Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1021/acs.orglett.2c00370

J-GLOBAL

researchmap

Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

We have developed an efficient reaction of 3-azido-2-methoxyindolines with <italic>O</italic>-nucleophiles in the presence of a Lewis acid, affording 2-monoacyloxy or alkoxy indolin-3-ones.

DOI： 10.1039/d2qo00048b

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ROYAL SOC CHEMISTRY  

We introduce readily available ammonium hemiaminals as O-transfer reagents and commercially available acetonitriles as a primary amide enolate precursor. The combination serves as an amide enolate equivalent, thereby providing one-pot access to α-substituted indolylacetamides. A broad substrate scope and good functional group tolerance as well as gram-scale synthesis make this protocol highly attractive. Mechanistic experiments suggest that the cyano group is trapped by a hydroxy group of hemiaminals en route to the desired primary amides under metal-free conditions.

DOI： 10.1039/d1cc02821a

Web of Science

Scopus

PubMed

researchmap

Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

Use of 3-azidoindoles in organic synthesis remains difficult task owing to their instabilities. Herein, we report a general and concise approach for tackling this problem by using 3-azidoindole surrogates. The...

DOI： 10.1039/d1cc06033c

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-V C H VERLAG GMBH  

Superior pH-responsive molecules are required for the development of functional materials applicable to advanced molecular technologies. Despite having been widely developed, many rhodamine-based pH-responsive molecules exhibit a single configurational switch for "turn-on." Herein, we report a new type of rhodamine-based pH-responsive molecule with multi-configurational switches displaying stable two-step structural and color conversion in response to pH. This rhodamine analog could be successfully applied to optical sensing of pH gradient under extreme acidic environments both in solution and on hydrogel through high-contrast color change. We demonstrated that this multi-responsive character enabled optical memory of different pH information.

DOI： 10.1002/chem.202004254

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ROYAL SOC CHEMISTRY  

The research and development of multi-stimuli, multi-responsive molecules have attracted considerable attention in chemistry, biology, and material science. Herein, we propose a multi-stimuli-responsive multi-fluorescence system in a single molecule. This system is based on the isomerization that involves ring-opening/closing reactions of spirolactones of rhodamine analogues (ABPXs), developed by our group, which can be independently controlled by light and chemical stimuli. UV light irradiation opens one of the spirolactones to give thermally stable coloured isomer (Z) in solution. Detailed synthetic and theoretical investigations reveal that the ring-opening reaction of ABPXs proceeds via the formation of a photo-induced charge separated state, followed by the recombination of the biradicals. Furthermore, we explore the structure-kinetic relationships and demonstrate that the introduction of electron-donating substituents into the xanthene ring can tune the lifetime of the photo-generated isomer. Chemical stimulation by an acid further promotes the ring-opening reaction to give a red-shifted isomer (D). These light and chemical input signals can be converted into output signals of distinct colour and fluorescence. The multi-stimuli-responsive multi-colour fluorescence could be distinctively expressed through the combinatorial logic gate.

DOI： 10.1039/c9tc05054j

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

A new procedure for the iron-catalyzed synthesis of enamides from ketoximes was developed, and its mechanism was proposed. A unique reduction system, with the concerted use of KI and Na2S2O4, was involved. The reaction exhibited a wide substrate scope and gave good yields in a short reaction time. The procedure is operationally simple and also applicable for the large-scale synthesis. (C) 2019 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tetlet.2019.05.006

Web of Science

Scopus

researchmap

Publishing type：Research paper (scientific journal)  

Novel aminobenzopyranoxanthene spiroethers (ABPX-SEs) based on the spirocyclization of the hydroxymethyl group were synthesized from ABPX spirolactones (ABPX-SLs). The addition of an acid induces a ring-opening reaction to yield two colored monocationic and dicationic spiro-ring species of ABPX. The acid-stimulus responsiveness of the ABPX-SEs is lower than that of ABPX-SLs in polar organic solvents. In addition, the ABPX-SEs exhibit stepwise structural changes of the three equilibrium species at acidic pH, although rapid conversion from the spirolactone to dicationic species occurs in the case of ABPX-SLs.

DOI： 10.3987/COM-18-S(F)66

Scopus

researchmap

Publishing type：Research paper (scientific journal)  

We developed a new reaction for the one-pot synthesis of a fused cyclic compound from triphenylguanidine, which was thought to be tandem cyclization, with a moderate yield. In this reaction, conditions such as the metal reagent, oxidant, solvent, and ligand affected whether single or tandem cyclization was preferred.

DOI： 10.3987/COM-18-S(F)61

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

We report synthesis and characterization of near-infrared (NIR)-absorbing/emitting Thiele's hydrocarbon derivatives, in which four aryl groups are bridged to a quinodimethane skeleton. The quinoid structure of the bridged-tetra-aryl- p-quinodimethanes (BTAQs) was confirmed by spectroscopic, X-ray crystallographic, and computational methods. Although quinodimethane derivatives with a small HOMO-LUMO energy gap often exhibit biradical character, BTAQs showed no biradical character. Instead, they exhibited two-step near-infrared electrochromism. The donor/acceptor properties of the aryl groups were found to play a key role in the unique properties of BTAQs.

