Faculty Profiles - DANSAKO Hiromichi

写真a

DANSAKO Hiromichi

Organization

Faculty of Medicine, Dentistry and Pharmaceutical Sciences Associate Professor

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Degree

Ph.D（Medicine） ( 2004.9 Okayama University )
学士（農学） ( 1993.3 佐賀大学 )

Research Interests

ミトコンドリア機能不全
自然免疫機構
細胞外小胞
C型肝炎ウイルス（HCV）
B型肝炎ウイルス（HBV）

Research Areas

Life Science / Virology
Life Science / Cell biology

Research History

岡山大学学術研究院医歯薬学域准教授

2024.4

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岡山大学学術研究院医歯薬学域准教授

2021.4 - 2024.3

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岡山大学大学院医歯薬学総合研究科准教授

2016.4 - 2021.3

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岡山大学大学院医歯薬学総合研究科助教

2007.4 - 2016.3

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岡山大学大学院医歯薬学総合研究科助手

2005.4 - 2007.3

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岡山大学大学院医歯学総合研究科助手

2004.4 - 2005.3

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Okayama University 医学部第二内科

1997.4 - 2004.3

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Professional Memberships

日本細胞外小胞学会

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日本癌学会

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日本ウイルス学会

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Committee Memberships

岡山大学医学部CBT会場責任者

2024.10 - 2024.11

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岡山大学動物審査・組換えＤＮＡ審査合同ワーキングメンバー

2024.9

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岡山大学医学部CBT委員会副委員長

2024.4

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岡山大学高等学校等との教育連携推進会議4号委員

2024.4

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公益財団法人医療系大学間共用試験実施評価機構医学系CBT機構派遣監督者

2024.4

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岡山大学生命系交流科目班委員

2024.2

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岡山大学医学部CBT学内ブラッシュアップ委員会委員

2023

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Common Achievement Tests Organization 医学系CBT問題管理委員会タイプA専門部会

2023

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岡山大学組換えDNA実験安全管理委員会組換えDNA実験安全主任者

2020.4

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岡山大学研究用病原体等安全管理委員会委員

2020.4

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Frontiers in Microbiology 査読委員

2019.7

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岡山大学安全衛生推進機構運営会議リスクアセスメントワーキンググループ委員

2015.4 - 2016.3

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岡山大学医学部基礎・社会医学系教育企画委員会委員

2014.4

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岡山大学医学部共同実験室運営委員会委員

2014.4 - 2024.3

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Papers

Somatic mutations in tumor-infiltrating lymphocytes impact on antitumor immunity. Reviewed International journal

Fumiaki Mukohara, Kazuma Iwata, Takamasa Ishino, Takashi Inozume, Joji Nagasaki, Youki Ueda, Ken Suzawa, Toshihide Ueno, Hideki Ikeda, Katsushige Kawase, Yuka Saeki, Shusuke Kawashima, Kazuo Yamashita, Yu Kawahara, Yasuhiro Nakamura, Akiko Honobe-Tabuchi, Hiroko Watanabe, Hiromichi Dansako, Tatsuyoshi Kawamura, Yutaka Suzuki, Hiroaki Honda, Hiroyuki Mano, Shinichi Toyooka, Masahito Kawazu, Yosuke Togashi

Proceedings of the National Academy of Sciences of the United States of America 121 ( 35 ) e2320189121 2024.8

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Immune checkpoint inhibitors (ICIs) exert clinical efficacy against various types of cancers by reinvigorating exhausted CD8+ T cells that can expand and directly attack cancer cells (cancer-specific T cells) among tumor-infiltrating lymphocytes (TILs). Although some reports have identified somatic mutations in TILs, their effect on antitumor immunity remains unclear. In this study, we successfully established 18 cancer-specific T cell clones, which have an exhaustion phenotype, from the TILs of four patients with melanoma. We conducted whole-genome sequencing for these T cell clones and identified various somatic mutations in them with high clonality. Among the somatic mutations, an SH2D2A loss-of-function frameshift mutation and TNFAIP3 deletion could activate T cell effector functions in vitro. Furthermore, we generated CD8+ T cell-specific Tnfaip3 knockout mice and showed that Tnfaip3 function loss in CD8+ T cell increased antitumor immunity, leading to remarkable response to PD-1 blockade in vivo. In addition, we analyzed bulk CD3+ T cells from TILs in additional 12 patients and identified an SH2D2A mutation in one patient through amplicon sequencing. These findings suggest that somatic mutations in TILs can affect antitumor immunity and suggest unique biomarkers and therapeutic targets.

DOI： 10.1073/pnas.2320189121

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Stem-like progenitor and terminally differentiated TFH-like CD4+ T cell exhaustion in the tumor microenvironment. Reviewed International journal

Wenhao Zhou, Shusuke Kawashima, Takamasa Ishino, Katsushige Kawase, Youki Ueda, Kazuo Yamashita, Tomofumi Watanabe, Masahito Kawazu, Hiromichi Dansako, Yutaka Suzuki, Hiroyoshi Nishikawa, Takashi Inozume, Joji Nagasaki, Yosuke Togashi

Cell reports 43 ( 2 ) 113797 - 113797 2024.2

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Immune checkpoint inhibitors exert clinical efficacy against various types of cancer through reinvigoration of exhausted CD8+ T cells that attack cancer cells directly in the tumor microenvironment (TME). Using single-cell sequencing and mouse models, we show that CXCL13, highly expressed in tumor-infiltrating exhausted CD8+ T cells, induces CD4+ follicular helper T (TFH) cell infiltration, contributing to anti-tumor immunity. Furthermore, a part of the TFH cells in the TME exhibits cytotoxicity and directly attacks major histocompatibility complex-II-expressing tumors. TFH-like cytotoxic CD4+ T cells have high LAG-3/BLIMP1 and low TCF1 expression without self-renewal ability, whereas non-cytotoxic TFH cells express low LAG-3/BLIMP1 and high TCF1 with self-renewal ability, closely resembling the relationship between terminally differentiated and stem-like progenitor exhaustion in CD8+ T cells, respectively. Our findings provide deep insights into TFH-like CD4+ T cell exhaustion with helper progenitor and cytotoxic differentiated functions, mediating anti-tumor immunity orchestrally with CD8+ T cells.

DOI： 10.1016/j.celrep.2024.113797

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Treg細胞の活性化CTLA-4非依存性免疫抑制によるCTLA-4遮断の不安定な抗腫瘍効果(Disturbed anti-tumor effect of CTLA-4 blockade by activated CTLA-4-independent immunosuppression of Treg cells)

渡部智文, 石野貴雅, 上田優輝, 長崎譲慈, 河田達志, 定平卓也, 岩田健宏, 片山聡, 枝村康平, 小林泰之, 團迫浩方, 荒木元朗, 冨樫庸介

西日本泌尿器科学会総会抄録集 75回 185 - 185 2023.11

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Treg細胞の活性化CTLA-4非依存性免疫抑制によるCTLA-4遮断の不安定な抗腫瘍効果(Disturbed anti-tumor effect of CTLA-4 blockade by activated CTLA-4-independent immunosuppression of Treg cells)

渡部智文, 石野貴雅, 上田優輝, 長崎譲慈, 河田達志, 定平卓也, 岩田健宏, 片山聡, 枝村康平, 小林泰之, 團迫浩方, 荒木元朗, 冨樫庸介

西日本泌尿器科学会総会抄録集 75回 185 - 185 2023.11

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Hepatitis C virus NS5B triggers an MDA5-mediated innate immune response by producing dsRNA without the replication of viral genomes. Reviewed International journal

Hiromichi Dansako, Masanori Ikeda, Yasuo Ariumi, Yosuke Togashi, Nobuyuki Kato

The FEBS journal 2023.10

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During the replication of viral genomes, RNA viruses produce double-stranded RNA (dsRNA), through the activity of their RNA-dependent RNA polymerases (RdRps) as viral replication intermediates. Recognition of viral dsRNA by host pattern recognition receptors - such as retinoic acid-induced gene-I (RIG-I)-like receptors and Toll-like receptor 3 - triggers the production of interferon (IFN)-β via the activation of IFN regulatory factor (IRF)-3. It has been proposed that, during the replication of viral genomes, each of RIG-I and melanoma differentiation-associated gene 5 (MDA5) form homodimers for the efficient activation of a downstream signalling pathway in host cells. We previously reported that, in the non-neoplastic human hepatocyte line PH5CH8, the RdRp NS5B derived from hepatitis C virus (HCV) could induce IFN-β expression by its RdRp activity without the actual replication of viral genomes. However, the exact mechanism by which HCV NS5B produced IFN-β remained unknown. In the present study, we first showed that NS5B derived from another Flaviviridae family member, GB virus B (GBV-B), also possessed the ability to induce IFN-β in PH5CH8 cells. Similarly, HCV NS5B, but not its G317V mutant, which lacks RdRp activity, induced the dimerization of MDA5 and subsequently the activation of IRF-3. Interestingly, immunofluorescence analysis showed that HCV NS5B produced dsRNA. Like HCV NS5B, GBV-B NS5B also triggered the production of dsRNA and subsequently the dimerization of MDA5. Taken together, our results show that HCV NS5B triggers an MDA5-mediated innate immune response by producing dsRNA without the replication of viral genomes in human hepatocytes.

DOI： 10.1111/febs.16980

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CTLA-4の単純な阻害はTreg細胞のCTLA-4以外の免疫抑制機構の活性化を引き起こす(Anti-tumor effects of CTLA-4 blockade are distrubed by activated CTLA-4-independent immunosuppression of Treg cells)

渡部智文, 石野貴雅, 上田優輝, 長崎譲慈, 丸山雄樹, 河田達志, 定平卓也, 岩田健宏, 片山聡, 西村慎吾, 枝村康平, 小林知子, 小林泰之, 團迫浩方, 荒木元朗, 冨樫庸介

日本癌学会総会記事 82回 1380 - 1380 2023.9

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CTLA-4の単純な阻害はTreg細胞のCTLA-4以外の免疫抑制機構の活性化を引き起こす(Anti-tumor effects of CTLA-4 blockade are distrubed by activated CTLA-4-independent immunosuppression of Treg cells)

渡部智文, 石野貴雅, 上田優輝, 長崎譲慈, 丸山雄樹, 河田達志, 定平卓也, 岩田健宏, 片山聡, 西村慎吾, 枝村康平, 小林知子, 小林泰之, 團迫浩方, 荒木元朗, 冨樫庸介

日本癌学会総会記事 82回 1380 - 1380 2023.9

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Low frequency of intracranial progression in advanced NSCLC patients treated with cancer immunotherapies. Reviewed International journal

Naoya Kemmotsu, Kiichiro Ninomiya, Kei Kunimasa, Takamasa Ishino, Joji Nagasaki, Yoshihiro Otani, Hiroyuki Michiue, Eiki Ichihara, Kadoaki Ohashi, Takako Inoue, Motohiro Tamiya, Kazuko Sakai, Youki Ueda, Hiromichi Dansako, Kazuto Nishio, Katsuyuki Kiura, Isao Date, Yosuke Togashi

International journal of cancer 2023.8

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Intracranial metastases are common in nonsmall-cell lung cancer (NSCLC) patients, whose prognosis is very poor. In addition, intracranial progression is common during systemic treatments due to the inability to penetrate central nervous system (CNS) barriers, whereas the intracranial effects of cancer immunotherapies remain unclear. We analyzed clinical data to evaluate the frequency of intracranial progression in advanced NSCLC patients treated with PD-1 blockade therapies compared with those treated without PD-1 blockade therapies, and found that the frequency of intracranial progression in advanced NSCLC patients treated with PD-1 blockade therapies was significantly lower than that in patients treated with cytotoxic chemotherapies. In murine models, intracranial rechallenged tumors after initial rejection by PD-1 blockade were suppressed. Accordingly, long-lived memory precursor effector T cells and antigen-specific T cells were increased by PD-1 blockade in intracranial lesions. However, intracranial rechallenged different tumors are not suppressed. Our results indicate that cancer immunotherapies can prevent intracranial progression, maintaining long-term effects intracranially as well as systemically. If intracranial recurrence occurs during the treatment with PD-1 blockade therapies, aggressive local therapies could be worthwhile.

DOI： 10.1002/ijc.34700

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Combination therapy with hydrogen peroxide and irradiation promotes an abscopal effect in mouse models. Reviewed International journal

Naoya Kemmotsu, Li Zhu, Joji Nagasaki, Yoshihiro Otani, Youki Ueda, Hiromichi Dansako, Yue Fang, Isao Date, Yosuke Togashi

Cancer science 114 ( 10 ) 3848 - 3856 2023.7

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Hydrogen peroxide (H2 O2 ) induces oxidative stress and cytotoxicity, and can be used for treating cancers in combination with radiotherapy. A product comprising H2 O2 and sodium hyaluronate has been developed as a radiosensitizer. However, the effects of H2 O2 on antitumor immunity remain unclear. To investigate the effects of H2 O2 , especially the abscopal effect when combined with radiotherapy (RT), we implanted murine tumor cells simultaneously in two locations in mouse models: the hind limb and back. H2 O2 mixed with sodium hyaluronate was injected intratumorally, followed by irradiation only at the hind limb lesion. No treatment was administered to the back lesion. The H2 O2 /RT combination significantly reduced tumor growth at the noninjected/nonirradiated site in the back lesion, whereas H2 O2 or RT individually did not reduce tumor growth. Flow cytometric analyses of the tumor-draining lymph nodes in the injected/irradiated areas showed that the number of dendritic cells increased significantly with maturation in the H2 O2 /RT combination group. In addition, analyses of tumor-infiltrating lymphocytes showed that the number of CD8+ (cluster of differentiation 8) T cells and the frequency of IFN-γ+ (interferon gamma) CD8+ T cells were higher in the noninjected/nonirradiated tumors in the H2 O2 /RT group compared to those in the other groups. PD-1 (programmed death receptor 1) blockade further increased the antitumor effect against noninjected/nonirradiated tumors in the H2 O2 /RT group. Intratumoral injection of H2 O2 combined with RT therefore induces an abscopal effect by activating antitumor immunity, which can be further enhanced by PD-1 blockade. These findings promote the development of H2 O2 /RT therapy combined with cancer immunotherapies, even for advanced cancers.

DOI： 10.1111/cas.15911

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Activated CTLA-4-independent immunosuppression of Treg cells disturbs CTLA-4 blockade-mediated antitumor immunity. Reviewed International journal

Tomofumi Watanabe, Takamasa Ishino, Youki Ueda, Joji Nagasaki, Takuya Sadahira, Hiromichi Dansako, Motoo Araki, Yosuke Togashi

Cancer science 114 ( 5 ) 1859 - 1870 2023.2

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Combination therapy with anti-cytotoxic T lymphocyte-associated protein 4 (CTLA-4) and anti-programmed death-1 (PD-1) monoclonal antibodies (mAbs) has dramatically improved the prognosis of patients with multiple types of cancer, including renal cell carcinoma (RCC). However, more than half of RCC patients fail to respond to this therapy. Regulatory T cells (Treg cells) are a subset of highly immunosuppressive CD4+ T cells that promote the immune escape of tumors by suppressing effector T cells in the tumor microenvironment (TME) through various mechanisms. CTLA-4 is constitutively expressed in Treg cells and is regarded as a key molecule for Treg cell-mediated immunosuppressive functions, suppressing antigen-presenting cells by binding to CD80/CD86. Reducing Treg cells in the TME with an anti-CTLA-4 mAb with antibody-dependent cellular cytotoxicity (ADCC) activity is considered an essential mechanism to achieve tumor regression. In contrast, we demonstrated that only CTLA-4 blockade without ADCC activity enhanced CD28 costimulatory signaling pathways in Treg cells and promoted Treg-cell proliferation in mouse models. CTLA-4 blockade also augmented CTLA-4-independent immunosuppressive functions, including cytokine production, leading to insufficient antitumor effects. Similar results were also observed in human peripheral blood lymphocytes and tumor-infiltrating lymphocytes from patients with RCC. Our findings highlight the importance of Treg-cell depletion to achieve tumor regression in response to CTLA-4 blockade therapies.

DOI： 10.1111/cas.15756

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Extracellular vesicles activate ATM‐Chk2 signaling pathway through the intercellular transfer of mitochondrial DNA in HBV‐infected human hepatocytes Reviewed International journal

Hiromichi Dansako, Youki Ueda, Shinya Satoh, Nobuyuki Kato

The FASEB Journal 35 ( 6 ) e21680 2021.6

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Hepatitis B virus (HBV) is a human hepatotropic pathogen causing hepatocellular carcinoma. We recently obtained HBV-susceptible immortalized human hepatocyte NKNT-3 by exogenously expressing NTCP and its derived cell clones, #28.3.8 and #28.3.25.13 exhibiting different levels of HBV susceptibility. In the present study, we showed that HBV infection activated the ATM-Chk2 signaling pathway in #28.3.25.13 cells but not in #28.3.8 cells. Both the cell culture supernatant and extracellular vesicles (EVs) derived from HBV-infected #28.3.25.13 cells also activated the ATM-Chk2 signaling pathway in naïve #28.3.25.13 cells. Interestingly, EVs derived from HBV-infected #28.3.25.13 cells included higher level of mitochondrial DNA (mtDNA) than those from HBV-infected #28.3.8 cells. Based on our results, we propose the novel model that EVs mediate the activation of ATM-Chk2 signaling pathway by the intercellular transfer of mtDNA in HBV-infected human hepatocyte.

