Faculty Profiles - OKAMOTO Kuniaki

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破骨細胞分化を制御する新規Rabタンパク質の同定と機能解析

山口優, 坂井詠子, 岡元邦彰, 鍛治屋浩, 岡部幸司, 筑波隆幸

Journal of Oral Biosciences Supplement (Web) 2017 2017

tert-ButylhydroquinoneによるIrf8とMafBの発現増加を介した破骨細胞分化抑制機構

坂井詠子, 山口優, 福間裕, 西下一久, 岡元邦彰, 筑波隆幸

日本生化学会大会(Web) 89th 2016

Keap1/Nrf2の破骨細胞と骨芽細胞分化における役割

坂井詠子, 福間裕, 山口優, 西下一久, 岡元邦彰, 筑波隆幸

日本生化学会大会(Web) 88th 2015

歯科用モノマーによる破骨細胞分化に対する効果

稲光宏之, 岡元邦彰, 坂井詠子, 村田比呂司, 筑波隆幸

Journal of Oral Biosciences Supplement 2014 180 - 180 2014.9

Chemical constituent of Sanguisorba officinalis inhibits osteoclastogenesis

Eiko Sakai, Mayumi Iwatake, Yutaka Fukuma, Kazuhisa Nishishita, Kuniaki Okamoto, Takashi Tanaka, Takayuki Tsukuba

JOURNAL OF PHARMACOLOGICAL SCIENCES 124 232P - 232P 2014

コバルトプロトポルフィリンの破骨細胞分化および活性化に対する影響

八島由佳, 八島由佳, 岡元邦彰, 坂井詠子, 西下一久, 筑波隆幸

Journal of Oral Biosciences Supplement (Web) 2014 ROMBUNNO.P2‐33 (WEB ONLY) 2014

Nrf2遺伝子欠損マウスにおける酸化ストレスと骨代謝

坂井詠子, 山口優, 福間裕, 菅原めぐみ, 菅原めぐみ, 西下一久, 岡元邦彰, 筑波隆幸

日本生化学会大会(Web) 87th 2014

Up-regulation mechanisms of endosomal/lysosomal proteins during osteoblast differentiation

Erika Yoneshima, Kuniaki Okamoto, Eiko Sakai, Noriaki Yoshida, Takayuki Tsukuba

JOURNAL OF PHARMACOLOGICAL SCIENCES 121 170P - 170P 2013

Cafestol prevents osteoclastogenesis via impairment of NFATc1 expression and blocking of Erk phosphorylation

Yutaka Fukuma, Eiko Sakai, Megumi Sugawara, Erika Yoneshima, Kazuhisa Nishishita, Kuniaki Okamoto, Takayuki Tsukuba

JOURNAL OF PHARMACOLOGICAL SCIENCES 121 249P - 249P 2013

Identification of two transcrips and in vivo promoter analysis for cathepsin E

Kuniaki Okamoto, Yoshiko Okamoto, Eiko Sakai, Kazuhisa Nishishita, Kenji Yamamoto, Takayuki Tstikuba

JOURNAL OF PHARMACOLOGICAL SCIENCES 118 259P - 259P 2012

Accumulation of NADPH oxidase (NOX2) and increased oxidative stress in cathepsin E-deficient macrophages

Takayuki Tsukuba, Tomoko Kadowaki, Kuniaki Okamoto, Kenji Yamamoto

JOURNAL OF PHARMACOLOGICAL SCIENCES 118 256P - 256P 2012

Fisetinl prevents osteoclastogenesis via impairment of NFATc1 expression and blocking of Erk phosphorylation

Megumi Sugawara, Eiko Sakai, Kazuhisa Nishishita, Yutaka Fukuma, Kuniaki Okamoto, Noriaki Yoshida, Takayuki Tsukuba

JOURNAL OF PHARMACOLOGICAL SCIENCES 118 164P - 164P 2012

Receptor activator of NF-kappa B ligand dependent heme oxygenase-1 suppression is required for high mobility group box 1 release, caspase-3 activation, and subsequent osteoclastogenesis

Eiko Sakai, Megumi Shimada, Kazuhisa Nishishita, Yutaka Fukuma, Kuniaki Okamoto, Takayuki Tsukuba

JOURNAL OF PHARMACOLOGICAL SCIENCES 115 219P - 219P 2011

Nrf2 activators inhibit osteoclastogenesis induced by receptor activator of NF-kappa B ligand

Reiko Fumimoto, Hiroshi Sakamoto, Yu Yamaguchi, Eiko Sakai, Kuniaki Okamoto, Takayuki Tsukuba

