2025/08/19 更新

写真a

オオウチ　ヒデヨ
大内　淑代
OHUCHI Hideyo
所属
医歯薬学域 教授
職名
教授
外部リンク

研究キーワード

  • 線維芽細胞増殖因子10

  • 細胞増殖

  • 器官形成

  • 短波長オプシン

  • 細胞分化

  • マウス

  • 眼発生、網膜発生

  • 形態形成

  • 疾患モデルの作製と解析

  • 組織形態計測

  • 組織修復

  • iPS細胞

  • 光受容細胞の細胞組織学・発生

  • ハーダー腺

  • 上皮と腺

  • 涙腺

研究分野

  • ライフサイエンス / 解剖学  / 細胞組織学

  • ライフサイエンス / 発生生物学

 

論文

  • Direct photoreception by pituitary endocrine cells regulates hormone release and pigmentation. 査読 国際誌

    Ayaka Fukuda, Keita Sato, Chika Fujimori, Takahiro Yamashita, Atsuko Takeuchi, Hideyo Ohuchi, Chie Umatani, Shinji Kanda

    Science (New York, N.Y.)   387 ( 6729 )   43 - 48   2025年1月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    The recent discovery of nonvisual photoreceptors in various organs has raised expectations for uncovering their roles and underlying mechanisms. In this work, we identified a previously unrecognized hormone-releasing mechanism in the pituitary of the Japanese rice fish (medaka) induced by light. Ca2+ imaging analysis revealed that melanotrophs, a type of pituitary endocrine cell that secretes melanocyte-stimulating hormone, robustly increase the concentration of intracellular Ca2+ during short-wavelength light exposure. Moreover, we identified Opn5m as the key molecule that drives this response. Knocking out opn5m attenuated melanogenesis by reducing tyrosinase expression in the skin. Our findings suggest a mechanism in which direct reception of short-wavelength light by pituitary melanotrophs triggers a pathway that might contribute to protection from ultraviolet radiation in medaka.

    DOI： 10.1126/science.adj9687

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  • Histological differences related to autophagy in the minor salivary gland between primary and secondary types of Sjögren's syndrome. 査読 国際誌

    Hitomi Ono-Minagi, Tsutomu Nohno, Kiyofumi Takabatake, Takehiro Tanaka, Takayuki Katsuyama, Kohta Miyawaki, Jun Wada, Soichiro Ibaragi, Seiji Iida, Tadashi Yoshino, Hitoshi Nagatsuka, Takayoshi Sakai, Hideyo Ohuchi

    BMC oral health   24 ( 1 )   1099 - 1099   2024年9月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    Some forms of Sjögren's syndrome (SS) follow a clinical course accompanied by systemic symptoms caused by lymphocyte infiltration and proliferation in the liver, kidneys, and other organs. To better understand the clinical outcomes of SS, here we used minor salivary gland tissues from patients and examine their molecular, biological, and pathological characteristics. A retrospective study was performed, combining clinical data and formalin-fixed paraffin-embedded (FFPE) samples from female patients over 60 years of age who underwent biopsies at Okayama University Hospital. We employed direct digital RNA counting with nCounter® and multiplex immunofluorescence analysis with a PhenoCycler™ on the labial gland biopsies. We compared FFPE samples from SS patients who presented with other connective tissue diseases (secondary SS) with those from stable SS patients with symptoms restricted to the exocrine glands (primary SS). Secondary SS tissues showed enhanced epithelial damage and lymphocytic infiltration accompanied by elevated expression of autophagy marker genes in the immune cells of the labial glands. The close intercellular distance between helper T cells and B cells positive for autophagy-associated molecules suggests accelerated autophagy in these lymphocytes and potential B cell activation by helper T cells. These findings indicate that examination of FFPE samples from labial gland biopsies can be an effective tool for evaluating molecular histological differences between secondary and primary SS through multiplexed analysis of gene expression and tissue imaging.

    DOI： 10.1186/s12903-024-04869-4

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  • Harderian Gland Development and Degeneration in the Fgf10-Deficient Heterozygous Mouse. 査読 国際誌

    Shiori Ikeda, Keita Sato, Hirofumi Fujita, Hitomi Ono-Minagi, Satoru Miyaishi, Tsutomu Nohno, Hideyo Ohuchi

    Journal of developmental biology   12 ( 2 )   2024年6月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    The mouse Harderian gland (HG) is a secretory gland that covers the posterior portion of the eyeball, opening at the base of the nictitating membrane. The HG serves to protect the eye surface from infection with its secretions. Mice open their eyelids at about 2 weeks of age, and the development of the HG primordium mechanically opens the eye by pushing the eyeball from its rear. Therefore, when HG formation is disturbed, the eye exhibits enophthalmos (the slit-eye phenotype), and a line of Fgf10+/- heterozygous loss-of-function mice exhibits slit-eye due to the HG atrophy. However, it has not been clarified how and when HGs degenerate and atrophy in Fgf10+/- mice. In this study, we observed the HGs in embryonic (E13.5 to E19), postnatal (P0.5 to P18) and 74-week-old Fgf10+/- mice. We found that more than half of the Fgf10+/- mice had markedly degenerated HGs, often unilaterally. The degenerated HG tissue had a melanized appearance and was replaced by connective tissue, which was observed by P10. The development of HGs was delayed or disrupted in the similar proportion of Fgf10+/- embryos, as revealed via histology and the loss of HG-marker expression. In situ hybridization showed Fgf10 expression was observed in the Harderian mesenchyme in wild-type as well as in the HG-lacking heterozygote at E19. These results show that the Fgf10 haploinsufficiency causes delayed or defective HG development, often unilaterally from the unexpectedly early neonatal period.

    DOI： 10.3390/jdb12020016

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  • The Germinal Origin of Salivary and Lacrimal Glands and the Contributions of Neural Crest Cell-Derived Epithelium to Tissue Regeneration 査読

    Hitomi Ono-Minagi, Tsutomu Nohno, Takashi Serizawa, Yu Usami, Takayoshi Sakai, Hideyuki Okano, Hideyo Ohuchi

    International Journal of Molecular Sciences   24 ( 18 )   13692 - 13692   2023年9月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MDPI AG  

    The vertebrate body comprises four distinct cell populations: cells derived from (1) ectoderm, (2) mesoderm, (3) endoderm, and (4) neural crest cells, often referred to as the fourth germ layer. Neural crest cells arise when the neural plate edges fuse to form a neural tube, which eventually develops into the brain and spinal cord. To date, the embryonic origin of exocrine glands located in the head and neck remains under debate. In this study, transgenic TRiCK mice were used to investigate the germinal origin of the salivary and lacrimal glands. TRiCK mice express fluorescent proteins under the regulatory control of Sox1, T/Brachyury, and Sox17 gene expressions. These genes are representative marker genes for neuroectoderm (Sox1), mesoderm (T), and endoderm (Sox17). Using this approach, the cellular lineages of the salivary and lacrimal glands were examined. We demonstrate that the salivary and lacrimal glands contain cells derived from all three germ layers. Notably, a subset of Sox1-driven fluorescent cells differentiated into epithelial cells, implying their neural crest origin. Also, these Sox1-driven fluorescent cells expressed high levels of stem cell markers. These cells were particularly pronounced in duct ligation and wound damage models, suggesting the involvement of neural crest-derived epithelial cells in regenerative processes following tissue injury. This study provides compelling evidence clarifying the germinal origin of exocrine glands and the contribution of neural crest-derived cells within the glandular epithelium to the regenerative response following tissue damage.

    DOI： 10.3390/ijms241813692

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  • Mammalian type Opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response. 査読 国際誌

    Keita Sato, Takahiro Yamashita, Hideyo Ohuchi

    The Journal of biological chemistry   105020 - 105020   2023年7月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    Mammalian type Opsin 5 (Opn5m), a UV-sensitive GPCR opsin highly conserved in vertebrates, would provide a common basis for UV sensing from lamprey to humans. However, G protein coupled with Opn5m remains controversial due to variations in assay conditions and the origin of Opn5m across different reports. Here, we examined Opn5m from diverse species using an aequorin luminescence assay and Gα-knockout cell line. Beyond the commonly studied major Gα classes, Gαq, Gα11, Gα14, and Gα15 in the Gq class were individually investigated in this study, as they can drive distinct signaling pathways in addition to a canonical calcium response. UV light triggered a calcium response via all the tested Opn5m proteins in 293T cells, which was abolished by Gq-type Gα deletion and rescued by co-transfection with mouse and medaka Gq-type Gα proteins. Opn5m preferentially activated Gα14 and close relatives. Mutational analysis implicated specific regions, including α3-β5 and αG-α4 loops, αG and α4 helices, and the extreme C-terminus, in the preferential activation of Gα14 by Opn5m. Fluorescent in situ hybridization revealed co-expression of genes encoding Opn5m and Gα14 in the scleral cartilage of medaka and chicken eyes, supporting their physiological coupling. This suggests that the preferential activation of Gα14 by Opn5m is relevant for UV sensing in specific cell types.

    DOI： 10.1016/j.jbc.2023.105020

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  • The medaka mutant deficient in eyes shut homolog exhibits opsin transport defects and enhanced autophagy in retinal photoreceptors 査読 国際誌

    Keita Sato, Yang Liu, Takahiro Yamashita, Hideyo Ohuchi

    Cell Tissue Res   391 ( 2 )   249 - 267   2023年2月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    Eyes shut homolog (EYS) encodes a proteoglycan and the human mutation causes retinitis pigmentosa type 25 (RP25) with progressive retinal degeneration. RP25 most frequently affects autosomal recessive RP patients with many ethnic backgrounds. Although studies using RP models have facilitated the development of therapeutic medications, Eys has been lost in rodent model animals. Here we examined the roles for Eys in the maintenance of photoreceptor structure and function by generating eys-null medaka fish using the CRISPR-Cas9 system. Medaka EYS protein was present near the connecting cilium of wild-type photoreceptors, while it was absent from the eys-/- retina. The mutant larvae exhibited a reduced visual motor response compared with wild-type. In contrast to reported eys-deficient zebrafish at the similar stage, no retinal cell death was detected in the 8-month post-hatching (8-mph) medaka eys mutant. Immunohistochemistry showed a significant reduction in the length of cone outer segments (OSs), retention of OS proteins in the inner segments of photoreceptors, and abnormal filamentous actin network at the base of cone OSs in the mutant retina by 8 mph. Electron microscopy revealed aberrant structure of calyceal processes, numerous vesiculation and lamellar interruptions, and autophagosomes in the eys-mutant cone photoreceptors. In situ hybridization showed an autophagy component gene, gabarap, was ectopically expressed in the eys-null retina. These results suggest eys is required for regeneration of OS, especially of cone photoreceptors, and transport of OS proteins by regulating actin filaments. Enhanced autophagy may delay the progression of retinal degeneration when lacking EYS in the medaka retina.

    DOI： 10.1007/s00441-022-03702-0

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  • Protrusion of KCNJ13 Gene Knockout Retinal Pigment Epithelium Due to Oxidative Stress–Induced Cell Death 査読 国際誌

    Yuki Kanzaki, Hirofumi Fujita, Keita Sato, Mio Hosokawa, Hiroshi Matsumae, Yuki Morizane, Hideyo Ohuchi

    Investigative Opthalmology and Visual Science   63 ( 12 )   29 - 29   2022年11月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Association for Research in Vision and Ophthalmology (ARVO)  

    PURPOSE: This study was performed to elucidate the mechanisms of morphological abnormalities in a Leber congenital amaurosis 16 (LCA16) cell model using KCNJ13 knockout (KO) retinal pigment epithelial cells derived from human iPS cells (hiPSC-RPE). METHODS: In KCNJ13 KO and wild-type hiPSC-RPE cells, ZO-1 immunofluorescence was performed, and confocal images were captured. The area and perimeter of each cell were measured. To detect cell death, ethidium homodimer III (EthD-III) staining and LDH assay were used. Scanning electron microscopy (SEM) was used to observe the cell surface. The expression levels of oxidative stress-related genes were examined by quantitative PCR. To explore the effects of oxidative stress, tert-butyl hydroperoxide (t-BHP) was administered to the hiPSC-RPE cells. Cell viability was tested by MTS assay, whereas oxidative damage was monitored by oxidized (GSSG) and reduced glutathione levels. RESULTS: The area and perimeter of KCNJ13-KO hiPSC-RPE cells were enlarged. EthD-III-positive cells were increased with more dead cells in the protruded region. The KO RPE had significantly higher LDH levels in the medium. SEM observations revealed aggregated cells having broken cell surfaces on the KO RPE sheet. The KCNJ13-deficient RPE showed increased expression levels of oxidative stress-related genes and total glutathione levels. Furthermore, t-BHP induced a significant increase in cell death and GSSG levels in the KO RPE. CONCLUSIONS: We suggest that in the absence of the Kir.7.1 potassium channel, human RPE cells are vulnerable to oxidative stress and ultimately die. The dying/dead cells form aggregates and protrude from the surviving KCNJ13-deficient RPE sheet.

    DOI： 10.1167/iovs.63.12.29

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  • The Opsin 3/Teleost multiple tissue opsin system: mRNA localization in the retina and brain of medaka (Oryzias latipes) 査読 国際誌

    Sato K, Nwe Nwe K, Ohuchi H

    J Comp Neurol   529 ( 10 )   2484 - 2516   2021年1月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    The photoreceptor protein, opsin, is one of the major components for vision and photoreceptive function in animals. Although many opsins have been discovered from animal genomes, only a few non-image-forming functions mediated by opsins have been identified. Understanding the mRNA distribution of photoreceptor proteins is one crucial step in uncovering their photoreceptive function in animals. Here we focus on the medaka fish (Oryzias latipes) Opsin 3 (Opn3)/Teleost multiple opsin (Tmt) system, which constitutes a separate phylogenetic group, having putative blue light photoreceptors for non-image-forming functions. In medaka, there is one opn3 and five tmt-opsin orthologs. The expression pattern of the opn3/tmt-opsins in the retina and brain was investigated by in situ hybridization. mRNAs for opn3/tmt-opsins were distributed in the retinal ganglion cells as well as interneurons and specific brain nuclei. Specifically, hybridization signals were observed in the glutamate decarboxylase 1 (gad1)-expressing amacrine cells for opn3, tmt1a, tmt1b, and tmt2, in the caudal lobe of the cerebellum for tmt1b and tmt2, in the cranial nerve nuclei for opn3, tmt1a, tmt1b, tmt2, and in the rostral pars distalis (adenohypophysis) for opn3. These expression patterns suggest that blue light sensing in the fish retina and brain may be involved in the integration of visual inputs, vestibular function, somatosensation, motor outputs, and pituitary endocrine regulation. This article is protected by copyright. All rights reserved.

