2024/02/02 更新

写真a

エクニ ダイスケ
江國 大輔
EKUNI Daisuke
所属
医歯薬学域 教授
職名
教授
外部リンク

学位

  • 博士(歯学) ( 岡山大学 )

研究キーワード

  • Preventive Dentistry

  • 予防歯科学

研究分野

  • ライフサイエンス / 社会系歯学

学歴

  • 岡山大学    

    - 2002年

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    国名: 日本国

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  • 岡山大学   Graduate School, Division of Dental Research   Preventire dcntistry

    - 2002年

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  • 岡山大学   Faculty of Dentistry  

    - 1998年

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  • 岡山大学   Dental School   School of Dentistry

    - 1998年

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    国名: 日本国

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経歴

  • 岡山大学   学術研究院医歯薬学域予防歯科学分野   教授

    2023年8月 - 現在

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    国名:日本国

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  • 岡山大学   学術研究院医歯薬学域予防歯科学分野   准教授

    2021年4月 - 2023年7月

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    国名:日本国

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  • 岡山大学   大学院医歯薬総合研究科予防歯科学分野   准教授

    2017年5月 - 2021年3月

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  • - Associate Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2017年

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  • - 岡山大学医歯薬学総合研究科 准教授

    2017年

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  • Senior Assistant Professor,University Hospital of Medicine and Dentistry,Okayama University

    2012年 - 2017年

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  • 岡山大学   Okayama University Hospital

    2012年 - 2017年

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  • Researcher

    2006年

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  • ブリティッシュコロンビア大学歯周病学講座 研究員

    2006年

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  • 岡山大学医歯薬学総合研究科 助教

    2004年 - 2012年

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  • Assistant Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2004年 - 2012年

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  • Researcher

    2004年 - 2005年

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  • ブリティッシュコロンビア大学歯周病学講座 研究員

    2004年 - 2005年

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▼全件表示

所属学協会

▼全件表示

委員歴

  • 近畿・中国・四国口腔衛生学会   常任幹事  

    2023年8月 - 現在   

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    団体区分:学協会

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  • 岡山歯学会   評議員  

    2018年4月 - 現在   

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    団体区分:学協会

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論文

  • Effect of oral health status and oral function on malnutrition in community-dwelling older adult dental patients: A two-year prospective cohort study. 国際誌

    Nanami Sawada, Noriko Takeuchi, Daisuke Ekuni, Manabu Morita

    Gerodontology   2023年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES: To survey oral status in detail and clarify the factors associated with nutritional status as evaluated by the Mini Nutritional Assessment. BACKGROUND: Malnutrition is common in older adults and increases the risk of frailty, sarcopenia, and mortality. However, few longitudinal studies have been performed to investigate the causal effect of oral status on malnutrition in detail, and the information reported in those longitudinal studies has been limited to dentition status and swallowing ability. MATERIALS AND METHODS: The participants in this prospective cohort study were patients who visited the Preventive Dentistry Clinic at Okayama University Hospital from November 2017 to January 2021. The number of teeth, periodontal condition, oral function (oral diadochokinesis, masticatory ability, number of bacteria in tongue coating, occlusal force, oral moisture, tongue pressure, swallowing function), nutritional status, the number of comorbidities, and mental health status were examined. These variables were analysed using structural equation modelling (SEM). RESULTS: The final analysis included 141 participants (40 males and 101 females; mean age, 73.5 ± 6.3 years; follow-up rate: 79.7%). The results of the SEM revealed that oral status (number of teeth present and swallowing function) was positively associated with nutritional status 2 years later. In addition, age was negatively associated with oral status. CONCLUSION: In community-dwelling older adult dental patients (age ≥60 years), oral function at baseline was associated with nutritional status two years later.

    DOI: 10.1111/ger.12718

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  • Nutrients Associated with Sleep Bruxism. 国際誌

    Naoki Toyama, Daisuke Ekuni, Daiki Fukuhara, Nanami Sawada, Miho Yamashita, Momoe Komiyama, Takahiko Nagahama, Manabu Morita

    Journal of clinical medicine   12 ( 7 )   2023年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: The purpose of the present research was to identify nutrients related to sleep bruxism and to establish a hypothesis regarding the relationship between sleep bruxism and nutrients. METHODS: We recruited 143 Japanese university students in 2021 and assigned them to sleep bruxism (n = 58) and non-sleep bruxism groups (n = 85), using an identical single-channel wearable electromyography device. To investigate nutrient intakes, participants answered a food frequency questionnaire based on food groups. We assessed differences in nutrient intakes between the sleep bruxism and non-sleep bruxism groups. RESULTS: Logistic regression modeling showed that sleep bruxism tended to be associated with dietary fiber (odds ratio, 0.91; 95% confidence interval, 0.83-1.00; p = 0.059). In addition, a subgroup analysis selecting students in the top and bottom quartiles of dietary fiber intake showed that students with sleep bruxism had a significantly lower dietary fiber intake (10.4 ± 4.6 g) than those without sleep bruxism (13.4 ± 6.1 g; p = 0.022). CONCLUSION: The present research showed that dietary fiber intake may be related to sleep bruxism. Therefore, we hypothesized that dietary fiber would improve sleep bruxism in young adults.

    DOI: 10.3390/jcm12072623

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  • Association between oral condition and subjective psychological well-being among older adults attending a university hospital dental clinic: A cross-sectional study. 国際誌

    Noriko Takeuchi, Nanami Sawada, Daisuke Ekuni, Manabu Morita

    PloS one   18 ( 11 )   e0295078   2023年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Positive psychological well-being has a favorable impact on survival rates in both healthy and unhealthy populations. Oral health is also associated with psychological well-being, is multidimensional in nature, and includes physical, psychological, emotional, and social domains that are integral to overall health and well-being. This study aimed to identify the associations between individual and environmental characteristics, oral condition and nutritional status in relation to subjective well-being among older adults using the Wilson and Cleary conceptual model. The participants were older adults (age ≥ 60 years) attending a university hospital. Subjective well-being was assessed using the World Health Organization-5 Well-Being Index, oral condition was assessed based on the number of bacteria in the tongue coating, oral wettability, tongue pressure, occlusal force, oral diadochokinesis, and masticatory ability, and subjective swallowing function was assessed using the Eating Assessment Tool, number of remaining teeth, and number of functional teeth. In addition, factors related to well-being, including social networks, life-space mobility, nutritional status, smoking history, drinking history, and medical history were assessed. In the analysis, structural equation modeling was used to investigate the association between oral condition and subjective well-being. Confirmatory factor analysis revealed oral condition as a latent variable, including tongue pressure, oral diadochokinesis /pa/, /ta/, /ka/, occlusal force, masticatory ability, subjective swallowing function, and number of functional teeth. Structural Equation Modeling revealed that oral condition was positively correlated with nutritional status, and nutritional status was positively correlated with the World Health Organization-5 Well-Being Index. These findings suggest that oral condition may influence subjective well-being via nutritional status or social environmental factors.

    DOI: 10.1371/journal.pone.0295078

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  • Oral function, nutritional status and physical status in Japanese independent older adults. 国際誌

    Nanami Sawada, Noriko Takeuchi, Daisuke Ekuni, Manabu Morita

    Gerodontology   39 ( 4 )   359 - 365   2022年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES: To clarify the associations among oral status, nutritional status and physical status in Japanese independent older adults. BACKGROUND: It is important to focus on factors affecting physical status associated with life dysfunction, long-term care and mortality. However, there are very few reports of the associations among oral status, nutritional status and physical status. MATERIALS AND METHODS: Patients who visited the Preventive Dentistry Clinic at Okayama University Hospital from November 2017 to January 2019 participated in this cross-sectional study. Number of teeth, periodontal condition and oral function were recorded. Bacteria counts in tongue dorsum, oral wettability, tongue pressure, tongue and lip movement function [oral diadochokinesis (ODK)], masticatory ability, bite force and swallowing function were measured. Nutritional status was assessed by the Mini Nutritional Assessment. Physical frailty status and Elderly Status Assessment Set were also evaluated. These variables were analysed by structural equation modelling (SEM). RESULTS: Data from 203 patients were analysed (63 males and 140 females). Patients ranged in age from 60 to 93 years. The final model of the path diagram was completed by SEM. ODK was positively associated with nutritional status, and nutritional status was negatively associated with frailty. Age was associated with ODK, nutritional status and frailty. CONCLUSION: Based on the associations among age, ODK, nutritional status and frailty, maintaining tongue movement function may contribute to good nutritional status and physical status in Japanese independent older adults.

    DOI: 10.1111/ger.12593

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  • Oral function, nutritional status and physical status in Japanese independent older adults. 国際誌

    Nanami Sawada, Noriko Takeuchi, Daisuke Ekuni, Manabu Morita

    Gerodontology   39 ( 4 )   359 - 365   2022年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES: To clarify the associations among oral status, nutritional status and physical status in Japanese independent older adults. BACKGROUND: It is important to focus on factors affecting physical status associated with life dysfunction, long-term care and mortality. However, there are very few reports of the associations among oral status, nutritional status and physical status. MATERIALS AND METHODS: Patients who visited the Preventive Dentistry Clinic at Okayama University Hospital from November 2017 to January 2019 participated in this cross-sectional study. Number of teeth, periodontal condition and oral function were recorded. Bacteria counts in tongue dorsum, oral wettability, tongue pressure, tongue and lip movement function [oral diadochokinesis (ODK)], masticatory ability, bite force and swallowing function were measured. Nutritional status was assessed by the Mini Nutritional Assessment. Physical frailty status and Elderly Status Assessment Set were also evaluated. These variables were analysed by structural equation modelling (SEM). RESULTS: Data from 203 patients were analysed (63 males and 140 females). Patients ranged in age from 60 to 93 years. The final model of the path diagram was completed by SEM. ODK was positively associated with nutritional status, and nutritional status was negatively associated with frailty. Age was associated with ODK, nutritional status and frailty. CONCLUSION: Based on the associations among age, ODK, nutritional status and frailty, maintaining tongue movement function may contribute to good nutritional status and physical status in Japanese independent older adults.

    DOI: 10.1111/ger.12593

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  • Oral function, nutritional status and physical status in Japanese independent older adults. 国際誌

    Nanami Sawada, Noriko Takeuchi, Daisuke Ekuni, Manabu Morita

    Gerodontology   39 ( 4 )   359 - 365   2022年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES: To clarify the associations among oral status, nutritional status and physical status in Japanese independent older adults. BACKGROUND: It is important to focus on factors affecting physical status associated with life dysfunction, long-term care and mortality. However, there are very few reports of the associations among oral status, nutritional status and physical status. MATERIALS AND METHODS: Patients who visited the Preventive Dentistry Clinic at Okayama University Hospital from November 2017 to January 2019 participated in this cross-sectional study. Number of teeth, periodontal condition and oral function were recorded. Bacteria counts in tongue dorsum, oral wettability, tongue pressure, tongue and lip movement function [oral diadochokinesis (ODK)], masticatory ability, bite force and swallowing function were measured. Nutritional status was assessed by the Mini Nutritional Assessment. Physical frailty status and Elderly Status Assessment Set were also evaluated. These variables were analysed by structural equation modelling (SEM). RESULTS: Data from 203 patients were analysed (63 males and 140 females). Patients ranged in age from 60 to 93 years. The final model of the path diagram was completed by SEM. ODK was positively associated with nutritional status, and nutritional status was negatively associated with frailty. Age was associated with ODK, nutritional status and frailty. CONCLUSION: Based on the associations among age, ODK, nutritional status and frailty, maintaining tongue movement function may contribute to good nutritional status and physical status in Japanese independent older adults.

    DOI: 10.1111/ger.12593

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  • The Impact of Oral Health Behaviors, Health Belief Model, and Absolute Risk Aversion on the Willingness of Japanese University Students to Undergo Regular Dental Check-Ups: A Cross-Sectional Study. 国際誌

    Ichiro Sumita, Naoki Toyama, Daisuke Ekuni, Takayuki Maruyama, Aya Yokoi, Daiki Fukuhara, Yoko Uchida-Fukuhara, Momoko Nakahara, Manabu Morita

    International journal of environmental research and public health   19 ( 21 )   2022年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Oral health behaviors, risk aversion, and the health belief model are associated with health behaviors. However, there have been few studies that investigated the association between these factors and the willingness to undergo regular dental check-ups. The purpose of this cross-sectional study was to investigate the associations between the willingness of Japanese university students to undergo regular dental check-ups and oral health behaviors, the health belief model, and absolute risk aversion. An analysis was conducted with the cooperation of questionnaire respondents (n = 748) who underwent dental check-ups at Okayama University. The students answered questionnaires on oral health behaviors, the health belief model, absolute risk aversion, and willingness to undergo regular dental check-ups. The logistic regression analysis showed significant positive associations (p < 0.05) between oral health behaviors (use of the inter-dental brush and the dental floss) and the health belief model with the willingness to undergo regular dental check-ups. However, there was no significant association with absolute risk aversion (p > 0.05). These results suggest that willingness to undergo regular dental check-ups was associated with oral health behaviors and the health belief model, but not with absolute risk aversion.

    DOI: 10.3390/ijerph192113920

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  • Trends in Self-Rated Oral Health and Its Associations with Oral Health Status and Oral Health Behaviors in Japanese University Students: A Cross-Sectional Study from 2011 to 2019. 国際誌

    Momoko Nakahara, Naoki Toyama, Daisuke Ekuni, Noriko Takeuchi, Takayuki Maruyama, Aya Yokoi, Daiki Fukuhara, Nanami Sawada, Yukiho Nakashima, Manabu Morita

    International journal of environmental research and public health   19 ( 20 )   2022年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Self-rated oral health (SROH) is a valid, comprehensive indicator of oral health status. The purpose of this cross-sectional study was to analyze how oral health behaviors and clinical oral status were associated with SROH and how they had changed over the course of nine years in Japanese university students. Data were obtained from 17,996 students who underwent oral examinations and completed self-questionnaires from 2011 to 2019. Oral status was assessed using the decayed and filled teeth scores, bleeding on probing (BOP), probing pocket depth, the Oral Hygiene Index-Simplified (OHI-S), oral health behaviors, and related factors. SROH improved from 2011 to 2019. The logistic regression model showed that university students who were female and had a high daily frequency of tooth brushing, no BOP, no decayed teeth, no filled teeth, and a low OHI-S score and were significantly more likely to report very good, good, or fair SROH. An interaction effect was observed between survey year and regular dental check-ups (year × regular dental check-ups). The improvement trend in SROH might be associated with changes in oral health behaviors and oral health status.

    DOI: 10.3390/ijerph192013580

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  • 地域在住高齢者における主観的認知機能低下と口腔機能およびソーシャル・キャピタルとの関連

    竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

    老年歯科医学   37 ( 2 )   64 - 75   2022年9月

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    記述言語:日本語   出版者・発行元:(一社)日本老年歯科医学会  

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  • 地域在住高齢者における主観的認知機能低下と口腔機能およびソーシャル・キャピタルとの関連

    竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

    老年歯科医学   37 ( 2 )   64 - 75   2022年9月

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    記述言語:日本語   出版者・発行元:(一社)日本老年歯科医学会  

    わが国では認知症高齢者が急増しているが,認知症に対する有効な治療法はまだ確立されていない。そのため,認知症の発症を予防する手段を模索することが望まれる。本研究の目的は,地域在住高齢者を対象に口腔機能およびソーシャル・キャピタル(SC)と認知機能低下の関係を調査することである。2018年5月~8月に,農村地域在住の高齢者を対象に,世帯,学歴,基本チェックリスト,農村SC,舌圧,オーラルディアドコキネシス(ODK),現在歯数,主観的口腔機能を調査した。認知機能は基本チェックリストの項目より評価した。主観的認知機能低下を従属変数とした二項ロジスティック回帰分析を行った。また,主観的認知機能の低下に与える要因間の関係を検討するために共分散構造分析を行った。分析対象者は73人(男性24人,女性49人,平均年齢80.0±10.6歳)であった。二項ロジスティック回帰分析の結果,主観的認知機能低下と有意な関連がみられたのは,うつ病の可能性(オッズ比6.392,95%信頼区間1.208~33.821),ODK/ta/(オッズ比0.663,95%信頼区間0.457~0.962),農村SC(オッズ比0.927,95%信頼区間0.859~0.999)であった。共分散構造分析の結果,「年齢が高いほどODK/ta/値が低く,うつ病の可能性がある」「ODK/ta/値が高く,うつ病の可能性がなく,農村SC値が高いほど,主観的認知機能低下がない」という関係がみられた。結論として,農村地域の高齢者を対象に主観的認知機能低下に関連する要因を調査した結果,SC,うつ病の可能性および舌の巧緻性が関連していた。(著者抄録)

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    その他リンク: https://search.jamas.or.jp/default/link?pub_year=2022&ichushi_jid=J02171&link_issn=&doc_id=20221028310001&doc_link_id=10.11259%2Fjsg.37.2_64&url=https%3A%2F%2Fdoi.org%2F10.11259%2Fjsg.37.2_64&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_2.gif

  • Relationship between Psychological Stress Determined by Voice Analysis and Periodontal Status: A Cohort Study. 国際誌

    Takayuki Maruyama, Daisuke Ekuni, Masakazu Higuchi, Eiji Takayama, Shinichi Tokuno, Manabu Morita

    International journal of environmental research and public health   19 ( 15 )   2022年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In modern society, evaluation and management of psychological stress may be important for the prevention of periodontal disease. The purpose of this study was to examine the relationship between psychological stress (vitality and mental activity) evaluated by Mind Monitoring System (MIMOSYS) and periodontal status. Forty students of Okayama University underwent the oral examination and self-reported questionnaire on the first day (baseline) and the 14th day (follow-up). Voice recording was performed every day with the MIMOSYS app during the whole study period. The participants completed the Patient Health Questionnaire (PHQ)-9 and Beck Depression Inventory (BDI) at baseline and at follow-up. Spearman's rank correlation coefficient was used to determine the significance of correlations among variables. The PHQ-9 and BDI scores were negatively correlated with vitality in the morning. Change in vitality in the morning was significantly correlated with changes in periodontal inflammation. Mental activity was significantly correlated with change in mean probing pocket depth. This result shows that measurement of psychological stress using a voice-based tool to assess mental health may contribute to the early detection of periodontal disease.

    DOI: 10.3390/ijerph19159489

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  • Association between serum miRNAs and gingival gene expression in an obese rat model. 国際誌

    Takayuki Maruyama, Terumasa Kobayashi, Yoshio Sugiura, Toshiki Yoneda, Daisuke Ekuni, Manabu Morita

    Journal of periodontal research   57 ( 3 )   502 - 509   2022年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    INTRODUCTION: Recent studies have reported a relationship between periodontitis and obesity; however, the mechanisms of obesity's effects on periodontitis are not well understood. On the other hand, microRNAs (miRNAs) are known to play key roles in the post-transcriptional regulation gene expression by suppressing translation and protein synthesis. We examined the association between obesity-related miRNAs and gene expression in gingival tissue using miRNA-messenger RNA (mRNA) pairing analysis in an obese rat model. METHODS: Sixteen male Wistar rats aged 8 weeks old were divided into two groups: the control group was fed a normal powdered food for 8 weeks, and the obesity group was fed a high-fat diet for 8 weeks. Distance from the cement-enamel junction to the alveolar bone crest of the first molars was measured. miRNA microarray analysis was performed on samples of serum and gingival tissue; the resulting data were used to calculate fold changes in miRNA levels in the obesity group relative to the control group, and miRNA-mRNA pairing analysis was performed to identify mRNAs potentially targeted by miRNAs of interest. RESULTS: Alveolar bone loss in the obesity group exceeded that in the control group (p = .017). miRNA-mRNA pairing analysis identified an association between 4 miRNAs (miR-759, miR-9a-3p, miR-203b-3p, and miR-878) that were differentially expressed in the obesity and control groups and 7 genes (Ly86, Arid5b, Rgs18, Mlana, P2ry13, Kif1b, and Myt1) expressed in gingival tissue. CONCLUSION: This study revealed that several miRNAs play an important role in the mechanism of periodontal disease progression induced by the obesity.

    DOI: 10.1111/jre.12979

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  • Maternal Gut Microbiome Decelerates Fetal Endochondral Bone Formation by Inducing Inflammatory Reaction. 国際誌

    Yoko Uchida-Fukuhara, Takako Hattori, Shanqi Fu, Sei Kondo, Miho Kuwahara, Daiki Fukuhara, Md Monirul Islam, Kota Kataoka, Daisuke Ekuni, Satoshi Kubota, Manabu Morita, Mika Iikegame, Hirohiko Okamura

    Microorganisms   10 ( 5 )   2022年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To investigate the effect of the maternal gut microbiome on fetal endochondral bone formation, fetuses at embryonic day 18 were obtained from germ-free (GF) and specific-pathogen-free (SPF) pregnant mothers. Skeletal preparation of the fetuses' whole bodies did not show significant morphological alterations; however, micro-CT analysis of the tibiae showed a lower bone volume fraction in the SPF tibia. Primary cultured chondrocytes from fetal SPF rib cages showed a lower cell proliferation and lower accumulation of the extracellular matrix. RNA-sequencing analysis showed the induction of inflammation-associated genes such as the interleukin (IL) 17 receptor, IL 6, and immune-response genes in SPF chondrocytes. These data indicate that the maternal gut microbiome in SPF mice affects fetal embryonic endochondral ossification, possibly by changing the expression of genes related to inflammation and the immune response in fetal cartilage. The gut microbiome may modify endochondral ossification in the fetal chondrocytes passing through the placenta.

    DOI: 10.3390/microorganisms10051000

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  • New Method of Avoiding Underestimation of Caries Incidence and Its Association with Possible Risk Factors in Japanese University Students: A Prospective Cohort Study. 国際誌

    Daisuke Ekuni, Naoki Toyama, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   19 ( 4 )   2022年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The objective of this three-year prospective cohort study was to investigate the association between a new definition of an increase in dental caries and risk factors in Japanese young adults. Data of Okayama University students who volunteered to undergo oral examinations and answer questionnaires in 2015 and 2018 were analyzed. The status of filled teeth and the status of occlusal/proximal surfaces of filled or decayed teeth were recorded. An increase in dental caries was defined as a change in the status of filled teeth and/or an increase in dental caries of occlusal and proximal surfaces. A total of 393 participants (18.2 ± 0.8 years) were analyzed. First and second molars showed a high prevalence of dental caries. Of the participants, 144 (36.6%) showed an increase in dental caries. In all the participants and in the females, the decayed, missing, and filled teeth (DMFT) score and history of orthodontic treatment at baseline were significantly associated with an increase in dental caries (p < 0.05) in logistic regression analyses. In the males, the DMFT score and the daily frequency of snacking (≥2) at baseline were significantly associated with an increase in dental caries (p = 0.04). The DMFT score and history of orthodontic treatment at baseline can be risk factors for an increase in dental caries using the new definition in young adults.

    DOI: 10.3390/ijerph19042490

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  • Oral Factors as Predictors of Frailty in Community-Dwelling Older People: A Prospective Cohort Study. 国際誌

    Noriko Takeuchi, Nanami Sawada, Daisuke Ekuni, Manabu Morita

    International journal of environmental research and public health   19 ( 3 )   2022年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purpose of this prospective cohort study was to identify predictors for frailty among possible oral factors in community-dwelling older people. Ninety-seven participants (≥60 years old) without frailty at baseline were included and assigned to either the robust or the frailty group after 2-year follow-up. The frailty was defined using the Japan Cardiovascular Health Study index. The numbers of present and functional teeth and periodontal disease severity were recorded. Bacterial counts on the dorsum of the tongue, oral moisture, tongue pressure, occlusal force, masticatory ability, and the oral diadochokinesis (ODK) rate were measured. Swallowing function, along with psychosocial status, relationships with communities and people, nutritional status, medical history, and comorbidities were evaluated using a questionnaire. The newly identified frailty group at follow-up showed significantly lower values in the number of teeth present, ODK/ta/sound and ODK/ka/sound rates, and clinical attachment level at baseline compared to the robust group. A logistic regression model showed a significantly negative association between the ODK/ta/sound rate at baseline and the incidence of frailty. Articulatory oral motor skill was found to be a predictor of frailty after two years.

    DOI: 10.3390/ijerph19031145

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  • 岡山大学新入生の口腔健康状態および主観的口腔健康評価の9年間の変化

    中原 桃子, 外山 直樹, 江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, イスラム・モハマド・モニルル, 澤田 ななみ, 中島 千穂, 出口 純也, 森田 学

    口腔衛生学会雑誌   72 ( 1 )   54 - 54   2022年1月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 家庭での毎日の受動喫煙曝露は大学生の歯周病と関係する(横断研究)

    江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, 外山 直樹, イスラム・モハマド・モニルル, 澤田 ななみ, 中原 桃子, 中島 千穂, 出口 純也, 福原 瑶子, 森田 学

    口腔衛生学会雑誌   72 ( 1 )   54 - 54   2022年1月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • Features of the oral microbiome in Japanese elderly people with 20 or more teeth and a non-severe periodontal condition during periodontal maintenance treatment: A cross-sectional study. 国際誌

    Naoki Toyama, Daisuke Ekuni, Aya Yokoi, Daiki Fukuhara, Md Monirul Islam, Nanami Sawada, Yukiho Nakashima, Momoko Nakahara, Ichiro Sumita, Manabu Morita

    Frontiers in cellular and infection microbiology   12   957890 - 957890   2022年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    INTRODUCTION: The aim of the present study was to characterize the profile and diversity of the oral microbiome of a periodontally non-severe group with ≥20 teeth in comparison with a severe periodontitis group of elderly Japanese people. METHODS: A total of 50 patients who had ≥20 teeth and aged ≥60 years were recruited, and 34 participants (13 non-severe participants) were analyzed. After oral rinse (saliva after rinsing) sample collection, the V3-V4 regions of the 16S rRNA gene were sequenced to investigate microbiome composition, alpha diversity (Shannon index, Simpson index, richness, and evenness), and beta diversity using principal coordinate analysis (PCoA) based on weighted and unweighted UniFrac distances. A linear discriminant analysis effect size was calculated to identify bacterial species in the periodontally non-severe group. RESULTS: The periodontally non-severe group showed lower alpha diversity than that of the severe periodontitis group (p &lt;0.05); however, the beta diversities were not significantly different. A higher relative abundance of four bacterial species (Prevotella nanceiensis, Gemella sanguinis, Fusobacterium periodonticum, and Haemophilus parainfluenzae) was observed in the non-severe group than that in the severe periodontitis group. CONCLUSION: The oral microbiome in elderly Japanese people with ≥20 teeth and a non-severe periodontal condition was characterized by low alpha diversity and the presence of four bacterial species.

    DOI: 10.3389/fcimb.2022.957890

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  • Association between Self-Reported Chewing Status and Glycemic Control in Japanese Adults. 国際誌

    Komei Iwai, Tetsuji Azuma, Takatoshi Yonenaga, Daisuke Ekuni, Kazutoshi Watanabe, Akihiro Obora, Fumiko Deguchi, Takao Kojima, Manabu Morita, Takaaki Tomofuji

    International journal of environmental research and public health   18 ( 18 )   2021年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This cross-sectional study investigated the relationship between self-reported chewing status and glycemic control in 30,938 Japanese adults who participated in health checkups. Chewing status was evaluated using a self-reported questionnaire. We defined high hemoglobin A1c (HbA1c) levels as a HbA1c level ≥6.5%; 692 (2.2%) respondents met this criterion. After adjusting for gender, age, smoking status, exercise habits, body mass index and eating speed, high HbA1c levels was found to be associated with male gender (odds ratio (OR), 1.568; 95% confidence interval (CI), 1.310 to 1.878; p < 0.001), older age (OR, 1.077; 95% CI, 1.068 to 1.087; p < 0.001), higher body mass index (OR, 1.246; 95% CI, 1.225 to 1.268; p < 0.001), current smoker status (OR, 1.566; 95% CI, 1.303 to 1.882; p < 0.001) and chewing difficulty (OR, 1.302; 95% CI, 1.065 to 1.591; p < 0.05). In conclusion, self-reported chewing difficulty was associated with high HbA1c levels in Japanese adults.

    DOI: 10.3390/ijerph18189548

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  • Detection of Salivary miRNAs That Predict Chronic Periodontitis Progression: A Cohort Study. 国際誌

    Kohei Fujimori, Toshiki Yoneda, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Yoshio Sugiura, Manabu Morita

    International journal of environmental research and public health   18 ( 15 )   2021年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The aim of this two-year cohort study was to investigate salivary microRNAs (miRNAs) that predict periodontitis progression. A total of 120 patients who underwent supportive periodontal therapy were recruited. Unstimulated whole saliva was collected at baseline. Two years later, 44 patients were followed up (median age, 67.1 years) and divided into two groups: progression group (n = 22), with one or more sites with clinical attachment level (CAL) progression (>3 mm compared with baseline) or tooth extraction due to periodontitis progression; and the control group (n = 22), which did not exhibit CAL progression. In the microarray analysis of salivary miRNAs, hsa-miR-5571-5p, hsa-miR-17-3p, hsa-let-7f-5p, hsa-miR-4724-3p, hsa-miR-99a-5p, hsa-miR-200a-3p, hsa-miR-28-5p, hsa-miR-320d, and hsa-miR-31-5p showed fold change values <0.5 or ≥2.0 in the progression group compared with the control group (p < 0.05). On receiver operating characteristic curve analysis, areas under the curves of hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were >0.7, indicating fair discrimination power. The expressions of salivary hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were associated with periodontitis progression in patients with chronic periodontitis. These salivary miRNAs may be new biomarkers for progression of periodontitis, and monitoring them may contribute to new diagnostics and precision medicine for periodontitis.

    DOI: 10.3390/ijerph18158010

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  • Comprehensive Analysis of Risk Factors for Periodontitis Focusing on the Saliva Microbiome and Polymorphism. 国際誌

    Naoki Toyama, Daisuke Ekuni, Daisuke Matsui, Teruhide Koyama, Masahiro Nakatochi, Yukihide Momozawa, Michiaki Kubo, Manabu Morita

    International journal of environmental research and public health   18 ( 12 )   2021年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Few studies have exhaustively assessed relationships among polymorphisms, the microbiome, and periodontitis. The objective of the present study was to assess associations simultaneously among polymorphisms, the microbiome, and periodontitis. We used propensity score matching with a 1:1 ratio to select subjects, and then 22 individuals (mean age ± standard deviation, 60.7 ± 9.9 years) were analyzed. After saliva collection, V3-4 regions of the 16S rRNA gene were sequenced to investigate microbiome composition, alpha diversity (Shannon index, Simpson index, Chao1, and abundance-based coverage estimator) and beta diversity using principal coordinate analysis (PCoA) based on weighted and unweighted UniFrac distances. A total of 51 single-nucleotide polymorphisms (SNPs) related to periodontitis were identified. The frequencies of SNPs were collected from Genome-Wide Association Study data. The PCoA of unweighted UniFrac distance showed a significant difference between periodontitis and control groups (p < 0.05). There were no significant differences in alpha diversity and PCoA of weighted UniFrac distance (p > 0.05). Two families (Lactobacillaceae and Desulfobulbaceae) and one species (Porphyromonas gingivalis) were observed only in the periodontitis group. No SNPs showed significant expression. These results suggest that periodontitis was related to the presence of P. gingivalis and the families Lactobacillaceae and Desulfobulbaceae but not SNPs.

    DOI: 10.3390/ijerph18126430

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  • Parafunctional masseter muscle activity during waking is related to periodontitis progression: A pilot prospective cohort study. 査読 国際誌

    Daisuke Ekuni, Seiya Kato, Shigehisa Kawakami, Takayuki Maruyama, Kota Kataoka, Yoko Uchida-Fukuhara, Daiki Fukuhara, Naoki Toyama, Aya Yokoi, Md Monirul Islam, Subrina Binta Khair, Naoki Kodama, Manabu Morita

    Journal of clinical periodontology   48 ( 6 )   785 - 794   2021年6月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    AIM: The purpose of this pilot prospective cohort study was to investigate the effects of parafunctional masseter muscle activity on periodontitis progression among patients receiving supporting periodontal therapy (SPT). MATERIALS AND METHODS: We collected data of patients treated at Okayama University Hospital from August 2014 to September 2018. The progression group was defined as patients with ≥2 teeth demonstrating a longitudinal loss of proximal attachment of ≥3 mm during the 3-year study period and/or at least one tooth extraction due to periodontitis progression. Surface electromyography of masseter muscles at baseline was continuously recorded while patients were awake and asleep. RESULTS: We analysed 48 patients (36 females) aged 66.8 ± 9.1 years (mean ± SD). The rate of parafunctional masseter muscle activity during waking hours and sleeping hours at baseline was 60.4% and 52.1%, respectively. Cox's proportional hazards regression model showed that the incidence of periodontitis progression was significantly associated with number of teeth present (p = 0.001) and parafunctional masseter muscle activity during waking hours (p = 0.041). CONCLUSION: Our results suggest that parafunctional masseter muscle activity during waking hours is a risk factor for periodontitis progression among patients receiving SPT.

    DOI: 10.1111/jcpe.13432

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jcpe.13432

  • Parafunctional masseter muscle activity during waking is related to periodontitis progression: A pilot prospective cohort study. 国際誌

    Daisuke Ekuni, Seiya Kato, Shigehisa Kawakami, Takayuki Maruyama, Kota Kataoka, Yoko Uchida-Fukuhara, Daiki Fukuhara, Naoki Toyama, Aya Yokoi, Md Monirul Islam, Subrina Binta Khair, Naoki Kodama, Manabu Morita

    Journal of clinical periodontology   48 ( 6 )   785 - 794   2021年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    AIM: The purpose of this pilot prospective cohort study was to investigate the effects of parafunctional masseter muscle activity on periodontitis progression among patients receiving supporting periodontal therapy (SPT). MATERIALS AND METHODS: We collected data of patients treated at Okayama University Hospital from August 2014 to September 2018. The progression group was defined as patients with ≥2 teeth demonstrating a longitudinal loss of proximal attachment of ≥3 mm during the 3-year study period and/or at least one tooth extraction due to periodontitis progression. Surface electromyography of masseter muscles at baseline was continuously recorded while patients were awake and asleep. RESULTS: We analysed 48 patients (36 females) aged 66.8 ± 9.1 years (mean ± SD). The rate of parafunctional masseter muscle activity during waking hours and sleeping hours at baseline was 60.4% and 52.1%, respectively. Cox's proportional hazards regression model showed that the incidence of periodontitis progression was significantly associated with number of teeth present (p = 0.001) and parafunctional masseter muscle activity during waking hours (p = 0.041). CONCLUSION: Our results suggest that parafunctional masseter muscle activity during waking hours is a risk factor for periodontitis progression among patients receiving SPT.

    DOI: 10.1111/jcpe.13432

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  • Parafunctional masseter muscle activity during waking is related to periodontitis progression: A pilot prospective cohort study. 国際誌

    Daisuke Ekuni, Seiya Kato, Shigehisa Kawakami, Takayuki Maruyama, Kota Kataoka, Yoko Uchida-Fukuhara, Daiki Fukuhara, Naoki Toyama, Aya Yokoi, Md Monirul Islam, Subrina Binta Khair, Naoki Kodama, Manabu Morita

    Journal of clinical periodontology   48 ( 6 )   785 - 794   2021年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    AIM: The purpose of this pilot prospective cohort study was to investigate the effects of parafunctional masseter muscle activity on periodontitis progression among patients receiving supporting periodontal therapy (SPT). MATERIALS AND METHODS: We collected data of patients treated at Okayama University Hospital from August 2014 to September 2018. The progression group was defined as patients with ≥2 teeth demonstrating a longitudinal loss of proximal attachment of ≥3 mm during the 3-year study period and/or at least one tooth extraction due to periodontitis progression. Surface electromyography of masseter muscles at baseline was continuously recorded while patients were awake and asleep. RESULTS: We analysed 48 patients (36 females) aged 66.8 ± 9.1 years (mean ± SD). The rate of parafunctional masseter muscle activity during waking hours and sleeping hours at baseline was 60.4% and 52.1%, respectively. Cox's proportional hazards regression model showed that the incidence of periodontitis progression was significantly associated with number of teeth present (p = 0.001) and parafunctional masseter muscle activity during waking hours (p = 0.041). CONCLUSION: Our results suggest that parafunctional masseter muscle activity during waking hours is a risk factor for periodontitis progression among patients receiving SPT.

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  • 「口臭による日常生活への支障」に関連する要因

    佐保 輝, 横井 彩, 丸山 貴之, 竹内 倫子, 江國 大輔, 森田 学

    日本口臭学会会誌   12 ( 1 )   11 - 17   2021年5月

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    記述言語:日本語   出版者・発行元:日本口臭学会  

    本調査の目的は、自己記入式質問票の「社会生活への支障」「家庭生活への支障」「行動制限」「自身の口臭が原因による他人の嫌がる態度やジェスチャー」の4項目を「口臭による日常生活への支障」と定義しその実態を把握すること、および、「口臭による日常生活への支障」と関連する要因について検討することである。2018年1月から2020年1月までの間に岡山大学病院口臭専門外来を受診した全患者32名(男性12名、女性20名、平均年齢53.2±16.2歳)を対象とした。オーラルクロマ(NISSHAエフアイエス株式会社、大阪)を用い、口腔内の揮発性硫黄化合物の濃度を測定した。自己記入式の質問票を用いて、上記4項目のほかに年齢、性別、口臭を意識するようになったきっかけ、口臭を意識した時期、頻回の歯磨き・うがい薬の使用・舌磨きによる口臭対策、医療者への相談経験を調査した。なお、口臭による日常生活への支障については、「社会生活への支障」「家庭生活への支障」「行動制限」「自身の口臭が原因による他人の嫌がる態度やジェスチャー」の各質問に対する回答【はい(1点)/いいえ(0点)】をもとに、合計スコア0〜4点で評価した。各質問項目との関連についてSpearmanの順位相関係数とカイ二重検定を用いて検討した。有意水準は5%とした。その結果、本調査で定義した「口臭による日常生活への支障」を有する患者が87.5%存在した。その支障の程度が大きい者は、長期にわたって口臭を意識しており、医療者への相談や複数の口臭対策を行っていた。一方で、実際の口腔内の揮発性硫黄化合物の濃度は有意に低いことが明らかになった。本調査結果は、口臭専門外来における新たな視点を提供する可能性がある。(著者抄録)

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  • 若年者と高齢者との間での口腔機能の比較

    澤田 ななみ, 竹内 倫子, 江國 大輔, 森田 学

    老年歯科医学   35 ( 4 )   363 - 364   2021年3月

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    記述言語:日本語   出版者・発行元:(一社)日本老年歯科医学会  

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  • 基本チェックリストの口腔機能3項目と口腔機能との関連

    竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

    老年歯科医学   35 ( 4 )   317 - 318   2021年3月

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    記述言語:日本語   出版者・発行元:(一社)日本老年歯科医学会  

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  • Association between the incidence of early childhood caries and attending childcare among toddlers in a rural area of Japan: a prospective cohort study. 国際誌

    Aya Yokoi, Noriko Takeuchi, Daisuke Ekuni, Manabu Morita

    Acta odontologica Scandinavica   79 ( 2 )   118 - 123   2021年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: The purpose of this study was to investigate the association between the incidence of early childhood caries (ECC) and factors of the daytime childcare environment among toddlers in a rural area of Japan. MATERIAL AND METHODS: This was a prospective cohort study. Data collected from 2011 to 2015 were obtained for initially caries-free, 18-month-old toddlers (N = 640). Dentists recorded the numbers of decayed, missing and filled teeth (caries incidence) of toddlers at baseline (age 18 months) and follow-up (age 3 years). In addition, a questionnaire survey was conducted on guardians at baseline to record data on lifestyle. RESULTS: In total, 159 (24.8%) toddlers had newly identified ECC at follow-up. Logistic regression analyses indicated that the incidence of ECC was significantly associated with receiving daytime care at a nursery school (odds ratio [OR], 1.55; 95% confidence interval [CI], 1.05-2.30; p = .029), prolonged breastfeeding (OR, 1.71; 95%CI, 1.15-2.55; p = .008), and snacking ≥3 times a day (OR, 2.39; 95%CI, 1.53-3.74; p < .001). CONCLUSION: These findings suggest that receiving daytime care at a nursery school, prolonged breastfeeding, and frequent snacking are associated with an increased risk of ECC among Japanese toddlers in rural areas.

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  • Oral diadochokinesis is related to decline in swallowing function among community-dwelling Japanese elderly: a cross-sectional study. 国際誌

    Noriko Takeuchi, Nanami Sawada, Daisuke Ekuni, Manabu Morita

    Aging clinical and experimental research   33 ( 2 )   399 - 405   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Since oral organs function in the first stage of the swallowing process, it is possible that decline in swallowing function can be partly related to oral frailty. AIMS: The purpose of this study was to investigate the association between swallowing function and oral function among community-dwelling elderly Japanese and to propose cut-off values for screening of decline in swallowing function. METHODS: A total of 188 participants were included in the analysis. The number of present teeth and functioning teeth were counted. Bacteria counts in tongue coat, oral wettability, tongue pressure, oral diadochokinesis (ODK), masticatory ability and bite force were examined. Swallowing function was assessed using the 10-item Eating Assessment Tool (EAT-10). Receiver operating characteristic curves were used to determine the cut-off points of each oral function parameter. A logistic regression model was performed to determine the combination of parameters with the highest accuracy for differentiating decline in swallowing function from normal. RESULTS: Subjects with ODK /pa/ sound < 6.2 times/second had higher prevalence of swallowing problems than those with 6.2 times/second or more. EAT-10 scores of one or more were significantly related to older age (≥ 71 years old; odds ratio [OR] 4.321) and reduced ODK /pa/ sound (< 6.2 times/second; OR 2.914). CONCLUSIONS: Among community-dwelling elderly Japanese, those who were suspected of having decline in swallowing function had lower oral function than those who did not, and the cut-off values were 71 years of age and ODK /pa/ sound 6.2 times/s.

    DOI: 10.1007/s40520-020-01547-7

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  • Living with Family Is Directly Associated with Regular Dental Checkup and Indirectly Associated with Gingival Status among Japanese University Students: A 3-Year Cohort Study. 査読 国際誌

    Momoko Nakahara, Daisuke Ekuni, Kota Kataoka, Aya Yokoi, Yoko Uchida-Fukuhara, Daiki Fukuhara, Terumasa Kobayashi, Naoki Toyama, Hikari Saho, Md Monirul Islam, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   18 ( 1 )   324 - 324   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    Although some studies showed that lifestyle was associated with oral health behavior, few studies investigated the association between household type and oral health behavior. The aim of this prospective cohort study was to investigate the association between household type, oral health behavior, and periodontal status among Japanese university students. Data were obtained from 377 students who received oral examinations and self-questionnaires in 2016 and 2019. We assessed periodontal status using the percentage of bleeding on probing (%BOP), probing pocket depth, oral hygiene status, oral health behaviors, and related factors. We used structural equation modeling to determine the association between household type, oral health behaviors, gingivitis, and periodontitis. At follow-up, 252 students did not live with their families. The mean ± standard deviation of %BOP was 35.5 ± 24.7 at baseline and 32.1 ± 25.3 at follow-up. In the final model, students living with their families were significantly more likely to receive regular dental checkup than those living alone. Regular checkup affected the decrease in calculus. The decrease in calculus affected the decrease in %BOP over 3 years. Living with family was directly associated with regular dental checkups and indirectly contributed to gingival status among Japanese university students.

    DOI: 10.3390/ijerph18010324

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  • Living with Family Is Directly Associated with Regular Dental Checkup and Indirectly Associated with Gingival Status among Japanese University Students: A 3-Year Cohort Study. 国際誌

    Momoko Nakahara, Daisuke Ekuni, Kota Kataoka, Aya Yokoi, Yoko Uchida-Fukuhara, Daiki Fukuhara, Terumasa Kobayashi, Naoki Toyama, Hikari Saho, Md Monirul Islam, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   18 ( 1 )   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Although some studies showed that lifestyle was associated with oral health behavior, few studies investigated the association between household type and oral health behavior. The aim of this prospective cohort study was to investigate the association between household type, oral health behavior, and periodontal status among Japanese university students. Data were obtained from 377 students who received oral examinations and self-questionnaires in 2016 and 2019. We assessed periodontal status using the percentage of bleeding on probing (%BOP), probing pocket depth, oral hygiene status, oral health behaviors, and related factors. We used structural equation modeling to determine the association between household type, oral health behaviors, gingivitis, and periodontitis. At follow-up, 252 students did not live with their families. The mean ± standard deviation of %BOP was 35.5 ± 24.7 at baseline and 32.1 ± 25.3 at follow-up. In the final model, students living with their families were significantly more likely to receive regular dental checkup than those living alone. Regular checkup affected the decrease in calculus. The decrease in calculus affected the decrease in %BOP over 3 years. Living with family was directly associated with regular dental checkups and indirectly contributed to gingival status among Japanese university students.