DOI： 10.1021/jacs.8b11092

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ROYAL SOC CHEMISTRY  

Both 2- and 2-hydroxypropyl substituted 14-epi-1,25-dihydroxy-19-nortachysterols were synthesized to study the human vitamin D receptor (hVDR) binding affinity, binding configurations, and interactions with amino acid residues in the ligand binding domain of hVDR by X-ray co-crystallographic analysis. In conjunction with our previous results on 14-epi-19-nortachysterol, 2-methylidene-, 2-methyl-, 2-methyl, and 2-hydroxypropoxy-14-epi-19-nortachysterol, we propose a variety of effects of substitution at the C2 position in the 14-epi-19-nortachysterol skeleton on biological activities.

DOI： 10.1039/c8ob00158h

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

An efficient and practical method for the synthesis of a variety of aminobenzopyranoxanthenes (ABPXs) with different nitrogen containing fused rings was developed. On the basis of the mechanistic studies of the formation of the xanthene framework, the presented methodology was developed to facilitate access to previously inaccessible asymmetric ABPXs.

DOI： 10.1021/acs.joc.7b02290

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

The direct C-H substitution reactions of anilides using hypervalent iodine proceeded to afford azide, chloro, bromo, and fluoro derivatives, and their regioselectivity were described. In the specific reaction conditions, the unique regioisomers were obtained. (C) 2017 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tetlet.2017.08.079

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

According to the binding mode of 14-epi-1 alpha,25-dihydroxy-19-nortachysterol in the ligand binding domain of human vitamin D receptor (hVDR), i.e., 5,6- and 7,8-s-trans configuration that was shown by X-ray co-crystallographic analysis, 7,8-cis-locked 1 alpha,25(OH)(2)D-3 analogs were synthesized. In this paper, the synthesis and biological activity of 2 alpha- and 2 beta-(3-hydroxypropyl)-7,8-cis-14-epi-1 alpha,25-dihydroxy-19-norvitamin D-3 are reported. The A-ring and CD-ring precursors for the Julia-Kociensky coupling reaction to create a diene system of the target molecules were prepared using our original methods. hVDR binding affinity and osteocalcin promoter transactivation activity of the new 7,8-cis-14-epi-vitamin D-3 analogs were evaluated. Interestingly, the 2 beta-substituted 7,8-cis-analog was a better binder for hVDR than the 2 alpha-isomeric counterpart. (C) 2016 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jsbmb.2016.09.007

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

Hereditary vitamin D-resistant rickets (HVDRR) is caused by mutations in the vitamin D receptor (VDR) gene. Arg274 located in the ligand binding domain (LBD) of VDR is responsible for anchoring 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)(2)D-3) by forming a hydrogen bond with the 1 alpha-hydroxyl group of 1 alpha,25(OH)(2)D-3. The Arg274Leu (R274L) mutation identified in patients with HVDRR causes a 1000-fold decrease in the affinity for 1 alpha,25(OH)(2)D-3, and dramatically reduces vitamin D-related gene expression. Recently, we successfully constructed fusion proteins consisting of split-luciferase and LBD of the VDR. The chimeric protein LucC-LBD-LucN, which displays the C-terminal domain of luciferase (LucC) at its N-terminus, can detect and discriminate between VDR agonists and antagonists. The LucC-LBD (R274L)LucN was constructed to screen high-affinity ligands for the mutant VDR (R274L). Of the 33 vitamin D analogs, 5 showed much higher affinities for the mutant VDR (R274L) than la,25(OH)2D3, and 2 alpha-[2-(tetrazol-2-ypethy1]-1 alpha,25-(OH)(2)D-3 showed the highest affinity. These compounds might be potential therapeutics for HVDRR caused by the mutant VDR (R274L). (C) 2016 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jsbmb.2016.11.008

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ROYAL SOC CHEMISTRY  

While investigating the unique optical properties of aminobenzopyranoxanthenes (ABPXs), organic fluorescent dyes with the fusion of two rhodamines, we have found that the spirolactone form of ABPXs exhibited solvatochromic fluorescence in organic solvents. Detailed spectrophotometric and theoretical analyses showed that the solvatochromic fluorescence of ABPXs originated from the photo-excited charge separation in solvents of different dipolarities. Further studies revealed that fluorescent nano-aggregates were also formed in highly concentrated solution. The intriguing dual fluorescence properties of ABPXs were tunable in response to the water content, and served as a new detection principle for naked-eye visualisation (above 0.5 wt%) and quantification (0.010-0.125 wt%) of water in tetrahydrofuran.