    DOI： 10.1002/cne.25106

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  • Fgf10-CRISPR mosaic mutants demonstrate the gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung 査読 国際誌

    Munenori Habuta, Akihiro Yasue, Ken-ichi T. Suzuki, Hirofumi Fujita, Keita Sato, Hitomi Kono, Ayuko Takayama, Tetsuya Bando, Satoru Miyaishi, Seiichi Oyadomari, Eiji Tanaka, Hideyo Ohuchi

    PLOS ONE   15 ( 10 )   e0240333 - e0240333   2020年10月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Public Library of Science (PLoS)  

    CRISPR/Cas9-mediated gene editing often generates founder generation (F0) mice that exhibit somatic mosaicism in the targeted gene(s). It has been known that Fibroblast growth factor 10 (Fgf10)-null mice exhibit limbless and lungless phenotypes, while intermediate limb phenotypes (variable defective limbs) are observed in the Fgf10-CRISPR F0 mice. However, how the lung phenotype in the Fgf10-mosaic mutants is related to the limb phenotype and genotype has not been investigated. In this study, we examined variable lung phenotypes in the Fgf10-targeted F0 mice to determine if the lung phenotype was correlated with percentage of functional Fgf10 genotypes. Firstly, according to a previous report, Fgf10-CRISPR F0 embryos on embryonic day 16.5 (E16.5) were classified into three types: type I, no limb; type II, limb defect; and type III, normal limbs. Cartilage and bone staining showed that limb truncations were observed in the girdle, (type I), stylopodial, or zeugopodial region (type II). Deep sequencing of the Fgf10-mutant genomes revealed that the mean proportion of codons that encode putative functional FGF10 was 8.3 ± 6.2% in type I, 25.3 ± 2.7% in type II, and 54.3 ± 9.5% in type III (mean ± standard error of the mean) mutants at E16.5. Histological studies showed that almost all lung lobes were absent in type I embryos. The accessory lung lobe was often absent in type II embryos with other lobes dysplastic. All lung lobes formed in type III embryos. The number of terminal tubules was significantly lower in type I and II embryos, but unchanged in type III embryos. To identify alveolar type 2 epithelial (AECII) cells, known to be reduced in the Fgf10-heterozygous mutant, immunostaining using anti-surfactant protein C (SPC) antibody was performed: In the E18.5 lungs, the number of AECII was correlated to the percentage of functional Fgf10 genotypes. These data suggest the Fgf10 gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung. Since dysfunction of AECII cells has been implicated in the pathogenesis of parenchymal lung diseases, the Fgf10-CRISPR F0 mouse would present an ideal experimental system to explore it.

    DOI： 10.1371/journal.pone.0240333

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  • KCNJ13 Gene Deletion Impairs Cell Alignment and Phagocytosis in Retinal Pigment Epithelium Derived from Human-Induced Pluripotent Stem Cells 査読 国際誌

    Yuki Kanzaki, Hirofumi Fujita, Keita Sato, Mio Hosokawa, Hiroshi Matsumae, Fumio Shiraga, Yuki Morizane, Hideyo Ohuchi

    Investigative Opthalmology & Visual Science   61 ( 5 )   38 - 38   2020年5月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Association for Research in Vision and Ophthalmology (ARVO)  

    Purpose: The purpose of this study was to establish and analyze a cell model of Leber congenital amaurosis type 16 (LCA16), which is caused by mutations in the KCNJ13 gene encoding Kir7.1, an inward-rectifying potassium ion channel. Methods: The two guide RNAs specific to the target sites in the KCNJ13 gene were designed and KCNJ13 knock-out (KO) human-induced pluripotent stem cells (hiPSCs) were generated using the CRISPR/Cas9 system. The KCNJ13-KO hiPSCs were differentiated into retinal pigment epithelial cells (hiPSC-RPEs). The KCNJ13-KO in hiPSC-RPEs was confirmed by immunostaining. Phagocytic activity of hiPSC-RPEs was assessed using the uptake of fluorescently labeled porcine photoreceptor outer segments (POSs). Phagocytosis-related genes in RPE cells were assessed by quantitative polymerase chain reaction. Results: Most of the translated region of the KCNJ13 gene was deleted in the KCNJ13-KO hiPSCs by the CRISPR/Cas9 system, and this confirmed that the Kir7.1 protein was not present in RPE cells induced from the hiPSCs. Expression of RPE marker genes such as BEST1 and CRALBP was retained in the wild-type (WT) and in the KCNJ13-KO hiPSC-RPE cells. However, phagocytic activity and expression of phagocytosis-related genes in the KCNJ13-null hiPSC-RPE cells were significantly reduced compared to those of WT. Conclusions: We succeeded in generating an RPE model of LCA16 using hiPSCs. We suggest that Kir7.1 is required for phagocytosis of POSs by RPE cells and that impaired phagocytosis in the absence of Kir7.1 would be involved in the retinal degeneration found in LCA16.

    DOI： 10.1167/iovs.61.5.38

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  • Localization of the ultraviolet-sensor Opn5m and its effect on myopia-related gene expression in the late-embryonic chick eye. 査読 国際誌

    Kato M, Sato K, Habuta M, Fujita H, Bando T, Morizane Y, Shiraga F, Miyaishi S, Ohuchi H

    Biochemistry and biophysics reports   19   100665 - 100665   2019年9月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.bbrep.2019.100665

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  • Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor 査読

    Keita Sato, Takahiro Yamashita, Hideyo Ohuchi, Atsuko Takeuchi, Hitoshi Gotoh, Katsuhiko Ono, Misao Mizuno, Yasuhisa Mizutani, Sayuri Tomonari, Kazumi Sakai, Yasushi Imamoto, Akimori Wada, Yoshinori Shichida

    Nature Communications   9 ( 1 )   1255   2018年12月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Nature Publishing Group  

    DOI： 10.1038/s41467-018-03603-3

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  • Relationship between somatic mosaicism of Pax6 mutation and variable developmental eye abnormalities-an analysis of CRISPR genome-edited mouse embryos 査読

    Akihiro Yasue, Hitomi Kono, Munenori Habuta, Tetsuya Bando, Keita Sato, Junji Inoue, Seiichi Oyadomari, Sumihare Noji, Eiji Tanaka, Hideyo Ohuchi

    Scientific Reports   7 ( 1 )   53   2017年

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Nature Publishing Group  

    DOI： 10.1038/s41598-017-00088-w

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  • Two Opsin 3-Related Proteins in the Chicken Retina and Brain: A TMT-Type Opsin 3 Is a Blue-Light Sensor in Retinal Horizontal Cells, Hypothalamus, and Cerebellum 査読

    Mutsuko Kato, Takashi Sugiyama, Kazumi Sakai, Takahiro Yamashita, Hirofumi Fujita, Keita Sato, Sayuri Tomonari, Yoshinori Shichida, Hideyo Ohuchi

    PLOS ONE   11 ( 11 )   e0163925   2016年11月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1371/journal.pone.0163925

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  • Evolution of Mammalian Opn5 as a Specialized UV-absorbing Pigment by a Single Amino Acid Mutation 査読

    Takahiro Yamashita, Katsuhiko Ono, Hideyo Ohuchi, Akane Yumoto, Hitoshi Gotoh, Sayuri Tomonari, Kazumi Sakai, Hirofumi Fujita, Yasushi Imamoto, Sumihare Noji, Katsuki Nakamura, Yoshinori Shichida

    JOURNAL OF BIOLOGICAL CHEMISTRY   289 ( 7 )   3991 - 4000   2014年2月

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    担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1074/jbc.M113.514075

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  • A Non-Mammalian Type Opsin 5 Functions Dually in the Photoreceptive and Non-Photoreceptive Organs of Birds 査読

    Hideyo Ohuchi, Takahiro Yamashita, Sayuri Tomonari, Sari Fujita-Yanagibayashi, Kazumi Sakai, Sumihare Noji, Yoshinori Shichida

    PLOS ONE   7 ( 2 )   e31534   2012年2月

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    担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1371/journal.pone.0031534

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  • Opn5 is a UV-sensitive bistable pigment that couples with Gi subtype of G protein 査読

    Takahiro Yamashita, Hideyo Ohuchi, Sayuri Tomonari, Keiko Ikeda, Kazumi Sakai, Yoshinori Shichida

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   107 ( 51 )   22084 - 22089   2010年12月

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    担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1073/pnas.1012498107

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  • Expression patterns of the opsin 5-related genes in the developing chicken retina 査読

    Sayuri Tomonari, Kyoichi Migita, Akira Takagi, Sumihare Noji, Hideyo Ohuchi

    DEVELOPMENTAL DYNAMICS   237 ( 7 )   1910 - 1922   2008年7月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/dvdy.21611

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  • A dual role of FGF10 in proliferation and coordinated migration of epithelial leading edge cells during mouse eyelid development 査読

    H Tao, M Shimizu, R Kusumoto, K Ono, S Noji, H Ohuchi

    DEVELOPMENT   132 ( 14 )   3217 - 3230   2005年7月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1242/dev.01892

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  • Mutations in the gene encoding fibroblast growth factor 10 are associated with aplasia of lacrimal and salivary glands 査読

    M Entesarian, H Matsson, J Klar, B Bergendal, L Olson, R Arakaki, Y Hayashi, H Ohuchi, B Falahat, AI Bolstad, R Jonsson, M Wahren-Herlenius, N Dahl

    NATURE GENETICS   37 ( 2 )   125 - 127   2005年2月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/ng1507

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  • FGF10 acts as a major ligand for FGF receptor 2 IIIb in mouse multi-organ development 査読

    H Ohuchi, Y Hori, M Yamasaki, H Harada, K Sekine, S Kato, N Itoh

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   277 ( 3 )   643 - 649   2000年11月

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    担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • Fgf10 is essential for limb and lung formation 査読

    Keisuke Sekine, Hideyo Ohuchi, Masanori Fujiwara, Masahiro Yamasaki, Tatsuya Yoshizawa, Takashi Sato, Naoko Yagishita, Daisuke Matsui, Yoshihiko Koga, Nobuyuki Itoh, Shigeaki Kato

    Nature Genetics   21 ( 1 )   138 - 141   1999年1月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/5096

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  • The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor 査読

    H. Ohuchi, T. Nakagawa, A. Yamamoto, A. Araga, T. Ohata, Y. Ishimaru, H. Yoshioka, T. Kuwana, T. Nohno, M. Yamasaki, N. Itoh, S. Noji

    Development   124 ( 11 )   2235 - 2244   1997年

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    担当区分：筆頭著者   記述言語：英語  

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  • A repertoire of visible light-sensitive opsins in the deep-sea hydrothermal vent shrimp Rimicaris hybisae. 査読 国際誌

    Yuya Nagata, Norio Miyamoto, Keita Sato, Yosuke Nishimura, Yuki Tanioka, Yuji Yamanaka, Susumu Yoshizawa, Kuto Takahashi, Kohei Obayashi, Hisao Tsukamoto, Ken Takai, Hideyo Ohuchi, Takahiro Yamashita, Yuki Sudo, Keiichi Kojima

    The Journal of biological chemistry   301 ( 7 )   110291 - 110291   2025年7月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    Unlike terrestrial environments, where humans reside, there is no sunlight in the deep sea. Instead, dim visible light from black-body radiation and bioluminescence illuminates hydrothermal vent areas in the deep sea. A deep-sea hydrothermal vent shrimp, Rimicaris hybisae, is thought to detect this dim light using its enlarged dorsal eye; however, the molecular basis of its photoreception remains unexplored. Here, we characterized the molecular properties of opsins, universal photoreceptive proteins in animals, found in R. hybisae. Transcriptomic analysis identified six opsins: three Gq-coupled opsins, one Opn3, one Opn5, and one peropsin. Functional analysis revealed that five of these opsins exhibited light-dependent G protein activity, whereas peropsin exhibited the ability to convert all-trans-retinal to 11-cis-retinal like photoisomerases. Notably, all the R. hybisae opsins, including Opn5, convergently show visible light sensitivity (around 457-517 nm), whereas most opsins categorized as Opn5 have been demonstrated to be UV sensitive. Mutational analysis revealed that the unique visible light sensitivity of R. hybisae Opn5 is achieved through the stabilization of a protonated Schiff base by a counterion residue at position 83 (Asp83), which differs from the position identified in other opsins. These findings suggest that the vent shrimp R. hybisae has adapted its photoreceptive devices to dim deep-sea hydrothermal light by selectively maintaining a repertoire of visible light-sensitive opsins, including the uniquely tuned Opn5.

    DOI： 10.1016/j.jbc.2025.110291

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  • What is the identity of Gerota fascia? Histological study with cadavers. 査読 国際誌

    Yasuyuki Kobayashi, Kohei Edamura, Takuya Sadahira, Yusuke Tominaga, Satoshi Katayama, Takehiro Iwata, Shingo Nishimura, Tomoko Kobayashi, Keita Sato, Takaaki Komiyama, Ryusuke Momota, Hideyo Ohuchi, Motoo Araki

    International journal of urology : official journal of the Japanese Urological Association   2024年10月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    OBJECTIVES: The advancement of laparoscopic surgery has allowed surgeons to see finer anatomical structures during surgery. As a result, several issues have arisen regarding Gerota fascia that cannot be explained by previous interpretations, such as its various forms observed during surgery. To address these issues, we histologically examined the structure of Gerota fascia. METHODS: Specimens for study were prepared from kidneys with Gerota fascia from four cadavers, and the structure was studied histologically. Its thickness and collagen fiber area ratios were measured using ImageJ and compared to those of the epimysium of the rectus abdominis muscle. RESULTS: Connective tissue that appeared to be Gerota fascia was observed in 26 specimens. Histologically, the basic structure of Gerota fascia was a sandwich-like structure with a thin layer of thick, long collagen fibers in the central layer, and small granular collagen fibers scattered at the edges. However, not all areas observed had a similar structure; eight specimens were composed only of small granular collagen fibers. The average thickness of the Gerota fascia was 466 μm, and the area ratio of collagen was 27.1%. In contrast, the epimysium was much thicker than Gerota fascia, and its collagen fibers were much thicker and denser. CONCLUSIONS: Gerota fascia, unlike the epimysium, was a very thin and fragile layer of collagen fibers, and its structure was diverse. This explains why Gerota fascia was observed in various states during surgery. It is important for surgeons to understand the properties of Gerota fascia and to treat it appropriately.

    DOI： 10.1111/iju.15596

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  • Functional diversification process of opsin genes for teleost visual and pineal photoreceptions. 査読 国際誌

    Chihiro Fujiyabu, Fuki Gyoja, Keita Sato, Emi Kawano-Yamashita, Hideyo Ohuchi, Takehiro G Kusakabe, Takahiro Yamashita

    Cellular and molecular life sciences : CMLS   81 ( 1 )   428 - 428   2024年10月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Science and Business Media LLC  

    Abstract

    Most vertebrates have a rhodopsin gene with a five-exon structure for visual photoreception. By contrast, teleost fishes have an intron-less rhodopsin gene for visual photoreception and an intron-containing rhodopsin (exo-rhodopsin) gene for pineal photoreception. Here, our analysis of non-teleost and teleost fishes in various lineages of the Actinopterygii reveals that retroduplication after branching of the Polypteriformes produced the intron-less rhodopsin gene for visual photoreception, which converted the parental intron-containing rhodopsin gene into a pineal opsin in the common ancestor of the Teleostei. Additional analysis of a pineal opsin, pinopsin, shows that the pinopsin gene functions as a green-sensitive opsin together with the intron-containing rhodopsin gene for pineal photoreception in tarpon as an evolutionary intermediate state but is missing in other teleost fishes, probably because of the redundancy with the intron-containing rhodopsin gene. We propose an evolutionary scenario where unique retroduplication caused a “domino effect” on the functional diversification of teleost visual and pineal opsin genes.