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  • Living with Family Is Directly Associated with Regular Dental Checkup and Indirectly Associated with Gingival Status among Japanese University Students: A 3-Year Cohort Study. 国際誌

    Momoko Nakahara, Daisuke Ekuni, Kota Kataoka, Aya Yokoi, Yoko Uchida-Fukuhara, Daiki Fukuhara, Terumasa Kobayashi, Naoki Toyama, Hikari Saho, Md Monirul Islam, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   18 ( 1 )   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Although some studies showed that lifestyle was associated with oral health behavior, few studies investigated the association between household type and oral health behavior. The aim of this prospective cohort study was to investigate the association between household type, oral health behavior, and periodontal status among Japanese university students. Data were obtained from 377 students who received oral examinations and self-questionnaires in 2016 and 2019. We assessed periodontal status using the percentage of bleeding on probing (%BOP), probing pocket depth, oral hygiene status, oral health behaviors, and related factors. We used structural equation modeling to determine the association between household type, oral health behaviors, gingivitis, and periodontitis. At follow-up, 252 students did not live with their families. The mean ± standard deviation of %BOP was 35.5 ± 24.7 at baseline and 32.1 ± 25.3 at follow-up. In the final model, students living with their families were significantly more likely to receive regular dental checkup than those living alone. Regular checkup affected the decrease in calculus. The decrease in calculus affected the decrease in %BOP over 3 years. Living with family was directly associated with regular dental checkups and indirectly contributed to gingival status among Japanese university students.

    DOI: 10.3390/ijerph18010324

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  • Association between Household Exposure to Secondhand Smoke and Dental Caries among Japanese Young Adults: A Cross-Sectional Study. 査読 国際誌

    Hikari Saho, Ayano Taniguchi-Tabata, Daisuke Ekuni, Aya Yokoi, Kouta Kataoka, Daiki Fukuhara, Naoki Toyama, Md Monirul Islam, Nanami Sawada, Yukiho Nakashima, Momoko Nakahara, Junya Deguchi, Yoko Uchida-Fukuhara, Toshiki Yoneda, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   17 ( 22 )   8623 - 8623   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    The long-term effects of secondhand smoke (SHS) on dental caries among Japanese young adults remain unclear. The purpose of this cross-sectional study was to evaluate whether household exposure to SHS is associated with dental caries in permanent dentition among Japanese young adults. The study sample included 1905 first-year university students (age range: 18–19 years) who answered a questionnaire and participated in oral examinations. The degree of household exposure to SHS was categorized into four levels according to the SHS duration: no experience (−), past, current SHS &lt; 10 years, and current SHS ≥ 10 years. Dental caries are expressed as the total number of decayed, missing, and filled teeth (DMFT) score. The relationships between SHS and dental caries were determined by logistic regression analysis. DMFT scores (median (25th percentile, 75th percentile)) were significantly higher in the current SHS ≥ 10 years (median: 1.0 (0.0, 3.0)) than in the SHS—(median: 0.0 (0.0, 2.0)); p = 0.001). DMFT ≥ 1 was significantly associated with SHS ≥ 10 years (adjusted odds ratio: 1.50, 95% confidence interval: 1.20–1.87, p &lt; 0.001). Long-term exposure to SHS (≥10 years) was associated with dental caries in permanent dentition among Japanese young adults.

    DOI: 10.3390/ijerph17228623

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  • Association between Household Exposure to Secondhand Smoke and Dental Caries among Japanese Young Adults: A Cross-Sectional Study. 国際誌

    Hikari Saho, Ayano Taniguchi-Tabata, Daisuke Ekuni, Aya Yokoi, Kouta Kataoka, Daiki Fukuhara, Naoki Toyama, Md Monirul Islam, Nanami Sawada, Yukiho Nakashima, Momoko Nakahara, Junya Deguchi, Yoko Uchida-Fukuhara, Toshiki Yoneda, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   17 ( 22 )   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The long-term effects of secondhand smoke (SHS) on dental caries among Japanese young adults remain unclear. The purpose of this cross-sectional study was to evaluate whether household exposure to SHS is associated with dental caries in permanent dentition among Japanese young adults. The study sample included 1905 first-year university students (age range: 18-19 years) who answered a questionnaire and participated in oral examinations. The degree of household exposure to SHS was categorized into four levels according to the SHS duration: no experience (-), past, current SHS < 10 years, and current SHS ≥ 10 years. Dental caries are expressed as the total number of decayed, missing, and filled teeth (DMFT) score. The relationships between SHS and dental caries were determined by logistic regression analysis. DMFT scores (median (25th percentile, 75th percentile)) were significantly higher in the current SHS ≥ 10 years (median: 1.0 (0.0, 3.0)) than in the SHS-(median: 0.0 (0.0, 2.0)); p = 0.001). DMFT ≥ 1 was significantly associated with SHS ≥ 10 years (adjusted odds ratio: 1.50, 95% confidence interval: 1.20-1.87, p < 0.001). Long-term exposure to SHS (≥10 years) was associated with dental caries in permanent dentition among Japanese young adults.

    DOI: 10.3390/ijerph17228623

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  • Association between Household Exposure to Secondhand Smoke and Dental Caries among Japanese Young Adults: A Cross-Sectional Study. 国際誌

    Hikari Saho, Ayano Taniguchi-Tabata, Daisuke Ekuni, Aya Yokoi, Kouta Kataoka, Daiki Fukuhara, Naoki Toyama, Md Monirul Islam, Nanami Sawada, Yukiho Nakashima, Momoko Nakahara, Junya Deguchi, Yoko Uchida-Fukuhara, Toshiki Yoneda, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   17 ( 22 )   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The long-term effects of secondhand smoke (SHS) on dental caries among Japanese young adults remain unclear. The purpose of this cross-sectional study was to evaluate whether household exposure to SHS is associated with dental caries in permanent dentition among Japanese young adults. The study sample included 1905 first-year university students (age range: 18-19 years) who answered a questionnaire and participated in oral examinations. The degree of household exposure to SHS was categorized into four levels according to the SHS duration: no experience (-), past, current SHS < 10 years, and current SHS ≥ 10 years. Dental caries are expressed as the total number of decayed, missing, and filled teeth (DMFT) score. The relationships between SHS and dental caries were determined by logistic regression analysis. DMFT scores (median (25th percentile, 75th percentile)) were significantly higher in the current SHS ≥ 10 years (median: 1.0 (0.0, 3.0)) than in the SHS-(median: 0.0 (0.0, 2.0)); p = 0.001). DMFT ≥ 1 was significantly associated with SHS ≥ 10 years (adjusted odds ratio: 1.50, 95% confidence interval: 1.20-1.87, p < 0.001). Long-term exposure to SHS (≥10 years) was associated with dental caries in permanent dentition among Japanese young adults.

    DOI: 10.3390/ijerph17228623

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  • 若年者と高齢者との間での口腔機能の比較

    澤田 ななみ, 竹内 倫子, 江國 大輔, 森田 学

    日本老年歯科医学会総会・学術大会プログラム・抄録集   31回   130 - 130   2020年11月

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    記述言語:日本語   出版者・発行元:(一社)日本老年歯科医学会  

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  • 基本チェックリストの口腔機能3項目と口腔機能との関連

    竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

    日本老年歯科医学会総会・学術大会プログラム・抄録集   31回   102 - 102   2020年11月

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    記述言語:日本語   出版者・発行元:(一社)日本老年歯科医学会  

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  • 高校生におけるDMF歯数の増加と口腔保健行動との関連

    澤田 ななみ, 竹内 倫子, 田畑 綾乃, 江國 大輔, 森田 学

    口腔衛生学会雑誌   70 ( 4 )   190 - 195   2020年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

    これまで幼児や小・中学生のう蝕発症の要因に関する報告は多いが、高校生では少なく、縦断研究はほとんどない。本研究では高校生のう蝕発症に関連する要因を調べることを目的に、2年間の前向きコホート研究を行った。対象者は岡山市内の私立高等学校で、2016年4月(1年生)(ベースライン)と2018年4月(3年生)(再評価)に定期健康診断で歯・口腔の健康診断を受診した生徒とした。口腔内診査と質問票調査を行い、2年間のDMF歯数増加とベースライン時の口腔保健行動との関連をロジスティック回帰分析で検討した。160名(男子62名、女子98名)(平均年齢15.0±0.24歳)を分析した。ベースライン時のDMF歯数は0.86±1.75であった。DMF歯数増加群は72名(45.0%)であった。ロジスティック回帰分析の結果から、DMF歯数増加と有意な関連が認められたのは、「1年生時のDMF歯数が1以上」「フッ素入り歯磨剤を使用しない」「糖質ゼロ等表示食品を選ぶ」および「過去1年間に歯科医院で歯のそうじを受けた」の4項目であった(p<0.05)。結論として、高校生において、フッ素入り歯磨剤を使用することがう蝕発症を抑制する可能性が示唆された。(著者抄録)

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  • 福祉施設利用高齢者における日常生活自立度と口腔機能との関係

    中島 千穂, 竹内 倫子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   70 ( 4 )   215 - 221   2020年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

    日常生活動作ADLと現在歯数、咀嚼能力、オーラルディアドコキネシス(ODK)との関連が報告されているが、一定の見解を得られていない。また、過去の研究では単一施設内での報告が多く、複数施設での報告は少ない。本研究の目的は多施設で口腔機能とADLとの関連を調べることであった。岡山県北部の6ヶ所の福祉施設を利用した高齢者61名を対象とし、年齢、性別、要介護度、基礎疾患、ADL、食事形態、現在歯数、義歯の使用の有無、舌圧、ODKを調査した。食形態は「常食」と「常食以外」の2群に分類し、ODKは「/pa/」「/ta/」「/ka/」それぞれを「4.0回/秒未満」「4.0回/秒以上」の2群に分類した。ADLはランクJを「生活自立群」、ランクAを「準寝たきり群」、ランクBおよびCを「寝たきり群」と3群に分類した。ADLと年齢の関連はKruskal-Wallis検定を用い、ADLと年齢以外の各項目、ODKと食形態の関連はFisherの正確確率検定を用いて分析した。その結果、ADLの重症度は要介護度、糖尿病、骨関節疾患、食形態に加え、ODK「/pa/」「/ka/」との間に有意な関連がみられた。また、ODKすべての音節と食形態との間に有意な関連がみられた。ODKの低下が食形態の変化を介して栄養状態の悪化を招き、ADLの重症度に関連した可能性が示された。(著者抄録)

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  • 1歳6ヵ月児および2歳6ヵ月児におけるう蝕発生要因の比較

    竹内 倫子, 江國 大輔, 外山 直樹, 森田 学

    日本公衆衛生学会総会抄録集   79回   367 - 367   2020年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • Horseradish peroxidase interacts with the cell wall peptidoglycans on oral bacteria. 査読 国際誌

    Hirofumi Mizuno, Eiji Takayama, Ayano Satoh, Takeshi Into, Masanori Adachi, Daisuke Ekuni, Koji Yashiro, Masako Mizuno-Kamiya, Motohiko Nagayama, Seitaro Saku, Takaaki Tomofuji, Yutaka Doi, Yukitaka Murakami, Nobuo Kondoh, Manabu Morita

    Experimental and therapeutic medicine   20 ( 3 )   2822 - 2827   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Spandidos Publications  

    Salivary peroxidase and myeloperoxidase are known to display antibacterial activity against oral microbes, and previous indications have pointed to the possibility that horseradish peroxidase (HRP) adsorbs onto the membrane of the major oral streptococci, Streptococcus mutans and Streptococcus sanguinis (S. sanguinis). However, the mechanism of interaction between HRP and the bacterial cell wall component is unclear. Dental plaques containing salivary glycoproteins and extracellular microbial products are visualized with 'dental plaque disclosing agent', and are controlled within dental therapy. However, current 'dental plaque disclosing agents' are difficult to evaluate with just dental plaques, since they stain and disclose not only dental plaques but also pellicle formed with salivary glycoproteins on a tooth surface. In this present study, we have demonstrated that HRP interacted with the cell wall component of the major gram-positive bacterial peptidoglycan, but not the major cell wall component of gram-negative bacteria lipopolysaccharide. Furthermore, we observed that the adsorbed HRP labeled with fluorescence was detected on the major oral gram-positive strains S. sanguinis and Streptococcus salivarius (S. salivarius), but not on a gram-negative strain, Escherichia coli (E. coli). Furthermore, we have demonstrated that the combination of HRP and chromogenic substrate clearly disclosed the dental plaques and the biofilm developed by S. sanguinis, S. salivarius and the major gram-postive bacteria Lactobacillus casei on tooth surfaces, and slightly disclosed the biofilm by E. coli. The combination of HRP and chromogenic substrate did not stain either the dental pellicle with the salivary glycoprotein mucin, or naked tooth surfaces. These results have suggested the possibility that the adsorption activity of HRP not only contributes to the evaluation of dental plaque, but that enzymatic activity of HRP may also contribute to improve dental hygiene.

    DOI: 10.3892/etm.2020.9016

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  • Horseradish peroxidase interacts with the cell wall peptidoglycans on oral bacteria. 国際誌

    Hirofumi Mizuno, Eiji Takayama, Ayano Satoh, Takeshi Into, Masanori Adachi, Daisuke Ekuni, Koji Yashiro, Masako Mizuno-Kamiya, Motohiko Nagayama, Seitaro Saku, Takaaki Tomofuji, Yutaka Doi, Yukitaka Murakami, Nobuo Kondoh, Manabu Morita

    Experimental and therapeutic medicine   20 ( 3 )   2822 - 2827   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Salivary peroxidase and myeloperoxidase are known to display antibacterial activity against oral microbes, and previous indications have pointed to the possibility that horseradish peroxidase (HRP) adsorbs onto the membrane of the major oral streptococci, Streptococcus mutans and Streptococcus sanguinis (S. sanguinis). However, the mechanism of interaction between HRP and the bacterial cell wall component is unclear. Dental plaques containing salivary glycoproteins and extracellular microbial products are visualized with 'dental plaque disclosing agent', and are controlled within dental therapy. However, current 'dental plaque disclosing agents' are difficult to evaluate with just dental plaques, since they stain and disclose not only dental plaques but also pellicle formed with salivary glycoproteins on a tooth surface. In this present study, we have demonstrated that HRP interacted with the cell wall component of the major gram-positive bacterial peptidoglycan, but not the major cell wall component of gram-negative bacteria lipopolysaccharide. Furthermore, we observed that the adsorbed HRP labeled with fluorescence was detected on the major oral gram-positive strains S. sanguinis and Streptococcus salivarius (S. salivarius), but not on a gram-negative strain, Escherichia coli (E. coli). Furthermore, we have demonstrated that the combination of HRP and chromogenic substrate clearly disclosed the dental plaques and the biofilm developed by S. sanguinis, S. salivarius and the major gram-postive bacteria Lactobacillus casei on tooth surfaces, and slightly disclosed the biofilm by E. coli. The combination of HRP and chromogenic substrate did not stain either the dental pellicle with the salivary glycoprotein mucin, or naked tooth surfaces. These results have suggested the possibility that the adsorption activity of HRP not only contributes to the evaluation of dental plaque, but that enzymatic activity of HRP may also contribute to improve dental hygiene.

    DOI: 10.3892/etm.2020.9016

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  • Horseradish peroxidase interacts with the cell wall peptidoglycans on oral bacteria. 国際誌

    Hirofumi Mizuno, Eiji Takayama, Ayano Satoh, Takeshi Into, Masanori Adachi, Daisuke Ekuni, Koji Yashiro, Masako Mizuno-Kamiya, Motohiko Nagayama, Seitaro Saku, Takaaki Tomofuji, Yutaka Doi, Yukitaka Murakami, Nobuo Kondoh, Manabu Morita

    Experimental and therapeutic medicine   20 ( 3 )   2822 - 2827   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Salivary peroxidase and myeloperoxidase are known to display antibacterial activity against oral microbes, and previous indications have pointed to the possibility that horseradish peroxidase (HRP) adsorbs onto the membrane of the major oral streptococci, Streptococcus mutans and Streptococcus sanguinis (S. sanguinis). However, the mechanism of interaction between HRP and the bacterial cell wall component is unclear. Dental plaques containing salivary glycoproteins and extracellular microbial products are visualized with 'dental plaque disclosing agent', and are controlled within dental therapy. However, current 'dental plaque disclosing agents' are difficult to evaluate with just dental plaques, since they stain and disclose not only dental plaques but also pellicle formed with salivary glycoproteins on a tooth surface. In this present study, we have demonstrated that HRP interacted with the cell wall component of the major gram-positive bacterial peptidoglycan, but not the major cell wall component of gram-negative bacteria lipopolysaccharide. Furthermore, we observed that the adsorbed HRP labeled with fluorescence was detected on the major oral gram-positive strains S. sanguinis and Streptococcus salivarius (S. salivarius), but not on a gram-negative strain, Escherichia coli (E. coli). Furthermore, we have demonstrated that the combination of HRP and chromogenic substrate clearly disclosed the dental plaques and the biofilm developed by S. sanguinis, S. salivarius and the major gram-postive bacteria Lactobacillus casei on tooth surfaces, and slightly disclosed the biofilm by E. coli. The combination of HRP and chromogenic substrate did not stain either the dental pellicle with the salivary glycoprotein mucin, or naked tooth surfaces. These results have suggested the possibility that the adsorption activity of HRP not only contributes to the evaluation of dental plaque, but that enzymatic activity of HRP may also contribute to improve dental hygiene.

    DOI: 10.3892/etm.2020.9016

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  • 抗菌的光線力学療法が歯肉縁下細菌叢の形成に与える影響について

    入江 浩一郎, 田畑 綾乃, 内田 瑶子, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   70 ( 3 )   144 - 151   2020年7月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

    非侵襲的な歯周病の治療法として抗菌的光線力学療法(antimicrobial photodynamic therapy;a-PDT)が注目されている。a-PDTの臨床効果を評価した報告は多数あるが、細菌叢に着目した研究は少ない。本研究では、歯肉炎を有する学生ボランティアを対象に、a-PDTによる歯肉縁下細菌叢の形成に与える影響について検討した。下顎第一、第二大臼歯部に歯肉炎を有する男子学生6名(平均年齢24.2±0.98歳)を対象にした。被験者の下顎第一、第二大臼歯部を左右で分割し、照射(a-PDT)群もしくは対照群をコイントスで割付けた。照射群ではスケーリングとa-PDTを、そして対照群ではスケーリングを行った。処置開始前と処置の2週間後に、下顎第一、第二大臼歯部歯間部歯肉縁下から細菌を採取した。サンプルからDNAを抽出し、次世代シーケンス解析を行った。その結果、Red complexに属する細菌の割合は、照射群において処置前と処置後との間で有意な減少を認めた(p<0.05)。一方、対照群においては、処置前後で有意差は認められなかった。本研究から、スケーリングとa-PDTを併用した場合、歯肉縁下細菌叢中の歯周病原性の高い細菌の占める割合が有意に減少することが示された。(著者抄録)

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  • Caries Increment and Salivary Microbiome during University Life: A Prospective Cohort Study. 国際誌

    Yoko Uchida-Fukuhara, Daisuke Ekuni, Md Monirul Islam, Kota Kataoka, Ayano Taniguchi-Tabata, Daiki Fukuhara, Naoki Toyama, Terumasa Kobayashi, Kohei Fujimori, Nanami Sawada, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   17 ( 10 )   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purpose of this 3-year prospective cohort study was to explore the relationship between an increase in dental caries and oral microbiome among Japanese university students. We analyzed 487 students who volunteered to receive oral examinations and answer baseline (2013) and follow-up (2016) questionnaires. Of these students, salivary samples were randomly collected from 55 students at follow-up and analyzed using next-generation sequencing. Students were divided into two groups: increased group (Δdecayed, missing, and filled teeth (ΔDMFT) score increased during the 3-year period) and non-increased group (ΔDMFT did not increase). Thirteen phyla, 21 classes, 32 orders, 48 families, 72 genera, and 156 species were identified. Microbial diversity in the increased group (n = 14) was similar to that in the non-increased group (n = 41). Relative abundances of the family Prevotellaceae (p = 0.007) and genera Alloprevotella (p = 0.007) and Dialister (p = 0.039) were enriched in the increased group compared with the non-increased group. Some bacterial taxonomic clades were differentially present between the two groups. These results may contribute to the development of new dental caries prevention strategies, including the development of detection kits and enlightenment activities for these bacteria.

    DOI: 10.3390/ijerph17103713

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  • 歯周病試験紙を応用した職域における歯科検診の試み

    山本 龍生, 渕田 慎也, 持田 悠貴, 江國 大輔, 森田 学, 恒石 美登里

    産業衛生学雑誌   62 ( 臨増 )   406 - 406   2020年5月

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    記述言語:日本語   出版者・発行元:(公社)日本産業衛生学会  

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  • Association between Sleep Quality and Duration and Periodontal Disease among University Students: A Cross-Sectional Study. 国際誌

    Md Monirul Islam, Daisuke Ekuni, Naoki Toyama, Ayano Taniguchi-Tabata, Kota Kataoka, Yoko Uchida-Fukuhara, Daiki Fukuhara, Hikari Saho, Nanami Sawada, Yukiho Nakashima, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   17 ( 9 )   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purpose of this cross-sectional study was to investigate the association between sleep quality and duration, and periodontal disease among a group of young Japanese university students. First-year students (n = 1934) at Okayama University who voluntarily underwent oral health examinations were included in the analysis. Sleep quality and duration were assessed by the Japanese version of the Pittsburgh Sleep Quality Index. Dentists examined Oral Hygiene Index-Simplified (OHI-S), probing pocket depth (PPD), and percentage of sites with bleeding on probing (BOP). Periodontal disease was defined as presence of PPD ≥ 4 mm and BOP ≥ 30%. Overall, 283 (14.6%) students had periodontal disease. Poor sleep quality was observed among 372 (19.2%) students. Mean (± standard deviation) sleep duration was 7.1 ± 1.1 (hours/night). In the logistic regression analysis, periodontal disease was significantly associated with OHI-S (odds ratio [OR]: 2.30, 95% confident interval [CI]: 1.83-2.90; p < 0.001), but not sleep quality (OR: 1.09, 95% CI: 0.79-1.53; p = 0.577) or sleep duration (OR: 0.98, CI: 0.87-1.10; p = 0.717). In conclusion, sleep quality and duration were not associated with periodontal disease among this group of young Japanese university students.

    DOI: 10.3390/ijerph17093034

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  • Associations between sleep bruxism, sleep quality, and exposure to secondhand smoke in Japanese young adults: a cross-sectional study. 国際誌

    Naoki Toyama, Daisuke Ekuni, Ayano Taniguchi-Tabata, Toshiki Yoneda, Kota Kataoka, Aya Yokoi, Yoko Uchida, Daiki Fukuhara, Hikari Saho, Islam Md Monirul, Nanami Sawada, Yukiho Nakashima, Yoshiaki Iwasaki, Manabu Morita

    Sleep medicine   68   57 - 62   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: Sleep bruxism, a major sleep disorder that causes serious harm to oral health, is considered a multifactorial disease. Sleep bruxism can be induced by smoking, which also adversely affects sleep quality. The objective of present study was to clarify the associations between sleep bruxism, sleep quality, and exposure to secondhand smoke (SHS). METHODS: To assess the prevalence of sleep bruxism, sleep quality, and SHS exposure, we conducted oral examinations and self-report questionnaires on university students in Japan. Sleep bruxism and quality were screened using the Japanese version of the Pittsburgh Sleep Quality Index (PSQI) and the third edition of the International Classification of Sleep Disorders (ICSD-3). The inclusion criteria were adults aged between 18 and 19 years, non-smokers and non-alcohol drinkers. The exclusion criteria was failing to complete the questionnaire in full. RESULTS: We analyzed a total of 1781 Japanese young adults. Young adult females who had been exposed to SHS had worse sleep quality (p = 0.019) than those who had not. Young adult female with worse sleep quality showed a higher prevalence of sleep bruxism (p = 0.034) than those with better sleep quality. Using structural equation modeling, direct associations were identified between SHS exposure and poor sleep quality (standardized coefficients, 0.153; p = 0.008) and between sleep bruxism and poor sleep quality (standardized coefficients, 0.187; p = 0.022) in young adult females. However, no association was found among young adult males. CONCLUSION: SHS exposure is indirectly associated with sleep bruxism through poor sleep quality in Japanese young adult females.

    DOI: 10.1016/j.sleep.2019.09.003

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  • Relationship of Salivary Microbiome with the Worsening of the Periodontal Health Status in Young Adults: A 3-Year Cohort Study. 国際誌

    Md Monirul Islam, Daisuke Ekuni, Naoki Toyama, Terumasa Kobayashi, Kohei Fujimori, Yoko Uchida, Daiki Fukuhara, Ayano Taniguchi-Tabata, Kota Kataoka, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   17 ( 5 )   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purpose of this prospective cohort study was to investigate the influence of the salivary microbiome on the worsening of the periodontal health status among Japanese young adults. We assessed the data of systemically healthy and non-smoking young (18-22 years) university students (n = 457) from Okayama University at baseline (2013) and follow-up (2016). The worsening group was defined based on an increase in the percentage of bleeding on probing (%BOP) or an increase in probing pocket depth (PPD) from <4 mm to ≥4 mm. Unstimulated saliva samples were randomly collected from 69 students for microbiome analysis at follow-up. The salivary microbiome was assessed through 16S rRNA metagenomic sequencing. The type of community in the salivary microbiome clustered by statistical analysis and diversity was not significantly associated with the worsening of the periodontal health status in cases of increasing %BOP and PPD (p > 0.05). The prevalence of some species was significantly higher in the worsening group than in the non-worsening group (p < 0.05) in both cases. The worsening of the periodontal health status was associated with some species, but not the type of community and diversity in the salivary microbiome among Japanese young adults.

    DOI: 10.3390/ijerph17051764

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  • 大学生における睡眠の質とストレスが歯周病に及ぼす影響(Influence of sleep quality and stress on periodontal disease among university students)

    Islam Monirul, Ekuni Daisuke, Toyama Naoki, Tabata Ayano, Kataoka Kota, Uchida Yoko, Fukuhara Daiki, Saho Hikari, Sawada Nanami, Nakashima Yukiho, Morita Manabu

    口腔衛生学会雑誌   70 ( 増刊 )   159 - 159   2020年3月

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    記述言語:英語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 慢性歯周炎患者の歯周状態の悪化に関連する唾液中miRNAの探索 コホート研究

    藤森 浩平, 米田 俊樹, 杉浦 嘉雄, 丸山 貴之, 江國 大輔, 東 哲司, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   70 ( 増刊 )   158 - 158   2020年3月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 大学入学後の居住形態の変化と歯周病発症との関連(前向きコホート研究) 査読

    中原 桃子, 田畑 綾乃, 外山 直樹, 米田 俊樹, 片岡 広太, 佐保 輝, Islam Monirul, 横井 彩, 江國 大輔, 森田 学

    口腔衛生学会雑誌   70 ( 増刊 )   159 - 159   2020年3月

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  • Influence of sleep quality and stress on periodontal disease among university students(和訳中) 査読

    Islam Monirul, Ekuni Daisuke, Toyama Naoki, Tabata Ayano, Kataoka Kota, Uchida Yoko, Fukuhara Daiki, Saho Hikari, Sawada Nanami, Nakashima Yukiho, Morita Manabu

    口腔衛生学会雑誌   70 ( 増刊 )   159 - 159   2020年3月

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  • 大学入学後の居住形態の変化と歯周病発症との関連(前向きコホート研究)

    中原 桃子, 田畑 綾乃, 外山 直樹, 米田 俊樹, 片岡 広太, 佐保 輝, Islam Monirul, 横井 彩, 江國 大輔, 森田 学

    口腔衛生学会雑誌   70 ( 増刊 )   159 - 159   2020年3月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • Association between intensive health guidance focusing on eating quickly and metabolic syndrome in Japanese middle-aged citizens. 国際誌

    Daisuke Ekuni, Michiko Furuta, Toshihide Kimura, Naoki Toyama, Daiki Fukuhara, Yoko Uchida, Nanami Sawada, Manabu Morita

    Eating and weight disorders : EWD   25 ( 1 )   91 - 98   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: The purpose of this intervention study was to investigate whether intensive health guidance focusing on eating quickly can prevent metabolic syndrome (MetS) more effectively than standard routine guidance in Japanese citizens living in rural areas. METHODS: This controlled, non-randomized, intervention study analyzed 141 participants with MetS at baseline. Participants in the intervention group received health guidance focusing on eating quickly and standard health guidance about MetS in accordance with the guidelines of the Ministry of Health, Labour and Welfare in Japan, whereas participants in the control group received only standard health guidance about MetS. The primary study outcome was the prevalence of MetS at a 1-year follow-up. RESULTS: At 1-year follow-up, the prevalence of MetS in the intervention group was significantly lower than that in the control group (p = 0.003). The decreases in body weight, body mass index, waist circumference and triglycerides from baseline to 1 year were significantly greater in the intervention group than in the control group (p < 0.05). CONCLUSION: Intensive health guidance focusing on eating quickly is more effective for improving MetS than standard Japanese health guidance alone. LEVEL OF EVIDENCE: Level II, Evidence obtained from well-designed controlled trials without randomization. TRIAL REGISTRY NAME, REGISTRATION IDENTIFICATION NUMBER, AND URL FOR THE REGISTRY: UMIN, UMIN000030600, http://www.umin.ac.jp/ctr/index-j.htm.

    DOI: 10.1007/s40519-018-0522-1

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  • Association between maternal periodontal status and ultrasonographic measurement of fetal growth: A longitudinal study. 国際誌

    Ayano Taniguchi-Tabata, Noriko Takeuchi, Yoko Uchida, Daisuke Ekuni, Manabu Morita

    Scientific reports   10 ( 1 )   1402 - 1402   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The aim of this prospective cohort study was to investigate the association between intrauterine fetal growth patterns and periodontal status in pregnant women. Fifty-three pregnant women were recruited. Periodontitis was diagnosed based on probing pocket depth and clinical attachment level. Maternal urinary 8-hydroxy-2'-deoxyguanosine levels and body mass index were recorded. Ultrasonographic measurements of the biparietal diameter (BPD), abdominal circumference (AC), and femur length (FL) were recorded, and estimated fetal weight (EFW) was calculated. In addition, approximation spline curves of BPD, AC, FL, and EFW were obtained throughout the gestation period. Recorded delivery outcomes were gestational age (GA), birth weight and length, sex, placental weight, and umbilical cord length. Forty-four participants (34.1 ± 4.9 years) were analyzed. Mean neonatal birth weight was 2906.0 ± 544.4 g. On multiple regression analysis, birth weight was related with periodontitis (standardized β = -0.21, P = 0.001). For EFW and BPD, the curves of the periodontitis group were located lower than those of the non-periodontitis group, with significant differences after 32 weeks and 20 weeks of GA, respectively. In conclusion, periodontal treatment before conception may be recommended and a good periodontal condition in the early stage of pregnancy at the latest is desirable for infant growth.

    DOI: 10.1038/s41598-020-58396-7

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  • Effects of Coffee Intake on Oxidative Stress During Aging-related Alterations in Periodontal Tissue

    TERUMASA KOBAYASHI, TAKAYUKI MARUYAMA, TOSHIKI YONEDA, HISATAKA MIYAI, TETSUJI AZUMA, TAKAAKI TOMOFUJI, DAISUKE EKUNI, MANABU MORITA

    In Vivo   34 ( 2 )   615 - 622   2020年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Anticancer Research USA Inc.  

    Background/Aim: The purpose of this study was to determine the anti-aging effects of coffee intake on oxidative stress in rat periodontal tissue and alveolar bone loss. Materials and Methods: Male Fischer 344 rats (8 weeks old) were randomized to four groups; the baseline group immediately sacrificed, the control group fed with normal powdered food for 8 weeks, and the experimental groups fed with powdered food containing 0.62% or 1.36% coffee components for 8 weeks. Results: Alveolar bone loss and gingival level of 8-hydroxydeoxyguanosine were significantly lower in the 1.36% coffee group than in the control group. Nuclear factor erythroid 2-related factor 2 translocation to the nucleus was significantly higher in the 1.36% coffee group than in the control group. Conclusion: Continuous intake of 1.36% coffee could prevent age-related oxidative stress in the periodontal tissue and alveolar bone loss, possibly by up-regulating the Nrf2 signaling pathway.

    DOI: 10.21873/invivo.11815

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  • Detection of Serum miRNAs Affecting Liver Apoptosis in a Periodontitis Rat Model

    YOSHIO SUGIURA, TOSHIKI YONEDA, KOHEI FUJIMORI, TAKAYUKI MARUYAMA, HISATAKA MIYAI, TERUMASA KOBAYASHI, DAISUKE EKUNI, TAKAAKI TOMOFUJI, MANABU MORITA

    In Vivo   34 ( 1 )   117 - 123   2020年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Anticancer Research USA Inc.  

    Background/Aim: miRNA molecules have been attracting attention as genetic modifiers between organs. We examined the relationship between serum miRNA and targeted liver mRNA profiles in a periodontitis rat model, and the influence of periodontitis on the liver. Materials and Methods: Male Wistar rats (n=16, 8 weeks old) were randomly divided into two groups (8 rats each): control and periodontitis (ligature placement for 4 weeks). Serum miRNA and liver mRNA profiles were compared. Results: Periodontal destruction and hepatocyte apoptosis were induced in the periodontitis group. Microarray analysis indicated that 52 serum miRNAs and 33 liver mRNAs were expressed with a >1.5-fold change (FC) and a >2.0-FC (p<0.05), respectively, between the two groups. From the miRNA target genes, 12 genes equivalented to liver mRNAs with a >2.0-FC, among which, Hyou1, Chac1, and Bloc1s3 have apoptotic functions in our model. miRNAs upstream of these 3 mRNAs are miR-3591, miR-181a-2-3p and miR-6321. Conclusion: miR-3591, miR-181a-2-3p and miR-6321 induced hepatocyte apoptosis in our periodontitis rat model.

    DOI: 10.21873/invivo.11752

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  • Relationship between oral hygiene knowledge, source of oral hygiene knowledge and oral hygiene behavior in Japanese university students: A prospective cohort study. 査読 国際誌

    Daiki Fukuhara, Daisuke Ekuni, Kota Kataoka, Ayano Taniguchi-Tabata, Yoko Uchida-Fukuhara, Naoki Toyama, Toshiki Yoneda, Yoshio Sugiura, Md Monirul Islam, Hikari Saho, Yoshiaki Iwasaki, Manabu Morita

    PloS one   15 ( 7 )   e0236259   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The aim of this prospective cohort study was to examine whether oral hygiene knowledge, and the source of that knowledge, affect oral hygiene behavior in university students in Japan. An oral exam and questionnaire survey developed to evaluate oral hygiene knowledge, the source of that knowledge, and oral hygiene behavior, such as the frequency of tooth brushing and regular dental checkups and the use of dental floss, was conducted on university student volunteers. In total, 310 students with poor tooth brushing behavior (frequency of tooth brushing per day [≤ once]), 1,963 who did not use dental floss, and 1,882 who did not receive regular dental checkup during the past year were selected. Among these students, 50, 364, and 343 in each respective category were analyzed in over the 3-year study period (follow-up rates: 16.1%, 18.5%, and 18.2%, respectively). The odds ratios (ORs) and 95% confidence intervals (CIs) for oral hygiene behavior were calculated based on oral hygiene knowledge and the source of that knowledge using logistic regression models. The results showed that dental clinics were the most common (> 50%) source of oral hygiene knowledge, and that a more frequent use of dental floss was significantly associated with dental clinics being a source of oral hygiene knowledge (OR, 4.11; 95%CI, 1.871-9.029; p < 0.001). In addition, a significant association was seen between dental clinics being a source of oral hygiene knowledge and more frequent regular dental checkups (OR, 13.626; 95%CI, 5.971-31.095; p < 0.001). These findings suggest the existence of a relationship between dental clinics being the most common source of oral hygiene knowledge and improved oral hygiene behavior in Japanese university students.

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  • Effects of Coffee Intake on Oxidative Stress During Aging-related Alterations in Periodontal Tissue. 国際誌

    Terumasa Kobayashi, Takayuki Maruyama, Toshiki Yoneda, Hisataka Miyai, Tetsuji Azuma, Takaaki Tomofuji, Daisuke Ekuni, Manabu Morita

    In vivo (Athens, Greece)   34 ( 2 )   615 - 622   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND/AIM: The purpose of this study was to determine the anti-aging effects of coffee intake on oxidative stress in rat periodontal tissue and alveolar bone loss. MATERIALS AND METHODS: Male Fischer 344 rats (8 weeks old) were randomized to four groups; the baseline group immediately sacrificed, the control group fed with normal powdered food for 8 weeks, and the experimental groups fed with powdered food containing 0.62% or 1.36% coffee components for 8 weeks. RESULTS: Alveolar bone loss and gingival level of 8-hydroxydeoxyguanosine were significantly lower in the 1.36% coffee group than in the control group. Nuclear factor erythroid 2-related factor 2 translocation to the nucleus was significantly higher in the 1.36% coffee group than in the control group. CONCLUSION: Continuous intake of 1.36% coffee could prevent age-related oxidative stress in the periodontal tissue and alveolar bone loss, possibly by up-regulating the Nrf2 signaling pathway.

    DOI: 10.21873/invivo.11815

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  • Detection of Serum miRNAs Affecting Liver Apoptosis in a Periodontitis Rat Model. 国際誌

    Yoshio Sugiura, Toshiki Yoneda, Kohei Fujimori, Takayuki Maruyama, Hisataka Miyai, Terumasa Kobayashi, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    In vivo (Athens, Greece)   34 ( 1 )   117 - 123   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND/AIM: miRNA molecules have been attracting attention as genetic modifiers between organs. We examined the relationship between serum miRNA and targeted liver mRNA profiles in a periodontitis rat model, and the influence of periodontitis on the liver. MATERIALS AND METHODS: Male Wistar rats (n=16, 8 weeks old) were randomly divided into two groups (8 rats each): control and periodontitis (ligature placement for 4 weeks). Serum miRNA and liver mRNA profiles were compared. RESULTS: Periodontal destruction and hepatocyte apoptosis were induced in the periodontitis group. Microarray analysis indicated that 52 serum miRNAs and 33 liver mRNAs were expressed with a >1.5-fold change (FC) and a >2.0-FC (p<0.05), respectively, between the two groups. From the miRNA target genes, 12 genes equivalented to liver mRNAs with a >2.0-FC, among which, Hyou1, Chac1, and Bloc1s3 have apoptotic functions in our model. miRNAs upstream of these 3 mRNAs are miR-3591, miR-181a-2-3p and miR-6321. CONCLUSION: miR-3591, miR-181a-2-3p and miR-6321 induced hepatocyte apoptosis in our periodontitis rat model.

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  • 大学生における歯科に関する知識、歯科に関する知識の情報源及び口腔保健行動との関連:前向きコホート研究

    福原 大樹, 江國 大輔, 片岡 広太, 田畑 綾乃, 内田 瑶子, 外山 直樹, 米田 俊樹, 杉浦 嘉雄, イスラム・モハマド・モニルル, 佐保 輝, 森田 学

    岡山歯学会雑誌   38 ( 2 )   93 - 93   2019年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 大学生における歯科に関する知識、歯科に関する知識の情報源及び口腔保健行動との関連:前向きコホート研究 査読

    福原 大樹, 江國 大輔, 片岡 広太, 田畑 綾乃, 内田 瑶子, 外山 直樹, 米田 俊樹, 杉浦 嘉雄, イスラム・モハマド・モニルル, 佐保 輝, 森田 学

    岡山歯学会雑誌   38 ( 2 )   93 - 93   2019年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • Structural equation modeling to detect predictors of oral health-related quality of life among Japanese university students: a prospective cohort study. 国際誌

    Hikari Saho, Daisuke Ekuni, Kota Kataoka, Ayano Taniguchi-Tabata, Naoki Toyama, Yoshio Sugiura, Md Monirul Islam, Yoshiaki Iwasaki, Manabu Morita

    Quality of life research : an international journal of quality of life aspects of treatment, care and rehabilitation   28 ( 12 )   3213 - 3224   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PURPOSE: This prospective cohort study of Japanese university students aimed to identify factors that might affect oral health-related quality of life (OHRQoL) and longitudinal relationships over a period of 3 years. METHODS: Students (n = 487) provided complete data before entering and 3 years later (before university graduation) participated in the present study. Decayed, missing, and filled teeth (DMFT) scores, community periodontal index, ratios (%) of teeth with bleeding on probing, and malocclusion were determined during oral examinations. The questionnaire addressed age, sex, self-rated oral health, oral health behavior, subjective oral symptoms, and OHRQoL determined using the oral health impact profile (OHIP)-14. Associations were analyzed using structural equation modeling (SEM). RESULTS: The OHRQoL significantly worsened according to OHIP-14 total score (p = 0.001). The final model in the symptoms of SEM analysis showed that OHRQoL at follow-up positively correlated with OHRQoL at baseline. Self-rated oral health was directly associated with the OHRQoL at baseline (p < 0.05). CONCLUSIONS: This study determined that OHRQoL at baseline was a direct predictor, and that self-rated oral health were indirect predictors of OHRQoL at follow-up among Japanese university students.

    DOI: 10.1007/s11136-019-02251-4

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  • Quasi-Randomized Trial of Effects of Perioperative Oral Hygiene Instruction on Inpatients with Heart Diseases Using a Behavioral Six-Step Method. 国際誌

    Chie Omori, Daisuke Ekuni, Yumiko Ohbayashi, Minoru Miyake, Manabu Morita

    International journal of environmental research and public health   16 ( 21 )   2019年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The assessor-blinded, parallel-design, quasi-randomized study (alternating allocation) aimed to determine the effects of the six-step method on postoperative numbers of oral bacteria, periodontal status, and atrial fibrillation (AF) among inpatients with heart diseases and periodontitis. Seventy inpatients who received preoperative periodontal treatment were quasi-randomly assigned to intervention and control groups at University Hospital. The intervention group received intensive oral hygiene instruction using a six-step method for 15 minutes per week and the control group received routine oral hygiene instruction. Significantly fewer oral bacteria were identified on the tongue at discharge compared with baseline in the intervention than the control group (ANCOVA) (large effect size, p = 0.02). Changes in scores for self-efficacy, plaque scores, probed pocket depth, and bleeding on probing between baseline and discharge were significantly greater in the intervention, than in the control group (p < 0.05). The period of postoperative AF (days) was significantly shorter in the intervention, than in the control group (p = 0.019). In conclusion, oral hygiene instruction using the six-step method decreased the numbers of oral bacteria on the tongue and improved self-efficacy, oral health behaviors, oral hygiene status, periodontal status, and period of postoperative AF among inpatients with periodontitis and heart diseases.

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  • 舌口唇運動機能は身体フレイルに関連する

    竹内 倫子, 江國 大輔, 田畑 綾乃, 外山 直樹, 森田 学

    日本公衆衛生学会総会抄録集   78回   231 - 231   2019年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • Influence of Occupational Stress and Coping Style on Periodontitis among Japanese Workers: A Cross-Sectional Study. 国際誌

    Md Monirul Islam, Daisuke Ekuni, Toshiki Yoneda, Aya Yokoi, Manabu Morita

    International journal of environmental research and public health   16 ( 19 )   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The aim of this cross-sectional study was to evaluate the association between the influence of occupational stress and coping style on periodontitis among Japanese workers. The study sample included 738 workers (age range: 19-65 years) at a manufacturing company in Kagawa Prefecture, Japan. To analyze occupational stress and coping style, all participants answered a self-report questionnaire composed of items on their work environment and oral health behavior. Oral examinations were performed by calibrated dentists. Among all workers, 492 (66.7%) workers were diagnosed with periodontitis, and 50 (6.8%) were diagnosed with a high stress-low coping condition. Significant differences (p < 0.05) were observed between the periodontitis and non-periodontitis groups in terms of age, gender, body mass index, smoking status, daily alcohol drinking, monthly overtime work, worker type, and stress-coping style. Logistic regression analysis showed that a high stress-low coping condition was associated with an increased risk of periodontitis (odds ratio: 2.79, 95% confidence interval: 1.05-7.43, p = 0.039). These findings suggest that a high stress-low coping condition is associated with periodontitis among the 19-65 years of age group of Japanese workers.

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  • Incidence of the Acute Symptom of Chronic Periodontal Disease in Patients Undergoing Supportive Periodontal Therapy: A 5-Year Study Evaluating Climate Variables. 国際誌

    Hikari Saho, Noriko Takeuchi, Daisuke Ekuni, Manabu Morita

    International journal of environmental research and public health   16 ( 17 )   2019年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Although patients under supportive periodontal therapy (SPT) have a stable periodontal condition, the acute symptom of chronic periodontal disease occasionally occurs without a clear reason. Therefore, in the present study, to obtain a better understanding of this relationship in patients undergoing SPT, we hypothesized that the acute symptom of chronic periodontal disease might be affected by climate factors. We conducted a questionnaire study and carried out oral examinations on patients undergoing SPT who had been diagnosed as having the acute symptom of chronic periodontal disease. We collected climate data from the local climate office in Okayama city, Japan. We predicted parameters that affect the acute symptom of chronic periodontal disease with unidentified cause and divided patients into high and low groups in terms of climate predictors. Then we defined the cut-off values of parameters showing significant differences in the incidence of the acute symptom of chronic periodontal disease. The incidence of the acute symptom of chronic periodontal disease with unidentified cause was significantly different when the cases were classified according to the maximum hourly decrease in barometric pressure (1.5 and 1.9 hPa) (p = 0.04 and p = 0.03, respectively). This suggests that climate variables could be predictors of the acute symptom of chronic periodontal disease. Therefore, gaining a better understanding of these factors could help periodontal patients undergoing SPT prepare to avoid the acute symptom of chronic periodontal disease.

