研究者詳細 - 一瀬　勇規

論文 - 一瀬　勇規

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Talaromyces wortmannii FS2 emits beta-caryophyllene, which promotes plant growth and induces resistance. 査読

Yamagiwa Y, Inagaki Y, Ichinose Y, Toyoda K, Hyakumachi M, Shiraishi T

J. Gen. Plant Pathol. 77 ( (6) ) 336 - 341 2011年

Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin 査読

Tomoyuki Konishi, Fumiko Taguchi, Masako Iwaki, Mayumi Ohnishi-Kameyama, Masanobu Yamamoto, Ikuko Maeda, Yoshihiro Nishida, Yuki Ichinose, Mitsuru Yoshida, Tadashi Ishii

CARBOHYDRATE RESEARCH 344 ( 16 ) 2250 - 2254 2009年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

The flagellin of Pseudomonas syringae pv. tabaci is a glycoprotein that contains O-linked oligosaccharides composed of rhamnosyl and 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methylglucosyl residues. These O-linked glycans are released by hydrazinolysis and then labeled at their reducing ends with 2-aminopyridine (PA). A PA-labeled trisaccharide and a PA-labeled tetrasaccharide are isolated by normal-phase high-performance liquid chromatography. These oligosaccharides are structurally characterized using mass spectrometry and NMR spectroscopy. Our data show that P. syringae pv. tabaci flagellin is glycosylated with a tetrasaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1 -> 3)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rha-(1 ->, as well a trisaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1 -> 3)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rha-(1 ->,which was identified in a previous study. (C) 2009 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.carres.2009.07.004

我が国におけるブドウ根頭がんしゅ病菌の遺伝型とその分布

川口章, 澤田宏之, 一瀬勇規

土と微生物 62 ( 2 ) 143 - 143 2008年6月

[MAMP signal transduction].

Yuki Ichinose

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 52 ( 6 Suppl ) 635 - 41 2007年5月

Post-translational modification of flagellin determines the specificity of HR induction. 査読

Fumiko Taguchi, Rena Shimizu, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Plant & cell physiology 44 ( 3 ) 342 - 9 2003年3月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OXFORD UNIV PRESS

Flagellin, a constituent of the flagellar filament, is a potent elicitor of hypersensitive cell death in plant cells. Flagellins of Pseudomonas syringae pvs. glycinea and tomato induce hypersensitive cell death in their non-host tobacco plants, whereas those of P. syringae pv. tabaci do not remarkably induce it in its host tobacco plants. However, the deduced amino acid sequences of flagellins from pvs. tabaci and glycinea are identical, indicating that post-translational modification of flagellins plays an important role in determining hypersensitive reaction (HR)-inducibility. To investigate genetically the role of modification of flagellin in HR-induction, biological and phytopathological phenotypes of a flagella-defective Delta fliC mutant and Delta fliC mutants complemented by the introduction of the flagellin gene (fliC) from different pathovars of P. syringae were investigated. The Delta fliC mutant of pv. tabaci lost flagella, motility, the ability to induce HR cell death in non-host tomato cells and virulence toward host tobacco plants, whereas all pv. tabaci complemented by the introduction of the fliC gene of pvs. tabaci, glycinea or tomato recovered all the abilities that the Delta fliC mutant had lost. These results indicate that post-translational modification of flagellins is strongly correlated with the ability to cause HR cell death.

DOI： 10.1093/pcp/pcg042

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エンドウのエリシター誘導性遺伝子発現におけるAAAGモチーフとPsDof1タンパク質の関与

關, 光, 丸谷, 瑞理, 稲垣, 善茂, 豊田, 和弘, 白石, 友紀, 一瀬, 勇規

岡山大學農學部學術報告 = Scientific report of the Faculty of Agriculture, Okayama University 92 21 - 26 2003年2月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：岡山大學農學部

エリシターを処理したエンドウ上胚軸由来のRNAから作成されたcDNAライブラリーからエリシター処理により発現が増高する遺伝子候補のcDNAとしてPsDof1が単離された。大腸菌で生産されたGST-PsDof1融合タンパク質はAAAG配列をコアとするDNAに結合することが明らかにされている。本論文では、GST-PsDof1がエリシター応答性遺伝子の一つ、PsCHS1のプロモーター上のAAAGまたはCTTT配列を有する断片に特異的に結合することを明らかにした。更にAAAG配列のエリシター応答性シスエレメントとしての機能を解析するため、AAAG配列を4回繰り返したユニットをCaMV35Sの最小プロモーターとCATレポーター遺伝子に連結したキメラ遺伝子を構築し、エンドウプロトプラストにエレクトロポレーション法により導入した。CAT活性を指標にプロモーター活性を調べたところ、AAAG配列を有するプロモーターは、エリシター処理により活性化されることが明らかとなった。これらの結果はPsDof1がエリシター応答性防御遺伝子のプロモーター上のAAAG配列に結合し、転写を活性化させる可能性を示唆している。
identifier:670005
identifier:ZZ00015342

