Updated on 2025/10/07

写真a

 
FUNAHASHI Hiroaki
 
Organization
Scheduled update Professor
Position
Professor
Homepage
http://www.okayama-u.ac.jp/user/agr/bunya/4/42.html
External link

Degree

  • Ms. of Agriculture ( Okayama University )

  • Ph. D. ( Okayama University )

Research Interests

  • Animal Reproductive Science

  • Animal Science

  • Gametes (Sperm and oocytes)

  • Maturation, Fertilization and early development in vitro

Research Areas

  • Life Science / Animal production science

  • Life Science / Cell biology

Education

  • Okayama University   大学院自然科学研究科   生物資源科学

    1987.4 - 1990.3

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    Country： Japan

    Notes： 博士課程

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  • Okayama University   大学院農学研究科   畜産学専攻

    1985.4 - 1987.3

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    Country： Japan

    Notes： 修士課程

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  • Okayama University   農学部   畜産学科

    1981.4 - 1985.3

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    Country： Japan

    Notes： 学士課程

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Research History

  • Okayama University   Faculty of General Education and Global Studies   Professor

    2025.4

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    Country：Japan

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  • Okayama University   Institute of Environmental, Life, Natural Science and Technology   Professor

    2023.4 - 2025.3

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    Country：Japan

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  • Okayama University, Institute of Environmental and Life Science   Professor

    2021.4 - 2023.3

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    Country：Japan

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  • Okayama University   Executive Director for Academic Affairs / Provost

    2021.4 - 2023.3

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    Country：Japan

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  • Okayama University   Vice President

    2020.4 - 2021.3

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    Country：Japan

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  • Okayama University   The Graduate School of Environmental and Life Science   Dean

    2017.4 - 2021.3

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  • Assisted Reproductive Technology Center, Okayama University   Professor

    2013.10

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    Country：Japan

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  • Graduate School of Environmental and Life Science, Okayama University   Professor

    2012.4 - 2023.3

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    Country：Japan

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  • Graduate School of Natural Science and Technology, Okayama University   Professor

    2011.1 - 2012.3

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    Country：Japan

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  • University of Murcia   Faculty of Veterinary Science   Visiting Professor

    2008.12 - 2018.12

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    Country：Spain

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  • Kurashiki Sakuyo University   Faculty of Music   Visiting Lecturer

    2000.4 - 2013.3

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    Country：Japan

    Notes：教養科目（生命の科学）担当

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  • Graduate School of Natural Science and Technology, Okayama University   Associate Professor

    2000.4 - 2010.12

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    Country：Japan

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  • Swedish University of Agricultural Sciences   Faculty of Veterinary Science   Visiting Scientist

    1999.5 - 1999.7

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    Country：Sweden

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  • National Institute for Agricultural Research (INRA)   Visiting Scientist

    1998.5 - 1998.6

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    Country：France

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  • Okayama University   Faculty of Agriculture

    1996 - 2000

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    Country：Japan

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  • Department of Animal Science, University of Missouri-Columbia   Research Assistant Professor

    1994 - 1996

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    Country：United States

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  • Department of Animal Science, University of Missouri-Columbia   Postdoctral Fellow

    1991 - 1994

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    Country：United States

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  • Central Research Institute for Feed and Livestock, National Federation of Agricultural Cooperative Association (ZEN-NOH)   Researcher

    1987 - 1991

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    Country：Japan

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Professional Memberships

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Papers

  • Resilience of porcine oocyte-cumulus complexes derived from antral follicles with various diameters to oxidative stress by BCB staining Reviewed

    Phong Ngoc Van, Takuya Wakai, Hiroaki Funahashi

    Theriogenology   246   117554 - 117554   2025.10

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.theriogenology.2025.117554

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  • Characteristics of porcine oocyte-cumulus complexes derived from various sizes of antral follicles and classified by brilliant cresyl blue staining, and developmental competence of the oocytes Reviewed

    Phong Ngoc Van, Son Quang Do, Wanniarachchige Tharindu Lakshitha Fonseka, Takuya Wakai, Hiroaki Funahashi

    Theriogenology   236   74 - 81   2025.4

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.theriogenology.2025.02.001

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  • Exogenous expression of PGC-1α during in vitro maturation impairs the developmental competence of porcine oocytes Reviewed

    Son Quang Do, Hai Thanh Nguyen, Takuya Wakai, Hiroaki Funahashi

    Theriogenology   228   30 - 36   2024.10

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.theriogenology.2024.07.021

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  • The impact of cumulus cell viability and pre-culture with the healthy cell mass on brilliant cresyl blue (BCB) staining assessment and meiotic competence of suboptimal porcine oocytes Reviewed

    Wanniarachchige Tharindu Lakshitha Fonseka, Son Quang Do, Phong Ngoc Van, Hai Thanh Nguyen, Takuya Wakai, Hiroaki Funahashi

    Theriogenology   226   158 - 166   2024.9

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.theriogenology.2024.06.012

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  • Effect of oral administration of dehydroepiandrosterone on PCOS-Like phenotype of female C57BL/6 mice Reviewed

    Ayu Mulia Sundari, Nurul Muhammad Prakoso, Astari Dwiranti, Abinawanto Abinawanto, Arief Boediono, Hiroaki Funahashi, Anom Bowolaksono

    Jurnal sain peternakan indonesia   19 ( 2 )   111 - 116   2024.7

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Badan penerbitan fakultas pertanian (BPFP) universitas bengkulu  

    We aim to evaluate the efficacy and optimal dose of orally administered DHEA in the PCOS mice model by assessing their ovarian morphology and serum FSH, LH, and testosterone levels. Female C57BL/6 mice were divided into a control group (n=5, received daily injections of 0.2 ml sesame oil) and an experimental group, which was further classified into 1) D-45 group (n=5), 2) D-60 (n=5), and 3) D-90 group (n=5) that were treated with 45, 60, and 90 mg/Kg body weight of oral DHEA. After modelling, mice in the control group had a regular estrous cycle, while mice in all experimental groups had a disturbed estrous cycle. Ovarian histology showed several growing follicles and some corpora lutea (CL) in the control, D-60, and D-90 groups. However, some large antral follicles and decreased CL were observed in the D-45 group. Serum FSH was significantly lower in the D-45 group than in the control group (3.73 ± 0.12 vs. 5.28 ± 0.31 mIU/mL, P<0.01), but D-60 and D-90 groups had a similar FSH level to the control group (P>0.05). The serum level of LH and testosterone were significantly elevated in the D-45 group than in the control group (2.52 ± 0.43 vs. 1.30 ± 0.33 mIU/mL, P<0.01 and 1.80 ± 0.32 vs. 1.24 ± 0.23 ng/mL, P<0.01, respectively). Still, the level of LH and testosterone in the D-60 and the D-90 groups was comparable to the control group (P>0.05). Our study suggests that oral administration of DHEA is efficacious in establishing PCOS-like phenotype in mice with the suggested optimal dosage of 45 mg/Kg body weight.

    DOI： 10.31186/jspi.id.19.2.111-116

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  • Hormonal and Laboratory Profile of Polycystic Ovarian Syndrome (Pcos) Women Visiting Fertility Center in Indonesia Reviewed

    Ayu Mulia Sundari, Tri Aprilliana Wulandari, Nurul Muhammad Prakoso, Astari Dwiranti, Abinawanto, Arief Boediono, Hiroaki Funahashi, Anom Bowolaksono

    Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan   18 ( 1 )   67 - 75   2024.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Fakultas Kedokteran UPN Veteran Jakarta  

    Our study aims to scrutinize the hormonal and laboratory profile of women with PCOS visiting a fertility center in Indonesia. A computerized database of 120 PCOS women and 120 controls was retrospectively reviewed and analyzed. According to analysis, the concentration of AMH and basal LH were significantly more elevated in the PCOS group than in the control group (6.77 vs. 2.05, P<0.001; and 6.8 vs. 5.3, P<0.001, respectively). The level of basal FSH was significantly lower in the PCOS group (5.83 vs. 7.86; P<0.001). After an IVF cycle, PCOS women had significantly higher retrieved oocytes and mature oocytes compared to controls (18 vs. 8, P<0.001; 12 vs. 6, P<0.001). The number of fertilizations, cleavages, and top-quality cleavages were also higher in the PCOS group than in the control group (10 vs. 4, P<0.001; 10 vs. 4, P<0.001; 4 vs. 2, P<0.001). At the blastocyst stage, patients with PCOS had a higher number of blastocysts and top-quality blastocysts than control (9 vs. 4, P<0.001); 4 vs. 1, P<0.01). However, the clinical pregnancy was significantly higher in the control group than in the PCOS group (52.6% vs. 16.7%; P = 0.045).

    DOI： 10.33533/jpm.v18i1.7546

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  • Redistribution of fragmented mitochondria ensure symmetric organelle partitioning and faithful chromosome segregation in mitotic mouse zygotes Reviewed

    Haruna Gekko, Ruri Nomura, Daiki Kuzuhara, Masato Kaneyasu, Genpei Koseki, Deepak Adhikari, Yasuyuki Mio, John Carroll, Tomohiro Kono, Hiroaki Funahashi, Takuya Wakai

    2024.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Cold Spring Harbor Laboratory  

    Abstract

    In cleavage-stage embryos, preexisting organelles partition evenly into daughter blastomeres without significant cell growth after symmetric cell division. The presence of mitochondrial DNA within mitochondria and its restricted replication during preimplantation development makes their inheritance particularly important. While chromosomes are precisely segregated by the mitotic spindle, the mechanisms controlling mitochondrial partitioning remain poorly understood. In this study, we investigate the mechanism by which Dynamin-related protein 1 (Drp1) controls the mitochondrial redistribution and partitioning during embryonic cleavage. Deletion of Drp1 in mouse zygotes causes marked mitochondrial aggregation, and the majority of embryos arrest at the 2-cell stage. Clumped mitochondria are located in the center of mitotic Drp1-depleted zygotes with less uniform distribution, thereby preventing their symmetric partitioning. Asymmetric mitochondrial inheritance is accompanied by functionally inequivalent blastomeres with biased ATP and endoplasmic reticulum Ca2+levels. We also find that marked mitochondrial centration in Drp1-depleted zygotes prevents the assembly of parental chromosomes, resulting in chromosome segregation defects and binucleation. Thus, mitochondrial fragmentation mediated by Drp1 ensure proper organelle positioning and partitioning into functional daughters during the first embryonic cleavage.

    Impact statement

    Depletion of Dynamin-related protein 1, a key regulator of mitochondrial fission, in mouse zygotes impair symmetric organelle partitioning and chromosome segregation leading to early developmental arrest.

    DOI： 10.1101/2024.06.13.598818

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  • Negative correlations of mitochondrial DNA copy number in commercial frozen bull spermatozoa with the motility parameters after thawing Reviewed International journal

    Hai Thanh Nguyen, Son Quang Do, Hiroshi Kobayashi, Takuya Wakai, Hiroaki Funahashi

    Theriogenology   210   154 - 161   2023.10

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.theriogenology.2023.07.027

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  • Photo-dependent cytosolic delivery of shRNA into a single blastomere in a mouse embryo. Reviewed International journal

    Yuka Ikawa, Takuya Wakai, Hiroaki Funahashi, Tet Htut Soe, Kazunori Watanabe, Takashi Ohtsuki

    Scientific reports   13 ( 1 )   13050 - 13050   2023.8

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    Single-cell-specific delivery of small RNAs, such as short hairpin RNA (shRNA) and small noncoding RNAs, allows us to elucidate the roles of specific upregulation of RNA expression and RNAi-mediated gene suppression in early embryo development. The photoinduced cytosolic dispersion of RNA (PCDR) method that we previously reported can introduce small RNAs into the cytosol of photoirradiated cells and enable RNA delivery into a single-cell in a spatiotemporally specific manner. However, the PCDR method has only been applied to planer cultured cells and not to embryos. This study demonstrated that the PCDR method can be utilized for photo-dependent cytosolic shRNA delivery into a single blastomere and for single blastomere-specific RNA interference in mouse embryos. Our results indicate that PCDR is a promising approach for studying the developmental process of early embryogenesis.

    DOI： 10.1038/s41598-023-40361-9

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  • Oocyte-cumulus cells crosstalk: New comparative insights Reviewed International coauthorship International journal

    Cristina A. Martinez, Dimitrios Rizos, Heriberto Rodriguez-Martinez, Hiroaki Funahashi

    Theriogenology   205   87 - 93   2023.7

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.theriogenology.2023.04.009

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  • Rapid thawing of frozen bull spermatozoa by transient exposure to 70 °C improves the viability, motility and mitochondrial health Reviewed International coauthorship International journal

    Hai Thanh Nguyen, Son Quang Do, Rukmali Athurupana, Takuya Wakai, Hiroaki Funahashi

    Animal Reproduction   20 ( 3 )   e20220127   2023

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：FapUNIFESP (SciELO)  

    DOI： 10.1590/1984-3143-ar2022-0127

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  • Anti-Müllerian hormone independently affect mtDNA copy number in human granulosa cells Reviewed International coauthorship International journal

    Anom Bowolaksono, Ayu Mulia Sundari, Muhammad Fauzi, Mila Maidarti, Budi Wiweko, Kresna Mutia, Pritta Ameilia Iffanolida, Ririn Rahmala Febri, Astari Dwiranti, Hiroaki Funahashi

    Journal of Ovarian Research   15 ( 1 )   2022.10

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    Background:

    Recently, as a delayed childbearing trend is emerging in modern women’s adulthood, diminished reproductive potential due to age-related changes is more prevalent. Reduction in the abundance of mitochondrial DNA (mtDNA) copies and circulating anti-Müllerian hormone (AMH) have been separately reported with aging, contributing to the decrease in successful reproduction. However, there are limited reports on the impact of age on mtDNA and AMH in the same individual and whether mtDNA copy numbers are influenced by age and AMH.

    Methods:

    In the present study, we utilized a real-time quantitative PCR (RT-qPCR) to quantify the mtDNA copy number of granulosa cells obtained from 43 women undergoing an in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) program.

    Results:

    According to our analysis, a significant correlation was observed between age and mtDNA copy number (r = −0.54, P < 0.001) and between age and AMH level (r = −0.48, P < 0.001) of the same individual. There was also a positive correlation between mtDNA copy number and AMH (r = 0.88, P < 0.001) with AMH level falling as mtDNA decreases. In our regression, age and AMH were shown to have low collinearity (VIF = 1.297) but only AMH was correlated with mtDNA quantity (P < 0.001).

    Conclusion:

    Our study suggests that both mtDNA and AMH abundance are influenced by age and that AMH levels independently affect mtDNA copy number regardless of age. Further research is required to understand the role of AMH on mitochondria bioenergetics.

    DOI： 10.1186/s13048-022-01047-4

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    Other Link： https://link.springer.com/article/10.1186/s13048-022-01047-4/fulltext.html

  • Large-scale preparation of sialyl-Tn antigen-containing peptides from mucin-like glycoproteins in boar seminal gel Reviewed International journal

    Ryota Takeuchi, Megumi Maeda, Miran Nakano, Hiroaki Funahashi, Yoshinobu Kimura

    Bioscience biotechnology and biochemistry   85 ( 9 )   2022 - 2025   2021.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/bbb/zbab114

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  • The thickness and density of the ovarian tunica albuginea increases with age in transgender patients Reviewed International journal

    Pilar Ferre-Pujol, Junko Otsuki, Hiroaki Funahashi, Mikiya Nakatsuka

    Reproductive sciences   28 ( 5 )   1339 - 1346   2021.5

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s43032-020-00390-5

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  • Relative transcript abundance in porcine cumulus cells collected from different sized follicles Reviewed International coauthorship International journal

    Carla Moros-Nicolas, Ma Jose Izquierdo-Rico, Yang Li, Leopoldo Gonzalez-Brusi, Raquel Romar, Hiroaki Funahashi

    Reproduction in domestic animals   56 ( 2 )   374 - 380   2021.2

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/rda.13881

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  • Animal biotechnology roles in livestock production

    Hiroaki Funahashi

    International conference: Improving tropical animal production for food security   465 ( 1 )   2020

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (international conference proceedings)  

    DOI： 10.1088/1755-1315/465/1/012001

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  • The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles Reviewed International journal

    Chiyuki Kohata-Ono, Takuya Wakai, Hiroaki Funahashi

    Journal of reproduction and development   65 ( 6 )   527 - 532   2019.12

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1262/jrd.2019-112

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  • Removal of cumulus cells around 20 h after the start of in vitro maturation improves the meiotic competence of porcine oocytes via reduction in cAMP and cGMP levels Reviewed International coauthorship International journal

    Pilar Ferre-Pujol, Xuan Khanh Nguyen, Tomoki Nagahara, Thi Tra Mi Bui, Takuya Wakai, Hiroaki Funahashi

    Journal of reproduction and development   65 ( 2 )   177 - 182   2019.4

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1262/jrd.2018-130

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  • Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles Reviewed International journal

    Tra M. T. Bui, Khank X. Nguyen, Asako Karata, Pilar Ferre, Minh T. Tran, Takuya Wakai, Hiroaki Funahashi

    Reproduction fertility and development   29 ( 10 )   1902 - 1909   2017.9

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1071/RD16321

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  • Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles Reviewed International journal

    Tra M. T. Bui, Khank X. Nguyen, Asako Karata, Pilar Ferre, Minh T. Tran, Takuya Wakai, Hiroaki Funahashi

    REPRODUCTION FERTILITY AND DEVELOPMENT   29 ( 10 )   1902 - 1909   2017.9

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    DOI： 10.1071/RD16321

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  • Supplementation with cumulus cell masses improves the in vitro meiotic competence of porcine cumulus–oocytes complexes derived from small follicles Reviewed International journal

    R. Matsunaga, H. Funahashi

    Reproduction in domestic animals   52 ( 4 )   672 - 679   2017.8

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/rda.12967

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  • Levels of cyclic-AMP and cyclic-GMP in porcine oocyte-cumulus complexes and cumulus-free oocytes derived from small and middle follicles during the first 24-hour period of in vitro maturation Reviewed

    Yuichi Okudaira, Takuya Wakai, Hiroaki Funahashi

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   63 ( 2 )   191 - 197   2017.4

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1262/jrd.2016-156

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  • Effect of removing cumulus cells from porcine cumulus-oocyte complexes derived from small and medium follicles during IVM on the apoptotic status and meiotic progression of the oocytes Reviewed International journal

    Pilar Ferre, Tra Mi Thi Bui, Takuya Wakai, Hiroaki Funahashi

    THERIOGENOLOGY   86 ( 7 )   1705 - 1710   2016.10

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    DOI： 10.1016/j.theriogenology.2016.05.024

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  • Application of a microfluidic sperm sorter to in vitro production of dairy cattle sex-sorted embryos Reviewed International journal

    Jingchun Li, Sibing Zhu, Xianjing He, Rui Sun, Qianyu He, Yi Gan, Shengjun Liu, Hiroaki Funahashi, Yanbing Li

    THERIOGENOLOGY   85 ( 7 )   1211 - 1218   2016.4

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    DOI： 10.1016/j.theriogenology.2015.12.001

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  • Milk supplements in a glycerol free trehalose freezing extender enhanced cryosurvival of boar spermatozoa Reviewed

    Rukmali Athurupana, Hiroaki Funahashi

    Asian pacific journal of reproduction   5 ( 1 )   58 - 62   2016.3

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    DOI： 10.1016/j.apjr.2015.12.010

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  • A phosphodiesterase type-5 inhibitor, sildenafil, induces sperm capacitation and penetration into porcine oocytes in a chemically defined medium Reviewed International journal

    Sumire Ioki, Qing-Shan Wu, Osamu Takayama, Hideyuki H. Motohashi, Takuya Wakai, Hiroaki Funahashi

    THERIOGENOLOGY   85 ( 3 )   428 - 433   2016.2

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    DOI： 10.1016/j.theriogenology.2015.09.013

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  • In vitro fertilization in pigs: New molecules and protocols to consider in the forthcoming years Reviewed

    Raquel Romar, Hiroaki Funahashi, Pilar Coy

    THERIOGENOLOGY   85 ( 1 )   125 - 134   2016.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.theriogenology.2015.07.017

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  • Rapid thawing and stabilizing procedure improve postthaw survival and in vitro penetrability of boar spermatozoa cryopreserved with a glycerol-free trehalose-based extender Reviewed

    Rukmali Athurupana, Sumire Ioki, Hiroaki Funahashi

    THERIOGENOLOGY   84 ( 6 )   940 - 947   2015.10

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.theriogenology.2015.05.033

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  • Trehalose in glycerol-free freezing extender enhances post-thaw survival of boar spermatozoa Reviewed

    Rukmali Athurupana, Daisen Takahashi, Sumire Ioki, Hiroaki Funahashi

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   61 ( 3 )   205 - 210   2015.6

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1262/jrd.2014-152

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  • Development competence and relative transcript abundance of oocytes derived from small and medium follicles of prepubertal gilts Reviewed

    Chiyuki Kohata, Maria Jose Izquierdo-Rico, Raquel Romar, Hiroaki Funahashi

    THERIOGENOLOGY   80 ( 9 )   970 - 978   2013.12

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.theriogenology.2013.07.023

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  • A microfluidic device to reduce treatment time of intracytoplasmic sperm injection Reviewed International journal

    Koji Matsuura, Takuya Uozumi, Takuya Furuichi, Ikuyo Sugimoto, Mieko Kodama, Hiroaki Funahashi

    Fertility and sterility   99 ( 2 )   400 - 407   2013.2

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    DOI： 10.1016/j.fertnstert.2012.10.022

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  • Effects of caffeine on sperm characteristics after thawing and inflammatory response in the uterus after artificial insemination with frozen-thawed boar semen Reviewed International journal

    S. Yamaguchi, C. Suzuki, M. Noguchi, S. Kasa, M. Mori, Y. Isozaki, S. Ueda, H. Funahashi, K. Kikuchi, T. Nagai, K. Yoshioka

    Theriogenology   79 ( 1 )   87 - 93   2013.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.theriogenology.2012.09.012

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  • What is the optimal condition for fertilization of IVM oocytes? Invited Reviewed

    Hiroaki Funahashi

    Reproductive medicine and biology   12 ( 1 )   15 - 20   2013.1

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12522-012-0134-9

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  • Successful delivery derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia Reviewed International journal

