研究者詳細 - 山路　公造

論文 - 山路　公造

分割表示 45 件中 1 - 20 件目／全件表示 >>

Effects of Low-intensity Pulsed Ultrasound on Bone Formation with Gelatin Hydrogel Containing Bone Morphogenetic Protein-2 査読

Yamaji Kozo. Yokoyama Akihito. Sugaya Tsutomu. Matsuzaki Kumiko. Takahashi Kei, Shinno Yasuo. Ohara Naoko. Suzuki Shigeru

J Oral Tissue Engin 22 ( 2 ) 53 - 59 2024年12月

Effects of BMP-2 on bone response using gelatin hydrogel in estrogen-deficient rats 査読

Yamaji Kozo, Yokoyama Akihoto, Matsuzaki Kumoko, Sugaya Tsutomu, Shimada Yasushi, Ohara Naoko, Yoshiyama Masahiro

J Oral Tissue Engin 20 ( 2 ) 41 - 46 2022年12月

Effect of gelatin hydrogel containing BMP-2 on bone formation in aged rats 査読

Yamaji Kozo, Yokoyama Akihito, Matsuzaki Kumoko, Ohara Naoko, Sugaya Tsutomu, Yoshiyama Masahiro

J Oral Tissue Engin 19 ( 1 ) 10 - 14 2021年9月

Assessment of Demineralization Inhibition Effects of Dentin Desensitizers Using Swept-Source Optical Coherence Tomography. 査読国際誌

Kumiko Matsuzaki, Yasushi Shimada, Yasuo Shinno, Serina Ono, Kozo Yamaji, Naoko Ohara, Alireza Sadr, Yasunori Sumi, Junji Tagami, Masahiro Yoshiyama

Materials (Basel, Switzerland) 14 ( 8 ) 2021年4月

記述言語：英語掲載種別：研究論文（学術雑誌）

The purpose of this study was to evaluate the mechanism of action and the inhibiting effects of two types of desensitizers against dentin demineralization using pre-demineralized hypersensitivity tooth model in vitro. In this study, we confirmed that a hypersensitivity tooth model from our preliminary experiment could be prepared by immersing dentin discs in an acetic acid-based solution with pH 5.0 for three days. Dentin discs with three days of demineralization were prepared and applied by one of the desensitizers containing calcium fluoro-alumino-silicate glass (Nanoseal, NS) or fluoro-zinc-silicate glass (Caredyne Shield, CS), followed by an additional three days of demineralization. Dentin discs for three days of demineralization (de3) and six days of demineralization (de6) without the desensitizers were also prepared. The dentin discs after the experimental protocol were scanned using swept-source optical coherence tomography (SS-OCT) to image the cross-sectional (2D) view of the samples and evaluate the SS-OCT signal. The signal intensity profiles of SS-OCT from the region of interest of 300, 500, and 700 µm in depth were obtained to calculate the integrated signal intensity and signal attenuation coefficient. The morphological differences and remaining chemical elements of the dentin discs were also analyzed using scanning electron microscopy and energy-dispersive X-ray spectroscopy. SS-OCT images of CS and NS groups showed no obvious differences between the groups. However, SS-OCT signal profiles for both the CS and NS groups showed smaller attenuation coefficients and larger integrated signal intensities than those of the de6 group. Reactional deposits of the desensitizers even after the additional three days of demineralization were observed on the dentin surface in NS group, whereas remnants containing Zn were detected within the dentinal tubules in CS group. Consequently, both CS and NS groups showed inhibition effects against the additional three days of demineralization in this study. Our findings demonstrate that SS-OCT signal analysis can be used to monitor the dentin demineralization and inhibition effects of desensitizers against dentin demineralization in vitro.

