長の1症例 術前・術後の形態的・機能的評価

    大矢 伸治, 上岡 寛, 藤木 辰哉, 山城 隆, 山本 照子, 香川 智正, 三島 克章, 菅原 利夫

    日本顎変形症学会雑誌  2000.8  (NPO)日本顎変形症学会

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  • ISOLATION OF CDNA ENCORDING SPECIFIC PROTEIN IN BONE FORMING CELL

    M OHTA, K TEZUKA, T MATSUO, H KAMIOKA, Y HAKETA, K TANAKA, K SUZUKI, M KUMEGAWA

    JOURNAL OF BONE AND MINERAL RESEARCH  1992.8  BLACKWELL SCIENCE INC

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    Event date: 1992.8

    Language:English  

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  • 内分泌撹乱物質AhRリガンドはCyp1a1シグナルを介して破骨細胞分化および骨代謝を制御する

    吉川友理, 井澤 俊, 上岡 寛

    第63回歯科基礎医学会学術大会  2021.10.9 

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    Country:Japan  

  • 核内受容体AhRはRANK/c-Fosシグナル伝達経路を介して破骨細胞の分化を制御する

    井澤 俊, 吉川 友理, 石丸直澄, 上岡 寛

    第40回 日本骨形態計測学会  2020.6.19  日本骨形態計測学会

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 下顔面高が顔貌の審美性へ与える影響について

    第76回日本矯正歯科学会大会  2017 

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  • 矯正的歯の移動によるsclerostin発現の変化は歯槽骨改造を調節する

    第59回歯科基礎医学会学術大会  2017 

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  • Detection of the bone structure change and periodic osteocytesʼ expression

    第59回歯科基礎医学会学術大会  2017 

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  • 骨細胞ネットワーク形成に与えるコラーゲン線維構築の関与

    第59回歯科基礎医学会学術大会  2017 

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  • Sclerostinは矯正的歯の移動時の圧迫側および牽引側で生じる対照的骨代謝調節の制御因子である

    第76回日本矯正歯科学会学術大会  2017 

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  • ギャップ結合とConnexin43ヘミチャネルは骨細胞へのメカニカルストレスに由来したカルシウム応答を制御する

    第76回日本矯正歯科学会学術大会  2017 

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  • 食餌性状がマウス上顎骨の三次元形態に及ぼす影

    第76回日本矯正歯科学会学術大会  2017 

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  • 骨細胞・骨細管とコラーゲンの微細構築

    歯科基礎医学会  2017 

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  • 軟骨細胞分化に関わる長鎖非コードRNAの骨形成における役割

    第35回日本骨代謝学会  2017 

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  • 骨組織中骨細胞へ負荷されたメカニカルストレス由来のカルシウム応答はコネキシン43ヘミチャネルによって制御される

    第35回日本骨代謝学会学術集会  2017 

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  • 歯科矯正用アンカースクリューを用いて治療した開咬患者の下顎運動評価

    第76回日本矯正歯科学会学術大会  2017 

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  • III級不正咬合症例における歯科矯正用アンカースクリュー使用群とIII級ゴム使用群の治療結果の比較検討

    第76回日本矯正歯科学会学術大会  2017 

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  • Evidence for the bone structure change andosteocytes' biorhythm during orthodontic toothmovement

    第76回日本矯正歯科学会学術大会  2017 

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  • 著しい骨格性下顎前突および多数歯欠如を伴う色素失調症患者に対し、外科的矯正治療を行った一例

    第76回日本矯正歯科学会学術大会  2017 

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  • FIB-SEMで探る骨形成 ーコラーゲン細線維の集束化と骨細胞ネットワーク形成の関連についてー

    日本臨床バイオエンジニアリング講演会  2017 

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  • 歯科矯正用アンカースクリューとIII級ゴムを併用し下顎骨の回転を防いだ骨格性下顎前突カムフラージュ症例

    第76回日本矯正歯科学会学術大会  2017 

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  • 機能的矯正装置の使用により良好な成長変化を示したGoldenhar症候群の一治験例

    第76回日本矯正歯科学会学術大会  2017 

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  • 歯科矯正用アンカースクリューを用いて治療した開咬症例の長期保定

    第76回日本矯正歯科学会学術大会  2017 

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  • エーラス・ダンロス症候群を伴う骨格性の上顎前突、側方偏位症例に対するカムフラージュ治療

    第76回日本矯正歯科学会大会  2017 

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  • 骨細胞の成熟過程におけるPerlecan/HSPG2の発現解析

    第76回日本矯正歯科学会学術大会  2017 

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  • CCN2とRab14の相互作用が骨・軟骨細胞の小胞輸送に及ぼす役割

    2017年度生命科学系学会合同年次大会 第40回日本分子生物学会 第90回日本生化学会大会  2017 

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  • A case of BCLP treated with MASDO

    The 41th Annual Meeting of Japanese Cleft Palate Association  2017 

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  • A novel three-dimensional evaluation of the skeletal changes in the mandible associated with soft-diet feeding

    The 60th Annual Meeting of the CHU-SHIKOKU Orthodontic Society  2017 

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  • A case of mandibular prognathism with severe maxillomandibular crowding and narrow maxillary arch

    The 60th Annual Meeting of the CHU-SHIKOKU Orthodontic Society  2017 

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  • BCG TokyoRD16領域に存在おするJTY_3475cはJTY_3476の遺伝子発現を負に制御する

    第90回日本細菌学会総会  2017 

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  • 生物学・材料学の複合的アプローチによる頭蓋骨初期石灰化機構の理解

    第16回日本再生医療学会総会  2017 

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  • マウス頭蓋骨石灰化開始点の同定及び無機質の定性・定量解析

    第5回日本バイオマテリアル学会中四国ブロックシンポジウム  2017 

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  • Sclerostin modulates alveolar bone turnover during orthodontic tooth movement

    The 59th Annual Meeting of Japanese Association for Oral Biology  2017 

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  • 骨組織におけるコラーゲン細線維の三次元形態計測

    日本顕微鏡学会第73回学術大会  2017 

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  • 上顎骨前方部骨延長術を行った両側性口唇口蓋裂の一症例

    第41回日本口蓋裂学会総会・学術集会  2017 

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  • 鼻中隔および舌に奇形腫を伴った左側唇顎口蓋裂患者の 1 例

    第41回日本口蓋裂学会総会・学術集会  2017 

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  • Vertical facial type variations influence the aesthetic preference of anteroposterior lip positions

    2017 Annual Session of American Association of Orthodontists  2017 

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  • The osteocyte network formation is influenced by the collagen fibril alignment

    The 59th Annual Meeting of Japanese Association for Oral Biology  2017 

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  • Comprehensive orthodontic treatment of Leprechaunism syndrome with anterior open bite and severe crowding: A case report

    93rd European Orthodontic Society Congress  2017 

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  • 上下顎重度叢生、上顎大臼歯間幅径狭窄を伴う骨格性下顎前突症例に対する矯正治療の一例

    第60回中・四国矯正歯科学会大会  2017 

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  • 歯科矯正用アンカースクリューを用いて多数埋伏歯の同時牽引を行った鎖骨頭蓋異骨症患者の1例

    第60回中・四国矯正歯科学会大会  2017 

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  • 睡眠時無呼吸症候群を伴う患者の下顎骨過小を仮骨延長術により改善した症例

    第60回中・四国矯正歯科学会大会  2017 

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  • III級不正咬合症例における歯科矯正用アンカースクリュー使用群とIII級ゴム使用群の治療結果の比較検討

    9th World Implant Orthodontic Conference  2017 

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  • Orthodontic tooth movement contrastingly regulates SOST/Sclerostin expression in alveolar bone

    第35回日本骨代謝学会学術集会  2017 

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  • Orthognathic surgery for a case demonstrating a remarkable displacement of the maxilla and mandible: Achieving a good functional balance and a satisfactory aesthetic appearance

    8th Mandalay Dental Conference  2017 

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  • Goldenhar syndrome; Non-surgical orthodontic treatment approach by modification of dentofacial growth

    8th Mandalay Dental Conference  2017 

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  • 食餌性状がマウス下顎骨の三次元的形態変化に及ぼす影響

    第60回中・四国矯正歯科学会大会  2017 

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  • 直交配置型FIB-SEMでみる、骨細胞ネットワーク形成とコラーゲン細線維構築の関与

    第37回骨形態計測学会  2017 

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  • 開咬患者における歯性および骨格性に上顎臼歯部圧下を行った場合の咀嚼運動評価

    第27回日本顎変形症学会総会・学術大会  2017 

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  • Vertical facial type variations influence the aesthetic preference of anteroposterior lip positions

    2017 Annual Session of American Association of Orthodontists  2017 

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  • A case of traction of many impacted teeth in a patient of cleidocranial dysostosis using orthodontic anchor screws

    The 60th Annual Meeting of the CHU-SHIKOKU Orthodontic Society  2017 

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  • Mandibular distruction osteogenesis for micrognathia in a patient with sleep apnea syndrome

    The 60th Annual Meeting of the CHU-SHIKOKU Orthodontic Society  2017 

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  • The evaluation of masticatory movement between dental and skeletal impaction of maxillary molars

    The 27th Annual Meeting of the society for Jaw Deformities  2017 

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  • The relationship between osteocyte network formation and thick collagen bundle formation during bone modeling

    The 75th Annual Meeting of the Japanese Orthodontic Society  2016 

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  • Novel intracellular function of CCN2 -Role of interaction between CCN2 and Rab14 in vesicle trafficking in chondrocytes-

    ASBMR 2016 Annual Meeting  2016 

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  • Analysis of intracellular Ca2+ mobilization by 3D time-lapse imaging in bone

    ASBMR 2016 Annual Meeting  2016 

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  • Osteocyte Network Formation is Influenced by the Thick Collagen Bundle Formation during Bone Modeling

    ASBMR 2016 Annual Meeting  2016 

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  • 骨細胞ネットワーク形成に与えるコラーゲン線維集束化の関与

    第58回歯科基礎医学会学術大会  2016 

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  • 骨組織の3次元タイムラプスイメージングによる流体せん断応力に対する細胞内カルシウム応答の解析

    第75回日本矯正歯科学会大会  2016 

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  • モデリング期骨組織における骨細胞ネットワーク形成とコラーゲン線維集束化の関与

    第75回日本矯正歯科学会大会  2016 

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  • 歯科矯正学分野「歯の移動の臨床手技実習」における60分授業・4学期制への対応―学生アンケート結果からみた現状と今後―

    第37回岡山歯学会総会・学術集会  2016 

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  • CCN2とRab14の相互作用が軟骨細胞の小胞輸送に及ぼす役割

    第75回日本矯正歯科学会大会  2016 

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  • 食餌性状がマウス下顎骨の三次元形態に及ぼす影響

    第75回日本矯正歯科学会大会  2016 

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  • モデリング期骨組織における骨細胞ネットワーク形成とコラーゲン線維集束化の関与

    第75回日本矯正歯科学会大会  2016 

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  • 正貌の非対称性に関する審美的認識と外科的治療の必要性

    第75回日本矯正歯科学会大会  2016 

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  • 矯正歯科治療前および治療済の患者における口腔関連Quality of Lifeと各種歯科矯正学的指標の関連性

    第75回日本矯正歯科学会大会  2016 

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  • 前歯部開咬および重度叢生を伴う妖精症の一例

    第75回日本矯正歯科学会大会  2016 

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  • 下歯槽神経切断によるラット下顎切歯の形態形成と歯原性間葉系幹細胞の減少

    第75回日本矯正歯科学会大会  2016 

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  • 頭蓋骨初期発生における生物学的・材料学的解析による骨石灰化メカニズムの理解

    第75回日本矯正歯科学会大会  2016 

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  • 口唇裂・口蓋裂患者に対する包括的アプローチについて

    第40回日本遺伝カウンセリング学会学術集会  2016 

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  • A biology-material science-based approach for understanding intramembranous bone mineralization mechanism in early developmental stages of mouse calvaria

    The 75th Annual Meeting of the Japanese Orthodontic Society  2016 

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  • Comparison of Oral Health-related Quality of Life with orthodontic indices in patients before/after orthodontic treatment

    The 75th Annual Meeting of the Japanese Orthodontic Society  2016 

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  • A case of Leprechaunism syndrome with anterior open bite and severe crowding

    The 75th Annual Meeting of the Japanese Orthodontic Society  2016 

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  • Comparative evaluation of treatment outcomes between temporary anchorage devices and Class III elastics in Class III malocclusion.