DOI： 10.1039/c6cp06808a

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

Aminobenzopyranoxanthene (ABPX) dyes containing linear n-alkyl chains at amino groups were synthesized, and their cis and trans stereoisomers were isolated. Detailed spectrophotometric studies revealed that all the ABPX derivatives exhibited fluorescence emission in the far-red and near-infrared wavelength regions, and their fluorescence quantum efficiency increased with increasing n-alkyl chain length. Almost no differences in photophysical properties were observed between the cis and trans stereoisomers.

DOI： 10.3987/COM-16-S(S)65

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

Fluorans are popular leuco dyes that are used in various applications, such as carbonless-copy papers and thermal papers. Here, we describe unique leuco dyes in which two fluoran units are fused into a C-2h structure (iso-aminobenzopyranoxanthenes; iso-ABPXs). iso-ABPXs exhibited a large two-step color change (colorless/pink and pink/blue-green) due to opening closing of two spirolactone rings. The two-step equilibrium among the colorless, pink, and blue-green forms could be well controlled by adjusting acid concentration, solvent, and/or temperature.

DOI： 10.1021/acs.joc.6b02403

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

Four 7,8-cis-1 alpha,25-dihydroxyvitamin D-3 derivatives, 7,8-cis- and 7,8-cts-14-epi-1 alpha,25-dihydroxy-19-norvitamin D-3 as well as 7,8-cis- and 7,8-cis-14-epi-1 alpha,25-dihydroxyvitamin D3 were synthesized, and their chemical stability was characterized. In our previous work, we disclosed that 14-epi-19-nortachysterol showed the unprecedented binding configuration in human vitamin D receptor (hVDR), that is, 5,6- and 7,8-s-trans configuration. However, this configuration is variable because of the rotation at the single bond between C7 and C8. For the precise discussion of the 7,8-s-trans configuration, we designed and synthesized the 7,8-cis-locked skeleton of vitamin D-3 derivatives. Among four analogs, the 19-nor derivatives were stable at ambient temperature, and their hVDR binding affinity and co-crystallographic analysis of their hVDR complexes were studied. The other derivatives with the triene system were isomerized to corresponding previtamin D-3 and vitamin D-3. (C) 2016 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tet.2016.03.081

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

Mechanochromic organic molecules (MOMs) that exhibit a large difference of fluorescence wavelength between two states have important potential applications, but few such compounds are known. Here, we report a new MOM, cis-ABPX01(0), which shows switchable near-IR and blue fluorescence responses. Detailed spectrophotometric and single-crystal X-ray analyses revealed that the near-IR fluorescence is attributable to fluorescence from slip-stacked dimeric structures in crystals, while the blue fluorescence is attributable to fluorescence from the monomer. Switching between the two is achieved by dynamic structural interconversion between the two molecular packing arrangements in response to mechanical grinding and solvent vapor-fuming.

DOI： 10.1021/jacs.5b00877

Web of Science

Scopus

PubMed

researchmap

Language：English   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

Three different A-ring perhydroxylated trihydroxyvitamin D-3 metabolites were synthesized from their appropriate A-ring precursors and CD-ring for their potential therapeutic applications. We first chemically synthesized 1 alpha,2 alpha,25-trihydroxyvitamin D-3 [1 alpha,2 alpha,25(OH)(3)D-3] to study its VDR binding affinity because this metabolite is a product of recombinant human CYP3A4 catalysis when 2 alpha-(3-hydroxypropoxy)-1 alpha,25-dihydroxyvitamin D-3 (O2C3), a more potent vitamin D receptor (VDR) binder than 1 alpha,25-dihydroxyvitamin D-3 [1 alpha,25(OH)(2)D-3], is used as the substrate. We found that this metabolite retained 27.3% of the VDR binding affinity compared to 1 alpha,25(OH)(2)D-3. The k(cat)/K-m value of CYP24A1 for 1 alpha,2 alpha,25(OH)(3)D-3 is 60% of that for 1 alpha,25(OH)(2)D-3. Since the biological activity and the metabolic fate of a naturally occurring C4-hydroxylated vitamin D-2 metabolite found in the serum of rats treated with pharmacological doses of vitamin D-2 have never been described, we next synthesized 1 alpha,4 alpha,25-trihydroxyvitamin D-3 and its diastereoisomer, 1 alpha,4 beta,25-trihydroxyvitamin D-3, to study their metabolism and biological activities. Both 4-hydroxylated isomers showed weaker VDR binding affinity than 1 alpha,25(OH)(2)D-3. Although either 4-hydroxylated isomer can be metabolized by CYP24A1 almost at the same level as 1 alpha,25(OH)(2)D-3, their metabolic patterns catalyzed by uridine 5'-diphosphoglucuronosyltransferase (UGT) are different; only the 4 alpha-hydroxylated analog can be metabolized by UGT to produce a glucuronate conjugate. The results provide important information for the synthesis of new novel chemotherapeutic vitamin D analogs which would be less subjective to degradation and therefore more bioavailable than 1 alpha,25(OH)(2)D-3.This article is part of a Special Issue entitled '17th Vitamin D Workshop'. (C) 2014 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jsbmb.2014.09.021