    DOI： 10.1007/s00018-024-05461-3

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    その他リンク： https://link.springer.com/article/10.1007/s00018-024-05461-3/fulltext.html

  • Molecular Property, Manipulation, and Potential Use of Opn5 and Its Homologs. 招待 査読 国際誌

    Keita Sato, Hideyo Ohuchi

    Journal of molecular biology   168319 - 168319   2023年10月

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    担当区分：最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    Animal opsin is a G-protein coupled receptor (GPCR) and binds retinal as a chromophore to form a photopigment. The Opsin 5 (Opn5) group within the animal opsin family comprises a diverse array of related proteins, such as Opn5m, a protein conserved across all vertebrate lineages including mammals, and other members like Opn5L1 and Opn5L2 found in non-mammalian vertebrate genomes, and Opn6 found in non-therian vertebrate genomes, along with Opn5 homologs present in invertebrates. Although these proteins collectively constitute a single clade within the molecular phylogenetic tree of animal opsins, they exhibit markedly distinct molecular characteristics in areas such as retinal binding properties, photoreaction, and G-protein coupling specificity. Based on their molecular features, they are believed to play a significant role in physiological functions. However, our understanding of their precise physiological functions and molecular characteristics is still developing and only partially realized. Furthermore, their unique molecular characteristics of Opn5-related proteins suggest a high potential for their use as optogenetic tools through more specialized manipulations. This review intends to encapsulate our current understanding of Opn5, discuss potential manipulations of its molecular attributes, and delve into its prospective utility in the burgeoning field of animal opsin optogenetics.

    DOI： 10.1016/j.jmb.2023.168319

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  • Diversification processes of teleost intron-less opsin genes. 査読 国際誌

    Chihiro Fujiyabu, Keita Sato, Hideyo Ohuchi, Takahiro Yamashita

    The Journal of biological chemistry   104899 - 104899   2023年6月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    Opsins are universal photosensitive proteins in animals. Vertebrates have a variety of opsin genes for visual and non-visual photoreceptions. Analysis of the gene structures shows that most opsin genes have introns in their coding regions. However, teleosts exceptionally have several intron-less opsin genes which are presumed to have been duplicated by an RNA-based gene duplication mechanism, retroduplication. Among these retrogenes, we focused on the Opn4 (melanopsin) gene responsible for non-image-forming photoreception. Many teleosts have five Opn4 genes including one intron-less gene, which is speculated to have been formed from a parental intron-containing gene in the Actinopterygii. In this study, to reveal the evolutionary history of Opn4 genes, we analyzed them in teleost (zebrafish and medaka) and non-teleost (bichir, sturgeon and gar) fishes. Our synteny analysis suggests that the intron-less Opn4 gene emerged by retroduplication after branching of the bichir lineage. In addition, our biochemical and histochemical analyses showed that, in the teleost lineage, the newly acquired intron-less Opn4 gene became abundantly used without substantial changes of the molecular properties of the Opn4 protein. This stepwise evolutionary model of Opn4 genes is quite similar to that of rhodopsin genes in the Actinopterygii. The unique acquisition of rhodopsin and Opn4 retrogenes would have contributed to the diversification of the opsin gene repertoires in the Actinopterygii and the adaptation of teleosts to various aquatic environments.

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  • Chromophore Structure in an Inactive State of a Novel Photosensor Protein Opn5L1: Resonance Raman Evidence for the Formation of a Deprotonated Adduct at the 11th Carbon Atom. 査読 国際誌

    Misao Mizuno, Keita Sato, Takahiro Yamashita, Kazumi Sakai, Yasushi Imamoto, Yumiko Yamano, Akimori Wada, Hideyo Ohuchi, Yoshinori Shichida, Yasuhisa Mizutani

    The journal of physical chemistry. B   127 ( 10 )   2169 - 2176   2023年3月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    Opsins are photosensitive G protein-coupled receptor proteins and are classified into visual and nonvisual receptors. Opn5L1 is a nonvisual opsin that binds all-trans retinal as a chromophore. A unique feature of Opn5L1 is that the protein exhibits a photocyclic reaction upon photoexcitation. Determining the chromophore structures of intermediates in the photocycle is essential for understanding the functional mechanism of Opn5L1. A previous study revealed that a long-lived intermediate in the photocycle cannot activate the G protein and forms a covalent bond between the retinal chromophore and a nearby cysteine residue. However, the position of this covalent bond in the chromophore remains undetermined. Here, we report a resonance Raman study on isotopically labeled samples in combination with density functional theory calculations and reveal that the 11th carbon atom of the chromophore of the intermediate forms a covalent linkage to the cysteine residue. Furthermore, vibrational assignments based on the isotopic substitutions and density functional theory calculations suggested that the Schiff base of the intermediate is deprotonated. The chromophore structure determined in the present study well explains the mechanism of the photocyclic reaction, which is crucial to the photobiological function of Opn5L1.

    DOI： 10.1021/acs.jpcb.2c08780

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  • Evaluation of oral care using MA-T gel for high-risk patients: a pilot study 査読 国際誌

    Hitomi Ono-Minagi, Nao Gojo, Tsutomu Nohno, Tsuyoshi Inoue, Hideyo Ohuchi, Takayoshi Sakai

    BMC Oral Health   23 ( 1 )   108 - 108   2023年2月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Science and Business Media {LLC}  

    <jats:title>Abstract</jats:title><jats:sec>
    <jats:title>Background</jats:title>
    <jats:p>Oral care with gel is a common method for preventing aspiration in high-risk patients. An oral care gel is used to clean and moisturize the oral cavity. However, the effects of gel care on the oral bacteria remain unclear. In this pilot study, we described a matching transformation system (MA-T) for elderly high-risk patients. MA-T is an on-demand aqueous chlorine dioxide solution that provides excellent safety and has various antimicrobial activities, even in the presence of abundant organic compounds. This study investigated the effects of MA-T gel in patients requiring nursing care.</jats:p>
    </jats:sec><jats:sec>
    <jats:title>Materials and methods</jats:title>
    <jats:p>Patients who were hospitalized for nursing care were included in this study. No drugs and foods were administered orally. Oral bacteria and intraoral humidity were examined by daily care using MA-T gel. Moreover, oral membranous substances were analyzed and material from the oral cavity was cultured on selective media for identifying opportunistic organisms.</jats:p>
    </jats:sec><jats:sec>
    <jats:title>Results</jats:title>
    <jats:p>Membranous substances were present in the oral cavities of all patients. The number of bacteria decreased, and oral moisture improved, after treatment with MA-T gel. Moreover, oral humidity was also controlled with the continued use of MA-T gel. MA-T gels should be used not only for professional care but also on a daily basis for better oral care. Furthermore, the results of bacterial cultures showed that MA-T controls the propagation of opportunistic bacterial infections.</jats:p>
    </jats:sec><jats:sec>
    <jats:title>Conclusion</jats:title>
    <jats:p>Membranous substances may be observed in the oral cavity of individuals requiring nursing care for tube feeding. The results of this pilot study suggest that MA-T, a novel disinfectant, can be used for oral care in the elderly to reduce the risk of aspiration-pneumonia.</jats:p>
    </jats:sec>

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  • Cysteinyl leukotriene receptor 1 is dispensable for osteoclast differentiation and bone resorption 査読 国際誌

    Hirofumi Fujita, Aoi Ando, Yohei Mizusawa, Mitsuaki Ono, Takako Hattori, Munenori Habuta, Toshitaka Oohashi, Satoshi Kubota, Hideyo Ohuchi

    PLOS ONE   17 ( 11 )   e0277307 - e0277307   2022年11月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Public Library of Science (PLoS)  

    Cysteinyl leukotriene receptor 1 (CysLTR1) is a G protein-coupled receptor for the inflammatory lipid mediators cysteinyl leukotrienes, which are involved in smooth muscle constriction, vascular permeability, and macrophage chemokine release. The Cysltr1 gene encoding CysLTR1 is expressed in the macrophage lineage, including osteoclasts, and the CysLTR1 antagonist Montelukast has been shown to suppress the formation of osteoclasts. However, it currently remains unclear whether CysLTR1 is involved in osteoclast differentiation and bone loss. Therefore, to clarify the role of CysLTR1 in osteoclastogenesis and pathological bone loss, we herein generated CysLTR1 loss-of-function mutant mice by disrupting the cysltr1 gene using the CRISPR-Cas9 system. These mutant mice had a frameshift mutation resulting in a premature stop codon (Cysltr1 KO) or an in-frame mutation causing the deletion of the first extracellular loop (Cysltr1Δ105). Bone marrow macrophages (BMM) from these mutant mice lost the intracellular flux of calcium in response to leukotriene D4, indicating that these mutants completely lost the activity of CysLTR1 without triggering genetic compensation. However, disruption of the Cysltr1 gene did not suppress the formation of osteoclasts from BMM in vitro. We also demonstrated that the CysLTR1 antagonist Montelukast suppressed the formation of osteoclasts without functional CysLTR1. On the other hand, disruption of the Cysltr1 gene partially suppressed the formation of osteoclasts stimulated by leukotriene D4 and did not inhibit that by glutathione, functioning as a substrate in the synthesis of cysteinyl leukotrienes. Disruption of the Cysltr1 gene did not affect ovariectomy-induced osteoporosis or lipopolysaccharide-induced bone resorption. Collectively, these results suggest that the CysLT-CysLTR1 axis is dispensable for osteoclast differentiation in vitro and pathological bone loss, while the leukotriene D4-CysTR1 axis is sufficient to stimulate osteoclast formation. We concluded that the effects of glutathione and Montelukast on osteoclast formation were independent of CysLTR1.

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  • Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium 招待 査読 国際誌

    Toshiki Kinuhata, Keita Sato, Tetsuya Bando, Taro Mito, Satoru Miyaishi, Tsutomu Nohno, Hideyo Ohuchi

    Journal of Developmental Biology   10 ( 4 )   45 - 45   2022年10月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MDPI AG  

    The first event of differentiation and morphogenesis in the optic vesicle (OV) is specification of the neural retina (NR) and retinal pigment epithelium (RPE), separating the inner and outer layers of the optic cup, respectively. Here, we focus on a basic helix-loop-helix gene, BHLHE40, which has been shown to be expressed by the developing RPE in mice and zebrafish. Firstly, we examined the expression pattern of BHLHE40 in the developing chicken eye primordia by in situ hybridization. Secondly, BHLHE40 overexpression was performed with in ovo electroporation and its effects on optic cup morphology and expression of NR and RPE marker genes were examined. Thirdly, we examined the expression pattern of BHLHE40 in LHX1-overexpressed optic cup. BHLHE40 expression emerged in a subset of cells of the OV at Hamburger and Hamilton stage 14 and became confined to the outer layer of the OV and the ciliary marginal zone of the retina by stage 17. BHLHE40 overexpression in the prospective NR resulted in ectopic induction of OTX2 and repression of VSX2. Conversely, BHLHE40 was repressed in the second NR after LHX1 overexpression. These results suggest that emergence of BHLHE40 expression in the OV is involved in initial RPE specification and that BHLHE40 plays a role in separation of the early OV domains by maintaining OTX2 expression and antagonizing an NR developmental program.

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  • Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice. 査読 国際誌

    Ruizhi Xue, Wenfeng Lin, Hirofumi Fujita, Jingkai Sun, Rie Kinoshita, Kazuhiko Ochiai, Junichiro Futami, Masami Watanabe, Hideyo Ohuchi, Masakiyo Sakaguchi, Zhengyan Tang, Peng Huang, Yasutomo Nasu, Hiromi Kumon

    Genes   13 ( 2 )   2022年1月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    The role of Dickkopf-3 (Dkk3)/REIC (The Reduced Expression in Immortalized Cells), a Wnt-signaling inhibitor, in male reproductive physiology remains unknown thus far. To explore the functional details of Dkk3/REIC in the male reproductive process, we studied the Dkk3/REIC knock-out (KO) mouse model. By examining testicular sections and investigating the sperm characteristics (count, vitality and motility) and ultrastructure, we compared the reproductive features between Dkk3/REIC-KO and wild-type (WT) male mice. To further explore the underlying molecular mechanism, we performed RNA sequencing (RNA-seq) analysis of testicular tissues. Our results showed that spermiation failure existed in seminiferous tubules of Dkk3/REIC-KO mice, and sperm from Dkk3/REIC-KO mice exhibited inferior motility (44.09 ± 8.12% vs. 23.26 ± 10.02%, p < 0.01). The Ultrastructure examination revealed defects in the sperm fibrous sheath of KO mice. Although the average count of Dkk3/REIC-KO epididymal sperm was less than that of the wild-types (9.30 ± 0.69 vs. 8.27 ± 0.87, ×106), neither the gap (p > 0.05) nor the difference in the sperm vitality rate (72.83 ± 1.55% vs. 72.50 ± 0.71%, p > 0.05) were statistically significant. The RNA-seq and GO (Gene Oncology) enrichment results indicated that the differential genes were significantly enriched in the GO terms of cytoskeleton function, cAMP signaling and calcium ion binding. Collectively, our research demonstrates that Dkk3/REIC is involved in the process of spermiation, fibrous sheath integrity maintenance and sperm motility of mice.

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  • Amino acid residue at position 188 determines the UV-sensitive bistable property of vertebrate non-visual opsin Opn5. 査読 国際誌

    Chihiro Fujiyabu, Keita Sato, Yukimi Nishio, Yasushi Imamoto, Hideyo Ohuchi, Yoshinori Shichida, Takahiro Yamashita

    Communications biology   5 ( 1 )   63 - 63   2022年1月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    Opsins are G protein-coupled receptors specialized for photoreception in animals. Opn5 is categorized in an independent opsin group and functions for various non-visual photoreceptions. Among vertebrate Opn5 subgroups (Opn5m, Opn5L1 and Opn5L2), Opn5m and Opn5L2 bind 11-cis retinal to form a UV-sensitive resting state, which is inter-convertible with the all-trans retinal bound active state by photoreception. Thus, these opsins are characterized as bistable opsins. To assess the molecular basis of the UV-sensitive bistable property, we introduced comprehensive mutations at Thr188, which is well conserved among these opsins. The mutations in Opn5m drastically hampered 11-cis retinal incorporation and the bistable photoreaction. Moreover, T188C mutant Opn5m exclusively bound all-trans retinal and thermally self-regenerated to the original form after photoreception, which is similar to the photocyclic property of Opn5L1 bearing Cys188. Therefore, the residue at position 188 underlies the UV-sensitive bistable property of Opn5m and contributes to the diversification of vertebrate Opn5 subgroups.

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  • Toll signalling promotes blastema cell proliferation during cricket leg regeneration via insect macrophages 査読

    Tetsuya Bando, Misa Okumura, Yuki Bando, Marou Hagiwara, Yoshimasa Hamada, Yoshiyasu Ishimaru, Taro Mito, Eri Kawaguchi, Takeshi Inoue, Kiyokazu Agata, Sumihare Noji, Hideyo Ohuchi

    Development   149 ( 8 )   2021年11月

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    担当区分：最終著者,　責任著者   掲載種別：研究論文（学術雑誌）   出版者・発行元：The Company of Biologists  

    Hemimetabolous insects, such as the two-spotted cricket Gryllus bimaculatus, can recover lost tissues in contrast to the limited regenerative abilities in human tissues. Following cricket leg amputation, the wound surface is covered by the wound epidermis, and plasmatocytes, which are insect macrophages, accumulate in the wound region. Here, we studied the function of Toll-related molecules identified by comparative RNA-seq during leg regeneration. Among 11 Toll genes in the Gryllus genome, expression of Gb'Toll2-1, Gb'Toll2-2, and Gb'Toll2-5 was upregulated during regeneration. RNA interference (RNAi) of Gb'Toll, Gb'Toll2-1, Gb'Toll2-2, Gb'Toll2-3, or Gb'Toll2-4 produced regeneration defects in more than 50% of crickets. RNAi of Gb'Toll2-2 decreased the ratios of S and M phase cells, expression of JAK/STAT signalling genes, and accumulation of plasmatocytes in the blastema. Depletion of plasmatocytes in crickets using clodronate also produced regeneration defects, along with reduced proliferating cells in the regenerating legs. Plasmatocyte depletion also downregulated the expression of Toll and JAK/STAT signalling genes in the regenerating legs. These results suggest that Spz-Toll-related signalling in plasmatocytes promotes leg regeneration through blastema cell proliferation by regulating the Upd-JAK/STAT signalling pathway.

