研究者詳細 - 佐々木　崇了

A purification system for 64Cu produced by a biomedical cyclotron for antibody PET imaging 査読

Teruaki Toyota, Tadashi Hanafusa, Takashi Oda, Iwane Koumura, Takanori Sasaki, Eiji Matsuura, Hiromi Kumon, Tsuneo Yano, Toshiro Ono

Journal of Radioanalytical and Nuclear Chemistry 298 ( 1 ) 295 - 300 2013年

記述言語：英語掲載種別：研究論文（学術雑誌）

Ion exchange is a simple and efficient method for separating no-carrier-added 64Cu from an irradiated Ni target. We developed a semi-automated two-round 64Cu separation system equipped with a strong-base anion exchange resin column. We first verified the efficiency of the system using a non-radioactive substitute consisting of 25 mg of Ni and 127 ng of Cu, and confirmed that Cu was completely eluted at the second round of the separation step. After the bombardment, separation of 64Cu from the Ni target was achieved with high radiochemical purity. 64Cu produced and separated in this study had an extremely low level of Ni impurity. It could be used for labeling monoclonal antibodies for antibody positron emission tomography imaging and synthesizing radiopharmaceuticals. © 2012 The Author(s).

DOI： 10.1007/s10967-012-2340-7

Scopus

岡山大学におけるPET研究施設の治験薬GMP（Good Manufacturing Practice）体制招待

佐々木崇了, 松浦栄次, 公文裕巳, 矢野恒夫

Pharm Tech Japan 29 123 - 128 2013年

In Vitro Assessment of Factors Affecting the Apparent Diffusion Coefficient of Ramos Cells Using Bio-phantoms 査読

Takanori Sasaki, Masahiro Kuroda, Kazunori Katashima, Masakazu Ashida, Hidenobu Matsuzaki, Junichi Asaumi, Jun Murakami, Seiichiro Ohno, Hirokazu Kato, Susumu Kanazawa

ACTA MEDICA OKAYAMA 66 ( 3 ) 263 - 270 2012年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OKAYAMA UNIV MED SCHOOL

The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkitt's lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 gm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in vitro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values.

DOI： 10.18926/AMO/48566

Administration of warmed intravenous fluids for medetomidine-induced hypothermia in normal dogs 査読

Kanda T, Ikeda M, Ouchi M, Nagasaki A, Yamamoto R, Morishita T, Maeta N, Sasaki T, Furumoto K, Kake S, Murao N, Furukawa

Japanese Journal of Veterinary Anesthesia and Surgery 42 ( 1 ) 7 - 12 2011年10月

小動物臨床における3Dプリンターの応用査読

神田鉄平, 佐々木崇了, 前田憲孝, 藤岡透, 長井新, 古本佳代, 古川敏紀

日本獣医師会雑誌 64 ( 8 ) 640 - 643 2011年8月

外側鼓室胞骨切り術に超音波手術器を使用した慢性化膿性中耳炎のアメリカン・コッカー・スパニエルの1例査読

前田憲孝, 佐々木崇了, 神田鉄平, 藤岡透, 古川敏紀

獣医麻酔外科学雑誌 42 ( 2 ) 21 - 24 2011年

耳鏡を用いたラットの性周期判定査読

古本佳代, 神田鉄平, 前田憲孝, 佐々木崇了, 村尾信義, 古川敏紀

実験動物技術 46 ( 2 ) 2011年

Spinal epidural empyema in a cat 査読

Noritaka Maeta, Teppei Kanda, Takanori Sasaki, Takehito Morita, Toshinori Furukawa

JOURNAL OF FELINE MEDICINE AND SURGERY 12 ( 6 ) 494 - 497 2010年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI LTD

The diagnosis and surgical treatment of spinal epidural empyema (SEE) in a 2-year-old neutered male domestic shorthaired cat is described. SEE was diagnosed by computed tomographic myelography (CT myelography) and surgical exploration. The lesion was missed on both non-enhanced CT and conventional myelography. SEE should be considered in the differential diagnosis of progressive myelopathy in cats, and CT myelography should be undertaken when magnetic resonance imaging (MRI) cannot be performed. (C) 2010 ISFM and AAFP. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jfms.2010.01.015

A new phantom using polyethylene glycol as an apparent diffusion coefficient standard for MR imaging 査読

Ryohei Matsuya, Masahiro Kuroda, Yoshitsugu Matsumoto, Hirokazu Kato, Hidenobu Matsuzaki, Junichi Asaumi, Jun Murakami, Kazunori Katashima, Masakazu Ashida, Takanori Sasaki, Tetsuro Sei, Kengo Himei, Kuniaki Katsui, Norihisa Katayama, Mitsuhiro Takemoto, Susumu Kanazawa, Seiichi Mimura, Seiichiro Oono, Takuichi Kitayama, Seiji Tahara, Keiji Inamura

