研究者詳細 - 鈴木　信弘

論文 - 鈴木　信弘

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Virome Analysis of Aphid Populations That Infest the Barley Field: The Discovery of Two Novel Groups of Nege/Kita-Like Viruses and Other Novel RNA Viruses 査読国際誌

Hideki Kondo, Miki Fujita, Hiroshi Hisano, Kiwamu Hyodo, Ida Bagus Andika, Nobuhiro Suzuki

Frontiers in Microbiology 11 509 2020年4月

記述言語：英語掲載種別：研究論文（学術雑誌）

Aphids (order Hemiptera) are important insect pests of crops and are also vectors of many plant viruses. However, little is known about aphid-infecting viruses, particularly their diversity and relationship to plant viruses. To investigate the aphid viromes, we performed deep sequencing analyses of the aphid transcriptomes from infested barley plants in a field in Japan. We discovered virus-like sequences related to nege/kita-, flavi-, tombus-, phenui-, mononega-, narna-, chryso-, partiti-, and luteoviruses. Using RT-PCR and sequence analyses, we determined almost complete sequences of seven nege/kitavirus-like virus genomes; one of which was a variant of the Wuhan house centipede virus (WHCV-1). The other six seem to belong to four novel viruses distantly related to Wuhan insect virus 9 (WhIV-9) or Hubei nege-like virus 4 (HVLV-4). We designated the four viruses as barley aphid RNA virus 1 to 4 (BARV-1 to -4). Moreover, some nege/kitavirus-like sequences were found by searches on the transcriptome shotgun assembly (TSA) libraries of arthropods and plants. Phylogenetic analyses showed that BARV-1 forms a clade with WHCV-1 and HVLV-4, whereas BARV-2 to -4 clustered with WhIV-9 and an aphid virus, Aphis glycines virus 3. Both virus groups (tentatively designated as Centivirus and Aphiglyvirus, respectively), together with arthropod virus-like TSAs, fill the phylogenetic gaps between the negeviruses and kitaviruses lineages. We also characterized the flavi/jingmen-like and tombus-like virus sequences as well as other RNA viruses, including six putative novel viruses, designated as barley aphid RNA viruses 5 to 10. Interestingly, we also discovered that some aphid-associated viruses, including nege/kita-like viruses, were present in different aphid species, raising a speculation that these viruses might be distributed across different aphid species with plants being the reservoirs. This study provides novel information on the diversity and spread of nege/kitavirus-related viruses and other RNA viruses that are associated with aphids.

DOI： 10.3389/fmicb.2020.00509

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Editorial: Frontiers in Fungal Virus Research 査読

Liying Sun, Nobuhiro Suzuki, Daohong Jiang, Massimo Turina, Jiatao Xie

Frontiers in Cellular and Infection Microbiology 9 456 2020年1月

Structure and assembly of double-stranded RNA mycoviruses 査読

Carlos P. Mata, Javier M. Rodríguez, Nobuhiro Suzuki, José R. Castón

Advances in Virus Research 108 213 - 247 2020年1月

記述言語：英語掲載種別：論文集(書籍)内論文

Mycoviruses are a diverse group that includes ssRNA, dsRNA, and ssDNA viruses, with or without a protein capsid, as well as with a complex envelope. Most mycoviruses are transmitted by cytoplasmic interchange and are thought to lack an extracellular phase in their infection cycle. Structural analysis has focused on dsRNA mycoviruses, which usually package their genome in a 120-subunit T = 1 icosahedral capsid, with a capsid protein (CP) dimer as the asymmetric unit. The atomic structure is available for four dsRNA mycovirus from different families: Saccharomyces cerevisiae virus L-A (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). Their capsids show structural variations of the same framework, with asymmetric or symmetric CP dimers respectively for ScV-L-A and PsV-F, dimers of similar domains of a single CP for PcV, or of two different proteins for RnQV1. The CP dimer is the building block, and assembly proceeds through dimers of dimers or pentamers of dimers, in which the genome is packed as ssRNA by interaction with CP and/or viral polymerase. These capsids remain structurally undisturbed throughout the viral cycle. The T = 1 capsid participates in RNA synthesis, organizing the viral polymerase (1–2 copies) and a single loosely packaged genome segment. It also acts as a molecular sieve, to allow the passage of viral transcripts and nucleotides, but to prevent triggering of host defense mechanisms. Due to the close mycovirus-host relationship, CP evolved to allocate peptide insertions with enzyme activity, as reflected in a rough outer capsid surface.

