Faculty Profiles - HAYAKAWA Tohru

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Development of efficient system to improve mosquitocidal toxins of soil bacteria

Annual report of the Okayama foundation for science and technology 32 40 - 42 2023.10

環境に優しく持続利用可能な微生物殺蚊剤を開発するための分子基盤

早川徹

研究レポート集2022, 公益財団法人八雲環境科学振興財団 23 104 - 109 2022.11

蚊を殺すトキシン由来のポリペプチドを利用して効率的なタンパク質の生産系を構築する

早川徹

昆虫と自然 51 ( 7 ) 41 - 42 2016

Bacillus thuringiensis TK-E6が産生する非3-ドメイン型Cryタンパク質の殺ボウフラ活性

根来綾子, 江草佳務, 上林智仁, 早崎君江, 岡本成平, 東慶直, 東慶直, 早川徹, 武部聡, 武部聡

日本農芸化学会大会講演要旨集(Web) 2015 2015

Bacillus thuringiensis由来のタンパク質結晶化因子4AaCterタグを用いた殺虫タンパク質の生産

杉森勇太, 佐藤一樹, 石田達彦, 早川徹, 酒井裕, 東慶直, 武部聡

日本農芸化学会大会講演要旨集 2011 2011

細胞損傷蛋白質パラスポリンを通して見るCryタンパク質の可能性

早川徹, 酒井裕

蚕糸昆虫バイオテック (SANSHI-KONCHU BIOTEC) 77 ( 3 ) 205 - 210 2008

Expression of Bombyx mori Aminopeptidase N on Plasma Membrane of Cultured Insect Cells and Analysis of Its Receptor Function for Insecticidal Cry Toxins

Noguchi Rieko, Ishikawa Toshiki, Haginoya Kohsuke, Shitomi Yasuyuki, Sato Ryoichi, Hayakawa Tohru, Hori Hidetaka

Bulletin of the Faculty of Agriculture, Niigata University 第60巻 ( 1号 ) 73 - 81 2007

Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (bulletin of university, research institution) Publisher：Niigata University

Aminopeptidase N(APN) localizing on the apical membrane of midgut epithelial cells is one of the plausible candidate of the receptor proteins for Bacillus thuringiensis Cry1Aa toxin which specifically kills lepidopteran insects. However, comprehensive evidences to address above possibility have not been established yet. We tried to express the APN1, BmAPN1, onto plasma membrane of cultured insect cells to investigate the interaction with Cry1Aa. Gene expression system was constructed with High five cultured insect cells and baculovirus expression system. On 48 h after the infection of the cells with gene modified baculovirus, 110 kDa BmAPN1 was expressed on the cell membranes. Aminopeptidase activity was ten times higher than that of non-infected cultured cells. The recombinant BmAPN1 was the same size as native one localizing on the brush border membrane of midgut epithelial cells. The transformed cells expressing BmAPN1 is a good system for the investigation of interaction between APN and Cry1Aa toxin.

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Other Link： http://hdl.handle.net/10191/5041

Quantification of Aluminum Content in the Compost Made with Sludge Wasted from a Bean Curd Factory

Noguchi Rieko, Mohamad Monjil S., Kohya Hiroe, Miura Mizuho, Ono Hironori, Takahashi Hitomi, Haginoya Kohsuke, Hayakawa Tohru, Hori Hidetaka

Bulletin of the Faculty of Agriculture, Niigata University 60 ( 1 ) 47 - 51 2007

Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (bulletin of university, research institution) Publisher：Niigata University

Wasted sludge from a bean curd factory was mixed with newspaper chips cut (12 mm ×3 mm) and aerated right after the placement of the mixture into a fermenter. The sludge was mixed with newspaper chips at 15% (w/w) and composted under continuous aeration at 80 L/min/m2 bottom area of the fermenter. Hereafter we called the compost as paper-compost. The resulted paper-compost showed no inhibitory effect in germination of the komatsuna, Brassica nappas seeds and increased thereafter growth of them. On the other hand, the application of chemical fertilizer at the same amount of nitrogen content as that of paper-compost showed almost no growth of the plantlets. Generally, polyaluminum chlorides were added to the wasted water in active sludge sewer system to coagulate the excess amount of sludge, therefore, there is a possibility that the Al to remain after even the completion of composting, then the aluminum contents in the compost was evaluated with an atomic absorption spectrophotometer. The content in the compost was 160 mg/kg compost dried. On the other hand, the aluminum contents in wasted sludge and newspaper which were used to make the compost were 110 and 150 mg/kg, respectively. Thus, the aluminum in the composts was thought to be derived from those newspapers and active sludge. At least, polyaluminum chloride used to coagulate the excess sludge was not seemed to remain in the compost.