    DOI： 10.1242/dev.199916

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  • UCP1 expression in the mouse adrenal gland is not upregulated by thermogenic conditions. 査読 国際誌

    Hirofumi Fujita, Munenori Habuta, Takako Hattori, Satoshi Kubota, Hiromi Kumon, Hideyo Ohuchi

    Biochemical and biophysical research communications   566   184 - 189   2021年8月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    The uncoupling protein 1 (UCP1) gene is known to be highly expressed in brown adipose tissue (BAT) that functions in thermogenesis. It has been shown that UCP1 mRNA is localized to the mouse adrenal gland, but its significance remains elusive. To explore how UCP1 expression in the adrenal gland is regulated, we generated a reporter knock-in mouse in which the GFP gene was inserted into the UCP1 locus using CRISPR-Cas9 system. Firstly, we confirmed by Western blot analysis UCP1-driven GFP protein expression in interscapular BAT of the knock-in mice kept at 4 °C. Immunohistochemistry showed that GFP protein was detected in the adrenal gland of the knock-in mice. More intense GFP expression was observed in the adrenal medulla than in the cortex of the reporter mice irrespectively of cold exposure. Immunohistochemistry using anti-UCP1 antibody, as well as Western blot analysis verified UCP1 protein expression in the wild-type adrenal medulla. These results suggest that the mouse adrenal gland is a novel organ expressing UCP1 protein and its expression is not upregulated by cold exposure.

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  • Dkk3/REIC, an N-glycosylated Protein, Is a Physiological Endoplasmic Reticulum Stress Inducer in the Mouse Adrenal Gland. 査読

    Hirofumi Fujita, Tetsuya Bando, Seiichi Oyadomari, Kazuhiko Ochiai, Masami Watanabe, Hiromi Kumon, Hideyo Ohuchi

    Acta medica Okayama   74 ( 3 )   199 - 208   2020年6月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    Dickkopf 3 (Dkk3) is a secreted protein belonging to the Dkk family and encoded by the orthologous gene of REIC. Dkk3/REIC is expressed by mouse and human adrenal glands, but the understanding of its roles in this organ is still limited. To determine the functions of Dkk3 in the mouse adrenal gland, we first identified that the mouse Dkk3 protein is N-glycosylated in the adrenal gland as well as in the brain. We performed proteome analysis on adrenal glands from Dkk3-null mice, in which exons 5 and 6 of the Dkk3 gene are deleted. Twodimensional polyacrylamide gel electrophoresis of adrenal proteins from wild-type and Dkk3-null mice revealed 5 protein spots whose intensities were altered between the 2 genotypes. Mass spectrometry analysis of these spots identified binding immunoglobulin protein (BiP), an endoplasmic reticulum (ER) chaperone. To determine whether mouse Dkk3 is involved in the unfolded protein response (UPR), we carried out a reporter assay using ER-stress responsive elements. Forced expression of Dkk3 resulted in the induction of distinct levels of reporter expression, showing the UPR initiated by the ER membrane proteins of activating transcription factor 6 (ATF6) and inositol-requring enzyme 1 (IRE1). Thus, it is possible that Dkk3 is a physiological ER stressor in the mouse adrenal gland.

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  • Dickkopf3 (Dkk3) is required for maintaining the integrity of secretory vesicles in the mouse adrenal medulla. 査読 国際誌

    Habuta M, Fujita H, Sato K, Bando T, Inoue J, Kondo Y, Miyaishi S, Kumon H, Ohuchi H

    Cell and tissue research   379 ( 1 )   157 - 167   2019年10月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1007/s00441-019-03113-8

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  • Evolutionary history of teleost intron-containing and intron-less rhodopsin genes. 査読 国際誌

    Fujiyabu C, Sato K, Utari NML, Ohuchi H, Shichida Y, Yamashita T

    Scientific reports   9 ( 1 )   10653 - 10653   2019年7月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/s41598-019-47028-4

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  • Publisher Correction: Fgf10 is essential for limb and lung formation. 国際誌

    Keisuke Sekine, Hideyo Ohuchi, Masanori Fujiwara, Masahiro Yamasaki, Tatsuya Yoshizawa, Takashi Sato, Naoko Yagishita, Daisuke Matsui, Yoshihiko Koga, Nobuyuki Itoh, Shigeaki Kato

    Nature genetics   51 ( 5 )   921 - 921   2019年5月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    In the version of the paper initially published, Fig. 5a was inadvertently duplicated and presented as both Fig. 5a and 5f, and the correct image for Fig. 5f was omitted. Images of the original and corrected figure panels are shown in the correction notice.

    DOI： 10.1038/s41588-019-0396-9

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  • Glutathione accelerates osteoclast differentiation and inflammatory bone destruction. 査読 国際誌

    Fujita H, Ochi M, Ono M, Aoyama E, Ogino T, Kondo Y, Ohuchi H

    Free radical research   53 ( 2 )   226 - 236   2019年2月

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    担当区分：最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1080/10715762.2018.1563782

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  • Bone morphogenetic protein signaling in distal patterning and intercalation during leg regeneration of the cricket, Gryllus bimaculatus. 査読

    Ishimaru Y, Bando T, Ohuchi H, Noji S, Mito T

    Development, growth & differentiation   60 ( 6 )   377 - 386   2018年8月

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  • Pinopsin evolved as the ancestral dim-light visual opsin in vertebrates. 査読 国際誌

    Sato K, Yamashita T, Kojima K, Sakai K, Matsutani Y, Yanagawa M, Yamano Y, Wada A, Iwabe N, Ohuchi H, Shichida Y

    Communications biology   1   156 - 156   2018年

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/s42003-018-0164-x

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  • Expression analysis of Dickkopf-related protein 3 (Dkk3) suggests its pleiotropic roles for a secretory glycoprotein in adult mouse 査読

    Junji Inoue, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Hiromi Kumon, Hideyo Ohuchi

    JOURNAL OF MOLECULAR HISTOLOGY   48 ( 1 )   29 - 39   2017年2月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1007/s10735-016-9703-2

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  • Eye development and photoreception of a hemimetabolous insect, gryllus bimaculatus 招待 査読

    Hideyo Ohuchi, Tetsuya Bando, Taro Mito, Sumihare Noji

    The Cricket as a Model Organism: Development, Regeneration, and Behavior   49 - 62   2017年1月

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    担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：論文集(書籍)内論文   出版者・発行元：Springer Japan  

    DOI： 10.1007/978-4-431-56478-2_4

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  • 抗酸化物質グルタチオンは炎症性骨破壊を促進する

    藤田 洋史, 大野 充昭, 青山 絵理子, 大内 淑代

    日本骨代謝学会学術集会プログラム抄録集   34回   206 - 206   2016年7月

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    記述言語：日本語   出版者・発行元：(一社)日本骨代謝学会  

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  • TGF-β signaling in insects regulates metamorphosis via juvenile hormone biosynthesis. 査読

    Ishimaru Y, Tomonari S, Matsuoka Y, Watanabe T, Miyawaki K, Bando T, Tomioka K, Ohuchi H, Noji S, Mito T

    Proceedings of the National Academy of Sciences of the United States of America   113 ( 20 )   5634 - 5639   2016年5月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1073/pnas.1600612113

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  • Two UV-Sensitive Photoreceptor Proteins, Opn5m and Opn5m2 in Ray-Finned Fish with Distinct Molecular Properties and Broad Distribution in the Retina and Brain 査読

    Keita Sato, Takahiro Yamashita, Yoshihiro Haruki, Hideyo Ohuchi, Masato Kinoshita, Yoshinori Shichida

    PLOS ONE   11 ( 5 )   e0155339   2016年5月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1371/journal.pone.0155339

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  • Autopsy Case of Bilateral Optic Nerve Aplasia with Microphthalmia: Neural Retina Formation Is Required for the Coordinated Development of Ocular Tissues 査読

    Hideyo Ohuchi, Kaori Taniguchi, Satoru Miyaishi, Hitomi Kono, Hirofumi Fujita, Tetsuya Bando, Chiharu Fuchizawa, Yuko Ohtani, Osamu Ohtani

    ACTA MEDICA OKAYAMA   70 ( 2 )   131 - 137   2016年4月

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    担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • Phytoestrogen Suppresses Efflux of the Diagnostic Marker Protoporphyrin IX in Lung Carcinoma 査読

    Hirofumi Fujita, Keisuke Nagakawa, Hirotsugu Kobuchi, Tetsuya Ogino, Yoichi Kondo, Keiji Inoue, Taro Shuin, Toshihiko Utsumi, Kozo Utsumi, Junzo Sasaki, Hideyo Ohuchi

    CANCER RESEARCH   76 ( 7 )   1837 - 1846   2016年4月

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    担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1158/0008-5472.CAN-15-1484

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  • Enhancer of zeste plays an important role in photoperiodic modulation of locomotor rhythm in the cricket, Gryllus bimaculatus. 査読 国際誌

    Yoshimasa Hamada, Atsushi Tokuoka, Tetsuya Bando, Hideyo Ohuchi, Kenji Tomioka

    Zoological letters   2   5 - 5   2016年

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    INTRODUCTION: Insects show daily behavioral rhythms controlled by an endogenous oscillator, the circadian clock. The rhythm synchronizes to daily light-dark cycles (LD) and changes waveform in association with seasonal change in photoperiod. RESULTS: To explore the molecular basis of the photoperiod-dependent changes in circadian locomotor rhythm, we investigated the role of a chromatin modifier, Enhancer of zeste (Gb'E(z)), in the cricket, Gryllus bimaculatus. Under a 12 h:12 h LD (LD 12:12), Gb'E(z) was constitutively expressed in the optic lobe, the site of the biological clock; active phase (α) and rest phase (ρ) were approximately 12 h in duration, and α/ρ ratio was approximately 1.0. When transferred to LD 20:4, the α/ρ ratio decreased significantly, and the Gb'E(z) expression level was significantly reduced at 6 h and 10 h after light-on, as was reflected in the reduced level of trimethylation of histone H3 lysine 27. This change was associated with change in clock gene expression profiles. The photoperiod-dependent changes in α/ρ ratio and clock gene expression profiles were prevented by knocking down Gb'E(z) by RNAi. CONCLUSIONS: These results suggest that histone modification by Gb'E(z) is involved in photoperiodic modulation of the G. bimaculatus circadian rhythm.

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  • マウス頭蓋冠におけるリポポリサッカリドによる骨破壊とグルタチオンの効果

    藤田 洋史, 大野 充昭, 青山 絵理子, 荻野 哲也, 近藤 洋一, 大内 淑代

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集   88回・38回   [1LBA049] - [1LBA049]   2015年12月

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    記述言語：日本語   出版者・発行元：(公社)日本生化学会  

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  • Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus 査読

    Yoshimasa Hamada, Tetsuya Bando, Taro Nakamura, Yoshiyasu Ishimaru, Taro Mito, Sumihare Noji, Kenji Tomioka, Hideyo Ohuchi

    DEVELOPMENT   142 ( 17 )   2916 - +   2015年9月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1242/dev.122598

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  • Involvement of dachshund and Distal-less in distal pattern formation of the cricket leg during regeneration 査読

    Yoshiyasu Ishimaru, Taro Nakamura, Tetsuya Bando, Yuji Matsuoka, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    SCIENTIFIC REPORTS   5   8387   2015年2月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/srep08387

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  • An extended steepness model for leg-size determination based on Dachsous/Fat trans- dimer system 査読

    Hiroshi Yoshida, Tetsuya Bando, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    SCIENTIFIC REPORTS   4   4335   2014年3月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/srep04335

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  • The expression of LIM-homeobox genes, Lhx1 and Lhx5, in the forebrain is essential for neural retina differentiation 査読

    Junji Inoue, Yuuki Ueda, Tetsuya Bando, Taro Mito, Sumihare Noji, Hideyo Ohuchi

    DEVELOPMENT GROWTH & DIFFERENTIATION   55 ( 7 )   668 - 675   2013年9月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • Improvement of the Efficacy of 5-aminolevulinic Acid-mediated Photodynamic Treatment in Human Oral Squamous Cell Carcinoma HSC-4 査読

    Masanao Yamamoto, Hirofumi Fujita, Naoki Katase, Keiji Inoue, Hitoshi Nagatsuka, Kozo Utsumi, Junzo Sasaki, Hideyo Ohuchi

    ACTA MEDICA OKAYAMA   67 ( 3 )   153 - 164   2013年6月

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    担当区分：最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • Analysis of RNA-Seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus 査読

    Tetsuya Bando, Yoshiyasu Ishimaru, Takuro Kida, Yoshimasa Hamada, Yuji Matsuoka, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    DEVELOPMENT   140 ( 5 )   959 - 964   2013年3月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1242/dev.084590

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  • Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken 査読

    Takumi Kawaue, Mayumi Okamoto, Akane Matsuyo, Junji Inoue, Yuhki Ueda, Sayuri Tomonari, Sumihare Noji, Hideyo Ohuchi

    BIOLOGY OPEN   1 ( 11 )   1083 - 1093   2012年11月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1242/bio.20121396

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  • Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases 査読

    Takahito Watanabe, Hiroshi Ochiai, Tetsushi Sakuma, Hadley W. Horch, Naoya Hamaguchi, Taro Nakamura, Tetsuya Bando, Hideyo Ohuchi, Takashi Yamamoto, Sumihare Noji, Taro Mito

    NATURE COMMUNICATIONS   3   1017   2012年8月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/ncomms2020

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  • Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus 査読

    Akira Takagi, Kazuki Kurita, Taiki Terasawa, Taro Nakamura, Tetsuya Bando, Yoshiyuki Moriyama, Taro Mito, Sumihare Noji, Hideyo Ohuchi

    DEVELOPMENT GROWTH & DIFFERENTIATION   54 ( 2 )   227 - 240   2012年2月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1111/j.1440-169X.2011.01325.x

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  • Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an Agrobacterium-mediated RNAi technique 査読

    Katsuyuki Miyawaki, Sachi Fukuoka, Yasuko Kadomura, Hirokazu Hamaoka, Taro Mito, Hideyo Ohuchi, Wilfried Schwab, Sumihare Noji

    PLANT BIOTECHNOLOGY   29 ( 3 )   271 - 277   2012年

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.5511/plantbiotechnology.12.0406a

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  • Expression of myelin genes in the developing chick retina 査読

    Hitosh Gotoh, Takayuki Ueda, Aoi Uno, Hideyo Ohuchi, Kazuhiro Ikenaka, Katsuhiko Ono

    GENE EXPRESSION PATTERNS   11 ( 8 )   471 - 475   2011年12月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.gep.2011.08.002

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  • Vertebrate Ancient-Long Opsin Has Molecular Properties Intermediate between Those of Vertebrate and Invertebrate Visual Pigments 査読

    Keita Sato, Takahiro Yamashita, Hideyo Ohuchi, Yoshinori Shichida

    BIOCHEMISTRY   50 ( 48 )   10484 - 10490   2011年12月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1021/bi201212z

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  • Cricket body size is altered by systemic RNAi against insulin signaling components and epidermal growth factor receptor 査読

    Noha Dabour, Tetsuya Bando, Taro Nakamura, Katsuyuki Miyawaki, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   53 ( 7 )   857 - 869   2011年9月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1111/j.1440-169X.2011.01291.x

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  • Ancestral functions of Delta/Notch signaling in the formation of body and leg segments in the cricket Gryllus bimaculatus 査読

    Taro Mito, Yohei Shinmyo, Kazuki Kurita, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT   138 ( 17 )   3823 - 3833   2011年9月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1242/dev.060681

