Updated on 2021/12/16

写真a

 
MORITA Manabu
 
Organization
Medicine, Dentistry and Pharmaceutical Sciences Professor
Position
Professor
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Degree

  • 歯学博士 ( 岡山大学 )

Research Interests

  • 予防歯科学

  • Preventive Dentistry

Research Areas

  • Life Science / Social dentistry

Education

  • Osaka University   歯学部   歯学科

    1976.4 - 1982.3

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Research History

  • - 岡山大学医歯薬学総合研究科 教授

    2008

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  • - Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2008

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  • Professor

    2000 - 2008

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  • Hokkaido University

    2000 - 2008

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Committee Memberships

  • 日本歯周病学会   理事  

    2021.5   

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  • 日本口腔衛生学会   監事  

    2021.5   

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  • 日本公衆衛生学会   評議員  

    2020.10   

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  • 日本口臭学会   理事長  

    2020.5   

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  • 岡山県8020推進事業評価会議   副委員長  

    2020.4   

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  • 岡山県歯科保健対策協議会   副委員長  

    2020.4   

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  • 厚労省「歯科健康診査推進事業」検討委員会   委員長  

    2020.4   

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  • 岡山市歯と口腔の健康づくり推進協議会   委員長  

    2020.4   

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  • 独立行政法人大学改革支援・学位授与評価 機構 国立大学教育研究評価委員会   委員  

    2020.4   

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  • 厚労省[歯周病予防に関する実証事業」委員会   委員長  

    2020.4   

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  • 日本産業衛生学会 中国地方会   幹事  

    2020.4   

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  • 日本老年歯科医学会   評議員  

    2020.1   

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  • 近畿中国四国口腔衛生学会   幹事  

    2020.1   

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  • 岡山歯学会   理事  

    2020.1   

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  • 北海道歯学会   評議員  

    2020.1   

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  • 日本産業衛生学会歯科保健部会   幹事  

    2020.1   

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Papers

  • Oral function, nutritional status and physical status in Japanese independent older adults. Reviewed International journal

    Nanami Sawada, Noriko Takeuchi, Daisuke Ekuni, Manabu Morita

    Gerodontology   2021.10

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    OBJECTIVES: To clarify the associations among oral status, nutritional status and physical status in Japanese independent older adults. BACKGROUND: It is important to focus on factors affecting physical status associated with life dysfunction, long-term care and mortality. However, there are very few reports of the associations among oral status, nutritional status and physical status. MATERIALS AND METHODS: Patients who visited the Preventive Dentistry Clinic at Okayama University Hospital from November 2017 to January 2019 participated in this cross-sectional study. Number of teeth, periodontal condition and oral function were recorded. Bacteria counts in tongue dorsum, oral wettability, tongue pressure, tongue and lip movement function [oral diadochokinesis (ODK)], masticatory ability, bite force and swallowing function were measured. Nutritional status was assessed by the Mini Nutritional Assessment. Physical frailty status and Elderly Status Assessment Set were also evaluated. These variables were analysed by structural equation modelling (SEM). RESULTS: Data from 203 patients were analysed (63 males and 140 females). Patients ranged in age from 60 to 93 years. The final model of the path diagram was completed by SEM. ODK was positively associated with nutritional status, and nutritional status was negatively associated with frailty. Age was associated with ODK, nutritional status and frailty. CONCLUSION: Based on the associations among age, ODK, nutritional status and frailty, maintaining tongue movement function may contribute to good nutritional status and physical status in Japanese independent older adults.

    DOI: 10.1111/ger.12593

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  • Association between Self-Reported Chewing Status and Glycemic Control in Japanese Adults. Reviewed International journal

    Komei Iwai, Tetsuji Azuma, Takatoshi Yonenaga, Daisuke Ekuni, Kazutoshi Watanabe, Akihiro Obora, Fumiko Deguchi, Takao Kojima, Manabu Morita, Takaaki Tomofuji

    International journal of environmental research and public health   18 ( 18 )   2021.9

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    This cross-sectional study investigated the relationship between self-reported chewing status and glycemic control in 30,938 Japanese adults who participated in health checkups. Chewing status was evaluated using a self-reported questionnaire. We defined high hemoglobin A1c (HbA1c) levels as a HbA1c level ≥6.5%; 692 (2.2%) respondents met this criterion. After adjusting for gender, age, smoking status, exercise habits, body mass index and eating speed, high HbA1c levels was found to be associated with male gender (odds ratio (OR), 1.568; 95% confidence interval (CI), 1.310 to 1.878; p < 0.001), older age (OR, 1.077; 95% CI, 1.068 to 1.087; p < 0.001), higher body mass index (OR, 1.246; 95% CI, 1.225 to 1.268; p < 0.001), current smoker status (OR, 1.566; 95% CI, 1.303 to 1.882; p < 0.001) and chewing difficulty (OR, 1.302; 95% CI, 1.065 to 1.591; p < 0.05). In conclusion, self-reported chewing difficulty was associated with high HbA1c levels in Japanese adults.

    DOI: 10.3390/ijerph18189548

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  • Self-Reported Diabetes Mellitus and Tooth Extraction Due to Periodontal Disease and Dental Caries in the Japanese Population. Reviewed International journal

    Seitaro Suzuki, Naoki Sugihara, Hideyuki Kamijo, Manabu Morita, Takayuki Kawato, Midori Tsuneishi, Keita Kobayashi, Yoshihiro Hasuike, Tamotsu Sato

    International journal of environmental research and public health   18 ( 17 )   2021.8

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    Diabetes mellitus is closely related to oral health. We aimed to determine the relationship between diabetes mellitus and tooth extraction due to periodontal disease and dental caries. Japan's second nationwide survey data collected from 4 June to 10 June 2018 was used to identify reasons for tooth extraction among patients aged > 40 years. General dentists collected information on patients who underwent tooth extraction procedures, and the presence of diabetes mellitus was determined through interviews. Multivariable logistic regression was performed to investigate the relationship between diabetes mellitus and the reasons for tooth extraction, including periodontal disease and dental caries. In total, 2345 dentists responded to the survey (response rate 44.8%). We analyzed data on 4625 extracted teeth from 3750 patients (1815 males and 1935 females). Among patients with self-reported diabetes mellitus, 55.4% had extractions due to periodontal disease compared to 46.7% of such extractions among those without self-reported diabetes mellitus. Self-reported diabetes mellitus was significantly associated with tooth extraction due to periodontal disease. No significant differences were observed in dental caries according to self-reported diabetes mellitus status. This study provides further evidence of a significant association between diabetes mellitus and tooth extraction due to periodontal disease.

    DOI: 10.3390/ijerph18179024

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  • Detection of Salivary miRNAs That Predict Chronic Periodontitis Progression: A Cohort Study. Reviewed International journal

    Kohei Fujimori, Toshiki Yoneda, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Yoshio Sugiura, Manabu Morita

    International journal of environmental research and public health   18 ( 15 )   2021.7

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    The aim of this two-year cohort study was to investigate salivary microRNAs (miRNAs) that predict periodontitis progression. A total of 120 patients who underwent supportive periodontal therapy were recruited. Unstimulated whole saliva was collected at baseline. Two years later, 44 patients were followed up (median age, 67.1 years) and divided into two groups: progression group (n = 22), with one or more sites with clinical attachment level (CAL) progression (>3 mm compared with baseline) or tooth extraction due to periodontitis progression; and the control group (n = 22), which did not exhibit CAL progression. In the microarray analysis of salivary miRNAs, hsa-miR-5571-5p, hsa-miR-17-3p, hsa-let-7f-5p, hsa-miR-4724-3p, hsa-miR-99a-5p, hsa-miR-200a-3p, hsa-miR-28-5p, hsa-miR-320d, and hsa-miR-31-5p showed fold change values <0.5 or ≥2.0 in the progression group compared with the control group (p < 0.05). On receiver operating characteristic curve analysis, areas under the curves of hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were >0.7, indicating fair discrimination power. The expressions of salivary hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were associated with periodontitis progression in patients with chronic periodontitis. These salivary miRNAs may be new biomarkers for progression of periodontitis, and monitoring them may contribute to new diagnostics and precision medicine for periodontitis.

    DOI: 10.3390/ijerph18158010

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  • Reasons for Tooth Extractions in Japan: The Second Nationwide Survey. Reviewed International journal

    Seitaro Suzuki, Naoki Sugihara, Hideyuki Kamijo, Manabu Morita, Takayuki Kawato, Midori Tsuneishi, Keita Kobayashi, Yoshihiro Hasuike, Tamotsu Sato

    International dental journal   2021.6

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    BACKGROUND: More than 10 years have passed since the first nationwide study on the reasons for tooth extraction in Japan. In the present study, we conducted the second nationwide survey to update the previous data. METHODS: This was a descriptive study. A sample population consisting of 5,250 dentists was selected by systematic random sampling using the 2018 membership directory of the Japan Dental Association. The reason for each permanent tooth extraction was documented by each dentist during a period of 1 week from June 4 to June 10, 2018. A questionnaire was provided for documentation. Reasons for tooth extraction were categorised into 6 groups as follows: caries, periodontal disease, fracture, orthodontics, impacted teeth, and others. RESULTS: A total of 2345 identified dentists responded to the questionnaire (recovery rate: 44.8%). Information on 7809 extracted teeth from 6398 patients was obtained. Periodontal disease was the main reason for tooth extraction for both sexes (men: 40.4%, women: 34.9%). Caries accounted for 30.2% of tooth extractions among men and 29.0% among women. Periodontal disease was predominant in the groups older than 55 years of age. Dental fracture accounted for 16.8% of tooth extractions among men and 19.2% among women. CONCLUSIONS: Caries and periodontal disease are still the main reasons for tooth extraction in Japan. Moreover, dentists should note that fractures accounted for approximately one-fifth of permanent tooth extractions after the age of 45 years.

    DOI: 10.1016/j.identj.2021.05.008

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  • Comprehensive Analysis of Risk Factors for Periodontitis Focusing on the Saliva Microbiome and Polymorphism. Reviewed International journal

    Naoki Toyama, Daisuke Ekuni, Daisuke Matsui, Teruhide Koyama, Masahiro Nakatochi, Yukihide Momozawa, Michiaki Kubo, Manabu Morita

    International journal of environmental research and public health   18 ( 12 )   2021.6

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    Few studies have exhaustively assessed relationships among polymorphisms, the microbiome, and periodontitis. The objective of the present study was to assess associations simultaneously among polymorphisms, the microbiome, and periodontitis. We used propensity score matching with a 1:1 ratio to select subjects, and then 22 individuals (mean age ± standard deviation, 60.7 ± 9.9 years) were analyzed. After saliva collection, V3-4 regions of the 16S rRNA gene were sequenced to investigate microbiome composition, alpha diversity (Shannon index, Simpson index, Chao1, and abundance-based coverage estimator) and beta diversity using principal coordinate analysis (PCoA) based on weighted and unweighted UniFrac distances. A total of 51 single-nucleotide polymorphisms (SNPs) related to periodontitis were identified. The frequencies of SNPs were collected from Genome-Wide Association Study data. The PCoA of unweighted UniFrac distance showed a significant difference between periodontitis and control groups (p < 0.05). There were no significant differences in alpha diversity and PCoA of weighted UniFrac distance (p > 0.05). Two families (Lactobacillaceae and Desulfobulbaceae) and one species (Porphyromonas gingivalis) were observed only in the periodontitis group. No SNPs showed significant expression. These results suggest that periodontitis was related to the presence of P. gingivalis and the families Lactobacillaceae and Desulfobulbaceae but not SNPs.

    DOI: 10.3390/ijerph18126430

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  • Parafunctional masseter muscle activity during waking is related to periodontitis progression: A pilot prospective cohort study. Reviewed International journal

    Daisuke Ekuni, Seiya Kato, Shigehisa Kawakami, Takayuki Maruyama, Kota Kataoka, Yoko Uchida-Fukuhara, Daiki Fukuhara, Naoki Toyama, Aya Yokoi, Md Monirul Islam, Subrina Binta Khair, Naoki Kodama, Manabu Morita

    Journal of clinical periodontology   48 ( 6 )   785 - 794   2021.6

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    AIM: The purpose of this pilot prospective cohort study was to investigate the effects of parafunctional masseter muscle activity on periodontitis progression among patients receiving supporting periodontal therapy (SPT). MATERIALS AND METHODS: We collected data of patients treated at Okayama University Hospital from August 2014 to September 2018. The progression group was defined as patients with ≥2 teeth demonstrating a longitudinal loss of proximal attachment of ≥3 mm during the 3-year study period and/or at least one tooth extraction due to periodontitis progression. Surface electromyography of masseter muscles at baseline was continuously recorded while patients were awake and asleep. RESULTS: We analysed 48 patients (36 females) aged 66.8 ± 9.1 years (mean ± SD). The rate of parafunctional masseter muscle activity during waking hours and sleeping hours at baseline was 60.4% and 52.1%, respectively. Cox's proportional hazards regression model showed that the incidence of periodontitis progression was significantly associated with number of teeth present (p = 0.001) and parafunctional masseter muscle activity during waking hours (p = 0.041). CONCLUSION: Our results suggest that parafunctional masseter muscle activity during waking hours is a risk factor for periodontitis progression among patients receiving SPT.

    DOI: 10.1111/jcpe.13432

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  • 「口臭による日常生活への支障」に関連する要因

    佐保 輝, 横井 彩, 丸山 貴之, 竹内 倫子, 江國 大輔, 森田 学

    日本口臭学会会誌   12 ( 1 )   11 - 17   2021.5

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    本調査の目的は、自己記入式質問票の「社会生活への支障」「家庭生活への支障」「行動制限」「自身の口臭が原因による他人の嫌がる態度やジェスチャー」の4項目を「口臭による日常生活への支障」と定義しその実態を把握すること、および、「口臭による日常生活への支障」と関連する要因について検討することである。2018年1月から2020年1月までの間に岡山大学病院口臭専門外来を受診した全患者32名(男性12名、女性20名、平均年齢53.2±16.2歳)を対象とした。オーラルクロマ(NISSHAエフアイエス株式会社、大阪)を用い、口腔内の揮発性硫黄化合物の濃度を測定した。自己記入式の質問票を用いて、上記4項目のほかに年齢、性別、口臭を意識するようになったきっかけ、口臭を意識した時期、頻回の歯磨き・うがい薬の使用・舌磨きによる口臭対策、医療者への相談経験を調査した。なお、口臭による日常生活への支障については、「社会生活への支障」「家庭生活への支障」「行動制限」「自身の口臭が原因による他人の嫌がる態度やジェスチャー」の各質問に対する回答【はい(1点)/いいえ(0点)】をもとに、合計スコア0〜4点で評価した。各質問項目との関連についてSpearmanの順位相関係数とカイ二重検定を用いて検討した。有意水準は5%とした。その結果、本調査で定義した「口臭による日常生活への支障」を有する患者が87.5%存在した。その支障の程度が大きい者は、長期にわたって口臭を意識しており、医療者への相談や複数の口臭対策を行っていた。一方で、実際の口腔内の揮発性硫黄化合物の濃度は有意に低いことが明らかになった。本調査結果は、口臭専門外来における新たな視点を提供する可能性がある。(著者抄録)

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  • Association between the incidence of early childhood caries and attending childcare among toddlers in a rural area of Japan: a prospective cohort study. Reviewed International journal

    Aya Yokoi, Noriko Takeuchi, Daisuke Ekuni, Manabu Morita

    Acta odontologica Scandinavica   79 ( 2 )   118 - 123   2021.3

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    OBJECTIVE: The purpose of this study was to investigate the association between the incidence of early childhood caries (ECC) and factors of the daytime childcare environment among toddlers in a rural area of Japan. MATERIAL AND METHODS: This was a prospective cohort study. Data collected from 2011 to 2015 were obtained for initially caries-free, 18-month-old toddlers (N = 640). Dentists recorded the numbers of decayed, missing and filled teeth (caries incidence) of toddlers at baseline (age 18 months) and follow-up (age 3 years). In addition, a questionnaire survey was conducted on guardians at baseline to record data on lifestyle. RESULTS: In total, 159 (24.8%) toddlers had newly identified ECC at follow-up. Logistic regression analyses indicated that the incidence of ECC was significantly associated with receiving daytime care at a nursery school (odds ratio [OR], 1.55; 95% confidence interval [CI], 1.05-2.30; p = .029), prolonged breastfeeding (OR, 1.71; 95%CI, 1.15-2.55; p = .008), and snacking ≥3 times a day (OR, 2.39; 95%CI, 1.53-3.74; p < .001). CONCLUSION: These findings suggest that receiving daytime care at a nursery school, prolonged breastfeeding, and frequent snacking are associated with an increased risk of ECC among Japanese toddlers in rural areas.

    DOI: 10.1080/00016357.2020.1795249

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  • [Current status and issues of community dental and oral health: Public Health Monitoring and Report Committee Oral Health Field Activity Summary].

    Hiroko Miura, Hideki Fukuda, Kazuo Kato, Noriko Takeuchi, Isao Watanabe, Miki Ojima, Koji Kamibayashi, Tomiko Shibata, Rumi Tano, Yoh Tamaki, Yuriko Hashimoto, Takashi Hanioka, Masumi Muramatsu, Yoshiko Motohashi, Manabu Morita, Tetsunori Ozaki

    [Nihon koshu eisei zasshi] Japanese journal of public health   68 ( 2 )   83 - 91   2021.2

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    DOI: 10.11236/jph.20-106

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  • Oral diadochokinesis is related to decline in swallowing function among community-dwelling Japanese elderly: a cross-sectional study. Reviewed International journal

    Noriko Takeuchi, Nanami Sawada, Daisuke Ekuni, Manabu Morita

    Aging clinical and experimental research   33 ( 2 )   399 - 405   2021.2

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    BACKGROUND: Since oral organs function in the first stage of the swallowing process, it is possible that decline in swallowing function can be partly related to oral frailty. AIMS: The purpose of this study was to investigate the association between swallowing function and oral function among community-dwelling elderly Japanese and to propose cut-off values for screening of decline in swallowing function. METHODS: A total of 188 participants were included in the analysis. The number of present teeth and functioning teeth were counted. Bacteria counts in tongue coat, oral wettability, tongue pressure, oral diadochokinesis (ODK), masticatory ability and bite force were examined. Swallowing function was assessed using the 10-item Eating Assessment Tool (EAT-10). Receiver operating characteristic curves were used to determine the cut-off points of each oral function parameter. A logistic regression model was performed to determine the combination of parameters with the highest accuracy for differentiating decline in swallowing function from normal. RESULTS: Subjects with ODK /pa/ sound < 6.2 times/second had higher prevalence of swallowing problems than those with 6.2 times/second or more. EAT-10 scores of one or more were significantly related to older age (≥ 71 years old; odds ratio [OR] 4.321) and reduced ODK /pa/ sound (< 6.2 times/second; OR 2.914). CONCLUSIONS: Among community-dwelling elderly Japanese, those who were suspected of having decline in swallowing function had lower oral function than those who did not, and the cut-off values were 71 years of age and ODK /pa/ sound 6.2 times/s.

    DOI: 10.1007/s40520-020-01547-7

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  • Living with Family Is Directly Associated with Regular Dental Checkup and Indirectly Associated with Gingival Status among Japanese University Students: A 3-Year Cohort Study. Reviewed International journal

    Momoko Nakahara, Daisuke Ekuni, Kota Kataoka, Aya Yokoi, Yoko Uchida-Fukuhara, Daiki Fukuhara, Terumasa Kobayashi, Naoki Toyama, Hikari Saho, Md Monirul Islam, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   18 ( 1 )   2021.1

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    Although some studies showed that lifestyle was associated with oral health behavior, few studies investigated the association between household type and oral health behavior. The aim of this prospective cohort study was to investigate the association between household type, oral health behavior, and periodontal status among Japanese university students. Data were obtained from 377 students who received oral examinations and self-questionnaires in 2016 and 2019. We assessed periodontal status using the percentage of bleeding on probing (%BOP), probing pocket depth, oral hygiene status, oral health behaviors, and related factors. We used structural equation modeling to determine the association between household type, oral health behaviors, gingivitis, and periodontitis. At follow-up, 252 students did not live with their families. The mean ± standard deviation of %BOP was 35.5 ± 24.7 at baseline and 32.1 ± 25.3 at follow-up. In the final model, students living with their families were significantly more likely to receive regular dental checkup than those living alone. Regular checkup affected the decrease in calculus. The decrease in calculus affected the decrease in %BOP over 3 years. Living with family was directly associated with regular dental checkups and indirectly contributed to gingival status among Japanese university students.

    DOI: 10.3390/ijerph18010324

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  • Association of dental occlusal support with the Prognostic Nutritional Index in patients with esophageal cancer who underwent esophagectomy. Reviewed

    Reiko Yamanaka-Kohno, Yasuhiro Shirakawa, Mami Inoue-Minakuchi, Aya Yokoi, Misato Muro, Hirotaka Kosaki, Shunsuke Tanabe, Toshiyoshi Fujiwara, Manabu Morita

    Esophagus : official journal of the Japan Esophageal Society   18 ( 1 )   49 - 55   2021.1

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    BACKGROUND: The Prognostic Nutritional Index is useful for predicting surgical risk and overall survival based on preoperative immunological and nutritional status in patients undergoing digestive organ cancer surgery. The purpose of this study was to examine the association between the Prognostic Nutritional Index and dental status in patients with esophageal cancer who underwent esophagectomy. METHODS: This retrospective case-control study included 73 patients who underwent resection of esophageal cancer (69 males, 4 females; age 36-83). General and dental status were evaluated. The Prognostic Nutritional Index was calculated based on the serum albumin concentration and the total lymphocyte count, and subjects were divided into two groups based on index scores: a higher group, characterized by scores ≥ 45 (n = 54); and a lower group, characterized by scores < 45 (n = 19). Univariate analysis and multiple logistic regression analyses were used to compare between groups. RESULTS: Total protein, C-reactive protein, the number of sound and total decayed, missing and filled teeth, and the rate of patients with poor dental occlusal support showed significant differences between the lower and higher Prognostic Nutritional Index groups (p < 0.05). Stepwise logistic regression analysis by backward selection approach showed that low total protein, few sound teeth, and poor status of dental occlusal support were significantly associated with the lower Prognostic Nutritional Index (p = 0.007, 0.042, and 0.009, respectively). CONCLUSION: Dental status, especially dental occlusal support and the number of sound teeth, showed a positive relationship with the Prognostic Nutritional Index in esophageal cancer patients who underwent esophagectomy.

    DOI: 10.1007/s10388-020-00751-8

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  • A multicenter prospective cohort study on the effect of smoking cessation on periodontal therapies in Japan. Reviewed

    Yohei Nakayama, Koji Mizutani, Yuka Tsumanuma, Hiroyuki Yoshino, Norio Aoyama, Koji Inagaki, Manabu Morita, Yuichi Izumi, Shinya Murakami, Hidenori Yoshimura, Takanori Matsuura, Takashi Murakami, Matsuo Yamamoto, Nobuo Yoshinari, Masaru Mezawa, Yorimasa Ogata, Atsutoshi Yoshimura, Kanji Kono, Kosuke Maruyama, Soh Sato, Ryuji Sakagami, Hiroshi Ito, Yukihiro Numabe, Masahiko Nikaido, Takashi Hanioka, Kanichi Seto, Jinichi Fukuda, Saman Warnakulasuriya, Toru Nagao

    Journal of oral science   63 ( 1 )   114 - 118   2020.12

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    Few prospective studies have reported the effects of periodontal therapy on patients who attempted to quit smoking. This study aimed to assess how smoking cessation affects periodontal therapy. Twenty-five smokers with periodontitis were investigated by dividing them into two groups, a smoking cessation support group and a continued smoking group. Those in the support group received counseling and nicotine replacement therapy, followed by periodontal treatment conducted by dentists who had completed an e-learning course on smoking cessation. Clinical parameters were measured at baseline, 3, and 6 months. Most clinical parameters improved for those in the smoking cessation support group. There were no significant improvements in bleeding on probing (BOP) or the number of severe periodontal disease sites in the continued smoking group. Probing pocket depth (PPD) and clinical attachment levels (CAL) at sites that received scaling and root planing (SRP) significantly improved in all subjects. BOP did not improve at reevaluation in the smoking relapse subgroup. Patients in the smoking cessation support program led by dental professionals showed more improvement in BOP than those in the continued smoking group.

    DOI: 10.2334/josnusd.20-0288

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  • Association between Household Exposure to Secondhand Smoke and Dental Caries among Japanese Young Adults: A Cross-Sectional Study. Reviewed International journal

    Hikari Saho, Ayano Taniguchi-Tabata, Daisuke Ekuni, Aya Yokoi, Kouta Kataoka, Daiki Fukuhara, Naoki Toyama, Md Monirul Islam, Nanami Sawada, Yukiho Nakashima, Momoko Nakahara, Junya Deguchi, Yoko Uchida-Fukuhara, Toshiki Yoneda, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   17 ( 22 )   2020.11

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    The long-term effects of secondhand smoke (SHS) on dental caries among Japanese young adults remain unclear. The purpose of this cross-sectional study was to evaluate whether household exposure to SHS is associated with dental caries in permanent dentition among Japanese young adults. The study sample included 1905 first-year university students (age range: 18-19 years) who answered a questionnaire and participated in oral examinations. The degree of household exposure to SHS was categorized into four levels according to the SHS duration: no experience (-), past, current SHS < 10 years, and current SHS ≥ 10 years. Dental caries are expressed as the total number of decayed, missing, and filled teeth (DMFT) score. The relationships between SHS and dental caries were determined by logistic regression analysis. DMFT scores (median (25th percentile, 75th percentile)) were significantly higher in the current SHS ≥ 10 years (median: 1.0 (0.0, 3.0)) than in the SHS-(median: 0.0 (0.0, 2.0)); p = 0.001). DMFT ≥ 1 was significantly associated with SHS ≥ 10 years (adjusted odds ratio: 1.50, 95% confidence interval: 1.20-1.87, p < 0.001). Long-term exposure to SHS (≥10 years) was associated with dental caries in permanent dentition among Japanese young adults.

    DOI: 10.3390/ijerph17228623

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  • 福祉施設利用高齢者における日常生活自立度と口腔機能との関係 Reviewed

    中島 千穂, 竹内 倫子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   70 ( 4 )   215 - 221   2020.10

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    日常生活動作ADLと現在歯数、咀嚼能力、オーラルディアドコキネシス(ODK)との関連が報告されているが、一定の見解を得られていない。また、過去の研究では単一施設内での報告が多く、複数施設での報告は少ない。本研究の目的は多施設で口腔機能とADLとの関連を調べることであった。岡山県北部の6ヶ所の福祉施設を利用した高齢者61名を対象とし、年齢、性別、要介護度、基礎疾患、ADL、食事形態、現在歯数、義歯の使用の有無、舌圧、ODKを調査した。食形態は「常食」と「常食以外」の2群に分類し、ODKは「/pa/」「/ta/」「/ka/」それぞれを「4.0回/秒未満」「4.0回/秒以上」の2群に分類した。ADLはランクJを「生活自立群」、ランクAを「準寝たきり群」、ランクBおよびCを「寝たきり群」と3群に分類した。ADLと年齢の関連はKruskal-Wallis検定を用い、ADLと年齢以外の各項目、ODKと食形態の関連はFisherの正確確率検定を用いて分析した。その結果、ADLの重症度は要介護度、糖尿病、骨関節疾患、食形態に加え、ODK「/pa/」「/ka/」との間に有意な関連がみられた。また、ODKすべての音節と食形態との間に有意な関連がみられた。ODKの低下が食形態の変化を介して栄養状態の悪化を招き、ADLの重症度に関連した可能性が示された。(著者抄録)

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  • 高校生におけるDMF歯数の増加と口腔保健行動との関連 Reviewed

    澤田 ななみ, 竹内 倫子, 田畑 綾乃, 江國 大輔, 森田 学

    口腔衛生学会雑誌   70 ( 4 )   190 - 195   2020.10

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    これまで幼児や小・中学生のう蝕発症の要因に関する報告は多いが、高校生では少なく、縦断研究はほとんどない。本研究では高校生のう蝕発症に関連する要因を調べることを目的に、2年間の前向きコホート研究を行った。対象者は岡山市内の私立高等学校で、2016年4月(1年生)(ベースライン)と2018年4月(3年生)(再評価)に定期健康診断で歯・口腔の健康診断を受診した生徒とした。口腔内診査と質問票調査を行い、2年間のDMF歯数増加とベースライン時の口腔保健行動との関連をロジスティック回帰分析で検討した。160名(男子62名、女子98名)(平均年齢15.0±0.24歳)を分析した。ベースライン時のDMF歯数は0.86±1.75であった。DMF歯数増加群は72名(45.0%)であった。ロジスティック回帰分析の結果から、DMF歯数増加と有意な関連が認められたのは、「1年生時のDMF歯数が1以上」「フッ素入り歯磨剤を使用しない」「糖質ゼロ等表示食品を選ぶ」および「過去1年間に歯科医院で歯のそうじを受けた」の4項目であった(p<0.05)。結論として、高校生において、フッ素入り歯磨剤を使用することがう蝕発症を抑制する可能性が示唆された。(著者抄録)

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  • Horseradish peroxidase interacts with the cell wall peptidoglycans on oral bacteria. Reviewed International journal

    Hirofumi Mizuno, Eiji Takayama, Ayano Satoh, Takeshi Into, Masanori Adachi, Daisuke Ekuni, Koji Yashiro, Masako Mizuno-Kamiya, Motohiko Nagayama, Seitaro Saku, Takaaki Tomofuji, Yutaka Doi, Yukitaka Murakami, Nobuo Kondoh, Manabu Morita

    Experimental and therapeutic medicine   20 ( 3 )   2822 - 2827   2020.9

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    Salivary peroxidase and myeloperoxidase are known to display antibacterial activity against oral microbes, and previous indications have pointed to the possibility that horseradish peroxidase (HRP) adsorbs onto the membrane of the major oral streptococci, Streptococcus mutans and Streptococcus sanguinis (S. sanguinis). However, the mechanism of interaction between HRP and the bacterial cell wall component is unclear. Dental plaques containing salivary glycoproteins and extracellular microbial products are visualized with 'dental plaque disclosing agent', and are controlled within dental therapy. However, current 'dental plaque disclosing agents' are difficult to evaluate with just dental plaques, since they stain and disclose not only dental plaques but also pellicle formed with salivary glycoproteins on a tooth surface. In this present study, we have demonstrated that HRP interacted with the cell wall component of the major gram-positive bacterial peptidoglycan, but not the major cell wall component of gram-negative bacteria lipopolysaccharide. Furthermore, we observed that the adsorbed HRP labeled with fluorescence was detected on the major oral gram-positive strains S. sanguinis and Streptococcus salivarius (S. salivarius), but not on a gram-negative strain, Escherichia coli (E. coli). Furthermore, we have demonstrated that the combination of HRP and chromogenic substrate clearly disclosed the dental plaques and the biofilm developed by S. sanguinis, S. salivarius and the major gram-postive bacteria Lactobacillus casei on tooth surfaces, and slightly disclosed the biofilm by E. coli. The combination of HRP and chromogenic substrate did not stain either the dental pellicle with the salivary glycoprotein mucin, or naked tooth surfaces. These results have suggested the possibility that the adsorption activity of HRP not only contributes to the evaluation of dental plaque, but that enzymatic activity of HRP may also contribute to improve dental hygiene.

    DOI: 10.3892/etm.2020.9016

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  • 抗菌的光線力学療法が歯肉縁下細菌叢の形成に与える影響について Reviewed

    入江 浩一郎, 田畑 綾乃, 内田 瑶子, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   70 ( 3 )   144 - 151   2020.7

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    非侵襲的な歯周病の治療法として抗菌的光線力学療法(antimicrobial photodynamic therapy;a-PDT)が注目されている。a-PDTの臨床効果を評価した報告は多数あるが、細菌叢に着目した研究は少ない。本研究では、歯肉炎を有する学生ボランティアを対象に、a-PDTによる歯肉縁下細菌叢の形成に与える影響について検討した。下顎第一、第二大臼歯部に歯肉炎を有する男子学生6名(平均年齢24.2±0.98歳)を対象にした。被験者の下顎第一、第二大臼歯部を左右で分割し、照射(a-PDT)群もしくは対照群をコイントスで割付けた。照射群ではスケーリングとa-PDTを、そして対照群ではスケーリングを行った。処置開始前と処置の2週間後に、下顎第一、第二大臼歯部歯間部歯肉縁下から細菌を採取した。サンプルからDNAを抽出し、次世代シーケンス解析を行った。その結果、Red complexに属する細菌の割合は、照射群において処置前と処置後との間で有意な減少を認めた(p<0.05)。一方、対照群においては、処置前後で有意差は認められなかった。本研究から、スケーリングとa-PDTを併用した場合、歯肉縁下細菌叢中の歯周病原性の高い細菌の占める割合が有意に減少することが示された。(著者抄録)

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  • Caries Increment and Salivary Microbiome during University Life: A Prospective Cohort Study. Reviewed International journal

    Yoko Uchida-Fukuhara, Daisuke Ekuni, Md Monirul Islam, Kota Kataoka, Ayano Taniguchi-Tabata, Daiki Fukuhara, Naoki Toyama, Terumasa Kobayashi, Kohei Fujimori, Nanami Sawada, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   17 ( 10 )   2020.5

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    The purpose of this 3-year prospective cohort study was to explore the relationship between an increase in dental caries and oral microbiome among Japanese university students. We analyzed 487 students who volunteered to receive oral examinations and answer baseline (2013) and follow-up (2016) questionnaires. Of these students, salivary samples were randomly collected from 55 students at follow-up and analyzed using next-generation sequencing. Students were divided into two groups: increased group (Δdecayed, missing, and filled teeth (ΔDMFT) score increased during the 3-year period) and non-increased group (ΔDMFT did not increase). Thirteen phyla, 21 classes, 32 orders, 48 families, 72 genera, and 156 species were identified. Microbial diversity in the increased group (n = 14) was similar to that in the non-increased group (n = 41). Relative abundances of the family Prevotellaceae (p = 0.007) and genera Alloprevotella (p = 0.007) and Dialister (p = 0.039) were enriched in the increased group compared with the non-increased group. Some bacterial taxonomic clades were differentially present between the two groups. These results may contribute to the development of new dental caries prevention strategies, including the development of detection kits and enlightenment activities for these bacteria.

    DOI: 10.3390/ijerph17103713

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  • Association between Sleep Quality and Duration and Periodontal Disease among University Students: A Cross-Sectional Study Reviewed

    Md Monirul Islam, Daisuke Ekuni, Naoki Toyama, Ayano Taniguchi-Tabata, Kota Kataoka, Yoko Uchida-Fukuhara, Daiki Fukuhara, Hikari Saho, Nanami Sawada, Yukiho Nakashima, Yoshiaki Iwasaki, Manabu Morita

    International Journal of Environmental Research and Public Health   17 ( 9 )   3034 - 3034   2020.4

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    The purpose of this cross-sectional study was to investigate the association between sleep quality and duration, and periodontal disease among a group of young Japanese university students. First-year students (n = 1934) at Okayama University who voluntarily underwent oral health examinations were included in the analysis. Sleep quality and duration were assessed by the Japanese version of the Pittsburgh Sleep Quality Index. Dentists examined Oral Hygiene Index-Simplified (OHI-S), probing pocket depth (PPD), and percentage of sites with bleeding on probing (BOP). Periodontal disease was defined as presence of PPD ≥ 4 mm and BOP ≥ 30%. Overall, 283 (14.6%) students had periodontal disease. Poor sleep quality was observed among 372 (19.2%) students. Mean (± standard deviation) sleep duration was 7.1 ± 1.1 (hours/night). In the logistic regression analysis, periodontal disease was significantly associated with OHI-S (odds ratio [OR]: 2.30, 95% confident interval [CI]: 1.83–2.90; p &lt; 0.001), but not sleep quality (OR: 1.09, 95% CI: 0.79–1.53; p = 0.577) or sleep duration (OR: 0.98, CI: 0.87–1.10; p = 0.717). In conclusion, sleep quality and duration were not associated with periodontal disease among this group of young Japanese university students.

    DOI: 10.3390/ijerph17093034

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  • 歯周病安定期治療中の患者における二酸化塩素溶液洗口の効果 ランダム化二重盲検プラセボ対照比較試験

    佐保 輝, 横田 麻美, 備中 香月, 本田 俊一, 森田 学

    日本口臭学会会誌   11 ( 1 )   17 - 25   2020.4

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    二酸化塩素を有効成分とする洗口剤の口臭に対する有用性は多く報告されているが、歯周病に対する効果を評価した研究は乏しい。本研究の目的は歯周病安定期治療中の患者における二酸化塩素を含む洗口剤の効果を検討することである。歯周病安定期治療中の患者42名を2群に分け、ランダム化二重盲検プラセボ対照比較試験を行った。被験者は二酸化塩素を含む洗口剤またはプラセボ洗口剤で1日2回、3ヵ月間洗口するように指示された。観察開始時(ベースライン)、1ヵ月後、3ヵ月後にPlaque Control Record(PCR)、プロービング時出血、歯周ポケット深さを評価した。統計分析は、2群間の比較にはカイ二乗検定もしくはMann-Whitney U検定を用い、群内比較にはWilcoxon符号付順位和検定を用いた。群間比較において、二酸化塩素の有無は歯周状態に影響しなかった。しかしながら、二酸化塩素を含む洗口剤を使用した群では、PCRの有意な減少がみられた。今後は歯周病重症度の異なる患者を対象とし、ブラッシング回数や清掃補助器具の使用などの背景因子を揃えた更なる研究が必要である。(著者抄録)

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  • Associations between sleep bruxism, sleep quality, and exposure to secondhand smoke in Japanese young adults: a cross-sectional study. Reviewed International journal

    Naoki Toyama, Daisuke Ekuni, Ayano Taniguchi-Tabata, Toshiki Yoneda, Kota Kataoka, Aya Yokoi, Yoko Uchida, Daiki Fukuhara, Hikari Saho, Islam Md Monirul, Nanami Sawada, Yukiho Nakashima, Yoshiaki Iwasaki, Manabu Morita

    Sleep medicine   68   57 - 62   2020.4

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    OBJECTIVE: Sleep bruxism, a major sleep disorder that causes serious harm to oral health, is considered a multifactorial disease. Sleep bruxism can be induced by smoking, which also adversely affects sleep quality. The objective of present study was to clarify the associations between sleep bruxism, sleep quality, and exposure to secondhand smoke (SHS). METHODS: To assess the prevalence of sleep bruxism, sleep quality, and SHS exposure, we conducted oral examinations and self-report questionnaires on university students in Japan. Sleep bruxism and quality were screened using the Japanese version of the Pittsburgh Sleep Quality Index (PSQI) and the third edition of the International Classification of Sleep Disorders (ICSD-3). The inclusion criteria were adults aged between 18 and 19 years, non-smokers and non-alcohol drinkers. The exclusion criteria was failing to complete the questionnaire in full. RESULTS: We analyzed a total of 1781 Japanese young adults. Young adult females who had been exposed to SHS had worse sleep quality (p = 0.019) than those who had not. Young adult female with worse sleep quality showed a higher prevalence of sleep bruxism (p = 0.034) than those with better sleep quality. Using structural equation modeling, direct associations were identified between SHS exposure and poor sleep quality (standardized coefficients, 0.153; p = 0.008) and between sleep bruxism and poor sleep quality (standardized coefficients, 0.187; p = 0.022) in young adult females. However, no association was found among young adult males. CONCLUSION: SHS exposure is indirectly associated with sleep bruxism through poor sleep quality in Japanese young adult females.

    DOI: 10.1016/j.sleep.2019.09.003

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  • Relationship of Salivary Microbiome with the Worsening of the Periodontal Health Status in Young Adults: A 3-Year Cohort Study. Reviewed International journal

    Md Monirul Islam, Daisuke Ekuni, Naoki Toyama, Terumasa Kobayashi, Kohei Fujimori, Yoko Uchida, Daiki Fukuhara, Ayano Taniguchi-Tabata, Kota Kataoka, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   17 ( 5 )   2020.3

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    The purpose of this prospective cohort study was to investigate the influence of the salivary microbiome on the worsening of the periodontal health status among Japanese young adults. We assessed the data of systemically healthy and non-smoking young (18-22 years) university students (n = 457) from Okayama University at baseline (2013) and follow-up (2016). The worsening group was defined based on an increase in the percentage of bleeding on probing (%BOP) or an increase in probing pocket depth (PPD) from <4 mm to ≥4 mm. Unstimulated saliva samples were randomly collected from 69 students for microbiome analysis at follow-up. The salivary microbiome was assessed through 16S rRNA metagenomic sequencing. The type of community in the salivary microbiome clustered by statistical analysis and diversity was not significantly associated with the worsening of the periodontal health status in cases of increasing %BOP and PPD (p > 0.05). The prevalence of some species was significantly higher in the worsening group than in the non-worsening group (p < 0.05) in both cases. The worsening of the periodontal health status was associated with some species, but not the type of community and diversity in the salivary microbiome among Japanese young adults.

    DOI: 10.3390/ijerph17051764

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  • Association between intensive health guidance focusing on eating quickly and metabolic syndrome in Japanese middle-aged citizens. Reviewed International journal

    Daisuke Ekuni, Michiko Furuta, Toshihide Kimura, Naoki Toyama, Daiki Fukuhara, Yoko Uchida, Nanami Sawada, Manabu Morita

    Eating and weight disorders : EWD   25 ( 1 )   91 - 98   2020.2

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    PURPOSE: The purpose of this intervention study was to investigate whether intensive health guidance focusing on eating quickly can prevent metabolic syndrome (MetS) more effectively than standard routine guidance in Japanese citizens living in rural areas. METHODS: This controlled, non-randomized, intervention study analyzed 141 participants with MetS at baseline. Participants in the intervention group received health guidance focusing on eating quickly and standard health guidance about MetS in accordance with the guidelines of the Ministry of Health, Labour and Welfare in Japan, whereas participants in the control group received only standard health guidance about MetS. The primary study outcome was the prevalence of MetS at a 1-year follow-up. RESULTS: At 1-year follow-up, the prevalence of MetS in the intervention group was significantly lower than that in the control group (p = 0.003). The decreases in body weight, body mass index, waist circumference and triglycerides from baseline to 1 year were significantly greater in the intervention group than in the control group (p < 0.05). CONCLUSION: Intensive health guidance focusing on eating quickly is more effective for improving MetS than standard Japanese health guidance alone. LEVEL OF EVIDENCE: Level II, Evidence obtained from well-designed controlled trials without randomization. TRIAL REGISTRY NAME, REGISTRATION IDENTIFICATION NUMBER, AND URL FOR THE REGISTRY: UMIN, UMIN000030600, http://www.umin.ac.jp/ctr/index-j.htm.

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  • 永久歯の主原因別抜歯状況の推計に関する研究 第2回永久歯の抜歯原因調査、NDBオープンデータによる Reviewed

    上條 英之, 森田 学, 川戸 貴行, 恒石 美登里, 小林 慶太, 高野 直久, 蓮池 芳浩, 佐藤 保

    日本歯科医療管理学会雑誌   54 ( 4 )   268 - 274   2020.2

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    わが国の年間抜歯本数と抜歯治療を受けた患者数(抜歯者数)について、主原因別の状況を推計することを目的とした。第2回抜歯原因調査(日本歯科医師会および8020推進財団、2018年)、第4回NDBオープンデータ(厚生労働省保険局、2019年)およびわが国の人口推計(総務省、2018年)を用いて1年間の抜歯状況を推計した。その結果、2017年度の人口千人対抜歯者数は80人であった。抜歯の主原因別にみた人口千人対抜歯者数は、歯周病30人、う蝕23人、破折14人の順であり、抜歯本数についても同様の順序を示した。年齢階級別に抜歯者数をみると、歯周病と破折はともに増齢とともに増え続け、75〜79歳でピークを示した。また、う蝕による抜歯は、25〜29歳と80〜84歳で2つのピークが示された。(著者抄録)

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  • Detection of Serum miRNAs Affecting Liver Apoptosis in a Periodontitis Rat Model Reviewed

    YOSHIO SUGIURA, TOSHIKI YONEDA, KOHEI FUJIMORI, TAKAYUKI MARUYAMA, HISATAKA MIYAI, TERUMASA KOBAYASHI, DAISUKE EKUNI, TAKAAKI TOMOFUJI, MANABU MORITA

    In Vivo   34 ( 1 )   117 - 123   2020.2

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    DOI: 10.21873/invivo.11752

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  • Association between maternal periodontal status and ultrasonographic measurement of fetal growth: A longitudinal study. Reviewed International journal

    Ayano Taniguchi-Tabata, Noriko Takeuchi, Yoko Uchida, Daisuke Ekuni, Manabu Morita

    Scientific reports   10 ( 1 )   1402 - 1402   2020.1

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    The aim of this prospective cohort study was to investigate the association between intrauterine fetal growth patterns and periodontal status in pregnant women. Fifty-three pregnant women were recruited. Periodontitis was diagnosed based on probing pocket depth and clinical attachment level. Maternal urinary 8-hydroxy-2'-deoxyguanosine levels and body mass index were recorded. Ultrasonographic measurements of the biparietal diameter (BPD), abdominal circumference (AC), and femur length (FL) were recorded, and estimated fetal weight (EFW) was calculated. In addition, approximation spline curves of BPD, AC, FL, and EFW were obtained throughout the gestation period. Recorded delivery outcomes were gestational age (GA), birth weight and length, sex, placental weight, and umbilical cord length. Forty-four participants (34.1 ± 4.9 years) were analyzed. Mean neonatal birth weight was 2906.0 ± 544.4 g. On multiple regression analysis, birth weight was related with periodontitis (standardized β = -0.21, P = 0.001). For EFW and BPD, the curves of the periodontitis group were located lower than those of the non-periodontitis group, with significant differences after 32 weeks and 20 weeks of GA, respectively. In conclusion, periodontal treatment before conception may be recommended and a good periodontal condition in the early stage of pregnancy at the latest is desirable for infant growth.

    DOI: 10.1038/s41598-020-58396-7

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  • Effects of Coffee Intake on Oxidative Stress During Aging-related Alterations in Periodontal Tissue Reviewed

    TERUMASA KOBAYASHI, TAKAYUKI MARUYAMA, TOSHIKI YONEDA, HISATAKA MIYAI, TETSUJI AZUMA, TAKAAKI TOMOFUJI, DAISUKE EKUNI, MANABU MORITA

    In Vivo   34 ( 2 )   615 - 622   2020

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    DOI: 10.21873/invivo.11815

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  • Relationship between oral hygiene knowledge, source of oral hygiene knowledge and oral hygiene behavior in Japanese university students: A prospective cohort study. Reviewed International journal

    Daiki Fukuhara, Daisuke Ekuni, Kota Kataoka, Ayano Taniguchi-Tabata, Yoko Uchida-Fukuhara, Naoki Toyama, Toshiki Yoneda, Yoshio Sugiura, Md Monirul Islam, Hikari Saho, Yoshiaki Iwasaki, Manabu Morita

    PloS one   15 ( 7 )   e0236259   2020

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    The aim of this prospective cohort study was to examine whether oral hygiene knowledge, and the source of that knowledge, affect oral hygiene behavior in university students in Japan. An oral exam and questionnaire survey developed to evaluate oral hygiene knowledge, the source of that knowledge, and oral hygiene behavior, such as the frequency of tooth brushing and regular dental checkups and the use of dental floss, was conducted on university student volunteers. In total, 310 students with poor tooth brushing behavior (frequency of tooth brushing per day [≤ once]), 1,963 who did not use dental floss, and 1,882 who did not receive regular dental checkup during the past year were selected. Among these students, 50, 364, and 343 in each respective category were analyzed in over the 3-year study period (follow-up rates: 16.1%, 18.5%, and 18.2%, respectively). The odds ratios (ORs) and 95% confidence intervals (CIs) for oral hygiene behavior were calculated based on oral hygiene knowledge and the source of that knowledge using logistic regression models. The results showed that dental clinics were the most common (> 50%) source of oral hygiene knowledge, and that a more frequent use of dental floss was significantly associated with dental clinics being a source of oral hygiene knowledge (OR, 4.11; 95%CI, 1.871-9.029; p < 0.001). In addition, a significant association was seen between dental clinics being a source of oral hygiene knowledge and more frequent regular dental checkups (OR, 13.626; 95%CI, 5.971-31.095; p < 0.001). These findings suggest the existence of a relationship between dental clinics being the most common source of oral hygiene knowledge and improved oral hygiene behavior in Japanese university students.