DOI： 10.1096/fj.202002678r

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1096/fj.202002678R
Antiviral mechanism of preclinical antimalarial compounds possessing multiple antiviral activities. Reviewed International journal

Weilin Gu, Youki Ueda, Hiromichi Dansako, Shinya Satoh, Nobuyuki Kato

FASEB bioAdvances 3 ( 5 ) 356 - 373 2021.5

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We previously found that N-89 and its derivative, N-251, which are being developed as antimalarial compounds, showed multiple antiviral activities including hepatitis C virus (HCV). In this study, we focused on the most characterized anti-HCV activity of N-89(N-251) to clarify their antiviral mechanisms. We first prepared cells exhibiting resistance to N-89(N-251) than the parental cells by serial treatment of HCV-RNA-replicating parental cells with N-89(N-251). Then, we newly generated HCV-RNA-replicating cells with the replacement of HCV-RNAs derived from N-89(N-251)-resistant cells and parental cells. Using these cells, we examined the degree of inhibition of HCV-RNA replication by N-89(N-251) and found that the host and viral factors contributed almost equally to the resistance to N-89(N-251). To further examine the contribution of the host factors, we selected several candidate genes by cDNA microarray analysis and found that the upregulated expression of at least RAC2 and CKMT1B genes independently and differently contributed to the acquisition of an N-89(N-251)-resistant phenotype. For the viral factors, we selected several mutation candidates by the genetic comparative analysis of HCV-RNAs and showed that at least one M414I mutation in the HCV NS5B contributed to the resistance to N-89. Moreover, we demonstrated that the combination of host factors (RAC2 and/or CKMT1B) and a viral factor (M414I mutation) additively increased the resistance to N-89. In summary, we identified the host and viral factors contributing to the acquisition of N-89(N-251)-resistance in HCV-RNA replication. These findings will be useful for clarification of the antiviral mechanism of N-89(N-251).

DOI： 10.1096/fba.2020-00107

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Identification of ribavirin-responsive cis-elements for GPAM suppression in the GPAM genome. Reviewed International journal

Daichi Onomura, Shinya Satoh, Youki Ueda, Hiromichi Dansako, Nobuyuki Kato

Biochemical and biophysical research communications 533 ( 1 ) 148 - 154 2020.11

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Glycerol-3-phosphate acyltransferase, mitochondrial (GPAM) is a rate-limiting enzyme catalyzing triglyceride synthesis. Recently, we demonstrated that the anti-viral drug ribavirin (RBV) reduces GPAM expression by downregulating CCAAT/enhancer-binding protein α (C/EBPα). However, the precise mechanisms of GPAM suppression have remained unclear. Here, we found that RBV suppressed GPAM expression by downregulating not only C/EBPα, but also sterol regulatory element-binding protein-1c (SREBP-1c). We also found that cis-elements regulated by C/EBPα and SREBP-1c functioned as distal and proximal enhancers, respectively, to express hepatocyte- and adipocytes-specific GPAM variants. These results imply that RBV disrupts formation of the enhancer machineries on the GPAM genome by downregulating both transcription factors. Our findings may contribute to the development of treatments for fatty liver diseases caused by aberrant triglyceride synthesis.

DOI： 10.1016/j.bbrc.2020.08.112

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Study of multiple genetic variations caused by persistent hepatitis C virus replication in long-term cell culture. Reviewed International journal

Nobuyuki Kato, Youki Ueda, Hiroe Sejima, Weilin Gu, Shinya Satoh, Hiromichi Dansako, Masanori Ikeda, Kunitada Shimotohno

Archives of virology 165 ( 2 ) 331 - 343 2020.2

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The most characteristic feature of the hepatitis C virus (HCV) genome in patients with chronic hepatitis C is its remarkable variability and diversity. To better understand this feature, we performed genetic analysis of HCV replicons recovered from two human hepatoma HuH-7-derived cell lines after 1, 3, 5, 7, and 9 years in culture: The cell lines 50-1 and sO harbored HCV 1B-1 and O strain-derived HCV replicons established in 2002 and 2003, respectively. The results revealed that genetic variations in both replicons accumulated in a time-dependent manner at a constant rate despite the maintenance of moderate diversity (less than 1.8% difference) between the clones and that the mutation rate in the 50-1 and sO replicons was 2.5 and 2.9 × 10-3 base substitutions/site/year, respectively. We found that the genetic distance of both replicons increased from 7.9% to 10.5% after 9 years in culture. In addition, we observed that the guanine + cytosine (GC) content of both replicon RNAs increased in a time-dependent manner, as observed in our previous studies. Finally, we demonstrated that the high sensitivity of both replicons to direct-acting antivirals was maintained even after 9 years in culture. Our results suggest that long-term cultured HCV replicon-harboring cells are a useful model for understanding the variability and diversity of the HCV genome and the drug sensitivity of HCV in patients with chronic hepatitis C.

DOI： 10.1007/s00705-019-04461-0

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A new hepatoma cell line exhibiting high susceptibility to hepatitis B virus infection. Reviewed International journal

Youki Ueda, Weilin Gu, Hiromichi Dansako, Hironori Nishitsuji, Shinya Satoh, Kunitada Shimotohno, Nobuyuki Kato

Biochemical and biophysical research communications 515 ( 1 ) 156 - 162 2019.7

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Hepatitis B virus (HBV) infection, which increases the risk of cirrhosis and hepatocellular carcinoma and requires lifelong treatment, has become a major global health problem. However, host factors essential to the HBV life cycle are still unclear, and the development of new drugs is needed. Cells derived from the human hepatoma cell line HepG2 and engineered to overexpress sodium taurocholate cotransporting polypeptide (NTCP: a receptor for HBV), termed HepG2/NTCP cells, are widely used as the cell-based HBV infection and replication systems for HBV research. We recently found that human hepatoma cell line Li23-derived cells overexpressing NTCP (A8 cells subcloned from Li23 cells), whose gene expression profile was distinct from that of HepG2/NTCP cells, were also sensitive to HBV infection. However, the HBV susceptibility of A8 cells was around 1/100 that of HepG2/NTCP cells. Since we considered that plural cell assay systems will be needed for the objective evaluation of anti-HBV reagents, as we previously demonstrated in hepatitis C virus research, we here attempted to develop a new Li23 cell-derived assay system equivalent to that using HepG2/NTCP cells. By repeated subcloning of A8 cells, we successfully established a new cell line (A8.15.78.10) exhibiting high HBV susceptibility equal to that of HepG2/NTCP cells. Characterization of A8.15.78.10 cells revealed that the increase of HBV susceptibility was correlated with increases in the protein and glycosylation levels of NTCP, and with decreased expression of STING, a factor contributing to innate immunity. Finally, we performed a comparative evaluation of HBV entry inhibitors (cyclosporin A and rosiglitazone) by an HBV/secNL reporter assay using A8.15.78.10 cells or HepG2/NTCP cells. The results confirmed that cyclosporin A exhibited anti-HBV activity in both cell lines, as previously reported. However, we found that rosiglitazone did not show the anti-HBV activity in A8.15.78.10 cells, although it worked in HepG2/NTCP cells as previously reported. This suggested that the difference in anti-HBV activity between cyclosporin A and rosiglitazone was due to the different types of cells used for the assay. In conclusion, plural assay systems using different types of cells are required for the objective and impartial evaluation of anti-HBV reagents.

DOI： 10.1016/j.bbrc.2019.05.126

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Ribavirin-induced down-regulation of CCAAT/enhancer-binding protein α leads to suppression of lipogenesis. Reviewed

Satoh S, Onomura D, Ueda Y, Dansako H, Honda M, Kaneko S, Kato N

The Biochemical journal 476 ( 1 ) 137 - 149 2019.1

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Publishing type：Research paper (scientific journal) Publisher：Portland Press Ltd.

<title>Abstract</title>
Recently, we demonstrated that the anti-viral drug ribavirin (RBV) had the ability to suppress lipogenesis through down-regulation of retinoid X receptor α (RXRα) under the control of the intracellular GTP-level and AMP-activated protein kinase-related kinases, especially microtubule affinity regulating kinase 4 (MARK4). RXRα-overexpression attenuated but did not abolish lipogenesis suppression by RBV, implying that additional factor(s) were involved in this suppressive effect. In the present study, we found that the protein level, but not the mRNA level, of CCAAT/enhancer-binding protein α (C/EBPα) was down-regulated by RBV in hepatic cells. Treatment with proteasome inhibitor attenuated RBV-induced down-regulation of C/EBPα, suggesting that RBV promoted degradation of C/EBPα protein via the ubiquitin–proteasome pathway. Depletion of intracellular GTP through inosine monophosphate dehydrogenase inhibition by RBV led to down-regulation of C/EBPα. In contrast, down-regulation of C/EBPα by RBV was independent of RXRα and MARK4. Knockdown of C/EBPα reduced the intracellular neutral lipid levels and the expression of genes related to the triglyceride (TG) synthesis pathway, especially glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), which encodes the first rate-limiting TG enzyme. Overexpression of C/EBPα yielded the opposite results. We also observed that RBV decreased GPAM expression. Moreover, overexpression of GPAM attenuated RBV-induced reduction in the intracellular neutral lipid levels. These data suggest that down-regulation of C/EBPα by RBV leads to the reduction in GPAM expression, which contributes to the suppression of lipogenesis. Our findings about the mechanism of RBV action in lipogenesis suppression will provide new insights for therapy against the active lipogenesis involved in hepatic steatosis and hepatocellular carcinomas.

DOI： 10.1042/BCJ20180680

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High-level expression of STING restricts susceptibility to HBV by mediating type III IFN induction Reviewed

Dansako, H., Imai, H., Ueda, Y., Satoh, S., Shimotohno, K., Kato, N.

FASEB BioAdvances 1 ( 2 ) 1 - 14 2019

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DOI： 10.1096/fba.1022

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Multiple antiviral activities of the antimalarial and anti-hepatitis C drug candidates N-89 and N-251. Reviewed International journal

Youki Ueda, Weilin Gu, Hiromichi Dansako, Hye-Sook Kim, Sayaka Yoshizaki, Nobuaki Okumura, Tomohiro Ishikawa, Hironori Nishitsuji, Fumihiro Kato, Takayuki Hishiki, Shinya Satoh, Koji Ishii, Michiaki Masuda, Kunitada Shimotohno, Masanori Ikeda, Nobuyuki Kato

Biochemistry and biophysics reports 15 1 - 6 2018.9

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The chemically synthesized endoperoxide compound N-89 and its derivative N-251 were shown to have potent antimalarial activity. We previously demonstrated that N-89 and N-251 potently inhibited the RNA replication of hepatitis C virus (HCV), which belongs to the Flaviviridae family. Since antimalarial and anti-HCV mechanisms have not been clarified, we were interested whether N-89 and N-251 possessed the activity against viruses other than HCV. In this study, we examined the effects of N-89 and N-251 on other flaviviruses (dengue virus and Japanese encephalitis virus) and hepatitis viruses (hepatitis B virus and hepatitis E virus). Our findings revealed that N-89 and N-251 moderately inhibited the RNA replication of Japanese encephalitis virus and hepatitis E virus, although we could not detect those anti-dengue virus activities. We also observed that N-89 and N-251 moderately inhibited the replication of hepatitis B virus at the step after viral translation. These results suggest the possibility that N-89 and N-251 act on some common host factor(s) that are necessary for viral replications, rather than the possibility that N-89 and N-251 directly act on the viral proteins except for HCV. We describe a new type of antiviral reagents, N-89 and N-251, which are applicable to multiple different viruses.

DOI： 10.1016/j.bbrep.2018.05.007

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Daunorubicin, a topoisomerase II poison, suppresses viral production of hepatitis B virus by inducing cGAS-dependent innate immune response. Reviewed International journal

Imai H, Dansako H, Ueda Y, Satoh S, Kato N

Biochemical and biophysical research communications 504 ( 4 ) 672 - 678 2018.9

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Hepatitis B virus (HBV) causes hepatic diseases such as chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. These diseases are closely associated with persistent HBV infection. To prevent the progression of hepatic diseases, it is thus important to suppress persistent HBV infection. Daunorubicin (DNR), a topoisomerase II (Top II) poison, is a clinically used anticancer agent with a wide spectrum of activity against malignancies. DNR was recently reported to cause DNA damage-dependent interferon (IFN)-β induction through exogenous cyclic GMP-AMP synthetase (cGAS) and subsequently to suppress Ebola virus replication. In the present study, we demonstrated that DNR caused the inhibition of cell proliferation, but not cell death, through the DNA damage response in immortalized human hepatocyte NKNT-3/NTCP cells. Interestingly, DNR triggered the endogenous cGAS-dependent innate immune response and subsequently suppressed viral production of HBV in NKNT-3/NTCP cells. Top II poisons may be anti-HBV drug candidates.

DOI： 10.1016/j.bbrc.2018.08.195

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ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell-mediated innate immune response in human hepatocytes Reviewed

Hiromichi Dansako, Hirotaka Imai, Youki Ueda, Shinya Satoh, Takaji Wakita, Nobuyuki Kato

FEBS Open Bio 8 ( 3 ) 361 - 371 2018.3

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DOI： 10.1002/2211-5463.12373

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Ribavirin suppresses hepatic lipogenesis through inosine monophosphate dehydrogenase inhibition: Involvement of adenosine monophosphate-activated protein kinase-related kinases and retinoid X receptor α. Reviewed

Satoh S, Mori K, Onomura D, Ueda Y, Dansako H, Honda M, Kaneko S, Ikeda M, Kato N

Hepatology communications 1 ( 6 ) 550 - 563 2017.8

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DOI： 10.1002/hep4.1065

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Evaluation of preclinical antimalarial drugs, which can overcome direct acting antivirals-resistant hepatitis C viruses, using the viral reporter assay systems Reviewed

Youki Ueda, Hiromichi Dansako, Shinya Satoh, Hye-Sook Kim, Yusuke Wataya, Hiroyuki Doi, Masanori Ikeda, Nobuyuki Kato

VIRUS RESEARCH 235 37 - 48 2017.5

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DOI： 10.1016/j.virusres.2017.03.015

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Rab13 Is Involved in the Entry Step of Hepatitis C Virus Infection Reviewed

Midori Takeda, Masanori Ikeda, Shinya Satoh, Hiromichi Dansako, Takaji Wakita, Nobuyuki Kato

ACTA MEDICA OKAYAMA 70 ( 2 ) 111 - 118 2016.4

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DOI： 10.18926/AMO/54190

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Molecular Mechanism Underlying the Suppression of CPB2 Expression Caused by Persistent Hepatitis C Virus RNA Replication Reviewed

Hiroe Sejima, Shinya Satoh, Hiromichi Dansako, Masao Honda, Shuichi Kaneko, Masanori Ikeda, Nobuyuki Kato

ACTA MEDICA OKAYAMA 70 ( 2 ) 75 - 88 2016.4

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DOI： 10.18926/AMO/54186

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The cyclic GMP-AMP synthetase-STING signaling pathway is required for both the innate immune response against HBV and the suppression of HBV assembly Reviewed

Hiromichi Dansako, Youki Ueda, Nobuaki Okumura, Shinya Satoh, Masaya Sugiyama, Masashi Mizokami, Masanori Ikeda, Nobuyuki Kato

FEBS JOURNAL 283 ( 1 ) 144 - 156 2016.1

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DOI： 10.1111/febs.13563

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Annexin A1 Negatively Regulates Viral RNA Replication of Hepatitis C Virus Reviewed

Hiroki Hiramoto, Hiromichi Dansako, Midori Takeda, Shinya Satoh, Takaji Wakita, Masanori Ikeda, Nobuyuki Kato

ACTA MEDICA OKAYAMA 69 ( 2 ) 71 - 78 2015.4

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DOI： 10.18926/AMO/53335

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Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells Reviewed

Nobuaki Okumura, Masanori Ikeda, Shinya Satoh, Hiromichi Dansako, Masaya Sugiyama, Masashi Mizokami, Nobuyuki Kato

FEBS LETTERS 589 ( 10 ) 1112 - 1118 2015.4

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DOI： 10.1016/j.febslet.2015.03.022

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[Recognition mechanism of hepatitis C viral infection]. Reviewed

Dansako H, Kato N

Nihon rinsho. Japanese journal of clinical medicine 73 ( 2 ) 229 - 233 2015.2

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Establishment of Hepatitis C Virus RNA-Replicating Cell Lines Possessing Ribavirin-Resistant Phenotype Reviewed

Shinya Satoh, Kyoko Mori, Youki Ueda, Hiroe Sejima, Hiromichi Dansako, Masanori Ikeda, Nobuyuki Kato

PLOS ONE 10 ( 2 ) e0118313 2015.2

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DOI： 10.1371/journal.pone.0118313

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Rab18 is required for viral assembly of hepatitis C virus through trafficking of the core protein to lipid droplets Reviewed

