2022/07/02 更新

写真a

サイショウ ダイスケ
最相 大輔
SAISHO Daisuke
所属
資源植物科学研究所 准教授
職名
准教授
外部リンク

学位

  • 博士(農学) ( 東京大学 )

研究キーワード

  • 植物育種学

  • 遺伝資源

  • オオムギ

  • 栽培進化

  • 遺伝的多様性

  • Plant Molecular Genetics

  • 植物分子遺伝学

研究分野

  • 環境・農学 / 遺伝育種科学

所属学協会

委員歴

  • 日本育種学会 運営委員(庶務)(2014-2018)  

    2014年 - 2018年   

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  • Japanese Society of Breeding  

    2014年 - 2018年   

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論文

  • Genetic Elucidation for Response of Flowering Time to Ambient Temperatures in Asian Rice Cultivars

    Kiyosumi Hori, Daisuke Saisho, Kazufumi Nagata, Yasunori Nonoue, Yukiko Uehara-Yamaguchi, Asaka Kanatani, Koka Shu, Takashi Hirayama, Jun-ichi Yonemaru, Shuichi Fukuoka, Keiichi Mochida

    International Journal of Molecular Sciences   22 ( 3 )   1024 - 1024   2021年1月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    Climate resilience of crops is critical for global food security. Understanding the genetic basis of plant responses to ambient environmental changes is key to developing resilient crops. To detect genetic factors that set flowering time according to seasonal temperature conditions, we evaluated differences of flowering time over years by using chromosome segment substitution lines (CSSLs) derived from japonica rice cultivars “Koshihikari” × “Khao Nam Jen”, each with different robustness of flowering time to environmental fluctuations. The difference of flowering times in 9 years’ field tests was large in “Khao Nam Jen” (36.7 days) but small in “Koshihikari” (9.9 days). Part of this difference was explained by two QTLs. A CSSL with a “Khao Nam Jen” segment on chromosome 11 showed 28.0 days’ difference; this QTL would encode a novel flowering-time gene. Another CSSL with a segment from “Khao Nam Jen” in the region around Hd16 on chromosome 3 showed 23.4 days” difference. A near-isogenic line (NIL) for Hd16 showed 21.6 days’ difference, suggesting Hd16 as a candidate for this QTL. RNA-seq analysis showed differential expression of several flowering-time genes between early and late flowering seasons. Low-temperature treatment at panicle initiation stage significantly delayed flowering in the CSSL and NIL compared with “Koshihikari”. Our results unravel the molecular control of flowering time under ambient temperature fluctuations.

    DOI: 10.3390/ijms22031024

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  • Breeding for low cadmium barley by introgression of a Sukkula-like transposable element

    Gui Jie Lei, Miho Fujii-Kashino, De Zhi Wu, Hiroshi Hisano, Daisuke Saisho, Fenglin Deng, Naoki Yamaji, Kazuhiro Sato, Fang-Jie Zhao, Jian Feng Ma

    Nature Food   1 ( 8 )   489 - 499   2020年8月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1038/s43016-020-0130-x

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    その他リンク: http://www.nature.com/articles/s43016-020-0130-x

  • Life-course monitoring of endogenous phytohormone levels under field conditions reveals diversity of physiological states among barley accessions. 査読

    Takashi Hirayama, Daisuke Saisho, Takakazu Matsuura, Satoshi Okada, Kotaro Takahagi, Asaka Kanatani, Jun Ito, Hiroyuki Tsuji, Yoko Ikeda, Keiichi Mochida

    Plant & cell physiology   61 ( 8 )   1438 - 1448   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Agronomically important traits often develop during the later stages of crop growth as consequences of various plant-environment interactions. Therefore, the temporal physiological states that change and accumulate during the crop's life course can significantly affect the eventual phenotypic differences in agronomic traits among crop varieties. Thus, to improve productivity, it is important to elucidate the associations between temporal physiological responses during the growth of different crop varieties and their agronomic traits. However, data representing the dynamics and diversity of physiological states in plants grown under field conditions is sparse. In this study, we quantified the endogenous levels of five phytohormones-auxin, cytokinins, abscisic acid, jasmonate, and salicylic acid-in the leaves of eight diverse barley (Hordeum vulgare) accessions grown under field conditions sampled weekly over their life course to assess the ongoing fluctuations in hormone levels in the different accessions under field growth conditions. Notably, we observed enormous changes over time in the development-related plant hormones, such as auxin and cytokinins. Using 3' RNA-seq-based transcriptome data from the same samples, we investigated the expression of barley genes orthologous to known hormone-related genes of Arabidopsis throughout the life course. These data illustrated the dynamics and diversity of the physiological states of these field-grown barley accessions. Together our findings provide new insights into plant-environment interaction, highlighting that there is cultivar diversity in physiological responses during growth under field conditions.

    DOI: 10.1093/pcp/pcaa046

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  • Training instance segmentation neural network with synthetic datasets for crop seed phenotyping. 国際誌

    Yosuke Toda, Fumio Okura, Jun Ito, Satoshi Okada, Toshinori Kinoshita, Hiroyuki Tsuji, Daisuke Saisho

    Communications biology   3 ( 1 )   173 - 173   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In order to train the neural network for plant phenotyping, a sufficient amount of training data must be prepared, which requires time-consuming manual data annotation process that often becomes the limiting step. Here, we show that an instance segmentation neural network aimed to phenotype the barley seed morphology of various cultivars, can be sufficiently trained purely by a synthetically generated dataset. Our attempt is based on the concept of domain randomization, where a large amount of image is generated by randomly orienting the seed object to a virtual canvas. The trained model showed 96% recall and 95% average Precision against the real-world test dataset. We show that our approach is effective also for various crops including rice, lettuce, oat, and wheat. Constructing and utilizing such synthetic data can be a powerful method to alleviate human labor costs for deploying deep learning-based analysis in the agricultural domain.

    DOI: 10.1038/s42003-020-0905-5

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  • Retrotransposon Insertion and DNA Methylation Regulate Aluminum Tolerance in European Barley Accessions. 査読 国際誌

    Miho Kashino-Fujii, Kengo Yokosho, Naoki Yamaji, Miki Yamane, Daisuke Saisho, Kazuhiro Sato, Jian Feng Ma

    Plant physiology   178 ( 2 )   716 - 727   2018年10月

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    記述言語:英語  

    Aluminum (Al) toxicity is a major stress factor limiting crop productivity in acid soil. Although there is great genotypic variation in tolerance to Al toxicity, the underlying molecular mechanisms are poorly understood. Here, we report that, in barley (Hordeum vulgare), the fourth largest cereal crop produced in the world, both retrotransposon insertion and DNA methylation are involved in regulating differential Al tolerance. HvAACT1 is a major gene responsible for citrate secretion from the roots for external detoxification of Al. A multiretrotransposon-like (MRL) sequence insertion at least 15.3 kb in length was detected in the upstream genomic region of HvAACT1 that displayed promoter activity and significantly enhanced HvAACT1 expression, especially in the root tips of Al-tolerant accessions. Furthermore, in a number of accessions with low levels of HvAACT1 expression, this MRL insertion was present but highly methylated. Geographical analysis showed that accessions with this MRL insertion are distributed mainly in European areas with acid soils. Two wild barley accessions were found to possess this MRL insertion, but with a high degree of methylation. These results indicate that the MRL insertion and its degree of DNA methylation influence HvAACT1 expression and that demethylation of this MRL insertion, which facilitates adaptation to acid soils, occurred following barley domestication. Moreover, our results indicate that barley accessions in East Asia and Europe have developed independent but equivalent strategies to withstand Al toxicity in acid soils.