    Yuji Endo, Yoshitaka Fujii, Shozo Kurotsuchi, Hiroaki Motoyama, Hiroaki Funahashi

    Fertility and Sterility   98 ( 6 )   1423 - 1427   2012.12

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    DOI： 10.1016/j.fertnstert.2012.07.1128

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  • Glycosaminoglycans Improves Early Development of Zona-free 8-cell Rat Embryos to Blastocysts in a Chemically Defined Medium, but Not the Pregnancy Rate Following Transfer of the Blastocysts Reviewed

    Masanobu Okuyama, Hiroaki Funahashi

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   58 ( 3 )   295 - 301   2012.6

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    DOI： 10.1262/jrd.11-092H

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  • Effect of the addition of beta-mercaptoethanol to a thawing solution supplemented with caffeine on the function of frozen-thawed boar sperm and on the fertility of sows after artificial insemination Reviewed International journal

    S. Yamaguchi, H. Funahashi

    Theriogenology   77 ( 5 )   926 - 932   2012.3

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    DOI： 10.1016/j.theriogenology.2011.09.023

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  • Simple vitrification for small numbers of human spermatozoa Reviewed International journal

    Yuji Endo, Yoshitaka Fujii, Kasumi Shintani, Momoyo Seo, Hiroaki Motoyama, Hiroaki Funahashi

    Reproductive BioMedicine Online   24 ( 3 )   301 - 307   2012.3

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    DOI： 10.1016/j.rbmo.2011.11.016

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  • Boar seminal plasma or hen's egg yolk decrease the in-vitro chemotactic and phagocytotic activities of neutrophils when co-incubated with boar or bull sperm Reviewed International journal

    J. C. Li, S. Yamaguchi, H. Funahashi

    Theriogenology   77 ( 1 )   73 - 80   2012.1

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    DOI： 10.1016/j.theriogenology.2011.07.018

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  • Caffeine, dibutyryl cyclic-AMP and heparin affect the chemotactic and phagocytotic activities of neutrophils for boar sperm in vitro Reviewed International journal

    J. C. Li, S. Yamaguchi, Y. Kondo, H. Funahashi

    Theriogenology   75 ( 7 )   1336 - 1345   2011.4

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    DOI： 10.1016/j.theriogenology.2010.12.005

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  • Single spermatozoon freezing using cryotop Reviewed

    Yuji Endo, Yoshitaka Fujii, Kasumi Shintani, Momoyo Seo, Hiroaki Motoyama, Hiroaki Funahashi

    Journal of Mammalian Ova Research   28 ( 1 )   47 - 52   2011

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  • Hydrophobic silicone elastomer chamber for recording trajectories of motile porcine sperms without adsorption Reviewed

    Koji Matsuura, Yuka Kuroda, Keisuke Yamashita, Hiroaki Funahashi

    Journal of Reproduction and Development   57 ( 1 )   163 - 167   2011

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    DOI： 10.1262/jrd.10-075N

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  • Internalization of REIC/Dkk-3 protein by induced pluripotent stem cell-derived embryoid bodies and extra-embryonic tissues Reviewed International journal

    Ken Kataoka, Masakiyo Sakaguchi, Kun Peng Li, Chika Taketa, Ken Ichi Yamamoto, Gang Du, Hiroaki Funahashi, Hitoshi Murata, Nam Ho Huh

    International Journal of Molecular Medicine   26 ( 6 )   853 - 859   2010.12

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    DOI： 10.3892/ijmm-00000534

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  • In-vitro Culture with a Tilting Device in Chemically Defined Media During Meiotic Maturation and Early Development Improves the Quality of Blastocysts Derived from In-vitro Matured and Fertilized Porcine Oocytes Reviewed

    Takayuki Koike, Koji Matsuura, Keiji Naruse, Hiroaki Funahashi

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   56 ( 5 )   552 - 557   2010.10

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    DOI： 10.1262/jrd.10-041H

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  • Effect of blood serum, caffeine and heparin on in vitro phagocytosis of frozen-thawed bull sperm by neutrophils derived from the peripheral blood of cows Reviewed International journal

    J. C. Li, H. Funahashi

    Theriogenology   74 ( 4 )   691 - 698   2010.9

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    DOI： 10.1016/j.theriogenology.2010.03.019

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  • Application of a microfluidic sperm sorter to the in-vitro fertilization of porcine oocytes reduced the incidence of polyspermic penetration Reviewed International journal

    Hikaru Sano, Koji Matsuura, Keiji Naruse, Hiroaki Funahashi

    THERIOGENOLOGY   74 ( 5 )   863 - 870   2010.9

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    DOI： 10.1016/j.theriogenology.2010.04.011

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  • Application of mechanical stimuli using a microfluidic air actuating system to cultured mammalian embryos Reviewed

    Jing Chun Li, Koji Matsuura, Yuka Kuroda, Hiroaki Funahashi, Keiji Naruse

    2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010   29 - 34   2010

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    DOI： 10.1109/MHS.2010.5669581

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  • Improved Fertility in Gilts and Sows after Artificial Insemination of Frozen-Thawed Boar Semen by Supplementation of Semen Extender with Caffeine and CaCl2 Reviewed

    Shoichiro Yamaguchi, Hiroaki Funahashi, Tetsuya Murakami

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   55 ( 6 )   645 - 649   2009.12

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    DOI： 10.1262/jrd.20238

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  • Successful Piglet Production in a Chemically Defined System for In-vitro Production of Porcine Embryos: Dibutyryl Cyclic AMP and Epidermal Growth Factor-family Peptides Support In-vitro Maturation of Oocytes in the Absence of Gonadotropins Reviewed

    Yuka Akaki, Koji Yoshioka, Michiko Noguchi, Hiroyoshi Hoshi, Hiroaki Funahashi

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   55 ( 4 )   446 - 453   2009.8

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    DOI： 10.1262/jrd.20219

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  • Exogenous adenosine Reduces the mitochondrial membrane potential of murine oocytes during the latter half of in vitro maturation and pronuclear formation following chemical activation Reviewed

    Wataru Fujii, Hiroaki Funahashi

    Journal of Reproduction and Development   55 ( 2 )   187 - 193   2009.5

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    DOI： 10.1262/jrd.20122

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  • Effect of glucose and pyruvate on nuclear and cytoplasmic maturation of porcine oocytes in a chemically defined medium Reviewed International journal

    H. Funahashi, T. Koike, R. Sakai

    Theriogenology   70 ( 7 )   1041 - 1047   2008.10

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    DOI： 10.1016/j.theriogenology.2008.06.025

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  • Metal mesh vitrification (MMV) method for cryopreservation of porcine embryos Reviewed

    Y. Fujino, T. Kojima, Y. Nakamura, H. Kobayashi, K. Kikuchi, H. Funahashi

    Theriogenology   70 ( 5 )   809 - 817   2008.9

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    DOI： 10.1016/j.theriogenology.2008.05.045

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  • In vitro development of non-enucleated rat oocytes following microinjection of a cumulus nucleus and chemical activation Reviewed International journal

    Wataru Fujii, Hiroaki Funahashi

    Zygote   16 ( 2 )   117 - 125   2008.5

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    DOI： 10.1017/S0967199408004632

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  • Up date of in vitro production of porcine embryos Invited Reviewed International journal

    Takashi Nagai, Hiroaki Funahashi, Koji Yoshioka, Kazuhiro Kikuchi

    Frontiers in bioscience-landmark   11 ( SUPPL. 2 )   2565 - U34   2006.9

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    DOI： 10.2741/1991

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  • In vitro maturation and fertilization of porcine oocytes after a 48 h culture in roscovitine, an inhibitor of p34(cdc2)/cyclin B kinase Reviewed International journal

    R Romar, H Funahashi

    ANIMAL REPRODUCTION SCIENCE   92 ( 3-4 )   321 - 333   2006.5

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    DOI： 10.1016/j.anireprosci.2005.04.017

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  • Effect of beta-mercaptoethanol during in vitro fertilization procedures on sperm penetration into porcine oocytes and the early development in vitro. Reviewed International journal

    Hiroaki Funahashi

    Reproduction (cambridge, england)   130 ( 6 )   889 - 98   2005.12

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    DOI： 10.1530/rep.1.00702

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  • Select antioxidants improve the function of extended boar semen stored at 10°C Reviewed

    H. Funahashi, T. Sano

    Theriogenology   63 ( 6 )   1605 - 1616   2005.4

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    DOI： 10.1016/j.theriogenology.2004.06.016

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  • Reduction of the incidence of polyspermic penetration into porcine oocytes by pretreatment of fresh spermatozoa with adenosine and a transient co-incubation of the gametes with caffeine Reviewed

    Hiroaki Funahashi, Raquel Romar

    Reproduction   128 ( 6 )   789 - 800   2004.12

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    To reduce the incidence of polyspermic penetration, the effects of transient exposure of washed fresh spermatozoa to caffeine in a brief co-culturein vitrofertilization (IVF) system were examined. A pretreatment effect of spermatozoa with adenosine was also examined. When 5 mmol caffeine/l was supplemented during periods of co-culture and additional culture periods until 8 h after insemination, a shortened co-incubation period of gametes (30 denuded oocytes in 100 μl modified Medium 199-suspended spermatozoa at 2.5 ×105sperm/ml) from 30 to 5 min increased the monospermy rate in total mature oocytes examined. The number of spermatozoa binding to the zona surface was significantly lower in oocytes co-cultured for 5 min (33.1 ± 2.2) than 8 h (207.6 ± 13.7). A limited exposure of gametes to 5 mmol caffeine/l only during a transient co-culture period for 5 or 30 min significantly reduced the mean number of sperm cells that penetrated into the oocyte. Transient exposure of spermatozoa to caffeine for only 5 min increased the percentage of capacitated cells but not acrosome-reacted cells, as compared with a whole exposure treatment. Furthermore, preincubation of spermatozoa with 10 μmol adenosine/l for 90 min increased both the incidence of capacitated cells and the monospermy rate and consequently decreased the number of sperm cells that penetrated into the oocyte. In conclusion, these results have demonstrated that a new transient co-incubation IVF system, in which denuded oocytes are co-cultured with spermatozoa in medium containing caffeine for 5 to 30 min and then continuing the culture in caffeine-free medium, will reduce the incidence of polyspermic penetration. Preincubation of fresh spermatozoa with adenosine before the transient co-incubation IVF can also improve the monospermy rate. Furthermore, asynchrony in the morphology of sperm nuclei in polyspermic oocytes was reduced by the pretreatment with adenosine and a brief exposure to caffeine.

    DOI： 10.1530/rep.1.00295

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  • Polyspermic penetration in porcine IVM - IVF systems Reviewed

    Hiroaki Funahashi

    Reproduction, Fertility and Development   15 ( 3 )   167 - 167   2003

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    Although techniques for in vitro production of porcine embryos have proceeded very rapidly during the past decade, polyspermic penetration still remains a persistent obstacle to porcine in vitro fertilization (IVF) systems. Considerable research on in vitro polyspermic penetration in porcine in vitro-matured (IVM) oocytes has been undertaken to try to solve this problem. In the current paper, recent advancements in overcoming the problems of polyspermy in porcine IVF systems are reviewed. Partial induction of the acrosome reaction of boar spermatozoa in IVF media that contain caffeine is likely to be one of the major causes of polyspermy. A reduction in the number of incompletely acrosome-reacted spermatozoa, which can bind tightly to the zona pellucida and mask free sperm receptors of the zona pellucida, could reduce the incidence of polyspermic penetration; however, morphological differences in the reaction of the zona pellucida have been observed between IVM and ovulated oocytes, which suggests that altered zona morphology may be another cause of polyspermic penetration. It has been shown that the developmental ability of polyspermic porcine embryos to the blastocyst stage is similar to that of normal embryos but that developmental competence to term is much lower. To overcome the current problems of polyspermy, it is suggested that future efforts should be focused on controlling boar sperm function and/or sperm–zona binding to achieve the final maturation associated with normal zona modifications of porcine oocytes at fertilization.

    DOI： 10.1071/rd02076

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  • Induction of capacitation and the acrosome reaction of boar spermatozoa by L-arginine and nitric oxide synthesis associated with the anion transport system Reviewed

    H Funahashi

    Reproduction   857 - 864   2002.12

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    The aim of this study was to determine the effect of L-arginine on nitric oxide (NO) synthesis, capacitation and acrosome reaction of boar spermatozoa. Ejaculated boar spermatozoa were washed and then cultured in a bicarbonate:CO(2)-buffered medium, modified NCSU-37, for 2 h. At the end of the culture, the status of spermatozoa was determined. The presence of (0.1-2.0 mmol l(-1)) L-arginine in the culture medium induced an acrosome reaction as determined by fluorescein isothiocyanate-peanut agglutinin (FITC-PNA) and increased intracellular NO content, as quantified by a fluorescent indicator, diaminofluorescein-2 diacetate (DAF-2 DA). This stimulatory effect of L-arginine was neutralized by supplementation with an NO synthase inhibitor, N(omega)-nitro-L-arginine methyl ester (1 mmol l(-1)). However, the inactive enantiomorph, N(omega)-nitro-D-arginine methyl ester, did not affect the stimulatory effect of L-arginine. These results indicate that L-arginine induces an acrosome reaction through the NO signal pathway in boar spermatozoa. Furthermore, the stimulatory effect of L-arginine was inhibited in the presence of an anion transport inhibitor, 4-acetamido-4'-isothiocyano-stilbene-2,2'-disulphonic acid (SITS; 0.1 mmol l(-1)), whereas any responses of spermatozoa to caffeine were not inhibited by SITS. A stimulatory effect of L-arginine on capacitation and acrosome reaction of spermatozoa was also observed in modified NCSU37 medium by using a chlortetracycline fluorescence assay, but not in supplemented bicarbonate-free Tris-buffered medium. These results indicate that the presence of L-arginine induces nitric oxide synthesis and stimulates capacitation and acrosome reaction of boar spermatozoa only when active sperm anion transport is present as a result of bicarbonate supplementation.

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  • Effect of Methyl-.BETA.-Cyclodextrin and Fertilization Promoting Peptide on Capacitation of Boar Spermatozoa in a Protein-Free Medium. Reviewed

    Hiroaki FUNAHASHI

    Journal of Reproduction and Development   48 ( 1 )   57 - 63   2002

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    DOI： 10.1262/jrd.48.57

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  • Nuclear Transfer of Blastomeres Expressing EGFP-Reporter Gene May Improve the Efficiency of Transgenic Cattle Reviewed

    H. Funahashi, A. Ideta, M. Konishi, M. Urakawa, K. Uruno, Y. Aoyagi, M. Okabe, K. Niwa

    Cloning and Stem Cells   3 ( 4 )   183 - 190   2001.12

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    DOI： 10.1089/15362300152725891

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  • Transmission electron microscopy studies of the zona reaction in pig oocytes fertilized in vivo and in vitro Reviewed

    H Funahashi, H Ekwall, K Kikuchi, H Rodriguez-Martinez

    Reproduction   443 - 452   2001.9

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    The aim of this study was to determine the ultrastructure of cross-sectioned zonae pellucidae of in vitro-matured and ovulated pig oocytes before or after sperm penetration in vitro and in vivo, respectively. The in vitro and in vivo (ovulated) oocytes and zygotes (fertilized in vitro and in vivo) were fixed with glutaraldehyde either directly or after pretreatment with ruthenium red and saponin, processed and then examined using transmission electron microscopy. The thickness of the zona pellucida, as measured on the section of the specimens with largest diameter fixed with glutaraldehyde, differed between the in vivo (9.19 +/- 0.47 microm) and in vitro (5.95 +/- 0.51 microm) oocytes. The in vivo oocytes had a rather thick external mesh-like structure, whereas it was much thinner in the in vitro oocytes. This mesh-like external rim was less apparent in both in vivo and in vitro zygotes. Obvious differences in the density of the lattice formed by the fixed zonae pellucidae were visible between the outer and inner (ad-oolemmal) zonae. The outer area always formed a concentrically arrayed fibrillar network, whereas the inner area showed a much more compact, trabecule-like mesh. However, both areas, but particularly the outer network, were much more compacted after the zona reaction. Clear differences in the degree of fibrillar aggregation of the inner zona area were also observed between in vitro and in vivo zygotes, being much higher in the latter. This fibrillar network was more clearly visible in the zygotes pretreated with ruthenium red and saponin; the in vitro zygotes had a fibrillar, radially oriented set of parallel fibrils, whereas it was much more aggregated and trabecule-like in the in vivo zygotes. These results demonstrate that the fine structure of the zona pellucida and the zona reaction at sperm penetration differ between pig oocytes fertilized in vivo and in vitro. Moreover, the ultrastructure of the outer and inner pig zonae pellucidae has a different network organization.

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  • Regulation of in vitro penetration of frozen-thawed boar spermatozoa by caffeine and adenosine Reviewed

    Hiroaki Funahashi, Takashi Nagai

    Molecular Reproduction and Development   58 ( 4 )   424 - 431   2001.4

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    DOI： 10.1002/1098-2795(20010401)58:4<424::aid-mrd10>3.0.co;2-1

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  • Involvement of oviduct in sperm capacitation and oocyte development in pigs Invited Reviewed

    H Rodriguez-Martinez, P Tienthai, K Suzuki, H Funahashi, H Ekwall, A Johannisson

    CONTROL OF PIG REPRODUCTION VI   58 ( 58 )   129 - 145   2001

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  • Zona Reaction in Porcine Oocytes Fertilized In Vivo and In Vitro as Seen with Scanning Electron Microscopy1 Reviewed

    Hiroaki Funahashi, Hans Ekwall, Heriberto Rodriguez-Martinez

    Biology of Reproduction   63 ( 5 )   1437 - 1442   2000.11

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    DOI： 10.1095/biolreprod63.5.1437

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  • Modulation of the Function of Boar Spermatozoa via Adenosine and Fertilization Promoting Peptide Receptors Reduce the Incidence of Polyspermic Penetration into Porcine Oocytes1 Reviewed

    Hiroaki Funahashi, Toshimitsu Fujiwara, Takashi Nagai

    Biology of Reproduction   63 ( 4 )   1157 - 1163   2000.10

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    DOI： 10.1095/biolreprod63.4.1157

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  • Both fertilization promoting peptide and adenosine stimulate capacitation but inhibit spontaneous acrosome loss in ejaculated boar spermatozoa in vitro Reviewed

    Hiroaki Funahashi, Atsushi Asano, Toshimitsu Fujiwara, Takashi Nagai, Koji Niwa, Lynn R. Fraser

    Molecular Reproduction and Development   55 ( 1 )   117 - 124   2000.1

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    DOI： 10.1002/(sici)1098-2795(200001)55:1<117::aid-mrd16>3.0.co;2-7

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  • Sperm Selection by a Climbing-over-a-Wall IVF Method Reduces the Incidence of Polyspermic Penetration of Porcine Oocytes. Reviewed

    Hiroaki FUNAHASHI, Takashi NAGAI

    Journal of Reproduction and Development   46 ( 5 )   319 - 324   2000

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    DOI： 10.1262/jrd.46.319

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  • Changes in intracellular content of glutathione and thiols associated with γ-glutamyl cycle during sperm penetration and pronuclear formation in rat oocytes Reviewed

    Hiroaki Funahashi, Naoya Bandoh, Shinobu Nakahira, She-Hoon Oh, Seiji Tsuboi

    Zygote   7 ( 4 )   301 - 305   1999.11

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    The content of glutathione and other thiols in rat eggs was examined during sperm penetration and pronuclear formation by high-performance liquid chromatography with fluorescence detection. Reduced glutathione (GSH) content was higher in unfertilised oocytes (8.50 ± 0.29 pmol/egg) and penetrated eggs with a decondensed sperm nucleus (DSH eggs; 7.72 ± 0.56 pmol/egg) than eggs at the pronuclear stage (PN eggs; 5.93 ± 0.10 pmol/egg). The content of oxidised glutathione (GSSG) was not different among experimental groups (152.6 ± 74.1 nmol/egg in unfertilised eggs, 146.0 ± 50.0 nmol/egg in DSH eggs and 39.7 ± 17.3 nmol/egg in PN eggs). The GSSG/GSH ratio did not change during fertilisation. Although the reduced cysteinylglycine content of eggs did not change among experimental groups, the oxidised form of cysteinylglycine increased (p &lt; 0.025) between sperm decondensation (6.9 ± 1.5 nmol/egg in unfertilised oocytes and 10.1 ± 2.1 nmol/egg in DSH eggs) and pronuclear formation (40.5 ± 11.5 nmol/egg in PN eggs). Low contents of cystine were detected during fertilisation but cysteine and γ-glutamylcysteine were not detected in any treatment groups. These results demonstrate that GSH content in rat eggs decreases between sperm decondensation and pronuclear formation, probably due to the increased activity of γ-glutamyl transpeptidase.

    DOI： 10.1017/s0967199499000702

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  • DNA stability and thiol-disulphide status of rat sperm nuclei during epididymal maturation and penetration of oocytes Reviewed

    Syahruddin Said, Hiroaki Funahashi, Koji Niwa

    Zygote   7 ( 3 )   249 - 254   1999.8

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    DNA stability and thiol-disulphide status of rat sperm nuclei was observed in vivo during maturation in the epididymis and penetration of oocytes. When spermatids and spermatozoa were stained with acridine orange after fixation with acetic alcohol, the red/green fluorescence ratio observed under a confocal microscope was not different between spermatids (3.81 ± 0.16) and testicular spermatozoa (4.03 ± 0.34), and then decreased sharply (p &lt; 0.01) as the spermatozoa descended the epidymis to the caput epididymis (1.13 ± 0.03). However, the ratio was not different among corpus (0.69 ± 0.01), cauda epididymis (0.68 ± 0.11) and ejaculated spermatozoa (0.63 ± 0.01). On the other hand, when spermatozoa were labelled with monobromobimane (mBBr), the fluorescence intensities gradually decreased (p &lt; 0.01) during passage of spermatozoa from testis (4.74 ± 0.16) through epididymis (caput, 2.72 ± 0.08; corpus, 1.07 ± 0.03; cauda, −0.05 ± 0.05; ejaculated, 0.08 ± 0.03). The acridine orange red/green fluorescence ratio increased (p &lt; 0.01) during zona penetration (binding sperm, 0.52 ± 0.09; perivitelline sperm, 0.64 ± 0.16) and sperm decondensation (decondensed sperm, 0.69 ± 0.12). When spermatozoa in the perivitelline space were labelled with mBBr, the fluorescence was detected. These results demonstrate that DNA stability against acid appears to be ahead of the oxidation of protamine during sperm maturation in the epididymis and is an initial event of the unpackaging process in rat genome occurring during or just after zona penetration but before ooplasm penetration.