    DOI: 10.3390/ijerph16173070

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  • Change in tongue pressure and the related factors after esophagectomy: a short-term, longitudinal study.

    Aya Yokoi, Daisuke Ekuni, Reiko Yamanaka, Hironobu Hata, Yasuhiro Shirakawa, Manabu Morita

    Esophagus : official journal of the Japan Esophageal Society   16 ( 3 )   300 - 308   2019年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Dysphagia is a prominent symptom after esophagectomy and may cause aspiration pneumonia. Swallowing evaluation after esophagectomy can predict and help control the incidence of postoperative pneumonia. The aim of this study was to clarify whether the change in tongue pressure was associated with any related factor and postoperative dysphagia/pneumonia in patients with esophageal cancer after esophagectomy. METHODS: Fifty-nine inpatients (41 males and 18 females; 33-77 years old) who underwent esophagectomy participated in this study. Measurement of tongue pressure and the repetitive saliva swallowing test (RSST) was performed before esophagectomy (baseline) and at 2 weeks postoperatively. The general data were collected from patients' medical records, including sex, age, type of cancer, cancer stage, location of cancer, operative approach, history of previous chemotherapy, surgical duration, amount of bleeding during surgery, incidences of postoperative complications, intubation period, period between surgery and initiation of oral alimentation, and intensive care unit (ICU) stay, blood chemical analysis, and lifestyle. RESULTS: Tongue pressure decreased significantly after esophagectomy (p = 0.011). The decrease of tongue pressure was significantly associated with length of ICU stay and preoperative tongue pressure on multiple regression analysis (p < 0.05). The decrease of tongue pressure in the RSST < 3 or postoperative pneumonia (+) group was significantly greater than in the RSST ≥ 3 (p = 0.003) or pneumonia (-) group (p = 0.021). CONCLUSIONS: The decrease in tongue pressure was significantly associated with the length of ICU stay, preoperative tongue pressure, and the incidence of dysphagia and pneumonia among inpatient after esophagectomy.

    DOI: 10.1007/s10388-019-00668-x

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  • Relationship between acetaldehyde concentration in mouth air and characteristics of microbiota of tongue dorsum in Japanese healthy adults: a cross-sectional study. 国際誌

    Aya Yokoi, Daisuke Ekuni, Hironobu Hata, Mayu Yamane-Takeuchi, Takayuki Maruyama, Reiko Yamanaka, Manabu Morita

    Journal of applied oral science : revista FOB   27   e20180635   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: Acetaldehyde, associated with consumption of alcoholic beverages, is known to be a carcinogen and to be related to the tongue dorsum. The aim of this study was to investigate the relationship between acetaldehyde concentration in mouth air and bacterial characteristics on the tongue dorsum. METHODOLOGY: Thirty-nine healthy volunteers participated in the study. Acetaldehyde concentrations in mouth air were evaluated by a high-sensitivity semiconductor gas sensor. A 16S rRNA gene sequencing technique was used to compare microbiomes between two groups, focusing on the six samples with the highest acetaldehyde concentrations (HG) and the six samples with lowest acetaldehyde concentrations (LG). RESULTS: Acetaldehyde concentration increased in correlation with the increase in bacterial count (p=0.048). The number of species observed in the oral microbiome of the HG was higher than that in the oral microbiome of the LG (p=0.011). The relative abundances of Gemella sanguinis, Veillonella parvula and Neisseria flavescens in the oral microbiome of the HG were higher than those in the oral microbiome of the LG (p<0.05). CONCLUSION: Acetaldehyde concentration in mouth air was associated with bacterial count, diversity of microbiome, and relative abundance of G. sanguinis, V. parvula, and N. flavescens.

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  • Relationship between acetaldehyde concentration in mouth air and characteristics of microbiota of tongue dorsum in Japanese healthy adults: a cross-sectional study. 査読 国際誌

    Yokoi A, Ekuni D, Hata H, Yamane-Takeuchi M, Maruyama T, Yamanaka R, Morita M

    Journal of applied oral science : revista FOB   27   e20180635   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1590/1678-7757-2018-0635

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  • Serum microRNAs and chronic periodontitis: A case-control study. 査読 国際誌

    Yoneda T, Tomofuji T, Ekuni D, Azuma T, Maruyama T, Fujimori K, Sugiura Y, Morita M

    Archives of oral biology   101   57 - 63   2019年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.archoralbio.2019.03.009

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  • 光線力学療法を併用した歯周治療によって歯周状態が改善した一症例

    江國 大輔, 中島 千穂, 森田 学

    口腔衛生学会雑誌   69 ( 2 )   98 - 105   2019年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

    本症例(女性、ベースライン時77歳7ヵ月)は、以前から重度歯周炎のため、Supportive Periodontal Therapy(SPT)を受けており、歯周状態は安定していた。しかし、#34部の歯周状態が悪化し、違和感を訴えるようになった。また、#45部の破折による排膿が認められた。そこで、従来のスケーリング・ルートプレーニング(SRP)に光線力学療法(Fotosan)を組み合わせることにした。ベースライン時に違和感を訴えている#34の動揺度は0で、頬側近心の歯周ポケット深さ(Probing Pocket Depth、PPD)は5mmであった。プロービング時の出血(Bleeding on probing、BOP)および排膿はなかった。#45の破折部の動揺度は0、PPDは9mmで、BOPおよび排膿が認められた。SRPと光線力学療法を併用した結果、3ヵ月後には主訴である#34部の違和感は消失した。頬側近心のPPDは2mmに軽減し、その後2年3ヵ月まで歯周状態は安定していた。一方、#45部の破折部のPPDは深いままであったが、排膿は消失しその後2年3ヵ月まで同症状は認められなかった。以上のことから、SRPと光線力学療法の併用により歯周病が改善し、SPT期における長期的な歯周組織の安定のためには、光線力学療法の応用が重要であることが示唆された。(著者抄録)

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    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2019&ichushi_jid=J00440&link_issn=&doc_id=20190521100005&doc_link_id=%2Fdk1dehea%2F2019%2F006902%2F005%2F0098-0105%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdk1dehea%2F2019%2F006902%2F005%2F0098-0105%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  • 幼児における保育環境とう蝕発生との関係

    横井 彩, 竹内 倫子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   69 ( 増刊 )   205 - 205   2019年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • The level of salivary lactate dehydrogenase as an indicator of the association between gingivitis and related factors in Japanese university students.

    Ayano Taniguchi-Tabata, Daisuke Ekuni, Tetsuji Azuma, Toshiki Yoneda, Mayu Yamane-Takeuchi, Kota Kataoka, Hirofumi Mizuno, Hisataka Miyai, Yoshiaki Iwasaki, Manabu Morita

    Journal of oral science   61 ( 1 )   133 - 139   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The aim of this study was to investigate the association between the presence of gingivitis estimated using the salivary level of lactate dehydrogenase (LD) and related factors in young Japanese adults. Data from 1,915 participants (21.4 ± 2.5 years) were analyzed. Unstimulated saliva was collected from each participant and the salivary LD level was evaluated using a commercially available test kit with an integer scale ranging from 1 to 10. Gingivitis was defined as the LD level of ≥8. The number of permanent teeth, the simplified oral hygiene index (OHI-S), the presence of partially erupted molars and body mass index were recorded. Additionally, participants answered a questionnaire. The percentage of male participants, the number of permanent teeth, the OHI-S and the presence of partially erupted molars were higher, whereas the proportion receiving dental check-ups was lower in the gingivitis group (n = 88, 4.6%) than in the healthy group. Logistic regression analysis showed that gingivitis was significantly associated with OHI-S (OR: 2.68, 95% CI: 1.94-3.69) and receiving dental checkups (OR: 0.31, 95% CI: 0.10-0.99). The present findings indicated that the OHI-S and receiving dental checkups were significantly associated with gingivitis, as assessed by the salivary LD level, in this cohort.

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  • Detection of Salivary miRNAs Reflecting Chronic Periodontitis: A Pilot Study. 国際誌

    Kohei Fujimori, Toshiki Yoneda, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Hirofumi Mizuno, Yoshio Sugiura, Manabu Morita

    Molecules (Basel, Switzerland)   24 ( 6 )   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purpose of this cross-sectional pilot study was to find salivary microRNAs (miRNAs) reflecting periodontal condition in chronic periodontitis. One hundred and twenty chronic periodontitis patients (mean age, 68.4 years) participated in the study, from whom unstimulated whole saliva was collected. A multiphase study was conducted to explore salivary miRNAs as biomarkers of periodontitis. At first, a polymerase chain reaction (PCR) array was performed to compare salivary miRNAs profiles in no and mild (no/mild) and severe periodontitis patients. Next, the relative expression of salivary miRNAs on individual samples was assessed by real-time reverse transcription-PCR. The numbers (%) of patients were 26 (21.6%, no/mild), 58 (48.3%, moderate) and 36 (30.0%, severe), respectively. Among 84 miRNAs, only the relative expression of hsa-miR-381-3p in the severe periodontitis group was significantly higher than that of the no/mild periodontitis group (p < 0.05). Among the 120 patients, there was also a significant correlation between the relative expression of hsa-miR-381-3p and the mean probing pocket depth (PPD) (r = 0.181, p < 0.05). Salivary hsa-miR-381-3p was correlated with periodontitis condition in chronic periodontitis patients.

    DOI: 10.3390/molecules24061034

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  • Detection of Salivary miRNAs Reflecting Chronic Periodontitis: A Pilot Study. 査読 国際誌

    Fujimori K, Yoneda T, Tomofuji T, Ekuni D, Azuma T, Maruyama T, Mizuno H, Sugiura Y, Morita M

    Molecules (Basel, Switzerland)   24 ( 6 )   2019年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/molecules24061034

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  • Awareness of Clenching and Underweight are Risk Factors for Onset of Crowding in Young Adults: A Prospective 3-Year Cohort Study. 国際誌

    Naoki Toyama, Daisuke Ekuni, Ayano Taniguchi-Tabata, Kota Kataoka, Mayu Yamane-Takeuchi, Kohei Fujimori, Terumasa Kobayashi, Daiki Fukuhara, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   16 ( 5 )   2019年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Bruxism is a parafunctional activity that can seriously affect quality of life. Although bruxism induces many problems in the oral and maxillofacial area, whether it contributes to the onset of malocclusion remains unclear. The purpose of this prospective cohort study was to investigate the association between the onset of malocclusion and awareness of clenching during the daytime in young adults. Among 1,092 Okayama University students who underwent normal occlusion at baseline, we analysed 238 who had undergone a dental examination and had complete data after 3 years (2013⁻2016). We also performed subgroup analysis to focus on the association between awake bruxism and the onset of crowding (n = 216). Odds ratios (ORs) were calculated using multivariate logistic regression analyses. The incidences of malocclusion and crowding were 53.8% and 44.5%, respectively. In multivariate logistic regression, awareness of clenching was a risk factor for crowding (OR: 3.63; 95% confidence interval [CI]: 1.08⁻12.17). Moreover, underweight (body mass index < 18.5 kg/m²) was related to the onset of malocclusion (OR: 2.34; 95%CI: 1.11⁻4.92) and crowding (OR: 2.52, 95%CI: 1.25⁻5.76). These results suggest that awareness of clenching during the daytime and underweight are risk factors for the onset of crowding in young adults.

    DOI: 10.3390/ijerph16050690

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  • Relationship Between Renal Dysfunction and Oral Mucositis in Patients Undergoing Concurrent Chemoradiotherapy for Pharyngeal Cancer: A Retrospective Cohort Study. 査読

    Mizuno H, Miyai H, Yokoi A, Kobayashi T, Inabu C, Maruyama T, Ekuni D, Mizukawa N, Kariya S, Nishizaki K, Kimata Y, Morita M

    In vivo (Athens, Greece)   33 ( 1 )   183 - 189   2019年1月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.21873/invivo.11457

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  • Association among secondhand smoke exposure, sleep quality, and prevalence of sleep bruxism in Japanese young adults: A cross-sectional study

    Naoki Toyama, Daisuke Ekuni, Ayano Taniguchi-Tabata, Toshiki Yoneda, Kota Kataoka, Aya Yokoi, Yoko Uchida, Daiki Fukuhara, Hikari Saho, Islam Monirul, Nanami Sawada, Yukiho Nakashima, Yoshiaki Iwasaki, Manabu Morita

    TOBACCO INDUCED DISEASES   17   26 - 27   2019年

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    記述言語:英語   出版者・発行元:EUROPEAN PUBLISHING  

    DOI: 10.18332/tid/111538

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  • Relationship Between Renal Dysfunction and Oral Mucositis in Patients Undergoing Concurrent Chemoradiotherapy for Pharyngeal Cancer: A Retrospective Cohort Study. 国際誌

    Hirofumi Mizuno, Hisataka Miyai, Aya Yokoi, Terumasa Kobayashi, Chiaki Inabu, Takayuki Maruyama, Daisuke Ekuni, Nobuyoshi Mizukawa, Shin Kariya, Kazunori Nishizaki, Yoshihiro Kimata, Manabu Morita

    In vivo (Athens, Greece)   33 ( 1 )   183 - 189   2019年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND/AIM: The aim of this retrospective cohort study was to investigate the association between renal dysfunction (RD) and the development of oral mucositis (OM) in patients undergoing concurrent chemoradiotherapy (CCRT) for pharyngeal cancer including radiation to the oral cavity. PATIENTS AND METHODS: Of 130 patients diagnosed as having pharyngeal cancer who received CCRT at the Okayama University Hospital Head and Neck Cancer Center, 44 were finally selected. RESULTS: During the observation period, 24 (54.5%) patients experienced severe OM (grade 3). The Cox proportional hazards regression model demonstrated that RD (hazard ratio(HR)=2.45, 95% confidence interval(CI)=1.067-6.116, p=0.035) and nasopharynx/oropharynx as center of the irradiated area (HR=2.56, 95% CI=1.072-5.604, p=0.034) were significantly associated with the incidence of severe OM (grade 3). CONCLUSION: In patients with pharyngeal cancer treated with CCRT including radiation to the oral cavity, RD at baseline can be a risk factor for developing severe OM.

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  • New oral hygiene care regimen reduces postoperative oral bacteria count and number of days with elevated fever in ICU patients with esophageal cancer.

    Hirofumi Mizuno, Shinsuke Mizutani, Daisuke Ekuni, Ayano Tabata-Taniguchi, Takayuki Maruyama, Aya Yokoi, Chie Omori, Kazuyoshi Shimizu, Hiroshi Morimatsu, Yasuhiro Shirakawa, Manabu Morita

    Journal of oral science   60 ( 4 )   536 - 543   2018年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Using a controlled pre/post study design, we investigated the effects of professional mechanical cleaning of the oral cavity with benzethonium chloride, interdental brushes, and hydrogen peroxide on the number of oral bacteria and postoperative complications among esophageal cancer patients in an intensive care unit. Before surgery, 44 patients with esophageal cancer were recruited at Okayama Hospital from January through August 2015. The control group (n = 23) received routine oral hygiene care in the intensive care unit. The intervention group (n = 21) received intensive interdental cleaning with benzethonium chloride solution and tongue cleaning with hydrogen peroxide. The number of oral bacteria on the tongue surface and plaque index were significantly lower in the intervention group than in the control group on postoperative days 1 and 2 (P < 0.05). Additionally, the number of days with elevated fever during a 1-week period was significantly lower in the intervention group than in the control group (P = 0.037). As compared with routine oral hygiene, a new oral hygiene regimen comprising benzethonium chloride, interdental brushes, and hydrogen peroxide significantly reduced the number of oral bacteria and days with elevated fever in patients with esophageal cancer.

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  • Does Instruction of Oral Health Behavior for Workers Improve Work Performance?-Quasi-Randomized Trial. 国際誌

    Naoki Toyama, Ayano Taniguchi-Tabata, Nanami Sawada, Yoshio Sugiura, Daiki Fukuhara, Yoko Uchida, Hisataka Miyai, Aya Yokoi, Shinsuke Mizutani, Daisuke Ekuni, Manabu Morita

    International journal of environmental research and public health   15 ( 12 )   2018年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Oral disease can cause economic loss due to impaired work performance. Therefore, improvement of oral health status and prevention of oral disease is essential among workers. The purpose of this study was to investigate whether oral health-related behavioral modification intervention influences work performance or improves oral health behavior and oral health status among Japanese workers. We quasi-randomly separated participants into the intervention group or the control group at baseline. The intervention group received intensive oral health instruction at baseline and a self-assessment every three months. Both groups received oral examinations and answered the self-questionnaire at baseline and at one-year follow-up. At follow-up, the prevalence of subjects who use fluoride toothpastes and interdental brushes/dental floss were significantly higher in the intervention group than in the control group. Three variables (tooth brushing in workplace, using fluoride toothpaste, and experience of receiving tooth brushing instruction in a dental clinic) showed significant improvement only in the intervention group. On the other hand, work performance and oral status did not significantly change in either group. Our intensive oral health-related behavioral modification intervention improved oral health behavior, but neither work performance nor oral status, among Japanese workers.

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  • 嚥下機能はオーラルディアドコキネシスと関連する

    竹内 倫子, 江國 大輔, 森田 学

    日本公衆衛生学会総会抄録集   77回   243 - 243   2018年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • Impact of commensal flora on periodontal immune response to lipopolysaccharide. 査読 国際誌

    Fukuhara D, Irie K, Uchida Y, Kataoka K, Akiyama K, Ekuni D, Tomofuji T, Morita M

    Journal of periodontology   89 ( 10 )   1213 - 1220   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/JPER.17-0567

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  • Impact of commensal flora on periodontal immune response to lipopolysaccharide. 国際誌

    Daiki Fukuhara, Koichiro Irie, Yoko Uchida, Kota Kataoka, Kentaro Akiyama, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    Journal of periodontology   89 ( 10 )   1213 - 1220   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Commensal flora are involved in the appropriate development of the mature immune system. However, it is unclear how commensal flora contribute to immune responses against periodontal pathogens, including the response to lipopolysaccharide (LPS). The purpose of this study was to evaluate the expression of immune responses after topical application of LPS in germ-free (GF) and specific-pathogen-free (SPF) mice. METHODS: GF and SPF mice at 8 weeks of age were randomly divided into four groups each: a baseline group (n = 4/group) and three experimental groups (n = 6/group). Experimental groups received topical application of Porphyromonas gingivalis LPS (10 μg/μL) into the palatal gingival sulcus. Sampling was performed before LPS application (baseline) and at 3, 24, or 72 hours after LPS application. The numbers of neutrophils, CD4+ , and CD8+ T cells in periodontal tissue were evaluated by immunohistochemistry. Expression of genes encoding cytokines, chemokines, and a transcription factor was determined by real-time PCR. RESULTS: SPF mice, but not GF mice, showed an increased number of CD4+ T cells in the periodontal tissue at 3 hours after LPS application, compared with the number at baseline (p < 0.05). Gene expressions of tumor necrosis factor-α (Tnf-α) and forkhead box protein p3 (Foxp3) was also significantly higher in the SPF mice than in the GF mice at 3 hours after LPS application (p < 0.05). The number of neutrophils peaked at 24 hours in both GF and SPF mice. CONCLUSIONS: LPS-exposed SPF mice exhibited increases in the number of CD4+ T cells and in Tnf-α and Foxp3 gene expression in periodontal tissue compared with LPS-exposed GF mice.

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  • Unusual oral mucosal microbiota after hematopoietic cell transplantation with glycopeptide antibiotics: potential association with pathophysiology of oral mucositis. 国際誌

    Misato Muro, Yoshihiko Soga, Tomoko Higuchi, Kota Kataoka, Daisuke Ekuni, Yoshinobu Maeda, Manabu Morita

    Folia microbiologica   63 ( 5 )   587 - 597   2018年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Severe oral mucositis occurs frequently in patients receiving hematopoietic stem cell transplantation (HCT). Oral mucosal bacteria can be associated with progression of oral mucositis, and systemic infection may occur via ulcerative oral mucositis. However, little information is available regarding the oral microbiota after HCT. Here, PCR-denaturing gradient gel electrophoresis (DGGE) was performed to characterize the oral mucosal microbiota, which can be affected by antibiotics, before and after HCT. Sixty reduced-intensity HCT patients were enrolled. Three patients with the least antibiotic use (quinolone prophylaxis and/or β-lactam monotherapy group) and three patients with the most antibiotic use (β-lactam-glycopeptide combination therapy group) were selected. Bacterial DNA samples obtained from the oral mucosa before and after HCT were subjected to PCR-DGGE. The trajectory of oral mucositis was evaluated. The oral mucosal microbiota in the β-lactam-glycopeptide combination therapy group was different from that in the quinolone prophylaxis and/or β-lactam monotherapy group, and Staphylococcus spp. and Enterococcus spp. were identified. Lautropia mirabilis was dominant in one patient. Ulcerative oral mucositis was observed only in the β-lactam-glycopeptide combination therapy group. In conclusion, especially with the use of strong antibiotics, such as glycopeptides, the oral mucosal microbiota differed completely from that under normal conditions and consisted of Staphylococcus spp., Enterococcus spp., and unexpectedly L. mirabilis. The normal oral microbiota consists not only of bacteria, but these unexpected bacteria could be involved in the pathophysiology as well as systemic infection via oral mucositis. Our results can be used as the basis for future studies in larger patient populations.

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  • New oral hygiene care regimen reduces postoperative oral bacteria count and number of days with elevated fever in ICU patients with esophageal cancer. 査読

    Mizuno H, Mizutani S, Ekuni D, Tabata-Taniguchi A, Maruyama T, Yokoi A, Omori C, Shimizu K, Morimatsu H, Shirakawa Y, Morita M

    Journal of oral science   60 ( 4 )   536 - 543   2018年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2334/josnusd.17-0381

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  • Commensal Microbiota Enhance Both Osteoclast and Osteoblast Activities. 国際誌

    Yoko Uchida, Koichiro Irie, Daiki Fukuhara, Kota Kataoka, Takako Hattori, Mitsuaki Ono, Daisuke Ekuni, Satoshi Kubota, Manabu Morita

    Molecules (Basel, Switzerland)   23 ( 7 )   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Recent studies suggest that the commensal microbiota affects not only host energy metabolism and development of immunity but also bone remodeling by positive regulation of osteoclast activity. However, the mechanism of regulation of bone cells by the commensal microbiota has not been elucidated. In this study, 8-week-old specific pathogen-free (SPF) and germ-free (GF) mice were compared in terms of alveolar bones and primary osteoblasts isolated from calvarias. Micro-CT analysis showed that SPF mice had larger body size associated with lower bone mineral density and bone volume fraction in alveolar bones compared with GF mice. Greater numbers of osteoclasts in alveolar bone and higher serum levels of tartrate-resistant acid phosphatase 5b were observed in SPF mice. Tissue extracts from SPF alveolar bone showed higher levels of cathepsin K, indicating higher osteoclast activity. SPF alveolar extracts also showed elevated levels of γ-carboxylated glutamic acid⁻osteocalcin as a marker of mature osteoblasts compared with GF mice. Polymerase chain reaction (PCR) array analysis of RNA directly isolated from alveolar bone showed that in SPF mice, expression of mRNA of osteocalcin, which also acts as an inhibitor of bone mineralization, was strongly enhanced compared with GF mice. Cultured calvarial osteoblasts from SPF mice showed reduced mineralization but significantly enhanced expression of mRNAs of osteocalcin, alkaline phosphatase, insulin-like growth factor-I/II, and decreased ratio of osteoprotegerin/receptor activator of nuclear factor-kappa B ligand compared with GF mice. Furthermore, PCR array analyses of transcription factors in cultured calvarial osteoblasts showed strongly upregulated expression of Forkhead box g1. In contrast, Gata-binding protein 3 was strongly downregulated in SPF osteoblasts. These results suggest that the commensal microbiota prevents excessive mineralization possibly by stimulating osteocalcin expression in osteoblasts, and enhances both osteoblast and osteoclast activity by regulating specific transcription factors.

    DOI: 10.3390/molecules23071517

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  • Association between intensive health guidance focusing on eating quickly and metabolic syndrome in Japanese middle-aged citizens 査読 国際誌

    Daisuke Ekuni, Michiko Furuta, Toshihide Kimura, Naoki Toyama, Daiki Fukuhara, Yoko Uchida, Nanami Sawada, Manabu Morita

    Eating and Weight Disorders   25 ( 1 )   1 - 8   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer International Publishing  

    Purpose: The purpose of this intervention study was to investigate whether intensive health guidance focusing on eating quickly can prevent metabolic syndrome (MetS) more effectively than standard routine guidance in Japanese citizens living in rural areas. Methods: This controlled, non-randomized, intervention study analyzed 141 participants with MetS at baseline. Participants in the intervention group received health guidance focusing on eating quickly and standard health guidance about MetS in accordance with the guidelines of the Ministry of Health, Labour and Welfare in Japan, whereas participants in the control group received only standard health guidance about MetS. The primary study outcome was the prevalence of MetS at a 1-year follow-up. Results: At 1-year follow-up, the prevalence of MetS in the intervention group was significantly lower than that in the control group (p = 0.003). The decreases in body weight, body mass index, waist circumference and triglycerides from baseline to 1 year were significantly greater in the intervention group than in the control group (p &lt
    0.05). Conclusion: Intensive health guidance focusing on eating quickly is more effective for improving MetS than standard Japanese health guidance alone. Level of evidence: Level II, Evidence obtained from well-designed controlled trials without randomization. Trial registry name, registration identification number, and URL for the registry: UMIN, UMIN000030600, http://www.umin.ac.jp/ctr/index-j.htm.

    DOI: 10.1007/s40519-018-0522-1

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  • Relationship between severity of periodontitis and masseter muscle activity during waking and sleeping hours 査読 国際誌

    Seiya Kato, Daisuke Ekuni, Shigehisa Kawakami, Acing Habibie Mude, Manabu Morita, Shogo Minagi

    Archives of Oral Biology   90   13 - 18   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Ltd  

    Objective: This study was conducted to investigate whether the masseter muscle activity shows any specific pattern in relation to the severity of periodontitis. Design: Sixteen subjects with no or mild periodontitis (NMP group) and 15 subjects with moderate or severe periodontitis (MSP group) were enrolled. Plasma IgG antibody titer was examined using Porphyromonas gingivalis as a bacterial antigen. Surface electromyography (EMG) of the masseter muscles was continuously recorded using an ambulatory surface EMG recording device while patients were awake and asleep. Masseter muscle activity was analyzed using intensities of 5%–10% maximal voluntary clenching (MVC), 10%–20% MVC, and &gt
    20% MVC. Furthermore, EMG levels of 20% MVC were adopted as the threshold for analysis of phasic, tonic, and mixed EMG activities. The cumulative duration of masseter muscle activity and bruxism episodes was calculated as duration per hour. Results: There was no significant difference in plasma IgG antibody titers against P. gingivalis between the NMP and MSP groups (p = 0.423). During waking hours, the duration of masseter muscle activity with an intensity of &gt
    20% MVC was significantly longer in the MSP group than in the NMP group (p = 0.037). During sleeping hours, the duration of masseter muscle activity at all MVC intensities was significantly longer in the MSP group than in the NMP group (all p &lt
    0.05). Additionally, the duration of phasic and mixed episodes was significantly longer in the MSP group than those in the NMP group while both awake and asleep (all p &lt
    0.05). Conclusions: The results of this study suggested that masseter muscle activity might be related to the severity of periodontitis.

    DOI: 10.1016/j.archoralbio.2018.02.021

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  • Commensal Microbiota Enhance Both Osteoclast and Osteoblast Activities. 査読 国際誌

    Uchida Y, Irie K, Fukuhara D, Kataoka K, Hattori T, Ono M, Ekuni D, Kubota S, Morita M

    Molecules (Basel, Switzerland)   23 ( 7 )   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/molecules23071517

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  • Relationship between severity of periodontitis and masseter muscle activity during waking and sleeping hours. 国際誌

    Seiya Kato, Daisuke Ekuni, Shigehisa Kawakami, Acing Habibie Mude, Manabu Morita, Shogo Minagi

    Archives of oral biology   90   13 - 18   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: This study was conducted to investigate whether the masseter muscle activity shows any specific pattern in relation to the severity of periodontitis. DESIGN: Sixteen subjects with no or mild periodontitis (NMP group) and 15 subjects with moderate or severe periodontitis (MSP group) were enrolled. Plasma IgG antibody titer was examined using Porphyromonas gingivalis as a bacterial antigen. Surface electromyography (EMG) of the masseter muscles was continuously recorded using an ambulatory surface EMG recording device while patients were awake and asleep. Masseter muscle activity was analyzed using intensities of 5%-10% maximal voluntary clenching (MVC), 10%-20% MVC, and >20% MVC. Furthermore, EMG levels of 20% MVC were adopted as the threshold for analysis of phasic, tonic, and mixed EMG activities. The cumulative duration of masseter muscle activity and bruxism episodes was calculated as duration per hour. RESULTS: There was no significant difference in plasma IgG antibody titers against P. gingivalis between the NMP and MSP groups (p = 0.423). During waking hours, the duration of masseter muscle activity with an intensity of >20% MVC was significantly longer in the MSP group than in the NMP group (p = 0.037). During sleeping hours, the duration of masseter muscle activity at all MVC intensities was significantly longer in the MSP group than in the NMP group (all p < 0.05). Additionally, the duration of phasic and mixed episodes was significantly longer in the MSP group than those in the NMP group while both awake and asleep (all p < 0.05). CONCLUSIONS: The results of this study suggested that masseter muscle activity might be related to the severity of periodontitis.

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  • Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium 査読 国際誌

    Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Daiki Fukuhara, Yoko Uchida, Kota Kataoka, Shuichiro Kobayashi, Takeshi Kikuchi, Akio Mitani, Yoshihiro Shimazaki, Manabu Morita

    Archives of Oral Biology   87   72 - 78   2018年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Ltd  

    Objective Increasing age is a potential risk factor for periodontal tissue breakdown, which may be affected by commensal flora. The aim of this study evaluated age-related changes in CD4+ T cells, C-C chemokine ligand 5 (CCL5), interleukin (IL)-17A, and receptor activator of nuclear factor-kappa B ligand (RANKL) expression using germ-free (GF) and conventionally reared (SPF) mice. Design GF and SPF mice at 8 (n = 6/group) and 22 weeks old (n = 6/group) were used. Immunohistochemical analyses were performed to determine the effects of aging on protein expression in periodontal tissues. Age-related changes in alveolar bone were quantified using micro-CT analysis. Results SPF mice, but not GF mice, showed an age-related increase in alveolar bone loss (P &lt
    0.01). SPF mice at 22 weeks of age increased expression of CD4+ T cells, CCL5, IL-17A, and RANKL compared to those at 8 weeks of age in connective tissue and alveolar bone surface (P &lt
    0.01). Furthermore, there was increased CD4+ T cells, which were co-expressed with IL-17A and RANKL in SPF mice at 22 weeks of age. On the other hand, the GF mice did not show any significant differences in CD4+ T cells, CCL5, IL-17A and RANKL expression between the two age groups. Conclusions SPF mice induced an age-related increase in CD4+ T cells co- expressed with IL-17A and RANKL, with occurring alveolar bone loss. In contrast, GF mice did not show age-related changes in CD4+ T cell migration and cytokine expression.

    DOI: 10.1016/j.archoralbio.2017.12.007

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  • Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium. 国際誌

    Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Daiki Fukuhara, Yoko Uchida, Kota Kataoka, Shuichiro Kobayashi, Takeshi Kikuchi, Akio Mitani, Yoshihiro Shimazaki, Manabu Morita

    Archives of oral biology   87   72 - 78   2018年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: Increasing age is a potential risk factor for periodontal tissue breakdown, which may be affected by commensal flora. The aim of this study evaluated age-related changes in CD4+ T cells, C-C chemokine ligand 5 (CCL5), interleukin (IL)-17A, and receptor activator of nuclear factor-kappa B ligand (RANKL) expression using germ-free (GF) and conventionally reared (SPF) mice. DESIGN: GF and SPF mice at 8 (n = 6/group) and 22 weeks old (n = 6/group) were used. Immunohistochemical analyses were performed to determine the effects of aging on protein expression in periodontal tissues. Age-related changes in alveolar bone were quantified using micro-CT analysis. RESULTS: SPF mice, but not GF mice, showed an age-related increase in alveolar bone loss (P < 0.01). SPF mice at 22 weeks of age increased expression of CD4+ T cells, CCL5, IL-17A, and RANKL compared to those at 8 weeks of age in connective tissue and alveolar bone surface (P < 0.01). Furthermore, there was increased CD4+ T cells, which were co-expressed with IL-17A and RANKL in SPF mice at 22 weeks of age. On the other hand, the GF mice did not show any significant differences in CD4+ T cells, CCL5, IL-17A and RANKL expression between the two age groups. CONCLUSIONS: SPF mice induced an age-related increase in CD4+ T cells co- expressed with IL-17A and RANKL, with occurring alveolar bone loss. In contrast, GF mice did not show age-related changes in CD4+ T cell migration and cytokine expression.

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  • Topical application of ointment containing 0.5% green tea catechins suppresses tongue oxidative stress in 5-fluorouracil administered rats. 国際誌

    Hisataka Miyai, Takayuki Maruyama, Takaaki Tomofuji, Toshiki Yoneda, Tetsuji Azuma, Hirofumi Mizuno, Yoshio Sugiura, Terumasa Kobayashi, Daisuke Ekuni, Manabu Morita

    Archives of oral biology   82   247 - 255   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats. DESIGN: Male Wistar rats (n=28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1% or 0.5% green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus. RESULTS: After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P<0.05). On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group (P<0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5% green tea catechin group than in the positive control group (P<0.05). CONCLUSIONS: Topical application of ointment containing 0.5% green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.

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  • 職域における早食いとメタボリックシンドロームとの関連

    横井 彩, 江國 大輔, 米田 俊樹, 森田 学

    口腔衛生学会雑誌   67 ( 3 )   202 - 202   2017年7月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 常在菌が骨芽細胞に与える影響について

    内田 瑶子, 入江 浩一郎, 福原 大樹, 片岡 広太, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   67 ( 増刊 )   106 - 106   2017年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • LPSを塗布した歯周組織の免疫応答に対する常在菌の影響

    福原 大樹, 入江 浩一郎, 内田 瑶子, 片岡 広太, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   67 ( 増刊 )   108 - 108   2017年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • The effects of non-surgical periodontal treatment on glycemic control, oxidative stress balance and quality of life in patients with type 2 diabetes: A randomized clinical trial. 国際誌

    Hirofumi Mizuno, Daisuke Ekuni, Takayuki Maruyama, Kota Kataoka, Toshiki Yoneda, Daiki Fukuhara, Yoshio Sugiura, Takaaki Tomofuji, Jun Wada, Manabu Morita

    PloS one   12 ( 11 )   e0188171   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    AIM: The purpose of this study was to investigate the effects of non-surgical periodontal treatment on hemoglobinA1c (HbA1c) levels, oxidative stress balance and quality of life (QOL) in patients with type 2 diabetes mellitus (T2DM) compared to no periodontal treatment (simple oral hygiene instructions only). METHODS: The design was a 6-month, single-masked, single center, randomized clinical trial. Patients had both T2DM and chronic periodontitis. Forty participants were enrolled between April 2014 and March 2016 at the Nephrology, Diabetology and Endocrinology Department of Okayama University Hospital. The periodontal treatment group (n = 20) received non-surgical periodontal therapy, including scaling and root planing plus oral hygiene instructions, and consecutive supportive periodontal therapy at 3 and 6 months. The control group (n = 17) received only oral hygiene instructions without treatment during the experimental period. The primary study outcome was the change in HbA1c levels from baseline to 3 months. Secondary outcomes included changes in oxidative stress balance (Oxidative-INDEX), the Diabetes Therapy-Related QOL and clinical periodontal parameters from baseline to 3 months and baseline to 6 months. RESULTS: Changes in HbA1c in the periodontal treatment group were not significantly different with those in the control group at 3 and 6 months. Systemic oxidative stress balance and QOL significantly improved in the periodontal treatment group compared to the control group at 3 months. In the subgroup analysis (moderately poor control of diabetes), the decrease in HbA1c levels in the periodontal treatment group was greater than that in the control group at 3 months but not significant. CONCLUSIONS: In T2DM patients, non-surgical periodontal treatment improved systemic oxidative stress balance and QOL, but did not decrease HbA1c levels at 3 months follow-up. TRIAL REGISTRATION: Current Controlled Trials UMIN-ICDR UMIN 000013278 (Registered April 1, 2014).

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  • 高校生におけるう蝕発症と口腔保健行動との関連性

    谷口 綾乃, 竹内 倫子, 江國 大輔, 森田 学

    日本公衆衛生学会総会抄録集   75回   474 - 474   2016年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • 早食いに関する保健指導はメタボリックシンドロームを減少させる

    江國 大輔, 古田 美智子, 友藤 孝明, 外山 直樹, 谷口 綾乃, 竹内 真由, 竹内 倫子, 森田 学

    日本公衆衛生学会総会抄録集   75回   556 - 556   2016年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • 職域における職業階層、労働形態および歯周病罹患との関連

    外山 直樹, 入江 浩一郎, 谷口 綾乃, 江國 大輔, 友藤 孝明, 森田 学

    日本公衆衛生学会総会抄録集   75回   651 - 651   2016年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • 歯周組織の加齢変化におけるCD4+ T細胞の影響

    福原 大樹, 入江 浩一郎, 内田 瑶子, 片岡 広太, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   66 ( 2 )   286 - 286   2016年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 口腔常在菌が歯槽骨に与える影響について

    内田 瑶子, 入江 浩一郎, 福原 大樹, 片岡 広太, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   66 ( 2 )   213 - 213   2016年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 妊婦の歯周状態と胎児発育との関係 酸化ストレスを切り口にした検討

    竹内 倫子, 佐久本 雅美, 江國 大輔, 友藤 孝明, 森田 学

    日本公衆衛生学会総会抄録集   74回   321 - 321   2015年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • 歯周組織の加齢変化に対する口腔常在菌の影響

    入江 浩一郎, 友藤 孝明, 江國 大輔, 小林 周一郎, 菊池 毅, 内田 瑶子, 福原 大樹, 三谷 章雄, 嶋崎 義浩, 森田 学

    日本歯周病学会会誌   57 ( 秋季特別 )   131 - 131   2015年8月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯科矯正治療の前後における咀嚼能力とストレスの変化

    大森 智栄, 江國 大輔, 竹内 倫子, 山根 真由, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   65 ( 2 )   252 - 252   2015年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 動脈硬化への咬合異常の影響

    江國 大輔

    上原記念生命科学財団研究報告集   28   1 - 4   2014年12月

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    記述言語:日本語   出版者・発行元:(公財)上原記念生命科学財団  

    咬合異常によるアテローム性動脈硬化への影響をアポリポ蛋白E欠損ラット(動脈硬化モデル)を用いて検討した。対照群には処置はせず、咬含異常群では左右の上顎臼歯すべての咬頭を削合して、8週間の通常飼育を行った。血漿中のコルチコステロン濃度は、咬合異常群において対照群よりも有意に高い値を示した。また、対照群と咬合異常群の間において、血液中のVLDL濃度・LDL濃度、動脈のプラークの付着面積・脂肪沈着面積・VCAM1の発現・VCAM1の遺伝子発現・TLR4の遺伝子発現に有意な差があった。血清中のHDL、ox-LDL、ROM、CRPでは2群間に有意差は見られなかった。

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  • 岡山大学病院口臭外来初診患者の実態調査

    森田 学, 貞森 なみ子, 竹内 倫子, 江國 大輔

    日本口臭学会会誌   5 ( 1 )   40 - 40   2014年6月

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  • Longitudinal relationship between plasma reactive oxygen metabolites and periodontal condition in the maintenance phase of periodontal treatment. 査読 国際誌

    Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Mayu Yamane, Toshiki Yoneda, Yuya Kawabata, Kota Kataoka, Naofumi Tamaki, Manabu Morita

    Disease Markers   Vol.2014   489292 - 489292   2014年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    When plasma ROM level in periodontitis patients was low, increases in plasma ROM level were associated with those in CAL during the maintenance phase of treatment.

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  • アルコール摂取後のブラッシング行動と歯周病の活動性との関連

    水谷 慎介, 江國 大輔, 友藤 孝明, 山根 真由, 東 哲司, 丸山 貴之, 町田 達哉, 米田 俊樹, 片岡 広太, 佐久本 雅美, 川端 勇也, 大森 智栄, 森田 学

    口腔衛生学会雑誌   64 ( 2 )   173 - 173   2014年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • Anti-ageing effects of dentifrices containing antioxidative, anti-inflammatory, and anti-bacterial agents (Tomarina) on gingival collagen degradation in rats 査読 国際誌

    irie Koichiro, Takaaki Tomofuji, Ekuni Daisuke, Endo Yasumasa, Kasuyama Kenta, Tetsuji Azuma, Naofumi Tamaki, Yoneda Toshiki, Morita Manabu

    Archives of Oral Biology   Vol.59 ( No.1 )   60 - 65   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Previous studies have demonstrated the relationship between ageing and oxidative stress. In this study, we examined the effects of topical application of a dentifrice containing anti-oxidative, anti-inflammatory, and anti-bacterial agents (Tomarina?) to the gingival surface on gingival collagen degradation in rats. Fischer 344 male rats (4 or 8 months old) were divided into two groups: experimental group and control group. Tomarina? (the experimental group) or control dentifrice (the control group) was applied 5 days per week for 2 months. In the control group, gingival collagen density decreased with ageing. In the experimental group, the collagen density did not change with ageing, and was greater than that in the control group at 10 months of age (p &lt; 0.0083). In addition, the control group showed an increase in serum oxidative stress with ageing. The experimental group also showed increased serum oxidative stress, but the value was lower than the control group at 10 months of age (p &lt; 0.0083). Furthermore, low expressions of protein oxidative damage in the periodontal tissue were observed in the experimental group, compared to the control group at 6 months and 10 months. These findings indicate that Tomarina? might suppress the effects of ageing on gingival collagen degradation, by decreasing oxidative stress in the rat model.

    DOI: 10.1016/j.archoralbio.2013.09.008

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  • 高校生を対象とした歯種別う蝕罹患の追跡調査

    竹内 倫子, 友藤 孝明, 江國 大輔, 森田 学

    日本公衆衛生学会総会抄録集   72回   370 - 370   2013年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • 岡山市内3小学校の児童における永久歯の萠出状況

    横井 彩, 竹内 倫子, 江國 大輔, 友藤 孝明, 町田 達哉, 森田 学

    口腔衛生学会雑誌   63 ( 3 )   290 - 291   2013年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 歯科医師による歯磨きが状態不安と特性不安の程度に及ぼす影響

    友藤 孝明, 町田 達哉, 竹内 倫子, 江國 大輔, 森田 学

    日本公衆衛生学会総会抄録集   71回   449 - 449   2012年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • 早食いの是正に対するパンフレットと食行動記録法の効果

    竹内 倫子, 江國 大輔, 友藤 孝明, 古田 美智子, 森田 学

    口腔衛生学会雑誌   62 ( 4 )   384 - 390   2012年7月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

    早食いを自覚する者に対して、早食い防止啓発パンフレットの配布、あるいはパンフレットの配布と食行動記録の指示を行い、それぞれの効果を比較検討することを目的とした。岡山大学歯学部学生115人の中から、アンケート調査で早食いを自覚している者43名を選出し、早食い防止啓発パンフレットを配布した群(パンフレット群)とパンフレットを配布し、さらに食行動記録を指示した群(パンフレット+食行動記録群)にランダムに振り分けた。食行動については、アンケートによる主観的評価のほか、鮭おにぎりを完食するまでの総咀嚼時間、総咀嚼回数、口に運ぶ回数を客観的に測定し、一口あたりの量および咀嚼速度を算出した。なお、これらの評価は、ベースライン時とその2週間後に行った。その結果、パンフレット+食行動記録群においては、ベースライン時と比較して2週間後では早食いと自覚する者が有意に減少していた。また、2週間後では、「お腹一杯になるまで食べますか」の項目において「いいえ」と答えた者が、パンフレット群よりもパンフレット+食行動記録群で有意に多かった。ベースライン時と2週間後の比較では、介入前のおにぎりの一口あたりの量を1.0とした場合、介入後のおにぎりの一口あたりの量は、パンフレット+食行動記録群、パンフレット群それぞれ0.82と1.00であり、パンフレット+食行動記録群のほうが有意に減少していた。以上のことより、早食い防止啓発パンフレットの配布単独よりも、食行動記録をこれに加えるほうがより早食いの改善に有効かもしれない。(著者抄録)

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    その他リンク: https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2012&ichushi_jid=J00440&link_issn=&doc_id=20120809250005&doc_link_id=%2Fdk1dehea%2F2012%2F006204%2F005%2F0384-0390%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdk1dehea%2F2012%2F006204%2F005%2F0384-0390%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  • Vitamin C: Its antioxidant effects on periodontitis

    Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    Vitamin C: Nutrition, Side Effects and Supplements   311 - 319   2011年1月

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    掲載種別:論文集(書籍)内論文  

    Oxidative stress is involved in the pathogenesis of periodontitis. The effects of a variety of antioxidants onthe prevention and treatment of periodontitis have been examined in animal studies. Vitamin C is a well-known water-soluble vitamin having antioxidant, anticarcinogenic, and immunomodulatory actions. Epidemiological studies have indicated a negative association between plasma vitamin C levels and the severity of periodontitis. A clinical study reports that the intake of grapefruit leads to an increase in plasma vitamin C levels and reduces gingival bleeding. Recent animal studies show that vitamin C intake improves oxidative damage of periodontal tissueand increases the level of serum vitamin C. It is possible that an adequate level of vitamin C intake may function as an antioxidant therapy to control oxidative stress, and be clinically beneficial in suppressingthe progression of human periodontitis. However, large scale randomized clinical trials will be required to confirm the effects of vitamin C on human periodontitis. © 2011 by Nova Science Publishers, Inc. All rights reserved.