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Other Link： http://agriknowledge.affrc.go.jp/RN/2010742738

Loading of Fura-2 into Liquid Organ Cultured Adventitious Root of Turnip (Brassica rapa L.) Resistant to Clubroot Pathogen Plasmodiophora brassicae and Determination of [Ca2+]cyt

Takahashi Hideyuki, Ishikawa Toshiki, Hayakawa Tohru, Itoh Kimiko, Mitsui Toshiaki, Hori Hidetaka

Bulletin of the Faculty of Agriculture, Niigata University 60 ( 1 ) 61 - 66 2007

Language：English Publishing type：Rapid communication, short report, research note, etc. (bulletin of university, research institution) Publisher：Niigata University

Our previous study using liquid organ cultured adventitious roots from turnip (Brassica rapa L.) showed that Ca2+ is required for induction of defense responses in the cultured roots on the treatment with Plasmodiophora brassicae resting spores (Takahashi et al., 2006). To evaluate change in [Ca2+]cyt in cultured root cells on contact with the spores, acetoxymethyl ester derivative of Fura-2 (Fura-2/AM) was loaded into the roots. When ionophore A23187 was treated with Ca2+ simultaneously, the Fura-2 fluorescence ratio that represents relative [Ca2+]cyt increased promptly, showing that the Fura-2/AM system is suitable to evaluate [Ca2+]cyt change in cultured root cells in a second range. Applicability of this method for studies on Ca2+ fluctuation against various extracellular stimuli was supported by observations that treatment with mannitol or NaCl also immediately increased the Fura-2 ratio. When the Fura-2/AM loaded roots were treated with resting spores, no [Ca2+]cyt change was observed during 500 second but when treated with spores pre-incubated with germination-enhancing suspension (GES), a slight but reproducible increase in [Ca2+]cyt was observed. We conclude that although further analysis is needed, the Fura-2/AM system will contribute to revealing the Ca2+ involvement in clubroot-resistance response.

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Other Link： http://agriknowledge.affrc.go.jp/RN/2010742740

The mechanism of resistance to Cry1A toxins analysis of the interaction between Cry1A and brush border membrane and peritrophic membrane of Bombyx mori. Reviewed

Hayakawa T, Hossain DM, Shitomi Y, Sato R, Hori H

Proceedings international conference on Biopesticides 4, Chiang Mai, Thailand. (Uruyakorn Chansang, Duangkhae Sitthicharoenchai, Mir S. Mulla eds) 81 - 89 2006

Detection of membrane-inserted region of Cry1Aa of Bacullus thuringiensis Reviewed

Tomimoto K, Hayakawa T, Mitsui T, Hori H

Biotechnology of Bacillus thuringiensis (Ngo Dinh Binh, Ray J. Akhurst, Donald H. Dean eds) 5 119 - 124 2005

Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis

HAGINOYA Kohsuke, KATO Taisuke, HIGUCHI Masahiro, SHITOMI Yasuyuki, ASAKURA Tsuyoshi, HAYAKAWA Tohru, MITSUI Toshiaki, HORI Hidetaka

Bulletin of the Faculty of Agriculture, Niigata University 57 ( 2 ) 115 - 120 2005

Allergenic assessment of Cry1Aa using Rat digestive juice and mast cells. Reviewed

Egawa Y, Okuyama K, Hara T, Tomimoto K, Hayakawa T, Hori H

Biotechnology of Bacillus thuringiensis(Ngo Dinh Binh, Ray J. Akhurst, Donald H. Dean eds) 5 389 - 394 2005

A novel 252kDa protein from BBMV of Bombyx mori, which bound with Cry1A toxins but did not bind with anti-APN or BtR175 antibodies. Reviewed

Hossain DM, Shitomi Y, Moriyama K, Higuchi M, Hayakawa T, Sato R, Hori H

Biotechnology of Bacillus thuringiensis (Ngo Dinh Binh, Ray J. Akhurst, Donald H. Dean eds) 5 223 - 228 2005

Characterization of Bombyx mori AP96 which binds with Cry1Ac. Reviewed

Shitomi Y, Moriyama K, Hossain DM, Higuchi M, Hayakawa T, Miyamoto K, Mitsui T, Nakanishi K, Sato R, Hori H

Biotechnology of Bacillus thuringiensis (Ngo Dinh Binh, Ray J. Akhurst, Donald H. Dean eds) 5 187 - 194 2005

Proteomic approach for comparative analysis of midgut proteins from Plutella xylostella that is highly resistant to Cry1Ac toxin. Reviewed

Ahmad M, Maruyama T, Higuchi M, Hayakawa T, Mitsui T, Sato R, Hori H

Biotechnology of Bacillus thuringiensis (Ngo Dinh Binh, Ray J. Akhurst, Donald H. Dean eds) 5 177 - 182 2005

Novel binding proteins for Cry1A of Bacillus thuringiensis toxins and complicated killing mechanism Reviewed

Hayakawa T, Shitomi Y, Hossain DM, Higuchi M, Sato R, Maruyama T, Miyamoto K, Hori H

Biotechnology of Bacillus thuringiensis (Ngo Dinh Binh, Ray J. Akhurst, Donald H. Dean eds) 5 163 - 176 2005

Differential Extraction of the Proteins Localizing on Cell Membranes of Midgut from Bombyx mori with Triton X-114

Bulletin of the Faculty of Agriculture,Niigata University 55 ( 2 ) 133 - 141 2003

Activity of Phosphatidylinositol/UDP-GlcNAc:GlcNAc Transferase in Midgut Tissue of Bombyx mori

Bulletin of the Faculty of Agriculture,Niigata University 55 ( 2 ) 123 - 131 2003

Enhancement of Germination of Spores from Obligatory Plant Pathogen,Plasmodiophora brassicae Causing Clubroot Disease

OGAWA Satoshi, TAKAHASHI Hideyuki, HAYAKAWA Tohru

Bulletin of the Faculty of Agriculture, Niigata University 54 ( 1 ) 35 - 43 2001.8

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