Research Projects -
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メタゲノム解析によるCOPD合併肺がんの臓器横断的ディスバイオーシスの解明
Grant number：23K18317 2023.06 - 2026.03
日本学術振興会 科学研究費助成事業 挑戦的研究(萌芽)
枝園 和彦, 冨田 秀太, 遠西 大輔, 豊岡 伸一, 山本 寛斉, 諏澤 憲, 山中 玲子
Grant amount：\6370000 （ Direct expense: \4900000 、 Indirect expense：\1470000 ）
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歯周治療による大腸がん重症化予防の試み～F. nucleatum血流感染リスク低減を介して～
Grant number：23K09502 2023.04 - 2027.03
日本学術振興会 科学研究費助成事業 基盤研究(C)
山中 玲子, 森田 学, 江國 大輔, 寺石 文則, 横井 彩
Grant amount：\4680000 （ Direct expense: \3600000 、 Indirect expense：\1080000 ）
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周術期ガム咀嚼トレーニングは食道がん術後のフレイルの進行を予防するか
Grant number：22-3-11 2022.10 - 2023.03
公益財団法人８０２０推進財団 令和４年度 ８０２０研究事業
横井彩、江國大輔、森田学
Authorship：Principal investigator Grant type：Competitive
Grant amount：\600000 （ Direct expense: \600000 ）
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摂食嚥下療法は頭頸部がんサバイバーの復職支援となりうるか？
Grant number：21K10209 2021.04 - 2024.03
日本学術振興会 科学研究費助成事業 基盤研究(C) 基盤研究(C)
横井 彩, 丸山 貴之, 江國 大輔, 森田 学, 山中 玲子
Grant amount：\3640000 （ Direct expense: \2800000 、 Indirect expense：\840000 ）
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gum chewing during postoperative fasting improve the prognosis after esophageal cancer surgery?
Grant number：19K10444 2019.04 - 2023.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
山中 玲子, 横井 彩, 森田 学, 丸山 貴之
Grant amount：\4290000 （ Direct expense: \3300000 、 Indirect expense：\990000 ）
本研究は，「術後絶食中のガム咀嚼が，食道がん手術後の予後を改善できるのか」という「問い」を検証するものである。
今年度は、研究計画の再検討、倫理委員会申請書類作成を行った。
科研申請時は、食道がん手術を受ける患者を対象に，術後絶食期間中に，従来の口腔衛生管理のみを行う「対照群」と，術後絶食中にガム咀嚼による口腔機能訓練を加えて行う「介入群」にランダムに割付け，2群間で比較して，食道がん術後絶食中のガム咀嚼による咀嚼機能や嚥下機能の維持効果，予後改善効果を検討する、としていた。
その後、具体的な研究方法について、倫理委員会、各科の医師・歯科医師、パラメディカル、統計専門家等と議論の結果、過去の我々の研究でえた結果を対照群とし、周術期にガム咀嚼を行う1群のみの介入研究とすることとなった。当初、2年半ほどの期間で目標症例数は70例程度と考えていたが、統計専門家との議論の結果、多くても40症例程度で結果がでることが明らかになった。そのため、当初の予定より、研究実施期間は短くて済む予定である。
現在、研究分担者・協力者、本学倫理委員会と協議し、書類作成を進めている。倫理委員会で承認され次第、研究を始める予定である。
なお、今のところ、新型コロナウイルス感染拡大に伴い、新たな研究開始の自粛が求められている。新型コロナウイルス感染予防対策には十分に配慮し、研究を進めていく方針である。 -
口腔機能訓練を含む歯科介入は術後頭頚部癌患者のQOLを改善できるか
Grant number：18K09909 2018.04 - 2021.03
日本学術振興会 科学研究費助成事業 基盤研究(C) 基盤研究(C)
横井 彩, 山中 玲子, 森田 学, 江國 大輔
Grant amount：\4290000 （ Direct expense: \3300000 、 Indirect expense：\990000 ）
本研究の目的は、頭頸部がんの治療を受けた患者における、口腔機能の改善と、QOLの改善との関係を明らかにすることである。2019年度は、頭頸部がん治療が、口腔機能の低下を介して、どのように治療後のQOLに影響を与えているかを検討した。
具体的には、頭頸部がん治療後の患者に対し、QOLを評価するアンケート（European organization for research and treatment of cancer quality of life core questionnaire-30）を実施した。また、口腔機能では、口の中の乾燥度、舌の力、口の開き具合、咬み合わせ、オーラルディアドコキネシス（1秒間に「パ」「タ」「カ」を発声できる回数）を評価した。さらに、がんのステージや、治療方法を評価した。得られた結果をもとに、QOLとその他の因子の関係を調べるため、パス解析を行った。
91名の頭頸部がん治療後の患者を評価した。そのうち75名の患者について分析を行った。平均年齢は64.5歳、男性43名（57.3％）であった。パス解析によって、頭頸部がんのステージが進行していること、および頸部郭清術・再建手術・化学療法を含むがん治療が、舌の力を低下させ、口を開けづらくさせ、「パ」「タ」「カ」を言いづらくさせることを介して、治療後のQOLを低下させることが明らかとなった。以上の結果により、術後のQOL改善に、舌の力や、口の開き具合、オーラルディアドコキネシスを改善させることが有効であることが示唆された。 -
Examination of a screening method using self-check sheet for perioperative oral management
Grant number：16K11857 2016.04 - 2020.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
Yoshitomi Aiko
Grant amount：\4550000 （ Direct expense: \3500000 、 Indirect expense：\1050000 ）