Web of Science

Scopus

PubMed

researchmap

Language：Japanese   Publishing type：Research paper (scientific journal)  

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

Four new 19-norvitamin D-3 analogs, 2 alpha-[3-(tetrazol-1-yl)propyl]-, 2 beta-[3-(tetrazol-1-yl)propyl]-, 2 alpha-[3-(tetrazol-2-yl)propyl]-, and 2 beta-[3-(tetrazol-2-yl)propyl]-1 alpha,25-dihydroxy-19-norvitamin D-3 were synthesized. Among them, 2 alpha-[3-(tetrazol-1-yl)propyl] -1 alpha,25-dihydroxy-19-norvitamin D-3 showed weak binding affinity for vitamin D receptor (VDR) (2.6% of 1 alpha,25-dihydroxyvitamin D-3: ca. 15% of 1 alpha,25-dihydroxy-19-norvitamin D-3) and weak VDR transactivation activity in human osteosarcoma cells, which was determined by luciferase assays (EC50 7.3 nM, when 1 alpha,25-dihydroxyvitamin D-3 0.23 nM). Although the other three compounds could not act as VDR binders by evaluation of the competition assays, 2 alpha[3-(tetrazol-2-yl)propyl] analog showed weak transactivation activity (EC50 12.5 nM).

DOI： 10.3987/COM-14-S(K)108

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：BENTHAM SCIENCE PUBL LTD  

As the first stable tachysterol analogs, 14-epi-19-nortachysterol and its 2-substituted derivatives were synthesized using the Stille coupling reaction between the A-ring precursor (three vinylstannanes) and the CD-ring vinyl trifrate. Among them, the 2-methylidene group was hydrogenated with Wilkinson's catalyst regioselectively to obtain 2 alpha -and 2 beta-methyl analogs after separation; therefore, five new 14-epi-19-nortachysterols were constructed. All 14-epi-19-nortachysterols showed moderate to strong human vitamin D receptor (hVDR) binding affinity except the 2 alpha-(3-hydroxypropoxy) substituted analog. X-ray cocrystallographic analysis of the [truncated hVDR]-[2-methyl-14-epi-19-nortachysterol] complex exhibited an unusual binding structure that has not been observed previously.

DOI： 10.2174/1568026615666141208103741

Web of Science

Scopus

PubMed

researchmap

Language：English   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

X-ray cocrystallographic studies of the human vitamin D receptor (hVDR)-[2 alpha-(3-hydroxypropyl)-1 alpha,25-dihydroxyvitamin D-3 (01C3)] complex showed that the terminal hydroxy group of the 2 alpha-functional group of 01C3 formed a hydrogen bond with Arg274 in the ligand binding domain (LBD) of hVDR to stabilize the complex; therefore, 01C3 showed 3-times greater binding affinity for VDR than the natural hormone. Here, the effects of a heteroaromatic ring on binding to hVDR instead of the terminal OH group of 01C3 and also on preliminary biological activities were studied. We synthesized 2 alpha-[2-(tetrazol-2-yl)ethyl]-1 alpha,25(OH)(2)D-3 (1a) and its regioisomer 2 alpha-[2-(tetrazol-1-yl)ethyl]-1 alpha,25(OH)(2)D-3 (1b), in which 1a showed much higher hVDR binding affinity and greater osteocalcin promoter transactivation activity in human osteosarcoma (HOS) cells than those of 1b. X-ray cocrystallographic analysis of the hVDR-1a complex showed new hydrogen bond formation between one of the nitrogen atoms of the tetrazole ring and Arg274. (C) 2013 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jsbmb.2013.09.001

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：2  

Our previous studies revealed that recombinant human CYP3A4 converted 2 alpha-(3-hydroxypropoxy)-1 alpha,25-dihydroxyvitamin D-3 (O2C3), which was a more potent binder to vitamin D receptor (VDR) than the natural hormone, 1 alpha,25-dihydroxyvitamin D-3 (1 alpha,25(OH)(2)D3, 1), to 1 alpha,2 alpha,25-trihydroxyvitamin D-3 (2). Here, we synthesized 2 using the Trost Pd-mediated coupling reaction between an A-ring precursor and a CD-ring bromoolefin and evaluated its preliminary biological activity. We found that metabolite 2 from O2C3 was still active as a VDR ligand while maintaining human VDR binding affinity (27.3% of 1 alpha,25(OH)(2)D-3) and HL-60 cell differentiation activity (62% of 1 alpha,25(OH)(2)D-3).