Hiromichi Dansako, Hiroki Hiramoto, Masanori Ikeda, Takaji Wakita, Nobuyuki Kato

VIROLOGY 462 166 - 174 2014.8

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DOI： 10.1016/j.virol.2014.05.017

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Anti-HCV activity of the Chinese medicinal fungus Cordyceps militaris Reviewed

Youki Ueda, Kyoko Mori, Shinya Satoh, Hiromichi Dansako, Masanori Ikeda, Nobuyuki Kato

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 447 ( 2 ) 341 - 345 2014.5

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DOI： 10.1016/j.bbrc.2014.03.150

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Genetic Characterization of Hepatitis C Virus in Long-Term RNA Replication Using Li23 Cell Culture Systems Reviewed

Nobuyuki Kato, Hiroe Sejima, Youki Ueda, Kyoko Mori, Shinya Satoh, Hiromichi Dansako, Masanori Ikeda

PLOS ONE 9 ( 3 ) e91156 2014.3

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DOI： 10.1371/journal.pone.0091156

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Co-immunoprecipitation of Flag-TLR3 or Myc-MSR1 with HCV RNA. Reviewed

Yamane D, Dansako H, Lemon SM

Bio-protocol 4 ( 4 ) 2014.2

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New Preclinical Antimalarial Drugs Potently Inhibit Hepatitis C Virus Genotype 1b RNA Replication Reviewed

Youki Ueda, Midori Takeda, Kyoko Mori, Hiromichi Dansako, Takaji Wakita, Hye-Sook Kim, Akira Sato, Yusuke Wataya, Masanori Ikeda, Nobuyuki Kato

PLOS ONE 8 ( 8 ) 2013.8

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DOI： 10.1371/journal.pone.0072519

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New Preclinical Antimalarial Drugs Potently Inhibit Hepatitis C Virus Genotype 1b RNA Replication Reviewed

Youki Ueda, Midori Takeda, Kyoko Mori, Hiromichi Dansako, Takaji Wakita, Hye-Sook Kim, Akira Sato, Yusuke Wataya, Masanori Ikeda, Nobuyuki Kato

PLOS ONE 8 ( 8 ) e72519 2013.8

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Class A Scavenger Receptor 1 (MSR1) Restricts Hepatitis C Virus Replication by Mediating Toll-like Receptor 3 Recognition of Viral RNAs Produced in Neighboring Cells Reviewed

Hiromichi Dansako, Daisuke Yamane, Christoph Welsch, David R. McGivern, Fengyu Hu, Nobuyuki Kato, Stanley M. Lemon

PLoS Pathogens 9 ( 5 ) e1003345 2013.5

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DOI： 10.1371/journal.ppat.1003345

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骨粗鬆症治療剤であるラロキシフェンの抗HCV活性機構について Reviewed

武田緑, 池田正徳, 森京子, 矢野雅彦, 有海康雄, 團迫浩方, 脇田隆字, 加藤宣之

肝臓 54 ( Suppl.1 ) A340 - A340 2013.4

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PML tumor suppressor protein is required for HCV production Reviewed

Misao Kuroki, Yasuo Ariumi, Makoto Hijikata, Masanori Ikeda, Hiromichi Dansako, Takaji Wakita, Kunitada Shimotohno, Nobuyuki Kato

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 430 ( 2 ) 592 - 597 2013.1

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DOI： 10.1016/j.bbrc.2012.11.108

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Development of a drug assay system with hepatitis C virus genome derived from a patient with acute hepatitis C Reviewed

Kyoko Mori, Youki Ueda, Yasuo Ariumi, Hiromichi Dansako, Masanori Ikeda, Nobuyuki Kato

VIRUS GENES 44 ( 3 ) 374 - 381 2012.6

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DOI： 10.1007/s11262-012-0712-2

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Raloxifene inhibits hepatitis C virus infection and replication Reviewed

Midori Takeda, Masanori Ikeda, Kyoko Mori, Masahiko Yano, Yasuo Ariumi, Hiromichi Dansako, Takaji Wakita, Nobuyuki Kato

FEBS OPEN BIO 2 279 - 283 2012

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DOI： 10.1016/j.fob.2012.08.003

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Anti-ulcer agent teprenone inhibits hepatitis C virus replication: potential treatment for hepatitis C Reviewed

Masanori Ikeda, Yoshinari Kawai, Kyoko Mori, Masahiko Yano, Ken-ichi Abe, Go Nishimura, Hiromichi Dansako, Yasuo Ariumi, Takaji Wakita, Kazuhide Yamamoto, Nobuyuki Kato

LIVER INTERNATIONAL 31 ( 6 ) 873 - 882 2011.7

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DOI： 10.1111/j.1478-3231.2011.02499.x

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Mechanism of action of ribavirin in a novel hepatitis C virus replication cell system Reviewed

Kyoko Mori, Masanori Ikeda, Yasuo Ariumi, Hiromichi Dansako, Takaji Wakita, Nobuyuki Kato

VIRUS RESEARCH 157 ( 1 ) 61 - 70 2011.4

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DOI： 10.1016/j.virusres.2011.02.005

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The ESCRT System Is Required for Hepatitis C Virus Production Reviewed

Yasuo Ariumi, Misao Kuroki, Masatoshi Maki, Masanori Ikeda, Hiromichi Dansako, Takaji Wakita, Nobuyuki Kato

PLOS ONE 6 ( 1 ) e14517 2011.1

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DOI： 10.1371/journal.pone.0014517

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Amino acid substitutions of hepatitis C virus core protein are not associated with intracellular antiviral response to interferon-alpha in vitro Reviewed

Fusao Ikeda, Hiromichi Dansako, Go Nishimura, Kyoko Mori, Yoshinari Kawai, Yasuo Ariumi, Yasuhiro Miyake, Akinobu Takaki, Kazuhiro Nouso, Yoshiaki Iwasaki, Masanori Ikeda, Nobuyuki Kato, Kazuhide Yamamoto

LIVER INTERNATIONAL 30 ( 9 ) 1324 - 1331 2010.10

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DOI： 10.1111/j.1478-3231.2010.02299.x

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C型肝炎ウイルスに対する新しい抗ウイルス剤スクリーニング系の開発 Reviewed

池田正徳, 森京子, 中澤貴秀, 有海康雄, 団迫浩方, 加藤宣之

肝臓 51 ( Suppl.2 ) A586 - A586 2010.9

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ヒト肝癌細胞株Li23由来の新しいHCV-RNA複製システムを用いたリバビリンの作用機序の解明 Reviewed

森京子, 池田正徳, 有海康雄, 團迫浩方, 加藤宣之

肝臓 51 ( Suppl.2 ) A586 - A586 2010.9

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新しいHCV-RNA複製系を用いたリバビリンの抗HCVメカニズムの解明(Mechanism of anti-HCV of ribavirin in a novel HCV replication cell system) Reviewed

森京子, 池田正徳, 有海康雄, 団迫浩方, 加藤宣之

日本癌学会総会記事 69回 91 - 91 2010.8

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がん抑制因子PMLはHCV生活環に必須である(The PML tumor suppressor protein is required for HCV life cycle) Reviewed

黒木美沙緒, 有海康雄, 池田正徳, 團迫浩方, 脇田隆字, 加藤宣之

日本癌学会総会記事 69回 91 - 91 2010.8

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DNA損傷センサーATMキナーゼとChk2はC型肝炎ウイルスのRNA複製に必要である Reviewed

有海康雄, 黒木美沙緒, 團迫浩方, 阿部健一, 池田正徳, 脇田隆字, 加藤宣之

岡山医学会雑誌 122 ( 1 ) 9 - 16 2010.4

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Efficient replication systems for hepatitis C virus using a new human hepatoma cell line Reviewed

Nobuyuki Kato, Kyoko Mori, Ken-ichi Abe, Hiromichi Dansako, Misao Kuroki, Yasuo Ariumi, Takaji Wakita, Masanori Ikeda

VIRUS RESEARCH 146 ( 1-2 ) 41 - 50 2009.12

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DOI： 10.1016/j.virusres.2009.08.006

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HCV genotype 1b chimeric replicon with NS5B of JFH-1 exhibited resistance to cyclosporine A Reviewed

Ken-ichi Abe, Masanori Ikeda, Yasuo Ariumi, Hiromichi Dansako, Takaji Wakita, Nobuyuki Kato

ARCHIVES OF VIROLOGY 154 ( 10 ) 1671 - 1677 2009.10

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DOI： 10.1007/s00705-009-0502-x

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HCVコア蛋白質のアミノ酸の違いとIFN応答性との関係についての培養細胞を用いた解析 Reviewed

池田房雄, 団迫浩方, 西村剛, 河合良成, 有海康雄, 池田正徳, 高木章乃夫, 岩崎良章, 加藤宣之, 山本和秀

肝臓 50 ( Suppl.2 ) A515 - A515 2009.9

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Development of a hepatitis C virus relapse model using genome-length hepatitis C virus ribonucleic acid-harboring cells possessing the interferon-alpha-resistance phenotype Reviewed

Yoshinari Kawai, Masanori Ikeda, Ken-ichi Abe, Masahiko Yano, Yasuo Ariumi, Hiromichi Dansako, Kazuhide Yamamoto, Nobuyuki Kato

HEPATOLOGY RESEARCH 39 ( 9 ) 898 - 909 2009.9

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DOI： 10.1111/j.1872-034X.2009.00525.x

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Oxidative Stress Induces Anti-Hepatitis C Virus Status via the Activation of Extracellular Signal-Regulated Kinase Reviewed

Masahiko Yano, Masanori Ikeda, Ken-ichi Abe, Yoshinari Kawai, Misao Kuroki, Kyoko Mori, Hiromichi Dansako, Yasuo Ariumi, Shougo Ohkoshi, Yutaka Aoyagi, Nobuyuki Kato

HEPATOLOGY 50 ( 3 ) 678 - 688 2009.9

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DOI： 10.1002/hep.23026

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Double-stranded RNA-induced interferon-beta and inflammatory cytokine production modulated by hepatitis C virus serine proteases derived from patients with hepatic diseases Reviewed

Hiromichi Dansako, Masanori Ikeda, Yasuo Ariumi, Takaji Wakita, Nobuyuki Kato

ARCHIVES OF VIROLOGY 154 ( 5 ) 801 - 810 2009.5

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DOI： 10.1007/s00705-009-0375-z

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Oncostatin M synergistically inhibits HCV RNA replication in combination with interferon-alpha Reviewed

Masanori Ikeda, Kyoko Mori, Yasuo Ariumi, Hiromichi Dansako, Nobuyuki Kato

FEBS LETTERS 583 ( 9 ) 1434 - 1438 2009.5

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DOI： 10.1016/j.febslet.2009.03.054

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Replicons from genotype 1b HCV-positive sera exhibit diverse sensitivities to anti-HCV reagents Reviewed

Go Nishimura, Masanori Ikeda, Kyoko Mori, Takahide Nakazawa, Yasuo Ariumi, Hiromichi Darisako, Nobuyuki Kato

ANTIVIRAL RESEARCH 82 ( 1 ) 42 - 50 2009.4

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DOI： 10.1016/j.antiviral.2009.01.007

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Arsenic Trioxide Inhibits Hepatitis C Virus RNA Replication through Modulation of the Glutathione Redox System and Oxidative Stress Reviewed

Misao Kuroki, Yasuo Ariumi, Masanori Ikeda, Hiromichi Dansako, Takaji Wakita, Nobuyuki Kato

JOURNAL OF VIROLOGY 83 ( 5 ) 2338 - 2348 2009.3

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DOI： 10.1128/JVI.01840-08

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Genetic variability and diversity of intracellular genome-length hepatitis C virus RNA in long-term cell culture Reviewed

Nobuyuki Kato, K. Abe, K. Mori, Y. Ariumi, H. Dansako, M. Ikeda

ARCHIVES OF VIROLOGY 154 ( 1 ) 77 - 85 2009.1

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DOI： 10.1007/s00705-008-0282-8

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The DNA damage sensors ataxia-telangiectasia mutated kinase and checkpoint kinase 2 are required for hepatitis C virus RNA replication Reviewed

Yasuo Ariumi, Misao Kuroki, Hiromichi Dansako, Ken-Ichi Abe, Masanori Ikeda, Takaji Wakita, Nobuyuki Kato

JOURNAL OF VIROLOGY 82 ( 19 ) 9639 - 9646 2008.10

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DOI： 10.1128/JVI.00351-08

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A new living cell-based assay system for monitoring genome-length hepatitis C virus RNA replication Reviewed

Hiromichi Dansako, Masanori Ikeda, Ken-ichi Abe, Kyoko Mori, Kazunori Takemoto, Yasuo Ariumi, Nobuyuki Kato

VIRUS RESEARCH 137 ( 1 ) 72 - 79 2008.10

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DOI： 10.1016/j.virusres.2008.06.001

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急性C型肝炎患者由来の新しいC型肝炎ウイルスゲノム複製系(New efficient replication system with hepatitis C virus genome derived from a patient with acute hepatitis C) Reviewed

森京子, 阿部健一, 團迫浩方, 有海康雄, 池田正徳, 加藤宣之

日本癌学会総会記事 67回 397 - 397 2008.9

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異なるHCV(1b型)株由来の全長HCV RNA複製レポーターアッセイ系の開発(Development of genome-length HCV RNA replication reporter assay systems using various genotype 1b HCV strains) Reviewed

池田正徳, 森京子, 阿部健一, 西村剛, 團迫浩方, 有海康雄, 中沢貴秀, 加藤宣之

日本癌学会総会記事 67回 482 - 482 2008.9

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ATM DNA損傷センサーはC型肝炎ウイルスのRNA複製に必要である(ATM, a DNA damage sensor, is required for hepatitis C virus RNA replication) Reviewed

有海康雄, 黒木美沙緒, 團迫浩方, 阿部健一, 池田正徳, 脇田隆字, 加藤宣之

日本癌学会総会記事 67回 482 - 483 2008.9

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New efficient replication system with hepatitis C virus genome derived from a patient with acute hepatitis C Reviewed

Kyoko Mori, Ken-ichi Abe, Hiromichi Dansako, Yasuo Ariumi, Masanori Ikeda, Nobuyuki Kato

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 371 ( 1 ) 104 - 109 2008.6

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DOI： 10.1016/j.bbrc.2008.04.005

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全長HCV-RNA複製細胞を基に作成したIFN治療後再発モデルによる有効な治療法の検討・評価 Reviewed

河合良成, 池田正徳, 阿部健一, 矢野雅彦, 有海康雄, 團迫浩方, 加藤宣之, 山本和秀

肝臓 49 ( Suppl.1 ) A172 - A172 2008.4

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抗HCV成分・製剤のHCV RNA複製に対する抑制機序におけるMAPK signaling pathwayの関与 Reviewed

矢野雅彦, 池田正徳, 阿部健一, 有海康雄, 團迫浩方, 大越章吾, 青柳豊, 加藤宣之

肝臓 49 ( Suppl.1 ) A136 - A136 2008.4

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DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication Reviewed

Yasuo Ariumi, Misao Kuroki, Ken-ichi Abe, Hiromichi Dansako, Masanori Ikeda, Takaji Wakita, Nobuyuki Kato

JOURNAL OF VIROLOGY 81 ( 24 ) 13922 - 13926 2007.12

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DOI： 10.1128/JVI.01517-07

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Limited suppression of the interferon-beta production by hepatitis C virus serine protease in cultured human hepatocytes Reviewed

Hiromichi Dansako, Masanori Ikeda, Nobuyuki Kato

FEBS JOURNAL 274 ( 16 ) 4161 - 4176 2007.8

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DOI： 10.1111/j.1742-4658.2007.05942.x

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DDX3 DEAD box RNAヘリカーゼはC型肝炎ウイルスのRNA複製に必要である(DDX3 DEAD box RNA helicase is required for hepatitis C virus RNA replication) Reviewed

有海康雄, 黒木美沙緒, 阿部健一, 團迫浩方, 池田正徳, 脇田隆字, 加藤宣之

日本癌学会総会記事 66回 237 - 237 2007.8

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全長HCV RNA複製細胞の長期培養におけるHCVの遺伝的変異動態(HCV genetic variability and dynamics in long-term culture of genome-length HCV RNA replicating cells) Reviewed

加藤宣之, 阿部健一, 森京子, 有海康雄, 團迫浩方, 池田正徳

日本癌学会総会記事 66回 291 - 292 2007.8

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Comprehensive analysis of the effects of ordinary nutrients on hepatitis C virus RNA replication in cell culture Reviewed

Masahiko Yano, Masanori Ikeda, Ken-ichi Abe, Hiromichi Dansako, Shogo Ohkoshi, Yutaka Aoyagi, Nobuyuki Kato

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 51 ( 6 ) 2016 - 2027 2007.6

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DOI： 10.1128/AAC.01426-06

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Serum-free cell culture system supplemented with lipid-rich albumin for hepatitis C virus (strain O of genotype 1b) replication Reviewed

Ken-ichi Abe, Masanori Ikeda, Yasuo Ariumi, Hiromichi Dansako, Nobuyuki Kato

VIRUS RESEARCH 125 ( 2 ) 162 - 168 2007.5

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DOI： 10.1016/j.virusres.2006.12.015