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  • Structural equation modeling to detect predictors of oral health-related quality of life among Japanese university students: a prospective cohort study. Reviewed International journal

    Hikari Saho, Daisuke Ekuni, Kota Kataoka, Ayano Taniguchi-Tabata, Naoki Toyama, Yoshio Sugiura, Md Monirul Islam, Yoshiaki Iwasaki, Manabu Morita

    Quality of life research : an international journal of quality of life aspects of treatment, care and rehabilitation   28 ( 12 )   3213 - 3224   2019.12

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    PURPOSE: This prospective cohort study of Japanese university students aimed to identify factors that might affect oral health-related quality of life (OHRQoL) and longitudinal relationships over a period of 3 years. METHODS: Students (n = 487) provided complete data before entering and 3 years later (before university graduation) participated in the present study. Decayed, missing, and filled teeth (DMFT) scores, community periodontal index, ratios (%) of teeth with bleeding on probing, and malocclusion were determined during oral examinations. The questionnaire addressed age, sex, self-rated oral health, oral health behavior, subjective oral symptoms, and OHRQoL determined using the oral health impact profile (OHIP)-14. Associations were analyzed using structural equation modeling (SEM). RESULTS: The OHRQoL significantly worsened according to OHIP-14 total score (p = 0.001). The final model in the symptoms of SEM analysis showed that OHRQoL at follow-up positively correlated with OHRQoL at baseline. Self-rated oral health was directly associated with the OHRQoL at baseline (p < 0.05). CONCLUSIONS: This study determined that OHRQoL at baseline was a direct predictor, and that self-rated oral health were indirect predictors of OHRQoL at follow-up among Japanese university students.

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  • Quasi-Randomized Trial of Effects of Perioperative Oral Hygiene Instruction on Inpatients with Heart Diseases Using a Behavioral Six-Step Method. Reviewed International journal

    Chie Omori, Daisuke Ekuni, Yumiko Ohbayashi, Minoru Miyake, Manabu Morita

    International journal of environmental research and public health   16 ( 21 )   2019.11

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    The assessor-blinded, parallel-design, quasi-randomized study (alternating allocation) aimed to determine the effects of the six-step method on postoperative numbers of oral bacteria, periodontal status, and atrial fibrillation (AF) among inpatients with heart diseases and periodontitis. Seventy inpatients who received preoperative periodontal treatment were quasi-randomly assigned to intervention and control groups at University Hospital. The intervention group received intensive oral hygiene instruction using a six-step method for 15 minutes per week and the control group received routine oral hygiene instruction. Significantly fewer oral bacteria were identified on the tongue at discharge compared with baseline in the intervention than the control group (ANCOVA) (large effect size, p = 0.02). Changes in scores for self-efficacy, plaque scores, probed pocket depth, and bleeding on probing between baseline and discharge were significantly greater in the intervention, than in the control group (p < 0.05). The period of postoperative AF (days) was significantly shorter in the intervention, than in the control group (p = 0.019). In conclusion, oral hygiene instruction using the six-step method decreased the numbers of oral bacteria on the tongue and improved self-efficacy, oral health behaviors, oral hygiene status, periodontal status, and period of postoperative AF among inpatients with periodontitis and heart diseases.

    DOI: 10.3390/ijerph16214252

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  • Influence of Occupational Stress and Coping Style on Periodontitis among Japanese Workers: A Cross-Sectional Study. Reviewed International journal

    Md Monirul Islam, Daisuke Ekuni, Toshiki Yoneda, Aya Yokoi, Manabu Morita

    International journal of environmental research and public health   16 ( 19 )   2019.9

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    The aim of this cross-sectional study was to evaluate the association between the influence of occupational stress and coping style on periodontitis among Japanese workers. The study sample included 738 workers (age range: 19-65 years) at a manufacturing company in Kagawa Prefecture, Japan. To analyze occupational stress and coping style, all participants answered a self-report questionnaire composed of items on their work environment and oral health behavior. Oral examinations were performed by calibrated dentists. Among all workers, 492 (66.7%) workers were diagnosed with periodontitis, and 50 (6.8%) were diagnosed with a high stress-low coping condition. Significant differences (p < 0.05) were observed between the periodontitis and non-periodontitis groups in terms of age, gender, body mass index, smoking status, daily alcohol drinking, monthly overtime work, worker type, and stress-coping style. Logistic regression analysis showed that a high stress-low coping condition was associated with an increased risk of periodontitis (odds ratio: 2.79, 95% confidence interval: 1.05-7.43, p = 0.039). These findings suggest that a high stress-low coping condition is associated with periodontitis among the 19-65 years of age group of Japanese workers.

    DOI: 10.3390/ijerph16193540

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  • Incidence of the Acute Symptom of Chronic Periodontal Disease in Patients Undergoing Supportive Periodontal Therapy: A 5-Year Study Evaluating Climate Variables. Reviewed International journal

    Hikari Saho, Noriko Takeuchi, Daisuke Ekuni, Manabu Morita

    International journal of environmental research and public health   16 ( 17 )   2019.8

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    Although patients under supportive periodontal therapy (SPT) have a stable periodontal condition, the acute symptom of chronic periodontal disease occasionally occurs without a clear reason. Therefore, in the present study, to obtain a better understanding of this relationship in patients undergoing SPT, we hypothesized that the acute symptom of chronic periodontal disease might be affected by climate factors. We conducted a questionnaire study and carried out oral examinations on patients undergoing SPT who had been diagnosed as having the acute symptom of chronic periodontal disease. We collected climate data from the local climate office in Okayama city, Japan. We predicted parameters that affect the acute symptom of chronic periodontal disease with unidentified cause and divided patients into high and low groups in terms of climate predictors. Then we defined the cut-off values of parameters showing significant differences in the incidence of the acute symptom of chronic periodontal disease. The incidence of the acute symptom of chronic periodontal disease with unidentified cause was significantly different when the cases were classified according to the maximum hourly decrease in barometric pressure (1.5 and 1.9 hPa) (p = 0.04 and p = 0.03, respectively). This suggests that climate variables could be predictors of the acute symptom of chronic periodontal disease. Therefore, gaining a better understanding of these factors could help periodontal patients undergoing SPT prepare to avoid the acute symptom of chronic periodontal disease.

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  • Change in tongue pressure and the related factors after esophagectomy: a short-term, longitudinal study. Reviewed

    Aya Yokoi, Daisuke Ekuni, Reiko Yamanaka, Hironobu Hata, Yasuhiro Shirakawa, Manabu Morita

    Esophagus : official journal of the Japan Esophageal Society   16 ( 3 )   300 - 308   2019.7

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    BACKGROUND: Dysphagia is a prominent symptom after esophagectomy and may cause aspiration pneumonia. Swallowing evaluation after esophagectomy can predict and help control the incidence of postoperative pneumonia. The aim of this study was to clarify whether the change in tongue pressure was associated with any related factor and postoperative dysphagia/pneumonia in patients with esophageal cancer after esophagectomy. METHODS: Fifty-nine inpatients (41 males and 18 females; 33-77 years old) who underwent esophagectomy participated in this study. Measurement of tongue pressure and the repetitive saliva swallowing test (RSST) was performed before esophagectomy (baseline) and at 2 weeks postoperatively. The general data were collected from patients' medical records, including sex, age, type of cancer, cancer stage, location of cancer, operative approach, history of previous chemotherapy, surgical duration, amount of bleeding during surgery, incidences of postoperative complications, intubation period, period between surgery and initiation of oral alimentation, and intensive care unit (ICU) stay, blood chemical analysis, and lifestyle. RESULTS: Tongue pressure decreased significantly after esophagectomy (p = 0.011). The decrease of tongue pressure was significantly associated with length of ICU stay and preoperative tongue pressure on multiple regression analysis (p < 0.05). The decrease of tongue pressure in the RSST < 3 or postoperative pneumonia (+) group was significantly greater than in the RSST ≥ 3 (p = 0.003) or pneumonia (-) group (p = 0.021). CONCLUSIONS: The decrease in tongue pressure was significantly associated with the length of ICU stay, preoperative tongue pressure, and the incidence of dysphagia and pneumonia among inpatient after esophagectomy.

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  • Relationship between acetaldehyde concentration in mouth air and characteristics of microbiota of tongue dorsum in Japanese healthy adults: a cross-sectional study. Reviewed International journal

    Aya Yokoi, Daisuke Ekuni, Hironobu Hata, Mayu Yamane-Takeuchi, Takayuki Maruyama, Reiko Yamanaka, Manabu Morita

    Journal of applied oral science : revista FOB   27   e20180635   2019.6

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    OBJECTIVE: Acetaldehyde, associated with consumption of alcoholic beverages, is known to be a carcinogen and to be related to the tongue dorsum. The aim of this study was to investigate the relationship between acetaldehyde concentration in mouth air and bacterial characteristics on the tongue dorsum. METHODOLOGY: Thirty-nine healthy volunteers participated in the study. Acetaldehyde concentrations in mouth air were evaluated by a high-sensitivity semiconductor gas sensor. A 16S rRNA gene sequencing technique was used to compare microbiomes between two groups, focusing on the six samples with the highest acetaldehyde concentrations (HG) and the six samples with lowest acetaldehyde concentrations (LG). RESULTS: Acetaldehyde concentration increased in correlation with the increase in bacterial count (p=0.048). The number of species observed in the oral microbiome of the HG was higher than that in the oral microbiome of the LG (p=0.011). The relative abundances of Gemella sanguinis, Veillonella parvula and Neisseria flavescens in the oral microbiome of the HG were higher than those in the oral microbiome of the LG (p<0.05). CONCLUSION: Acetaldehyde concentration in mouth air was associated with bacterial count, diversity of microbiome, and relative abundance of G. sanguinis, V. parvula, and N. flavescens.

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  • (第2回)永久歯の抜歯原因調査 概要と主な結果

    川戸 貴行, 上條 英之, 恒石 美登里, 高野 直久, 森田 学, 公益財団法人8020推進財団永久歯の抜歯原因調査作業委員会

    歯界展望   133 ( 6 )   1045 - 1048   2019.6

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  • Serum microRNAs and chronic periodontitis: A case-control study. Reviewed International journal

    Toshiki Yoneda, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Kohei Fujimori, Yoshio Sugiura, Manabu Morita

    Archives of oral biology   101   57 - 63   2019.5

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    OBJECTIVE: An association is present between periodontitis and rates of expression of certain microRNAs (miRNAs) in periodontal tissue. However, the association between periodontitis and miRNA levels in human serum is unknown. We performed a case-control study in patients with chronic periodontitis to investigate serum miRNA levels. DESIGN: We enrolled 30 healthy patients without periodontitis and 30 patients with chronic periodontitis. Participants underwent clinical examination, case selection, and a blood draw from the antecubital vein. Serum miRNA profiles were compared in samples from participants with and without chronic periodontitis using microarray and real-time PCR. RESULTS: Microarray demonstrated seven miRNAs that were expressed <1/1.5 or >1.5 in the control group compared to the periodontitis group (p < 0.05). Real-time PCR showed that hsa-miR-664a-3p, hsa-miR-501-5p, and hsa-miR-21-3p were higher in the periodontitis group than the control group (p < 0.05). CONCLUSION: Hsa-miR-664a-3p, hsa-miR-501-5p, and hsa-miR-21-3p are candidate serum biomarkers for chronic periodontitis.

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  • 光線力学療法を併用した歯周治療によって歯周状態が改善した一症例

    江國 大輔, 中島 千穂, 森田 学

    口腔衛生学会雑誌   69 ( 2 )   98 - 105   2019.4

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    本症例(女性、ベースライン時77歳7ヵ月)は、以前から重度歯周炎のため、Supportive Periodontal Therapy(SPT)を受けており、歯周状態は安定していた。しかし、#34部の歯周状態が悪化し、違和感を訴えるようになった。また、#45部の破折による排膿が認められた。そこで、従来のスケーリング・ルートプレーニング(SRP)に光線力学療法(Fotosan)を組み合わせることにした。ベースライン時に違和感を訴えている#34の動揺度は0で、頬側近心の歯周ポケット深さ(Probing Pocket Depth、PPD)は5mmであった。プロービング時の出血(Bleeding on probing、BOP)および排膿はなかった。#45の破折部の動揺度は0、PPDは9mmで、BOPおよび排膿が認められた。SRPと光線力学療法を併用した結果、3ヵ月後には主訴である#34部の違和感は消失した。頬側近心のPPDは2mmに軽減し、その後2年3ヵ月まで歯周状態は安定していた。一方、#45部の破折部のPPDは深いままであったが、排膿は消失しその後2年3ヵ月まで同症状は認められなかった。以上のことから、SRPと光線力学療法の併用により歯周病が改善し、SPT期における長期的な歯周組織の安定のためには、光線力学療法の応用が重要であることが示唆された。(著者抄録)

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  • The level of salivary lactate dehydrogenase as an indicator of the association between gingivitis and related factors in Japanese university students. Reviewed

    Ayano Taniguchi-Tabata, Daisuke Ekuni, Tetsuji Azuma, Toshiki Yoneda, Mayu Yamane-Takeuchi, Kota Kataoka, Hirofumi Mizuno, Hisataka Miyai, Yoshiaki Iwasaki, Manabu Morita

    Journal of oral science   61 ( 1 )   133 - 139   2019.3

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    The aim of this study was to investigate the association between the presence of gingivitis estimated using the salivary level of lactate dehydrogenase (LD) and related factors in young Japanese adults. Data from 1,915 participants (21.4 ± 2.5 years) were analyzed. Unstimulated saliva was collected from each participant and the salivary LD level was evaluated using a commercially available test kit with an integer scale ranging from 1 to 10. Gingivitis was defined as the LD level of ≥8. The number of permanent teeth, the simplified oral hygiene index (OHI-S), the presence of partially erupted molars and body mass index were recorded. Additionally, participants answered a questionnaire. The percentage of male participants, the number of permanent teeth, the OHI-S and the presence of partially erupted molars were higher, whereas the proportion receiving dental check-ups was lower in the gingivitis group (n = 88, 4.6%) than in the healthy group. Logistic regression analysis showed that gingivitis was significantly associated with OHI-S (OR: 2.68, 95% CI: 1.94-3.69) and receiving dental checkups (OR: 0.31, 95% CI: 0.10-0.99). The present findings indicated that the OHI-S and receiving dental checkups were significantly associated with gingivitis, as assessed by the salivary LD level, in this cohort.

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  • Detection of Salivary miRNAs Reflecting Chronic Periodontitis: A Pilot Study. Reviewed International journal

    Kohei Fujimori, Toshiki Yoneda, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Hirofumi Mizuno, Yoshio Sugiura, Manabu Morita

    Molecules (Basel, Switzerland)   24 ( 6 )   2019.3

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    The purpose of this cross-sectional pilot study was to find salivary microRNAs (miRNAs) reflecting periodontal condition in chronic periodontitis. One hundred and twenty chronic periodontitis patients (mean age, 68.4 years) participated in the study, from whom unstimulated whole saliva was collected. A multiphase study was conducted to explore salivary miRNAs as biomarkers of periodontitis. At first, a polymerase chain reaction (PCR) array was performed to compare salivary miRNAs profiles in no and mild (no/mild) and severe periodontitis patients. Next, the relative expression of salivary miRNAs on individual samples was assessed by real-time reverse transcription-PCR. The numbers (%) of patients were 26 (21.6%, no/mild), 58 (48.3%, moderate) and 36 (30.0%, severe), respectively. Among 84 miRNAs, only the relative expression of hsa-miR-381-3p in the severe periodontitis group was significantly higher than that of the no/mild periodontitis group (p < 0.05). Among the 120 patients, there was also a significant correlation between the relative expression of hsa-miR-381-3p and the mean probing pocket depth (PPD) (r = 0.181, p < 0.05). Salivary hsa-miR-381-3p was correlated with periodontitis condition in chronic periodontitis patients.

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  • Awareness of Clenching and Underweight are Risk Factors for Onset of Crowding in Young Adults: A Prospective 3-Year Cohort Study. Reviewed International journal

    Naoki Toyama, Daisuke Ekuni, Ayano Taniguchi-Tabata, Kota Kataoka, Mayu Yamane-Takeuchi, Kohei Fujimori, Terumasa Kobayashi, Daiki Fukuhara, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

    International journal of environmental research and public health   16 ( 5 )   2019.2

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    Bruxism is a parafunctional activity that can seriously affect quality of life. Although bruxism induces many problems in the oral and maxillofacial area, whether it contributes to the onset of malocclusion remains unclear. The purpose of this prospective cohort study was to investigate the association between the onset of malocclusion and awareness of clenching during the daytime in young adults. Among 1,092 Okayama University students who underwent normal occlusion at baseline, we analysed 238 who had undergone a dental examination and had complete data after 3 years (2013⁻2016). We also performed subgroup analysis to focus on the association between awake bruxism and the onset of crowding (n = 216). Odds ratios (ORs) were calculated using multivariate logistic regression analyses. The incidences of malocclusion and crowding were 53.8% and 44.5%, respectively. In multivariate logistic regression, awareness of clenching was a risk factor for crowding (OR: 3.63; 95% confidence interval [CI]: 1.08⁻12.17). Moreover, underweight (body mass index < 18.5 kg/m²) was related to the onset of malocclusion (OR: 2.34; 95%CI: 1.11⁻4.92) and crowding (OR: 2.52, 95%CI: 1.25⁻5.76). These results suggest that awareness of clenching during the daytime and underweight are risk factors for the onset of crowding in young adults.

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  • Efficacy of Oral Care Provided by Interprofessional Collaboration for a Patient with Esophageal Cancer Associated with Post-polio Syndrome during Neoadjuvant Chemotherapy.

    Keiko Takahashi-Arimasa, Reiko Kohno-Yamanaka, Yoshihiko Soga, Rumi Miura, Manabu Morita

    Acta medica Okayama   73 ( 1 )   71 - 76   2019.2

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    Preoperative oral care is helpful to prevent postoperative complications in patients who are undergoing esophagectomy. Here, we report the case of an 81-year-old Japanese man with an upper limb disability caused by post-polio syndrome who was receiving neoadjuvant chemotherapy for esophageal cancer. He had poor oral health status and developed oral complications as a side effect of chemotherapy. He could not brush his teeth by himself. However, infection control by oral care provided by an interprofessional collaboration successfully improved his oral hygiene, and his follow-up involved no severe complications. Interprofessional collaboration is useful especially for patients with upper limb disability.

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  • Relationship Between Renal Dysfunction and Oral Mucositis in Patients Undergoing Concurrent Chemoradiotherapy for Pharyngeal Cancer: A Retrospective Cohort Study. Reviewed

    Mizuno H, Miyai H, Yokoi A, Kobayashi T, Inabu C, Maruyama T, Ekuni D, Mizukawa N, Kariya S, Nishizaki K, Kimata Y, Morita M

    In vivo (Athens, Greece)   33 ( 1 )   183 - 189   2019.1

  • 1歳6ヵ月児健康診査における萠出歯数の33年間の推移と萠出歯数に関連した因子の検討 Reviewed

    三好 健太郎, 高橋 大郎, 栗田 啓子, 本郷 博久, 竹原 順次, 中村 公也, 三宅 亮, 兼平 孝, 森田 学

    口腔衛生学会雑誌   69 ( 1 )   34 - 42   2019.1

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    本研究では、1歳6ヵ月児健康診査における乳歯の萠出歯数の33年間の推移と、萠出歯数に関する因子の解析を目的とした。対象は1980年から2012年まで北海道江別市の1歳6ヵ月児健康診査の受診児、27,454名である。歯科健康診査と身体計測結果に基づき、男女別に、年次ごとの1人平均萠出乳歯数、16歯以上保有者割合、癒合歯保有者割合を算出し、年次との関係を回帰直線で求めた。さらに、出生年、性別、出生順位、出生体重、1歳6ヵ月時の身長、同胸囲、癒合歯数、母親の年齢を説明変数、16歯以上萠出の有無を従属変数としてロジスティック回帰分析を行った。その結果、1)出生時体重、1歳6ヵ月時の体重、同身長、同胸囲、1人平均萠出乳歯数、および16歯以上保有者割合は、男女とも経年的に減少した。年次(x)から1人平均萠出歯数(y)を求める回帰直線の傾きは男児-0.0188、女児-0.0181であった。2)1人平均萠出歯数は男児が女児より多い傾向にあった。3)癒合歯保有者割合は年々増加する傾向にあり、男児のほうが女児よりもその傾向が強かった。4)16歯以上萠出については出生体重、1歳6ヵ月時身長、1歳6ヵ月時体重と有意な関連がみられた。以上から、ここ33年間では、乳歯の萠出が遅れる傾向にあり、乳歯の萠出には児の出生体重や1歳6ヵ月時身長、1歳6ヵ月時体重が関連している可能性が示唆された。(著者抄録)

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  • Characteristics of the Salivary Microbiota in Patients With Various Digestive Tract Cancers. Reviewed

    Kageyama S, Takeshita T, Takeuchi K, Asakawa M, Matsumi R, Furuta M, Shibata Y, Nagai K, Ikebe M, Morita M, Masuda M, Toh Y, Kiyohara Y, Ninomiya T, Yamashita Y

    Frontiers in microbiology   10   1780   2019

  • New oral hygiene care regimen reduces postoperative oral bacteria count and number of days with elevated fever in ICU patients with esophageal cancer. Reviewed

    Hirofumi Mizuno, Shinsuke Mizutani, Daisuke Ekuni, Ayano Tabata-Taniguchi, Takayuki Maruyama, Aya Yokoi, Chie Omori, Kazuyoshi Shimizu, Hiroshi Morimatsu, Yasuhiro Shirakawa, Manabu Morita

    Journal of oral science   60 ( 4 )   536 - 543   2018.12

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    Using a controlled pre/post study design, we investigated the effects of professional mechanical cleaning of the oral cavity with benzethonium chloride, interdental brushes, and hydrogen peroxide on the number of oral bacteria and postoperative complications among esophageal cancer patients in an intensive care unit. Before surgery, 44 patients with esophageal cancer were recruited at Okayama Hospital from January through August 2015. The control group (n = 23) received routine oral hygiene care in the intensive care unit. The intervention group (n = 21) received intensive interdental cleaning with benzethonium chloride solution and tongue cleaning with hydrogen peroxide. The number of oral bacteria on the tongue surface and plaque index were significantly lower in the intervention group than in the control group on postoperative days 1 and 2 (P < 0.05). Additionally, the number of days with elevated fever during a 1-week period was significantly lower in the intervention group than in the control group (P = 0.037). As compared with routine oral hygiene, a new oral hygiene regimen comprising benzethonium chloride, interdental brushes, and hydrogen peroxide significantly reduced the number of oral bacteria and days with elevated fever in patients with esophageal cancer.

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  • 歯周治療における禁煙支援の手順書 Reviewed

    稲垣 幸司, 内藤 徹, 石原 裕一, 金子 高士, 中山 洋平, 山本 龍生, 吉成 伸夫, 森田 学, 栗原 英見

    日本歯周病学会会誌   60 ( 4 )   201 - 219   2018.12

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    日本歯周病学会では2006年に定めた歯周病分類システムの中で、「喫煙は歯周病の最大の環境リスクファクターである」という認識に基づき、リスクファクターによる歯周炎の分類の1つとして喫煙関連歯周炎を提示した。喫煙が関連する歯周炎に対する歯周治療において、患者の喫煙状況の確認、喫煙者への喫煙の健康障害についての情報提供による禁煙支援は、歯周治療の反応や予後を良好に維持するため、重要である。本論文では、喫煙に関連する国情、喫煙者の動向、禁煙支援教育の現状、歯科における禁煙支援の効果に関するエビデンスおよび歯周治療における禁煙支援の手順を概説する。(著者抄録)

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  • Does Instruction of Oral Health Behavior for Workers Improve Work Performance?-Quasi-Randomized Trial. Reviewed International journal

    Naoki Toyama, Ayano Taniguchi-Tabata, Nanami Sawada, Yoshio Sugiura, Daiki Fukuhara, Yoko Uchida, Hisataka Miyai, Aya Yokoi, Shinsuke Mizutani, Daisuke Ekuni, Manabu Morita

    International journal of environmental research and public health   15 ( 12 )   2018.11

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    Oral disease can cause economic loss due to impaired work performance. Therefore, improvement of oral health status and prevention of oral disease is essential among workers. The purpose of this study was to investigate whether oral health-related behavioral modification intervention influences work performance or improves oral health behavior and oral health status among Japanese workers. We quasi-randomly separated participants into the intervention group or the control group at baseline. The intervention group received intensive oral health instruction at baseline and a self-assessment every three months. Both groups received oral examinations and answered the self-questionnaire at baseline and at one-year follow-up. At follow-up, the prevalence of subjects who use fluoride toothpastes and interdental brushes/dental floss were significantly higher in the intervention group than in the control group. Three variables (tooth brushing in workplace, using fluoride toothpaste, and experience of receiving tooth brushing instruction in a dental clinic) showed significant improvement only in the intervention group. On the other hand, work performance and oral status did not significantly change in either group. Our intensive oral health-related behavioral modification intervention improved oral health behavior, but neither work performance nor oral status, among Japanese workers.

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  • Impact of commensal flora on periodontal immune response to lipopolysaccharide. Reviewed International journal

    Daiki Fukuhara, Koichiro Irie, Yoko Uchida, Kota Kataoka, Kentaro Akiyama, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    Journal of periodontology   89 ( 10 )   1213 - 1220   2018.10

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    BACKGROUND: Commensal flora are involved in the appropriate development of the mature immune system. However, it is unclear how commensal flora contribute to immune responses against periodontal pathogens, including the response to lipopolysaccharide (LPS). The purpose of this study was to evaluate the expression of immune responses after topical application of LPS in germ-free (GF) and specific-pathogen-free (SPF) mice. METHODS: GF and SPF mice at 8 weeks of age were randomly divided into four groups each: a baseline group (n = 4/group) and three experimental groups (n = 6/group). Experimental groups received topical application of Porphyromonas gingivalis LPS (10 μg/μL) into the palatal gingival sulcus. Sampling was performed before LPS application (baseline) and at 3, 24, or 72 hours after LPS application. The numbers of neutrophils, CD4+ , and CD8+ T cells in periodontal tissue were evaluated by immunohistochemistry. Expression of genes encoding cytokines, chemokines, and a transcription factor was determined by real-time PCR. RESULTS: SPF mice, but not GF mice, showed an increased number of CD4+ T cells in the periodontal tissue at 3 hours after LPS application, compared with the number at baseline (p < 0.05). Gene expressions of tumor necrosis factor-α (Tnf-α) and forkhead box protein p3 (Foxp3) was also significantly higher in the SPF mice than in the GF mice at 3 hours after LPS application (p < 0.05). The number of neutrophils peaked at 24 hours in both GF and SPF mice. CONCLUSIONS: LPS-exposed SPF mice exhibited increases in the number of CD4+ T cells and in Tnf-α and Foxp3 gene expression in periodontal tissue compared with LPS-exposed GF mice.

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  • Unusual oral mucosal microbiota after hematopoietic cell transplantation with glycopeptide antibiotics: potential association with pathophysiology of oral mucositis Reviewed

    Misato Muro, Yoshihiko Soga, Tomoko Higuchi, Kota Kataoka, Daisuke Ekuni, Yoshinobu Maeda, Manabu Morita

    Folia Microbiologica   63 ( 5 )   587 - 597   2018.9

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    DOI: 10.1007/s12223-018-0596-1

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  • Commensal Microbiota Enhance Both Osteoclast and Osteoblast Activities. Reviewed International journal

    Yoko Uchida, Koichiro Irie, Daiki Fukuhara, Kota Kataoka, Takako Hattori, Mitsuaki Ono, Daisuke Ekuni, Satoshi Kubota, Manabu Morita

    Molecules (Basel, Switzerland)   23 ( 7 )   2018.6

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    Recent studies suggest that the commensal microbiota affects not only host energy metabolism and development of immunity but also bone remodeling by positive regulation of osteoclast activity. However, the mechanism of regulation of bone cells by the commensal microbiota has not been elucidated. In this study, 8-week-old specific pathogen-free (SPF) and germ-free (GF) mice were compared in terms of alveolar bones and primary osteoblasts isolated from calvarias. Micro-CT analysis showed that SPF mice had larger body size associated with lower bone mineral density and bone volume fraction in alveolar bones compared with GF mice. Greater numbers of osteoclasts in alveolar bone and higher serum levels of tartrate-resistant acid phosphatase 5b were observed in SPF mice. Tissue extracts from SPF alveolar bone showed higher levels of cathepsin K, indicating higher osteoclast activity. SPF alveolar extracts also showed elevated levels of γ-carboxylated glutamic acid⁻osteocalcin as a marker of mature osteoblasts compared with GF mice. Polymerase chain reaction (PCR) array analysis of RNA directly isolated from alveolar bone showed that in SPF mice, expression of mRNA of osteocalcin, which also acts as an inhibitor of bone mineralization, was strongly enhanced compared with GF mice. Cultured calvarial osteoblasts from SPF mice showed reduced mineralization but significantly enhanced expression of mRNAs of osteocalcin, alkaline phosphatase, insulin-like growth factor-I/II, and decreased ratio of osteoprotegerin/receptor activator of nuclear factor-kappa B ligand compared with GF mice. Furthermore, PCR array analyses of transcription factors in cultured calvarial osteoblasts showed strongly upregulated expression of Forkhead box g1. In contrast, Gata-binding protein 3 was strongly downregulated in SPF osteoblasts. These results suggest that the commensal microbiota prevents excessive mineralization possibly by stimulating osteocalcin expression in osteoblasts, and enhances both osteoblast and osteoclast activity by regulating specific transcription factors.

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  • Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium. Reviewed International journal

    Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Daiki Fukuhara, Yoko Uchida, Kota Kataoka, Shuichiro Kobayashi, Takeshi Kikuchi, Akio Mitani, Yoshihiro Shimazaki, Manabu Morita

    Archives of oral biology   87 ( 87 )   72 - 78   2018.3

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    OBJECTIVE: Increasing age is a potential risk factor for periodontal tissue breakdown, which may be affected by commensal flora. The aim of this study evaluated age-related changes in CD4+ T cells, C-C chemokine ligand 5 (CCL5), interleukin (IL)-17A, and receptor activator of nuclear factor-kappa B ligand (RANKL) expression using germ-free (GF) and conventionally reared (SPF) mice. DESIGN: GF and SPF mice at 8 (n = 6/group) and 22 weeks old (n = 6/group) were used. Immunohistochemical analyses were performed to determine the effects of aging on protein expression in periodontal tissues. Age-related changes in alveolar bone were quantified using micro-CT analysis. RESULTS: SPF mice, but not GF mice, showed an age-related increase in alveolar bone loss (P < 0.01). SPF mice at 22 weeks of age increased expression of CD4+ T cells, CCL5, IL-17A, and RANKL compared to those at 8 weeks of age in connective tissue and alveolar bone surface (P < 0.01). Furthermore, there was increased CD4+ T cells, which were co-expressed with IL-17A and RANKL in SPF mice at 22 weeks of age. On the other hand, the GF mice did not show any significant differences in CD4+ T cells, CCL5, IL-17A and RANKL expression between the two age groups. CONCLUSIONS: SPF mice induced an age-related increase in CD4+ T cells co- expressed with IL-17A and RANKL, with occurring alveolar bone loss. In contrast, GF mice did not show age-related changes in CD4+ T cell migration and cytokine expression.

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  • 職域における早食い、口腔内状態およびメタボリックシンドローム発症との関連 Reviewed

    横井 彩, 江國 大輔, 米田 俊樹, 森田 学

    口腔衛生学会雑誌   68 ( 1 )   9 - 14   2018.1

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    メタボリックシンドローム(Metabolic Syndrome:MetS)のリスク因子として早食いが挙げられる。また近年、MetsSは歯の喪失やう蝕など口腔内状態との関連も注目されている。しかし、MetS、早食いおよび口腔内状態を総合的に検討した報告は少ない。そこで、本縦断研究は、職域における早食いおよび口腔内状態とMetS発症との関連について検討することを目的とした。対象者は、某事業所における平成24・27年度定期健診をどちらも受診した従業員のうち、平成24年度MetSに該当しなかった男性114名(36〜63歳)を分析対象者とした。診査項目は、身長、体重、腹囲、血圧、空腹時血糖値、中性脂肪、HDLコレステロール値、口腔内状態、生活習慣(早食いの有無、食・運動・飲酒・喫煙習慣)とした。平成27年度健診結果から、MetS発症群と非発症群に分け、早食いの習慣および口腔内状態との関連を分析した。統計分析にはχ2検定、Fisherの直接法およびMann-Whitney U検定を用いた。3年後にMetSを発症した者(16名)は、全員早食いの自覚があった。また、MetS発症において、早食いと腹囲異常による相加効果がみられた。結論として、早食いで腹囲異常のある者はMetS発症のリスクが高いことがわかった。一方で、口腔内状態との直接的な関連はみられなかった。(著者抄録)

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  • Association between periodontitis and prognosis of pancreatobiliary tract cancer: A pilot study. Reviewed International journal

    Takayuki Maruyama, Takaaki Tomofuji, Tatsuya Machida, Hironari Kato, Koichiro Tsutsumi, Daisuke Uchida, Akinobu Takaki, Toshiki Yoneda, Hisataka Miyai, Hirofumi Mizuno, Daisuke Ekuni, Hiroyuki Okada, Manabu Morita

    Molecular and clinical oncology   6 ( 5 )   683 - 687   2017.5

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    Several studies have indicated that periodontitis is a risk factor for cancer. However, the association between periodontitis and the prognosis of pancreatobiliary tract cancer remains unclear. The aim of this pilot study was to investigate the association between periodontitis and prognosis of pancreatobiliary tract cancer. A total of 22 patients diagnosed with pancreatobiliary tract cancer were analyzed. Oral health status, including severity of periodontitis, general health status and biochemical serum markers were evaluated. The Kaplan-Meier method and Cox proportional hazards model were used to assess factors affecting the prognosis of pancreatobiliary tract cancer. The Kaplan-Meier analysis demonstrated that low body mass index, high concentration of serum C-reactive protein (CRP) and severe periodontitis were significant prognostic factors for survival rate. The Cox proportional hazards model revealed that serum carbohydrate antigen 19-9 concentration [hazard ratio (HR)=1.002; 95% confidence interval (CI): 1.000-1.004] and serum CRP concentration (HR=2.57; 95% CI: 1.15-5.74) were significantly associated with the prognosis of pancreatobiliary tract cancer. In addition, cancer patients with severe periodontitis had higher serum CRP concentrations compared with those without severe periodontitis. Therefore, severe periodontitis indirectly affected the prognosis of pancreatobiliary tract cancer through promoting systemic inflammation.

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  • The Serum Oxidative/Anti-oxidative Stress Balance Becomes Dysregulated in Patients with Non-alcoholic Steatohepatitis Associated with Hepatocellular Carcinoma. Reviewed

    Yasuyuki Shimomura, Akinobu Takaki, Nozomu Wada, Tetsuya Yasunaka, Fusao Ikeda, Takayuki Maruyama, Naofumi Tamaki, Daisuke Uchida, Hideki Onishi, Kenji Kuwaki, Shinichiro Nakamura, Kazuhiro Nouso, Yasuhiro Miyake, Kazuko Koike, Takaaki Tomofuji, Manabu Morita, Kazuhide Yamamoto, Hiroyuki Okada

    Internal medicine (Tokyo, Japan)   56 ( 3 )   243 - 251   2017.2

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    Objective Oxidative stress is associated with the progression of chronic liver disease. Non-alcoholic fatty liver disease (NAFLD) is also an oxidative stress-related disease. However, the oxidative/anti-oxidative balance has not been fully characterized in NAFLD. The objective of the present study was to investigate the balance between oxidative stress and the anti-oxidative activity in NAFLD, including non-alcoholic steatohepatitis (NASH)-related hepatocellular carcinoma (HCC). Patients We recruited 69 patients with histologically proven NAFLD without HCC (NAFLD; n=58), and with NASH-related HCC (NASH-HCC; n=11). The 58 NAFLD patients included patients with non-alcoholic fatty liver (NAFL; n=14) and NASH (n=44). Methods The serum levels of reactive oxygen metabolites (ROM) and anti-oxidative markers (OXY) were determined and then used to calculate the oxidative index. The correlations among such factors as ROM, OXY, oxidative index, and clinical characteristics were investigated. Results In NAFLD, ROM positively correlated with the body mass index (BMI), hemoglobin A1c (HbA1c), C-reactive protein (CRP), and the histological grade or inflammatory scores, while only high HbA1c and CRP levels were significant factors that correlated with a higher ROM according to a multivariate analysis. OXY positively correlated with the platelet counts, albumin, and creatinine levels, while negatively correlating with age. However, it improved after treatment intervention. The oxidative index positively correlated with BMI, CRP, and HbA1c. The NASH-HCC patients exhibited a lower OXY than the NASH patients, probably due to the effects of aging. Conclusion Oxidative stress correlated with the levels of NASH activity markers, while the anti-oxidative function was preserved in younger patients as well as in patients with a well-preserved liver function. The NASH-HCC patients tended to be older and exhibited a diminished anti-oxidative function.

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  • Associations among oral health-related quality of life, subjective symptoms, clinical status, and self-rated oral health in Japanese university students: a cross-sectional study. Reviewed International journal

    Mayu Yamane-Takeuchi, Daisuke Ekuni, Shinsuke Mizutani, Kota Kataoka, Ayano Taniguchi-Tabata, Tetsuji Azuma, Michiko Furuta, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

    BMC oral health   16 ( 1 )   127 - 127   2016.11

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    Background: The present study aimed to elucidate the associations among self-rated oral health, clinical oral health status, oral health behaviors, subjective oral symptoms, and oral health-related quality of life (OHRQoL) in a group of Japanese university students.
    Methods: Of 2051 participants, 2027 (98.83%) students received an optional oral examination and answered a questionnaire including items regarding age, sex, self-rated oral health, oral health behaviors, subjective oral symptoms, and OHRQoL [The Oral Health Impact Profile (OHIP)-14]. On oral examination, the decayed, missing, and filled teeth (DMFT) score, Community Periodontal Index (CPI), the percentage of teeth showing bleeding on probing (%BOP), and malocclusion were recorded. Structural equation modelling (SEM) analysis was used to test associations.
    Results: The mean score (+/- SD) of OHIP-14 was 1.92 +/- 5.47. In the SEM analysis, the final model showed that self-rated oral health, oral pain, malocclusion, and the DMFT score were directly associated with the OHRQoL, and subjective symptoms of temporomandibular disorders (TMD) and recurrent aphthous stomatitis were both directly and indirectly associated (p &lt; 0.05). CPI, %BOP, and oral health behaviors were excluded from the final model.
    Conclusions: OHRQoL was associated with self-related oral health, subjective symptoms of TMD, oral pain and stomatitis, DMFT, and malocclusion in this group of Japanese university students.

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  • Relationship Between Prehypertension/Hypertension and Periodontal Disease: A Prospective Cohort Study. Reviewed International journal

    Yuya Kawabata, Daisuke Ekuni, Hisataka Miyai, Kota Kataoka, Mayu Yamane, Shinsuke Mizutani, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

    American journal of hypertension   29 ( 3 )   388 - 96   2016.3

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    BACKGROUND
    Most cross-sectional studies have found a significant positive relationship between periodontal disease and prehypertension/hypertension. However, these studies had limitations and there are few prospective cohort studies in young adults. The purpose of this prospective cohort study was to investigate whether periodontal disease was related to prehypertension/hypertension in Japanese university students.
    METHODS
    Students (n = 2,588), who underwent health examinations before entering university and before graduation, were included in the analysis. The association between periodontal disease such as the percentage of bleeding on probing (BOP) and community periodontal index (CPI) scores, and change in blood pressure status was determined.
    RESULTS
    At the reexamination, the numbers of participants with prehypertension (systolic blood pressure 120-139 mm Hg or diastolic blood pressure 80-89 mm Hg) and hypertension (&gt;= 140/90 mm Hg) were 882 (34.1%) and 109 (4.2%), respectively. In a logistic regression model, the risk of hypertension was significantly associated with male (odds ratio (OR): 6.31; 95% confidence interval (CI): 2.63-15.13; P &lt; 0.001), no habitual physical activity at baseline (OR: 2.90; 95% CI: 1.56-5.38; P &lt; 0.01) and periodontal disease defined as the presence of both probing pocket depth (PPD) &gt;= 4 m and BOP &gt;= 30% at baseline (OR: 2.74; 95% CI: 1.19-6.29; P = 0.02) in participants with prehypertension at baseline. On the other hand, the risk of prehypertension was not associated with presence of periodontal disease (OR: 0.93; 95% CI: 0.51-1.70; P = 0.82).
    CONCLUSION
    In the short-term prospective cohort study, a significant association between presence of periodontal disease and hypertension was observed in Japanese university students.

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  • Association between Knowledge about Comprehensive Food Education and Increase in Dental Caries in Japanese University Students: A Prospective Cohort Study. Reviewed International journal

    Muneyoshi Kunitomo, Daisuke Ekuni, Shinsuke Mizutani, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Mayu Yamane, Kota Kataoka, Ayano Taniguchi-Tabata, Hirofumi Mizuno, Hisataka Miyai, Yoshiaki Iwasaki, Manabu Morita

    Nutrients   8 ( 3 )   114 - 114   2016.2

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    In Japan, comprehensive food education (shokuiku) programs are carried out with the aim of improving dietary practices and thereby reducing the incidence of lifestyle-related diseases, including dental caries. The purpose of this prospective cohort study was to investigate the association between knowledge about shokuiku and the increase in dental caries among Japanese university students who had attended a shokuiku program while in junior/senior high school. A total of 562 students volunteered to undergo oral examinations over a three-year follow-up period, during which the number of cases of dental caries were recorded. Additional information was collected using a questionnaire survey regarding knowledge about shokuiku, dietary habits, and oral health behaviors. In logistic regression analysis, males who lacked knowledge about shokuiku had significantly higher odds for dental caries than those who did not (odds ratio (OR), 2.00; 95% confidence interval (CI), 1.12-3.58; p = 0.019). On the other hand, among females, those who frequently consumed sugar-sweetened soft drinks had significantly higher odds for dental caries than those who did not (OR, 1.89; 95% CI, 1.05-3.42; p = 0.035). These results suggest that having no knowledge about shokuiku is associated with a risk of increase in dental caries in Japanese male university students.

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  • MicroRNAs in Salivary Exosome as Potential Biomarkers of Aging. Reviewed International journal

    Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Takayuki Maruyama, Toshiki Yoneda, Yuya Kawabata, Hirofumi Mizuno, Hisataka Miyai, Muneyoshi Kunitomo, Manabu Morita

    International journal of molecular sciences   16 ( 9 )   21294 - 309   2015.9

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    The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.

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  • Relationship between Acute Phase of Chronic Periodontitis and Meteorological Factors in the Maintenance Phase of Periodontal Treatment: A Pilot Study. Reviewed International journal

    Noriko Takeuchi, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    International journal of environmental research and public health   12 ( 8 )   9119 - 30   2015.8

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    The acute phase of chronic periodontitis may occur even in patients during supportive periodontal therapy. However, the details are not fully understood. Since the natural environment, including meteorology affects human health, we hypothesized that weather conditions may affect occurrence of acute phase of chronic periodontitis. The aim of this study was to investigate the relationship between weather conditions and acute phase of chronic periodontitis in patients under supportive periodontal therapy. Patients who were diagnosed with acute phase of chronic periodontitis under supportive periodontal therapy during 2011-2013 were selected for this study. We performed oral examinations and collected questionnaires and meteorological data. Of 369 patients who experienced acute phase of chronic periodontitis, 153 had acute phase of chronic periodontitis without direct-triggered episodes. When using the autoregressive integrated moving average model of time-series analysis, the independent covariant of maximum hourly range of barometric pressure, maximum hourly range of temperature, and maximum daily wind speed were significantly associated with occurrence of acute phase of chronic periodontitis (p &lt; 0.05), and 3.1% of the variations in these occurrence over the study period were explained by these factors. Meteorological variables may predict occurrence of acute phase of chronic periodontitis.

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  • Structural equation modeling to assess gender differences in the relationship between psychological symptoms and dental visits after dental check-ups for university students. Reviewed International journal

    Shinsuke Mizutani, Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

    Acta odontologica Scandinavica   73 ( 5 )   368 - 74   2015.7

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    Objective. Some studies have shown a relationship between psychological symptoms and oral health behaviors. However, it is unknown whether gender differences affect the relationship between psychological symptoms and oral health behaviors. In addition, gender differences in the relationship between dental anxiety and dental visits for treatment or regular check-up are unclear. The objective of the present study was to explain the relationships among gender differences, psychological symptoms, oral health behaviors, dental anxiety and 'expectation of dental visit', evaluated as 'dental visits when treatments are recommended' in university students. Materials and methods. A total of 607 students (311 males, 296 females) aged 18-38 years old were examined. The information was collected via questionnaire regarding gender, psychological symptoms and oral health behaviors. Psychological symptoms were assessed using the Hopkins Symptom Checklist. Structural equation modeling was used to test pathways from these factors to 'expectation of dental visit'. Multiple-group modeling was also conducted to test for gender differences. Results. Psychological symptoms were related to low expectation of dental visit in females, but there was no such relationship in males. Oral health behaviors were related to expectation of dental visit in both genders. Conclusions. Psychological symptoms were directly related to expectation of dental visit in females and oral health behaviors were related to expectation of dental visit in both genders. To promote dental visits after dental check-ups at school, it might be necessary to improve oral health behaviors in both genders and to evaluate psychological symptoms, especially in females.

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  • Association Between Self-Reported Bruxism and Malocclusion in University Students: A Cross-Sectional Study. Reviewed

    Kota Kataoka, Daisuke Ekuni, Shinsuke Mizutani, Takaaki Tomofuji, Tetsuji Azuma, Mayu Yamane, Yuya Kawabata, Yoshiaki Iwasaki, Manabu Morita

    Journal of epidemiology   25 ( 6 )   423 - 30   2015.6

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    Objectives: Bruxism can result in temporomandibular disorders, oral pain, and tooth wear. However, it is unclear whether bruxism affects malocclusion. The aim of this study was to examine the association between self-reported bruxism and malocclusion in university students.
    Methods: Students (n = 1503; 896 men and 607 women) aged 18 and 19 years were examined. Malocclusion was defined using a modified version of the Index of Orthodontic Treatment Need. The presence of buccal mucosa ridging, tooth wear, dental impression on the tongue, palatal/mandibular torus, and the number of teeth present were recorded, as well as body mass index (BMI). Additional information regarding gender, awareness of bruxism, orthodontic treatment, and oral habits was collected via questionnaire.
    Results: The proportion of students with malocclusion was 32% (n = 481). The awareness of clenching in males with malocclusion was significantly higher than in those with normal occlusion (chi square test, P &lt; 0.01). According to logistic regression analysis, the probability of malocclusion was significantly associated with awareness of clenching (odds ratio [OR] 2.19; 95% confidence interval [CI], 1.22-3.93) and underweight (BMI &lt; 18.5 kg/m(2)) (OR 1.89; 95% CI, 1.31-2.71) in males but not in females. In subgroup analyses, the probability of crowding was also significantly associated with awareness of clenching and underweight (P &lt; 0.01) in males.
    Conclusions: Awareness of clenching and underweight were related to malocclusion (crowding) in university male students.