DOI： 10.1248/cpb.c13-00646

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：7  

2 alpha-Heteroarylethyl-1 alpha,25-dihydroxyvitamin D-3 analogues, which were designed to form a hydrogen bond between Arg274 of human vitamin D receptor (hVDR) and a nitrogen atom of the heteroaromatic ring at the 2 alpha-position, were synthesized. Among them, 2 alpha-[2-(tetrazol-2-yl)ethyl]-1 alpha,25-dihydroxyvitamin D-3 showed higher osteocalcin promoter transactivation activity in human osteosarcoma (HOS) cells and a greater therapeutic effect in ovariectomized (OVX) rats, osteoporosis model animals, on enhancing bone Mineral density than those of active vitamin D-3. X-ray cocrystallographic analysis of the hVDR-ligand complex confirms that the new hydrogen bond formation stabilized the complex.

DOI： 10.1021/ml400098w

Web of Science

Scopus

PubMed

researchmap

Language：English   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

Recently, we evaluated a novel skeleton in the vitamin D family, 14-epi-1 alpha,25(OH)(2)-19-nortachysterol, and discovered its unique binding configuration in the human vitamin D receptor (VDR) with the C5,6- and C7,8-s-trans triene configuration. Because of its unprecedented form, this skeleton has a promising characteristic profile for clinical use, and also the synthesis of its derivatives should be versatile. Therefore, we synthesized the novel analog, 2 alpha-hydroxypropoxy substituted 14-epi-1 alpha,25(OH)(2)-19-nortachysterol, and evaluated its human VDR binding affinity. Although this substitution is one of the promising modification of vitamin D-3 such as eldecalcitol (ED-71), it had negative effects on the binding affinity, and the compound showed lower affinity than 1 alpha,25(OH)(2)D-3 and its parent compound, 14-epi-1 alpha,25(OH)(2)-19-nortachysterol. It was thought that the unprecedented binding configuration of this skeleton should not allow the terminal hydroxyl group of the 2 alpha-substituent to construct effective hydrogen bond networks around the amino acid residues in the binding pocket.This article is part of a Special Issue entitled 'Vitamin D Workshop'. (c) 2012 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jsbmb.2012.11.014

Web of Science

Scopus

PubMed

researchmap

Language：English   Publisher：16  

The active form of vitamin D-3, 1 alpha,25(OH)(2)D-3 or calcitriol, is known to inhibit the proliferation and invasiveness of many types of cancer cells, including prostate and liver cancer cells. These findings support the use of 1 alpha,25(OH)(2)D-3 for prostate and liver cancer therapy. However, 1 alpha,25(OH)(2)D-3 can cause hypercalcemia, thus, analogs of 1 alpha,25(OH)(2)D-3 that are less calcemic but exhibit potent antiproliferative activity would be attractive as therapeutic agents. We have developed 2 alpha-functional group substituted 19-norvitamin D-3 analogs with and without 14-epimerization. Among them, 2 alpha- and 2 beta-(3-hydroxypropyl)-1 alpha,25-dihydroxy-19-norvitamin D-3 (MART-10 and -11, respectively) and 14-epi-2 alpha- and 14-epi-2 beta-(3-hydroxypropyl)-1 alpha,25-dihydroxy-19-norvitam in D-3 (14-epi-MART-10 and 14-epi-MART-11, respectively) were found to be the most promising. In this review, we discuss the synthesis of this unique class of vitamin D analogs, the molecular mechanism of anticancer actions of vitamin D, and the biological evaluation of these analogs for potential application to the prevention and treatment of prostate and liver cancer.

DOI： 10.4155/fmc.12.130

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：1  

The Delta(16) structure as a vitamin D analog enhanced vitamin D receptor (VDR) binding affinity and induced significant cell differentiation, whereas its relative calcemic activity was reduced compared to 1 alpha,25-dihydroxyvitamin D-3 (1 alpha,25(OH)(2)D-3). Methodologies available to introduce a double bond at C16-C17 of the D-ring on the seco-steroidal skeleton were limited; therefore, a new synthetic strategy was developed to obtain not only the Delta(16) structure, but also a new C15 functional group. Since C15-functionalization was unprecedented in vitamin D analog studies, the hybrid structure of Delta(16) and the C15-OH group at the D-ring may provide important information on the structure-activity relationship with vitamin D analogs. The synthesized 16-ene-2 alpha-methyl-1 alpha,15 alpha,25-trihydroxyvitamin D-3 showed almost 3-times higher VDR binding affinity and an equipotent level of osteocalcin promoter transactivation activity in human osteosarcoma cells as compared to 1 alpha,25(OH)(2)D-3.