JOURNAL OF PHARMACOLOGICAL SCIENCES 115 219P - 219P 2011

tert-Butylhydroquinoneの破骨細胞形成と骨吸収活性への影響

山口優, 坂井詠子, 岡元邦彰, 筑波隆幸

Journal of Oral Biosciences 53 ( Supplement ) 2011

Transcription factor Sp1 regulates the expression of the murine cathepsin E gene in gastric adenosarcoma cells

Kuniaki Okamoto, Yoshiko Okamoto, Tomoyo Kawakubo, Kenji Yamamoto, Takayuki Tsukuba

JOURNAL OF PHARMACOLOGICAL SCIENCES 112 216P - 216P 2010

Autophagy impairment associated with increased aberrant mitochondria and oxidative stress in cathepsin E-deficient macrophages

Takayuki Tsukuba, Michiyo Yanagawa, Tomoko Kadowaki, Yoshiko Okamoto, Kuniaki Okamoto, Kenji Yamamoto

JOURNAL OF PHARMACOLOGICAL SCIENCES 112 216P - 216P 2010

カテプシンE欠損における破骨細胞分化への影響

岡元邦彰, 坂井詠子, 西下一久, 福間裕, 坂元裕, 文元玲子, 山口優, 山本健二, 筑波隆幸

生化学 2010

Carbon dioxide laser irradiation stimulates mineralization in rat dental pulp cells

Y. Yasuda, E. Ohtomo, T. Tsukuba, K. Okamoto, T. Saito

INTERNATIONAL ENDODONTIC JOURNAL 42 ( 10 ) 940 - 946 2009.10

Language：English Publisher：WILEY-BLACKWELL PUBLISHING, INC

P>Aim
To examine the effect of carbon dioxide laser irradiation on mineralization in dental pulp cells.
Methodology
Rat dental pulp cells were irradiated with a carbon dioxide laser at 2 W output power for 20, 40 and 60 s, and were cultured in ascorbic acid and beta-glycerophosphate containing media. Cell viability was examined 24 h after laser irradiation by a modified MTT assay. Alizarin Red S staining was performed 10 days after laser irradiation. The amounts of secreted collagen from the cells after irradiation were quantified following Sirius Red staining. The expression levels of collagen type I and HSP47, collagen-binding stress protein, were analysed by real-time PCR. HSP47 protein expression was examined by Western blotting. Statistical analysis was performed using one-way analysis of variance (anova) followed by the Tukey's multiple comparison test.
Results
The cell viability was not affected by laser irradiation at 2 W for up to 40 s. However, it was significantly decreased by 20% at 60 s (P < 0.05). The amount of mineralization after 10 days of irradiation at 2 W for 40 s was significantly increased in comparison to the other conditions (P < 0.05). The extracellular collagen production was significantly increased by 73% on day 2 and 38% on day 4 after laser irradiation (P < 0.05). Although collagen type I gene expression was not changed by laser irradiation, HSP47 gene and protein expression was induced within 12 and 24 h, respectively.
Conclusions
These results suggested that carbon dioxide laser irradiation stimulated mineralization in dental pulp cells. The laser irradiation also increased HSP47 expression but not collagen gene expression.

DOI： 10.1111/j.1365-2591.2009.01598.x

Web of Science

researchmap

Impaired chemotaxis and cell adhesion due to decrease in several cell-surface receptors in cathepsin E-deficient macrophages

Takayuki Tsukuba, Michiyo Yanagawa, Kuniaki Okamoto, Yoshiko Okamoto, Yoshiyuki Yasuda, Keiichi I. Nakayama, Tomoko Kadowaki, Kenji Yamamoto

JOURNAL OF BIOCHEMISTRY 145 ( 5 ) 565 - 573 2009.5

Language：English Publisher：OXFORD UNIV PRESS

Cathepsin E is an endo-lysosomal aspartic proteinase exclusively present in immune system cells. Previous studies have shown that cathepsin E-deficient (CatE(/)) mice display aberrant immune responses such as atopic dermatitis and higher susceptibility to bacterial infection. However, the mechanisms underlying abnormal immune responses induced by cathepsin E deficiency are still unclear. In this study, we found that the cell-surface levels of chemotactic receptors, including chemokine receptor (CCR)-2 and N-formyl peptide receptors (FPRs), were clearly diminished in CatE(/)macrophages compared with those in wild-type cells. Consistently, chemotaxis of CatE(/)macrophages to MCP-1 and N-formyl-methionyl-leucyl-phenylalanine was also decreased. Similar to the chemotactic receptors, the surface expressions of the adhesion receptors CD18 (integrin (2)) and CD 29 (integrin (1)) in CatE(/) macrophages were significantly decreased, thereby reducing cell attachment of CatE(/) macrophages. These results indicate that the defects in chemotaxis and cell adhesion are likely to be involved in the imperfect function of CatE(/)macrophages.

DOI： 10.1093/jb/mvp016

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