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Cell culture-adaptive NS3 mutations required for the robust replication of genome-length hepatitis C virus RNA Reviewed

Ken-ichi Abe, Masanori Ikeda, Hiromichi Dansako, Kazuhito Naka, Nobuyuki Kato

VIRUS RESEARCH 125 ( 1 ) 88 - 97 2007.4

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DOI： 10.1016/j.virusres.2006.12.011

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Tandem repeats of lactoferrin-derived anti-hepatitis C virus peptide enhance antiviral activity in cultured human hepatocytes Reviewed

Ken-ichi Abe, Akito Nozaki, Kazushi Tamura, Masanori Ikeda, Kazuhito Naka, Hiromichi Dansako, Hiro-o Hoshino, Katsuaki Tanaka, Nobuyuki Kato

MICROBIOLOGY AND IMMUNOLOGY 51 ( 1 ) 117 - 125 2007

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Different anti-HCV profiles of statins and their potential for combination therapy with interferon Reviewed

M Ikeda, K Abe, M Yamada, H Dansako, K Naka, N Kato

HEPATOLOGY 44 ( 1 ) 117 - 125 2006.7

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DOI： 10.1002/hep.21232

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Epigenetic silencing of interferon-inducible genes is implicated in interferon resistance of hepatitis C virus replicon-harboring cells Reviewed

K Naka, K Abe, K Takemoto, H Dansako, M Ikeda, K Shimotohno, N Kato

JOURNAL OF HEPATOLOGY 44 ( 5 ) 869 - 878 2006.5

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DOI： 10.1016/j.jhep.2006.01.030

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Hepatitis C virus NS5B delays cell cycle progression by inducing interferon-beta via Toll-like receptor 3 signaling pathway without replicating viral genomes Reviewed

K Naka, H Dansako, N Kobayashi, M Ikeda, N Kato

VIROLOGY 346 ( 2 ) 348 - 362 2006.3

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DOI： 10.1016/j.virol.2005.10.023

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Interferon resistance of hepatitis C virus replicon-harbouring cells is caused by functional disruption of type I interferon receptors Reviewed

K Naka, K Takemoto, K Abe, H Dansako, M Ikeda, K Shimotohno, N Kato

JOURNAL OF GENERAL VIROLOGY 86 ( Pt 10 ) 2787 - 2792 2005.10

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DOI： 10.1099/vir.0.81124-0

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Hepatitis C virus proteins exhibit conflicting effects on the interferon system in human hepatocyte cells Reviewed

H Dansako, K Naka, M Ikeda, N Kato

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 336 ( 2 ) 458 - 468 2005.10

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DOI： 10.1016/j.bbrc.2005.08.112

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Mizoribine inhibits hepatitis C virus RNA replication: Effect of combination with interferon-alpha Reviewed

K Naka, M Ikeda, KI Abe, H Dansako, N Kato

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 330 ( 3 ) 871 - 879 2005.5

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DOI： 10.1016/j.bbrc.2005.03.062

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Efficient replication of a full-length hepatitis C virus genome, strain O, in cell culture, and development of a luciferase reporter system Reviewed

M Ikeda, K Abe, H Dansako, T Nakamura, K Naka, N Kato

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 329 ( 4 ) 1350 - 1359 2005.4

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DOI： 10.1016/j.bbrc.2005.02.138

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Genetic variation and dynamics of hepatitis C virus replicons in long-term cell culture Reviewed

N Kato, T Nakamura, H Dansako, K Namba, K Abe, A Nozaki, K Naka, M Ikeda, K Shimotohno

JOURNAL OF GENERAL VIROLOGY 86 ( Pt 3 ) 645 - 656 2005.3

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DOI： 10.1099/vir.0.80479-0

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CDNA microarray analysis to compare HCV subgenomic replicon cells with their cured cells Reviewed

K Abe, M Ikeda, H Dansako, K Naka, K Shimotohno, N Kato

VIRUS RESEARCH 107 ( 1 ) 73 - 81 2005.1

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DOI： 10.1016/j.virusres.2004.06.013

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cDNA microarray analysis of lactoferrin expression in non-neoplastic human hepatocyte PH5CH8 cells. Reviewed

Tamura T, Nozaki A, Abe K, Dansako H, Naka K, Ikeda M, Tanaka K, Kato N

Biochimica et biophysica acta 1721 ( 1-3 ) 73 - 80 2005.1

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cDNA microarray analysis of lactoferrin expression in non-neoplastic human hepatocyte PH5CH8 cells Reviewed

T Tamura, A Nozaki, K Abe, H Dansako, K Naka, M Ikeda, K Tanaka, N Kato

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1721 ( 1-3 ) 73 - 80 2005.1

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DOI： 10.1016/j.bbagen.2004.10.003

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Establishment of hepatitis C virus replicon cell lines possessing interferon-resistant phenotype Reviewed

K Namba, K Naka, H Dansako, A Nozaki, M Ikeda, Y Shiratori, K Shimotohno, N Kato

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 323 ( 1 ) 299 - 309 2004.10

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DOI： 10.1016/j.bbrc.2004.08.091

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Promotion of microsatellite instability by hepatitis C virus core protein in human non-neoplastic hepatocyte cells Reviewed

A Naganuma, H Dansako, T Nakamura, A Nozaki, N Kato

CANCER RESEARCH 64 ( 4 ) 1307 - 1314 2004.2

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DOI： 10.1158/0008-5472.CAN-03-2992

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Differential activation of interferon-inducible genes by hepatitis C virus core protein mediated by the interferon stimulated response element Reviewed

H Dansako, A Naganuma, T Nakamura, F Ikeda, A Nozaki, N Kato

VIRUS RESEARCH 97 ( 1 ) 17 - 30 2003.11

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DOI： 10.1016/S0168-1702(03)00218-1

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Establishment of a hepatitis C virus subgenomic replicon derived from human hepatocytes infected in vitro Reviewed

N Kato, K Sugiyama, K Namba, H Dansako, T Nakamura, M Takami, K Naka, A Nozaki, K Shimotohno

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 306 ( 3 ) 756 - 766 2003.7

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DOI： 10.1016/S0006-291X(03)01047-7

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A novel fusion variant of the MORF and CBP genes detected in therapy-related myelodysplastic syndrome with t(10;16)(q22;p13) Reviewed

K Kojima, K Kaneda, C Yoshida, H Dansako, N Fujii, T Yano, K Shinagawa, M Yasukawa, S Fujita, M Tanimoto

BRITISH JOURNAL OF HAEMATOLOGY 120 ( 2 ) 271 - 273 2003.1

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Specificity of polymerase chain reaction-based clonality analysis of immunoglobulin heavy chain gene rearrangement for the detection of bone marrow infiltrate in B-cell lymphoma-associated haemophagocytic syndrome Reviewed

K Kojima, K Kaneda, M Yasukawa, K Tanaka, T Inoue, T Yamashita, H Dansako, ST Sakugawa, T Kozuka, M Hara, M Tanimoto

BRITISH JOURNAL OF HAEMATOLOGY 119 ( 3 ) 616 - 621 2002.12

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DOI： 10.1046/j.1365-2141.2002.03866.x

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Therapy-related myelodysplastic syndrome/acute myeloid leukemia M2 and translocation (8;21) Reviewed

M Sakugawa, K Kojima, K Kaneda, K Masuda, H Dansako, K Shinagawa, F Ishimaru, K Ikeda, K Niiya, M Harada, M Tanimoto

ANNALS OF HEMATOLOGY 80 ( 12 ) 763 - 766 2001.12

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Molecular characterization of the ERGIC-53 gene in two Japanese patients with combined factor V-factor VIII deficiency Reviewed

H. Dansako, F. Ishimaru, Y. Takai, J. Tomoda, K. Nakase, K. Fujii, Y. Ogama, T. Kozuka, N. Sezaki, K. Honda, M. Harada

Annals of Hematology 80 ( 5 ) 292 - 294 2001

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DOI： 10.1007/s002770000283

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Two novel gene mutations in type I antithrombin deficiency Reviewed

Kenji Niiya, Toru Kiguchi, Hiromichi Dansako, Kingo Fujimura, Takahiro Fujimoto, Kenji Iijima, Mitsune Tanimoto, Mine Harada

International Journal of Hematology 74 ( 4 ) 469 - 472 2001

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DOI： 10.1007/BF02982095

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Additional translocation (8;21)(q22;q22) in a patient with Philadelphia-positive chronic myelogenous leukaemia in the blastic phase Reviewed

K Kojima, M Yasukawa, F Ishimaru, H Dansako, Y Matsuo, Y Kimura, Y Nawa, M Hara, M Harada

BRITISH JOURNAL OF HAEMATOLOGY 106 ( 3 ) 720 - 722 1999.9

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Molecular characterization of total kininogen deficiency in Japanese patients Reviewed

Fumihiko Ishimaru, Hiromichi Dansako, Koichi Nakase, Nobuharu Fujii, Nobuo Sezaki, Hiroyuki Nakayama, Norihiko Fujii, Yutaka Komiyama, Kenji Iijima, Katsuto Takenaka, Takanori Teshima, Katsuji Shinagawa, Kazuma Ikeda, Kenji Niiya, Mine Harada

International Journal of Hematology 69 ( 2 ) 126 - 128 1999

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Books

生命科学のためのウイルス学 : 感染と宿主応答のしくみ, 医療への応用

Harper, D. R. (David R.), 下遠野, 邦忠, 瀬谷, 司（ Role： Joint translator , 第9章ウイルス、ベクターとゲノム学）

南江堂 2015.2 （ ISBN:9784524268375 ）

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日本臨牀 73巻 2号

（ Role： Contributor , C型肝炎ウイルス感染の認識機構）

日本臨牀社 2015

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臨牀消化器内科6月増刊号 C型肝炎のすべて

（ Role： Contributor , HCV持続感染のしくみ）

2014

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肝疾患Review 2006-2007

（ Role： Contributor , インターフェロンによるHCVの増殖制御）

2006

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Drug screening for suppression of neutral lipid synthesis by targeting C/EBPα

佐藤伸哉, 小野村大地, 渡士幸一, 上田優輝, 團迫浩方, 本多政夫, 金子周一, 鈴木哲朗, 加藤宣之

日本分子生物学会年会プログラム・要旨集(Web) 44th 2021

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クロファラビンは肝腫瘍ウイルスに対して多機能な抗ウイルス活性を示す

武田緑, 團迫浩方, 馬場昌範, 加藤宣之, 池田正徳

日本分子生物学会年会プログラム・要旨集(Web) 39th 2016

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C型肝炎ウイルスの阻害剤探索を目的としたスクリーニングシステムの開発

武田緑, 池田正徳, 佐藤伸哉, 團迫浩方, 加藤宣之

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集 88回・38回 [1P1149] - [1P1149] 2015.12

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【C型肝炎治療update】基礎研究の進歩 C型肝炎ウイルス感染の認識機構

團迫浩方, 加藤宣之

日本臨床 73 ( 2 ) 229 - 233 2015.2

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Other Link： https://search.jamas.or.jp/default/link?pub_year=2015&ichushi_jid=J01205&link_issn=&doc_id=20150128150008&doc_link_id=1522543654799529600&url=https%3A%2F%2Fcir.nii.ac.jp%2Fcrid%2F1522543654799529600&type=CiNii&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00003_1.gif
C型肝炎ウイルスの阻害剤探索を目的としたスクリーニングシステムの開発

武田緑, 武田緑, 池田正徳, 佐藤伸哉, 團迫浩方, 加藤宣之

日本生化学会大会(Web) 88th 2015

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小胞輸送蛋白質Rab13はHCV感染に必要な宿主因子である

武田緑, 池田正徳, 佐藤伸哉, 團迫浩方, 脇田隆字, 加藤宣之

肝臓 55 ( Suppl.2 ) A648 - A648 2014.9

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FOXAによるHBV複製制御機構の解明

奥村暢章, 池田正徳, 佐藤伸哉, 團迫浩方, 溝上雅史, 加藤宣之

肝臓 55 ( Suppl.2 ) A587 - A587 2014.9

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C型肝炎ウイルスRNAの長期細胞内複製によって発現低下した遺伝子の発現制御機構の解析(Analysis on regulation mechanism of genes whose expressions were reduced in long-term intracellular HCV-RNA replication)

瀬島寛恵, 佐藤伸哉, 團迫浩方, 池田正徳, 加藤宣之

日本癌学会総会記事 73回 P - 1037 2014.9

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臨床応用に向けた抗マラリア薬候補N-251の抗HCV活性作用機序の解析

上田優輝, 團迫浩方, 佐藤伸哉, 池田正徳, 加藤宣之

肝臓 55 ( Suppl.2 ) A647 - A647 2014.9

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HCV-RNAの長期複製によって発現低下したBASP1とCPB2遺伝子の発現制御機構の解析

瀬島寛恵, 佐藤伸哉, 團迫浩方, 池田正徳, 加藤宣之

肝臓 55 ( Suppl.2 ) A648 - A648 2014.9

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【C型肝炎のすべて】(第I章)HCV研究の最先端 HCV持続感染のしくみ

團迫浩方, 加藤宣之

臨床消化器内科 29 ( 7 ) 799 - 802 2014.5

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小胞輸送蛋白質Rab13はHCV感染に必要な宿主因子である

武田緑, 池田正徳, 佐藤伸哉, 團迫浩方, 脇田隆字, 加藤宣之

肝臓 55 ( Supplement 2 ) 2014

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HCVライフサイクルにおける小胞輸送蛋白質Rab13の役割

武田緑, 池田正徳, 佐藤伸哉, 團迫浩方, 脇田隆字, 加藤宣之

日本ウイルス学会学術集会プログラム・抄録集 62nd 2014

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C型肝炎ウイルスのライフサイクルにおけるRab13の重要性

武田緑, 武田緑, 池田正徳, 池田正徳, 佐藤伸哉, 團迫浩方, 脇田隆字, 加藤宣之

日本分子生物学会年会プログラム・要旨集(Web) 37th 2014

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2年間にわたるC型肝炎ウイルスRNAの細胞内複製によって発現量が変動したマイクロRNAの同定(Identification of microRNAs showing differential expression profiles in cell-based HCV RNA replication for 2 years)

瀬島寛恵, 森京子, 佐藤伸哉, 團迫浩方, 池田正徳, 加藤宣之

日本癌学会総会記事 72回 241 - 241 2013.10

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HBV複製系の開発に向けた肝細胞株の選択

奥村暢章, 池田正徳, 武田緑, 團迫浩方, 加藤宣之

肝臓 54 ( Suppl.2 ) A546 - A546 2013.9

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C型肝炎ウイルスの複製環境に関わるmicroRNAの探索

瀬島寛恵, 森京子, 團迫浩方, 池田正徳, 加藤宣之

肝臓 54 ( Suppl.1 ) A338 - A338 2013.4

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中国由来の漢方薬であるサナギタケ(北冬虫夏草)に見出された抗HCV活性

上田優輝, 森京子, 團迫浩方, 池田正徳, 加藤宣之

肝臓 54 ( Suppl.1 ) A341 - A341 2013.4

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HBV複製系の開発に向けた肝細胞株の選択

奥村暢章, 池田正徳, 武田緑, 團迫浩方, 加藤宣之

肝臓 54 ( Supplement 2 ) 2013

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ヒト肝細胞株におけるHBV複製能の評価

奥村暢章, 池田正徳, 武田緑, 佐藤伸哉, 團迫浩方, 溝上雅史, 加藤宣之

日本ウイルス学会学術集会プログラム・抄録集 61st 2013

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HBV持続感染培養系の確立に向けたヒト肝細胞株のHBV複製能の解析

奥村暢章, 池田正徳, 武田緑, 佐藤伸哉, 團迫浩方, 溝上雅史, 加藤宣之

日本分子生物学会年会プログラム・要旨集(Web) 36th 2013

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骨粗鬆症治療剤ラロキシフェンはC型肝炎ウイルスの増殖を抑制する

武田緑, 池田正徳, 森京子, 矢野雅彦, 有海康雄, 團迫浩方, 脇田隆字, 加藤宣之

日本分子生物学会年会プログラム・要旨集(Web) 36th 2013

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遺伝子型の異なるHCV培養細胞システムを用いたラロキシフェンの抗HCV活性の検討

武田緑, 池田正徳, 森京子, 矢野雅彦, 有海康雄, 團迫浩方, 脇田隆字, 加藤宣之

日本ウイルス学会学術集会プログラム・抄録集 61st 2013

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J-GLOBAL

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抗マラリア薬として開発中の化合物に見出された強力な抗HCV活性(Potent anti-HCV activities found in preclinical anti-malarial drugs)

上田優輝, 森京子, 団迫浩方, 金惠淑, 綿矢有佑, 池田正徳, 加藤宣之

日本癌学会総会記事 71回 116 - 116 2012.8

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抗HCV薬リバビリンに対する感受性を決定する宿主因子の多面的解析(Multifaceted analysis for a host factor determining the anti-HCV activity of ribavirin)