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  • Detection of Identical Isolates of Enterococcus faecalis from the Blood and Oral Mucosa in a Patient with Infective Endocarditis. Reviewed

    Akemi Okui, Yoshihiko Soga, Susumu Kokeguchi, Motoko Nose, Reiko Yamanaka, Nobuchika Kusano, Manabu Morita

    Internal medicine (Tokyo, Japan)   54 ( 14 )   1809 - 14   2015

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    The detection of infective endocarditis (IE) of oral origin has been previously discussed. However, there are few reports confirming this infection using molecular biological techniques. We herein describe the case of a 67-year-old man who developed IE. Blood culture samples and strains obtained from the gingival and buccal mucosa showed 100% identity to Enterococcus faecalis JCM 5803 on sequencing of 16S rRNA gene fragments. A random amplification of polymorphic DNA (RAPD) analysis showed the same pattern for these samples, thus confirming the identity of E. faecalis isolates in the blood and oral mucosa. Our observations provide novel information regarding the level of identity between IE pathogens and oral bacteria.

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  • Prevalence of tobacco smoking among members of the Japanese Society of Periodontology Reviewed

    稲垣幸司, 稲垣幸司, 王宝禮, 王宝禮, 埴岡隆, 埴岡隆, 藤井健男, 藤井健男, 両角俊哉, 両角俊哉, 伊藤弘, 伊藤弘, 山本龍生, 山本龍生, 森田学, 森田学

    日本歯周病学会会誌(Web)   57 ( 2 )   100‐106 (J‐STAGE)   2015

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  • Longitudinal relationship between plasma reactive oxygen metabolites and periodontal condition in the maintenance phase of periodontal treatment. Reviewed International journal

    Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Mayu Yamane, Toshiki Yoneda, Yuya Kawabata, Kota Kataoka, Naofumi Tamaki, Manabu Morita

    Disease markers   2014   489292 - 489292   2014.4

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    When plasma ROM level in periodontitis patients was low, increases in plasma ROM level were associated with those in CAL during the maintenance phase of treatment.

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  • Factors related to the formation of buccal mucosa ridging in university students. Reviewed International journal

    Shinsuke Mizutani, Daisuke Ekuni, Takaaki Tomofuji, Tetsuji Azuma, Koichiro Irie, Tatsuya Machida, Toshiki Yoneda, Yoshiaki Iwasaki, Manabu Morita

    Acta odontologica Scandinavica   72 ( 1 )   58 - 63   2014.1

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    Objective. Buccal mucosa ridging (BMR) is known as a clinical sign of clenching, which is one of the major manifestations of bruxism. However, there are few reports about the formation of BMR and no data regarding the association between BMR and factors such as malocclusion. The purpose of the current study was to investigate the relationship between BMR and factors such as the number of teeth present, gender, body mass index (BMI), occlusion and clenching habit in university students. Materials and methods. A total of 2101 students (1164 males, 937 females), aged 18-29 years old, were included in the study. BMR and the number of teeth present were recorded and malocclusion was defined using a modified version of the Index of Orthodontic Treatment Need. Additional information regarding gender, clenching habit and BMI was collected via a questionnaire. Results. Forty-six per cent of the subjects had BMR and the prevalence of BMR in females was significantly higher than that of males (chi square test, p &lt; 0.001). According to logistic regression analysis, the probability of BMR was significantly associated with female gender (OR = 1.501, 95% CI = 1.259-1.790, p &lt; 0.001), crowding (OR = 2.102, 95% CI = 1.706-2.590, p &lt; 0.001) and overjet (OR = 0.585, 95% CI = 0.418-0.818, p = 0.002). On the other hand, BMR was not associated with awareness of clenching habit and BMI. Conclusions. Gender, crowding and overjet were related to the formation of BMR in university students. When evaluating BMR as a clinical sign of clenching, one might have to take factors such as gender and crowding into consideration.

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  • Anti-ageing effects of dentifrices containing anti-oxidative, anti-inflammatory, and anti-bacterial agents (Tomarina®) on gingival collagen degradation in rats. Reviewed International journal

    Irie Koichiro, Takaaki Tomofuji, Daisuke Ekuni, Yasumasa Endo, Kenta Kasuyama, Tetsuji Azuma, Naofumi Tamaki, Toshiki Yoneda, Manabu Morita

    Archives of oral biology   59 ( 1 )   60 - 65   2014.1

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    Previous studies have demonstrated the relationship between ageing and oxidative stress. In this study, we examined the effects of topical application of a dentifrice containing anti-oxidative, anti-inflammatory, and anti-bacterial agents (Tomarina?) to the gingival surface on gingival collagen degradation in rats. Fischer 344 male rats (4 or 8 months old) were divided into two groups: experimental group and control group. Tomarina? (the experimental group) or control dentifrice (the control group) was applied 5 days per week for 2 months. In the control group, gingival collagen density decreased with ageing. In the experimental group, the collagen density did not change with ageing, and was greater than that in the control group at 10 months of age (p &lt; 0.0083). In addition, the control group showed an increase in serum oxidative stress with ageing. The experimental group also showed increased serum oxidative stress, but the value was lower than the control group at 10 months of age (p &lt; 0.0083). Furthermore, low expressions of protein oxidative damage in the periodontal tissue were observed in the experimental group, compared to the control group at 6 months and 10 months. These findings indicate that Tomarina? might suppress the effects of ageing on gingival collagen degradation, by decreasing oxidative stress in the rat model.

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  • 食道癌患者のより良い周術期医療のために歯科はどのような貢献ができるのか? 周術期管理センター(PERIO) Reviewed

    山中 玲子, 曽我 賢彦, 前田 直見, 田辺 俊介, 大原 利章, 野間 和広, 白川 靖博, 森田 学, 佐藤 健治, 森松 博史, 藤原 俊義

    日本臨床外科学会雑誌   74 ( 増刊 )   478 - 478   2013.10

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  • Serum oxidative-anti-oxidative stress balance is dysregulated in patients with hepatitis C virus-related hepatocellular carcinoma. Reviewed International journal

    Mamoru Nishimura, Akinobu Takaki, Naofumi Tamaki, Takayuki Maruyama, Hideki Onishi, Sayo Kobayashi, Kazuhiro Nouso, Tetsuya Yasunaka, Kazuko Koike, Hiroaki Hagihara, Kenji Kuwaki, Shinichiro Nakamura, Fusao Ikeda, Yoshiaki Iwasaki, Takaaki Tomofuji, Manabu Morita, Kazuhide Yamamoto

    Hepatology research : the official journal of the Japan Society of Hepatology   43 ( 10 )   1078 - 92   2013.10

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    AIM: Oxidative stress is associated with progression of chronic liver disease (CLD). This association is best established in chronic hepatitis C. However, the anti-oxidative state is not well characterized. The objective of the present study was to investigate the balance of oxidative and anti-oxidative stress in CLD patients. METHODS: We recruited a study population of 208 patients, including healthy volunteers (HV; n=15), patients with hepatitis B virus (HBV)-related CLD without or with hepatocellular carcinoma (HBV-non-HCC, n=25, and HBV-HCC, n=50, respectively), and patients with hepatitis C virus (HCV)-related CLD without or with HCC (HCV-non-HCC, n=49, and HCV-HCC, n=69, respectively). Serum levels of reactive oxygen metabolites (ROM) and anti-oxidative markers (OXY-adsorbent test; OXY) were determined, and the balance of these values was used as the oxidative index. Correlations among ROM, OXY, oxidative index and clinical characteristics were investigated. RESULTS: Patients with CLD exhibited elevated ROM and oxidative index compared to HV. Among patients with CLD, HCV positive status correlated with increased ROM. In CLD, HCV-HCC patients exhibited the highest ROM levels. Among HCV-related CLD patients, lower OXY correlated with HCC positive status, but was recovered by eradication of HCC. In HCV-HCC, lower OXY correlated with high PT-INR. CONCLUSION: HCV positive CLD patients displayed higher oxidative stress and HCV-HCC patients displayed lower anti-oxidative state. Anti-oxidative state depression was associated with liver reservoir-related data in HCV-HCC and could be reversed with HCC eradication.

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  • Involvement of toll-like receptor 2 and 4 in association between dyslipidemia and osteoclast differentiation in apolipoprotein E deficient rat periodontium. Reviewed International journal

    Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Kenta Kasuyama, Toshiki Yoneda, Manabu Morita

    Lipids in health and disease   12   1 - 1   2013.1

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    Background: Dyslipidemia increases circulating levels of oxidized low-density lipoprotein (OxLDL) and this may induce alveolar bone loss through toll-like receptor (TLR) 2 and 4. The purpose of this study was to investigate the effects of dyslipidemia on osteoclast differentiation associated with TLR2 and TLR4 in periodontal tissues using a rat dyslipidemia (apolipoprotein E deficient) model.
    Methods: Levels of plasma OxLDL, and the cholesterol and phospholipid profiles in plasma lipoproteins were compared between apolipoprotein E-deficient rats (16-week-old males) and wild-type (control) rats. In the periodontal tissue, we evaluated the changes in TLR2, TLR4, receptor activator of nuclear factor kappa B ligand (RANKL) and tartrate resistant acid phosphatase (TRAP) expression.
    Results: Apolipoprotein E-deficient rats showed higher plasma levels of OxLDL than control rats (p&lt;0.05), with higher plasma levels of total cholesterol (p&lt;0.05) and LDL-cholesterol (p&lt;0.05) and lower plasma levels of high-density lipoprotein cholesterol (p&lt;0.05). Their periodontal tissue also exhibited a higher ratio of RANKL-positive cells and a higher number of TRAP-positive osteoclasts than control rats (p&lt;0.05). Furthermore, periodontal gene expression of TLR2, TLR4 and RANKL was higher in apolipoprotein E-deficient rats than in control rats (p&lt;0.05).
    Conclusion: These findings underscore the important role for TLR2 and TLR4 in mediating the osteoclast differentiation on alveolar bone response to dyslipidemia.

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  • Lipopolysaccharide induces a stromal-epithelial signalling axis in a rat model of chronic periodontitis. Reviewed International journal

    James D Firth, Daisuke Ekuni, Koichiro Irie, Takaaki Tomofuji, Manabu Morita, Edward E Putnins

    Journal of clinical periodontology   40 ( 1 )   8 - 17   2013.1

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    Aim Lipopolysaccharide is a bacterial virulence factor implicated in chronic periodontitis, which may penetrate the junctional epithelial barrier and basement membrane to insult underlying stroma. We sought to identify lipopolysaccharide-induced global gene expression changes responsible for signalling between stroma and epithelium during disease onset.
    Materials and Methods Using a rat lipopolysaccharide periodontitis model, junctional epithelium and underlying stromal tissue were separately collected from healthy and diseased animals by laser-capture microdissection and subject to gene expression microarray analysis. Key gene products identified were validated in gingival epithelial and fibroblast cell cultures.
    Results Global gene expression patterns distinguishing health versus disease were found in and between both tissue types. In stroma, the most significantly altered gene ontology function group (Z &gt;= 4.00) was cytokines, containing most significantly (+/- 2-fold; p &lt; 0.05) upregulated genes amphiregulin, IL1-beta and Fas ligand, all positive, diffusible modulators of the epithelial growth factor receptor pathway. In epithelium, the most significant changes were in downregulated FOS-related antigen-1 gene, somatostatin receptor-2 gene and mucin-4 gene, all negative modulators of the epithelial growth factor receptor pathway.
    Conclusion These results establish a periodontitis model for studying gene product interactions and suggests that the onset of junctional epithelial disease hyperproliferation involves a concerted stromalepithelial signalling axis.

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  • Relationships between eating habits and periodontal condition in university students. Reviewed International journal

    Takaaki Tomofuji, Michiko Furuta, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

    Journal of periodontology   82 ( 12 )   1642 - 9   2011.12

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    Background: Being overweight is a risk factor for peri odontitis. Unhealthy eating habits, which can induce overweight, may be involved in the development of periodontitis in young people. The present study aims to examine the relationships among overweight, eating habits, and the periodontal condition in Japanese university students.
    Methods: We conducted a cross-sectional study of 801 university students (413 males and 388 females; age range: 18 to 25 years). Patients were classified as underweight (body mass index [BMI] &lt; 18.5 kg/m(2)), normal weight (BMI of 18.5 to 22.9 kg/m(2)), and overweight (BMI &gt;= 23 kg/m(2)). Students completed a questionnaire including items related to eating habits and underwent oral health examinations. Patients with a community periodontal index (CPI) of 0 to 2 were considered controls, and patients with a CPI &gt; 2 were considered to have periodontitis.
    Results: The prevalence of underweight, normal weight, and overweight patients was 21%, 62%, and 17%, respectively. In overweight patients, the periodontitis risk was increased by the frequent consumption of fatty foods (adjusted odds ratio: 2.3; 95% confidence interval: 1.1 to 5.2; P &lt; 0.05) and reduced by the frequent consumption of vegetables (adjusted odds ratio: 0.2; 95% confidence interval: 0.1 to 0.7; P &lt; 0.01). In underweight and normal-weight groups, eating habits did not differ significantly according to the presence of periodontitis.
    Conclusions: In overweight students, the frequent consumption of fatty foods and infrequent consumption of vegetables were associated with an increased risk of periodontitis. In underweight and normal-weight students, eating habits had little effect on the periodontal condition. J Periodontol 2071; 82:1642-1649.

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  • Hydrogen-rich water attenuates experimental periodontitis in a rat model Reviewed

    Kenta Kasuyama, Takaaki Tomofuji, Daisuke Ekuni, Naofumi Tamaki, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Manabu Morita

    JOURNAL OF CLINICAL PERIODONTOLOGY   38 ( 12 )   1085 - 1090   2011.12

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    Aim: Reactive oxygen species (ROS) contribute to the development of periodontitis. As molecular hydrogen can act as a scavenger of ROS, we examined the effects of treatment with hydrogen-rich water on a rat model of periodontitis.
    Material & Methods: A ligature was placed around the maxillary molars for 4 weeks to induce periodontitis, and the animals were given drinking water with or without hydrogen-rich water.
    Results: The rats with periodontitis which were treated with pure water showed a time-dependent increase in serum ROS level. Compared with the rats without periodontitis, the periodontitis-induced rats which were given pure water also showed polymorphonuclear leucocyte infiltration and alveolar bone loss at 4 weeks. Hydrogen-rich water intake inhibited an increase in serum ROS level and lowered expression of 8-hydroxydeoxyguanosine and nitrotyrosine in the periodontal tissue at 4 weeks. Such conditions prevented polymorphonuclear leucocyte infiltration and osteoclast differentiation following periodontitis progression. Furthermore, inflammatory signalling pathways, such as mitogen-activated protein kinases, were less activated in periodontal lesions from hydrogen-rich water-treated rats as compared with pure water-treated rats.
    Conclusion: Consuming hydrogen-rich water might be beneficial in suppressing periodontitis progression by decreasing gingival oxidative stress.

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  • Hydrogen-rich water attenuates experimental periodontitis in a rat model. Reviewed International journal

    Kenta Kasuyama, Takaaki Tomofuji, Daisuke Ekuni, Naofumi Tamaki, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Manabu Morita

    Journal of clinical periodontology   38 ( 12 )   1085 - 90   2011.12

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    Consuming hydrogen-rich water might be beneficial in suppressing periodontitis progression by decreasing gingival oxidative stress.

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  • Periodontal treatment decreases plasma oxidized LDL level and oxidative stress Reviewed

    Naofumi Tamaki, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Manabu Morita

    CLINICAL ORAL INVESTIGATIONS   15 ( 6 )   953 - 958   2011.12

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    Periodontitis induces excessive production of reactive oxygen species in periodontal lesions. This may impair circulating pro-oxidant/anti-oxidant balance and induce the oxidation of low-density lipoprotein (LDL) in blood. The purpose of this study was to monitor circulating oxidized LDL and oxidative stress in subjects with chronic periodontitis following non-surgical periodontal treatment. Plasma levels of oxidized LDL and oxidative stress in 22 otherwise healthy non-smokers with chronic periodontitis (mean age 44.0 years) were measured at baseline and at 1 and 2 months after non-surgical periodontal treatment. At baseline, chronic periodontitis patients had higher plasma levels of oxidized LDL and oxidative stress than healthy subjects (p &lt; 0.001). Periodontal treatment was associated with a significant reduction in plasma levels of oxidized LDL (oxLDL)(p &lt; 0.001) and oxidative stress (p &lt; 0.001). At 2 months after periodontal treatment, the degree of change in the oxLDL was positively correlated with that in the oxidative stress (r = 0.593, p = 0.004). These observations indicate that periodontitis patients showed higher levels of circulating oxLDL and oxidative stress than healthy subjects. In addition, improved oral hygiene and non-surgical periodontal treatment were effective in decreasing oxLDL, which was positively associated with a reduction in circulating oxidative stress.

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  • Periodontal treatment decreases plasma oxidized LDL level and oxidative stress. Reviewed International journal

    Naofumi Tamaki, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Manabu Morita

    Clinical oral investigations   15 ( 6 )   953 - 8   2011.12

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    Periodontitis induces excessive production of reactive oxygen species in periodontal lesions. This may impair circulating pro-oxidant/anti-oxidant balance and induce the oxidation of low-density lipoprotein (LDL) in blood. The purpose of this study was to monitor circulating oxidized LDL and oxidative stress in subjects with chronic periodontitis following non-surgical periodontal treatment. Plasma levels of oxidized LDL and oxidative stress in 22 otherwise healthy non-smokers with chronic periodontitis (mean age 44.0 years) were measured at baseline and at 1 and 2 months after non-surgical periodontal treatment. At baseline, chronic periodontitis patients had higher plasma levels of oxidized LDL and oxidative stress than healthy subjects (p &lt; 0.001). Periodontal treatment was associated with a significant reduction in plasma levels of oxidized LDL (oxLDL)(p &lt; 0.001) and oxidative stress (p &lt; 0.001). At 2 months after periodontal treatment, the degree of change in the oxLDL was positively correlated with that in the oxidative stress (r = 0.593, p = 0.004). These observations indicate that periodontitis patients showed higher levels of circulating oxLDL and oxidative stress than healthy subjects. In addition, improved oral hygiene and non-surgical periodontal treatment were effective in decreasing oxLDL, which was positively associated with a reduction in circulating oxidative stress.

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  • Stage of hepatocellular carcinoma is associated with periodontitis. Reviewed International journal

    Naofumi Tamaki, Akinobu Takaki, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Daisuke Ekuni, Tetsuya Yasunaka, Kazuhide Yamamoto, Manabu Morita

    Journal of clinical periodontology   38 ( 11 )   1015 - 20   2011.11

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    HCC patients with periodontitis had higher JIS score and circulating ROS level than HCC patients without periodontitis.

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  • Stage of hepatocellular carcinoma is associated with periodontitis Reviewed

    Naofumi Tamaki, Akinobu Takaki, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Daisuke Ekuni, Tetsuya Yasunaka, Kazuhide Yamamoto, Manabu Morita

    JOURNAL OF CLINICAL PERIODONTOLOGY   38 ( 11 )   1015 - 1020   2011.11

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    Aim: Periodontitis induces overproduction of reactive oxygen species (ROS). This state increases circulating ROS levels and may affect hepatocellular carcinoma (HCC). The Japan Integrated Stage (JIS) score is a novel staging system for HCC. The objective of the present study was to compare JIS scores in HCC patients with and without periodontitis.
    Material and Methods: We recruited 64 HCC patients comprising 31 chronic periodontitis subjects (HCC + P) and 33 periodontally healthy controls (HCC + H). Their JIS scores were recorded. Serum levels of reactive oxygen metabolites (ROM) from HCC + P, HCC + H and healthy age-and gender-matched subjects with healthy gingiva (control, n = 15) were also assessed for circulating ROS levels.
    Results: The HCC + P and HCC + H groups had similar body mass index, habitual drinking and tobacco exposure data. The HCC + P group showed higher JIS scores than the HCC + H group (p = 0.027). Both the HCC + P and HCC + H groups had higher serum levels of ROM than controls (p &lt; 0.001), while serum levels of ROM in the HCC + P group were a further 25.8% higher than those in the HCC + H group (p &lt; 0.001).
    Conclusion: HCC patients with periodontitis had higher JIS score and circulating ROS level than HCC patients without periodontitis.

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  • Relationships between periodontal inflammation, lipid peroxide and oxidative damage of multiple organs in rats. Reviewed

    Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takayuki Maruyama, Tatsuo Yamamoto, Tatsuo Watanabe, Manabu Morita

    Biomedical research (Tokyo, Japan)   32 ( 5 )   343 - 9   2011.10

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    Gingival response to periodontal inflammation generates excessive lipid peroxide and such a condition may augment systemic health through increased circulating lipid peroxide. The purpose of the present study was to investigate whether the generation of lipid peroxide in periodontal inflammation could induce tissue injury in the liver, heart, kidney and brain using a rat model. Twelve Wistar rats (8 week-old male) were divided into 2 groups: the periodontal inflammation group, receiving topical application of lipopolysaccharide and proteases to the gingival sulcus for 4 weeks, and the control group using instead pyrogen-free water. After blood samples were collected, specimens from the brain, heart, liver and kidney were resected to determine the concentration of 8-hydroxydeoxyguanosine (an indicator of oxidative DNA damage). Gingival and serum levels for hexanoyl-lysine were measured to evaluate lipid peroxide. Administration of lipopolysaccharide and proteases induced periodontal inflammation, with increasing gingival and serum levels of hexanoyl- lysine. The level of 8-hydroxydeoxyguanosine increased 2.27, 2.01, 1.49 and 1.40 times in mitochondrial DNA from the liver, heart, kidney and brain of rats with periodontal inflammation, respectively. The results reveal that excessive production of lipid peroxide following periodontal inflammation is involved in oxidative DNA damage of the brain, heart, liver and kidney.

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  • 北海道における高齢者の歯の状況と全身の健康に関する研究 医科診療費からの分析

    葭内 朗裕, 兼平 孝, 栗田 啓子, 竹原 順次, 高橋 大郎, 本多 丘人, 秋野 憲一, 相田 潤, 森田 学

    北海道歯学雑誌   32 ( 1 )   12 - 24   2011.9

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    本研究は、北海道国民健康保険団体連合会(以下、&quot;北海道国保連合会&quot;と略)から提供を受けた平成19年5月のレセプトデータに基づき、北海道内の国民健康保険被保険者のうち、満70歳以上で歯科医療機関を含む医療機関を受診した者を対象に、現在歯数、欠損補綴状況、歯周病罹患状況と被保険者1人あたりの医科診療費との関連およびレセプト1件あたりの医科診療費から現在歯数と生活習慣病の罹患状況との関連について調べたものである。その結果、平均医科診療費は、1)現在歯が20歯未満の高齢者は、20歯以上の高齢者に比べ、1.2〜1.3倍、中でも現在歯数が0〜4歯の高齢者は、20歯以上の高齢者に比べ、1.4〜1.6倍と有意に高いこと、2)歯の欠損部の補綴処置を受けていない高齢者は、受けている高齢者に比べ、1.1倍程度と統計学的に有意ではないがやや高い傾向にあること、3)重度の歯周病を有する高齢者は、歯周病がない、あるいは軽度の高齢者に比べ、統計学的に有意ではないが1.1〜1.3倍程度とやや高い傾向にあること、などが明らかとなった。また、生活習慣病による平均医科診療費(レセプト1件あたり)は、1)現在歯が20歯未満の高齢者は、20歯以上の高齢者に比べて1.1〜1.3倍、中でも現在歯数が0〜4歯の高齢者は、20歯以上の高齢者に比べて1.2〜1.7倍と有意に高かった、2)悪性腫瘍、糖尿病、高血圧性疾患、虚血性心疾患、脳血管障害では、現在歯が20歯未満の高齢者は、20歯以上の高齢者に比べるとそれぞれ1.1〜1.4倍、中でも現在歯数が0〜4歯の高齢者は、20歯以上の高齢者に比べ、1.4〜1.7倍といずれも有意に高かった。(著者抄録)

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  • Effects of exercise training on gingival oxidative stress in obese rats Reviewed

    Tetsuji Azuma, Takaaki Tomofuji, Yasumasa Endo, Naofumi Tamaki, Daisuke Ekuni, Koichiro Irie, Kenta Kasuyama, Tomo Kato, Manabu Morita

    ARCHIVES OF ORAL BIOLOGY   56 ( 8 )   768 - 774   2011.8

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    Objective: The purpose of the present study was to investigate the effects of exercise training on serum reactive oxygen species (ROS) level and gingival oxidative stress in obese rats fed a high-fat diet.
    Design: Rats were divided into three groups (n = 14/group): one control group (fed a regular diet) and two experimental groups (fed a high-fat diet with and without exercise training [treadmill: 5 days/week]). The rats were sacrificed at 4 or 8 weeks. The level of serum reactive oxidative metabolites (ROM) was measured as an indicator of circulating ROS. The level of 8-hydroxydeoxyguanosine (8-OHdG) and reduced-form glutathione (GSH)/oxidised-form glutathione (GSSG) ratio were determined to evaluate gingival oxidative stress.
    Results: The obese rats fed a high-fat diet without exercise training showed higher serum ROM levels [Carratelli Units (CARR U)] (mean +/- SD; 413 +/- 64) than the control (333 +/- 12) at 4 weeks (p = 0.023). Such a condition resulted in higher 8-OHdG levels (ng/mg mtDNA) (0.97 +/- 0.18) (p &lt; 0.05) and a lower GSH/GSSG ratio (17.0 +/- 3.1) (p &lt; 0.05) in gingival tissues, compared to the control (0.55 +/- 0.13 for 8-OHdG and 23.6 +/- 5.8 for GSH/GSSG ratio) at 8 weeks. In addition, the obese rats fed a high-fat diet with exercise training showed lower serum ROM (623 +/- 103) (p&lt;0.001) and gingival 8-OHdG levels (0.69 +/- 0.17) (p = 0.012) than those without exercise training (1105 95 for ROM and 0.55 +/- 0.13 for 8-OHdG) at 8 weeks.
    Conclusions: Obesity prevention by exercise training may effectively suppress gingival oxidative stress by decreasing serum ROS in rats. (C) 2011 Elsevier Ltd. All rights reserved.

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  • Effects of exercise training on gingival oxidative stress in obese rats. Reviewed International journal

    Tetsuji Azuma, Takaaki Tomofuji, Yasumasa Endo, Naofumi Tamaki, Daisuke Ekuni, Koichiro Irie, Kenta Kasuyama, Tomo Kato, Manabu Morita

    Archives of oral biology   56 ( 8 )   768 - 74   2011.8

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    Obesity prevention by exercise training may effectively suppress gingival oxidative stress by decreasing serum ROS in rats.

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  • A screening test for capsaicin-stimulated salivary flow using filter paper: a study for diagnosis of hyposalivation with a complaint of dry mouth. International journal

    Takashi Kanehira, Tomotaka Yamaguchi, Kozo Asano, Manabu Morita, Etsuko Maeshima, Akemi Matsuda, Yoshihiro Fujii, Wataru Sakamoto

    Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics   112 ( 1 )   73 - 80   2011.7

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    OBJECTIVE: The purpose of this study was to develop a simple screening technique for diagnosis of hyposalivation with dry mouth by estimation of capsaicin-stimulated salivary flow using filter paper. STUDY DESIGN: An assay system comprising 5 spots containing starch and potassium iodide on filter paper incorporating or without capsaicin and a coloring reagent was designed. We investigated whether the number of colored spots using the filter paper incorporating capsaicin could distinguish between healthy subjects and subjects with hyposalivation and dry mouth. RESULTS: In the healthy group (>200 μL/min; n = 33), the capsaicin-stimulated salivary flow significantly increased as compared with the resting salivary flow, from 1.2 ± 1.4 to 2.9 ± 1.3 colored spots (P < .05). In contrast, the hyposalivation group with dry mouth (<100 μL/min; n = 32) hardly changed (4.4 ± 1.0 vs 4.9 ± 0.2), except for 3 subjects who had considerable elevated secretion on capsaicin stimulation. CONCLUSION: By measuring resting and stimulated salivary flows, this method should be useful for evaluating retained functional ability of salivary glands and screening of hyposalivation with dry mouth.

    DOI: 10.1016/j.tripleo.2011.02.036

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  • A new portable monitor for measuring odorous compounds in oral, exhaled and nasal air. Reviewed International journal

    Naofumi Tamaki, Kenta Kasuyama, Mitsue Esaki, Takara Toshikawa, Shun-Ichi Honda, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    BMC oral health   11   15 - 15   2011.4

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    ClinicalTrials.gov: NCT01139073.

    DOI: 10.1186/1472-6831-11-15

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  • Sex differences in gingivitis relate to interaction of oral health behaviors in young people. Reviewed International journal

    Michiko Furuta, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Toshio Ogura, Manabu Morita

    Journal of periodontology   82 ( 4 )   558 - 65   2011.4

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    Background: Although many epidemiologic surveys have shown that gingivitis is more prevalent in males than in females, few studies have clearly explained what causes this difference. The objective of the present study is to explain the sex difference in gingivitis based on the interaction between oral health behaviors and related factors, such as knowledge, attitude, and lifestyle, in young people.
    Methods: The study was comprised of 838 subjects (440 males and 398 females), aged 18 and 19 years. Gingivitis was assessed by the percentage of bleeding on probing (%BOP). Additional information was collected regarding oral hygiene status, oral health behaviors, and related factors. Structural equation modeling was used to test pathways from these factors to %BOP. Multiple-group modeling was also conducted to test for sex differences.
    Results: Females had greater knowledge, a more positive attitude, a healthier lifestyle, and higher level of oral health behaviors than males. There were significant differences in the paths (i.e., from lifestyle, knowledge, and attitude to %BOP) through oral health behaviors and oral health status.
    Conclusions: Sex-based differences in gingivitis in young people can be explained by oral health behaviors and hygiene status, which are influenced by lifestyle, knowledge, and attitude. To prevent gingivitis, different approaches to males and females may be useful. J Periodontol 2011;82:558-565.

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  • Occlusal disharmony induces BDNF level in rat submandibular gland. Reviewed International journal

    Koichiro Irie, Daisuke Ekuni, Takaaki Tomofuji, Tetsuji Azuma, Yasumasa Endo, Kenta Kasuyama, Manabu Morita

    Archives of oral biology   56 ( 1 )   35 - 40   2011.1

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    Objectives: Brain-derived neurotrophic factor (BDNF), which is produced in rat submandibular gland, is one of the most abundant neurotrophins in the central nervous system. It is generally accepted that occlusal disharmony causes stress. The purpose of the present study was to investigate whether occlusal disharmony-induced chronic stress affects BDNF levels and morphology in rat submandibular gland.
    Design: Eight wks old male Wistar rats (n = 21) were randomly divided into three groups of 7 rats. In a control (C) group, the rats received no treatment for 8 wks. In a molar cusp-less (OD) group, maxillary molar cusps were cut off with a dental turbine at baseline and kept for 8 wks. In a molar cusp-less + recovered cusp (OR) group, maxillary molar cusps were cut off and then were recovered after 4 wks using resin material. After the experimental period, expression of BDNF mRNA and protein as well as histological findings were evaluated in the submandibular glands. The comparisons between the groups were made using the Mann-Whitney U test with Bonferroni correction.
    Results: The OD group showed a significant increase in submandibular gland BDNF mRNA and protein expression after 8 wks, and plasma adrenocorticotropic hormone and corticosterone levels increased in a time-dependent manner. There were no significant differences in BDNF expression in the submandibular glands and in levels of plasma adrenocorticotropic hormone and corticosterone between the OR and C groups.
    Conclusions: These results indicate that psychological stress induced by occlusal disharmony reversibly induces BDNF expression in the rat submandibular gland. (C) 2010 Elsevier Ltd. All rights reserved.

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  • Relationship between decrease of erythrocyte count and progression of periodontal disease in a rural Japanese population. Reviewed International journal

    Tatsuo Yamamoto, Midori Tsuneishi, Michiko Furuta, Daisuke Ekuni, Manabu Morita, Yukio Hirata

    Journal of periodontology   82 ( 1 )   106 - 13   2011.1

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    BACKGROUND: Limited research has been reported on the relationship between periodontal status and erythrocyte parameters. In the present study, longitudinal data from health checkups are used to clarify the relationship between periodontal disease progression and changes in parameters of erythrocytes. METHODS: In this prospective study, a total of 120 subjects (35 men and 85 women; age range: 30 to 63 years) participated in a comprehensive health screening and dental checkup in 2006 and 2007. Medical examinations, including anthropometric and manometric measurements, blood-chemistry tests, and oral examinations, were performed. Subjects with periodontal disease progression (i.e., the progression group) were defined based on the presence of ≥1 tooth demonstrating a longitudinal loss of periodontal attachment ≥3 mm or tooth loss during the study period. The Wilcoxon signed-rank, χ(2), Fisher exact, and Mann-Whitney U tests and stepwise logistic regression analysis were used for statistical analyses. RESULTS: The progression group comprised 30 subjects. A significant difference between the progression and non-progression groups was observed in changes of erythrocyte counts but not those of the body mass index, systolic and diastolic blood pressure, creatinine, fasting blood glucose, hemoglobin, hematocrit, triglycerides, high- and low-density lipoprotein cholesterol, total cholesterol, aspartate and alanine aminotransferase, and γ-glutamyl transpeptidase. Stepwise logistic regression analysis showed that the progression of periodontal disease was associated with a change (year 2007 minus year 2006) of erythrocyte counts (adjusted odds ratio = 0.970; P = 0.009) after adjusting for age at baseline. CONCLUSION: Within the limitations of this study, the progression of periodontal disease is associated with a decrease in erythrocyte counts in a rural Japanese population.

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  • Observation of Internal Distribution Behavior of Micro/Nano-Sized Ceramics and Metal Particles in Mice Reviewed

    Shigeaki Abe, Nobuki Iwadera, Mami Mutoh, Chika Koyama, Mitsue Esaki, Tsukasa Akasaka, Motohiro Uo, Manabu Morita, Yoshinori Kuboki, Koichi Haneda, Yasutaka Yawaka, Fumio Uchida, Tetsu Yonezawa, Fumio Watari

    Bioceramics Development and Applications   1   D110134   2011.1

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    DOI: 10.4303/bda/D110134

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  • Supplementation of green tea catechins in dentifrices suppresses gingival oxidative stress and periodontal inflammation. Reviewed International journal

    Takayuki Maruyama, Takaaki Tomofuji, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Daisuke Ekuni, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    Archives of oral biology   56 ( 1 )   48 - 53   2011.1

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    OBJECTIVE: this study examined the effects of a dentifrice containing green tea catechins on gingival oxidative stress and periodontal inflammation using a rat model. DESIGN: twenty-four male Wister rats were randomly divided into four groups. The first group (Control group) received no treatment for 8 weeks. Periodontal inflammation was induced in the second group for 8 weeks. Periodontal inflammation was induced in the last two groups for 8 weeks and dentifrices with or without green tea catechins were topically applied to the gingival sulcus daily for 4 weeks prior to the end of the experimental period. RESULTS: rats that had experimental periodontal inflammation showed apical migration of the junctional epithelium, alveolar bone loss and inflammatory cell infiltration in the connective tissue subjacent to the junctional epithelium at 8 weeks, whilst the control group showed no pathologic changes. Topical application of a green tea catechin-containing dentifrice reduced inflammatory cell infiltration in the periodontal lesions to a greater degree than the control dentifrice at 8 weeks. The gingiva in which green tea catechin-containing dentifrice was applied also showed a lower level of expression of hexanoyl-lysine (a marker of lipid peroxidation), nitrotyrosine (a marker of oxidative protein damage), and tumour necrosis factor-α (an indicator of pro-inflammatory cytokines) at 8 weeks compared to gingiva in which the control dentifrice was applied. CONCLUSIONS: adding green tea catechins to a dentifrice may contribute to prevention of periodontal inflammation by decreasing gingival oxidative stress and expression of pro-inflammatory cytokines.

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  • Oral malodorous compound induces osteoclast differentiation without receptor activator of nuclear factor κB ligand. Reviewed International journal

    Hisataka Ii, Toshio Imai, Ken Yaegaki, Koichiro Irie, Daisuke Ekuni, Manabu Morita

    Journal of periodontology   81 ( 11 )   1691 - 7   2010.11

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    Background: Hydrogen sulfide (H(2)S), the main component of halitosis, is one of the etiologic factors for periodontitis. We recently reported that H(2)S may induce pathologic changes in rat alveolar bone. The objective of this study is to determine the effect of H(2)S on osteoclast differentiation.
    Methods: Murine macrophage cells RAW264 were cultured in medium lacking nuclear factor kappa B ligand (receptor activator of nuclear factor kappa B ligand) in 5% CO(2) with air at 37 degrees C for 24 hours; then 0.05, 0.5, or 5 ng/ml H(2)S was added to the CO(2)-air mix for 4 days. The controls received the CO(2)-air mix with no H(2)S. Cell differentiation was evaluated by counting the tartrate-resistant acid-phosphatase (TRAP) positive cells. Extracellular signaling-regulated kinase1/2 (ERK1/2) and mitogen-activated protein kinase p38 phosphorylation were examined by Western blotting. The bone-resorption activity was determined with the resorption assay of calcium phosphate.
    Results: There were significantly more TRAP-positive cells at a concentration of 0.05 ng/ml H(2)S than at the other concentrations (P &lt;0.001). Cathepsin K protein, a specific marker for osteoclasts, was expressed in the H(2)S-induced multinuclear cells. Resorption of calcium phosphate significantly increased in the H(2)S-induced TRAP-positive cells cultured on plates coated with calcium phosphate apatite (P &lt;0.01). The phosphorylation of ERK1/2 and p38 were accelerated by H(2)S, and increased with time. PD98059 and SB203580, specific inhibitors of ERK1/2 and p38, suppressed the activation of these enzymes and osteoclast differentiation by H(2)S.
    Conclusion: Results demonstrate that H(2)S at physiologic concentrations in mouth air induces osteoclasts from RAW264 cells. J Periodontol 2010;81:1691-1697.

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  • Relationship between periodontal condition and arterial properties in an adult population in Japan. Reviewed

    M Furuta, T Tomofuji, D Ekuni, Naofumi Tamaki, T Yamamoto, T Azuma, K Irie, Y Endo, S Yamada, M Morita

    Oral Diseases   Vol.16 ( No.8 )   781 - 787   2010.11

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    Oral Diseases (2010) 16, 781-787 Objective: This study addressed the relationship between periodontal condition and second derivative of the finger photoplethysmogram (SDPTG) in Japanese adults. Subject and methods: The Community Periodontal Index (CPI) and SDPTG were recorded in 415 subjects (mean age: 44.0 years). For assessing SDPTG, we mainly focused on the ratio of the absolute value of the height of the early negative &amp;apos;b&amp;apos; wave and ratio of the late re-decreasing &amp;apos;d&amp;apos; wave to the height of the initial positive &amp;apos;a&amp;apos; wave, namely the b/a and d/a ratios. Results: The CPI score was positively correlated with the b/a ratio (P &lt; 0.001), and negatively correlated with the d/a ratio (P &lt; 0.001). Logistic regression analysis showed that subjects with CPI scores ? 3 were more likely to have a higher level (male &gt; -0.69, female &gt; -0.64) of b/a ratio (Odds ratio = 1.7, P = 0.026) and lower level (male ? -0.29, female ? -0.32) of d/a ratio (Odds ratio = 2.2, P =0.001) than those with CPI scores 0-2, after adjusting for age, gender, smoking status, pulse rate and presence of hypertension. Conclusion: There was a statistical association between the CPI scores and SDPTG indices in Japanese adults.

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  • [Relationship between community-based dental health programs and health care costs for the metabolic syndrome]. Reviewed

    Noriko Takeuchi, Tatsuo Yamamoto, Aya Hirai, Manabu Morita, Ryousei Kodera

    [Nihon koshu eisei zasshi] Japanese journal of public health   57 ( 11 )   959 - 67   2010.11

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    OBJECTIVE: Health care costs have been increasing year by year and health programs are needed which will allow reduction in the burden. The present community-based ecological study examined the relationship between implementation of dental health care programs and health care costs for the metabolic syndrome. METHODS: We calculated the monthly health care cost for the metabolic syndrome per capita for each municipality in Okayama Prefecture (n = 27) using the national health insurance receipts for 1997 and 2007 for diabetes mellitus, hypertension, cardiovascular disorder, cerebral vascular disorder, and atherosclerosis as principal diseases. Information was obtained from each municipality on the implementation of public dental health services consisting of 10 programs, including visits for oral hygiene guidance, health consultation for periodontal disease, preventive long-term care, participation of dental hygienists in public health service, programs for improving oral function in the aged, and etc. The municipalities were divided into two groups based on the implementation/non-implementation of each dental health program. Then, the change in health care cost for metabolic syndrome per capita between 1997 and 2007 was compared between the two groups according to each dental health program. RESULTS Health care costs for metabolic syndrome were reduced in decade in the municipalities which executed dental health care programs such as 'preventive long-term care' or 'health consultation for periodontal disease', being greater in the municipalities which did not. More decrease in health care costs was further observed in the municipalities where the other seven programs were also implemented. DISCUSSION: Any direct relationship between dental health programs and health care costs for the metabolic syndrome remains unclear. However, our data suggests that costs might be decreased in municipalities which can afford to implement dental health programs. CONCLUSION: Health care costs for the metabolic syndrome in municipalities which executed dental health care programs tended to decrease in ten years.

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  • Supplementation of green tea catechins in dentifrices suppresses gingival oxidative stress and periodontal inflammation. Reviewed

    Takayuki Maruyama, Takaaki Tomofuji, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Daisuke Ekuni, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    Archives of Oral Biology   Vol.56 ( No.1 )   48 - 53   2010.9

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    adding green tea catechins to a dentifrice may contribute to prevention of periodontal inflammation by decreasing gingival oxidative stress and expression of pro-inflammatory cytokines.

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  • Internal distribution of micro-/nano-sized ceramics and metals particles in mice Reviewed

    Shigeki Abe, Ikuhiro Kida, Mitsue Esaki, Nobuki Iwadera, Mami Mutoh, Chika Koyama, Tsukasa Akasaka, Motohiro Uo, Yoshinori Kuboki, Manabu Morita, Yoshinori Sato, Koichi Haneda, Tetsu Yonezawa, Balachandran Jeyadevan, Kazuyuki Tohji, Fumio Watari

    Journal of the Ceramic Society of Japan   118   525 - 529   2010.6

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    DOI: 10.2109/jcersj2.118.525

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  • Experimental periodontitis induces gene expression of proinflammatory cytokines in liver and white adipose tissues in obesity. Reviewed International journal

    Yasumasa Endo, Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    Journal of periodontology   81 ( 4 )   520 - 6   2010.4

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    Systemic low-grade inflammation after experimental periodontitis was associated with increased gene expression for hepatic levels of TNF-alpha and CRP and adipose tissue levels of IL-6 and CRP in the obese-rat model.

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  • Effects of periodontitis on aortic insulin resistance in an obese rat model. Reviewed International journal

    Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Tetsuji Azuma, Naofumi Tamaki, Toshihiro Sanbe, Yasumasa Endo, Tatsuo Yamamoto, Takashi Nishida, Manabu Morita

    Laboratory investigation; a journal of technical methods and pathology   90 ( 3 )   348 - 59   2010.3

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    The combination of obesity and its associated risk factors, such as insulin resistance and inflammation, results in the development of atherosclerosis. However, the effects of periodontitis on atherosclerosis in an obese body remain unclear. The aim of the study was to investigate the effects of ligature-induced periodontitis in Zucker fatty rats on initiation of atherosclerosis by evaluating aortic insulin resistance. Zucker fatty rats (n=24) were divided into two groups. In the periodontitis group, periodontitis was ligature-induced for 4 weeks, whereas the control group was left unligated. After the 4-week experimental period, descending aorta was used for measuring the levels of lipid deposits, immunohistochemical analysis, and evaluation of gene expression. Levels of serum C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-alpha), and insulin were also measured. Rats in the periodontitis group had significantly enhanced lipid deposits in the aorta, but not in the control group. Expression of suppressor of cytokine signaling 3, vascular cell adhesion molecule 1, reactive oxygen species, nitrotyrosine, and endothelin-1 in the periodontitis group was more intense than that in the control group. Significantly decreased levels of phosphatidylinositol 3-kinase (Pi3k) catalytic beta-polypeptide (Pi3kcb), Pi3kp85, and insulin receptor substrate 1 and 2 were observed in the periodontitis group. Levels of serum CRP and TNF-alpha were significantly increased in the periodontitis group. Under insulin-stimulated conditions, aorta in the periodontitis group altered the Akt phosphorylation. Periodontitis in obesity induced the initial stage of atherosclerosis and disturbed aortic insulin signaling.

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  • Imbalance of oxidative/anti-oxidative status induced by periodontitis is involved in apoptosis of rat submandibular glands. Reviewed International journal

    Daisuke Ekuni, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takaaki Tomofuji, Manabu Morita

    Archives of oral biology   55 ( 2 )   170 - 6   2010.2

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    Imbalance of circulating oxidative/anti-oxidative status may be involved in vacuolisation and apoptosis of submandibular glands in the rat periodontitis model.

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  • Application of self-efficacy theory in dental clinical practice. Reviewed

    Kakudate N, Morita M, Fukuhara S, Sugai M, Nagayama M, Kawanami M, Chiba I

    Oral Diseases   16   747 - 752   2010

  • Preventive effects of a cocoa-enriched diet on gingival oxidative stress in experimental periodontitis. Reviewed International journal

    Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Yasumasa Endo, Naofumi Tamaki, Toshihiro Sanbe, Jun Murakami, Tatsuo Yamamoto, Manabu Morita

    Journal of periodontology   80 ( 11 )   1799 - 808   2009.11

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    Consuming a cocoa-enriched diet could diminish periodontitis-induced oxidative stress, which, in turn, might suppress the progression of periodontitis.

    DOI: 10.1902/jop.2009.090270

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  • A pilot study of a simple screening technique for estimation of salivary flow. International journal

    Takashi Kanehira, Tomotaka Yamaguchi, Junji Takehara, Haruhiko Kashiwazaki, Takae Abe, Manabu Morita, Kouzo Asano, Yoshinori Fujii, Wataru Sakamoto

    Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics   108 ( 3 )   389 - 93   2009.9

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    OBJECTIVES: The purpose of this study was to develop a simple screening technique for estimation of salivary flow and to test the usefulness of the method for determining decreased salivary flow. STUDY DESIGN: A novel assay system comprising 3 spots containing 30 microg starch and 49.6 microg potassium iodide per spot on filter paper and a coloring reagent, based on the color reaction of iodine-starch and theory of paper chromatography, was designed. We investigated the relationship between resting whole salivary rates and the number of colored spots on the filter produced by 41 hospitalized subjects. RESULTS: A significant negative correlation was observed between the number of colored spots and the resting salivary flow rate (n = 41; r = -0.803; P < .01). For all complaints of decreased salivary flow (n = 9) having cutoff values <100 microL/min for the salivary flow rate, 3 colored spots appeared on the paper, whereas for healthy subjects there was < or =1 colored spot. CONCLUSION: This novel assay system might be effective for estimation of salivary flow not only in healthy but also in bedridden and disabled elderly people.

    DOI: 10.1016/j.tripleo.2009.04.019

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  • [Study of a CT scanning method that improves gravity effects to enable differentiation of inflammatory diseases in chest CT].