    92nd European Orthodontic Society Congress  2016 

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  • 歯科矯正用アンカースクリューを用いた骨格性Ⅲ級不正咬合症例の矯正歯科治療の長期安定性 ―顎顔面形態と顎口腔機能との関係―

    第32回東北矯正歯科学会大会  2016 

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  • モデリング期骨組織におけるコラーゲン線維の三次元的形態計測

    日本顕微鏡学会第72回学術講演会  2016 

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  • 馬蹄形骨切り併用Le Fort I 型骨切り術における工夫と術後安定性について

    第26回特定非営利活動法人日本顎変形症学会総会  2016 

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  • モデリング期骨組織におけるコラーゲン細線維の三次元的形態計測

    第36回骨形態計測学会  2016 

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  • 馬蹄形骨切り併用Le Fort I型骨切り術とIVROによる外科的矯正治療を行った偏位を伴う骨格性下顎前突症例

    第26回特定非営利活動法人日本顎変形症学会総会  2016 

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  • 頭蓋骨初期発生における生物学的・材料学的解析による骨石灰化メカニズムの理解

    第37回日本炎症再生医学会  2016 

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  • 片側馬蹄形骨切り併用Le FortⅠ型骨切り術と下顎枝垂直骨切り術を用いて外科的矯正治療を行った偏位を伴う骨格性下顎前突症例

    第59回中・四国矯正歯科学会大会  2016 

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  • 骨格性下顎前突患者におけるIVROとSSRO術後の顎運動機能比較

    第59回中・四国矯正歯科学会大会  2016 

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  • 開咬治療の術前術後の下顎運動の比較:歯科矯正用アンカースクリュー使用群と外科的矯正治療群の比較

    第26回特定非営利活動法人日本顎変形症学会総会  2016 

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  • 骨格性下顎前突患者における顎運動機能の評価方法の検討と、IVROとSSRO術後の運動機能比較

    第26回特定非営利活動法人日本顎変形症学会総会  2016 

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  • 歯科矯正用アンカースクリュー破折の原因に関する報告

    第59回中・四国矯正歯科学会大会  2016 

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  • モデリング期での骨細胞ネットワーク形成に与えるコラーゲン線維集束化の関与

    第34回日本骨代謝学会学術集会  2016 

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  • モデリング期骨組織におけるコラーゲン線維の三次元的形態計測

    第34回日本骨代謝学会学術集会  2016 

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  • Analysis of Intracellular Ca2+ Mobilization of Osteoid-Osteocytes and Mature Osteocytes by 3D Time-lapse Imaging in Bone

    ESA/SRB/ANZBMS Joint meeting 2016  2016 

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  • Application of orthodontic treatment for the periodontal and restorative goals in Adults

    7th Mandalay Dental Conference & Mid-year Meeting of Myanmar Dental Association  2016 

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  • Alternation in the gap-junctional intercellular communication capacity during the maturation of osteocytes in the embryonic chick calvaria

    第34回日本骨代謝学会学術集会  2016 

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  • 骨組織の3次元タイムラプスイメージングによる細胞内カルシウム応答の解析

    第34回日本骨代謝学会学術集会  2016 

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  • A report on fracture of orthodontic temporary anchorage device

    The 59th Annual Meeting of the CHU-SHIKOKU Orthodontic Society  2016 

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  • Postoperative stability and some tips of Le Fort I with horseshoe osteotomy

    2016 

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  • Comparative evaluation of treatment outcomes between temporary anchorage devices and Class III elastics in Class III malocclusion.

    92nd European Orthodontic Society Congress  2016 

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  • Osteocyte Network Formation is Influenced by the Thick Collagen Bundle Formation during Bone Modeling

    ASBMR 2016 Annual Meeting  2016 

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  • Analysis of Intracellular Ca2+ Mobilization of Osteoid-Osteocytes and Mature Osteocytes by 3D Time-lapse Imaging in Bone

    ESA/SRB/ANZBMS Joint meeting 2016  2016 

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  • Application of orthodontic treatment for the periodontal and restorative goals in Adults

    7th Mandalay Dental Conference & Mid-year Meeting of Myanmar Dental Association  2016 

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  • Alternation in the gap-junctional intercellular communication capacity during the maturation of osteocytes in the embryonic chick calvaria

    2016 

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  • The influence of facial asymmetry on esthetic preference and perceived need for orthognathic surgery

    The 75th Annual Meeting of the Japanese Orthodontic Society  2016 

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  • Novel intracellular function of CCN2 -Role of interaction between CCN2 and Rab14 in vesicle trafficking in chondrocytes-

    ASBMR 2016 Annual Meeting  2016 

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  • Analysis of intracellular Ca2+ mobilization by 3D time-lapse imaging in bone

    ASBMR 2016 Annual Meeting  2016 

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  • Reduced mesenchymal stem cell and incisal malformation after inferior alveolar nerve neurectomy in rats

    The 75th Annual Meeting of the Japanese Orthodontic Society  2016 

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  • Roles of interaction between CCN2 and Rab14 in vesicle trafficking in chondrocytes

    The 75th Annual Meeting of the Japanese Orthodontic Society  2016 

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  • Three-dimensional evaluation that diet property influences the characteristics of mandibular morphology

    The 75th Annual Meeting of the Japanese Orthodontic Society  2016 

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  • III級不正咬合症例における歯科矯正用アンカースクリューの使用基準

    第58回中・四国矯正歯科学会  2015 

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  • Hardness of food influences the characteristics of masseter muscle subsequently to myh genes expression

    Congress of european orthodontic society,2015  2015 

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  • 巨核球および血小板に存在するCCNファミリータンパク質の存在様態とその由来

    第33回日本骨代謝学会学術集会  2015 

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  • 著しい上顎骨狭窄を呈する軟口蓋裂成人症例に対し、変則的SARPEにより外科的矯正治療を行った一例

    第58回中・四国矯正歯科学会  2015 

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  • Effects of asymmetric canine substitution on the perception of smile attractiveness

    European Orthodontic Society  2015 

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  • 血小板におけるCCNファミリータンパク質の同定とその役割

    第56回日本生化学会 中国・四国支部例会  2015 

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  • 変則的SARPEにより外科的矯正治療を行った,著しい上顎骨の狭窄を呈する軟口蓋裂成人患者

    第39回日本口蓋裂学会総会・学術集会  2015 

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  • 口蓋形成におけるRunx/Cbfbシグナリングの関与

    第39回日本口蓋裂学会総会・学術集会  2015 

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  • Modification of transverse maxillary distruction osteogenesis for a cleft palate patients

    The 39th Annual Meeting of Japanese Cleft Palate Association  2015 

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  • Runx/Cbfb signaling is involeved in the cleft palate formation

    The 39th Annual Meeting of Japanese Cleft Palate Association  2015 

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  • Comprehensive analysis of the molecule mechanism in Primary Failure of Eruption (PFE)

    The 58th Annual Meeting of the CHU-SHIKOKU Orthodontic Society  2015 

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  • Hardness of diet changes a mandibular morphology form due to increased or decreased Myh gene expressions in a masseter muscle

    The 58th Annual Meeting of the CHU-SHIKOKU Orthodontic Society  2015 

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  • Hardness of food influences the characteristics of masseter muscle subsequently to myh genes expression

    Congress of european orthodontic society,2015  2015 

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  • Effects of asymmetric canine substitution on the perception of smile attractiveness

    European Orthodontic Society  2015 

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  • SEM連続断面観察による生物組織三次元再構築法研究部会「未脱灰骨組織超微観察の新たな目 - FIB-SEM」

    日本顕微鏡学会  2015 

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  • CCN2とRab14の相互作用が軟骨細胞の小胞輸送に及ぼす役割

    第28回 日本軟骨代謝学会  2015 

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  • Hardness of food influences the characteristics of masseter muscle subsequently to Myh genes expression

    The 74th Annual Meeting of the Japanese Orthodontic Society  2015 

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  • Role of interaction between CCN2 and Rab14 in vesicle trafficking in chondrocytes novel intracellular function of CCN2.

    2015 

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  • 重度下顎骨後退位を伴った左側唇顎口蓋裂症例

    第39回日本口蓋裂学会総会・学術集会  2015 

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  • 岡山大学病院矯正歯科における口唇裂・口蓋裂患者の実態調査

    第39回日本口蓋裂学会総会・学術集会  2015 

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  • 下顎の偏位と上下顎歯列弓形態異常を伴う片側性唇顎口蓋裂症例に対する非外科的矯正歯科治療の長期予後

    第39回日本口蓋裂学会総会・学術集会  2015 

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  • Collagen production of osteoblasts revealed by ultra-high voltage electron microscopy

    ASBMR 2015 Annual Meeting  2015 

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  • A survey of patient's attitudes and over all satisfaction for orthodontic treatment at Okayama University Hospital Orthodontic Clinic.

    2015 

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  • A case of adult cleft palate patient with a severe narrowed maxillary arch treated with uneven SARPE

    The 58th Annual Meeting of the CHU-SHIKOKU Orthodontic Society  2015 

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  • CCN2とRab14の相互作用が軟骨細胞の小胞輸送に及ぼす役割 ~軟骨分化促進因子CCN2の新たな細胞内機能~

    第57回歯科基礎医学会  2015 

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  • CCN2の新たな細胞内機能:CCN2とRab14の相互作用が軟骨細胞の小胞輸送に及ぼす役割

    第7回 日本CCNファミリー研究会  2015 

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  • 粉末餌で飼育されたマウスでは、咬筋でのMyh遺伝子の発現様態と下顎骨形態に変化を生じる

    第58回中・四国矯正歯科学会  2015 

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  • Collagen production of osteoblasts revealed by ultra-high voltage electron microscopy

    ASBMR 2015 Annual Meeting  2015 

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  • 岡山大学病院矯正歯科を受診している患者の矯正治療に対する意識と満足度の調査

    第36回岡山歯学会総会・学術集会  2015 

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  • 骨細胞の組織内蛍光イメージングと形態計測

    日本機械学会2015年度年次大会  2015 

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  • 直交配置型FIB-SEMを用いた骨組織の三次元的解析

    第57回歯科基礎医学会学術大会  2015 

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  • Hardness of food influences the characteristics of masseter muscle subsequently to Myh genes expression

    第74回日本矯正歯科学会大会  2015 

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  • Runx/Cbfbシグナリングはアンドロゲン代謝を介して唾液腺の雌雄二形性発現を制御する

    第74回日本矯正歯科学会大会  2015 

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  • エーラス・ダンロス症候群を伴う骨格性の上顎前突、上下顎骨側方偏位症例に対するカムフラージュ治療

    第74回日本矯正歯科学会大会  2015 

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  • Role of interaction between CCN2 and Rab14 in vesicle trafficking in chondrocytes novel intracellular function of CCN2.