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その他リンク： http://id.ndl.go.jp/bib/6470981

Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen 査読

Y Ishiga, A Funato, T Tachiki, K Toyoda, T Shiraishi, T Yamada, Y Ichinose

PLANT AND CELL PHYSIOLOGY 43 ( 10 ) 1210 - 1220 2002年10月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OXFORD UNIV PRESS

Suppressors produced by Mycosphaerella pinodes are glycopeptides to block pea defense responses induced by elicitors. A clone, S64, was isolated as cDNA for suppressor-inducible gene from pea epicotyls. The treatment of pea epicotyls with suppressor alone induced an increase of S64 mRNA within 1 h, and it reached a maximum level at 3 h after treatment. The induction was not affected by application of the elicitor, indicating that the suppressor has a dominant action to regulate S64 gene expression. S64 was also induced by inoculation with a virulent pathogen, M. pinodes, but not by inoculation with a non-pathogen, Ascochyta rabiei, nor by treatment with fungal elicitor. The deduced structure of S64 showed high homology to 12-oxophytodienoic acid reductase (OPR) in Arabidopsis thaliana. A recombinant protein derived from S64 had OPR activity, suggesting compatibility-specific activation of the octadecanoid pathway in plants. Treatment with jasmonic acid (JA) or methyl jasmonic acid, end products of the octadecanoid pathway, inhibited the elicitor-induced accumulation of PAL mRNA in pea. These results indicate that the suppressor-induced S64 gene expression leads to the production of JA or related compounds, which might contribute to the establishment of compatibility by inhibiting the phenylpropanoid biosynthetic pathway.

DOI： 10.1093/pcp/pcf144

cDNA cloning and characterization of tobacco ABC transporter: NtPDR1 is a novel elicitor-responsive gene. 査読国際誌

Michiko Sasabe, Kazuhiro Toyoda, Tomonori Shiraishi, Yoshishige Inagaki, Yuki Ichinose

FEBS letters 518 ( 1-3 ) 164 - 8 2002年5月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

We isolated an INF1 elicitin-inducible cDNA encoding a pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporter homolog (NtPDR1) in suspension-cultured tobacco Bright Yellow-2 (BY-2) cells by application of differential display PCR. The NtPDR1 (Nicotiana tabacum PDR protein 1) gene also encodes a 162 kDa protein that includes two putative hydrophilic domains containing the ABC signature motif and two putative hydrophobic domains. Expression of the NtPDR1 gene was rapidly and strongly activated by treatment of BY-2 cells with INF1 elicitin. Further, treatment of BY-2 cells with flagellin, a bacterial proteinaceous hypersensitive reaction elicitor, or yeast extract, a general elicitor, also induced NtPDR1 gene expression. These results indicate that NtPDR1 may be involved in the general defense response in tobacco. This is the first report that microbial elicitors induce the expression of a plant ABC transporter gene.

DOI： 10.1016/S0014-5793(02)02697-2

白石友紀, 豊田和弘, 木場章範, 一瀬勇規

化学と生物 39 ( 10 ) 686 - 692 2001年10月

Suppressors of defense - Supprescins and plant receptor molecules 査読

T Shiraishi, K Toyoda, T Yamada, Y Ichinose, A Kiba, M Sugimoto

DELIVERY AND PERCEPTION OF PATHOGEN SIGNALS IN PLANTS 112 - 121 2001年

Bacteriophage P4282, a parasite of Ralstonia solanacearum, encodes a bacteriolytic protein important for lyric infection of its host 査読

H. Ozawa, H. Tanaka, Y. Ichinose, T. Shiraishi, T. Yamada

Molecular and General Genetics 265 ( 1 ) 95 - 101 2001年

記述言語：英語掲載種別：研究論文（学術雑誌）

To enhance bacterial wilt resistance in tobacco expressing a foreign protein, we isolated the bacteriolytic gene from a bacteriophage that infects Ralstonia solanacearum. The bacteriolytic protein of phage P4282 isolated in Tochigi Prefecture was purified from a lysate of R. solanacearum M4S cells infected with the phage, and its bacteriolytic activity was assayed by following the decrease in the turbidity of suspensions of R. solanacearum M4S cells. The molecular weight of the bacteriolytic protein was approximately 71 kDa, and the sequence of the N-terminal 13 amino acids was determined. We used oligonucleotide probes based on this amino acid sequence to isolate the bacteriolytic gene from phage P4282 DNA. This gene of 2061 bp encodes a product of 687 amino acids, whose calaculated molecular weight was 70.12 kDa. The bacteriolytic gene was placed under the control of an inducible promoter, and the plasmid was transformed into Escherichia coli NM522. The soluble proteins extracted from E. coli NM522 cells harboring the plasmid with the bacteriolyric gene showed obvious bacteriolytic activities against several strains of R. solanacearum isolated in various districts in Japan. DNA fragments from five phages, isolated in Niigata, Aomori, Okinawa, Fukushima and Yamaguchi Prefectures, hybridized to the bacteriolytic gene of phage P4282. These observations indicate that the bacteriolytic protein shows nonspecific activity against R. solanacearum strains, and a sequence similar to that of the bacteriolytic gene is conserved in the DNA of other bacteriophages. These results indicate that the generation of transgenic (tobacco) plants expressing the bacteriolytic gene of phage P4282 might result in enhanced resistance to bacterial wilt in tobacco.