    DOI: 10.1104/pp.18.00651

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  • Both retrotransposon insertion and demethylation regulate aluminum tolerance of European barley for postdomestication expansion to acid soil.

    Fujii-Kashino, M, Yamaji, N, Yamane, M, Saisho, D, Sato, K, Ma, J. F

    Plant Physiology   2018年

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  • Crop improvement using life cycle datasets acquired under field conditions 査読

    Keiichi Mochida, Daisuke Saisho, Takashi Hirayama

    Frontiers in Plant Science   6 ( September )   2015年9月

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    記述言語:英語   出版者・発行元:Frontiers Research Foundation  

    Crops are exposed to various environmental stresses in the field throughout their life cycle. Modern plant science has provided remarkable insights into the molecular networks of plant stress responses in laboratory conditions, but the responses of different crops to environmental stresses in the field need to be elucidated. Recent advances in omics analytical techniques and information technology have enabled us to integrate data from a spectrum of physiological metrics of field crops. The interdisciplinary efforts of plant science and data science enable us to explore factors that affect crop productivity and identify stress tolerance-related genes and alleles. Here, we describe recent advances in technologies that are key components for data driven crop design, such as population genomics, chronological omics analyses, and computer-aided molecular network prediction. Integration of the outcomes from these technologies will accelerate our understanding of crop phenology under practical field situations and identify key characteristics to represent crop stress status. These elements would help us to genetically engineer “designed crops” to prevent yield shortfalls because of environmental fluctuations due to future climate change.

    DOI: 10.3389/fpls.2015.00740

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    その他リンク: http://orcid.org/0000-0002-3868-2380

  • ~水 土壌 作物~ 環境ストレス突破の分子生理基盤と育種との融合

    最相 大輔, 堀江 智明, 木下 俊則, 馬 建鋒

    育種学研究   17 ( 2 )   71 - 76   2015年

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    記述言語:日本語   出版者・発行元:日本育種学会  

    DOI: 10.1270/jsbbr.17.71

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    その他リンク: https://jlc.jst.go.jp/DN/JLC/20012694585?from=CiNii

  • 新しいモデル草本植物 ミナトカモジグサ (Brachypodium distachyon) 査読

    篠崎一雄持田恵一, 恩田義彦, 佐々木忠将, 氷室泰代, 最相大輔, 小林正智, 平山隆志

    植物の生長調節   50 ( 2 )   103 - 109   2015年

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    出版者・発行元:植物化学調節学会  

    DOI: 10.18978/jscrp.50.2_103

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    その他リンク: http://orcid.org/0000-0003-0484-3008

  • Phytochrome C is a key factor controlling long-day flowering in barley 査読

    Hidetaka Nishida, Daisuke Ishihara, Makoto Ishii, Takuma Kaneko, Hiroyuki Kawahigashi, Yukari Akashi, Daisuke Saisho, Katsunori Tanaka, Hirokazu Handa, Kazuyoshi Takeda, Kenji Kato

    Plant Physiology   163 ( 2 )   804 - 814   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The spring-type near isogenic line (NIL) of the winter-type barley (Hordeum vulgare ssp. vulgare) var. Hayakiso 2 (HK2) was developed by introducing VERNALIZATION-H1 (Vrn-H1) for spring growth habit from the spring-type var. Indo Omugi. Contrary to expectations, the spring-type NIL flowered later than winter-type HK2. This phenotypic difference was controlled by a single gene, which cosegregated only with phytochrome C (HvPhyC) among three candidates around the Vrn-H1 region (Vrn-H1, HvPhyC, and CASEIN KINASE IIα), indicating that HvPhyC was the most likely candidate gene. Compared with the late-flowering allele HvPhyC-l from the NIL, the early-flowering allele HvPhyC-e from HK2 had a single nucleotide polymorphism T1139C in exon 1, which caused a nonsynonymous amino acid substitution of phenylalanine at position 380 by serine in the functionally essential GAF (39, 59-cyclic-GMP phosphodiesterase, adenylate cyclase, formate hydrogen lyase activator protein) domain. Functional assay using a rice (Oryza sativa) phyA phyC double mutant line showed that both of the HvPhyC alleles are functional, but HvPhyC-e may have a hyperfunction. Expression analysis using NILs carrying HvPhyC-e and HvPhyC-l (NIL [HvPhyC-e] and NIL [HvPhyC-l], respectively) showed that HvPhyC-e up-regulated only the flowering promoter FLOWERING LOCUS T1 by bypassing the circadian clock genes and flowering integrator CONSTANS1 under a long photoperiod. Consistent with the up-regulation, NIL (HvPhyC-e) flowered earlier than NIL (HvPhyC-l) under long photoperiods. These results implied that HvPhyC is a key factor to control long-day flowering directly. © 2013 American Society of Plant Biologists. All Rights Reserved.

    DOI: 10.1104/pp.113.222570

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  • Duplicate polyphenol oxidase genes on barley chromosome 2H and their functional differentiation in the phenol reaction of spikes and grains 査読

    Shin Taketa, Kanako Matsuki, Satoko Amano, Daisuke Saisho, Eiko Himi, Naoki Shitsukawa, Takahisa Yuo, Kazuhiko Noda, Kazuyoshi Takeda

    Journal of Experimental Botany   61 ( 14 )   3983 - 3993   2010年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Polyphenol oxidases (PPOs) are copper-containing metalloenzymes encoded in the nucleus and transported into the plastids. Reportedly, PPOs cause time-dependent discoloration (browning) of end-products of wheat and barley, which impairs their appearance quality. For this study, two barley PPO homologues were amplified using PCR with a primer pair designed in the copper binding domains of the wheat PPO genes. The full-lengths of the respective PPO genes were cloned using a BAC library, inverse-PCR, and 3′-RACE. Linkage analysis showed that the polymorphisms in PPO1 and PPO2 co-segregated with the phenol reaction phenotype of awns. Subsequent RT-PCR experiments showed that PPO1 was expressed in hulls and awns, and that PPO2 was expressed in the caryopses. Allelic variation of PPO1 and PPO2 was analysed in 51 barley accessions with the negative phenol reaction of awns. In PPO1, amino acid substitutions of five types affecting functionally important motif(s) or C-terminal region(s) were identified in 40 of the 51 accessions tested. In PPO2, only one mutant allele with a precocious stop codon resulting from an 8 bp insertion in the first exon was found in three of the 51 accessions tested. These observations demonstrate that PPO1 is the major determinant controlling the phenol reaction of awns. Comparisons of PPO1 single mutants and the PPO1PPO2 double mutant indicate that PPO2 controls the phenol reaction in the crease on the ventral side of caryopses. An insertion of a hAT-family transposon in the promoter region of PPO2 may be responsible for different expression patterns of the duplicate PPO genes in barley. © 2010 The Author(s).

    DOI: 10.1093/jxb/erq211

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  • Allelic variation of row type gene Vrs1 in barley and implication of the functional divergence 査読

    Daisuke Saisho, Mohammad Pourkheirandish, Hiroyuki Kanamori, Takashi Matsumoto, Takao Komatsuda

    BREEDING SCIENCE   59 ( 5 )   621 - 628   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC BREEDING  

    Domesticated barleys produce either two- or six- rowed spikes, whereas their immediate wild ancestor, wild barley, is monomorphic for the two-rowed type. The six-rowed spike is a recessive character, conditioned by a major gene at the vrs1 locus. The wild-type (two-rowed) gene includes a homeodomain-leucine zipper I (HD-Zip 1) sequence (HvHox1). The correspondence between peptide sequence and some spike variants was studied by re-sequencing the HvHox1 sequence across a large sample of both wild and domesticated accessions.