    第8回 国際CCNファミリー研究会  2015 

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  • 非症候群性原発性萌出不全(PFE)患者歯牙歯根膜における遺伝子発現様態の網羅的解析

    第58回中・四国矯正歯科学会  2015 

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  • 直交配置型FIB-SEMによる骨組織の観察

    日本顕微鏡学会関西支部  2014 

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  • 「著しい上下顎骨の偏位を伴う症例への外科的矯正治療」

    第25回日本歯科審美学会学術大会教育講演  2014 

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  • 直交配置型FIB-SEMによる骨の3次元的観察

    第56回歯科基礎医学会 サテライトシンポジウム  2014 

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  • SEM連続断面観察による3次元微細構造解析法 「直交配置型FIB-SEMを用いた骨組織の観察」

    第119回日本解剖学会シンポジウム  2014 

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  • 画像 イメージングと骨形態計測の接点

    第34回日本骨形態計測学会医⻭工連携シンポジウム  2014 

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  • 「骨組織の動態を探る蛍光イメージング」

    日本生化学会中国・四国支部例会シンポジウム  2014 

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  • 骨細胞のイメージングによる機能解析

    国立長寿医療研究センター運動器疾患研究部主催セミナー  2011 

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  • 骨細管内体液の流れ解析の試み

    第29回日本骨代謝学会  2011 

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  • Microscale flow analysis in bone canaliculi using high-resolution image-based models

    Workshop: Microscale Modeling in Biomechanics and Mechanobiology  2011 

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  • 骨細胞(オステオサイト)に注目した骨アパタイト配向化制御機構の材料学的手法による解明

    日本金属学会・日本鉄鋼協会関西支部 材料物性工学談話会  2011 

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  • Microscale flow analysis in bone canaliculi using high-resolution image-based models

    Workshop: Microscale Modeling in Biomechanics and Mechanobiology  2011 

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  • 鍍銀染色された骨細胞の超高圧電子顕微鏡による観察

    第68回日本矯正歯科学会  2009 

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  • 骨細胞の形態と機能

    機能再生・再建科学専攻系ミニシンポジウム <基礎研究と臨床研究のインターフェイス>  2009 

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  • ニワトリとマウスの骨細胞ネットワークの3次元形態計測

    第29回日本骨形態計測学会  2009 

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  • 骨細胞のバイオイメージング

    第29回日本骨形態計測学会  2009 

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  • 単離骨系細胞におけるカルシウム応答伝搬の非対称性

    日本機械学会2009年度年次大会  2009 

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  • Observation of Calcium Signaling Response of Osteocytes to Bone Matrix Deformation

    Third Switzerland-Japan workshop on Biomechanics 2009  2009 

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  • 外傷性咬合ならびに歯周疾患を伴う成人下顎前突症例

    第51回中・四国矯正歯科学会大会  2009 

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  • 骨格性II級開咬症例に対する非抜歯2段階治療

    第68回日本矯正歯科学会  2009 

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  • SSEA-4を用いたヒト歯根膜幹細胞の同定

    第68回日本矯正歯科学会  2009 

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  • 実験的歯の移動時におけるc-fosの発現とグリア細胞の動態との関連性について

    第68回日本矯正歯科学会  2009 

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  • 骨小腔ー骨細管ネットワークを考慮した骨梁の力学解析

    第20回 バイオエンジニアリング講演会  2008 

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  • Bone as mechanosesory organ

    Radboud University Nijmegen Medical Centre, Dept. of Orthodontics and Oral Biology (Prof. Kuijpers-Jagtman)  2008 

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  • メカニカルセンサーとしての骨組織

    東京工業大学統合研究院 大学院生命理工学研究室 生命情報専攻主催セミナー (半田宏教授)  2008 

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  • 骨細胞形態のナノレベル解析と機械的特性について

    せとうちBME  2008 

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  • 単離骨系細胞間のカルシウム応答伝播特性

    機械学会関西支部卒業研究発表講演会  2008 

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  • Osteocyte in Bone Revealed by Electron Tomography

    Ninth International Bone Fluid Flow Workshop  2008 

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  • 骨細胞の形態・機能解析から探るメカニカルセンサーとしての骨組織

    東京医科歯科大学 大学院 医歯学総合研究科 分子細胞機能学主催セミナー(森田育男教授)  2008 

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  • 単離骨細胞における局所的な力学刺激応答特性

    第31回日本バイオレオロジー学会  2008 

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  • 骨細胞を中心とした骨微細構造のナノレベル解析

    第28回骨形態計測学会  2008 

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  • ミニスクリューを不動固定に用いて下顎第三大臼歯を近心移動した開咬症例

    第51回中・四国矯正歯科学会大会  2008 

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  • 過去10年間に岡山大学医学部・歯学部附属病院矯正歯科に来院した矯正患者の実態調査

    第51回中・四国矯正歯科学会大会  2008 

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  • Botulinum toxin blocks neurotransmitter release from somata of rat neuropathic trigeminal ganglion neurons

    Toxin 2008  2008 

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  • 骨基質の変形に対する骨細胞のカルシウム応答観察

    第26回日本骨代謝学会  2008 

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  • 細胞内カルシウムが生骨組織中における細胞間コミュニケーションの調節へ与える影響

    第67回日本矯正歯科学会  2008 

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  • Cell-cell communication of osteocytes in living bone and its regulation factors

    Anglo-Japanese Symposium:Network for international education and research in advanced dental science  2008 

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  • The three-dimensional morphology of osteocytes network in chick and mouse calvaria

    The 1st International Symposium of Medical and Dental Education in Okayama  2008 

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  • Regulation of cell-cell communication in osteocytes in living bone

    The 1st International Symposium of Medical and Dental Education in Okayama  2008 

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  • 骨芽細胞株MC3T3-E1細胞において流体剪断応力はMAPKを介してCTGFの発現を増大させる

    第65回日本矯正歯科学会  2007 

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  • 骨の機械的刺激への応答能を医工学的に解析するためにー骨細胞の形態・機能の解析ー

    岐阜大学医学部組織・器官形成分野特別講演  2007 

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  • 骨細胞とメカニカルストレスについて

    第2回インターフェイス口腔健康科学国際シンポジウム  2007 

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  • 骨基質内における骨細胞の変形および細胞応答の観察

    第19回バイオエンジニアリング講演会  2007 

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  • マイクロパーティクルを用いた直接変形負荷に対する単離骨細胞のカルシウム応答

    第19回バイオエンジニアリング講演会  2007 

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  • 単離骨細胞の細胞突起/細胞体の力学的刺激に対するカルシウム応答の違い

    第25回日本骨代謝学会  2007 

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  • 骨芽細胞から骨細胞への弾性の変化について

    第25回日本骨代謝学会  2007 

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  • 生骨組織中での細胞間コミュニケーションとその外的調節因子について

    第25回日本骨代謝学会  2007 

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  • 骨芽細胞から骨細胞への弾性の変化について

    第65回日本矯正歯科学会  2007 

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  • 生骨組織中での細胞間コミュニケーションに影響する外的調節因子について

    第65回日本矯正歯科学会  2007 

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  • 骨細胞における機械的刺激への応答能-focal adhesionと細胞弾性率の関与

    第25回日本骨代謝学会  2007 

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  • 超高電圧TEMトモグラフィーを用いた骨細胞周囲の高精細構造・応力解析

    第25回日本骨代謝学会  2007 

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  • Mechanosensitivity of a single osteocyte: Difference in cell process and cell body

    Third Asian Pacific Conference on Biomechanics  2007 

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  • 含歯性嚢胞を伴う多数歯埋伏を有する患者に対する一矯正治療例

    第65回日本矯正歯科学会  2007 

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  • 骨細胞ネットワークによるメカノセンサーシステムの力学応答観察

    第35回FRPシンポジウム  2006 

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  • 骨細胞の形態・メカニカル応答について

    日本生体医工学専門別部会「バイオメカニクス研究会」  2006 

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  • 生骨組織への力学刺激に対する骨細胞カルシウム応答のリアルタイム解析

    富士裾野フォーラム(帝人)  2006 

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  • Three-dimensional quantitative analysis of CGRP during physiological tooth movement

    84th International Association of Dental Research  2006 

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  • Micro-elasticity of osteoblasts and osteocytes

    84th International Association of Dental Research  2006 

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  • Sketetal class III case treated with distal movement of mandibular arch using titanium screw anchorage

    5th Asia Implant Orthodontics Congress  2006 

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  • 素敵な笑顔のための矯正治療

    第27回岡山歯学会総会 市民公開講座  2006 

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  • Osteocyte Biology Using Fluorescent Probes

    21世紀COEプログラム 動的機能機械システムの数理モデルと設計論 京都大学主催セミナー  2006 

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  • Srugical orthodontic treatment for a patient with unilateral cleft lip and temporomandibular disorder

    1st Joint meeting of JOS and KAO  2006 

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  • Histomorphometric evaluation of cortical bone thickness surroudning miniscrew for orthodontic anchorage

    1st Joint meeting of JOS and KAO  2006 

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  • 上顎および下顎枝垂直骨切り術により顎運動の改善が見られた顎関節症を併発する骨格性下顎前突症例

    第65回日本矯正歯科学会  2006 

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  • 小さいお子さんの矯正相談

    邑久郡歯科医師会主催 「矯正歯科相談会」  2006 

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  • Calcium responses of primary chick bone cells to fluid flow.

    84th International Association of Dental Research  2006 

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  • 生理的はの移動時のカルシトニン遺伝子関連ペプチドと破骨細胞および骨芽細胞との3次元組織学的形態計測

    第65回日本矯正歯科学会  2006 

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  • Osteocyte in Bone Revealed by Electron Tomography

    16th International Congress of the IFAA  2006 

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  • Bimaxillary protrusion with masseter hypertrophy treated using titanium screw anchorage and masseter surgical reduction

    5th Asia Implant Orthodontics Congress  2006 

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  • 骨芽細胞から骨細胞への最終分化に伴うアクチン結合蛋白の局在変化について

    第25回岡山歯学会  2005 

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  • 流体剪断応力を負荷した時の、骨細胞および骨芽細胞の細胞内カルシウム応答の違い

    第64回日本矯正歯科学会  2005 

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  • 骨芽細胞から骨細胞への最終分化に伴うアクチン結合蛋白の局在変化について

    第26回岡山歯学会総会  2005 

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  • Quantitative evaluation of cortical bone thickness using CT scanning for implant-orthodontics

    38th Annual Meeting of KAO  2005 

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  • 局所変形負荷に対する単離骨細胞のカルシウム応答

    第16回バイオフロンティア講演会  2005 

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  • 三次元CTを用いたインプラントアンカー植立部位における皮質骨の厚さの定量学的分析

    第64回日本矯正歯科学会  2005 

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  • 力学刺激に対する単離骨細胞の応答観察

    日本機械学会 2005年度年次大会  2005 

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  • 骨細胞の流体剪断応力への細胞内カルシウム応答は骨細胞に比べて低く、その違いは細胞接着部位の形成の違いによってなされている

    第23回日本骨代謝学会  2005 

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  • Comparison of actin cytoskeleton between 2D and 3D cultured MC3T3-E1

    第53回国際歯科研究学会日本部会(JADR)総会・学術大会  2005 

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  • Three-dimensional quantitative analysis of CGRP during physiological tooth movement

    第53回国際歯科研究学会日本部会(JADR)総会・学術大会  2005 

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  • Bone physiome

    第53回国際歯科研究学会日本部会(JADR)総会・学術大会  2005 

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  • 咬筋肥大症の患者に対して咬筋切除術と矯正治療を行った2症例

    第26回岡山歯学会総会  2005 

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  • 骨細胞の3次元的形態計測

    第26回岡山歯学会総会  2005 

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  • αVβ3 インテグリンは骨細胞において伸展刺激によるカルシウム流入系を特異的に刺激しメカニカルストレス受容に関与する

    第23回日本骨代謝学会  2005 

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  • 咬筋肥大症の患者に対して咬筋切除術と矯正治療を行った2症例

    第64回日本矯正歯科学会  2005 

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  • メカノセンサーとしての骨細胞における力学刺激応答のin vitro観察

    第30回複合材料シンポジウム及び第5回日韓ジョイント複合材料シンポジウム  2005 

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  • 実験的歯の移動時のカルシトニン遺伝子関連ペプチドと破骨細胞および骨芽細胞との三次元組織学的形態計測

    第64回日本矯正歯科学会  2005 

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  • 骨細胞の形態・形態計測について

    京都大学大学院工学研究科機械工学教室セミナー  2004 

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  • Distinct calcuim response of flow-induced primary bone cells

    International Symposium on Nano-Biotechnology & 6th International Conference on Protein Phosphatases  2004 

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  • 歯科矯正学の卒前教育における論述試験、技工物作成ならびに医療面接系OSCEの評価結果間の関連性

    日本矯正歯科学会  2004 

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  • 歯科矯正学客観的試験対策セミナーの効果について

    日本矯正歯科学会  2004 

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  • 流体剪断力を負荷した培養骨細胞は骨芽細胞、ライニング細胞に比べ細胞内カルシウム応答を示す細胞が少ない

    日本矯正歯科学会  2004 

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  • 骨芽細胞から骨細胞への最終分化に伴うアクチン結合蛋白の局在変化について

    日本エムイー学会秋季大会  2004 

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  • Calcium responses in flow-treated primary chick bone cells.