    Hitoshi Fujita, Manabu Morita, Masayasu Kusaka

    Nihon Hoshasen Gijutsu Gakkai zasshi   64 ( 6 )   707 - 13   2008.6

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    In chest CT images, the dorsal lower lung field often shows an infiltration-like shadow in patients who cannot stop breathing or take a deep breath. The cause of this phenomenon might be due to the effects of gravity. Since we had observed decreased effects of gravity by conducting additional CT scanning for patients in an oblique position (55 degrees ) or a nearly lateral position, we conducted a clinical study to investigate this matter. Forty-three patients (23 patients in the normal group and 20 patients in the inflammatory disease group) who underwent additional CT scanning were included in this study. CT values for the region in which infiltration-like shadow was observed in both positions (dorsal position and oblique position) were measured. The ratio of fluctuation in the CT value of the dorsal lower lung field at a positional change from the dorsal to the oblique position was calculated as a coefficient of fluctuation C (%). As a result, the coefficient of fluctuation C (%) was 32.6+/-13.6 in the normal group and 6.7+/-6.8 in the inflammatory disease group. The effects of gravity were improved by additional CT scanning in an oblique position (55 degrees ) or a nearly lateral position, and this enabled differentiation of the effects of gravity vs. inflammatory diseases.

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  • Identification of new amine acceptor protein substrate candidates of transglutaminase in rat liver extract: use of 5-(biotinamido) pentylamine as a probe. International journal

    Akira Ichikawa, Jin Ishizaki, Manabu Morita, Kentaro Tanaka, Koji Ikura

    Bioscience, biotechnology, and biochemistry   72 ( 4 )   1056 - 62   2008.4

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    Transglutaminases (TGs) are a family of enzymes that catalyze Ca(2+)-dependent post-translational modification of proteins by introducing protein-protein crosslinks (between specific glutamine and lysine residues), amine incorporation, and site-specific deamidation. In this study, new amine acceptor protein substrates of TG were isolated from rat liver extract and identified using 5-(biotinamido) pentylamine, a biotinylated primary amine substrate, as a probe. TG protein substrate candidates labeled with biotin by endogenous TG activity were isolated and recovered by avidin column chromatography. Proteins with molecular masses of 40, 42, and 45 kDa were the main components of the labeled proteins. Determination of their partial amino acid sequences and immunoblotting analyses were done to identify them. The 45-kDa protein was identical with betaine-homocysteine S-methyltransferase (EC 2.2.2.5), which was identified in our previous study. The 40- and 42-kDa proteins were identified as arginase-I (EC 3.5.3.1) and fructose-1,6-bisphosphatase (EC 3.1.3.11) respectively. TG catalyzed incorporation of 5-(biotinamido) pentylamine into both arginase-I and fructose-1,6-bisphosphatase purified from rat liver was confirmed in vitro. These results suggest that these two enzymes are the new protein substrate candidates of TG and that they can be modified post-translationally by cellular TG.

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  • 経験的ベイズ推定値を用いた市町村別3歳児う蝕有病者率の地域比較および歯科保健水準との関連

    相田 潤, 安藤 雄一, 青山 旬, 丹後 俊郎, 森田 学

    口腔衛生学会雑誌   54 ( 5 )   566 - 576   2004.10

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    1998年(3122市町村)と2000年(全3244市町村)における3歳児歯科健康診査結果を基に齲蝕有病率の経験的ベイズ推定値による疾病地図を作成して地域比較を行った.さらに重回帰分析により歯科保健水準に関する指標について社会背景指標を調整して検討した.その結果,疾病地図では北海道・東北・四国・九州を中心に有病率が高い傾向が認められ,重回帰分析では「最終学歴割合(大学)」「合計特殊出産率」「二次産業従業者割合」「失業率」の指標との関連が強く,「保健指導受診回数」が齲蝕を減少させる方向に弱く関連していた.以上より,経験的ベイズ推定値の疾病地図により3歳児齲蝕有病率の市町村単位の地域差が明確となり,「保健指導受診回数」の高い地域で有病率が低い傾向が弱いながら認められたことから本研究結果は二次資料を利用した地域診断の第一歩として活用できるものと考えられた

    DOI: 10.5834/jdh.54.5_566

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  • Relationship between blood triglyceride levels and periodontal status. International journal

    Manabu Morita, Masazumi Horiuchi, Yuka Kinoshita, Tatsuo Yamamoto, Tatsuo Watanabe

    Community dental health   21 ( 1 )   32 - 6   2004.3

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    OBJECTIVES: The literature suggests that a link exists between the presence of periodontal disease and impaired lipid metabolism. However, most studies have focused on patients in private clinics or university hospitals. In the present study, we assessed associations between blood chemistry variables and periodontal disease status in rural communities of Japan. METHODS: A total of 823 residents participated in a comprehensive health screen. The medical screen included measurement of body mass index, blood pressure, hematocrit and blood chemistry, including levels of hemoglobin, total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglycerides, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), gamma-glutamyl transpeptidase (gamma-GTP), creatinine, and plasma glucose. Periodontal status was assessed using the CPI scoring method. Of those participating, 133 subjects with a CPI of 4 (periodontal disease group) and age- and gender-matched control subjects with a CPI of less than 3 were selected for analysis. RESULTS: The mean triglyceride level was significantly higher for the diseased group than for the control group (p < 0.05). The mean HDL-C level was higher for the control group than for the diseased group, although the difference was not statistically significant. Logistic regression model analysis revealed a significant relationship between elevated triglycerides (> 149 mg/dl) and the presence of periodontal disease (OR = 2.26 vs. < or = 149 mg/dl, p = 0.014). CONCLUSION: The results indicate that serum triglyceride level might be a potential indicator for the presence of periodontal disease, suggesting the need for community-based longitudinal studies.

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  • Relationship between structures and biological activities of mycoplasmal diacylated lipopeptides and their recognition by toll-like receptors 2 and 6. International journal

    Tsugumi Okusawa, Mari Fujita, Jun-Ichiro Nakamura, Takeshi Into, Motoaki Yasuda, Atsutoshi Yoshimura, Yoshitaka Hara, Akira Hasebe, Douglas T Golenbock, Manabu Morita, Yoshio Kuroki, Tomohiko Ogawa, Ken-Ichiro Shibata

    Infection and immunity   72 ( 3 )   1657 - 65   2004.3

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    The lipopeptide FSL-1 [S-(2,3-bispalmitoyloxypropyl)-Cys-Gly-Asp-Pro-Lys-His-Pro-Lys-Ser-Phe, Pam(2)CGDPKHPKSF] synthesized on the basis of the N-terminal structure of a Mycoplasma salivarium lipoprotein capable of activating normal human gingival fibroblasts to induce the cell surface expression of ICAM-1 revealed an activity to induce production of monocyte chemoattractant protein 1, interleukin-6 (IL-6), and IL-8. FSL-1 also activated macrophages to produce tumor necrosis factor alpha as the Mycoplasma fermentans-derived lipopeptide MALP-2 (Pam(2)CGNNDESNISFKEK), a potent macrophage-activating lipopeptide, did. The level of the activity of FSL-1 was higher than that of MALP-2. This result suggests that the difference in the amino acid sequence of the peptide portion affects the activity because the framework structure other than the amino acid sequence of the former is the same as that of the latter. To determine minimal structural requirements for the activity of FSL-1, the diacylglyceryl Cys and the peptide portions were examined for this activity. Both portions did not reveal the activity. A single amino acid substitution from Phe to Arg and a fatty acid substitution from palmitic acid to stearic acid drastically reduced the activity. Similar results were obtained in measuring the NF-kappaB reporter activity of FSL-1 to human embryonic kidney 293 cells transfected with Toll-like receptor 2 and 6, together with a NF-kappaB-dependent luciferase reporter plasmid. These results suggest that both the diacylglyceryl and the peptide portions of FSL-1 are indispensable for the expression of biological activities and for the recognition by Toll-like receptors 2 and 6 and that the recognition of FSL-1 by Toll-like receptors 2 and 6 appears to be hydrophobic.

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  • Prevalence of oral malodor and the relationship with habitual mouth breathing in children. International journal

    Takashi Kanehira, Junji Takehara, Dairo Takahashi, Okahito Honda, Manabu Morita

    The Journal of clinical pediatric dentistry   28 ( 4 )   285 - 8   2004

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    The prevalence of oral malodor and association of habitual mouth breathing with oral malodor were investigated in children residing in rural areas. One hundred and nineteen children participated in this study. A sulfide monitor and organoleptic method were used to evaluate oral malodor. About 8% of children had a sulfide level in mouth air above the socially acceptable limit (75 ppb). Habitual mouth breathing was a factor contributing to oral malodor. Oral malodor was not significantly correlated with plaque index, history of caries or frequency of toothbrushing.

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  • Discrimination between composite resin and teeth using fluorescence properties.

    Kazutoshi Tani, Fumio Watari, Motohiro Uo, Manabu Morita

    Dental materials journal   22 ( 4 )   569 - 80   2003.12

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    The differentiation of composite resin from teeth using fluorescence emission was investigated as basic research for the visual detection of resin filled teeth in mass dental health examinations. Fluorescence spectra were taken from extracted human maxillary central incisors and 12 types of light-cured composite resins with a maximum of 15 shades via excitation using light with wavelengths of 400-500 nm. The fluorescence intensity ratio of resin to tooth was lowest around 500 nm for all the resins. The fluorescent images were taken based on spectroscopic results, which confirmed discrimination between the resin part and the tooth in the resin filled tooth.

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  • Involvement of leucine residues at positions 107, 112, and 115 in a leucine-rich repeat motif of human Toll-like receptor 2 in the recognition of diacylated lipoproteins and lipopeptides and Staphylococcus aureus peptidoglycans. International journal

    Mari Fujita, Takeshi Into, Motoaki Yasuda, Tsugumi Okusawa, Sumiko Hamahira, Yoshio Kuroki, Akiko Eto, Toshiki Nisizawa, Manabu Morita, Ken-ichiro Shibata

    Journal of immunology (Baltimore, Md. : 1950)   171 ( 7 )   3675 - 83   2003.10

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    S-(2,3-bispalmitoyloxypropyl)Cys-Gly-Asp-Pro-Lys-His-Pro-Lys-Ser-Phe (FSL-1) derived from Mycoplasma salivarium stimulated NF-kappaB reporter activity in human embryonic kidney 293 (HEK293) cells transfected with Toll-like receptor 2 (TLR2) or cotransfected with TLR2 and TLR6, but not in HEK293 cells transfected with TLR6, in a dose-dependent manner. The activity was significantly higher in HEK293 cells transfected with both TLR2 and TLR6 than in HEK293 cells transfected with only TLR2. The deletion mutant TLR2(DeltaS40-I64) (a TLR2 mutant with a deletion of the region of Ser(40) to Ile(64)) failed to activate NF-kappaB in response to FSL-1. The deletion mutant TLR2(DeltaC30-S39) induced NF-kappaB reporter activity, but the level of activity was significantly reduced compared with that induced by wild-type TLR2. A TLR2 point mutant with a substitution of Glu(178) to Ala (TLR2(E178A)), TLR2(E180A), TLR2(E190A), and TLR2(L132E) induced NF-kappaB activation when stimulated with FSL-1, M. salivarium lipoproteins, and Staphylococcus aureus peptidoglycans, but TLR2(L107E), TLR2(L112E) (a TLR2 point mutant with a substitution of Leu(112) to Glu), and TLR2(L115E) failed to induce NF-kappaB activation, suggesting that these residues are essential for their signaling. Flow cytometric analysis demonstrated that TLR2(L115E), TLR2(L112E), and TLR2(DeltaS40-I64) were expressed on the cell surface of the transfectants as wild-type TLR2 and TLR2(E190A) were. In addition, these mutants, except for TLR2(E180A), functioned as dominant negative form of TLR2. This study strongly suggested that the extracellular region of Ser(40)-Ile(64) and leucine residues at positions 107, 112, and 115 in a leucine-rich repeat motif of TLR2 are involved in the recognition of mycoplasmal diacylated lipoproteins and lipopeptides and in the recognition of S. aureus peptidoglycans.

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  • Effect of initial periodontal therapy on sulcular/tongue sulfide level. A pilot study. International journal

    Manabu Morita, Hom-Lay Wang

    Journal of clinical periodontology   29 ( 9 )   844 - 7   2002.9

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    BACKGROUND: : Volatile sulfur compounds (VSC), such as hydrogen sulfide, methyl mercaptan and dimethyl sulfide, are toxic metabolites produced by periodontal pathogens. These VSC are also released from the tongue dorsum. Currently, limited data are available on how periodontal treatments may affect sulcular/tongue sulfide level. The aim of this study was to determine effect of initial therapy including oral hygiene instruction and scaling and root planing (SRP) on sulcular/tongue sulfide level. METHODS: : Thirteen subjects diagnosed with chronic periodontitis and having three representative periodontal pocket depth (PD) strata in one quadrant (PD <or= 3.0 mm: healthy, 4.0 mm <or= PD <or= 6.0 mm: moderate, and PD >or= 7.0 mm: severe disease sites) were selected. Clinical periodontal parameters were recorded at baseline and 3 weeks after completion of the initial therapy. The sulfide levels in three representative periodontal pockets (pS) and on three parts (anterior, middle, and posterior) of the tongue dorsum (tS) were measured using a commercial sulfide-monitoring device. RESULTS: : The pS levels of the three representative sites were reduced significantly following the initial therapy (p < 0.05). However, there was no significant reduction of the tS level in all three parts of the tongue. CONCLUSIONS: : The initial periodontal therapy such as oral hygiene and SRP reduces the sulcular sulfide level but not the tongue sulfide level. This suggests that sulcular sulfide level may be a possible indicator for assessing the outcome of initial periodontal treatment.

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  • Synergic effects of mycoplasmal lipopeptides and extracellular ATP on activation of macrophages. International journal

    Takeshi Into, Mari Fujita, Tsugumi Okusawa, Akira Hasebe, Manabu Morita, Ken-Ichiro Shibata

    Infection and immunity   70 ( 7 )   3586 - 91   2002.7

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    Mycoplasmal lipopeptides S-(2,3-bispalmitoyloxypropyl)-CGDPKHSPKSF and S-(2,3-bispalmitoyloxypropyl)-CGNNDESNISFKEK activated a monocytic cell line, THP-1 cells, to produce tumor necrosis factor alpha. The activity of the lipopeptides was augmented by ATP in a dose-dependent manner. In addition, the level of expression of mRNAs for tumor necrosis factor alpha and interleukin-1 beta, -6, and -8 was also upregulated by the lipopeptides and/or extracellular ATP, but that of interleukin-10 was not. The P2X purinergic receptor antagonists pyridoxal phosphate 6-azophenyl 2',4'-disulfonic acid and periodate-oxidized ATP suppressed the activity of ATP to augment the activation of THP-1 cells by the lipopeptides, suggesting that P2X receptors play important roles in the activity of ATP. The nuclear factor kappa B inhibitor dexamethasone also suppressed the activity, suggesting that the activity of ATP is dependent upon the nuclear factor kappa B. Thus, these results suggest that the interaction of extracellular ATP with the P2X receptors is attributed to the activity of ATP to augment the activation of THP-1 cells by mycoplasmal lipopeptides.

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Books

  • 新編 衛生学・公衆衛生学

    森田 学( Role: Joint author ,  健康食品 廃棄物処理 放射線と健康)

    医歯薬出版株式会社  2021.3 

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  • 今日の診断指針 第8版

    森田 学(口臭)

    医学書院  2020.3 

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  • New 予防医学・公衆衛生学

    森田 学( Role: Sole author ,  口腔保健)

    南江堂  2018.11 

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  • ビジュアル歯周病を科学する

    森田 学( Role: Sole author ,  歯周病の有病率とリスクファクター)

    2012.5 

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  • Development in Higher Education

    Manabu Morita, Naoki Kakudate, Itsuo Chiba, Masamitu Kawanami( Role: Joint author)

    Nova Science Publishers, Inc.  2010.10 

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  • 口腔保健学

    医歯薬出版株式会社  2009 

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  • 新予防歯科学

    医歯薬出版,東京  2008 

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  • 口腔衛生学 -口腔保健統計を含む-

    学建書院,東京  2008 

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  • 衛生学・公衆衛生学

    医歯薬出版,東京  2008 

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  • 衛生学・公衆衛生学

    医歯薬出版  2008 

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  • NEW予防医学・公衆衛生学(第2版)

    南江堂  2006 

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  • 歯科医療 人間科学へのいざない

    医歯薬出版  2005 

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MISC

  • 唾液中乳酸脱水素酵素(LDH)測定用簡易キットによるスコアの悪化にはセルフエフィカシーが関係する

    江國 大輔, 片岡 広太, 福原 大樹, 内田 瑶子, 外山 直樹, 小林 暉政, イスラム・モハマド・モニルル, 澤田 ななみ, 森田 学

    日本歯科医師会雑誌   74 ( 4 )   407 - 407   2021.7

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    Language:Japanese   Publisher:(公社)日本歯科医師会  

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  • チェアサイドQ&A 予防歯科の疑問[第43回] 洗口液の効果的な使用のタイミングが知りたいです(Q&A)

    森田 学

    歯科衛生士   45 ( 7 )   21 - 21   2021.7

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  • 口腔内局所臭気回収方法の最適化に関する研究

    松本 卓也, 高橋 有弥瑠, 松尾 明彦, 江國 大輔, 森田 学

    日本口臭学会会誌   12 ( 1 )   46 - 46   2021.5

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  • 「口臭による日常生活への支障」に関連する要因

    佐保 輝, 横井 彩, 丸山 貴之, 竹内 倫子, 江國 大輔, 森田 学

    日本口臭学会会誌   12 ( 1 )   49 - 50   2021.5

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  • ヘルスリテラシーとリスク選好が定期的な歯科健診への受診意志に与える影響

    角田 伊知郎, 森田 学, 江國 大輔, 丸山 貴之, 片岡 広太, 横井 彩, 福原 大樹, 福原 瑶子, 外山 直樹, 中原 桃子

    口腔衛生学会雑誌   71 ( 増刊 )   88 - 88   2021.4

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  • 頭頸部がん治療による口腔機能・QOLへの影響 縦断研究

    横井 彩, 丸山 貴之, 中原 桃子, 山中 玲子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   71 ( 増刊 )   116 - 116   2021.4

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  • インドネシア共和国バリ島在住邦人からメールによる歯科相談を受けた下顎骨周囲炎の1例

    原田 祥二, 藤田 真理, 池田 美子, 菅野 真人, 江端 正祐, 兼平 孝, 森田 学

    口腔衛生学会雑誌   71 ( 増刊 )   92 - 92   2021.4

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  • 岡山大学病院頭頸部がんセンターにおける頭頸部がん支持療法に関する臨床実習教育の取り組みについて

    丸山 貴之, 横井 彩, 中原 桃子, 佐々木 禎子, 梶谷 明子, 藤代 万由, 中本 美保, 吉田 陽子, 森田 学, 三浦 留美, 山中 玲子, 水川 展吉, 浅海 淳一, 安藤 瑞生, 木股 敬裕

    日本口腔ケア学会雑誌   15 ( 3 )   186 - 186   2021.4

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  • Effects of diabetes mellitus and periodontitis on Alzheimer disease

    Islam Md Monirul, Daisuke Ekuni, Kota Kataoka, Daiki Fukuhara, Yoko Uchida-Fukuhara, Momoko Nakahara, Manabu Morita

    71(増刊)   94 - 94   2021.4

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  • 昼間の養育別にみた三歳児のう蝕の罹患状況の推移について

    河本 幸子, 横井 彩, 森田 学

    口腔衛生学会雑誌   71 ( 増刊 )   98 - 98   2021.4

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  • 地域歯科口腔保健の現状と課題 公衆衛生モニタリング・レポート委員会口腔保健分野 活動総括

    三浦 宏子, 福田 英輝, 加藤 一夫, 竹内 倫子, 渡邉 功, 小島 美樹, 上林 宏次, 芝田 登美子, 田野 ルミ, 玉置 洋, 橋本 由利子, 埴岡 隆, 村松 真澄, 本橋 佳子, 森田 学, 尾崎 哲則

    日本公衆衛生雑誌   68 ( 2 )   83 - 91   2021.2

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    目的 公衆衛生モニタリング・レポート委員会口腔保健分野での2015年以降のモニタリング活動で得られた成果・知見を体系的に整理し、今後の歯科保健活動の推進に資する資料として提示する。方法 公衆衛生モニタリング・レポート委員会口腔保健分野のグループ活動の年次報告内容を中核として、2015年以降のライフステージ別の地域歯科保健情報を整理するとともに、分野横断的課題として市販製剤を活用した歯科保健と医科歯科連携に基づくたばこ対策の動向について文献等を追加収集して分析した。結果 乳幼児・小児期における「う蝕」の有病状況は低減傾向にあった。その一方で、わが国の小児における歯・口腔の健康格差に対する具体的な対策に関しては、フッ化物応用以外はいまだ十分なエビデンスは報告されていなかった。成人期・高齢期の課題である「歯周病予防」「オーラルフレイル予防」「要介護高齢者の口腔管理」のいずれにおいても、そのニーズは一貫して増加していた。歯周病は生活習慣病のリスク要因のひとつであり、喫煙とも密接な関連性を有する。しかし、地域保健における歯周病対策は十分ではなく、有病状況について改善されていなかった。超高齢社会の新しい概念であるオーラルフレイルは、2017年以降に学術報告数が急増し、社会での周知が急速に進んだ。在宅要介護高齢者への口腔管理は、ニーズに見合った提供が十分なされておらず、地域包括ケア推進の観点からも更なる改善が求められる。効果が高い新規の歯科予防製剤の動向は、歯科におけるセルフケアの向上に大きく寄与する。高濃度フッ化物配合歯磨剤は、医薬部外品として広く市中で販売されるようになるとともに、フッ化物洗口剤も2019年9月に第3類医薬品に移行した。一方、歯科からアプローチするたばこ対策の有用性はWHO報告書でも指摘されたが、「たばこ対策」に取り組んでいる歯科診療所はいまだ十分ではなかった。結論 地域歯科口腔保健に関連する課題はライフステージの影響を受けるため多様であるが、その課題解決のためには、歯科専門職以外の関連職種との連携が不可欠なものが多かった。公衆衛生モニタリング・レポート委員会での口腔保健グループの活動においても、さらに分野横断的なモニタリング活動が必要であることが示唆された。(著者抄録)

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  • 若年者と高齢者との間での口腔機能の比較

    澤田 ななみ, 竹内 倫子, 江國 大輔, 森田 学

    日本老年歯科医学会総会・学術大会プログラム・抄録集   31回   130 - 130   2020.11

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  • 基本チェックリストの口腔機能3項目と口腔機能との関連

    竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

    日本老年歯科医学会総会・学術大会プログラム・抄録集   31回   102 - 102   2020.11

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  • 歯周病最大のリスクファクターは"人"である 患者の思いを本当に理解していますか?

    森田 学

    DENTAL DIAMOND   45 ( 13 )   62 - 67   2020.10

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  • 1歳6ヵ月児および2歳6ヵ月児におけるう蝕発生要因の比較

    竹内 倫子, 江國 大輔, 外山 直樹, 森田 学

    日本公衆衛生学会総会抄録集   79回   367 - 367   2020.10

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  • 頭頸部がん治療による口腔機能・QOLへの影響:横断研究

    横井 彩, 丸山貴之, 小林暉政, 山中玲子, 江國大輔, 森田 学

    近畿・中国・四国口腔衛生学会 抄録集   2020.10

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  • 大学生における家庭での受動喫煙曝露とう蝕との関係:横断研究

    佐保 輝, 江國大輔, 横井 彩, 片岡広太, 福原大樹, 外山直樹, モニルルイスラム, 澤田ななみ, 中島千穂, 中原桃子, 出口純也, 福原(内田)瑶子, 米田俊樹, 森田 学

    近畿・中国・四国口腔衛生学会 抄録集   2020.10

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  • チェアサイドQ&A 予防歯科の疑問[第31回] チーズにはう蝕予防効果がある?

    森田 学

    歯科衛生士   44 ( 7 )   21 - 21   2020.7

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  • つまようじ法で口臭は変わるか?

    津野敬一郎, 渋田大路, 西村啓司, 山口一朗, 渡邊 豊, 森田 学

    日本口臭学会 抄録集   2020.6

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  • 歯周病試験紙を応用した職域における歯科検診の試み

    山本 龍生, 渕田 慎也, 持田 悠貴, 江國 大輔, 森田 学, 恒石 美登里

    産業衛生学雑誌   62 ( 臨増 )   406 - 406   2020.5

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  • 歯周病原性以外の口腔微生物への喫煙の影響 文献レビュー

    埴岡 隆, 内藤 麻利江, 谷口 奈央, 重石 英生, 杉山 勝, 森田 学, 竹下 徹, 花田 信弘, 小島 美樹, 小川 祐司, 山本 龍生

    口腔衛生学会雑誌   70 ( 増刊 )   165 - 165   2020.3

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  • 大学生における睡眠の質とストレスが歯周病に及ぼす影響(Influence of sleep quality and stress on periodontal disease among university students)

    Islam Monirul, Ekuni Daisuke, Toyama Naoki, Tabata Ayano, Kataoka Kota, Uchida Yoko, Fukuhara Daiki, Saho Hikari, Sawada Nanami, Nakashima Yukiho, Morita Manabu

    口腔衛生学会雑誌   70 ( 増刊 )   159 - 159   2020.3

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  • 大学入学後の居住形態の変化と歯周病発症との関連(前向きコホート研究) Reviewed

    中原 桃子, 田畑 綾乃, 外山 直樹, 米田 俊樹, 片岡 広太, 佐保 輝, Islam Monirul, 横井 彩, 江國 大輔, 森田 学

    口腔衛生学会雑誌   70 ( 増刊 )   159 - 159   2020.3

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  • 慢性歯周炎患者の歯周状態の悪化に関連する唾液中miRNAの探索 コホート研究

    藤森 浩平, 米田 俊樹, 杉浦 嘉雄, 丸山 貴之, 江國 大輔, 東 哲司, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   70 ( 増刊 )   158 - 158   2020.3

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  • 日中の咬筋の筋活動は歯周炎の進行と関係する:コホート研究

    江國大輔、加藤信也、川上滋央、丸山貴之、片岡広太、内田瑤子、福原大樹、外山直樹、皆木省吾、森田 学

    日本疫学会 抄録集   2020.2

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  • 大学生における歯科に関する知識、歯科に関する知識の情報源及び口腔保健行動との関連:前向きコホート研究

    福原 大樹, 江國 大輔, 片岡 広太, 田畑 綾乃, 内田 瑶子, 外山 直樹, 米田 俊樹, 杉浦 嘉雄, イスラム・モハマド・モニルル, 佐保 輝, 森田 学

    岡山歯学会雑誌   38 ( 2 )   93 - 93   2019.12

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  • 妊婦・パートナー歯科健康診査受診者の歯科保健行動について

    河本 幸子, 光藤 彩, 白神 瑞絵, 松岡 宏明, 竹内 倫子, 森田 学

    日本公衆衛生学会総会抄録集   78回   474 - 474   2019.10

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  • ダウン症候群と非ダウン症候群知的能力障害者との口腔状態の違いについて

    森 貴幸, 山本 龍生, 森田 学

    日本公衆衛生学会総会抄録集   78回   472 - 472   2019.10

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  • チェアサイドQ&A 予防歯科の疑問[第22回] 歯肉マッサージの臨床的効果は?

    森田 学

    歯科衛生士   43 ( 10 )   31 - 31   2019.10

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  • 舌口唇運動機能は身体フレイルに関連する

    竹内 倫子, 江國 大輔, 田畑 綾乃, 外山 直樹, 森田 学

    日本公衆衛生学会総会抄録集   78回   231 - 231   2019.10

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  • Influence of occupational stress and coping style on periodontitis among the Japanese workers: A cross-sectional study

    Islam Md M, Ekuni D, Yoneda T, Yokoi A, Morita M

    2019.9

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  • 若年者における受動喫煙、睡眠の質、および睡眠時ブラキシズムの関連:横断研究

    外山直樹, 江國大輔, 田畑綾乃, 米田俊樹, 片岡広太, 横井 彩, 内田瑶子, 福原大樹, 佐保 輝, イスラム モハマド モニルル, 澤田ななみ, 中島千穂, 森田 学

    近畿・中国・四国口腔衛生学会 抄録集   2019.9

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  • 分子標的薬併用放射線療法(BRT)中の頭頸部がん患者に対する口腔衛生管理を行った症例

    藤代 万由, 小林 暉政, 松崎 久美子, 佐々木 禎子, 梶谷 明子, 志茂 加代子, 中本 美保, 三浦 留美, 水川 展吉, 森田 学

    日本歯科衛生学会雑誌   14 ( 1 )   181 - 181   2019.8

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  • 医療トレンド 舌苔 Tongue coating

    森田 学

    Schneller   ( 111 )   17 - 21   2019.7

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  • いつまでも健口生活 オーラルフレイル

    森田 学

    さかえ: 月刊糖尿病ライフ   59 ( 5 )   50 - 51   2019.5

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  • 乳歯の発育障害の発現頻度と性別・出生体重・妊娠期間との関連について

    河本 幸子, 森田 学

    口腔衛生学会雑誌   69 ( 増刊 )   206 - 206   2019.4

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  • 歯周病ラットにおける血清miRNAの肝臓mRNAへの影響

    杉浦 嘉雄, 米田 俊樹, 藤森 浩平, 丸山 貴之, 宮井 久敬, 小林 暉政, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   69 ( 増刊 )   182 - 182   2019.4

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  • いつまでも健口生活 糖尿病患者さんへの歯科治療の効果

    森田 学

    さかえ: 月刊糖尿病ライフ   59 ( 4 )   24 - 25   2019.4

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  • K刑務所女子受刑者に対する歯科保健活動

    黒瀬 真由美, 山本 美由希, 岩崎 美幸, 村田 英利子, 堀田 佳美, 藤田 あゆみ, 長浦 寛子, 森田 学

    口腔衛生学会雑誌   69 ( 増刊 )   208 - 208   2019.4

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  • 高校生のDMF増加に関連する要因

    竹内 倫子, 澤田 ななみ, 田畑 綾乃, 森田 学

    口腔衛生学会雑誌   69 ( 増刊 )   205 - 205   2019.4

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  • 幼児における保育環境とう蝕発生との関係

    横井 彩, 竹内 倫子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   69 ( 増刊 )   205 - 205   2019.4

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  • いつまでも健口生活 咀嚼と肥満

    森田 学

    さかえ: 月刊糖尿病ライフ   59 ( 3 )   24 - 25   2019.3

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  • いつまでも健口生活 お口のニオイにご注意を

    森田 学

    さかえ: 月刊糖尿病ライフ   59 ( 2 )   24 - 25   2019.2

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  • 「WHO簡易タバコ介入法の日本の歯科医療への統合」の普及持続のための統合ASP利用eラーニングシステム開発

    小島美樹, 埴岡 隆, 谷口奈央, 廣藤卓雄, 小川祐司, 田野ルミ, 川口陽子, 千葉逸朗, 森田 学

    日本禁煙医師歯科医師連盟総会・学術総会 抄録集   2019.2

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  • 「WHO簡易タバコ介入法の日本の歯科医療への統合」事業9か月間のまとめと課題

    埴岡 隆, 谷口奈央, 廣藤卓雄, 川口陽子, 千葉逸朗, 小島美樹, 小川祐司, 田野ルミ, 森田 学

    日本禁煙医師歯科医師連盟総会・学術総会 抄録集   2019.2

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  • いつまでも健口生活 清掃用具あれこれ

    森田 学

    さかえ: 月刊糖尿病ライフ   59 ( 1 )   24 - 25   2019.1

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  • ラット歯周病モデルにおける血清miRNAと肝臓への影響

    杉浦 嘉雄, 米田 俊樹, 藤森 浩平, 丸山 貴之, 宮井 久敬, 小林 暉政, 江國 大輔, 友藤 孝明, 森田 学

    岡山歯学会雑誌   37 ( 2 )   81 - 81   2018.12

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  • スペシャルニーズ歯科女性患者における骨密度変化と歯周組織の状態変化との関連に関する研究

    森 貴幸, 野島 靖子, 関 愛子, 村田 尚道, 山本 昌直, 田尻 絢子, 東 倫子, 前川 享子, 高石 芽求, 山本 龍生, 森田 学, 江草 正彦

    岡山歯学会雑誌   37 ( 2 )   61 - 70   2018.12

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    スペシャルニーズ歯科受診患者において、歯周病の進行が全身の骨密度低下の予測因子となり得るか評価した。知的能力障害(MR)、ダウン症候群(DS)または脳性麻痺等の身体障害(PD)のいずれかを有し、骨密度測定が可能であった23名を対象とした。骨梁面積率の変化は、観察期間中に増加した者10名、減少した者13名であり、障害群別に有意差は認めなかった。歯周病原因での歯の喪失またはペリオドンタルインデックス値の有意な増大で定義した歯周病進行群に当てはまる者は9名(39.1%)で、非進行群は14名(60.9%)であった。進行群9名の障害別内訳はMR群4名、DS群4名、PD群1名であった。歯周病進行群の骨梁面積率変化の平均値は-2.0±8.93%、非進行群は0.93±2.84%であり、有意差を認めた。歯周病の進行が骨密度低下の予測因子として有用であると考えられた。

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  • いつまでも健口生活 なぜわたしたちは歯を磨くのか

    森田 学

    さかえ: 月刊糖尿病ライフ   58 ( 12 )   24 - 25   2018.12

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  • 嚥下機能はオーラルディアドコキネシスと関連する

    竹内 倫子, 江國 大輔, 森田 学

    日本公衆衛生学会総会抄録集   77回   243 - 243   2018.10

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  • 口腔内ジメチルサルファイド濃度と全身疾患および服薬との関係

    横井 彩, 竹内 倫子, 宮井 久敬, 江國 大輔, 森田 学

    日本口臭学会会誌   9 ( 1 )   38 - 39   2018.5

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  • A大学病院障害者歯科において15年以上の疾患管理を行った患者のDMF歯数と受診状態の関連について

    森 貴幸, 山本 龍生, 森田 学

    口腔衛生学会雑誌   68 ( 増刊 )   168 - 168   2018.4

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  • 歯周組織における加齢に伴う酸化ストレスの増加に対するコーヒー摂取の影響

    小林 暉政, 東 哲司, 杉浦 嘉雄, 宮井 久敬, 米田 俊樹, 丸山 貴之, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   68 ( 増刊 )   182 - 182   2018.4

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  • 大学生における口腔関連QOLの変化に影響を及ぼす因子の検討 縦断研究

    佐保 輝, 竹内 真由, 稲生 智秋, Monirul Islam Md, 杉浦 嘉雄, 外山 直樹, 内田 瑶子, 横井 彩, 谷口 綾乃, 片岡 広太, 米田 俊樹, 東 哲司, 江國 大輔, 森田 学

    口腔衛生学会雑誌   68 ( 増刊 )   109 - 109   2018.4

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  • 歯科衛生士教育における模擬患者の違いによる実習効果の比較

    木村 優子, 江國 大輔, 森田 学, 太田 正美

    口腔衛生学会雑誌   68 ( 増刊 )   129 - 129   2018.4

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  • 大学生における歯ぎしりの自覚と不正咬合との関連 前向きコホート研究

    外山 直樹, 江國 大輔, 入江 浩一郎, 竹内 真由, 谷口 綾乃, 森田 学

    日本公衆衛生学会総会抄録集   76回   317 - 317   2017.10

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  • 勤務形態が口腔保健行動および口腔状態に与える影響

    外山 直樹, 入江 浩一郎, 谷口 綾乃, 江國 大輔, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   67 ( 3 )   206 - 206   2017.7

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  • 抗がん剤投与ラットへのカテキン局所塗布による抗酸化作用の検討

    小林 暉政, 宮井 久敬, 丸山 貴之, 友藤 孝明, 東 哲司, 米田 俊樹, 杉浦 嘉雄, 江國 大輔, 森田 学

    口腔衛生学会雑誌   67 ( 3 )   203 - 203   2017.7

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  • Expression of Salivary miR-203a-3p Was Related with Oral Health-Related Quality of Life in Healthy Volunteers. International journal

    Terumasa Kobayashi, Takaaki Tomofuji, Tatsuya Machida, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Akiko Hirose, Manabu Morita

    International journal of molecular sciences   18 ( 6 )   372 - 373   2017.6

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    Oral health-related quality of life (OHRQoL) is a multidimensional construct that involves subjective evaluation of an individual's oral health. Although it is difficult to evaluate OHRQoL biologically, recently, it has been reported that circulating microRNAs (miRNAs) in several body fluids could reflect various health conditions. The aim of this pilot study was to investigate whether salivary miRNAs expression differs according to OHRQoL in healthy volunteers. Forty-six volunteers (median age, 23.0 years) were recruited, and their OHRQoL was assessed using the Japanese version of the Oral Health Impact Profile (OHIP-J). Then, we compared salivary microRNA profiles of the high-OHRQoL group (&lt;= 25th percentile score of OHIP-J) and the low-OHRQoL group (&gt;= 75th percentile score of OHIP-J) using the polymerase chain reaction (PCR) array and the quantitative real-time PCR. There were no significant differences between the two groups in terms of oral health status. In the PCR array, miR-203a-3p and miR-30b-5p were significantly more expressed in the low-OHRQoL group (p &lt; 0.05). Quantitative real-time PCR assay also showed that miR-203a-3p was more highly expressed in the low-OHRQoL group than in the high-OHRQoL group (p &lt; 0.05). These observations suggest that expression of salivary miR-203a-3p was related with OHRQoL in healthy volunteers.

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  • Associations between dental knowledge, source of dental knowledge and oral health behavior in Japanese university students: A cross-sectional study. International journal

    Ayano Taniguchi-Tabata, Daisuke Ekuni, Shinsuke Mizutani, Mayu Yamane-Takeuchi, Kota Kataoka, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

    PloS one   12 ( 6 )   e0179298 - 12   2017.6

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    The aim of this study was to investigate the associations between dental knowledge, the source of dental knowledge and oral health behavior in a group of students at a university in Japan. A total of 2,220 university students (1,276 males, 944 females) volunteered to undergo an oral examination and answer a questionnaire. The questionnaire assessed dental knowledge, the source of dental knowledge and oral health behavior (e.g., daily frequency of tooth brushing, use of dental floss and regular dental checkups). The odds ratio and 95% confidence interval for oral health behavior based on dental knowledge and source of dental knowledge were calculated using logistic regression models. Of the participants, 1,266 (57.0%) students obtained dental knowledge from dental clinics, followed by school (39.2%) and television (29.1%). Logistic regression analyses indicated that use of dental floss was significantly associated with source of dental knowledge from dental clinics (P = 0.006). Receiving regular dental checkups was significantly associated with source of dental knowledge; the positive source was dental clinic (P &lt; 0.001) and the negative sources were school (P = 0.004) and television (P = 0.018). Dental clinic was the most common source of dental knowledge and associated with better oral health behavior among the Japanese university students in this study.

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  • 2型糖尿病患者における非外科的歯周治療による酸化ストレスの改善 ランダム化比較試験

    杉浦 嘉雄, 水野 裕文, 福原 大樹, 丸山 貴之, 江國 大輔, 米田 俊樹, 片岡 広太, 川端 勇也, 水谷 慎介, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   67 ( 増刊 )   119 - 119   2017.4

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  • 唾液中microRNAと歯周病の重症度との関連

    藤森 浩平, 米田 俊樹, 友藤 孝明, 江國 大輔, 東 哲司, 丸山 貴之, 町田 達哉, 水野 裕文, 森田 学

    口腔衛生学会雑誌   67 ( 増刊 )   168 - 168   2017.4

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  • Preventive Effects of Drinking Hydrogen-Rich Water on Gingival Oxidative Stress and Alveolar Bone Resorption in Rats Fed a High-Fat Diet. International journal

    Toshiki Yoneda, Takaaki Tomofuji, Muneyoshi Kunitomo, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Tatsuya Machida, Hisataka Miyai, Kouhei Fujimori, Manabu Morita

    Nutrients   9 ( 1 )   830 - 833   2017.1

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    Obesity induces gingival oxidative stress, which is involved in the progression of alveolar bone resorption. The antioxidant effect of hydrogen-rich water may attenuate gingival oxidative stress and prevent alveolar bone resorption in cases of obesity. We examined whether hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption in obese rats fed a high-fat diet. Male Fischer 344 rats (n = 18) were divided into three groups of six rats each: a control group (fed a regular diet and drinking distilled water) and two experimental groups (fed a high-fat diet and drinking distilled water or hydrogen-rich water). The level of 8-hydroxydeoxyguanosine was determined to evaluate oxidative stress. The bone mineral density of the alveolar bone was analyzed by micro-computerized tomography. Obese rats, induced by a high-fat diet, showed a higher gingival level of 8-hydroxydeoxyguanosine and a lower level of alveolar bone density compared to the control group. Drinking hydrogen-rich water suppressed body weight gain, lowered gingival level of 8-hydroxydeoxyguanosine, and reduced alveolar bone resorption in rats on a high-fat diet. The results indicate that hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption by limiting obesity.

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  • Validity of a New Kit Measuring Salivary Lactate Dehydrogenase Level for Screening Gingivitis. International journal

    Daisuke Ekuni, Mayu Yamane-Takeuchi, Kota Kataoka, Aya Yokoi, Ayano Taniguchi-Tabata, Hirofumi Mizuno, Hisataka Miyai, Yoko Uchida, Daiki Fukuhara, Yoshio Sugiura, Takaaki Tomofuji, Manabu Morita

    Disease markers   2017   9547956 - 9547956   2017

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    Aim. The aim of this study was to determine the usefulness of a new kit that can evaluate salivary lactate dehydrogenase (LD) level in real time for screening gingivitis. Materials and Methods. The study included 70 systemic healthy volunteers [29 males and 41 females; mean age +/- SD: 24.1 +/- 2.6 years]. Resting saliva was collected from each participant and LD level was evaluated in real time using the kit (a color-changing sheet with an integer scale ranging from 1 to 10). A dentist measured probing pocket depth, clinical attachment level, and the proportion of sites with bleeding on probing (% BOP) at six sites on all teeth. Gingivitis was diagnosed when the BOP value was &gt;= 20%. Results. Salivary LD level was positively correlated with mean % BOP (odds ratio: 1.47, 95% confidence interval: 1.132-1.916, and P &lt; 0.001) in a logistic regression model. The sensitivity and specificity of the kit were 0.89 and 0.98, respectively, at a cut-off value of 8.0 for LD level. Conclusions. The new kit for measurement of salivary LD level may be a useful tool to screen for gingivitis in young adults, which contributes to early detection of future periodontitis.

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  • The Serum Oxidative/Anti-oxidative Stress Balance Becomes Dysregulated in Patients with Non-alcoholic Steatohepatitis Associated with Hepatocellular Carcinoma

    Yasuyuki Shimomura, Akinobu Takaki, Nozomu Wada, Tetsuya Yasunaka, Fusao Ikeda, Takayuki Maruyama, Naofumi Tamaki, Daisuke Uchida, Hideki Onishi, Kenji Kuwaki, Shinichiro Nakamura, Kazuhiro Nouso, Yasuhiro Miyake, Kazuko Koike, Takaaki Tomofuji, Manabu Morita, Kazuhide Yamamoto, Hiroyuki Okada

    INTERNAL MEDICINE   56 ( 2 )   243 - 251   2017

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    Objective Oxidative stress is associated with the progression of chronic liver disease. Non-alcoholic fatty liver disease (NAFLD) is also an oxidative stress-related disease. However, the oxidative/anti-oxidative balance has not been fully characterized in NAFLD. The objective of the present study was to investigate the balance between oxidative stress and the anti-oxidative activity in NAFLD, including non-alcoholic steatohepatitis (NASH)-related hepatocellular carcinoma (HCC).
    Patients We recruited 69 patients with histologically proven NAFLD without HCC (NAFLD; n=58), and with NASH-related HCC (NASH-HCC; n=11). The 58 NAFLD patients included patients with non-alcoholic fatty liver (NAFL; n=14) and NASH (n=44).
    Methods The serum levels of reactive oxygen metabolites (ROM) and anti-oxidative markers (OXY) were determined and then used to calculate the oxidative index. The correlations among such factors as ROM, OXY, oxidative index, and clinical characteristics were investigated.
    Results In NAFLD, ROM positively correlated with the body mass index (BMI), hemoglobin A1c (HbA1c), C-reactive protein (CRP), and the histological grade or inflammatory scores, while only high HbA1c and CRP levels were significant factors that correlated with a higher ROM according to a multivariate analysis. OXY positively correlated with the platelet counts, albumin, and creatinine levels, while negatively correlating with age. However, it improved after treatment intervention. The oxidative index positively correlated with BMI, CRP, and HbA1c. The NASH-HCC patients exhibited a lower OXY than the NASH patients, probably due to the effects of aging.
    Conclusion Oxidative stress correlated with the levels of NASH activity markers, while the anti-oxidative function was preserved in younger patients as well as in patients with a well-preserved liver function. The NASH-HCC patients tended to be older and exhibited a diminished anti-oxidative function.

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  • 動機づけ面接法(MI)により高齢者の口腔衛生の改善ができた1症例.

    千神 八重子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   67 ( 1 )   29 - 34   2017

  • ラット歯周炎モデルに対する天然植物由来成分配合歯磨剤の効果.

    米田 俊樹, 東 哲司, 友藤 孝明, 江國 大輔, 片岡 広太, 山根 真由, 川端 勇也, 國友 宗義, 森田 学

    口腔衛生学会雑誌   67 ( 1 )   11 - 17   2017

  • Visualization of Oxidative Stress Induced by Experimental Periodontitis in Keap1-Dependent Oxidative Stress Detector-Luciferase Mice. International journal

    Kota Kataoka, Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Muneyoshi Kunitomo, Yoko Uchida, Daiki Fukuhara, Manabu Morita

    International journal of molecular sciences   17 ( 11 )   2016.11

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    The aim of this study was to investigate whether a Keap1-dependent oxidative stress detector-luciferase (OKD-LUC) mouse model would be useful for the visualization of oxidative stress induced by experimental periodontitis. A ligature was placed around the mandibular first molars for seven days to induce periodontitis. Luciferase activity was measured with an intraperitoneal injection of D-luciferin on days 0, 1, and 7. The luciferase activity in the periodontitis group was significantly greater than that in the control group at seven days. The expressions of heme oxygenase-1 (HO-1) and malondialdehyde in periodontal tissue were significantly higher in the periodontitis group than in the control group. Immunofluorescent analysis confirmed that the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) occurred more frequently in the periodontitis group than in the control group. This study found that under oxidative stress induced by experimental periodontitis, the Nrf2/antioxidant defense pathway was activated and could be visualized from the luciferase activity in the OKD-LUC model. Thus, the OKD-LUC mouse model may be useful for exploring the mechanism underlying the relationship between the Nrf2/antioxidant defense pathway and periodontitis by enabling the visualization of oxidative stress over time.

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  • School-Based Fluoride Mouth-Rinse Program Dissemination Associated With Decreasing Dental Caries Inequalities Between Japanese Prefectures: An Ecological Study.