DOI： 10.3390/ma14081876

PubMed

researchmap

Effects of Direct Pulp Capping on Hard Tissue Formation by Using Alginate Gel Containing Bone Morphogenetic Protein-2 査読

Yokoyama Akihito, Yamaji Kozo, Ohara Naoko, Matsuzaki Kumiko, Shimada Yasushi, Yoshiyama Masahiro

J Oral Tissue Engin 17 ( 2 ) 53 - 58 2019年12月

Effects of Transforming growth factor-β1 and Bone morphogenetic protein-2 on bone formation using a bioabsorbable scaffold at palatal sites in rats 査読

Yamaji K, Yokoyama A, Shimada Y, Sugaya T, Ohara N, Matsuzaki K, Nishiyama E, Yoshiyama M

J Oral Tissue Engin 16 ( 2 ) 74 - 79 2018年12月

Effects of aging on bone formation induced by BMP-2 using low-intensity pulsed ultrasound 査読

Yamaji K, Yokoyama A, Matsuzaki K, Ohara N, Nishiyama E, Sugaya T, Yoshiyama M

Journal of Oral Tissue Engineering 14 ( 1 ) 65 - 69 2016年

Induction of ES cells from Human Bone Marrow Mesenchymal Stem Cells: Epigenetic Control and Initialization of the Cells 査読

Izawa S, Yamaji K, Hoshika T, Nishitani Y, Yoshiyama M

Journal of Oral Tissue Engineering 11 ( 3 ) 220 - 226 2014年

Effects of ultrasound on bone formation induced by BMP-2 査読

Yamaji K, Nishimura M, Nishitani Y, Izawa S, Hoshika T, Shiode S, Yoshiyama M

Journal of Oral Tissue Engineering 8 ( 1 ) 47 - 51 2010年

Effects of BMP-2 injected into the palatal sites on bone formation 査読

Yamaji K, Takahashi K, Nishitani Y, Izawa S, Horikawa G, Nishimura M, Hoshika T, Yoshiyama M

J Oral Tissue Engin 6 ( 1 ) 62 - 66 2008年

担当区分：筆頭著者記述言語：英語出版者・発行元：日本再生歯科医学会

This study was designed to evaluate the effects of recombinant human BMP-2 (rhBMP-2) injected into the palatal periosteal sites in Wister rats on bone formation. The implant sites were separately divided into four groups according to the dosage of rhBMP-2 : 0 μg, 0.1 μg, 0.5 μg and 1.0 μg. The rats were sacrificed at 3 weeks after implantation, followed by histopathologic observation and histometric evaluation of new bone. Implants had disappeared at every group and new bone formation was observed at 0.1 μg, 0.5 μg and 1.0 μg. New bone was continuous with the original bone at 0.5 μg and 1.0 μg. Thickness of new bone increased as the dosage increased from 0 μg to 0.5 μg (P<0.05), and did not significantly change as the dosage increased from 0.5 μg to 1.0 μg. These results suggested that rhBMP-2 injected into the palatal periosteal sites could lead to induce new bone formation, although its effects varied according to the dosage of rhBMP-2.

DOI： 10.11223/jarde.6.62

CiNii Article

CiNii Books

researchmap

Dentin Regeneration by Direct Pulp Capping using rhBMP-2 査読

YAMAJI Kozo, HORIKAWA Gen, NISHITANI Yoshihiro, IZAWA Shunji, YOSHIYAMA Masahiro

Journal of Oral Tissue Engineering 5 ( 3 ) 145 - 149 2008年

担当区分：筆頭著者記述言語：英語出版者・発行元：日本再生歯科医学会

The purpose of this study was to evaluate the effect of direct pulp capping using recombinant human BMP-2 (rhBMP-2) on dentin regeneration. RhBMP-2 (1.0 mg/ml) was dissolved in alginate-based gel (AG). Cavities with the exposed pulp area were prepared of first molars in Wister rats. All cavities were divided into two groups: The exposed pulps were covered with AG contained rhBMP-2 (BMP group), and covered with AG alone (AG group). Then they were sealed with glass ionomer cement (Fuji IILC EM). The animals were sacrificed at 3 and 8 weeks after operation. In both groups, the covered materials were observed at 3 weeks after operation, while they had disappeared and the tertiary dentin formation was observed into the prepared cavity at 8 weeks after operation. Thickness of the tertiary dentin of BMP group was significantly higher than that of AG group (P<0.05). These results suggest that AG is effective scaffold and direct pulp capping using AG contains rhBMP-2 is capable of enhancing dentin regeneration.