    DOI： 10.1017/s0967199499000635

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  • Production of Plasminogen Activators (PAs) in Bovine Cumulus-Oocyte Complexes during Maturation In Vitro: Effects of Epidermal Growth Factor on Production of PAs in Oocytes and Cumulus Cells1 Reviewed

    Kwang-Wook Park, Sun-Ho Choi, Xue-Xiong Song, Hiroaki Funahashi, Koji Niwa

    Biology of Reproduction   61 ( 1 )   298 - 304   1999.7

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    DOI： 10.1095/biolreprod61.1.298

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  • Co-culture of Cumulus-Enclosed Bovine Oocytes with Theca Cells Induces the Meiotic Arrest but does not Inhibit Germinal Vesicle Development. Reviewed

    Kwang-Wook Park, Hiroaki Funahashi, Koji Niwa

    Journal of Reproduction and Development   45 ( 3 )   223 - 231   1999

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    DOI： 10.1262/jrd.45.223

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  • The Presence of Tissue Inhibitor of Matrix Metalloproteinase-1 (TIMP-1) During Meiosis Improves Porcine 'Oocyte Competence' as Determined by Early Embryonic Development After In-vitro Fertilization. Reviewed

    Hiroaki Funahashi, Eric W. McIntush, Michael F. Smith, Billy N. Day

    Journal of Reproduction and Development   45 ( 4 )   265 - 271   1999

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Animal Reproduction  

    DOI： 10.1262/jrd.45.265

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  • Rat Oocytes Fertilized in Modified Rat 1-Cell Embryo Culture Medium Containing a High Sodium Chloride Concentration and Bovine Serum Albumin Maintain Developmental Ability to the Blastocyst Stage Reviewed

    She-Hoon Oh, Kazuchika Miyoshi, Hiroaki Funahashi

    Biology of Reproduction   59 ( 4 )   884 - 889   1998.10

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1095/biolreprod59.4.884

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  • Effects of Cumulus Cells on the Ability of Pig Oocytes to Utilize Cysteine or Cystine During Maturation In Vitro. Reviewed

    Ken SAWAI, Hiroaki FUNAHASHI, Koji NIWA

    Journal of Reproduction and Development   44 ( 2 )   161 - 168   1998

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    DOI： 10.1262/jrd.44.161

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  • Stage-Specific Requirement of Cysteine during in Vitro Maturation of Porcine Oocytes for Glutathione Synthesis Associated with Male Pronuclear Formation1 Reviewed

    Ken Sawai, Hiroaki Funahashi, Koji Niwa

    Biology of Reproduction   57 ( 1 )   1 - 6   1997.7

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1095/biolreprod57.1.1

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  • Synchronization of Meiosis in Porcine Oocytes by Exposure to Dibutyryl Cyclic Adenosine Monophosphate Improves Developmental Competence Following in Vitro Fertilization1 Reviewed

    Hiroaki Funahashi, Thomas C. Cantley, Billy N. Day

    Biology of Reproduction   57 ( 1 )   49 - 53   1997.7

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1095/biolreprod57.1.49

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  • Chlortetracycline fluorescence patterns andin vitrofertilisation of frozen-thawed boar spermatozoa incubated under various bicarbonate concentrations Reviewed

    Lalantha R. Abeydeera, Hiroaki Funahashi, Nam-Hyung Kim, Billy N. Day

    Zygote   5 ( 2 )   117 - 125   1997.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Cambridge University Press (CUP)  

    Summary

    Porcine oocyte-cumulus complexes were cultured in bovine serum albumin(BSA)-free North Carolina State University (NCSU)23 medium containing porcine follicular fluid (10%), cysteine (0.1 mg/ml)and hormonal supplements (eCG and hCG: 10IU/ml each) for 22h. They were then cultured in the same medium but without hormonal supplements for an additional 22h. After culture, cumulus cells were removed and oocytes were co-incubated with frozen-thawed ejaculated boar spermatozoa in tissue culture medium (TCM)199 containing caffeine (5mM), fetal calf serum (FCS; 10%) and varying concentrations (26–56 mM)of NaHCO3for 9h(experiment 1). In experiment 2, chlortetracycline (CTC) was used to assess the functional state of spermatozoa incubated under different NaHCO3concentrations. Experiment 3 examined the effect of FCS (1% and 10%)and NaHCO3(26 and 46 mM) on fertilisation parameters. Compared with 26 mM, penetration rate was significantly higher (p&lt;0.05)at 36–56mM NaHCO3. Polyspermy showed a similar pattern although no difference was observed between 26 and 36 mM. At 46mM NaHCO3, the mean number of spermatozoa (MNS) penetrated per oocyte increased significantly (p&lt; 0.05). A significantly higher proportion of spermatozoa were capacitated and acrosome reacted at 46 and 56mM NaHCO3, respectively. The fertilisation medium containing 46mM NaHCO3and 1% FCS showed a higher penetration rate (84%)with a relatively low incidence of polyspermy (39%). The results indicate that NaHCO3stimulates capacitation and/or the acrosome reaction of boar spermatozoa in a dose-dependent manner and thus affects fertilisation parameters.

    DOI： 10.1017/s0967199400003798

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  • Developmental Changes in the Intracellular Ca 2+ Release Mechanisms in Porcine Oocytes1 Reviewed

    Zoltán Macháty, Hiroaki Funahashi, Billy N. Day, Randall S. Prather

    Biology of Reproduction   56 ( 4 )   921 - 930   1997.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1095/biolreprod56.4.921

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  • Preincubation of cumulus-oocyte complexes before exposure to gonadotropins improves the developmental competence of porcine embryos matured and fertilized in vitro Reviewed International journal

    H Funahashi, TC Cantley, BN Day

    THERIOGENOLOGY   47 ( 3 )   679 - 686   1997.2

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Web of Science

    PubMed

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  • Effects of Cysteine in Serum-Free Maturation Medium on Male Pronuclear Formation of Maturing Pig Oocytes Penetrated In Vitro. Reviewed

    Ken SAWAI, Hiroaki FUNAHASHI, Wei-Hua WANG, Koji NIWA

    Journal of Reproduction and Development   43 ( 1 )   73 - 80   1997

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Animal Reproduction  

    DOI： 10.1262/jrd.43.73

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  • gamma-glutamyl transpeptidase of spermatozoa may decrease oocyte glutathione content at fertilization in pigs Reviewed International journal

    H Funahashi, Z Machaty, RS Prather, BN Day

    MOLECULAR REPRODUCTION AND DEVELOPMENT   45 ( 4 )   485 - 490   1996.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Web of Science

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  • γ-glutamyl transpeptidase of spermatozoa may decrease oocyte glutathione content at fertilization in pigs Reviewed

    Hiroaki Funahashi, Zoltan Machaty, Randall S. Prather, Billy N. Day

    Molecular Reproduction and Development   45 ( 4 )   485 - 490   1996.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1002/(sici)1098-2795(199612)45:4<485::aid-mrd11>3.0.co;2-w

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  • Microtubule Organization in Porcine Oocytes during Fertilization and Parthenogenesis1 Reviewed

    Nam-Hyung Kim, Calvin Simerly, Hiroaki Funahashi, Gerald Schatten, Billy N. Day

    Biology of Reproduction   54 ( 6 )   1397 - 1404   1996.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1095/biolreprod54.6.1397

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  • Presence of Organic Osmolytes in Maturation Medium Enhances Cytoplasmic Maturation of Porcine Oocytes1 Reviewed

    Hiroaki Funahashi, Nam-Hyung Kim, Todd T. Stumpf, Thomas C. Cantley, Billy N. Day

    Biology of Reproduction   54 ( 6 )   1412 - 1419   1996.6

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1095/biolreprod54.6.1412

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  • Effects of oviductal fluid on sperm penetration and cortical granule exocytosis during fertilization of pig oocytes in vitro Reviewed

    N-H. Kim, H. Funahashi, L. R. Abeydeera, S. J. Moon, R. S. Prather, B. N. Day

    Reproduction   107 ( 1 )   79 - 86   1996.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Bioscientifica  

    DOI： 10.1530/jrf.0.1070079

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  • Effects of Injecting Calcium Chloride into in Vitro-Matured Porcine Oocytes1 Reviewed

    Z. Macháty, H. Funahashi, M. A. Mayes, B. N. Day, R. S. Prather

    Biology of Reproduction   54 ( 2 )   316 - 322   1996.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1095/biolreprod54.2.316

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  • Microtubule and microfilament dynamics in porcine oocytes during meiotic maturation Reviewed

    Nam‐Hyung Kim, Hiroaki Funahashi, Randall S. Prather, Gerald Schatten, Billy N. Day

    Molecular Reproduction and Development   43 ( 2 )   248 - 255   1996.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1002/(sici)1098-2795(199602)43:2<248::aid-mrd14>3.0.co;2-#

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  • Current status of in vitro production of porcine embryos Invited Reviewed

    H Funahashi, BN Day

    ADVANCES IN SWINE IN BIOMEDICAL RESEARCH, VOLS 1 AND 2   491 - 502   1996

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    Authorship：Lead author   Language：English   Publishing type：Research paper (international conference proceedings)  

    Web of Science

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  • Factors Affecting Development In Vitro of Bovine and Rat 1-Cell Embryos. Reviewed

    Kazuchika Miyoshi, Hiroaki Funahashi, Koji Niwa

    Journal of Mammalian Ova Research   13 ( 2 )   71 - 80   1996

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Mammalian Ova Research  

    DOI： 10.1274/jmor.13.71

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  • Low Salt Maturation Medium Enhances the Histone H1 Kinase Activity of Porcine Oocytes at the End of In Vitro Maturation. Reviewed

    Hiroaki FUNAHASHI, Todd T. STUMPF, Nam-Hyung KIM, Billy N. DAY

    Journal of Reproduction and Development   42 ( 2 )   109 - 115   1996

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Animal Reproduction  

    DOI： 10.1262/jrd.42.109

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  • Pronuclear formation and intracellular glutathione content ofin vitro-matured porcine oocytes followingin vitrofertilisation and/or electrical activation Reviewed

    Hiroaki Funahashi, Todd T. Stumpf, Thomas C. Cantley, Nam-Hyung Kim, Billy N. Day

    Zygote   3 ( 3 )   273 - 281   1995.8

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Cambridge University Press (CUP)  

    Summary

    Pronuclear formation and intracellular content of glutathione, containing reduced and oxidised forms, in porcine oocytes maturedin vitrowere determined following insemination and/or electrical stimulation. After insemination, sperm penetration had occurred as early as 3 h and female pronuclei had formed by 6 h with complete development by 12 h. Male pronuclear formation occurred, primarily, between 9 and 12 h after insemination. Glutathione content of the oocytes decreased following sperm penetration and remained at a depressed level until 12 h. After electrical stimulation, oocyte activation had occurred and female pronuclei had formed by 3 and 6 h, respectively. Oocyte glutathione content did not change as a result of oocyte activation. When oocytes were exposed to an electrical pulse and then spermatozoa, female pronuclear formation was observed by 3 h after stimulation/insemination. Sperm penetration was observed between 3 and 9 h. However, the incidence of male pronuclear formation observed at 12 h was extremely low, although sperm decondensation had occurred in some oocytes. Oocyte glutathione content had not decreased by 6 h following electrical activation. These results demonstrate that the changes in glutathione content in porcine oocytes following fertilisationin vitrodiffer from those due to electrical activation. Further, the decreased intracellular glutathione content in oocytes activated by sperm penetration appears to be due to the presence of a sperm factor.

    DOI： 10.1017/s0967199400002677

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  • Pronuclear visibility, development and transgene expression in IVM/IVF-derived porcine embryos Reviewed

    H.-M. Kubisch, M.A. Larson, H. Funahashi, B.N. Day, R.M. Roberts

    Theriogenology   44 ( 3 )   391 - 401   1995.8

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/0093-691x(95)00193-c

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  • Use of Low-Salt Culture Medium for in Vitro Maturation of Porcine Oocytes is Associated with Elevated Oocyte Glutathione Levels and Enhanced Male Pronuclear Formation after in Vitro Fertilization1 Reviewed

    Hiroaki Funahashi, Thomas C. Cantley, Todd T. Stumpf, Steven L. Terlouw, Billy N. Day

    Biology of Reproduction   51 ( 4 )   633 - 639   1994.10

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1095/biolreprod51.4.633

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  • In Vitro Development of in Vitro-Matured Porcine Oocytes Following Chemical Activation or in Vitro Fertilization1 Reviewed

    Hiroaki Funahashi, Thomas C. Cantley, Todd T. Stumpf, Steven L. Terlouw, Billy N. Day

    Biology of Reproduction   50 ( 5 )   1072 - 1077   1994.5

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1095/biolreprod50.5.1072

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  • Different hormonal requirements of pig oocyte-cumulus complexes during maturation in vitro Reviewed

    H. Funahashi, T. Cantley, B. N. Day

    Reproduction   101 ( 1 )   159 - 165   1994.5

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Bioscientifica  

    DOI： 10.1530/jrf.0.1010159

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  • Development of rat one-cell embryos in a chemically defined medium: effects of glucose, phosphate and osmolarity Reviewed

    K. Miyoshi, H. Funahashi, K. Okuda, K. Niwa

    Reproduction   100 ( 1 )   21 - 26   1994.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Bioscientifica  

    DOI： 10.1530/jrf.0.1000021

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  • Developmental ability of porcine oocytes matured and fertilized in vitro Reviewed

    H. Funahashi, T.T. Stumpf, S.L. Terlouw, T.C. Cantley, A. Rieke, B.N. Day

    Theriogenology   41 ( 7 )   1425 - 1433   1994.1

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/0093-691x(94)90193-m

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  • Effects of electrical stimulation before or after in vitro fertilization on sperm penetration and pronuclear formation of pig oocytes Reviewed

    Hiroaki Funahashi, Todd T. Stumpf, Steve L. Terlouw, Billy N. Day

    Molecular Reproduction and Development   36 ( 3 )   361 - 367   1993.11

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1002/mrd.1080360312

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  • Effects of follicular fluid at fertilization in vitro on sperm penetration in pig oocytes Reviewed

    H. Funahashi, B. N. Day

    Reproduction   99 ( 1 )   97 - 103   1993.9

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Bioscientifica  

    DOI： 10.1530/jrf.0.0990097

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  • Effects of the duration of exposure to hormone supplements on cytoplasmic maturation of pig oocytes in vitro Reviewed

    H. Funahashi, B. N. Day

    Reproduction   98 ( 1 )   179 - 185   1993.5

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Bioscientifica  

    DOI： 10.1530/jrf.0.0980179

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  • Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium Reviewed

    J.H. Kim, H. Funahashi, K. Niwa, K. Okuda

    Theriogenology   39 ( 4 )   875 - 886   1993.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/0093-691x(93)90425-5

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  • Effects of different serum supplements in maturation medium on meiotic and cytoplasmic maturation of pig oocytes Reviewed

    H. Funahashi, B.N. Day

    Theriogenology   39 ( 4 )   965 - 973   1993.4

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/0093-691x(93)90433-6

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  • Fertilization and early cleavage in vitro of ageing bovine oocytes after maturation in culture Reviewed

    R.C. Chian, H. Nakahara, K. Niwa, H. Funahashi

    Theriogenology   37 ( 3 )   665 - 672   1992.3

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/0093-691x(92)90146-i

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  • Developmental Abilities of Rat and Mouse Pronuclear Eggs Following Interspecific Nuclear or Cytoplasmic Transplantation. Reviewed

    Hiroaki FUNAHASHI, Koji NIWA

    Journal of Reproduction and Development   38 ( 3 )   179 - 183   1992

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Animal Reproduction  

    DOI： 10.1262/jrd.38.179

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  • First cleavage of enucleated rat eggs following transplantation of karyoplast removed from pronuclear eggs stored at 2 to 6°C for various durations Reviewed

    K. Niwa, H. Funahashi, K. Ohmae, M. Kattoh

    Theriogenology   36 ( 3 )   411 - 417   1991.9

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/0093-691x(91)90469-t

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  • Developmental capacity of bovine oocytes collected from ovaries of individual heifers and fertilized in vitro Reviewed

    H. Funahashi, Y. Aoyagi, T. Takeda, T. Onihara

    Theriogenology   36 ( 3 )   427 - 434   1991.9

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/0093-691x(91)90471-o

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Books

  • Assisted Reproductive Technologies in Animals Volume 1: Current Trends for Reproductive Management Reviewed International journal

    Funahashi, H., Nguyen, H.T., Wakai, T.（ Role： Contributor ,  Sperm mitochondria: quantitative regulation and its impact on sperm quality）

    Springer Nature  2025.2  （ ISBN:303173078X ）

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    Total pages：523   Responsible for pages：18   Language：English Book type：Scholarly book

    DOI： 10.1007/978-3-031-73079-5

    ASIN

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  • 生殖補助医療技術学入門

    岡山大学生殖補助医療学教科書作成委員会（ Role： Contributor ,  第Ⅰ章 生殖補助医療の歴史と現状）

    岡山大学出版会  2017.9  （ ISBN:9784904228555 ）

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    Total pages：126p   Language：Japanese

    CiNii Books

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  • 生殖補助医療技術学入門

    岡山大学生殖補助医療学教科書作成委員会（ Role： Contributor ,  第Ⅳ章 関係基礎技術）

    岡山大学出版会  2017.9  （ ISBN:9784904228555 ）

    　More details

    Total pages：126p   Language：Japanese

    CiNii Books

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  • 生殖補助医療技術学入門

    岡山大学生殖補助医療学教科書作成委員会（ Role： Contributor ,  第Ⅲ章 卵母細胞と精子の基礎）

    岡山大学出版会  2017.9  （ ISBN:9784904228555 ）

    　More details

    Total pages：126p   Language：Japanese

    CiNii Books

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  • 基礎演習産業生物科学

    産業生物科学教科書編纂委員会（ Role： Contributor ,  クローン動物・細胞周期・体細胞分裂と減数分裂・有性生殖の戦略）

    岡山大学出版会  2009.9  （ ISBN:9784904228050 ）

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    Total pages：x, 314p   Language：Japanese

    CiNii Books

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  • 瞬時共培養法を用いた新たな豚体外受精系の構築

    舟橋, 弘晃

    舟橋弘晃  2006.3 

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    Total pages：58p   Language：English

    CiNii Books

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  • 基礎のためのIVM．エンブリオロジストのためのART必須ラボマニュアル（日本臨床エンブリオロジスト研究会編，監修：鈴木秋悦・福田愛作

    医歯薬出版  2005 

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  • 緑色蛍光蛋白質(GFP)を利用した形質転換動物の効率的システムの開発

    舟橋, 弘晃

    舟橋弘晃  2002.3 

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    Total pages：108p  

    CiNii Books

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  • Oviduct involvement in sperm capacitation and oocyte development.

    Control of Pig Reproduction VI.(ISBN 0-906545-32-3), Eds.Foxcroft, G.R., Geisert, R.D.and Doberska, C.Journal of Reproduction and Reproduction and Fertility Ltd.,Cambridge,UK  2001 

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  • Oviduct involvement in sperm capacitation and oocyte development.

    Control of Pig Reproduction VI.(ISBN 0-906545-32-3), Eds.Foxcroft, G.R., Geisert, R.D.and Doberska, C.Journal of Reproduction and Reproduction and Fertility Ltd.,Cambridge,UK  2001 

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  • Polyspermic penetration in porcine IVF systems;why so frequent?

    Reproductive Biotechnology:Reproductive Biotechnilogy Update and its Related Physiology, Eds.  2001 

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  • Polyspermic penetration in porcine IVF systems;why so frequent?

    Reproductive Biotechnology:Reproductive Biotechnilogy Update and its Related Physiology, Eds.  2001 

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  • 哺乳動物雄性ゲノムのプロタミン封入および雄性前核形成の調節機構の解析

    舟橋, 弘晃

    舟橋弘晃  1999.3 

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    Total pages：60p  

    CiNii Books

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  • Factors affecting the efficiency of in-vitro production of porcine embryos. "Reproductive Biology update : Novel tools for assessment of environmental toxicity"Nakanishi Publishing Co. , Kyoto,

    Japan  1997 

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  • Factors affecting the efficiency of in-vitro production of porcine embryos.