    Yusuke Matsuyama, Jun Aida, Katsuhiko Taura, Kazunari Kimoto, Yuichi Ando, Hitoshi Aoyama, Manabu Morita, Kanade Ito, Shihoko Koyama, Akihiro Hase, Toru Tsuboya, Ken Osaka

    Journal of epidemiology   26 ( 11 )   563 - 571   2016.11

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    © 2016 Yusuke Matsuyama et al.Background: Dental caries inequalities still severely burden individuals&#039; and society&#039;s health, even in countries where fluoride toothpastes are widely used and the incidence of dental caries has been decreasing. School-based fluoride mouth-rinse (S-FMR) programs, a population strategy for dental caries prevention, might decrease dental caries inequalities. This study investigated the association between S-FMR and decreasing dental caries prevalence and caries-related inequalities in 12-year-olds by Japanese prefecture. Methods: We conducted an ecological study using multi-year prefecture-level aggregated data of children born between 1994 and 2000 in all 47 Japanese prefectures. Using two-level linear regression analyses (birth year nested within prefecture), the association between S-FMR utilization in each prefecture and 12-year-olds&#039; decayed, missing, or filled permanent teeth (DMFT), which indicates dental caries experience in their permanent teeth, were examined. Variables that could explain DMFT inequalities between prefectures, such as dental caries experience at age 3 years, dentist density, and prefectural socioeconomic circumstances, were also considered. Results: High S-FMR utilization was significantly associated with low DMFT at age 12 (coefficient-0.011; 95% confidence interval,-0.018 to-0.005). S-FMR utilization explained 25.2% of the DMFT variance between prefectures after considering other variables. Interaction between S-FMR and dental caries experience at age 3 years showed that S-FMR was significantly more effective in prefectures where the 3-year-olds had high levels of dental caries experience. Conclusions: S-FMR, administered to children of all socioeconomic statuses, was associated with lower DMFT. Utilization of S-FMR reduced dental caries inequalities via proportionate universalism.

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  • miR‑1246 and miR‑4644 in salivary exosome as potential biomarkers for pancreatobiliary tract cancer. International journal

    Tatsuya Machida, Takaaki Tomofuji, Takayuki Maruyama, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Hisataka Miyai, Hirofumi Mizuno, Hironari Kato, Koichiro Tsutsumi, Daisuke Uchida, Akinobu Takaki, Hiroyuki Okada, Manabu Morita

    Oncology reports   36 ( 4 )   2375 - 81   2016.10

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    Pancreatobiliary tract cancer is a highly fatal cancer. Detection of pancreatobiliary tract cancer is difficult because it lacks typical clinical symptoms and because of its anatomical location. Biomarker discovery is therefore important to detect pancreatobiliary tract cancer in its early stage. A study demonstrated that expression levels of miR-1246, miR-3976, miR-4306, and miR-4644 in serum exosomes were higher in pancreatic cancer patients than these levels in healthy control participants. Supposing that microRNA (miRNA) expression profiles in saliva are similar to those in serum, four miRNAs (miR-1246, miR-3976, miR-4306, and miR-4644) in salivary exosomes may also be useful for detection of pancreatobiliary tract cancer. In this study, it was examined whether these miRNAs could be used as biomarkers for pancreatobiliary tract cancer. Twelve pancreatobiliary tract cancer patients and 13 healthy control participants were analyzed as a cancer and a control group, respectively. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. Using quantitative real-time PCR (RT-qPCR), the relative expression ratios of miR-1246 and miR-4644 were significantly higher in the cancer group than these ratios in the control group. Receiver operating characteristic (ROC) curves were constructed to analyze the discrimination power of these miRNAs. For miR-1246, the results yielded an area under the curve (AUC) of 0.814 (P=0.008). For miR-4644, the results yielded an AUC of 0.763 (P=0.026). For the combination of miR-1246 and miR-4644, the results yielded an increased AUC of 0.833 (P=0.005). This pilot study suggests that miR-1246 and miR-4644 in salivary exosomes could be candidate biomarkers for pancreatobiliary tract cancer.

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  • Oxidative stress balance is dysregulated and represents an additional target for treating cholangiocarcinoma. International journal

    Daisuke Uchida, Akinobu Takaki, Hisashi Ishikawa, Yasuko Tomono, Hironari Kato, Koichiro Tsutsumi, Naofumi Tamaki, Takayuki Maruyama, Takaaki Tomofuji, Ryuichiro Tsuzaki, Tetsuya Yasunaka, Kazuko Koike, Hiroshi Matsushita, Fusao Ikeda, Yasuhiro Miyake, Hidenori Shiraha, Kazuhiro Nouso, Ryuichi Yoshida, Yuzo Umeda, Susumu Shinoura, Takahito Yagi, Toshiyoshi Fujiwara, Manabu Morita, Masaki Fukushima, Kazuhide Yamamoto, Hiroyuki Okada

    Free radical research   50 ( 7 )   732 - 43   2016.7

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    Background: Pancreatico-biliary malignancies exhibit similar characteristics, including obesity-related features and poor prognosis, and require new treatment strategies. Oxidative stress is known to induce DNA damage and carcinogenesis, and its reduction is viewed as being favorable. However, it also has anti-infection and anti-cancer functions that need to be maintained. To reveal the effect of oxidative stress on cancer progression, we evaluated oxidative stress and anti-oxidative balance in pancreatic cancer (PC) and cholangiocarcinoma (CC) patients, as well as the effect of add-on antioxidant treatment to chemotherapy in a mouse cholangiocarcinoma model.
    Methods: We recruited 84 CC and 80 PC patients who were admitted to our hospital. Serum levels of reactive oxygen metabolites (ROM) and the anti-oxidative OXY-adsorbent test were determined and the balance of these tests was defined as an oxidative index. A diabetic mouse-based cholangiocarcinoma model was utilized to evaluate the effects of add-on antioxidant therapy on cholangiocarcinoma chemotherapy.
    Results: Serum ROM was higher and anti-oxidant OXY was lower in CC patients with poor outcomes. These parameters were not significantly different in PC patients. In mice, vitamin E administration induced antioxidant hemeoxygenase (HO)-1 protein expression in cancer tissue, while the number of stem-like cells increased. L-carnitine administration improved intestinal microbiome and biliary acid balance, upregulated the hepatic mitochondrial membrane uptake related gene Cpt1 in non-cancerous tissue, and did not alter stem-like cell numbers.
    Conclusion: Oxidative stress balance was dysregulated in cholangiocarcinoma with poor outcome. The mitochondrial function-supporting agent L-carnitine is a good candidate to control oxidative stress conditions.

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  • MicroRNAs as serum biomarkers for periodontitis. International journal

    Takaaki Tomofuji, Toshiki Yoneda, Tatsuya Machida, Daisuke Ekuni, Tetsuji Azuma, Kota Kataoka, Takayuki Maruyama, Manabu Morita

    Journal of clinical periodontology   43 ( 5 )   418 - 25   2016.5

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    Aim: Studies demonstrated that periodontitis modulates microRNA (miRNAs) expression rates in periodontal tissue. However, the relationship between periodontitis and miRNAs profile in circulation remains unclear. In this study, we investigated the effects of periodontitis on serum miRNAs profile in a rat model.
    Material and Methods: Male Wistar rats (n=32, 8weeks old) were divided into four groups of eight rats each. The control groups received no treatment for 2 or 4weeks. In the other two groups, periodontitis was ligature induced for 2 or 4weeks. Serum miRNAs expression profiles of each group were compared.
    Results: Ligation around teeth induced periodontal inflammation at 2weeks and periodontal tissue destruction at 4weeks. Microarray results showed that 25 miRNAs were expressed with a &lt;0.5 or &gt;2 difference between the control and periodontitis groups at 4weeks. Results of real-time PCR revealed that the periodontitis group up-regulated expression rates of serum miR-207 and miR-495 at 2weeks, and miR-376b-3p at 4weeks (p&lt;0.05).
    Conclusion: Serum miRNAs (miR-207, miR-495, and miR-376b-3p) could be valuable biomarkers for periodontitis.

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  • 食道癌の術後患者に対する歯科専門職による口腔のケアの効果

    水野 裕文, 水谷 慎介, 丸山 貴之, 横井 彩, 谷口 彩乃, 山中 玲子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   66 ( 2 )   219 - 219   2016.4

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  • 歯肉炎のスクリーニングにおける唾液中LDH簡易測定キットの有効性

    吉田 宗生, 加藤 壮真, 山根 真由, 江國 大輔, 横井 彩, 丸山 貴之, 山中 玲子, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   66 ( 2 )   294 - 294   2016.4

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  • 口腔内アセトアルデヒド濃度と舌背の細菌数との関係

    加藤 壮真, 吉田 宗生, 横井 彩, 丸山 貴之, 江國 大輔, 山根 真由, 山中 玲子, 友藤 孝明, 森田 学

    口腔衛生学会雑誌   66 ( 2 )   296 - 296   2016.4

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  • 口腔ケアの感染症予防・治療への有効性 上部消化癌患者と健常者における舌苔と口腔内アセトアルデヒド濃度との関連

    山中 玲子, 横井 彩, 丸山 貴之, 江國 大輔, 森田 学

    感染症学雑誌   90 ( 2 )   168 - 169   2016.3

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  • 口腔ケアの感染症予防・治療への有効性 上部消化癌患者と健常者における舌苔と口腔内アセトアルデヒド濃度との関連

    山中 玲子, 横井 彩, 丸山 貴之, 江國 大輔, 森田 学

    日本化学療法学会雑誌   64 ( 2 )   314 - 315   2016.3

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  • Self-efficacy and progression of periodontal disease: a prospective cohort study. International journal

    Shinsuke Mizutani, Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

    Journal of clinical periodontology   42 ( 12 )   1083 - 9   2015.12

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    Aim: The purpose of this longitudinal study was to investigate whether self/efficacy scale for self-care (SESS) was related to changes in oral health behaviours and progression of periodontal disease in Japanese university students.
    Material and Methods: Students (n = 533) who were interested in receiving oral health examinations before entering the university and before graduation were included in the analysis. Self-efficacy was assessed using SESS, which uses three subscales: self-efficacy for brushing of the teeth (SE-BR), for dentist consultations (SE-DC) and for dietary habits. The informations about oral health behaviours were also collected. For all participants, oral hygiene instructions was performed at baseline. After 3 years, the oral health behaviours and the periodontal condition were re-assessed.
    Results: The progression of periodontal disease was related to low baseline SE-BR. Logistic regression analysis showed that progression of periodontal disease risk during a 3-year period was associated with low baseline SE-BR (OR: 1.516; 95% CI: 1.010-2.275; p &lt; 0.05). The increase in the score of SE-DC was related to the increase in regular dental checkups (p &lt; 0.05).
    Conclusion: Low SESS was associated with progression of periodontal disease and improvement of oral health behaviours in university students who received oral hygiene instructions at baseline.

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  • A大学病院障害者歯科診療室における抜歯の傾向について

    森 貴幸, 山本 龍生, 森田 学

    日本公衆衛生学会総会抄録集   74回   441 - 441   2015.10

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  • Gingival condition and tooth-brushing behavior after alcohol consumption

    S. Mizutani, D. Ekuni, T. Tomofuji, M. Yamane, T. Azuma, Y. Iwasaki, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   50 ( 4 )   494 - 499   2015.8

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    Background and ObjectiveVarious studies have reported the relationship between alcohol consumption and gingival condition. However, they focus on the direct effects of alcohol consumption or alcohol sensitivity on gingival condition, and it is unclear how oral health behaviors relate these relationships. The aims of this study were to assess the inter-relationships between gingival condition, tooth-brushing behavior after drinking alcohol and alcohol sensitivity in university students who drink more than once per week on average.
    Material and MethodsA total of 808 students (541 males, 267 females) that habitually consume alcohol were analyzed. The disease activity of gingival condition was assessed as the percentage of bleeding on probing (%BOP). Additional information regarding alcohol sensitivity and oral health behaviors, including tooth-brushing behavior after drinking, were also collected.
    ResultsThirteen percent of the current participants reported neglecting tooth-brushing after drinking, and their alcohol consumption was higher than those who did not neglect tooth-brushing. Logistic regression analysis showed that high %BOP (%BOP 20) was associated with male (OR=1.53; 95% CI, 1.01-2.33), neglect of tooth-brushing after drinking (OR=2.60; 95% CI, 1.20-5.61) and debris index (OR=8.38; 95% CI, 4.24-16.60) in participants with low alcohol sensitivity. In participants with high alcohol sensitivity, high %BOP was associated with debris index (OR=7.60; 95% CI, 3.12-18.51), but not with any oral health behaviors.
    ConclusionsThe study revealed that alcohol consumption was indirectly related to gingival disease activity through the neglect of tooth-brushing after drinking alcohol in university students with low alcohol sensitivity.

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  • Drinking Hydrogen-Rich Water Has Additive Effects on Non-Surgical Periodontal Treatment of Improving Periodontitis: A Pilot Study. International journal

    Tetsuji Azuma, Mayu Yamane, Daisuke Ekuni, Yuya Kawabata, Kota Kataoka, Kenta Kasuyama, Takayuki Maruyama, Takaaki Tomofuji, Manabu Morita

    Antioxidants (Basel, Switzerland)   4 ( 3 )   513 - 22   2015.7

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    Oxidative stress is involved in the pathogenesis of periodontitis. A reduction of oxidative stress by drinking hydrogen-rich water (HW) might be beneficial to periodontal health. In this pilot study, we compared the effects of non-surgical periodontal treatment with or without drinking HW on periodontitis. Thirteen patients (3 women, 10 men) with periodontitis were divided into two groups: The control group (n = 6) or the HW group (n = 7). In the HW group, participants consumed HW 4-5 times/day for eight weeks. At two to four weeks, all participants received non-surgical periodontal treatment. Oral examinations were performed at baseline, two, four and eight weeks, and serum was obtained at these time points to evaluate oxidative stress. At baseline, there were no significant differences in periodontal status between the control and HW groups. The HW group showed greater improvements in probing pocket depth and clinical attachment level than the control group at two, four and eight weeks (p < 0.05). The HW group also exhibited an increased serum level of total antioxidant capacity at four weeks, compared to baseline (p < 0.05). Drinking HW enhanced the effects of non-surgical periodontal treatment, thus improving periodontitis.

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  • Impact of Oral Commensal Bacteria on Degradation of Periodontal Connective Tissue in Mice. International journal

    Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Manabu Morita, Yoshihiro Shimazaki, Richard P Darveau

    Journal of periodontology   86 ( 7 )   899 - 905   2015.7

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    Background: Innate and adaptive immunosurveillance mechanisms in response to the normal commensal bacteria can affect periodontal innate defense status. However, it is still unclear how commensal bacteria contribute to the inflammatory responses of junctional epithelium (JE) and periodontal connective tissue (PCT). The aim of the present study is to investigate the contribution of commensal bacteria on inflammatory responses in JE and PCT in mice.
    Methods: The periodontal tissue of germ-free (GF) and specific-pathogen-free (SPF) mice were compared at age 11 to 12 weeks (n = 6 per group). In this study, the number of neutrophils and expression of intercellular adhesion molecule (ICAM)-1, fibroblast growth factor receptor (FGFR)-1, matrix metalloproteinase (MMP)-1, and MMP-8 within the JE and the PCT are evaluated. The collagen density was also determined in PCT stained with picrosirius red (PSR). PSR staining combined with or without polarized light microscopy has been used to assess the organization and maturation of collagen matrix.
    Results: In the present findings, the area of JE in SPF mice was significantly greater than that in GF mice (P &lt;0.05). In addition, the JE and PCT in SPF mice showed greater migration of neutrophils and higher expression of ICAM-1, FGFR-1, MMP-1, and MMP-8 than those in GF mice (P &lt;0.05). Furthermore, the density of collagen in PCT in SPF mice was lower compared to GF mice (P &lt;0.05).
    Conclusion: These results indicate that commensal bacteria induced a low-grade inflammatory state in JE and that such conditions may contribute to degradation of collagen in PCT in mice.

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  • 日本歯周病学会会員の喫煙に関する質問票調査

    稲垣 幸司, 王 宝禮, 埴岡 隆, 藤井 健男, 両角 俊哉, 伊藤 弘, 山本 龍生, 森田 学

    日本歯周病学会会誌   57 ( 2 )   100 - 106   2015.7

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    日本歯周病学会会員の喫煙状況を把握するために層化無作為抽出による質問票調査を行った。対象は、631名(男性62.4%、回収率:42.1%)で、30-39歳が最も多く30.3%を占めた。職種の内訳は、歯科医師75.4%、歯科衛生士22.5%、歯科技工士0.2%、学生0.3%、その他1.6%であった。所属別では、大学・大学院・短大15.2%、附属病院・関連病院8.1%、開業歯科医院71.6%、その他5.1%で、学会資格でみると、指導医4.9%、専門医13.0%、認定医8.6%、認定歯科衛生士13.2%、一般会員60.4%であった。勤務先の脱タバコ対策は、敷地内禁煙48.2%、建物内禁煙34.9%、一方、家族・同居者に喫煙者がいるものが30.7%となった。喫煙歴は、非喫煙者376名(59.6%)、前喫煙者211名(33.4%)、喫煙者44名(7.0%)で、職種別喫煙率は、歯科医師8.4%、歯科衛生士2.1%で、歯科医師が有意に高かった(P&lt;0.05)。資格の有無別喫煙率は、指導医、専門医、認定医7.8%、一般会員8.7%となった。喫煙者の年齢層別喫煙率は、20-29歳3.1%、30-39歳8.9%、40-49歳6.9%、50-59歳6.2%、60-69歳8.0%で、30-39歳が最も高かった。なお、喫煙者の70.5%が禁煙を試みていたが、81.8%が関心期であった。本調査結果が、日本歯周病学会会員の歯周病治療における禁煙支援を積極的に推進していくことに活かされていくことが望まれる。(著者抄録)

    DOI: 10.2329/perio.57.100

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  • 岡山大学病院周術期管理センターにおける術前プラークフリーの実際

    丸山 貴之, 水谷 慎介, 山中 玲子, 横井 彩, 町田 達哉, 米田 俊樹, 片岡 広太, 川端 勇也, 森田 学

    口腔衛生学会雑誌   65 ( 3 )   312 - 312   2015.4

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  • 多職種連携の中で専門的口腔衛生管理を行い、経口摂取を継続しながら化学放射線治療が完遂できた一症例

    志茂 加代子, 丸山 貴之, 横井 彩, 山中 玲子, 三浦 留美, 江國 大輔, 森田 学

    口腔衛生学会雑誌   65 ( 2 )   187 - 187   2015.4

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  • Relationship between xerostomia and gingival condition in young adults

    S. Mizutani, D. Ekuni, T. Tomofuji, T. Azuma, K. Kataoka, M. Yamane, Y. Iwasaki, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   50 ( 1 )   74 - 79   2015.2

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    Background and ObjectiveXerostomia is a subjective symptom of dryness in the mouth. Although a correlation between xerostomia and oral conditions in the elderly has been reported, there are few such studies in the young adults. The aim of this study was to examine the relationship of xerostomia with the gingival condition in university students.
    Material and MethodsA total of 2077 students (1202 male subjects and 875 female subjects), 18-24years of age, were examined. The disease activity and severity of the gingival condition were assessed as the percentage of teeth with bleeding on probing (%BOP) and the presence of teeth with probing pocket depth of 4mm, respectively. Additional information on xerostomia, oral health behaviors, coffee/tea intake and nasal congestion was collected via a questionnaire. Path analysis was used to test pathways from xerostomia to the gingival condition.
    ResultsOne-hundred and eighty-three (8.8%) students responded that their mouths frequently or always felt dry. Xerostomia was related to %BOP and dental plaque formation, but was not related to the presence of probing pocket depth 4mm. In the structural model, xerostomia was related to dental plaque formation (p&lt;0.01), and a lower level of dental plaque formation was associated with a lower %BOP. Xerostomia was associated with coffee/tea intake (p&lt;0.01) and nasal congestion (p&lt;0.001).
    ConclusionXerostomia was indirectly related to gingival disease activity through the accumulation of dental plaque. Nasal congestion and coffee/tea intake also affected xerostomia. These findings suggest that xerostomia should be considered in screening for gingivitis risk in young adults.

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  • Relationship between acetaldehyde concentration in mouth air and tongue coating volume

    Aya Yokoi, Takayuki Maruyama, Reiko Yamanaka, Daisuke Ekuni, Takaaki Tomofuji, Haruhiko Kashiwazaki, Yutaka Yamazaki, Manabu Morita

    JOURNAL OF APPLIED ORAL SCIENCE   23 ( 1 )   64 - 70   2015.1

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    Objective: Acetaldehyde is the first metabolite of ethanol and is produced in the epithelium by mucosal ALDH, while higher levels are derived from microbial oxidation of ethanol by oral microflora such as Candida species. However, it is uncertain whether acetaldehyde concentration in human breath is related to oral condition or local production of acetaldehyde by oral microflora. The aim of this pilot study was to investigate the relationship between physiological acetaldehyde concentration and oral condition in healthy volunteers. Material and Methods: Sixty-five volunteers (51 males and 14 females, aged from 20 to 87 years old) participated in the present study. Acetaldehyde concentration in mouth air was measured using a portable monitor. Oral examination, detection of oral Candida species and assessment of alcohol sensitivity were performed. Results: Acetaldehyde concentration [median (25%, 75%)] in mouth air was 170.7 (73.5, 306.3) ppb. Acetaldehyde concentration in participants with a tongue coating status score of 3 was significantly higher than in those with a score of 1 (p&lt;0.017). After removing tongue coating, acetaldehyde concentration decreased significantly (p&lt;0.05). Acetaldehyde concentration was not correlated with other clinical parameters, presence of Candida species, smoking status or alcohol sensitivity. Conclusion: Physiological acetaldehyde concentration in mouth air was associated with tongue coating volume.

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  • 歯周疾患と糖尿病等との関係に着目した歯科保健指導方法の開発等に関する研究

    森田学, 和田淳, 友藤孝明, 江國大輔, 安藤雄一, 荻野景規

    厚生労働科学研究費補助金 循環器疾患・糖尿病等生活習慣病対策総合研事業 平成26年度 総括・分担研究報告書   2015

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  • Serum Oxidative/anti-oxidative Stress Balance Is Dysregulated in Potentially Pulmonary Hypertensive Patients with Liver Cirrhosis: A Case Control Study.

    Masako Terao, Akinobu Takaki, Takayuki Maruyama, Hiroki Oe, Tetsuya Yasunaka, Naofumi Tamaki, Kazufumi Nakamura, Takaaki Tomofuji, Takahito Yagi, Hiroshi Sadamori, Yuzo Umeda, Susumu Shinoura, Ryuichi Yoshida, Kazuhiro Nouso, Daisuke Ekuni, Kazuko Koike, Fusao Ikeda, Hidenori Shiraha, Manabu Morita, Hiroshi Ito, Toshiyoshi Fujiwara, Kazuhide Yamamoto

    Internal medicine (Tokyo, Japan)   54 ( 22 )   2815 - 26   2015

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    OBJECTIVE: Hepatopulmonary syndrome (HPS) is characterized by vascular dilatation and hyperdynamic circulation, while portopulmonary hypertension (POPH) is characterized by vasoconstriction with fibrous obliteration of the vascular bed. Vasoactive molecules such as nitric oxide (NO) are candidate factors for cirrhotic complications associated with these diseases. However, oxidative stress balance is not well characterized in HPS and POPH. The present objective is to investigate the oxidative stress and anti-oxidative stress balance and NO pathway balance in patients with potential HPS and POPH. METHODS: We recruited patients with decompensated cirrhosis (n=69) admitted to our hospital as liver transplantation candidates. Patients exhibiting partial pressure of oxygen lower than 80 mmHg and alveolar-arterial oxygen gradient (AaDO2) ≥15 mmHg were categorized as potentially having HPS (23 of 69 patients). Patients exhibiting a tricuspid regurgitation pressure gradient ≥25 mmHg were categorized as potentially having POPH (29 of 61 patients). Serum reactive oxygen metabolites were measured and anti-oxidative OXY-adsorbent test (OXY) were performed, and the balance of these tests was defined as the oxidative index. The correlation between these values and the clinical characteristics of the patients were assessed in a cross-sectional study. RESULTS: Potential HPS patients exhibited no correlation with oxidative stress markers. Potential POPH patients exhibited lower OXY (p=0.037) and higher oxidative index values (p=0.001). Additionally, the vascular NO synthase enzyme inhibiting protein, asymmetric dimethylarginine, was higher in potential POPH patients (p=0.049). The potential POPH patients exhibited elevated AaDO2, suggesting the presence of pulmonary shunting. CONCLUSION: Potential POPH patients exhibited elevated oxidative stress with decreased anti-oxidative function accompanied by inhibited NO production. Anti-oxidants represent a candidate treatment for potential POPH patients.

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  • 歯周病メインテナンス期患者に対する血液検査による生活習慣病スクリーニングの検討

    町田達哉, 友藤孝明, 江國大輔, 東 哲司, 竹内倫子, 丸山貴之, 川端勇也, 森田 学

    口腔衛生会誌   2015

  • 大学生における早食いと肥満の関係

    山根真由, 江國大輔, 森田 学

    厚生の指標   2015

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  • 塩化セチルピリジニウム配合トローチ剤の口臭抑制効果

    友藤 孝明, 片岡 広太, 川端 勇也, 水谷 慎介, 江國 大輔, 森田 学

    日口臭誌   2015

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  • 口腔内アセトアルデヒド濃度と舌苔スコアとの関係

    横井 彩, 江國大輔, 森田 学

    日本歯科評論   2015

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  • Application of coenzyme Q10 for accelerating soft tissue wound healing after tooth extraction in rats. International journal

    Toshiki Yoneda, Takaaki Tomofuji, Yuya Kawabata, Daisuke Ekuni, Tetsuji Azuma, Kota Kataoka, Muneyoshi Kunitomo, Manabu Morita

    Nutrients   6 ( 12 )   5756 - 69   2014.12

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    Accelerating wound healing after tooth extraction is beneficial in dental treatment. Application of antioxidants, such as reduced coenzyme Q10 (rCoQ10), may promote wound healing after tooth extraction. In this study, we examined the effects of topical application of rCoQ10 on wound healing after tooth extraction in rats. After maxillary first molars were extracted, male Fischer 344 rats (8 weeks old) (n = 27) received topical application of ointment containing 5% rCoQ10 (experimental group) or control ointment (control group) to the sockets for 3 or 8 days (n = 6-7/group). At 3 days after extraction, the experimental group showed higher collagen density and lower numbers of polymorphonuclear leukocytes in the upper part of socket, as compared to the control group (p &lt; 0.05). Gene expression of interleukin-1, tumor necrosis factor-alpha and nuclear factor-kappa B were also lower in the experimental group than in the control group (p &lt; 0.05). At 8 days after tooth extraction, there were no significant differences in collagen density, number of polymorphonuclear leukocytes and bone fill between the groups. Our results suggest that topical application of rCoQ10 promotes wound healing in the soft tissue of the alveolar socket, but that rCoQ10 has a limited effect on bone remodeling in rats.

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  • Management of lacerated and swollen tongue after convulsive seizure with a mouth protector: interprofessional collaboration including dentists in intensive care.

    Reiko Yamanaka, Yoshihiko Soga, Yoshie Moriya, Akemi Okui, Tetsuo Takeuchi, Kenji Sato, Hiroshi Morimatsu, Manabu Morita

    Acta medica Okayama   68 ( 6 )   375 - 8   2014.12

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    We encountered a 74-year-old male patient with tongue laceration after convulsive seizures under intensive care. The tongue showed severe swelling, and the right ventral surface had been lacerated by his isolated and pointed right lower canine. Our university hospital has established a perioperative management center, and is promoting interprofessional collaboration, including dentists, in perioperative management. Dentists collaborating in the perioperative management center took dental impressions, with the support of anesthesiologists who opened the patient's jaw under propofol sedation, to produce a mouth protector. By raising the patient's bite, the completed mouth protector prevented the isolated tooth from contacting the tongue and protected the lacerated wound. Use of the mouth protector prevented the lacerated tongue from coming into contact with the pointed tooth, and the tongue healed gradually. These findings underscore that interprofessional collaboration including dentists can improve the quality of medical care.

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  • Severe periodontitis is inversely associated with coffee consumption in the maintenance phase of periodontal treatment. International journal

    Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Noriko Takeuchi, Takayuki Maruyama, Shinsuke Mizutani, Kota Kataoka, Yuya Kawabata, Manabu Morita

    Nutrients   6 ( 10 )   4476 - 90   2014.10

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    This cross-sectional study addressed the relationship between coffee consumption and periodontitis in patients during the maintenance phase of periodontal treatment. A total of 414 periodontitis patients in the maintenance phase of periodontal treatment completed a questionnaire including items related to coffee intake and underwent periodontal examination. Logistic regression analysis showed that presence of moderate/severe periodontitis was correlated with presence of hypertension (Odds Ratio (OR) = 1.99, p &lt; 0.05), smoking (former, OR = 5.63, p &lt; 0.01; current, OR = 6.81, p = 0.076), number of teeth present (OR = 0.89, p &lt; 0.001), plaque control record &gt;= 20% (OR = 1.88, p &lt; 0.05), and duration of maintenance phase (OR = 1.07, p &lt; 0.01). On the other hand, presence of severe periodontitis was correlated with smoking (former, OR = 1.35, p = 0.501; current, OR = 3.98, p &lt; 0.05), coffee consumption (&gt;= 1 cup/day, OR = 0.55, p &lt; 0.05), number of teeth present (OR = 0.95, p &lt; 0.05), and bleeding on probing &gt;= 20% (OR = 3.67, p &lt; 0.001). There appears to be an inverse association between coffee consumption (&gt;= 1 cup/day) and prevalence of severe periodontitis in the maintenance phase of periodontal treatment.

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  • Relationships between eating quickly and weight gain in Japanese university students: a longitudinal study. International journal

    Mayu Yamane, Daisuke Ekuni, Shinsuke Mizutani, Kota Kataoka, Masami Sakumoto-Kataoka, Yuya Kawabata, Chie Omori, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

    Obesity (Silver Spring, Md.)   22 ( 10 )   2262 - 6   2014.10

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    ObjectiveMany cross-sectional studies have reported a relationship between overweight/obesity and eating quickly, but there have been few longitudinal studies to address this relationship in younger populations. The purpose of this prospective longitudinal study was to investigate whether eating quickly was related to being overweight in Japanese university students.
    MethodsOf 1,396 students who underwent a general examination and completed questionnaires at the start of university and before graduation, 1,314 students (676 male and 638 female) of normal body composition [body mass index (BMI)&lt;25 kg m(-2)] at baseline were included in the analysis. The questionnaires included speed of eating and other lifestyle factors. After a 3-year follow-up, the students whose BMIs were25 kg m(-2) were defined as overweight.
    ResultsIn this study, 38 participants (2.9%) became overweight. In the logistic regression analysis, the risk of being overweight was increased in males [adjusted odds ratio (OR): 2.77; 95% confidence interval (CI): 1.33-5.79; P&lt;0.01] and in those who ate quickly at baseline (OR: 4.40; 95% CI: 2.22-8.75; P&lt;0.001).
    ConclusionsEating quickly may predict risk of being overweight in Japanese university students.

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  • 近年における永久歯の萠出年齢の推移

    竹内 倫子, 友藤 孝明, 江國 大輔, 山中 玲子, 古田 美智子, 山本 龍生, 恒石 美登里, 森田 学

    日本公衆衛生学会総会抄録集   73回   419 - 419   2014.10

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  • Occlusal disharmony accelerates the initiation of atherosclerosis in apoE knockout rats. International journal

    Daisuke Ekuni, Toshiki Yoneda, Yasumasa Endo, Kenta Kasuyama, Koichiro Irie, Shinsuke Mizutani, Tetsuji Azuma, Takaaki Tomofuji, Manabu Morita

    Lipids in health and disease   13   144 - 144   2014.9

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    Background: Psychosocial stress is one of the risk factors for atherosclerosis. As occlusal disharmony induces psychological stress, we hypothesized that psychological stress by occlusal disharmony accelerates atherosclerosis. The aim of this study was to investigate the effects of occlusal disharmony on the initiation of atherosclerosis in apolipoprotein E (apoE) knockout rats.
    Methods: Fourteen male apoE-knockout rats (age; 8 weeks) (Sprague-Dawley strain background) were divided into two groups of seven rats: the occlusal disharmony group and the no treatment (control) group. In the occlusal disharmony group, the maxillary molar cusps were cut off for the 8-week experimental period.
    Results: In the occlusal disharmony group, the percentages of the area of total aortic lumen occupied by plaques and lipid were significantly higher than those in the control group (p &lt; 0.05, t-test). The occlusal disharmony group also showed significantly higher serum levels of very low-density lipoprotein-cholesterol (VLDL) and low-density lipoprotein-cholesterol (LDL), plasma levels of corticosterone (1.9, 1.3 and 1.3 times, respectively), higher aortic protein expression levels of vascular cell adhesion molecule-1 (VCAM1) and intercellular adhesion molecule-1 (ICAM1) (1.5 and 1.4 times, respectively), and higher aortic gene expression of levels of VCAM1 and Toll-like receptor 4 (TLR4) (1.9 and 4.3 times, respectively), as compared to the control group (p &lt; 0.05). However, there were no significant differences in serum levels of oxidized LDL, reactive oxygen metabolites and C-reactive protein between the two groups.
    Conclusion: In apoE knockout rats, occlusal disharmony may induce VCAM1, ICAM1 and TLR4 expression and accelerate the initiation of atherosclerosis.

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  • Relationship between increases in BMI and changes in periodontal status: a prospective cohort study. International journal

    Daisuke Ekuni, Shinsuke Mizutani, Azusa Kojima, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Toshiki Yoneda, Michiko Furuta, Nobuoki Eshima, Yoshiaki Iwasaki, Manabu Morita

    Journal of clinical periodontology   41 ( 8 )   772 - 8   2014.8

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    Aim: The purpose of this prospective cohort study was to investigate whether body mass index (BMI) and oral health behaviour are related to changes in periodontal status in Japanese university students.
    Materials and Methods: Students (n = 224) who were interested in receiving oral health examinations before entering university and before graduation were included in the analysis. Subjects were investigated regarding the correlations of oral health behaviours and increases in BMI with the percentage of bleeding on probing (% BOP) and Community Periodontal Index (CPI) scores as indicators of changes in periodontal status.
    Results: The risk of increased % BOP was associated with the non-use of dental floss (adjusted odds ratio [OR]: 3.11; 95% confidence interval [CI]: 1.31-7.37; p &lt; 0.05), whereas the risk of increased CPI score was associated with increases in BMI (OR: 1.95; 95% CI: 1.05-3.65; p &lt; 0.05) and simplified oral hygiene index score (OR: 2.28; 95% CI: 1.23-4.22; p &lt; 0.01).
    Conclusion: Increases in BMI were associated with worsening of periodontal status, defined as increased CPI score in Japanese university students, whereas lack of inter-dental cleaning was associated with exacerbated gingival bleeding.

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  • Effects of hydrogen-rich water on aging periodontal tissues in rats. International journal

    Takaaki Tomofuji, Yuya Kawabata, Kenta Kasuyama, Yasumasa Endo, Toshiki Yoneda, Mayu Yamane, Tetsuji Azuma, Daisuke Ekuni, Manabu Morita

    Scientific reports   4   5534 - 5534   2014.7

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    Oxidative damage is involved in age-related inflammatory reactions. The anti-oxidative effects of hydrogen-rich water suppress oxidative damage, which may aid in inhibiting age-related inflammatory reactions. We investigated the effects of drinking hydrogen-rich water on aging periodontal tissues in healthy rats. Four-month-old male Fischer 344 rats (n = 12) were divided into two groups: the experimental group (hydrogen-rich water treatment) and the control group (distilled water treatment). The rats consumed hydrogen-rich water or distilled water until 16 months of age. The experimental group exhibited lower periodontal oxidative damage at 16 months of age than the control group. Although protein expression of interleukin-1 beta did not differ, gene expression of Nod-like receptor protein 3 inflammasomes was activated in periodontal tissues from the experimental group as compared with the control group. Drinking hydrogen-rich water is proposed to have anti-aging effects on periodontal oxidative damage, but not on inflammatory reactions in healthy rats.

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  • 若年者における急性上気道炎の発症頻度と智歯萠出状態との関連

    町田 達哉, 水谷 慎介, 片岡 広太, 山根 真由, 丸山 貴之, 東 哲司, 友藤 孝明, 江國 大輔, 森田 学

    口腔衛生学会雑誌   64 ( 4 )   328 - 335   2014.7

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    口腔状態と気道感染の関連性の議論が近年盛んである。口腔は上気道と接しており、半萠出智歯の存在は、隣在歯との間に清掃困難な空間を形成し、智歯周囲の細菌量を増加させ、上気道を含む周囲組織に影響を与えうる。そこで、対象者を智歯萠出時期である18-20歳の大学生とし、急性上気道炎の発症頻度と智歯萠出状態の関連を検討することとした。2013年岡山大学新入生健康診断の際に歯科健診を任意受診した18-67歳(平均年齢18.5±2.1歳)の2,205名中、非喫煙者でデータ欠損のない18-20歳の1,964名(男性1,130名、女性834名)を分析対象とした。口腔診査を行い口腔清掃度、地域歯周疾患指数、および智歯萠出状態を調べた。自己記入式質問調査により過去1年間の急性上気道炎の発症頻度を尋ね、年間3回以上発症した群と3回未満発症した群の2群に分けた。χ2検定で2群比較を行い、有意な関連を智歯萠出状態と過去1年間の一番奥の歯ぐきの痛みの既往で認めた(P&lt;0.05)。口腔清掃度にも一定の関連性を認めた(P=0.054)。性別、口腔清掃度、智歯萠出状態、過去1年間の一番奥の歯ぐきの痛みの既往を説明変数としたロジスティック回帰分析を行ったところ、萠出智歯なしの者に対し、半萠出智歯を有する者は急性上気道炎を過去1年間に3回以上発症していた者の割合が有意に高かった(P=0.003)。大学生において智歯萠出状態と急性上気道炎の発症頻度に関連性があることが示唆された。(著者抄録)

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  • 頭頸部がん患者を支えるチーム医療 岡山大学病院頭頸部がんセンターにおける口腔ケア

    丸山 貴之, 志茂 加代子, 佐々木 禎子, 田中 千加, 横井 彩, 水谷 慎介, 山中 玲子, 森田 学

    口腔衛生学会雑誌   64 ( 3 )   316 - 316   2014.4

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  • 若年者における血圧と歯周炎との関連についてのコホート研究

    川端 勇也, 江國 大輔, 友藤 孝明, 東 哲司, 丸山 貴之, 水谷 慎介, 米田 俊樹, 町田 達哉, 片岡 広太, 山根 真由, 佐久本 雅美, 大森 智栄, 森田 学

    口腔衛生学会雑誌   64 ( 2 )   175 - 175   2014.4

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  • A simple test for salivary gland function measuring resting and stimulated submandibular and sublingual secretions. International journal

    Takashi Kanehira, Hirohisa Hongou, Kozo Asano, Manabu Morita, Etsuko Maeshima, Akemi Matsuda, Wataru Sakamoto

    Oral surgery, oral medicine, oral pathology and oral radiology   117 ( 2 )   197 - 203   2014.2

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    Objective. This study examined the application of a simple screening test for salivary gland function by measuring resting and stimulated submandibular and sublingual secretions.
    Study Design. An assay system was designed to use filter paper incorporating the chromophore of melanoidin or stimuli such as capsaicin and citric acid. We investigated the relationship between resting and stimulated secretions and melanoidin migration at 2 minutes for healthy and dry mouth groups.
    Results. The healthy group showed a significant increase in the migration of melanoidin in the paper after citric acid or capsaicin stimulation. In contrast, patients with Sjogren syndrome showed no significant migration in spite of the stimulation. However, some participants with Sjogren syndrome or dry mouth showed a significant increase in the migration of melanoidin after stimulation.
    Conclusions. These results show that the newly developed method should be useful for evaluation of residual salivary gland function and screening for hyposalivation with dry mouth.

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  • Anti-ageing effects of dentifrices containing anti-oxidative, anti-inflammatory, and anti-bacterial agents (Tomarina (R)) on gingival collagen degradation in rats

    Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Yasumasa Endo, Kenta Kasuyama, Tetsuji Azuma, Naofumi Tamaki, Toshiki Yoneda, Manabu Morita

    ARCHIVES OF ORAL BIOLOGY   59 ( 1 )   60 - 65   2014.1

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    Objective: Previous studies have demonstrated the relationship between ageing and oxidative stress. In this study, we examined the effects of topical application of a dentifrice containing anti-oxidative, anti-inflammatory, and anti-bacterial agents (Tomarina (R)) to the gingival surface on gingival collagen degradation in rats.
    Design: Fischer 344 male rats (4 or 8 months old) were divided into two groups: experimental group and control group. Tomarina (R) (the experimental group) or control dentifrice (the control group) was applied 5 days per week for 2 months.
    Results: In the control group, gingival collagen density decreased with ageing. In the experimental group, the collagen density did not change with ageing, and was greater than that in the control group at 10 months of age (p &lt; 0.0083). In addition, the control group showed an increase in serum oxidative stress with ageing. The experimental group also showed increased serum oxidative stress, but the value was lower than the control group at 10 months of age (p &lt; 0.0083). Furthermore, low expressions of protein oxidative damage in the periodontal tissue were observed in the experimental group, compared to the control group at 6 months and 10 months.
    Conclusion: These findings indicate that Tomarina (R) might suppress the effects of ageing on gingival collagen degradation, by decreasing oxidative stress in the rat model. (C) 2013 Elsevier Ltd. All rights reserved.

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  • Longitudinal Relationship between Plasma Reactive Oxygen Metabolites and Periodontal Condition in the Maintenance Phase of Periodontal Treatment

    Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Mayu Yamane, Toshiki Yoneda, Yuya Kawabata, Kota Kataoka, Naofumi Tamaki, Manabu Morita

    DISEASE MARKERS   2014   489292   2014

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    Aim. The present cohort study describes the longitudinal relationship between plasma oxidative status and periodontitis progression during the maintenance phase of treatment. Materials and Methods. Forty-five patients (mean age 58.8 years) were monitored from 2008 to 2013. Periodontal conditions, including probing pocket depth (PPD) and clinical attachment level (CAL), were recorded. Measurements of plasma reactive oxygen metabolites (ROM) and biologic antioxidant potential (BAP) were performed to evaluate plasma oxidative status. The patients were assigned into 2 groups as low and high plasma ROM level using a cut-off value which was median of plasma ROM level at baseline. Results. In the subjects with low plasma ROM level at baseline, changes in mean CAL were positively correlated with changes in plasma ROM levels, bleeding on probing, and plaque control record, but not with PPD. In the subjects with high plasma ROM at baseline, changes in CAL were significantly associated with only PPD at baseline. On the other hands there were no significant associations between changes in CAL and those in plasma BAP levels. Conclusions. When plasma ROM level in periodontitis patients was low, increases in plasma ROM level were associated with those in CAL during the maintenance phase of treatment.

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  • Periodontitis and liver disease

    Koichiro Irie, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    Studies on Periodontal Disease   197 - 210   2014

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  • Periodontitis and atherosclerosis

    Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    Studies on Periodontal Disease   183 - 196   2014

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  • Effect of periodontal therapy on circulating oxidative stress

    Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    Studies on Periodontal Disease   147 - 156   2014

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  • Association between oxidative stress and persiontal diseases in animal model studies

    Manabu Morita, Daisuke Ekuni, Takaaki Tomofuji

    Studies on Periodontal Disease   33 - 52   2014

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  • Structural equation modeling to assess gender differences in relationship between psychological symptoms and dental visit after dental checkup at university students

    Mizutani S, Ekuni D, Tomofuji T, Irie K, Azuma T, Iwasaki Y, Morita M

    Acta Odontol Scand   20   1 - 7   2014

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  • ライフステージに応じた歯周病予防

    歯界展望   123 ( 6 )   1206 - 1211   2014

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  • Effects of antioxidants on periodontal disease.

    Tomofuji T, Ekuni D, Mizutani S, Morita M

    Springer   2014

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  • 若年者における風邪の発症頻度と智歯の萠出状態との関連

    町田 達哉, 水谷 慎介, 片岡 広太, 山根 真由, 丸山 貴之, 友藤 孝明, 江國 大輔, 森田 学

    岡山歯学会雑誌   32 ( 2 )   87 - 87   2013.12

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  • アルコール摂取後の口腔保健行動の変化と歯周病との関連

    山根 真由, 江國 大輔, 水谷 慎介, 町田 達哉, 片岡 広大, 丸山 貴之, 森田 学

    岡山歯学会雑誌   32 ( 2 )   87 - 88   2013.12

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  • 頭頸部がん患者に対する歯科衛生士の取り組みと今後の展望

    佐々木 禎子, 志茂 加代子, 田中 千加, 三浦 留美, 山中 玲子, 丸山 貴之, 横井 彩, 水川 展吉, 森田 学, 宮脇 卓也

    岡山歯学会雑誌   32 ( 2 )   81 - 81   2013.12

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  • Relationships between self-rated oral health, subjective symptoms, oral health behavior and clinical conditions in Japanese university students: a cross-sectional survey at Okayama University. International journal

    Azusa Kojima, Daisuke Ekuni, Shinsuke Mizutani, Michiko Furuta, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

    BMC oral health   13 ( 1 )   62 - 62   2013.11

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    Background: Self-rated oral health is a valid and useful summary indicator of overall oral health status and quality of life. However, few studies on perception of oral health have been conducted among Japanese young adults. This study investigated whether oral health behavior, subjective oral symptoms, or clinical oral status were associated with self-rated oral health in Japanese young adults.
    Methods: This cross-sectional survey included 2,087 students (1,183 males, 904 females), aged 18 and 19 years, at Okayama University, Japan. A self-administered questionnaire was distributed and an oral examination was performed.
    Results: In a structural equation modeling analysis, the score of decayed, missing and filled teeth (DMFT) significantly affected self-rated oral health (p &lt;0.05) and the effect size was highest. Malocclusion, subjective symptoms of temporomandibular disorders (TMD) and stomatitis, and poor oral health behavior significantly induced self-rated poor oral health with small effect sizes (p &lt;0.05). Clinical periodontal conditions and Oral Hygiene Index-simplified were not related to self-rated oral health.
    Conclusion: Self-rated oral health was influenced by subjective symptoms of TMD and stomatitis, oral health behavior, the score of DMFT, and malocclusion. The evaluation of these parameters may be a useful approach in routine dental examination to improve self-rated oral health in university students.

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  • Occlusion and Weight Change in a Patient After Esophagectomy: Success Derived from Restoration of Occlusal Support

    Reiko Yamanaka, Yoshihiko Soga, Mami Minakuchi, Kumiko Nawachi, Takayuki Maruyama, Takuo Kuboki, Manabu Morita

    INTERNATIONAL JOURNAL OF PROSTHODONTICS   26 ( 6 )   574 - 576   2013.11

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    Occlusal support may be an important factor affecting nutritional support after major surgery. This report presents a patient who gained body weight after receiving a new prosthesis. The patient was an 82-year-old man with thoracic esophageal carcinoma. He did not have occlusal support because of multiple caries lesions. His body weight slowly increased after surgery, but almost stopped in the period of 54 to 68 days after surgery. After treatment with dentures (day 72 postsurgery), body weight gain was observed again, although his medical treatment had not changed. An appropriate prosthesis could contribute to perioperative nutrition support and may lead to earlier recovery after surgery.