    第52回国際歯科研究学会日本部会(JADR)総会・学術大会  2004 

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  • Advanced osteocyte morphology and morphometry

    International congress of the IFAA  2004 

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  • 骨芽細胞から骨細胞への形質転換に伴うアクチン結合蛋白の局在変化について

    日本骨代謝学会  2003 

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  • Three-dimensional quantitative evaluation of osteocytes in bone

    IBMS&JSBMR  2003 

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  • 骨芽細胞から骨細胞への最終分化に伴うアクチン結合蛋白の局在変化について

    日本矯正歯科学会  2003 

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  • ニワトリ胚頭蓋骨中の骨細胞の三次元的形態計測

    日本矯正歯科学会  2003 

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  • 骨細胞の形態形成について

    新潟大学医学部整形外科学教室セミナー  2003 

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  • 前歯部歯冠補綴のための矯正治療について

    中・四国矯正歯科学会  2003 

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  • Three-dimensional quantitative evaluation of osteocytes in bone

    International Symposium Carduivascular Physiome  2003 

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  • Actin-binding proteins in osteocytes and osteoblasts

    International Symposium Carduivascular Physiome  2003 

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  • 骨芽細胞株MC3T3-E1細胞における流体剪断応力負荷時のCTGF発現とそのシグナル伝達機構の研究

    日本矯正歯科学会  2003 

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  • ニワトリ胚頭蓋骨中の骨細胞の三次元的形態計測

    日本骨代謝学会  2003 

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  • Transformation of osteoblasts to osteocytes is accompanied by the dramatic changes in the distribution of actin-binding proteins

    IBMS&JSBMR  2003 

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  • 共焦点レーザー顕微鏡及び高精細3D再構築ソフト(Imaris)を用いた骨細胞立体像の構築

    日本歯科基礎医学会総会  2002 

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  • 「蛍光色素を用いた骨細胞の形態的・機能的解析」

    長崎大学歯学部口腔病理学教室セミナー  2002 

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  • 蛍光色素を用いた骨細胞の形態的・機能的解析

    長崎大学歯学部口腔病理学教室主催セミナー  2002 

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  • The distribution of osteocyte processes revealed by confocal laser scanning microscopy and differential interference contrast microscopy

    Fourth Bone Fluid flow Meeting  2002 

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  • 共焦点レーザー顕微鏡と透過レーザー微分干渉顕微鏡を併用した骨細胞細胞突起の3次元的形態観察

    日本電子顕微鏡学会第58回学術講演会  2002 

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  • 「蛍光色素を用いた骨細胞の形態的・機能的解析」

    長崎大学歯学部口腔病理学教室主催学生特別講義  2002 

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  • 蛍光色素を用いた骨細胞の形態的・機能的解析

    長崎大学歯学部口腔病理学教室主催学生特別講義  2002 

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  • 共焦点レーザー顕微鏡及び高精細3D再構築ソフト(Imaris)を用いた骨細胞立体像の構築

    第76回日本矯正歯科学会  2002 

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  • Parry-Romberg(PR)症候群を伴う患者の頭蓋顔面・口腔の形態的特徴および機能的特徴

    第76回日本矯正歯科学会  2002 

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  • 骨細胞の3次元的形態観察

    学術会議解剖学研究連絡会議主催シンポジウム“Bone Cell Biology”  2001 

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  • Mechanical stimulation induces CTGF expression in rat osteocytes

    第19回 International Association of Dental Research & Japanease Association of Dental Research  2001 

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  • Anatomical and functional study of osteocytes using fluorescence

    ハンブルグ大学骨病理学教室主催セミナー  2001 

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  • Do the osteocytes in bone attach to their surrounding canaliculi and Lacuna?

    第1回 Joint meeting of International Bone and Mineral Society & European Caltified Tissue Society  2001 

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  • 骨細胞の三次元構築と形態計測

    バイオエンジニアリングサマーセミナー2001 (川崎医科大学医用工学教室主催)  2001 

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  • メカニカルストレスにより結合組織成長因子の発現が誘導され、その発現には細胞間コミュニケーションが関与している

    第19回日本骨代謝学会  2001 

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  • 下顎枝矢状分割術を行った骨格性下顎前突症例

    第44回中・四国矯正歯科学会  2001 

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  • Maxillary distraction osteogenesis in a preadolescent patient with skeletal Class III : a case report

    第60回 The Japanese Orthodontic Society 第3回国際会  2001 

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  • Gap junctional communication is important for CTGF expression in response to mechanical stress in MC3T3-E1

    第60回 The Japanese Orthodontic Society 第3回国際会  2001 

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  • Do the osteocytes in bone attach to their surrouding canaliculi and lacuna?

    第60回 The Japanese Orthodontic Society 第3回国際会議  2001 

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  • 多数歯先天欠如、劣成長を伴う上顎部にREDシステムを適用した仮骨延長の1症例ー術前・術後の形態的・機能的評価ー

    日本矯正歯科学会  2000 

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  • 多数歯先天欠如、劣成長を伴う上顎部にREDシステムを適用した仮骨延長の1症例ー術前・術後の形態的・機能的評価ー

    日本顎変形症学会総会  2000 

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  • 先天異常による不正咬合の治療における矯正歯科医の役割ー遺伝子診断とチームアプローチ

    日本歯科医学会総会  2000 

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  • 共焦点レーザー顕微鏡と透過レーザー微分干渉顕微鏡を併用した骨細胞細胞突起の3次元的形態観察

    日本骨代謝学会  2000 

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  • 上下顎骨切り術を行った骨格性下顎前突症例

    中・四国矯正歯科学会  2000 

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  • 共焦点レーザー顕微鏡と透過レーザー微分干渉顕微鏡を併用した骨細胞細胞突起の3次元的形態観察

    日本矯正歯科学会  2000 

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Awards

  • 学術大会優秀発表賞 (1件)

    2021.11   日本矯正歯科学会  

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  • 優秀ポスター賞(一般)

    2021.10   第77回日本顕微鏡学会学術講演会  

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  • 日本矯正歯科学会 学術大会優秀発表賞 (1件)

    2019.11  

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  • 学術大会優秀発表賞(5件)

    2018.11   日本矯正歯科学会  

    上岡 寛

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  • 優秀ポスター賞

    2018.7   日本骨代謝学会  

    上岡 寛

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  • 学術大会優秀発表賞(2件)

    2017.10   日本矯正歯科学会  

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    Country:Japan

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  • 学術大会優秀発表賞(2件)

    2016.11   日本矯正歯科学会  

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  • ANZBMS 2016 Travel Award

    2016.5   日本骨代謝学会  

    上岡 寛

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  • ASBMR 2016 Travel Award

    2016.5   日本骨代謝学会  

    上岡 寛

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  • Award of Excellence

    2015.11   日本矯正歯科学会(JOS)  

    上岡 寛

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  • 2015 Prenary Poster Award

    2015.9   ASBMR  

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  • 学術大会優秀発表賞(1件)

    2014.10   日本矯正歯科学会  

    上岡 寛

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  • 第29回日本骨形態計測学会 ゴールドリボン賞

    2009.6  

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  • Best Biological Paper Award 2007

    2008.1   アメリカ顕微鏡学会  

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    Country:Japan

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  • 日本矯正歯科学会学術大会優秀発表賞

    2007.10  

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  • 富士裾野フォーラム 奨励賞

    2006  

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  • 日本矯正歯科学会学術大会優秀発表賞

    2006  

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  • 日本矯正歯科学会学術奨励賞

    2006  

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  • JADR学術奨励賞

    2006  

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    Country:Japan

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  • 岡山歯学会 優秀論文賞

    2005  

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  • 日本骨代謝学会優秀ポスター演題賞

    2003  

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  • 日本骨代謝学会優秀ポスター演題賞

    2003  

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  • 学術大会優秀発表賞(日本矯正歯科学会)

    2000  

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Research Projects

  • Coevolution of facial formation due to changes in food and environment after the bottleneck of ethnic migration

    Grant number:20H05133  2020.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    上岡 寛

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    Grant amount:\5460000 ( Direct expense: \4200000 、 Indirect expense:\1260000 )

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  • HLA genome editing of dental pulp cells for iPS cell stock.

    Grant number:19K22707  2019.06 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)  Grant-in-Aid for Challenging Research (Exploratory)

    手塚 建一, 上岡 寛

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    Grant amount:\6500000 ( Direct expense: \5000000 、 Indirect expense:\1500000 )

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  • Elucidation of mechanosensor function acquisition process of osteocytes using 3D-volumetric image data

    Grant number:19H03859  2019.04 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    上岡 寛, 植田 紘貴, 早野 暁, 亀尾 佳貴, 原 徹

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    Grant amount:\17160000 ( Direct expense: \13200000 、 Indirect expense:\3960000 )

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  • 生体マルチモーダル解析を用いた骨形成・認知機能の複合的促進機構の解明

    Grant number:19K10405  2019.04 - 2022.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    植田 紘貴, 石原 嘉人, 亀田 雅博, 内部 健太, 上岡 寛

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

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  • 骨細胞性骨溶解を基軸とした歯牙移動制御機構の解明

    Grant number:17H04413  2017.04 - 2021.03

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    石原 嘉人, 飯村 忠浩, 早野 暁, 上岡 寛

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    Grant amount:\16770000 ( Direct expense: \12900000 、 Indirect expense:\3870000 )

    骨細胞性骨溶解は骨細胞が破骨細胞の様に骨基質を溶解し、生体にミネラルを供給するという概念で、骨代謝学領域で再注目されている事項である。本研究は、矯正学的歯の移動で起こる骨改造に骨細胞性骨溶解が及ぼす影響を生体バイオイメージングとマウスジェネティクスを用いて検討を行い、歯牙移動制御に関与する分子機構を明らかにする。そして、矯正歯科治療期間短縮への臨床展開を見据えた研究基盤の確立する事を目的としている。
    平成30年度実施計画では、平成29年度確立したマウスを用いた実験的歯の移動モデルを用い、骨細胞性骨溶解の変動について時空間的解析を行う予定であったが、進捗状況に若干の遅れが生じている。これは、遺伝子改変マウス作成の遅延に由来している。他方、本研究代表者は骨細胞への機械的刺激に対する変動遺伝子に関してGene Chip analysisを駆使し、その制御に関わるいくつかの候補遺伝子を発見した。これらの成果は日本骨代謝学会ならびに先端歯学スクールにて表彰され、結果の一部についてはJBMM誌に論文受理された。さらに、矯正学的歯の移動で起こる骨改造について、歯根膜からの細胞内カルシウムシグナルが骨のリモデリングに重要な役割をなす事を見出した。これらの結果については、現在論文投稿中である。今後は骨細胞特異的遺伝子改変マウスの作成を進める一方、上記研究で得られた研究結果と骨細胞性骨溶解との関連性について研究を発展させていく予定である。

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  • A Novel Method to Detect 3D Mandibular Changes Related to Soft-Diet Feeding

    Grant number:17K17325  2017.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    KONO Kana, TANIKAWA Chihiro, YAMASHIRO Takashi, KAMIOKA Hiroshi

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    Grant amount:\4030000 ( Direct expense: \3100000 、 Indirect expense:\930000 )

    Soft-diet feeding is a widely-used experimental model for studying the association between the skeletal morphology and muscle-related loading on the bone. Traditionally, these studies have been based on two-dimensional (2D) radiographs in the lateral view. However, 2D observation cannot detect three-dimensional (3D) changes in detail. Therefore, we have newly developed and reported a modified surface-based analysis using micro-3D computed tomography (CT) to examine and detect 3D changes of the mandible associated with soft diet feeding.