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  • 大学生における不正咬合とストレスの関係

    江國 大輔, 古田 美智子, 入江 浩一郎, 東 哲司, 友藤 孝明, 村上 隆, 山城 隆, 加藤 朋, 山田 小百合, 小倉 俊夫, 森田 学

    口腔衛生学会雑誌   60 ( 5 )   606 - 606   2010年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • Supplementation of green tea catechins in dentifrices suppresses gingival oxidative stress and periodontal inflammation. 査読 国際誌

    Takayuki Maruyama, Takaaki Tomofuji, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Daisuke Ekuni, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    Archives of Oral Biology   Vol.56 ( No.1 )   48 - 53   2010年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    adding green tea catechins to a dentifrice may contribute to prevention of periodontal inflammation by decreasing gingival oxidative stress and expression of pro-inflammatory cytokines.

    DOI: 10.1016/j.archoralbio.2010.08.015

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  • 頭頸部癌患者に対する歯科衛生士関わり

    志茂 加代子, 岡崎 惠子, 羽川 操, 江国 大輔, 山中 玲子, 水川 展吉

    岡山歯学会雑誌   29 ( 1 )   72 - 72   2010年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • メインテナンス期の歯周病患者における咬合力と歯周病進行との関係について

    竹内 倫子, 江國 大輔, 山本 龍生, 森田 学

    岡山歯学会雑誌   29 ( 1 )   41 - 49   2010年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

    歯周治療のメインテナンス期にある患者194名を対象に、咬合力と歯周病進行との関係について約3年間にわたり調査し、咬合力が歯周病進行の予測因子になりうるか検討した。その結果、調査期間中に歯周病が進行した群は進行しなかった群に比べて咬合力が有意に弱かったことから、咬合力は歯周病進行の予測因子になりうると考えられた。また、歯周病が進行した群では、調査開始時にクリニカルアタッチメントレベル(CAL)の最大値7mm以上の歯を1本以上有していた患者の割合が有意に高かったことから、「CAL最大値7mm以上」も歯周病進行の予測因子になりうると考えられた。

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  • Experimental periodontitis induces gene expression of proinflammatory cytokines in liver and white adipose tissues in obesity. 査読 国際誌

    Yasumasa Endo, Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    Journal of Periodontology   Vol.81 ( No.4 )   520 - 526   2010年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Systemic low-grade inflammation after experimental periodontitis was associated with increased gene expression for hepatic levels of TNF-alpha and CRP and adipose tissue levels of IL-6 and CRP in the obese-rat model.

    DOI: 10.1902/jop.2009.090574

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  • Effects of periodontitis on aortic insulin resistance in an obese rat model. 査読 国際誌

    Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Tetsuji Azuma, Naofumi Tamaki, Toshihiro Sanbe, Yasumasa Endo, Tatsuo Yamamoto, Takashi Nishida, Manabu Morita

    Laboratory Investigation; a Journal of Technical Methods and Pathology   Vol.90 ( No.3 )   348 - 359   2010年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The combination of obesity and its associated risk factors, such as insulin resistance and inflammation, results in the development of atherosclerosis. However, the effects of periodontitis on atherosclerosis in an obese body remain unclear. The aim of the study was to investigate the effects of ligature-induced periodontitis in Zucker fatty rats on initiation of atherosclerosis by evaluating aortic insulin resistance. Zucker fatty rats (n=24) were divided into two groups. In the periodontitis group, periodontitis was ligature-induced for 4 weeks, whereas the control group was left unligated. After the 4-week experimental period, descending aorta was used for measuring the levels of lipid deposits, immunohistochemical analysis, and evaluation of gene expression. Levels of serum C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-alpha), and insulin were also measured. Rats in the periodontitis group had significantly enhanced lipid deposits in the aorta, but not in the control group. Expression of suppressor of cytokine signaling 3, vascular cell adhesion molecule 1, reactive oxygen species, nitrotyrosine, and endothelin-1 in the periodontitis group was more intense than that in the control group. Significantly decreased levels of phosphatidylinositol 3-kinase (Pi3k) catalytic beta-polypeptide (Pi3kcb), Pi3kp85, and insulin receptor substrate 1 and 2 were observed in the periodontitis group. Levels of serum CRP and TNF-alpha were significantly increased in the periodontitis group. Under insulin-stimulated conditions, aorta in the periodontitis group altered the Akt phosphorylation. Periodontitis in obesity induced the initial stage of atherosclerosis and disturbed aortic insulin signaling.

    DOI: 10.1038/labinvest.2009.141

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  • Relationship between periodontitis and hepatic abnormalities in young adults. 査読 国際誌

    Furuta M, Ekuni D, Yamamoto T, Irie K, Koyama R, Sanbe T, Yamanaka R, Morita M, Kuroki K, Tobe K

    Acta odontologica Scandinavica   68 ( 1 )   27 - 33   2010年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:1  

    DOI: 10.3109/00016350903291913

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  • Imbalance of oxidative/anti-oxidative status induced by periodontitis is involved in apoptosis of rat submandibular glands. 査読 国際誌

    Daisuke Ekuni, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takaaki Tomofuji, Manabu Morita

    Archives of Oral Biology   Vol.55 ( No.2 )   170 - 176   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Imbalance of circulating oxidative/anti-oxidative status may be involved in vacuolisation and apoptosis of submandibular glands in the rat periodontitis model.

    DOI: 10.1016/j.archoralbio.2009.11.013

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  • Preventive effects of a cocoa-enriched diet on gingival oxidative stress in experimental periodontitis. 査読 国際誌

    Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Yasumasa Endo, Naofumi Tamaki, Toshihiro Sanbe, Jun Murakami, Tatsuo Yamamoto, Manabu Morita

    Journal of Periodontology   Vol.80 ( No.11 )   1799 - 1808   2009年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Consuming a cocoa-enriched diet could diminish periodontitis-induced oxidative stress, which, in turn, might suppress the progression of periodontitis.

    DOI: 10.1902/jop.2009.090270

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  • Modified IOTNを用いた不正咬合の基準設定 pilot study

    村上 隆, 江國 大輔, 古田 美智子, 山城 隆

    日本矯正歯科学会大会プログラム・抄録集   68回   152 - 152   2009年11月

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    記述言語:日本語   出版者・発行元:(公社)日本矯正歯科学会  

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  • メインテナンス期の歯周病患者における咬合力と歯周状態の関係について

    竹内 倫子, 山本 龍生, 江國 大輔, 森田 学

    口腔衛生学会雑誌   59 ( 5 )   609 - 609   2009年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • メインテナンス期の歯周病患者において咬合力は予後因子となりうる

    竹内 倫子, 山本 龍生, 江國 大輔, 森田 学

    口腔衛生学会雑誌   59 ( 4 )   327 - 327   2009年8月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 岡山大学病院における頭頸部癌チーム医療と予防歯科の関わり

    山中 玲子, 水川 展吉, 山本 龍生, 小山 玲子, 古田 美智子, 江國 大輔, 西川 悟郎, 岡崎 惠子, 志茂 加代子, 羽川 操, 森田 学

    岡山歯学会雑誌   28 ( 1 )   90 - 90   2009年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • Vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress. 査読 国際誌

    Daisuke Ekuni, Takaaki Tomofuji, Toshihiro Sanbe, Koichiro Irie, Tetsuji Azuma, Takayuki Maruyama, Naofumi Tamaki, Jun Murakami, Susumu Kokeguchi, Tatsuo Yamamoto

    Archives of Oral Biology   Vol.54 ( No.5 )   495 - 502   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    These results suggest that vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress.

    DOI: 10.1016/j.archoralbio.2009.02.006

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  • Validity of a questionnaire for periodontitis screening of Japanese employees. 査読 国際誌

    Tatsuo Yamamoto, Reiko Koyama, Naofumi Tamaki, Takayuki Maruyama, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Tetsuji Azuma, Manabu Morita

    Journal of Occupational Health   Vol.51 ( No.2 )   137 - 143   2009年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    These results suggest that the self-reported questions are useful for screening of periodontitis in 50- to 59-yr-old Japanese workers.

    DOI: 10.1539/joh.L8108

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  • Effects of improvement in periodontal inflammation by toothbrushing on serum lipopolysaccharide concentration and liver injury in rats. 査読 国際誌

    Takaaki Tomofuji, Daisuke Ekuni, Toshihiro Sanbe, Tetsuji Azuma, Naofumi Tamaki, Takayuki Maruyama, Tatsuo Yamamoto, Tatsuo Watanabe, Mutsumi Miyauchi, Takashi Takata

    Acta Odontologica Scandinavica   Vol.67 ( No.4 )   200 - 205   2009年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Toothbrushing promoted healing of periodontal lesions, decreased serum lipopolysaccharide concentration and suppressed liver injury in a rat periodontitis model.

    DOI: 10.1080/00016350902794818

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  • Effects of vitamin C intake on gingival oxidative stress in rat periodontitis. 査読 国際誌

    Takaaki Tomofuji, Daisuke Ekuni, Toshihiro Sanbe, Koichiro Irie, Tetsuji Azuma, Takayuki Maruyama, Naofumi Tamaki, Jun Murakami, Susumu Kokeguchi, Tatsuo Yamamoto

    Free Radical Biology and Medicine   Vol.46 ( No.2 )   163 - 168   2008年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Increased levels of oxidative stress due to excessive production of reactive oxygen species are involved in the pathogenesis of periodontitis. Studies suggest a negative association between plasma vitamin C level and the severity of periodontitis. We hypothesized that increases in plasma vitamin C levels after vitamin C intake might clinically reduce gingival oxidative stress in a rat periodontitis model. A ligature was placed around rat mandibular molars for 4 weeks to induce periodontitis, and the rats were then given drinking water with or without 1 g/L vitamin C for 2 weeks after the ligature was removed. The periodontitis-induced rats showed a 149% increase in 8-hydroxydeoxyguanosine level and a 40% decrease in reduced:oxidized glutathione ratio in gingival tissue. Vitamin C intake induced a 175% increase in plasma vitamin C level, resulting in an improvement in the gingival 8-hydroxydeoxyguanosine level (decreased) and in the reduced:oxidized glutathione ratio (increased). Furthermore, in ligature-induced periodontitis lesions, gene expression encoding inflammation, including interleukin-1 alpha and interleukin-1 beta, was more than twofold down-regulated by vitamin C intake. The results suggest that systemic administration of vitamin C could be clinically beneficial in improving periodontitis-induced oxidative stress by down-regulating inflammatory gene expression.

    DOI: 10.1016/j.freeradbiomed.2008.09.040

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  • 小学生における食習慣と咬合力・咬合接触面積の関係

    古田 美智子, 山中 玲子, Akhter Rahena, 小山 玲子, 友藤 孝明, 江國 大輔, 玉木 直文, 東 哲司, 山本 龍生, 岸本 悦央

    口腔衛生学会雑誌   58 ( 5 )   557 - 557   2008年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 新入生の歯科健診とアンケート評価 (第2報)歯周病のスクリーニング検査としてのアンケートの有効性

    古田 美智子, 江國 大輔, 入江 浩一郎, 小山 玲子, 三部 俊博, 山中 玲子, アクター・ラヘナ, 山本 龍生, 森田 学

    口腔衛生学会雑誌   58 ( 4 )   343 - 343   2008年8月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 新入生の歯科健診とアンケート評価 (第1報)新入生の口腔内状態とアンケート結果の概要

    江國 大輔, 古田 美智子, 入江 浩一郎, 小山 玲子, 三部 俊博, 山中 玲子, アクター・ラヘナ, 山本 龍生, 森田 学

    口腔衛生学会雑誌   58 ( 4 )   342 - 342   2008年8月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • Oxidative damage of rat liver induced by ligature-induced periodontitis and chronic ethanol consumption. 査読 国際誌

    Takaaki Tomofuji, Toshihiro Sanbe, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Takayuki Maruyama, Naofumi Tamaki, Tatsuo Yamamoto

    Archives of Oral Biology   Vol.53 ( No.12 )   1113 - 1118   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In the rat model, ligature-induced periodontitis increased plasma lipid peroxide, decreased the hepatic GSH/GSSG ratio and augmented ethanol-induced lipid peroxidation in the liver.

    DOI: 10.1016/j.archoralbio.2008.05.015

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  • 非外科的歯周治療後のメインテナンス期におけるホープレス歯の予後調査

    江國 大輔, 山本 龍生, 竹内 倫子

    日本歯周病学会会誌   50 ( 春季特別 )   183 - 183   2008年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 非外科的歯周治療後のメインテナンス期におけるホープレス歯の予後調査

    江國 大輔, 山本 龍生, 竹内 倫子

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2008   95 - 95   2008年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2008s.0.95.0

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  • 歯周組織の状態と血漿の酸化ストレス度・抗酸化力との関係

    丸山 貴之, 玉木 直文, 友藤 孝明, 江國 大輔, 山中 玲子, 竹内 倫子, 山本 龍生

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2008   52 - 52   2008年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2008s.0.52.0

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  • 歯周炎ラットへのブラッシングと血清8-OHdG濃度

    玉木 直文, 友藤 孝明, 江國 大輔, 東 哲司, 山中 玲子, 三部 俊博, 山本 龍生, 渡邊 達夫

    口腔衛生学会雑誌   57 ( 5 )   692 - 692   2007年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • ラット歯周炎モデルにおける歯肉の遺伝子発現

    江國 大輔, 三部 俊博, 友藤 孝明, 玉木 直文, 山中 玲子, 東 哲司, 山本 龍生, 渡邊 達夫

    日本歯周病学会会誌   49 ( 秋季特別 )   161 - 161   2007年8月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周炎に起因する肝病変に対するブラッシングの効果

    三部 俊博, 友藤 孝明, 江國 大輔, 山中 玲子, 東 哲治, 玉木 直文, 山本 龍生, 渡邊 達夫

    口腔衛生学会雑誌   57 ( 4 )   532 - 532   2007年8月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • ラット歯周炎モデルにおける歯肉の遺伝子発現

    江國 大輔, 三部 俊博, 友藤 孝明, 玉木 直文, 山中 玲子, 東 哲司, 山本 龍生, 渡邊 達夫

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2007   130 - 130   2007年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2007f.0.130.0

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  • ラット歯周炎モデルにおける肝病変に及ぼすブラッシングの影響

    三部 俊博, 高田 隆, 友藤 孝明, 江國 大輔, 山中 玲子, 東 哲司, 玉木 直文, 山本 龍生, 渡邊 達夫, 宮内 睦美

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2007   106 - 106   2007年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2007s.0.106.0

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  • 高コレステロール食による歯槽骨吸収にはRANKL-OPGの調節が関与する

    江國 大輔, 友藤 孝明, 山中 玲子, 草野 弘揮, 東 哲司, 三部 俊博, 山本 龍生, 渡邊 達夫

    口腔衛生学会雑誌   56 ( 5 )   724 - 724   2006年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • ラットの実験的歯周炎における機械的刺激の効果

    山本 龍生, 友藤 孝明, 江國 大輔, 山中 玲子, 東 哲司, 草野 弘揮, 三部 俊博, 渡邊 達夫

    日本歯周病学会会誌   48 ( 秋季特別 )   118 - 118   2006年9月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • ラット歯周炎モデルにおける非アルコール性脂肪性肝炎

    江國 大輔, 友藤 孝明, 山中 玲子, 草野 弘揮, 東 哲司, 三部 俊博, 玉木 直文, 山本 龍生, 渡邊 達夫, 宮内 睦美, 高田 隆

    日本歯周病学会会誌   48 ( 秋季特別 )   191 - 191   2006年9月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • ラット歯周炎モデルにおける非アルコール性脂肪性肝炎

    江國 大輔, 宮内 睦美, 高田 隆, 友藤 孝明, 山中 玲子, 草野 弘揮, 東 哲司, 三部 俊博, 玉木 直文, 山本 龍生, 渡邊 達夫

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2006   115 - 115   2006年

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    出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2006f.0.115.0

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  • ラットの実験的歯周炎における機械的刺激の効果

    山本 龍生, 友藤 孝明, 江國 大輔, 山中 玲子, 東 哲司, 草野 弘揮, 三部 俊博, 渡邊 達夫

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2006   44 - 44   2006年

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    出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2006f.0.44.0

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  • Effects of a high-cholesterol diet on cell behavior in rat periodontitis

    T. Tomofuji, H. Kusano, T. Azuma, D. Ekuni, T. Yamamoto, T. Watanabe

    Practitioner   249 ( 1675 )   752 - 756   2005年10月

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    掲載種別:研究論文(学術雑誌)  

    Studies have shown an association between periodontitis and serum cholesterol levels. We hypothesized that high dietary cholesterol could influence periodontitis as a result of proliferation of the junctional epithelium. Rats were divided into 4 groups. Two groups were fed a regular diet, and 2 groups were fed a high-cholesterol diet. One of each dietary group was treated with periodontitis-inducing agents (lipopolysaccharide and proteases), while the other was treated with pyrogen-free water. Feeding rats with a high-cholesterol diet induced an increase in blood total cholesterol and a decrease in high-density lipoprotein cholesterol. Proliferation of the junctional epithelium with increasing bone resorption was promoted by the consumption of a high-cholesterol diet. High dietary cholesterol further increased the cell-proliferative activity of the junctional epithelium induced by lipopolysaccharide and proteases. These results suggest that high dietary cholesterol can initiate and augment periodontitis in the rat periodontitis model.

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  • 高コレステロール食によるラット歯槽骨吸収におけるRANKLの発現

    江國 大輔, 友藤 孝明, 山中 玲子, 草野 弘揮, 東 哲司, 三部 俊博, 山本 龍生, 渡邊 達夫

    口腔衛生学会雑誌   55 ( 4 )   504 - 504   2005年8月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • ラット歯周病モデルによるポケット形成

    江國 大輔, 山中 玲子, 山本 龍生, 渡邊 達夫

    歯界展望   特別号 ( 健康な心と身体は口腔から-発ヨコハマ2004- )   270 - 270   2005年6月

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    記述言語:日本語   出版者・発行元:医歯薬出版(株)  

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  • ラット歯周炎モデルに対するグリチルリチン酸ジカリウム,クレイツイオンhigh power,クレイツイオンNo.1を含有する歯磨剤の効果

    山中 玲子, 山本 龍生, 江國 大輔, 坂本 友紀, 渡邊 達夫

    日本歯周病学会会誌   47 ( 春季特別 )   74 - 74   2005年3月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • ラット歯周病モデルによるポケット形成

    江國 大輔, 山中 玲子, 山本 龍生, 渡邊 達夫

    日本歯科医師会雑誌   57 ( 4 )   399 - 399   2004年7月

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    記述言語:日本語   出版者・発行元:(公社)日本歯科医師会  

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  • ブラッシングによる機械的刺激がラット歯肉細胞の細胞周期へ与える影響

    山中 玲子, 江國 大輔, 草野 弘揮, 山本 龍生, 渡邊 達夫

    岡山歯学会雑誌   23 ( 1 )   186 - 187   2004年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • LPS,プロテアーゼによる上皮基底細胞と歯根膜線維芽細胞への影響

    江國 大輔, 山中 玲子, 橘 健太郎, 山本 龍生, 渡邊 達夫

    口腔衛生学会雑誌   53 ( 5 )   620 - 620   2003年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • Beating刺激による細胞増殖能の促進

    山中 玲子, 江國 大輔, 友藤 孝明, 山本 龍生, 渡邊 達夫

    口腔衛生学会雑誌   53 ( 5 )   616 - 616   2003年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • ラット歯肉接合上皮の下方増殖におけるリポポリサッカライド濃度の影響

    山中 玲子, 江國 大輔, 橘 健太郎, 山本 龍生, 渡邊 達夫, 岸 幹二

    岡山歯学会雑誌   22 ( 1 )   241 - 242   2003年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • リポポリサッカライドとプロテアーゼの混合投与による付着歯肉の亀裂

    江國 大輔, 山中 玲子, 橘 健太郎, 山本 龍生, 渡邊 達夫

    口腔衛生学会雑誌   52 ( 5 )   723 - 723   2002年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • リポポリサッカライドとプロテアーゼの混合投与による歯周病モデル

    江國 大輔, 山中 玲子, 橘 健太郎, 山本 龍生, 渡邊 達夫

    日本歯周病学会会誌   44 ( 春季特別 )   95 - 95   2002年3月

  • Lipopolysaccharide,protease連続投与により誘発されたラット歯肉付着上皮の根尖側移動

    江國 大輔, 山中 玲子, 橘 健太郎, 山本 龍生, 渡邊 達夫

    日本歯周病学会会誌   43 ( 秋季特別 )   145 - 145   2001年9月

  • 実験的に惹起されたラット歯肉付着上皮根尖側移動におけるPCNA陽性細胞の増加

    山本 龍生, 江國 大輔, 山中 玲子, 橘 健太郎, 渡邊 達夫

    日本歯周病学会会誌   43 ( 秋季特別 )   168 - 168   2001年9月

▼全件表示

書籍等出版物

  • 新編衛生学・公衆衛生学

    安井, 利一ほか( 担当: 分担執筆)

    医歯薬出版  2023年1月  ( ISBN:9784263458556

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    総ページ数:ix, 260p   記述言語:日本語

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  • 新編衛生学・公衆衛生学

    安井, 利一( 担当: 共著)

    医歯薬出版  2021年2月  ( ISBN:9784263458556

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    総ページ数:ix, 260p   記述言語:日本語

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  • Studies on Periodontal Disease

    Springer  2014年 

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  • 総合臨床

    永井書店  2007年 

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MISC

  • 厚生労働省「歯科疾患実態調査」の二次データ分析に見る壮年期歯科疾患対策の重要性

    三宅 雅, 江國 大輔, 森田 学

    日本老年医学会雑誌   60 ( Suppl. )   154 - 155   2023年5月

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    記述言語:日本語   出版者・発行元:(一社)日本老年医学会  

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  • 揮発性硫黄化合物に対する舌背への光線力学療法の効果 無作為化比較試験

    江國 大輔, 横井 彩, 森田 学

    日本口臭学会会誌   14 ( 1 )   27 - 27   2023年5月

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    記述言語:日本語   出版者・発行元:日本口臭学会  

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  • 厚生労働省「歯科疾患実態調査」の二次データ分析に見る壮年期歯科疾患対策の重要性

    三宅 雅, 江國 大輔, 森田 学

    日本老年医学会雑誌   60 ( Suppl. )   154 - 155   2023年5月

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    記述言語:日本語   出版者・発行元:(一社)日本老年医学会  

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  • 大学生における歯周病発症と受動喫煙を含む危険因子との関係 コホート研究

    内田 悠理香, 江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, 福原 瑶子, 外山 直樹, 澤田 ななみ, 中島 千穂, 中原 桃子, 角田 伊知郎, 森田 学

    口腔衛生学会雑誌   73 ( 増刊 )   149 - 149   2023年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 令和の健口戦略;基礎研究からの発信!! 次世代へのmission Transformation,Integration and Prevention

    江國 大輔

    口腔衛生学会雑誌   73 ( 増刊 )   92 - 92   2023年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 睡眠時ブラキシズムと関連する栄養素の探索的研究

    外山 直樹, 江國 大輔, 福原 大樹, 澤田 ななみ, 森田 学

    口腔衛生学会雑誌   73 ( 増刊 )   148 - 148   2023年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 睡眠時ブラキシズムとトリプトファンとの関連 横断研究

    外山 直樹, 江國 大輔, 森田 学

    Journal of Epidemiology   33 ( Suppl.1 )   99 - 99   2023年2月

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    記述言語:日本語   出版者・発行元:(一社)日本疫学会  

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  • 大学生における主観的口腔健康評価の傾向と,口腔健康状態および口腔保健行動との関連性

    中原 桃子, 外山 直樹, 江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, 澤田 ななみ, 中島 千穂, 森田 学

    岡山歯学会雑誌   41 ( 2 )   61 - 61   2022年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 日本の大学生における歯周病の有病者率とその関連因子 10年間のデータからみる傾向

    江國 大輔, 外山 直樹, 竹内 倫子, 森田 学

    日本公衆衛生学会総会抄録集   81回   389 - 389   2022年9月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • 地域在住高齢者における認知機能低下と口腔機能およびソーシャル・キャピタルとの関連

    竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

    老年歯科医学   37 ( 2 )   109 - 109   2022年9月

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    記述言語:日本語   出版者・発行元:(一社)日本老年歯科医学会  

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  • 地域在住高齢者における認知機能低下と口腔機能およびソーシャル・キャピタルとの関連

    竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

    老年歯科医学   37 ( 2 )   109 - 109   2022年9月

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    記述言語:日本語   出版者・発行元:(一社)日本老年歯科医学会  

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  • 日本の大学生における歯周病の有病者率とその関連因子 10年間のデータからみる傾向

    江國 大輔, 外山 直樹, 竹内 倫子, 森田 学

    日本公衆衛生学会総会抄録集   81回   389 - 389   2022年9月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • 地域在住高齢者における認知機能低下と口腔機能およびソーシャル・キャピタルとの関連

    竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

    日本老年歯科医学会総会・学術大会プログラム・抄録集   33回   O4 - 05   2022年6月

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    記述言語:日本語   出版者・発行元:(一社)日本老年歯科医学会  

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  • 地域在住高齢者における認知機能低下と口腔機能およびソーシャル・キャピタルとの関連

    竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

    日本老年歯科医学会総会・学術大会プログラム・抄録集   33回   O4 - 05   2022年6月

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    記述言語:日本語   出版者・発行元:(一社)日本老年歯科医学会  

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  • 厚生労働省「歯科疾患実態調査」のヒートマップ的疾患動向分析にみる日本の医療制度改革の方向性

    三宅 雅, 江國 大輔, 森田 学

    日本老年医学会雑誌   59 ( Suppl. )   137 - 137   2022年5月

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    記述言語:日本語   出版者・発行元:(一社)日本老年医学会  

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  • 厚生労働省「歯科疾患実態調査」のヒートマップ的疾患動向分析にみる日本の医療制度改革の方向性

    三宅 雅, 江國 大輔, 森田 学

    日本老年医学会雑誌   59 ( Suppl. )   137 - 137   2022年5月

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    記述言語:日本語   出版者・発行元:(一社)日本老年医学会  

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  • 舌背への光線力学療法による口臭の改善効果の検討 ランダム化比較試験

    長崎 潤一郎, 丸山 貴之, 横井 彩, 江國 大輔, 澤田 ななみ, 森田 学

    口腔衛生学会雑誌   72 ( 増刊 )   81 - 81   2022年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • コロナ禍前とコロナ禍における大学生の睡眠時ブラキシズムの検討(横断研究)

    江國 大輔, 丸山 貴之, 横井 彩, 福原 大樹, 外山 直樹, 澤田 ななみ, 中原 桃子, 角田 伊知郎, 森田 学

    口腔衛生学会雑誌   72 ( 増刊 )   79 - 79   2022年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 睡眠時ブラキシズムの危険因子に関するコホート研究

    神野 弘貴, 外山 直樹, 江國 大輔, 森田 学

    口腔衛生学会雑誌   72 ( 増刊 )   79 - 79   2022年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 周術期のガム咀嚼トレーニングは胸部食道がん患者の咀嚼能力を維持・向上させる

    山中 玲子, 白川 靖博, 横井 彩, 中村 吉秀, 難波 奏人, 田辺 俊介, 野間 和広, 清水 一好, 内田 悠理香, 吉冨 愛子, 丸山 貴之, 江國 大輔, 森田 学

    口腔衛生学会雑誌   72 ( 増刊 )   101 - 101   2022年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 術後2週間における胃がんおよび食道がん患者の舌圧減少率と舌圧関連因子の比較

    横井 彩, 秦 浩信, 藤嶋 亮太, 篠原 敏樹, 江國 大輔, 森田 学

    日本摂食・嚥下リハビリテーション学会雑誌   26 ( 1 )   3 - 9   2022年4月

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    記述言語:日本語   出版者・発行元:(一社)日本摂食嚥下リハビリテーション学会  

    【目的】舌圧は嚥下機能の指標で、食道がん術後に低下する。その減少量は絶食期間やIntensive care unit(ICU)滞在期間に関連する。しかし、食道がんよりも嚥下機能に関わる組織への侵襲がなく、絶食期間やICU滞在日数が短い疾患の周術期における舌圧減少は不明である。胃がんの周術期における嚥下スクリーニングが注目されている。本研究の目的は、胃がん患者と食道がん患者における手術前後の舌圧減少率を比較することである。【対象と方法】対象者は、2016年1月から2017年12月までに、岡山大学病院と北海道がんセンターで手術を行い、嚥下機能評価を行った患者のうち、年齢、性別、術前舌圧、がんの進行度(ステージ)、術前Repetitive Saliva Swallowing Test(RSST)のスコアをマッチングさせた胃がん患者20名、食道がん患者20名(年齢40-88歳、男性26名、女性14名)とした。嚥下機能評価として、術前、術後1週目、2週目に舌圧とRSSTを測定した。また、舌圧に関連する因子として、年齢、性別、ステージ、術式、手術時間・出血量、挿管日数、絶食期間、発熱日数、ICU滞在期間、術後入院期間、飲酒喫煙習慣を調査した。胃がん患者と食道がん患者の周術期における舌圧の推移と術前舌圧を基準とした舌圧減少率をWilcoxonの符号付順位検定、Two-way ANOVAにて統計分析した。さらに、2群間で舌圧減少に関連する因子を比較した(Mann-Whitney U検定、χ2検定)。【結果】胃がん患者の舌圧減少率は、食道がん患者と比較し、統計学的に有意に小さかった(p=.026)。胃がん患者は、食道がん患者と比較し、周術期舌圧に影響を与える手術時間(p<.001)、絶食期間(p<.001)、ICU滞在期間(p<.001)が有意に短かった。【考察】胃がん患者の舌圧減少率が小さい理由として、胃がんの手術は、術野が口腔、咽頭、喉頭から離れており、また手術時間・絶食期間・ICU滞在期間が食道がんの手術に比べると短いことが関係している可能性がある。【結論】周術期において、胃がん患者の舌圧減少率は、食道がん患者と比較して小さかった。(著者抄録)

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    その他リンク: https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2022&ichushi_jid=J03152&link_issn=&doc_id=20220610060001&doc_link_id=%2Fcb5dvsph%2F2022%2F002601%2F001%2F0003-0009%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcb5dvsph%2F2022%2F002601%2F001%2F0003-0009%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  • 修復物の変化を評価に加えたう蝕発症と曝露因子との関係 3年間のコホート研究

    江國 大輔, 外山 直樹, 森田 学

    Journal of Epidemiology   32 ( Suppl.1 )   168 - 168   2022年1月

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    記述言語:日本語   出版者・発行元:(一社)日本疫学会  

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  • 岡山大学新入生の口腔健康状態および主観的口腔健康評価の9年間の変化

    中原 桃子, 外山 直樹, 江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, イスラム・モハマド・モニルル, 澤田 ななみ, 中島 千穂, 出口 純也, 森田 学

    口腔衛生学会雑誌   72 ( 1 )   54 - 54   2022年1月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 家庭での毎日の受動喫煙曝露は大学生の歯周病と関係する(横断研究)

    江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, 外山 直樹, イスラム・モハマド・モニルル, 澤田 ななみ, 中原 桃子, 中島 千穂, 出口 純也, 福原 瑶子, 森田 学

    口腔衛生学会雑誌   72 ( 1 )   54 - 54   2022年1月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 自立高齢者における口腔機能、栄養状態および身体的フレイルの関係

    澤田 ななみ, 竹内 倫子, 江國 大輔, 森田 学

    岡山歯学会雑誌   40 ( 2 )   33 - 34   2021年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 自立高齢者における口腔機能、栄養状態および身体的フレイルの関係

    澤田 ななみ, 竹内 倫子, 江國 大輔, 森田 学

    岡山歯学会雑誌   40 ( 2 )   33 - 34   2021年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 自立高齢者における口腔機能、栄養状態および身体的フレイルの関係 縦断調査

    竹内 倫子, 江國 大輔, 森田 学

    日本公衆衛生学会総会抄録集   80回   241 - 241   2021年11月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • 自立高齢者における口腔機能、栄養状態および身体的フレイルの関係 縦断調査

    竹内 倫子, 江國 大輔, 森田 学

    日本公衆衛生学会総会抄録集   80回   241 - 241   2021年11月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • 唾液中乳酸脱水素酵素(LDH)測定用簡易キットによるスコアの悪化にはセルフエフィカシーが関係する

    江國 大輔, 片岡 広太, 福原 大樹, 内田 瑶子, 外山 直樹, 小林 暉政, イスラム・モハマド・モニルル, 澤田 ななみ, 森田 学

    日本歯科医師会雑誌   74 ( 4 )   407 - 407   2021年7月

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    記述言語:日本語   出版者・発行元:(公社)日本歯科医師会  

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  • 口腔内局所臭気回収方法の最適化に関する研究

    松本 卓也, 高橋 有弥瑠, 松尾 明彦, 江國 大輔, 森田 学

    日本口臭学会会誌   12 ( 1 )   46 - 46   2021年5月

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    記述言語:日本語   出版者・発行元:日本口臭学会  

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  • 「口臭による日常生活への支障」に関連する要因

    佐保 輝, 横井 彩, 丸山 貴之, 竹内 倫子, 江國 大輔, 森田 学

    日本口臭学会会誌   12 ( 1 )   49 - 50   2021年5月

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    記述言語:日本語   出版者・発行元:日本口臭学会  

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  • 頭頸部がん治療による口腔機能・QOLへの影響 縦断研究

    横井 彩, 丸山 貴之, 中原 桃子, 山中 玲子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   71 ( 増刊 )   116 - 116   2021年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • ヘルスリテラシーとリスク選好が定期的な歯科健診への受診意志に与える影響

    角田 伊知郎, 森田 学, 江國 大輔, 丸山 貴之, 片岡 広太, 横井 彩, 福原 大樹, 福原 瑶子, 外山 直樹, 中原 桃子

    口腔衛生学会雑誌   71 ( 増刊 )   88 - 88   2021年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • Association between Sleep Quality and Duration and Periodontal Disease among University Students: A Cross-Sectional Study. 国際誌

    Md Monirul Islam, Daisuke Ekuni, Naoki Toyama, Ayano Taniguchi-Tabata, Kota Kataoka, Yoko Uchida-Fukuhara, Daiki Fukuhara, Hikari Saho, Nanami Sawada, Yukiho Nakashima, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   17 ( 9 )   2020年4月

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    記述言語:英語  

    The purpose of this cross-sectional study was to investigate the association between sleep quality and duration, and periodontal disease among a group of young Japanese university students. First-year students (n = 1934) at Okayama University who voluntarily underwent oral health examinations were included in the analysis. Sleep quality and duration were assessed by the Japanese version of the Pittsburgh Sleep Quality Index. Dentists examined Oral Hygiene Index-Simplified (OHI-S), probing pocket depth (PPD), and percentage of sites with bleeding on probing (BOP). Periodontal disease was defined as presence of PPD ≥ 4 mm and BOP ≥ 30%. Overall, 283 (14.6%) students had periodontal disease. Poor sleep quality was observed among 372 (19.2%) students. Mean (± standard deviation) sleep duration was 7.1 ± 1.1 (hours/night). In the logistic regression analysis, periodontal disease was significantly associated with OHI-S (odds ratio [OR]: 2.30, 95% confident interval [CI]: 1.83-2.90; p < 0.001), but not sleep quality (OR: 1.09, 95% CI: 0.79-1.53; p = 0.577) or sleep duration (OR: 0.98, CI: 0.87-1.10; p = 0.717). In conclusion, sleep quality and duration were not associated with periodontal disease among this group of young Japanese university students.

    DOI: 10.3390/ijerph17093034

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  • 腸内細菌の有無が胎児内軟骨成長に与える影響

    内田瑶子, 服部高子, 福原大樹, FU Shanqi, 近藤星, 桑原美穂, ISLAM Monirul, 片岡広太, 江國大輔, 久保田聡, 森田学

    日本軟骨代謝学会プログラム・抄録集   33rd   2020年

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  • 日中の咬筋の筋活動は歯周炎の進行と関係する:コホート研究

    江國大輔, 加藤聖也, 川上滋央, 丸山貴之, 片岡広太, 内田瑤子, 福原大樹, 外山直樹, 皆木省吾, 森田学

    日本疫学会学術総会講演集(Web)   30th   2020年

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  • 歯周病ラットにおける血清miRNAの肝臓mRNAへの影響

    杉浦 嘉雄, 米田 俊樹, 藤森 浩平, 丸山 貴之, 宮井 久敬, 小林 暉政, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   69 ( 増刊 )   182 - 182   2019年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • ラット歯周病モデルにおける血清miRNAと肝臓への影響

    杉浦 嘉雄, 米田 俊樹, 藤森 浩平, 丸山 貴之, 宮井 久敬, 小林 暉政, 江國 大輔, 友藤 孝明, 森田 学

    岡山歯学会雑誌   37 ( 2 )   81 - 81   2018年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 化学放射線療法中の口腔粘膜炎管理を行った症例

    益成 美保, 水野 裕文, 丸山 貴之, 横井 彩, 小林 暉政, 佐々木 禎子, 志茂 加代子, 三浦 留美, 水川 展吉, 江國 大輔, 森田 学

    口腔衛生学会雑誌   68 ( 4 )   231 - 237   2018年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

    頭頸部がんに対する化学放射線療法(CRT)の有害事象である口腔粘膜炎による疼痛は、患者のQOLを低下させ、がん治療の完遂を困難にする。今回、われわれは術後CRTを受けている患者に対して口腔管理を行い、口腔粘膜炎および疼痛を制御した結果、がん治療の完遂に貢献できた症例を報告する。患者は67歳、男性である。右側舌がん(T2N2bM0)に対して舌可動部半側切除術、右側頸部郭清術、遊離前外側大腿皮弁による再建術を施行した。術後の病理組織検査より切除断端陽性、頸部リンパ節の節外浸潤を認めたため、術後CRT(抗がん剤:シスプラチン、5-フルオロウラシル;2クール、強度変調放射線治療60Gy)を行うこととなった。CRT前から、口腔管理および衛生実地指導を継続して行った。CRT開始時はプラークの除去を中心とした口腔管理を行った。22Gy照射時点で口腔粘膜炎(Grade2)が発症したものの、その後軽度疼痛を認める程度で経過していた。42Gy照射時点で口腔粘膜炎の悪化(Grade3)および自発痛・接触痛を認めたため、治療完遂まで毎日、歯科衛生士がプラークの除去に加え、口腔粘膜保護を目的とした口腔管理を行った。その結果、56Gy照射時点で口腔粘膜炎は改善傾向を示し(Grade2)、疼痛も緩和され、CRT完遂に至った。術後CRT開始直後から口腔粘膜炎の状態に応じた口腔管理を行うことで、疼痛の制御が成功し、CRTの完遂に貢献できたと考えられる。(著者抄録)

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    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2018&ichushi_jid=J00440&link_issn=&doc_id=20181113220005&doc_link_id=%2Fdk1dehea%2F2018%2F006804%2F005%2F0231-0237%26dl%3D0&url=http%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdk1dehea%2F2018%2F006804%2F005%2F0231-0237%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  • Relationship between severity of periodontitis and masseter muscle activity during waking and sleeping hours

    Seiya Kato, Daisuke Ekuni, Shigehisa Kawakami, Acing Habibie Mude, Manabu Morita, Shogo Minagi

    Archives of Oral Biology   90   13 - 18   2018年6月

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    記述言語:英語   出版者・発行元:Elsevier Ltd  

    Objective: This study was conducted to investigate whether the masseter muscle activity shows any specific pattern in relation to the severity of periodontitis. Design: Sixteen subjects with no or mild periodontitis (NMP group) and 15 subjects with moderate or severe periodontitis (MSP group) were enrolled. Plasma IgG antibody titer was examined using Porphyromonas gingivalis as a bacterial antigen. Surface electromyography (EMG) of the masseter muscles was continuously recorded using an ambulatory surface EMG recording device while patients were awake and asleep. Masseter muscle activity was analyzed using intensities of 5%–10% maximal voluntary clenching (MVC), 10%–20% MVC, and &gt
    20% MVC. Furthermore, EMG levels of 20% MVC were adopted as the threshold for analysis of phasic, tonic, and mixed EMG activities. The cumulative duration of masseter muscle activity and bruxism episodes was calculated as duration per hour. Results: There was no significant difference in plasma IgG antibody titers against P. gingivalis between the NMP and MSP groups (p = 0.423). During waking hours, the duration of masseter muscle activity with an intensity of &gt
    20% MVC was significantly longer in the MSP group than in the NMP group (p = 0.037). During sleeping hours, the duration of masseter muscle activity at all MVC intensities was significantly longer in the MSP group than in the NMP group (all p &lt
    0.05). Additionally, the duration of phasic and mixed episodes was significantly longer in the MSP group than those in the NMP group while both awake and asleep (all p &lt
    0.05). Conclusions: The results of this study suggested that masseter muscle activity might be related to the severity of periodontitis.

    DOI: 10.1016/j.archoralbio.2018.02.021

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  • 口腔内ジメチルサルファイド濃度と全身疾患および服薬との関係

    横井 彩, 竹内 倫子, 宮井 久敬, 江國 大輔, 森田 学

    日本口臭学会会誌   9 ( 1 )   38 - 39   2018年5月

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    記述言語:日本語   出版者・発行元:日本口臭学会  

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  • 歯周組織における加齢に伴う酸化ストレスの増加に対するコーヒー摂取の影響

    小林 暉政, 東 哲司, 杉浦 嘉雄, 宮井 久敬, 米田 俊樹, 丸山 貴之, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   68 ( 増刊 )   182 - 182   2018年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 大学生における口腔関連QOLの変化に影響を及ぼす因子の検討 縦断研究

    佐保 輝, 竹内 真由, 稲生 智秋, Monirul Islam Md, 杉浦 嘉雄, 外山 直樹, 内田 瑶子, 横井 彩, 谷口 綾乃, 片岡 広太, 米田 俊樹, 東 哲司, 江國 大輔, 森田 学

    口腔衛生学会雑誌   68 ( 増刊 )   109 - 109   2018年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 歯科衛生士教育における模擬患者の違いによる実習効果の比較

    木村 優子, 江國 大輔, 森田 学, 太田 正美

    口腔衛生学会雑誌   68 ( 増刊 )   129 - 129   2018年4月

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  • Unusual oral mucosal microbiota after hematopoietic cell transplantation with glycopeptide antibiotics: potential association with pathophysiology of oral mucositis 国際誌

    Misato Muro, Yoshihiko Soga, Tomoko Higuchi, Kota Kataoka, Daisuke Ekuni, Yoshinobu Maeda, Manabu Morita

    Folia Microbiologica   63 ( 5 )   1 - 11   2018年3月

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    記述言語:英語   出版者・発行元:Springer Netherlands  

    Severe oral mucositis occurs frequently in patients receiving hematopoietic stem cell transplantation (HCT). Oral mucosal bacteria can be associated with progression of oral mucositis, and systemic infection may occur via ulcerative oral mucositis. However, little information is available regarding the oral microbiota after HCT. Here, PCR-denaturing gradient gel electrophoresis (DGGE) was performed to characterize the oral mucosal microbiota, which can be affected by antibiotics, before and after HCT. Sixty reduced-intensity HCT patients were enrolled. Three patients with the least antibiotic use (quinolone prophylaxis and/or β-lactam monotherapy group) and three patients with the most antibiotic use (β-lactam-glycopeptide combination therapy group) were selected. Bacterial DNA samples obtained from the oral mucosa before and after HCT were subjected to PCR-DGGE. The trajectory of oral mucositis was evaluated. The oral mucosal microbiota in the β-lactam-glycopeptide combination therapy group was different from that in the quinolone prophylaxis and/or β-lactam monotherapy group, and Staphylococcus spp. and Enterococcus spp. were identified. Lautropia mirabilis was dominant in one patient. Ulcerative oral mucositis was observed only in the β-lactam-glycopeptide combination therapy group. In conclusion, especially with the use of strong antibiotics, such as glycopeptides, the oral mucosal microbiota differed completely from that under normal conditions and consisted of Staphylococcus spp., Enterococcus spp., and unexpectedly L. mirabilis. The normal oral microbiota consists not only of bacteria, but these unexpected bacteria could be involved in the pathophysiology as well as systemic infection via oral mucositis. Our results can be used as the basis for future studies in larger patient populations.