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  • Effects of apoE deficiency and occlusal disharmony on amyloid-beta production and spatial memory in rats. International journal

    Daisuke Ekuni, Yasumasa Endo, Takaaki Tomofuji, Tetsuji Azuma, Koichiro Irie, Kenta Kasuyama, Manabu Morita

    PloS one   8 ( 9 )   e74966   2013.9

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    Amyloid-β (Aβ) plays a causative role in Alzheimer's disease. Apolipoprotein E (apoE) is involved in Aβ accumulation, whereas occlusal disharmony increases Aβ production in the rat hippocampus. The purpose of the present study was to investigate the effects of apoE deficiency and occlusal disharmony on Aβ production and spatial memory. Wild-type (WT) (n = 12) and apoE-deficient [ApoE(-/-)] (n = 12) rats (Sprague-Dawley
    8 weeks old) were used. These rats were randomly divided into four groups of six rats each: two control (C) groups: WT (C-WT) and ApoE [C-ApoE(-/-)], and two occlusal disharmony (D) groups: WT (D-WT) and ApoE [D-ApoE(-/-)]. The C group received no treatment for 8 weeks. In the D group, the maxillary molar cusps were cut off for 8 weeks. The spatial memory of rats was assessed according to their behavioral performance in a radial arm maze. In both genotypes of rats, significant differences in the reference memory, Aβ42 production, β-secretase expression and plasma corticosterone levels were observed between the C and D groups (P &lt
    0.0125). The levels of Aβ42 and glucocorticoid receptor in the C-ApoE(-/-) group were also significantly higher than those in the C-WT group (P &lt
    0.0125). However, no significant differences in these parameters were found between the two genotypes with occlusal disharmony. In conclusion, occlusal disharmony induces cognitive dysfunction and Aβ accumulation in the rat hippocampus, and the effects of occlusal disharmony on Aβ accumulation and cognitive dysfunction were larger than those of apoE deficiency. © 2013 Ekuni et al.

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  • Effects of eating behaviors on being overweight in japanese university students: a cross-sectional survey at the Okayama University. International journal

    Daisuke Ekuni, Michiko Furuta, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

    Asia-Pacific journal of public health   25 ( 4 )   326 - 34   2013.7

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    Being overweight is an important risk factor for lifestyle-related diseases. The objective of the present study was to examine whether eating until full, eating quickly, or both eating behaviors were associated with being overweight, defined as a body mass index (BMI) ≥ 25, in young adults. This cross-sectional survey comprised 1918 students (1139 male and 779 female students) at the Okayama University in Japan. In logistic regression analysis, eating until full was not associated with being overweight in either sex. The adjusted odds ratio of being overweight among those who reported eating quickly was 3.93 (2.45-6.31
    P &lt
    .0001) for male and 1.59 (0.79-3.21
    P =.193) for female students. Moreover, the adjusted odds ratio of being overweight was 2.72 (1.72-4.30
    P &lt
    .001) in male students who frequently reported eating a fatty diet. The combination of eating quickly and frequently eating a fatty diet had a supra-additive effect on being overweight in the male students. © 2011 APJPH.

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  • Effects of biliverdin administration on acute lung injury induced by hemorrhagic shock and resuscitation in rats. International journal

    Junko Kosaka, Hiroshi Morimatsu, Toru Takahashi, Hiroko Shimizu, Susumu Kawanishi, Emiko Omori, Yasumasa Endo, Naofumi Tamaki, Manabu Morita, Kiyoshi Morita

    PloS one   8 ( 5 )   e63606   2013.5

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    Hemorrhagic shock and resuscitation induces pulmonary inflammation that leads to acute lung injury. Biliverdin, a metabolite of heme catabolism, has been shown to have potent cytoprotective, anti-inflammatory, and anti-oxidant effects. This study aimed to examine the effects of intravenous biliverdin administration on lung injury induced by hemorrhagic shock and resuscitation in rats. Biliverdin or vehicle was administered to the rats 1 h before sham or hemorrhagic shock-inducing surgery. The sham-operated rats underwent all surgical procedures except bleeding. To induce hemorrhagic shock, rats were bled to achieve a mean arterial pressure of 30 mmHg that was maintained for 60 min, followed by resuscitation with shed blood. Histopathological changes in the lungs were evaluated by histopathological scoring analysis. Inflammatory gene expression was determined by Northern blot analysis, and oxidative DNA damage was assessed by measuring 8-hydroxy-2' deoxyguanosine levels in the lungs. Hemorrhagic shock and resuscitation resulted in prominent histopathological damage, including congestion, edema, cellular infiltration, and hemorrhage. Biliverdin administration prior to hemorrhagic shock and resuscitation significantly ameliorated these lung injuries as judged by histopathological improvement. After hemorrhagic shock and resuscitation, inflammatory gene expression of tumor necrosis factor-alpha and inducible nitric oxide synthase were increased by 18- and 8-fold, respectively. Inflammatory gene expression significantly decreased when biliverdin was administered prior to hemorrhagic shock and resuscitation. Moreover, after hemorrhagic shock and resuscitation, lung 8-hydroxy-2' deoxyguanosine levels in mitochondrial DNA expressed in the pulmonary interstitium increased by 1.5-fold. Biliverdin administration prior to hemorrhagic shock and resuscitation decreased mitochondrial 8-hydroxy-2' deoxyguanosine levels to almost the same level as that in the control animals. We also confirmed that biliverdin administration after hemorrhagic shock and resuscitation had protective effects on lung injury. Our findings suggest that biliverdin has a protective role, at least in part, against hemorrhagic shock and resuscitation-induced lung injury through anti-inflammatory and anti-oxidant mechanisms.

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  • Relationship between masticatory performance and heart rate variability: A pilot study

    Noriko Takeuchi, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

    ACTA ODONTOLOGICA SCANDINAVICA   71 ( 3-4 )   807 - 812   2013.5

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    Objective. Little information is available on how impaired masticatory performance relates to heart-rate variability (HRV). The aim of this study was to investigate the relationship between HRV indices and masticatory performance. Materials and methods. Sixty-five subjects (19 men and 46 women, 66.1 +/- 9.9 years old) who had received periodontal maintenance care at the Clinic of Preventive Dentistry of Okayama University Hospital were selected for the study. All subjects completed written questionnaires, a chewing (colour-changing gum) test for masticatory performance and measurement of occlusal force and HRV as well as oral examination. Results. The high sympathetic activity (LF &gt;= 49.6) group showed a significantly lower level of masticatory performance (a(star) value) than the low sympathetic activity group (p &lt; 0.05). There were also significant correlations of masticatory performance with LF and LF/HF (p &lt; 0.05). Conclusion. These findings suggest that there is relationship between masticatory performance and HRV indices and impaired masticatory performance may be a risk factor for inducing high sympathetic activity.

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  • Relationship between periodontal inflammation and fetal growth in pregnant women: a cross-sectional study. International journal

    Noriko Takeuchi, Daisuke Ekuni, Koichiro Irie, Michiko Furuta, Takaaki Tomofuji, Manabu Morita, Tatsuo Watanabe

    Archives of gynecology and obstetrics   287 ( 5 )   951 - 7   2013.5

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    Periodontal disease in pregnant women, evaluated by probing pocket depth (PPD) or clinical attachment level (CAL), significantly increases the risk of subsequent preterm birth (PB) and/or low birth weight (LBW). However, PPD and CAL do not always reflect current periodontal tissue inflammation. Therefore, assessment of bleeding on probing index (BOP), an indicator of periodontal inflammation, may be appropriate. The objective of this cross-sectional study was to investigate the relationship between periodontal inflammation and fetal growth in pregnant women after periodontal treatment.
    We recruited 203 pregnant women (mean age 31.8 +/- A 4.5 years), including 20 subjects with periodontal inflammation and 183 periodontally healthy controls, after periodontal treatment. Fetal growth parameters as well as periodontal conditions were recorded.
    Perinatal gestational age was 39.4 +/- A 1.3 weeks; 8 subjects had a PB and 12 had an infant with a LBW. Results of multiple stepwise regression analysis indicated that birth length was negatively correlated with BOP% (beta = -0.175; p = 0.002). Fetal femur length, and birth length and weight among mothers with low periodontal inflammation were significantly higher than those among mothers with high periodontal inflammation (p &lt; 0.05). The LBW rate in the high periodontal inflammation group was significantly higher than that in the low periodontal inflammation group (p &lt; 0.05).
    These results suggest that periodontal inflammation is correlated with fetal femur length, birth weight, and birth length.

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  • Effects of electrical stimulation on periodontal tissue remodeling in rats

    T. Tomofuji, D. Ekuni, T. Azuma, K. Irie, Y. Endo, K. Kasuyama, M. Nagayama, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   48 ( 2 )   177 - 183   2013.4

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    Background and Objective Electric current is used to promote wound healing. However, it is unclear whether electrical stimulation contributes to gingival tissue remodeling. This study examined the effects of electrical stimulation on gingival tissue remodeling in a rat periodontitis model. Material and Methods Male Wistar rats (n=28, 8wks of age) were divided into four groups of seven rats each. The control group did not receive any treatment for 6wks. In the other groups, periodontitis was ligature-induced for 4wks. After 4wks, the rats with periodontitis were given daily electrical stimulation of 0, 50 or 100A for 2wks. Results The periodontitis group stimulated with 0 A showed a higher density of polymorphonuclear leukocytes and a lower density of collagen in gingival tissue compared with the control group (p&lt;0.05). The two remaining groups treated with 50or 100A of electrical stimulation exhibited a lower density of polymorphonuclear leukocytes (p&lt;0.05) and a higher density of collagen than the group stimulated with 0A (p&lt;0.05). They also showed higher expression of fibroblast growth factor-2 than the group treated with 0A of electrical stimulation (p&lt;0.05). Conclusion Electric stimulation may offer a novel approach to promote gingival tissue remodeling in periodontal lesions.

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  • Self-reported aural symptoms, headache and temporomandibular disorders in Japanese young adults. International journal

    Rahena Akhter, Manabu Morita, Disuke Ekuni, Nur Mohammad Monsur Hassan, Michiko Furuta, Reiko Yamanaka, Yoshizo Matsuka, David Wilson

    BMC musculoskeletal disorders   14   58 - 58   2013.2

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    Background: To investigate the associations of aural symptoms, headache and depression with the presence of temporomandibular disorder (TMD) symptoms in a young adult population in Japan. Methods. A personal interview survey was conducted on first-year university students (n = 1,930) regarding symptoms of TMD, aural problems, headache, shoulder pain and depression. Logistic regression was applied to assess the associations of these problems with the presence of TMD symptoms after controlling for age and gender. Results: Among the 1,930 students, 543 students exhibited TMD symptoms and were classified into 7 groups: clicking only (Group I, n = 319), pain in the TMJ only (Group II, n = 21), difficulty in mouth opening only (Group III, n = 18), clicking and pain (Group IV, n = 29), clicking and difficulty in mouth opening (Group V, n = 48), difficulty in mouth opening and pain (Group VI, n = 11), and combination of three symptoms (Group VII, n = 97). The control group (n = 1,387) were subjects without any TMD symptoms. After adjusting for age and gender, a strong association was observed between TMD symptoms (Group II and IV) and tinnitus (OR = 12.1 and 13.2, respectively). TMD symptoms (Group I, II and III) were also associated with vertigo and headache. Otalgia and depression were significantly associated with the presence of clicking only. Conclusions: TMD symptoms were significantly correlated to aural symptoms and headache. A functional evaluation of the stomatognathic system should be considered in subjects with unexplained aural symptoms and headache. © 2013 Akhter et al.
    licensee BioMed Central Ltd.

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  • Preventive effects of trehalose on osteoclast differentiation in rat periodontitis model. International journal

    Yasumasa Endo, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Kenta Kasuyama, Manabu Morita

    Journal of clinical periodontology   40 ( 1 )   33 - 40   2013.1

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    Aim Trehalose, which is a disaccharide formed by a 1,1 linkage of two glucose molecules, was suggested to have a suppressive effect on bone resorption. In this study, we examined the effects of topical application of trehalose on osteoclast differentiation in a rat periodontitis model.
    Material and Methods Rats were divided into four groups. One group received no treatment. In the other groups, experimental periodontitis was induced by ligature placement. These rats with experimental periodontitis received topical application of pure water (vehicle group), 30 mg/ml trehalose solution (30 mg/ml trehalose group) or 60 mg/ml trehalose solution (60 mg/ml trehalose group) to the gingival sulcus respectively.
    Results The vehicle group showed higher numbers of polymorphonuclear leucocytes, receptor activator of nuclear factor kappa B ligand (RANKL)-positive cells and osteoclasts compared with the no treatment group respectively. Trehalose-applied groups exhibited lower numbers of these cells compared with the vehicle group. Gene expressions of tumour necrosis factor-a, RANKL and toll-like receptor 4 were suppressed by trehalose. In addition, protein expressions of RANKL inducing pathway were less activated by trehalose.
    Conclusion Topical application of trehalose could suppress osteoclast differentiation by inactivation of RANKL inducing pathway in the rat periodontitis model.

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  • Dental caries is correlated with knowledge of comprehensive food education in Japanese university students. International journal

    Daisuke Ekuni, Takaaki Tomofuji, Shinsuke Mizutani, Michiko Furuta, Koichiro Irie, Tetsuji Azuma, Azusa Kojima, Yoshiaki Iwasaki, Manabu Morita

    Asia Pacific journal of clinical nutrition   22 ( 2 )   312 - 8   2013

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    In Japan, the "Food Education Basic Law" (The Basic Law on Shokuiku, food education) was enacted in 2005. The comprehensive food education programs, namely Shokuiku, aim to improve dietary practices to reduce lifestyle-related diseases. Dental caries is one of the diseases associated with inappropriate dietary habits. Thus, food education may influence the prevalence of dental caries. However, there are no data regarding the association between public based-food education and dental caries. The aim of the present study was to investigate the relationship between knowledge about comprehensive food education (Shokuika) and dental caries experience in Japanese university students. A total of 2,184 students (1,240 men, 944 women), aged 18-20 years, were examined. They had attended the Shokuiku program while in junior/senior high school. The numbers of teeth present, and decayed, missing and filled teeth (DMFT) (dental caries experience) were recorded. Additional information was collected via a questionnaire regarding knowledge about food education, dietary habits and oral health behavior. Of the students, 315 men (20.7%) and 345 women (52.8%) reported that they know and can explain the meaning and content of the word "Shokuiku". After adjusting for potential confounding factors, subjects who did not have knowledge about Shokuiku had higher adjusted odds ratio (OR) for dental caries experience (DMFT &gt;0) than those who had (adjusted OR, 1.23; 95% CI, 1.02-1.48; p&lt;0.05). These observations revealed that having knowledge about comprehensive food education in university students correlates with low prevalence of dental caries.

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  • 頭頸部がん患者に対して口腔ケアを行った2症例

    日本歯周病学会雑誌   55 ( 3 )   262 - 268   2013

  • ライフステージに応じた歯周病対策の提案

    日本歯科医師会雑誌   66   21 - 30   2013

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  • 頭頸部がん患者における予後推定栄養指数と口腔内状態との関係

    口腔衛生学会雑誌   63   321 - 327   2013

  • 実物大臓器立体モデルを活用した手術支援の実際と歯科技工士の関わり

    竹内哲男, 有地秀裕, 松本 洋, 中原龍一, 水川展吉, 吉岡徳枝, 木股敬裕, 尾崎敏文, 飯田征二, 佐々木朗, 三野卓哉, 前川賢治, 森田 学, 槇野博史, 窪木拓男

    QDT別冊 CAD/CAM YEAR BOOK 2013   別冊   22 - 31   2013

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  • Gum chewing modulates heart rate variability under noise stress. International journal

    Daisuke Ekuni, Takaaki Tomofuji, Noriko Takeuchi, Manabu Morita

    Acta odontologica Scandinavica   70 ( 6 )   491 - 6   2012.12

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    Objective. Gum chewing may relieve stress, although this hypothesis has not been proven. Heart-rate variability (HRV) is commonly used to measure stress levels. However, it is not known if gum chewing modulates HRV under acute stress. The aim of this study was to investigate the effects of gum chewing on HRV under acute stress. Materials and methods: A crossover study involving 47 non-smoking healthy subjects, aged 22-27 years, was carried out. The subjects received a stress procedure with gum chewing (GS group) and without gum chewing (S group). Additionally, the other 20 subjects were allocated to the gum chewing without stress group (G group). The GS and S groups were exposed to noise for 5 min (75 dBA) as stress. Before and after stress exposure/gum chewing, participants completed the state portion of the State-Trait Anxiety Inventory (STAI-s) and a single Stress Visual Analogue Scale (VAS) measurement. HRV measurement was performed before and during stress/gum chewing for 5 min. Results. After the stress procedure, VAS score significantly increased in the GS and S groups. During the stress procedure, the GS group showed a significantly lower level of high frequency (HF) and higher levels of low frequency (LF) and LF/HF than the S group. However, there were no significant differences in the scores of the state portion of the State-Trait Anxiety Inventory (STAI-s) and VAS between the two stress groups. Conclusions. These findings suggest that gum chewing modulates HRV, but may not relieve acute stress caused by noise.

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  • Hydrogen-rich water prevents lipid deposition in the descending aorta in a rat periodontitis model. International journal

    Daisuke Ekuni, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Shinsuke Mizutani, Azusa Kojima, Manabu Morita

    Archives of oral biology   57 ( 12 )   1615 - 22   2012.12

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    Objective: Periodontitis has been causally linked to atherosclerosis, which is mediated by the oxidative stress. As hydrogen-rich water (HW) scavenges reactive oxygen species (ROS), we hypothesized that HW could prevent lipid deposition induced by periodontitis in the aorta. The aim of this study was to investigate the effects of HW on the initiation of atherosclerosis in a rat periodontitis model.
    Design: Eighteen 8-wk-old male Wistar rats were divided into three groups of six rats; the periodontitis group, periodontitis + HW group and the no treatment (control) group. In the periodontitis and periodontitis + HW groups, periodontitis was induced using a ligature for 4 wk, while the periodontitis + HW group was given water containing 800-1000 mu g/L hydrogen during the 4-wk experimental period.
    Results: In the periodontitis group, lipid deposition in the descending aorta was observed. The periodontitis group also showed significant higher serum levels for ROS and oxidised low-density lipoprotein-cholesterol (ox-LDL) (1.7 and 1.4 times, respectively), and higher aortic expression levels of nitrotyrosine and hexanoyl-lysine (HEL) (7.9 and 16.0 times, respectively), as compared to the control group (p &lt; 0.05). In the periodontitis + HW group, lipid deposition was lower. Lower serum levels of ROS and ox-LDL (0.46 and 0.82 times, respectively) and lower aortic levels of nitrotyrosine and HEL (0.27 and 0.19 times, respectively) were observed in the periodontitis + HW group than in the periodontitis group (p &lt; 0.05).
    Conclusions: HW intake may prevent lipid deposition in the rat aorta induced by periodontitis by decreasing serum ox-LDL levels and aortic oxidative stress. (C) 2012 Elsevier Ltd. All rights reserved.

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  • 頭頸部がんセンターにおける定期的な病棟往診の役割

    丸山 貴之, 志茂 加代子, 佐々木 禎子, 水谷 慎介, 児島 梓, 米田 俊樹, 町田 達哉, 横井 彩, 山中 玲子, 森田 学

    岡山歯学会雑誌   31 ( 2 )   91 - 92   2012.12

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  • Relationship between serum albumin concentration and periodontal condition in patients with head and neck cancer. International journal

    Takayuki Maruyama, Reiko Yamanaka, Aya Yokoi, Daisuke Ekuni, Takaaki Tomofuji, Nobuyoshi Mizukawa, Tomoo Onoda, Motoharu Eguchi, Manabu Morita

    Journal of periodontology   83 ( 9 )   1110 - 5   2012.9

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    Background: Serum albumin concentration is known to be an independent predictor of survival in head and neck cancer. The previous studies suggested relationships between serum albumin concentration and oral health status in populations without serious systemic disorders. However, these relationships remain unclear in patients with head and neck cancer. The purpose of this study is to investigate the relationship between serum albumin concentration and oral health status in patients with head and neck cancer.
    Methods: Fifty individuals diagnosed with primary head and neck cancer, 25 individuals with normal serum albumin concentration (&gt;= 3.85 g/dL), and 25 age- and sex-matched individuals with lower serum albumin concentration (&lt;3.85 g/dL) were analyzed. General status, including cancer stage, body mass index, drinking and smoking habits, and biochemical serum markers, were evaluated. Oral health status, including periodontal condition and occlusion tooth pairs, were also evaluated.
    Results: Mean clinical attachment level (CAL) and C-reactive protein in the lower serum albumin concentration group were greater than those in the normal serum albumin concentration group (P = 0.009 and P = 0.002, respectively). However, there were no significant differences in any other oral and/or serum parameters between the two serum albumin groups. A logistic regression model showed that mean CAL was significantly associated with high or low levels of serum albumin concentration (odds ratio = 9.752; 95% confidence interval = 1.702 to 55.861; P = 0.011).
    Conclusions: This study suggests an association between periodontal disease and serum albumin concentration in patients with head and neck cancer. Longitudinal studies are necessary to examine the causal relationship between serum albumin concentration and periodontal condition. J Periodontol 2012;83:1110-1115.

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  • Effects of self-efficacy on oral health behaviours and gingival health in university students aged 18- or 19-years-old. International journal

    Shinsuke Mizutani, Daisuke Ekuni, Michiko Furuta, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Azusa Kojima, Jun Nagase, Yoshiaki Iwasaki, Manabu Morita

    Journal of clinical periodontology   39 ( 9 )   844 - 9   2012.9

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    Aim Although self-efficacy is known to affect various health-related practises, few studies have clearly examined how self-efficacy correlates with oral health behaviors or the oral health condition. We examined the relationship between gingivitis, oral health behaviors and self-efficacy in university students. Material & Methods A total of 2,111 students (1,197 males, 914 females) aged 18 and 19 years were examined. The degree of gingivitis was expressed as the percentage of bleeding on probing (%BOP). Additional information was collected via a questionnaire regarding oral health behaviors (daily frequency of tooth-brushing, use of dental floss and regular check-up). Self-efficacy was assessed using the Self-Efficacy Scale for Self-care (SESS). Path analysis was used to test pathways from self-efficacy to oral health behaviors and %BOP. Results In the final structural model, self-efficacies were related to each other, and they affected oral health behaviors. Good oral health behaviors reduced dental plaque and calculus, and lower levels of dental plaque and calculus resulted in lower %BOP. Conclusion Higher self-efficacy correlated with better oral health behaviours and gingival health in university students. Improving self-efficacy may be beneficial for maintaining good gingival health in university students. To prevent gingivitis, the approach of enhancing self-efficacy in university students would be useful.

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  • Hydrogen-rich water prevents progression of nonalcoholic steatohepatitis and accompanying hepatocarcinogenesis in mice. International journal

    Daisuke Kawai, Akinobu Takaki, Atsuko Nakatsuka, Jun Wada, Naofumi Tamaki, Tetsuya Yasunaka, Kazuko Koike, Ryuichiro Tsuzaki, Kazuyuki Matsumoto, Yasuhiro Miyake, Hidenori Shiraha, Manabu Morita, Hirofumi Makino, Kazuhide Yamamoto

    Hepatology (Baltimore, Md.)   56 ( 3 )   912 - 21   2012.9

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    Oxidative stress is a strong contributor to the progression from simple fatty liver to nonalcoholic steatohepatitis (NASH). Molecular hydrogen is an effective antioxidant that reduces cytotoxic reactive oxygen species. In this study, we investigated the effects of hydrogen-rich water and the drug pioglitazone on the progression of NASH in mouse models. A methionine-cholinedeficient (MCD) diet mouse model was prepared. Mice were divided into three experimental groups and fed for 8 weeks as follows: (1) MCD diet + control water (CW group); (2) MCD diet + hydrogen-rich water (HW group); and (3) MCD diet mixed with pioglitazone (PGZ group). Plasma alanine aminotransferase levels, hepatic expression of tumor necrosis factor-a, interleukin-6, fatty acid synthesisrelated genes, oxidative stress biomarker 8-hydroxydeoxyguanosine (8-OHdG), and apoptosis marker terminal deoxynucleotidyl transferasemediated deoxyuridine triphosphate nick-end labeling (TUNEL)positive cells in the liver were decreased in the HW and PGZ groups. The HW group showed a smaller decrease in hepatic cholesterol; however, stronger antioxidative effects in serum and lower peroxisome proliferator-activated receptor-a expression in the liver were seen in comparison with the PGZ group. We then investigated the effects of hydrogen in the prevention of hepatocarcinogenesis in STAM mice, known as the NASH-related hepatocarcinogenesis model. Eight-week-old male STAM mice were divided into three experimental groups as follows: (1) control water (CW-STAM); (2) hydrogen-rich water (HW-STAM); and (3) pioglitazone (PGZ-STAM). After 8 weeks, hepatic tumors were evaluated. The number of tumors was significantly lower in the HW-STAM and PGZ-STAM groups than in the CW-STAM group. The maximum tumor size was smaller in the HW-STAM group than in the other groups. Conclusion: Consumption of hydrogen-rich water may be an effective treatment for NASH by reducing hepatic oxidative stress, apoptosis, inflammation, and hepatocarcinogenesis. (HEPATOLOGY 2012;56:912921)

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  • Streptozotocin投与NASH発癌モデルマウスにおける病態進行と水素水投与の有効性の検討

    河合 大介, 高木 章乃夫, 山本 和秀, 中司 敦子, 和田 淳, 玉木 直文, 安中 哲也, 小池 和子, 津崎 龍一郎, 松本 和幸, 三宅 康広, 白羽 英則, 森田 学, 槇野 博史

    日本消化器病学会雑誌   109 ( 臨増大会 )   A708 - A708   2012.9

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  • Community-level socioeconomic status and parental smoking in Japan. International journal

    Kenji Takeuchi, Jun Aida, Manabu Morita, Yuichi Ando, Ken Osaka

    Social science & medicine (1982)   75 ( 4 )   747 - 51   2012.8

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    Community-level social environment has been considered to be associated with smoking behavior. However, no study has examined the association between community-level environmental factors and parental smoking behavior in families with young children. The aim of the present study was to examine the association between community-level socioeconomic status (SES) and parental smoking behavior. We used data from a cross-sectional study conducted from 2005 to 2006. We randomly selected 44 Japanese municipalities, 39 of which municipalities agreed to participate in this survey. The study subjects were participants in health check-ups for three-year-old children. Smoking status and individual demographic characteristics were obtained using self-administered questionnaires. Community-level variables were obtained from national census data for 2005. The prevalence of employment in tertiary industries and of unemployment was used to measure community-level SES. Multilevel Poisson regression models were used to calculate prevalence ratios (PRs) for smoking. Of 4143 subjects, a total of 3301 parents in 39 municipalities participated in our survey. Among the 2975 participants (71.8%) included in our analysis, 59.0% were smokers. There was no association between the job of the head of the household considered as an indicator of individual-level SES and smoking. By contrast, when we examined the relationship between prevalence of employment in tertiary industries as community-level SES and smoking, parents living in low middle SES municipalities had a significantly higher prevalence ratio for smoking, compared to parents living in the highest SES municipalities. This result suggested that those with lower community-level SES tended to have a higher prevalence of parental smoking regardless of individual-level SES. (C) 2012 Elsevier Ltd. All rights reserved.

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  • Self-reports of eating quickly are related to a decreased number of chews until first swallow, total number of chews, and total duration of chewing in young people. International journal

    Daisuke Ekuni, Michiko Furuta, Noriko Takeuchi, Takaaki Tomofuji, Manabu Morita

    Archives of oral biology   57 ( 7 )   981 - 6   2012.7

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    Objective: The validity of a self-questionnaire about eating quickly remains unclear. If a significant relationship between subjective and objective methods to evaluate eating quickly can be confirmed, then the subjective method can be widely and reliably used in many fields. This study investigated relationships between subjective and objective methods to evaluate eating quickly and also numerically characterized the kinesis of eating quickly in young people.
    Design: One hundred and thirteen students (44 males and 69 females; mean age 22.8 +/- 2.0 years) were selected. All subjects completed written questionnaires, and number of chews until first swallow, total duration of chewing, number of chews, chewing rate and bite size were measured using test products (a Japanese cracker and rice ball).
    Results: Both male and female subjects who reported eating quickly showed a significantly lower number of chews until first swallow (Japanese cracker), a lower number of chews overall (rice ball), and a shorter total duration of chewing (rice ball) than other subjects. There was no difference in chewing rate between subjects who ate quickly or not.
    Conclusions: These findings suggest that using test products, self-reports of eating quickly are related to a decreased number of chews until first swallow, total number of chews, and total duration of chewing, but not chewing rate, and that a self-reported questionnaire to evaluate eating rate is valid in young people. (C) 2012 Elsevier Ltd. All rights reserved.

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  • 耳鼻咽喉科病棟看護師を対象に口腔ケアに関するアンケートの実施とその報告

    志茂 加代子, 岡崎 惠子, 三浦 留美, 山中 玲子, 水川 展吉, 江国 大輔, 丸山 貴之, 横井 彩, 仲田 直樹, 武田 斉子, 喜田 沙音里, 森田 学

    岡山歯学会雑誌   31 ( 1 )   34 - 35   2012.6

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  • 小学生における永久歯の萠出状況の報告

    横井 彩, 山中 玲子, 江國 大輔, 丸山 貴之, 入江 浩一郎, 遠藤 康正, 水谷 慎介, 友藤 孝明, 森田 学

    岡山歯学会雑誌   31 ( 1 )   39 - 40   2012.6

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  • Social capital and self-rated oral health among young people. International journal

    Michiko Furuta, Daisuke Ekuni, Soshi Takao, Etsuji Suzuki, Manabu Morita, Ichiro Kawachi

    Community dentistry and oral epidemiology   40 ( 2 )   97 - 104   2012.4

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    Objectives: A few studies have revealed the impact of neighborhood social capital on oral health among young people. We sought to examine the associations of social capital in three settings (families, neighborhoods, and schools) with self-rated oral health among a sample of college students in Japan. Methods: Cross-sectional survey of 967 students in Okayama University, aged 18 and 19 years, was carried out. Logistic regression was used to examine the associations of poor self-rated oral health with perceptions of social capital, adjusting for self-perceived household income category and oral health behaviors. Results: The prevalence of subjects with poor self-rated oral health was 22%. Adjusted for gender, self-perceived household income category, dental fear, toothbrush frequency, and dental floss use, poor self-rated oral health was significantly associated with lower level of neighborhood trust [odds ratio (OR) 2.22; 95% confidence interval (CI): 1.40-3.54] and lower level of vertical trust in school (OR 1.71; 95% CI: 1.05-2.80). Low informal social control was unexpectedly associated with better oral health (OR 0.54; 95% CI: 0.34-0.85). Conclusion: The association of social capital with self-rated oral health is not uniform. Higher trust is associated with better oral health, whereas higher informal control in the community is associated with worse oral health.

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  • Effects of topical application of inorganic polyphosphate on tissue remodeling in rat inflamed gingiva

    K. Kasuyama, T. Tomofuji, D. Ekuni, T. Azuma, K. Irie, Y. Endo, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   47 ( 2 )   159 - 164   2012.4

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    Background and Objective: Inorganic polyphosphate [poly(P)] is a biopolymer found in almost all cells and tissues, and which promotes tissue remodeling. However, there is limited information on how poly(P) affects the connective tissue in inflamed gingiva. This study examined the effects of topical application of poly(P) on gingival connective tissue and its remodeling in a rat periodontitis model.
    Material and Methods: Male Wistar rats (n = 36, 8 wk of age) were used in this 6-wk study. The rats were divided into six groups of six rats each. The control group received no treatment. In the other groups, periodontitis was ligatureinduced for 4 wk. After 4 wk, the rats with periodontitis were further divided into five groups, and were left untreated (periodontitis group) or subjected to topical application of oral rinses containing 0, 0.1, 1 or 5% poly(P) for 2 wk.
    Results: The periodontitis and 0% poly(P) groups showed a higher density of polymorphonuclear leukocytes and a lower density of collagen in gingival tissue than the control group (p &lt; 0.05). In contrast, groups treated with more than 1% poly(P) exhibited a lower density of polymorphonuclear leukocytes (p &lt; 0.05) and a higher density of collagen than the periodontitis and 0% poly(P) groups (p &lt; 0.05). A higher expression of fibroblast growth factor-2 was observed in the gingiva of rats treated with 1% poly(P) than in those treated with 0% poly(P) (p &lt; 0.05).
    Conclusion: Topical application of poly(P) may induce connective tissue remodeling, contributing to improvement of inflamed gingiva in rats.

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  • Combined effects of hydrogen sulfide and lipopolysaccharide on osteoclast differentiation in rats. International journal

    Koichiro Irie, Daisuke Ekuni, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Ken Yaegaki, Manabu Morita

    Journal of periodontology   83 ( 4 )   522 - 7   2012.4

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    Background: Lipopolysaccharide (LPS) stimulates osteoclast differentiation through toll-like receptors (TLRs) 2 and 4, and hydrogen sulfide (H2S) induces osteoclast differentiation. If H2S activates TLRs, H2S may enhance the effects of LPS on osteoclast differentiation. The purpose of the present study is to examine the combined effects of sodium hydrogen sulfide (NaHS, an H2S donor drug) and LPS on osteoclast differentiation and TLR expression in rat periodontal tissue.
    Methods: Twenty-eight male Wistar rats (8 weeks old) were divided into four groups (n = 7 per group): a control (no treatment) group and three experimental groups (NaHS group, LPS group, and a combination [NaHS + LPS] group). At 1 day after topical application of NaHS and/or Porphyromonas gingivalis LPS into the gingival sulcus of first molars, the number of tartrate-resistant acid phosphate (TRAP)-positive osteoclasts in the periodontal tissue was counted. Expression of TLR2 and TLR4 mRNAs and proteins in the gingival was also assessed.
    Results: The number of TRAP-positive osteoclasts was significantly higher in the combination group than in any other group (P &lt; 0.01). The combination group had 11.0-fold higher TLR4 mRNA levels than the control group. TLR4 protein levels were also higher in the combination group than in the NaHS or LPS group. However, the TLR2 mRNA and protein levels were not significantly different in the combination group and the LPS group.
    Conclusion: In rat periodontal tissue, NaHS and LPS had an additive effect on osteoclast differentiation through activation of the TLR4 pathway but not the TLR2 pathway. J Periodontol 2012;83:522-527.

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  • Supplementation of broccoli or Bifidobacterium longum-fermented broccoli suppresses serum lipid peroxidation and osteoclast differentiation on alveolar bone surface in rats fed a high-cholesterol diet. International journal

    Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Tatsuo Yamamoto, Atsushi Ishikado, Takehiko Sato, Kayo Harada, Hirohisa Suido, Manabu Morita

    Nutrition research (New York, N.Y.)   32 ( 4 )   301 - 7   2012.4

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    High-cholesterol diet enhances osteoclastic activity on alveolar bone by increasing serum lipid peroxidation. We hypothesized that supplementation with dietary antioxidants, such as found in broccoli and its fermented products, might suppress increases in serum lipid peroxidation, contributing to the inhibition of osteoclastic activity after high-cholesterol diet intake. The purpose of the present study was to investigate the effects of broccoli and fermented broccoli consumption on serum lipid peroxidation and osteoclast differentiation in alveolar bone of rats fed a high-cholesterol diet. In this 12-week study, rats were divided into 4 groups (n = 6/group): a control group (fed regular diet) and 3 experimental groups (fed a high-cholesterol [1% wt/wt] diet, or a high-cholesterol diet supplemented with either broccoli powder [5% wt/wt] or Bifidobacterium longum-fermented broccoli powder [5% wt/wt]). Serum hexanoyl-lysine (HEL) levels were measured as a parameter of lipid peroxidation. The number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts in alveolar bone was enumerated to evaluate osteoclast differentiation. When compared with regular diet, the high-cholesterol diet increased serum HEL levels and resulted in a higher number of TRAP-positive osteoclasts at 12 weeks. The high-cholesterol diet supplemented with broccoli or B. longum-fermented broccoli showed lower levels of serum HEL and fewer TRAP-positive osteoclasts than the high-cholesterol diet at 12 weeks. In conclusion, consumption of broccoli, or its fermented product, inhibited the effects of a high-cholesterol diet on osteoclast differentiation in rat alveolar bone by suppressing serum lipid peroxidation. (C) 2012 Elsevier Inc. All rights reserved.

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  • Toll like receptor 5 ligand induces monocyte chemoattractant protein-1 in mouse osteoblastic cells.

    Kimiya Nakamura, Yoshiaki Deyama, Yoshitaka Yoshimura, Kuniaki Suzuki, Manabu Morita

    Biomedical research (Tokyo, Japan)   33 ( 1 )   39 - 44   2012.2

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    Flagellin, the ligand of Toll like receptor 5, is the major subunit of bacterial flagella. Flagellin stimulates various cells to release chemokines. Monocyte chemoattractant protein-1 (MCP-1) is a member of the CC chemokine family that is involved in monocyte infiltration in inflammatory diseases. It has been reported that serum MCP-1 levels increase proportionally with the severity of periodontal disease. Inflammatory mediators induce MCP-1 production in various cells, including osteoblasts. However, it remains unclear whether MCP-1 is released from osteoblasts in response to flagellin. In the present study, we investigated the effects of flagellin on the expression of MCP-1 in the mouse osteoblastic cell line, MC3T3-E1 (El) cells. Flagellin markedly increased MCP-1 mRNA level in a dose-dependent manner. The effect of flagellin on MCP-1 mRNA expression in El cells was transient, with a peak at 1 h. Concomitant with MCP-1 mRNA expression, MCP-1 protein levels were clearly elevated at 3 h after flagellin exposure. In addition, we revealed that INK and MEK-ERK1/2 are involved in flagellin-induced MCP-1 expression in El cells. These results indicated that bacterial flagellin may play an important role in the progression of periodontitis. Results of further studies will provide more clues to the prevention of periodontal diseases.

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  • 大学新入生の口腔健康状態の調査(性差および経年的変化の検討)

    児島 梓, 江國大輔, 古田美智子, 入江浩一郎, 東 哲司, 水谷慎介, 山中玲子, 友藤孝明, 森田 学, 山本龍生, 村上 隆, 山城 隆, 古賀 光, 岩﨑良章, 清水幸登, 大西 勝, 小倉俊郎

    Campus Health   49 ( 3 )   91 - 96   2012

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  • 生涯を通じての歯周病対策 -セルフケア,プロフェッショナルケア,コミュニティケア-

    森田 学, 稲垣幸司, 王 宝禮, 埴岡 隆, 藤井健男, 両角俊哉, 伊藤 弘, 山本龍生, 吉江弘正

    日本歯周病学会雑誌   54 ( 4 )   352 - 374   2012

  • 岡山大学病院歯科系診療科等が医科系診療科等から受けた院内紹介とそれに対する初期対応

    曽我賢彦, 蔵重恵美子, 山中玲子, 吉富愛子, 森田 学

    岡山歯学会雑誌   31 ( 2 )   67 - 72   2012

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  • 岡山大学新入生におけるう蝕経験の有無と食生活に対する自己効力感との関係.

    水谷慎介, 江國大輔, 古田美智子, 友藤孝明, 入江浩一郎, 東 哲司, 児島 梓, 長瀬 純, 森田 学

    口腔衛生学会雑誌   62 ( 4 )   403 - 409   2012

  • 早食いの是正に対するパンフレットと食事記録法の効果

    口腔衛生学会雑誌   62 ( 4 )   403 - 409   2012

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  • 障害者の生涯にわたる口腔のQOL向上を目的とした地域連携クリニカルパスの開発.

    岡山歯学会雑誌   31 ( 1 )   1 - 13   2012

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  • 歯科医師卒後臨床研修におけるチーム医療研修プログラム -研修歯科医のに関心および理解に与えた効果の検討-

    山中玲子, 曽我賢彦, 吉冨愛子, 白井肇, 鈴木康司, 河野隆幸, 鳥井康弘, 森田学

    歯科医学教育学会雑誌   28 ( 2 )   76 - 84   2012

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  • 市町村における歯科保健事業と精神及び行動の障害に関する医療費との関連.

    竹内倫子, 山本龍生, 森田 学

    日本口腔衛生会誌   62 ( 3 )   403 - 409   2012

  • Development of the outcome expectancy scale for self-care among periodontal disease patients. International journal

    Naoki Kakudate, Manabu Morita, Shunichi Fukuhara, Makoto Sugai, Masato Nagayama, Emiko Isogai, Masamitsu Kawanami, Itsuo Chiba

    Journal of evaluation in clinical practice   17 ( 6 )   1023 - 9   2011.12

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    Rationale The theory of self-efficacy states that specific efficacy expectations affect behaviour. Two types of efficacy expectations are described within the theory. Self-efficacy expectations are the beliefs in the capacity to perform a specific behaviour. Outcome expectations are the beliefs that carrying out a specific behaviour will lead to a desired outcome.
    Objective To develop and examine the reliability and validity of an outcome expectancy scale for self-care (OESS) among periodontal disease patients.
    Methods A 34-item scale was tested on 101 patients at a dental clinic. Accuracy was improved by item analysis, and internal consistency and test-retest stability were investigated. Concurrent validity was tested by examining associations of the OESS score with the self-efficacy scale for self-care (SESS) score and plaque index score. Construct validity was examined by comparing OESS scores between periodontal patients at initial visit (group 1) and those continuing maintenance care (group 2).
    Results Item analysis identified 13 items for the OESS. Factor analysis extracted three factors: social-, oral- and self-evaluative outcome expectancy. Cronbach&apos;s alpha coefficient for the OESS was 0.90. A significant association was observed between test and retest scores, and between the OESS and SESS and plaque index scores. Further, group 2 had a significantly higher mean OESS score than group I.
    Conclusion We developed a 13-item OESS with high reliability and validity which may be used to assess outcome expectancy for self-care. A patient&apos;s psychological condition with regard to behaviour and affective status can be accurately evaluated using the OESS with SESS.

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  • 小学生における永久歯の萠出状況の報告

    横井 彩, 山中 玲子, 江國 大輔, 丸山 貴之, 入江 浩一郎, 遠藤 康正, 水谷 慎介, 友藤 孝明, 森田 学

    岡山歯学会雑誌   30 ( 2 )   87 - 87   2011.12

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  • 耳鼻咽喉科病棟看護師を対象に口腔ケアに関するアンケートの実施とその報告

    志茂 加代子, 岡崎 惠子, 三浦 留美, 山中 玲子, 水川 展吉, 江国 大輔, 丸山 貴之, 横井 彩, 仲田 直樹, 武田 斉子, 喜田 沙音里, 森田 学

    岡山歯学会雑誌   30 ( 2 )   82 - 82   2011.12

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  • Relationship between impacts attributed to malocclusion and psychological stress in young Japanese adults. International journal

    Daisuke Ekuni, Michiko Furuta, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Takashi Murakami, Takashi Yamashiro, Toshio Ogura, Manabu Morita

    European journal of orthodontics   33 ( 5 )   558 - 63   2011.10

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    Identifying risk factors is important to prevent a wide range of health-damaging behaviours and to improve the quality of life of young people. The aim of this study was to investigate the relationship between impacts on daily performance attributed to malocclusion and psychological stress in healthy young Japanese adults.
    Medical and oral health data were collected during a cross-sectional examination conducted by the Health Service Center of Okayama University. Systemically healthy non-smoking students aged 18 and 19 years (n = 641; 329 males and 312 females) were included. Malocclusion was defined using a modified version of the Index of Orthodontic Treatment Need (IOTN). The impacts on daily performance attributed to malocclusion and psychological stress were assessed using self-reported questionnaires, the condition-specific oral impacts on daily performances (CS-OIDP), and the Hopkins Symptoms Checklist. Mann-Whitney U- and chi-square tests and structural equation modelling (SEM) were used for statistical analysis.
    Forty per cent of subjects had a malocclusion (n = 255). Subjects with impacts on daily performance had a significantly higher prevalence of malocclusion than those without impacts (P &lt; 0.001). SEM showed that psychological stress, especially interpersonal sensitivity and depression, was significantly correlated with CS-OIDP and malocclusion. Negative impacts on daily performance attributed to malocclusion may contribute to psychological stress in young Japanese adults.

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  • 小学生における視力と口唇閉鎖力の関係(Relationship between Visual Acuity and Labial Closure Force in Japanese Elementary School Children)

    山中 玲子, アクター・ラヘナ, 古田 美智子, 江國 大輔, 江崎 光恵, 山本 龍生, 岡崎 好秀, 丸山 貴之, 横井 彩, 森田 学

    口腔衛生学会雑誌   61 ( 3 )   288 - 294   2011.7

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    口唇閉鎖力は、主に口輪筋の機能を反映している。口輪筋は発音や咀嚼などさまざまな口腔機能に関連している。口唇閉鎖力は咀嚼能力や不正咬合に関連がある。一方、裸眼視力1.0未満(&lt;20/20)の小学生は、日本においてこの20年間増え続けている。小学生の視力低下を予防することは、重要な課題である。視力は眼輪筋の強さに関連する可能性がある。眼輪筋は口輪筋とともに顔面筋の一つであり、両者ともに顔面神経の支配を受けており、頬筋などを介して機械的にもつながっており、協調して運動する。口輪筋の強さは、眼輪筋の強さを反映していると推測される。本研究では、口唇閉鎖力が視力に関連しているという仮説を立て、小学生の視力と口唇閉鎖力の関連性を横断的に検討することを目的とした。対象は岡山市内の7歳から12歳までの小学生396名(男子197名、女子199名)とした。口腔内状態と視力は、定期健康診断の結果を用いた。口唇閉鎖力は、LIP DE CUM/LDC-110(コスモ計器、東京)を用いて測定した。典型的な不正咬合を有する16名を除外し、高視力群(≧20/20)264名と低視力群(&lt;20/20)116名に分けた。統計分析には、Mann-Whitney U検定、χ2検定、ロジスティック回帰分析を用いた。高視力群では、口唇閉鎖力(p=0.024)と男子の割合(p=0.045)が有意に高く、年齢(p=0.001)とヘルマンの歯齢(p=0.002)が有意に低かった。性別、年齢、ヘルマンの歯齢で調整しても、低視力群では口唇閉鎖力が有意に低かった(OR 1.65、95% CI 1.04-2.64、p=0.004)。小学生において、視力と口唇閉鎖力には関連があることが示唆された。(著者抄録)

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  • 頭頸部癌患者における口腔内状態と血清アルブミン値の関連

    山中 玲子, 丸山 貴之, 横井 彩, 森田 学, 水川 展吉, 小野田 友男, 江口 元治

    頭頸部癌   37 ( 2 )   220 - 220   2011.5

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  • 化学放射線療法を実施した頭頸部癌患者における、口腔粘膜炎と、C反応性タンパク、酸化ストレスの関係

    横井 彩, 山中 玲子, 丸山 貴之, 玉木 直文, 森田 学

    日本口腔ケア学会雑誌   5 ( 1 )   109 - 109   2011.1

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  • 新入生歯科健診からみえてきたこと

    江國大輔, 古田美智子, 入江浩一郎, 東 哲司, 児島 梓, 水谷慎介, 山中玲子, 友藤孝明, 森田 学, 山本龍生, 古賀 光, 岩崎良章, 清水幸登, 大西 勝, 小倉俊郎

    第41回中国・四国大学保健管理研究集会報告書   2011

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  • ウレタンジメタクリレート系レジンをマトリックスとしたフィッシャーシーラント材料の試作と物性

    江崎光恵, 入江正郎, 田仲持郎, 長岡紀幸, 森田 学

    日本歯科理工学会誌   30 ( 1 )   63 - 73   2011

  • Relationship between the prognosis of periodontitis and occlusal force during the maintenance phase - a cohort study

    N. Takeuchi, D. Ekuni, T. Yamamoto, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   45 ( 5 )   612 - 617   2010.10

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    Background and Objective:
    Few studies have longitudinally investigated the relationship between periodontal disease progression and occlusal factors in individual subjects during the maintenance phase of periodontal therapy. The aim of this cohort study was to investigate the relationship between biting ability and the progression of periodontal disease in the maintenance phase.
    Material and Methods:
    A total of 194 patients were monitored for 3 years during the maintenance phase of periodontal therapy. The subjects with disease progression (Progress group) were defined based on the presence of &gt;= 2 teeth demonstrating a longitudinal loss of proximal attachment of &gt;= 3 mm or tooth-loss experience as a result of periodontal disease during the study period. The subjects with high occlusal force were diagnosed as men who showed an occlusal force of more than 500 N and women who showed an occlusal force of more than 370 N. The association between biting ability and the progression of periodontitis was investigated using logistic regression analysis.
    Results:
    There were 83 subjects in the Progress group and 111 subjects in the Non-progress group. A backward, stepwise logistic regression model showed that the progression of periodontal disease was significantly associated with the presence of one or more teeth with a high clinical attachment level (CAL) of &gt;= 7 mm (odds ratio: 2.397; 95% confidence interval: 1.306-4.399) ( p = 0.005) and low occlusal force (odds ratio: 2.352; 95% confidence interval: 1.273-4.346) ( p = 0.006).
    Conclusion:
    The presence of one or more teeth with a high CAL of &gt;= 7 mm and low occlusal force might be possible risk factors for periodontal progression in the maintenance phase of periodontal therapy.