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  • Lowfat, a Mammalian Lix1 Homologue, Regulates Leg Size and Growth Under the Dachsous/Fat Signaling Pathway During Tissue Regeneration 査読

    Tetsuya Bando, Yoshimasa Hamada, Kazuki Kurita, Taro Nakamura, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENTAL DYNAMICS   240 ( 6 )   1440 - 1453   2011年6月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/dvdy.22647

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  • Regulation of Leg Size and Shape: Involvement of the Dachsous-Fat Signaling Pathway 査読

    Tetsuya Bando, Taro Mito, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENTAL DYNAMICS   240 ( 5 )   1028 - 1041   2011年5月

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  • Autotaxin Is Required for the Cranial Neural Tube Closure and Establishment of the Midbrain-Hindbrain Boundary During Mouse Development 査読

    Seiichi Koike, Yoshifumi Yutoh, Kazuko Keino-Masu, Sumihare Noji, Masayuki Masu, Hideyo Ohuchi

    DEVELOPMENTAL DYNAMICS   240 ( 2 )   413 - 421   2011年2月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/dvdy.22543

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  • Autotaxin is required for the cranial neural tube closure and establishment of the midbrain-hindbrain boundary during mouse development 査読

    Seiichi Koike, Yoshifumi Yutoh, Kazuko Keino-Masu, Sumihare Noji, Masayuki Masu, Hideyo Ohuchi

    Developmental Dynamics   240 ( 2 )   413 - 421   2011年2月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/dvdy.22543

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  • Atelocollagen-mediated systemic administration of myostatin-targeting siRNA improves muscular atrophy in caveolin-3-deficient mice 査読

    Emi Kawakami, Nao Kinouchi, Taro Adachi, Yutaka Ohsawa, Naozumi Ishimaru, Hideyo Ohuchi, Yoshihide Sunada, Yoshio Hayashi, Eiji Tanaka, Sumihare Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   53 ( 1 )   48 - 54   2011年1月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1111/j.1440-169X.2010.01221.x

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  • Delivery of small interfering RNA with a synthetic collagen poly(Pro-Hyp-Gly) for gene silencing in vitro and in vivo 査読

    Taro Adachi, Emi Kawakami, Naozumi Ishimaru, Takahiro Ochiya, Yoshio Hayashi, Hideyo Ohuchi, Masao Tanihara, Eiji Tanaka, Sumihare Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   52 ( 8 )   693 - 699   2010年10月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1111/j.1440-169X.2010.01206.x

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  • Autotaxin Controls Caudal Diencephalon-Mesencephalon Development in the Chick 査読

    Hideyo Ohuchi, Hitomi Fukui, Akane Matsuyo, Sayuri Tomonari, Masayuki Tanaka, Hiroyuki Arai, Sumihare Noji, Junken Aoki

    DEVELOPMENTAL DYNAMICS   239 ( 10 )   2647 - 2658   2010年10月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/dvdy.22403

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  • Imaging of Transgenic Cricket Embryos Reveals Cell Movements Consistent with a Syncytial Patterning Mechanism 査読

    Taro Nakamura, Masato Yoshizaki, Shotaro Ogawa, Haruko Okamoto, Yohei Shinmyo, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    CURRENT BIOLOGY   20 ( 18 )   1641 - 1647   2010年9月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.cub.2010.07.044

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  • Loss-of-Function Analyses of the Fragile X-Related and Dopamine Receptor Genes by RNA Interference in the Cricket Gryllus bimaculatus 査読

    Aska Hamada, Katsuyuki Miyawaki, Eri Honda-sumi, Kenji Tomioka, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENTAL DYNAMICS   238 ( 8 )   2025 - 2033   2009年8月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration 査読

    Tetsuya Bando, Taro Mito, Yuko Maeda, Taro Nakamura, Fumiaki Ito, Takahito Watanabe, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT   136 ( 13 )   2235 - 2245   2009年7月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1242/dev.035204

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  • Subtype-specific expression of Fgf19 during horizontal cell development of the chicken retina 査読

    Mayumi Okamoto, Takaaki Bito, Sumihare Noji, Hideyo Ohuchi

    GENE EXPRESSION PATTERNS   9 ( 5 )   306 - 313   2009年6月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.gep.2009.02.007

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  • Expression Patterns of the Lysophospholipid Receptor Genes During Mouse Early Development 査読

    Hideyo Ohuchi, Aska Hamada, Hironao Matsuda, Akira Takagi, Masayuki Tanaka, Junken Aoki, Hiroyuki Arai, Sumihare Noji

    DEVELOPMENTAL DYNAMICS   237 ( 11 )   3280 - 3294   2008年11月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/dvdy.21736

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  • Atelocollagen-mediated local and systemic applications of myostatin-targeting siRNA increase skeletal muscle mass.

    Kinouchi N, Ohsawa Y, Ishimaru N, Ohuchi H, Sunada Y, Hayashi Y, Tanimoto Y, Moriyama K, Noji S

    Gene Therapy   15 ( 15 )   1126 - 1130   2008年8月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    RNA interference (RNAi) offers a novel therapeutic strategy based on the highly specific and efficient silencing of a target gene. Since it relies on small interfering RNAs (siRNAs), a major issue is the delivery of therapeutically active siRNAs into the target tissue/target cells in vivo. For safety reasons, strategies based on vector delivery may be of only limited clinical use. The more desirable approach is to directly apply active siRNAs in vivo. Here, we report the effectiveness of in vivo siRNA delivery into skeletal muscles of normal or diseased mice through nanoparticle formation of chemically unmodified siRNAs with atelocollagen (ATCOL). ATCOL-mediated local application of siRNA targeting myostatin, a negative regulator of skeletal muscle growth, in mouse skeletal muscles or intravenously, caused a marked increase in the muscle mass within a few weeks after application. These results imply that ATCOL-mediated application of siRNAs is a powerful tool for future therapeutic use for diseases including muscular atrophy.

    DOI： 10.1038/gt.2008.24

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  • Dynamic expression patterns of vasa during embryogenesis in the cricket Gryllus bimaculatus 査読

    Taro Mito, Taro Nakamura, Isao Sarashina, Chun-che Chang, Shotaro Ogawa, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT GENES AND EVOLUTION   218 ( 7 )   381 - 387   2008年7月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1007/s00427-008-0226-z

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  • EGFR signaling is required for re-establishing the proximodistal axis during distal leg regeneration in the cricket Gryllus bimaculatus nymph 査読

    Taro Nakamura, Taro Mito, Katsuyuki Miyawaki, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENTAL BIOLOGY   319 ( 1 )   46 - 55   2008年7月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.ydbio.2008.04.002

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  • Introduction of silencing-inducing transgene against Fgf19 does not affect expression of Tbx5 and beta 3-tubulin in the developing chicken retina 査読

    Mayumi Okamoto, Sayuri Tomonari, Yuki Naito, Kaoru Saigo, Sumihare Noji, Kumiko Ui-Tei, Hideyo Ohuchi

    DEVELOPMENT GROWTH & DIFFERENTIATION   50 ( 3 )   159 - 168   2008年4月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1111/j.1440-169x.2008.00996.x

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  • Divergent and conserved roles of extradenticle in body segmentation and appendage formation, respectively, in the cricket Gryllus bimaculatus 査読

    Taro Mito, Monica Ronco, Tomohiro Uda, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENTAL BIOLOGY   313 ( 1 )   67 - 79   2008年1月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.ydbio.2007.09.060

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  • Film tomography as a tool for three-dimensional image construction and gene expression studies 査読

    Masayuki Mitsumori, Taro Adachi, Kazuho Takayanagi, Taro Mito, Hideyo Ohuchi, Shouichi Kimura, Mitsunori Kokubo, Toshiro Higuchi, Sumihare Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   49 ( 7 )   583 - 589   2007年9月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • Expression pattern of the melanopsin-like (cOpn4m) and VA opsin-like genes in the developing chicken retina and neural tissues 査読

    Sayuri Tomonari, Akira Takagi, Sumittare Noji, Hideyo Ohuchi

    GENE EXPRESSION PATTERNS   7 ( 7 )   746 - 753   2007年8月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.modgep.2007.06.001

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  • Diversified expression patterns of autotaxin, a gene for phospholipid-generating enzyme during mouse and chicken development (vol 236, pg 1134, 2007) 査読

    Ohuchi Hideyo, Hayashibara Yasunori, Matsuda Hironao, Onoi Motoyoshi, Mitsumori Masayuki, Tanaka Masayuki, Aoki Junken, Arai Hiroyuki, Noji Sumihare

    DEVELOPMENTAL DYNAMICS   236 ( 5 )   1376   2007年5月

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  • Diversified expression patterns of autotaxin, a gene for phospholipid-generating enzyme during mouse and chicken development 査読

    Hideyo Ohuchi, Yasunori Hayashibaral, Hironao Matsuda, Motoyoshi Onoi, Masayuki Mitsumori, Masayuki Tanaka, Junken Aoki, Hiroyuki Arai, Sumihare Noji

    DEVELOPMENTAL DYNAMICS   236 ( 4 )   1134 - 1143   2007年4月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/dvdy.21119

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  • even-skipped has gap-like, pair-rule-like, and segmental functions in the cricket Gryllus bimaculatus, a basal, intermediate germ insect (Orthoptera) 査読

    Taro Mito, Chiharu Kobayashi, Isao Sarashina, Hongjie Zhang, Wakako Shinahara, Katsuyuki Miyawaki, Yohei Shinmyo, Hideyo Ohuchi, Sumihare Noji

    Developmental Biology   303 ( 1 )   202 - 213   2007年3月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Academic Press Inc.  

    DOI： 10.1016/j.ydbio.2006.11.003

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  • Involvement of canonical Wnt/Wingless signaling in the determination of the positional values within the leg segment of the cricket Gryllus bimaculatus 査読

    Taro Nakamura, Taro Mito, Yoshihisa Tanaka, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   49 ( 2 )   79 - 88   2007年2月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1111/j.1440-169x.2007.00915.x

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  • Characterization of follistatin-related gene as a negative regulatory factor for activin family members during mouse heart development 査読

    Yuka Takehara-Kasamatsu, Kunihiro Tsuchida, Masashi Nakatani, Tatsuya Murakami, Akira Kurisaki, Osamu Hashimoto, Hideyo Ohuchi, Hitomi Kurose, Kazuhiro Mori, Shoji Kagami, Sumihare Noji, Hiromu Sugino

    Journal of Medical Investigation   54 ( 3-4 )   276 - 288   2007年

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    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：3-4  

    DOI： 10.2152/jmi.54.276

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  • Brachyenteron is necessary for morphogenesis of theposterior gut but not for anteroposterior axial elongationfrom the posterior growth zone in the intermediate-germbandcricket Gryllus bimaculatus 査読

    Yohei Shinmyo, Taro Mito, Tomohiro Uda, Taro Nakamura, Katsuyuki Miyawaki, Hideyo Ohuchi, Sumihare Noji

    Development   133 ( 22 )   4539 - 4547   2006年11月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1242/dev.02646

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  • Krüppel acts as a gap gene regulating expression of hunchback and even-skipped in the intermediate germ cricket Gryllus bimaculatus. 査読

    Mito T, Okamoto H, Shinahara W, Shinmyo Y, Miyawaki K, Ohuchi H, Noji S

    Developmental biology   294 ( 2 )   471 - 481   2006年6月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.ydbio.2005.12.057

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  • Exogenous FGF10 can rescue an eye-open at birth phenotype of Fgf10-null mice by activating activin and TGF alpha-EGFR signaling 査読

    H Tao, K Ono, H Kurose, S Noji, H Ohuchi

    DEVELOPMENT GROWTH & DIFFERENTIATION   48 ( 5 )   339 - 346   2006年6月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1111/j.1440-169x.2006.00869.x

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  • Cessation of Fgf10 signaling, resulting in a defective dental epithelial stem cell compartment, leads to the transition from crown to root formation 査読

    T Yokohama-Tamaki, H Ohshima, N Fujiwara, Y Takada, Y Ichimori, S Wakisaka, H Ohuchi, H Harada

    DEVELOPMENT   133 ( 7 )   1359 - 1366   2006年4月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1242/dev.02307

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  • A non-canonical photopigment, melanopsin, is expressed in the differentiating ganglion, horizontal, and bipolar cells of the chicken retina 査読

    S Tomonari, A Takagi, S Akamatsu, S Noji, H Ohuchi

    DEVELOPMENTAL DYNAMICS   234 ( 3 )   783 - 790   2005年11月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • Identification of cis-element regulating expression of the mouse Fgf10 gene during inner ear development 査読

    H Ohuchi, A Yasue, K Ono, S Sasaoka, S Tomonari, A Takagi, M Itakura, K Moriyama, S Noji, T Nohno

    DEVELOPMENTAL DYNAMICS   233 ( 1 )   177 - 187   2005年5月

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    担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • Non-canonical functions of hunchback in segment patterning of the intermediate germ cricket Gryllus bimaculatus 査読

    Taro Mito, Isao Sarashina, Hongjie Zhang, Akihiro Iwahashi, Haruko Okamoto, Katsuyuki Miyawaki, Yohei Shinmyo, Hideyo Ohuchi, Sumihare Noji

    Development   132 ( 9 )   2069 - 2079   2005年5月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1242/dev.01784

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  • FGF19-FGFR4 signaling elaborates lens induction with the FGF8-L-Maf cascade in the chick embryo 査読

    H Kurose, M Okamoto, M Shimizu, T Bito, C Marcelle, S Noji, H Ohuchi

    DEVELOPMENT GROWTH & DIFFERENTIATION   47 ( 4 )   213 - 223   2005年5月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1111/j.1440-169X.2005.00795.x

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  • [Evolution of insect developmental systems: from the cricket mode to the fly mode]. 査読

    Mito T, Sarashina I, Ohuchi H, Noji S

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   50   750 - 755   2005年5月

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    出版者・発行元：6 Suppl  

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  • Expression patterns of the homeotic genes Scr, Antp, Ubx, and abd-A during embryogenesis of the cricket Gryllus bimaculatus 査読

    Hongjie Zhang, Yohei Shinmyo, Taro Mito, Katsuyuki Miyawaki, Isao Sarashina, Hideyo Ohuchi, Sumihare Noji

    Gene Expression Patterns   5 ( 4 )   491 - 502   2005年4月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.modgep.2004.12.006

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  • Location of micropyles and early embryonic development of the two-spotted cricket Gryllus bimaculatus (Insecta, Orthoptera) 査読

    Isao Sarashina, Taro Mito, Michiko Saito, Hiroyuki Uneme, Katsuyuki Miyawaki, Yohei Shinmyo, Hideyo Ohuchi, Sumihare Noji

    Development Growth and Differentiation   47 ( 2 )   99 - 108   2005年2月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1111/j.1440-169x.2005.00786.x

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  • caudal is required for gnathal and thoracic patterning and for posterior elongation in the intermediate-germband cricket Gryllus bimaculatus 査読

    Yohei Shinmyo, Taro Mito, Takashi Matsushita, Isao Sarashina, Katsuyuki Miyawaki, Hideyo Ohuchi, Sumihare Noji

    Mechanisms of Development   122 ( 2 )   231 - 239   2005年2月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.mod.2004.10.001

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  • Expression of Fibroblast growth factor 19 (Fgf19) during chicken embryogenesis and eye development, compared with Fgf15 expression in the mouse 査読

    H Kurose, T Bito, T Adachi, M Shimizu, S Noji, H Ohuchi

    GENE EXPRESSION PATTERNS   4 ( 6 )   687 - 693   2004年10月

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.modgep.2004.04.005

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  • Expression patterns of dachshund during head development of Gryllus bimaculatus (cricket). 査読 国際誌