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  • Functional analysis of RPL5 using induced pluripotent stem cells from the patient with Diamond-Blackfan Anemia

    Grant number:17K17323  2017.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Hayano Satoru, SHIMADA Akira, SAITO Megumu, KAMIOKA Hiroshi, KAWANABE Noriaki, Fukui Yuko

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    Grant amount:\4030000 ( Direct expense: \3100000 、 Indirect expense:\930000 )

    In this study, we focused on Diamond-Blackfan anemia (DBA), which is characterized by red blood cell aplasia and craniofacial defects. iPS cells derived from DBA patient was used in this study. Our current study indicates that craniofacial defect is caused by activation of p53-mediated cell death pathway which is induced by interaction between ribosomal proteins and MDM2.

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  • 口蓋裂患者における鼻咽腔閉鎖率分析-VPIに対する治療基準の確立を目指して-

    Grant number:17K11937  2017.04 - 2018.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    村上 隆, 飯田 征二, 片岡 伴記, 上岡 寛

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    期間中に来院した口蓋裂患者の中から本研究の目的を理解し賛同する者を被験者とするため、被験者のリストの作成を図った(但し、未成年で保護者の同意が得られない者、発達障害を有する者、多動性障害を有する者、MRI 撮像により障害を被る可能性のある者、言語評価が得られない者、従来の鼻咽腔閉鎖機能の評価が行えない者は除外する)。
    データ採得を行う時期として、口唇裂・口蓋裂総合治療センターで通常診療時、音声言語評
    価および鼻咽腔閉鎖機能を評価する時期と設定した(不必要な検査を行うことはない)。すなわち、データ採得時期は初診時、治療前、(軟口蓋挙上装置の使用の場合治療中も)、および治療後とした。鼻咽腔形態の撮像に際しては、被験者に仰臥位で、設定した専用プロトコルに従い発声を指示し、60 秒間MRI movie を撮像するため、撮像装置としてMRI(3.0T Magnetom Verio, Siemens, Erlangen, Germany)を用い、Gradient Echo sequence により撮影を行うよう研究プロトコルを検討した。音声言語の評価としては、現在日本で広く実施されている2 種類の聴覚判定法(遠城寺式・乳幼児分析発達検査表(九州大学小児歯科改訂版)、新版発音検査(千葉テストセンター))を用いて岡山大学病院口唇裂・口蓋裂総合治療センター検査室にて評価した。そして、鼻咽腔閉鎖機能の評価には、従来より定量可能な検査法であるセファログラムとナゾメータ検査を行うこととした。そのため、ナゾメータを新たに購入し、検査体制を整えた。

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  • Roles of CCN2 and Rab14 in the formation of extracellular matrix

    Grant number:16K11786  2016.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Hoshijima Mitsuhiro

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    Grant amount:\4810000 ( Direct expense: \3700000 、 Indirect expense:\1110000 )

    CCN2 and Rab14 strongly expressed in bone, cartilage and lung. To elucidate the roles of CCN2 and Rab14 in osteocyte maturation, we investigated the different expression of osteocyte-related genes between young osteocytes and developmentally mature osteocytes using 3D-cultured MLO-Y4 cells. As the results, in the mature MLO-Y4 cells, rab14, ccn2, col1a1, OCN, c-Fos, Cx43 and Panx3 mRNA expression were significantly increased in comparison with young cells. Furthermore, in the present study, we showed for the first time that intracellular Ca2+ levels were significantly increased in developmentally mature osteocytes in comparison with young osteocytes by flow-induced mechanical stress.
    These findings show that the CCN2 and Rab14 plays an important role in the formation of extracellular matrix, and the intracellular Ca2+ responses in a 3D cellular network in osteocyte.

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  • Analysis of the new role of a collagen network for the morphogenesis during the bone modeling

    Grant number:16H05549  2016.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    KAMIOKA HIROSHI, ADACHI Taiji, JAGER Edwin

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    Grant amount:\17160000 ( Direct expense: \13200000 、 Indirect expense:\3960000 )

    We performed three-dimensional construction of collagen fibers in the osteogenic phase by using FIB-SEM. Furthermore, since this analysis extends to a cubic region of 25 micrometers per side, it was possible to capture a cellular network containing multiple osteocytes simultaneously. From these observations, processes extending from the osteoblasts to the matrix side have a certain regularity so as to avoid bundled collagen fibers. Furthermore, the bones pretreated with BAPN, which inhibits collagen fiber aggregation, cell processes extending from osteoblasts have no specific orientation. Thus, it has been revealed that changing the nature of bone matrix affects osteocyte network formation.

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  • The involvement of cell polarity in trabecular formation

    Grant number:16K15837  2016.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    KAMIOKA HIROSHI

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    Grant amount:\3510000 ( Direct expense: \2700000 、 Indirect expense:\810000 )

    A knockdown experiment of Nesprin - 1 using siRNA for osteoblast cell line Saos 2 was performed. By introducing Si - Nesprin 1, it was confirmed by immunofluorescence that nuclear localization of Nesprin decreased. Furthermore, it was confirmed that si-Nesprin 1 suppresses expression at the protein level by the Western blotting method. Therefore, it was confirmed that si-Nesprin 1 was introduced into Saos 2 cells.
    Next, culture experiments of knockdown cells using uneven surface structures were performed. As a result, there was no significant difference between the two in the arrangement of the cells. Next, in order to examine the change of Saos 2 in cell polarity in mechanical stress, cell extension experiment with flexer cell unit was performed. However, in Saos 2, no change in polarity to mechanical stress was observed, so Nesprin's involvement was not considered.

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  • 後天的に顔面骨格形態を決定する新たな分子メカニズムの解明

    Grant number:26463093  2014.04 - 2016.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    柳田 剛志, 上岡 寛, 久保田 聡

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    Grant amount:\4810000 ( Direct expense: \3700000 、 Indirect expense:\1110000 )

    現代社会では食生活の変化に伴い咀嚼機能が低下し、顎骨が退化縮小した結果、不正咬合が増加したと言われている。しかし、食生活などの後天的な影響がどのようにして顎態を変化させるのか、その分子機構は未だ不明である。今回我々はその分子機構を調査するため以下の実験を開始した。
    生後3週のICRマウスを2群に分け、粉餌、固形餌を与え5週齢まで飼育した。粉餌飼育群をさらに2群に分け、粉餌、固形餌を与え7週齢まで飼育した。その後同様に2群に分け9週齢まで飼育した。生後3週、5週、7週、9週の各時点においてサンプルを回収し実験を行った。小動物用X線CT装置にて撮影したマウス頭部を立体構築し、セファログラム分析法を応用してサンプル間の詳細な形態比較を行った。また各サンプルの咬筋からtotal RNAを回収し、筋機能を反映すると言われているMyh遺伝子群の発現をRealtime RT-PCR法により比較した。また、咬筋の筋線維特性を速筋と遅筋を分けることのできるNADH-TR染色を用いて観察し、顎骨の形態変化と遺伝子発現の変化及び筋線維特性の関連性を考察した。Micro-3DCTを用いてマウスの顎骨の形態を解析した結果、食餌の硬さの違いで下顎頭の高さ、歯槽骨の高さが変化した。また、Myh遺伝子群が食餌の硬さを変更させた後に応答し、発現を変化させていた。さらに、組織染色から筋線維の特性が変化した。マウスの顎骨の形態を詳細に解析した結果、粉末食を与えたマウスは骨格性ハイアングルの形態的特徴を有していた。特に生後3から7週の期間に与えた食餌の硬さが最終的な骨格形態に影響を与えることが示された。また、粉餌を与え続けたマウスでは硬餌を与えたマウスに比較してMyh4遺伝子の発現量が増加、Myh2遺伝子の発現量が減少し、これに伴い組織染色では速筋の比率が減少することが分かった。

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  • Search for new mechanism of bone remodeling by the collagen nano-model analysis

    Grant number:25293419  2013.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    KAMIOKA HIROSHI, KAMEO YOSHITAKA, SUMIYOSHI KUMI, HARA TORU, ADACHI TAIJI

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    Grant amount:\17290000 ( Direct expense: \13300000 、 Indirect expense:\3990000 )

    Osteocytes form the cellular network by their long processes and act as mechanosensory cells in bone. However, it is unknown how this cellular network is formed and what kind of factors influence the network formation. Focused ion beam-scanning electron microscopy (FIB-SEM) has increasingly found use in biological research. The main application is in the acquisition of three-dimensional data by tomography. We therefore reconstructed three-dimensional images of the osteocyte network as well as the collagen fibrils during bone modeling. Based on the positional relationships of osteocytes and collagen fibrils, we analyzed the influence of the collagen bundle formation on the osteocyte network formation.

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  • Novel functions of Runx genes as causal genes for ectodermal dysplasia

    Grant number:24249093  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    Yamashiro Takashi, NAKAGAWA ICHIRO

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    Grant amount:\45240000 ( Direct expense: \34800000 、 Indirect expense:\10440000 )

    In this study, we investigated the possible roles of epithelial Runx1/Cbfb signaling in the organogenesis and craniofacial development. Using the epithelial-specific Runx1 knock-out and epithelial-specific Cfbf knock-out mice, we provided evidence that Runx1/Cbfb has indispensable roles in the development of the teeth, the salivary gland, and the palate. In salivary gland, Runx1 is involved in sexual dimorphism in the induction of the granular convoluted tubules of the submandibular gland in the presence of androgen. In the incisors, Runx1 is involved in the regulation of Lgr5-positive epithelial stem cells that differentiate into ameloblasts, through regulation of phosphorylation of Stat3 in the cervical loop of the growing incisors. In the palate, Runx1 epithelial-specific deletion led to the failed disintegration of the contacting palatal epithelium and that Tgfb3 is a critical downstream target in the pathogenesis of anterior cleft palate in the mutants.

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  • THE EFFECT OF MYOSTATIN ON BONE REMODELING

    Grant number:24659909  2012.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    KAMIOKA HIROSHI, YANAGITA Takeshi

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    Grant amount:\3640000 ( Direct expense: \2800000 、 Indirect expense:\840000 )

    In order to examine the participation of muscle to the bone via humoral factor, we focused on the myostatin, which inhibits muscle growth. Firstly, we tried to examine the effect of myostatin on the bone formation. In the process of examining the bone formation, we could establish realtime calcium imaging of bone cells as well as 3D analysis of microstructure of bone.

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  • The possible role of endoplasmic reticulum stress in orthodontic tooth movement

    Grant number:24792283  2012.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    MURAKAMI Takashi, YANAGITA Takeshi, KAMIOKA Hiroshi

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    The detailed mechanisms of orthodontic tooth movement have not been revealed. On the other hand, it was revealed that endoplasmic reticulum stress induced that cell survival and cell necrosis. The aim of this study was to examine the participation of the endoplasmic reticulum stress in periodontal membrane and the alveolar bone using an experimental tooth movement. Because the endoplasmic reticulum stress marker expressed in bone addition and the hypoxia situation, it was suggested that endoplasmic reticulum stress participated in the orthodontic tooth movement.

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  • The elucidation of molecular mechanisms of amelogenesis by epitherial-specific Runx knockout mouse

    Grant number:22390390  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    HONJO Tadashi, YAMASHIRO Takashi, KAMIOKA Hiroshi

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    Grant amount:\19370000 ( Direct expense: \14900000 、 Indirect expense:\4470000 )

    Targeted deletion of Runx genes has revealed distinct roles for these proteins in development. Here, we report that epithelial Runx genes are involved in regulation of ameloblastdifferentiation. We analyzed the functional role of Cbfb and Runx1 in the enamel formation invivo, using a conditional genetic approach in which the Cbfb and Runx1 gene was inactivated in mouse epithelial cells(K14-Cre/Cbfbfl/fl, K14-Cre/Runx1 fl/fl). We found that mice deficient in epithelial Cbfb and Runx1 resulted in enamel aplasia in the incisors and in hypoplasia in the molars. The present results provide the genetic evidence that Runx1 and Cbfb genes function in the enamel formation in developing molar and incisors.