    "Reproductive Biology update : Novel tools for assessment of environmental toxicity"Nakanishi Publishing Co. , Kyoto, Japan  1997 

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  • Current status of in vitro production of porcine embryos.(共著)

    'Advances in Swine Biomedical Research'Plenum Publishing Co., New York, USA  1996 

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  • In vitro maturation and fertilization of pig oocytes.(共著)

    'Beltsville Symposia in Agricultural Research XX. Biotechnology's Role in the Genetic Improvement of Farm Animals' The American Society of Animal Science, Savoy, IL, USA  1996 

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MISC

  • 哺乳動物初期胚への光による時空間特異的なRNA導入法

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    日本光医学・光生物学会   45th   2023

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  • Spatiotemporally photocontrolled RNA transfection in early mammalian embryos

    井川優風, 若井拓哉, 舟橋弘晃, 渡邉和則, 大槻高史

    日本バイオマテリアル学会大会予稿集(Web)   44th   2022

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  • スマート酪農の海外事情 2)各種機関の連携により牛群情報をリアルタイムに酪農場へ提供 最新技術導入とデータ共有進めるイスラエル その2

    舟橋弘晃

    Dairyman   71 ( 8 )   2021

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  • 着床前胚発生におけるミトコンドリア分裂因子Drp1の機能解析

    野村瑠莉, 月向はるな, 舟橋弘晃, 若井拓哉

    Journal of Mammalian Ova Research   36 ( 1 )   S43 - S43   2019.4

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    Language：Japanese   Publisher：(一社)日本卵子学会  

    J-GLOBAL

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  • 体外成熟時のブタ卵母細胞のオートファジー能は由来する小中卵胞で異なる

    小野千由貴, 若井拓哉, 舟橋弘晃

    Journal of Mammalian Ova Research   36 ( 1 )   S56 - S56   2019.4

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    Language：Japanese   Publisher：(一社)日本卵子学会  

    J-GLOBAL

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  • Trim‐Away法を用いたマウス卵母細胞におけるDrp1の機能解析

    月向はるな, 野村瑠莉, 舟橋弘晃, 若井拓哉

    Journal of Mammalian Ova Research   36 ( 1 )   S44 - S44   2019.4

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    Language：Japanese   Publisher：(一社)日本卵子学会  

    J-GLOBAL

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  • 体外成熟時のブタ卵母細胞のオートファジー能は由来する小中卵胞で異なる

    小野 千由貴, 若井 拓哉, 舟橋 弘晃

    Journal of mammalian ova research   36 ( 1 )   S56 - S56   2019.4

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    Language：Japanese   Publisher：(一社)日本卵子学会  

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  • Trim-Away法を用いたマウス卵母細胞におけるDrp1の機能解析

    月向 はるな, 野村 瑠莉, 舟橋 弘晃, 若井 拓哉

    Journal of mammalian ova research   36 ( 1 )   S44 - S44   2019.4

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  • 着床前胚発生におけるミトコンドリア分裂因子Drp1の機能解析

    野村 瑠莉, 月向 はるな, 舟橋 弘晃, 若井 拓哉

    Journal of mammalian ova research   36 ( 1 )   S43 - S43   2019.4

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  • Are changes on the extracellular environment another factor contributing to the loss of fertility in women?

    プジョルピラー フェレー, 大月純子, 舟橋弘晃, 中塚幹也

    Journal of mammalian ova research   36 ( 1 )   S51 - S51   2019

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  • 光応答的に働く卵活性化因子の開発

    大本和正, 若井拓哉, 舟橋弘晃, 渡邉和則, 大槻高史

    日本分子生物学会年会プログラム・要旨集(Web)   42nd   2019

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  • マウス卵母細胞におけるTrim-Away法を用いたリン酸化タンパク質の分解

    月向はるな, 野村瑠莉, 舟橋弘晃, 若井拓哉

    Journal of reproduction and development   65 ( Suppl Japanese Issue )   j119 - j119   2019

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  • ミトコンドリア分裂因子Drp1の阻害による着床前マウス胚の発生停止

    野村瑠莉, 月向はるな, 舟橋弘晃, 若井拓哉

    Journal of reproduction and development   65 ( Suppl Japanese Issue )   j120 - j120   2019

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  • 受精後のDNAメチル化リプログラミングにおける種間差異

    澤田友季乃, 舟橋弘晃, 若井拓哉

    岡山実験動物研究会報   ( 34 )   76 - 77   2018

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  • ブタ顕微授精卵での精子頭部膨化・雄性前核形成不全の解析

    林雄平, 若井拓哉, 舟橋弘晃

    日本卵子学会誌   3 ( 1 )   S45 - S45   2018

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  • 胚培養士が行う不妊予防啓発授業

    高山修, 大月純子, 舟橋弘晃, 舟橋弘晃, 中塚幹也, 中塚幹也

    日本不妊カウンセリング学会誌   17 ( 1 )   66 - 67   2018

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  • 初期胚におけるミトコンドリア分裂因子Drp1の役割

    野村瑠莉, 若井拓哉, 舟橋弘晃

    日本卵子学会誌   3 ( 1 )   S58 - S58   2018

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  • 異なる還元剤による精子前処理がICSIブタ卵での雄性前核形成に及ぼす影響

    林雄平, 若井拓哉, 舟橋弘晃

    日本畜産学会大会講演要旨   122nd   156 - 156   2017

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  • Epidermal Growth Factorはヒト初期卵胞の体外発育を促進する

    高山修, 奥平裕一, 若井拓哉, 中塚幹也, 中塚幹也, 舟橋弘晃, 舟橋弘晃

    日本卵子学会誌   2 ( 1 )   S58 - S58   2017

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  • 生殖補助医療現場で使用されている手技等に関する全国調査

    高山修, 倉田裕子, 小倉初音, 坂東裕香里, 大和礼, 本橋秀之, 中塚幹也, 中塚幹也, 舟橋弘晃, 舟橋弘晃, 舟橋弘晃

    日本卵子学会誌   2 ( 2 )   59 - 64   2017

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  • Improvement of decreased the farrowing rate after artificial insemination with liquid boar semen during hot season by supplementation of semen extender with caffeine

    山口昇一郎, 金子和典, 金子和典, 川島幸子, 川島幸子, 竹村陽, 竹村陽, 増本憲考, 笠正二郎, 笠正二郎, 舟橋弘晃, 吉岡耕治

    日本養豚学会誌   54 ( 4 )   2017

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  • ウシ精巣からの精原幹細胞採取の試み

    永原知樹, 若井拓哉, 舟橋弘晃

    岡山実験動物研究会報   ( 33 )   50 - 50   2017

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  • ブタ卵母細胞中のミトコンドリア細胞内分布の変化

    葛原大貴, 若井拓哉, 舟橋弘晃

    岡山実験動物研究会報   ( 33 )   50 - 51   2017

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  • クワの実は上皮細胞死を抑制することでインドメタシン誘発性胃潰瘍発症を阻止する

    定兼侑里, 藤原崇子, 大西直規, 村田芳行, 舟橋弘晃, 畑生俊光

    日本農芸化学会大会講演要旨集(Web)   2016   2016

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  • 生殖補助医療現場で使用されている手技等に関する全国調査結果

    高山修, 倉田裕子, 小倉初音, 坂東裕香里, 大和礼, 本橋秀之, 中塚幹也, 中塚幹也, 舟橋弘晃, 舟橋弘晃, 舟橋弘晃

    日本卵子学会誌   1 ( 1 )   S46 - S46   2016

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  • 生殖補助医療技術者の養成とスキルアップに高等教育機関が果たすべき役割

    舟橋弘晃, 高山修, 本橋秀之, 中塚幹也

    日本卵子学会誌   1 ( 1 )   S12 - S12   2016

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  • クワの実凍結乾燥粉末摂取による肥満抑制効果の検討

    畑生俊光, 渡邉智, 藤原崇子, 大西直規, 村田芳行, 舟橋弘晃

    日本農芸化学会大会講演要旨集(Web)   2016   2016

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  • IVM中のブタ小中卵胞由来COC,裸化卵母細胞,卵丘細胞内のcAMPおよびcGMP量

    奥平裕一, 若井拓哉, 舟橋弘晃

    Journal of Reproduction and Development   61 ( Suppl Japanese Issue )   J91 - j91   2015.9

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  • Methods for improving in vitro and in vivo boar sperm fertility International journal

    H. Funahashi

    Reproduction in domestic animals = Zuchthygiene   50   40 - 47   2015.7

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  • クワの実凍結乾燥粉末投与によるインドメタシン誘発性胃潰瘍形成抑制効果の検討

    畑生俊光, 定兼侑里, 藤原崇子, 大西直規, 村田芳行, 舟橋弘晃

    日本農芸化学会大会講演要旨集(Web)   2015   2015

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  • ヒト初期卵胞体外培養時の調整培地の効果

    高山修, 本橋秀之, 奥平裕一, FERRE Pillar, ATHURUPANA Rukmali, MI Bui Thi Tra, 中塚幹也, 中塚幹也, 舟橋弘晃, 舟橋弘晃

    日本IVF学会雑誌   18 ( 2 )   79 - 79   2015

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  • ヒト小卵胞および中卵胞由来卵丘細胞-卵母細胞複合体の体外成熟能

    奥平裕一, 高山修, BUI Thi Tra Mi, FERRE Pilar, 本橋秀之, 若井拓哉, 中塚幹也, 中塚幹也, 舟橋弘晃, 舟橋弘晃

    日本IVF学会雑誌   18 ( 2 )   81 - 81   2015

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  • 段階的な精子の融解はイノシシの精子の融解後の質を改善する(Stepwise thawing enhanced post-thaw quality of boar sperms)

    Athurupana Rukmali, 舟橋 弘晃

    日本畜産学会大会講演要旨集   118回   201 - 201   2014.3

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  • ヒト中卵胞および小卵胞由来裸化卵母細胞の体外成熟能

    奥平裕一, 李楊, RUKMALI Athurupana, PILAR Ferre, 高山修, 本橋秀之, 中塚幹也, 中塚幹也, 舟橋弘晃, 舟橋弘晃

    日本生殖医学会雑誌   59 ( 4 )   391 - 391   2014

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  • ヒト初期卵胞の体外培養(IVG)における培養条件の検討

    高山修, 本橋秀之, 奥平裕一, 李楊, PILAR Ferre, RUKMALI Athurupana, 中塚幹也, 中塚幹也, 舟橋弘晃, 舟橋弘晃

    日本生殖医学会雑誌   59 ( 4 )   317 - 317   2014

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  • 完全合成溶液中での未成熟ブタ卵母細胞のガラス化保存加温温度の検討

    高橋大山, 舟橋弘晃

    Journal of mammalian ova research   31 ( 2 )   S43 - S43   2014

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  • 現役の胚培養士におけるリカレント教育士への意識

    本橋秀之, 高山修, 沖津摂, 沖津摂, 舟橋弘晃, 舟橋弘晃, 中塚幹也, 中塚幹也

    Journal of mammalian ova research   31 ( 2 )   S97 - S97   2014

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  • 中卵胞由来卵丘細胞塊分泌因子は小卵胞由来ブタ卵母細胞の体外成熟能を改善する

    清水舞, 舟橋弘晃, 船橋弘晃

    Journal of mammalian ova research   31 ( 2 )   S103 - S103   2014

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  • ヒト卵巣組織ガラス化保存融解後の生存性はFreshと同等か?

    本橋秀之, 高山修, RUKMALI Athurupana, 奥平裕一, PILAR Ferre, 李楊, 中塚幹也, 舟橋弘晃

    日本生殖医学会雑誌   59 ( 4 )   411 - 411   2014

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  • 完全合成溶液による未成熟ブタ卵丘細胞-卵母細胞複合体のガラス化保存

    高橋大山, 舟橋弘晃

    Journal of reproduction and development   59 ( Suppl Japanese Issue )   j165 - j165   2013

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  • 豚の非外科的胚移植と新しい人工授精技術の実用化・産業化のための研究開発

    吉岡耕治, 舟橋弘晃, 三角浩司, 星宏良, 阿部宏之, 中根崇, 坂上信忠, 柴田貴子, 中野貞雄

    農林水産省農林水産技術会議事務局研究成果   ( 481 )   2013

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  • タンパク質合成阻害剤クロラムフェニコールおよびシクロヘキシミド存在下でのブタ精子の受精能獲得および体外受精能

    奥平裕一, 舟橋弘晃

    Journal of reproduction and development   59 ( Suppl Japanese Issue )   j136 - j136   2013

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  • 子宮内免疫反応制御による豚人工授精技術

    山口昇一郎, 鈴木千恵, 野口倫子, 笠正二郎, 森美幸, 磯崎良寛, 舟橋弘晃, 吉岡耕治

    Journal of reproduction and development   58 ( Suppl Japanese Issue )   j77 - j77   2012

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  • ブタ受精卵の体外生産技術

    舟橋弘晃

    Journal of reproduction and development   58 ( Suppl Japanese Issue )   j76 - j76   2012

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  • 中小卵胞由来ブタ卵丘細胞卵母細胞複合体のヒアルロナン産生能

    中小路宗洋, 舟橋弘晃

    Journal of reproduction and development   58 ( Suppl Japanese Issue )   j97 - j97   2012

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  • グリセリンおよびトレハロースがブタ精液の凍結融解後の精子機能の正常性に及ぼす影響

    廣瀬悠人, 舟橋弘晃, 舟橋弘晃

    Journal of reproduction and development   58 ( Suppl Japanese Issue )   j205 - j205   2012

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  • 豚液状精液へのカフェイン添加が人工授精後の繁殖成績に及ぼす影響

    増本憲考, 山口昇一郎, 笠正二郎, 舟橋弘晃, 吉岡耕治

    日本養豚学会大会講演要旨   97th   2012

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  • 精子内RNA量を指標としたブタ精液の評価の可能性

    奥平裕一, 佐々木真也, 舟橋弘晃

    Journal of reproduction and development   57 ( Suppl Japanese Issue )   j118 - j118   2011

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  • REGULATION OF SEVERAL TRANSCRIPTS DURING IN VITRO MATURATION IN PORCINE OOCYTES COLLECTED FROM DIFFERENT SIZE FOLLICLES

    M. J. Izquierdo-Rico, R. Romar, C. Kohata, H. Funahashi

    REPRODUCTION FERTILITY AND DEVELOPMENT   23 ( 1 )   229 - 230   2011

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  • ANALYSIS OF CORTICAL GRANULE EXUDATE OBTAINED BY CHEMICAL OOCYTE ACTIVATION IN PIGS

    R. Romar, M. J. Izquierdo-Rico, H. Funahashi

    REPRODUCTION FERTILITY AND DEVELOPMENT   23 ( 1 )   221 - 221   2011

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  • 津高牧場への発情発見システム「牛歩」の導入と今後の課題

    舟橋弘晃, 舟橋弘晃, 野久保隆, 片山佳司

    岡山大学農学部センター報告   ( 33 )   2011

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  • Brilliant cresyl blueで判別された中小卵胞由来ブタ卵母細胞の体外成熟能とRNA量

    THANH Lam Thi Ngoc, 舟橋弘晃

    Journal of reproduction and development   57 ( Suppl Japanese Issue )   2011

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  • 豚凍結精液人工授精における精液希釈液へのカフェイン添加が融解精子の膜正常性,子宮内白血球数および炎症性サイトカインmRNA発現に及ぼす影響

    山口昇一郎, 鈴木千恵, 野口倫子, 笠正二郎, 森美幸, 磯崎良寛, 舟橋弘晃, 吉岡耕治

    Journal of reproduction and development   57 ( Suppl Japanese Issue )   j138 - j138   2011

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  • 小卵胞由来体外成熟ブタ卵母細胞の体外受精および前核形成能

    小畑千由貴, 舟橋弘晃

    日本畜産学会大会講演要旨   112th   85 - 85   2010

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  • 卵黄がウシ及びブタ多核白血球の貪食性と走化性に及ぼす影響

    李井春, 舟橋弘晃

    日本畜産学会大会講演要旨   112th   86 - 86   2010

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  • マイクロ空圧駆動型人工卵管で負荷される力学的刺激

    李井春, 松浦宏治, 兒玉美恵子, 黒田ユカ, 舟橋弘晃, 成瀬恵治

    化学とマイクロ・ナノシステム研究会講演要旨集   21st   2010

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  • タンパク質合成阻害剤がブタ精子内RNA量および運動性に及ぼす影響

    山下佳佑, 舟橋弘晃

    Journal of reproduction and development   56 ( Suppl Japanese Issue )   j79 - j79   2010

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  • 分化誘導したマウスES細胞及びiPS細胞におけるCy3標識REIC/Dkk-3タンパク質のエンドサートーシスによる取り込み

    片岡健, 阪口政清, LI Kun Peng, DU Gang, 武田千佳, 舟橋弘晃, 村田等, HUH Nam-Ho

    組織培養研究   29 ( 1 )   74 - 74   2010

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  • ラット胚盤胞の非外科的受精卵移植による産子の獲得

    金時宇, 柏崎直巳, 舟橋弘晃

    Journal of reproduction and development   56 ( Suppl Japanese Issue )   j70 - j70   2010

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  • 動的環境における胚培養成績上昇機構に関する考察

    松浦宏治, 黒田ユカ, 舟橋弘晃, 成瀬恵治

    日本生殖医学会雑誌   55 ( 3 )   97 - 97   2010

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  • 繁殖技術に関する最新動向 グローバルな視点での人工授精の技術開発動向

    舟橋弘晃

    養豚の友   ( 494 )   2010

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  • Reduction of immunological sperm loss following artificial insemination of boar semen

    FUNAHASHI Hiroaki, LI Jing-Chun, YAMAGUCHI Shoichiro, MURAKAMI Tetsuya

    46 ( 4 )   211 - 212   2009.12

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  • 授精のベストタイミングとTop Quality Embryoの獲得 IVM成熟卵子の最適受精条件とは何か?

    舟橋 弘晃

    日本IVF学会誌   12   51 - 53   2009.9

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  • 卵黄含有希釈液の注入がラット子宮内多核白血球数に及ぼす影響

    藪崎雅紀, 舟橋弘晃

    Journal of reproduction and development   55 ( Supplement )   j158 - j158   2009

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  • 小卵胞由来および中卵胞由来ブタ卵母細胞に付随する卵丘細胞の生存性およびアポトーシス頻度の比較

    松永利恵, 舟橋弘晃

    日本畜産学会大会講演要旨   110th   89 - 89   2009

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  • カフェイン及びヘパリンが豚白血球の走化性と貪食性に及ぼす影響

    李井春, 舟橋弘晃

    Journal of reproduction and development   55 ( Supplement )   j158 - j158   2009

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  • シリコンエラストマー製チャンバーによるガラス吸着性精子の運動軌跡測定

    松浦宏治, 山下佳佑, 黒田ユカ, 舟橋弘晃, 舟橋弘晃

    Journal of reproduction and development   55 ( Supplement )   j75 - j75   2009

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  • 卵丘細胞の共培養が小卵胞由来ブタ卵母細胞の成熟率に与える影響

    松永利恵, 舟橋弘晃

    Journal of reproduction and development   55 ( Supplement )   j137 - j137   2009

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  • カフェイン添加希釈液への抗酸化剤の添加が豚凍結融解精子の先体反応及びAI後の繁殖成績に及ぼす影響

    山口昇一郎, 舟橋弘晃, 村上徹哉, 徳満茂

    日本養豚学会大会講演要旨   92nd   2009

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  • PDMSの物性を利用した生殖細胞操作デバイスの作製

    松浦宏治, 松浦宏治, 黒田ユカ, 黒田ユカ, 舟橋弘晃, 成瀬恵治

    化学とマイクロ・ナノシステム研究会講演要旨集   20th   2009

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  • PKC活性化剤によるゴナドトロピン・フリー完全合成培地中でのブタ卵母細胞の体外成熟

    中務結貴, 舟橋弘晃, 舟橋弘晃

    Journal of reproduction and development   55 ( Supplement )   j139 - j139   2009

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  • 傾斜培養によるマウス胚の培養成績とそのマイクロ空間制御

    黒田ユカ, 黒田ユカ, 松浦宏治, 松浦宏治, 舟橋弘晃, 成瀬恵治

    化学とマイクロ・ナノシステム研究会講演要旨集   20th   2009

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  • DIBUTYRYL CYCLIC AMP AND EGF-LIKE FACTORS SUPPORT IN VITRO MATURATION OF PORCINE OOCYTES IN A CHEMICALLY DEFINED MEDIUM WITHOUT GONADOTROPINS

    Y. Akaki, K. Yoshioka, H. Funahashi

    REPRODUCTION FERTILITY AND DEVELOPMENT   21 ( 1 )   218 - 219   2009

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  • 牛多核白血球の精子および血清に対する走化性

    李井春, 薮崎雅紀, 舟橋弘晃, 舟橋弘晃

    Journal of reproduction and development   54 ( Supplement )   j137 - j137   2008

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  • ラットおよびマウス透明帯除去初期胚の体外培養とヒアルロン酸による卵割促進効果

    奥山正信, 舟橋弘晃

    Journal of reproduction and development   54 ( Supplement )   j123 - j123   2008

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  • ラット子宮内多核白血球の性周期および交配後の変動

    薮崎雅紀, 李井春, 舟橋弘晃, 舟橋弘晃

    Journal of reproduction and development   54 ( Supplement )   j137 - j137   2008

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  • 体外成熟時のシェアストレスがブタ卵母細胞の初期発生能に及ぼす影響

    小池孝幸, 成瀬恵治, 舟橋弘晃

    日本畜産学会大会講演要旨   109th   2008

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  • 子宮内免疫制御による豚凍結精液人工授精方法の検討

    山口昇一郎, 舟橋弘晃, 村上徹哉

    日本畜産学会大会講演要旨   109th   2008

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  • 揺動培養によるマウス胚盤胞到達率の上昇

    黒田ユカ, 松浦宏治, 片野坂友紀, 毛利聡, 舟橋弘晃, 成瀬恵治

    日本生体医工学会大会プログラム・論文集(CD-ROM)   47th   2008

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  • 揺動培養器を用いたブタ卵母細胞の体外成熟と単為発生後の初期発生

    舟橋弘晃, 小池孝幸, 成瀬恵治

    日本生殖医学会雑誌   53 ( 1/2 )   44 - 44   2008

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  • 老齢種雄豚精子希釈液へのカルニチン添加が精子ミトコンドリア活性に及ぼす影響

    佐野光, 舟橋弘晃

    日本畜産学会大会講演要旨   109th   2008

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  • ゴナドトロピン・フリー完全合成培地中でのブタ卵母細胞の体外成熟

    赤木友香, 舟橋弘晃

    日本畜産学会大会講演要旨   109th   2008

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  • 哺乳動物卵母細胞の体外成熟の現況

    舟橋 弘晃

    Journal of clinical embryologist   9   4 - 8   2007.6

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  • 限定培地へのPyruvate添加がブタ卵母細胞の核・細胞質成熟に及ぼす影響

    小池孝幸, 坂井涼子, 舟橋弘晃

    日本畜産学会大会講演要旨   108th   67 - 67   2007

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  • グルコースおよびピルビン酸がブタ卵母細胞の核成熟およびグルタチオン濃度に及ぼす影響

    小池孝幸, 舟橋弘晃

    日本畜産学会大会講演要旨   107th   74 - 74   2007

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  • カルニチンがブタ卵母細胞の体外成熟に及ぼす影響

    赤木友香, 舟橋弘晃

    日本畜産学会大会講演要旨   108th   67 - 67   2007

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  • 小中卵胞由来ブタ卵母細胞-卵丘細胞塊におけるグルコース6リン酸脱水素酵素活性の所在

    舟橋弘晃, 池原あかり, 石田有希

    日本畜産学会大会講演要旨   107th   74 - 74   2007

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  • Nucleosideが成熟過程のマウス卵母細胞内ミトコンドリア膜電位活性に及ぼす影響

    藤井渉, 舟橋弘晃

    Journal of reproduction and development   53 ( Supplement )   j177 - j177   2007

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  • 豚凍結精液の人工授精で用いる融解液の違いが人工授精後の繁殖成績および豚凍結精子の先体反応に及ぼす影響

    山口昇一郎, 舟橋弘晃, 村上徹哉, 伊東正吾

    日本畜産学会大会講演要旨   106th   101 - 101   2006

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  • 体細胞を導入したラット卵母細胞の活性化後の発生能

    藤井渉, 舟橋弘晃

    Journal of reproduction and development   52 ( Supplement )   j77 - j77   2006

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  • 豚精液の深部注入器によるAI技術の現状 海外および国内の事例から

    舟橋弘晃

    養豚の友   ( 444 )   2006

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  • Select antioxidants improve the function of extended boar semen stored at 10 degrees C International journal

    H Funahashi, T Sano

    THERIOGENOLOGY   63 ( 6 )   1605 - 1616   2005.4

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  • 豚人工授精における技術的課題と技術開発方向 ２．精液注入器の改良．

    舟橋弘晃

    畜産技術   第599号(2005年4月号) pp13-18 ( 599 )   2005

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  • プロテアーゼ阻害剤による体外成熟豚卵子の前処理が体外受精系での精子侵入に及ぼす影響

    舟橋弘晃

    日本畜産学会大会講演要旨   104th   129 - 129   2005

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  • Reduction of the incidence of polyspermic penetration into porcine oocytes by pretreatment of fresh spermatozoa with adenosine and a transient co-incubation of the gametes with caffeine International journal

    H Funahashi, R Romar

    REPRODUCTION   128 ( 6 )   789 - 800   2004.12

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  • Reduction of the incidence of polyspermic penetration into porcine oocytes by pretreatment of fresh spermatozoa with adenosine and a transient co-incubation of the gametes with caffeine International journal

    H Funahashi, R Romar

    REPRODUCTION   128 ( 6 )   789 - 800   2004.12

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  • Development of an effective system to control sperm penetrability for artificial insemination and in vitro fertilization: Utilization of oxidant and anti-oxidant

    舟橋弘晃

    食肉に関する助成研究調査成果報告書   Vol.22, p55-61.   2004

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  • 瞬時共培養法による豚卵子への多精子侵入頻度の軽減

    舟橋弘晃, ROMAR R

    日本畜産学会第103回大会講演要旨   p108   108 - 108   2004

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  • In vitro fertility of boar spermatozoa preserved at 10oC for 22 days.