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  • 小学生における視力と口唇閉鎖力の関係

    山中 玲子, 古田 美智子, 江崎 光恵, 江國 大輔, 丸山 貴之, 岡崎 好秀, 森田 学

    口腔衛生学会雑誌   60 ( 5 )   606 - 606   2010.10

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  • 頭頸部がん患者における口腔内の状態と血清アルブミン値の関係

    丸山 貴之, 山中 玲子, 横井 彩, 古田 美智子, 江國 大輔, 森田 学

    口腔衛生学会雑誌   60 ( 4 )   461 - 461   2010.8

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  • Development of the Self-efficacy Scale for Maternal Oral Care

    Naoki Kakudate, Manabu Morita, Makoto Sugai, Masato Nagayama, Masamitsu Kawanami, Yuji Sakano, Itsuo Chiba

    PEDIATRIC DENTISTRY   32 ( 4 )   310 - 315   2010.7

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    Purpose: This study&apos;s purpose was to develop a task-specific self-efficacy scale for maternal oral care (SESMO) with reliability and validity. Methods: A draft scale consisting of 36 items was designed and tested on 119 mothers at a dental clinic. Item analysis was performed to improve its accuracy by reducing the number of items. Internal consistency and test and retest stability were investigated. Concurrent validity was tested by examining associations of the SESMO score with the: general self-efficacy scale (GSES) score; number of decayed teeth; and frequency of tooth-brushing for children. Construct validity was examined by comparing the SESMO scores between mothers of children with caries at initial visit (Group 1) and those continuing regular dental checkups (Group 2). Results: The item analysis selected 16 items for the SESMO. Alpha coefficient of the SESMO was 0.82. Significant correlation was observed between tests and retests (r=0.74). There were significant associations between the SESMO score and GSES score (r=0.39); number of decoyed teeth (r=-0.33); and frequency of brushing children&apos;s teeth (r=0.66). Group 2 had a significantly higher SESMO score than Group 1 (P&lt;.001). Conclusions: The SESMO showed high reliability and validity This scale might endure with research applications in pediatric dental practice. (Pediatr Dent 2010;32:310-5) Received February 13, 2009 vertical bar Accepted May 16, 2009

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  • Experimental Periodontitis Induces Gene Expression of Proinflammatory Cytokines in Liver and White Adipose Tissues in Obesity

    Yasumasa Endo, Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    JOURNAL OF PERIODONTOLOGY   81 ( 4 )   520 - 526   2010.4

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    Background: Recent studies indicated that periodontitis induces systemic low-grade inflammation. The increase in systemic low-grade inflammation induced by periodontitis may alter the effects of obesity on the production of inflammatory molecules, including C-reactive protein (CRP), interleukin (IL)-6, and tumor necrosis factor-alpha (TNF-alpha), in the liver and white adipose tissue (WAT). The purpose of the present study is to investigate the effects of periodontitis on the expression of proinflammatory cytokines in the liver and WAT in obese Zucker rats.
    Methods: Obese Zucker rats and their lean litter mates were divided into four groups of six rats each: lean Zucker rats without periodontitis (control group), lean Zucker rats with periodontitis (periodontitis group), obese Zucker rats without periodontitis (obesity group), and obese Zucker rats with periodontitis (combination group). Periodontitis was ligature induced for 4 weeks in the periodontitis and combination groups, whereas the other groups were left unligated.
    Results: At 4 weeks, the gene expression for CRP, IL-6, and TNF-alpha in the liver and CRP and IL-6 in the WAT of combination groups was significantly higher than in each of the three groups. Serum TNF-alpha in the periodontitis and obesity groups was significantly higher than in the control group. Serum CRP and TNF-alpha in the combination group was significantly higher than in each of the three groups.
    Conclusion: Systemic low-grade inflammation after experimental periodontitis was associated with increased gene expression for hepatic levels of TNF-alpha and CRP and adipose tissue levels of IL-6 and CRP in the obese-rat model. J Periodontol 2010;81:520-526.

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  • Effects of periodontitis on aortic insulin resistance in an obese rat model

    Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Tetsuji Azuma, Naofumi Tamaki, Toshihiro Sanbe, Yasumasa Endo, Tatsuo Yamamoto, Takashi Nishida, Manabu Morita

    LABORATORY INVESTIGATION   90 ( 3 )   348 - 359   2010.3

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    The combination of obesity and its associated risk factors, such as insulin resistance and inflammation, results in the development of atherosclerosis. However, the effects of periodontitis on atherosclerosis in an obese body remain unclear. The aim of the study was to investigate the effects of ligature-induced periodontitis in Zucker fatty rats on initiation of atherosclerosis by evaluating aortic insulin resistance. Zucker fatty rats (n = 24) were divided into two groups. In the periodontitis group, periodontitis was ligature-induced for 4 weeks, whereas the control group was left unligated. After the 4-week experimental period, descending aorta was used for measuring the levels of lipid deposits, immunohistochemical analysis, and evaluation of gene expression. Levels of serum C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-alpha), and insulin were also measured. Rats in the periodontitis group had significantly enhanced lipid deposits in the aorta, but not in the control group. Expression of suppressor of cytokine signaling 3, vascular cell adhesion molecule 1, reactive oxygen species, nitrotyrosine, and endothelin-1 in the periodontitis group was more intense than that in the control group. Significantly decreased levels of phosphatidylinositol 3-kinase (Pi3k) catalytic beta-polypeptide (Pi3kcb), Pi3kp85, and insulin receptor substrate 1 and 2 were observed in the periodontitis group. Levels of serum CRP and TNF-alpha were significantly increased in the periodontitis group. Under insulin-stimulated conditions, aorta in the periodontitis group altered the Akt phosphorylation. Periodontitis in obesity induced the initial stage of atherosclerosis and disturbed aortic insulin signaling.

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  • Association between self-efficacy and loss to follow-up in long-term periodontal treatment. International journal

    Naoki Kakudate, Manabu Morita, Shin Yamazaki, Shunichi Fukuhara, Makoto Sugai, Masato Nagayama, Masamitsu Kawanami, Itsuo Chiba

    Journal of clinical periodontology   37 ( 3 )   276 - 82   2010.3

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    Aim: To examine whether or not oral health care-specific self-efficacy can predict loss to follow-up for long-term periodontal treatment.
    Methods: Our prospective cohort study enrolled 144 patients with chronic periodontitis. Patient self-efficacy was assessed on the initial visit using the self-efficacy scale for self-care (SESS). Participants were then followed for 30 months from the onset of periodontal treatment. The loss to follow-up is the main outcome variable. Multiple logistic regression analysis was conducted to examine the association between self-efficacy for self-care and loss to follow-up for periodontal treatment. Gender, age, number of teeth, probing depth, plaque control record (PCR) value, PCR improvement rate, general self-efficacy scale score, and SESS score were used as the independent variables, and loss to follow-up as a dependent variable.
    Results: A total of 67 patients were lost to follow-up over the course of the study. Compared with the high-scoring SESS group (60-75), the odds ratio of loss to follow-up for the middle- (54-59) and low-scoring groups (15-53) were 1.05 [95% confidence interval (CI): 0.36-3.07] and 4.56 (95% CI: 1.11-18.74), respectively.
    Conclusion: Assessment of oral health care-specific self-efficacy may be useful in predicting loss to follow-up in long-term periodontal treatment.

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  • 周術期における歯科介入の重要性 岡山大学病院周術期管理センター歯科部門の取り組み

    丸山 貴之, 山中 玲子, 曽我 賢彦, 縄稚 久美子, 柳 文修, 兒玉 直紀, 中田 貴, 村田 尚道, 有岡 享子, 山根 美榮子, 三浦 留美, 羽川 操, 木南 美香, 高橋 明子, 住吉 由季子, 竹内 哲男, 窪木 拓男, 佐々木 朗, 森田 学

    日本口腔ケア学会雑誌   4 ( 1 )   83 - 83   2010.3

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  • Imbalance of oxidative/anti-oxidative status induced by periodontitis is involved in apoptosis of rat submandibular glands

    Daisuke Ekuni, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takaaki Tomofuji, Manabu Morita

    ARCHIVES OF ORAL BIOLOGY   55 ( 2 )   170 - 176   2010.2

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    Objective: Epidemiologic studies suggest a relationship between periodontitis and salivary gland dysfunction. A rat periodontitis model was used to investigate whether a causal relationship exists between periodontitis and pathological changes of submandibular glands.
    Design: Fourteen male Wistar rats (8 weeks old) were divided into two groups (n = 7/group): a control group and periodontitis group. Periodontitis was induced by ligature placement around the mandibular first molars. Serum levels for reactive oxygen metabolites, antioxidant and tumour necrosis factor (TNF)-alpha were measured at baseline, 2 and 4 weeks. At 4 weeks, the levels of 8-hydroxydeoxyguanosine were determined to evaluate oxidative damage of submandibular glands. Expression of TNF-alpha mRNA and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) as well as histological findings were also evaluated in the submandibular glands.
    Results: The rats with experimental periodontitis showed increase in the levels of serum reactive oxygen metabolites and TNF-alpha, and a decrease of anti-oxidant power in a time-dependent manner. At 4 weeks, these rats also had significantly increased levels of 8-hydroxydeoxyguanosine and TNF-alpha, and increased number of TUNEL-positive cells and vacuolisation in the submandibular glands compared to the control rats.
    Conclusions: Imbalance of circulating oxidative/anti-oxidative status may be involved in vacuolisation and apoptosis of submandibular glands in the rat periodontitis model. (C) 2009 Elsevier Ltd. All rights reserved.

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  • Comparison of dental practice income and expenses according to treatment types in the Japanese insurance system

    Naoki Kakudate, Manabu Morita, Makoto Sugai, Masato Nagayama, Shunichi Fukuhara, Masamitsu Kawanami, Itsuo Chiba

    Japanese Dental Science Review   46 ( 1 )   4 - 10   2010.2

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    Dental practice income and expenses according to treatment types were compared in order to obtain information for cost accounting of dental treatments and to establish successful management methods for dental treatments in order to improve the balance of dental clinics. The results of our study are as follows:(1)For class I restoration for molars, the balance of inlay restoration was higher than that of CR filling, while the balance per minute for CR filling was higher than that for inlay restoration. Inlay restoration would be more profitable for dental clinics with a relatively small number of patients, whereas for clinics with many patients, composite resin filling would be more profitable to increase income.(2)There is a need for a system to increase the number of patients receiving regular dental check-ups.(3)It is important to train dental hygienists to perform regular dental check-ups.(4)There is a need to reconsider the present medical fee points for oral hygiene instruction and root canal treatment because their balance per minute is quite low.(5)It is important to establish a system to increase the number of patients who choose non-insured treatment for crown restorations in order to increase the management balance of dental clinics in Japan. © 2009 Japanese Association for Dental Science.

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  • Comparison of dental practice income and expenses according to treatment types in the Japanese insurance system

    Naoki Kakudate, Manabu Morita, Makoto Sugai, Masato Nagayama, Shunichi Fukuhara, Masamitsu Kawanami, Itsuo Chiba

    Japanese Dental Science Review   46 ( 1 )   4 - 10   2010.2

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    Dental practice income and expenses according to treatment types were compared in order to obtain information for cost accounting of dental treatments and to establish successful management methods for dental treatments in order to improve the balance of dental clinics. The results of our study are as follows:(1)For class I restoration for molars, the balance of inlay restoration was higher than that of CR filling, while the balance per minute for CR filling was higher than that for inlay restoration. Inlay restoration would be more profitable for dental clinics with a relatively small number of patients, whereas for clinics with many patients, composite resin filling would be more profitable to increase income.(2)There is a need for a system to increase the number of patients receiving regular dental check-ups.(3)It is important to train dental hygienists to perform regular dental check-ups.(4)There is a need to reconsider the present medical fee points for oral hygiene instruction and root canal treatment because their balance per minute is quite low.(5)It is important to establish a system to increase the number of patients who choose non-insured treatment for crown restorations in order to increase the management balance of dental clinics in Japan. © 2009 Japanese Association for Dental Science.

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  • マルチレベル分析による親の喫煙行動と社会環境に関する研究

    竹内研時, 相田潤, 森田学, 安藤雄一, 小坂健

    J Epidemiol   20 ( Supplement 1 )   S117   2010.1

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  • Relationship between periodontitis and hepatic abnormalities in young adults. International journal

    Michiko Furuta, Daisuke Ekuni, Tatsuo Yamamoto, Koichiro Irie, Reiko Koyama, Toshihiro Sanbe, Reiko Yamanaka, Manabu Morita, Kiyomi Kuroki, Kazuo Tobe

    Acta odontologica Scandinavica   68 ( 1 )   27 - 33   2010.1

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    Objective. Obesity has been implicated as a risk factor for periodontitis and non-alcoholic fatty liver disease (NAFLD). In NAFLD, elevated alanine aminotransferase (ALT) is associated with obesity. Although a possible interrelationship between liver function and periodontitis has been reported among the middle-aged population, the correlation in young adults is little known. This study was designed to investigate the relationship between ALT and the presence of periodontitis in university students in Japan. Material and methods. Medical and oral health data were collected in a cross-sectional examination conducted by the Health Service Center of Okayama University. Systemically healthy, non-smoking students aged 18 and 19 years old (n = 2225) were included. The protocol of the United States National Health and Nutrition Examination Survey was applied. Subjects with probing pocket depth &gt;= 4 mm were defined as having periodontitis. Logistic regression analysis was used to estimate the association between ALT, body mass index and periodontitis. Results. The number of subjects with periodontitis was 104 (4.7%,). In males, having periodontitis was significantly associated with an increased level of ALT ( ! 41 IU/l) in logistic regression analysis (adjusted odds ratio 2.3; 95% confidence interval 1.0-5.2; p &lt; 0.05). However, there was no significant association between periodontitis and ALT in female students. Conclusions. Elevated ALT could be a potential risk indicator for periodontitis among young males. Monitoring hepatic abnormalities to prevent periodontitis must be better understood, even in the young adult population.

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  • Relationship between folic acid intake and gingival health in non-smoking adults in Japan

    M. Esaki, M. Morita, R. Akhter, K. Akino, O. Honda

    ORAL DISEASES   16 ( 1 )   96 - 101   2010.1

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    Objective:
    To assess the relationship between dietary intake of folate and gingival bleeding in non-smoking adults in Japan.
    Materials And Methods:
    Data were obtained from residents who participated in the regional nutrition survey and survey of dental diseases conducted by the administrative office of northernmost prefecture of Japan. Dietitians visited households to collect data on dietary intake. Clinical parameters, including Community Periodontal Index (CPI) and bleeding on probing (BOP), were examined in community centers. Information on smoking habit was obtained from the interview. Then the data from 497 non-smoking adults with 20 teeth or more, aged 18 years or older, were analyzed. The relationship between dietary intake of folic acid and gingival bleeding status was estimated using multivariate analysis.
    Results:
    Pearson&apos;s correlation coefficient showed a significant negative correlation between dietary folate level and bleeding on probing. The negative association between folate level and bleeding on probing remained statistically significant in multiple regression analysis (standardized beta = -0.204, P &lt; 0.001). However, no significant association was found between CPI scores and folate intake level.
    Conclusions:
    The results suggest that dietary intake of folic acid, an important indicator of gingival bleeding in adults, may provide an important clinical target for intervention to promote gingival health.

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  • 地域の歯科医療関係者に対する支援 –口腔インプラント講習会と摂食・嚥下リハビリテーション教育–

    東 哲司, 有岡享子, 荒川 光, 江草正彦, 窪木拓男, 森田 学

    岡山医学会雑誌   121 ( 3 )   183 - 188   2010

  • 女子中学生における口やのどの乾燥に関連した自覚症状に共通する要因

    兼平 孝, 竹原順次, 中村公也, 三宅 亮, 本多丘人, 森田 学

    日本口腔衛生学会雑誌   60 ( 3 )   233 - 238   2010

  • メインテナンス期の歯周病患者における咬合力と歯周病進行との関係について

    竹内倫子, 江國大輔, 山本龍生, 森田 学

    岡山歯学会雑誌   29 ( 1 )   41 - 50   2010

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  • 卒前歯学臨床教育におけるインターンシップ実習の構築と今後の展望

    前川賢治

    岡山歯学会雑誌   29 ( 1 )   35 - 40   2010

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  • Relatioship between perdiodontal status and components of metabolic syndrome in a rural Japanese population.

    Yamamotot T, Tsuneishi M, Furuta M, Koyama R, Ekuni D, Morita M, Hirata Y

    日本口腔衛生学会雑誌   60 ( 2 )   96 - 103   2010

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  • ワークショップ「頭頸部がんチーム医療における口腔ケアの促進」と連携強化.

    山中玲子, 丸山貴之, 横井 彩, 山田小百合, 玉木直文, 江國大輔, 友藤孝明, 森田 学

    岡山歯学会雑誌   61   2010

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  • Preventive Effects of a Cocoa-Enriched Diet on Gingival Oxidative Stress in Experimental Periodontitis

    Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Yasumasa Endo, Naofumi Tamaki, Toshihiro Sanbe, Jun Murakami, Tatsuo Yamamoto, Manabu Morita

    JOURNAL OF PERIODONTOLOGY   80 ( 11 )   1799 - 1808   2009.11

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    Background: Oxidative stress affects the progression of periodontitis. Cocoa is a rich source of flavonoids with antioxidant properties, which could suppress gingival oxidative stress in periodontal lesions. The purpose of the present study was to investigate the effects of a cocoa-enriched diet on gingival oxidative stress in a rat-periodontitis model.
    Methods: In this 4-week study, rats were divided into three groups (n = 8/group): a control group (fed a regular diet) and two periodontitis groups (fed a regular diet or cocoa-enriched diet [10% of food intake]). Periodontitis was induced by ligature placement around the mandibular first molars. Serum levels for reactive oxygen metabolites were measured at baseline and 2 and 4 weeks. At 4 weeks, the levels of 8-hydroxydeoxyguanosine and reduced/oxidized glutathione ratio were determined to evaluate gingival oxidative damage and antioxidant status, respectively.
    Results: Rats with experimental periodontitis that were fed a regular diet showed an increase in the level of serum reactive oxygen metabolites in a time-dependent manner. These rats also had an increased 8-hydroxydeoxyguanosine level and decreased reduced/oxidized glutathione ratio in the gingival tissue, inducing alveolar bone loss and polymorphonuclear leukocyte infiltration. Although experimental periodontitis was induced in the rats fed a cocoa-enriched diet, they did not show impairments in serum reactive oxygen metabolite level and gingival levels for 8-hydroxydeoxyguanosine and reduced/oxidized glutathione ratio. Alveolar bone loss and polymorphonuclear leukocyte infiltration after ligature placement were also inhibited by cocoa intake.
    Conclusion: Consuming a cocoa-enriched diet could diminish periodontitis-induced oxidative stress, which, in turn, might suppress the progression of periodontitis. J Periodontol 2009;80:1799-1808.

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  • 急性期病院における頭頸部癌患者への口腔ケア

    山中 玲子, 小山 玲子, 江國 大輔, 古田 美智子, 丸山 貴之, 山本 龍生, 森田 学

    口腔衛生学会雑誌   59 ( 5 )   609 - 610   2009.10

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  • A single application of hydrogen sulphide induces a transient osteoclast differentiation with RANKL expression in the rat model. International journal

    Koichiro Irie, Daisuke Ekuni, Tatsuo Yamamoto, Manabu Morita, Ken Yaegaki, Hisataka Ii, Toshio Imai

    Archives of oral biology   54 ( 8 )   723 - 9   2009.8

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    Objective: Oral malodor is mainly attributed to volatile sulphur compounds (VSCs) such as hydrogen sulphide (H2S), methyl mercaptan and dimethyl sulphide. VSC accelerate periodontal soft tissue destruction. However, there is little information about the potential role of H2S in alveolar bone loss. The purpose of this animal study was to examine the effects of sodium hydrogen sulphide (NaHS), H2S donor drug, on osteoclast differentiation in rat periodontal tissue.
    Design: Twenty-four male Wistar rats (8 weeks old) were divided into four groups: a control group and three experimental groups, which were examined at 3 h, 1 day, and 3 days after topical application of 3 mu l NaHS (l M in physiological saline) into the gingival sulcus of rat first molar. Expression of tumour necrosis factor (TNF)-alpha, RANKL, NF-kappa B and tartrate-resistant acid phosphatase (TRAP) was evaluated in the periodontal tissue.
    Results: Three hours after NaHS application, TNF-alpha expression increased in the periodontal ligament. The numbers of RANKL-positive osteoblasts and TRAP-positive osteoclasts significantly increased progressively with time and reached a maximum level after 1 day. Significant up-regulation of RANKL and NF-kappa B mRNA was observed at 3 h after NaHS application.
    Conclusions: H2S application caused a transient increase of osteoclast differentiation with up-regulation of RANKL expression in osteoblasts. H2S, which is primarily responsible for halitosis, may also contribute to alveolar bone resorption through RANKL expression. (C) 2009 Elsevier Ltd. All rights reserved.

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  • Effects of obesity on gingival oxidative stress in a rat model. International journal

    Takaaki Tomofuji, Tatsuo Yamamoto, Naofumi Tamaki, Daisuke Ekuni, Tetsuji Azuma, Toshihiro Sanbe, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Jun Murakami, Susumu Kokeguchi, Manabu Morita

    Journal of periodontology   80 ( 8 )   1324 - 9   2009.8

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    Background: Studies indicate a correlation between obesity and periodontitis. Oxidative stress is involved in the progression of periodontitis. The purpose of this study was to investigate the effects of obesity on gingival oxidative stress in a rat periodontitis model.
    Methods: The obese Zucker rats (n = 14) and their lean littermates (n = 14) were each divided into two groups of seven rats. In one of each group, periodontitis was induced by ligature for 4 weeks, whereas the other group was left unligated. The level of 8-hydroxydeoxyguanosine and the ratio of reduced/oxidized glutathione were determined to examine gingival oxidative stress. The serum level of reactive oxygen metabolites and the gingival gene-expression pattern related to oxidative/metabolic stress, inflammation, and cell behavior were also evaluated.
    Results: The obese rats weighed more than the lean rats at 4 weeks. Compared to lean rats, obese rats had enhanced gingival 8-hydroxydeoxyguanosine levels and a decreased ratio of reduced/oxidized glutathione in the gingival tissue, with increasing serum reactive oxygen metabolites. However, there were no significant differences in the degree of alveolar bone loss between lean and obese rats, except for teeth with and without ligatures in both rats. In addition, the periodontal lesion in obese rats showed higher 8-hydroxydeoxyguanosine levels and polymorphonuclear leukocyte infiltration than the inflamed ones in lean rats, with downregulation of multiple cytochrome P450 gene expression.
    Conclusions: Obesity induced gingival oxidative stress with increasing serum reactive oxygen metabolites in rats. In the periodontal lesion, gene expressions related to a capacity for xenobiotic detoxification were downregulated in the obese model. J Periodontol 2009;80:1324-1329.

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  • 食器の共用や食物の口移しを注意すれば、う蝕は予防できるのか?

    若栗 真太郎, 相田 潤, 森田 学, 安藤 雄一, 小坂 健

    口腔衛生学会雑誌   59 ( 4 )   313 - 313   2009.8

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  • Short-term effects of non-surgical periodontal treatment on plasma level of reactive oxygen metabolites in patients with chronic periodontitis. International journal

    Naofumi Tamaki, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Tatsuo Yamamoto, Manabu Morita

    Journal of periodontology   80 ( 6 )   901 - 6   2009.6

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    Background: Elevated levels of blood reactive oxygen species (ROS) are associated with the severity of periodontitis. Therefore, improvement of periodontitis may result in a decrease in blood ROS. However, it is unclear how periodontal treatment affects blood ROS. Recently, reactive oxygen metabolites (ROMs) were recognized as a useful measure of blood ROS. The aim of this longitudinal study was to investigate the effect of non-surgical periodontal treatment on plasma ROMs in patients with chronic periodontitis.
    Methods: Nineteen subjects with chronic periodontitis (mean age: 46.8 years) were monitored at baseline (prior to scaling and root planing) and I and 2 months after therapy. Dental health parameters were evaluated, and plasma was obtained at these time points from patients and controls (19 subjects without periodontitis; mean age: 45.3 years), The plasma ROM level was determined using a spectrophotometric technique.
    Results: At baseline, patients with chronic periodontitis had higher plasma ROM level (441.8 +/- 71.1 Carratelli units) than the control subjects (324.4 +/- 34.0 Carratelli units; P &lt; 0.01). Probing depth, clinical attachment level, and bleeding on probing in patients with chronic periodontitis showed a significant improvement 2 months after non-surgical periodontal treatment, and this was accompanied by a significant reduction in plasma ROM level (P &lt; 0.01).
    Conclusions: In patients with chronic periodontitis, nonsurgical periodontal treatment was effective at improving clinical parameters and reducing plasma ROMs. The improvement in chronic periodontitis by non-surgical periodontal treatment might offer clinical benefits by decreasing blood ROS. J Periodontol 2009;80:907-906.

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  • Relationships between patient characteristics and reasons for tooth extraction in Japan

    J. Aida, M. Morita, R. Akhter, H. Aoyama, M. Masui, Y. Ando

    COMMUNITY DENTAL HEALTH   26 ( 2 )   104 - 109   2009.6

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    Objectives: The purpose of this study was to examine the relationships between patient characteristics and reasons for extraction of permanent teeth. Methods: 5,131 dentists were selected from the list of the membership directory of the Japan Dental Association by systematic random selection. The dentists were asked to record the reason for each extraction of permanent teeth during a period from February I to 7, 2005. Reasons for tooth extraction were assigned to five groups: caries, fracture of teeth weakened by caries or endodontics, periodontal diseases, orthodontics and other reasons. We used cross tabulation and multiple logistic regression analysis to estimate the relationships between patient characteristics and reasons for tooth extraction. Results: 2,001 dentists (response rate of 39.0%) returned the forms, and complete information on 7,333 patients was obtained. A total of 3,196 (43.6%) patients underwent tooth extraction due to caries and its sequela, and 2,721 (37.1%) patients underwent tooth extraction due to periodontal disease. Multiple logistic regression analysis showed that denture wearers were more likely to undergo tooth extraction due to periodontal disease in all age groups (p&lt;0.05). Males tended to undergo tooth extraction due to periodontal disease than did females in all age groups (p&lt;0.05) except for age group 3049. Subjects with 19 or less teeth were more likely to undergo tooth extraction due to periodontal disease in the age groups 30-49 (p&lt;0.001) and 50-69 (p&lt;0.001). In the age group of 50 years or older, female (p&lt;0.01) and the possession of 20 or more natural teeth (p&lt;0.05) were related to caries extraction. However, there was no clear relationship between caries extraction and patient characteristics under 50 years old. Conclusion: There was a significant relationship between denture wearing and periodontal extraction. In the middle aged population, patients with 19 or less teeth lost their teeth mainly due to periodontal disease.

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  • アンケートによる歯周疾患スクリーニングの有効性

    小山 玲子, 玉木 直文, 丸山 貴之, 友藤 孝明, 江國 大輔, 山中 玲子, 東 哲司, 山本 龍生, 森田 学

    岡山歯学会雑誌   28 ( 1 )   88 - 88   2009.6

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  • Periodontitis and increase in circulating oxidative stress

    Takaaki Tomofuji, Koichiro Irie, Toshihiro Sanbe, Tetsuji Azuma, Daisuke Ekuni, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    Japanese Dental Science Review   45 ( 1 )   46 - 51   2009.5

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    Reactive oxygen species (ROS) are products of normal cellular metabolism. However, excessive production of ROS oxidizes DNA, lipids and proteins, inducing tissue damage. Studies have shown that periodontitis induces excessive ROS production in periodontal tissue. When periodontitis develops, ROS produced in the periodontal lesion diffuse into the blood stream, resulting in the oxidation of blood molecules (circulating oxidative stress). Such oxidation may be detrimental to systemic health. For instance, previous animal studies suggested that experimental periodontitis induces oxidative damage of the liver and descending aorta by increasing circulating oxidative stress. In addition, it has been revealed that clinical parameters in chronic periodontitis patients showed a significant improvement 2 months after periodontal treatment, which was accompanied by a significant reduction of reactive oxygen metabolites in plasma. Improvement of periodontitis by periodontal treatment could reduce the occurrence of circulating oxidative stress. Furthermore, recent studies indicate that the increase in circulating oxidative stress following diabetes mellitus and inappropriate nutrition damages periodontal tissues. In such cases, therapeutic approaches to systemic oxidative stress might be necessary to improve periodontal health. © 2009 Japanese Association for Dental Science.

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  • Vitamin C intake inhibits serum lipid peroxidation and osteoclast differentiation on alveolar bone in rats fed on a high-cholesterol diet. International journal

    Toshihiro Sanbe, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    Archives of oral biology   54 ( 3 )   235 - 40   2009.3

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    Objective: A high-cholesterol diet stimulates osteoclast differentiation, which may be induced by increased serum lipid peroxidation. The inhibition of serum lipid peroxidation by vitamin C may offer beneficial effects on osteoclast differentiation including increased expression of receptor activator of nuclear factor (NF)-kappa B ligand (RANKL) and NF-kappa B. This study investigated the effects of vitamin C intake on RANKL and NF-kappa B expression in periodontal tissue of rats fed a high-cholesterol diet.
    Design: Twenty-four rats (8 weeks old) were divided into four groups: a control group (fed a regular diet) and three experimental groups (fed a high-cholesterol diet supplemented with 0,1 and 2 g/I vitamin C/day) in this 12-week study. Vitamin C was provided by its addition to drinking water. As an index of serum lipid peroxidation, hexanoyl-lysine (HEL) level was determined by a competitive enzyme-linked immunosorbent assay method. Immunohistological analysis was performed to evaluate RANKL and NF-kappa B expression on the alveolar bone surface. The number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts was also counted.
    Results: Feeding a high-cholesterol diet increased not only the serum HEL level but also the number of TRAP-positive osteoclasts on the alveolar bone surface, with an increase in RANKL and NF-kappa B expression on alveolar bone surface. intake of vitamin C reduced the serum HEL level and osteoclast differentiation, with decreasing RANKL and NF-kappa B expression.
    Conclusions: Vitamin C intake could suppress osteoclast differentiation, including RANKL and NF-kappa B expression on the alveolar bone surface, by decreasing serum lipid peroxidation in rats fed a high-cholesterol diet. (c) 2008 Elsevier Ltd. All rights reserved.

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  • Systematic cognitive behavioral approach for oral hygiene instruction: a short-term study. International journal

    Naoki Kakudate, Manabu Morita, Makoto Sugai, Masamitsu Kawanami

    Patient education and counseling   74 ( 2 )   191 - 6   2009.2

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    Objective: Determine whether a six-step behavioral cognitive method is more effective than traditional oral hygiene instruction.
    Methods: Thirty-eight patients with chronic periodontitis were randomly assigned to two groups. The control group was given traditional oral hygiene instruction for 20 min. The intervention group received counseling by Farquhar&apos;s six-step method for 10 min after traditional oral hygiene instruction. In both groups, oral hygiene instruction was given once a week, and performed three times in total for 3 weeks. Clinical characteristics, deposition of dental plaque, frequency and duration of brushing, frequency of interdental cleaning and scores based on scale of "self-efficacy for brushing of the teeth" were compared in both groups.
    Results: There were no differences between the two groups in clinical, demographic, behavioral and self-efficacy characteristics at the baseline examination. However after the third visit, the intervention group had a significantly higher self-efficacy, lower plaque index, longer brushing duration and higher frequency of inter-dental cleaning than those of the control group. Multiple regression analysis showed significant association of toothbrushing duration with self-efficacy for brushing of the teeth (p &lt; 0.001).
    Conclusion: The six-step method might be more effective for enhancing self-efficacy and behavioral change of oral hygiene than traditional oral hygiene instruction alone.
    Practice implications: Dentists and dental hygienists Call use the six-step method for effective oral hygiene instruction. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

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  • 歯周疾患対策として液体歯磨剤,抗炎症薬およびビタミン類を配合する意義 –ラットの実験的歯周炎を用いての組織学的・免疫組織化学的検討-

    入江浩一郎

    日本歯周病学会雑誌   51 ( 3 )   252 - 259   2009

  • Validity of a questionnaire for periodontitis screening of Japanese employees. International journal

    Tatsuo Yamamoto, Reiko Koyama, Naofumi Tamaki, Takayuki Maruyama, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Tetsuji Azuma, Manabu Morita

    Journal of occupational health   51 ( 2 )   137 - 43   2009

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    OBJECTIVES: Workplaces are suitable for screening for periodontal disease effectively. However, the majority of workplaces do not have a screening program. One possible reason may be that pocket probing, which is commonly used for the screening, needs qualified examiners and is time-consuming. In this study, the validity of a self-reported questionnaire was assessed for screening of periodontitis in 50- to 59-yr-old male employees in Japan. METHODS: A total of 250 people were asked to complete a self-administered questionnaire regarding signs and symptoms of periodontal disease, and experience of periodontal treatment, followed by a clinical examination by a dentist. Subjects with at least one tooth having a clinical attachment loss of 7 mm or more were defined as periodontitis patients. RESULTS: Thirteen percent of the subjects were diagnosed as having periodontitis. Logistic regression selected 4 questions, "Are you a current or past smoker?", "Have your gums bled recently?", "Do you think that you can see more roots of teeth than in the past?", and "Have you ever been told that you need periodontal or gum treatment?" as potential predictors of periodontitis. When subjects with at least 3 "yes" responses to the 4 questions were separated from the others, the subjects with periodontitis were separated most effectively (showing the highest sensitivity + specificity: 1.524) from those without. The area under the receiver operating characteristic curve was 0.81. CONCLUSIONS: These results suggest that the self-reported questions are useful for screening of periodontitis in 50- to 59-yr-old Japanese workers.

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  • Health Oriented Dentistryと“つまようじ法”

    森田 学

    歯科医療   23 ( 4 )   3 - 40   2009

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  • お口の健康を通じての快適生活・快適職場

    森田 学

    ベネッセグルー健保だより   4 - 5   2009

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  • マウスガードの身体能力に及ぼす影響について

    谷健作, 玉木直文, 森田 学

    岡山歯学会雑誌   28 ( 2 )   115 - 121   2009

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  • Simple screening for dry mouth.

    Kanehira T, Yamaguchi T, Takehara J, Kashiwazaki H, Abe T, Morita M, Asano K, Fujii Y, Sakamoto W

    Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endododontology   108 ( 3 )   199 - 207   2009

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  • 岡山大学病院周術期管理センター(歯科部門)設立後5ヶ月間の活動内容および今後の展開

    山中玲子

    岡山歯学雑誌   28 ( 1 )   37 - 42   2009

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  • Biodistribution imaging of magnetic particles in mice: X-ray scanning analytical microscopy and magnetic resonance imaging. International journal

    Shigeaki Abe, Ikuhiro Kida, Mitsue Esaki, Tsukasa Akasaka, Motohiro Uo, Toshiaki Hosono, Yoshinori Sato, Balachandran Jeyadevan, Yoshinori Kuboki, Manabu Morita, Kazuyuki Tohji, Fumio Watari

    Bio-medical materials and engineering   19 ( 2-3 )   213 - 20   2009

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    Nano-sized particles have received much attention in view of their varied application in a wide range of fields. For example, magnetite (Fe(3)O(4)) nanoparticles have been investigated for various medical applications. In this study, we visualized the distribution of administered magnetic nanoparticles in mice using both X-ray scanning analytical microscopy (XSAM) and magnetic resonance imaging (MRI). After administration, the nanoparticles were rapidly dispersed via the blood circulation, and reached the liver, kidney and spleen. Using the XSAM and MRI methods in a complementary fashion, the biodistribution of nano-sized magnetite particles was successfully visualized.

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  • In vivo internal diffusion of several inorganic microparticles through oral administration. International journal

    Shigeaki Abe, Chila Koyama, Mitsue Esaki, Tsukasa Akasaka, Motohiro Uo, Yoshinori Kuboki, Manabu Morita, Fumio Watari

    Bio-medical materials and engineering   19 ( 2-3 )   221 - 9   2009

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    We observed the internal diffusion behavior of inorganic micro/nano particles through oral administration. By oral exposure, the fed particles were absorbed through the digestive system then reached some organs after internal diffusion in the body. For example, TiO(2) particles fed to mice were detected in the lung, liver, and spleen after 10 days of feeding. Whereas, the absorption efficiency was extremely low compared with intravenous injection. In a comparison of the simple amount of administration, oral exposure required 10(2) times or more amount by intravenous injection for detection by an X-ray scanning analytical microscope. During dental treatment, micro/nano particles from tooth or dental materials would generate in the oral cavity, and some of the particles had a possibility to be swallowed, absorbed through the digestive system, and then diffuse into the body. However, our results suggest that biocompatible microparticles that are naturally taken orally affect animals only rarely because of the low absorption efficiency.

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  • 大学生の口腔健康状態の調査および歯周健康状態と関連要因の検討

    古田美智子, 江國大輔, 入江浩一郎, 小山玲子, 三部俊博, 山中玲子, アクターラヘナ, 山本龍生, 馬越通弘, 粕山健太, 森田 学

    口腔衛生学会雑誌   59 ( 3 )   165 - 172   2009

  • 14歳女児にみられた上顎第一,第二大臼歯埋伏の1例-学校歯科健診の観点から-

    原田祥二, 藤田真理, 本多丘人, 森田 学

    口腔衛生学会雑誌   59 ( 3 )   165 - 172   2009

  • 歯科疾患の地域差の検討

    相田 潤, 森田 学, 安藤雄一, 丹後俊郎, 高橋邦彦, 青山 旬, 小坂 健

    保健医療科学   57 ( 2 )   93 - 98   2009

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  • Relationship between periodontal status and physical fitness in an elderly population of nonsmokers in Bangladesh. International journal

    Rahena Akhter, Nur Mohammad Monsur Hassan, Shingo Moriya, Haruhiko Kashiwazaki, Nobuo Inoue, Manabu Morita

    Journal of the American Geriatrics Society   56 ( 12 )   2368 - 70   2008.12

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    DOI: 10.1111/j.1532-5415.2008.02036.x

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  • The relationship between jaw injury, third molar removal, and orthodontic treatment and TMD symptoms in university students in Japan. International journal

    Rahena Akhter, Nur Mohammad Monsur Hassan, Ruka Ohkubo, Tetsurou Tsukazaki, Jun Aida, Manabu Morita

    Journal of orofacial pain   22 ( 1 )   50 - 6   2008.12

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    Aims: To determine the association between temporomandibular disorders (TMD) and experiences of jaw injury, third molar removal, and orthodontic treatment, controlling for confounding factors such as age, sex, emotional stress, and oral parafunction. Methods: First-year university students (n = 2,374) were instructed to answer a questionnaire regarding symptoms of TMD, jaw injury, third molar removal, orthodontic treatment, stress, and parafunctional habits. All subjects were classified according to the level of TMD symptoms. Logistic regression was applied to assess the associations of experiences of jaw injury, third molar removal, and orthodontic treatment with presence of TMD symptoms after controlling for age, sex, stress, and parafunctional habits. Results: Of the 2,374 students, 715 students were TMD symptom-positive. They were classified into 7 groups consisting of those with only clicking (group 1), only pain in the temporomandibular joint (group 2), only difficulty in mouth opening (group 3), clicking and pain (group 4), clicking and difficulty in mouth opening (group 5), difficulty in mouth opening and pain (group 6), and all 3 symptoms (group 7). TMD symptoms were significantly associated with jaw injury. Odds ratios were 2.25, 2.47, 3.38, and 2.01 for groups 2, 3, 6, and 7, respectively. Experience of third molar removal was significantly associated with TMD (odds ratio = 1.81 for group 1). No association was found between orthodontic experience and TMD. Conclusion: Experiences of jaw injury and third molar removal might be cumulative and precipitating events in TMD.

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  • Synthetic double-stranded RNA induces retinoic acid-inducible gene-I in mouse osteoblastic cells

    Kimiya Nakamura, Yoshiaki Deyama, Yoshitaka Yoshimura, Kuniaki Suzuki, Manabu Morita

    MOLECULAR MEDICINE REPORTS   1 ( 6 )   833 - 836   2008.11

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    Retinoic acid inducible gene-I (RIG-I) is a member of the DExH box family of proteins. RIG-I acts as a sensor of viral infections through the recognition of viral double-stranded RNA (dsRNA). Recently, it was demonstrated that polyinosinic acid: polycytidylic acid [poly(I):poly(C)], a synthetic dsRNA analogue, induced the expression of RIG-I in various cell types, such as vascular endothelial cells and gingival fibroblasts. However, it remains unclear whether RIG-I is induced in osteoblasts in response to poly(I): poly(C). In the present study, we investigated the effects of poly(I):poly(C) on the expression of RIG-I in mouse osteoblastic MC3T3-E1 (E1) cells. We found that poly(I):poly(C) increased the expression level of RIG-I in E1 cells, and that recombinant interferon-ss (IFN-ss) induced the expression of RIG-I mRNA in E1 cells. An anti-IFN-ss neutralizing antibody partially inhibited poly(I):poly(C)-induced RIG-I expression. These results indicate that RIG-I production is induced by poly(I):poly(C)-provoked IFN-ss in mouse osteoblastic E1 cells. We suggest that osteoblasts are involved in antiviral defense as well as in bone metabolism. Results of further studies will provide more clues regarding the molecular function of osteoblasts in viral infection.

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  • Correlations of noncarious cervical lesions and occlusal factors determined by using pressure-detecting sheet

    Junji Takehara, Tomotsugu Takano, Rahena Akhter, Manabu Morita

    JOURNAL OF DENTISTRY   36 ( 10 )   774 - 779   2008.10

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    Objectives: The purpose of this clinical study was to examine the relationships of v-shaped noncarious cervical lesion (NCCL) formation with occlusal factors.
    Methods: A total of 159 male self-defense force officials with a mean age of 315,2 years participated in this study. All present teeth were examined for the presence and type of NCCL using the Tooth Wear Index (TWI). The subjects were then interviewed about bruxing and toothbrushing habit. Finally, occlusal force, occlusal contact area and average pressure were measured using a pressure-detecting sheet. Subject-level logistic regression was carried out to assess the associations of factors with presence of v-shaped NCCL teeth. Subjects without v-shaped NCCL were designated as control subjects.
    Results: Totally, 4518 teeth were examined. Seventy-eight subjects (49.1%) had one or more teeth with typical v-shaped NCCL (259 teeth). The number of teeth with v-shaped NCCL of grade 2 (defect less than 1 mm in depth) was 195 (4.3%), and the number of teeth with v-shaped NCCL of grade 3 (defect 1-2 mm in depth) was 54 (1.2%). The prevalence of teeth with v-shaped NCCL was significantly higher in the maxilla than in the mandible. Most of the NCCL teeth were premolars. There was no significant difference between teeth with NCCL on the right side and those on the left side. Subject-level logistic regression analysis revealed that age (OR = 1.11), toothbrushing pressure (400 g, OR = 2.43) and occlusal contact area (&gt;23.0 MM2, OR = 4.15) were associated with the presence of NCCL teeth.
    Conclusions: it is concluded that aging, toothbrushing pressure and occlusal contact area are associated with the presence of NCCLs. (c) 2008 Elsevier Ltd. All rights reserved.

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  • 歯周炎モデルラットにおける歯肉酸化ストレス度・抗酸化力に対するビタミンC全身投与の効果

    入江 浩一郎, 友藤 孝明, 江國 大輔, 三部 俊博, 東 哲司, 丸山 貴之, 玉木 直文, 山本 龍生, 森田 学

    口腔衛生学会雑誌   58 ( 4 )   454 - 454   2008.8

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  • Oral health care-specific self-efficacy assessment predicts patient completion of periodontal treatment: a pilot cohort study. International journal

    Naoki Kakudate, Manabu Morita, Masamitsu Kawanami

    Journal of periodontology   79 ( 6 )   1041 - 7   2008.6

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    Background: Whether patients complete periodontal treatment is an important matter of concern. Self-efficacy is the confidence of an individual that determines "how well he/she can take the actions necessary for producing certain results" and is a variable that forecasts behavior. This study examined whether oral health care-specific self-efficacy can predict patient completion of periodontal treatment.
    Methods: A total of 140 subjects with mild to moderate chronic periodontitis (64 females and 76 males; 19 to 86 years of age; mean age: 51.7 +/- 15.7 years) participated in this study. Their self-efficacy was assessed using the self-efficacy scale for self-care (SESS) and the general self-efficacy scale (GSES) scores at the initial visit. SESS consists of three subscales: self-efficacy for dentist consultations, brushing of the teeth, and dietary habits. The subjects were classified into three groups: group 1 = 87 subjects who stayed in periodontal treatment and fulfilled maintenance criteria; group 2 = 17 subjects who stayed in periodontal treatment but did not fulfill criteria; and group 3 = 36 subjects who dropped out of periodontal treatment. Clinical and demographic characteristics and scores of GSES, SESS, and their subscales were compared among the three groups using the chi(2) and non-parametric multiple comparison tests.
    Results: The mean age of the subjects in groups I and 2 was significantly greater than the mean age of the subjects in group 3. Groups 1 and 2 exhibited significantly deeper probing depths and higher scores for SESS and its subscale, self-efficacy for dentist consultations, than did group 3.
    Conclusion: Assessment of oral health care-specific self-efficacy is effective for the prediction of patient completion of periodontal treatment.

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  • Contributions of social context to inequality in dental caries: a multilevel analysis of Japanese 3-year-old children

    J. Aida, Y. Ando, M. Oosaka, K. Niimi, M. Morita

    COMMUNITY DENTISTRY AND ORAL EPIDEMIOLOGY   36 ( 2 )   149 - 156   2008.4

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    Background: Community context as well as individual health behavior affects oral health status. However, the contribution of social context to dental caries among people in various regions remains unclear when individual health behavior is taken into account. Objectives: To determine the influence of community context on dmft among 3-year-old children. Methods: After all Japanese municipalities (n = 2522) had been stratified into nine regions with three caries levels, 44 municipalities were randomly selected. Community health service workers were asked to collect information on sociodemographic characteristics, oral health-related behavior, and dental condition for 3-year-old children during community dental health check-ups. Community-related variables, including socioeconomic status, social support, and social cohesion, were obtained from census data. Multilevel analysis was used to determine the effects of social context and individual behavior on dental caries. Results: A total of 3301 parents (79.9%) of 3-year-old children from 39 municipalities participated in our survey, and complete information was obtained from 3086 of them. Results of the analysis showed that 90.8% (P &lt; 0.001) of variance in dmft occurred at the individual level and that 9.2% (P &lt; 0.001) of the variance occurred at the community level. Individual-level variables explained only 6.6% of the individual level variance in dmft. Community-level variables explained 47.2% of the community level variance. Conclusions: There are statistically significant effects of social context on dmft in municipalities in Japan.