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  • Mechanical response in artificially controlled osteocyte network

    Grant number:21592594  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    KAMIOKA Hiroshi, SUGAWARA Yasuyo, KATANOSAKA Yuki

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    Osteocyte in bone are thought to be mechanosensory cells. They form cellular network. However, the role of osteocyte network is not well known. We employed the nano-patterning method to form artificially-formed osteocyte network and applied mechanical stress with fluid shear stress in micro-fluid flow chamber.

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  • New approach to the mechanism of the pain by orthodontic tooth movement

    Grant number:21592596  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    MURAKAMI Takashi, KAMIOKA Hiroshi, YAMASHIRO Takashi

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    Orthodontic tooth movement evokes a pain. As for the past research to the mechanism of the pain, the focus has been placed on neural cells. This research newly focused on glial cells surrounding neurons, and searched how glial cells involved in the development of the pain by orthodontic tooth movement. This research revealed not only temporal distribution of activated glial cells but also the involvement of CGRP and VR1 in central nervous system during orthodontic tooth movement.

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  • 歯の移動時における一酸化窒素(NO)の機能解明―NOSノックアウトマウスを用いて

    Grant number:20592402  2008 - 2010

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    湊 雅直, 山城 隆, 本城 正, 上岡 寛, 黒坂 寛, 藤井 昭仁

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    Grant amount:\3250000 ( Direct expense: \2500000 、 Indirect expense:\750000 )

    歯に矯正力が加わると、歯根膜組織で炎症様反応を含む初期反応が生じる。歯の移動の痛みや歯根吸収といった矯正治療に伴う副作用の多くは、この初期反応に関連することが示唆されるものの、その発生の詳細は不明である。歯根膜には間葉組織、血管、神経組織といった異なる組織が混在し、これらの相互作用を含む反応を理解することは容易ではない。そこで本研究では、歯の移動によるこの初期炎症反応を強力に誘導する一酸化窒素(NO)の役割の詳細を検討することを目的とした。本年度においては、NOSノックアウトマウスの表現型を検討するための予備実験としてC57BLマウスを用いて実験条件の検討を行った。これまでの歯の移動実験では、ほとんどの実験動物にラットが用いて行われており、本年度の研究の目的として、遺伝子改変マウスにおいて骨や歯槽骨での表現型を検討するための、実験条件の詳細な設定を試みた。その結果、歯の移動方法として、これまでラットの歯を移動させるために用いられたエラスティックと比べて、弾力が高く、非常に細い500μmの弾性スレッドを用いる方法を考案した。組織像から、歯に適切な矯正力が加わり、過度な骨や歯の吸収が生じることなく歯が移動することが確認された。この実験モデルを用いて、TRAP染色をおこなった結果、歯の移動開始、6時間後、12時間後、24時間後、72時間後に、TRAP陽性細胞数を確認し、骨吸収の動態を確認するのが適切であることが明らかとなった。特に、ラットにおける歯の移動モデルと異なり、歯の移動開始6時間後にはTRAP陽性細胞数が増加することを見出した。また、in situ hybridizationによって骨代謝マーカー遺伝子のmRNAの分布を検討した。これらの研究結果を元に来年度はNOSノックアウトマウスを用いて、TRAP細胞の出現と骨代謝マーカーmRNAの発現動態を元に表現型の解析を行い、歯の移動においてNOSが果たす役割を検討する予定である。

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  • 矯正的歯の移動における結合組織成長因子の役割-アポトーシスによる歯槽骨の改造-

    Grant number:18659614  2006 - 2007

    日本学術振興会  科学研究費助成事業 萌芽研究  萌芽研究

    山本 照子, 上岡 寛, 黒田 晋吾, 湊 雅直

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    Grant amount:\3300000 ( Direct expense: \3300000 )

    結合組織成長因子(CTGF)は、in vivoにおける機械的刺激において、骨細胞、骨芽細胞で遺伝子発現することが知られているが、その役割は明らかではない。一方、in vitroにおける機械的刺激によって、CTGFがヒト腎線維芽細胞で発現し、アポトーシスを誘導したとの報告がされている。本研究は、マウス実験的歯の移動モデルを作成し、in situハイブリダイゼーション法を用いて歯周組織におけるCTGF mRNAの発現を検討した。また、TUNEL染色及びISOL染色にてDNAの断片化を経時的に検索し、歯周組織に発現するCTGFが歯槽骨リモデリング時の細胞のアポトーシスに関与している可能性を検討した。
    機械的刺激による骨リモデリング時に、CTGF mRNAは骨細胞、骨芽細胞、破骨細胞に発現しており、CTGFが骨のリモデリングに関与していることが強く示唆された。CTGF mRNAを発現する骨細胞は、歯の移動開始から12時間後まで増加し、その後1日から21日まで経時的に減少した。すなわち、CTGF mRNAは歯の移動の機械的刺激が加わった初期において、歯槽骨圧迫側の骨細胞で一過性に多く発現することが明らかになった。また、歯槽骨圧迫側におけるTUNEL陽性骨細胞は、歯の移動開始から12時間後まで増加し、その後1日から21日まで経時的に減少した。この変化は骨細胞におけるCTGF mRNAの発現様相とほぼ一致していた。さらに、歯の移動12時間後では、歯槽骨圧迫側にISOL染色陽性を示す骨細胞が多く認められることから、骨細胞の細胞死はアポトーシスによると考えられる。以上のことから、実験的歯の移動時の骨細胞において、CTGFが発現しアポトーシスを誘導することによって、骨リモデリングに関与している可能性が示唆された。これらの結果は国際誌に投稿中である。

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  • Virtual bone reflecting microstructure ofreal bone

    Grant number:18592234  2006 - 2007

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    KAMIOKA Hiroshi, YAMAMOTO Teruko

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    Grant amount:\3880000 ( Direct expense: \3400000 、 Indirect expense:\480000 )

    Aim: We applied electron tomography to the osteocytes in bone in order to make virtual bone.
    Materials & Methods: Chick calvariae were desected from 16-day old chick embryo. The calvariae were treated by 1 mM collagenase and 5 mM EDTA and trimmed in 3x3-mm for further use. These sections were fixed with 3% paraformaldehyde for 24h, stained with 2% protargol solution containing copper beads for 48h as silver-staining, and embedded in paraffin. After these treatment, 2 μm sections were placed on fromvar coated cupper grids. For tomographic data collection, sections were dipped in a solution of colloidal gold for 1-2 min. Electron tomographic data collection was carried out with H-3000 ultra-high voltage EM (Hitachi) at 2 MV equipped with a 360 °-tilt specimen holder. Images were recorded on a Tietz Tem-Cam 4k x 4k-pixel F415S slow scan CCD camera (TVIPS) binned to 2k x 2k pixels. Alignment of the tilt series and reconstruction of 3D volumes from aligned images was performed by the weighted back-projection method by using a tomography software package IMOD.
    Results: Stereo-pair image of osteocyte was taken by ultrahigh-voltage electron microscopy with tilt +/-7 °. Osteocyte clearly appeared with numerous processes and brunches. The tomograms were generated from two +1-60 ° tilt series of a 2.0 μm section of chick calvaria and the microgram were taken 2 ° intervals. We could observe the series with 0.3 μm interval in depth of osteocyte tomography. The final pixel size on the specimen was 8 nm. We could distinguish the osteocyte cell body and their processes with high-density dots of silver on the surface of cell membrane.
    Conclusions: It is possible to observe silver-stained osteocytes in thick section (2 μm) by ultrahigh-voltage electron microscopy. In addition, high-resolution (8 nm) electron tomography was performed to the osteocytes in chick calvariae.

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  • Systematic study on the osteocyte network formation and their mechanical responsibility

    Grant number:17209064  2005 - 2007

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    YAMAMOTO Teruko, KAMIOKA Hiroshi, DEGUCHI Toru, SUGAWARA Yasuyo

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    Grant amount:\39780000 ( Direct expense: \30600000 、 Indirect expense:\9180000 )

    Aim: We examined mechanical property to analyze the change from osteoblasts to osteocytes. Furthermore, gap junctional intercellular communication among osteocytes in chick calvaria was examined using FRAP analysis. We established 3D cultures of osteoblast and osteocytes.
    Materials & Methods: Bone cells in embryonic chick calvariae and in isolated culture were identified using fluorescently labeled phalloidin and OB7.3, a chick osteocyte-specific monoclonal antibody. The elastic modulus of living cells was analyzed with Atomic Force Microscopy. Using Fluorescence Replacement After Photobleaching (FRAP) analysis we examined the effect of changes in pH_0, [Ca^<2+>]_e, and addition of PTH on GJIC in osteocytes in chick calvaria. Anti-Connexin43 (Cx43) immunolabelling was used to localize gap junctions.
    Results: The elastic modulus of peripheral regions of cells in all three populations was significantly higher than in their nuclear regions. The elastic modulus of the peripheral region of osteoblasts was 12053±934 Pa, that of osteoid osteocytes was 7971±422 Pa and that of mature osteocytes was 4471±198 Pa. Cx43 immunoreactivity was detected in most of the osteocyte processes. FRAP analysis showed dye-coupling among osteocytes. In untreated osteocytes, fluorescence intensity recovered 43.7±2.2% within 5 min after photobleaching. Pretreatment of osteocytes with 18 α-GA, significantly decreased. When pH_0 was decreased, fluorescence recovery significantly decreased. Conversely, when pH_0 was increased, fluorescence recovery was significantly increased. When [Ca^<2+>]_e was increased from 1 to 25 mM, fluorescence recovery was significantly decreased. In bone fragments exposed to 1.0 to 10 nM rPTH for 3 h, replacement of fluorescence was significantly increased. Chelating intracellular calcium ions affected GJIC regulation by [Ca^<2+>]_e and PTH. There were significant differences between actin and microtubule cytoskeletons in the process of MC3T3-E1 cells and primary osteocytes.
    Conclusions: There were dynamic changes in the mechanical property of elastic modulus and in focal adhesions of bone cells. Furthermore, our study showed for the first time that GJIC among osteocytes is regulated by the extracellular environment and by hormonal stimulation during bone remodeling. This method may be more biologically relevant to living bone than current methods. Osteoblasts processes may have a different functional role than the osteocyte dendritic network.

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  • Visualization of osteocyte differentiation in living bone

    Grant number:16390606  2004 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    KAMIOKA Hiroshi, TAKANO Teruko, FUKUNAGA Tomohiro

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    Grant amount:\14600000 ( Direct expense: \14600000 )

    First, we could succeeded to reconstruct osteocyte network in bone using IMARIS, NEURON TRACER, SURPASS software. We informed the results in international journal "BONE" in 2005. Precise information is in the paper. Briefly, we measured osteocyte morphometry in chick calvaria and also showed morphological differences between osteocytes and osteoblasts. This technique was applied to visualize living osteocytes in bone by using OB7.3 chick osteocyte specific antibody. Now, we are in the way to visualize osteoblasts and osteocytes in living bone.
    Besides the morphological differences between osteoblasts and osteocytes, we for the first time demonstrated significant difference between the cells in response to mechanical stress, especially in fluid shear stress. In the study, osteocytes were less respositive to fluid shear stress than osteoblasts. It was believed that osteocytes are the chief mechanosensory cells in bone.
    However, in our knowledge based on the present study, osteocyte showed less calcium response than osteoblasts. In addition, we elucidated the implication of different focal adhesion formation between the cells.
    Therefore, we now showed morphological and functional differences in osteocytes by the grant this time.