    Inoue Y, Miyawaki K, Terasawa T, Matsushima K, Shinmyo Y, Niwa N, Mito T, Ohuchi H, Noji S

    Gene expression patterns : GEP   4 ( 6 )   725 - 731   2004年10月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.modgep.2004.03.010

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  • piggyBac-mediated somatic transformation of the two-spotted cricket, Gryllus bimaculatus 査読

    Yohei Shinmyo, Taro Mito, Takashi Matsushita, Isao Sarashina, Katsuyuki Miyawaki, Hideyo Ohuchi, Sumihare Noji

    Development Growth and Differentiation   46 ( 4 )   343 - 349   2004年8月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1111/j.1440-169x.2004.00751.x

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  • Involvement of Wingless/Armadillo signaling in the posterior sequential segmentation in the cricket, Gryllus bimaculatus (Orthoptera), as revealed by RNAi analysis 査読

    Katsuyuki Miyawaki, Taro Mito, Isao Sarashina, Hongjie Zhang, Yohei Shinmyo, Hideyo Ohuchi, Sumihare Noji

    Mechanisms of Development   121 ( 2 )   119 - 130   2004年2月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.mod.2004.01.002

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  • Hypotonic buffer induces meiosis and formation of anucleate cytoplasmic islands in the egg of the two-spotted cricket Gryllus bimaculatus 査読

    Isao Sarashina, Yohei Shinmyo, Ayumi Hirose, Katsuyuki Miyawaki, Taro Mito, Hideyo Ohuchi, Tetsuya Horio, Sumihare Noji

    Development Growth and Differentiation   45 ( 2 )   103 - 112   2003年4月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1034/j.1600-0854.2004.00679.x

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  • Fibroblast growth factor 10 is required for proper development of the mouse whiskers 査読

    H Ohuchi, H Tao, K Ohata, N Itoh, S Kato, S Noji, K Ono

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   302 ( 3 )   562 - 567   2003年3月

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    担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/S0006-291X(03)00183-9

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  • Extrachromosomal transposition of the transposable element Minos in embryos of the cricket Gryllus bimaculatus. 査読

    Zhang H, Shinmyo Y, Hirose A, Mito T, Inoue Y, Ohuchi H, Loukeris TG, Eggleston P, Noji S

    Development, growth & differentiation   44 ( 5 )   409 - 417   2002年10月

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    記述言語：英語   出版者・発行元：Japanese Society of Developmental Biologists  

    DOI： 10.1046/j.1440-169X.2002.00654.x

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  • Involvement of hedgehog, wingless, and dpp in the initiation of proximodistal axis formation during the regeneration of insect legs, a verification of the modified boundary model. 査読

    Mito T, Inoue Y, Kimura S, Miyawaki K, Niwa N, Shinmyo Y, Ohuchi H, Noji S

    Mechanisms of development   114 ( 1-2 )   27 - 35   2002年6月

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  • Correlation of expression patterns of homothorax, dachshund, and Distal-less with the proximodistal segmentation of the cricket leg bud. 査読 国際誌

    Inoue Y, Mito T, Miyawaki K, Matsushima K, Shinmyo Y, Heanue TA, Mardon G, Ohuchi H, Noji S

    Mechanisms of development   113 ( 2 )   141 - 148   2002年5月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/S0925-4773(02)00017-5

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  • Expression patterns of aristaless in developing appendages of Gryllus bimaculatus (cricket). 査読 国際誌

    Miyawaki K, Inoue Y, Mito T, Fujimoto T, Matsushima K, Shinmyo Y, Ohuchi H, Noji S

    Mechanisms of development   113 ( 2 )   181 - 184   2002年5月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/S0925-4773(02)00020-5

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  • FGF10 maintains stem cell compartment in developing mouse incisors. 査読 国際誌

    Harada H, Toyono T, Toyoshima K, Yamasaki M, Itoh N, Kato S, Sekine K, Ohuchi H

    Development (Cambridge, England)   129 ( 6 )   1533 - 1541   2002年3月

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  • FGF10 maintains stem cell population during mouse incisor development 査読

    Hidemitsu Harada, Takashi Toyono, Kuniaki Toyoshima, Hideyo Ohuchi

    Connective Tissue Research   43 ( 2-3 )   201 - 204   2002年

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  • Identification of cis-elements regulating expression of Fgf10 during limb development 査読

    H Sasaki, T Yamaoka, H Ohuchi, A Yasue, T Nohno, H Kawano, S Kato, M Itakura, M Nagayama, S Noji

    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY   46 ( 7 )   963 - 967   2002年

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  • A missense mutant myostatin causes hyperplasia without hypertrophy in the mouse muscle

    Masumi Nishi, Akihiro Yasue, Shinichirou Nishimatu, Tsutomu Nohno, Takashi Yamaoka, Mitsuo Itakura, Keiji Moriyama, Hideyo Ohuchi, Sumihare Noji

    Biochemical and Biophysical Research Communications   293 ( 1 )   247 - 251   2002年

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/S0006-291X(02)00209-7

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  • Expression patterns of hedgehog, wingless, and decapentaplegic during gut formation of Gryllus bimaculatus (cricket)

    Yoshiko Inoue, Nao Niwa, Taro Mito, Hideyo Ohuchi, Hidefumi Yoshioka, Sumihare Noji

    Mechanisms of Development   110   245 - 248   2001年12月

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  • Differential expression of two BMP antagonists, gremlin and Follistatin, during development of the chick feather bud

    Akihiro Ohyama, Akihiro Ohyama, Fumi Saito, Hideyo Ohuchi, Sumihare Noji

    Mechanisms of Development   100   331 - 333   2001年2月

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  • Cloning and expression of the chick p63 gene

    Akihiro Yasue, Hirotaka Tao, Tsutomu Nohno, Keiji Moriyama, Sumihare Noji, Hideyo Ohuchi

    Mechanisms of Development   100 ( 1 )   105 - 108   2001年

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    担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/S0925-4773(00)00504-9

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  • Correlation of diversity of leg morphology in Gryllus bimaculatus (cricket) with divergence in dpp expression pattern during leg development 査読

    N Niwa, Y Inoue, A Nozawa, M Saito, Y Misumi, H Ohuchi, H Yoshioka, S Noji

    DEVELOPMENT   127 ( 20 )   4373 - 4381   2000年10月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • Involvement of fibroblast growth factor (FGF)18-FGF8 signaling in specification of left-right asymmetry and brain and limb development of the chick embryo 査読

    Hideyo Ohuchi, Sachie Kimura, Miki Watamoto, Nobuyuki Itoh

    Mechanisms of Development   95 ( 1-2 )   55 - 66   2000年7月

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    担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/S0925-4773(00)00331-2

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  • Asymmetric expression of antivin/lefty1 in the early chick embryo. 査読

    Ishimaru Y, Yoshioka H, Tao H, Thisse B, Thisse C, Christopher WVE, Hamada H, Ohuchi H, Noji S

    Mechanisms of Development   90 ( 1 )   115 - 118   2000年

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  • Mouse Pitx2 deficiency leads to anomalies of the ventral body wall, heart, extra- and periocular mesoderm and right pulmonary isomerism 査読

    Kunio Kitamura, Hirohito Miura, Sachiko Miyagawa-Tomita, Masako Yanazawa, Yuko Katoh-Fukui, Rika Suzuki, Hideyo Ohuchi, Atuko Suehiro, Yoshiko Motegi, Yoko Nakahara, Shunzo Kondo, Minesuke Yokoyama

    Development   126 ( 24 )   5749 - 5758   1999年12月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • FGF10 can induce Fgf8 expression concomitantly with En1 and R-fng expression in chick limb ectoderm, independent of its dorsoventral specification 査読

    H Ohuchi, T Nakagawa, N Itoh, S Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   41 ( 6 )   665 - 673   1999年12月

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    担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • FGF7 and FGF10 directly induce the apical ectodermal ridge in chick embryos 査読

    Sayuri Yonei-Tamura, Tetsuya Endo, Hiroshi Yajima, Hideyo Ohuchi, Hiroyuki Ide, Koji Tamura

    Developmental Biology   211 ( 1 )   133 - 143   1999年7月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Academic Press Inc.  

    DOI： 10.1006/dbio.1999.9290

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  • Identification of chick rax/rx genes with overlapping patterns of expression during early eye and brain development 査読

    H Ohuchi, S Tomonari, H Itoh, T Mikawa, S Noji

    MECHANISMS OF DEVELOPMENT   85 ( 1-2 )   193 - 195   1999年7月

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    担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • FGF-10 is a growth factor for preadipocytes in white adipose tissue 査読

    Masahiro Yamasaki, Hisayo Emoto, Morichika Konishi, Tadahisa Mikami, Hideyo Ohuchi, Kazuwa Nakao, Nobuyuki Itoh

    Biochemical and Biophysical Research Communications   258 ( 1 )   109 - 112   1999年4月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Academic Press Inc.  

    DOI： 10.1006/bbrc.1999.0594

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  • Role of Pax-5 in the regulation of a mid-hindbrain organizer's activity

    Jun ichi Funahashi, Jun ichi Funahashi, Tatsuya Okafuji, Hideyo Ohuchi, Sumihare Noji, Hideaki Tanaka, Harukazu Nakamura, Harukazu Nakamura

    Development Growth and Differentiation   41   59 - 72   1999年2月

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  • Structure and expression of a novel human FGF, FGF-19, expressed in the fetal brain 査読

    Tetsuya Nishimura, Yukari Utsunomiya, Masamitsu Hoshikawa, Hideyo Ohuchi, Nobuyuki Itoh

    Biochimica et Biophysica Acta - Gene Structure and Expression   1444 ( 1 )   148 - 151   1999年1月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/S0167-4781(98)00255-3

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  • FGFR2 signaling in normal and limbless chick limb buds 査読

    Gail Lizarraga, Deborah Ferrari, Michael Kalinowski, Hideyo Ohuchi, Sumihare Noji, Robert A. Kosher, Caroline N. Dealy

    Developmental Genetics   25 ( 4 )   331 - 338   1999年

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    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley-Liss Inc.  

    DOI： 10.1002/(SICI)1520-6408(1999)25:4<331::AID-DVG7>3.0.CO;2-U

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  • Pitx2, a bicoid-type homeobox gene, is involved in a lefty-signaling pathway in determination of left-right asymmetry

    Hidefumi Yoshioka, Chikara Meno, Kazuko Koshiba, Minoru Sugihara, Hiroyuki Itoh, Yoshiyasu Ishimaru, Takashi Inoue, Hideyo Ohuchi, Elena V. Semina, Jeffrey C. Murray, Hiroshi Hamada, Sumihare Noji

    Cell   94   299 - 305   1998年8月

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  • Correlation of wing-leg identity in ectopic FGF-induced chimeric limbs with the differential expression of chick Tbx5 and Tbx4. 査読

    Ohuchi H, Takeuchi J, Yoshioka H, Ishimaru Y, Ogura K, Takahashi N, Ogura T, Noji S

    Development   125 ( 1 )   51 - 60   1998年1月

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    担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • Essential roles of the winged helix transcription factor MFH-1 in aortic arch patterning and skeletogenesis 査読

    K Iida, H Koseki, H Kakinuma, N Kato, Y Mizutani-Koseki, H Ohuchi, H Yoshioka, S Noji, K Kawamura, Y Kataoka, F Ueno, M Taniguchi, N Yoshida, T Sugiyama, N Miura

    DEVELOPMENT   124 ( 22 )   4627 - 4638   1997年11月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • Pax-6 is involved in the specification of hindbrain motor neuron subtype

    Noriko Osumi, Noriko Osumi, Arisa Hirota, Hideyo Ohuchi, Masato Nakafuku, Tadahiro Iimura, Shigeru Kuratani, Michio Fujiwara, Sumihare Noji, Kazuhiro Eto

    Development   124   2961 - 2972   1997年9月

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  • Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation

    Naoki Kinto, Naoki Kinto, Masahiro Iwamoto, Motomi Enomoto-Iwamoto, Sumihare Noji, Hideyo Ohuchi, Hidefumi Yoshioka, Hiroko Kataoka, Yasuhiro Wada, Yasuhiro Wada, Gao Yuhao, Hideaki E. Takahashi, Shusaku Yoshiki, Akira Yamaguchi

    FEBS Letters   404   319 - 323   1997年3月

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  • Corelation between Distal-less expression patterns and structures of appendages in development of the two-soptted cricket, Gryllus bimaculatus

    Nao Niwa, Mariko Saitoh, Hideyo Ohuchi, Hidefumi Yoshioka, Sumihare Noji

    Zool. Sci.   14 ( 1 )   115 - 125   1997年

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    記述言語：英語   出版者・発行元：社団法人 日本動物学会  

    A million of insect species have been identified so far, displaying a staggering variety of adult morphologies. To elucidate mechanism how such insect morphologies are developed at a molecular level, we investigated developmental process of the two-spotted cricket, Gryllus bimaculatus, as a typical hemimetabola, and compared with that of Drosophila as an extensively studied typical holometabola. We analyzed expression patterns of homeobox genes of engrailed (en) and Distal-less (Dll) during development. In early embryos, en is expressed in the posterior compartments of body segments and developing appendages, while Dll is expressed in the distal region corresponding to the telopodite of developing appendages. Interestingly, these expression patterns are very similar to those observed in Drosophila imaginal discs. In the case of Dll, we found that its expression pattern, which is similar to each other in various appendages at early stages, changes in progress with elongation and segmentation, depending on the type of appendages. Late expression patterns of DII are classified into three types : Dll expression in the entire region of the antenna, in a distal region of the cercus, and in distal and middle regions of the leg, maxillary and labial palpus, indicating that Dll expression patterns are closely related to segmentation patterns of the appendages. Furthermore, since Dll is intensely expressed in both sides of the femur-tibia articulation of the leg, we considered that Dll is involved in positioning of articulation during the late appendage development. Hence, our results indicated that although common molecules are involved in development of insect appendages, the variety of the morphologies depends on pattern and timing of their expressions.

    DOI： 10.2108/zsj.14.115

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    その他リンク： http://dl.ndl.go.jp/info:ndljp/pid/10861262

  • INDUCTION OF AN ADDITIONAL LIMB BY FGF AND DUPLICATION OF DIGITS BY SONIC HEDGEHOG 査読

    H OHUCHI, H YOSHIOKA, S NOJI

    SEIKAGAKU   67 ( 10 )   1232 - 1236   1995年10月

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    担当区分：筆頭著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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  • NEW GENE, NEL, ENCODING A M(R) 93-K PROTEIN WITH EGF-LIKE REPEATS IS STRONGLY EXPRESSED IN NEURAL TISSUES OF EARLY-STAGE CHICK-EMBRYOS

    S MATSUHASHI, S NOJI, E KOYAMA, F MYOKAI, H OHUCHI, S TANIGUCHI, K HORI

    DEVELOPMENTAL DYNAMICS   203 ( 2 )   212 - 222   1995年6月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

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  • FGF Can Induce Outgrowth of Somatic Mesoderm both Inside and Outside of Limb-Forming Regions

    Tatsuo Mima, Hideyo Ohuchi, Sumihare Noji, Takashi Mikawa

    Developmental Biology   167   617 - 620   1995年2月

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  • Involvement of growth factors and their receptors in morphogenesis of the chick limb

    T. Nohno, H. Ohuchi, H. Yoshioka, S. Noji

    Gann Monographs on Cancer Research   42   79 - 90   1994年1月

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  • Differential expression of three chick FGF receptor genes, FGFR1, FGFR2 and FGFR3, in limb and feather development.