    DOI: 10.1007/s12223-018-0596-1

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  • Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium

    Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Daiki Fukuhara, Yoko Uchida, Kota Kataoka, Shuichiro Kobayashi, Takeshi Kikuchi, Akio Mitani, Yoshihiro Shimazaki, Manabu Morita

    Archives of Oral Biology   87   72 - 78   2018年3月

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    記述言語:英語   出版者・発行元:Elsevier Ltd  

    Objective Increasing age is a potential risk factor for periodontal tissue breakdown, which may be affected by commensal flora. The aim of this study evaluated age-related changes in CD4+ T cells, C-C chemokine ligand 5 (CCL5), interleukin (IL)-17A, and receptor activator of nuclear factor-kappa B ligand (RANKL) expression using germ-free (GF) and conventionally reared (SPF) mice. Design GF and SPF mice at 8 (n = 6/group) and 22 weeks old (n = 6/group) were used. Immunohistochemical analyses were performed to determine the effects of aging on protein expression in periodontal tissues. Age-related changes in alveolar bone were quantified using micro-CT analysis. Results SPF mice, but not GF mice, showed an age-related increase in alveolar bone loss (P &lt
    0.01). SPF mice at 22 weeks of age increased expression of CD4+ T cells, CCL5, IL-17A, and RANKL compared to those at 8 weeks of age in connective tissue and alveolar bone surface (P &lt
    0.01). Furthermore, there was increased CD4+ T cells, which were co-expressed with IL-17A and RANKL in SPF mice at 22 weeks of age. On the other hand, the GF mice did not show any significant differences in CD4+ T cells, CCL5, IL-17A and RANKL expression between the two age groups. Conclusions SPF mice induced an age-related increase in CD4+ T cells co- expressed with IL-17A and RANKL, with occurring alveolar bone loss. In contrast, GF mice did not show age-related changes in CD4+ T cell migration and cytokine expression.

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  • 職域における早食い、口腔内状態およびメタボリックシンドローム発症との関連

    横井 彩, 江國 大輔, 米田 俊樹, 森田 学

    口腔衛生学会雑誌   68 ( 1 )   9 - 14   2018年1月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

    メタボリックシンドローム(Metabolic Syndrome:MetS)のリスク因子として早食いが挙げられる。また近年、MetsSは歯の喪失やう蝕など口腔内状態との関連も注目されている。しかし、MetS、早食いおよび口腔内状態を総合的に検討した報告は少ない。そこで、本縦断研究は、職域における早食いおよび口腔内状態とMetS発症との関連について検討することを目的とした。対象者は、某事業所における平成24・27年度定期健診をどちらも受診した従業員のうち、平成24年度MetSに該当しなかった男性114名(36〜63歳)を分析対象者とした。診査項目は、身長、体重、腹囲、血圧、空腹時血糖値、中性脂肪、HDLコレステロール値、口腔内状態、生活習慣(早食いの有無、食・運動・飲酒・喫煙習慣)とした。平成27年度健診結果から、MetS発症群と非発症群に分け、早食いの習慣および口腔内状態との関連を分析した。統計分析にはχ2検定、Fisherの直接法およびMann-Whitney U検定を用いた。3年後にMetSを発症した者(16名)は、全員早食いの自覚があった。また、MetS発症において、早食いと腹囲異常による相加効果がみられた。結論として、早食いで腹囲異常のある者はMetS発症のリスクが高いことがわかった。一方で、口腔内状態との直接的な関連はみられなかった。(著者抄録)

    DOI: 10.5834/jdh.68.1_9

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    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2018&ichushi_jid=J00440&link_issn=&doc_id=20180207240002&doc_link_id=%2Fdk1dehea%2F2018%2F006801%2F002%2F0009-0014%26dl%3D0&url=http%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdk1dehea%2F2018%2F006801%2F002%2F0009-0014%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  • Type D personality and periodontal disease in university students: A prospective cohort study. 国際誌

    Mizutani S, Ekuni D, Yamane-Takeuchi M, Azuma T, Taniguchi-Tabata A, Tomofuji T, Iwasaki Y, Morita M

    J Health Psychol   23 ( 5 )   754 - 762   2018年

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  • The effects of non-surgical periodontal treatment on glycemic control, oxidative stress balance and quality of life in patients with type 2 diabetes: A randomized clinical trial 国際誌

    Hirofumi Mizuno, Daisuke Ekuni, Takayuki Maruyama, Kota Kataoka, Toshiki Yoneda, Daiki Fukuhara, Yoshio Sugiura, Takaaki Tomofuji, Jun Wada, Manabu Morita

    PLOS ONE   12 ( 11 )   25 - 36   2017年11月

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    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Aim
    The purpose of this study was to investigate the effects of non-surgical periodontal treatment on hemoglobinA1c (HbA1c) levels, oxidative stress balance and quality of life (QOL) in patients with type 2 diabetes mellitus (T2DM) compared to no periodontal treatment (simple oral hygiene instructions only).
    Methods
    The design was a 6-month, single-masked, single center, randomized clinical trial. Patients had both T2DM and chronic periodontitis. Forty participants were enrolled between April 2014 and March 2016 at the Nephrology, Diabetology and Endocrinology Department of Okayama University Hospital. The periodontal treatment group (n = 20) received non-surgical periodontal therapy, including scaling and root planing plus oral hygiene instructions, and consecutive supportive periodontal therapy at 3 and 6 months. The control group (n = 17) received only oral hygiene instructions without treatment during the experimental period. The primary study outcome was the change in HbA1c levels from baseline to 3 months. Secondary outcomes included changes in oxidative stress balance (Oxidative-INDEX), the Diabetes Therapy-Related QOL and clinical periodontal parameters from baseline to 3 months and baseline to 6 months.
    Results
    Changes in HbA1c in the periodontal treatment group were not significantly different with those in the control group at 3 and 6 months. Systemic oxidative stress balance and QOL significantly improved in the periodontal treatment group compared to the control group at 3 months. In the subgroup analysis (moderately poor control of diabetes), the decrease in HbA1c levels in the periodontal treatment group was greater than that in the control group at 3 months but not significant.
    Conclusions
    In T2DM patients, non-surgical periodontal treatment improved systemic oxidative stress balance and QOL, but did not decrease HbA1c levels at 3 months follow-up.

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  • Topical application of ointment containing 0.5% green tea catechins suppresses tongue oxidative stress in 5-fluorouracil administered rats 国際誌

    Hisataka Miyai, Takayuki Maruyama, Takaaki Tomofuji, Toshiki Yoneda, Tetsuji Azuma, Hirofumi Mizuno, Yoshio Sugiura, Terumasa Kobayashi, Daisuke Ekuni, Manabu Morita

    ARCHIVES OF ORAL BIOLOGY   82   247 - 255   2017年10月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats.
    Design: Male Wistar rats (n = 28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1% or 0.5% green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus.
    Results: After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P &lt; 0.05). On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group (P &lt; 0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5% green tea catechin group than in the positive control group (P &lt; 0.05).
    Conclusions: Topical application of ointment containing 0.5% green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.

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  • 大学生における歯ぎしりの自覚と不正咬合との関連 前向きコホート研究

    外山 直樹, 江國 大輔, 入江 浩一郎, 竹内 真由, 谷口 綾乃, 森田 学

    日本公衆衛生学会総会抄録集   76回   317 - 317   2017年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • 大学生における歯周炎と学部系統との関連

    谷口 綾乃, 江國 大輔, 竹内 真由, 森田 学

    日本公衆衛生学会総会抄録集   76回   464 - 464   2017年10月

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  • Hyperlipidemia is involved in apoptosis in rat submandibular glands 国際誌

    Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Toshiki Yoneda, Yoshihiro Shimazaki, Manabu Morita

    ARCHIVES OF ORAL BIOLOGY   81   136 - 140   2017年9月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: The aim of the present study was to investigate the effects of hyperlipidemia on histological changes and apoptosis in submandibular glands using apolipoprotein E (apoE)-deficient rats.
    Design: Histopathological findings related to induced apoptosis in the submandibular glands were compared between apoE-deficient rats (n = 6; male; age, 16 weeks) and the corresponding wild-type rats (n = 6).
    Results: ApoE-deficient rats showed significantly higher plasma levels of oxidized low-density lipoprotein (LDL), total cholesterol, very LDL and LDL, and lower plasma levels of high-density lipoprotein when compared to control rats (P &lt; 0.05). Lipid deposition in the submandibular gland was observed in apoE-deficient rat group and in none of the control group. Significant increases in vacuolization and apoptosis in acinar cells were observed in apoE-deficient rats, as compared to control rats (P &lt; 0.05). The number of active caspase-3-positive cells was also higher in the apoE-deficient rat group when compared with the control group (P &lt; 0.01).
    Conclusions: According to our results, hyperlipidemia induced apoptosis in apoE-deficient rat submandibular glands. Oxidized LDL generation in case of hyperlipidemia may trigger off a reaction of apoptotic acinar cells with vacuolization in the submandibular glands.

    DOI: 10.1016/j.archoralbio.2017.05.004

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  • 大学病院に勤務する医療技術部門職員および事務部職員における歯科用語の認知度の違い

    城本 ゆき子, 三浦 留美, 高坂 由紀奈, 江國 大輔, 佐々木 朗, 飯田 征二

    日本歯科衛生学会雑誌   12 ( 1 )   157 - 157   2017年8月

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    記述言語:日本語   出版者・発行元:日本歯科衛生学会  

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  • 勤務形態が口腔保健行動および口腔状態に与える影響

    外山 直樹, 入江 浩一郎, 谷口 綾乃, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   67 ( 3 )   206 - 206   2017年7月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 抗がん剤投与ラットへのカテキン局所塗布による抗酸化作用の検討

    小林 暉政, 宮井 久敬, 丸山 貴之, 友藤 孝明, 東 哲司, 米田 俊樹, 杉浦 嘉雄, 江國 大輔, 森田 学

    口腔衛生学会雑誌   67 ( 3 )   203 - 203   2017年7月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • Associations between dental knowledge, source of dental knowledge and oral health behavior in Japanese university students: A cross-sectional study 国際誌

    Ayano Taniguchi-Tabata, Daisuke Ekuni, Shinsuke Mizutani, Mayu Yamane-Takeuchi, Kota Kataoka, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

    PLOS ONE   12 ( 6 )   1 - 12   2017年6月

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    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    The aim of this study was to investigate the associations between dental knowledge, the source of dental knowledge and oral health behavior in a group of students at a university in Japan. A total of 2,220 university students (1,276 males, 944 females) volunteered to undergo an oral examination and answer a questionnaire. The questionnaire assessed dental knowledge, the source of dental knowledge and oral health behavior (e.g., daily frequency of tooth brushing, use of dental floss and regular dental checkups). The odds ratio and 95% confidence interval for oral health behavior based on dental knowledge and source of dental knowledge were calculated using logistic regression models. Of the participants, 1,266 (57.0%) students obtained dental knowledge from dental clinics, followed by school (39.2%) and television (29.1%). Logistic regression analyses indicated that use of dental floss was significantly associated with source of dental knowledge from dental clinics (P = 0.006). Receiving regular dental checkups was significantly associated with source of dental knowledge; the positive source was dental clinic (P &lt; 0.001) and the negative sources were school (P = 0.004) and television (P = 0.018). Dental clinic was the most common source of dental knowledge and associated with better oral health behavior among the Japanese university students in this study.

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  • Expression of Salivary miR-203a-3p Was Related with Oral Health-Related Quality of Life in Healthy Volunteers 国際誌

    Terumasa Kobayashi, Takaaki Tomofuji, Tatsuya Machida, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Akiko Hirose, Manabu Morita

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   18 ( 6 )   372 - 373   2017年6月

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    記述言語:英語   出版者・発行元:MDPI AG  

    Oral health-related quality of life (OHRQoL) is a multidimensional construct that involves subjective evaluation of an individual's oral health. Although it is difficult to evaluate OHRQoL biologically, recently, it has been reported that circulating microRNAs (miRNAs) in several body fluids could reflect various health conditions. The aim of this pilot study was to investigate whether salivary miRNAs expression differs according to OHRQoL in healthy volunteers. Forty-six volunteers (median age, 23.0 years) were recruited, and their OHRQoL was assessed using the Japanese version of the Oral Health Impact Profile (OHIP-J). Then, we compared salivary microRNA profiles of the high-OHRQoL group (&lt;= 25th percentile score of OHIP-J) and the low-OHRQoL group (&gt;= 75th percentile score of OHIP-J) using the polymerase chain reaction (PCR) array and the quantitative real-time PCR. There were no significant differences between the two groups in terms of oral health status. In the PCR array, miR-203a-3p and miR-30b-5p were significantly more expressed in the low-OHRQoL group (p &lt; 0.05). Quantitative real-time PCR assay also showed that miR-203a-3p was more highly expressed in the low-OHRQoL group than in the high-OHRQoL group (p &lt; 0.05). These observations suggest that expression of salivary miR-203a-3p was related with OHRQoL in healthy volunteers.

    DOI: 10.3390/ijms18061263

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  • Association between periodontitis and prognosis of pancreatobiliary tract cancer: A pilot study. 国際誌

    Takayuki Maruyama, Takaaki Tomofuji, Tatsuya Machida, Hironari Kato, Koichiro Tsutsumi, Daisuke Uchida, Akinobu Takaki, Toshiki Yoneda, Hisataka Miyai, Hirofumi Mizuno, Daisuke Ekuni, Hiroyuki Okada, Manabu Morita

    Molecular and clinical oncology   6 ( 5 )   683 - 687   2017年5月

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    記述言語:英語  

    Several studies have indicated that periodontitis is a risk factor for cancer. However, the association between periodontitis and the prognosis of pancreatobiliary tract cancer remains unclear. The aim of this pilot study was to investigate the association between periodontitis and prognosis of pancreatobiliary tract cancer. A total of 22 patients diagnosed with pancreatobiliary tract cancer were analyzed. Oral health status, including severity of periodontitis, general health status and biochemical serum markers were evaluated. The Kaplan-Meier method and Cox proportional hazards model were used to assess factors affecting the prognosis of pancreatobiliary tract cancer. The Kaplan-Meier analysis demonstrated that low body mass index, high concentration of serum C-reactive protein (CRP) and severe periodontitis were significant prognostic factors for survival rate. The Cox proportional hazards model revealed that serum carbohydrate antigen 19-9 concentration [hazard ratio (HR)=1.002; 95% confidence interval (CI): 1.000-1.004] and serum CRP concentration (HR=2.57; 95% CI: 1.15-5.74) were significantly associated with the prognosis of pancreatobiliary tract cancer. In addition, cancer patients with severe periodontitis had higher serum CRP concentrations compared with those without severe periodontitis. Therefore, severe periodontitis indirectly affected the prognosis of pancreatobiliary tract cancer through promoting systemic inflammation.

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  • 唾液中microRNAと歯周病の重症度との関連

    藤森 浩平, 米田 俊樹, 友藤 孝明, 江國 大輔, 東 哲司, 丸山 貴之, 町田 達哉, 水野 裕文, 森田 学

    口腔衛生学会雑誌   67 ( 増刊 )   168 - 168   2017年4月

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  • 2型糖尿病患者における非外科的歯周治療による酸化ストレスの改善 ランダム化比較試験

    杉浦 嘉雄, 水野 裕文, 福原 大樹, 丸山 貴之, 江國 大輔, 米田 俊樹, 片岡 広太, 川端 勇也, 水谷 慎介, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   67 ( 増刊 )   119 - 119   2017年4月

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  • 口腔衛生学分野のテーマをアウトカム基盤型教育で行うには 予防歯科のアウトカム基盤型教育

    江國 大輔

    口腔衛生学会雑誌   67 ( 増刊 )   77 - 78   2017年4月

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  • Preventive Effects of Drinking Hydrogen-Rich Water on Gingival Oxidative Stress and Alveolar Bone Resorption in Rats Fed a High-Fat Diet 国際誌

    Toshiki Yoneda, Takaaki Tomofuji, Muneyoshi Kunitomo, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Tatsuya Machida, Hisataka Miyai, Kouhei Fujimori, Manabu Morita

    NUTRIENTS   9 ( 1 )   830 - 833   2017年1月

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    記述言語:英語   出版者・発行元:MDPI AG  

    Obesity induces gingival oxidative stress, which is involved in the progression of alveolar bone resorption. The antioxidant effect of hydrogen-rich water may attenuate gingival oxidative stress and prevent alveolar bone resorption in cases of obesity. We examined whether hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption in obese rats fed a high-fat diet. Male Fischer 344 rats (n = 18) were divided into three groups of six rats each: a control group (fed a regular diet and drinking distilled water) and two experimental groups (fed a high-fat diet and drinking distilled water or hydrogen-rich water). The level of 8-hydroxydeoxyguanosine was determined to evaluate oxidative stress. The bone mineral density of the alveolar bone was analyzed by micro-computerized tomography. Obese rats, induced by a high-fat diet, showed a higher gingival level of 8-hydroxydeoxyguanosine and a lower level of alveolar bone density compared to the control group. Drinking hydrogen-rich water suppressed body weight gain, lowered gingival level of 8-hydroxydeoxyguanosine, and reduced alveolar bone resorption in rats on a high-fat diet. The results indicate that hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption by limiting obesity.

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  • Validity of a New Kit Measuring Salivary Lactate Dehydrogenase Level for Screening Gingivitis 国際誌

    Daisuke Ekuni, Mayu Yamane-Takeuchi, Kota Kataoka, Aya Yokoi, Ayano Taniguchi-Tabata, Hirofumi Mizuno, Hisataka Miyai, Yoko Uchida, Daiki Fukuhara, Yoshio Sugiura, Takaaki Tomofuji, Manabu Morita

    DISEASE MARKERS   2017   2434 - 2441   2017年

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    記述言語:英語   出版者・発行元:HINDAWI LTD  

    Aim. The aim of this study was to determine the usefulness of a new kit that can evaluate salivary lactate dehydrogenase (LD) level in real time for screening gingivitis. Materials and Methods. The study included 70 systemic healthy volunteers [29 males and 41 females; mean age +/- SD: 24.1 +/- 2.6 years]. Resting saliva was collected from each participant and LD level was evaluated in real time using the kit (a color-changing sheet with an integer scale ranging from 1 to 10). A dentist measured probing pocket depth, clinical attachment level, and the proportion of sites with bleeding on probing (% BOP) at six sites on all teeth. Gingivitis was diagnosed when the BOP value was &gt;= 20%. Results. Salivary LD level was positively correlated with mean % BOP (odds ratio: 1.47, 95% confidence interval: 1.132-1.916, and P &lt; 0.001) in a logistic regression model. The sensitivity and specificity of the kit were 0.89 and 0.98, respectively, at a cut-off value of 8.0 for LD level. Conclusions. The new kit for measurement of salivary LD level may be a useful tool to screen for gingivitis in young adults, which contributes to early detection of future periodontitis.

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  • 動機づけ面接法(MI)により高齢者の口腔衛生の改善ができた1症例.

    千神 八重子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   67 ( 1 )   29 - 34   2017年

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  • ラット歯周炎モデルに対する天然植物由来成分配合歯磨剤の効果.

    米田 俊樹, 東 哲司, 友藤 孝明, 江國 大輔, 片岡 広太, 山根 真由, 川端 勇也, 國友 宗義, 森田 学

    口腔衛生学会雑誌   67 ( 1 )   11 - 17   2017年

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  • Associations among oral health-related quality of life, subjective symptoms, clinical status, and self-rated oral health in Japanese university students: a cross-sectional study 国際誌

    Mayu Yamane-Takeuchi, Daisuke Ekuni, Shinsuke Mizutani, Kota Kataoka, Ayano Taniguchi-Tabata, Tetsuji Azuma, Michiko Furuta, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

    BMC ORAL HEALTH   16 ( 1 )   127 - 127   2016年11月

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    記述言語:英語   出版者・発行元:BIOMED CENTRAL LTD  

    Background: The present study aimed to elucidate the associations among self-rated oral health, clinical oral health status, oral health behaviors, subjective oral symptoms, and oral health-related quality of life (OHRQoL) in a group of Japanese university students.
    Methods: Of 2051 participants, 2027 (98.83%) students received an optional oral examination and answered a questionnaire including items regarding age, sex, self-rated oral health, oral health behaviors, subjective oral symptoms, and OHRQoL [The Oral Health Impact Profile (OHIP)-14]. On oral examination, the decayed, missing, and filled teeth (DMFT) score, Community Periodontal Index (CPI), the percentage of teeth showing bleeding on probing (%BOP), and malocclusion were recorded. Structural equation modelling (SEM) analysis was used to test associations.
    Results: The mean score (+/- SD) of OHIP-14 was 1.92 +/- 5.47. In the SEM analysis, the final model showed that self-rated oral health, oral pain, malocclusion, and the DMFT score were directly associated with the OHRQoL, and subjective symptoms of temporomandibular disorders (TMD) and recurrent aphthous stomatitis were both directly and indirectly associated (p &lt; 0.05). CPI, %BOP, and oral health behaviors were excluded from the final model.
    Conclusions: OHRQoL was associated with self-related oral health, subjective symptoms of TMD, oral pain and stomatitis, DMFT, and malocclusion in this group of Japanese university students.

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  • Visualization of Oxidative Stress Induced by Experimental Periodontitis in Keap1-Dependent Oxidative Stress Detector-Luciferase Mice 国際誌

    Kota Kataoka, Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Muneyoshi Kunitomo, Yoko Uchida, Daiki Fukuhara, Manabu Morita

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   17 ( 11 )   2016年11月

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    記述言語:英語   出版者・発行元:MDPI AG  

    The aim of this study was to investigate whether a Keap1-dependent oxidative stress detector-luciferase (OKD-LUC) mouse model would be useful for the visualization of oxidative stress induced by experimental periodontitis. A ligature was placed around the mandibular first molars for seven days to induce periodontitis. Luciferase activity was measured with an intraperitoneal injection of D-luciferin on days 0, 1, and 7. The luciferase activity in the periodontitis group was significantly greater than that in the control group at seven days. The expressions of heme oxygenase-1 (HO-1) and malondialdehyde in periodontal tissue were significantly higher in the periodontitis group than in the control group. Immunofluorescent analysis confirmed that the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) occurred more frequently in the periodontitis group than in the control group. This study found that under oxidative stress induced by experimental periodontitis, the Nrf2/antioxidant defense pathway was activated and could be visualized from the luciferase activity in the OKD-LUC model. Thus, the OKD-LUC mouse model may be useful for exploring the mechanism underlying the relationship between the Nrf2/antioxidant defense pathway and periodontitis by enabling the visualization of oxidative stress over time.

    DOI: 10.3390/ijms17111907

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  • miR-1246 and miR-4644 in salivary exosome as potential biomarkers for pancreatobiliary tract cancer 国際誌

    Tatsuya Machida, Takaaki Tomofuji, Takayuki Maruyama, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Hisataka Miyai, Hirofumi Mizuno, Hironari Kato, Koichiro Tsutsumi, Daisuke Uchida, Akinobu Takaki, Hiroyuki Okada, Manabu Morita

    ONCOLOGY REPORTS   36 ( 4 )   2375 - 2381   2016年10月

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    記述言語:英語   出版者・発行元:SPANDIDOS PUBL LTD  

    Pancreatobiliary tract cancer is a highly fatal cancer. Detection of pancreatobiliary tract cancer is difficult because it lacks typical clinical symptoms and because of its anatomical location. Biomarker discovery is therefore important to detect pancreatobiliary tract cancer in its early stage. A study demonstrated that expression levels of miR-1246, miR-3976, miR-4306, and miR-4644 in serum exosomes were higher in pancreatic cancer patients than these levels in healthy control participants. Supposing that microRNA (miRNA) expression profiles in saliva are similar to those in serum, four miRNAs (miR-1246, miR-3976, miR-4306, and miR-4644) in salivary exosomes may also be useful for detection of pancreatobiliary tract cancer. In this study, it was examined whether these miRNAs could be used as biomarkers for pancreatobiliary tract cancer. Twelve pancreatobiliary tract cancer patients and 13 healthy control participants were analyzed as a cancer and a control group, respectively. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. Using quantitative real-time PCR (RT-qPCR), the relative expression ratios of miR-1246 and miR-4644 were significantly higher in the cancer group than these ratios in the control group. Receiver operating characteristic (ROC) curves were constructed to analyze the discrimination power of these miRNAs. For miR-1246, the results yielded an area under the curve (AUC) of 0.814 (P=0.008). For miR-4644, the results yielded an AUC of 0.763 (P=0.026). For the combination of miR-1246 and miR-4644, the results yielded an increased AUC of 0.833 (P=0.005). This pilot study suggests that miR-1246 and miR-4644 in salivary exosomes could be candidate biomarkers for pancreatobiliary tract cancer.

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  • MicroRNAs as serum biomarkers for periodontitis 国際誌

    Takaaki Tomofuji, Toshiki Yoneda, Tatsuya Machida, Daisuke Ekuni, Tetsuji Azuma, Kota Kataoka, Takayuki Maruyama, Manabu Morita

    JOURNAL OF CLINICAL PERIODONTOLOGY   43 ( 5 )   418 - 425   2016年5月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Aim: Studies demonstrated that periodontitis modulates microRNA (miRNAs) expression rates in periodontal tissue. However, the relationship between periodontitis and miRNAs profile in circulation remains unclear. In this study, we investigated the effects of periodontitis on serum miRNAs profile in a rat model.
    Material and Methods: Male Wistar rats (n=32, 8weeks old) were divided into four groups of eight rats each. The control groups received no treatment for 2 or 4weeks. In the other two groups, periodontitis was ligature induced for 2 or 4weeks. Serum miRNAs expression profiles of each group were compared.
    Results: Ligation around teeth induced periodontal inflammation at 2weeks and periodontal tissue destruction at 4weeks. Microarray results showed that 25 miRNAs were expressed with a &lt;0.5 or &gt;2 difference between the control and periodontitis groups at 4weeks. Results of real-time PCR revealed that the periodontitis group up-regulated expression rates of serum miR-207 and miR-495 at 2weeks, and miR-376b-3p at 4weeks (p&lt;0.05).
    Conclusion: Serum miRNAs (miR-207, miR-495, and miR-376b-3p) could be valuable biomarkers for periodontitis.

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  • 食道癌の術後患者に対する歯科専門職による口腔のケアの効果

    水野 裕文, 水谷 慎介, 丸山 貴之, 横井 彩, 谷口 彩乃, 山中 玲子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   66 ( 2 )   219 - 219   2016年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 歯肉炎のスクリーニングにおける唾液中LDH簡易測定キットの有効性

    吉田 宗生, 加藤 壮真, 山根 真由, 江國 大輔, 横井 彩, 丸山 貴之, 山中 玲子, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   66 ( 2 )   294 - 294   2016年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 口腔内アセトアルデヒド濃度と舌背の細菌数との関係

    加藤 壮真, 吉田 宗生, 横井 彩, 丸山 貴之, 江國 大輔, 山根 真由, 山中 玲子, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   66 ( 2 )   296 - 296   2016年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 歯科衛生士教育における保護者を模擬患者とした実習の取り組みと効果

    木村 優子, 江國 大輔, 森田 学, 太田 正美

    口腔衛生学会雑誌   66 ( 2 )   245 - 245   2016年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 塩化セチルピリジニウム配合トローチ剤の口臭予防効果

    水谷 慎介, 友藤 孝明, 片岡 広太, 川端 勇也, 江國 大輔, 森田 学

    日本口臭学会会誌   7 ( 1 )   54 - 55   2016年4月

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    記述言語:日本語   出版者・発行元:日本口臭学会  

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  • Relationship Between Prehypertension/Hypertension and Periodontal Disease: A Prospective Cohort Study 国際誌

    Yuya Kawabata, Daisuke Ekuni, Hisataka Miyai, Kota Kataoka, Mayu Yamane, Shinsuke Mizutani, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

    AMERICAN JOURNAL OF HYPERTENSION   29 ( 3 )   388 - 396   2016年3月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    BACKGROUND
    Most cross-sectional studies have found a significant positive relationship between periodontal disease and prehypertension/hypertension. However, these studies had limitations and there are few prospective cohort studies in young adults. The purpose of this prospective cohort study was to investigate whether periodontal disease was related to prehypertension/hypertension in Japanese university students.
    METHODS
    Students (n = 2,588), who underwent health examinations before entering university and before graduation, were included in the analysis. The association between periodontal disease such as the percentage of bleeding on probing (BOP) and community periodontal index (CPI) scores, and change in blood pressure status was determined.
    RESULTS
    At the reexamination, the numbers of participants with prehypertension (systolic blood pressure 120-139 mm Hg or diastolic blood pressure 80-89 mm Hg) and hypertension (&gt;= 140/90 mm Hg) were 882 (34.1%) and 109 (4.2%), respectively. In a logistic regression model, the risk of hypertension was significantly associated with male (odds ratio (OR): 6.31; 95% confidence interval (CI): 2.63-15.13; P &lt; 0.001), no habitual physical activity at baseline (OR: 2.90; 95% CI: 1.56-5.38; P &lt; 0.01) and periodontal disease defined as the presence of both probing pocket depth (PPD) &gt;= 4 m and BOP &gt;= 30% at baseline (OR: 2.74; 95% CI: 1.19-6.29; P = 0.02) in participants with prehypertension at baseline. On the other hand, the risk of prehypertension was not associated with presence of periodontal disease (OR: 0.93; 95% CI: 0.51-1.70; P = 0.82).
    CONCLUSION
    In the short-term prospective cohort study, a significant association between presence of periodontal disease and hypertension was observed in Japanese university students.

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  • Association between Knowledge about Comprehensive Food Education and Increase in Dental Caries in Japanese University Students: A Prospective Cohort Study 国際誌

    Muneyoshi Kunitomo, Daisuke Ekuni, Shinsuke Mizutani, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Mayu Yamane, Kota Kataoka, Ayano Taniguchi-Tabata, Hirofumi Mizuno, Hisataka Miyai, Yoshiaki Iwasaki, Manabu Morita

    NUTRIENTS   8 ( 3 )   114 - 114   2016年3月

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    記述言語:英語   出版者・発行元:MDPI AG  

    In Japan, comprehensive food education (shokuiku) programs are carried out with the aim of improving dietary practices and thereby reducing the incidence of lifestyle-related diseases, including dental caries. The purpose of this prospective cohort study was to investigate the association between knowledge about shokuiku and the increase in dental caries among Japanese university students who had attended a shokuiku program while in junior/senior high school. A total of 562 students volunteered to undergo oral examinations over a three-year follow-up period, during which the number of cases of dental caries were recorded. Additional information was collected using a questionnaire survey regarding knowledge about shokuiku, dietary habits, and oral health behaviors. In logistic regression analysis, males who lacked knowledge about shokuiku had significantly higher odds for dental caries than those who did not (odds ratio (OR), 2.00; 95% confidence interval (CI), 1.12-3.58; p = 0.019). On the other hand, among females, those who frequently consumed sugar-sweetened soft drinks had significantly higher odds for dental caries than those who did not (OR, 1.89; 95% CI, 1.05-3.42; p = 0.035). These results suggest that having no knowledge about shokuiku is associated with a risk of increase in dental caries in Japanese male university students.

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  • Relationship Between Prehypertension/Hypertension and Periodontal Disease: A Prospective Cohort Study 国際誌

    Yuya Kawabata, Daisuke Ekuni, Hisataka Miyai, Kota Kataoka, Mayu Yamane, Shinsuke Mizutani, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

    AMERICAN JOURNAL OF HYPERTENSION   29 ( 3 )   388 - 396   2016年3月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    BACKGROUND
    Most cross-sectional studies have found a significant positive relationship between periodontal disease and prehypertension/hypertension. However, these studies had limitations and there are few prospective cohort studies in young adults. The purpose of this prospective cohort study was to investigate whether periodontal disease was related to prehypertension/hypertension in Japanese university students.
    METHODS
    Students (n = 2,588), who underwent health examinations before entering university and before graduation, were included in the analysis. The association between periodontal disease such as the percentage of bleeding on probing (BOP) and community periodontal index (CPI) scores, and change in blood pressure status was determined.
    RESULTS
    At the reexamination, the numbers of participants with prehypertension (systolic blood pressure 120-139 mm Hg or diastolic blood pressure 80-89 mm Hg) and hypertension (&gt;= 140/90 mm Hg) were 882 (34.1%) and 109 (4.2%), respectively. In a logistic regression model, the risk of hypertension was significantly associated with male (odds ratio (OR): 6.31; 95% confidence interval (CI): 2.63-15.13; P &lt; 0.001), no habitual physical activity at baseline (OR: 2.90; 95% CI: 1.56-5.38; P &lt; 0.01) and periodontal disease defined as the presence of both probing pocket depth (PPD) &gt;= 4 m and BOP &gt;= 30% at baseline (OR: 2.74; 95% CI: 1.19-6.29; P = 0.02) in participants with prehypertension at baseline. On the other hand, the risk of prehypertension was not associated with presence of periodontal disease (OR: 0.93; 95% CI: 0.51-1.70; P = 0.82).
    CONCLUSION
    In the short-term prospective cohort study, a significant association between presence of periodontal disease and hypertension was observed in Japanese university students.

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  • 口腔ケアの感染症予防・治療への有効性 上部消化癌患者と健常者における舌苔と口腔内アセトアルデヒド濃度との関連

    山中 玲子, 横井 彩, 丸山 貴之, 江國 大輔, 森田 学

    感染症学雑誌   90 ( 2 )   168 - 169   2016年3月

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    記述言語:日本語   出版者・発行元:(一社)日本感染症学会  

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  • 口腔ケアの感染症予防・治療への有効性 上部消化癌患者と健常者における舌苔と口腔内アセトアルデヒド濃度との関連

    山中 玲子, 横井 彩, 丸山 貴之, 江國 大輔, 森田 学

    日本化学療法学会雑誌   64 ( 2 )   314 - 315   2016年3月

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    記述言語:日本語   出版者・発行元:(公社)日本化学療法学会  

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  • Type D personality and periodontal disease in university students: A prospective cohort study.

    Mizutani S, Ekuni D, Yamane-Takeuchi M, Azuma T, Taniguchi-Tabata A, Tomofuji T, Iwasaki Y, Morita M

    J Health Psychol   1 - 9   2016年

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  • 造血幹細胞移植後に起こる口腔粘膜上細菌叢の変化-PCR-DGGE法を用いた解析

    室美里, 室美里, 曽我賢彦, 片岡広太, 江國大輔, 飯田征二, 森田学

    日本造血細胞移植学会総会プログラム・抄録集   38th   2016年

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  • 大学生における自覚的口腔健康観と口腔内の臨床所見・口腔保健行動との関連

    山根 真由, 江國 大輔, 友藤 孝明, 森田 学

    日本公衆衛生学会総会抄録集   74回   442 - 442   2015年10月

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  • MicroRNAs in Salivary Exosome as Potential Biomarkers of Aging 国際誌

    Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Takayuki Maruyama, Toshiki Yoneda, Yuya Kawabata, Hirofumi Mizuno, Hisataka Miyai, Muneyoshi Kunitomo, Manabu Morita

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   16 ( 9 )   21294 - 21309   2015年9月

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    記述言語:英語   出版者・発行元:MDPI AG  

    The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.

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  • Gingival condition and tooth-brushing behavior after alcohol consumption

    S. Mizutani, D. Ekuni, T. Tomofuji, M. Yamane, T. Azuma, Y. Iwasaki, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   50 ( 4 )   494 - 499   2015年8月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Background and ObjectiveVarious studies have reported the relationship between alcohol consumption and gingival condition. However, they focus on the direct effects of alcohol consumption or alcohol sensitivity on gingival condition, and it is unclear how oral health behaviors relate these relationships. The aims of this study were to assess the inter-relationships between gingival condition, tooth-brushing behavior after drinking alcohol and alcohol sensitivity in university students who drink more than once per week on average.
    Material and MethodsA total of 808 students (541 males, 267 females) that habitually consume alcohol were analyzed. The disease activity of gingival condition was assessed as the percentage of bleeding on probing (%BOP). Additional information regarding alcohol sensitivity and oral health behaviors, including tooth-brushing behavior after drinking, were also collected.
    ResultsThirteen percent of the current participants reported neglecting tooth-brushing after drinking, and their alcohol consumption was higher than those who did not neglect tooth-brushing. Logistic regression analysis showed that high %BOP (%BOP 20) was associated with male (OR=1.53; 95% CI, 1.01-2.33), neglect of tooth-brushing after drinking (OR=2.60; 95% CI, 1.20-5.61) and debris index (OR=8.38; 95% CI, 4.24-16.60) in participants with low alcohol sensitivity. In participants with high alcohol sensitivity, high %BOP was associated with debris index (OR=7.60; 95% CI, 3.12-18.51), but not with any oral health behaviors.
    ConclusionsThe study revealed that alcohol consumption was indirectly related to gingival disease activity through the neglect of tooth-brushing after drinking alcohol in university students with low alcohol sensitivity.

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  • Relationship between Acute Phase of Chronic Periodontitis and Meteorological Factors in the Maintenance Phase of Periodontal Treatment: A Pilot Study 国際誌

    Noriko Takeuchi, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH   12 ( 8 )   9119 - 9130   2015年8月

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    記述言語:英語   出版者・発行元:MDPI AG  

    The acute phase of chronic periodontitis may occur even in patients during supportive periodontal therapy. However, the details are not fully understood. Since the natural environment, including meteorology affects human health, we hypothesized that weather conditions may affect occurrence of acute phase of chronic periodontitis. The aim of this study was to investigate the relationship between weather conditions and acute phase of chronic periodontitis in patients under supportive periodontal therapy. Patients who were diagnosed with acute phase of chronic periodontitis under supportive periodontal therapy during 2011-2013 were selected for this study. We performed oral examinations and collected questionnaires and meteorological data. Of 369 patients who experienced acute phase of chronic periodontitis, 153 had acute phase of chronic periodontitis without direct-triggered episodes. When using the autoregressive integrated moving average model of time-series analysis, the independent covariant of maximum hourly range of barometric pressure, maximum hourly range of temperature, and maximum daily wind speed were significantly associated with occurrence of acute phase of chronic periodontitis (p &lt; 0.05), and 3.1% of the variations in these occurrence over the study period were explained by these factors. Meteorological variables may predict occurrence of acute phase of chronic periodontitis.

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  • Impact of Oral Commensal Bacteria on Degradation of Periodontal Connective Tissue in Mice 国際誌

    Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Manabu Morita, Yoshihiro Shimazaki, Richard P. Darveau

    JOURNAL OF PERIODONTOLOGY   86 ( 7 )   899 - 905   2015年7月

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    記述言語:英語   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Innate and adaptive immunosurveillance mechanisms in response to the normal commensal bacteria can affect periodontal innate defense status. However, it is still unclear how commensal bacteria contribute to the inflammatory responses of junctional epithelium (JE) and periodontal connective tissue (PCT). The aim of the present study is to investigate the contribution of commensal bacteria on inflammatory responses in JE and PCT in mice.
    Methods: The periodontal tissue of germ-free (GF) and specific-pathogen-free (SPF) mice were compared at age 11 to 12 weeks (n = 6 per group). In this study, the number of neutrophils and expression of intercellular adhesion molecule (ICAM)-1, fibroblast growth factor receptor (FGFR)-1, matrix metalloproteinase (MMP)-1, and MMP-8 within the JE and the PCT are evaluated. The collagen density was also determined in PCT stained with picrosirius red (PSR). PSR staining combined with or without polarized light microscopy has been used to assess the organization and maturation of collagen matrix.
    Results: In the present findings, the area of JE in SPF mice was significantly greater than that in GF mice (P &lt;0.05). In addition, the JE and PCT in SPF mice showed greater migration of neutrophils and higher expression of ICAM-1, FGFR-1, MMP-1, and MMP-8 than those in GF mice (P &lt;0.05). Furthermore, the density of collagen in PCT in SPF mice was lower compared to GF mice (P &lt;0.05).
    Conclusion: These results indicate that commensal bacteria induced a low-grade inflammatory state in JE and that such conditions may contribute to degradation of collagen in PCT in mice.

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  • Structural equation modeling to assess gender differences in the relationship between psychological symptoms and dental visits after dental check-ups for university students 国際誌

    Shinsuke Mizutani, Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

    ACTA ODONTOLOGICA SCANDINAVICA   73 ( 5 )   368 - 374   2015年7月

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    記述言語:英語   出版者・発行元:INFORMA HEALTHCARE  

    Objective. Some studies have shown a relationship between psychological symptoms and oral health behaviors. However, it is unknown whether gender differences affect the relationship between psychological symptoms and oral health behaviors. In addition, gender differences in the relationship between dental anxiety and dental visits for treatment or regular check-up are unclear. The objective of the present study was to explain the relationships among gender differences, psychological symptoms, oral health behaviors, dental anxiety and 'expectation of dental visit', evaluated as 'dental visits when treatments are recommended' in university students. Materials and methods. A total of 607 students (311 males, 296 females) aged 18-38 years old were examined. The information was collected via questionnaire regarding gender, psychological symptoms and oral health behaviors. Psychological symptoms were assessed using the Hopkins Symptom Checklist. Structural equation modeling was used to test pathways from these factors to 'expectation of dental visit'. Multiple-group modeling was also conducted to test for gender differences. Results. Psychological symptoms were related to low expectation of dental visit in females, but there was no such relationship in males. Oral health behaviors were related to expectation of dental visit in both genders. Conclusions. Psychological symptoms were directly related to expectation of dental visit in females and oral health behaviors were related to expectation of dental visit in both genders. To promote dental visits after dental check-ups at school, it might be necessary to improve oral health behaviors in both genders and to evaluate psychological symptoms, especially in females.

    DOI: 10.3109/00016357.2014.968870

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  • Association Between Self-Reported Bruxism and Malocclusion in University Students: A Cross-Sectional Study

    Kota Kataoka, Daisuke Ekuni, Shinsuke Mizutani, Takaaki Tomofuji, Tetsuji Azuma, Mayu Yamane, Yuya Kawabata, Yoshiaki Iwasaki, Manabu Morita

    JOURNAL OF EPIDEMIOLOGY   25 ( 6 )   423 - 430   2015年6月

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    記述言語:英語   出版者・発行元:JAPAN EPIDEMIOLOGICAL ASSOC  

    Objectives: Bruxism can result in temporomandibular disorders, oral pain, and tooth wear. However, it is unclear whether bruxism affects malocclusion. The aim of this study was to examine the association between self-reported bruxism and malocclusion in university students.
    Methods: Students (n = 1503; 896 men and 607 women) aged 18 and 19 years were examined. Malocclusion was defined using a modified version of the Index of Orthodontic Treatment Need. The presence of buccal mucosa ridging, tooth wear, dental impression on the tongue, palatal/mandibular torus, and the number of teeth present were recorded, as well as body mass index (BMI). Additional information regarding gender, awareness of bruxism, orthodontic treatment, and oral habits was collected via questionnaire.
    Results: The proportion of students with malocclusion was 32% (n = 481). The awareness of clenching in males with malocclusion was significantly higher than in those with normal occlusion (chi square test, P &lt; 0.01). According to logistic regression analysis, the probability of malocclusion was significantly associated with awareness of clenching (odds ratio [OR] 2.19; 95% confidence interval [CI], 1.22-3.93) and underweight (BMI &lt; 18.5 kg/m(2)) (OR 1.89; 95% CI, 1.31-2.71) in males but not in females. In subgroup analyses, the probability of crowding was also significantly associated with awareness of clenching and underweight (P &lt; 0.01) in males.
    Conclusions: Awareness of clenching and underweight were related to malocclusion (crowding) in university male students.

    DOI: 10.2188/jea.JE20140180

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  • 多職種連携の中で専門的口腔衛生管理を行い、経口摂取を継続しながら化学放射線治療が完遂できた一症例

    志茂 加代子, 丸山 貴之, 横井 彩, 山中 玲子, 三浦 留美, 江國 大輔, 森田 学

    口腔衛生学会雑誌   65 ( 2 )   187 - 187   2015年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 2型糖尿病患者における非外科的歯周病治療の効果の検討

    水野 裕文, 江國 大輔, 米田 俊樹, 片岡 広太, 川端 勇也, 水谷 慎介, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   65 ( 2 )   186 - 186   2015年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 大学生における第二大臼歯の埋伏が第一大臼歯の歯周状態に与える影響

    宮井 久敬, 江國 大輔, 水谷 慎介, 東 哲司, 片岡 広太, 山根 真由, 水野 裕文, 國友 宗義, 谷口 綾乃, 大森 智栄, 森田 学

    口腔衛生学会雑誌   65 ( 2 )   180 - 180   2015年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 還元型コエンザイムQ10の抜歯窩創傷治癒への効果の検討

    米田 俊樹, 友藤 孝明, 川端 勇也, 江國 大輔, 東 哲司, 片岡 広太, 國友 宗義, 森田 学

    口腔衛生学会雑誌   65 ( 3 )   312 - 312   2015年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 天然植物由来成分配合歯磨剤のラット歯周炎に対する影響

    友藤 孝明, 江國 大輔, 東 哲司, 米田 俊樹, 片岡 広太, 川端 勇也, 山根 真由, 森田 学

    日本歯周病学会会誌   57 ( 春季特別 )   126 - 126   2015年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • Relationship between xerostomia and gingival condition in young adults

    S. Mizutani, D. Ekuni, T. Tomofuji, T. Azuma, K. Kataoka, M. Yamane, Y. Iwasaki, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   50 ( 1 )   74 - 79   2015年2月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Background and ObjectiveXerostomia is a subjective symptom of dryness in the mouth. Although a correlation between xerostomia and oral conditions in the elderly has been reported, there are few such studies in the young adults. The aim of this study was to examine the relationship of xerostomia with the gingival condition in university students.
    Material and MethodsA total of 2077 students (1202 male subjects and 875 female subjects), 18-24years of age, were examined. The disease activity and severity of the gingival condition were assessed as the percentage of teeth with bleeding on probing (%BOP) and the presence of teeth with probing pocket depth of 4mm, respectively. Additional information on xerostomia, oral health behaviors, coffee/tea intake and nasal congestion was collected via a questionnaire. Path analysis was used to test pathways from xerostomia to the gingival condition.
    ResultsOne-hundred and eighty-three (8.8%) students responded that their mouths frequently or always felt dry. Xerostomia was related to %BOP and dental plaque formation, but was not related to the presence of probing pocket depth 4mm. In the structural model, xerostomia was related to dental plaque formation (p&lt;0.01), and a lower level of dental plaque formation was associated with a lower %BOP. Xerostomia was associated with coffee/tea intake (p&lt;0.01) and nasal congestion (p&lt;0.001).
    ConclusionXerostomia was indirectly related to gingival disease activity through the accumulation of dental plaque. Nasal congestion and coffee/tea intake also affected xerostomia. These findings suggest that xerostomia should be considered in screening for gingivitis risk in young adults.