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  • 生骨組織中での骨細胞の機械的刺激応答能の検討

    Grant number:14704052  2002 - 2003

    日本学術振興会  科学研究費助成事業 若手研究(A)  若手研究(A)

    上岡 寛

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    Grant amount:\21190000 ( Direct expense: \16300000 、 Indirect expense:\4890000 )

    平成14年度の研究により共焦点レーザー顕微鏡を用いて骨組織中の生きた骨細胞を蛍光的に可視化できることを確認した。また、同様の方法を用いれば細胞内カルシウムインディケーターであるFluo3を骨組織中の骨細胞に取り込まし、細胞内カルシウムの動態を観察することが可能であると思われた。平成15年度は、まず、骨系細胞の力学的負荷による細胞内カルシウムの動態を検討するために、骨細胞、骨芽細胞、ライニング細胞を骨組織より取り出し培養し、流体剪断応力を負荷してこれらの細胞の細胞内カルシウムの変化を追跡した。これにより、骨系細胞の細胞カルシウムの濃度、オシレーションの頻度などの基礎的な細胞内カルシウムの動態を得た。その結果、骨細胞、骨芽細胞、ライニング細胞は、ともに流体剪断応力に応答することが明らかになったが、予想に反して骨細胞のカルシウム応答能は、他の細胞に比べて非常に低いことがわかった。細胞内カルシウムの変動は各群で有意差を生じなかった。これらの結果から、in vitroでの骨細胞は機械的応答能が低いことが予測された。また、生骨への機械的負荷は現在負荷装置を開発中であり、今後詳細な情報は得られるものと考えられている。得られた結果と今回のin vitroの結果を比較することにより、骨細胞のまわりの環境が細胞の機械的応答能に影響をあたえるかどうかを検討することができる。

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  • CTGF gene delivery for enhancement the healing of mandibular ramus fracture

    Grant number:13672152  2001 - 2002

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    KURODA Shingo, KURATANI Tsuyoshi, KAMIOKA Hiroshi, TAKANO Teruko

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    Grant amount:\3900000 ( Direct expense: \3900000 )

    The purpose of the present study was to investigate the CTGF gene delivery for enhancing the repair of fractured condylar and mandibular ramus. Using six-week-old wistar rat, 12 hours, 1, 3, 5, 7, and 14 days after the fracture, mandible including fractured condylar and ramus were dissected and decalcified with EDTA. After embedded in paraffin, 5 μ, β horizontal and sagittal sections were made. RNA probes of CTGF, collagen type I, II, III, X, Cbfal and TGF 0 were used for in situ hybridization to examine the expression of these factors during fracture healing. CTGF were expressed not only hypertrophic but hyperplastic chondrocyte in the healing site and this expression was changed in the time course. The present findings suggest that CTGF may play an important roll for repair and healing in condylar fracture and this model is useful as a model of fracture healing.
    Following experiments were performed in vitro and in vivo, to examine the optimum conditions of HVJ liposome method for the condylar fracture model. (1) Luciferase gene was delivered to mesenchymal cell, isolated from the bone marrow in mouse femur, by using HVJ-liposome method in vitro. Luciferase reporter assay was performed to evaluate delivery rate. (2) After the fracture, β-galactosidase gene was delivered by using HVJ-liposome method. Three days after gene introduction, X-gal staining was performed to evaluate the delivery rate. These pilot studies indicated that the gene delivery by using HVJ-liposome was efficient to the condylar fracture model.
    CTGF gene delivered by using HVJ-liposome in rat is performed and both in situ hybridization and immuno-histochemistry were used to analyze the expression of CTGF during fracture healing. We are preparing the paper about these observations to submit to the international journals, and some of them have been accepted.

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  • Study on the mechanism for the periodontal ligament tissue regeneration using human periodontal ligament tissues derived from complex odontoma

    Grant number:13672151  2001 - 2002

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    TSUBOI Yoshiko, MIYAMOTO Manabu, KAMIOKA Hiroshi, TAKANO Teruko

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    Grant amount:\3900000 ( Direct expense: \3900000 )

    Orthodontic tooth movement depends on the active periodontal ligament remodeling and regeneration. To analyze this mechanism, we utilized odontoma PDL and tried to find the genes specific in odontoma PDL. Human PDL tissue derived from a premolar complex odotoma was excised, which was extracted from orthodontic reasons, and placed onto flexible bottom plate. One month later, the heterogeneous cells reached confluence and they were cloned. Each clone was collected at 6-8 passages and stocked. Cell morphology and growth and differentiation ability of each clone is analyzing.
    Total RNA was extracted from the heterogeneous primary culture cells from the odontoma PDL and from normal tooth PDL. To investigate the difference in the expression of Msx-1, Msx-2, which has been reported to control the tooth development, in odontoma PDL and normal tooth PDL, total RNA was reverse transcribed into cDNA, followed by quantitative real-tune PCR, using specific primers. As a result, all RNAs expressed the Msx-1 and Msx-2 mRNAs, though the expression level varied with the cells. The morphology of the cells also differed from each other.
    Furthermore, another periodontal ligament tissues from another complex odontoma were used and analyzed the expression of Msx-1 and Msx-2 and the same tendency was observed. These results suggested that the process for the formation of odontoma involves at least Msx-1 and Msx-2, but also other transcriptional factors. We are analyzing cDNA array method and differential display PCR method, to identity the genes that is expressed specific in odotoma PDL. We are also preparing to immortalize the cloned cells, and to identify the clone specific genes.

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  • Studies for linage of periodontal ligament cells by gene transfection technique.

    Grant number:12671997  2000 - 2001

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    MIYAMOTO Manabu, TAKANO Teruko, KAMIOKA Hiroshi

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    Grant amount:\3000000 ( Direct expense: \3000000 )

    To clarify a lineage of cells isolated from periodontal ligament (PDL), the following studies were undertaken. First, we constructed (he experimental protocol for introducing and expression of the foreign genes into primary culture of human PDL cells. We constructed the plasmid vector which have human telomerase gene at downstream for cytomegarovirus immediate early enhancer and promoter, and introduced it to the primary cell culture which were established by the outgrowth method from human PDL. The expression of telomerase gene in cloned cells selected by an antibiotic marker was confirmed by RT-PCR. Secondly, expression of alkaline phosphatase (ALP) which were known to be a classical marker for cells in osteoblastic lineage were analyzed in single cell derived-clones form human PDL cells. The cloned cells showed same morphology, however expressed different levels of ALP activities and showed different responses against dexamethasone. The RNA expression levels for ALP gene were almost identical among these clones, suggesting that the different levels for ALP activities were derived from translational and/or transmembrane process of ALP protein. Thirdly, we investigated the gene-expression profiles in different clones for human PDL cells. Total RNAs expressed in two different PDL clones, one is high ALP clone and other is low ALP clone, were analyzed by a microarray assay, and compared with each other in over 1,500 different genes. These PDL clones showed almost same RNA expression profiles and showed slight difference in several specific genes. Finally, we also analyzed the roles of nerve growth factors in mouse PDL clones.

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  • The role of cytoskeleton on the transaction of mechanical stress to biological response in osteocytes -Transfection of GFP gene to cytoskeleton

    Grant number:12671998  2000 - 2001

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    KAMIOKA Hiroshi, MIYAMOTO Manabu, TAKANO Teruko

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    Grant amount:\600000 ( Direct expense: \600000 )

    Morphological changes of cytoskeleton in osteoblasts and osteocytes were investigated after subjection to fluid shear stress. It was observed that osteoblastic cell line MC3T3-E1 react to fluid shear stress and changes its cytoskeleton (especially, actin) after application of fluid shear stress of 12 dynes/cm2 for 1 hour. Hence, other cytoskeletal protein, such as vimentin and tubulin did not - change their morphology after adaptation of fluid shear stress. When the cells were microinjected with fluoresncence-labeled vinculin and subjected to fluid shear stress, it was confirmed that the assembly of vinculin was performed within 10 minutes after application of fluid shear stress. So, it was suggested that morphological changes after fluid shear stress happens very rapidly. However, when osteocytes were subjected to fluid shear stress, the cytoskeletal changes was not observed. Based on these results, the cytoskeleton in osteocytes was very stable comparing to osteoblasts. Next, we focused on the stability of osteocyte morphology and observed the three dimensional distribution of osteocyte processes in bone using confocal microscopy and differential interference contrast microscopy.
    Furthermore, we found that connective tissue growth factor is a candidate of marker of cytoskeletal changes in bone cells.

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  • Analysis of genes induced in human periodontal ligament cells respond to the mechanical stress

    Grant number:12470460  2000 - 2001

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    TAKANO Teruko, TSUBOI Yoshiko, MIYAMOTO Manabu, KAMIOKA Hiroshi

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    Grant amount:\13800000 ( Direct expense: \13800000 )

    Human PDL tissue was excised from a premolar or a third molar, which were extracted from orthodontic reasons, and placed onto flexible bottom plate with Dalbecco's modified eagle medium (DMEM) containing 10 % fetal bovine serum. After 10 to 14 days fibroblastic cells were extended and after 1 or 3 months later, tension force was applied on the primary culture cells for 24h. The cells derived from the PDL tissue from the same patient without stress were used for control. Total RNA was extracted from both cells and reverse transcribed into cDNA, followed by PCR. The difference of the expression level of 1176 kinds of genes was analyzed by Micro Array method. Same experiments were done with other 3 patient's periodontal tissue and mRNA was collected at some points from early stage to late stage after mechanical stress. The genes reinforced by the mechanical stress, and those suppressed were identified. Some genes expressed strongly in early stage transiently, that decreased with time, and some showed reverse pattern. On the other hand, the mechanical tension force was applied using the cells that were subcultured to 3 passages. The results were almost the same to the experiments using the primary culture cells, although the number of genes responded to the stress were less and the amount of the expression change by stress was smaller. These results suggested that the aging accompanied with the long culture days or subculture lowed down the sensitivity for the mechanical stress or caused the selection for the cells that cannnot respond to the stress.
    Now, we are preparing the paper about these observations to submit to the international journals, and some of them have been accepted.

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  • THE ELUCIDATION OF THE FUNCTION OF THE OSTEOCYTES AS CALCIUM SENSORY CELLS

    Grant number:10671937  1998 - 1999

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    MIKI Yoshiki, KAMIOKA Hiroshi, HIURA Kenji, MORIYAMA Keiji

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    Grant amount:\2100000 ( Direct expense: \2100000 )

    Recently, a calcium-sensing receptor (CaSR) from bovine parathyroid tissue was cloned, and Northern blot analysis revealed expression of CaSR mRNA in several tissues other than the parathyroid gland. In the present study, we fractionated bone cells from 1-to 2-day-old rat calvaria by digestion with collagenase and EDTA and examined the expression of CaSR mRNA in cells of the various fractions by reverse transcription polymerase chain reaction (RT-PCR) analysis and in situ hybridization histochemistry. Fraction I cells had an elongate shape, and cells from fraction II through IV had a stellate and polygonal shape. Cells from fraction VI were small and stellate-shaped, and possessed a large number of long cytoplasmic processes. Fraction m cells expressed alkaline phosphatase (ALPase) activity and osteocalcin (Osc) mRNA, suggesting them to be osteoblasts. On the other hand, only fraction vl cells expressed CaSR mRNA ; and they displayed no ALPase activity or Osc mRNA. Furthermore, fraction VI cells responded to the elevated extracellular calcium with a rapid elevation of their cytosolic calcium. The phenomenon was independent of membrane voltage and insensitive to organic calcium channel modulators, such as BAY K 8644, nifedipine, and nicardipine. These findings strongly suggest that rat mature osteocytes express a CaSR that responds to elevated extracellular calcium.