DOI： 10.11223/jarde.5.145

CiNii Article

researchmap

Effects of dose of recombinant human BMP-2 on bone formation at palatal sites in young and old rats. 査読

Kozo Yamaji, Masamitsu Kawanami, Atsushi Matsumoto, Tomoomi Odajima, Yoshihiro Nishitani, Kensuke Iwasaka, Keiko Yoshimitsu, Masahiro Yoshiyama

Dental materials journal 26 ( 4 ) 481 - 6 2007年7月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC DENTAL MATERIALS DEVICES

This study was designed to examine the effects of dose of recombinant human BMP-2 (rhBMP-2) on bone formation at palatal sites in 10-week-old (10w) and 70-week-old (70w) rats, when combined with a polylactate-polyglycolate copolymer/gelatine sponge (PGS). New bone formation was observed at six weeks after implantation. In the 10w rats, thickness of new bone (TNB) increased as the dosage increased from 0 microg to 4 microg, and decreased significantly as the dosage increased from 8 microg to 24 microg. In contrast, in the 70w rats, TNB increased as the dosage increased from 0 microg to 16 microg, and did not significantly change as the dosage increased from 16 microg to 24 microg. These results suggest that the most effective dosage of rhBMP-2 for induction of bone formation varies according to age.

DOI： 10.4012/dmj.26.481

Web of Science

PubMed

researchmap

骨粗鬆症がBMP-2の骨形成反応に及ぼす影響査読

土屋奈央子, 小田島朝臣, 山路公造, 川浪雅光

日本歯科保存学雑誌 50 ( 6 ) 792 - 798 2007年

記述言語：日本語出版者・発行元：特定非営利活動法人日本歯科保存学会

BMP-2の骨再生における高い有効性は広く知られているが,骨粗鬆症マウスでの報告はない.本研究の目的は,骨粗鬆症がBMP-2の骨形成反応に及ぼす影響を,病理組織学的観察および組織学的計測によって検討することである.実験には,5週齢(メス)のOCIF/Jcl(homo)KOマウス(骨粗鬆症群)とC57BL/6NJclマウス(正常群)を用い,BMP-2をおのおの0,1,5,10,15μg/mlに調整したアテロコラーゲンゲルを頭蓋骨膜下へ注入した.病理組織学的観察をし,新生骨量および骨密度の計測を行った.統計学的有意差検定にはMann-WhitneyのU検定を用いた.両群とも,濃度依存的に新生骨量が増加する傾向がみられた.群間で比較すると,BMP-2濃度が0および1μg/mlのときは,両群ともにほとんど新生骨を認めず,5および15μg/mlのときは,骨粗鬆症群のほうが有意に多かった.10μg/mlのときは,骨粗鬆症群のほうが多い傾向を示したが有意差はなかった.新生骨の骨密度は5,10,15μg/mlのとき,骨粗鬆症群のほうが有意に低かった.以上の結果より,骨粗鬆症群では,BMP-2による新生骨量は増加するものの,骨密度が低い骨が形成されることが示唆された.