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▼全件表示

書籍等出版物

  • コラム1 QTL解析の実践:押さえておきたい3つのキーワード「連続分布」「分散」「遺伝率」

    文一総合出版  2010年 

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MISC

  • オオムギ根圏の共生微生物の単離と同定

    木代勝元, 最相大輔, 山下純, 山地直樹, 山本敏央, 門田有希, 持田恵一, 中川智行, 谷明生

    日本農芸化学会大会講演要旨集(Web)   2021   2021年

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  • スペイン由来のスペルトコムギを用いて同定した2A染色体の根毛長QTL

    岡野なつみ, 後藤稜, 加藤拓, 最相大輔, 加藤鎌司, 三浦秀穂, 谷昌幸, 大西一光

    育種学研究   22   2020年

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  • 醸造用オオムギの一穂粒数増大に向けた同質遺伝子系統の育成

    最相大輔, 轟貴智, 原口雄飛, 甲斐浩臣, 半田裕一, 佐藤和広

    育種学研究   21   33   2019年9月

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    記述言語:日本語  

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  • 水稲品種Khao Nam Jenに由来する日長非依存的な出穂期QTLの検出

    堀清純, 永田和史, 福岡修一, 最相大輔, 平山隆志, 持田恵一

    育種学研究   20   2018年

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  • 圃場環境下における野生オオムギと栽培オオムギの生長段階と植物ホルモン動態の季節変動

    最相大輔, 松浦恭和, 池田陽子, 森泉, 持田恵一, 持田恵一, 平山隆志

    育種学研究   19   269   2017年3月

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    記述言語:日本語  

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  • Salt tolerance during germination and seedling growth of wild wheat Aegilops tauschii and its impact on the species range expansion

    Daisuke Saisho, Shigeo Takumi, Yoshihiro Matsuoka

    SCIENTIFIC REPORTS   6   2016年12月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Adaptation to edaphic stress may have a key role in plant species range expansion. Aegilops tauschii Coss., the common wheat's D-genome progenitor native to the Transcaucasus-Middle East region, is a good model to study the relationships between soil salinity and plant distributions: one of its intraspecific sublineages, TauL1b, drove the long-distance eastward expansion of this species range reaching semi-arid-central Asia. Salt tolerance during germination and seedling growth was evaluated in 206 Ae. tauschii accessions by treating seeds with NaCl solutions differing in concentrations. Differences in natural variation patterns were analyzed between sublineages and associated with natural edaphic condition variables, and then compared with reproductive trait variation patterns. The natural variations observed in NaCl-induced-stress tolerance had clear geographic and genetic structure. Seedling growth significantly increased in the TauL1b accessions that were collected from salt-affected soil habitats, whereas germinability did not. Principal component analysis suggested that the NaCl-induced-stress tolerances and reproductive traits might have had a similar degree of influence on Ae. tauschii's eastward range expansion. Adaptation to salt-affected soils through increased seedling growth was an important factor for the species' successful colonization of the semi-arid central Asian habitats. TauL1b accessions might provide useful genetic resources for salt-tolerant wheat breeds.

    DOI: 10.1038/srep38554

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  • Analysis of single nucleotide polymorphisms based on RNA sequencing data of diverse bio-geographical accessions in barley

    Kotaro Takahagi, Yukiko Uehara-Yamaguchi, Takuhiro Yoshida, Tetsuya Sakurai, Kazuo Shinozaki, Keiichi Mochida, Daisuke Saisho

    SCIENTIFIC REPORTS   6   2016年9月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Barley is one of the founder crops of Old world agriculture and has become the fourth most important cereal worldwide. Information on genome-scale DNA polymorphisms allows elucidating the evolutionary history behind domestication, as well as discovering and isolating useful genes for molecular breeding. Deep transcriptome sequencing enables the exploration of sequence variations in transcribed sequences; such analysis is particularly useful for species with large and complex genomes, such as barley. In this study, we performed RNA sequencing of 20 barley accessions, comprising representatives of several biogeographic regions and a wild ancestor. We identified 38,729 to 79,949 SNPs in the 19 domesticated accessions and 55,403 SNPs in the wild barley and revealed their genome-wide distribution using a reference genome. Genome-scale comparisons among accessions showed a clear differentiation between oriental and occidental barley populations. The results based on population structure analyses provide genome-scale properties of sub-populations grouped to oriental, occidental and marginal groups in barley. Our findings suggest that the oriental population of domesticated barley has genomic variations distinct from those in occidental groups, which might have contributed to barley's domestication.

    DOI: 10.1038/srep33199

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  • 野生オオムギと栽培オオムギにおける胚乳細胞壁の厚さに関する解析

    最相大輔, 松島良, 本庄三恵, 八杉公基, 永野惇, 永野惇, 高萩航太郎, 高萩航太郎, 持田恵一, 持田恵一, 持田恵一, 武田真, 坂本亘

    育種学研究   18   2016年

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  • オオムギ発芽時耐塩性のQTL解析

    伊藤大樹, 最相大輔, 本庄三恵, 八杉公基, 永野惇, 高萩航太郎, 持田恵一, 佐藤和広

    育種学研究   17   236   2015年3月

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    記述言語:日本語  

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  • Acquisition of aluminium tolerance by modification of a single gene in barley. 国際誌

    Miho Fujii, Kengo Yokosho, Naoki Yamaji, Daisuke Saisho, Miki Yamane, Hirokazu Takahashi, Kazuhiro Sato, Mikio Nakazono, Jian Feng Ma

    Nature communications   3   713 - 713   2012年3月

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    記述言語:英語  

    Originating from the Fertile Crescent in the Middle East, barley has now been cultivated widely on different soil types including acid soils, where aluminium toxicity is a major limiting factor. Here we show that the adaptation of barley to acid soils is achieved by the modification of a single gene (HvAACT1) encoding a citrate transporter. We find that the primary function of this protein is to release citrate from the root pericycle cells to the xylem to facilitate the translocation of iron from roots to shoots. However, a 1-kb insertion in the upstream of the HvAACT1 coding region occurring only in the Al-tolerant accessions, enhances its expression and alters the location of expression to the root tips. The altered HvAACT1 has an important role in detoxifying aluminium by secreting citrate to the rhizosphere. Thus, the insertion of a 1-kb sequence in the HvAACT1 upstream enables barley to adapt to acidic soils.

    DOI: 10.1038/ncomms1726

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  • 11-11 オオムギのアルミニウム耐性機構の獲得(11.植物の有害元素,2012年度鳥取大会)

    柏野 美帆, 横正 健剛, 山地 直樹, 最相 大輔, 山根 美樹, 高橋 宏和, 佐藤 和広, 中園 幹生, 馬 建鋒

    日本土壌肥料学会講演要旨集   58 ( 0 )   2012年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • オオムギ春播性遺伝子Vrn-H1の近傍に見出された新規日長反応性遺伝子HvPhyC

    西田英隆, 金古卓磨, 石原大輔, 川東広幸, 半田裕一, 石井誠, 最相大輔, 明石由香利, 武田和義, 加藤鎌司

    育種学研究   14   2012年

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  • 栽培オオムギにおける春化要求性の自然変異:高度秋播性は東アジアに偏在する

    最相大輔, 石井誠, 堀清純, 佐藤和広

    育種学研究   13   80   2011年9月

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    記述言語:日本語  

    J-GLOBAL

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  • Widespread Endogenization of Genome Sequences of Non-Retroviral RNA Viruses into Plant Genomes

    Sotaro Chiba, Hideki Kondo, Akio Tani, Daisuke Saisho, Wataru Sakamoto, Satoko Kanematsu, Nobuhiro Suzuki