森京子, 本多政夫, 池田正徳, 団迫浩方, 金子周一, 加藤宣之

日本癌学会総会記事 71回 66 - 66 2012.8

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新しいHCV-RNA複製系を用いたリバビリンの抗HCVメカニズムの解明(Mechanism of anti-HCV of ribavirin in a novel HCV replication cell system)

森京子, 池田正徳, 有海康雄, 団迫浩方, 加藤宣之

日本癌学会総会記事 69回 91 - 91 2010.8

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がん抑制因子PMLはHCV生活環に必須である(The PML tumor suppressor protein is required for HCV life cycle)

黒木美沙緒, 有海康雄, 池田正徳, 團迫浩方, 脇田隆字, 加藤宣之

日本癌学会総会記事 69回 91 - 91 2010.8

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異なるHCV株,細胞株を用いたHCV RNA複製培養細胞での薬剤評価

池田正徳, 森京子, 森京子, 武田緑, 中澤貴秀, 有海康雄, 団迫浩方, 加藤宣之

日本ウイルス学会学術集会プログラム・抄録集 58th 2010

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OXIDATIVE STRESS INDUCES ANTI-HCV STATUS VIA THE ACTIVATION OF MEK-ERK1/2 SIGNALING PATHWAY

Masahiko Yano, Masanori Ikeda, Ken-ichi Abe, Yoshinari Kawai, Misao Kuroki, Kyoko Mori, Hiromichi Dansako, Yasuo Ariumi, Yasunobu Matsuda, Shougo Ohkoshi, Yutaka Aoyagi, Nobuyuki Kato

HEPATOLOGY 50 ( 4 ) 965A - 965A 2009.10

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Language：English Publishing type：Research paper, summary (international conference)

Web of Science

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C型肝炎ウイルスエンベロープ蛋白質に結合するラクトフェリン由来ペプチドの同定とその感染防御

阿部健一, 野崎昭人, 田村一志, 池田正徳, 仲一仁, 中村孝志, 團迫浩方, 星野洪郎, 田中克明, 加藤宣之

Milk Scinece 53:348-353 2004

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Dominant negative isoform of the Ikaros gene in patients with adult B-cell acute lymphoblastic leukemia

K Nakase, F Ishimaru, N Avitahl, H Dansako, K Matsuo, K Fujii, N Sezaki, H Nakayama, T Yano, S Fukuda, K Imajoh, M Takeuchi, A Miyata, M Hara, M Yasukawa, Takahashi, I, H Taguchi, K Matsue, S Nakao, Y Niho, K Takenaka, K Shinagawa, K Ikeda, K Niiya, M Harada

CANCER RESEARCH 60 ( 15 ) 4062 - 4065 2000.8

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B型肝炎ウイルスによるChk2キナーゼの活性化は細胞外小胞がミトコンドリアDNAを細胞間伝達することにより起こる

團迫浩方, 上田優輝, 佐藤伸哉, 加藤宣之

第７回日本細胞外小胞学会

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Event date： 2020.10.26 - 2020.10.27

Language：Japanese Presentation type：Oral presentation (general)

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A new hepatoma cell line exhibiting high susceptibility to HBV infection.

Ueda Y, Gu W, Dansako H, Nishitsuji H, Satoh S, Shimotohno K, Kato N

第67回日本ウイルス学会学術集会

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Event date： 2019.10.29 - 2019.10.31

Language：English Presentation type：Oral presentation (general)

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HBV-triggered antiviral signaling is transduced to neighboring cells by mediating extracellular vesicles in human immortalized hepatocyte NKNT-3

Dansako H, Imai H, Ueda Y, Satoh S, Kato N

第67回日本ウイルス学会学術集会

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Event date： 2019.10.29 - 2019.10.31

Language：English Presentation type：Oral presentation (general)

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細胞外小胞はB型肝炎ウイルス感染が誘導する自然免疫応答を細胞間で伝達する

團迫浩方, 今井大誉, 上田優輝, 佐藤伸哉, 加藤宣之

第6回日本細胞外小胞学会

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Event date： 2019.10.24 - 2019.10.25

Language：Japanese Presentation type：Oral presentation (general)

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A new human hepatoma cell line exhibiting high susceptibility to HBV infection. International conference

Youki Ueda,Hiromichi Dansako,Shinya Satoh,Kunitada Shimotohno,Nobuyuki Kato

2019 International HBV Meeting

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Event date： 2019.10.1 - 2019.10.5

Language：English Presentation type：Poster presentation

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ヒト不死化肝NKNT-3細胞において、細胞外小胞を介する細胞間自然免疫伝達機構の解明

團迫浩方, 今井大誉, 上田優輝, 佐藤伸哉, 加藤宣之

第34回中国四国ウイルス研究会

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Event date： 2019.6.29 - 2019.6.30

Language：Japanese Presentation type：Oral presentation (general)

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HBV高感受性新規肝がん細胞株の樹立

上田優輝, 谷?琳, 團迫浩方, 西辻裕紀, 佐藤伸哉, 下遠野邦忠, 加藤宣之

第34回中国四国ウイルス研究会

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Event date： 2019.6.29 - 2019.6.30

Language：Japanese Presentation type：Oral presentation (general)

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Highly expression of STING restricts HBV infection by mediating the induction of type III interferon in human immortalized hepatocyte NKNT-3

Dansako H, Imai H, Ueda Y, Satoh S, Shimotohno K, Kato N

第66回日本ウイルス学会学術集会

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Event date： 2018.10.28 - 2018.10.30

Language：English Presentation type：Oral presentation (general)

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Screening of the new host factors necessary for HBV proliferation using plural human cell lines

Ueda Y, Dansako H, Satoh S, Shimotohno K, Kato N

第66回日本ウイルス学会学術集会

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Event date： 2018.10.28 - 2018.10.30

Language：English Presentation type：Oral presentation (general)

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Mechanism of GPAM suppression by anti-HCV drug ribavirin

Onomura D, Satoh S, Ueda Y, Dansako H, Kato N

第66回日本ウイルス学会学術集会

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Event date： 2018.10.28 - 2018.10.30

Language：English Presentation type：Oral presentation (general)

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Analysis of host and viral factors contributing to resistant acquision for the preclinical anti-HCV drugs, N-89 and N-251

Gu W, Ueda Y, Satoh S, Dansako H, Kato N

第66回日本ウイルス学会学術集会

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Event date： 2018.10.28 - 2018.10.30

Language：English Presentation type：Oral presentation (general)

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Topoisomerase II inhibitor triggers cyclic GMP-AMP synthase-dependent innate immune response in human immortalized hepatocyte NKNT-3

Imai H, Dansako H, Ueda Y, Satoh S, Kato N

第66回日本ウイルス学会学術集会

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Event date： 2018.10.28 - 2018.10.30

Language：English Presentation type：Oral presentation (general)

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Drug screening for suppression of lipogenesis through downregulation of C/EBP-alpha

Satoh S, Onomura D, Watashi K, Ueda Y, Dansako H, Honda M, Kaneko S, Kato N

第66回日本ウイルス学会学術集会

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Event date： 2018.10.28 - 2018.10.30

Language：English Presentation type：Oral presentation (general)

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Screening of the new host factors necessary for HBV proliferation using plural human cell lines. International conference

Youki Ueda,Hiromichi Dansako,Shinya Satoh,Kunitada Shimotohno,Nobuyuki Kato

2018 International HBV Meeting

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Event date： 2018.10.3 - 2018.10.6

Language：English Presentation type：Poster presentation

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Analysis of proteins within exosomes secreted from hepatitis B virus-infected human immortalized hepatocyte NKNT-3

Dansako H, Imai H, Ueda Y, Satoh S, Kato N

第10回日本RNAi研究会・第5回日本細胞外小胞学会

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Event date： 2018.8.29 - 2018.8.31

Language：English Presentation type：Poster presentation

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抗HCV薬として開発中のN-89とN-251に対する抵抗性の獲得に寄与する宿主側とウイルス側因子の解析

谷?琳, 上田優輝, 佐藤伸哉, 團迫浩方, 加藤宣之

第33回中国四国ウイルス研究会

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Event date： 2018.6.23 - 2018.6.24

Language：Japanese Presentation type：Oral presentation (general)

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抗HCV薬リバビリンによる中性脂肪合成抑制機序の解明

佐藤伸哉, 小野村大地, 上田優輝, 團迫浩方, 加藤宣之

第33回中国四国ウイルス研究会

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Event date： 2018.6.23 - 2018.6.24

Language：Japanese Presentation type：Oral presentation (general)

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ヒト不死化肝NKNT-3細胞におけるtopoisomerase II阻害剤によるcGAS依存的な自然免疫応答の誘導

今井大誉, 團迫浩方, 上田優輝, 佐藤伸哉, 加藤宣之

第33回中国四国ウイルス研究会

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Event date： 2018.6.23 - 2018.6.24

Language：Japanese Presentation type：Oral presentation (general)

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HCV以外の広範なウイルスにも有効性を示した抗マラリア薬候補N-89とN-251

上田優輝, 谷?琳, 團迫浩方, 佐藤伸哉, 池田正徳, 加藤宣之

第33回中国四国ウイルス研究会

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Event date： 2018.6.23 - 2018.6.24

Language：Japanese Presentation type：Oral presentation (general)

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抗HCV薬リバビリンによるGPAM発現抑制機序の解明

小野村大地, 佐藤伸哉, 上田優輝, 團迫浩方, 加藤宣之

第33回中国四国ウイルス研究会

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Event date： 2018.6.23 - 2018.6.24

Language：Japanese Presentation type：Oral presentation (general)

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The cGas-STING signaling pathway is required for both the innate immune response against HBV and the suppression of HBV assembly

Hiromichi Dansako,Youki Ueda,Nobuaki Okumura,Shinya Satoh,Masaya Sugiyama,Masashi Mizokami,Masanori Ikeda,Nobuyuki Kato

第63回日本ウイルス学会学術集会

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Event date： 2015.11.22 - 2015.11.24

Language：English Presentation type：Oral presentation (general)

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cGAS-STINGシグナル経路はB型肝炎ウイルスの感染と会合を制御する

團迫浩方、上田優輝、奥村暢章、佐藤伸哉、杉山真也、溝上雅史、池田正徳、加藤宣之

第30回中国四国ウイルス研究会

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Event date： 2015.6.27 - 2015.6.28

Language：Japanese Presentation type：Oral presentation (general)

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B型肝炎ウイルスの細胞内複製の認識に重要なシグナル経路の解明

團迫浩方、佐藤伸哉、溝上雅史、池田正徳、加藤宣之

第62回日本ウイルス学会学術集会

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Event date： 2014.11.10 - 2014.11.12

Language：Japanese Presentation type：Oral presentation (general)

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Human Hepatoma HuH-7 Cell Line-derived RSc cells Show Higher Viral Productivity in Response to Infection with HCV-JFH-1 than Huh7.5 Cells International conference

Hiromichi Dansako,Hiroki Hiramoto,Masanori Ikeda,Takaji Wakita,Nobuyuki Kato

21st International Symposium on Hapatitis C and Related Viruses

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Event date： 2014.9.7 - 2014.9.11

Language：English Presentation type：Poster presentation

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B型肝炎ウイルスの細胞内複製を認識する宿主因子の探索

團迫浩方、佐藤伸哉、溝上雅史、池田正徳、加藤宣之

中国四国ウイルス研究会

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Event date： 2014.6.28 - 2014.6.29

Language：Japanese Presentation type：Oral presentation (general)

Venue：山口大学

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骨粗鬆症治療剤ラロキシフェンはC型肝炎ウイルスの増殖を抑制する

武田緑、池田正徳、森京子、矢野雅彦、有海康雄、團迫浩方、脇田隆字、加藤宣之

第36回日本分子生物学会

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Event date： 2013.12.3 - 2013.12.6

Language：Japanese Presentation type：Poster presentation

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HBV持続感染培養系の確立に向けたヒト肝細胞株のHBV複製能の解析

奥村暢章、池田正徳、武田緑、佐藤伸哉、團迫浩方、溝上雅史、加藤宣之

第36回日本分子生物学会

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Event date： 2013.12.3 - 2013.12.6

Language：Japanese Presentation type：Poster presentation

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抗HCV活性を有する中国由来の漢方薬であるサナギタケ（北冬虫夏草）

上田優輝、森京子、佐藤伸哉、團迫浩方、池田正徳、加藤宣之

第61回日本ウイルス学会学術集会

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Event date： 2013.11.10 - 2013.11.12

Language：Japanese Presentation type：Oral presentation (general)

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C型肝炎ウイルスのゲノム複製が２年間にわたることで発現レベルが変動したマイクロRNAの同定

瀬島寛恵、森京子、佐藤伸哉、團迫浩方、池田正徳、加藤宣之

第61回日本ウイルス学会学術集会

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Event date： 2013.11.10 - 2013.11.12

Language：Japanese Presentation type：Oral presentation (general)

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Rab18はC型肝炎ウイルスの感染性粒子形成に重要な宿主因子である

團迫浩方他

第61回日本ウイルス学会学術集会

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Event date： 2013.11.10 - 2013.11.12

Language：Japanese Presentation type：Oral presentation (general)

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ヒト肝細胞株におけるHBV複製能の評価

奥村暢章、池田正徳、武田緑、佐藤伸哉、團迫浩方、溝上雅史、加藤宣之

第61回日本ウイルス学会学術集会

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Event date： 2013.11.10 - 2013.11.12

Language：Japanese Presentation type：Oral presentation (general)

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C型肝炎ウイルスのライフサイクルにおける宿主因子annexinA1の役割

平本洸貴、團迫浩方、瀬島寛恵、森京子、佐藤伸哉、池田正徳、脇田隆字、加藤宣之之

第61回日本ウイルス学会学術集会

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Event date： 2013.11.10 - 2013.11.12

Language：Japanese Presentation type：Oral presentation (general)

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リバビリン耐性全長HCV-RNA複製細胞株の樹立

佐藤伸哉、森京子、上田優輝、瀬島寛恵、團迫浩方、池田正徳、加藤宣之

第61回日本ウイルス学会学術集会

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Event date： 2013.11.10 - 2013.11.12

Language：Japanese Presentation type：Oral presentation (general)

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HBV複製系の開発に向けた肝細胞株の選択

奥村暢章、池田正徳、武田緑、團迫浩方、加藤宣之

第22回日本消化器関連学会週間

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Event date： 2013.10.9 - 2013.10.10

Language：Japanese Presentation type：Poster presentation

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Identification of two microRNAs showing decreased expression in cell-based HCV RNA replication for 2years International conference

Hiroe Sejima,Kyoko Mori,Shinya Satoh,Hiromichi Dansako,Masanori Ikeda,Nobuyuki Kato

20th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2013.10.6 - 2013.10.10

Language：English Presentation type：Poster presentation

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Raloxifene inhibits hepatitis C virus infection and replication International conference

Midori Takeda,Masanori Ikeda,Kyoko Mori,Masahiko Yano,Yasuo Ariumi,Hiromichi Dansako,Takaji Wakita,Nobuyuki Kato

20th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2013.10.6 - 2013.10.10

Language：English Presentation type：Poster presentation

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Rab18 is required for viral assembly of hepatitis C virus through the association with core protein around lipid droplet. International conference

Hiromichi Dansako他

20th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2013.10.6 - 2013.10.10

Language：English Presentation type：Poster presentation

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Establishment of hepatitis C virus RNA replicating cell lines possessing ribavirin-resistant phenotypes International conference

Shinya Satoh,Kyoko Mori,Hiromichi Dansako,Masanori Ikeda,Nobuyuki Kato

20th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2013.10.6 - 2013.10.10

Language：English Presentation type：Poster presentation

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Anti-HCV activity of Cordyceps militaris used as a chinese herbal medicine International conference

Youki Ueda,Kyoko Mori,Shinya Satoh,Hiromichi Dansako,Masanori Ikeda,Nobuyuki Kato

20th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2013.10.6 - 2013.10.10

Language：English Presentation type：Poster presentation

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Identification of microRNAs showing differential expression profiles in cell-based HCV RNA replication for 2years

瀬島寛恵、森京子、佐藤伸哉、團迫浩方、池田正徳、加藤宣之

第72回日本癌学会学術集会

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Event date： 2013.10.3 - 2013.10.5

Language：Japanese Presentation type：Poster presentation

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骨粗鬆症治療剤であるラロキシフェンは抗HCV活性を示す

武田緑、池田正徳、森京子、矢野雅彦、有海康雄、團迫浩方、脇田隆字、加藤宣之

第28回中国四国ウイルス研究会

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Event date： 2013.6.22 - 2013.6.23

Language：Japanese Presentation type：Oral presentation (general)

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肝細胞株におけるHBV複製能の評価

奥村暢章、池田正徳、武田緑、佐藤伸哉、團迫浩方、加藤宣之

第28回中国四国ウイルス研究会

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Event date： 2013.6.22 - 2013.6.23

Language：Japanese Presentation type：Oral presentation (general)

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B型肝炎ウイルスの自然免疫応答モデルの構築

團迫浩方他

第28回中国四国ウイルス研究会

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Event date： 2013.6.22 - 2013.6.23

Language：Japanese Presentation type：Oral presentation (general)