    Reproduction Fertility and development   16(1,2):255   2004

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  • IVM-IVF of porcine oocytes treated with roscovitine、

    日本畜産学会第103回大会講演要旨   p107   2004

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  • 10℃保存された豚精子の体外受精能と人工授精の成績

    豚の繁殖衛生セミナー通信   30, 7-12   2003

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  • Polyspermic penetration in porcine IVM-IVF systems International journal

    Hiroaki Funahashi

    Reprodution, Fertility and Development   15 ( 3 )   167 - 177   2003

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    Although techniques for in vitro production of porcine embryos have proceeded very rapidly during the past decade, polyspermic penetration still remains a persistent obstacle to porcine in vitro fertilization (IVF) systems. Considerable research on in vitro polyspermic penetration in porcine in vitro-matured (IVM) oocytes has been undertaken to try to solve this problem. In the current paper, recent advancements in overcoming the problems of polyspermy in porcine IVF systems are reviewed. Partial induction of the acrosome reaction of boar spermatozoa in IVF media that contain caffeine is likely to be one of the major causes of polyspermy. A reduction in the number of incompletely acrosome-reacted spermatozoa, which can bind tightly to the zona pellucida and mask free sperm receptors of the zona pellucida, could reduce the incidence of polyspermic penetration; however, morphological differences in the reaction of the zona pellucida have been observed between IVM and ovulated oocytes, which suggests that altered zona morphology may be another cause of polyspermic penetration. It has been shown that the developmental ability of polyspermic porcine embryos to the blastocyst stage is similar to that of normal embryos but that developmental competence to term is much lower. To overcome the current problems of polyspermy, it is suggested that future efforts should be focused on controlling boar sperm function and/or sperm-zona binding to achieve the final maturation associated with normal zona modifications of porcine oocytes at fertilization.

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  • 豚卵母細胞の体外成熟と体外受精

    舟橋弘晃

    日本不妊学会雑誌   48, 274-275 ( 3/4 )   274 - 275   2003

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  • 体外培養システムにおけるブタ卵子の細胞質成熟

    IVF Conference 2003   68 - 71   2003

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  • 哺乳動物初期胚の対外培養および操作が着床後の成長・分化に残す課題

    舟橋 弘晃

    Journal of Chinical Embryologist   6, 15-22   15 - 22   2003

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  • Polyspermic penetration in porcine IVM-IVF systems

    H Funahashi

    REPRODUCTION FERTILITY AND DEVELOPMENT   15 ( 3 )   167 - 177   2003

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    Language：English   Publishing type：Book review, literature introduction, etc.  

    DOI： 10.1071/RD02076

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  • 同種および異種精しょうが豚凍結精子の生存性および受精能に及ぼす効果

    舟橋弘晃, 佐野通

    日本養豚学会大会講演要旨   79th   2003

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  • Induction of capacitation and the acrosome reaction of boar spermatozoa by L-arginine and nitric oxide synthesis associated with the anion transport system International journal

    H Funahashi

    REPRODUCTION   124 ( 6 )   857 - 864   2002.12

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  • Effect of methyl-beta-cyclodextrin and fertilization promoting peptide on capacitation of boar spermatozoa in a protein-free medium

    H Funahashi

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   48 ( 1 )   57 - 63   2002.2

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  • Induction of capacitation and the acroxome reaction of boar spermatozoa by 1-arginine and nitric oxide synthesis associated with the anion transport system.

    Reproduction   124 ( 6 )   857 - 864   2002

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  • カフェインおよび受精促進ペプチド存在下での豚新鮮射出精子のNO産生

    第95回日本繁殖生物学会講演要旨集   p161   2002

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  • 豚凍結精液融解後のグリセリン濃度が精子の受精能に及ぼす影響

    佐野通, 荒金知宏, 舟橋弘晃

    第78回日本養豚学会大会講演要旨集   p11   2002

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  • チオール類が豚液状保存精子の生存性および自発的先体反応に及ぼす影響

    舟橋弘晃, 原田護, 佐野通, 伊藤述史

    第77回日本養豚学会大会講演要旨集   p23   2002

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  • 豚精子のHCO3-/C1-輸送がアルギニンによる受精態獲得・先体反応促進効果に及ぼす影響

    日本畜産学会第100回大会講演要旨集   p115   2002

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  • Effect of methyl-beta-cyclodextrin and fertilization promoting peptide on sperm capacitation of boar spermatozoa in a protein-free medium.

    Hiroaki FUNAHASHI

    Journal of Reproduction and Development   48 ( 1 )   57 - 63   2002

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  • 豚精子のHCO3-/Cl-輸送がアルギニンによる受精能獲得・先体反応促進効果に及ぼす影響

    舟橋弘晃

    日本畜産学会大会講演要旨   100th   2002

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  • Transmission electron microscopy studies of the zona reaction in pig oocytes fertilized in vivo and in vitro International journal

    H Funahashi, H Ekwall, K Kikuchi, H Rodriguez-Martinez

    REPRODUCTION   122 ( 3 )   443 - 452   2001.9

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  • Transmission electron microscopy studies of the zona reaction in pig oocytes fertilized in vivo and in vitro

    H Funahashi, H Ekwall, K Kikuchi, H Rodriguez-Martinez

    REPRODUCTION   122 ( 3 )   443 - 452   2001.9

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  • Regulation of in vitro penetration of frozen-thawed boar spermatozoa by caffeine and adenosine International journal

    H Funahashi, T Nagai

    MOLECULAR REPRODUCTION AND DEVELOPMENT   58 ( 4 )   424 - 431   2001.4

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  • Regulation of in vitro penetration of frozen-thawed boar spermatozoa by caffeine and adenosine

    H Funahashi, T Nagai

    MOLECULAR REPRODUCTION AND DEVELOPMENT   58 ( 4 )   424 - 431   2001.4

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  • アルギニンが豚精子の受精能獲得・先体反応に及ぼす影響

    舟橋弘晃

    日本畜産学会第98回大会講演要旨   98th   110   2001

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  • 緑色蛍光蛋白質の顕微注入法と核移植技術を組み合わせた形質転換牛胚の効率的生産手法の開発

    第12回西日本胚移植研究会・第20回北海道牛受精卵移植研究会合同研究発表大会講演要旨集   36   2001

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  • Effect of fertilization promoting peptide and methyl-beta-cyclodextrin on sperm capacitation of boar spermatozoa in a protein-free medium.

    Sixth International Conference on Pig Reproduction   76   2001

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  • Nuclear transfer of blastomeres expressing EGFP-reporter gene may improve the efficiency of transgenic cattle

    Hiroaki Funahashi, A. Ideta, M. Konishi, M. Urakawa, K. Uruno, Y. Aoyagi, M. Okabe, K. Niwa

    Cloning and Stem Cells   3 ( 4 )   183 - 190   2001

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    DOI： 10.1089/15362300152725891

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  • Oviduct involvement in sperm capacitation and oocyte development

    Sixth International Conference on Pig Reproduction   62   2001

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  • An efficient method to produce transgenic cattle by using microinjection with an EGFP-reporter and nuclear transfer.

    Theriogenology   55 ( 1 )   522   2001

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  • 豚の新しい繁殖技術とその展望

    舟橋弘晃

    豚の繁殖衛生セミナー通信   27 ( 28 )   79 - 88   2001

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  • Effect of fertilization promoting peptide and methyl-beta-cyclodextrin on sperm capacitation of boar spermatozoa in a protein-free medium.

    Sixth International Conference on Pig Reproduction   76   2001

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  • Nuclear transfer of blastomeres expressing EGFP-reporter gene may improve the efficiency of transgenic cattle

    Hiroaki Funahashi, A. Ideta, M. Konishi, M. Urakawa, K. Uruno, Y. Aoyagi, M. Okabe, K. Niwa

    Cloning and Stem Cells   3 ( 4 )   183 - 190   2001

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    DOI： 10.1089/15362300152725891

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  • An efficient method to produce transgenic cattle by using microinjection with an EGFP-reporter and nuclear transfer.

    Theriogenology   55 ( 1 )   522   2001

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  • Involvement of oviduct in sperm capacitation and oocyte development in pigs. International journal

    H. Rodriguez-Martinez, P. Tienthai, K. Suzuki, H. Funahashi, H. Ekwall, A. Johannisson

    Reproduction (cambridge, england) supplement   58   129 - 145   2001

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  • Zona reaction in porcine oocytes fertilized in vivo and in vitro as seen with scanning electron microscopy International journal

    H Funahashi, H Ekwall, H Rodriguez-Martinez

    BIOLOGY OF REPRODUCTION   63 ( 5 )   1437 - 1442   2000.11

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  • Zona reaction in porcine oocytes fertilized in vivo and in vitro as seen with scanning electron microscopy

    H Funahashi, H Ekwall, H Rodriguez-Martinez

    BIOLOGY OF REPRODUCTION   63 ( 5 )   1437 - 1442   2000.11

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  • Modulation of the function of boar spermatozoa via adenosine and fertilization promoting peptide receptors reduce the incidence of polyspermic penetration into porcine oocytes

    H Funahashi, T Fujiwara, T Nagai

    BIOLOGY OF REPRODUCTION   63 ( 4 )   1157 - 1163   2000.10

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  • Modulation of the function of boar spermatozoa via adenosine and fertilization promoting peptide receptors reduce the incidence of polyspermic penetration into porcine oocytes International journal

    H Funahashi, T Fujiwara, T Nagai

    BIOLOGY OF REPRODUCTION   63 ( 4 )   1157 - 1163   2000.10

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  • 越壁IVF法による精子選別は豚卵子への多精子受精頻度を減少させる

    舟橋 弘晃, 永井 卓

    The journal of reproduction and development   46 ( 5 )   319 - 324   2000.10

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  • Both fertilization promoting peptide and adenosine stimulate capacitation but inhibit spontaneous acrosome loss in ejaculated boar spermatozoa in vitro International journal

    H Funahashi, A Asano, T Fujiwara, T Nagai, K Niwa, LR Fraser

    MOLECULAR REPRODUCTION AND DEVELOPMENT   55 ( 1 )   117 - 124   2000.1

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  • Both fertilization promoting peptide and adenosine stimulate capacitation but inhibit spontaneous acrosome loss in ejaculated boar spermatozoa in vitro

    H Funahashi, A Asano, T Fujiwara, T Nagai, K Niwa, LR Fraser

    MOLECULAR REPRODUCTION AND DEVELOPMENT   55 ( 1 )   117 - 124   2000.1

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  • Sperm selection by a climbing-over-a-wall method reduces the incidence of polyspermic penetration of porcine oocytes.

    Hiroaki FUNAHASHI, Takashi NAGAI

    Journal of Reproduction and Development   46 ( 5 )   319 - 324   2000

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  • Sperm Selection by a Climbing-over-a-Wall IVF Method Reduces the Incidence of Polyspermic Penetration of Porcine Oocytes

    Hiroaki Funahashi

    Journal of Reproduction and Development   46 ( 5 )   319 - 324   2000

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    DOI： 10.1262/jrd.46.319

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  • Ultrastructure of the zona reaction of in vivo and in vitro fertilized pig oocytes. (Other,)

    The 14th International Congress on Animal Reproduction   2000

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  • Ultrastructure of the zona reaction of in vivo and in vitro fertilized pig oocytes.

    The 14th International Congress on Animal Reproduction   2000

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  • 豚精子の受精能獲得を調節するアデノシン・レセプターの役割

    舟橋弘晃, 藤原俊満

    日本畜産学会第97回大会講演要旨   97th   124   2000

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  • The zona reaction in porcine oocytes fertilized in vivo and in vitro as seen with scanning electron microscopy.

    Biology of Reproduction   63 ( 5 )   1447 - 1452   2000

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  • The zona reaction in porcine oocytes fertilized in vivo and vitro as seen with scanning electron microscopy.

    Theriogenology   53 ( 1 )   420   2000

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  • Changes in intracellular content of glutathione and thiols associated with gamma-glutamyl cycle during sperm penetration and pronuclear formation in rat oocytes

    H Funahashi, N Bandoh, S Nakahira, SH Oh, S Tsuboi

    ZYGOTE   7 ( 4 )   301 - 305   1999.11

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  • Changes in intracellular content of glutathione and thiols associated with gamma-glutamyl cycle during sperm penetration and pronuclear formation in rat oocytes International journal

    H Funahashi, N Bandoh, S Nakahira, SH Oh, S Tsuboi

    ZYGOTE   7 ( 4 )   301 - 305   1999.11

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  • Recent development in embryo technology in pigs - Review

    K Niwa, H Funahashi

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES   12 ( 6 )   966 - 975   1999.9

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  • Recent development in embryo technology in pigs - Review

    K Niwa, H Funahashi

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES   12 ( 6 )   966 - 975   1999.9

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    Language：English   Publishing type：Book review, literature introduction, etc.  

    DOI： 10.5713/ajas.1999.966

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  • DNA stability and thiol-disulphide status of rat sperm nuclei during epididymal maturation and penetration of oocytes

    S Said, H Funahashi, K Niwa

    ZYGOTE   7 ( 3 )   249 - 254   1999.8

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  • DNA stability and thiol-disulphide status of rat sperm nuclei during epididymal maturation and penetration of oocytes International journal

    S Said, H Funahashi, K Niwa

    ZYGOTE   7 ( 3 )   249 - 254   1999.8

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  • Production of plasminogen activators (PAs) in bovine cumulus-oocyte complexes during maturation in vitro: Effects of epidermal growth factor on production of PAs in oocytes and cumulus cells

    KW Park, SH Choi, XX Song, H Funahashi, K Niwa

    BIOLOGY OF REPRODUCTION   61 ( 1 )   298 - 304   1999.7

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  • Production of plasminogen activators (PAs) in bovine cumulus-oocyte complexes during maturation in vitro: Effects of epidermal growth factor on production of PAs in oocytes and cumulus cells International journal

    KW Park, SH Choi, XX Song, H Funahashi, K Niwa

    BIOLOGY OF REPRODUCTION   61 ( 1 )   298 - 304   1999.7

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  • 卵胞膜細胞との共培養による卵丘付着牛卵母細胞の卵核胞発育阻害を伴わない減数分裂の休止

    朴 光旭, 舟橋 弘晃, 丹羽 晧二

    The journal of reproduction and development   45 ( 3 )   223 - 231   1999.6

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  • Co-culture of cumulus-enclosed bovine oocytes with theca cells induces the meiotic arrest but does not inhibit germinal vesicle development

    Kwang Wook Park, Hiroaki Funahashi, Koji Niwa

    Journal of Reproduction and Development   45 ( 3 )   223 - 231   1999

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    DOI： 10.1262/jrd.45.223

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  • The presence of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) during meiosis improves porcine 'oocyte competence' as determined by early embryonic development after in-vitro fertilization

    Hiroaki Funahashi, Eric W. McIntush, Michael F. Smith, Billy N. Day

    Journal of Reproduction and Development   45 ( 4 )   265 - 271   1999

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  • 卵子への侵入過程におけるラット精子核内チオール類の酸化還元状態の観察と変化(共著)

    SAID S, 舟橋弘晃, 丹羽こう二

    第95回日本畜産学会大会講演要旨   95th   79 - 79   1999

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  • Co-culture of cumulus-enclosed bovine oocytes with theca cells induces the meiotic arrest but does not inhibit germinal vesicle development

    Kwang Wook Park, Hiroaki Funahashi, Koji Niwa

    Journal of Reproduction and Development   45 ( 3 )   223 - 231   1999

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    DOI： 10.1262/jrd.45.223

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  • The presence of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) during meiosis improves porcine 'oocyte competence' as determined by early embryonic development after in-vitro fertilization

    Hiroaki Funahashi, Eric W. McIntush, Michael F. Smith, Billy N. Day

    Journal of Reproduction and Development   45 ( 4 )   265 - 271   1999

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    DOI： 10.1262/jrd.45.265

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  • Rat oocytes fertilized in modified rat 1-cell embryo culture medium containing a high sodium chloride concentration and bovine serum albumin maintain developmental ability to the blastocyst stage International journal

    SH Oh, K Miyoshi, H Funahashi

    BIOLOGY OF REPRODUCTION   59 ( 4 )   884 - 889   1998.10

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  • Rat oocytes fertilized in modified rat 1-cell embryo culture medium containing a high sodium chloride concentration and bovine serum albumin maintain developmental ability to the blastocyst stage

    SH Oh, K Miyoshi, H Funahashi

    BIOLOGY OF REPRODUCTION   59 ( 4 )   884 - 889   1998.10

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  • Both bovine oocytes and cumulus cells produce plasminogen activator during in vitro maturation

    SH Choi, H Funahashi, K Niwa

    THERIOGENOLOGY   49 ( 1 )   309 - 309   1998.1

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  • Both bovine oocytes and cumulus cells produce plasminogen activator during in vitro maturation

    SH Choi, H Funahashi, K Niwa

    THERIOGENOLOGY   49 ( 1 )   309 - 309   1998.1

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  • EPP and adenosine inhibit spontaneous acrosome loss in boar sperm.

    Journal of Reproduction and Fertility, Abstract Series   21   10   1998

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  • Co-culture of bovine cumulus-oocyte complexes with theca cells inhibits the meiotic resumption at a specific phase of germinal vesicle.

    KW Park, H Funahashi, K Niwa

    BIOLOGY OF REPRODUCTION   58 ( Suppl.1 )   219 - 219   1998

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  • Viability of boar spermatozoa frozen in 0.5ml-plastic straw by 2-step freezing method(共著)

    Proceedings of the 8th World Conference on Animal Production, Contributed Papers   2   252 - 253   1998

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  • Recent Development in Embryo Technology in Pigs. (共著)

    Proceedings of the 8th World Conference on Animal Production, Symposium Series   2   240 - 251   1998

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  • Cytoplasmic maturation of porcine oocytes for successful male pronuclear formation and early embryonic development

    87   205 - 214   1998

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  • EPP and adenosine inhibit spontaneous acrosome loss in boar sperm.

    Journal of Reproduction and Fertility, Abstract Series   21   10   1998

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  • Intracellular calcium release and cortical reaction after thimerosal-treatment of porcine oocytes matured in vitro or ovulated.

    H Funahashi, Z Machaty, WH Wang, TC Cantley, RS Prather, BN Day

    BIOLOGY OF REPRODUCTION   58 ( Suppl.1 )   219 - 219   1998

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  • Inhibiting effect of theca cells on meiotic resumption of bovine oocytes may not be via cumulus cells(共著)

    Proceedings of the 8th World Conference on Animal Production, Contributed PApers   2   920 - 921   1998

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  • Effects of cumulus cells on the ability of pig oocytes to utilize cysteine or cystine during maturation in vitro

    Ken Sawai, Hiroaki Funahashi, Koji Niwa

    Journal of Reproduction and Development   44 ( 2 )   161 - 168   1998

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    DOI： 10.1262/jrd.44.161

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  • Effects of cumulus cells on the ability of pig oocytes to utilize cysteine or cystine during maturation in vitro

    Ken Sawai, Hiroaki Funahashi, Koji Niwa

    Journal of Reproduction and Development   44 ( 2 )   161 - 168   1998

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    DOI： 10.1262/jrd.44.161

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  • Recent Development in Embryo Technology in Pigs. (共著)

    Proceedings of the 8th World Conference on Animal Production, Symposium Series   2   240 - 251   1998

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  • Cytoplasmic maturation of porcine oocytes for successful male pronuclear formation and early embryonic developmentCytoplasmic maturation of porcine oocytes for successful male pronuclear formation and early embryonic development

    87   205 - 214   1998

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  • Viability of boar spermatozoa frozen in 0.5ml-plastic straw by 2-step freezing method(共著)

    Proceedings of the 8th World Conference on Animal Production, Contributed Papers   2   252 - 253   1998

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  • In vitro production of porcine embryos: On the developmental competence.