    PLOS PATHOGENS   7 ( 7 )   e1002146   2011年7月

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    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Non-retroviral RNA virus sequences (NRVSs) have been found in the chromosomes of vertebrates and fungi, but not plants. Here we report similarly endogenized NRVSs derived from plus-, negative-, and double-stranded RNA viruses in plant chromosomes. These sequences were found by searching public genomic sequence databases, and, importantly, most NRVSs were subsequently detected by direct molecular analyses of plant DNAs. The most widespread NRVSs were related to the coat protein (CP) genes of the family Partitiviridae which have bisegmented dsRNA genomes, and included plant-and fungus-infecting members. The CP of a novel fungal virus (Rosellinia necatrix partitivirus 2, RnPV2) had the greatest sequence similarity to Arabidopsis thaliana ILR2, which is thought to regulate the activities of the phytohormone auxin, indole-3-acetic acid (IAA). Furthermore, partitivirus CP-like sequences much more closely related to plant partitiviruses than to RnPV2 were identified in a wide range of plant species. In addition, the nucleocapsid protein genes of cytorhabdoviruses and varicosaviruses were found in species of over 9 plant families, including Brassicaceae and Solanaceae. A replicase-like sequence of a betaflexivirus was identified in the cucumber genome. The pattern of occurrence of NRVSs and the phylogenetic analyses of NRVSs and related viruses indicate that multiple independent integrations into many plant lineages may have occurred. For example, one of the NRVSs was retained in Ar. thaliana but not in Ar. lyrata or other related Camelina species, whereas another NRVS displayed the reverse pattern. Our study has shown that single-and double-stranded RNA viral sequences are widespread in plant genomes, and shows the potential of genome integrated NRVSs to contribute to resolve unclear phylogenetic relationships of plant species.

    DOI: 10.1371/journal.ppat.1002146

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  • Natural variation of barley vernalization requirements: implication of quantitative variation of winter growth habit as an adaptive trait in East Asia.

    Daisuke Saisho, Makoto Ishii, Kiyosumi Hori, Kazuhiro Sato

    Plant & cell physiology   52 ( 5 )   775 - 84   2011年5月

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    記述言語:英語  

    In many temperate plant species, prolonged cold treatment, known as vernalization, is one of the most critical steps in the transition from the vegetative to the reproductive stage. In contrast to recent advances in understanding the molecular basis of vernalization in Arabidopsis non-vernalization mutants or the spring growth habits of cereal crops such as wheat and barley, natural variations in winter growth habits and their geographic distribution are poorly understood. We analyzed varietal variation and the geographic distribution of the degree of vernalization requirements in germplasms of domesticated barley and wild barley collections. We found a biased geographic distribution of vernalization requirements in domesticated barley: Western regions were strongly associated with a higher degree of spring growth habits, and the extreme winter growth habits were localized to Far Eastern regions including China, Korea and Japan. Both wild accessions and domesticated landraces, the regions of distribution of which overlapped each other, mainly belonged to the moderate class of winter growth habit. As a result of quantitative evaluations performed in this study, we provide evidence that the variation in the degree of winter growth habit in recombinant inbred lines was controlled by quantitative trait loci including three vernalization genes (VRN1, VRN2 and VRN3) that account for 37.9% of the variation in vernalization requirements, with unknown gene(s) explaining the remaining two-thirds of the variation. This evidence implied that the Far Eastern accessions might be a genetically differentiated group derived for an evolutionary reason, resulting in their greater tendency towards a winter growth habit.

    DOI: 10.1093/pcp/pcr046

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  • Barley: Emergence as a New Research Material of Crop Science

    Daisuke Saisho, Kazuyoshi Takeda

    PLANT AND CELL PHYSIOLOGY   52 ( 5 )   724 - 727   2011年5月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    DOI: 10.1093/pcp/pcr049

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  • Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos

    Kazuhide Rikiishi, Daisuke Saisho, Kazuyoshi Takeda

    Proceedings of the 10th international barley genetics symposium   679 - 686   2010年

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  • TriMEDB: A database to integrate transcribed markers and facilitate genetic studies of the tribe Triticeae

    Keiichi Mochida, Daisuke Saisho, Takuhiro Yoshida, Tetsuya Sakurai, Kazuo Shinozaki

    BMC PLANT BIOLOGY   8   72   2008年6月

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    記述言語:英語   出版者・発行元:BIOMED CENTRAL LTD  

    Background: The recent rapid accumulation of sequence resources of various crop species ensures an improvement in the genetics approach, including quantitative trait loci (QTL) analysis as well as the holistic population analysis and association mapping of natural variations. Because the tribe Triticeae includes important cereals such as wheat and barley, integration of information on the genetic markers in these crops should effectively accelerate map-based genetic studies on Triticeae species and lead to the discovery of key loci involved in plant productivity, which can contribute to sustainable food production. Therefore, informatics applications and a semantic knowledgebase of genome-wide markers are required for the integration of information on and further development of genetic markers in wheat and barley in order to advance conventional marker-assisted genetic analyses and population genomics of Triticeae species.
    Description: The Triticeae mapped expressed sequence tag (EST) database (TriMEDB) provides information, along with various annotations, regarding mapped cDNA markers that are related to barley and their homologues in wheat. The current version of TriMEDB provides map-location data for barley and wheat ESTs that were retrieved from 3 published barley linkage maps (the barley single nucleotide polymorphism database of the Scottish Crop Research Institute, the barley transcript map of Leibniz Institute of Plant Genetics and Crop Plant Research, and HarvEST barley ver. 1.63) and 1 diploid wheat map. These data were imported to CMap to allow the visualization of the map positions of the ESTs and interrelationships of these ESTs with public gene models and representative cDNA sequences. The retrieved cDNA sequences corresponding to each EST marker were assigned to the rice genome to predict an exon-intron structure. Furthermore, to generate a unique set of EST markers in Triticeae plants among the public domain, 3472 markers were assembled to form 2737 unique marker groups as contigs. These contigs were applied for pairwise comparison among linkage maps obtained from different EST map resources.
    Conclusion: TriMEDB provides information regarding transcribed genetic markers and functions as a semantic knowledgebase offering an informatics facility for the acceleration of QTL analysis and for population genetics studies of Triticeae.

    DOI: 10.1186/1471-2229-8-72

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  • Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos

    Kazuhide Rikiishi, Daisuke Saisho, Kazuyoshi Takeda

    BREEDING SCIENCE   58 ( 2 )   149 - 155   2008年6月

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    記述言語:英語   出版者・発行元:JAPANESE SOC BREEDING  

    Barley includes semi-dwarf varieties, called uzu, which are localized in parts of southwestern Japan, the Southern Korea peninsula, and coastal areas of China. The uzu phenotype possesses dark green leaves and short coleoptiles, awns, and panicles. It is controlled by a single recessive gene: uzu. Uzu results from a mutation in the brassinosteroid receptor kinase gene (HvBR11). Brassinosteroid synergistically acts with auxin on plant morphology, which is an important plant hormone for tissue culture. For this study, tissue Culture traits, including callus growth and shoot regeneration capability, were examined in F-2 populations derived from crosses between normal and uzu lines, and in isogenic lines for the uzu gene. The uzu genotype shows a lower percentage of shoot regeneration than the normal genotype in F, populations and isogenic lines. The uzu gene negatively affects shoot regeneration. No significant differences were Found In callus growth capability between uzu and normal genotypes. Uzu isogenic lines show higher sensitivity to exogenous auxin for callus initiation than normal lines, when immature embryos were incubated on media supplemented with several concentrations of 2,4-D under culture at higher temperature (25 degrees C). Tissue Culture traits of uzu might be regulated through cross-talk between brassinosteroid and auxin.