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  • Relationship between betel quid additives and established periodontitis among Bangladeshi subjects. International journal

    Rahena Akhter, Nur Mohammad Monsur Hassan, Jun Aida, Shuichi Takinami, Manabu Morita

    Journal of clinical periodontology   35 ( 1 )   9 - 15   2008.1

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    Aim: To determine the relationship between betel quid chewing additives and established periodontitis in Bangladeshi subjects.
    Material and Methods: A total of 864 subjects participated in this study. Among them, 140 pairs of sex- and age-matched case subjects and control subjects were selected. A case was defined as a person who had at least two sites with a clinical attachment level (CAL)&gt;= 6 mm and at least one site with probing depth (PD)&gt;= 5 mm. Subjects who did not fulfill these criteria were considered as controls. Information on sociodemographic variables, psychological stress, dental health behaviour, smoking and betel quid chewing habits was obtained.
    Results: Multiple logistic regression analysis showed that current betel quid chewers had greater probabilities of having established periodontal disease than did non-chewers (odds ratio=3.97, p &lt; 0.05). Mean PD, mean CAL, mean percentage of bleeding on probing and number of missing teeth were significantly higher in chewers of betel quid with tobacco and masala than in chewers of betel quid without such additives adjusting for age, sex, smoking habit, body mass index, dental visit pattern, stress and plaque index. Higher frequency and longer duration of betel quid chewing showed a significant relation to an increase in periodontal parameters.
    Conclusions: The results indicate that betel quid additives might significantly enhance periodontitis in the population studied.

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  • Behavior of in vitro, in vivo and internal motion of micro/nano particles of titanium, titanium oxides and others

    Fumio Watari, Shigeaki Abe, Chika Koyama, Atsuro Yokoyama, Tukasa Akasaka, Motohiro Uo, Makoto Matsuoka, Yasunori Totsuka, Mitsue Esaki, Manabu Morita, Tetsu Yonezawa

    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN   116 ( 1349 )   1 - 5   2008.1

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    To clarify the effect of micro/nanosizing of materials onto biological organism, the particle size dependence of reaction of cells and tissue as investigated by both biochemical cell functional test and animal implantation test. Especially for nanoparticles the behavior of invasion and internal diffusion inside body was visualized using an XSAM (X-ray Scanning Analytical Microscope). The increase of specific surface area is usually counted as nanosizing effect which causes the enhancement of chemical reactivity and therefore toxicity of materials such as carcinogenicity found in 500 nm Ni particles for the long term implantation in the soft tissue of rat. Even biocompatible materials such as Ti and TiO2 shows stimulus with the decrease of particle size. They cause phagocytosis to cells and inflammation to tissue when the size of particles is below 3 mu m. For the size below 50 nm, they may invade into the internal body through the respiratory or digestive system and diffuse inside body. After compulsory exposure test of 30 nm TiO2 particles through the respiratory system, the Ti mapping by XSAM showed the internal diffusion inside the whole body. Nanoparticles injected from caudal vein diffused with time course to lung, liver and spleen. The uptake of 30 nm TiO2 particles through the digestive system and diffusion into these organs was also confirmed. These phenomena observed in biocompatible or bioinert materials are the nonspecific, physical particle and shape effects which occur independent of materials. Nanoparticles might be the objects whose existence has not been assumed by the living body defense system.

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  • 臼歯部修復物の生存期間に関連する要因

    青山貴則, 相田 潤, 竹原順次, 森田 学

    口腔衛生学会雑誌   58 ( 1 )   16 - 24   2008

  • インプラント患者の口腔ケアを確かなものとするために

    森田 学

    日本歯科評論   68 ( 5 )   101   2008

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  • Vitamin C intake inhibits serum lipid peroxidation and osteoclast differentiation on alveolar bone in rats fed on a high-cholesterol diet.

    Toshihiro Sanbe, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

    Archives of Oral Biology   54 ( 3 )   235 - 240   2008

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  • フクロノリ抽出物およびリン酸一水素カルシウム配合キシリトール粒ガムの非う触誘発性の評価(6例での検討)

    三宅 亮, 森田 学

    健康・栄養食品研究   11 ( 1 )   1 - 8   2008

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  • Risk indicators for tooth loss due to caries and periodontal disease in recipients of free dental treatment in an adult population in Bangladesh. International journal

    Rahena Akhter, Nur Mohammad Monsur Hassan, Jun Aida, Khurshid Uz Zaman, Manabu Morita

    Oral health & preventive dentistry   6 ( 3 )   199 - 207   2008

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    Purpose: The aims of this study were first to identify the risk indicators for permanent tooth extraction in patients who were receiving free dental treatment, and second to determine whether or not the reasons for tooth extraction are related to socio-demographic factors.
    Materials and Methods: Bangladeshi adults who visited Dhaka Dental College Hospital participated in this study. For each extraction, the clinician recorded age, sex, educational status, type of tooth extracted, dietary habits, oral hygiene, history of smoking and betel quid chewing and reasons for tooth extraction. A series of bivariate analyses and logistic regression analyses were carried out to assess the effects of major variables.
    Results: A total of 868 teeth were extracted from 582 patients. Among them, 586 (67.5%) of the teeth were extracted due to caries and its sequelae, 161 (18.5%) and 121 (13.9%) were extracted for periodontal and other reasons. Logistic regression analysis revealed that tooth extraction due to caries had significant associations with age (P = 0.0001), tooth type (P = 0.013), consumption of sweets, snacks and soft drinks (P = 0.0001 and P = 0.0001, respectively), frequency of teeth cleaning (P = 0.007) and dental attendance pattern (P = 0.004). For tooth extraction due to periodontal disease, associations with age (P = 0.001), educational level (P = 0.018), tooth type (P = 0.024), betel quid chewing (P = 0.0001), smoking habit (P = 0.032), method of teeth cleaning (P = 0.001) and the use of dentifrices (P = 0.024) were statistically significant.
    Conclusions: In this group of patients, caries and its sequelae were the most common reasons for extraction of teeth, followed by periodontal disease. Betel quid chewing, smoking and dietary and oral hygiene habits were also significant predictors of tooth loss.

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  • 岡山大学医学部・歯学部附属病院の頭頚部癌チーム医療における予防歯科の関わり

    山中玲子, 水川展吉, 山本龍生, 小山玲子, 古田美智子, 江國大輔, 西川悟郎, 岡崎恵子, 志茂加代子, 羽川 操, 森田 学

    岡山歯学会雑誌   27 ( 2 )   93 - 98   2008

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  • 自立高齢者における歯牙欠損部の放置と栄養摂取状況との関連性

    秋野憲一, 相田 潤, 本多丘人, 森田 学

    北海道歯学雑誌   29 ( 2 )   159 - 168   2008

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  • 歯周基本治療の内容別にみた歯科医業収支の比較

    角舘直樹, 須貝 誠, 森田 学

    口腔衛生学会雑誌   58 ( 3 )   184 - 191   2008

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  • Association between experience of stressful life events and muscle-related temporomandibular disorders in patients seeking free treatment in a dental hospital. International journal

    R Akhter, N M Hassan, J Aida, T Kanehira, K U Zaman, Manabu Morita

    European journal of medical research   12 ( 11 )   535 - 40   2007.11

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    Objectives: Psychological factors are known to play an important role in the etiology and maintenance of temporomandibular disorders. Since there have been very few studies on this issue in Asian countries, the study was aimed to investigate the relationship between various stressful life events and temporomandibular disorders in patients seeking free treatment in a Dental Hospital, Bangladesh.
    Materials and Methods: Five hundred and twenty Bangladeshi adults (370 males and 150 females; mean age, 30.9 +/- 8.2 years) participated in this study. The subjects were given a questionnaire to evaluate their stress status in the last 12 months. The Research Diagnostic Criteria for TMD (RDC/TMD) was used as TMD diagnostic system by three standardized examiners. Two hundred and thirty-six patients were RDC/TMD-defined TMD-positive and were subsequently classified into 7 groups: group I, myofacial pain only; group 11, disk displacement only; group III, joint pain only; group IV, myofacial pain and disc displacement; group V, myofacial pain and joint pain; group VI, disc displacement and joint pain; and group VII, myofacial pain, disk displacement and joint pain. Two hundred and eighty-four subjects were RDC/TMD-defined TMD-negative subjects (controls). Adjusted odds ratios were calculated by multiple logistic regression analysis.
    Results: Logistic regression analysis revealed that patients diagnosed with myofacial pain (group 1) and a combination of myofacial and joint pain (group V) had significantly higher levels of financial and job stress than did the controls. Self-health-related stress and stress related to a spouse or deaths of a relative were also identified as predisposing factors for myofacial pain (group I).
    Conclusion: This study suggests that myofacial pain is more common in individuals with various types of psychological stress. When treating patients with facial pain, dentists should consider the possible presence of psychological factors.

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  • 3歳児う蝕有病者率の社会経済的状態に関連する地域格差は拡大傾向にある

    相田 潤, 森田 学, 安藤 雄一, アクター・ラヘナ, 小坂 健

    口腔衛生学会雑誌   57 ( 4 )   361 - 361   2007.8

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  • Toll-like receptor 3 ligand-induced antiviral response in mouse osteoblastic cells. International journal

    Kimiya Nakamura, Yoshiaki Deyama, Yoshitaka Yoshimura, Kuniaki Suzuki, Manabu Morita

    International journal of molecular medicine   19 ( 5 )   771 - 5   2007.5

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    Double-stranded RNA (dsRNA) and its mimic, polyinosinic acid:polycytidylic acid [poly(I):poly(C)], are recognized by toll-like receptor 3 (TLR3) that induces the production of IFN-beta in many cell types. In the present study, we investigated the effects of poly(I):poly(C) on mouse osteoblastic MC3T3-E1 (E1) cells. Poly(I):poly(C) markedly increased IFN-beta mRNA level in a dose-dependent manner. The increase in the IFN-beta mRNA level was apparent as early as 1 h after adding poly(I):poly(C) to the culture and peaked at 12 h. Stimulation with poly(I):poly(C) enhanced the expression of CXCL10 mRNA and TLR3 in E1 cells. Moreover, poly(I):poly(C) induced tyrosine phosphorylation of the transcription factor STAT1 in E1 cells. An anti-IFN-beta neutralizing antibody partially inhibited poly(I):poly(C)-induced CXCL10 mRNA, TLR3 mRNA and STAT1 phosphorylation. These results indicate that osteoblasts secrete IFN-beta in response to viral infection and that endogenous IFN-beta induces both CXCL10 and TLR3 production via an IFN-alpha/beta receptor-STAT1 pathway. It is suggested that osteoblasts are involved in host defense as well as bone metabolism.

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  • Dense fimbrial meshwork enhances Porphyromonas gingivalis adhesiveness: a scanning electron microscopic study

    H. Hongo, H. Takano, M. Morita

    JOURNAL OF PERIODONTAL RESEARCH   42 ( 2 )   114 - 118   2007.4

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    Background and Objective: The aim of this study was to determine how the fimbriae of Porphyromonas gingivalis function in plaque formation.
    Material and Methods: We used scanning electron microscopy to examine aggregates and hemaggregates of fimbria-rich ATCC33277 (parent) and fimbra-poor OZ6301C (pgmA-knockout, mutant) strains of P. gingivalis. We also assessed the hemagglutination activity of the two strains as an indicator of P. gingivalis adhesiveness.
    Results: Aggregates of P. gingivalis were composed of bacterial chains and clusters. Rich fimbriae projecting from cells of the parent strain tended to bunch and form a dense meshwork among bacterial cells. In contrast, cells of the mutant strain projected fewer fimbriae and the meshwork was looser. Hemaggregates including cells of the parent strain contained a detached, dense fimbrial meshwork that adhered to erythrocytes. Hemaggregates comprising cells of the mutant strain included bacterial chains and clusters that adhered to erythrocytes by shorter fimbriae than those of the parent strain. The hemagglutination titer of the parent strain was 10-fold higher than that of the mutant strain, although the number of fimbriae per cell of the parent strain was only double that of the mutant strain.
    Conclusion: The results indicate that P. gingivalis adhesiveness is prominently enhanced by the dense fimbrial meshwork. Thus, the virulence of P. gingivalis is increased by the presence of rich fimbriae.

    DOI: 10.1111/j.1600-0765.2006.00922.x

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  • 知的障害者施設利用者の口臭について

    江崎光恵, 相田 潤, 中村公也, 森田 学

    口腔衛生学会雑誌   57 ( 1 )   36 - 41   2007

  • Papillon-Lef&egrave;vre症候群と診断した姉妹例

    角舘直樹, 菅谷 勉, 小野芳男, 木村康一, 福島千之, 藤澤雅子, 永山正人, 森田 学, 野村和夫, 川浪雅光

    日本歯周病学会会誌   49 ( 3 )   239 - 249   2007

  • 非う蝕性歯頸部欠損と歯磨き習慣,咬合力,咬合接触面積および平均圧力との関係

    髙野知承, 竹原 順次, 森田 学

    口腔衛生学会雑誌   57 ( 5 )   613 - 621   2007

  • 歯科医院における歯冠修復処置と定期歯科健診の歯科医業収支の比較

    角舘直樹, 須貝 誠, 藤澤雅子, 森田 学

    口腔衛生学会雑誌   57 ( 5 )   640 - 649   2007

  • 歯周病患者のオーラルケアに対する自己効力感測定尺度の開発―信頼性と妥当性の検討―

    角舘直樹, 森田 学, 藤澤雅子, 永山正人, 川浪雅光

    日本歯周病学会会誌   49 ( 4 )   285 - 295   2007

  • 歯科衛生士における道徳的感性測定尺度の開発 〜信頼性と妥当性の検討

    藤澤雅子, 角舘直樹, 森田 学

    日本歯科医学教育学会雑誌   23 ( 3 )   289 - 298   2007

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  • 歯科疾患と「格差」に関する考察

    森田 学, 相田 潤

    日本歯科評論   67 ( 7 )   151 - 157   2007

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  • 女子中高生における口腔乾燥の自覚症状に関連する要因

    竹原順次, 中村公也, 三宅 亮, 森田 学

    口腔衛生学会雑誌   57 ( 2 )   111 - 116   2007

  • Relationship between salivary histatin 5 levels and Candida CFU counts in healthy elderly. International journal

    Jun Sugimoto, Takashi Kanehira, Hiroyuki Mizugai, Itsuo Chiba, Manabu Morita

    Gerodontology   23 ( 3 )   164 - 9   2006.9

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    OBJECTIVES: Few epidemiological studies have confirmed the antifungal activity of histatin 5 in saliva against Candida species. The purpose of this study was to examine the relationship between concentrations of histatin 5 and the number of cultivable Candida in saliva samples from elderly. METHODS: Whole saliva samples were obtained from 124 elderly people, 65 years or older, living in a rural community. The concentrations of histatin 5 in saliva samples were determined by the enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody. Total colony-forming units (CFUs) were counted on a selective medium for Candida. Multiple linear regression analysis was performed to determine the independent contribution of explanatory variables to Candida CFUs using age, sex, histatin 5 concentration and type of denture prosthesis as independent variables. RESULTS: Saliva samples from 104 subjects (84%) were candidal colony-positive. The youngest group (65-69 years old) showed significantly smaller Candida CFU counts than those in the older group. The mean Candida CFU count of denture wearers was significantly higher than that of non-denture wearers. Significantly negative associations were found between Candida CFU counts and histatin 5 level in the oldest group (p < 0.05) and in the full-denture wearers (p < 0.01). Multiple linear regression analysis revealed that Candida CFU counts were mostly associated with type of dentures, followed by histatin 5 concentration. CONCLUSION: Possible activity of histatin 5 against Candida in whole saliva of elderly people was epidemiologically confirmed. The area covered by the prostheses was a strong factor associated with the Candida CFU count.

    DOI: 10.1111/j.1741-2358.2006.00120.x

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  • Reasons for permanent tooth extractions in Japan.

    Jun Aida, Yuichi Ando, Rahena Akhter, Hitoshi Aoyama, Mineo Masui, Manabu Morita

    Journal of epidemiology   16 ( 5 )   214 - 9   2006.9

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    BACKGROUND: There has been no nationwide study in Japan on reasons for extraction of permanent teeth. This survey was aimed to determine the reasons for extraction of permanent teeth in Japan.
    METHODS: Five thousand, one hudred and thirty-one dentists were selected by systematic selection from the 2004 membership directory of the Japan Dental Association. The dentists selected were asked to record the reason for each extraction of permanent teeth during a period of one week from February 1 through 7, 2005. Reasons for tooth extraction were assigned to five groups: caries, fracture of teeth weakened by caries or endodontics, periodontal diseases, orthodontics, and other reasons.
    RESULTS: A total of 2,001 dentists (response rate of 39.1%) returned the questionnaires, and information on 9,115 extracted teeth from 7,499 patients was obtained. The results showed that caries and its sequela (totally 43.3%, 32.7% and 10.6%, respectively) and periodontal disease (41.8%) were the main reasons for teeth extraction. Extraction due to caries or fracture was commonly observed in all age groups over 15 years of age, whereas periodontal disease was predominant in the groups over 45 years of age.
    CONCLUSIONS: Most of the permanent teeth were extracted due to caries and its sequela and periodontal disease. Prevention and care for dental caries for all age groups and periodontal disease for over middle age groups are required.

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  • 年齢層別のヒト全唾液中のラクトフェリン量に関する研究

    兼平 孝, 高橋 大郎, 相田 潤, 森田 学

    口腔衛生学会雑誌   56 ( 4 )   548 - 548   2006.8

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  • 12歳児DMFTの減少に対するフッ化物洗口の影響の分析

    相田 潤, 森田 学, 安藤 雄一

    口腔衛生学会雑誌   56 ( 3 )   341 - 341   2006.7

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  • Comparison of antioxidant enzymes in saliva of elderly smokers and non-smokers. International journal

    Takashi Kanehira, Kouichi Shibata, Haruhiko Kashiwazaki, Nobuo Inoue, Manabu Morita

    Gerodontology   23 ( 1 )   38 - 42   2006.3

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    OBJECTIVES: This study was designed to compare the levels of copper/zinc superoxide dismutase (Cu/Zn SOD), peroxidase (POx) and glutathione peroxidase (GSH-Px) in saliva of smokers and those in saliva of non-smokers. METHODS: Unstimulated saliva samples were collected from 88 elderly males (65 years old or over) who visited a private dental clinic. Forty-four subjects were current smokers (more than 20 cigarettes daily for at least 30 years) and 44 were non-smokers. The levels of salivary thiocyanate, Cu/Zn SOD, GSH-Px, and POx activity were measured using standard procedures. RESULTS: The mean levels of salivary thiocyanate (SCN(-)) and SOD were significantly higher (p < 0.01) in the smoking group than in the non-smoking group, whereas the specific activity levels of POx and GSH-Px were significantly higher (p < 0.05) in the non-smoking group than in the smoking group. Significant correlation coefficients were found between the levels of SCN(-) and SOD (r = 0.37, p < 0.001). In the non-smoking group, a significant positive association was found between specific activity of POx and age (r = 0.33, p < 0.05). CONCLUSION: Measurement of SCN(-) and Cu/Zn SOD in human saliva might be useful for estimating the level of oxidative stress caused by cigarette smoke. Despite increased H2O2 level as a defense system induced by SOD, detoxification of H2O2 might be deteriorated in the oral cavity of elderly smokers.

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  • 口臭の自覚に関連した因子の検討

    高橋大郎, 相田 潤, 兼平 孝, 竹原順次, 森田 学

    口腔衛生学会雑誌   2006

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  • 歯科衛生士学校卒業時の学生が習得した態度・行動 〜「情意」という側面からの考察〜

    藤澤雅子, 森田 学, 熊澤隆樹, 角田裕子

    日本歯科医学教育学会雑誌   2006

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  • 永久歯の抜歯原因に関する全国調査

    森田 学

    8020推進財団会誌   2006

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  • 本学歯学部における「学生による臨床実習評価」の解析

    根岸 淳, 宇野 滋, 飯塚 正, 佐藤嘉晃, 山本恒之, 加我正行, 森田 学, 吉田重光

    日本歯科医学教育学会雑誌   2006

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  • An ecological study on the association of public dental health activities and sociodemographic characteristics with caries prevalence in Japanese 3-year-old children

    J. Aida, Y. Ando, H. Aoyama, T. Tango, M. Morita

    CARIES RESEARCH   40 ( 6 )   466 - 472   2006

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    The aim of this ecological study was to determine the association of the frequency of dental health activities conducted as public health service and sociodemographic characteristics with caries prevalence in Japanese 3-year-old children using data for each municipality as one unit. Data on caries prevalence in 3-year-old children in 2000 were obtained from 3,251 municipalities ( almost all municipalities in Japan). Caries prevalence for each municipality was recalculated using the empirical Bayes estimation model to make adjustments for variations in municipalities with small numbers of children. Explanatory factors included annual frequency of dental health activities such as dental health education and topical fluoride application programs and various sociodemographic characteristics. Multiple linear regression analysis was applied to examine the associations between caries prevalence and annual frequency of dental health activities with adjustment of other sociodemographic variables. Variations in caries prevalence in municipalities with small populations became smaller after being adjusted by the empirical Bayes estimation model. Multiple linear regression analysis showed significant associations of caries prevalence with sociodemographic characteristics such as the proportion of residents with university degrees, total fertility rate, and the proportion of workers in the manufacturing industry (p &lt; 0.001). Annual frequency of topical fluoride application showed a significant but relatively weak association with caries prevalence (p &lt; 0.001), explaining only 0.8% of the total variation in caries prevalence, whereas no correlation was found between caries prevalence and annual frequency of dental health education. It was concluded that the effect of the fluoride application service on caries prevalence in 3-year-old children, although statistically significant, was of far lower clinical significance than sociodemographic characteristics. Copyright (c) 2006 S. Karger AG, Basel

    DOI: 10.1159/000095644

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  • 女子中高生における顎機能異常に関連する要因分析 共分散構造分析を用いての検討

    三宅 亮, 新里勝宏, 竹原順次, 中村公也, 森田 学

    口腔衛生学会雑誌   2006

  • Relationship between stress factor and periodontal disease in a rural area population in Japan

    R Akhter, MA Hannan, R Okhubo, M Morita

    EUROPEAN JOURNAL OF MEDICAL RESEARCH   10 ( 8 )   352 - 357   2005.8

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    Objectives: Several studies conducted in Western countries have shown significant associations between stress factors and periodontal disease. However, there have been only a few studies conducted in Asian countries. The present study was designed to identify possible relationship between stress and periodontal disease in residents of a rural area in Japan.
    Material and Methods: Data were collected from 1,089 adults with at least six natural teeth in a typical farming district of Japan. Subjects were asked to complete a questionnaire on daily stresses in various aspects of life. Data on gender, occupation, smoking, alcohol drinking habits, dental health behavior and systemic disease status were obtained from the questionnaires. Periodontal disease status was assessed using clinical attachment loss (CAL), and the subjects were dichotomized according to mean CAL &lt;1.5 mm (control group) and &gt;= 1.5 mm (diseased group). Logistic regression was applied to assess the associations between stress and other factors with periodontal disease, and odds ratios (ORs) as well as 95% confidence intervals were calculated. Results: In bivariate analysis, significant relationships were found between periodontal disease and stress within 1 month (P&lt;0.001), job stress (P&lt;0.001), self-health-related stress (P &lt;0.001) and family health-related stress (P&lt;0.01). Logistic regression analysis revealed that subjects who felt job stress (OR = i.71, P&lt;0.05) and those who felt stress due to self health (OR = 1.72, P&lt;0.05) were more prone to have periodontal disease than were those who never or only rarely felt such stress. Significant correlations were also found between periodontal disease and smoking habit, frequency of dental clinic visits and hyperlipidemia (OR = 1.8, P&lt;0.05, OR 2.0, P&lt;0.001, OR = 2.1, P&lt;0.05, respectively).
    Conclusion: The results suggest that stress related to self health and job might be potential risk indicators for development of periodontal diseases. Intervention measures including stress reduction may provide adjunctive approaches for preventing and treating periodontal disease.

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  • Formation and resolution of ankylosis under application of recombinant human bone morphogenetic protein-2 (rhBMP-2) to class III furcation defects in cats

    D Takahashi, T Odajima, M Morita, M Kawanami, H Kato

    JOURNAL OF PERIODONTAL RESEARCH   40 ( 4 )   299 - 305   2005.8

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    Objectives: Periodontal regeneration under application of bone morphogenetic protein (BMP) is compromised by ankylosis. Ankylosis disappearance following application of BMP has been observed in the case of a small defect, which might be beneficial change for periodontal regeneration. However, the histological observation of ankylosis disappearance has not been demonstrated in a large defect. The purpose of this present study was to confirm resolution of ankylosis during periodontal regeneration by recombinant human BMP-2 (rhBMP-2) applied to class III furcation defects.
    Material and Methods: Class III furcation defects were created in the premolars of six adult cats. The rhBMP-2 material, prepared by applying rhBMP-2 to a combination of polylactic acid-polygricolic copolymer and gelatin sponge (PGS; 0.33 mu g rhBMP-2/mm(3) PGS) or control material containing only PGS, was implanted into each defect. The cats were killed at 3, 6 or 12 weeks after surgery and serial sections were prepared for histological and histometrical observation.
    Results: Ankylosis was observed in some of the rhBMP-2/PGS group at 3 and 6 weeks, but not at 12 weeks. At 6 weeks, osteoclast-like cells were visible in the rhBMP-2/PGS group with ankylosis. Residual PGS was evident between the bone and root surface in the rhBMP-2/PGS group without ankylosis at 3 weeks.
    Conclusions: Resolution of ankylosis by osteoclast-like cells possibly occurred under application of rhBMP-2. Residual PGS might play an important role in preventing ankylosis formation.

    DOI: 10.1111/j.1600-0765.2005.00794.x

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  • 食事調査を用いた3歳児のう蝕に関連する保健行動評価の質問紙の作成

    相田 潤, 新美 完, 森田 学

    口腔衛生学会雑誌   55 ( 4 )   439 - 439   2005.8

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  • 永久歯の抜歯原因に関する全国実態調査(第1報) 基礎統計量の報告

    新美 完, 相田 潤, 安藤 雄一, 青山 旬, 増井 峰夫, アクター・ラヘナ, 大坂 美希子, 森田 学

    口腔衛生学会雑誌   55 ( 4 )   398 - 398   2005.8

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  • 永久歯の抜歯原因に関する全国実態調査(第2報) 歯の喪失状態に関する全国推計の試み

    青山 旬, 安藤 雄一, 相田 潤, 増井 峰夫, 森田 学

    口腔衛生学会雑誌   55 ( 4 )   404 - 404   2005.8

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  • 歯科修復物の生存時間分析

    青山 貴則, 相田 潤, 森田 学

    口腔衛生学会雑誌   55 ( 2 )   129 - 129   2005.4

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  • Rare earth oxide-containing fluorescent glass filler for composite resin.

    Motohiro Uo, Mayumi Okamoto, Fumio Watari, Kazutoshi Tani, Manabu Morita, Akira Shintani

    Dental materials journal   24 ( 1 )   49 - 52   2005.3

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    In recent dental care, esthetic restorative materials such as composite resin and porcelain have been widely used and studied. However, their good esthetics makes the visual inspection of restored teeth difficult. In this study, a fluorescent glass filler containing rare earth oxides - which are well-known fluorescent materials - was prepared and used in composite resin to enhance visual inspection capability with small change in color. For example, when irradiated with near ultraviolet light, an Eu-2(3)2(3)O(-containing filler fluoresced clearly and visibly. The fluorescence intensity of the prepared composite resin increased with increase of Eu)O (content in the glass filler and with filler/resin ratio in the resin. Despite the clear fluorescence, the effect of Eu)(O)(2)(3) (cal the color change of composite resin was quite small - even when up to 10 wt% Eu)(O)(2)(3) (was added to the glass filler. Tb)(O)(- and Dy)(O)(-added fillers also showed clear fluorescence, just like Eu)(O)(4)(7)(2)(3)(2)(3)-added filler. Therefore, fluorescent glass fillers for composite resins, resulting in small color change in the latter, were successfully prepared in this study.

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  • 口臭の自覚と関連した因子の検討

    高橋 大郎, 相田 潤, 兼平 孝, 竹原 順次, 森田 学

    日本歯周病学会会誌   47 ( 春季特別 )   127 - 127   2005.3

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  • Relationships between lifestyle and dental health behaviors in a rural population in Japan. International journal

    Shoji Harada, Rahena Akhter, Keiko Kurita, Miyako Mori, Misuzu Hoshikoshi, Hidehiko Tamashiro, Manabu Morita

    Community dentistry and oral epidemiology   33 ( 1 )   17 - 24   2005.2

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    Objectives: The aim of this study was to determine associations of lifestyle with dental health behaviors such as tooth brushing frequency, use of extra cleaning devices, and regular dental visits to a dentist. Methods: Data were collected from 1182 dentate residents 18 years of age or older who resided in a typical farming district. The data included data on the demographic factors, dental health behavior, and various aspects of lifestyle, i.e. mental condition, alcohol consumption, smoking habit, physical activity, social activity, dietary habits, and presence of systemic diseases. Results: Multiple logistic regression analysis revealed that subjects in a younger group (18-39 years of age) and subjects who had never smoked brushed their teeth more frequently. Experience of social volunteer work and presence of systemic disease were correlated with use of extra cleaning devices. Associations of female gender with frequency of tooth brushing and use of extra cleaning devices were weakly positive. The subjects who considered dietary combination carefully and those who lived alone were predisposed to visit a dentist regularly. Conclusions: The results indicate that dental health behavior is associated with lifestyle as well as demographic factors.

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  • う蝕のない3歳児の割合の増加と地域要因との関連-北海道212市町村での地域相関研究

    柄崎哲郎, 相田 潤, 本多丘人, 森田 学

    北海道歯学雑誌   2005

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  • 高齢喫煙者の唾液中の抗酸化酵素に関する研究

    柴多浩一, 兼平 孝, 柏崎晴彦, 森田 学, 井上農夫男

    北海道歯学雑誌   2005

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  • Bite force and its association with signs of temporomandibular disorder in Bangladeshi adolescents

    Rahena Akhter, Hayato Nameki, Abdul Hannan, Kimiya Nakamura, Okahito Honda, Manabu Morita

    International Journal of Oral Health   2005

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  • ネットワークを利用した歯学教材システムの構築と有用性の検討

    飯塚 正, 宇尾基弘, 向後隆男, 森田 学

    北海道歯学雑誌   2005

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  • 経験的Bayes推定値を用いた市町村別3歳児う蝕有病者率の地域比較および歯科保健水準との関連

    相田 潤, 安藤 雄一, 青山 旬, 丹後 俊郎, 森田 学

    口腔衛生学会雑誌   54 ( 4 )   361 - 361   2004.8

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  • 高齢者の口腔乾燥症に関する調査

    兼平 孝, 水谷 博幸, 村松 真澄, 相田 潤, 千葉 逸朗, 森田 学

    口腔衛生学会雑誌   54 ( 4 )   404 - 404   2004.8

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  • The effect of duration and force of mechanical toothbrushing stimulation on proliferative activity of the junctional epithelium. International journal

    Takaaki Tomofuji, Manabu Morita, Masazumi Horiuchi, Tomonori Sakamoto, Daisuke Ekuni, Tatsuo Yamamoto, Tatsuo Watanabe

    Journal of periodontology   73 ( 10 )   1149 - 52   2002.10

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    BACKGROUND: Gingival bleeding is frequently accompanied by an ulcerated epithelium, with repair depending on the proliferative activity of the epithelial cells. The present study examined the proliferative activity of the junctional epithelium (JE) under several different methods of toothbrushing stimulation. METHODS: Twelve dogs were used in this 3-week experiment. Prior to the experiment, all teeth underwent daily removal of plaque and calculus using a scaler. Teeth were then divided into quadrants: 9 teeth in 3 quadrants (test, 3 per quadrant) were mechanically stimulated by toothbrushing for various time intervals (10, 20, or 40 seconds) and at various forces (100, 200, or 250 gf). The 3 teeth in the fourth quadrant served as controls. The proliferative activity of the basal cells of the junctional epithelium was evaluated for expression of proliferating cell nuclear antigen (PCNA). The numbers of PCNA-positive basal cells and total basal cells were counted, and the width of the junctional epithelium was measured. RESULTS: Toothbrushing force significantly affected the PCNA-positive basal cell ratio (P < 0.05). The 200 g toothbrushing force produced the highest PCNA-positive basal cell ratio, which was significantly higher than that using the 100 g force (P < 0.05). CONCLUSION: In junctional epithelium stimulated with a toothbrush, the PCNA-positive basal cell ratio is more sensitive to toothbrushing force than to duration.

    DOI: 10.1902/jop.2002.73.10.1149

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  • Toothbrushing promotes gingival fibroblast proliferation more effectively than removal of dental plaque. International journal

    Masazumi Horiuchi, Tatsuo Yamamoto, Takaaki Tomofuji, Akira Ishikawa, Manabu Morita, Tatsuo Watanabe

    Journal of clinical periodontology   29 ( 9 )   791 - 5   2002.9

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    OBJECTIVES: Removal of dental plaque is an essential element of periodontal treatment. However, there have also been studies of the effects of the mechanical stimulation provided by toothbrushing on gingival host-defense mechanisms. The aim of the study was to evaluate the effects of toothbrushing on gingival fibroblast proliferation in dogs over time, compared to effects of plaque removal without brushing. METHODS: The mouths of six mongrel dogs were divided into four quadrants: two for daily toothbrushing, and two for daily plaque removal with a curette. After 1, 3 and 5 weeks of treatment, histometrical analyses were performed to assess inflammatory cell infiltration, proliferating cell nuclear antigen (PCNA)-positive fibroblasts, procollagen type I-positive fibroblasts in the subepithelial connective tissue of junctional epithelium. RESULTS: Toothbrushing increased the number of PCNA-positive fibroblasts in the first week, increased the number of type I procollagen-positive fibroblasts at the fifth week, and reduced inflammatory cell infiltration at the third week. CONCLUSION: These findings suggest that mechanically stimulated fibroblasts begin proliferating within a week, and this cell division results in an increased number of fibroblasts at the third week. It takes 5 weeks before differences in collagen synthesis between brushing and plaque removal areas are detectable.

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  • ナイロンに多元素鉱物粉状体を混合して形成したフィラメントを用いた歯ブラシのブラッシング効果.

    坂本友紀, 友藤孝明, 堀内正純, 江國大輔, 森田 学, 山本龍生, 渡邊達夫

    口腔衛生学会雑誌   2002

  • Effect of <Capnocytophaga gingivalis>___- on Calcium Phosphate Precipitation Inhibitors

    MORITA Manabu, YAMAMOTO Tatsuo, WATANABE Tatsuo

    Journal of the Japanese Association of Periodontology   33   1991.8

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    Publisher:The Japanese Society of Periodontology  

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  • 永久歯の萌出時期,萌出順序の経年的変化に関する研究

    山本龍生, 森田学, 渡邊達夫

    口腔衛生学会雑誌   41 ( 1 )   23 - 34   1991

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Presentations

  • 職業階層。勤務形態と歯周疾患との関連

    職域における歯科口腔保健の今後の課題と今後の展望  2017 

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  • Oral Health promotion Myannmar

    Myanmar Health Reseach Congress  2015 

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  • Effects of Coenzyme Q10 on Wound Healing After Tooth Extraction

    IADR General Session  2015 

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  • Self-efficacy Related to Periodontal Diseases: A 3-Year Longitudinal Study

    IADR General Session  2015 

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  • Relationship Between Periodontitis and Pancreatic Cancer

    IADR General Session  2015 

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  • 歯周状態と全身性のストレスとの横断的な関連.

    第87回 日本産業衛生学会  2014 

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  • 糖尿病と口腔保健支援型の関連瀬尾の検討

    第73回日本公衆衛生学会  2014 

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  • 近年における永久歯の萌出年齢の推移

    第73回日本公衆衛生学会  2014 

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  • 若年者の早食いは肥満のリスクになる

    第73回日本公衆衛生学会  2014 

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  • チーム医療や腫瘍センターにおける専門的口腔ケアに関するアンケート調査

    第25回 近畿・中国・四国 口腔衛生学会総会  2014 

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  • 化学療法中のポストポリオ症候群患者に対して口腔衛生管理を行った一症例

    第25回 近畿・中国・四国 口腔衛生学会総会  2014 

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  • 岡山市内の小学校児童における矮小歯の発生頻度

    第25回 近畿・中国・四国 口腔衛生学会総会  2014 

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  • 岡山大学病院周術期管理センターにおける術前プラークフリーの実際

    第25回 近畿・中国・四国 口腔衛生学会総会  2014 

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  • 還元型コエンザイムQ10の抜歯窩創傷治癒への効果の検討

    第25回 近畿・中国・四国 口腔衛生学会総会  2014 

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  • 勝英支所管内における歯科保健の現状と学齢期の歯と口の健康づくり事業について

    第60回中国地区公衆衛生学会  2014 

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  • 咬合異常によるストレスはアポリポタンパクE欠損ラットの動脈硬化を促進する

    日本口腔衛生学会・総会  2014 

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  • 口腔常在菌叢による宿主防御機構への影響

    日本口腔衛生学会・総会  2014 

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  • 周病による全身の酸化ストレスへの影響:酸化ストレス可視化マウスを用いた検討.

    日本口腔衛生学会・総会  2014 

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  • アルコール摂取後のブラッシング行動と歯周病の活動性との関連

    日本口腔衛生学会・総会  2014 

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  • 若年者における血圧と歯周炎の関連についてのコホート研究

    日本口腔衛生学会・総会  2014 

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  • 口腔常在菌叢による歯周組織への影響

    日本歯周病学会春季学術大会  2014 

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  • 職域成人における口腔内所見および口腔保健行動とメタボリックシンドロームとの関係

    第87回 日本産業衛生学会  2014 

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  • 医療連携担当衛生士としての活動

    第33回岡山歯学会  2012 

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  • 岡山大学新入生自覚的口腔健康観に影響する因子の検討

    第33回岡山歯学会  2012 

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  • 歯科における禁煙診療の標準化と教育・研修モデル

    第22回 日本歯科医学会総会  2012 

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  • 日本とカンボジアの若年者におけるソーシャル・キャピタルと口腔の健康の関係

    第71回日本公衆衛生学会総会  2012 

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  • 岡山市内3小学校の児童における永久歯萌出状況の報告

    第23回 近畿・中国・四国口腔衛生学会  2012 

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  • Change in time of permanent teeth eruption in Japanese children.

    10th AAPD meeting  2012 

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  • 歯科医師臨床研修におけるチーム医療教育

    第31回日本歯科医学教育学会  2012 

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  • 歯周病対策としての水素水の有効性

    第14回関西ウォーター研究会講演会  2012 

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  • Effects of self-efficacy on oral health behaviors and gingival health in young people

    Europerio 7  2012 

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  • コーヒー摂取と歯周病の関連

    第61回日本口腔衛生学会・総会  2012 

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  • 実験的歯周炎に対する水素水摂取の予防効果の検証

    第2回分子状水素医学シンポジウム  2012 

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  • Association between parental smoking and dental health care behavior

    IADR meeting  2010 

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  • Oral Malodorous Compound Induces Osteoclast Differentiation without RANKL

    IADR meeting  2010 

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  • Relationship between Decrease of Erythrocytes and Progression of Periodontal Disease

    IADR meeting,  2010 

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  • Occlusal Disharmony Affects BDNF Levels in Rat Submandibular Gland.

    IADR meeting  2010 

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  • 歯科保健指導が肥満に及ぼす効果-観音寺市特定保健指導の介入調査-

    第21回 近畿・中国・四国 口腔衛生学会総会  2010 

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  • 大学生における不正咬合とストレスの関係

    第21回 近畿・中国・四国 口腔衛生学会総会  2010 

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  • 美作保健所における三歳児う蝕に関する問題点

    日本プライマリ・ケア学会岡山支部学術大会  2010 

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  • 美作保健所における三歳児のう蝕状況の経年的変化

    第16回岡山県保健福祉学会  2010 

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  • Comparison of Health Behaviors Associated to Periodontitis among Young Adults with Different BMI Levels.

    The Joint Scientific Meeting of the International Epidemiological Association Western Pacific Region and the Japan Epidemiological Association  2010 

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  • The Parents Smoking and Social Environment: Multilevel Analysis.

    The Joint Scientific Meeting of the International Epidemiological Association Western Pacific Region and the Japan Epidemiological Association,  2010 

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  • 音楽の聴取による生体への影響~唾液中のストレスマーカーを指標として~

    第58回日本口腔衛生学会・総会.岐阜  2009 

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  • 口臭原因物質の破骨細胞RAW264の分化に及ぼす影響

    第58回日本口腔衛生学会・総会  2009 

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  • 肥満モデルラットにおける動脈のインシュリン抵抗性に関する歯周炎の影響

    第58回日本口腔衛生学会・総会  2009 

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  • メインテナンス期の歯周病患者において咬合力は予後因子となりうる

    第58回日本口腔衛生学会・総会  2009 

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  • 食器の共用や食物の口移しを注意すれば,う蝕は予防できるのか?

    第58回日本口腔衛生学会・総会  2009 

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  • Oral Care for Patients with Head and Neck Cancers.

    World Congress on Preventive Dentistry  2009 

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  • Development of the Outcome Expectancy Scale for Self-care

    World Congress on Preventive Dentistry  2009 

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  • Development of the Outcome Expectancy Scale for Self-care.

    World Congress on Preventive Dentistry  2009 

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  • Association between Neighborhood-level Socioeconomic Status and Oral Health Behavior

    World Congress on Preventive Dentistry,  2009 

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  • Cocoa-enriched diet decreases gingival oxidative stress in rat periodontitis

    Annual Meeting of the Society of Free Radical Research – Europe  2009 

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  • Risk factors for temporomandibular disorders: a 3-year prospective cohort study

    The IADR 87th General Session & Exhibition.  2009 

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  • Effects of dental health programs on medical expenses in municipalities

    The IADR 87th General Session & Exhibition  2009 

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  • Relationship between Labial Closure Force and Visual Acuity in Japanese Elementary School Children

    First Asia-Pacific Conference on Health Promotion and Education (APHPE)  2009 

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  • インテナンス期の歯周炎患者における咬合力と歯周状態の関係について

    .第20回日本口腔衛生学会近畿・中国・四国地方会総会  2009 

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  • 急性期病院における頭頸部癌患者への口腔ケア

    第20回日本口腔衛生学会近畿・中国・四国地方会総会  2009 

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  • 特定健康診査・特定保健指導における歯周病予防対策の可能性 –医療保険者による生活習慣病対策の導入をめぐって-

    日本歯周病学会  2009 

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  • 液体歯磨剤「クリーンデンタル薬用リンス」の抗炎症・抗菌成分のラット歯周炎モデルへの効果

    第51回春季日本歯周病学会  2009 

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  • Hydrogen sulfide application induces a transient osteoclast differentiation with RANKL expression in the rat model

    International Conference on Breath and Breath Odor Research  2009 

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  • 全国規模の疫学調査からみえてくるもの-う蝕の地域格差等-

    岡山歯学会総会・学術集会  2009 

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  • 歯周病患者における咬合力と歯周状態の関係について

    第30回岡山歯学会総会・学術集会  2009 

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  • 大学新入生における歯周疾患と口腔保健行動との関係および口腔保健行動の性差

    第58回日本口腔衛生学会・総会  2009 

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  • 3歳児の不正咬合とう蝕有病,食べ方,体格について

    第58回日本口腔衛生学会・総会  2009 

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  • マウスガードの効果について

    第58回日本口腔衛生学会・総会  2009 

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  • Effect of non-surgical periodontal therapy on plasma reactive oxygen metabolites.

    第56回JADR総会・学術大会  2008 

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  • Effects of Obesity on Oxidative Stress in Rat Gingiva.

    第56回JADR総会・学術大会  2008 

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  • A new portable monitor for measurement of odorous compounds in oral, exhales and nasal air

    The 8 th International Conference of Asian Academy of Preventive Dentistry  2008 

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  • Oral Health education for patients with periodontitis

    Symposium of 8 th International Conference of Asian Academy of Preventive Dentistry  2008 

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  • 歯周炎モデルラットにおける歯肉酸化ストレス度・抗酸化力に対するビタミンC全身投与の効果

    第57回日本口腔衛生学会  2008 

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  • 職場における歯科保健-生活習慣病予防における歯科の役割-

    第18回日本産業衛生学会 産業医・産業看護全国協議会  2008 

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  • 高コレステロール食によるラットの血清過酸化脂質の増加と歯槽骨における破骨細胞の分化:ビタミンC摂取の影響

    第29回 岡山歯学会総会・学術集会  2008 

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  • 岡山大学病院における頭頸部癌チーム医療と予防歯科の関わり

    第29回 岡山歯学会総会・学術集会  2008 

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  • アンケートによる歯周疾患スクリーニングの有効性

    第29回 岡山歯学会総会・学術集会  2008 

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  • 処置別にみた歯科医業収支の比較

    第21回日本歯科医学会総会  2008 

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  • Relationship between force lip closure and visual acunity

    The 8 th International Conference of Asian Academy of Preventive Dentistry  2008 

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  • Relationship between peridontitis and hepatic abnormalities in male university students Japan

    The 8 th International Conference of Asian Academy of Preventive Dentistry  2008 

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  • 社会的勾配と口腔保健

    日本産業衛生学会  2008 

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  • 口腔保健の評価法-成人期の歯科保健・予防対策・集団歯科保健の状況把握・効果の評価-.

    第81回日本産業衛生学会  2008 

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  • 咬合力・咬合接触面積と各種運動能力・健康診断結果との関連について

    第57回日本口腔衛生学会  2008 

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  • 新入生の歯科健診とアンケート調査 -第2報-歯周病のスクリーニング検査としてのアンケートの有効性-

    第57回日本口腔衛生学会  2008 

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  • 新入生の歯科健診とアンケート調査 -第1報-新入生の口腔内状態とアンケート結果の概要

    第57回日本口腔衛生学会  2008 

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  • 北海道における8020運動に基づく歯と全身の健康に関する実態調査

    第57回日本口腔衛生学会  2008 

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  • 小児患者のオーラルケアに対する母親の自己効力感測定尺度の開発 ー信頼性と妥当性の検討ー

    第57回日本口腔衛生学会  2008 

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  • 骨芽細胞様MC3T3-E1細胞におけるRIG-Iの発現

    歯科基礎医学会学術大会  2008 

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  • Relationship of Periodontal Status and Physical Fitness in Bangladeshi population

    The IADR 86th General Session & Exhibition  2008 

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  • Relationship between folic acid intake and gingival health in non-smokers

    The IADR 86th GeneralSession & Exhibition  2008 

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  • Relationship of oral health status and physical fitness in Bangladeshi population

    第19回日本老年歯科医学会総会・学術大会  2008 

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  • 簡易型口腔乾燥症判定キットの開発

    第19回日本老年歯科医学会総会・学術大会  2008 

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  • 統合失調症患者における口腔ケア介入研究 第2報 唾液ストレスマーカーについて

    第19回日本老年歯科医学会総会・学術大会  2008 

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  • 青春期以降のむし歯と歯周病の予防

    北海道子供の歯を守る会道民公開講座  2008 

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  • セルフケア促進のための患者教育の方法に関する研究-自己効力理論の応用

    第51回日本歯周病学会  2008 

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  • 歯周初期治療における歯科衛生士業務の内容別にみた歯科医業収支の比較

    日本口腔衛生学会北海道地方会  2008 

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  • 中学生2年生にみられた上顎第一、第二大臼歯埋伏の1例 -学校歯科健診の観点から-

    日本口腔衛生学会北海道地方会  2008