Faculty Profiles - IMAMURA Koreyoshi

MISC - IMAMURA Koreyoshi

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Effects of the Cultivation Method on the Characteristics or Gene Expression Profiles of Aspergillus oryzae Using mCD or DPY Media

Imanaka Hiroyuki, Tanaka Soukichi, Feng Bin, Imamura Koreyoshi, Nakanishi Kazuhiro

Memoirs of the Faculty of Engineering, Okayama University 44 73 - 83 2010.1

2P-1103 Influence of amino acid sequence on its translocation to E. coli cell surface by autotransporter

IMANAKA Hiroyuki, UEDA Hisako, IMAMURA Koreyoshi, NAKANISHI Kazuhiro

22 34 - 34 2010

3P-1055 Multiformity of β-Galactosidase Produced from Bacillus circulans :

SONG Jingyuan, IMANAKA Hiroyuki, IMAMURA Koreyoshi, MINODA Masashi, YAMAGUCHI Shoutaro, NAKANISHI Kazuhiro

22 71 - 71 2010

3P-1085 Identification of a key amino acid residue for the formation of cysteine synthase complex using a highly functional peptide immobilization

SHIMIZU Yuki, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro

22 79 - 79 2010

MN-P23 Development of peptide immobilization method using PS-tagged cushion protein(Section X Micro/Nano Technology for Analysis and Cell Manipulation)

Imanaka Hiroyuki, Yamazumi Daisuke, Kunikata Toshinobu, Imamura Koreyoshi, Nakanishi Kazuhiro

Journal of bioscience and bioengineering 108 ( 1 ) S161 2009.11

Efficient Ethanol Production from Wheat Bran by Enzymatic Saccharification Using Commercially Available Enzyme Products and Fermentation Using Bakers' Yeast

KOREISHI Mayuko, IMANAKA Hiroyuki, IMAMURA Koreyoshi, KARIYAMA Masahiro, NAKANISHI Kazuhiro

Seibutsu-kougaku Kaishi 87 ( 5 ) 216 - 223 2009.5

Language：Japanese Publisher：日本生物工学会

We aimed to produce ethanol efficiently by enzymatic saccharification coupled with ethanol fermentation using wheat bran as a biomass. For this purpose, we examined pretreatment conditions of biomass and a combination of commercially available enzyme products for saccharification. When a 3.3% (w/v) wheat bran suspension was pretreated using subcritical water at 140℃ with a holding time of 0h, followed by saccharification at 45℃ using a mixture of commercially available Meiselase (composed of cellulase, cellobiase/β-glucosidase, and xylanase) and Novozyme^[○!R] 188 (composed of cellobiase/β-glucosidase, α-amylase, and glucoamylase) at a ratio of 4 to 1 by weight, glucose was released at 10.6g/l, which corresponded to 90% of the value calculated on the basis of the total amount of glucose-based saccharides contained in the wheat bran. On the other hand, from a pretreated 33% (w/v) wheat bran suspension, 85g/l glucose was produced, which corresponded to only 72% of the theoretical value, owing to the inhibitory effect of glucose on β-glucosidase activity. However, when fermentation was carried out at 30℃ following 24-h saccharification of the pretreated wheat bran using a mixture of Meiselase and Novozyme^[○!R] 188, the inhibitory effect of glucose was considered to be reduced and as a result, after 21-h fermentation, ethanol was produced at 5.2% (w/v), which corresponded to 88% of the theoretical maximum value.

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2Ea05 Investigation of peptide surface display system by autotransporter

UEDA Hisako, YAMASHITA Mai, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro

21 59 - 59 2009

2Ip05 Investigation of Peptide-Protein Interaction Detection System by Cushion Protein

IMANAKA Hiroyuki, KUNIKATA Toshinobu, YAMANOUCHI Mai, IMAMURA Koreyoshi, NAKANISHI Kazuhiro

21 126 - 126 2009

2Da01 Construction of expression systems of a novel aminoacylase from Streptomyces mobaraensis

OOI Manami, NAKATANI Yasuyuki, KOREISHI Mayuko, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro

21 44 - 44 2009

3Ga07 Development of rapid and sensitive detection system of human FOXP3-DNA interaction

MAEKAWA Masami, IMANAKA Hiroyuki, IMAMURA Koreyoshi, KONDO Eisaku, NAKANISHI Kazuhiro

20 186 - 186 2008

2Aa03 Purification and Characterization of Recombinant Monoacylglycerol Lipase from Rhodanobacter sp. strain LP7315

TERAKAWA Shuji, MORI Masashi, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro

20 59 - 59 2008

3Da04 Immobilization of Biomolecules by Cushion Protein on Solid Surfaces

IMANAKA Hiroyuki, YAMAZUMI Daisuke, YANAGITA Keisuke, IMAMURA Koreyoshi, NAKANISHI Kazuhiro

20 126 - 126 2008

Enzymatic modification of proteolytic fragments of food proteins with fattyacid using a novel acylase

Annual report of study for food under the supporting program by The Skylark Food Science Institute ( 21 ) 109 - 115 2008

Enzymatic synthesis of beta-lactam antibiotics and N-fatty-acylated amino compounds by the acyl-transfer reaction catalyzed by penicillin V acylase from Streptomyces mobaraensis

Mayuko Koreishi, Kazuha Tani, Yuuichi Ise, Hiroyuki Imanaka, Koreyoshi Imamura, Kazuhiro Nakanishi

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 71 ( 6 ) 1582 - 1586 2007.6

Language：English Publisher：TAYLOR & FRANCIS LTD

Penicillin V acylase from Streptomyces mobaraensis (Sm-PVA) showed high acyl-transfer activity in reactions using methyl esters of carboxylic acid (acyl donor) and amino compounds (nucleophile), to produce the corresponding amides. Moreover, Sm-PVA had broad substrate specificity, as indicated by the fact that it catalyzed the efficient synthesis of fl-lactam antibiotics, capsaicin derivatives, and N-fatty-acyl-amino acid/N-fatty-acyl-peptide derivatives.

DOI： 10.1271/bbb.70052

Web of Science

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1C11-2 Enzymatic synthesis of functional compounds by the acyl-transfer reaction catalyzed by penicillin V acylase from Streptomyces mobaraensis

KOREISHI Mayuko, ISE Yuuichi, TANI Kazuha, IMANAKA Hiroyuki, IMAMURA Koreyoshi, NAKANISHI Kazuhiro

19 79 - 79 2007