論文 - 曲　正樹

Regulatory role for complement receptors (CD21/CD35) in the recombination activating gene expression in mouse peripheral B cells

Masaki Hikida, Masaki Magari, Yasunori Nakayama, Naoki Kanayama, Hitoshi Ohmori

Memoirs of the Faculty of Engineering Okayama Univiersity   36   51 - 60   2002年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Use of secondarily revised VH genes in IgE antibodies produced in mice infected with the nematode Nippostrongylus brasiliensis 査読

N Kanayama, C Hukue, M Magari, K Ohtani, M Hikida, M Yamada, S Matsuda, H Ohmori

IMMUNOLOGY LETTERS   77 ( 3 )   181 - 186   2001年7月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE BV  

Although a high level of IgE is produced after primary infection with Nippostrongylus brasiliensis (Nb), most of the IgE antibodies (Abs) are not specific to the worm. Analyses with Western blotting and enzyme-linked immunosorbent assay (ELISA) revealed that the IgE Abs from Nb-infected BALB/c mice did not show reactivity with Nb-derived excretory-secretory proteins (NES) and antigens present in the cell-free extracts of the worm. Monoclonal IEE Abs obtained from the Nb-infected mice were not reactive with these Nb antigen either. To characterize Nb-induced IgE response, we used (QM x C57BL/6)F1 (QBF1) mice that bear the knock-in 17.2.25 VHDJH segment (VHT) encoding a VH region specific to 4-hydroxy-3-nitrophenylacetyl hapten, and express VHT-encoded antigen receptors on 80-85% of their B cells. Consistent with the frequency of VHT-positive B cells, more than 80% of IgE Abs induced in QBF1 B cells that were cultured with LPS plus IL-4 were found to bear VHT-encoded H chains. In contrast, when QBF1 mice were infected with Nb, less than 10% of Nb-induced IgE Abs were found to use VHT. The QBF1-derived IgE did not react with Nb antigens either. Taken together, data suggest that Nb-induced IgE response in mice is not merely the result of polyclonal activation of B cells, but may involve a mechanism that revises Ig genes secondarily. (C) 2001 Elsevier Science B.V. All rights reserved.

DOI： 10.1016/S0165-2478(01)00216-4

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