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C型肝炎ウイルスの感染性ウイルス粒子産生に対するannexin A1の影響

平本洸貴、團迫浩方、瀬島寛恵、佐藤伸哉、池田正徳、加藤宣之

第28回中国四国ウイルス研究会

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Event date： 2013.6.22 - 2013.6.23

Language：Japanese Presentation type：Oral presentation (general)

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2年間にわたるC型肝炎ウイルスのゲノム複製によって発現レベルに変動をきたしたmicroRNAの同定

瀬島寛恵、森京子、團迫浩方、池田正徳、加藤宣之

第28回中国四国ウイルス研究会

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Event date： 2013.6.22 - 2013.6.23

Language：Japanese Presentation type：Oral presentation (general)

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中国由来の漢方薬であるサナギタケ（北冬虫夏草）が有する抗HCV活性

上田優輝、森京子、團迫浩方、池田正徳、加藤宣之

第28回中国四国ウイルス研究会

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Event date： 2013.6.22 - 2013.6.23

Language：Japanese Presentation type：Oral presentation (general)

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中国由来の漢方薬であるサナギタケ（北冬虫夏草）に見出された抗HCV活性

上田優輝、森京子、團迫浩方、池田正徳、加藤宣之

第49回日本肝臓学会総会

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Event date： 2013.6.6 - 2013.6.7

Language：Japanese Presentation type：Poster presentation

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骨粗鬆症治療剤であるラロキシフェンの抗HCV活性機構について

武田緑、池田正徳、森京子、矢野雅彦、有海康雄、團迫浩方、脇田隆字、加藤宣之

第49回日本肝臓学会総会

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Event date： 2013.6.6 - 2013.6.7

Language：Japanese Presentation type：Oral presentation (keynote)

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C型肝炎ウイルスの複製環境に関わるmicroRNAの探索

瀬島寛恵、森京子、團迫浩方、池田正徳、加藤宣之

第49回日本肝臓学会総会

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Event date： 2013.6.6 - 2013.6.7

Language：Japanese Presentation type：Poster presentation

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酸化ストレスを介して強い抗HCV活性を示す抗マラリア薬として開発中の化合物

上田優輝、森京子、團迫浩方、金惠淑、綿矢有祐、池田正徳、加藤宣之

第60回日本ウイルス学会学術集会

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Event date： 2012.11.13 - 2012.11.15

Language：Japanese Presentation type：Oral presentation (general)

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リバビリンに対する感受性を決定する宿主因子の同定とその因子の病態進行への関与について

森京子、本多政夫、池田正徳、團迫浩方、金子周一、加藤宣之

第60回日本ウイルス学会学術集会

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Event date： 2012.11.13 - 2012.11.15

Language：Japanese Presentation type：Oral presentation (general)

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Potent anti-HCV activities found in preclinical anti-malarial drugs is promptly exerted through oxidative stress International conference

Youki Ueda,Kyoko Mori,Hiromichi Dansako,Hye-Sook Kim,Yusuke Wataya,Masanori Ikeda,Nobuyuki Kato

19th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2012.10.5 - 2012.10.9

Language：English Presentation type：Poster presentation

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Multidirectional analysis for a host factor determining the anti-HCV activity of ribavirin International conference

Kyoko Mori,Masao Honda,Masanori Ikeda,Hiromichi Dansako,Shuichi Kaneko,Nobuyuki Kato

19th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2012.10.5 - 2012.10.9

Language：English Presentation type：Poster presentation

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Multifaceted analysis for a host factor determining the anti-HCV activity of ribavirin

Kyoko Mori,Masao Honda,Masanori Ikeda,Hiromichi Dansako,Shuichi Kaneko,Nobuyuki Kato

第71回日本癌学会学術総会

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Event date： 2012.9.19 - 2012.9.21

Language：English Presentation type：Poster presentation

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Potent anti-HCV activities found in preclinical anti-malarial drugs

Youki Ueda,Kyoko Mori,Hiromichi Dansako,Hye-Sook Kim,Yusuke Wataya,Masanori Ikeda,Nobuyuki Kato

第71回日本癌学会学術総会

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Event date： 2012.9.19 - 2012.9.21

Language：English Presentation type：Poster presentation

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クラスAスカベンジャー受容体によるC型肝炎ウイルスの新規感染認識機構の解明

團迫浩方、山根大典、加藤宣之、Stanley.M.Lemon

第27回中国四国ウイルス研究会

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Event date： 2012.6.23 - 2012.6.24

Language：Japanese Presentation type：Oral presentation (general)

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HCV production requires the PML tumor suppressor protein International conference

Misao Kuroki,Yasuo Ariumi,Masanori Ikeda,Hiromichi Dansako,Takaji Wakita,Nobuyuki Kato

18th INTERNATIONAL SYMPOSIUM ON HEPATITIS C VIRUS AND RELATED VIRUSES

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Event date： 2011.9.8 - 2011.9.12

Language：English Presentation type：Poster presentation

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IL28B領域のSNPが異なる肝細胞株(HuH7,Li23)における抗HCV剤の感受性

池田正徳、森京子、武田緑、中澤貴秀、有海康雄、團迫浩方、加藤宣之

第33回日本分子生物学会年会

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Event date： 2010.12.7 - 2010.12.10

Language：Japanese Presentation type：Poster presentation

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リバビリンの抗HCV活性を決定する因子の解析

森京子、池田正徳、有海康雄、團迫浩方、脇田隆字、加藤宣之

第58回日本ウイルス学会学術集会

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Event date： 2010.11.7 - 2010.11.9

Language：Japanese Presentation type：Oral presentation (general)

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異なるHCV株、細胞株を用いたHCV RNA複製培養細胞での薬剤評価

池田正徳、森京子、武田緑、中澤貴秀、有海康雄、團迫浩方、加藤宣之

第58回日本ウイルス学会学術集会

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Event date： 2010.11.7 - 2010.11.9

Language：Japanese Presentation type：Oral presentation (general)

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がん抑制因子PMLはC型肝炎ウイルスのライフサイクルに必須である

黒木美沙緒、有海康雄、池田正徳、團迫浩方、脇田隆字、加藤宣之

第58回日本ウイルス学会学術集会

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Event date： 2010.11.7 - 2010.11.9

Language：Japanese Presentation type：Oral presentation (general)

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ヒト肝癌細胞株Li23由来の新しいHCV-RNA複製システムを用いたリバビリンの作用機序の解明

森京子、池田正徳、有海康雄、團迫浩方、加藤宣之

第14回日本肝臓学会大会

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Event date： 2010.10.13

Language：Japanese Presentation type：Poster presentation

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C型肝炎ウイルスに対する新しい抗ウイルス剤スクリーニング系の開発

池田正徳、森京子、中澤貴秀、有海康雄、團迫浩方、加藤宣之

第14回日本肝臓学会大会

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Event date： 2010.10.13

Language：Japanese Presentation type：Poster presentation

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Mechanism of anti-HCV of ribavirin in a novel HCV replication cell system

森京子、池田正徳、有海康雄、團迫浩方、加藤宣之

第69回日本癌学会学術総会

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Event date： 2010.9.22 - 2010.9.24

Language：Japanese Presentation type：Oral presentation (general)

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The PML tumor suppressor protein is required for HCV life

黒木美沙緒、有海康雄、池田正徳、團迫浩方、脇田隆字、加藤宣之

第69回日本癌学会学術総会

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Event date： 2010.9.22 - 2010.9.24

Language：Japanese Presentation type：Oral presentation (general)

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Development of genome-length HCV RNA replication assay systems derived from different HCV strains using HuH-7 and Li23 cells International conference

17th International Meeting on Hepatitis C Virus and Related Viruses

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Event date： 2010.9.10 - 2010.9.14

Language：English Presentation type：Poster presentation

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Anti-HCV mechanism of ribavirin in novel HCV replication cell systems International conference

17th International Meeting on Hepatitis C Virus and Related Viruses

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Event date： 2010.9.10 - 2010.9.14

Language：English Presentation type：Poster presentation

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Li23細胞由来のHCV-RNA複製システムを用いたリバビリンの抗HCV機構の解析

森京子、池田正徳、有海康雄、團迫浩方、加藤宣之

中国四国ウイルス研究会

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Event date： 2010.6.26 - 2010.6.27

Language：Japanese Presentation type：Oral presentation (general)

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がん抑制因子PMLはC型肝炎ウイルスの生活環に必要である

黒木美沙緒、有海康雄、池田正徳、團迫浩方、脇田隆字、加藤宣之

中国四国ウイルス研究会

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Event date： 2010.6.26 - 2010.6.27

Language：Japanese Presentation type：Oral presentation (general)

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癌抑制因子PMLはHCV粒子産生に必要である

黒木美沙緒、有海康雄、池田正徳、團迫浩方、脇田隆字、加藤宣之

第57回日本ウイルス学会学術集会

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Event date： 2009.10.25 - 2009.10.27

Language：Japanese Presentation type：Oral presentation (general)

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オンコスタチンMはインターフェロンの抗HCV活性を相乗的に増強する

池田正徳、森京子、有海康雄、團迫浩方、加藤宣之

第57回日本ウイルス学会学術集会

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Event date： 2009.10.25 - 2009.10.27

Language：Japanese Presentation type：Oral presentation (general)

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リバビリンの抗HCV活性を解析評価できるLi23細胞由来のHCV-RNA複製システム

森京子、池田正徳、有海康雄、團迫浩方、加藤宣之

第57回日本ウイルス学会学術集会

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Event date： 2009.10.25 - 2009.10.27

Language：Japanese Presentation type：Oral presentation (general)

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ESCRT小胞輸送系のHCV産生への関与

有海康雄、黒木美沙緒、牧正敏、池田正徳、團迫浩方、脇田隆字、加藤宣之

第57回日本ウイルス学会学術集会

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Event date： 2009.10.25 - 2009.10.27

Language：Japanese Presentation type：Oral presentation (general)

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抗潰瘍剤によるC型慢性肝炎の新たな治療戦略-TeprenoneはStatinのゲラニルゲラニル化阻害を増強しHCV複製抑制効果を増強する-

河合良成、池田正徳、矢野雅彦、阿部健一、西村剛、團迫浩方、有海康雄、脇田隆字、山本和秀、加藤宣之

第57回日本ウイルス学会学術集会

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Event date： 2009.10.25 - 2009.10.27

Language：Japanese Presentation type：Poster presentation

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HCVコア蛋白質のアミノ酸の違いとIFN応答性との関係についての培養細胞を用いた解析

池田房雄、團迫浩方、西村剛、河合良成、有海康雄、池田正徳、高木章乃夫、岩崎良章、加藤宣之、山本和秀

第13回日本肝臓学会大会

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Event date： 2009.10.14 - 2009.10.16

Language：Japanese Presentation type：Poster presentation

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The ESCRT pathway is required for HCV production. International conference

16th International Meeting on Hepatitis C Virus and Related Viruses

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Event date： 2009.10.3 - 2009.10.7

Language：English Presentation type：Oral presentation (general)

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Replicons from genotype 1b HCV-positive sera exhibit diverse sensitivities to anti-HCV reagents. International conference

16th International Meeting on Hepatitis C Virus and Related Viruses

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Event date： 2009.10.3 - 2009.10.7

Language：English Presentation type：Poster presentation

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The PML tumor suppressor protein is required for HCV production. International conference

16th International Meeting on Hepatitis C Virus and Related Viruses

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Event date： 2009.10.3 - 2009.10.7

Language：English Presentation type：Poster presentation

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Involvement of the MEK-ERK1/2 Signaling Pathway in the Anti-HCV Mechanism of Oxidative Stress. International conference

16th International Meeting on Hepatitis C Virus and Related Viruses

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Event date： 2009.10.3 - 2009.10.7

Language：English Presentation type：Poster presentation

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Li23 cell-derived HCV-RNA replicating systems enabling analysis for anti-HCV mechanism of ribavirin. International conference

16th International Meeting on Hepatitis C Virus and Related Viruses

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Event date： 2009.10.3 - 2009.10.7

Language：English Presentation type：Poster presentation

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Anti-ulcer agent, teprenone, enhanced statin’s anti-HCV activity by augmenting theinhibition of geranylgeranylation. International conference

16th International Meeting on Hepatitis C Virus and Related Viruses

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Event date： 2009.10.3 - 2009.10.7

Language：English Presentation type：Poster presentation

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Oncostatin M synergistically inhibits HCV RNA replication in combination with interferon-a. International conference

16th International Meeting on Hepatitis C Virus and Related Viruses

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Event date： 2009.10.3 - 2009.10.7

Language：English Presentation type：Poster presentation

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抗潰瘍剤によるC型慢性肝炎の新たな治療戦略-Teprenone(Selbex?)とPlaunotol(Kelnac?)のHCV複製抑制効果-

河合良成、池田正徳、森京子、阿部健一、矢野雅彦、池田房雄、有海康雄、團迫浩方、山本和秀、加藤宣之

第24回中国四国ウイルス研究会

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Event date： 2009.7.4 - 2009.7.5

Language：Japanese Presentation type：Oral presentation (general)

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亜ヒ酸は酸化ストレスとグルタチオンレドックスシステムを介してC型肝炎ウイルス(HCV)RNA複製を抑制する

黒木美沙緒、有海康雄、池田正徳、團迫浩方、脇田隆字、加藤宣之

第24回中国四国ウイルス研究会

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Event date： 2009.7.4 - 2009.7.5

Language：Japanese Presentation type：Oral presentation (general)

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C型急性肝炎患者由来の全長HCV-RNA複製細胞株の樹立とその応用

森京子、阿部健一、團迫浩方、有海康雄、池田正徳、加藤宣之

第24回中国四国ウイルス研究会

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Event date： 2009.7.4 - 2009.7.5

Language：Japanese Presentation type：Oral presentation (general)

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抗潰瘍剤によるC型慢性肝炎の新たな治療戦略-Teprenone(Selbex?)とPlaunotol(Kelnac?)のHCV複製抑制効果-

河合良成、池田正徳、森京子、阿部健一、矢野雅彦、池田房雄、有海康雄、團迫浩方、山本和秀、加藤宣之

第45回日本肝臓学会総会

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Event date： 2009.6.4 - 2009.6.5

Language：Japanese Presentation type：Poster presentation

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日常的に摂取し抗HCV効果を有する栄養成分の全長HCV RNA複製細胞を用いた網羅的探索

矢野雅彦、池田正徳、阿部健一、*團迫浩方、大越章吾、青柳豊、加藤宣之

第５５回日本ウイルス学会学術集会・総会

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Event date： 2007.10.21 - 2007.10.23

Presentation type：Poster presentation

Venue：札幌市

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HCVのRNA複製に必要な宿主因子DDX3 DEAD box RNAへリカーゼ。

有海康雄、黒木美沙緒、阿部健一、*團迫浩方、池田正徳、脇田隆字、加藤宣之

第５５回日本ウイルス学会学術集会・総会

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Event date： 2007.10.21 - 2007.10.23

Presentation type：Oral presentation (general)

Venue：札幌市

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DNA損傷センサーATMとChk2はHCVのRNA複製に必要な宿主因子である。

有海康雄、黒木美沙緒、阿部健一、*團迫浩方、池田正徳、脇田隆字、トロノディディエ、加藤宣之

第５５回日本ウイルス学会学術集会・総会

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Event date： 2007.10.21 - 2007.10.23

Presentation type：Oral presentation (general)

Venue：札幌市

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C型急性肝炎患者由来の1b型HCVレプリコン複製細胞株の樹立

森京子、阿部健一、*團迫浩方、有海康雄、池田正徳、加藤宣之

第５５回日本ウイルス学会学術集会・総会

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Event date： 2007.10.21 - 2007.10.23

Presentation type：Poster presentation

Venue：札幌市

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全長HCV-RNAの複製レベルを指標として生細胞のままアッセイできる新しい抗ウイルス剤評価システム

*團迫浩方、池田正徳、阿部健一、森京子、有海康雄、加藤宣之

第５５回日本ウイルス学会学術集会・総会

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Event date： 2007.10.21 - 2007.10.23

Presentation type：Oral presentation (general)

Venue：札幌市

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全長HCV-RNA複製細胞の長期培養に生じるHCVの遺伝的多様性

加藤宣之、阿部健一、森京子、有海康雄、*團迫浩方、池田正徳

第５５回日本ウイルス学会学術集会・総会

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Event date： 2007.10.21 - 2007.10.23

Presentation type：Poster presentation

Venue：札幌市

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HCV genetic variation and dynamics in long-term culture of genome-length HCV RNA replication cells.

Nobuyuki Kato, Ken-ichi Abe, Kyoko Mori, Yasuo Ariumi, *Hiromichi Dansako, Masanori Ikeda

第６６回日本癌学会総会

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Event date： 2007.10.3 - 2007.10.5

Presentation type：Poster presentation

Venue：横浜市

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DDX3 DEAD box RNA helicase is required for hepatitis C virus RNA replication.

Yasuo Ariumi, Misao Kuroki, Ken-ichi Abe, *Hiromichi Dansako, Masanori Ikeda, Takaji Wakita, Nobuyuki Kato

第６６回日本癌学会総会

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Event date： 2007.10.3 - 2007.10.5

Presentation type：Oral presentation (general)

Venue：横浜市

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Comprehensive screening of ordinary nutrients expected to enhance the effects of anti-HCV reagents.