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  • Relationship between acetaldehyde concentration in mouth air and tongue coating volume 国際誌

    Aya Yokoi, Takayuki Maruyama, Reiko Yamanaka, Daisuke Ekuni, Takaaki Tomofuji, Haruhiko Kashiwazaki, Yutaka Yamazaki, Manabu Morita

    JOURNAL OF APPLIED ORAL SCIENCE   23 ( 1 )   64 - 70   2015年1月

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    記述言語:英語   出版者・発行元:UNIV SAO PAULO FAC ODONTOLOGIA BAURU  

    Objective: Acetaldehyde is the first metabolite of ethanol and is produced in the epithelium by mucosal ALDH, while higher levels are derived from microbial oxidation of ethanol by oral microflora such as Candida species. However, it is uncertain whether acetaldehyde concentration in human breath is related to oral condition or local production of acetaldehyde by oral microflora. The aim of this pilot study was to investigate the relationship between physiological acetaldehyde concentration and oral condition in healthy volunteers. Material and Methods: Sixty-five volunteers (51 males and 14 females, aged from 20 to 87 years old) participated in the present study. Acetaldehyde concentration in mouth air was measured using a portable monitor. Oral examination, detection of oral Candida species and assessment of alcohol sensitivity were performed. Results: Acetaldehyde concentration [median (25%, 75%)] in mouth air was 170.7 (73.5, 306.3) ppb. Acetaldehyde concentration in participants with a tongue coating status score of 3 was significantly higher than in those with a score of 1 (p&lt;0.017). After removing tongue coating, acetaldehyde concentration decreased significantly (p&lt;0.05). Acetaldehyde concentration was not correlated with other clinical parameters, presence of Candida species, smoking status or alcohol sensitivity. Conclusion: Physiological acetaldehyde concentration in mouth air was associated with tongue coating volume.

    DOI: 10.1590/1678-775720140223

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  • Self-efficacy and progression of periodontal disease: A prospective cohort study. 国際誌

    Azuma T, Yamane M, Ekuni D, Kawabata Y, Kataoka K, Kasuyama K, Maruyama T, Tomofuji T, Morita M

    J Clin Periodontol.   42 ( 12 )   1083 - 1089   2015年

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  • 歯周疾患と糖尿病等との関係に着目した歯科保健指導方法の開発等に関する研究

    森田学, 和田淳, 友藤孝明, 江國大輔, 安藤雄一, 荻野景規

    厚生労働科学研究費補助金 循環器疾患・糖尿病等生活習慣病対策総合研事業 平成26年度 総括・分担研究報告書   2015年

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  • Serum Oxidative/anti-oxidative Stress Balance Is Dysregulated in Potentially Pulmonary Hypertensive Patients with Liver Cirrhosis: A Case Control Study

    Masako Terao, Akinobu Takaki, Takayuki Maruyama, Hiroki Oe, Tetsuya Yasunaka, Naofumi Tamaki, Kazufumi Nakamura, Takaaki Tomofuji, Takahito Yagi, Hiroshi Sadamori, Yuzo Umeda, Susumu Shinoura, Ryuichi Yoshida, Kazuhiro Nouso, Daisuke Ekuni, Kazuko Koike, Fusao Ikeda, Hidenori Shiraha, Manabu Morita, Hiroshi Ito, Toshiyoshi Fujiwara, Kazuhide Yamamoto

    INTERNAL MEDICINE   54 ( 22 )   2815 - 2826   2015年

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    記述言語:英語   出版者・発行元:JAPAN SOC INTERNAL MEDICINE  

    Objective Hepatopulmonary syndrome (HPS) is characterized by vascular dilatation and hyperdynamic circulation, while portopulmonary hypertension (POPH) is characterized by vasoconstriction with fibrous obliteration of the vascular bed. Vasoactive molecules such as nitric oxide (NO) are candidate factors for cirrhotic complications associated with these diseases. However, oxidative stress balance is not well characterized in HPS and POPH. The present objective is to investigate the oxidative stress and anti-oxidative stress balance and NO pathway balance in patients with potential HPS and POPH.
    Methods We recruited patients with decompensated cirrhosis (n = 69) admitted to our hospital as liver transplantation candidates. Patients exhibiting partial pressure of oxygen lower than 80 mmHg and alveolararterial oxygen gradient (AaDO(2)) &gt;= 15 mmHg were categorized as potentially having HPS (23 of 69 patients). Patients exhibiting a tricuspid regurgitation pressure gradient &gt;= 25 mmHg were categorized as potentially having POPH (29 of 61 patients). Serum reactive oxygen metabolites were measured and anti-oxidative OXY-adsorbent test (OXY) were performed, and the balance of these tests was defined as the oxidative index. The correlation between these values and the clinical characteristics of the patients were assessed in a cross-sectional study.
    Results Potential HPS patients exhibited no correlation with oxidative stress markers. Potential POPH patients exhibited lower OXY (p = 0.037) and higher oxidative index values (p = 0.001). Additionally, the vascular NO synthase enzyme inhibiting protein, asymmetric dimethylarginine, was higher in potential POPH patients (p = 0.049). The potential POPH patients exhibited elevated AaDO(2), suggesting the presence of pulmonary shunting.
    Conclusion Potential POPH patients exhibited elevated oxidative stress with decreased anti-oxidative function accompanied by inhibited NO production. Anti-oxidants represent a candidate treatment for potential POPH patients.

    DOI: 10.2169/internalmedicine.54.4889

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  • 大学生における早食いと肥満の関係

    山根真由, 江國大輔, 森田 学

    厚生の指標   62 ( 7 )   7 - 11   2015年

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    記述言語:日本語   出版者・発行元:厚生労働統計協会  

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  • 塩化セチルピリジニウム配合トローチ剤の口臭抑制効果

    友藤 孝明, 片岡 広太, 川端 勇也, 水谷 慎介, 江國 大輔, 森田 学

    日口臭誌   6 ( 1 )   15 - 20   2015年

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    記述言語:日本語   出版者・発行元:日本口臭学会  

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  • 歯周病メインテナンス期患者に対する血液検査による生活習慣病スクリーニングの検討

    町田達哉, 友藤孝明, 江國大輔, 東 哲司, 竹内倫子, 丸山貴之, 川端勇也, 森田 学

    口腔衛生会誌   65 ( 4 )   354 - 361   2015年

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    記述言語:日本語   出版者・発行元:有限責任中間法人日本口腔衛生学会  

    生活習慣病予防はわが国喫緊の課題である.生活習慣病スクリーニングを受ける機会の提供,生活習慣病に関する情報提供,および,医療機関への適切な受診勧奨等が生活習慣病予防のために重要である.本研究では,これらを実施する場の一つとして歯科を捉え,その可能性を検討することを目的とした.簡易な血液検査による生活習慣病スクリーニングを歯周病メインテナンス期患者に行い,分析した.2014年1月から7月に岡山大学病院予防歯科外来を受診した歯周病メインテナンス期患者63名(男性22名,女性41名,平均年齢65.9±6.7歳)を対象に,自己記入式質問紙調査と指尖血の採取を行い,肝疾患,高脂血症(脂質異常症),腎疾患,痛風,糖尿病前症,および糖尿病の疑いの有無を判定した.過去1年間の医療機関受診歴を53名(84.1%)が有していた.過去1年間に肝疾患,高脂血症,腎疾患,痛風,および糖尿病の診断のある者は,それぞれ3名,14名,2名,1名,3名おり,各々の分析から除外された.血液検査の結果,肝疾患,高脂血症,腎疾患,痛風,糖尿病前症,および糖尿病の疑いは,それぞれ対象者の16.7%,34.7%,16.4%,1.6%,46.7%,および6.7%に認められた.医療機関受診歴を有する者の多さに関わらず,生活習慣病予防が必要と思われる者の割合は十分多かった.歯科の場において,簡易な生活習慣病スクリーニングを行い,医療機関への受診勧奨等を行う流れを作ることで,国民の生活習慣病予防の一端を担えるかもしれない.

    DOI: 10.5834/jdh.65.4_354

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  • Drinking Hydrogen-Rich Water Has Additive Effects on Non-Surgical Periodontal Treatment of Improving Periodontitis: A Pilot Study. 国際誌

    Azuma T, Yamane M, Ekuni D, Kawabata Y, Kataoka K, Kasuyama K, Maruyama T, Tomofuji T, Morita M

    antioxidants   4 ( 3 )   513 - 22   2015年

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  • 口腔内アセトアルデヒド濃度と舌苔スコアとの関係

    横井 彩, 江國大輔, 森田 学

    日本歯科評論   75 ( 7 )   139 - 141   2015年

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    記述言語:日本語   出版者・発行元:(株)ヒョーロン・パブリッシャーズ  

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  • 歯周組織の加齢変化に対する口腔常在菌の影響

    入江浩一郎, 入江浩一郎, 友藤孝明, 江國大輔, 小林周一郎, 菊池毅, 内田瑶子, 福原大樹, 三谷章雄, 嶋崎義浩, 森田学

    日本歯周病学会会誌(Web)   57   2015年

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  • Application of Coenzyme Q10 for Accelerating Soft Tissue Wound Healing after Tooth Extraction in Rats 国際誌

    Toshiki Yoneda, Takaaki Tomofuji, Yuya Kawabata, Daisuke Ekuni, Tetsuji Azuma, Kota Kataoka, Muneyoshi Kunitomo, Manabu Morita

    NUTRIENTS   6 ( 12 )   5756 - 5769   2014年12月

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    記述言語:英語   出版者・発行元:MDPI AG  

    Accelerating wound healing after tooth extraction is beneficial in dental treatment. Application of antioxidants, such as reduced coenzyme Q10 (rCoQ10), may promote wound healing after tooth extraction. In this study, we examined the effects of topical application of rCoQ10 on wound healing after tooth extraction in rats. After maxillary first molars were extracted, male Fischer 344 rats (8 weeks old) (n = 27) received topical application of ointment containing 5% rCoQ10 (experimental group) or control ointment (control group) to the sockets for 3 or 8 days (n = 6-7/group). At 3 days after extraction, the experimental group showed higher collagen density and lower numbers of polymorphonuclear leukocytes in the upper part of socket, as compared to the control group (p &lt; 0.05). Gene expression of interleukin-1, tumor necrosis factor-alpha and nuclear factor-kappa B were also lower in the experimental group than in the control group (p &lt; 0.05). At 8 days after tooth extraction, there were no significant differences in collagen density, number of polymorphonuclear leukocytes and bone fill between the groups. Our results suggest that topical application of rCoQ10 promotes wound healing in the soft tissue of the alveolar socket, but that rCoQ10 has a limited effect on bone remodeling in rats.

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  • 近年における永久歯の萠出年齢の推移

    竹内 倫子, 友藤 孝明, 江國 大輔, 山中 玲子, 古田 美智子, 山本 龍生, 恒石 美登里, 森田 学

    日本公衆衛生学会総会抄録集   73回   419 - 419   2014年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • Relationships Between Eating Quickly and Weight Gain in Japanese University Students: A Longitudinal Study 国際誌

    Mayu Yamane, Daisuke Ekuni, Shinsuke Mizutani, Kota Kataoka, Masami Sakumoto-Kataoka, Yuya Kawabata, Chie Omori, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

    OBESITY   22 ( 10 )   2262 - 2266   2014年10月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    ObjectiveMany cross-sectional studies have reported a relationship between overweight/obesity and eating quickly, but there have been few longitudinal studies to address this relationship in younger populations. The purpose of this prospective longitudinal study was to investigate whether eating quickly was related to being overweight in Japanese university students.
    MethodsOf 1,396 students who underwent a general examination and completed questionnaires at the start of university and before graduation, 1,314 students (676 male and 638 female) of normal body composition [body mass index (BMI)&lt;25 kg m(-2)] at baseline were included in the analysis. The questionnaires included speed of eating and other lifestyle factors. After a 3-year follow-up, the students whose BMIs were25 kg m(-2) were defined as overweight.
    ResultsIn this study, 38 participants (2.9%) became overweight. In the logistic regression analysis, the risk of being overweight was increased in males [adjusted odds ratio (OR): 2.77; 95% confidence interval (CI): 1.33-5.79; P&lt;0.01] and in those who ate quickly at baseline (OR: 4.40; 95% CI: 2.22-8.75; P&lt;0.001).
    ConclusionsEating quickly may predict risk of being overweight in Japanese university students.

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  • Severe Periodontitis Is Inversely Associated with Coffee Consumption in the Maintenance Phase of Periodontal Treatment 国際誌

    Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Noriko Takeuchi, Takayuki Maruyama, Shinsuke Mizutani, Kota Kataoka, Yuya Kawabata, Manabu Morita

    NUTRIENTS   6 ( 10 )   4476 - 4490   2014年10月

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    記述言語:英語   出版者・発行元:MDPI AG  

    This cross-sectional study addressed the relationship between coffee consumption and periodontitis in patients during the maintenance phase of periodontal treatment. A total of 414 periodontitis patients in the maintenance phase of periodontal treatment completed a questionnaire including items related to coffee intake and underwent periodontal examination. Logistic regression analysis showed that presence of moderate/severe periodontitis was correlated with presence of hypertension (Odds Ratio (OR) = 1.99, p &lt; 0.05), smoking (former, OR = 5.63, p &lt; 0.01; current, OR = 6.81, p = 0.076), number of teeth present (OR = 0.89, p &lt; 0.001), plaque control record &gt;= 20% (OR = 1.88, p &lt; 0.05), and duration of maintenance phase (OR = 1.07, p &lt; 0.01). On the other hand, presence of severe periodontitis was correlated with smoking (former, OR = 1.35, p = 0.501; current, OR = 3.98, p &lt; 0.05), coffee consumption (&gt;= 1 cup/day, OR = 0.55, p &lt; 0.05), number of teeth present (OR = 0.95, p &lt; 0.05), and bleeding on probing &gt;= 20% (OR = 3.67, p &lt; 0.001). There appears to be an inverse association between coffee consumption (&gt;= 1 cup/day) and prevalence of severe periodontitis in the maintenance phase of periodontal treatment.

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  • 若年者の早食いは肥満のリスクになる

    山根 真由, 江國 大輔, 友藤 孝明, 森田 学

    日本公衆衛生学会総会抄録集   73回   209 - 209   2014年10月

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    記述言語:日本語   出版者・発行元:日本公衆衛生学会  

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  • Occlusal disharmony accelerates the initiation of atherosclerosis in apoE knockout rats 国際誌

    Daisuke Ekuni, Toshiki Yoneda, Yasumasa Endo, Kenta Kasuyama, Koichiro Irie, Shinsuke Mizutani, Tetsuji Azuma, Takaaki Tomofuji, Manabu Morita

    LIPIDS IN HEALTH AND DISEASE   13 ( 1 )   144 - 144   2014年9月

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    記述言語:英語   出版者・発行元:BIOMED CENTRAL LTD  

    Background: Psychosocial stress is one of the risk factors for atherosclerosis. As occlusal disharmony induces psychological stress, we hypothesized that psychological stress by occlusal disharmony accelerates atherosclerosis. The aim of this study was to investigate the effects of occlusal disharmony on the initiation of atherosclerosis in apolipoprotein E (apoE) knockout rats.
    Methods: Fourteen male apoE-knockout rats (age; 8 weeks) (Sprague-Dawley strain background) were divided into two groups of seven rats: the occlusal disharmony group and the no treatment (control) group. In the occlusal disharmony group, the maxillary molar cusps were cut off for the 8-week experimental period.
    Results: In the occlusal disharmony group, the percentages of the area of total aortic lumen occupied by plaques and lipid were significantly higher than those in the control group (p &lt; 0.05, t-test). The occlusal disharmony group also showed significantly higher serum levels of very low-density lipoprotein-cholesterol (VLDL) and low-density lipoprotein-cholesterol (LDL), plasma levels of corticosterone (1.9, 1.3 and 1.3 times, respectively), higher aortic protein expression levels of vascular cell adhesion molecule-1 (VCAM1) and intercellular adhesion molecule-1 (ICAM1) (1.5 and 1.4 times, respectively), and higher aortic gene expression of levels of VCAM1 and Toll-like receptor 4 (TLR4) (1.9 and 4.3 times, respectively), as compared to the control group (p &lt; 0.05). However, there were no significant differences in serum levels of oxidized LDL, reactive oxygen metabolites and C-reactive protein between the two groups.
    Conclusion: In apoE knockout rats, occlusal disharmony may induce VCAM1, ICAM1 and TLR4 expression and accelerate the initiation of atherosclerosis.

    DOI: 10.1186/1476-511X-13-144

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  • 分子イメージングの可能性 歯学分野での応用と今後の展望

    江國 大輔

    Journal of Oral Biosciences Supplement   2014   104 - 104   2014年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • Relationship between increases in BMI and changes in periodontal status: a prospective cohort study 国際誌

    Daisuke Ekuni, Shinsuke Mizutani, Azusa Kojima, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Toshiki Yoneda, Michiko Furuta, Nobuoki Eshima, Yoshiaki Iwasaki, Manabu Morita

    JOURNAL OF CLINICAL PERIODONTOLOGY   41 ( 8 )   772 - 778   2014年8月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Aim: The purpose of this prospective cohort study was to investigate whether body mass index (BMI) and oral health behaviour are related to changes in periodontal status in Japanese university students.
    Materials and Methods: Students (n = 224) who were interested in receiving oral health examinations before entering university and before graduation were included in the analysis. Subjects were investigated regarding the correlations of oral health behaviours and increases in BMI with the percentage of bleeding on probing (% BOP) and Community Periodontal Index (CPI) scores as indicators of changes in periodontal status.
    Results: The risk of increased % BOP was associated with the non-use of dental floss (adjusted odds ratio [OR]: 3.11; 95% confidence interval [CI]: 1.31-7.37; p &lt; 0.05), whereas the risk of increased CPI score was associated with increases in BMI (OR: 1.95; 95% CI: 1.05-3.65; p &lt; 0.05) and simplified oral hygiene index score (OR: 2.28; 95% CI: 1.23-4.22; p &lt; 0.01).
    Conclusion: Increases in BMI were associated with worsening of periodontal status, defined as increased CPI score in Japanese university students, whereas lack of inter-dental cleaning was associated with exacerbated gingival bleeding.

    DOI: 10.1111/jcpe.12273

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  • Effects of hydrogen-rich water on aging periodontal tissues in rats 国際誌

    Takaaki Tomofuji, Yuya Kawabata, Kenta Kasuyama, Yasumasa Endo, Toshiki Yoneda, Mayu Yamane, Tetsuji Azuma, Daisuke Ekuni, Manabu Morita

    SCIENTIFIC REPORTS   4   5534 - 5534   2014年7月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Oxidative damage is involved in age-related inflammatory reactions. The anti-oxidative effects of hydrogen-rich water suppress oxidative damage, which may aid in inhibiting age-related inflammatory reactions. We investigated the effects of drinking hydrogen-rich water on aging periodontal tissues in healthy rats. Four-month-old male Fischer 344 rats (n = 12) were divided into two groups: the experimental group (hydrogen-rich water treatment) and the control group (distilled water treatment). The rats consumed hydrogen-rich water or distilled water until 16 months of age. The experimental group exhibited lower periodontal oxidative damage at 16 months of age than the control group. Although protein expression of interleukin-1 beta did not differ, gene expression of Nod-like receptor protein 3 inflammasomes was activated in periodontal tissues from the experimental group as compared with the control group. Drinking hydrogen-rich water is proposed to have anti-aging effects on periodontal oxidative damage, but not on inflammatory reactions in healthy rats.

    DOI: 10.1038/srep05534

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  • 若年者における急性上気道炎の発症頻度と智歯萠出状態との関連

    町田 達哉, 水谷 慎介, 片岡 広太, 山根 真由, 丸山 貴之, 東 哲司, 友藤 孝明, 江國 大輔, 森田 学

    口腔衛生学会雑誌   64 ( 4 )   328 - 335   2014年7月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

    口腔状態と気道感染の関連性の議論が近年盛んである。口腔は上気道と接しており、半萠出智歯の存在は、隣在歯との間に清掃困難な空間を形成し、智歯周囲の細菌量を増加させ、上気道を含む周囲組織に影響を与えうる。そこで、対象者を智歯萠出時期である18-20歳の大学生とし、急性上気道炎の発症頻度と智歯萠出状態の関連を検討することとした。2013年岡山大学新入生健康診断の際に歯科健診を任意受診した18-67歳(平均年齢18.5±2.1歳)の2,205名中、非喫煙者でデータ欠損のない18-20歳の1,964名(男性1,130名、女性834名)を分析対象とした。口腔診査を行い口腔清掃度、地域歯周疾患指数、および智歯萠出状態を調べた。自己記入式質問調査により過去1年間の急性上気道炎の発症頻度を尋ね、年間3回以上発症した群と3回未満発症した群の2群に分けた。χ2検定で2群比較を行い、有意な関連を智歯萠出状態と過去1年間の一番奥の歯ぐきの痛みの既往で認めた(P&lt;0.05)。口腔清掃度にも一定の関連性を認めた(P=0.054)。性別、口腔清掃度、智歯萠出状態、過去1年間の一番奥の歯ぐきの痛みの既往を説明変数としたロジスティック回帰分析を行ったところ、萠出智歯なしの者に対し、半萠出智歯を有する者は急性上気道炎を過去1年間に3回以上発症していた者の割合が有意に高かった(P=0.003)。大学生において智歯萠出状態と急性上気道炎の発症頻度に関連性があることが示唆された。(著者抄録)

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    その他リンク: http://search.jamas.or.jp/link/ui/2014337245

  • 某事業所の歯科健診受診者における歯周病の知識がない者の特徴

    山本 龍生, 渕田 慎也, 平田 幸夫, 江國 大輔, 森田 学, 恒石 美登里, 鈴木 洋美, 島村 広海, 安信 忠義, 公衆歯科衛生研究会

    産業衛生学雑誌   56 ( 臨増 )   428 - 428   2014年5月

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    記述言語:日本語   出版者・発行元:(公社)日本産業衛生学会  

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  • 歯周状態と全身性の酸化ストレスとの縦断的な関連

    森田 学, 町田 達哉, 友藤 孝明, 江國 大輔, 山根 真由, 米田 俊樹, 川端 勇也, 片岡 広太

    産業衛生学雑誌   56 ( 臨増 )   429 - 429   2014年5月

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    記述言語:日本語   出版者・発行元:(公社)日本産業衛生学会  

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  • 職域成人における口腔内所見および口腔保健行動とメタボリックシンドロームとの関係

    林 浩範, 江國 大輔, 米田 俊樹, 水谷 慎介, 赤澤 百合子, 森田 学

    産業衛生学雑誌   56 ( 臨増 )   429 - 429   2014年5月

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    記述言語:日本語   出版者・発行元:(公社)日本産業衛生学会  

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  • 若年者における血圧と歯周炎との関連についてのコホート研究

    川端 勇也, 江國 大輔, 友藤 孝明, 東 哲司, 丸山 貴之, 水谷 慎介, 米田 俊樹, 町田 達哉, 片岡 広太, 山根 真由, 佐久本 雅美, 大森 智栄, 森田 学

    口腔衛生学会雑誌   64 ( 2 )   175 - 175   2014年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 口腔常在菌叢による歯周組織への影響

    入江 浩一郎, ダービュ・リチャード, 友藤 孝明, 江國 大輔, 森田 学, 嶋崎 義浩

    日本歯周病学会会誌   56 ( 春季特別 )   120 - 120   2014年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 肥満と歯周病進行との関連

    米田 俊樹, 江國 大輔, 水谷 慎介, 児島 梓, 友藤 孝明, 入江 浩一郎, 東 哲司, 森田 学

    口腔衛生学会雑誌   64 ( 3 )   316 - 316   2014年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 大学生における第二大臼歯の埋伏状況と口腔内の他の因子との関係

    大森 智栄, 江國 大輔, 片岡 広太, 山根 真由, 水谷 慎介, 東 哲司, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   64 ( 3 )   315 - 315   2014年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 若年者における口腔乾燥感と歯周病との関連

    水谷 慎介, 江國 大輔, 友藤 孝明, 東 哲司, 片岡 広太, 山根 真由, 森田 学

    口腔衛生学会雑誌   64 ( 3 )   312 - 312   2014年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 咬合異常によるストレスはアポリポタンパクE欠損ラットの動脈硬化を増悪する

    江國 大輔, 米田 俊樹, 遠藤 康正, 粕山 健太, 入江 浩一郎, 水谷 慎介, 東 哲司, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   64 ( 2 )   230 - 230   2014年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 口腔常在菌に対する宿主防御機構への影響

    入江 浩一郎, 友藤 孝明, 江國 大輔, 森田 学, 嶋崎 義浩

    口腔衛生学会雑誌   64 ( 2 )   181 - 181   2014年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 歯周病による全身の酸化ストレスへの影響 酸化ストレス可視化マウスを用いた検討

    片岡 広太, 江國 大輔, 粕山 健太, 川端 勇也, 山根 真由, 遠藤 康正, 米田 俊樹, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   64 ( 2 )   181 - 181   2014年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • Anti-ageing effects of dentifrices containing anti-oxidative, anti-inflammatory, and anti-bacterial agents (Tomarina®) on gingival collagen degradation in rats.

    Koichiro I, Tomofuji T, Ekuni D, Endo Y, Kasuyama K, Azuma T, Tamaki N, Yoneda T, Morita M

    Arch Oral Biol   59 ( 1 )   60 - 65   2014年1月

  • Factors related to the formation of buccal mucosa ridging in university students 国際誌

    Shinsuke Mizutani, Daisuke Ekuni, Takaaki Tomofuji, Tetsuji Azuma, Koichiro Irie, Tatsuya Machida, Toshiki Yoneda, Yoshiaki Iwasaki, Manabu Morita

    ACTA ODONTOLOGICA SCANDINAVICA   72 ( 1 )   58 - 63   2014年1月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    Objective. Buccal mucosa ridging (BMR) is known as a clinical sign of clenching, which is one of the major manifestations of bruxism. However, there are few reports about the formation of BMR and no data regarding the association between BMR and factors such as malocclusion. The purpose of the current study was to investigate the relationship between BMR and factors such as the number of teeth present, gender, body mass index (BMI), occlusion and clenching habit in university students. Materials and methods. A total of 2101 students (1164 males, 937 females), aged 18-29 years old, were included in the study. BMR and the number of teeth present were recorded and malocclusion was defined using a modified version of the Index of Orthodontic Treatment Need. Additional information regarding gender, clenching habit and BMI was collected via a questionnaire. Results. Forty-six per cent of the subjects had BMR and the prevalence of BMR in females was significantly higher than that of males (chi square test, p &lt; 0.001). According to logistic regression analysis, the probability of BMR was significantly associated with female gender (OR = 1.501, 95% CI = 1.259-1.790, p &lt; 0.001), crowding (OR = 2.102, 95% CI = 1.706-2.590, p &lt; 0.001) and overjet (OR = 0.585, 95% CI = 0.418-0.818, p = 0.002). On the other hand, BMR was not associated with awareness of clenching habit and BMI. Conclusions. Gender, crowding and overjet were related to the formation of BMR in university students. When evaluating BMR as a clinical sign of clenching, one might have to take factors such as gender and crowding into consideration.

    DOI: 10.3109/00016357.2013.797102

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  • Periodontitis and atherosclerosis.

    Ekuni D, Tomofuji T, Morita M

    Springer   2014年

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  • Periodontitis and Atherosclerosis.

    Ekuni D, Tomofuji T, Morita M

    Studies on Periodontal Disease   183 - 195   2014年

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  • Effects of Antioxidants on Periodontal Disease.

    Tomofuji T, Ekuni D, Mizutani S, Morita M

    Studies on Periodontal Disease   279 - 305   2014年

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  • Periodontitis and Liver Diseases.

    Irie K, Ekuni D, Tomofuji T, Morita M

    Studies on Periodontal Disease   197 - 209   2014年

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  • Association between oxidative stress and periodontal diseases in animal model studies

    Morita M, Ekuni D, Tomofuji T

    Springer   2014年

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  • Longitudinal Relationship between Plasma Reactive Oxygen Metabolites and Periodontal Condition in the Maintenance Phase of Periodontal Treatment

    Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Mayu Yamane, Toshiki Yoneda, Yuya Kawabata, Kota Kataoka, Naofumi Tamaki, Manabu Morit

    DISEASE MARKERS   2014   489292 - 489292   2014年

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    記述言語:英語   出版者・発行元:HINDAWI PUBLISHING CORPORATION  

    Aim. The present cohort study describes the longitudinal relationship between plasma oxidative status and periodontitis progression during the maintenance phase of treatment. Materials and Methods. Forty-five patients (mean age 58.8 years) were monitored from 2008 to 2013. Periodontal conditions, including probing pocket depth (PPD) and clinical attachment level (CAL), were recorded. Measurements of plasma reactive oxygen metabolites (ROM) and biologic antioxidant potential (BAP) were performed to evaluate plasma oxidative status. The patients were assigned into 2 groups as low and high plasma ROM level using a cut-off value which was median of plasma ROM level at baseline. Results. In the subjects with low plasma ROM level at baseline, changes in mean CAL were positively correlated with changes in plasma ROM levels, bleeding on probing, and plaque control record, but not with PPD. In the subjects with high plasma ROM at baseline, changes in CAL were significantly associated with only PPD at baseline. On the other hands there were no significant associations between changes in CAL and those in plasma BAP levels. Conclusions. When plasma ROM level in periodontitis patients was low, increases in plasma ROM level were associated with those in CAL during the maintenance phase of treatment.

    DOI: 10.1155/2014/489292

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  • Association Between Oxidative Stress and Periodontal Diseases in Animal Model Studies.

    Morita M, Ekuni D, Tomofuji T

    Studies on Periodontal Disease   33 - 51   2014年

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  • Effects of antioxidants on periodontal disease.

    Tomofuji T, Ekuni D, Mizutani S, Morita M

    Springer   2014年

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  • Effects of Periodontal Therapy on Circulating Oxidative Stress.

    Tomofuji T, Ekuni D, Morita M

    Studies on Periodontal Disease   147 - 156   2014年

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  • Expression of Reactive Oxygen Species in Junctional and Pocket Epithelium.

    Ekuni D, Firth JD, Putnins EE

    Studies on Periodontal Disease   53 - 75   2014年

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  • Effects of periodontal therapy on circulating oxidative stress.

    Tomofuji T, Ekuni D, Morita M

    Springer   2014年

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  • Expression of reactive oxygen species in junctional and pocket epithelium.

    ekuni D, Firth JD, Putnins EE

    Springer   2014年

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  • Periodontitis and liver diseases.

    Irie K, Ekuni D, Tomofuji T, Morita M

    Springer   2014年

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  • アルコール摂取後の口腔保健行動の変化と歯周病との関連

    山根 真由, 江國 大輔, 水谷 慎介, 町田 達哉, 片岡 広大, 丸山 貴之, 森田 学

    岡山歯学会雑誌   32 ( 2 )   87 - 88   2013年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 若年者における風邪の発症頻度と智歯の萠出状態との関連

    町田 達哉, 水谷 慎介, 片岡 広太, 山根 真由, 丸山 貴之, 友藤 孝明, 江國 大輔, 森田 学

    岡山歯学会雑誌   32 ( 2 )   87 - 87   2013年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 咬合異常はラット海馬のベータアミロイドを誘導する

    江國 大輔

    岡山歯学会雑誌   32 ( 2 )   77 - 78   2013年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • Relationships between self-rated oral health, subjective symptoms, oral health behavior and clinical conditions in Japanese university students: a cross-sectional survey at Okayama University 国際誌

    Azusa Kojima, Daisuke Ekuni, Shinsuke Mizutani, Michiko Furuta, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

    BMC ORAL HEALTH   13 ( 1 )   62 - 62   2013年11月

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    記述言語:英語   出版者・発行元:BIOMED CENTRAL LTD  

    Background: Self-rated oral health is a valid and useful summary indicator of overall oral health status and quality of life. However, few studies on perception of oral health have been conducted among Japanese young adults. This study investigated whether oral health behavior, subjective oral symptoms, or clinical oral status were associated with self-rated oral health in Japanese young adults.
    Methods: This cross-sectional survey included 2,087 students (1,183 males, 904 females), aged 18 and 19 years, at Okayama University, Japan. A self-administered questionnaire was distributed and an oral examination was performed.
    Results: In a structural equation modeling analysis, the score of decayed, missing and filled teeth (DMFT) significantly affected self-rated oral health (p &lt;0.05) and the effect size was highest. Malocclusion, subjective symptoms of temporomandibular disorders (TMD) and stomatitis, and poor oral health behavior significantly induced self-rated poor oral health with small effect sizes (p &lt;0.05). Clinical periodontal conditions and Oral Hygiene Index-simplified were not related to self-rated oral health.
    Conclusion: Self-rated oral health was influenced by subjective symptoms of TMD and stomatitis, oral health behavior, the score of DMFT, and malocclusion. The evaluation of these parameters may be a useful approach in routine dental examination to improve self-rated oral health in university students.

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  • Effects of apoE Deficiency and Occlusal Disharmony on Amyloid-Beta Production and Spatial Memory in Rats 国際誌

    Daisuke Ekuni, Yasumasa Endo, Takaaki Tomofuji, Tetsuji Azuma, Koichiro Irie, Kenta Kasuyama, Manabu Morita

    PLoS ONE   8 ( 9 )   e74966   2013年9月

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    Amyloid-β (Aβ) plays a causative role in Alzheimer's disease. Apolipoprotein E (apoE) is involved in Aβ accumulation, whereas occlusal disharmony increases Aβ production in the rat hippocampus. The purpose of the present study was to investigate the effects of apoE deficiency and occlusal disharmony on Aβ production and spatial memory. Wild-type (WT) (n = 12) and apoE-deficient [ApoE(-/-)] (n = 12) rats (Sprague-Dawley
    8 weeks old) were used. These rats were randomly divided into four groups of six rats each: two control (C) groups: WT (C-WT) and ApoE [C-ApoE(-/-)], and two occlusal disharmony (D) groups: WT (D-WT) and ApoE [D-ApoE(-/-)]. The C group received no treatment for 8 weeks. In the D group, the maxillary molar cusps were cut off for 8 weeks. The spatial memory of rats was assessed according to their behavioral performance in a radial arm maze. In both genotypes of rats, significant differences in the reference memory, Aβ42 production, β-secretase expression and plasma corticosterone levels were observed between the C and D groups (P &lt
    0.0125). The levels of Aβ42 and glucocorticoid receptor in the C-ApoE(-/-) group were also significantly higher than those in the C-WT group (P &lt
    0.0125). However, no significant differences in these parameters were found between the two genotypes with occlusal disharmony. In conclusion, occlusal disharmony induces cognitive dysfunction and Aβ accumulation in the rat hippocampus, and the effects of occlusal disharmony on Aβ accumulation and cognitive dysfunction were larger than those of apoE deficiency. © 2013 Ekuni et al.

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  • 還元型コエンザイムQ10の歯周組織の加齢変化に対する効果について

    米田 俊樹, 友藤 孝明, 江國 大輔, 東 哲司, 遠藤 康正, 粕山 健太, 町田 達哉, 森田 学

    Journal of Oral Biosciences Supplement   2013   170 - 170   2013年9月

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  • Anti-aging Effects of Co-enzyme Q10 on Periodontal Tissues

    T. Yoneda, T. Tomofuji, D. Ekuni, T. Azuma, Y. Endo, K. Kasuyama, T. Machida, M. Morita

    JOURNAL OF DENTAL RESEARCH   92 ( 8 )   735 - 739   2013年8月

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    Oxidative stress is associated with age-related reactions. The anti-oxidative effects of a reduced form of co-enzyme Q10 (rCoQ10) suppress oxidative stress, which may contribute to the prevention of age-related inflammatory reactions. We examined the effects of topically applied rCoQ10 on periodontal inflammatory reactions in a rat aging model. Male Fischer 344 rats, 2 (n = 6) and 4 mos (n = 18) of age, were used. All of the two-month-old rats and 6 of the four-month-old rats were sacrificed and 12 remaining four-month-old rats received topically applied ointment with or without 1% rCoQ10 on the gingival surface until they reached 6 mos of age. The rats showed an age-dependent increase in circulating oxidative stress. RCoQ10 decreased oxidative DNA damage and tartrate-resistant acid-phosphatase-positive osteoclasts in the periodontal tissue at 6 mos of age as compared with the control. The same conditions lowered gene expression of caspase-1 and interleukin-1 in the periodontal tissue. Furthermore, Nod-like receptor protein 3 inflammasomes were less activated in periodontal tissues from rCoQ10-treated rats as compared with the control rats. Our results suggest that rCoQ10 suppresses age-related inflammatory reactions and osteoclast differentiation by inhibiting oxidative stress.

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  • Effects of eating behaviors on being overweight in Japanese university students: A cross-sectional survey at the Okayama University 国際誌

    Daisuke Ekuni, Michiko Furuta, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

    Asia-Pacific Journal of Public Health   25 ( 4 )   326 - 334   2013年7月

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    Being overweight is an important risk factor for lifestyle-related diseases. The objective of the present study was to examine whether eating until full, eating quickly, or both eating behaviors were associated with being overweight, defined as a body mass index (BMI) ≥ 25, in young adults. This cross-sectional survey comprised 1918 students (1139 male and 779 female students) at the Okayama University in Japan. In logistic regression analysis, eating until full was not associated with being overweight in either sex. The adjusted odds ratio of being overweight among those who reported eating quickly was 3.93 (2.45-6.31
    P &lt
    .0001) for male and 1.59 (0.79-3.21
    P =.193) for female students. Moreover, the adjusted odds ratio of being overweight was 2.72 (1.72-4.30
    P &lt
    .001) in male students who frequently reported eating a fatty diet. The combination of eating quickly and frequently eating a fatty diet had a supra-additive effect on being overweight in the male students. © 2011 APJPH.

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  • Relationship between masticatory performance and heart rate variability: A pilot study 国際誌

    Noriko Takeuchi, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    ACTA ODONTOLOGICA SCANDINAVICA   71 ( 3-4 )   807 - 812   2013年5月

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    Objective. Little information is available on how impaired masticatory performance relates to heart-rate variability (HRV). The aim of this study was to investigate the relationship between HRV indices and masticatory performance. Materials and methods. Sixty-five subjects (19 men and 46 women, 66.1 +/- 9.9 years old) who had received periodontal maintenance care at the Clinic of Preventive Dentistry of Okayama University Hospital were selected for the study. All subjects completed written questionnaires, a chewing (colour-changing gum) test for masticatory performance and measurement of occlusal force and HRV as well as oral examination. Results. The high sympathetic activity (LF &gt;= 49.6) group showed a significantly lower level of masticatory performance (a(star) value) than the low sympathetic activity group (p &lt; 0.05). There were also significant correlations of masticatory performance with LF and LF/HF (p &lt; 0.05). Conclusion. These findings suggest that there is relationship between masticatory performance and HRV indices and impaired masticatory performance may be a risk factor for inducing high sympathetic activity.

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  • Relationship between periodontal inflammation and fetal growth in pregnant women: a cross-sectional study 国際誌

    Noriko Takeuchi, Daisuke Ekuni, Koichiro Irie, Michiko Furuta, Takaaki Tomofuji, Manabu Morita, Tatsuo Watanabe

    ARCHIVES OF GYNECOLOGY AND OBSTETRICS   287 ( 5 )   951 - 957   2013年5月

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    記述言語:英語   出版者・発行元:SPRINGER HEIDELBERG  

    Periodontal disease in pregnant women, evaluated by probing pocket depth (PPD) or clinical attachment level (CAL), significantly increases the risk of subsequent preterm birth (PB) and/or low birth weight (LBW). However, PPD and CAL do not always reflect current periodontal tissue inflammation. Therefore, assessment of bleeding on probing index (BOP), an indicator of periodontal inflammation, may be appropriate. The objective of this cross-sectional study was to investigate the relationship between periodontal inflammation and fetal growth in pregnant women after periodontal treatment.
    We recruited 203 pregnant women (mean age 31.8 +/- A 4.5 years), including 20 subjects with periodontal inflammation and 183 periodontally healthy controls, after periodontal treatment. Fetal growth parameters as well as periodontal conditions were recorded.
    Perinatal gestational age was 39.4 +/- A 1.3 weeks; 8 subjects had a PB and 12 had an infant with a LBW. Results of multiple stepwise regression analysis indicated that birth length was negatively correlated with BOP% (beta = -0.175; p = 0.002). Fetal femur length, and birth length and weight among mothers with low periodontal inflammation were significantly higher than those among mothers with high periodontal inflammation (p &lt; 0.05). The LBW rate in the high periodontal inflammation group was significantly higher than that in the low periodontal inflammation group (p &lt; 0.05).
    These results suggest that periodontal inflammation is correlated with fetal femur length, birth weight, and birth length.

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  • P-22 岡山大学病院口臭外来における6年間の患者実態調査(ポスター)

    貞森 なみ子, 竹内 倫子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   63 ( 2 )   176 - 176   2013年4月

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    記述言語:日本語   出版者・発行元:有限責任中間法人日本口腔衛生学会  

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  • P-36 アポリポ蛋白E欠損と咬合異常によるラット海馬および認知障害への影響(ポスター)

    江國 大輔, 遠藤 康正, 友藤 孝明, 東 哲司, 入江 浩一郎, 粕山 健太, 米田 俊樹, 森田 学

    口腔衛生学会雑誌   63 ( 2 )   183 - 183   2013年4月

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  • P-56 歯周治療のメインテナンス期における歯周病の進行とコーヒー摂取頻度との関連(ポスター)

    東 哲司, 友藤 孝明, 江國 大輔, 入江 浩一郎, 遠藤 康正, 粕山 健太, 水谷 慎介, 町田 達哉, 森田 学

    口腔衛生学会雑誌   63 ( 2 )   193 - 193   2013年4月

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  • Effects of electrical stimulation on periodontal tissue remodeling in rats

    T. Tomofuji, D. Ekuni, T. Azuma, K. Irie, Y. Endo, K. Kasuyama, M. Nagayama, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   48 ( 2 )   177 - 183   2013年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Background and Objective Electric current is used to promote wound healing. However, it is unclear whether electrical stimulation contributes to gingival tissue remodeling. This study examined the effects of electrical stimulation on gingival tissue remodeling in a rat periodontitis model. Material and Methods Male Wistar rats (n=28, 8wks of age) were divided into four groups of seven rats each. The control group did not receive any treatment for 6wks. In the other groups, periodontitis was ligature-induced for 4wks. After 4wks, the rats with periodontitis were given daily electrical stimulation of 0, 50 or 100A for 2wks. Results The periodontitis group stimulated with 0 A showed a higher density of polymorphonuclear leukocytes and a lower density of collagen in gingival tissue compared with the control group (p&lt;0.05). The two remaining groups treated with 50or 100A of electrical stimulation exhibited a lower density of polymorphonuclear leukocytes (p&lt;0.05) and a higher density of collagen than the group stimulated with 0A (p&lt;0.05). They also showed higher expression of fibroblast growth factor-2 than the group treated with 0A of electrical stimulation (p&lt;0.05). Conclusion Electric stimulation may offer a novel approach to promote gingival tissue remodeling in periodontal lesions.

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  • 大学生における頬粘膜圧痕の形成にかかわる因子

    水谷 慎介, 江國 大輔, 友藤 孝明, 入江 浩一郎, 東 哲司, 森田 学

    口腔衛生学会雑誌   63 ( 3 )   291 - 291   2013年4月

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  • Lipopolysaccharide induces a stromal-epithelial signalling axis in a rat model of chronic periodontitis 国際誌

    James D. Firth, Daisuke Ekuni, Koichiro Irie, Takaaki Tomofuji, Manabu Morita, Edward E. Putnins

    JOURNAL OF CLINICAL PERIODONTOLOGY   40 ( 1 )   8 - 17   2013年1月

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    Aim Lipopolysaccharide is a bacterial virulence factor implicated in chronic periodontitis, which may penetrate the junctional epithelial barrier and basement membrane to insult underlying stroma. We sought to identify lipopolysaccharide-induced global gene expression changes responsible for signalling between stroma and epithelium during disease onset.
    Materials and Methods Using a rat lipopolysaccharide periodontitis model, junctional epithelium and underlying stromal tissue were separately collected from healthy and diseased animals by laser-capture microdissection and subject to gene expression microarray analysis. Key gene products identified were validated in gingival epithelial and fibroblast cell cultures.
    Results Global gene expression patterns distinguishing health versus disease were found in and between both tissue types. In stroma, the most significantly altered gene ontology function group (Z &gt;= 4.00) was cytokines, containing most significantly (+/- 2-fold; p &lt; 0.05) upregulated genes amphiregulin, IL1-beta and Fas ligand, all positive, diffusible modulators of the epithelial growth factor receptor pathway. In epithelium, the most significant changes were in downregulated FOS-related antigen-1 gene, somatostatin receptor-2 gene and mucin-4 gene, all negative modulators of the epithelial growth factor receptor pathway.
    Conclusion These results establish a periodontitis model for studying gene product interactions and suggests that the onset of junctional epithelial disease hyperproliferation involves a concerted stromalepithelial signalling axis.