DOI： 10.1016/bs.aivir.2020.08.001

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Neo-virology: The raison d'etre of viruses 査読国際誌

Tokiko Watanabe, Nobuhiro Suzuki, Keizo Tomonaga, Hirofumi Sawa, Yoshiharu Matsuura, Yasushi Kawaguchi, Hideki Takahashi, Keizo Nagasaki, Yoshihiro Kawaoka

Virus Research 274 197751 - 197751 2019年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

Given that approximately 10 virus particles exist on Earth and all of them are parasitic in living organisms, it is not hard to imagine how virus infection might affect the physiology of hosts and their ecosystems. However, traditional virology research tends to focus on viral pathogenicity or the individual pathogenic viruses; hence, the significance of viruses and viral-mediated processes in the global ecosystem has been poorly understood. To identify the previously unrecognized “raison d'etre of viruses” in nature, we established a research community, designated as the ‘Neo-virology’ consortium. In this consortium, we define a virus as a component of the global ecosystem and our aim is to elucidate its key roles in host organisms, that is, the intra-host ecosystem. 31

DOI： 10.1016/j.virusres.2019.197751

ICTV Virus Taxonomy Profile: Megabirnaviridae. 査読国際誌

Sato, Y, Miyazaki, N, Kanematsu, S, Ghabrial, S. A, Hillman, B. I, Suzuki, N, ICTV Report, Consortium

Journal of General Virology 100 ( 9 ) 269 - 1270 2019年9月

Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae 査読

Shuhei Ohkita, Yui Lee, Quyet Nguyen, Kenichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

Virology 534 25 - 35 2019年8月

掲載種別：研究論文（学術雑誌）

Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5’ terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.

DOI： 10.1016/j.virol.2019.05.015

Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes) 査読

Yu Hsin Lin, Miki Fujita, Sotaro Chiba, Kiwamu Hyodo, Ida Bagus Andika, Nobuhiro Suzuki, Hideki Kondo

Virology 533 125 - 136 2019年7月

掲載種別：研究論文（学術雑誌）

There is still limited information on the diversity of (−)ssRNA viruses that infect fungi. Here, we have discovered two novel (−)ssRNA mycoviruses in the shiitake mushroom (Lentinula edodes). The first virus has a monopartite RNA genome and relates to that of mymonaviruses (Mononegavirales), especially to Hubei rhabdo-like virus 4 from arthropods and thus designated as Lentinula edodes negative-strand RNA virus 1. The second virus has a putative bipartite RNA genome and is related to the recently discovered bipartite or tripartite phenui-like viruses (Bunyavirales) associated with plants and ticks, and designated as Lentinula edodes negative-strand RNA virus 2 (LeNSRV2). LeNSRV2 is likely the first segmented (−)ssRNA virus known to infect fungi. Its smaller RNA segment encodes a putative nucleocapsid and a plant MP-like protein using a potential ambisense coding strategy. These findings enhance our understanding of the diversity, evolution and spread of (−)ssRNA viruses in fungi.

DOI： 10.1016/j.virol.2019.05.008

Isolation and characterization of a novel mycovirus infecting an edible mushroom, Grifola frondosa 査読

Akiko Komatsu, Hideki Kondo, Masayuki Sato, Atsushi Kurahashi, Kozo Nishibori, Nobuhiro Suzuki, Fumihiro Fujimori