To promote multi-disciplinary team approach for perioperative oral management, we considered an efficient method using the simple self-check sheet, consisted a sentence “Do you have concerns with your mouth when undergoing surgery?”, which developed to assess requirements of professional dental interventions. To evaluate of the availability of the developed simple self-check sheet, the sensitivity and specificity were investigated. The results showed the simple self-check sheet had low sensitivity but high specificity. Therefore, patients, with some oral concerns by simple self-check sheet, should be examined and managed by dental professionals, at least. And routine pre-examination under general anesthesia by dental professionals is desirable for surgical safety, because of low sensitivity.
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Grant number：16K11858 2016.04 - 2020.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
Reiko Yamanaka
Grant amount：\4810000 （ Direct expense: \3700000 、 Indirect expense：\1110000 ）
In the esophageal cancer patients who underwent esophagectomy, we investigeted that the relationship between oral health status and prognosis index, the status of oral flora during neoadjuvant chemotherapy, the relationship between oral health status and nutritional status after surgery.
In consequence, these were suggested that the significant relationship between prognosis index and occlusal support, a tendency for decreased biodiversity in the patients who had oral mucosal disorder during neoadjuvant chemotherapy, and possibility of the positive relationship between exacerbations of nutritional status after surgery and occlusal support. -
Relationship between perioperative oral care and acetaldehyde consentration in mouth air
Grant number：15K11416 2015.04 - 2018.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
Yokoi Aya
Grant amount：\4550000 （ Direct expense: \3500000 、 Indirect expense：\1050000 ）
The purpose of the study is to investigate 1) the relationship between acetaldehyde concentration and microbiome on tongue, and 2) the related factors of tongue pressure decreasing and the effect of tongue excise. The relative abundance of Neisseria flacescens in high acetaldehyde group were significantly greater than those in the low group. The decline of tongue pressure significantly associated with length of intensive care unit (ICU) stay in patients after esophagectomy. The tongue pressure of patients who performed tongue excise were not significantly difference compared with no excise patients. In conclusion, the concentration of acetaldehyde in mouth air was related with Neisseria flavescens. The change of tongue pressure was associated with the length of ICU stay and was not effective from tongue excise in the patients after esophagectomy.
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The efficient oral management during perioperative period
Grant number：25862082 2013.04 - 2016.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B) Grant-in-Aid for Young Scientists (B)
Yamanaka Reiko
Grant amount：\2730000 （ Direct expense: \2100000 、 Indirect expense：\630000 ）
The purpose of this study was to clarify the relation between attitude about visiting dental clinic and demand of dental treatment before surgery, and to clarify oral characteristics of the patients in the department of Digestive tract surgery, Cranial nerve surgery, Respiratory surgery, Gynecology, and Brest thyroid surgery. Other purpose of this study was, in the patients who undergo esophageal surgery, to investigate relation between oral status and a prognostic factor, and the change of a prognostic factor during neoadjuvant chemotherapy.