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  • Preventive effects of trehalose on osteoclast differentiation in rat periodontitis model 国際誌

    Yasumasa Endo, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Kenta Kasuyama, Manabu Morita

    JOURNAL OF CLINICAL PERIODONTOLOGY   40 ( 1 )   33 - 40   2013年1月

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    Aim Trehalose, which is a disaccharide formed by a 1,1 linkage of two glucose molecules, was suggested to have a suppressive effect on bone resorption. In this study, we examined the effects of topical application of trehalose on osteoclast differentiation in a rat periodontitis model.
    Material and Methods Rats were divided into four groups. One group received no treatment. In the other groups, experimental periodontitis was induced by ligature placement. These rats with experimental periodontitis received topical application of pure water (vehicle group), 30 mg/ml trehalose solution (30 mg/ml trehalose group) or 60 mg/ml trehalose solution (60 mg/ml trehalose group) to the gingival sulcus respectively.
    Results The vehicle group showed higher numbers of polymorphonuclear leucocytes, receptor activator of nuclear factor kappa B ligand (RANKL)-positive cells and osteoclasts compared with the no treatment group respectively. Trehalose-applied groups exhibited lower numbers of these cells compared with the vehicle group. Gene expressions of tumour necrosis factor-a, RANKL and toll-like receptor 4 were suppressed by trehalose. In addition, protein expressions of RANKL inducing pathway were less activated by trehalose.
    Conclusion Topical application of trehalose could suppress osteoclast differentiation by inactivation of RANKL inducing pathway in the rat periodontitis model.

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  • Involvement of toll-like receptor 2 and 4 in association between dyslipidemia and osteoclast differentiation in apolipoprotein E deficient rat periodontium 国際誌

    Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Kenta Kasuyama, Toshiki Yoneda, Manabu Morita

    LIPIDS IN HEALTH AND DISEASE   12 ( 1 )   1 - 1   2013年1月

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    Background: Dyslipidemia increases circulating levels of oxidized low-density lipoprotein (OxLDL) and this may induce alveolar bone loss through toll-like receptor (TLR) 2 and 4. The purpose of this study was to investigate the effects of dyslipidemia on osteoclast differentiation associated with TLR2 and TLR4 in periodontal tissues using a rat dyslipidemia (apolipoprotein E deficient) model.
    Methods: Levels of plasma OxLDL, and the cholesterol and phospholipid profiles in plasma lipoproteins were compared between apolipoprotein E-deficient rats (16-week-old males) and wild-type (control) rats. In the periodontal tissue, we evaluated the changes in TLR2, TLR4, receptor activator of nuclear factor kappa B ligand (RANKL) and tartrate resistant acid phosphatase (TRAP) expression.
    Results: Apolipoprotein E-deficient rats showed higher plasma levels of OxLDL than control rats (p&lt;0.05), with higher plasma levels of total cholesterol (p&lt;0.05) and LDL-cholesterol (p&lt;0.05) and lower plasma levels of high-density lipoprotein cholesterol (p&lt;0.05). Their periodontal tissue also exhibited a higher ratio of RANKL-positive cells and a higher number of TRAP-positive osteoclasts than control rats (p&lt;0.05). Furthermore, periodontal gene expression of TLR2, TLR4 and RANKL was higher in apolipoprotein E-deficient rats than in control rats (p&lt;0.05).
    Conclusion: These findings underscore the important role for TLR2 and TLR4 in mediating the osteoclast differentiation on alveolar bone response to dyslipidemia.

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  • 頭頸部がん患者における予後推定栄養指数と口腔内状態との関係

    丸山貴之, 友藤孝明, 水谷慎介, 江國大輔, 森田 学

    口腔衛生学会雑誌   63 ( 4 )   321 - 327   2013年

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    頭頸部がん治療の予後を知るうえで,小野寺の予後推定栄養指数(O-PNI)は重要な指標であるが,O-PNIと栄養摂取器官である口腔内の状態との関連について検討したものはない.本研究の目的は,頭頸部がん患者におけるO-PNIと口腔内状態との関連を調べることである.嚥下機能への影響を考慮するため,がんの発生部位を口腔内と口腔外に分け,それぞれのO-PNIと口腔内状態との関連について比較検討した.岡山大学病院にて頭頸部がんと診断を受けたがん治療開始前の150名を分析対象とした.がんの部位,進行度(ステージ),body mass index,飲酒経験,喫煙量,肝機能,腎機能,C反応性タンパク(CRP),口腔内指標を調べた.口腔内外それぞれのがんにおいて,O-PNIと各指標との重回帰分析を行い,O-PNIとの関連因子について検討した.重回帰分析の結果,口腔がん患者においてはO-PNIと年齢(p=0.017),平均クリニカルアタッチメントレベル(p=0.049),CRP(p<0.001)との間に関連を認めた.一方,口腔がん以外の頭頸部がん患者においてはO-PNIとCRP(p=0.005)との間に関連を認めたが,口腔内指標との関連は認められなかった.以上より,頭頸部がん患者におけるO-PNIを左右する因子として,口腔内のがん患者については歯周状態が関連しているのに対し,口腔外のがん患者については口腔内指標との関連はなかった.

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  • Self-reported aural symptoms, headache and temporomandibular disorders in Japanese young adults

    Rahena Akhter, Manabu Morita, Daisuke Ekuni, Nur Mohammad Monsur Hassan, Michiko Furuta, Reiko Yamanaka, Yoshizo Matsuka, David Wilson

    BMC Musculoskeletal Disorders   14   2013年

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    Background: To investigate the associations of aural symptoms, headache and depression with the presence of temporomandibular disorder (TMD) symptoms in a young adult population in Japan. Methods. A personal interview survey was conducted on first-year university students (n = 1,930) regarding symptoms of TMD, aural problems, headache, shoulder pain and depression. Logistic regression was applied to assess the associations of these problems with the presence of TMD symptoms after controlling for age and gender. Results: Among the 1,930 students, 543 students exhibited TMD symptoms and were classified into 7 groups: clicking only (Group I, n = 319), pain in the TMJ only (Group II, n = 21), difficulty in mouth opening only (Group III, n = 18), clicking and pain (Group IV, n = 29), clicking and difficulty in mouth opening (Group V, n = 48), difficulty in mouth opening and pain (Group VI, n = 11), and combination of three symptoms (Group VII, n = 97). The control group (n = 1,387) were subjects without any TMD symptoms. After adjusting for age and gender, a strong association was observed between TMD symptoms (Group II and IV) and tinnitus (OR = 12.1 and 13.2, respectively). TMD symptoms (Group I, II and III) were also associated with vertigo and headache. Otalgia and depression were significantly associated with the presence of clicking only. Conclusions: TMD symptoms were significantly correlated to aural symptoms and headache. A functional evaluation of the stomatognathic system should be considered in subjects with unexplained aural symptoms and headache. © 2013 Akhter et al.
    licensee BioMed Central Ltd.

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  • Dental caries is correlated with knowledge of comprehensive food education in Japanese university students 国際誌

    Daisuke Ekuni, Takaaki Tomofuji, Shinsuke Mizutani, Michiko Furuta, Koichiro Irie, Tetsuji Azuma, Azusa Kojima, Yoshiaki Iwasaki, Manabu Morita

    ASIA PACIFIC JOURNAL OF CLINICAL NUTRITION   22 ( 2 )   312 - 318   2013年

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    In Japan, the "Food Education Basic Law" (The Basic Law on Shokuiku, food education) was enacted in 2005. The comprehensive food education programs, namely Shokuiku, aim to improve dietary practices to reduce lifestyle-related diseases. Dental caries is one of the diseases associated with inappropriate dietary habits. Thus, food education may influence the prevalence of dental caries. However, there are no data regarding the association between public based-food education and dental caries. The aim of the present study was to investigate the relationship between knowledge about comprehensive food education (Shokuika) and dental caries experience in Japanese university students. A total of 2,184 students (1,240 men, 944 women), aged 18-20 years, were examined. They had attended the Shokuiku program while in junior/senior high school. The numbers of teeth present, and decayed, missing and filled teeth (DMFT) (dental caries experience) were recorded. Additional information was collected via a questionnaire regarding knowledge about food education, dietary habits and oral health behavior. Of the students, 315 men (20.7%) and 345 women (52.8%) reported that they know and can explain the meaning and content of the word "Shokuiku". After adjusting for potential confounding factors, subjects who did not have knowledge about Shokuiku had higher adjusted odds ratio (OR) for dental caries experience (DMFT &gt;0) than those who had (adjusted OR, 1.23; 95% CI, 1.02-1.48; p&lt;0.05). These observations revealed that having knowledge about comprehensive food education in university students correlates with low prevalence of dental caries.

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  • Hydrogen-rich water prevents lipid deposition in the descending aorta in a rat periodontitis model 国際誌

    Daisuke Ekuni, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Shinsuke Mizutani, Azusa Kojima, Manabu Morita

    ARCHIVES OF ORAL BIOLOGY   57 ( 12 )   1615 - 1622   2012年12月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: Periodontitis has been causally linked to atherosclerosis, which is mediated by the oxidative stress. As hydrogen-rich water (HW) scavenges reactive oxygen species (ROS), we hypothesized that HW could prevent lipid deposition induced by periodontitis in the aorta. The aim of this study was to investigate the effects of HW on the initiation of atherosclerosis in a rat periodontitis model.
    Design: Eighteen 8-wk-old male Wistar rats were divided into three groups of six rats; the periodontitis group, periodontitis + HW group and the no treatment (control) group. In the periodontitis and periodontitis + HW groups, periodontitis was induced using a ligature for 4 wk, while the periodontitis + HW group was given water containing 800-1000 mu g/L hydrogen during the 4-wk experimental period.
    Results: In the periodontitis group, lipid deposition in the descending aorta was observed. The periodontitis group also showed significant higher serum levels for ROS and oxidised low-density lipoprotein-cholesterol (ox-LDL) (1.7 and 1.4 times, respectively), and higher aortic expression levels of nitrotyrosine and hexanoyl-lysine (HEL) (7.9 and 16.0 times, respectively), as compared to the control group (p &lt; 0.05). In the periodontitis + HW group, lipid deposition was lower. Lower serum levels of ROS and ox-LDL (0.46 and 0.82 times, respectively) and lower aortic levels of nitrotyrosine and HEL (0.27 and 0.19 times, respectively) were observed in the periodontitis + HW group than in the periodontitis group (p &lt; 0.05).
    Conclusions: HW intake may prevent lipid deposition in the rat aorta induced by periodontitis by decreasing serum ox-LDL levels and aortic oxidative stress. (C) 2012 Elsevier Ltd. All rights reserved.

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  • Gum chewing modulates heart rate variability under noise stress 国際誌

    Daisuke Ekuni, Takaaki Tomofuji, Noriko Takeuchi, Manabu Morita

    ACTA ODONTOLOGICA SCANDINAVICA   70 ( 6 )   491 - 496   2012年12月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    Objective. Gum chewing may relieve stress, although this hypothesis has not been proven. Heart-rate variability (HRV) is commonly used to measure stress levels. However, it is not known if gum chewing modulates HRV under acute stress. The aim of this study was to investigate the effects of gum chewing on HRV under acute stress. Materials and methods: A crossover study involving 47 non-smoking healthy subjects, aged 22-27 years, was carried out. The subjects received a stress procedure with gum chewing (GS group) and without gum chewing (S group). Additionally, the other 20 subjects were allocated to the gum chewing without stress group (G group). The GS and S groups were exposed to noise for 5 min (75 dBA) as stress. Before and after stress exposure/gum chewing, participants completed the state portion of the State-Trait Anxiety Inventory (STAI-s) and a single Stress Visual Analogue Scale (VAS) measurement. HRV measurement was performed before and during stress/gum chewing for 5 min. Results. After the stress procedure, VAS score significantly increased in the GS and S groups. During the stress procedure, the GS group showed a significantly lower level of high frequency (HF) and higher levels of low frequency (LF) and LF/HF than the S group. However, there were no significant differences in the scores of the state portion of the State-Trait Anxiety Inventory (STAI-s) and VAS between the two stress groups. Conclusions. These findings suggest that gum chewing modulates HRV, but may not relieve acute stress caused by noise.

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  • 岡山大学新入生の自覚的口腔健康観に影響する因子の検討

    児島 梓, 江國 大輔, 友藤 孝明, 東 哲司, 入江 浩一郎, 水谷 慎介, 森田 学

    岡山歯学会雑誌   31 ( 2 )   97 - 97   2012年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 日本とカンボジアの若年者におけるソーシャル・キャピタルと口腔の健康の関係

    古田 美智子, 野中 愛恵, 江國 大輔, 森田 学, 高尾 総司, 山下 喜久

    日本公衆衛生学会総会抄録集   71回   583 - 583   2012年10月

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  • Relationship Between Serum Albumin Concentration and Periodontal Condition in Patients With Head and Neck Cancer 国際誌

    Takayuki Maruyama, Reiko Yamanaka, Aya Yokoi, Daisuke Ekuni, Takaaki Tomofuji, Nobuyoshi Mizukawa, Tomoo Onoda, Motoharu Eguchi, Manabu Morita

    JOURNAL OF PERIODONTOLOGY   83 ( 9 )   1110 - 1115   2012年9月

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    記述言語:英語   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Serum albumin concentration is known to be an independent predictor of survival in head and neck cancer. The previous studies suggested relationships between serum albumin concentration and oral health status in populations without serious systemic disorders. However, these relationships remain unclear in patients with head and neck cancer. The purpose of this study is to investigate the relationship between serum albumin concentration and oral health status in patients with head and neck cancer.
    Methods: Fifty individuals diagnosed with primary head and neck cancer, 25 individuals with normal serum albumin concentration (&gt;= 3.85 g/dL), and 25 age- and sex-matched individuals with lower serum albumin concentration (&lt;3.85 g/dL) were analyzed. General status, including cancer stage, body mass index, drinking and smoking habits, and biochemical serum markers, were evaluated. Oral health status, including periodontal condition and occlusion tooth pairs, were also evaluated.
    Results: Mean clinical attachment level (CAL) and C-reactive protein in the lower serum albumin concentration group were greater than those in the normal serum albumin concentration group (P = 0.009 and P = 0.002, respectively). However, there were no significant differences in any other oral and/or serum parameters between the two serum albumin groups. A logistic regression model showed that mean CAL was significantly associated with high or low levels of serum albumin concentration (odds ratio = 9.752; 95% confidence interval = 1.702 to 55.861; P = 0.011).
    Conclusions: This study suggests an association between periodontal disease and serum albumin concentration in patients with head and neck cancer. Longitudinal studies are necessary to examine the causal relationship between serum albumin concentration and periodontal condition. J Periodontol 2012;83:1110-1115.

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  • Effects of self-efficacy on oral health behaviours and gingival health in university students aged 18- or 19-years-old 国際誌

    Shinsuke Mizutani, Daisuke Ekuni, Michiko Furuta, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Azusa Kojima, Jun Nagase, Yoshiaki Iwasaki, Manabu Morita

    JOURNAL OF CLINICAL PERIODONTOLOGY   39 ( 9 )   844 - 849   2012年9月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Aim Although self-efficacy is known to affect various health-related practises, few studies have clearly examined how self-efficacy correlates with oral health behaviors or the oral health condition. We examined the relationship between gingivitis, oral health behaviors and self-efficacy in university students. Material & Methods A total of 2,111 students (1,197 males, 914 females) aged 18 and 19 years were examined. The degree of gingivitis was expressed as the percentage of bleeding on probing (%BOP). Additional information was collected via a questionnaire regarding oral health behaviors (daily frequency of tooth-brushing, use of dental floss and regular check-up). Self-efficacy was assessed using the Self-Efficacy Scale for Self-care (SESS). Path analysis was used to test pathways from self-efficacy to oral health behaviors and %BOP. Results In the final structural model, self-efficacies were related to each other, and they affected oral health behaviors. Good oral health behaviors reduced dental plaque and calculus, and lower levels of dental plaque and calculus resulted in lower %BOP. Conclusion Higher self-efficacy correlated with better oral health behaviours and gingival health in university students. Improving self-efficacy may be beneficial for maintaining good gingival health in university students. To prevent gingivitis, the approach of enhancing self-efficacy in university students would be useful.

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  • Self-reports of eating quickly are related to a decreased number of chews until first swallow, total number of chews, and total duration of chewing in young people 国際誌

    Daisuke Ekuni, Michiko Furuta, Noriko Takeuchi, Takaaki Tomofuji, Manabu Morita

    ARCHIVES OF ORAL BIOLOGY   57 ( 7 )   981 - 986   2012年7月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: The validity of a self-questionnaire about eating quickly remains unclear. If a significant relationship between subjective and objective methods to evaluate eating quickly can be confirmed, then the subjective method can be widely and reliably used in many fields. This study investigated relationships between subjective and objective methods to evaluate eating quickly and also numerically characterized the kinesis of eating quickly in young people.
    Design: One hundred and thirteen students (44 males and 69 females; mean age 22.8 +/- 2.0 years) were selected. All subjects completed written questionnaires, and number of chews until first swallow, total duration of chewing, number of chews, chewing rate and bite size were measured using test products (a Japanese cracker and rice ball).
    Results: Both male and female subjects who reported eating quickly showed a significantly lower number of chews until first swallow (Japanese cracker), a lower number of chews overall (rice ball), and a shorter total duration of chewing (rice ball) than other subjects. There was no difference in chewing rate between subjects who ate quickly or not.
    Conclusions: These findings suggest that using test products, self-reports of eating quickly are related to a decreased number of chews until first swallow, total number of chews, and total duration of chewing, but not chewing rate, and that a self-reported questionnaire to evaluate eating rate is valid in young people. (C) 2012 Elsevier Ltd. All rights reserved.

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  • 小学生における永久歯の萠出状況の報告

    横井 彩, 山中 玲子, 江國 大輔, 丸山 貴之, 入江 浩一郎, 遠藤 康正, 水谷 慎介, 友藤 孝明, 森田 学

    岡山歯学会雑誌   31 ( 1 )   39 - 40   2012年6月

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  • 耳鼻咽喉科病棟看護師を対象に口腔ケアに関するアンケートの実施とその報告

    志茂 加代子, 岡崎 惠子, 三浦 留美, 山中 玲子, 水川 展吉, 江国 大輔, 丸山 貴之, 横井 彩, 仲田 直樹, 武田 斉子, 喜田 沙音里, 森田 学

    岡山歯学会雑誌   31 ( 1 )   34 - 35   2012年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • ラット歯肉コラーゲンの加齢変化に対するトマリナの効果

    友藤 孝明, 入江 浩一郎, 江國 大輔, 遠藤 康正, 粕山 健太, 東 哲司, 玉木 直文, 森田 学

    日本抗加齢医学会総会プログラム・抄録集   12回   209 - 209   2012年6月

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    記述言語:日本語   出版者・発行元:(一社)日本抗加齢医学会  

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  • Loss of claudin-1 in lipopolysaccharide-treated periodontal epithelium

    T. Fujita, J. D. Firth, M. Kittaka, D. Ekuni, H. Kurihara, E. E. Putnins

    JOURNAL OF PERIODONTAL RESEARCH   47 ( 2 )   222 - 227   2012年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Background and Objective: The epithelial barrier is a critical component of innate immunity and provides protection against microbial invasion. Claudin-1, a tight junction protein, is known to contribute to the epithelial cell barrier. An experimentally induced rat periodontal disease model was used to study the effects of lipopolysaccharide (LPS) on the expression of tight junction-associated molecule genes in the junctional epithelium.
    Material and Methods: LPS was applied for 8 wk in the gingival sulcus, and junctional epithelium was collected by laser-capture microdissection and subjected to microarray analysis.
    Results: Microarray analysis identified that expression of the claudin-1 gene was decreased in the epithelium by chronic LPS challenge. Immunohistochemical analysis confirmed the expression of claudin-1 protein in junctional epithelium and that 8 wk of chronic LPS topical application significantly reduced claudin-1 expression. The effect of LPS on claudin-1 protein expression was validated using a porcine junctional epithelial cell culture Transwell model. The epithelial barrier, as measured using transmembrane resistance, was significantly reduced after 3 wk of LPS challenge and this was associated with a decreased level of expression of claudin-1 protein.
    Conclusion: These results confirm that the initiation of experimental periodontal disease is associated with reduction in the expression of claudin-1 gene and protein. This decreased level of a critical tight junction protein may result in the disruption of barrier function and may play an important role in the initiation of periodontal disease.

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  • Effects of topical application of inorganic polyphosphate on tissue remodeling in rat inflamed gingiva

    K. Kasuyama, T. Tomofuji, D. Ekuni, T. Azuma, K. Irie, Y. Endo, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   47 ( 2 )   159 - 164   2012年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Background and Objective: Inorganic polyphosphate [poly(P)] is a biopolymer found in almost all cells and tissues, and which promotes tissue remodeling. However, there is limited information on how poly(P) affects the connective tissue in inflamed gingiva. This study examined the effects of topical application of poly(P) on gingival connective tissue and its remodeling in a rat periodontitis model.
    Material and Methods: Male Wistar rats (n = 36, 8 wk of age) were used in this 6-wk study. The rats were divided into six groups of six rats each. The control group received no treatment. In the other groups, periodontitis was ligatureinduced for 4 wk. After 4 wk, the rats with periodontitis were further divided into five groups, and were left untreated (periodontitis group) or subjected to topical application of oral rinses containing 0, 0.1, 1 or 5% poly(P) for 2 wk.
    Results: The periodontitis and 0% poly(P) groups showed a higher density of polymorphonuclear leukocytes and a lower density of collagen in gingival tissue than the control group (p &lt; 0.05). In contrast, groups treated with more than 1% poly(P) exhibited a lower density of polymorphonuclear leukocytes (p &lt; 0.05) and a higher density of collagen than the periodontitis and 0% poly(P) groups (p &lt; 0.05). A higher expression of fibroblast growth factor-2 was observed in the gingiva of rats treated with 1% poly(P) than in those treated with 0% poly(P) (p &lt; 0.05).
    Conclusion: Topical application of poly(P) may induce connective tissue remodeling, contributing to improvement of inflamed gingiva in rats.

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  • Effects of topical application of inorganic polyphosphate on tissue remodeling in rat inflamed gingiva

    K. Kasuyama, T. Tomofuji, D. Ekuni, T. Azuma, K. Irie, Y. Endo, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   47 ( 2 )   159 - 164   2012年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Background and Objective: Inorganic polyphosphate [poly(P)] is a biopolymer found in almost all cells and tissues, and which promotes tissue remodeling. However, there is limited information on how poly(P) affects the connective tissue in inflamed gingiva. This study examined the effects of topical application of poly(P) on gingival connective tissue and its remodeling in a rat periodontitis model.
    Material and Methods: Male Wistar rats (n = 36, 8 wk of age) were used in this 6-wk study. The rats were divided into six groups of six rats each. The control group received no treatment. In the other groups, periodontitis was ligatureinduced for 4 wk. After 4 wk, the rats with periodontitis were further divided into five groups, and were left untreated (periodontitis group) or subjected to topical application of oral rinses containing 0, 0.1, 1 or 5% poly(P) for 2 wk.
    Results: The periodontitis and 0% poly(P) groups showed a higher density of polymorphonuclear leukocytes and a lower density of collagen in gingival tissue than the control group (p &lt; 0.05). In contrast, groups treated with more than 1% poly(P) exhibited a lower density of polymorphonuclear leukocytes (p &lt; 0.05) and a higher density of collagen than the periodontitis and 0% poly(P) groups (p &lt; 0.05). A higher expression of fibroblast growth factor-2 was observed in the gingiva of rats treated with 1% poly(P) than in those treated with 0% poly(P) (p &lt; 0.05).
    Conclusion: Topical application of poly(P) may induce connective tissue remodeling, contributing to improvement of inflamed gingiva in rats.

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  • Social capital and self-rated oral health among young people 国際誌

    Michiko Furuta, Daisuke Ekuni, Soshi Takao, Etsuji Suzuki, Manabu Morita, Ichiro Kawachi

    COMMUNITY DENTISTRY AND ORAL EPIDEMIOLOGY   40 ( 2 )   97 - 104   2012年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Objectives: A few studies have revealed the impact of neighborhood social capital on oral health among young people. We sought to examine the associations of social capital in three settings (families, neighborhoods, and schools) with self-rated oral health among a sample of college students in Japan. Methods: Cross-sectional survey of 967 students in Okayama University, aged 18 and 19 years, was carried out. Logistic regression was used to examine the associations of poor self-rated oral health with perceptions of social capital, adjusting for self-perceived household income category and oral health behaviors. Results: The prevalence of subjects with poor self-rated oral health was 22%. Adjusted for gender, self-perceived household income category, dental fear, toothbrush frequency, and dental floss use, poor self-rated oral health was significantly associated with lower level of neighborhood trust [odds ratio (OR) 2.22; 95% confidence interval (CI): 1.40-3.54] and lower level of vertical trust in school (OR 1.71; 95% CI: 1.05-2.80). Low informal social control was unexpectedly associated with better oral health (OR 0.54; 95% CI: 0.34-0.85). Conclusion: The association of social capital with self-rated oral health is not uniform. Higher trust is associated with better oral health, whereas higher informal control in the community is associated with worse oral health.

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  • Social capital and self-rated oral health among young people 国際誌

    Michiko Furuta, Daisuke Ekuni, Soshi Takao, Etsuji Suzuki, Manabu Morita, Ichiro Kawachi

    COMMUNITY DENTISTRY AND ORAL EPIDEMIOLOGY   40 ( 2 )   97 - 104   2012年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Objectives: A few studies have revealed the impact of neighborhood social capital on oral health among young people. We sought to examine the associations of social capital in three settings (families, neighborhoods, and schools) with self-rated oral health among a sample of college students in Japan. Methods: Cross-sectional survey of 967 students in Okayama University, aged 18 and 19 years, was carried out. Logistic regression was used to examine the associations of poor self-rated oral health with perceptions of social capital, adjusting for self-perceived household income category and oral health behaviors. Results: The prevalence of subjects with poor self-rated oral health was 22%. Adjusted for gender, self-perceived household income category, dental fear, toothbrush frequency, and dental floss use, poor self-rated oral health was significantly associated with lower level of neighborhood trust [odds ratio (OR) 2.22; 95% confidence interval (CI): 1.40-3.54] and lower level of vertical trust in school (OR 1.71; 95% CI: 1.05-2.80). Low informal social control was unexpectedly associated with better oral health (OR 0.54; 95% CI: 0.34-0.85). Conclusion: The association of social capital with self-rated oral health is not uniform. Higher trust is associated with better oral health, whereas higher informal control in the community is associated with worse oral health.

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  • Supplementation of broccoli or Bifidobacterium longum-fermented broccoli suppresses serum lipid peroxidation and osteoclast differentiation on alveolar bone surface in rats fed a high-cholesterol diet 国際誌

    Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Tatsuo Yamamoto, Atsushi Ishikado, Takehiko Sato, Kayo Harada, Hirohisa Suido, Manabu Morita

    NUTRITION RESEARCH   32 ( 4 )   301 - 307   2012年4月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    High-cholesterol diet enhances osteoclastic activity on alveolar bone by increasing serum lipid peroxidation. We hypothesized that supplementation with dietary antioxidants, such as found in broccoli and its fermented products, might suppress increases in serum lipid peroxidation, contributing to the inhibition of osteoclastic activity after high-cholesterol diet intake. The purpose of the present study was to investigate the effects of broccoli and fermented broccoli consumption on serum lipid peroxidation and osteoclast differentiation in alveolar bone of rats fed a high-cholesterol diet. In this 12-week study, rats were divided into 4 groups (n = 6/group): a control group (fed regular diet) and 3 experimental groups (fed a high-cholesterol [1% wt/wt] diet, or a high-cholesterol diet supplemented with either broccoli powder [5% wt/wt] or Bifidobacterium longum-fermented broccoli powder [5% wt/wt]). Serum hexanoyl-lysine (HEL) levels were measured as a parameter of lipid peroxidation. The number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts in alveolar bone was enumerated to evaluate osteoclast differentiation. When compared with regular diet, the high-cholesterol diet increased serum HEL levels and resulted in a higher number of TRAP-positive osteoclasts at 12 weeks. The high-cholesterol diet supplemented with broccoli or B. longum-fermented broccoli showed lower levels of serum HEL and fewer TRAP-positive osteoclasts than the high-cholesterol diet at 12 weeks. In conclusion, consumption of broccoli, or its fermented product, inhibited the effects of a high-cholesterol diet on osteoclast differentiation in rat alveolar bone by suppressing serum lipid peroxidation. (C) 2012 Elsevier Inc. All rights reserved.

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  • Combined Effects of Hydrogen Sulfide and Lipopolysaccharide on Osteoclast Differentiation in Rats 国際誌

    Koichiro Irie, Daisuke Ekuni, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Ken Yaegaki, Manabu Morita

    JOURNAL OF PERIODONTOLOGY   83 ( 4 )   522 - 527   2012年4月

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    記述言語:英語   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Lipopolysaccharide (LPS) stimulates osteoclast differentiation through toll-like receptors (TLRs) 2 and 4, and hydrogen sulfide (H2S) induces osteoclast differentiation. If H2S activates TLRs, H2S may enhance the effects of LPS on osteoclast differentiation. The purpose of the present study is to examine the combined effects of sodium hydrogen sulfide (NaHS, an H2S donor drug) and LPS on osteoclast differentiation and TLR expression in rat periodontal tissue.
    Methods: Twenty-eight male Wistar rats (8 weeks old) were divided into four groups (n = 7 per group): a control (no treatment) group and three experimental groups (NaHS group, LPS group, and a combination [NaHS + LPS] group). At 1 day after topical application of NaHS and/or Porphyromonas gingivalis LPS into the gingival sulcus of first molars, the number of tartrate-resistant acid phosphate (TRAP)-positive osteoclasts in the periodontal tissue was counted. Expression of TLR2 and TLR4 mRNAs and proteins in the gingival was also assessed.
    Results: The number of TRAP-positive osteoclasts was significantly higher in the combination group than in any other group (P &lt; 0.01). The combination group had 11.0-fold higher TLR4 mRNA levels than the control group. TLR4 protein levels were also higher in the combination group than in the NaHS or LPS group. However, the TLR2 mRNA and protein levels were not significantly different in the combination group and the LPS group.
    Conclusion: In rat periodontal tissue, NaHS and LPS had an additive effect on osteoclast differentiation through activation of the TLR4 pathway but not the TLR2 pathway. J Periodontol 2012;83:522-527.

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  • Loss of claudin-1 in lipopolysaccharide-treated periodontal epithelium

    T. Fujita, J. D. Firth, M. Kittaka, D. Ekuni, H. Kurihara, E. E. Putnins

    JOURNAL OF PERIODONTAL RESEARCH   47 ( 2 )   222 - 227   2012年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Background and Objective: The epithelial barrier is a critical component of innate immunity and provides protection against microbial invasion. Claudin-1, a tight junction protein, is known to contribute to the epithelial cell barrier. An experimentally induced rat periodontal disease model was used to study the effects of lipopolysaccharide (LPS) on the expression of tight junction-associated molecule genes in the junctional epithelium.
    Material and Methods: LPS was applied for 8 wk in the gingival sulcus, and junctional epithelium was collected by laser-capture microdissection and subjected to microarray analysis.
    Results: Microarray analysis identified that expression of the claudin-1 gene was decreased in the epithelium by chronic LPS challenge. Immunohistochemical analysis confirmed the expression of claudin-1 protein in junctional epithelium and that 8 wk of chronic LPS topical application significantly reduced claudin-1 expression. The effect of LPS on claudin-1 protein expression was validated using a porcine junctional epithelial cell culture Transwell model. The epithelial barrier, as measured using transmembrane resistance, was significantly reduced after 3 wk of LPS challenge and this was associated with a decreased level of expression of claudin-1 protein.
    Conclusion: These results confirm that the initiation of experimental periodontal disease is associated with reduction in the expression of claudin-1 gene and protein. This decreased level of a critical tight junction protein may result in the disruption of barrier function and may play an important role in the initiation of periodontal disease.

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  • コーヒー摂取と歯周病の関連

    東 哲司, 友藤 孝明, 江國 大輔, 入江 浩一郎, 遠藤 康正, 粕山 健太, 森田 学

    口腔衛生学会雑誌   62 ( 2 )   224 - 224   2012年4月

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  • 歯肉コラーゲンの加齢変化に対するピクノジェノール配合歯磨剤の効果

    粕山 健太, 友藤 孝明, 江國 大輔, 東 哲司, 入江 浩一郎, 遠藤 康正, 森田 学

    日本歯周病学会会誌   54 ( 春季特別 )   132 - 132   2012年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周病メインテナンス期患者における歯周病の進行と全身の酸化ストレスとの関連

    玉木 直文, 友藤 孝明, 江國 大輔, 森田 学

    日本歯周病学会会誌   54 ( 春季特別 )   135 - 135   2012年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 動物モデルを用いた基礎研究からヘルスプロモーションへ 歯周病の研究を行ってきた一人として

    江國 大輔

    口腔衛生学会雑誌   62 ( 2 )   144 - 145   2012年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 咬合異常はラット海馬におけるβアミロイド量を増加させる

    江國 大輔, 友藤 孝明, 入江 浩一郎, 東 哲司, 遠藤 康正, 粕山 健太, 森田 学

    口腔衛生学会雑誌   62 ( 3 )   340 - 340   2012年4月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 大学新入生の口腔健康状態の調査(性差および経年的変化の検討).

    児島 梓, 江國大輔, 古田美智子, 入江浩一郎, 東 哲司, 水谷慎介, 山中玲子, 友藤孝明, 森田 学, 山本龍生, 村上 隆, 山城 隆, 古賀 光, 岩崎良章, 清水幸登, 大西 勝, 小倉俊郎

    CAMPUS HEALTH   49 ( 3 )   91 - 96   2012年

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    記述言語:日本語   出版者・発行元:(公社)全国大学保健管理協会  

    岡山大学の新入生を対象とした歯科健診の結果について、性差および経年的変化の検討を行った概要を報告する。2008年度から2011年度の新入生のうち18〜19歳の者を抽出し、総計5,097名(男性2,913名、女性2,184名)を対象に口腔健康状態を分析した。う蝕の保有者は全体の7.4%〜16.1%で、性差および経年的な傾向はなかった。一方、4mm以上の歯周ポケットを有する者の割合はCPIでは11.0%〜22.6%であり、経年的に軽症化にあった。男女比較では、プロービング時出血の割合、歯垢指数、歯石指数のすべてにおいて女性が有意に少なかった。さらに、智歯が1本も萠出していない者の割合は68.6%〜72.1%で、有意に女性が多かったが、年度間の差はなかった。不正咬合の割合は27.5%〜38.9%で経年的に減少し、歯科矯正治療経験者の割合は9.1%〜12.2%で経年的に増加していた。また、男女比較では両割合ともに女性が有意に多かった。以上より、大学新入生において、女性は男性に比べて歯周状態および口腔衛生状態が優れている一方で、不正咬合を有する者が多かった。また、経年的にはう蝕の保有者の割合には一定の傾向はなかったが、歯周状態と不正咬合は改善傾向にあった。今後も大学生の口腔の健康を保持・増進するために、歯科健診や保健指導を行う必要がある。(著者抄録)

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  • 岡山大学新入生におけるう蝕経験の有無と食生活に対する自己効力感との関係.

    水谷慎介, 江國大輔, 古田美智子, 友藤孝明, 入江浩一郎, 東 哲司, 児島 梓, 長瀬 純, 森田 学

    口腔衛生学会雑誌   62 ( 4 )   403 - 409   2012年

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    記述言語:日本語   出版者・発行元:有限責任中間法人日本口腔衛生学会  

    これまでう蝕のリスク因子について多くの研究がなされてきたが,食生活に対する自己効力感が関係するかどうかは不明である.本研究の目的は,大学生新入生を対象に食生活に対する自己効力感とう蝕経験歯(DMF歯)の有無との関連を検討することである.対象者は2011年度岡山大学新入生のうち18,19歳の2,031名(男性1,159名,女性872名)である.アンケートにより,性別,食生活,フッ化物歯面塗布経験の有無,フッ化物配合歯磨剤の使用の有無および食生活に対する自己効力感に関する質問を行い,口腔内診査でDMF歯数を調べた.DMF歯の有無によって対象者を2群に分け,各項目について比較した.さらに,ロジスティック回帰分析を行った.ロジスティック回帰分析の結果.DMF歯の有無による2群比較において統計学的に有意であった変数は,性別(OR1.325,95%CI1.099-1.596,p=0.003),フッ化物歯面塗布経験の有無(OR0.765,95%CI0.634-0.924.p=0.005).食生活に対する自己効力感(OR0.813,95%CI0.672-0.982,p=0.032)および不規則な食事時間(OR1.250,95%CI1.005-1.554,p=0.045)の4項目であった.以上より,DMF歯の有無と食生活に対する自己効力感との間に有意な関連があった.

    DOI: 10.5834/jdh.62.4_403

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    その他リンク: http://search.jamas.or.jp/link/ui/2012333656

  • 早食いの是正に対するパンフレットと食事記録法の効果.

    竹内倫子, 江國大輔, 友藤孝明, 森田 学

    口腔衛生学会雑誌   62 ( 2 )   218 - 218   2012年

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 小学生における永久歯の萠出状況の報告

    横井 彩, 山中 玲子, 江國 大輔, 丸山 貴之, 入江 浩一郎, 遠藤 康正, 水谷 慎介, 友藤 孝明, 森田 学

    岡山歯学会雑誌   30 ( 2 )   87 - 87   2011年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 耳鼻咽喉科病棟看護師を対象に口腔ケアに関するアンケートの実施とその報告

    志茂 加代子, 岡崎 惠子, 三浦 留美, 山中 玲子, 水川 展吉, 江国 大輔, 丸山 貴之, 横井 彩, 仲田 直樹, 武田 斉子, 喜田 沙音里, 森田 学

    岡山歯学会雑誌   30 ( 2 )   82 - 82   2011年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • Relationships Between Eating Habits and Periodontal Condition in University Students 国際誌

    Takaaki Tomofuji, Michiko Furuta, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

    JOURNAL OF PERIODONTOLOGY   82 ( 12 )   1642 - 1649   2011年12月

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    記述言語:英語   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Being overweight is a risk factor for peri odontitis. Unhealthy eating habits, which can induce overweight, may be involved in the development of periodontitis in young people. The present study aims to examine the relationships among overweight, eating habits, and the periodontal condition in Japanese university students.
    Methods: We conducted a cross-sectional study of 801 university students (413 males and 388 females; age range: 18 to 25 years). Patients were classified as underweight (body mass index [BMI] &lt; 18.5 kg/m(2)), normal weight (BMI of 18.5 to 22.9 kg/m(2)), and overweight (BMI &gt;= 23 kg/m(2)). Students completed a questionnaire including items related to eating habits and underwent oral health examinations. Patients with a community periodontal index (CPI) of 0 to 2 were considered controls, and patients with a CPI &gt; 2 were considered to have periodontitis.
    Results: The prevalence of underweight, normal weight, and overweight patients was 21%, 62%, and 17%, respectively. In overweight patients, the periodontitis risk was increased by the frequent consumption of fatty foods (adjusted odds ratio: 2.3; 95% confidence interval: 1.1 to 5.2; P &lt; 0.05) and reduced by the frequent consumption of vegetables (adjusted odds ratio: 0.2; 95% confidence interval: 0.1 to 0.7; P &lt; 0.01). In underweight and normal-weight groups, eating habits did not differ significantly according to the presence of periodontitis.
    Conclusions: In overweight students, the frequent consumption of fatty foods and infrequent consumption of vegetables were associated with an increased risk of periodontitis. In underweight and normal-weight students, eating habits had little effect on the periodontal condition. J Periodontol 2071; 82:1642-1649.

    DOI: 10.1902/jop.2011.110061

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  • Hydrogen-rich water attenuates experimental periodontitis in a rat model 国際誌

    Kenta Kasuyama, Takaaki Tomofuji, Daisuke Ekuni, Naofumi Tamaki, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Manabu Morita

    JOURNAL OF CLINICAL PERIODONTOLOGY   38 ( 12 )   1085 - 1090   2011年12月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Aim: Reactive oxygen species (ROS) contribute to the development of periodontitis. As molecular hydrogen can act as a scavenger of ROS, we examined the effects of treatment with hydrogen-rich water on a rat model of periodontitis.
    Material & Methods: A ligature was placed around the maxillary molars for 4 weeks to induce periodontitis, and the animals were given drinking water with or without hydrogen-rich water.
    Results: The rats with periodontitis which were treated with pure water showed a time-dependent increase in serum ROS level. Compared with the rats without periodontitis, the periodontitis-induced rats which were given pure water also showed polymorphonuclear leucocyte infiltration and alveolar bone loss at 4 weeks. Hydrogen-rich water intake inhibited an increase in serum ROS level and lowered expression of 8-hydroxydeoxyguanosine and nitrotyrosine in the periodontal tissue at 4 weeks. Such conditions prevented polymorphonuclear leucocyte infiltration and osteoclast differentiation following periodontitis progression. Furthermore, inflammatory signalling pathways, such as mitogen-activated protein kinases, were less activated in periodontal lesions from hydrogen-rich water-treated rats as compared with pure water-treated rats.
    Conclusion: Consuming hydrogen-rich water might be beneficial in suppressing periodontitis progression by decreasing gingival oxidative stress.

    DOI: 10.1111/j.1600-051X.2011.01801.x

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  • Periodontal treatment decreases plasma oxidized LDL level and oxidative stress 国際誌

    Naofumi Tamaki, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Manabu Morita

    CLINICAL ORAL INVESTIGATIONS   15 ( 6 )   953 - 958   2011年12月

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    記述言語:英語   出版者・発行元:SPRINGER HEIDELBERG  

    Periodontitis induces excessive production of reactive oxygen species in periodontal lesions. This may impair circulating pro-oxidant/anti-oxidant balance and induce the oxidation of low-density lipoprotein (LDL) in blood. The purpose of this study was to monitor circulating oxidized LDL and oxidative stress in subjects with chronic periodontitis following non-surgical periodontal treatment. Plasma levels of oxidized LDL and oxidative stress in 22 otherwise healthy non-smokers with chronic periodontitis (mean age 44.0 years) were measured at baseline and at 1 and 2 months after non-surgical periodontal treatment. At baseline, chronic periodontitis patients had higher plasma levels of oxidized LDL and oxidative stress than healthy subjects (p &lt; 0.001). Periodontal treatment was associated with a significant reduction in plasma levels of oxidized LDL (oxLDL)(p &lt; 0.001) and oxidative stress (p &lt; 0.001). At 2 months after periodontal treatment, the degree of change in the oxLDL was positively correlated with that in the oxidative stress (r = 0.593, p = 0.004). These observations indicate that periodontitis patients showed higher levels of circulating oxLDL and oxidative stress than healthy subjects. In addition, improved oral hygiene and non-surgical periodontal treatment were effective in decreasing oxLDL, which was positively associated with a reduction in circulating oxidative stress.

    DOI: 10.1007/s00784-010-0458-y

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  • Stage of hepatocellular carcinoma is associated with periodontitis 国際誌

    Naofumi Tamaki, Akinobu Takaki, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Daisuke Ekuni, Tetsuya Yasunaka, Kazuhide Yamamoto, Manabu Morita

    JOURNAL OF CLINICAL PERIODONTOLOGY   38 ( 11 )   1015 - 1020   2011年11月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Aim: Periodontitis induces overproduction of reactive oxygen species (ROS). This state increases circulating ROS levels and may affect hepatocellular carcinoma (HCC). The Japan Integrated Stage (JIS) score is a novel staging system for HCC. The objective of the present study was to compare JIS scores in HCC patients with and without periodontitis.
    Material and Methods: We recruited 64 HCC patients comprising 31 chronic periodontitis subjects (HCC + P) and 33 periodontally healthy controls (HCC + H). Their JIS scores were recorded. Serum levels of reactive oxygen metabolites (ROM) from HCC + P, HCC + H and healthy age-and gender-matched subjects with healthy gingiva (control, n = 15) were also assessed for circulating ROS levels.
    Results: The HCC + P and HCC + H groups had similar body mass index, habitual drinking and tobacco exposure data. The HCC + P group showed higher JIS scores than the HCC + H group (p = 0.027). Both the HCC + P and HCC + H groups had higher serum levels of ROM than controls (p &lt; 0.001), while serum levels of ROM in the HCC + P group were a further 25.8% higher than those in the HCC + H group (p &lt; 0.001).
    Conclusion: HCC patients with periodontitis had higher JIS score and circulating ROS level than HCC patients without periodontitis.