    舟橋弘晃

    Journal of reproduction and development   44 ( 6 )   j47-j52 - j52   1998

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  • 受精促進ペプチド(FPP)およびアデノシンの豚射出精液に及ぼす自発的先体反応阻止作用における競合(共著)

    浅野敦之, 舟橋弘晃, 丹羽こう二, FRASER L R

    第91回日本繁殖生物学会講演要旨集   91st ( 6 )   45 - a24   1998

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  • リン酸によるラット初期胚の発生阻害時期とマイクロフィラメント構造への影響(共著)

    呉世薫, 舟橋弘晃, 丹羽こう二

    第91回日本繁殖生物学会講演要旨集   91st ( 6 )   43 - a22   1998

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  • Co-culture of bovine cumulus-oocyte complexes with theca cells inhibits the meiotic resumption at a specific phase of germinal vesicle.

    KW Park, H Funahashi, K Niwa

    BIOLOGY OF REPRODUCTION   58 ( Suppl.1 )   219 - 219   1998

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  • Intracellular calcium release and cortical reaction after thimerosal-treatment of porcine oocytes matured in vitro or ovulated.

    H Funahashi, Z Machaty, WH Wang, TC Cantley, RS Prather, BN Day

    BIOLOGY OF REPRODUCTION   58 ( Suppl.1 )   219 - 219   1998

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  • Inhibiting effect of theca cells on meiotic resumption of bovine oocytes may not be via cumulus cells(共著)

    Proceedings of the 8th World Conference on Animal Production, Contributed PApers   2   920 - 921   1998

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  • 受精促進ペプチド(FPP)およびアデノシンが豚精子の自発的先体反応に及ぼす阻止効果(共著)

    15 ( 2 )   S26   1998

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  • Extracorporeal production and artificial operation of fertilized egg of pig.

    舟橋弘晃

    家畜人工授精   ( 185 )   14 - 22   1998

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  • 豚受精卵の体外生産技術の現状

    日本畜産学会関西支部報   ( 138 )   18 - 20   1998

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  • Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization

    H Funahashi, TC Cantley, BN Day

    BIOLOGY OF REPRODUCTION   57 ( 1 )   49 - 53   1997.7

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  • Stage-specific requirement of cysteine during in vitro maturation of porcine oocytes for glutathione synthesis associated with male pronuclear formation International journal

    Ken Sawai, Hiroaki Funahashi, Koji Niwa

    Biology of Reproduction   57 ( 1 )   1 - 6   1997.7

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  • Stage-specific requirement of cysteine during in vitro maturation of porcine oocytes for glutathione synthesis associated with male pronuclear formation

    K Sawai, H Funahashi, K Niwa

    BIOLOGY OF REPRODUCTION   57 ( 1 )   1 - 6   1997.7

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  • Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization International journal

    H Funahashi, TC Cantley, BN Day

    BIOLOGY OF REPRODUCTION   57 ( 1 )   49 - 53   1997.7

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  • Chlortetracycline fluorescence patterns and in vitro fertilisation of frozen-thawed boar spermatozoa incubated under various bicarbonate concentrations International journal

    LR Abeydeera, H Funahashi, NH Kim, BN Day

    ZYGOTE   5 ( 2 )   117 - 125   1997.5

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  • Chlortetracycline fluorescence patterns and in vitro fertilisation of frozen-thawed boar spermatozoa incubated under various bicarbonate concentrations

    LR Abeydeera, H Funahashi, NH Kim, BN Day

    ZYGOTE   5 ( 2 )   117 - 125   1997.5

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  • Developmental changes in the intracellular Ca2+ release mechanisms in porcine oocytes

    Z Machaty, H Funahashi, BN Day, RS Prather

    BIOLOGY OF REPRODUCTION   56 ( 4 )   921 - 930   1997.4

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  • Developmental changes in the intracellular Ca2+ release mechanisms in porcine oocytes International journal

    Z Machaty, H Funahashi, BN Day, RS Prather

    BIOLOGY OF REPRODUCTION   56 ( 4 )   921 - 930   1997.4

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  • Preincubation of cumulus-oocyte complexes before exposure to gonadotropins improves the developmental competence of porcine embryos matured and fertilized in vitro. International journal

    H Funahashi, T C Cantley, B N Day

    Theriogenology   47 ( 3 )   679 - 86   1997.2

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    The objectives of the present study were to examine whether delayed exposure of porcine cumulus-oocyte complexes (COCs) to gonadotropins affects the diameter of oocytes, the nuclear morphology of the germinal vesicle, the rate of germinal vesicle breakdown (GVBD), and the embryonic developmental rate of inseminated oocytes following maturation and fertilization in vitro (IVM/IVF). After preincubation (experimental) or no preincubation (control) in BSA-free NCSU23 medium containing 1096 porcine follicular fluid for 12 h, COCs were cultured for maturation in the same medium supplemented with gonadotropins for 20 h and then without those gonadotropins for 20 h. During the preincubation period, the nuclear morphology of the germinal vesicles became more homogeneous. Incidence of GVBD after 20 h of maturation culture was not different between the control and experimental group. When cultured in NCSU23 medium for 7 d following IVF, the incidence of embryos that developed to the blastocyst stage (23.1 +/- 3.1%) was higher in the experimental group than in the control group (8.7 +/- 1.2%). Blastocysts in the experimental group had a larger number of cells than control blastocysts. Following embryo transfer into the oviduct of recipient gilts, IVM/IVF embryos had elongated by Day 12 of gestation. These results indicate that preincubation of porcine COCs, before exposure to gonadotropins to induce the resumption of meiosis, increases the rate of development of IVM/IVF embryos to the blastocyst stage.

    DOI： 10.1016/S0093-691X(97)00026-5

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  • Additive effects of sodium hyaluronate as inducer of capacitation of boar spermatozoa in vitro.

    Fifth International Conference on Pig Reproduction   142   1997

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  • Production of hyaluronic acid by porcine oocyte-cumulus cell complexes during in vitro maturation.

    Fifth International Conference on Pig Reproduction   139   1997

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  • Tris-buffered medium (TBM) is better for in vitro fertilization of porcine oocytes than mM199 which has been traditionally used.

    H Funahashi, Y Kajiwara, K Sawai, K Niwa

    BIOLOGY OF REPRODUCTION   56 ( Supplementl )   514 - 514   1997

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  • Effect of tissue inhibitor of metalloproteinase(TIMP-1)on early development of seine oocytes matured and fertilized in vitro(共著)

    Theriogenology   46 ( 1 )   277   1997

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  • Effects of pig transparent layer sugar moiety on localization and spermatozoon combination/invasion.

    宋学雄, 楊信志, 舟橋弘晃, 丹羽こう二

    日本繁殖生物学会講演要旨   ( 90th )   56   1997

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  • クローン動物とコピー動物

    岡山地区高分子懇話会平成9年度第2回高分子講演会   11 - 18   1997

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  • Advances in in vitro production of pig embryos

    H Funahashi, BN Day

    JOURNAL OF REPRODUCTION AND FERTILITY   Supplement52   271 - 283   1997

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  • Additive effects of sodium hyaluronate as inducer of capacitation of boar spermatozoa in vitro.

    Fifth International Conference on Pig Reproduction   142   1997

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  • Production of hyaluronic acid by porcine oocyte-cumulus cell complexes during in vitro maturation.

    Fifth International Conference on Pig Reproduction   139   1997

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  • Effect of tissue inhibitor of metalloproteinase (TIMP-1) on early development of swine oocytes matured and fertilized in vitro

    FUNAHASHI H

    Theriogenology   47 ( 1 )   277 - 277   1997

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  • Tris-buffered medium (TBM) is better for in vitro fertilization of porcine oocytes than mM199 which has been traditionally used.

    H Funahashi, Y Kajiwara, K Sawai, K Niwa

    BIOLOGY OF REPRODUCTION   56 ( Supplementl )   514 - 514   1997

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  • Advances in in vitro production of pig embryos

    H Funahashi, BN Day

    JOURNAL OF REPRODUCTION AND FERTILITY   Supplement52   271 - 283   1997

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  • Effects of cysteine in serum-free maturation medium on male pronuclear formation of maturing pig oocytes penetrated in vitro

    Ken Sawai, Hiroaki Funahashi, Wei Hua Wang, Koji Niwa

    Journal of Reproduction and Development   43 ( 1 )   73 - 80   1997

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    DOI： 10.1262/jrd.43.73

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  • Effects of cysteine in serum-free maturation medium on male pronuclear formation of maturing pig oocytes penetrated in vitro

    Ken Sawai, Hiroaki Funahashi, Wei Hua Wang, Koji Niwa

    Journal of Reproduction and Development   43 ( 1 )   73 - 80   1997

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    DOI： 10.1262/jrd.43.73

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  • Preincubation of cumulus-oocyte complexes before exposure to gonadotropins improves the developmental competence of porcine embryos matured and fertilized in vitro

    H. Funahashi, T. C. Cantley, B. N. Day

    Theriogenology   47 ( 3 )   679 - 686   1997

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  • Freezing preservation of pig spermatozoon using a 0.5ml straw for cattle semen.

    野上与志郎, 原田護, 関哲生, 舟橋弘晃, 丹羽こう二

    日本畜産学会大会講演要旨   93rd   1997

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  • Effect of NaCl concentration in fertilization medium on sperm penetration and ealry development of rat eggs.

    OH S-H, 舟橋弘晃, 丹羽こう二

    Journal of mammalian ova research   14 ( 1 )   1997

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  • Advances in in vitro production of pig embryos. International journal

    H. Funahashi, B. N. Day

    Journal of reproduction and fertility. Supplement   52   271 - 283   1997

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  • gamma-Glutamyl transpeptidase of spermatozoa may decrease oocyte glutathione content at fertilization in pigs. International journal

    H Funahashi, Z Machaty, R S Prather, B N Day

    Molecular reproduction and development   45 ( 4 )   485 - 90   1996.12

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    The presence of gamma-glutamyl transpeptidase (GGT) in boar spermatozoa and the potential role of the GGT at sperm penetration were examined using in vitro matured porcine oocytes. In the first experiment, GGT of boar spermatozoa was examined using a histochemical stain. GGT was detected in the midpiece and the acrosome regions of boar spermatozoa. In the second experiment, porcine oocytes matured in vitro were injected with approximately 40 pl of 10 mM HEPES solution alone or HEPES containing 0.5 U/ml GGT or 1 mM guanosine-5'-O-(3'-thiotriphosphate) (GTP-gamma-S; G-protein activator). When GGT was injected into oocytes, the incidence of oocytes activated (23.7 +/- 1.4%) was not different (P > 0.05) from HEPES-injected controls (24.9 +/- 1.3%) at 6 h after injection. Injected GTP-gamma-S, however, activated 76.0 +/- 5.3% of oocytes at 6 h after injection, but extrusion of the second polar body was very low (2.8 +/- 4.8%). Total content of glutathione (GSH) and glutathione disulfide (GSSG) did not differ (P > 0.05) between GTP-gamma-S injected oocytes (4.2 +/- 0.7 pmol/oocyte) and noninjected oocytes (4.0 +/- 0.1 pmol/oocyte) at 6 h after injection. However, the total content of GSH and GSSG was lower (P < 0.01) in GGT-injected oocytes (2.1 +/- 0.2 pmol/oocyte) than HEPES-injected oocytes (3.4 +/- 0.2 pmol/oocyte) at 6 h after injection. In the third experiment, in vitro matured porcine oocytes were injected with about 40 pl of 10 mM HEPES solution alone or HEPES containing 0.5 U/ml GGT and then inseminated. At 12 h after insemination, the incidence of male pronuclear formation was significantly lower in oocytes injected with GGT as compared with injected control oocytes. These results demonstrated that (1) GGT was present on the surface of spermatozoa, (2) total oocyte content of GSH and GSSG was decreased by microinjection of GGT but not by that of GTP-gamma-S, and (3) male pronuclear formation was inhibited in GGT-injected oocytes. These results suggest that sperm GGT may be a limiting factor for male pronuclear formation in polyspermic oocytes.

    DOI： 10.1002/(SICI)1098-2795(199612)45:4<485::AID-MRD11>3.0.CO;2-W

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  • Microtubule organization in porcine oocytes during fertilization and parthenogenesis International journal

    NH Kim, C Simerly, H Funahashi, G Schatten, BN Day

    BIOLOGY OF REPRODUCTION   54 ( 6 )   1397 - 1404   1996.6

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  • Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes

    H Funahashi, NH Kim, TT Stumpf, TC Cantley, BN Day

    BIOLOGY OF REPRODUCTION   54 ( 6 )   1412 - 1419   1996.6

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  • Microtubule organization in porcine oocytes during fertilization and parthenogenesis

    NH Kim, C Simerly, H Funahashi, G Schatten, BN Day

    BIOLOGY OF REPRODUCTION   54 ( 6 )   1397 - 1404   1996.6

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  • Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes International journal

    H Funahashi, NH Kim, TT Stumpf, TC Cantley, BN Day

    BIOLOGY OF REPRODUCTION   54 ( 6 )   1412 - 1419   1996.6

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  • Effects of oviductal fluid on sperm penetration and cortical granule exocytosis during fertilization of pig oocytes in vitro International journal

    NH Kim, H Funahashi, LR Abeydeera, SJ Moon, RS Prather, BN Day

    JOURNAL OF REPRODUCTION AND FERTILITY   107 ( 1 )   79 - 86   1996.5

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  • Effects of injecting calcium chloride into in vitro-matured porcine oocytes International journal

    Z Machaty, H Funahashi, MA Mayes, BN Day, RS Prather

    BIOLOGY OF REPRODUCTION   54 ( 2 )   316 - 322   1996.2

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  • Effects of injecting calcium chloride into in vitro-matured porcine oocytes

    Z Machaty, H Funahashi, MA Mayes, BN Day, RS Prather

    BIOLOGY OF REPRODUCTION   54 ( 2 )   316 - 322   1996.2

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  • Co-culture with follicular shell pieces (FSP) increases the developmental competence of pig oocytes matured in vitro.

    LR Abeydeera, H Funahashi, TC Cantley, A Rieke, BN Day

    BIOLOGY OF REPRODUCTION   54 ( Supplement 1 )   100 - 100   1996

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  • Exposure of swine oocyte-cumulus complexes to dibutyryl cyclic AMP for the first 20 hours of maturation increases the homogeneity of oocyte unclear maturation.(共著)

    Congress Programme of the 13th International Congress on Animal Reproduction   2   10 - 4   1996

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  • In vitro penetration of pig oocytes commercially prepared frozen-thawed boar spermatozoa in a chemically semi-defined bicarbonatefree medium.(共著)

    Theriogenology   45 ( 1 )   265   1996

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  • Gamma-glutamyltranspeptidase of spermatozoa may reduce oocyte glutathione content at sperm penetration(共著)

    Hiroaki Funahashi, Zoltan Machaty, Randall S. Prather, Billy N. Day

    Molecular Reproduction and Development   45 ( 4 )   485 - 490   1996

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  • Factors Affecting Development in Vitro of Bovine and Rat 1-Cell Embryos

    Kazuchika Miyoshi, Hiroaki Funahashi, Koji Niwa

    Journal of Mammalian Ova Research   13 ( 2 )   71 - 80   1996

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  • Factors Affecting Development in Vitro of Bovine and Rat 1-Cell Embryos

    Kazuchika Miyoshi, Hiroaki Funahashi, Koji Niwa

    Journal of Mammalian Ova Research   13 ( 2 )   71 - 80   1996

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  • Factors affecting nuclear and cytoplasmic maturation in pig oocytes

    P Coy, S Ruiz, N Ouhibi, H Funahashi, BN Day, RM Moor

    BIOLOGY OF REPRODUCTION   54 ( Supplement 1 )   412 - 412   1996

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  • Nuclear morphology of swine oocytes during follicular development following stimulation by eCG injection.

    H Funahashi, H Tatemoto, TC Cantley, BN Day

    BIOLOGY OF REPRODUCTION   54 ( Supplement 1 )   408 - 408   1996

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  • Low salt maturation medium enhances the histone H1 kinase activity of porcine oocytes at the end of in vitro maturation.(共著)

    Hiroaki FUNAHASHI, Todd T. STUMPF, Nam-Hyung KIM, Billy N. DAY

    Journal of Reproduction and Development   42 ( 2 )   109 - 115   1996

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  • Effect of saccharides on the semination into a pig ovum.

    宋学雄, 沢井健, 許東けい, 舟橋弘晃, 丹羽こう二

    日本繁殖生物学会講演要旨   89th   57   1996

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  • Effect of cysteine and cystine on the male pronucleus forming ability of pig’s follicular ovum.

    沢井健, 舟橋弘晃, 丹羽こう二

    日本繁殖生物学会講演要旨   89th   44   1996

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  • Effect of somatotropin on the progress rate of the maturation division of cattle’s follicular ovum.

    伊賀浩輔, 舟橋弘晃, 丹羽こう二

    日本繁殖生物学会講演要旨   89th   41   1996

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  • Nuclear morphology of swine oocytes during follicular development following stimulation by eCG injection.

    H Funahashi, H Tatemoto, TC Cantley, BN Day

    BIOLOGY OF REPRODUCTION   54 ( Supplement 1 )   408 - 408   1996

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  • Exposure of swine oocyte-cumulus complexes to dibutyryl cyclic AMP for the first 20 hours of maturation increases the homogeneity of oocyte unclear maturation.(共著)

    Congress Programme of the 13th International Congress on Animal Reproduction   2   10 - 4   1996

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  • In vitro penetration of pig oocytes commercially prepared frozen-thawed boar spermatozoa in a chemically semi-defined bicarbonatefree medium.(共著)

    Theriogenology   45 ( 1 )   265   1996

    　More details

  • Low salt maturation medium enhances the histone H1 kinase activity of porcine oocytes at the end of in vitro maturation.(共著)

    Journal of Reproduction and Development   42 ( 2 )   109 - 115   1996

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  • Factors affecting nuclear and cytoplasmic maturation in pig oocytes

    P Coy, S Ruiz, N Ouhibi, H Funahashi, BN Day, RM Moor

    BIOLOGY OF REPRODUCTION   54 ( Supplement 1 )   412 - 412   1996

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  • Co-culture with follicular shell pieces (FSP) increases the developmental competence of pig oocytes matured in vitro.

    LR Abeydeera, H Funahashi, TC Cantley, A Rieke, BN Day

    BIOLOGY OF REPRODUCTION   54 ( Supplement 1 )   100 - 100   1996

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  • PRONUCLEAR FORMATION AND INTRACELLULAR GLUTATHIONE CONTENT OF IN VITRO-MATURED PORCINE OOCYTES FOLLOWING IN-VITRO FERTILIZATION AND/OR ELECTRICAL ACTIVATION

    H FUNAHASHI, TT STUMPF, TC CANTLEY, NH KIM, BN DAY

    ZYGOTE   3 ( 3 )   273 - 281   1995.8

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  • PRONUCLEAR VISIBILITY, DEVELOPMENT AND TRANSGENE EXPRESSION IN IVM/IVF-DERIVED PORCINE EMBRYOS

    HM KUBISCH, MA LARSON, H FUNAHASHI, BN DAY, RM ROBERTS

    THERIOGENOLOGY   44 ( 3 )   391 - 401   1995.8

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  • PRONUCLEAR FORMATION AND INTRACELLULAR GLUTATHIONE CONTENT OF IN VITRO-MATURED PORCINE OOCYTES FOLLOWING IN-VITRO FERTILIZATION AND/OR ELECTRICAL ACTIVATION International journal

    H FUNAHASHI, TT STUMPF, TC CANTLEY, NH KIM, BN DAY

    ZYGOTE   3 ( 3 )   273 - 281   1995.8

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  • PRONUCLEAR VISIBILITY, DEVELOPMENT AND TRANSGENE EXPRESSION IN IVM/IVF-DERIVED PORCINE EMBRYOS

    HM KUBISCH, MA LARSON, H FUNAHASHI, BN DAY, RM ROBERTS

    THERIOGENOLOGY   44 ( 3 )   391 - 401   1995.8

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  • EFFECT OF TAXOL ON MICROTUBULE ORGANIZATION IN PORCINE OOCYTES AFTER FERTILIZATION AND PARTHENOGENETIC ACTIVATION

    NH KIM, LR ABEYDEERA, RE EVERTS, H FUNAHASHI, BN DAY

    BIOLOGY OF REPRODUCTION   52 ( Supplement 1 )   139 - 139   1995

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  • EFFECT OF BICARBONATE ON IN-VITRO PENETRATION OF PIG OOCYTES BY COMMERCIALLY PREPARED FROZEN-EJACULATED BOAR SEMEN

    LR ABEYDEERA, H FUNAHASHI, NH KIM, BN DAY

    BIOLOGY OF REPRODUCTION   52 ( Supplement 1 )   122 - 122   1995

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  • Effects of organic osmolytes in maturation medium on intracellular glutathione content of porcine oocytes.(共著)

    Theriogenology   43 ( 1 )   213   1995

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  • GAMMA-GLUTAMYL-TRANSPEPTIDASE (GGT) OF SPERMATOZOA MAY REDUCE OOCYTE GLUTATHIONE (GSH) CONTENT AT SPERM PENETRATION

    H FUNAHASHI, Z MACHATY, RS PRATHER, BN DAY

    BIOLOGY OF REPRODUCTION   52 ( Supplement1 )   140 - 140   1995

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  • Effect of exposure of porcine oocyte-cumuluscomplexes to gonadotropins.n.(共著) Beltsville Symposium (]G0010[)(]G0010[) : Biotechnology's

    Role in the Genetic Improvement of Farm Animals   19   1995

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  • Microtubule and microfilament dynamics in porcine oocytes during meiotic maturation International journal

    Nam Hyung Kim, Hiroaki Funahashi, Randall S. Prather, Gerald Schatten, Billy N. Day

    Molecular reproduction and development   43 ( 2 )   248 - 255   1995

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  • Microtuble and microfilament dynamics in porcine oocytes during meiotic maturation.(共著)

