Faculty Profiles - FUNAHASHI Hiroaki

Papers - FUNAHASHI Hiroaki

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γ-glutamyl transpeptidase of spermatozoa may decrease oocyte glutathione content at fertilization in pigs Reviewed

Hiroaki Funahashi, Zoltan Machaty, Randall S. Prather, Billy N. Day

Molecular Reproduction and Development 45 ( 4 ) 485 - 490 1996.12

Microtubule Organization in Porcine Oocytes during Fertilization and Parthenogenesis1 Reviewed

Nam-Hyung Kim, Calvin Simerly, Hiroaki Funahashi, Gerald Schatten, Billy N. Day

Biology of Reproduction 54 ( 6 ) 1397 - 1404 1996.6

Presence of Organic Osmolytes in Maturation Medium Enhances Cytoplasmic Maturation of Porcine Oocytes1 Reviewed

Hiroaki Funahashi, Nam-Hyung Kim, Todd T. Stumpf, Thomas C. Cantley, Billy N. Day

Biology of Reproduction 54 ( 6 ) 1412 - 1419 1996.6

Effects of oviductal fluid on sperm penetration and cortical granule exocytosis during fertilization of pig oocytes in vitro Reviewed

N-H. Kim, H. Funahashi, L. R. Abeydeera, S. J. Moon, R. S. Prather, B. N. Day

Reproduction 107 ( 1 ) 79 - 86 1996.5

Effects of Injecting Calcium Chloride into in Vitro-Matured Porcine Oocytes1 Reviewed

Z. Macháty, H. Funahashi, M. A. Mayes, B. N. Day, R. S. Prather

Biology of Reproduction 54 ( 2 ) 316 - 322 1996.2

Microtubule and microfilament dynamics in porcine oocytes during meiotic maturation Reviewed

Nam‐Hyung Kim, Hiroaki Funahashi, Randall S. Prather, Gerald Schatten, Billy N. Day

Molecular Reproduction and Development 43 ( 2 ) 248 - 255 1996.2

Current status of in vitro production of porcine embryos Invited Reviewed

H Funahashi, BN Day

ADVANCES IN SWINE IN BIOMEDICAL RESEARCH, VOLS 1 AND 2 491 - 502 1996

Factors Affecting Development In Vitro of Bovine and Rat 1-Cell Embryos. Reviewed

Kazuchika Miyoshi, Hiroaki Funahashi, Koji Niwa

Journal of Mammalian Ova Research 13 ( 2 ) 71 - 80 1996

Low Salt Maturation Medium Enhances the Histone H1 Kinase Activity of Porcine Oocytes at the End of In Vitro Maturation. Reviewed

Hiroaki FUNAHASHI, Todd T. STUMPF, Nam-Hyung KIM, Billy N. DAY

Journal of Reproduction and Development 42 ( 2 ) 109 - 115 1996

Pronuclear formation and intracellular glutathione content ofin vitro-matured porcine oocytes followingin vitrofertilisation and/or electrical activation Reviewed

Hiroaki Funahashi, Todd T. Stumpf, Thomas C. Cantley, Nam-Hyung Kim, Billy N. Day

Zygote 3 ( 3 ) 273 - 281 1995.8

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Cambridge University Press (CUP)

Summary

Pronuclear formation and intracellular content of glutathione, containing reduced and oxidised forms, in porcine oocytes maturedin vitrowere determined following insemination and/or electrical stimulation. After insemination, sperm penetration had occurred as early as 3 h and female pronuclei had formed by 6 h with complete development by 12 h. Male pronuclear formation occurred, primarily, between 9 and 12 h after insemination. Glutathione content of the oocytes decreased following sperm penetration and remained at a depressed level until 12 h. After electrical stimulation, oocyte activation had occurred and female pronuclei had formed by 3 and 6 h, respectively. Oocyte glutathione content did not change as a result of oocyte activation. When oocytes were exposed to an electrical pulse and then spermatozoa, female pronuclear formation was observed by 3 h after stimulation/insemination. Sperm penetration was observed between 3 and 9 h. However, the incidence of male pronuclear formation observed at 12 h was extremely low, although sperm decondensation had occurred in some oocytes. Oocyte glutathione content had not decreased by 6 h following electrical activation. These results demonstrate that the changes in glutathione content in porcine oocytes following fertilisationin vitrodiffer from those due to electrical activation. Further, the decreased intracellular glutathione content in oocytes activated by sperm penetration appears to be due to the presence of a sperm factor.

DOI： 10.1017/s0967199400002677

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Pronuclear visibility, development and transgene expression in IVM/IVF-derived porcine embryos Reviewed

H.-M. Kubisch, M.A. Larson, H. Funahashi, B.N. Day, R.M. Roberts

Theriogenology 44 ( 3 ) 391 - 401 1995.8

Use of Low-Salt Culture Medium for in Vitro Maturation of Porcine Oocytes is Associated with Elevated Oocyte Glutathione Levels and Enhanced Male Pronuclear Formation after in Vitro Fertilization1 Reviewed

Hiroaki Funahashi, Thomas C. Cantley, Todd T. Stumpf, Steven L. Terlouw, Billy N. Day

Biology of Reproduction 51 ( 4 ) 633 - 639 1994.10

In Vitro Development of in Vitro-Matured Porcine Oocytes Following Chemical Activation or in Vitro Fertilization1 Reviewed

Hiroaki Funahashi, Thomas C. Cantley, Todd T. Stumpf, Steven L. Terlouw, Billy N. Day

Biology of Reproduction 50 ( 5 ) 1072 - 1077 1994.5

Different hormonal requirements of pig oocyte-cumulus complexes during maturation in vitro Reviewed

H. Funahashi, T. Cantley, B. N. Day

Reproduction 101 ( 1 ) 159 - 165 1994.5

Development of rat one-cell embryos in a chemically defined medium: effects of glucose, phosphate and osmolarity Reviewed

K. Miyoshi, H. Funahashi, K. Okuda, K. Niwa

Reproduction 100 ( 1 ) 21 - 26 1994.1

Developmental ability of porcine oocytes matured and fertilized in vitro Reviewed

H. Funahashi, T.T. Stumpf, S.L. Terlouw, T.C. Cantley, A. Rieke, B.N. Day

Theriogenology 41 ( 7 ) 1425 - 1433 1994.1

Effects of electrical stimulation before or after in vitro fertilization on sperm penetration and pronuclear formation of pig oocytes Reviewed

Hiroaki Funahashi, Todd T. Stumpf, Steve L. Terlouw, Billy N. Day

Molecular Reproduction and Development 36 ( 3 ) 361 - 367 1993.11

Effects of follicular fluid at fertilization in vitro on sperm penetration in pig oocytes Reviewed

H. Funahashi, B. N. Day

Reproduction 99 ( 1 ) 97 - 103 1993.9

Effects of the duration of exposure to hormone supplements on cytoplasmic maturation of pig oocytes in vitro Reviewed

H. Funahashi, B. N. Day

Reproduction 98 ( 1 ) 179 - 185 1993.5

Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium Reviewed

J.H. Kim, H. Funahashi, K. Niwa, K. Okuda

Theriogenology 39 ( 4 ) 875 - 886 1993.4

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