    DOI: 10.1111/j.1600-051X.2011.01777.x

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  • Relationships between periodontal inflammation, lipid peroxide and oxidative damage of multiple organs in rats

    Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takayuki Maruyama, Tatsuo Yamamotto, Tatsuo Watanabe, Manabu Morita

    BIOMEDICAL RESEARCH-TOKYO   32 ( 5 )   343 - 349   2011年10月

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    記述言語:英語   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    Gingival response to periodontal inflammation generates excessive lipid peroxide and such a condition may augment systemic health through increased circulating lipid peroxide. The purpose of the present study was to investigate whether the generation of lipid peroxide in periodontal inflammation could induce tissue injury in the liver, heart, kidney and brain using a rat model. Twelve Wistar rats (8 week-old male) were divided into 2 groups: the periodontal inflammation group, receiving topical application of lipopolysaccharide and proteases to the gingival sulcus for 4 weeks, and the control group using instead pyrogen-free water. After blood samples were collected, specimens from the brain, heart, liver and kidney were resected to determine the concentration of 8-hydroxydeoxyguanosine (an indicator of oxidative DNA damage). Gingival and serum levels for hexanoyl-lysine were measured to evaluate lipid peroxide. Administration of lipopolysaccharide and proteases induced periodontal inflammation, with increasing gingival and serum levels of hexanoyl-lysine. The level of 8-hydroxydeoxyguanosine increased 2.27, 2.01, 1.49 and 1.40 times in mitochondrial DNA from the liver, heart, kidney and brain of rats with periodontal inflammation, respectively. The results reveal that excessive production of lipid peroxide following periodontal inflammation is involved in oxidative DNA damage of the brain, heart, liver and kidney.

    DOI: 10.2220/biomedres.32.343

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  • Relationship between impacts attributed to malocclusion and psychological stress in young Japanese adults 国際誌

    Daisuke Ekuni, Michiko Furuta, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Takashi Murakami, Takashi Yamashiro, Toshio Ogura, Manabu Morita

    EUROPEAN JOURNAL OF ORTHODONTICS   33 ( 5 )   558 - 563   2011年10月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Identifying risk factors is important to prevent a wide range of health-damaging behaviours and to improve the quality of life of young people. The aim of this study was to investigate the relationship between impacts on daily performance attributed to malocclusion and psychological stress in healthy young Japanese adults.
    Medical and oral health data were collected during a cross-sectional examination conducted by the Health Service Center of Okayama University. Systemically healthy non-smoking students aged 18 and 19 years (n = 641; 329 males and 312 females) were included. Malocclusion was defined using a modified version of the Index of Orthodontic Treatment Need (IOTN). The impacts on daily performance attributed to malocclusion and psychological stress were assessed using self-reported questionnaires, the condition-specific oral impacts on daily performances (CS-OIDP), and the Hopkins Symptoms Checklist. Mann-Whitney U- and chi-square tests and structural equation modelling (SEM) were used for statistical analysis.
    Forty per cent of subjects had a malocclusion (n = 255). Subjects with impacts on daily performance had a significantly higher prevalence of malocclusion than those without impacts (P &lt; 0.001). SEM showed that psychological stress, especially interpersonal sensitivity and depression, was significantly correlated with CS-OIDP and malocclusion. Negative impacts on daily performance attributed to malocclusion may contribute to psychological stress in young Japanese adults.

    DOI: 10.1093/ejo/cjq121

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  • Occlusal disharmony increases amyloid-β in the rat hippocampus.

    Ekuni D, Tomofuji T, Irie K, Azuma T, Endo Y, Kasuyama K, Morita M

    Neuromolecular Med   13 ( 3 )   197 - 203   2011年9月

  • 硫化水素とLPSの混合塗布による破骨細胞分化への影響

    入江 浩一郎, 江國 大輔, 友藤 孝明, 遠藤 康正, 粕山 健太, 八重垣 健, 森田 学

    日本歯周病学会会誌   53 ( 秋季特別 )   112 - 112   2011年9月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • Effects of exercise training on gingival oxidative stress in obese rats 国際誌

    Tetsuji Azuma, Takaaki Tomofuji, Yasumasa Endo, Naofumi Tamaki, Daisuke Ekuni, Koichiro Irie, Kenta Kasuyama, Tomo Kato, Manabu Morita

    ARCHIVES OF ORAL BIOLOGY   56 ( 8 )   768 - 774   2011年8月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: The purpose of the present study was to investigate the effects of exercise training on serum reactive oxygen species (ROS) level and gingival oxidative stress in obese rats fed a high-fat diet.
    Design: Rats were divided into three groups (n = 14/group): one control group (fed a regular diet) and two experimental groups (fed a high-fat diet with and without exercise training [treadmill: 5 days/week]). The rats were sacrificed at 4 or 8 weeks. The level of serum reactive oxidative metabolites (ROM) was measured as an indicator of circulating ROS. The level of 8-hydroxydeoxyguanosine (8-OHdG) and reduced-form glutathione (GSH)/oxidised-form glutathione (GSSG) ratio were determined to evaluate gingival oxidative stress.
    Results: The obese rats fed a high-fat diet without exercise training showed higher serum ROM levels [Carratelli Units (CARR U)] (mean +/- SD; 413 +/- 64) than the control (333 +/- 12) at 4 weeks (p = 0.023). Such a condition resulted in higher 8-OHdG levels (ng/mg mtDNA) (0.97 +/- 0.18) (p &lt; 0.05) and a lower GSH/GSSG ratio (17.0 +/- 3.1) (p &lt; 0.05) in gingival tissues, compared to the control (0.55 +/- 0.13 for 8-OHdG and 23.6 +/- 5.8 for GSH/GSSG ratio) at 8 weeks. In addition, the obese rats fed a high-fat diet with exercise training showed lower serum ROM (623 +/- 103) (p&lt;0.001) and gingival 8-OHdG levels (0.69 +/- 0.17) (p = 0.012) than those without exercise training (1105 95 for ROM and 0.55 +/- 0.13 for 8-OHdG) at 8 weeks.
    Conclusions: Obesity prevention by exercise training may effectively suppress gingival oxidative stress by decreasing serum ROS in rats. (C) 2011 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.archoralbio.2011.01.008

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  • 早食いの主観的評価と客観的評価の関連性

    江國 大輔, 古田 美智子, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   61 ( 4 )   500 - 500   2011年8月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 歯周炎の状態と肝癌のステージとの関連についての研究

    玉木 直文, 友藤 孝明, 遠藤 康正, 粕山 健太, 江國 大輔, 森田 学

    口腔衛生学会雑誌   61 ( 4 )   453 - 453   2011年8月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • 小学生における視力と口唇閉鎖力の関係(Relationship between Visual Acuity and Labial Closure Force in Japanese Elementary School Children)

    山中 玲子, アクター・ラヘナ, 古田 美智子, 江國 大輔, 江崎 光恵, 山本 龍生, 岡崎 好秀, 丸山 貴之, 横井 彩, 森田 学

    口腔衛生学会雑誌   61 ( 3 )   288 - 294   2011年7月

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    記述言語:英語   出版者・発行元:(一社)日本口腔衛生学会  

    口唇閉鎖力は、主に口輪筋の機能を反映している。口輪筋は発音や咀嚼などさまざまな口腔機能に関連している。口唇閉鎖力は咀嚼能力や不正咬合に関連がある。一方、裸眼視力1.0未満(&lt;20/20)の小学生は、日本においてこの20年間増え続けている。小学生の視力低下を予防することは、重要な課題である。視力は眼輪筋の強さに関連する可能性がある。眼輪筋は口輪筋とともに顔面筋の一つであり、両者ともに顔面神経の支配を受けており、頬筋などを介して機械的にもつながっており、協調して運動する。口輪筋の強さは、眼輪筋の強さを反映していると推測される。本研究では、口唇閉鎖力が視力に関連しているという仮説を立て、小学生の視力と口唇閉鎖力の関連性を横断的に検討することを目的とした。対象は岡山市内の7歳から12歳までの小学生396名(男子197名、女子199名)とした。口腔内状態と視力は、定期健康診断の結果を用いた。口唇閉鎖力は、LIP DE CUM/LDC-110(コスモ計器、東京)を用いて測定した。典型的な不正咬合を有する16名を除外し、高視力群(≧20/20)264名と低視力群(&lt;20/20)116名に分けた。統計分析には、Mann-Whitney U検定、χ2検定、ロジスティック回帰分析を用いた。高視力群では、口唇閉鎖力(p=0.024)と男子の割合(p=0.045)が有意に高く、年齢(p=0.001)とヘルマンの歯齢(p=0.002)が有意に低かった。性別、年齢、ヘルマンの歯齢で調整しても、低視力群では口唇閉鎖力が有意に低かった(OR 1.65、95% CI 1.04-2.64、p=0.004)。小学生において、視力と口唇閉鎖力には関連があることが示唆された。(著者抄録)

    DOI: 10.5834/jdh.61.3_288

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  • Sex Differences in Gingivitis Relate to Interaction of Oral Health Behaviors in Young People 国際誌

    Michiko Furuta, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Toshio Ogura, Manabu Morita

    JOURNAL OF PERIODONTOLOGY   82 ( 4 )   558 - 565   2011年4月

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    記述言語:英語   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Although many epidemiologic surveys have shown that gingivitis is more prevalent in males than in females, few studies have clearly explained what causes this difference. The objective of the present study is to explain the sex difference in gingivitis based on the interaction between oral health behaviors and related factors, such as knowledge, attitude, and lifestyle, in young people.
    Methods: The study was comprised of 838 subjects (440 males and 398 females), aged 18 and 19 years. Gingivitis was assessed by the percentage of bleeding on probing (%BOP). Additional information was collected regarding oral hygiene status, oral health behaviors, and related factors. Structural equation modeling was used to test pathways from these factors to %BOP. Multiple-group modeling was also conducted to test for sex differences.
    Results: Females had greater knowledge, a more positive attitude, a healthier lifestyle, and higher level of oral health behaviors than males. There were significant differences in the paths (i.e., from lifestyle, knowledge, and attitude to %BOP) through oral health behaviors and oral health status.
    Conclusions: Sex-based differences in gingivitis in young people can be explained by oral health behaviors and hygiene status, which are influenced by lifestyle, knowledge, and attitude. To prevent gingivitis, different approaches to males and females may be useful. J Periodontol 2011;82:558-565.

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  • Occlusal disharmony induces BDNF level in rat submandibular gland 国際誌

    Koichiro Irie, Daisuke Ekuni, Takaaki Tomofuji, Tetsuji Azuma, Yasumasa Endo, Kenta Kasuyama, Manabu Morita

    ARCHIVES OF ORAL BIOLOGY   56 ( 1 )   35 - 40   2011年1月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objectives: Brain-derived neurotrophic factor (BDNF), which is produced in rat submandibular gland, is one of the most abundant neurotrophins in the central nervous system. It is generally accepted that occlusal disharmony causes stress. The purpose of the present study was to investigate whether occlusal disharmony-induced chronic stress affects BDNF levels and morphology in rat submandibular gland.
    Design: Eight wks old male Wistar rats (n = 21) were randomly divided into three groups of 7 rats. In a control (C) group, the rats received no treatment for 8 wks. In a molar cusp-less (OD) group, maxillary molar cusps were cut off with a dental turbine at baseline and kept for 8 wks. In a molar cusp-less + recovered cusp (OR) group, maxillary molar cusps were cut off and then were recovered after 4 wks using resin material. After the experimental period, expression of BDNF mRNA and protein as well as histological findings were evaluated in the submandibular glands. The comparisons between the groups were made using the Mann-Whitney U test with Bonferroni correction.
    Results: The OD group showed a significant increase in submandibular gland BDNF mRNA and protein expression after 8 wks, and plasma adrenocorticotropic hormone and corticosterone levels increased in a time-dependent manner. There were no significant differences in BDNF expression in the submandibular glands and in levels of plasma adrenocorticotropic hormone and corticosterone between the OR and C groups.
    Conclusions: These results indicate that psychological stress induced by occlusal disharmony reversibly induces BDNF expression in the rat submandibular gland. (C) 2010 Elsevier Ltd. All rights reserved.

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  • Relationship Between Decrease of Erythrocyte Count and Progression of Periodontal Disease in a Rural Japanese Population 国際誌

    Tatsuo Yamamoto, Midori Tsuneishi, Michiko Furuta, Daisuke Ekuni, Manabu Morita, Yukio Hirata

    JOURNAL OF PERIODONTOLOGY   82 ( 1 )   106 - 113   2011年1月

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    記述言語:英語   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Limited research has been reported on the relationship between periodontal status and erythrocyte parameters. In the present study, longitudinal data from health checkups are used to clarify the relationship between periodontal disease progression and changes in parameters of erythrocytes.
    Methods: In this prospective study, a total of 120 subjects (35 men and 85 women; age range: 30 to 63 years) participated in a comprehensive health screening and dental checkup in 2006 and 2007. Medical examinations, including anthropometric and manometric measurements, blood-chemistry tests, and oral examinations, were performed. Subjects with periodontal disease progression (i.e., the progression group) were defined based on the presence of &gt;= 1 tooth demonstrating a longitudinal loss of periodontal attachment &gt;= 3 mm or tooth loss during the study period. The Wilcoxon signed-rank, chi(2), Fisher exact, and Mann-Whitney U tests and stepwise logistic regression analysis were used for statistical analyses.
    Results: The progression group comprised 30 subjects. A significant difference between the progression and non-progression groups was observed in changes of erythrocyte counts but not those of the body mass index, systolic and diastolic blood pressure, creatinine, fasting blood glucose, hemoglobin, hematocrit, triglycerides, high- and low-density lipoprotein cholesterol, total cholesterol, aspartate and alanine aminotransferase, and gamma-glutamyl transpeptidase. Stepwise logistic regression analysis showed that the progression of periodontal disease was associated with a change (year 2007 minus year 2006) of erythrocyte counts (adjusted odds ratio = 0.970; P = 0.009) after adjusting for age at baseline.
    Conclusion: Within the limitations of this study, the progression of periodontal disease is associated with a decrease in erythrocyte counts in a rural Japanese population. J Periodontol 2011;82:106-113.

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  • Supplementation of green tea catechins in dentifrices suppresses gingival oxidative stress and periodontal inflammation

    Takayuki Maruyama, Takaaki Tomofuji, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Daisuke Ekuni, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    ARCHIVES OF ORAL BIOLOGY   56 ( 1 )   48 - 53   2011年1月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: This study examined the effects of a dentifrice containing green tea catechins on gingival oxidative stress and periodontal inflammation using a rat model.
    Design: Twenty-four male Wister rats were randomly divided into four groups. The first group (Control group) received no treatment for 8 weeks. Periodontal inflammation was induced in the second group for 8 weeks. Periodontal inflammation was induced in the last two groups for 8 weeks and dentifrices with or without green tea catechins were topically applied to the gingival sulcus daily for 4 weeks prior to the end of the experimental period. Results: Rats that had experimental periodontal inflammation showed apical migration of the junctional epithelium, alveolar bone loss and inflammatory cell infiltration in the connective tissue subjacent to the junctional epithelium at 8 weeks, whilst the control group showed no pathologic changes. Topical application of a green tea catechin-containing dentifrice reduced inflammatory cell infiltration in the periodontal lesions to a greater degree than the control dentifrice at 8 weeks. The gingiva in which green tea catechin-containing dentifrice was applied also showed a lower level of expression of hexanoyl-lysine (a marker of lipid peroxidation), nitrotyrosine (a marker of oxidative protein damage), and tumour necrosis factor-a (an indicator of pro-inflammatory cytokines) at 8 weeks compared to gingiva in which the control dentifrice was applied.
    Conclusions: Adding green tea catechins to a dentifrice may contribute to prevention of periodontal inflammation by decreasing gingival oxidative stress and expression of pro-inflammatory cytokines. (C) 2010 Elsevier Ltd. All rights reserved.

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  • 新入生歯科健診からみえてきたこと

    江國大輔, 古田美智子, 入江浩一郎, 東 哲司, 児島 梓, 水谷慎介, 山中玲子, 友藤孝明, 森田 学, 山本龍生, 古賀 光, 岩崎良章, 清水幸登, 大西 勝, 小倉俊郎

    第41回中国・四国大学保健管理研究集会報告書   2011年

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  • Relationship between Malocclusion and Heart Rate Variability Indices in Young Adults A Pilot Study

    D. Ekuni, N. Takeuchi, M. Furuta, T. Tomofuji, M. Morita

    METHODS OF INFORMATION IN MEDICINE   50 ( 4 )   358 - 363   2011年

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    記述言語:英語   出版者・発行元:SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN  

    Objectives: Heart rate variability (HRV) has been used to assess sympathetic and parasympathetic modulation of heart rate. Chronic stress relates to reduced HRV. Malocclusion has effects on quality of life, which can lead to chronic stress. Therefore, we hypothesized that malocclusion, as chronic stress, may contribute to reduced HRV. The aim of this study was to investigate the relationship between malocclusion and HRV indices in healthy young adults.
    Methods: Thirty-seven non-smoking healthy subjects, aged 22 to 25 years, were examined. Malocclusion was defined by Angle classification. HRV indices included root mean square of successive differences, low frequency (LF), high frequency (HF) and ratio of LF to HE The effects of malocclusion on quality of life and mental health were assessed using self-reported questionnaires, the condition-specific Oral Impacts on Daily Performances index (CS-OIDP) and the Hopkins Symptoms Checklist (HSCL), respectively.
    Results: Significantly lower score of HF and higher heart rate (HR) level and CS-OIDR score were observed in subjects with malocclusion (n = 17) compared to those in the control subjects (n = 20) (P&lt;0.05). There was a positive correlation between HR and score of "anxiety" in HSCL (P &lt; 0.05).
    Conclusions: The data showed an association between malocclusion and lower HRV. Based on our results, orthodontic treatment might contribute not only to improvement of oral esthetic and functional problems but also to improvement of stress and HRV indices.

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  • A new portable monitor for measuring odorous compounds in oral, exhaled and nasal air 国際誌

    Naofumi Tamaki, Kenta Kasuyama, Mitsue Esaki, Takara Toshikawa, Shun-Ichi Honda, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    BMC ORAL HEALTH   11 ( 1 )   15 - 15   2011年

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    記述言語:英語   出版者・発行元:BIOMED CENTRAL LTD  

    Background: The B/B Checker(R), a new portable device for detecting odorous compounds in oral, exhaled, and nasal air, is now available. As a single unit, this device is capable of detecting several kinds of gases mixed with volatile sulfur compounds (VSC) in addition to other odorous gasses. The purpose of the present study was to evaluate the effectiveness of the B/B Checker(R) for detecting the malodor level of oral, exhaled, and nasal air.
    Methods: A total of 30 healthy, non-smoking volunteers (16 males and 14 females) participated in this study. The malodor levels in oral, exhaled, and nasal air were measured using the B/B Checker(R) and by organoleptic test (OT) scores. The VSCs in each air were also measured by gas chromatography (GC). Associations among B/B Checker(R) measurements, OT scores and VSC levels were analyzed using Spearman correlation coefficients. In order to determine the appropriate B/B Checker(R) level for screening subjects with malodor, sensitivity and specificity were calculated using OT scores as an identifier for diagnosing oral malodor.
    Results: In oral and nasal air, the total VSC levels measured by GC significantly correlated to that measured by the B/B Checker(R). Significant correlation was observed between the results of OT scores and the B/B Checker(R) measurements in oral (r = 0.892, p &lt; 0.001), exhaled (r = 0.748, p &lt; 0.001) and nasal air (r = 0.534, p &lt; 0.001). The correlation between the OT scores and VSC levels was significant only for oral air (r = 0.790, p &lt; 0.001) and nasal air (r = 0.431, p = 0.002); not for exhaled air (r = 0.310, p = 0.096). When the screening level of the B/B Checker(R) was set to 50.0 for oral air, the sensitivity and specificity were 1.00 and 0.90, respectively. On the other hand, the screening level of the B/B Checker(R) was set to 60.0 for exhaled air, the sensitivity and specificity were 0.82 and 1.00, respectively.
    Conclusion: The B/B Checker(R) is useful for objective evaluation of malodor in oral, exhaled and nasal air and for screening subjects with halitosis.

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  • Antioxidants and prevention of periodontal disease.

    Global Research Network   2011年

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  • Relationship between Malocclusion and Heart Rate Variability Indices in Young Adults A Pilot Study

    D. Ekuni, N. Takeuchi, M. Furuta, T. Tomofuji, M. Morita

    METHODS OF INFORMATION IN MEDICINE   50 ( 4 )   358 - 363   2011年

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    記述言語:英語   出版者・発行元:SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN  

    Objectives: Heart rate variability (HRV) has been used to assess sympathetic and parasympathetic modulation of heart rate. Chronic stress relates to reduced HRV. Malocclusion has effects on quality of life, which can lead to chronic stress. Therefore, we hypothesized that malocclusion, as chronic stress, may contribute to reduced HRV. The aim of this study was to investigate the relationship between malocclusion and HRV indices in healthy young adults.
    Methods: Thirty-seven non-smoking healthy subjects, aged 22 to 25 years, were examined. Malocclusion was defined by Angle classification. HRV indices included root mean square of successive differences, low frequency (LF), high frequency (HF) and ratio of LF to HE The effects of malocclusion on quality of life and mental health were assessed using self-reported questionnaires, the condition-specific Oral Impacts on Daily Performances index (CS-OIDP) and the Hopkins Symptoms Checklist (HSCL), respectively.
    Results: Significantly lower score of HF and higher heart rate (HR) level and CS-OIDR score were observed in subjects with malocclusion (n = 17) compared to those in the control subjects (n = 20) (P&lt;0.05). There was a positive correlation between HR and score of "anxiety" in HSCL (P &lt; 0.05).
    Conclusions: The data showed an association between malocclusion and lower HRV. Based on our results, orthodontic treatment might contribute not only to improvement of oral esthetic and functional problems but also to improvement of stress and HRV indices.

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  • Oral malodorous compound induces osteoclast differentiation without receptor activator of nuclear factor κB ligand. 国際誌

    Ii H, Imai T, Yaegaki K, Irie K, Ekuni D, Morita M

    J Periodontol.   81 ( 11 )   1691 - 1697   2010年11月

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  • Relationship between periodontal condition and arterial properties in an adult population in Japan

    M. Furuta, T. Tomofuji, D. Ekuni, N. Tamaki, T. Yamamoto, T. Azuma, K. Irie, Y. Endo, S. Yamada, M. Morita

    ORAL DISEASES   16 ( 8 )   781 - 787   2010年11月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Objective:
    This study addressed the relationship between periodontal condition and second derivative of the finger photoplethysmogram (SDPTG) in Japanese adults.
    Subject and methods:
    The Community Periodontal Index (CPI) and SDPTG were recorded in 415 subjects (mean age: 44.0 years). For assessing SDPTG, we mainly focused on the ratio of the absolute value of the height of the early negative &apos;b&apos; wave and ratio of the late re-decreasing &apos;d&apos; wave to the height of the initial positive &apos;a&apos; wave, namely the b/a and d/a ratios.
    Results:
    The CPI score was positively correlated with the b/a ratio (P &lt; 0.001), and negatively correlated with the d/a ratio (P &lt; 0.001). Logistic regression analysis showed that subjects with CPI scores &gt;= 3 were more likely to have a higher level (male &gt;-0.69, female &gt;-0.64) of b/a ratio (Odds ratio = 1.7, P = 0.026) and lower level (male &lt;-0.29, female &lt;-0.32) of d/a ratio (Odds ratio = 2.2, P = 0.001) than those with CPI scores 0-2, after adjusting for age, gender, smoking status, pulse rate and presence of hypertension.
    Conclusion:
    There was a statistical association between the CPI scores and SDPTG indices in Japanese adults.

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  • 小学生における視力と口唇閉鎖力の関係

    山中 玲子, 古田 美智子, 江崎 光恵, 江國 大輔, 丸山 貴之, 岡崎 好秀, 森田 学

    口腔衛生学会雑誌   60 ( 5 )   606 - 606   2010年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • Relationship between the prognosis of periodontitis and occlusal force during the maintenance phase - a cohort study

    N. Takeuchi, D. Ekuni, T. Yamamoto, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   45 ( 5 )   612 - 617   2010年10月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Background and Objective:
    Few studies have longitudinally investigated the relationship between periodontal disease progression and occlusal factors in individual subjects during the maintenance phase of periodontal therapy. The aim of this cohort study was to investigate the relationship between biting ability and the progression of periodontal disease in the maintenance phase.
    Material and Methods:
    A total of 194 patients were monitored for 3 years during the maintenance phase of periodontal therapy. The subjects with disease progression (Progress group) were defined based on the presence of &gt;= 2 teeth demonstrating a longitudinal loss of proximal attachment of &gt;= 3 mm or tooth-loss experience as a result of periodontal disease during the study period. The subjects with high occlusal force were diagnosed as men who showed an occlusal force of more than 500 N and women who showed an occlusal force of more than 370 N. The association between biting ability and the progression of periodontitis was investigated using logistic regression analysis.
    Results:
    There were 83 subjects in the Progress group and 111 subjects in the Non-progress group. A backward, stepwise logistic regression model showed that the progression of periodontal disease was significantly associated with the presence of one or more teeth with a high clinical attachment level (CAL) of &gt;= 7 mm (odds ratio: 2.397; 95% confidence interval: 1.306-4.399) ( p = 0.005) and low occlusal force (odds ratio: 2.352; 95% confidence interval: 1.273-4.346) ( p = 0.006).
    Conclusion:
    The presence of one or more teeth with a high CAL of &gt;= 7 mm and low occlusal force might be possible risk factors for periodontal progression in the maintenance phase of periodontal therapy.

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  • 歯周状態と加速度脈波との関連性についての検討

    遠藤 康正, 友藤 孝明, 江國 大輔, 玉木 直文, 山本 龍生, 東 哲司, 入江 浩一郎, 森田 学

    日本歯周病学会会誌   52 ( 秋季特別 )   97 - 97   2010年9月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 頭頸部がん患者における口腔内の状態と血清アルブミン値の関係

    丸山 貴之, 山中 玲子, 横井 彩, 古田 美智子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   60 ( 4 )   461 - 461   2010年8月

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  • 大学新入生における歯肉炎の性差についての検討

    古田 美智子, 江國 大輔, 入江 浩一郎, 東 哲司, 加藤 朋, 山田 小百合, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   60 ( 4 )   364 - 364   2010年8月

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  • 運動による肥満予防がラット歯周組織に及ぼす影響

    東 哲司, 入江 浩一郎, 遠藤 康正, 粕山 健太, 加藤 朋, 江國 大輔, 玉木 直文, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   60 ( 4 )   414 - 414   2010年8月

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  • 咬合異常は顎下腺BDNFに影響を及ぼす

    入江 浩一郎, 江國 大輔, 友藤 孝明, 遠藤 康正, 東 哲司, 粕山 健太, 森田 学

    日本抗加齢医学会総会プログラム・抄録集   10回   236 - 236   2010年5月

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  • Experimental Periodontitis Induces Gene Expression of Proinflammatory Cytokines in Liver and White Adipose Tissues in Obesity

    Yasumasa Endo, Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    JOURNAL OF PERIODONTOLOGY   81 ( 4 )   520 - 526   2010年4月

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    記述言語:英語   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Recent studies indicated that periodontitis induces systemic low-grade inflammation. The increase in systemic low-grade inflammation induced by periodontitis may alter the effects of obesity on the production of inflammatory molecules, including C-reactive protein (CRP), interleukin (IL)-6, and tumor necrosis factor-alpha (TNF-alpha), in the liver and white adipose tissue (WAT). The purpose of the present study is to investigate the effects of periodontitis on the expression of proinflammatory cytokines in the liver and WAT in obese Zucker rats.
    Methods: Obese Zucker rats and their lean litter mates were divided into four groups of six rats each: lean Zucker rats without periodontitis (control group), lean Zucker rats with periodontitis (periodontitis group), obese Zucker rats without periodontitis (obesity group), and obese Zucker rats with periodontitis (combination group). Periodontitis was ligature induced for 4 weeks in the periodontitis and combination groups, whereas the other groups were left unligated.
    Results: At 4 weeks, the gene expression for CRP, IL-6, and TNF-alpha in the liver and CRP and IL-6 in the WAT of combination groups was significantly higher than in each of the three groups. Serum TNF-alpha in the periodontitis and obesity groups was significantly higher than in the control group. Serum CRP and TNF-alpha in the combination group was significantly higher than in each of the three groups.
    Conclusion: Systemic low-grade inflammation after experimental periodontitis was associated with increased gene expression for hepatic levels of TNF-alpha and CRP and adipose tissue levels of IL-6 and CRP in the obese-rat model. J Periodontol 2010;81:520-526.

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  • Effects of periodontitis on aortic insulin resistance in an obese rat model

    Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Tetsuji Azuma, Naofumi Tamaki, Toshihiro Sanbe, Yasumasa Endo, Tatsuo Yamamoto, Takashi Nishida, Manabu Morita

    LABORATORY INVESTIGATION   90 ( 3 )   348 - 359   2010年3月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    The combination of obesity and its associated risk factors, such as insulin resistance and inflammation, results in the development of atherosclerosis. However, the effects of periodontitis on atherosclerosis in an obese body remain unclear. The aim of the study was to investigate the effects of ligature-induced periodontitis in Zucker fatty rats on initiation of atherosclerosis by evaluating aortic insulin resistance. Zucker fatty rats (n = 24) were divided into two groups. In the periodontitis group, periodontitis was ligature-induced for 4 weeks, whereas the control group was left unligated. After the 4-week experimental period, descending aorta was used for measuring the levels of lipid deposits, immunohistochemical analysis, and evaluation of gene expression. Levels of serum C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-alpha), and insulin were also measured. Rats in the periodontitis group had significantly enhanced lipid deposits in the aorta, but not in the control group. Expression of suppressor of cytokine signaling 3, vascular cell adhesion molecule 1, reactive oxygen species, nitrotyrosine, and endothelin-1 in the periodontitis group was more intense than that in the control group. Significantly decreased levels of phosphatidylinositol 3-kinase (Pi3k) catalytic beta-polypeptide (Pi3kcb), Pi3kp85, and insulin receptor substrate 1 and 2 were observed in the periodontitis group. Levels of serum CRP and TNF-alpha were significantly increased in the periodontitis group. Under insulin-stimulated conditions, aorta in the periodontitis group altered the Akt phosphorylation. Periodontitis in obesity induced the initial stage of atherosclerosis and disturbed aortic insulin signaling.

    DOI: 10.1038/labinvest.2009.141

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  • Effects of topical application of lipopolysaccharide and proteases on hepatic injury induced by high-cholesterol diet in rats

    T. Yamamoto, T. Tomofuji, N. Tamaki, D. Ekuni, T. Azuma, T. Sanbe

    JOURNAL OF PERIODONTAL RESEARCH   45 ( 1 )   129 - 135   2010年2月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Background and Objective: Topical application of lipopolysaccharide and proteases to the gingival sulcus induced not only periodontal inflammation but also detectable liver changes in rats fed a normal diet. However, these changes in the liver were not sufficient to induce pathological consequences. The purpose of the present study was to investigate whether gingival inflammation-induced liver change would have more dramatic pathological consequences in rats fed a high-cholesterol diet compared with the effect of the high-cholesterol diet alone.
    Material and Methods: Twenty-four male Wistar rats were divided into four groups. During an 8 week experimental period, two groups were fed a normal diet and the other two were fed a high-cholesterol diet containing 1% cholesterol (w/w) and 0.5% cholic acid (w/w). Four weeks prior to the end of the experimental period, one of each of the dietary groups received daily topical application of lipopolysaccharide and proteases to the gingival sulcus, while the other was treated with pyrogen-free water.
    Results: In the rats without application of lipopolysaccharide and proteases, the serum level of hexanoyl-lysine, scores of steatosis and inflammation, and concentration of 8-hydroxydeoxyguanosine in liver of rats fed a high-cholesterol diet were higher than in those fed a normal diet. In rats fed a high-cholesterol diet, the scores of steatosis and inflammation and the concentration of 8-hydroxydeoxyguanosine in the liver of rats with application of lipopolysaccharide and proteases were higher than in those without.
    Conclusion: In a rat model, application of lipopolysaccharide and proteases to the gingival sulcus augmented the effect of a high-cholesterol diet on steatosis, inflammation and oxidative damage in the liver.

    DOI: 10.1111/j.1600-0765.2009.01212.x

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  • Effects of mechanical stimulation by a powered toothbrush on the healing of periodontal tissue in a rat model of periodontal disease

    D. Ekuni, R. Yamanaka, T. Yamamoto, M. Miyauchi, T. Takata, T. Watanabe

    JOURNAL OF PERIODONTAL RESEARCH   45 ( 1 )   45 - 51   2010年2月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Background and Objective: Elimination of pathogens is the main aim of periodontal treatment; however, modulation of the host immune response should also be considered. This study aimed to evaluate the effects of mechanical stimulation on periodontal healing in rats.
    Material and Methods: Before starting the experiment, lipopolysaccharide and proteases were applied once a day, for 4 wk, to both maxillary first molars of 30 rats to induce periodontal disease, and the application was stopped at the end of the 4-wk period. The experiment started immediately following this pretreatment. In the experiment, the left palatal gingiva was stimulated once daily using a powered toothbrush and the right gingiva served as a control (no mechanical stimulation). Pathological changes, and proliferation and cell death in periodontal tissues, were evaluated histometrically and immunohistochemically at baseline (0 wk), and at 1 and 3 wk of stimulation.
    Results: The control showed a reduction of polymorphonuclear leukocyte infiltration in connective tissue and an increase in the numbers of gingival and periodontal ligament fibroblasts. Mechanical stimulation reduced polymorphonuclear leukocyte infiltration and the area of destroyed collagen in connective tissue, and increased the number of gingival fibroblasts; however, it had no effect on alveolar bone and root resorption or on the number of periodontal ligament fibroblasts.
    Conclusion: Mechanical stimulation accelerated the healing of gingival inflammation by reducing the infiltration of polymorphonuclear leukocytes and enhancing fibroblast proliferation and collagen synthesis.

    DOI: 10.1111/j.1600-0765.2009.01195.x

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  • Imbalance of oxidative/anti-oxidative status induced by periodontitis is involved in apoptosis of rat submandibular glands 国際誌

    Daisuke Ekuni, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takaaki Tomofuji, Manabu Morita

    ARCHIVES OF ORAL BIOLOGY   55 ( 2 )   170 - 176   2010年2月

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    記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Objective: Epidemiologic studies suggest a relationship between periodontitis and salivary gland dysfunction. A rat periodontitis model was used to investigate whether a causal relationship exists between periodontitis and pathological changes of submandibular glands.
    Design: Fourteen male Wistar rats (8 weeks old) were divided into two groups (n = 7/group): a control group and periodontitis group. Periodontitis was induced by ligature placement around the mandibular first molars. Serum levels for reactive oxygen metabolites, antioxidant and tumour necrosis factor (TNF)-alpha were measured at baseline, 2 and 4 weeks. At 4 weeks, the levels of 8-hydroxydeoxyguanosine were determined to evaluate oxidative damage of submandibular glands. Expression of TNF-alpha mRNA and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) as well as histological findings were also evaluated in the submandibular glands.
    Results: The rats with experimental periodontitis showed increase in the levels of serum reactive oxygen metabolites and TNF-alpha, and a decrease of anti-oxidant power in a time-dependent manner. At 4 weeks, these rats also had significantly increased levels of 8-hydroxydeoxyguanosine and TNF-alpha, and increased number of TUNEL-positive cells and vacuolisation in the submandibular glands compared to the control rats.
    Conclusions: Imbalance of circulating oxidative/anti-oxidative status may be involved in vacuolisation and apoptosis of submandibular glands in the rat periodontitis model. (C) 2009 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.archoralbio.2009.11.013

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  • Relationship between periodontal status and components of metabolic syndrome in a rural Japanese population.

    Yamamoto T, Tsuneishi M, Furuta M, Koyama R, Ekuni D, Morita M, Hirata Y

    口腔衛生学会雑誌   60   96 - 103   2010年

  • ワークショップ「頭頸部がんチーム医療における口腔ケアの促進」と連携強化.

    山中玲子, 丸山貴之, 横井 彩, 山田小百合, 玉木直文, 江國大輔, 友藤孝明, 森田 学

    岡山歯学会雑誌   61   2010年

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  • メインテナンス期の歯周病患者における咬合力と歯周病進行との関係について

    竹内倫子, 江國大輔, 山本龍生, 森田 学

    岡山歯学会雑誌   29 ( 1 )   67 - 67   2010年

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 健康診査データにおけるメタボリックシンドロームと歯周炎の関係

    山本 龍生, 恒石 美登里, 古田 美智子, 小山 玲子, 江國 大輔, 森田 学, 平田 幸夫

    神奈川歯学   44 ( 抄録集 )   25 - 25   2009年12月

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    記述言語:日本語   出版者・発行元:神奈川歯科大学学会  

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  • Preventive Effects of a Cocoa-Enriched Diet on Gingival Oxidative Stress in Experimental Periodontitis

    Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Yasumasa Endo, Naofumi Tamaki, Toshihiro Sanbe, Jun Murakami, Tatsuo Yamamoto, Manabu Morita

    JOURNAL OF PERIODONTOLOGY   80 ( 11 )   1799 - 1808   2009年11月

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    記述言語:英語   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Oxidative stress affects the progression of periodontitis. Cocoa is a rich source of flavonoids with antioxidant properties, which could suppress gingival oxidative stress in periodontal lesions. The purpose of the present study was to investigate the effects of a cocoa-enriched diet on gingival oxidative stress in a rat-periodontitis model.
    Methods: In this 4-week study, rats were divided into three groups (n = 8/group): a control group (fed a regular diet) and two periodontitis groups (fed a regular diet or cocoa-enriched diet [10% of food intake]). Periodontitis was induced by ligature placement around the mandibular first molars. Serum levels for reactive oxygen metabolites were measured at baseline and 2 and 4 weeks. At 4 weeks, the levels of 8-hydroxydeoxyguanosine and reduced/oxidized glutathione ratio were determined to evaluate gingival oxidative damage and antioxidant status, respectively.
    Results: Rats with experimental periodontitis that were fed a regular diet showed an increase in the level of serum reactive oxygen metabolites in a time-dependent manner. These rats also had an increased 8-hydroxydeoxyguanosine level and decreased reduced/oxidized glutathione ratio in the gingival tissue, inducing alveolar bone loss and polymorphonuclear leukocyte infiltration. Although experimental periodontitis was induced in the rats fed a cocoa-enriched diet, they did not show impairments in serum reactive oxygen metabolite level and gingival levels for 8-hydroxydeoxyguanosine and reduced/oxidized glutathione ratio. Alveolar bone loss and polymorphonuclear leukocyte infiltration after ligature placement were also inhibited by cocoa intake.
    Conclusion: Consuming a cocoa-enriched diet could diminish periodontitis-induced oxidative stress, which, in turn, might suppress the progression of periodontitis. J Periodontol 2009;80:1799-1808.

    DOI: 10.1902/jop.2009.090270

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  • 急性期病院における頭頸部癌患者への口腔ケア

    山中 玲子, 小山 玲子, 江國 大輔, 古田 美智子, 丸山 貴之, 山本 龍生, 森田 学

    口腔衛生学会雑誌   59 ( 5 )   609 - 610   2009年10月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔衛生学会  

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  • Lipopolysaccharide-Induced Epithelial Monoamine Oxidase Mediates Alveolar Bone Loss in a Rat Chronic Wound Model 国際誌

    Daisuke Ekuni, James D. Firth, Tarun Nayer, Takaaki Tomofuji, Toshihiro Sanbe, Koichiro Irie, Tatsuo Yamamoto, Takashi Oka, Zhenzi Liu, Juergen Vielkind, Edward E. Putnins

    AMERICAN JOURNAL OF PATHOLOGY   175 ( 4 )   1398 - 1409   2009年10月

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    記述言語:英語   出版者・発行元:AMER SOC INVESTIGATIVE PATHOLOGY, INC  

    Reactive oxygen species (ROS) production is an antimicrobial response to pathogenic challenge that may, in the case of persistent infection, have deleterious effects on the tissue of origin. A rat periodontal disease model was used to study ROS-induced chronic epithelial inflammation and bone loss. Lipopolysaccharide (LPS) was applied for 8 weeks into the gingival sulcus, and histological analysis confirmed the onset of chronic disease. junctional epithelium was collected from healthy and diseased animals using laser-capture microdissection, and expression microarray analysis was performed. Of 19,730 genes changed in disease, 42 were up-regulated &gt;= 4-fold. Three of the top 10 LPS-induced genes, monoamine oxidase B (MAO/B) and flavin-containing monooxygenase 1 and 2, are implicated in ROS signaling. LPS-associated induction of the ROS mediator H(2)O(2) as well as MAO/B and tumor necrosis factor (TNF)-alpha levels were validated in the rat histological sections and a porcine junctional epithelial cell culture model. Topical MAO inhibitors significantly counteracted LPS-associated elevation of H(2)O(2) production and TNF-alpha expression in vivo and in vitro, inhibited disease-associated apical migration and proliferation of junctional epithelium and inhibited induced systemic H(2)O(2) levels and alveolar bone loss in vivo. These results suggest that LPS induces chronic wounds via elevated MAO/B-mediated increases in H(2)O(2) and TNF-alpha activity by epithelial cells and is further associated with more distant effects on systemic oxidative stress and alveolar bone loss. (Am J Pathol 2009,175:1398-1409; DOI: 10.2353/ajpath.2009.090108)

    DOI: 10.2353/ajpath.2009.090108

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  • Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis

    D. Ekuni, T. Tomofuji, T. Sanbe, K. Irie, T. Azuma, T. Maruyama, N. Tamaki, J. Murakami, S. Kokeguchi, T. Yamamoto

    JOURNAL OF PERIODONTAL RESEARCH   44 ( 4 )   434 - 442   2009年8月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Background and Objective:
    Periodontitis is a risk factor for the development of atherosclerosis. Recent studies indicate that oxidative mechanisms, including lipid peroxidation, are involved not only in periodontitis but also in atherosclerosis. Lipid peroxidation may play an important role in the pathogenesis of atherosclerosis, particularly during its earliest stages. The purpose of this study was to investigate the relationship between lipid peroxidation induced by periodontitis and the initiation of atherosclerosis.
    Material and Methods:
    Sixteen rats were randomly divided into two groups of eight rats each. Periodontitis was ligature-induced for 4 wk in the experimental group, whereas the control group was left untreated. After the experimental period, the mandibular first molar regions were resected and then subjected to histological analysis and measurement of hexanoyl-lysine expression as an indicator of lipid peroxidation. Descending aorta was used for measuring the levels of hexanoyl-lysine, reactive oxygen species and lipid deposits, and for real-time polymerase chain reaction microarray analysis. The level of hexanoyl-lysine was also measured in serum.
    Results:
    In the experimental group, the levels of hexanoyl-lysine in periodontal tissue and serum increased. Only aorta samples in the experimental group showed lipid accumulation, with increased expression of hexanoyl-lysine, reactive oxygen species and oxidative stress-related genes (including nitric oxide synthases 2 and 3), whereas the superoxide dismutase 1 gene level was down-regulated.
    Conclusion:
    In a ligature-induced periodontitis rat model, increased lipid peroxidation was found in serum and aorta as well as in periodontal tissue. Atherosclerosis-related gene expression and histological changes were also stimulated. Periodontitis-induced lipid peroxidation in the aorta may be involved in the early stage of atherosclerosis.

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  • Relation of dietary preference to bite force and occlusal contact area in Japanese children

    R. Yamanaka, R. Akther, M. Furuta, R. Koyama, T. Tomofuji, D. Ekuni, N. Tamaki, T. Azuma, T. Yamamoto, E. Kishimoto

    JOURNAL OF ORAL REHABILITATION   36 ( 8 )   584 - 591   2009年8月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    P&gt;The purpose of this study was to examine the relationship of dietary preference to bite force and occlusal contact area in Japanese elementary school children. A total of 348 children, aged 7-12 years, from two public elementary schools located in Okayama Prefecture, Japan, participated in the study. Clinical examination included decayed, missing and filled teeth (dmft and DMFT), and total numbers of deciduous and permanent teeth. Bite force and occlusal contact area were measured using a pressure-detecting sheet. Dietary preference was assessed using a questionnaire in which the answers were given in like/dislike form. Mann-Whitney U-test and multiple logistic regression analysis were applied to analyse the data. In multiple logistic regression analysis after adjustment for age, gender and total number of teeth present, children who liked cabbage and celery showed significantly higher bite force (P = 0 center dot 05 and P &lt; 0 center dot 01, respectively) than those who disliked these. Children who liked cabbage and celery also showed higher occlusal contact area (P &lt; 0 center dot 05 and P &lt; 0 center dot 01, respectively) than those who disliked these. The Japanese elementary school children who liked hard foods such as cabbage and celery showed higher bite force and higher occlusal contact area than those who disliked these foods. A positive attitude towards harder food items might contribute to healthy development of the masticatory apparatus.

    DOI: 10.1111/j.1365-2842.2009.01971.x

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  • Effects of Obesity on Gingival Oxidative Stress in a Rat Model 国際誌

    Takaaki Tomofuji, Tatsuo Yamamoto, Naofumi Tamaki, Daisuke Ekuni, Tetsuji Azuma, Toshihiro Sanbe, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Jun Murakami, Susumu Kokeguchi, Manabu Morita

    JOURNAL OF PERIODONTOLOGY   80 ( 8 )   1324 -