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：1  

The synthesis of 14-epi-1 alpha,25(OH)(2)previtamin D-3, 14-epi-19-nor-1 alpha,25(OH)(2)previtamin D-3, and their 2-substituted analogs is described. The vitamin D receptor (VDR) binding affinity was further evaluated and 2 alpha-methyl substituted 14-epi-1 alpha,25(OH)(2)previtamin D-3 had 17-fold more potent affinity than 14-epi-1 alpha,25(OH)(2)previtamin D-3. In the comparison of these compounds, the effects of thermal equilibrium, with or without 19-carbon at the A-ring, and their CD-ring structures are discussed.

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：10  

A previous report has demonstrated the existence of a C4-hydroxylated vitamin D-2 metabolite in serum of rats treated with pharmacological doses of vitamin D-2. However, the biological significance and metabolic fate of this metabolite have not been described. To explore its potential biological activities, we therefore synthesized 1 alpha,4 alpha,25-trihydroxyvitamin D-3 and its diastereoisomer, 1 alpha,4 beta,25-trihydroxyvitamin D-3, using Trost Pd-mediated coupling reaction, and studied their vitamin D receptor (VDR) binding affinity, osteocalcin promoter transactivation activity, and their further metabolism by human CVP24A1 as well as by human liver microsomal fraction based on CVP- and UDP-glucuronosyltransferases (UGTs)-reactions.

DOI： 10.1248/cpb.c12-00526

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

Multivalency is a common principle in the recognition of cellular receptors, and multivalent agonists and antagonists have played a major role in understanding mammalian cell receptor biology. The study of bacterial cell receptors using similar approaches, however, has lagged behind. Herein we describe our efforts toward the development of a dendrimer-based multivalent probe for studying AI-2 quorum-sensing receptors. From these studies, we have discovered a chemical probe specific for Lsr-type AI-2 quorum-sensing receptors with the potential for enabling the identification of new bacterial species that utilize AI-2 as a quorum-sensing signaling molecule.

DOI： 10.1021/ja207556d

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：11  

C15-Substituted 1 alpha,25-dihydroxyvitamin D-3 analogs were synthesized for the first time to investigate the effects of the modified CD-ring on biological activity concerning the agonistic positioning of helix-3 and helix-12 of the vitamin D receptor (VDR). X-ray cocrystallographic analysis proved that 0.6 angstrom shifts of the CD-ring and shrinking of the side chain were necessary to maintain the position of the 25-hydroxy group for proper interaction with helix-12. The 15-hydroxy-16-ene derivative showed higher binding affinity for hVDR than the natural hormone.

DOI： 10.1021/ol200828s

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

In the study of the synthesis of 14-epi-19-norprevitamin D-3, we found 14-epi-19-nortachysterol derivatives through C6,7-cis/trans isomerization. We also succeeded in their chemical synthesis and revealed their marked stability and potent VDR binding affinity. To the best of our knowledge, this is the first isolation of stable tachysterol analogues. Surprisingly, 14-epi-19-nortachysterol derivatives exhibited an unprecedented binding configurations for the ligand binding pocket in hVDR, C5,6-s-trans and C7,8-s-trans triene configurations, which were opposite the natural C7,8-ene-configuration of 1 alpha,25(OH)(2)D-3.

DOI： 10.1021/ja201481j

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

DOI： 10.1021/jm901742e

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

2 alpha- and 2 beta-Substituted analogs of 14-epi-previtamin D-3 were synthesized and isolated after thermal isomerization of 14-epi-vitamin D-3 triene at 80 degrees C. The VDR binding affinity and transactivation activity of osteocalcin promoter in HOS cells were tested, and the 2 alpha-methyl-substituted analog was found to have greater genomic activity than 14-epi-previtamin D-3. We found that modification at the C2 position of the seco-steroidal skeleton afforded interesting effects for biological genomic activity for the previtamin D form as well as the natural vitamin D form. (C) 2010 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tet.2010.05.028

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

Two derivatives of 14-epi-1 alpha,25-Dihydroxy-19-norvitamin D-3, 14-epi-2 alpha-(3-hydroxypropyl)-1 alpha,25-dihydroxy-19-norvitamin D-3 (14-epi-MART-10) and its 2-epimeric analog (14-epi-MART-11), were synthesized using Julia coupling reaction to connect between the C5 position (steroidal numbering) of an A-ring precursor ketone derived from (-)-quinic acid and the C6 position of a protected 14-epi-CD-ring benzothiazole sulfone. The coupling and deprotection reactions generated a mixture of 14-epi-MART-10 and 14-epi-MART-11 in a moderate yield. The C2-isomers were then separated as their pivalate forms. The C2-stereochemistry of 2 alpha- and 2 beta-isomers was determined by H-1 NMR studies including NOE experiments. The pivaloyl group was removed under basic conditions to obtain the target molecules of 14-epi-MART-10 and 14-epi-MART-11. The metabolism of these two new analogs was further studied in a reconstituted cell-free human CYP24A1 system to elucidate the potential mechanism of their super agonistic action on vitamin D receptor. Our results indicate that epimerization at C14 makes the analogs less susceptible to CYP24A1 degradation and therefore more bio-available, leading to enhanced biological activities.