Masanori Ikeda, Masahiko Yano, Ken-ichi Abe, *Hiromichi Dansako, Shogo Ohkoshi, Yutaka Aoyagi, Nobuyuki Kato

第６６回日本癌学会総会

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Event date： 2007.10.3 - 2007.10.5

Presentation type：Oral presentation (general)

Venue：横浜市

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HCV genetic variability and dynamics in long-term culture of genome-length HCV RNA-replicating cells. International conference

Nobuyuki Kato, Ken-ichi Abe, Kyoko Mori, Yasuo Ariumi, *Hiromichi Dansako, Masanori Ikeda

14 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2007.9.9 - 2007.9.13

Presentation type：Poster presentation

Venue：Glasgow, Scotland UK

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DNA damage sensors, ATM and Chk2, are required for hepatitis C virus RNA replication. International conference

Yasuo Ariumi, Misao Kuroki, Ken-ichi Abe, *Hiromichi Dansako, Masanori Ikeda, Takaji Wakita, Didier Trono, Nobuyuki Kato

14 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2007.9.9 - 2007.9.13

Presentation type：Poster presentation

Venue：Glasgow, Scotland UK

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Serum-free cell culture system supplemented with lipid-rich albumin for genome-length HCV RNA replication. International conference

Ken-ichi Abe, Masanori Ikeda, Yasuo Ariumi, *Hiromichi Dansako, Nobuyuki Kato

14 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2007.9.9 - 2007.9.13

Presentation type：Poster presentation

Venue：Glasgow, Scotland UK

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Comprehensive analysis of the ordinary nutrients as the novel partners of anti-HCV reagents. International conference

Masahiko Yano, Masanori Ikeda, Ken-ichi Abe, *Hiromichi Dansako, Shogo Ohkoshi, Yutaka Aoyagi, Nobuyuki Kato

14 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2007.9.9 - 2007.9.13

Presentation type：Poster presentation

Venue：Glasgow, Scotland UK

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A new antiviral assay system using living cells harboring genome-length HCV RNA. International conference

*Hiromichi Dansako, Masanori Ikeda, Ken-ichi Abe, Kyoko Mori, Yasuo Ariumi, Nobuyuki Kato

14 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2007.9.9 - 2007.9.13

Presentation type：Poster presentation

Venue：Glasgow, Scotland UK

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Limited suppression of the interferon-β production by hepatitis C virus serine protease in cultured human hepatocytes.

*Hiromichi Dansako, Masanori Ikeda, Nobuyuki Kato

第72回日本インターフェロン・サイトカイン学会学術集会

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Event date： 2007.7.5 - 2007.7.6

Presentation type：Poster presentation

Venue：京都市

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持続的な全長HCV RNA複製を維持できる無血清培地を用いた細胞培養系の開発

阿部健一,池田正徳,有海康雄,*團迫浩方,加藤宣之

第23回中国四国ウイルス研究会

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Event date： 2007.6.16 - 2007.6.17

Presentation type：Oral presentation (general)

Venue：松山市

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C型急性肝炎患者血清由来のHCV 1bレプリコン複製細胞株の樹立

森京子,阿部健一,*團迫浩方,有海康雄,池田正徳,加藤宣之

第23回中国四国ウイルス研究会

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Event date： 2007.6.16 - 2007.6.17

Presentation type：Oral presentation (general)

Venue：松山市

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全長HCV RNAの複製に影響を与える日常的に摂取する栄養成分の同定および評価

矢野雅彦,池田正徳,阿部健一,*團迫浩方,大越章吾,青柳豊,加藤宣之

第23回中国四国ウイルス研究会

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Event date： 2007.6.16 - 2007.6.17

Presentation type：Oral presentation (general)

Venue：松山市

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抗HCV剤治療効果の増強が期待される物質の網羅的スクリーニング-C型肝炎に対する治療戦略の最大化を目指して-.

矢野雅彦,池田正徳,阿部健一,*團迫浩方,大越章吾,青柳豊,加藤宣之

第43回日本肝臓学会総会

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Event date： 2007.5.31 - 2007.6.1

Presentation type：Oral presentation (general)

Venue：東京

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ヒト肝癌由来の培養細胞におけるC型肝炎ウイルスおよびエタノールのTGF-β産生に及ぼす影響に関する解析

山田将士,池田正徳,阿部健一,*團迫浩方,加藤宣之

第54回日本ウイルス学会学術集会・総会

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Event date： 2006.11.19 - 2006.11.21

Venue：名古屋市

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C型肝炎ウイルスNS3-4A蛋白質のインターフェロン-β産生阻害メカニズム

* 團迫浩方,池田正徳,加藤宣之

第54回日本ウイルス学会学術集会・総会

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Event date： 2006.11.19 - 2006.11.21

Venue：名古屋市

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全長HCV RNAの効率的な複製を引き起こすNS3領域の適応変異に関する解析

阿部健一,池田正徳,*團迫浩方,加藤宣之

第54回日本ウイルス学会学術集会・総会

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Event date： 2006.11.19 - 2006.11.21

Venue：名古屋市

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1b型と2a型HCVのシスエレメント領域のキメラウイルスを用いたウイルスの複製および粒子産生に関する解析

池田正徳,阿部健一,*團迫浩方,脇田隆宇,加藤宣之

第54回日本ウイルス学会学術集会・総会

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Event date： 2006.11.19 - 2006.11.21

Venue：名古屋市

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全長HCV RNAの複製を長期間維持できる無血清培地を用いた細胞培養系の開発

阿部健一,池田正徳,*團迫浩方,加藤宣之

第54回日本ウイルス学会学術集会・総会

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Event date： 2006.11.19 - 2006.11.21

Venue：名古屋市

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HCV RNAの複製を生細胞のまま観察できる培養システムの開発

*團迫浩方,池田正徳,阿部健一,加藤宣之

第54回日本ウイルス学会学術集会・総会

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Event date： 2006.11.19 - 2006.11.21

Venue：名古屋市

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HCV-O (1b) とJFH1 (2a) のシスエレメントキメラウイルスの作成とそれらを用いたHCV複製効率規定領域の解析

池田正徳,阿部健一,*團迫浩方,脇田隆宇,加藤宣之

第65回日本癌学会総会

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Event date： 2006.9.28 - 2006.9.30

Venue：横浜市

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HCV RNAの複製レベルを生細胞のまま定量できる新しい評価システムの開発

*團迫浩方,池田正徳,阿部健一,加藤宣之

第65回日本癌学会総会

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Event date： 2006.9.28 - 2006.9.30

Venue：横浜市

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HCV RNAの複製を著しく亢進させる適応変異の組合せの同定

阿部健一,池田正徳,*團迫浩方,仲一仁,加藤宣之

第65回日本癌学会総会

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Event date： 2006.9.28 - 2006.9.30

Venue：横浜市

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Comparative analysis of inhibiting activities against IFN-beta production of NS3-4As derived from patients with different hepatic conditions.

*Hiromichi Dansako, Kazunori Takemoto, Kazuhito Naka, Masanori Ikeda, Nobuyuki Kato

13 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2006.8.27 - 2006.8.31

Venue：Cairns, Australia

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Development of a new cell-based assay for monitoring HCV RNA replication level with living cells.

*Hiromichi Dansako, Masanori Ikeda, Ken-ichi Abe, Nobuyuki Kato

13 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2006.8.27 - 2006.8.31

Venue：Cairns, Australia

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Tandem repeats of lactoferrin-derived anti-HCV peptide enhance the antiviral activity.

Ken-ichi Abe, Akito Nozaki, Kazushi Tamura, Masanori Ikeda, Kazuhito Naka, *Hiromichi Dansako, Hiro-o Hoshino, Nobuyuki Kato

13 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2006.8.27 - 2006.8.31

Venue：Cairns, Australia

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Combination of cell culture-adaptivemutations causes the drastic enhancement of genome-length HCV RNA replication.

Ken-ichi Abe, Masanori Ikeda, *Hiromichi Dansako, Kazuhito Naka, Nobuyuki Kato

13 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2006.8.27 - 2006.8.31

Venue：Cairns, Australia

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Production and characterization of infectious HCVs from JFH1 (2a) with cis-element of HCV-O (1b).

Masanori Ikeda, Ken-ichi Abe, *Hiromichi Dansako, Takaji Wakita, Nobuyuki Kato

13 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2006.8.27 - 2006.8.31

Venue：Cairns, Australia

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全長HCV RNAの効率的な複製を引き起こす適応変異に関する解析

阿部健一,池田正徳,*團迫浩方,仲一仁,加藤宣之

第22回中国四国ウイルス研究会

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Event date： 2006.6.10 - 2006.6.11

Venue：鳥取市

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生細胞のままHCV RNA複製を観察できるシステムの開発

*團迫浩方,池田正徳,阿部健一,加藤宣之

第22回中国四国ウイルス研究会

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Event date： 2006.6.10 - 2006.6.11

Venue：鳥取市

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C型肝炎ウイルスおよびエタノールのTGF-b産生に及ぼす影響について

山田将士,池田正徳,阿部健一,*團迫浩方,加藤宣之

第22回中国四国ウイルス研究会

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Event date： 2006.6.10 - 2006.6.11

Venue：鳥取市

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C型肝炎ウイルスレプリコン細胞のインターフェロン抵抗性獲得機構

加藤宣之,阿部健一,竹本和憲,*團迫浩方,池田正徳,下遠野邦忠

第53回日本ウイルス学会学術集会・総会

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Event date： 2005.11.20 - 2005.11.22

Venue：横浜市

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C型肝炎ウイルス蛋白質のインターフェロン-β産生系に対する相反的効果

*團迫浩方,池田正徳,加藤宣之

第53回日本ウイルス学会学術集会・総会

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Event date： 2005.11.20 - 2005.11.22

Venue：横浜市

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全長HCV RNA複製抑制効果を示すスタチンの種類による抗HCV効果の違いとインターフェロンとの併用による相乗的抗HCV効果

池田正徳,阿部健一,山田将士,*團迫浩方,加藤宣之

第53回日本ウイルス学会学術集会・総会

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Event date： 2005.11.20 - 2005.11.22

Venue：横浜市

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各種病態由来のC型肝炎ウイルスNS3-4A： IFN-β産生抑制効果の比較

竹本和憲,*團迫浩方,池田正徳,加藤宣之

第53回日本ウイルス学会学術集会・総会

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Event date： 2005.11.20 - 2005.11.22

Venue：横浜市

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HCV-O株由来の全長HCV RNA複製細胞群の樹立と適応変異に関する解析

阿部健一,池田正徳,*團迫浩方,加藤宣之

第53回日本ウイルス学会学術集会・総会

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Event date： 2005.11.20 - 2005.11.22

Venue：横浜市

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HCV proteins exhibit contradictory effects on interferon system in human hepatocyte cells.

*Hiromichi Dansako, Kazuhito Naka, Masanori Ikeda, Nobuyuki Kato

12 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2005.10.2 - 2005.10.6

Venue：Montreal, Canada

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HMG-CoA reductase inhibitors as a new class of anti-HCV reagents: their different effects on HCV replication and their applications for combination therapy with IFN.

Masanori Ikeda, Ken-ichi Abe, Masashi Yamada, *Hiromichi Dansako, Kazuhito Naka, Nobuyuki Kato

12 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2005.10.2 - 2005.10.6

Venue：Montreal, Canada

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Identification of a cell culture-adaptive mutation in newly established genome-length HCV RNA replicating cell lines.

Ken-ichi Abe, Masanori Ikeda, *Hiromichi Dansako, Kazuhito Naka, Nobuyuki Kato

12 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2005.10.2 - 2005.10.6

Venue：Montreal, Canada

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Interferon resistance of HCV replicons is caused by functional disruption of type I interferon receptors.

Kazuhito Naka, Kazunori Takemoto, Ken-ichi Abe, *Hiromichi Dansako, Masanori Ikeda, Kunitada Shimotohno, Nobuyuki Kato

12 th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2005.10.2 - 2005.10.6

Venue：Montreal, Canada

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全長HCV RNAレポーター評価システムにより見出したスタチンとインターフェロンの併用による相乗的抗ウイルス効果

池田正徳,阿部健一,*團迫浩方,仲一仁,加藤宣之

第64回日本癌学会総会

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Event date： 2005.9.14 - 2005.9.16

Venue：札幌市

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HCV-O株由来の全長HCV RNA複製細胞群の樹立

阿部健一,池田正徳,*團迫浩方,仲一仁,加藤宣之

第64回日本癌学会総会

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Event date： 2005.9.14 - 2005.9.16

Venue：札幌市

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各種病態由来のHCV NS3-4A：それらの遺伝子解析とIFN-b産生抑制効果の比較

竹本和憲,仲一仁,*團迫浩方,池田正徳,加藤宣之

第21回中国四国ウイルス研究会

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Event date： 2005.6.18 - 2005.6.19

Venue：岡山県倉敷市

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HCV-O株由来の全長HCV RNA複製細胞群の樹立

阿部健一,池田正徳,*團迫浩方,仲一仁,加藤宣之

第21回中国四国ウイルス研究会

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Event date： 2005.6.18 - 2005.6.19

Venue：岡山県倉敷市

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C型肝炎ウイルス(HCV)レプリコン細胞および全長HCV RNA複製細胞を用いたcDNAマイクロアレイ解析

阿部健一,池田正徳,*團迫浩方,仲一仁,下遠野邦忠,加藤宣之

第52回日本ウイルス学会学術集会・総会

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Event date： 2004.11.21 - 2004.11.23

Venue：横浜市

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HCV-O(1B-2)株由来の全長HCV RNA複製系の開発

池田正徳,阿部健一,*團迫浩方,中村孝志,仲一仁,加藤宣之

第52回日本ウイルス学会学術集会・総会

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Event date： 2004.11.21 - 2004.11.23

Venue：横浜市

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C型肝炎ウイルスNS5B蛋白質によるTLR3シグナル経路の活性化

仲一仁,*團迫浩方,小林直哉,池田正徳,加藤宣之

第52回日本ウイルス学会学術集会・総会

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Event date： 2004.11.21 - 2004.11.23

Venue：横浜市

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Distraction of interferon signaling pathway in non-cancerous hepatocytes by HCV proteins.

*Hiromichi Dansako, Kazuhito Naka, Naoya Kobayashi, Masanori Ikeda and Nobuyuki Kato

11th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2004.10.3 - 2004.10.7

Venue：Heidelberg, Germany

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Hepatitis C virus NS5B avtivates TLR3 signaling pathway in non-cancerous hepatocytes.

Kazuhito Naka, *Hiromichi Dansako, Naoya Kobayashi, Masanori Ikeda and Nobuyuki Kato

11th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2004.10.3 - 2004.10.7

Venue：Heidelberg, Germany

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cDNA microarray analysis to compare HCV subgenomic and genome-length RNA replicating cells with their cured cells.

Ken-ichi Abe, Masanori Ikeda, *Hiromichi Dansako, Kazuhito Naka, Kunitada Shimotohno and Nobuyuki Kato

11th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2004.10.3 - 2004.10.7

Venue：Heidelberg, Germany

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Characterization of cured cells derived from a clonal Huh-7 cell line replicating a newly established genome-length HCV RNA(HCV-O).

Masanori Ikeda, Ken-ichi Abe, *Hiromichi Dansako, Takashi Nakamura, Kazuhito Naka and Nobuyuki Kato

11th International Symposium on Hepatitis C Virus and Related Viruses

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Event date： 2004.10.3 - 2004.10.7

Venue：Heidelberg, Germany

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C型肝炎ウイルスレプリコン細胞を用いたcDNAマイクロアレイ解析

阿部健一,池田正徳,*團迫浩方,仲一仁,下遠野邦忠,加藤宣之

第20回中国四国ウイルス研究会

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Event date： 2004.6.26 - 2004.6.27

Venue：高知市

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C型肝炎ウイルス蛋白質によるインターフェロンシグナル伝達経路の活性化

*團迫浩方,仲一仁,池田正徳,加藤宣之

第20回中国四国ウイルス研究会

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Event date： 2004.6.26 - 2004.6.27

Venue：高知市

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Establishment of a hepatitis C virus subgenomic replicon derived from human hepatocytes infected in vitro.

Nobuyuki Kato, Kazuo Sugiyama, Katsuyuki Namba, *Hiromichi Dansako, Takashi Nakamura, Kazuhito Naka, Akito Nozaki snd Kunitada Shimotohno

10 th International Meeting of Hepatitis C Virus and Related Viruses

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Event date： 2003.12.2 - 2003.12.6

Venue：Kyoto, Japan

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Differential activation of interferon-inducible genes by hepatitis C virus core protein mediated by the interferon stimulated response element.

*Hiromichi Dansako, Atsushi Naganuma, Takashi Nakamura, Akito Nozaki and Nobuyuki Kato

10 th International Meeting of Hepatitis C Virus and Related Viruses

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Event date： 2003.12.2 - 2003.12.6

Venue：Kyoto, Japan

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Establishment of interferon-resistant hepatitis C virus subgenomic replicons.