INTERNATIONAL JOURNAL OF ONCOLOGY 35 ( 4 ) 893 - 900 2009年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPANDIDOS PUBL LTD

In recent years, magnetic resonance imaging (MRI) with diffusion-weighted imaging (DWI) has seen wide clinical use, such as for early detection of cerebrovascular diseases and whole body screening for tumors. The apparent diffusion coefficient (ADC) standard phantom, which mimics the ADC values of several lesions in the body, is indispensable for the development of new pulse sequences for DWI, such as diffusion-weighted whole-body imaging with background body-signal suppression (DWIBS). However, information on the ADC values of the previously reported ADC standard phantoms is limited, because these phantoms were made using only a few different materials at a limited range of concentrations, and the ADC values were measured only at certain temperatures. It has been considered difficult, if not impossible, to create a phantom that provides arbitrary ADC values, because it is difficult to calculate the concentrations of the materials and the temperature at ADC measurement. In this study, we used polyethylene glycol (PEG) as a phantom material, and developed an empirical formula to calculate the PEG concentration at any measurement temperature to obtain arbitrary ADC values of the phantom. DWI images of phantoms made using seven different PEG concentrations were taken under heating from 17 to 46 degrees C at 1 degrees C intervals. Using ADC values calculated from these DWI images, we developed two empirical formulas: i) an empirical formula to calculate the ADC values of phantoms made using any PEG concentration at any measurement temperature; and ii) an empirical formula to calculate PEG concentrations to obtain arbitrary ADC values at any measurement temperature. We inspected the accuracy of these empirical formulas by newly made PEG phantoms. A comparison between the ADC values calculated with the empirical formulas and the measured ADC values confirmed the high accuracy of these formulas. PEG phantoms are safe, inexpensive and easy to make, compared with the previously reported ADC standard phantoms. Our empirical formulas enable us to calculate PEG concentrations that provide arbitrary ADC values at any measurement temperature. The empirical formulas could be used within a range of ADC values from 0.37x10(-3) to 3.67x10(-3) mm(2)/s, PEG concentrations from 0 to 120 mM, and measurement temperatures from IS to 45 degrees C. Using these formulas, it would be possible to make standard phantoms that mimic the ADC values of any clinical lesions. The PEG phantom might thus be an excellent new ADC standard phantom for MRI with DWI.

DOI： 10.3892/ijo_00000404

In vitro experimental study of the relationship between the apparent diffusion coefficient and changes in cellularity and cell morphology 査読

Yoshitsugu Matsumoto, Masahiro Kuroda, Ryohei Matsuya, Hirokazu Kato, Koichi Shibuya, Masataka Oita, Atsushi Kawabe, Hidenobu Matsuzaki, Junichi Asaumi, Jun Murakami, Kazunori Katashima, Masakazu Ashida, Takanor Sasaki, Tetsuro Sei, Susumu Kanazawa, Seiichi Mimura, Seiichiro Oono, Takuichi Kitayama, Seiji Tahara, Keiji Inamura

ONCOLOGY REPORTS 22 ( 3 ) 641 - 648 2009年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPANDIDOS PUBL LTD

Diffusion-weighted magnetic resonance imaging (MRI) is frequently used clinically, and is available for the whole-body screening for tumors. The exact mechanism by which the apparent diffusion coefficient (ADC) value decreases in tumorous tissue remains unclear, although various theories have been proposed, including intracellular and extracellular factor theories. It is impossible to distinguish each factor in the intracellular and extracellular spaces as the source of MR signal generation by means of conventional comparison between MR images and pathological specimens. Other factors which have been reported to affect ADC include cellularity and cellular edema of human tissues, and temperature of phantoms at the time of measurement. We employed a new technique that enables cellular MR imaging using a newly developed bio-phantom containing a living culture tumor cell line, Jurkat-N1. We investigated possible reasons for observed decreases in ADC values for tumors, and we considered the contribution of both the intracellular and extracellular space to such a decrease. The ADC values of the bio-phantom increased with increasing heat exposure from 27 to 45 degrees C. ADC values also increased after the destruction by sonication of tumor cell membranes. ADC values decreased as cellularity increased in the bio-phantorn. ADC values decreased due to cellular edema caused by a low salt concentration in the bio-phantom. Changes in pressure in the bio-phantom had no effect on the observed ADC values. We calculated both the intracellular ADC and extracellular ADC values using the ADC values, cellularity, and cellular volume of Jurkat-N1 cells in the bio-phantom. The extracellular ADC values in the bio-phantom were estimated to be lower than the ADC value of distilled water. These results indicate that not only intracellular ADC values, but also extracellular ADC values contribute to the determination of the ADC values of bio-phantoms. This is the first report to have examined the contribution of intracellular and extracellular space on the ADC values of bio-phantoms containing cultured tumor cells.

DOI： 10.3892/or_00000484