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  • BIOLOGICAL CONTROL OF OSTEOCLASTS AND OSTEOBLASTS

    Grant number:07557133  1995 - 1996

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    SUMITANI Koji, HIURA Kenji

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    Grant amount:\7500000 ( Direct expense: \7500000 )

    In this study, we examined the biological controls of osteoblasts to osteoclasts in the activation and formation. To examine them, we used a culture system as follows,
    (1) unfractionated cells obtained from mouse long bones for osteoclastic formation
    (2) stromal cell free hematopoietic blast cells (mouse) for multinucleated cell formation
    (3) cloned mouse osteoblastic cells (MC3T3-E1)
    The results were obtained as follows,
    1) The conditioned medium (CM) of MC3T3-E1 cells precultured for 3days contained only the inhibitory factor of multinucleated cell formation. On the other hand, both inhibitory factor and stimulatory factor were found in the CM of MC3T3-E1 cells precultured for 60days. No such effectors were found in the CM of fibroblasts.
    2) The production of inhibitory and stimulatory factors were depressed by indomethacin and cycloheximide, respectively.
    3) The media of MC3T3-E1 cells precultured for 3 and 60days contained prostaglandin E2 which inhibited multinucleated cell formation.
    4) 1alpha, 25 (OH)_2D_3 was essential for stimulation of MNC formation by both MC3T3-E1 cells derived stimulatory factor and GM-CSF.
    These results suggest that the inhibitory factor is prostaglandin E2 and the stimulatory factor is GM-CSF like protein.
    5) Although TGF-beta1 has potent inhibitory effect on osteoclastic bone resorption, in the presence of 1alpha, 25 (OH)_2D_3, TGF-beta1 stimulated the formation of osteoclast.
    6) TGF-beta1 inhibited multinucleated cell formation induced by 1alpha, 25 (OH)_2D_3, but the conditioned medium of TGF-beta1-treated MC3T3-E1 cells stimulated such formation.
    7) Both bafilomycin A_1 (H^+-ATPase inhibitor) and acetazolamide (carbonic anhydorase II inhibitor) inhibited osteoclastic bone resorption stimulated by parathyroid hormone.
    On the other hand, bafilomycin A1 did not affect procathepsin L secretion while acetazolamide inhibited the secretion.
    These findings suggest that osteoblasts have the effect on osteoclastic formation through calcium regulating factors such as prostaglandin E2, GM-CSF and TGF-beta1.

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  • THE EFFECTS OF BONE MATRIX PROTEINS ON OSTEOCLAST FORMATION

    Grant number:06454584  1994 - 1995

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (B)  Grant-in-Aid for General Scientific Research (B)

    HIURA Kenji, KONDO Kahori, KAMIOKA Hiroshi, SUMITANI Koji

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    Grant amount:\800000 ( Direct expense: \800000 )

    Osteoclasts are multinucleated cells derived from hematopoietic precursors that have the unique ability to excavate bone matrix. In cultured rat marrow cells with bone particles (100mug/cm^2 ; 20-53mum), osteoclast-like cell number and bone resorbing activity increased by 1.5- and 30-fold, respectively. Recent observations on mice engineered to have targeted knockout of the c-src gene resulted in a phenotype bearing a recessive form of osteopetrosis, a consequence of a decrease in osteoclast function. since osteoclasts were still present in the homozygous src-mutants, it was postulated that the defect could be with the osteoclast itself. Therefore, we have used Chicken osteoclasts as a model system to study of signal transduction. Activation of osteoclasts by bone particles results in elevated tyrosine phosphorylation of three proteins, p200, p130, and p85. By immunoprecipitation and western blotting technique, we demonstrated that p85 was identified with Cortactin. This tyrosine phosphoprotein is a previously characterized cytoskeletal substrate for c-src in transformed cells. The data suggested that Cortactin was one of the members for src dependent signal transduction in activated osteoclast.
    Furthermore, we demonstrated that cytosolic calcium elevation that occurred in osteocytes on exposure to elevated extracellular calcium was independent of membrane voltage and was insensitive to modulation by organic calcium channel modulators, namely, BAY K 8644, nicardipine, and nifedipine. However, an intracellular calcium antagonist such as TMB-8 affected the cytosolic calcium level, suggesting that the cytosolic calcium elevation was due to mobilization of this cation from an intracellular store.

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  • PARTICIPATION OF CATHEPSIN L ON ORTHODONTIC TOOTH

    Grant number:06672056  1994 - 1995

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (C)  Grant-in-Aid for General Scientific Research (C)

    SUMITANI Koji, TAGAMI Kahori, HIURA Kenji

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    Grant amount:\2200000 ( Direct expense: \2200000 )

    Osteoclasts are multinucleated giant cells responsible for the resorption of the calcified extracellular bone matrix. The degradation of the bone matrix protein including type I collagen is a key process for osteoclastic bone resorption. It has been suggested that lysosomal cysteine proteinase in osteoclasts is essential to degrade bone collagen, but the proteinase(s) for this collagenolysis have not been identified.
    In this study, the participation of cathepsin L on osteoclastic bone resorption was investigated and the results obtained were as follows.
    1) In cultured rat bone marrow cells, parathyroid hormone (PTH)-stimulated bone resorption was markedly suppressed by all of specific and non-specific inhibitors of cathepsin L tested but not by a specific inhibitor (CA-074) of cathepsin B.
    2) In rats with bone resorption stimulated by a low calcium diet, serum calcium level was decreased by an intraperitoneal injection of cathepsin Linhibitors but not by CA-074.
    3) Activities of cathepsin L and B were shown to be more than 80% of total proteinase activity in the culture medium of rat bone marrow cells.
    4) In bone marrow cells cultured on bone slices, PTH increased both bone resorption and cathepsin L activity in the medium and these increases were suppressed by calcitonin.
    5) The cultured bone marrow cells secreted cathepsin L as 39kD molecule and the secretion was suppressed by calcitonin.
    6) The 39kD cathepsin L purified from rat long bones was converted to 25kD cathepsin L under acidic condition.
    7) Rat long bone-derived 25kD cathepsin L as well as purified cathepsin L of rat liver degraded type I collagen at pH 4.05.0.
    These results suggested that cathepsin L secreted from osteoclast plays a crucial role in the degradation of bone matrix proteins including type I collagen during bone resorption.

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  • 骨細胞のカルシウムの取り込みについて

    Grant number:06772009  1994

    日本学術振興会  科学研究費助成事業 奨励研究(A)  奨励研究(A)

    上岡 寛

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    Grant amount:\900000 ( Direct expense: \900000 )

    矯正的歯牙移動に伴う骨リモデリングは、破骨細胞による骨吸収および骨芽細胞による骨形成によってなされている。しかし、成熟した骨組織中には、これら細胞の約10倍もの骨細胞が占めている。しかし、骨細胞は回りを硬組織にとり囲まれているため単離ができず、その機能については、いまだ明らかにされていない。
    最近、我々は、ニワトリ胚頭頂骨より非常に高純度で(95%)で、多量(1匹当たり10,000個)の骨細胞を単離することができた(Isolation Method of Osteoidosteocytes .J Bone Miner Res:S202.1993)。この骨細胞を用いて、骨リモデリングにおける骨細胞の役割を探っていくことは、意義のあることと思われる。
    【結果】 骨リモデリング時に骨吸収側では、細胞外カルシウム濃度が生理的濃度の約20倍の40mMにも上昇することが報告されているが、この高濃度のカルシウムに骨細胞が応答し、骨細胞が骨代謝を調節するメカニズムついて検討を行うために以下の実験を行った。
    1)本学にあるACAS570 WORK STATIONをもちいて、まず単離した骨細胞の細胞内カルシウムの変化を指標として、細胞外カルシウムに対する骨細胞の応答を観察した。その結果、骨細胞は細胞外液のカルシウムに濃度依存的に応答することがわかった。
    2)骨細胞内のカルシウムの上昇がどのような機序によってなされているかを検討するために、膜電位依存性カルシウムチャンネルアゴニストおよびアンタゴニストを用いて細胞内カルシウムの変動を追った。アゴニストであるBAY K8644は細胞内カルシウムを上昇することはなかった。また、アンタゴニストであるnicardipine,nifedipineは細胞外カルシウムによって惹起される細胞内カルシウムの上昇を抑制することができなかった。
    3)また、二価の陽イオンであるニッケルおよびカドミウムは、カルシウムによって惹起される骨細胞の反応と競合し、外液カルシウム非存在下でも単独に細胞内カルシウムを上昇することがわかった。
    これらのことから骨細胞は細胞外液にカルシウムチャンネル以外の経路を介して応答していることが示唆された。

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  • 矯正力を作用した骨細胞が骨芽細胞の増殖および機能に及ぼす影響について

    Grant number:05454556  1993

    日本学術振興会  科学研究費助成事業 一般研究(B)  一般研究(B)

    河田 照茂, 石川 啓詞, 上岡 寛, 住谷 光治

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    Grant amount:\6900000 ( Direct expense: \6900000 )

    矯正力の作用機序を解明する上で、骨細胞に対する矯正力の影響を検討することは有用であると思われる。そこで、培養した骨細胞を用いて以下の実験を行った。
    実験方法
    閉鎖培養皿(ローズチャンバー)上に骨細胞を培養し静水圧を負荷し、矯正力を加えた骨細胞のモデル型を想定した。静水圧の調整はローズチャンバーに連結した点滴の高さを調整することにより行った。0, 25,100g/cm^2の各圧で24時間0.1%BSA含有α-MEMで培養された培養上清を回収し骨芽細胞培養系(MC3T3-E1細胞)に添加した。
    結果及び考察
    1.矯正力を負荷した骨細胞の培養上清が骨芽細胞の増殖に与える影響
    Meyer J.E.& Grundman H.の方法により、MC3T3-E1細胞中のDNA量を測定したところ、矯正力を負荷した骨細胞の培養上清は、骨芽細胞数の多少の増加を促すものの有意差は認められなかった。
    2.矯正力を負荷した骨細胞の培養上清が骨芽細胞のALP活性に与える影響
    ALP活性は、100g/cm^2の培養上清において未処理対照に比べ増加傾向がみられた
    以上の結果より矯正力を受けた骨細胞からは骨芽細胞の分化を促すような液性因子が産生されている可能性が示唆された。

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  • 矯正力が骨の細胞の接着機構に与える影響について

    Grant number:05671712  1993

    日本学術振興会  科学研究費助成事業 一般研究(C)  一般研究(C)

    住谷 光治, 石川 啓詞, 上岡 寛, 河田 照茂

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    Grant amount:\2000000 ( Direct expense: \2000000 )

    破骨細胞は骨基質に存在するArg-Gly-Aspの3つの連続するアミノ酸を認識して骨基質へ接着するインテグリンが存在することが知られている。我々の興味の対象はメカニカルストレスを負荷された骨の細胞が破骨細胞の基質への接着に与える影響である。本研究は、これによって歯科矯正学的な歯の移動のメカニズムの一端を知ろうというものである。
    1. マウス骨芽細胞株MC3T3E-1細胞とニワトリ卵より採取した骨細胞を各々培養し、静水圧(10-20g/cm^2)および培養面が軟膜でできた培養皿を変形させて物理的圧刺激(メカニカルストレス)を加えた。
    (1)24時間静水圧を負荷した場合には、MC3T3E-1細胞および骨細胞ともにおおきな形態変化は見られなかった。48時間負荷によってMC3T3E-1細胞はコントロール群と比較して星形から多少、紡錘形に変化した。
    (2)圧刺激を加えたMC3T3E-1細胞培養上清中に明らかにプロスタグランディンE_2が産生されていた。プロスタグランディンE_2を外因性に骨細胞に作用させると形態が比較的速やかに変化した。
    (3)(2)の培養上清を2次元電気泳動で展開すると明らかに静水圧、物理的圧刺激負荷群でコントロール群と比較して新しい蛋白性産生物が見られた。
    2. 24時間静水圧を負荷したMC3T3E-1細胞の培養上清を濃縮し、プラスチックディッシュ上のラット由来単離破骨細胞に与えても形態や剥離に対する影響は見られなかった。
    以上より、矯正力によって骨芽細胞や骨芽細胞影響を受けプロスタグランディンE_2以外の蛋白性物質を産生促進させるが、それが破骨細胞のインテグリンに直接は影響してはいないことが分かった。

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