Kazuhito Naka, Katsuyuki Namba, Takashi Nakamura, *Hiromichi Dansako, Akito Nozaki, Kunitada Shimotohno and Nobuyuki Kato

10 th International Meeting of Hepatitis C Virus and Related Viruses

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Event date： 2003.12.2 - 2003.12.6

Venue：Kyoto, Japan

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Genetic evolution of hepatitis C virus subgenomic replicon in long-term culture.

Nobuyuki Kato, Takashi Nakamura, *Hiromichi Dansako, Katsuyuki Namba, Takahiko Tamura, Akito Nozaki, Kazuhito Naka and Kunitada Shimotohno

10 th International Meeting of Hepatitis C Virus and Related Viruses

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Event date： 2003.12.2 - 2003.12.6

Venue：Kyoto, Japan

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長期培養におけるC型肝炎ウイルスsubgenomic repliconの遺伝的変異と多様性

中村孝志,*團迫浩方,難波克行,田村隆彦,仲一仁,野崎昭人,下遠野邦忠,加藤宣之

第51回日本ウイルス学会学術集会・総会

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Event date： 2003.10.27 - 2003.10.29

Venue：京都市

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C型肝炎ウイルス（HCV）感染ヒト肝細胞由来の新規HCVsubgenomic replicon細胞の樹立

加藤宣之,杉山和夫,難波克行,*團迫浩方,中村孝志,仲一仁,野崎昭人,下遠野邦忠

第51回日本ウイルス学会学術集会・総会

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Event date： 2003.10.27 - 2003.10.29

Venue：京都市

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C型肝炎ウイルス蛋白質によるインターフェロンシグナル伝達系の活性化

*團迫浩方,中村孝志,仲一仁,野崎昭人,加藤宣之

第51回日本ウイルス学会学術集会・総会

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Event date： 2003.10.27 - 2003.10.29

Venue：京都市

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インターフェロン抵抗性C型肝炎ウイルスsubgenomic replicon細胞の樹立

難波克行,仲一仁,中村孝志,*團迫浩方,野崎昭人,白鳥康史,下遠野邦忠,加藤宣之

第51回日本ウイルス学会学術集会・総会

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Event date： 2003.10.27 - 2003.10.29

Venue：京都市

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ヒト培養細胞におけるC型肝炎ウイルスコア蛋白質のDNA修復能に及ぼす影響

*團迫浩方, 仲一仁,長沼篤,野崎昭人,加藤宣之

第62回日本癌学会総会

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Event date： 2003.9.25 - 2003.9.27

Venue：名古屋市

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C型肝炎ウイルス感染ヒト肝細胞由来の新規HCVサブジェノミックレプリコン細胞の樹立

加藤宣之,杉山和夫,*團迫浩方, 仲一仁,野崎昭人,下遠野邦忠

第62回日本癌学会総会

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Event date： 2003.9.25 - 2003.9.27

Venue：名古屋市

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インターフェロン刺激応答配列を介したC型肝炎ウイルスコア蛋白質の転写活性化能

*團迫浩方, 長沼篤,中村孝志,高見まり香,野崎昭人,加藤宣之

第25回日本分子生物学会年会

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Event date： 2002.11.11 - 2002.11.14

Venue：横浜市

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C型肝炎ウイルスコア蛋白質のインターフェロン刺激応答配列に対する転写活性化能

*團迫浩方, 長沼篤,中村孝志,高見まり香,野崎昭人,加藤宣之

第50回日本ウイルス学会学術集会・総会

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Event date： 2002.10.16 - 2002.10.18

Venue：札幌市

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C型肝炎ウイルスコア蛋白質のインターフェロンシグナル伝達経路の活性化

*團迫浩方, 長沼篤,野崎昭人,加藤宣之

第61回日本癌学会総会

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Event date： 2002.10.1 - 2002.10.3

Venue：東京

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C型肝炎ウイルスコア蛋白質のDNA修復系に及ぼす影響

* 團迫浩方, 長沼篤,中村孝志,野崎昭人,加藤宣之

第18回中国四国ウイルス研究会

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Event date： 2002.5.25 - 2002.5.26

Venue：岡山市

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C型肝炎ウイルスコア蛋白質によるインターフェロンシグナル伝達経路の活性化

長沼篤,*團迫浩方, 池田房雄,中村孝志,高木均,森昌朋,野崎昭人,加藤宣之

第24回日本分子生物学会年会

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Event date： 2001.12

Venue：横浜市

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C型肝炎ウイルスコア蛋白質のDNA修復系の及ぼす影響

*團迫浩方, 長沼篤,野崎昭人,中村孝志,高木均,森昌朋,加藤宣之

第24回日本分子生物学会年会

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Event date： 2001.12

Venue：横浜市

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Non-myeloablative stem cell transplant with fludarabine-based preparative regimen for advanced hematological malignancies.

Kozo Masuda, Katsuji Shinagawa, Kazuma Ikeda, Kinuyo Kaneda, Takayuki Fujiwara, * Dansako, Kensuke Kojima, Fumihiko Ishimaru and Mine Harada

30th Annual Meeting of the International Society for Experimental Hematology

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Event date： 2001.8

Venue：Tokyo, Japan

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Non-myeloablative stem cell transplantation with fludarabine based preparatory regimen in advanced hematologic malignancies.

Kozo Masuda, Katsuji Shinagawa, Kazuma Ikeda, Kinuyo Kaneda, Takayuki Fujiwara, *Hiromichi Dansako, Kensuke Kojima, Fumihiko Ishimaru and Mine Harada

第63回日本血液学会総会-アジア血液セッション-

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Event date： 2001.4

Venue：名古屋市

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先天性第?、第?因子合併欠乏症の遺伝子解析

*團迫浩方, 石丸文彦,高井豊,中瀬浩一,瀬崎伸夫,中山博之,竹中克斗,品川克至,池田和眞,新谷憲治,原田実根

第62回日本血液学会総会

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Event date： 2000.3

Venue：福岡市

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Awards

生物学研究奨励賞

2021.10 公益財団法人両備檉園記念財団

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岡山医学会賞・がん研究奨励賞（林原賞）

2016.6 岡山医学会

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学術奨励賞

2016.5 公益財団法人山陽放送学術文化財団

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Research Projects

分化制御NK細胞を用いた免疫療法による新規肝炎/肝癌治療の開発研究（分担）

2022 - 2024

国立研究開発法人日本医療研究開発機構肝炎等克服実用化研究事業肝炎等克服緊急対策研究事業

團迫浩方

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Authorship：Coinvestigator(s)

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効率的なHBV増殖細胞系、動物モデル系の確立とそれらを用いたHBV治療法開発（分担）

2021

国立研究開発法人日本医療研究開発機構肝炎等克服実用化研究事業Ｂ型肝炎創薬実用化等研究事業

團迫浩方

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Authorship：Coinvestigator(s)

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多機能幹細胞を用いた免疫賦活化療法による新規肝炎/肝癌治療の開発研究（分担）

2021

国立研究開発法人日本医療研究開発機構肝炎等克服実用化研究事業肝炎等克服緊急対策研究事業

團迫浩方

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Authorship：Coinvestigator(s)

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B型肝炎ウイルス感染に伴う細胞老化機構の分子生物学的解析

2021

公益財団法人両備檉園記念財団両備檉園記念財団研究助成

團迫浩方

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Authorship：Principal investigator

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B型肝炎ウイルス感染により細胞外に放出されるDAMPsの同定

2019

公益財団法人岡山医学振興会岡山医学振興会研究助成研究助成

團迫浩方

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Authorship：Principal investigator

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B型肝炎ウイルスの感染複製増殖機構解明による創薬基盤形成に関する研究（分担）

2017 - 2021

国立研究開発法人日本医療研究開発機構肝炎等克服実用化研究事業Ｂ型肝炎創薬実用化等研究事業

團迫浩方

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Research on the mechanism of hepatocarcinogenesis by long-term replication of hepatitis virus

Grant number：16H05196 2016.04 - 2019.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

Kato Nobuyuki, SEJIMA Hiroe, KOKU Irin, ONOMURA Daichi, HIRAMOTO Hiroki

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Grant amount：\17420000 （ Direct expense: \13400000 、 Indirect expense：\4020000 ）

We studied to clarify the relation of long-term replication of HBV or HCV and hepatocarcinogenesis, and then obtained the following results. (1) We clarified that VCX2 and AGR2 contributed to the expression control of the CPB2 gene whose expression level was remarkably decreased by the long-term HCV replication. (2) We successfully established two cloned cell lines exhibiting high HBV susceptibility by the subcloning of human immortalized hepatocyte NKNT-3 cells and human hepatoma Li23 cells. (3) We identified several genes whose expression levels significantly changed before and after HBV infection. (4) We developed the purification and quantitative methods for exosome produced from cultured cells. We demonstrated that the amount of exosome produced from HBV insusceptible cells was significantly higher than that of HBV susceptible cells.

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全長HCV RNA複製肝がん細胞株由来のエクソソーム内に存在するナチュラルキラー細胞を制御する機能性分子の同定

2016

ギリアド・サイエンシズ株式会社ギリアド・サイエンシズ株式会社研究助成研究助成

團迫浩方

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Authorship：Principal investigator

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B型肝炎ウイルスを認識する宿主因子cGASを特異的に亢進する薬剤の探索

2016

公益財団法人山陽放送学術文化財団山陽放送学術文化財団研究助成研究助成

團迫浩方

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Authorship：Principal investigator

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The mechanism of natural killer cell-mediated recognition against hepatitis virus infection.

Grant number：15K08498 2015.04 - 2018.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)

Dansako Hiromichi

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Authorship：Principal investigator

Grant amount：\4940000 （ Direct expense: \3800000 、 Indirect expense：\1140000 ）

In the present study, we demonstrated that hepatitis C virus (HCV) induced the cell surface expression of ULBP1 in human immortalized hepatocyte PH5CH8 cells and human hepatoma HuH-7 cell-derived RSc cells. Interestingly, NK cell line NK-92 induced cytotoxicity and interferon (IFN)-γ mRNA expression and subsequently reduced the levels of HCV RNA replication during the co-culture with HCV-infected RSc cells. We also suggested that NK-92 cells were stimulated by viral dsRNA relaesed from HCV-infected RSc cells and subsequently induced IFN-γ. From these results, we conclude that ULBP1 is a target of the NK cell-mediated innate immune response in HCV-infected human hepatocytes.

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B型肝炎ウイルス(HBV)の感染増殖を制御する分子機構の解明

2015

公益財団法人ウエスコ学術振興財団ウエスコ学術振興財団研究助成研究助成

團迫浩方

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Authorship：Principal investigator

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B型肝炎ウイルスによるインターフェロン-a抵抗性機構の解明

2014

公益財団法人両備檉園記念財団両備檉園記念財団研究助成研究助成

團迫浩方

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Authorship：Principal investigator

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Research on host factors which are involved in the progression of HCV-induced hepatic deseases

Grant number：25293110 2013.04 - 2016.03

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)

KATO NOBUYUKI, IKEDA Masanori, DANSAKO Hiromichi, SATOH Shinya

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Authorship：Coinvestigator(s)

Grant amount：\17030000 （ Direct expense: \13100000 、 Indirect expense：\3930000 ）

We studied to clarify the relation of long-term replication of HCV RNA and hepatocarcinogenesis, and then obtained the following results. (1) Regarding CPB2 and BASP1 genes, whose expression levels were remarkably suppressed by long-term replication of HCV RNA, we analyzed the molecular mechanism of the gene suppression, and partly elucidated it. (2) We found that miR-22 and miR-34a were significantly suppressed by long-term replication of HCV RNA. (3) We developed the reproducible and stable method that we could prepare exosome derived from human hepatocytes, and then we applied this method to the variability analysis of exosome in the long-term culture of HCV RNA-replicating cells. (4) Using immortalized human hepatocytes, we tried the development of a new model of HCV RNA-replicating cells, and then obtained the useful knowledge for future study.

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エクソソームを介したC型肝炎ウイルス感染・非感染細胞間自然免疫応答シグナル伝達機構の解明

2013

公益財団法人岡山医学振興会岡山医学振興会研究助成研究助成

團迫浩方

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Authorship：Principal investigator

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Development ofthe infection and proliferation systemof the 1bgenotype HCV based on the new idea

Grant number：24659207 2012

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research Grant-in-Aid for Challenging Exploratory Research

KATO Nobuyuki, DANSAKO Hiromichi, MORI Kyoko

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Authorship：Coinvestigator(s)

Grant amount：\3770000 （ Direct expense: \2900000 、 Indirect expense：\870000 ）

We tried the development of the infection and proliferation system of the 1b genotype hepatitis C virus (HCV) using human cultured cell lines. In this study, HCV-positive serum (1b genotype) was added tothe human hepatoma cell line Li23, which was found in 2009 as a cell line permitting the reproduction of the HCV RNA and human hepatoma cell line HuH-7 frequently used all over the world. We performed HCV infection under various conditions, however, we did not succeed to demonstrate an increase of the HCV. As one of the causes, we found that expression level of CLDN1, which was one of the HCV receptors, became very low, and thatthe supplement of CLDN1 expression was necessary.

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C型肝炎ウイルスに関する研究

2009

公益財団法人上原記念生命科学財団海外留学助成リサーチフェローシップ海外留学助成リサーチフェローシップ

團迫浩方

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Authorship：Principal investigator

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Disruption of innate immune response by hepatitis C virus

Grant number：19790340 2007 - 2008

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B) Grant-in-Aid for Young Scientists (B)

DANSAKO Hiromichi

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Grant amount：\3650000 （ Direct expense: \3200000 、 Indirect expense：\450000 ）

C型肝炎ウイルス(HCV)による自然免疫システムの撹乱機構の解明を目的として行った研究において、以下のような成果を得た。(1)従来の報告とは異なり、NS3-4A蛋白質はTRIF蛋白質を切断しておらず、自然免疫機構の抑制はRIG-I/IPS-1経路に限定されていた。(2)NS5B蛋白質によるIFN-βの産生はTLR3経路およびRIG-I/MDA5経路の活性化、さらには両経路の下流の転写因子IRF-3の活性化を経て誘導されていた。(3)NS5B蛋白質によるIFN-βの産生誘導はTRAF3やTRAF6のノックダウンにより亢進することを示した。また、活性化されたIRF-3の調節にTRAF6が関与している可能性が示唆された。(4)PH5CH8細胞において、NS5Bは二本鎖RNAを産生していることを示した。これらの研究成果により、HCVはこれら分子との相互作用により自然免疫システムを撹乱している可能性が示唆された。

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C型肝炎ウイルス治療薬の新規スクリーニングシステムの構築

Grant number：17790308 2005 - 2006

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B) Grant-in-Aid for Young Scientists (B)

團迫浩方

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Grant amount：\3400000 （ Direct expense: \3400000 ）

C型慢性肝炎患者に対する治癒率の改善のためには、現在のインターフェロン(IFN)とリバビリンの併用療法の改善だけでなく、IFNよりもC型肝炎ウイルス(HCV)の排除に効果的な薬剤をスクリーニングすることも重要である。そこで、従来のシステム(レポーター蛋白質の酵素活性を利用し、細胞内のHCV RNA複製レベルを定量、しかし生細胞のままの定量は困難)より簡便かつ安価に大規模な薬剤スクリーニングを遂行するために、緑色蛍光蛋白質であるEGFPをレポーター蛋白質に用いて、生細胞のままHCV RNA複製レベルの定量を可能にするシステムの開発を試みた。前年度までに、このシステムに必要なEGFP遺伝子を含む全長HCV RNA複製細胞を数クローン樹立することができ、細胞内でのHCV蛋白質およびEGFPの発現や、EGFPによる蛍光は観察できている。今年度は、この樹立した全長HCV RNA複製細胞を用いて、生細胞のままHCV RNA複製レベルを定量できるシステムの構築を行った。樹立できた数クローンのうち、OGN/C-5B/KE細胞(EGFP遺伝子をネオマイシン耐性遺伝子の前に、また適応変異としてNS3領域にK1609Eのアミノ酸置換を導入した全長HCV RNAが持続的に複製している細胞)のあるクローンが最も効率よく経時的に蛍光強度を上昇していることが確認できた。この細胞にIFN-αを処理し、その蛍光強度を生細胞のまま測定したところ、IFN-αの濃度依存的に減少していた。また、その値は、リアルタイムPCR法により定量したHCV RNA量と相関していた。また、ウエスタンブロット法により、EGFPやHCVコア蛋白質も減少していることが確認された。IFN-α以外に、HCVの排除に効果を示すことが報告されているIFN-β、IFN-γ、シクロスポリンAやフルバスタチンを同様に処理したところ、その蛍光強度とHCV RNA量の値は相関していた。また、IFN-αとシクロスポリンAあるいはIFN-αとフルバスタチンの併用処理は、それぞれの単剤処理に比べ、その蛍光強度は減少していた。これらの結果は、生細胞のままHCV RNAの複製レベルを定量することができるシステムを開発することができ、また、種々の薬剤処理も可能であることを示している(今研究実績は、現在、投稿準備中である)。

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