DOI： 10.3987/COM-10-S(E)40

Web of Science

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

2 beta-Substituted analogs of 14-epi-previtamin D-3 were synthesized for the first time by the thermal isomerization of the corresponding 14-epi-vitamin D-3 that were available using coupling reaction between the A-ring phosphine oxide derived from a chiral epoxide and CD-ring cis-hydrindanone. The VDR binding affinity and transactivation activity of osteocalcin promoter in HOS cells were evaluated, and the new analogs were found to be less active, 0.01-0.18% of VDR binding affinity compared with the natural hormone and EC50 1.0-9.1 nM for transactivation activity, than 14-epi-previtamin D3 with 0.5% (VDR) and EC50 0.46 nM, respectively. (C) 2010 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jsbmb.2010.02.035

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

Quorum sensing (QS) systems have been discovered in a wide variety of bacteria, and mediate both intra- and interspecies communication. The AI-2-based QS system represents the most studied of these proposed interspecies systems and has been shown to regulate diverse functions such as bioluminescence, expression of virulence factors, and biofilm formation. As such, the development of modulatory compounds, both agonists and antagonists, is of great interest for the study of unknown AI-2-based QS systems and the potential treatment of bacterial infections. The fimbrolide class of natural products has exhibited excellent inhibitory activity against AI-2-based QS and as such may be considered the "gold standard" of AI-2 inhibitors. Thus, we sought to include a fimbrolide as a control compound for our recently developed alkyl-DPD panel of AI-2 modulators. Herein, we present a revised synthesis of a commonly studied fimbrolide as well as a direct comparison between the fimbrolide and alkyl-DPD analogues. We demonstrate that our alkyl-DPD analogues are more potent inhibitors of QS in both Vibrio harveyi and Salmonella typhimurium, the two organisms with defined AI-2 QS systems, and in doing so call into question the widely accepted use of fimbrolide-derived compounds as the "gold standard" of AI-2 inhibition.

DOI： 10.1021/ja9066783

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

We synthesized and isolated 2 alpha-substituted analogs of 14-epi-previtamin D-3 after thermal isomerization at 80 degrees C for the first time. The VDR binding affinity and transactivation activity of osteocalcin promoter in HOS cells were evaluated, and the 2 alpha-methyl-substituted analog was found to have greater genomic activity than 14-epi-previtamin D-3. (C) 2009 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmcl.2009.07.112

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：INT INST ANTICANCER RESEARCH  

The 14-epimer of MART-10, namely 14-epi-MART-10 (14-epi-2 alpha-(3-hydroxypropyl)-1 alpha,25-dihydroxy-19-norvitamin D-3) and its 2-epimeric analog (14-epi-MART-11) were efficiently synthesized using the Julia coupling reaction to connect between the C5 and C6 positions (steroid numbering). An A-ring precursor was prepared from (-)-quinic acid as shown in the previous MART-10 synthesis. The novel 14-epi-CD-ring coupling partner with an elongated two carbon unit as a sulfone was synthesized from 14-epi-25-hydroxy Grundmann's ketone in good yield. The subsequent coupling reaction followed by a deprotection step afforded a mixture of 14-epi-MART-10 and 14-epi-MART-11 in 40% yield. To separate 14-epi-MART-10 and 14-epi-MART-11, each primary hydroxyl group was esterified with a pivaloyl group and the resulting pivalates 2 alpha and 2 beta were separated by high performance liquid chromatography. After the separation, the C2-stereochemistry of each (2 alpha or 2 beta) was determined by H-1 NMR (nuclear magnetic resonance) studies including NOE (nuclear Overhauser effect) experiments. The pivaloyl group was removed under basic conditions to obtain the target molecules of 14-epi-MART-10 and 14-epi-MART-11, respectively. The VDR (vitamin D receptor)-binding affinity, HL-60 (human promyelocytic leukemia) cell differentiation activity, antiproliferative activity in PZ-HPV-7 (immortalized normal prostate) cells and transactivation activity of the osteocalcin promoter in HOS (human osteoblast cell line) cells (serum-free conditions) were investigated. In addition, the effects on bone mineral density (BMD) and the blood and urine calcium concentrations of ovariectomized (OVX) rats were examined. 14-epi-MART-10 has much greater antiproliferative and cell differentiation activities compared to 1 alpha,25-dihydroxyvitamin D-3 (1 alpha,25(OH)(2)D-3).

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PHARMACEUTICAL SOC JAPAN  

We synthesized the 4-hydroxy and 4-methoxy analogs of active vitamin D, (1 alpha,25(OH)(2)D-3, 1) and its C-14-epimer with the previtamin D-3 form of 14-epi-1 alpha,25(OH)(2)preD(3) (14-epi-pre1). Their vitamin D receptor (VDR) binding affinity and osteocalcin promoter transactivation activity in HOS cells were evaluated, and had lower activity than the natural hormone (1) and 14-epi-pre1, respectively.