    DOI: 10.1270/jsbbs.58.149

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  • Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway. 国際誌

    Shin Taketa, Satoko Amano, Yasuhiro Tsujino, Tomohiko Sato, Daisuke Saisho, Katsuyuki Kakeda, Mika Nomura, Toshisada Suzuki, Takashi Matsumoto, Kazuhiro Sato, Hiroyuki Kanamori, Shinji Kawasaki, Kazuyoshi Takeda

    Proceedings of the National Academy of Sciences of the United States of America   105 ( 10 )   4062 - 7   2008年3月

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    記述言語:英語  

    In contrast to other cereals, typical barley cultivars have caryopses with adhering hulls at maturity, known as covered (hulled) barley. However, a few barley cultivars are a free-threshing variant called naked (hulless) barley. The covered/naked caryopsis is controlled by a single locus (nud) on chromosome arm 7HL. On the basis of positional cloning, we concluded that an ethylene response factor (ERF) family transcription factor gene controls the covered/naked caryopsis phenotype. This conclusion was validated by (i) fixation of the 17-kb deletion harboring the ERF gene among all 100 naked cultivars studied; (ii) two x-ray-induced nud alleles with a DNA lesion at a different site, each affecting the putative functional motif; and (iii) gene expression strictly localized to the testa. Available results indicate the monophyletic origin of naked barley. The Nud gene has homology to the Arabidopsis WIN1/SHN1 transcription factor gene, whose deduced function is control of a lipid biosynthesis pathway. Staining with a lipophilic dye (Sudan black B) detected a lipid layer on the pericarp epidermis only in covered barley. We infer that, in covered barley, the contact of the caryopsis surface, overlaid with lipids to the inner side of the hull, generates organ adhesion.

    DOI: 10.1073/pnas.0711034105

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  • 東アジアに局在するオオムギ半矮性系統”渦”の進化的・形態的・生理的な多様性解析

    武田和義, 力石和英, 最相大輔

    平成16年度〜平成18年度 科学研究費補助金 基盤研究B 研究成果報告書   1 - 110   2008年

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  • Molecular phylogeography of domesticated barley traces expansion of agriculture in the Old World

    Daisuke Saisho, Michael D. Purugganan

    GENETICS   177 ( 3 )   1765 - 1776   2007年11月

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    記述言語:英語   出版者・発行元:GENETICS  

    Barley (Hordeum vulgare ssp. vulgare) was first cultivated 10,500 years ago in the Fertile Crescent and is one of the founder crops of Eurasian agriculture. Phylogeographic analysis of five nuclear loci and morphological assessment of two traits in >250 domesticated barley accessions reveal that landraces found in South and East Asia are genetically distinct from those in Europe and North Africa. A Bayesian population structure assessment method indicates that barley accessions are subdivided into six clusters and that barley landraces from 10 different geographical regions of Eurasia and North Africa show distinct patterns of distribution across these clusters. Using haplotype frequency data, it appears that the Europe/North Africa landraces are most similar to the Near East population (F-ST = 0.15) as well as to wild barley (F-ST = 0.11) and are strongly differentiated from all other Asian populations (F-ST = 0.34-0.74). A neighbor-joining analysis using these F-ST estimates also supports a division between European, North African, and Near East barley types from more easterly Asian accessions. There is also differentiation in the presence of a naked caryopsis and spikelet row number between eastern and western barley accessions. The data support the differential migration of barley from two domestication events that led to the origin of barley - one in the Fertile Crescent and another farther east, possibly at the eastern edge of the Iranian Plateau - with European and North African barley largely originating from the former and much of Asian barley arising from the latter. This suggests that cultural diffusion or independent innovation is responsible for the expansion of agriculture to areas of South and East Asia during the Neolithic revolution.

    DOI: 10.1534/genetics.107.079491

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  • Construction and Characterization of a Bacterial Artificial Chromosome (BAC) Library from Japanese Malting Barley ‘Haruna Nijo’.

    Saisho, D, E. Myoraku, S. Kawasaki, K. Sato, K. Takeda

    Breed. Sci.   57 ( 1 )   29 - 38   2007年3月

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  • 草型変異から見たイネとオオムギの栽培化.

    浅野賢治, 最相大輔, 芦苅基行, 松岡信

    蛋白質 核酸 酵素   52   1931 - 1936   2007年

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  • 酸化ストレスとミトコンドリア機能.

    目黒直樹, 最相大輔, 中園幹生

    蛋白質 核酸 酵素   2005年

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  • Spontaneous brassinolide-insensitive barley mutant ‘uzu’ adapted to East Asia

    Saisho, D, K. Tanno, M. Chono, I. Honda, H. Kitano, K. Takeda

    Breed. Sci.   54 ( 4 )   409 - 416   2004年12月

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  • A semidwarf phenotype of barley uzu results from a nucleotide substitution in the gene encoding a putative brassinosteroid receptor. 国際誌

    Makiko Chono, Ichiro Honda, Haruko Zeniya, Koichi Yoneyama, Daisuke Saisho, Kazuyoshi Takeda, Suguru Takatsuto, Tsuguhiro Hoshino, Yoshiaki Watanabe

    Plant physiology   133 ( 3 )   1209 - 19   2003年11月

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    記述言語:英語  

    Brassinosteroids (BRs) play important roles throughout plant growth and development. Despite the importance of clarifying the mechanism of BR-related growth regulation in cereal crops, BR-related cereal mutants have been identified only in rice (Oryza sativa). We previously found that semidwarf barley (Hordeum vulgare) accessions carrying the "uzu" gene, called "uzu" barley in Japan, are non-responding for brassinolide (BL). We then performed chemical and molecular analyses to clarify the mechanisms of uzu dwarfism using isogenic line pairs of uzu gene. The response of the uzu line to BL was significantly lower than that of its corresponding normal line. Measurement of BRs showed that the uzu line accumulates BRs, similar to known BR-insensitive mutants. The marker synteny of rice and barley chromosomes suggests that the uzu gene may be homologous to rice D61, a rice homolog of Arabidopsis BR-insensitive 1 (BRI1), encoding a BR-receptor protein. A barley homolog of BRI1, HvBRI1, was isolated by using degenerate primers. A comparison of HvBRI1 sequences in uzu and normal barley varieties showed that the uzu phenotype is correlated with a single nucleotide substitution. This substitution results in an amino acid change at a highly conserved residue in the kinase domain of the BR-receptor protein. These results may indicate that uzu dwarfism is caused by the missense mutation in HvBRI1. The uzu gene is being introduced into all hull-less barley cultivars in Japan as an effective dwarf gene for practical use, and this is the first report about an agronomically important mutation related to BRs.

    DOI: 10.1104/pp.103.026195

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  • 渦遺伝子がオオムギ未熟胚由来カルスからの植物体再分化に及ぼす影響

    力石和英, 最相大輔, 前川雅彦, 武田和義

    育種学研究   2003年

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  • Barley uzu semi-dwarf caused by brassinosteroid insensitive mutation.