Mycoscience 60 ( 4 ) 211 - 220 2019年7月

掲載種別：研究論文（学術雑誌）

Grifola frondosa (Maitake mushroom) is an important cultivated mushroom due to its medicinal and nutrient values. In this study, we isolated and characterized a novel partitivirus (named Grifola frondosa partitivirus 1, GfPV1) infecting a standard G. frondosa strain Gf-N2. This virus has a two-segmented dsRNA genome (dsRNA1 and dsRNA2) with nucleotide lengths of 2.3 and 2.2 kbp, respectively. The coding strand of dsRNA1 and dsRNA2 segments carries single open reading frame encoding RNA-dependent RNA polymerase (RdRp) and a coat protein (CP), respectively. BLAST searches and phylogenetic analyses showed that GfPV1 is most closely related to a betapartitivirus, Lentinula edodes partitivirus 1 (RdRp <70% and CP <60% amino acid sequence identities), but the sequence divergence suggests that GfPV1 is classifiable as a new member of the genus Betapartitivirus, family Partitiviridae. The presence of GfPV1 does not affect colony morphology and fruiting body development of G. frondosa. This is the first report investigating the effects of a mycovirus infection on the colony morphology and fruiting body development of G. frondosa. Interestingly, GfPV1 accumulations markedly decreased along with the fruiting body maturation stages, suggesting the inhibition of virus multiplication during sexual phase of the G. frondosa life cycle.

DOI： 10.1016/j.myc.2019.01.005

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A symptomless hypovirus, CHV4, facilitates stable infection of the chestnut blight fungus by a coinfecting reovirus likely through suppression of antiviral RNA silencing 査読

Annisa Aulia, Ida Bagus Andika, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

Virology 533 99 - 107 2019年7月

掲載種別：研究論文（学術雑誌）出版者・発行元：Elsevier {BV}

Field-collected US strain C18 of Cryphonectria parasitica, the chestnut blight fungus, was earlier reported to be infected by a double-stranded RNA virus, mycoreovirus 2 (MyRV2). Next-generation sequencing has revealed co-infection of C18 by a positive-strand RNA virus, hypovirus 4 (CHV4). The current molecular and genetic analyses showed interesting commensal interactions between the two viruses. CHV4 facilitated the stable infection and enhanced vertical transmission of MyRV2, which was readily lost during subculturing and showed reduced vertical transmission in single infections. Deletion of a key antiviral RNA silencing gene, dcl2, in isolate C18 increased stability of MyRV2 in single infections. The ability of CHV4 to facilitate stable infection with MyRV2 appears to be associated with the inhibitory effect of CHV4 on RNA silencing via compromising the induction of transcriptional up-regulation of dcl2. These results suggest that natural infection of isolate C18 by MyRV2 in the field was facilitated by CHV4 co-infection.

DOI： 10.1016/j.virol.2019.05.004

Novel victorivirus from a pakistani isolate of alternaria alternata lacking a typical translational stop/restart sequence signature 査読

Atif Jamal, Yukiyo Sato, Sabitree Shahi, Wajeeha Shamsi, Hideki Kondo, Nobuhiro Suzuki

Viruses 11 ( 6 ) 577 2019年6月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI} {AG

The family Totiviridae currently contains five genera Totivirus, Victorivirus, Leishmavirus, Trichomonasvirus, and Giardiavirus. Members in this family generally have a set of two-open reading frame (ORF) elements in their genome with the 5’-proximalORF (ORF1) encoding a capsid protein (CP) and the 3’-proximal one (ORF2) for RNA-dependent RNA polymerase (RdRp). How the downstream open reading frames (ORFs) are expressed is genus-specific. All victoriviruses characterized thus far appear to use the stop/restart translation mechanism, allowing for the expression of two separate protein products from bicitronic genome-sized viral mRNA, while the totiviruses use a -1 ribosomal frame-shifting that leads to a fusion product of CP and RdRp. We report the biological and molecular characterization of a novel victorivirus termed Alternaria alternata victorivirus 1 (AalVV1) isolated from Alternaria alternata in Pakistan. The phylogenetic and molecular analyses showed AalVV1 to be distinct from previously reported victoriviruses. AalVV1 appears to have a sequence signature required for the -1 frame-shifting at the ORF1/2 junction region, rather than a stop/restart key mediator. By contrast, SDS–polyacrylamide gel electrophoresis and peptide mass fingerprinting analyses of purified virion preparations suggested the expression of two protein products, not a CP-RdRp fusion product. How these proteins are expressed is discussed in this study. Possible effects of infection by this virus were tested in two fungal species: A. alternata and RNA silencing proficient and deficient strains of Cryphonectria parasitica, a model filamentous fungus. AalVV1 showed symptomless infection in all of these fungal strains, even in the RNA silencing deficient C. parasitica strain.