DOI： 10.11471/shikahozon.50.792

CiNii Article

CiNii Books

researchmap

平成18年度岡山大学医学部・歯学部附属病院歯科医師臨床研修における歯科保存分野診療研修の分析査読

河野隆幸, 山路公造, 吉山昌宏, 新井英雄, 高柴正悟, 鳥井康弘

日本歯科保存学雑誌 50 ( 6 ) 731 - 739 2007年

記述言語：日本語出版者・発行元：特定非営利活動法人日本歯科保存学会

岡山大学医学部・歯学部附属病院卒後臨床研修センター歯科研修部門(研修センター)では,平成18年から,定員65名の研修歯科医を受け入れ,電子ポートフォリオシステムを用いた卒後研修を行っている.今回,研修歯科医の歯科保存分野研修状況を把握し,岡山大学医学部・歯学部附属病院での研修教育システムを充実することを目的として,平成18年度に研修センターにおいて1年間研修を行った研修歯科医42名を対象に,担当した患者数や歯科保存分野の診療研修の状況を,本院で使用している電子ポートフォリオシステムを用いて集計・分析を行った.研修歯科医が1年間の研修期間において担当する患者数の平均は,17.0±4.9人であった.また,電子ポートフォリオから抽出した処置項目の合計数は,23,911項目であった.これらの項目を,歯科保存,補綴,口腔外科・歯科麻酔,小児歯科,矯正歯科,およびその他の診療分野の6分野に分類したところ,歯科保存分野が11,316項目(47.3%)と最も多かった.さらに,歯科保存分野を保存修復,歯内療法,および歯周治療の3分野に分類したところ,全体に占める割合は,保存修復分野が2,239項目(9.4%),歯内療法分野が2,335項目(9.8%),および歯周治療分野が6,742項目(28.2%)で,歯周治療分野が最も多かった.そのなかで,根管充填とSRP(Scalingand Root Planing)の研修歯科医1人当たりの平均経験数は10回を超えており,レジン充填,根管拡大・貼薬,歯周組織検査,ブラッシング指導,超音波スケーリング,そして,SPT(Supportive Periodontal Therapy)の研修歯科医1人当たりの平均経験数は20回を超えていた.以上から,岡山大学医学部・歯学部附属病院における臨床研修は,歯科保存分野の研修が約半数を占めており,基本的な歯科保存分野の治療に関しては,10回以上経験している項目が多いことがわかった.

DOI： 10.11471/shikahozon.50.731

CiNii Article

CiNii Books

researchmap

Study on Bone Formation Induced by Recombinant Human BMP-2 at Palatal Sites in Adult Rats 査読

YAMAJI Kozo, ITOTA Toshiyuki, IZAWA Shunji, NISHITANI Yoshihiro, YOSHIYAMA Masahiro

Journal of Oral Tissue Engineering 4 ( 1 ) 37 - 42 2006年

担当区分：筆頭著者記述言語：英語出版者・発行元：日本再生歯科医学会

This study was designed to examine the effects of bone formation induced by recombinant human BMP-2 (rhBMP-2), which was implanted together with a poly-lactate-polyglycolate-copolymer/gelatine sponge (PGS) at palatal sites in 30-week- old male Wister rats. The rats were divided into four groups according to the dose of rhBMP-2: 0µg, 4µg, 8µg and 16µg. New bone formation was observed in every group, and new bone was continuous with the original bone at 6 weeks after implantation. Thickness of new bone (TNB) increased as the dose increased from 0µg to 8µ, reached the maximal level at 8µg. In contrast, TNB decreased significantly as the dose increased from 8µg to 16µg. The results of this study indicate that new bone formation varies according to the dose of rhBMP-2 at palatal sites in adult rats.

DOI： 10.11223/jarde.4.37

CiNii Article

researchmap

Dentin Regeneration by Direct Pulp Capping Using a Bioabsorbable Material 査読

ITOTA Toshiyuki, HAYASHI Hiroyuki, TASHIRO Yoko, TAKAGI Ryo, YAMAJI Kozo, SHINNO Yasuo, IZAWA Shunji, OMAE Masanori, YOSHIYAMA Masahiro, KOMASA Yutaka

Journal of Oral Tissue Engineering 4 ( 1 ) 17 - 24 2006年

記述言語：英語出版者・発行元：日本再生歯科医学会

The aim of this study was to investigate the effect of bioabsorbable material on the direct pulp capping to know whether this material has a function in dentin regeneration by bioabsorption. Class V cavities with the exposed pulp area were prepared on the buccal surface of first molars in young beagle dogs. All cavities were conditioned with a self-etching primer (Clearfil SE Primer). In the cavities of right side (experimental group), the exposed pulp was covered with a bioabsorbable sheet (Kurabio AG), and then the cavities were coated with an adhesive agent (Clearfil SE Bond) and resorted with a resin composite. The left side cavities (control group) were applied the adhesive agent without the bioabsorbable sheet and restored with a resin composite. On three and six months after surgery, the teeth were extracted, immersion-fixed and decalcified. Afterward, the teeth were frozen-sectioned, stained with hematoxylin and eosin and observed using a light microscopy. In the experimental group, a large amount of the tertiary dentin was formed into the prepared cavity. On the other hand, the tertiary dentin was almost not formed into the cavity in the control group. The significantly tertiary dentin formation was recognized into the prepared cavity when the exposed pulp area had been covered by a bioabsorbable material. This result suggested that the space making by a bioabsorbable material could produce the derivation of pulp tissue and then dentin regeneration into the pre-pared cavity.