As the results, the ratio of the patients who have to be treated before surgery was low in the patients who visit dental clonic at regular intervals, and the oral status of the patients who undergo Esophageal surgery was worse than that of the patients who undergo other surgery. The oral status in the patients who undergo Esophageal surgery was related with prognostic factor, and the change of a prognostic factor during neoadjuvant chemotherapy. -
Prevention of cancer by oral care inhibiting the production of acetaldehyde
Grant number：24593154 2012.04 - 2015.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
YOKOI AYA, YAMANAKA Reiko, MORITA Manabu, YAMASAKI Yutaka, KASHIWAZAKI Haruhiko, HATA Hironobu, TOMOFUJI Takaaki, TAMAKI Naofumi, EKUNI Daisuke, MARUYAMA Takayuki, SOGA Yoshihiko
Grant amount：\5070000 （ Direct expense: \3900000 、 Indirect expense：\1170000 ）
The aim of this study was to investigate the relationship between acetaldehyde concentration in mouth air and tongue coating volume in healthy volunteers. Acetaldehyde concentration in participants with a tongue coating score of 3 was significantly higher than those with a score of 1. After removing tongue coating, acetaldehyde concentration was significantly decreased.
Oral microflora on the tongue may be the main source of local production of acetaldehyde. Removing tongue coating may be a method inhibiting the production of acetaldehyde. -
Effectiveness of comprehensive food education (Shokuiku) against the lifestyle-related disease
Grant number：23593089 2011 - 2013
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
MARUYAMA Takayuki, MORITA Manabu, TOMOFUJI Takaaki, EKUNI Daisuke, YAMANAKA Reiko, TAKEUCHI Noriko
Grant amount：\4940000 （ Direct expense: \3800000 、 Indirect expense：\1140000 ）
Recently, it is noticed the importance of comprehensive food education (Shokuiku) against the lifestyle-related disease. In this study, we investigated whether eating until full, eating quickly, or both eating behaviors were associated with being overweight, the relationship between subjective and objective methods to evaluate eating quickly and to numerically characterize the kinesis of eating quickly. Also, we investigated the effects of intervention using a pamphlet or the combination of a pamphlet and dietary records on improvement of eating quickly. Furthermore, we investigated the relationship between knowledge about comprehensive food education and dental caries experience.
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Grant number：23792514 2011 - 2012
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B) Grant-in-Aid for Young Scientists (B)
YAMANAKA Reiko
Grant amount：\2860000 （ Direct expense: \2200000 、 Indirect expense：\660000 ）
Oral mucositis is one of side effect of radiation therapy and chemotherapy. The aim of this study was to clarify the preventive mechanism of oral mucositis from the view point of oxidative stress and microorganisms. As the results, oral mucositis had no relationship with oral microorganisms, oxidative stress in plasma and general status.
Moreover, oral mucositis had relationshipwith frequency of radiochemotherapy and chemotherapy more than oxidative stress in plasma. It was showed that initiation and severity of mucositis was not influenced by oxidative stress in plasma. -
Relationship between lipopolysaccharide and reactive oxygen species in rat periodontitis model
Grant number：18592277 2006 - 2007
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
YAMAMOTO Tetsuo, TOMOFUJI Takaaki, EKUNI Daisuke, YAMANAKA Reiko, WATANABE Tetsuo
Grant amount：\3880000 （ Direct expense: \3400000 、 Indirect expense：\480000 ）
Our previous study demonstrated that topical application of bacterial pathogens, i.e., lipopolysaccharide (LPS) and bacterial proteases, to gingival sulcus induced fatty liver. The purpose of this research project was to clarify the mechanism of fatty liver in rat periodontitis model, especially, to investigate the relationship between LPS and reactive oxygen species (ROS).
In the year 2006, serum levels of LPS, ROS and tumor necrosis factor (TNF)-alpha were found to be elevated in rats with periodontitis compared to rats without periodontitis. Moreover, both concentrations of 8-hydroxydeoxyguanosine (8-OHdG) in liver homogenate and densities of 8-OHdG-positive liver cells were larger in the rats with periodontitis than in those without These results suggest that topically applied ITS affects liver directly, that cells in the periodontium respond LPS and produce ROS, that the ROS produced in periodontium affects liver directly, and that cytokines play some roles in the pathogenesis of fatty liver induced by periodontitis.
In the year 2007, the effects of high cholesterol diet on the markers of oxidative stress in blood and liver of rats with periodontitis were investigated. Serum levels of reactive oxygen metabolites and hexanoyl-lysine and liver level of 8-OHdG were increased after feeding high cholesterol diet in the rats with periodontitis. These results suggest that high cholesterol diet has additive effects on periodontitis-induced oxidative damage in blood and liver.