DOI： 10.3390/v11060577

Molecular and biological characterization of a novel botybirnavirus identified from a Pakistani isolate of Alternaria alternata 査読国際誌

Wajeeha Shamsi, Yukiyo Sato, Atif Jamal, Sabitree Shahi, Hideki Kondo, Nobuhiro Suzuki, Muhammad Faraz Bhatti

Virus Research 263 119 - 128 2019年4月

A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes. 査読

Kondo H, Chiba S, Maruyama K, Andika IB, Suzuki N

Virus research 262 37 - 47 2019年3月

"Integration of viral sequences into eukaryotic host genomes: legacy of ancient infections". 査読

Tomonaga K, Suzuki N, Berkhout B

Virus research 262 1 2019年3月

Hijacking a host scaffold protein, RACK1, for replication of a plant RNA virus. 査読

Hyodo K, Suzuki N, Okuno T

The New phytologist 221 ( 2 ) 935 - 945 2019年1月

Investigation of Host Range of and Host Defense against a Mitochondrially Replicating Mitovirus 査読

Sabitree Shahi, Ana Eusebio-Cope, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

Journal of Virology 93 ( 6 ) 2019年1月

The biological attributes, genome architecture and packaging of diverse multi-component fungal viruses 査読国際誌

Yukiyo Sato, Jos{\'{e, R Cast{\'{o } }n, Nobuhiro Suzuki

Current Opinion in Virology 33 55 - 65 2018年12月

ICTV Virus Taxonomy Profile: Quadriviridae. 査読

Chiba S, Castón JR, Ghabrial SA, Suzuki N, Ictv Report, Consortium

The Journal of general virology 99 ( 11 ) 1480 - 1481 2018年11月

Novel Mitoviruses and a Unique Tymo-Like Virus in Hypovirulent and Virulent Strains of the Fusarium Head Blight Fungus, Fusarium boothii 査読

Mizutani Y, Abraham A, Uesaka K, Kondo H, Suga H, Suzuki N, Chiba S

Viruses 10 ( 11 ) 2018年10月

Capsid Structure of dsRNA Fungal Viruses. 査読

Luque D, Mata CP, Suzuki N, Ghabrial SA, Castón JR

Viruses 10 ( 9 ) 2018年9月

ICTV virus taxonomy profile: Hypoviridae 査読

Nobuhiro Suzuki, Said A. Ghabrial, Kook-Hyung Kim, Michael Pearson, Shin-Yi L. Marzano, Hajime Yaegashi, Jiatao Xie, Lihua Guo, Hideki Kondo, Igor Koloniuk, Bradley I. Hillman, Elliot J. Lefkowitz, Andrew J. Davison, Stuart G. Siddell, Sead Sabanadzovic, Donald B. Smith, Richard J. Orton, Peter Simmonds, ICTV Report Consortium

Journal of General Virology 99 ( 5 ) 615 - 616 2018年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Microbiology Society

The Hypoviridae, comprising one genus, Hypovirus, is a family of capsidless viruses with positive-sense, ssRNA genomes of 9.1–12.7 kb that possess either a single large ORF or two ORFs. The ORFs appear to be translated from genomic RNA by non-canonical mechanisms, i.e. internal ribosome entry site-mediated and stop/restart translation. Hypoviruses have been detected in ascomycetous or basidiomycetous filamentous fungi, and are considered to be replicated in host Golgi-derived, lipid vesicles that contain their dsRNA as a replicative form. Some hypoviruses induce hypovirulence to host fungi, while others do not. This is a summary of the current ICTV report on the taxonomy of the Hypoviridae, which is available at www.ictv.global/report/hypoviridae.

DOI： 10.1099/jgv.0.001055

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論文 - 鈴木　信弘