    Molecular Reproduction and Development   43 ( 2 )   248 - 255   1995

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  • EFFECT OF TAXOL ON MICROTUBULE ORGANIZATION IN PORCINE OOCYTES AFTER FERTILIZATION AND PARTHENOGENETIC ACTIVATION

    NH KIM, LR ABEYDEERA, RE EVERTS, H FUNAHASHI, BN DAY

    BIOLOGY OF REPRODUCTION   52 ( Supplement 1 )   139 - 139   1995

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  • EFFECT OF BICARBONATE ON IN-VITRO PENETRATION OF PIG OOCYTES BY COMMERCIALLY PREPARED FROZEN-EJACULATED BOAR SEMEN

    LR ABEYDEERA, H FUNAHASHI, NH KIM, BN DAY

    BIOLOGY OF REPRODUCTION   52 ( Supplement 1 )   122 - 122   1995

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  • Effects of cumulus cells on glutathione content of porcine oocyte during in vitro maturation

    FUNAHASHI H

    J Anim Sci   73 ( 1 )   90 - 90   1995

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  • Cytoskeletal reorganization in porcine oocytes after chemical activation.(共著)

    Journal of Animal Science   73 ( Supplement 1 )   90   1995

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  • Comparative expression patterns of two transgenes in murine,porcine and bovine embryos.(共著)

    Theriogenology   43 ( 1 )   262   1995

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  • Effects of organic osmolytes in maturation medium on intracellular glutathione content of porcine oocytes.(共著)

    Theriogenology   43 ( 1 )   213   1995

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  • GAMMA-GLUTAMYL-TRANSPEPTIDASE (GGT) OF SPERMATOZOA MAY REDUCE OOCYTE GLUTATHIONE (GSH) CONTENT AT SPERM PENETRATION

    H FUNAHASHI, Z MACHATY, RS PRATHER, BN DAY

    BIOLOGY OF REPRODUCTION   52 ( Supplement1 )   140 - 140   1995

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  • Effect of exposure of porcine oocyte-cumuluscomplexes to gonadotropins.n.(共著)

    Beltsville Symposium (]G0010[)(]G0010[) : Biotechnology's Role in the Genetic Improvement of Farm Animals   19   1995

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  • Effects of cumulus cells on glutathione content of porcine oocytes during in vitro maturation.(共著)

    Journal of Animal Science   73 ( Supplement 1 )   90   1995

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  • Cytoskeletal reorganization in porcine oocytes after chemical activation.(共著)

    Journal of Animal Science   73 ( Supplement 1 )   90   1995

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  • Comparative expression patterns of two transgenes in murine,porcine and bovine embryos.(共著)

    Theriogenology   43 ( 1 )   262   1995

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  • USE OF LOW-SALT CULTURE-MEDIUM FOR IN-VITRO MATURATION OF PORCINE OOCYTES IS ASSOCIATED WITH ELEVATED OOCYTE GLUTATHIONE LEVELS AND ENHANCED MALE PRONUCLEAR FORMATION AFTER IN-VITRO FERTILIZATION

    H FUNAHASHI, TC CANTLEY, TT STUMPF, SL TERLOUW, BN DAY

    BIOLOGY OF REPRODUCTION   51 ( 4 )   633 - 639   1994.10

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  • USE OF LOW-SALT CULTURE-MEDIUM FOR IN-VITRO MATURATION OF PORCINE OOCYTES IS ASSOCIATED WITH ELEVATED OOCYTE GLUTATHIONE LEVELS AND ENHANCED MALE PRONUCLEAR FORMATION AFTER IN-VITRO FERTILIZATION International journal

    H FUNAHASHI, TC CANTLEY, TT STUMPF, SL TERLOUW, BN DAY

    BIOLOGY OF REPRODUCTION   51 ( 4 )   633 - 639   1994.10

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  • DEVELOPMENTAL ABILITY OF PORCINE OOCYTES MATURED AND FERTILIZED IN-VITRO

    H FUNAHASHI, TT STUMPF, SL TERLOUW, TC CANTLEY, A RIEKE, BN DAY

    THERIOGENOLOGY   41 ( 7 )   1425 - 1433   1994.5

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  • IN-VITRO DEVELOPMENT OF IN VITRO-MATURED PORCINE OOCYTES FOLLOWING CHEMICAL ACTIVATION OR IN-VITRO FERTILIZATION

    H FUNAHASHI, TC CANTLEY, TT STUMPF, SL TERLOUW, BN DAY

    BIOLOGY OF REPRODUCTION   50 ( 5 )   1072 - 1077   1994.5

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  • DIFFERENT HORMONAL REQUIREMENTS OF PIG OOCYTE-CUMULUS COMPLEXES DURING MATURATION IN-VITRO International journal

    H FUNAHASHI, T CANTLEY, BN DAY

    JOURNAL OF REPRODUCTION AND FERTILITY   101 ( 1 )   159 - 165   1994.5

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  • DEVELOPMENTAL ABILITY OF PORCINE OOCYTES MATURED AND FERTILIZED IN-VITRO International journal

    H FUNAHASHI, TT STUMPF, SL TERLOUW, TC CANTLEY, A RIEKE, BN DAY

    THERIOGENOLOGY   41 ( 7 )   1425 - 1433   1994.5

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  • IN-VITRO DEVELOPMENT OF IN VITRO-MATURED PORCINE OOCYTES FOLLOWING CHEMICAL ACTIVATION OR IN-VITRO FERTILIZATION International journal

    H FUNAHASHI, TC CANTLEY, TT STUMPF, SL TERLOUW, BN DAY

    BIOLOGY OF REPRODUCTION   50 ( 5 )   1072 - 1077   1994.5

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  • DIFFERENT HORMONAL REQUIREMENTS OF PIG OOCYTE-CUMULUS COMPLEXES DURING MATURATION IN-VITRO

    H FUNAHASHI, T CANTLEY, BN DAY

    JOURNAL OF REPRODUCTION AND FERTILITY   101 ( 1 )   159 - 165   1994.5

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  • DEVELOPMENT OF RAT ONE-CELL EMBRYOS IN A CHEMICALLY-DEFINED MEDIUM - EFFECTS OF GLUCOSE, PHOSPHATE AND OSMOLARITY

    K MIYOSHI, H FUNAHASHI, K OKUDA, K NIWA

    JOURNAL OF REPRODUCTION AND FERTILITY   100 ( 1 )   21 - 26   1994.1

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  • DEVELOPMENT OF RAT ONE-CELL EMBRYOS IN A CHEMICALLY-DEFINED MEDIUM - EFFECTS OF GLUCOSE, PHOSPHATE AND OSMOLARITY

    K MIYOSHI, H FUNAHASHI, K OKUDA, K NIWA

    JOURNAL OF REPRODUCTION AND FERTILITY   100 ( 1 )   21 - 26   1994.1

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  • Effects of extracellular potassium on the meiotic and cytoplasmic maturation of porcine oocytes.(共著)

    Journal of Animal Science   72 ( Supplement 1 )   71   1994

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  • HISTONE-H1 KINASE (H1K) ACTIVITY AND GLUTATHIONE (GSH) CONTENT OF PORCINE OOCYTES DURING PRONUCLEAR FORMATION AFTER IN-VITRO FERTILIZATION

    H FUNAHASHI, TT STUMPF, TC CANTLEY, NH KIM, BN DAY

    BIOLOGY OF REPRODUCTION   50 ( Supplement 1 )   128 - 128   1994

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  • Histone H1 kinase activity after electrical activation of in vitro matured porcine oocytes.(共著)

    Journal of Animal Science   72 ( Supplement 1 )   74   1994

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  • Effects of extracellular potassium on the meiotic and cytoplasmic maturation of porcine oocytes.(共著)

    Journal of Animal Science   72 ( Supplement 1 )   71   1994

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  • Effects of NaCl in maturation media on intracellular glutathione concentration and cytoplasmic maturation of porcine oocytes.(共著)

    Journal of Animal Science   72 ( Supplement 1 )   75   1994

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  • MICROTUBULE ORGANIZATION OF PORCINE OOCYTES DURING IN-VITRO FERTILIZATION

    NH KIM, H FUNAHASHI, TT STUMPF, BN DAY

    BIOLOGY OF REPRODUCTION   50 ( Supplement 1 )   146 - 146   1994

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  • 受精卵あるいは卵子輸送装置.(共著)

    特許公報   特公平6-94401   1994

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  • MICROTUBULE ORGANIZATION OF PORCINE OOCYTES DURING IN-VITRO FERTILIZATION

    NH KIM, H FUNAHASHI, TT STUMPF, BN DAY

    BIOLOGY OF REPRODUCTION   50 ( Supplement 1 )   146 - 146   1994

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  • Effects of NaCl in maturation media on intracellular glutathione concentration and cytoplasmic maturation of porcine oocytes.(共著)

    Journal of Animal Science   72 ( Supplement 1 )   75   1994

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  • In vitro maturation/in vitro fertilization of porcine oocytes.(共著)

    Proceeding for Annual Meeting of the Society for Theriogenology   206 - 214   1994

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  • HISTONE-H1 KINASE (H1K) ACTIVITY AND GLUTATHIONE (GSH) CONTENT OF PORCINE OOCYTES DURING PRONUCLEAR FORMATION AFTER IN-VITRO FERTILIZATION

    H FUNAHASHI, TT STUMPF, TC CANTLEY, NH KIM, BN DAY

    BIOLOGY OF REPRODUCTION   50 ( Supplement 1 )   128 - 128   1994

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  • Effects of sodium chloride concentration in maturation media on histone H1 kinase(H1K)activity and cytoplasmic maturation of porcine oocytes.(共著)

    Journal of Reproduction and Fertility   72 ( Abstract Series 13 )   38   1994

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  • Histone H1 kinase activity after electrical activation of in vitro matured porcine oocytes.(共著)

    Journal of Animal Science   72 ( Supplement 1 )   74   1994

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  • EFFECTS OF ELECTRICAL-STIMULATION BEFORE OR AFTER IN-VITRO FERTILIZATION ON SPERM PENETRATION AND PRONUCLEAR FORMATION OF PIG OOCYTES

    H FUNAHASHI, TT STUMPF, SL TERLOUW, BN DAY

    MOLECULAR REPRODUCTION AND DEVELOPMENT   36 ( 3 )   361 - 367   1993.11

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  • EFFECTS OF ELECTRICAL-STIMULATION BEFORE OR AFTER IN-VITRO FERTILIZATION ON SPERM PENETRATION AND PRONUCLEAR FORMATION OF PIG OOCYTES International journal

    H FUNAHASHI, TT STUMPF, SL TERLOUW, BN DAY

    MOLECULAR REPRODUCTION AND DEVELOPMENT   36 ( 3 )   361 - 367   1993.11

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  • EFFECTS OF FOLLICULAR-FLUID AT FERTILIZATION IN-VITRO ON SPERM PENETRATION IN PIG OOCYTES International journal

    H FUNAHASHI, BN DAY

    JOURNAL OF REPRODUCTION AND FERTILITY   99 ( 1 )   97 - 103   1993.9

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  • EFFECTS OF FOLLICULAR-FLUID AT FERTILIZATION IN-VITRO ON SPERM PENETRATION IN PIG OOCYTES

    H FUNAHASHI, BN DAY

    JOURNAL OF REPRODUCTION AND FERTILITY   99 ( 1 )   97 - 103   1993.9

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  • EFFECTS OF THE DURATION OF EXPOSURE TO HORMONE SUPPLEMENTS ON CYTOPLASMIC MATURATION OF PIG OOCYTES INVITRO International journal

    H FUNAHASHI, BN DAY

    JOURNAL OF REPRODUCTION AND FERTILITY   98 ( 1 )   179 - 185   1993.5

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  • EFFECTS OF THE DURATION OF EXPOSURE TO HORMONE SUPPLEMENTS ON CYTOPLASMIC MATURATION OF PIG OOCYTES INVITRO

    H FUNAHASHI, BN DAY

    JOURNAL OF REPRODUCTION AND FERTILITY   98 ( 1 )   179 - 185   1993.5

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  • EFFECTS OF DIFFERENT SERUM SUPPLEMENTS IN MATURATION MEDIUM ON MEIOTIC CYTOPLASMIC MATURATION OF PIG OOCYTES International journal

    H FUNAHASHI, BN DAY

    THERIOGENOLOGY   39 ( 4 )   965 - 973   1993.4

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  • EFFECTS OF DIFFERENT SERUM SUPPLEMENTS IN MATURATION MEDIUM ON MEIOTIC CYTOPLASMIC MATURATION OF PIG OOCYTES

    H FUNAHASHI, BN DAY

    THERIOGENOLOGY   39 ( 4 )   965 - 973   1993.4

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  • GLUCOSE REQUIREMENT AT DIFFERENT DEVELOPMENTAL STAGES OF INVITRO FERTILIZED BOVINE EMBRYOS CULTURED IN SEMI-DEFINED MEDIUM

    JH KIM, H FUNAHASHI, K NIWA, K OKUDA

    THERIOGENOLOGY   39 ( 4 )   875 - 886   1993.4

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  • GLUCOSE REQUIREMENT AT DIFFERENT DEVELOPMENTAL STAGES OF INVITRO FERTILIZED BOVINE EMBRYOS CULTURED IN SEMI-DEFINED MEDIUM International journal

    JH KIM, H FUNAHASHI, K NIWA, K OKUDA

    THERIOGENOLOGY   39 ( 4 )   875 - 886   1993.4

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  • Assessment of porcine oocytes using brilliant cresyl blue.(共著)

    Theriogenology   39 ( 1 )   214   1993

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  • Cytoplasmic maturation and in vitro development of porcine oocytes matured in Whitten's medium and fertilized in vitro.(共著)

    Fourth International Conference on Pig Reproduction   36   1993

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  • Developmental ability of porcine oocytes matured and fertilized in vitro.(共著)

    Fourth International Conference on Pig Reproduction   23   1993

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  • Effects of hormonal conditions during last 20 h of in vitro maturation on cytoplasmic maturation and vitro fertilization of pig oocytes.(共著)

    Journal of Animal Science   71 ( Supplement 1 )   70   1993

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  • Pig oocyte activation and processing of transplanted nuclei.(共著)

    39 ( 1 )   329   1993

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  • Electrical stimulation of pig pig oocytes in the presence or absence of calcium and magnesium.(共著)

    Fourth International Conference on Pig Reproduction   42   1993

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  • Processing of nuclei transplanted into in vitro matured porcine oocytes.(共著)

    Theriogenology   39 ( 1 )   322   1993

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  • Effects of artificial activation before in vitro fertilization on sperm penetration and pronuclear formation in pig oocytes.(共著)

    Theriogenology   39 ( 1 )   222   1993

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  • ラット前核期胚の体外発生におよぼすリン酸およびグルコ-スの影響.(共著)

    三好和睦, 丹羽こう二, 奥田潔, 舟橋弘晃

    家畜繁殖学会講演要旨   39 ( 5 )   a13   1993

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  • Electrical stimulation of pig pig oocytes in the presence or absence of calcium and magnesium.(共著)

    Fourth International Conference on Pig Reproduction   42   1993

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  • Cytoplasmic maturation and in vitro development of porcine oocytes matured in Whitten's medium and fertilized in vitro.(共著)

    Fourth International Conference on Pig Reproduction   36   1993

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  • Developmental ability of porcine oocytes matured and fertilized in vitro.(共著)

    Fourth International Conference on Pig Reproduction   23   1993

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  • Premature chromosomal condensation of nuclei transplanted into in vitro matured porcine oocytes.(共著)

    Biology of Reproduction   48 ( Supplement 1 )   175   1993

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  • Pronuclear formation and in vitro development folowing artificial activation of pig oocytes matured different culture media.(共著)

    Biology of Reproduction   48 ( Supplement 1 )   171   1993

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  • Effects of hormonal conditions during last 20 h of in vitro maturation on cytoplasmic maturation and vitro fertilization of pig oocytes.(共著)

    Journal of Animal Science   71 ( Supplement 1 )   70   1993

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  • Pig oocyte activation and processing of transplanted nuclei.(共著)

    39 ( 1 )   329   1993

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  • Processing of nuclei transplanted into in vitro matured porcine oocytes.(共著)

    Theriogenology   39 ( 1 )   322   1993

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  • Effects of artificial activation before in vitro fertilization on sperm penetration and pronuclear formation in pig oocytes.(共著)

    Theriogenology   39 ( 1 )   222   1993

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  • Premature chromosomal condensation of nuclei transplanted into in vitro matured porcine oocytes.(共著)

    Biology of Reproduction   48 ( Supplement 1 )   175   1993

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  • Pronuclear formation and in vitro development folowing artificial activation of pig oocytes matured different culture media.(共著)

    Biology of Reproduction   48 ( Supplement 1 )   171   1993

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  • Assessment of porcine oocytes using brilliant cresyl blue

    ERICSSON S

    Theriogenology   39 ( 1 )   214 - 214   1993

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  • FERTILIZATION AND EARLY CLEAVAGE INVITRO OF AGING BOVINE OOCYTES AFTER MATURATION IN CULTURE International journal

    RC CHIAN, H NAKAHARA, K NIWA, H FUNAHASHI

    THERIOGENOLOGY   37 ( 3 )   665 - 672   1992.3

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  • FERTILIZATION AND EARLY CLEAVAGE INVITRO OF AGING BOVINE OOCYTES AFTER MATURATION IN CULTURE

    RC CHIAN, H NAKAHARA, K NIWA, H FUNAHASHI

    THERIOGENOLOGY   37 ( 3 )   665 - 672   1992.3

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  • 体外受精由来和牛胚の凍結融解後の生存性ならびに移植・分娩成績について.(共著)

    北海道牛受精卵移植研究会会報   ( 11 )   19 - 21   1992

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  • Developmental abilities of mouse and rat pronuclear eggs following interspecific pronuclear and cytoplasmic transplantation.(共著)

    Journal of Reproduction and Development   38 ( 3 )   179 - 183   1992

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  • Developmental Abilities of Rat and Mouse Pronuclear Eggs Following Interspecific Nuclear or Cytoplasmic Transplantation

    Hiroaki Funahashp, Koji Niwa

    Journal of Reproduction and Development   38 ( 3 )   179 - 183   1992

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  • 牛体外受精卵の初期発生におよぼすグルコースの影響

    金宗興, 舟橋弘晃, 伊賀浩輔, 丹羽こう二

    日本畜産学会大会講演要旨   85th   1992

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  • Study on repeated superovulation treatment of a cow using a corpus luteum hormone preparation.

    青柳敬人, 武富敏郎, 板倉はつえ, 亀山賢次, 舟橋弘晃, 武田哲男, 鬼原辰夫

    JA全農飼料畜産中央研究所試験研究報告   ( 20(1991) )   1992

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  • Experiment on transplantation of frozen in vitro fertilized bovine ova obtained under a complete in vitro culture system.

    青柳敬人, 武富敏郎, 舟橋弘晃

    JA全農飼料畜産中央研究所試験研究報告   ( 20(1991) )   1992

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  • Experiment on production of in vitro fertilized bovine ova with donor identification.

    青柳敬人, 舟橋弘晃, 武田哲男

    JA全農飼料畜産中央研究所試験研究報告   ( 20(1991) )   1992

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  • 1ST CLEAVAGE OF ENUCLEATED RAT EGGS FOLLOWING TRANSPLANTATION OF KARYOPLAST REMOVED FROM PRONUCLEAR EGGS STORED AT 2-DEGREES-C TO 6-DEGREES-C FOR VARIOUS DURATIONS

    K NIWA, H FUNAHASHI, K OHMAE, M KATTOH

    THERIOGENOLOGY   36 ( 3 )   411 - 417   1991.9

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  • 1ST CLEAVAGE OF ENUCLEATED RAT EGGS FOLLOWING TRANSPLANTATION OF KARYOPLAST REMOVED FROM PRONUCLEAR EGGS STORED AT 2-DEGREES-C TO 6-DEGREES-C FOR VARIOUS DURATIONS

    K NIWA, H FUNAHASHI, K OHMAE, M KATTOH

    THERIOGENOLOGY   36 ( 3 )   411 - 417   1991.9

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  • DEVELOPMENTAL CAPACITY OF BOVINE OOCYTES COLLECTED FROM OVARIES OF INDIVIDUAL HEIFERS AND FERTILIZED INVITRO

    H FUNAHASHI, Y AOYAGI, T TAKEDA, T ONIHARA

    THERIOGENOLOGY   36 ( 3 )   427 - 434   1991.9

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  • DEVELOPMENTAL CAPACITY OF BOVINE OOCYTES COLLECTED FROM OVARIES OF INDIVIDUAL HEIFERS AND FERTILIZED INVITRO International journal

    H FUNAHASHI, Y AOYAGI, T TAKEDA, T ONIHARA

    THERIOGENOLOGY   36 ( 3 )   427 - 434   1991.9

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  • 黄体ホルモン製剤を用いた黒毛和種牛の反復過剰排卵処理に関する検討.(共著)

    北海道牛受精卵移植研究会会報   ( 10 )   19 - 21   1991

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  • ラット前核期胚および桑実期胚の低温保存におよぼすショ糖の影響.(共著)

    家畜繁殖学雑誌   37 ( 4 )   a21   1991

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  • Experiment on intergeneric muclear transplantation between early-stage embryos of rats and mice.

    舟橋弘晃, 武田哲男, 丹羽こう二

    JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

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  • Experiment on trasplantaion of bovine embryos produced by in vitro fertilization those were developed under a complete in vitro culture system.

    舟橋弘晃, 武富敏郎

    JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

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  • Study on nuclear transplantation in cattle.

    亀山賢次, 青柳敬人, 舟橋弘晃, 武田哲男, 武富敏郎, 板倉はつえ

    JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

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  • Study n a low-cost narcotic method for pig.

    武富敏郎, 亀山賢次, 舟橋弘晃, 板倉はつえ

    JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

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  • Experiment on developemtn in vivo of rat and mouse embryos produced by intergeneric nuclear transplantation.

    舟橋弘晃, 武田哲男, 丹羽こう二

    JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

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  • Experiment on transplantation of frozen bovine embryo splits filled in artificial zona pellucida.