DOI： 10.11223/jarde.4.17

CiNii Article

researchmap

Bone Formation Induced by Recombinant Human BMP-2 Combined with Bioabsorbable Material at Subperiosteal Sites 査読

YAMAJI Kozo, NISHITANI Yoshihiro, IZAWA Shunji, SHINNO Yasuo, OMAE Masanori, YOSHIYAMA Masahiro

Journal of Oral Tissue Engineering 4 ( 2 ) 101 - 105 2006年

担当区分：筆頭著者記述言語：英語出版者・発行元：日本再生歯科医学会

The purpose of this study was to evaluate the effect of bone formation induced by recombinant human BMP-2 (rhBMP-2) combined with bioabsorbable material Kurabio^®AG (AG) at palatal subperiosteal sites in Wistar rats. AG was freeze-dried alginated gel dressing. Experimental sites were divided into three groups according to the implans : BMP group (rhBMP-2 combined with AG), AG group (AG alone) and Control group. In every group, infected region or atypical cell proliferation was not observed, and new bone formation was observed at 6 weeks after implantation. Thickness of new bone (TNB) of BMP group was significantly higher than that of AG and Control groups. There was no significant difference in TNB between AG and Control groups. These results suggest that rhBMP-2 combined with AG have the ability to promote bone formation, although AG alone do not have any effect on bone formation.

DOI： 10.11223/jarde.4.101

CiNii Article

researchmap

リコンビナントヒト BMP-2 の濃度が高齢ラットの口蓋部骨形成に及ぼす影響査読

山路公造, 小田島朝臣, 松本敦至, 川浪雅光

日本歯科保存学雑誌 45 ( 4 ) 710 - 715 2002年8月

Effect of aging on bone formation induced by recombinant human bone morphogenetic protein-2 combined with fibrous collagen membranes at subperiosteal sites 査読

A Matsumoto, K Yamaji, M Kawanami, H Kato

JOURNAL OF PERIODONTAL RESEARCH 36 ( 3 ) 175 - 182 2001年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MUNKSGAARD INT PUBL LTD

This study was designed to examine the effect of aging on bone formation induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) combined with a fibrous collagen membrane (FCM). Implantation was done subperiosteally in bilateral palatal grooves in 34 male Wistar rats divided into three age groups: a 10-week-old group(10w group), a 30-week-old group(30w group) and a 70-week-old group (70w group). RhBMP-2-combined FCMs were implanted on the left palatal grooves as BMP-implanted sites (BMP site), while rhBMP-2 was not implanted on the right palatal grooves as control sites. The rats were sacrificed 6 weeks after implantation, and histometric evaluations were performed, New bone formation was observed in every site of each age group and the new bone was almost completely continuous with the original bone. The new bone volume (NBV) of the BMP site was significantly higher than that of the control site in each age group, The NBV of both the control and BMP sites were highest in the 10w group and lowest in the 70w group. The disparity of NBV between the control and BMP sites, which indicated the response to implanted BMP excluding the effect of skeletal growth and surgical stimulation. did not significantly differ among the age groups. These results indicate that rhBMP-2-combined FCM has the ability to induct new bone Formation continuous with original bone even in senescent rats. Furthermore, it appeared that, in the case of palatal subperiosteal implantation, the responsiveness to implanted BMP was independent of age, although the total volume of newly formed bone declined with aging.

Web of Science

researchmap

ラットの急速に進行する実験的歯周炎における骨吸収に対するメナテトレノン(ビタミンK_2)投与による影響査読

小西秀和, 川浪雅光, 坂上竜資, 山路公造, 王佳敏, 加藤熈

日本歯科保存学雑誌 44 ( 2 ) 255 - 264 2001年4月

　前のページ - 次のページ　

<山路 公造>

論文 - 山路 公造

<山路　公造>

論文 - 山路　公造