    D Saisho, M Chono, Honda, I, Y Watanabe, KI Tanno, H Kitano, K Takeda

    PLANT AND CELL PHYSIOLOGY   44   S71 - S71   2003年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

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  • オオムギ半矮性遺伝子uzu座に連鎖するマイクロサテライトマーカーの遺伝的多様性

    丹野 研一, 南角 奈美, 最相 大輔, 武田 和義

    育種学研究 = Breeding research   4   399 - 399   2002年8月

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  • 醸造用オオムギ"はるな二条"のBACライブラリー構築

    最相 大輔, 川崎 信二, 佐藤 和広, 武田 和義

    育種学研究 = Breeding research   4   269 - 269   2002年8月

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  • オオムギにおける半矮性「渦」の特性解析

    武田和義, 最相大輔, 力石和英, 北野英巳

    育種学研究   2002年

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  • The gene for alternative oxidase-2 (AOX2) from Arabidopsis thaliana consists of five exons unlike other AOX genes and is transcribed at an early stage during germination

    D Saisho, M Nakazono, KH Lee, N Tsutsumi, S Akita, A Hirai

    GENES & GENETIC SYSTEMS   76 ( 2 )   89 - 97   2001年4月

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    記述言語:英語   出版者・発行元:GENETICS SOC JAPAN  

    We investigated the expressions of genes for alternative oxidase (AOX1a, AOX1b, AOX1c and AOX2) and genes for cytochrome c oxidase (COX5b and COX6b) during germination of Arabidopsis thaliana, and examined oxygen uptakes of the alternative respiration and the cytochrome respiration in imbibed Arabidopsis seeds. A Northern blot analysis showed that AOX2 mRNA has already accumulated in dry seeds and subsequently decreased, whereas accumulation of AOX1a mRNA was less abundant from 0 hours to 48 hours after imbibition and then increased. The increase of the capacity of the alternative pathway appeared to be dependent on the expressions of both AOX2 and AOX1a. On the other hand, steady-state mRNA levels of COX5b and COX6b were gradually increased during germination, and the capacity of the cytochrome pathway was correlated with the increase of expressions of the COX genes. Antimycin A, the respiratory inhibitor, strongly increased the expression of AOX1a but had no effect on the expression of AOX2. A 5'RACE analysis showed that AOX2 consists of five exons, which is different from the case of most AOX genes identified so far. Analysis of subcellular localization of AOX2 using green fluorescent protein indicated that the AOX2 protein is imported into the mitochondria.

    DOI: 10.1266/ggs.76.89

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  • ATP synthesis inhibitors as well as respiratory inhibitors increase steady-state level of alternative oxidase mRNA in Arabidopsis thaliana

    D Saisho, M Nakazono, N Tsutsumi, A Hirai

    JOURNAL OF PLANT PHYSIOLOGY   158 ( 2 )   241 - 245   2001年2月

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    記述言語:英語   出版者・発行元:URBAN & FISCHER VERLAG  

    The oxygen uptake in mitochondria can be uncoupled from ATP synthesis by the product of a single gene, the alternative oxidase (AOX) gene, which may be involved in signal transduction from mitochondria to the nucleus. To better understand induction of this gene in higher plants, we investigated the effects of the cytochrome respiratory inhibitors and ATP synthesis inhibitors on the expression of the AOXs of Arabidopsis thaliana. A Northern blot analysis showed the AOX1a transcripts were increased by treatment of antimycin A and myxothiazol, which act on complex III of the respiratory chain, and by NaN3, which acts on complex IV. AOX1a mRNA was also strongly induced by oligomycin (F1F0-ATP synthase inhibitor) and weakly induced by 2,4-DNP (ATP synthesis uncoupler) at the maximum dose. These results indicate that not only inhibition of electron transfer but also inhibition of ATP synthesis in mitochondria induces the transcription of AOX1a.

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  • Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions

    M Nakazono, H Tsuji, YH Li, D Saisho, S Arimura, N Tsutsumi, A Hirai

    PLANT PHYSIOLOGY   124 ( 2 )   587 - 598   2000年10月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT PHYSIOLOGISTS  

    It is known that alcoholic fermentation is important for survival of plants under anaerobic conditions. Acetaldehyde, one of the intermediates of alcoholic fermentation, is not only reduced by alcohol dehydrogenase but also can be oxidized by aldehyde dehydrogenase (ALDH). To determine whether ALDH plays a role in anaerobic metabolism in rice (Oryza sativa L. cv Nipponbare), we characterized a cDNA clone encoding mitochondrial ALDH from rice (Aldh2a). Analysis of sub-cellular localization of ALDH2a protein using green fluorescent protein and an in vitro ALDH assay using protein extracts from Escherichia coli cells that overexpressed ALDH2a indicated that ALDH2a functions in the oxidation of acetaldehyde in mitochondria. A Southern-blot analysis indicated that mitochondrial ALDH is encoded by at least two genes in rice. We found that the Aldh2a mRNA was present at high levels in leaves of dark-grown seedlings, mature leaf sheaths, and panicles. It is interesting that expression of the rice Aldh2a gene, unlike the expression of the tobacco (Nicotiana tabacum) Aldh2a gene, was induced in rice seedlings by submergence. Experiments with ruthenium red, which is a blocker of Ca2+ fluxes in rice as well as maize (Zea mays), suggest that the induction of expression of Adh1 and Pdc1 by low oxygen stress is regulated by elevation of the cytosolic Ca2+ level. However, the induction of Aldh2a gene expression may not be controlled by the cytosolic Ca2+ level elevation. A possible involvement of ALDH2a in the submergence tolerance of rice is discussed.

    DOI: 10.1104/pp.124.2.587

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  • オオムギのcDNAシークエンス解析 : ビールオオムギ「はるな二条」と日本在来品種「赤神力」との比較

    最相 大輔, 佐藤 和広, 高橋 秀和, 武田 和義

    育種学研究 = Breeding research   2 ( 2 )   32 - 32   2000年9月

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  • Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation: Evidence for involvement of calcium in expression of the alternative oxidase 1a gene

    Hiroyuki Tsuji, Mikio Nakazono, Daisuke Saisho, Nobuhiro Tsutsumi, Atsushi Hirai

    FEBS Letters   471 ( 2-3 )   201 - 204   2000年4月

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    記述言語:英語  

    We investigated the effect of oxygen on the expressions of respiratory genes encoded in the nuclear and mitochondrial genomes of rice (Oryza sativa L.). Hypoxic treatment decreased the transcript levels of nuclear-encoded, but not mitochondrial-encoded respiratory genes. The effects of ruthenium red (an inhibitor of Ca2+ fluxes from organelles) and/or CaCl2 on plants under hypoxic conditions suggested that Ca2+ is a physiological transducer of a low-oxygen signaling pathway for expression of the alternative oxidase 1a gene (AOX1a), but not for expressions of genes involved in the cytochrome respiratory pathway, in rice. Copyright (C) 2000 Federation of European Biochemical Societies.

    DOI: 10.1016/S0014-5793(00)01411-3

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  • Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation : evidence for involvement of calcium in expression of the alternative oxidase 1a gene.(共著)

    FEBS Lett.   471 ( 2-3 )   201 - 204   2000年4月

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  • Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation: evidence for involvement of calcium in expression of the alternative oxidase la gene

    H Tsuji, M Nakazono, D Saisho, N Tsutsumi, A Hirai

    FEBS LETTERS   471 ( 2-3 )   201 - 204   2000年4月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    We investigated the effect of oxygen on the expressionse of respiratory genes encoded in the nuclear and mitochondrial genomes of rice (Oryza sativa L.). Hypoxic treatment decreased the transcript levels of nuclear-encoded, but not mitochondrial-encoded respiratory gents. The effects of ruthenium red (an inhibitor of Ca2+ fluxes from organelles) and/or CaCl2 on plants under hypoxic conditions suggested that Ca2+ is a physiological transducer of a low-oxygen signaling pathway for expression of the alternative oxidase 1a gene (AOX1a), but not for expressions of genes involved in the cytochrome respiratory pathway, in rice. (C) 2000 Federation of European Biochemical Societies.