    S. Noji, E. Koyama, F. Myokai, T. Nohno, H. Ohuchi, K. Nishikawa, S. Taniguchi

    Progress in clinical and biological research   383 B   645 - 654   1993年12月

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  • A MUTATION IN THE PAX-6 GENE IN RAT SMALL-EYE IS ASSOCIATED WITH IMPAIRED MIGRATION OF MIDBRAIN CREST CELLS 査読

    T MATSUO, N OSUMIYAMASHITA, S NOJI, H OHUCHI, E KOYAMA, F MYOKAI, N MATSUO, S TANIGUCHI, H DOI, S ISEKI, Y NINOMIYA, M FUJIWARA, T WATANABE, K ETO

    NATURE GENETICS   3 ( 4 )   299 - 304   1993年4月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/ng0493-299

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    その他リンク： http://orcid.org/0000-0003-0548-6452

  • Expression pattern of fibroblast growth factor receptor 1 gene during chickeye development 査読

    H. Ohuchi

    Journal of Japanese Ophthalmological Society   97 ( 3 )   304 - 309   1993年

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    記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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  • Expression pattern of fibroblast growth factor receptor 2 gene during chick eye development 査読

    H. Ohuchi

    Journal of Japanese Ophthalmological Society   97 ( 5 )   563 - 568   1993年

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    記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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  • EXPRESSION PATTERNS OF THE ACTIVIN RECEPTOR IIA AND IIB GENES DURING CHICK LIMB DEVELOPMENT

    T NOHNO, S NOJI, E KOYAMA, F MYOKAI, H OHUCHI, K NISHIKAWA, S SUMITOMO, S TANIGUCHI, T SAITO

    LIMB DEVELOPMENT AND REGENERATION, PT B   383   705 - 714   1993年

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    記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）  

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▼全件表示

書籍等出版物

  • The Cricket as a Model Organism

    Hideyo Ohuchi, Tetsuya Bando, Taro Mito, Sumihare Noji（ 担当： 分担執筆 ,  範囲: Chapter 4: Eye Development and Photoreception of a Hemimetabolous Insect, Gryllus bimaculatus）

    Springer Japan KK  2017年 

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  • 光と生命の事典（編集：真嶋哲朗、飯野盛利、七田芳則、藤堂剛）

    大内淑代（ 担当： 分担執筆 ,  範囲: 脳内光受容体）

    朝倉書店  2016年2月  （ ISBN:9784254171617 ）

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    総ページ数：422頁   担当ページ：180頁-181頁   記述言語：日本語 著書種別：事典・辞書

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  • 発生生物学 生物はどのように形づくられるか

    Lewis Wolpert 著, 大内淑代, 野地澄晴（ 担当： 共訳）

    丸善出版  2013年7月  （ ISBN:9784621086896 ）

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  • マウス表現型解析 失敗しないステップ・バイ・ステップアプローチ Mouse Phenotypes, A Handbook of Mutation Analysis, by V. E. Papaioannou & R. R. Behringer, CSHL Press

    大内淑代, 井関祥子（ 担当： 共訳）

    メデイカルサイエンスインターナショナル  2006年 

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MISC

  • Congenital eye anomalies: More mosaic than thought? 査読 国際誌

    Ohuchi H, Sato K, Habuta M, Fujita H, Bando T

    Congenital anomalies   59 ( 3 )   56 - 73   2019年5月

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    担当区分：筆頭著者,　責任著者   記述言語：英語  

    DOI： 10.1111/cga.12304

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  • Fibroblast-growth-factor-induced additional limbs in the study of initiation of limb formation, limb identity, myogenesis, and innervation 招待 査読

    Hideyo Ohuchi, Sumihare Noji

    Cell and Tissue Research   296 ( 1 )   45 - 56   1999年

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    担当区分：筆頭著者   記述言語：英語  

    DOI： 10.1007/s004410051265

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  • An additional limb can be induced from the flank of the chick embryo by FGF4 査読

    Hideyo Ohuchi, Takashi Nakagawa, Masaki Yamauchi, Takeshi Ohata, Hidefumi Yoshioka, Takashi Kuwana, Tatsuo Mima, Takashi Mikawa, Tsutumu Nohno, Sumihare Noji

    Biochemical and Biophysical Research Communications   209 ( 3 )   809 - 816   1995年4月

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    担当区分：筆頭著者   記述言語：英語  

    DOI： 10.1006/bbrc.1995.1572

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  • Involvement of androgen-induced growth factor (FGF-8) gene in mouse embryogenesis and morphogenesis 査読

    Hideyo Ohuchi, Hidefumi Yoshioka, Akira Tanaka, Yasuhiko Kawakami, Tsutomu Nohno, Sumihare Noji

    Biochemical and Biophysical Research Communications   204 ( 2 )   882 - 888   1994年10月

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    担当区分：筆頭著者   記述言語：英語  

    DOI： 10.1006/bbrc.1994.2542

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  • Expression patterns of two fibroblast growth factor receptor genes during early chick eye development 査読

    Ohuchi Hideyo, Koyama Eiki, Myokai Fumio, Nohno Tsutomu, Shiraga Fumio, Matsuo Toshihiko, Matsuo Nobuhiko, Taniguchi Shigehiko, Noji Sumihare

    Exp Eye Res   58 ( 6 )   649 - 658   1994年6月

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    担当区分：筆頭著者  

    DOI： 10.1006/exer.1994.1062

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  • EXPRESSION PATTERN OF THE ACTIVIN RECEPTOR TYPE-IIA GENE DURING DIFFERENTIATION OF CHICK NEURAL TISSUES, MUSCLE AND SKIN 査読 国際誌

    H OHUCHI, S NOJI, E KOYAMA, F MYOKAI, K NISHIKAWA, T NOHNO, K TASHIRO, K SHIOKAWA, N MATSUO, S TANIGUCHI

    FEBS LETTERS   303 ( 2-3 )   185 - 189   1992年6月

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    担当区分：筆頭著者   記述言語：英語  

    DOI： 10.1016/0014-5793(92)80515-I

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  • コオロギ再生においてDual oxidaseが産生する活性酸素は細胞増殖と血球遊走を制御する

    板東哲哉, 奥村美紗, 大内淑代

    第47回日本分子生物学会年会抄録集   2024年11月

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  • DKK3/REIC様分子の単球系白血病細胞株THP-1の樹状細胞分化への影響

    藤田洋史, 佐藤恵太, 木下理恵, 阪口政清, 大内淑代

    第47回日本分子生物学会年会抄録集   2024年11月

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  • Fgf10遺伝子片アレル欠損マウスのハーダ腺の解析

    池田志織, 佐藤恵太, 藤田洋史, 皆木瞳, 宮石智, 濃野勉, 大内淑代

    日本解剖学会第78回中国・四国支部学術集会抄録集   2024年10月

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  • Fgf10ヘテロ接合性機能喪失マウスのハーダー腺表現型解析

    池田志織, 佐藤恵太, 藤田洋史, 皆木瞳, 大内淑代

    第12回Tokyo Vertebrate Morphology Meeting (TVMM)抄録集   2024年9月

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  • The Medaka Mutant Deficient in Eyes Shut Homolog Exhibits Opsin Transport Defects and Enhanced Autophagy in Retinal Photoreceptors

    Keita Sato, Yang Liu, Takahiro Yamashita, ○Hideyo Ohuchi

    The 21th Congress of the International Federation of Associations of Anatomists (IFAA 2024), Abstract Book   2024年9月

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  • Fgf10ヘテロ接合性変異マウスのハーダー腺の表現型解析

    池田志織, 佐藤恵太, 藤田洋史, 皆木瞳, 宮石智, 濃野勉, 大内淑代

    第57回日本発生生物学会大会抄録集   2024年6月

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  • Fgf10欠損マウスの眼球形状の解析

    池田志織, 佐藤恵太, 大内淑代

    日本先天異常学会学術集会プログラム・抄録集   64th   2024年

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  • 「視覚器の発生」

    大内淑代

    日本先天異常学会学術集会プログラム・抄録集   64th   2024年

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  • マウスハーダー腺形成機構の解明:Fgf10ヘテロ機能喪失マウスの解析

    大内淑代, 佐藤恵太, 池田志織, 皆木瞳

    日本解剖学会総会・全国学術集会抄録集(CD-ROM)   129th   2024年

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  • SABER-FISH法による光受容タンパク質オプシン発現細胞の分子組織化学的解析

    佐藤恵太, 大内淑代

    日本解剖学会総会・全国学術集会抄録集(CD-ROM)   129th   2024年

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  • カリウムチャネル遺伝子KCNJ13ノックアウト網膜色素上皮では酸化ストレスにより細胞死を生じる

    大内淑代, 神崎勇希, 神崎勇希, 藤田洋史, 佐藤恵太, 細川海音, 松前洋, 森實祐基, 森實祐基

    日本先天異常学会学術集会プログラム・抄録集   63rd   2023年7月

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    記述言語：日本語  

    J-GLOBAL

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  • Clec4a2/Clec4dダブルノックアウトは破骨細胞の分化を促進する

    藤田洋史, 高橋憲司, 田井雄馬, 上田悠都, 大内淑代

    日本生化学会大会(Web)   96th   2023年

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  • 公共scRNA-seqデータの再解析に基づくメダカ下垂体の組織化学的解析

    佐藤恵太, 大内淑代

    日本下垂体研究会学術集会プログラム・講演要旨集   37th   2023年

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  • 口唇腺のSpatial解析によるシェーグレン症候群の予後予測因子解明

    皆木瞳, 高畠清文, 伊原木聰一郎, 飯田征二, 長塚仁, 阪井丘芳, 大内淑代

    日本口腔科学会学術集会プログラム・抄録集   77th   2023年

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  • 網膜色素変性症原因遺伝子eyes shut homologノックアウトメダカ網膜に見られるオートファジーの活性化

    佐藤恵太, YANG Liu, 山下高廣, 大内淑代

    日本解剖学会総会・全国学術集会抄録集(CD-ROM)   128th   2023年

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  • CRISPR-Cas9を用いたC-type lectin receptor多重変異マウスの作製の試み-破骨細胞分化における分子機能解明を目指して-

    藤田洋史, 田井雄馬, 上田悠都, 大内淑代

    日本解剖学会総会・全国学術集会抄録集(CD-ROM)   128th   2023年

    　詳細を見る

  • SABER-FISH法を用いたメダカ下垂体における遺伝子発現の解析

    佐藤恵太, 大内淑代

    日本下垂体研究会学術集会プログラム・講演要旨集   36th   2022年

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  • ヒト脳組織におけるオプシン5局在同定の試み

    大内淑代, 佐藤恵太, 宮石智, 七田芳則, 山下高廣, TUOMINEN Hannu, SAARELA Seppo, TIMONEN Markku

    日本解剖学会総会・全国学術集会抄録集(CD-ROM)   127th   2022年

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  • 高感度蛍光in situハイブリダイゼーション法による鶏眼球壁におけるOpn5m mRNA検出の試み

    佐藤恵太, 大内淑代

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集   63rd (CD-ROM)   2022年

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  • Toll受容体と活性酸素による昆虫の器官再生の制御

    板東哲哉, 奥村美紗, 大内淑代

    日本解剖学会総会・全国学術集会抄録集(CD-ROM)   127th   2022年

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  • 涙器の細胞組織学と組織再生治療

    大内淑代

    日本眼感染症学会・日本眼炎症学会・日本コンタクトレンズ学会総会・日本涙道・涙液学会プログラム・講演抄録集   58th-55th-64th-10th   2022年

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  • Dkk3(REIC)ノックアウトマウス副腎の形態学的解析

    土生田宗憲, 藤田洋史, 佐藤恵太, 板東哲哉, 公文裕巳, 大内淑代

    日本解剖学会総会・全国学術集会抄録集(CD-ROM)   127th   2022年

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  • 「光受容体」Opn5が示す多様な分子機能

    佐藤 恵太, 山下 高廣, 大内 淑代, 七田 芳則

    生物物理   59 ( 3 )   132 - 136   2019年

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    記述言語：日本語   出版者・発行元：一般社団法人 日本生物物理学会  

    <p>Opsins are animal photoreceptor proteins that use retinal as their chromophore (ligand) and are classified into several groups based on sequence similarity. Opn5 forms one phylogenetically separate opsin group and includes three vertebrate-specific subgroups, Opn5m, Opn5L1 and Opn5L2. In this article, we introduce highly diversified functionalities of vertebrate Opn5. Three Opn5 subgroups show characteristic spectral sensitivity, preference for retinal isomers and photoreaction process, and work as bistable photoreceptor, retinal chemoreceptor or unidirectional photoreceptor. Such broad spectrum of functionalities may be attributed to optimization for respective physiological functions.</p>

    DOI： 10.2142/biophys.59.132

    CiNii Article

    CiNii Books

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    その他リンク： http://search.jamas.or.jp/link/ui/2019266724

  • グルタチオンは破骨細胞形成と炎症性骨破壊を促進する

    藤田洋史, 越智正彦, 大野充昭, 青山絵理子, 荻野哲也, 大内淑代

    日本酸化ストレス学会学術集会プログラム・抄録集   72nd   2019年

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  • The Medaka Mutant Deficient in Eyes Shut Homolog Exhibits Opsin Transport Defects and Enhanced Autophagy in Retinal Photoreceptors

    Keita Sato, Yang Liu, Takahiro Yamashita, ○Hideyo Ohuchi

    The 21th Congress of the International Federation of Associations of Anatomists (IFAA 2024), Abstract Book   2016年9月

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  • 組織形成因子 Fgf10 招待

    大内淑代

    岡山医学会雑誌   125 ( 3 )   229 - 234   2013年12月

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    記述言語：日本語   掲載種別：記事・総説・解説・論説等（大学・研究所紀要）   出版者・発行元：岡山医学会  

    DOI： 10.4044/joma.125.229

    CiNii Article

    CiNii Books

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    その他リンク： http://ousar.lib.okayama-u.ac.jp/51892

  • Molecular Aspects of Eye Evolution and Development: From the Origin of Retinal Cells to the Future of Regenerative Medicine 査読

    Hideyo Ohuchi

    ACTA MEDICA OKAYAMA   67 ( 4 )   203 - 212   2013年8月

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    記述言語：英語   掲載種別：書評論文，書評，文献紹介等  

    DOI： 10.18926/AMO/51064

    Web of Science

    PubMed

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  • Wakayama Symposium: Epithelial-Mesenchymal Interactions in Eyelid Development 査読

    Hideyo Ohuchi

    OCULAR SURFACE   10 ( 4 )   212 - 216   2012年10月

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  • The advent of RNA interference in Entomology

    Taro Mito, Taro Nakamura, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji

    ENTOMOLOGICAL SCIENCE   14 ( 1 )   1 - 8   2011年1月

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    記述言語：英語   掲載種別：書評論文，書評，文献紹介等  

    DOI： 10.1111/j.1479-8298.2010.00408.x

    Web of Science

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  • 眼の発生における線維芽細胞増殖因子遺伝子(Fgf4,Fgf8)の発現部位と機能

    大内 淑代

    日本眼科紀要 = Folia ophthalmologica Japonica   48 ( 9 )   1072 - 1076   1997年9月

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講演・口頭発表等

  • 生殖発生発達教育セミナー「視覚器の発生」 招待

    大内淑代

    第64回日本先天異常学会学術集会  2024年7月26日 

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    開催年月日： 2024年7月26日 - 2024年7月27日

    記述言語：日本語   会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

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  • 涙器の細胞組織学と組織再生治療 招待

    大内淑代

    第10回日本涙道・涙液学会総会  2022年7月9日 

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    開催年月日： 2022年7月9日 - 2022年7月10日

