Faculty Profiles - Shitamukai Atsunori

Papers - Shitamukai Atsunori

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Evidence for antagonistic regulation of cell growth by the calcineurin and high osmolarity glycerol pathways in Saccharomyces cerevisiae. Reviewed International journal

Atsunori Shitamukai, Dai Hirata, Shinya Sonobe, Tokichi Miyakawa

The Journal of biological chemistry 279 ( 5 ) 3651 - 61 2004.1

Authorship：Lead author Language：English

Because Ca(2+) signaling of budding yeast, through the activation of calcineurin and the Mpk1/Slt2 mitogen-activated protein kinase cascade, performs redundant function(s) in the events essential for growth, the simultaneous deletion of both these pathways (Delta cnb1 Delta mpk1) leads to lethality. A PTC4 cDNA that encodes a protein phosphatase belonging to the PP2C family was obtained as a high dosage suppressor of the lethality of Delta cnb1 Delta mpk1 strain. Overexpression of PTC4 led to a decrease in the high osmolarity-induced Hog1 phosphorylation, and HOG1 deletion remarkably suppressed the synthetic lethality, indicating an antagonistic role of the high osmolarity glycerol (HOG) pathway and the Ca(2+) signaling pathway in growth regulation. The calcineurin-Crz1 pathway was required for the down-regulation of the HOG pathway. Analysis of the time course of actin polarization, bud formation, and the onset of mitosis in synchronous cell cultures demonstrated that calcineurin negatively regulates actin polarization at the bud site, whereas the HOG pathway positively regulates bud formation at a later step after actin has polarized.

DOI： 10.1074/jbc.M306098200

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酵母の特殊な変異形質を利用するCa^<2+>シグナル伝達に作用する薬剤のポジティブスクリーニング Invited

下向敦範, 水沼正樹, 平田大, 高橋英俊, 宮川都吉

日本農芸化学会誌 76 ( 8 ) 734 - 735 2002.8

酵母の特殊な変異形質を利用するCa2+シグナル伝達に作用する薬剤のポジティブスクリーニング Invited

下向敦範, 水沼正樹, 平田大, 高橋英俊, 宮川都吉

化学と生物(日本農芸化学会誌) 76 38 - 39 2002

酵母を使った生理活性物質の新規スクリーニング法 Invited

平田大, 下向敦範, 水沼正樹, 宮川都吉

生物工学会誌 79 ( 3 ) 79 - 79 2001

A positive screening for drugs that specifically inhibit the Ca2+-signaling activity on the basis of the growth promoting effect on a yeast mutant with a peculiar phenotype. Reviewed International journal

A Shitamukai, M Mizunuma, D Hirata, H Takahashi, T Miyakawa

Bioscience, biotechnology, and biochemistry 64 ( 9 ) 1942 - 6 2000.9

Authorship：Lead author Language：English

An inappropriate activation of a signaling pathway in yeast often has a deleterious physiological effect and causes various defects, including growth defects. In a certain genetic background (deltazds1) of Saccharomyces cerevisiae, the cell-cycle progression in G2 is specifically blocked in the medium with CaCl2 by the hyperactivation of the Ca2+-signaling pathways. Here, we developed a novel drug screening procedure designed to detect the active compounds that specifically attenuate the Ca2+-signaling activity on the basis of the ability to abrogate the growth defect of the cells suffering from the hyperactivated Ca2+ signal. Using known calcineurin inhibitors as model compounds, we have established the screening conditions for the drugs that suppress the Ca2+-induced growth inhibition. An indicator strain with an increased drug sensitivity was constructed with a syr1/erg3 null mutation.

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