    DOI: 10.1016/S0014-5793(00)01411-3

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  • 低酸素によるイネAOX1a遺伝子の発現抑制には細胞内カルシウムイオンが関与している

    辻 寛之, 中園 幹生, 最相 大輔, 堤 伸浩, 平井 篤志

    育種学研究 = Breeding research   2 ( 1 )   2000年4月

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    記述言語:日本語  

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  • Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions.(共著)

    Plant Physiol.   124 ( 2 )   587 - 598   2000年

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  • Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions

    M. Nakazono, H. Tsuji, Y. Li, D. Saisho, S. I. Arimura, N. Tsutsumi, A. Hirai

    Plant Physiology   124 ( 2 )   587 - 598   2000年

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    記述言語:英語   出版者・発行元:American Society of Plant Biologists  

    It is known that alcoholic fermentation is important for survival of plants under anaerobic conditions. Acetaldehyde, one of the intermediates of alcoholic fermentation, is not only reduced by alcohol dehydrogenase but also can be oxidized by aldehyde dehydrogenase (ALDH). To determine whether ALDH plays a role in anaerobic metabolism in rice (Oryza sativa L. cv Nipponbare), we characterized a cDNA clone encoding mitochondrial ALDH from rice (Aldh2a). Analysis of sub-cellular localization of ALDH2a protein using green fluorescent protein and an in vitro ALDH assay using protein extracts from Escherichia coli cells that overexpressed ALDH2a indicated that ALDH2a functions in the oxidation of acetaldehyde in mitochondria. A Southern-blot analysis indicated that mitochondrial ALDH is encoded by at least two genes in rice. We found that the Aldh2a mRNA was present at high levels in leaves of dark-grown seedlings, mature leaf sheaths, and panicles. It is interesting that expression of the rice Aldh2a gene, unlike the expression of the tobacco (Nicotiana tabacum) Aldh2a gene, was induced in rice seedlings by submergence. Experiments with ruthenium red, which is a blocker of Ca2+ fluxes in rice as well as maize (Zea mays), suggest that the induction of expression of Adh1 and Pdc1 by low oxygen stress is regulated by elevation of the cytosolic Ca2+ level. However, the induction of Aldh2a gene expression may not be controlled by the cytosolic Ca2+ level elevation. A possible involvement of ALDH2a in the submergence tolerance of rice is discussed.

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  • 冠水条件下でのイネミトコンドリア遺伝子の発現応答 2. アルデヒド脱水素酵素(ALDH)遺伝子の発現誘導

    中園幹生, 辻寛之, 最相大輔, 中園江, 堤伸浩, 平井篤志

    育種学研究   1   76   1999年4月

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    記述言語:日本語  

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  • 冠水条件下でのイネミトコンドリア遺伝子の発現応答 1. 呼吸系遺伝子の発現

    辻寛之, 中園幹生, 最相大輔, 堤伸浩, 平井篤志

    育種学研究   1   75   1999年4月

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    記述言語:日本語  

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  • Expression of the alternative oxidase gene is induced by treatment of rice with the bleaching herbicide norflurazon

    Daisuke Saisho, Mikio Nakazono, Nobuhiro Tsutsumi, Atsushi Hirai

    RICE GENET. NEWSLT.   1999年

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  • CYCLOHEXIMIDE INDUCES TRANSCRIPTION OF THE GENE FOR ALTERNATIVE OXIDASE IN Arabidopsis thaliana

    SAISHO Daisuke, TSUTSUMI Nobuhiro, HIRAI Atsushi, NAKAZONO Mikio

    Plant and cell physiology   39   S94 - S94   1998年5月

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  • Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature.(共著)

    GENE   203 ( 2 )   121 - 129   1997年12月

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  • Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature

    Yusuke Ito, Daisuke Saisho, Mikio Nakazono, Nobuhiro Tsutsumi, Atsushi Hirai

    Gene   203 ( 2 )   121 - 129   1997年12月

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    記述言語:英語  

    We identified two genes for alternative oxidase (AOX) from rice. One AOX gene (designated AOX1a) is located approx. 1.9 kb downstream of another AOX gene (designated AOX1b). Comparison of the genomic and cDNA sequences of the two AOX genes showed that the AOX1a gene is interrupted by three introns, as are AOX genes of other plants. On the other hand, two introns are inserted in the AOX1b gene. The predicted AOX1a and AOX1b precursor proteins consist of 332 and 335 amino acid residues, respectively. A genomic Southern hybridization analysis indicated that rice has several AOX genes other than the two tandem-arranged AOX genes. Steady-state mRNA levels of both of the genes for AOX1a and AOX1b were increased under low temperature (4°C). However, no difference in the pattern of induction of transcription between the genes for AOX1a and AOX1b was observed.

    DOI: 10.1016/S0378-1119(97)00502-7

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  • Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature. (共著)

    GENE   203 ( 2 )   121 - 129   1997年12月

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  • Characterization of the gene family for alternative oxidase from Arabidopsis thaliana.(共著)

    Plant Mol. Biol.   35 ( 5 )   585 - 596   1997年11月

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  • Characterization of the gene family for alternative oxidase from Arabidopsis thaliana

    Daisuke Saisho, Eiji Nambara, Satoshi Naito, Nobuhiro Tsutsumi, Atsushi Hirai, Mikio Nakazono

    Plant Molecular Biology   35 ( 5 )   585 - 596   1997年11月

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    記述言語:英語  

    We investigated the copy number of the gene for alternative oxidase (AOX) of Arabidopsis thaliana by amplification by PCR and Southern hybridization. These studies indicated that there are at least four copies of the AOX gene in Arabidopsis. We isolated genomic clones containing individual copies (designated as AOX1a, AOX1b, AOX1c and AOX2) of the AOX genes. Interestingly, two of the AOX genes (AOX1a and AOX1b) were located in tandem in a ca. 5 kb region on one of the chromosomes of Arabidopsis. Comparison between genomic and cDNA sequences of the four AOX genes showed that all AOX genes are divided by three introns and the positions of the introns in AOX1a, AOX1b, AOX1c and AOX2 are the same. We examined whether expression of Arabidopsis AOX genes, like the tobacco AOX1a gene, is enhanced by treatment with antimycin A, an inhibitor of complex III in the mitochondrial respiratory chain. We found that, in young plants, the amount of Arabidopsis AOX1a mRNA was dramatically increased by addition of antimycin A, while the transcription of the other three genes (AOX1b, AOX1c and AOX2) did not respond to antimycin A. Amplification by RT-PCR showed that AOX1a and AOX1c were expressed in all organs examined (flowers and buds, stems, rosette, and roots of 8-week old plants). In contrast, transcripts of AOX1b were detected only in the flowers and buds, and transcripts of AOX2 were detected mainly in stems, rosette and roots. These results suggested that transcriptions of the four genes for alternative oxidase of Arabidopsis are differentially regulated.

    DOI: 10.1023/A:1005818507743

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  • Characterization of the gene family for alternative oxidase from Arabidopsis thaliana

    Daisuke Saisho, Eiji Nambara, Satoshi Naito, Nobuhiro Tsutsumi, Atsushi Hirai, Mikio Nakazono

    Plant Molecular Biology   35 ( 5 )   585 - 596   1997年11月

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    記述言語:英語  

    We investigated the copy number of the gene for alternative oxidase (AOX) of Arabidopsis thaliana by amplification by PCR and Southern hybridization. These studies indicated that there are at least four copies of the AOX gene in Arabidopsis. We isolated genomic clones containing individual copies (designated as AOX1a, AOX1b, AOX1c and AOX2) of the AOX genes. Interestingly, two of the AOX genes (AOX1a and AOX1b) were located in tandem in a ca. 5 kb region on one of the chromosomes of Arabidopsis. Comparison between genomic and cDNA sequences of the four AOX genes showed that all AOX genes are divided by three introns and the positions of the introns in AOX1a, AOX1b, AOX1c and AOX2 are the same. We examined whether expression of Arabidopsis AOX genes, like the tobacco AOX1a gene, is enhanced by treatment with antimycin A, an inhibitor of complex III in the mitochondrial respiratory chain. We found that, in young plants, the amount of Arabidopsis AOX1a mRNA was dramatically increased by addition of antimycin A, while the transcription of the other three genes (AOX1b, AOX1c and AOX2) did not respond to antimycin A. Amplification by RT-PCR showed that AOX1a and AOX1c were expressed in all organs examined (flowers and buds, stems, rosette, and roots of 8-week old plants). In contrast, transcripts of AOX1b were detected only in the flowers and buds, and transcripts of AOX2 were detected mainly in stems, rosette and roots. These results suggested that transcriptions of the four genes for alternative oxidase of Arabidopsis are differentially regulated.