    記述言語：日本語   会議種別：口頭発表（招待・特別）  

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  • 神経、内分泌系に発現する光受容タンパク質の分子組織学的解析 招待

    大内淑代, 佐藤恵太

    異分野融合による次世代光生物学研究会  2019年11月7日 

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    記述言語：日本語   会議種別：口頭発表（招待・特別）  

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  • 非視覚性光受容タンパク質の機能解明と モデル動物を用いた眼疾患研究 招待

    大内淑代

    第22回眼科分子生物学研究会  2018年3月11日 

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    記述言語：日本語   会議種別：口頭発表（招待・特別）  

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  • REIC/DKK3 Expression in Mouse Normal Tissues 招待

    大内淑代

    第４回東アジア遺伝子治療推進懇話会  2015年6月21日 

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    記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

    開催地：Okayama, Japan  

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共同研究・競争的資金等の研究

  • Fgf10ハプロ不全の表現型バリエーションを生む転写ノイズ可視化の試み

    研究課題/領域番号：24K09998  2024年04月 - 2027年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    大内 淑代, 佐藤 恵太

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    配分額：4680000円 （ 直接経費：3600000円 、 間接経費：1080000円 ）

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  • 網膜硝子体界面疾患における膜形成の病態の多面的解析

    研究課題/領域番号：24K12806  2024年04月 - 2027年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    森實 祐基, 大内 淑代, 谷岡 真樹

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    配分額：4680000円 （ 直接経費：3600000円 、 間接経費：1080000円 ）

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  • 網膜色素上皮細胞におけるカリウムチャネルKir7.1の機能解明

    研究課題/領域番号：21K09700  2021年04月 - 2024年03月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    森實 祐基, 大内 淑代

    　 詳細を見る

    配分額：4030000円 （ 直接経費：3100000円 、 間接経費：930000円 ）

    網膜色素上皮細胞(以下RPE)は網膜の恒常性維持を司る細胞であり、RPEの障害によって視覚障害の主原因である網膜色素変性や加齢黄斑変性等の網膜変性疾患を発症する。しかし網膜変性疾患の病態は未解明で根本的な治療法は存在しない。そのため、RPE障害の原因を解明することは喫緊の課題である。近年、網膜変性疾患の一つであるレーバー先天盲の病態に膜電位の維持に重要なカリウムチャネルの一つ(Kir7.1)が関与することが明らかにされた。しかし、RPEに多種存在するカリウムチャネルの中でKir7.1の障害がRPE障害を引き起こす機構については不明である。そこで申請者は、RPEにおけるKir.7.1には、膜電位の維持以外の未知の機能があるのではないかとの仮説を立て、独自に作成したKir7.1欠損iPS-RPEを用いてKir.7.1の機能解明に取り組む。
    Kir7.1欠損iPS-RPEは野生型iPS-RPEと比較して細胞死を生じやすい。我々はKir7.1欠損iPS-RPEの細胞死のメカニズムの一つにRPEの酸化ストレスに対する脆弱性が関与している可能性があると考えた。トランスウェルに4週間播種した野生型およびKir7.1欠損iPS-RPEに対して酸化刺激として種々の濃度(1.5mM, 5mM, 15mM)のt-BHP(tert-ブチルヒドロペルオキシド)を培地中に添加し、MTSアッセイを行い、細胞死の定量解析を行った。t-BHPを添加したKir7.1欠損iPS-RPEは同濃度のt-BHPで刺激した野生型iPS-RPEと比較して有意に細胞死が多かった。Kir7.1欠損iPS-RPEは酸化ストレスに対する脆弱性が原因となって、細胞死を生じている可能性が示唆された。

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  • 眼球伸長におけるFGF10と紫光Opsin5システムの機能解明

    研究課題/領域番号：20K21655  2020年07月 - 2024年03月

    日本学術振興会  科学研究費助成事業 挑戦的研究(萌芽)  挑戦的研究(萌芽)

    大内 淑代, 藤田 洋史, 佐藤 恵太

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    配分額：6370000円 （ 直接経費：4900000円 、 間接経費：1470000円 ）

    本研究では、眼球が前後軸に伸長することが原因となる軸性近視の病態を解明するために、環境因子として紫光とそれを受容するオプシン５光受容体(Opn5)、遺伝因子としてFgf10に着目している。強膜や角膜が直接、紫光を受容しOpn5 GPCRシグナル系を介して、眼球壁のFgf10-MAP kinaseシグナル系を動かす、細胞外基質等の遺伝子発現が変わり、眼軸伸長が抑制されるという仕組みを検証する。これまで眼球壁におけるOpn5 mRNAの局在は、PCR法による検出と免疫組織化学的同定に依っている。本年度は、免疫組織化学の結果を証明するために、最近開発された高感度mRNA in situハイブリダイゼーション法を試みた。SABER-FISH法を用いて、ニワトリ17日胚網膜におけるOpn5、メダカ網膜におけるGad1b, メダカ下垂体におけるPomcの各mRNAの蛍光シグナル検出に成功した。よって今後、核酸オリゴマー側鎖の増幅条件を検討することで、ヒト眼組織を用いたFgf10および Opn5の局在解析を行う。本研究では、ヒトiPS細胞を用いてFgf10の眼球伸長における機能を解明する。関連研究としてヒトiPS細胞にゲノム編集を施して作製した先天性網膜変性疾患のモデルRPE細胞の病態解析を継続している（研究成果の一部は、doi.org/10.1167/iovs.61.5.38にて発表した）。また、Fgf10発現低下マウス作製を行なってFgf10シグナルの眼球伸長における役割を明らかにする。その基盤技術となるFgf10ゲノム編集初代マウスの遺伝子―表現型相関について成果発表した（Habuta M et al., 2020）。

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  • ゲノム編集技術を用いた網膜における長寿遺伝子の機能解明：加齢黄斑変性の予防戦略

    研究課題/領域番号：18K09410  2018年04月 - 2021年03月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    森實 祐基, 大内 淑代, 米澤 朋子

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    配分額：4420000円 （ 直接経費：3400000円 、 間接経費：1020000円 ）

    加齢黄斑変性(Age-related macular degeneration,以下AMD)は失明に至る難治性の眼疾患であり,加齢を背景とした網膜色素上皮細胞(以下RPE)の機能低下(老化)が原因で発症する。申請者はこれまでに,長寿遺伝子の一つであるAMP活性化プロテインキナーゼ(以下AMPK)に着目し,その活性を薬剤によって変化させ、AMPKの活性化が様々な眼疾患の病態を改善することを明らかにしてきた。しかし,AMPKやその他の長寿遺伝子が網膜やRPEの老化に及ぼす影響は不明であり,また、薬剤の非特異的作用のために,長寿遺伝子の真の機能を検討することが困難であるという問題があった。そこで本研究では,主要な長寿遺伝子であるサーチュイン(以下SIRT)とAMPKに着目し,これらの遺伝子が網膜やRPEの老化に及ぼす影響を,ゲノム編集技術を用いて作成した遺伝子改変RPEおよびマウスを用いて明らかにする。そして,十分な治療法が存在しない加齢黄斑変性の新規予防法を開発するための基盤となる成果を得る。
    上記の目的を達成するために当該年度は、SIRT遺伝子に着目し、CRISPR/Cas9等のゲノム編集技術を用いてiPS細胞のSIRT遺伝子のゲノム編集に着手した。iPS細胞のゲノム編集の前段階として、ゲノム編集の効果を確認するために、培養が簡便でゲノム編集を高い確率で行うことができる細胞を用いてゲノム編集を行った。

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  • 外的ストレスによるiPS由来網膜色素上皮細胞障害の機構解明

    研究課題/領域番号：16K11287  2016年04月 - 2019年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    木村 修平, 大内 淑代, 白神 史雄, 森實 祐基, 戸島 慎二, 荒木 亮一

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    配分額：4810000円 （ 直接経費：3700000円 、 間接経費：1110000円 ）

    網膜疾患が網膜色素上皮細胞 (RPE) の機能に及ぼす影響を検討するため、種々のストレスを負荷し、RPEの障害に関与する因子を検討し、網膜疾患に対する新たな治療法開発の基盤となる研究を行った。
    RPEに対する 組織プラスミノーゲン活性化因子 (tPA) の毒性によるストレス負荷の検討では、tPAは低濃度短時間(83μg/ml, 6時間)ではRPEに対して毒性を示さなかったが、濃度および時間依存性に毒性が増加した。tPAによる細胞毒性の原因として添加剤であるL-arginineが考えられた。

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  • 胚盤胞補完法を用いたin vivo肺再生

    研究課題/領域番号：15K15320  2015年04月 - 2017年03月

    日本学術振興会  科学研究費助成事業  挑戦的萌芽研究

    西條 康夫, 﨑村 建司, 大内 淑代, 笹岡 俊邦, 小田 佳奈子

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    配分額：3510000円 （ 直接経費：2700000円 、 間接経費：810000円 ）

    CRISPR/Cas法を用いてFgf10exon1欠損マウスを作製し、ホモ欠損マウスで肺欠損を確認した。肺欠損マウス胚にES細胞を移入して、ES細胞由来の肺の作製を試みた。まず、ヘテロマウス同士を交配し、ホモ欠損マウス胚を含む胚にES細胞およびiPS細胞を胚盤胞補完法を用いて移入した。その結果、キメラマウスの全てに肺の発生が確認された。肺はGFP陽性で、胚盤胞補完法でES細胞由来の肺が作出可能であることが強く示唆された。また、FGF10欠損マウスがホモ欠損であることを証明するため、Fgf10exon3欠損マウスを作出し、複合ヘテロFgf10exon1/exon3マウスの作出を現在行っている。

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  • 脳の"眼"を構成するオプシン5視細胞の機能解明とその発生分化メカニズム

    研究課題/領域番号：24500382  2012年04月 - 2015年03月

    文部科学省  科学研究費補助金(基盤研究(C))  基盤研究(C)

    大内淑代

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    担当区分：研究代表者  資金種別：競争的資金

    配分額：5200000円 （ 直接経費：4000000円 、 間接経費：1200000円 ）

    紫外光感受性視物質であるオプシン５(Opn5)発現細胞の生体内での機能と発生分化について、鳥類と哺乳類を用いて研究を行っている。本研究では、人工核酸切断酵素によるゲノム編集法を用いて、動物個体においてOpn5遺伝子を改変して、脳と眼におけるOpn5の機能と発生分化のしくみを明らかにすることを目的とした。人工核酸切断酵素として、Transcription Activator-Like Effector Nuclease (TALEN)を用いて、マウスOpn5変異マウスの作出を行った。その結果、TALEN法により遺伝子変異が効率よくおこること、Opn5変異マウスは正常に産出されることがわかった。

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担当授業科目

  • 人体の構造：入門 （2024年度） 特別  - その他

  • 人体構造学 （2024年度） 集中  - その他

  • 医学セミナー(テュートリアル) （2024年度） 第１学期  - 火2～3

  • 医学入門(医学といのち) （2024年度） 第３学期  - 木5～6

  • 発生学 （2024年度） 特別  - その他

  • 細胞組織学 （2024年度） 特別  - その他

  • 細胞組織学実習 （2024年度） 特別  - その他

  • 細胞組織学実習 （2024年度） 特別  - その他

  • 細胞組織学演習 （2024年度） 特別  - その他

  • 細胞組織学Ｉ（演習・実習） （2024年度） 特別  - その他

  • 細胞組織学Ｉ（講義・演習） （2024年度） 特別  - その他

  • 細胞組織学ＩＩ（演習・実習） （2024年度） 特別  - その他

  • 細胞組織学ＩＩ（講義・演習） （2024年度） 特別  - その他

  • 細胞組織工学演習 （2024年度） 特別  - その他

  • 人体の構造：入門 （2023年度） 特別  - その他

  • 人体構造学 （2023年度） 集中  - その他

  • 医学セミナー(テュートリアル) （2023年度） 第１学期  - 火2～3

  • 医学セミナー（チュートリアル） （2023年度） 第１学期  - 火2～3

  • 医学入門(医学といのち) （2023年度） 第３学期  - 木5～6

  • 発生学 （2023年度） 特別  - その他

  • 細胞組織学 （2023年度） 特別  - その他

  • 細胞組織学実習 （2023年度） 特別  - その他

  • 細胞組織学実習 （2023年度） 特別  - その他

  • 細胞組織学演習 （2023年度） 特別  - その他

  • 細胞組織学Ｉ（演習・実習） （2023年度） 特別  - その他

  • 細胞組織学Ｉ（講義・演習） （2023年度） 特別  - その他

  • 細胞組織学ＩＩ（演習・実習） （2023年度） 特別  - その他

  • 細胞組織学ＩＩ（講義・演習） （2023年度） 特別  - その他

  • 細胞組織工学演習 （2023年度） 特別  - その他

  • 人体の構造：入門 （2022年度） 特別  - その他

  • 人体構造学 （2022年度） 集中  - その他

  • 医学セミナー(テュートリアル) （2022年度） 第１学期  - 火2～3

  • 医学セミナー（チュートリアル） （2022年度） 第１学期  - 火2～3

  • 発生学 （2022年度） 特別  - その他

  • 社会医歯科学 （2022年度） 集中  - その他

  • 細胞組織学 （2022年度） 特別  - その他

  • 細胞組織学実習 （2022年度） 特別  - その他

  • 細胞組織学Ｉ（演習・実習） （2022年度） 特別  - その他

  • 細胞組織学Ｉ（講義・演習） （2022年度） 特別  - その他

  • 細胞組織学ＩＩ（演習・実習） （2022年度） 特別  - その他

  • 細胞組織学ＩＩ（講義・演習） （2022年度） 特別  - その他

  • 細胞組織工学演習 （2022年度） 特別  - その他

  • 人体の構造：入門 （2021年度） 特別  - その他

  • 人体構造学 （2021年度） 集中  - その他

  • 医学セミナー(テュートリアル) （2021年度） 第１学期  - 火2～3

  • 医学セミナー（チュートリアル） （2021年度） 第１学期  - 火2～3

  • 発生学 （2021年度） 特別  - その他

  • 社会医歯科学 （2021年度） 集中  - その他

  • 細胞組織学 （2021年度） 特別  - その他

  • 細胞組織学実習 （2021年度） 特別  - その他

  • 細胞組織学Ｉ（演習・実習） （2021年度） 特別  - その他

  • 細胞組織学Ｉ（講義・演習） （2021年度） 特別  - その他

  • 細胞組織学ＩＩ（演習・実習） （2021年度） 特別  - その他

  • 細胞組織学ＩＩ（講義・演習） （2021年度） 特別  - その他

  • 細胞組織工学演習 （2021年度） 特別  - その他

  • 人体の構造：入門 （2020年度） 特別  - その他

  • 人体構造学 （2020年度） 集中  - その他

  • 医学セミナー(テュートリアル) （2020年度） 第１学期  - 火2,火3

  • 医学セミナー（チュートリアル） （2020年度） 第１学期  - 火2,火3

  • 発生学 （2020年度） 特別  - その他

  • 社会医歯科学 （2020年度） 集中  - その他

  • 細胞組織学 （2020年度） 特別  - その他

  • 細胞組織学実習 （2020年度） 特別  - その他

  • 細胞組織学Ｉ（演習・実習） （2020年度） 特別  - その他

  • 細胞組織学Ｉ（講義・演習） （2020年度） 特別  - その他

  • 細胞組織学ＩＩ（演習・実習） （2020年度） 特別  - その他

  • 細胞組織学ＩＩ（講義・演習） （2020年度） 特別  - その他

  • 細胞組織工学演習 （2020年度） 通年  - その他

  • 細胞組織工学総論 （2020年度） 通年  - その他

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