    武田哲男, 青柳敬人, 亀山賢次, 武富敏郎, 舟橋弘晃, 板倉はつえ, 鬼原辰夫

    JA全農飼料畜産中央研究所試験研究報告   ( 19(1990) )   1991

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  • ラット2-細胞期胚における電気的割球融合

    舟橋 弘晃, 丹羽 皓二

    家畜繁殖学雑誌   36 ( 2 )   114 - 119   1990.6

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  • Electric fusion of 2-cell rat embryo balastomeres.

    FUNAHASHI Hiroaki, NIWA Koji

    Journal of Reproduction and Development   36 ( 2 )   114 - 119   1990

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    Language：English   Publisher：THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT  

    Two-cell rat embryos were exposed to one or a train of two electric pulses with different field strengths and durations. It was difficult to induce blastomere fusion with a field strength of 1.0 kV/cm of 50μs duration: only 1 (7%) of 15 treated embryos fused after either one or a train of two pulses. The rate of fusion reached a maximum (80-100%) with 1.5-2.0 kV/cm and with 100-200μs duration. While the fusion rates were improved when the embryos were treated with a train of two pulses of 1.0 kV/cm for 100-200 μs, little effect was observed with 1.5 and 2.0 kV/cm. With 1.0 and 1.5 kV/cm, the fusion times were shortened when the embryos were exposed to a train of two pulses of 50 to 200 μs duration; no marked shortening of the time by the increased number of pulses was observed with 2.0 kV/cm. When fused embryos were transferred into pseudopregnant females and recovered 3 days later, 15 (83%), 12 (80%) and 9 (64%) of recovered embryos, which were originally submitted to 1.0, 1.5 and 2.0 kV/cm of field strengths each for 150 μs, respectively, had developed to the morula-blastocyst stage. There were no significant differences in the rates of development among these different field strengths.

    DOI： 10.1262/jrd1977.36.114

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  • 牛体外受精卵の凍結保存におよぼす凍結保護物質の影響.(共著)

    舟橋弘晃, 板倉はつえ, 武田哲男, 鬼原辰夫

    第83回日本畜産学会大会講演要旨   83rd   15   1990

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  • 平成元年度野外実証試験について.

    第15回農協畜産技術研究会講演要旨   103 - 107   1990

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  • Study of on cryoprotectant materials for split embryos of cattle and mouse.

    武田哲男, 亀山賢次, 武富敏郎, 舟橋弘晃, 板倉はつえ

    JA全農飼料畜産中央研究所試験研究報告   ( 18(1989) )   1990

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  • Study on cryoprotectant materials for split bovine embryos.

    武田哲男, 武富敏郎, 舟橋弘晃, 板倉はつえ

    JA全農飼料畜産中央研究所試験研究報告   ( 18(1989) )   1990

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  • Experiment on transfer of porcine embryos after short-term preservation.

    亀山賢次, 武富敏郎, 板倉はつえ, 舟橋弘晃

    JA全農飼料畜産中央研究所試験研究報告   ( 18(1989) )   1990

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  • Surgical techniques for recovering and transplanting porcine embryos.

    武富敏郎, 立花文夫, 舟橋弘晃, 武田哲男, 鈴木正, 兼子よう子

    JA全農飼料畜産中央研究所試験研究報告   16 ( 1 )   493 - 501   1989

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  • ラットおよびマウス1細胞期胚の属間核置換と置換卵の発生能.(共著)

    舟橋 弘晃

    第82回日本畜産学会大会講演要旨   82回   20 - 20   1989

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    Language：Japanese   Publisher：(公社)日本畜産学会  

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  • Method of in vitro culture of an in vitro fertilized bovine ova.

    舟橋弘晃, 武富敏郎

    JA全農飼料畜産中央研究所試験研究報告   17 ( 1 )   396 - 406   1989

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  • Method of culturing a porcine embryo for 24 hours or longer.

    亀山賢次, 武富敏郎, 舟橋弘晃, 武田哲男, 板倉はつえ

    JA全農飼料畜産中央研究所試験研究報告   17 ( 1 )   390 - 395   1989

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  • Cryoprotectants used in frozen preservation of in vitro fertilized bovine ova.

    舟橋弘晃, 武富敏郎, 亀山賢次, 板倉はつえ, 武田哲男, 鬼原辰夫

    JA全農飼料畜産中央研究所試験研究報告   17 ( 1 )   381 - 389   1989

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  • Transplantation of bovine embryos produced by in vitro fertilization of ova and cultured under a variety of condition.

    舟橋弘晃, 武富敏郎, 武田哲男, 鬼原辰夫

    JA全農飼料畜産中央研究所試験研究報告   17 ( 1 )   371 - 380   1989

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  • Non-surgical techniques for transplanting frozen-thawed bovine embryos.

    武富敏郎, 鈴木正, 舟橋弘晃, 武田哲男, 立花文夫, 兼子よう子

    JA全農飼料畜産中央研究所試験研究報告   16 ( 1 )   486 - 492   1989

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  • 体外受精技術を利用した牛受精卵移植の現状と問題点.

    第14回農協畜産技術研究会講演要旨   64 - 69   1989

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  • Transplantation of porcine embryos after a longdistance transport.

    武富敏郎, 武田哲男, 舟橋弘晃, 板倉はつえ, 中谷正史, 普川一雄

    JA全農飼料畜産中央研究所試験研究報告   17 ( 1 )   365 - 370   1989

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  • Influences of heparin and caffeine on in vitro fertilization of ova and subsequent development of embryos in cattle.

    舟橋弘晃, 武富敏郎, 武田哲男, 鬼原辰夫

    JA全農飼料畜産中央研究所試験研究報告   16 ( 1 )   507 - 513   1989

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  • Influences of FSH (folicle stimulating hormone) and HCG (human chorionic gonadotrophin) on in vitro maturation of follicular oocytes.

    舟橋弘晃, 武富敏郎, 武田哲男, 鬼原辰夫

    JA全農飼料畜産中央研究所試験研究報告   16 ( 1 )   502 - 506   1989

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  • Development of rat eggs with pronuclei transplanted by electrofusion.

    舟橋弘晃, 丹羽こう二

    家畜繁殖学雑誌   34 ( 3 )   133 - 137   1988

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  • 凍結・融解した牛体外受精卵の非手術的移植後の受胎能.(共著)

    家畜繁殖学雑誌   34 ( 4 )   末   1988

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  • カフェインとヘパリンの添加培地で得られた牛体外受精卵の発生能.(共著)

    家畜繁殖学雑誌   34 ( 4 )   末   1988

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  • ラットにおける前核置換卵の移植による産子の生産.(共著)

    家畜繁殖学雑誌   33 ( 4 )   末   1987

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  • ラット2細胞期胚割球の電気的融合条件.(共著)

    第79回日本畜産学会大会講演要旨   11   1987

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  • ラット前核期卵における核移植に関する基礎的研究.(共著)

    哺乳動物卵子研究会誌   2 ( 1 )   75 - 78   1985

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  • The basic study on unclear transplantation in rat zygote by HVJ-mediated cell fusion karyoplast.

    Journal of Mammalian Ova Research   2 ( 1 )   75 - 78   1985

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  • The basic study on uuclear transplantation in rat zygote by HVJ-mediated cell fusion karyoplast.

    Journal of Mammalian Ova Research   2 ( 1 )   75 - 78   1985

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  • ラット前核期卵における雄性前核の紫外線スポット照射による不活化と作出された雌性二倍体胚の発育性について.(共著)

    日本畜産学会関西支部会報   101,23   1985

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  • マウス排卵卵子への精巣精子、精巣上体精子の顕微注入.(共著)

    日本不妊学会雑誌   31 ( 1 )   160   1985

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  • Nuclear transplantation into pronuclear-stage rat eggs : Enucleation of eggs by UV-irradiation.

    Journal of Mammalian Ova Research   1 ( 1 )   85 - 89   1984

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  • Nuclear transplantation in pronuclear stage rat eggs : Inactivation of nucleus by micro-spot UV-irradiation.(共著) International Symposium on Mammalian Reproduction and Early

    Develelopment, Abstracts   48   1984

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  • ラット前核期卵におよぼす前核への紫外線照射の影響.(共著)

    日本畜産学会関西支部会報   ( 98 )   30   1984

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  • ラット前核期卵の核移植:特に紫外線照射による非外科的核除去について.(共著)

    哺乳動物卵子研究会誌   1 ( 1 )   85 - 89   1984

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  • Nuclear transplantation in pronuclear stage rat eggs : Inactivation of nucleus by micro-spot UV-irradiation.(共著)

    International Symposium on Mammalian Reproduction and Early Develelopment, Abstracts   48   1984

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  • ラット受精卵の体外培養について -血清を中心とした培養液要素についての検討.(共著)

    日本不妊学会雑誌   28 ( 2 )   148   1982

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Industrial property rights

  • 運動性良好な精子採取装置

    松浦 宏治, 舟橋 弘晃, 古市 卓也

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    Applicant：国立大学法人 岡山大学

    Application no：特願2012-081396  Date applied：2012.3.30

    Announcement no：特開2012-213395  Date announced：2012.11.8

    Patent/Registration no：特許第5877512号  Date registered：2016.2.5 

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  • 光学的観察用チャンバー及び試料の光学的観察方法、並びに下側透明板の製造方法

    松浦 宏治, 舟橋 弘晃

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    Applicant：国立大学法人 岡山大学

    Application no：特願2011-523592  Date applied：2010.6.24

    Publication no：WO2011-010525  Date published：2011127

    Patent/Registration no：特許第5610313号  Date registered：2014.9.12 

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  • 卵細胞の培養装置

    成瀬 恵治, 舟橋 弘晃, 石田 敬雄, 松浦 宏治, 原 鐵晃

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    Applicant：国立大学法人 岡山大学

    Application no：特願2007-194968  Date applied：2007.7.26

    Announcement no：特開2009-027973  Date announced：2009.2.12

    Patent/Registration no：特許第5083952号  Date registered：2012.9.14 

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  • 再構築受精卵の作製方法及びそれを用いたトランスジェニック胚の作製方法

    小西 正人, 青柳 敬人, 舟橋 弘晃, 岡部 勝

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    Applicant：全国農業協同組合連合会

    Application no：特願2000-232041  Date applied：2000.7.31

    Announcement no：特開2001-103867  Date announced：2001.4.17

    Patent/Registration no：特許第4845073号  Date registered：2011.10.21 

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  • アデノシンおよび受精促進ペプチドを利用した効率的な体外受精卵生産方法

    舟橋 弘晃

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    Applicant：岡山大学長

    Application no：特願2000-070752  Date applied：2000.3.14

    Announcement no：特開2001-251990  Date announced：2001.9.18

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  • 受精卵あるいは卵子輸送装置

    舟橋 弘晃, 井田 脩三

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    Applicant：全国農業協同組合連合会

    Application no：特願平2-404910  Date applied：1990.12.21

    Announcement no：特開平5-310501  Date announced：1993.11.22

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Awards

  • 日本繁殖生物学会賞(島村賞)

    1998  

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    Country：Japan

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Research Projects

  • Creation of mammalian oocytes with high fertility by spatiotemporal regulation of the function of mitochondria

    Grant number：18H02328  2018.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    FUNAHASHI Hiroaki

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    Grant amount：\17290000 （ Direct expense: \13300000 、 Indirect expense：\3990000 ）

    The aim of this study was to generate oocytes with high developmental competence by regulating the mitochondrial (mt) DNA copy number in the ooplasm. Porcine ooplasmic mtDNA copy number did not change during in vitro maturation (IVM) and was lower in oocytes from small than medium follicles. Drp1 (mt mitogen) was reduced during early development, and its inhibition impaired the mt distribution in oocytes and early developmental competence. Overexpression of PGC1alpha (mt synthesis-related genes) resulted in increased reactive oxygen species and mtDNA copy number reduction in oocytes, and reduced IVM rate and early developmental competence. The autophagic potential of oocytes varied greatly during IVM and early development, and differed significantly by follicle diameter of origin. Attempts to introduce photosensitizers and peptide/protein molecular tools into oocytes have not resulted in significant improvement.

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  • Development of functional photosensitizing peptide for photocontrol of biofunctions

    Grant number：18H02090  2018.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Ohtsuki Takashi

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    Grant amount：\17160000 （ Direct expense: \13200000 、 Indirect expense：\3960000 ）

    In this study, we developed a molecular tool combining a photosensitizer and a peptide/protein (functional photosensitizing peptide; FPP) to establish a technology for the spatiotemporal control and observation of cellular functions by light. First, we elucidated the effects of the amino acid sequence near the photosensitizers to support FPP design. Furthermore, by using this technology, we established a method for light-directed cytosolic delivery of two different RNAs, clarified the cell cycle dependence of apoptosis photo-triggered using a FPP, and succeeded in photoinduced RNA delivery into a single cell in mouse embryos.

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  • Cryopreservation of ovarian tissue

    Grant number：16K11089  2016.04 - 2019.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Motohashi Hideyuki

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    Grant amount：\4680000 （ Direct expense: \3600000 、 Indirect expense：\1080000 ）

    The cancer treatments in young women such as anti-cancer drugs and radiation can affect fertility by several biologic systems. The purpose of this study was to develop a novel cryopreservation method for ovarian tissue. In this study, an improved vitrification for ovarian tissue resulted in survival and subsequent development in vivo and oocyte maturation in vitro after cryopreservation.

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  • Characteristic analyses of boar seminal gel and the application

    Grant number：24658236  2012.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    FUNAHASHI Hiroaki

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    Grant amount：\2990000 （ Direct expense: \2300000 、 Indirect expense：\690000 ）

    Characteristic analyses of seminal gel in ejaculated boar semen were analysed. The gel could be dissolved in both conditions of severe acidic and alkaline conditions, whereas it is stable around neutral conditions. Chemotaxis of polymorphonuclear leukocytes for seminal gel was not observed. Preparation of sperm capsule by mixing frozen-dried seminal gel with boar semen or washed sperm allowed survival and release of sperm from the capsule. Chemical analyses of seminal gel demonstrated that the gel is o-glycan-rich. Analyses of sugar chains and peptides showed that seminal gel contains specific sugar chains and protein. These information may have possibilities to contribute in wide fields including biomedical area.

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  • Investigation for effects of blood coagulation factor XI deficiency on productivity to the genetic improvement of Japanese beef cattle

    Grant number：23380166  2011.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    KUNIEDA Tetsuo, TSUJI Takehito, IBI Takayuki, FUNAHASHI Hiroaki, ACOSTA Tomas

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    Grant amount：\18200000 （ Direct expense: \14000000 、 Indirect expense：\4200000 ）

    Blood coagulation factor XI deficiency is a hereditary disorder observed in Japanese Black cattle. While the clinical condition of this disorder is mild, the economical impact of this disorder is apprehended to be serious because of the remarkably high allelic frequency of the mutant allele in the population of Japanese Black cattle. We, therefore, investigated the effect of the factor XI deficiency on defects in pregnancy and delivery and carcass traits using large samples of Japanese Black cattle. As a result, we found that affected animals of factor XI deficiency have a tendencies for increased frequencies of abortion and stillborn, but no effect on the condition of calf and carcass traits. These findings will be useful to future breeding of Japanese Black cattle.

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  • Immunological regulation of the female reproductive tract to establish an efficient new artificial insemination system in mammals

    Grant number：20380154  2008 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    FUNAHASHI Hiroaki, KONDOU Yasuhiro

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    Grant amount：\9360000 （ Direct expense: \7200000 、 Indirect expense：\2160000 ）

    In the female reproductive tracts, immunological system exists to remove sperm from the tract rapidly after mating or artificial insemination. In the present study, we could make clear that both phagocytotic and chemotactic activities of bovine and porcine polymorphonuclear leukocytes (PMNs), which mainly perform in the system, are stimulated in the presence of fresh serum and decreased in the presence of caffeine, heparin, and seminal plasma. Furthermore, PMNs phagocytize equally both active motile and dead sperm.

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  • Development of a transient co-incubation IVF system in the pig

    Grant number：16580230  2004 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    FUNAHASHI Hiroaki

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    Grant amount：\3600000 （ Direct expense: \3600000 ）

    This research project was undertaken to develop a transient-co-incubation IVF system in the pig, in order to obtain an efficient normal monospermic sperm penetration. Obtained sights are as follows :
    (A)Effect of pretreatment of spermatozoa with adenosine and a transient gamete co-incubation with caffeine
    A new transient co-incubation IVF system, in which denuded oocytes are co-cultured with spermatozoa in medium containing caffeine for 5 to 30 min and then continuing the culture in caffeine-free medium, will reduce the incidence of polyspermic penetration. Preincubation of fresh spermatozoa with adenosine before the transient co-incubation IVF can also improve the monospermic penetration.
    (B)Effective regulation of sperm capacitation during transient IVF period
    The presence of beta-mercaptoethanol (bME) prevents induction of sperm capacitation even in the presence of caffeine, whereas H_2O_2 induces sperm capacitation and the spontaneous acrosome reaction.
    (C)Regulation of oxidative stress of gametes during transient co-incubation
    Supplementation with bME during IVF procedures, except during a transient co-culture period of gametes in the presence of caffeine, has a beneficial effect in maintaining the function of gametes, the incidence of normal fertilization and, consequently, the quality of IVF embryos.
    (D)Selection of antioxidants to improve the function of extended boar semen stored at a low temperature
    Glutathione and cysteine can improve the viability and functional status of boar spermatozoa during liquid preservation and boar spermatozoa penetrated in vitro even after preservation in the presence of cysteine at 10℃ for 29 days.
    (E)Development of an IVM system for the transient IVF system
    Although cytoplasmic maturation in porcine oocytes pretreated with roscovitine for 48 h did not equal that of control oocytes in the current IVM system, those stored oocytes can matured, fertilized and developed in vitro to the blastocyst stage.

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  • Development of an efficient transgenic method bu using GFP-reporter gene and nuclear transfer

    Grant number：10556065  1998 - 2001

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    FUNAHASHI Hiroaki, AOYAGI Yoshito, NIWA Koji, OKABE Masaru

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    Grant amount：\12300000 （ Direct expense: \12300000 ）

    The effect of timing of microinjection of DNA constructs on the efficiency of transgenic embryo production and improved efficiency and quality through combining EGFP as a reporter gene with nuclear transfer techniques were examined. From 12-24 h after insemination, constructs of pCXNeo-EGFP were microinjected into a pronucleus of bovine IVM-IVF zygotes. Due to difficulty in visualizing the pronucleus, the incidence of successful injection of linear DNA into pronucleus was higher when injected from 20 to 24 h, as compared with an early period from 12 to 16 h after insemination. However, developmental competence of DNA-injected zygotes and the EGFP-expression rate were not different among the variously-timed microinjections. A majority of the embryos expressing EGFP signal were mosaic. Following nuclear transfer of blastomeres expressing EGFP, 4.5 % of morulae that developed from the NT-eggs had a strong EGFP signal in all live blastomeres. In other embryos, EGFP signal had been lost. When cells derived from the EGFP-positive NT morulae were subcultured, all the cells expressed strong EGFP signal at the second passage and demonstrated neomycin resistance. These results show that transient expression of non-integrated EGFP appears frequently in EGFP-positive bovine embryos and that additional selection of EGFP-positive morulae after nuclear transfer of EGFP-positive blastomeres would facilitate selection of transgenic embryos.
    Efficient methods to produce porcine zygotes in vitro for the microinjection of DNA constructs were also developed. Replacement of caffeine with adenosine or fertilization promoting peptide, which induced capacitation but prevented acrosome reaction of boar spermatozoa, reduced the incidence of polyspermic penetration of porcine oocytes without any reduction in penetration.

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  • Packaging of Mammalian Paternal Genome and Male Pronuclear Formation

    Grant number：09660303  1997 - 1998

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    FUNAHASHI Hiroaki

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    Grant amount：\3800000 （ Direct expense: \3800000 ）

    DNA-stability and thiol-disulfide status of rat sperm nuclei was observed during maturation in the epididymis and during penetration to the oocytes in vivo. When spermatids and spermatozoa were stained with acridine orange (AO) after fixation with acetic alcohol, the ratio of red/green fluorescence under a confocal microscope decreased until caput epididymis. On the other hand, when spermatozoa were labeled with rnBBr the fluorescence intensities gradually decreased during transportation of spermatozoa from testis through epididymis. The ratio of red/green AO fluorescence increased during penetration of zona pellucida and sperm decondensation. When spermatozoa in the perivitelline space were labeled with mBBr and examined fluorescence was detected. These results demonstrate that DNA stability against acid appears to be ahead of the oxidization of protamine during sperm maturation in the epididymis and that an initial event of the unpackaging process in rat genome begins during or just after zona-penetration but before penetration into the ooplasm.
    The contents of glutathione and other thiols in rat eggs were examined during sperm penetration and pronuclear formation by high-performance liquid chromatography with fluorescence detection. Reduced glutathione (GSH) content was higher in unfertilized oocytes and penetrated eggs with a decondensed sperm nucleus than eggs at the pronuclear stage. The content of oxidized glutathione (GSSG) was not different among experimental groups. The ratio of GSSG/GSH was not different during fertilization. Although reduced cysteinylglycine content did not changed among experimental groups, oxidized form of cysteinylglycine increased between sperm decondensation and pronuclear formation. Low contents of cystine were detected during fertilization but cysteine and gamma-glutamylcysteine were not detected in any treatment groups. These results demonstrate that GSH content in rat eggs decreases between sperm decondensation and pronuclear formation, probably due to the increased activity of gamma-glutamyl transpeptidase.

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  • 哺乳動物精子の受精能獲得機構に関する研究

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    Grant type：Competitive

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  • 哺乳動物受精卵における雄性前核形成のメカニズムに関する研究

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    Grant type：Competitive

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  • 哺乳動物生殖細胞の体外生産及び操作に関する研究

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    Grant type：Competitive

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  • Study on the capacitation of mammalian spermatogoa

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    Grant type：Competitive

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  • Study on the mechanisn of male pronuclear formation in mammalian zygotes.

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    Grant type：Competitive

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  • Study on in vitro production and manipulation of mammalian gametes.

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    Grant type：Competitive

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Academic Activities

  • International Embryo Transfer Society Foundation, trustee

    Role(s)：Planning, management, etc.

    International Embryo Transfer Society  2010.1 - 2015.1

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    Type：Academic society, research group, etc. 

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