    DOI: 10.1023/A:1005818507743

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  • Two tandemly-repeated genes for alternative oxidase from Arabidopsis thaliana are differentially expressed.

    M Nakazono, D Saisho, S Naito, N Tsutsumi, A Hirai

    PLANT PHYSIOLOGY   114 ( 3 )   1014 - 1014   1997年7月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER SOC PLANT PHYSIOLOGISTS  

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  • STRUCTURE AND EXPRESSION OF THE GENE FOR THE CYANIDE-INSENSITIVE, ALTERNATIVE OXIDASE FROM Arabidopsis thaliana

    NAKAZONO Mikio, SAISHO Daisuke, TSUTSUMI Nobuhiro, HIRAI Atsushi

    Plant and cell physiology   38   s38   1997年3月

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講演・口頭発表等

  • 野生オオムギと栽培オオムギにおける胚乳細胞壁の厚さに関する解析

    日本育種学第130回講演会  2016年 

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  • 「コシヒカリ」と「ヒヨクモチ」との交雑に由来するイネ組換え自殖系統群における出穂期および農業形質のQTL解析

    日本育種学第127回講演会  2015年 

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  • バイオリファイナリー利用に向けた稲わらの多様性

    日本育種学第127回講演会  2015年 

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  • オオムギ発芽時耐塩性のQTL解析

    日本育種学第127回講演会  2015年 

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  • 稲わらのバイオリファイナリー関連形質における遺伝解析

    日本育種学第128回講演会  2015年 

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  • Aluminum tolerance confers local adaptation into East Asia on domesticated barley.

    2014年 

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  • 栽培オオムギのアルミニウム耐性がもたらす東アジアへの適応分化.

    日本育種学第125回講演会  2014年 

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  • 「FieldBook」を使ったオオムギ遺伝資源の大規模表現型測定.

    日本育種学第124回講演会  2013年 

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  • Large scale phenotyping of barley germplasm using "FieldBook"

    2013年 

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  • オオムギのアルミニウム耐性獲得の進化過程

    日本育種学会第122回講演会  2012年 

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  • Evolutionary process of aluminum tolerance acquisition in barley

    2012年 

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  • Natural variation of vernalization requirements in domesticated barley: Higher grades of vernalization requirements are biasedly distributed in East Asia

    2011年 

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  • オオムギ種子の耐水性の地理的分布.

    日本育種学会第119回講演会  2011年 

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  • Implication of quantitative variation of vernalization requirement as an adaptive trait in barley

    SMBE  2011年 

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  • 栽培オオムギにおける春化要求性の自然変異:高度秋播性は東アジアに偏在する.

    日本育種学会第120回講演会  2011年 

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  • Geographic distribution of pre-germination flooding tolerance in barley

    2011年 

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  • 皮性・裸性を支配するNud遺伝子の多型からみた栽培オオムギの起源

    日本育種学会第117回講演会  2010年 

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  • 栽培オオムギにおける六条性の進化過程.

    日本育種学会第116回講演会  2009年 

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  • Evolutionary process of six-rowed spike in domesticated barley.

    6th International Triticeae Symposium.  2009年 

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  • 栽培オオムギβ-アミラーゼの熱安定性における多様性の成立過程.

    日本育種学会第114回講演会  2008年 

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  • ‘東亜型オオムギ’の起源と成立

    2007年度 遺伝学研究所研究会「イネ発生研究の新展開」  2008年 

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  • ユーラシア大陸に分布する栽培オオムギの分子系統地理学的解析.

    日本育種学会第112回講演会  2007年 

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  • 分子系統地理学からみる栽培オオムギの起源と伝播

    理研PSC 作物・樹木勉強会  2007年 

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  • 分子系統地理学からみる栽培オオムギの起源と伝播

    第17回QTLゲノム育種研究センター セミナー  2007年 

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  • 旧世界における栽培オオムギの起源と伝播

    2007年度 国立遺伝学研究所 研究集会 「植物種内多様性研究の最前線:進化、生態、リソース、情報」  2007年 

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  • Classification of beta-Amylase Alleles in Cultivated Barley and Wild Relatives.

    Plant & Animal Genomes XIII  2005年 

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  • オオムギ遺伝資源からのβ-アミラーゼ新規アリルの探索.

    日本育種学会第107・108回講演会  2005年 

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  • Identification of barley semi-dwarf gene ‘uzu’.

    9th International Barley Genetics Symposium  2004年 

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  • オオムギ7H染色体裸性遺伝子座領域のイネとのシンテニー.

    日本育種学会第106回講演会.  2004年 

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  • 醸造用オオムギ「はるな二条」 BACライブラリーの構築と利用.

    日本育種学会第105回講演会  2004年 

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  • オオムギ半矮性遺伝子”渦”の同定

    2003年 ムギ類分子生物学研究会  2003年 

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  • CONSTRUCTION AND EVALUATION OF A BAC LIBRARY FROM JAPANESE MALTING BARLEY ‘HARUNA NIJO’

    Plant, Animal & Microbe Genomes XI  2003年 

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  • オオムギ半矮性遺伝子‘渦’はブラシノステロイド非感受性変異に起因する.

    日本植物生理学会2003年度年会および第43回シンポジウム  2003年 

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  • オオムギ半矮性遺伝子‘渦’の同定.

    日本育種学会第103回講演会  2003年 

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  • 渦遺伝子がオオムギ未熟胚由来カルスからの植物体再分化におよぼす影響

    日本育種学会第104回講演会  2003年 

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  • CONSTRUCTION OF A BAC LIBRARY FROM JAPANESE MALTING BARLEY HARUNA NIJO.

    Plant, Animal & Microbe Genomes X  2002年 

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  • オオムギ半矮性遺伝子uzu座に連鎖するマイクロサテライトマーカーの遺伝的多様性.

    日本育種学会講演会  2002年 

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  • 醸造用オオムギ”はるな二条”のBACライブラリー構築.

    日本育種学会講演会  2002年 

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  • オオムギにおける半矮性「渦」の特性解析

    日本育種学会講演会  2002年 

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  • A large scale barley cDNA sequencing program in Okayama University.

    Plant & Animal Genome IX  2001年 

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  • オオムギのcDNAシークエンス解析 : ビールオオムギ「はるな二条」と日本在来品種「赤神力」との比較

    日本育種学会第98回講演会  2000年 

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  • シロイヌナズナalternative oxidase遺伝子ファミリーのコピー特異的発現制御.

    日本育種学会第96回講演会  1999年 

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受賞

  • 日本育種学会論文賞

    2005年  

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    受賞国:日本国

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  • 植物多様性遺伝学 (2021年度) 前期  - 木5~8

  • 生物資源科学特別研究 (2021年度) 通年  - その他

  • 植物ゲノムダイナミックス (2020年度) 第2学期  - 月5,月6

  • 植物ゲノム多様性遺伝学 (2020年度) 特別  - その他

  • 植物多様性解析学演習 (2020年度) 前期  - その他

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  • 植物多様性解析学演習 (2020年度) 前期  - その他

  • 植物多様性遺伝学 (2020年度) 前期  - その他

  • 生物資源科学特別研究 (2020年度) 通年  - その他

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