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Regulation of nuclear gene expression in relation to signal molecules 査読

T Yamada, Y Ichinose, T Shiraishi, K Toyoda, Y Imura, H Seki, P Sriprasertsak, A Funado

DELIVERY AND PERCEPTION OF PATHOGEN SIGNALS IN PLANTS 164 - 173 2001年

Plant cell wall with the suppressor may play a crucial role in determining specificity 査読

T Shiraishi, A Kiba, A Inata, T Sugiura, K Toyoda, Y Ichinose, T Yamada

MOLECULAR GENETICS OF HOST-SPECIFIC TOXINS IN PLANT DISEASES 13 343 - 353 1998年

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：SPRINGER

The mechanism of plant host-parasite specificity is one of the most intriguing issues of contemporary plant biology. We recently found that a fungal elicitor stimulated cell wall functions such as ATP-hydrolyzing and superoxide generating activities. Furthermore, the suppressor from Mycosphaerella pinodes inhibited these activities in vitro in a strictly species-specific manner. The cell wall-bound ATPase, which is different from that of the plasma membrane in several properties, was associated with cell wall-bound peroxidase(s). In response to the elicitor, the isolated cell walls are able to produce an infection-inhibitor that may be dependent upon the superoxide generating system. On the other baud, the suppressor inhibited such production in pea cell walls and induced it in nonhost's cell walls. Thus, the effects of both fungal signals on isolated cell walls coincide with those on plant tissues. Ln this treatise, we discuss the importance of the cell wall, which is plant-specific acid the most exterior organelle, in determining host-parasite specificity.

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Fungal signals regulate ATPase and polyphosphoinositide metabolism in pea plants 査読

T Shiraishi, T Yamada, Y Ichinose, K Toyoda, A Kiba, T Kato

MOLECULAR ASPECTS OF PATHOGENICITY AND RESISTANCE: REQUIREMENT FOR SIGNAL TRANSDUCTION 197 - 208 1996年

Regulation of genes for phenylpropanoid synthesis in pea by elicitor and suppressor 査読

T Yamada, T Shiraishi, Y Ichinose, H Kato, H Seki, Y Murakami

MOLECULAR ASPECTS OF PATHOGENICITY AND RESISTANCE: REQUIREMENT FOR SIGNAL TRANSDUCTION 151 - 162 1996年

H+-translocating Activity in Proteoliposomes Reconstituted with Pea Plasma Membrane ATPase and Its Inhibition by Fungal Suppressor from Mycosphaerella pinodes.

天野政史, 豊田和弘, 一瀬勇規, 山田哲治, 白石友紀

日本植物病理学会報 61 ( 4 ) 369 - 375 1995年

記述言語：英語出版者・発行元：The Phytopathological Society of Japan

エンドウ褐紋病菌のサプレッサーは宿主エンドウの原形質膜ATPase活性を抑制する。本報告では,可溶化・部分精製したエンドウ原形質膜ATPaseをリボソームに再構成し,ATPaseとプロトンポンプ活性に対するエリシターとサプレッサーの影響について調べた。再構成膜におけるプロトンポンプ活性はATPとMg²⁺に依存し,ATPaseの阻害剤で抑制されることからATPase依存性であることが推察できた。また,プロトンの勾配はプロトンイオノフォアやNH₄Clの添加で打ち消された。本活性に対するエリシターとサプレッサーの影響を調べたところ,エリシターは全く影響を及ぼさなかったが,サプレッサーは濃度依存的に活性を抑制した。さらに,ホスファチジルイノシトール2リン酸は本活性を上昇させたが,その作用点はサプレッサーの作用点とは異なることが示唆された。これらの結果から,エンドウ褐紋病菌はサプレッサーを分泌し,エンドウ細胞におけるホメオスタシスを攪乱することにより抵抗反応を抑制し,感染に成功するものと考察した。

DOI： 10.3186/jjphytopath.61.369

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Effect of methyl jasmonate on harpin-induced hypersensitive cell heath, generation of hydrogen peroxide and expression of PAL mRNA in tobacco suspension cultured BY-2 cells 査読

S Andi, F Taguchi, K Toyoda, T Shiraishi, Y Ichinose

PLANT AND CELL PHYSIOLOGY 42 ( 4 ) 446 - 449 1992年4月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OXFORD UNIV PRESS

Methyl jasmonate inhibited the harpin-induced defense responses such as cell death, H2O2 generation and gene expression encoding phenylalanine ammonia-lyase in tobacco suspension cultured BY-2 cells, These results suggest that MeJA may act as an endogenous suppressor for plant defense response including hypersensitive reaction.

DOI： 10.1093/pcp/pce056

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