DOI： 10.1248/cpb.57.1431

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

We describe a novel synthesis of various carbamate- and urea-linked disaccharides stereospecifically using sugar carboxylic acids and sugar alcohols or sugar amines by the modified Curtius rearrangement. In this reaction, the reactivity of each hydroxyl group in glucose as an acceptor has been disclosed. Furthermore. we applied this method to the synthesis of carbamate-linked oligosaccharides including a dendritic molecule. (C) 2008 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tet.2008.06.089

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-V C H VERLAG GMBH  

We describe a novel stereospecific synthesis of various carbamate-linked disaccharides using sugar carboxylic acids and sugar alcohols by a modified Curtius rearrangement. Furthermore, we applied this method to the synthesis of carbamate-linked oligosaccharides including a dendritic molecule. (C) Wiley-VCH Verlag GmbH & Co.

DOI： 10.1002/ejoc.200600993

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

We developed a synthetic method for 5-thio-l-hexopyranose from d-glyconothio-O-lactone using the initial ring-opening reaction and subsequent recyclization under Mitsunobu conditions. Using this method, only a three-step transformation is required from d-glyconothio-O-lactone to 5-thio-l-glyconolactone. © 2005 The Japan Institute of Heterocyclic Chemistry.

DOI： 10.3987/COM-05-S(K)52

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier Ltd  

Azaoligosaccharides are prepared through the glycosylation of nojirimycin derivatives with catalytic amounts of TMSOTf. Also, the 1-6′, 1′-6-cyclic azadisaccharides are successfully synthesized, and their one-pot syntheses are demonstrated. © 2005 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tetlet.2005.02.057

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier Ltd  

1-Deoxy-azasugars are efficiently prepared from sugar-lactones using a stereoinversion process and they are applied to the synthesis of a natural product. © 2003 Elsevier Science Ltd. All rights reserved.

DOI： 10.1016/S0040-4039(03)00512-4

Scopus

researchmap

Publishing type：Research paper (scientific journal)  

A new formacetal linkage (ROCH2OR′) forming reaction was developed, which exploited a combination of sulfide (R′OCH2SR″) and CuBr2-Bu4NBr. This reaction proceeded to give high yields under neutral conditions and was applied to the protection of alcohols, phenols and carboxylic acids by several types of α-oxymethyl groups. © 2001 Elsevier Science Ltd.

DOI： 10.1016/S0040-4039(01)00212-X

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

An enantioselective total synthesis of epothilones A (1) and B (2) using multifunctional asymmetric catalysis such as a cyanosilylation of an aldehyde, an aldol reaction of an unmodified ketone with an aldehyde, and a protonation in the conjugate addition of a thiol to an alpha,beta -unsaturated thioester has been achieved. We divided 1 and 2 into fragment A, fragment B, and fragment C. A catalytic asymmetric synthesis of fragments A and B was accomplished using a catalytic asymmetric cyanosilylation as a key step. An enantiocontrolled synthesis of fragment C was achieved in two ways. One is the use of a direct catalytic asymmetric aldol reaction of an unmodified ketone with an aldehyde as a key step, and the other utilizes a catalytic asymmetric protonation in the conjugate addition of a thiol to an alpha,beta -unsaturated thioester as a key step. Suzuki cross-coupling of fragment A with fragment C followed by Yamaguchi lactonization as key steps led to an enantiocontrolled synthesis of epothilone A (1). On the other hand, Suzuki cross-coupling of fragment B with fragment C followed by Yamaguchi lactonization accomplished an enantiocontrolled synthesis of epothilone B (2).

DOI： 10.1021/ja002024b

Web of Science

Scopus

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-V C H VERLAG GMBH  

A catalytic version has now been developed for the enantioselective total synthesis of epothilone A (1). The key is the use of multifunctional asymmetric catalySes for a direct aldol reaction and cyanosilylation. This successful approach demonstrated the usefulness of these reactions for the catalytic asymmetric synthesis of complex molecules.

DOI： 10.1002/(SICI)1521-3773(20000103)39:1<209::AID-ANIE209>3.0.CO;2-F

Web of Science

Scopus

researchmap

Language：English   Publisher：AMER CHEMICAL SOC  

DOI： 10.1021/ja983895c

Web of Science

Scopus

researchmap

Publishing type：Research paper (scientific journal)  

13-Deacetoxy-11-demethyl-phorbol derivatives with acyl groups of various lengths (from hexanoyl to tetradecanoyl) at the C-12 position were synthesized in an optically active form. Although considerable binding affinities to PKC were observed by analogs 3-7, no activation of PKC was seen even at 10 μM.

DOI： 10.1248/cpb.44.463

Scopus

PubMed

researchmap

Language：Japanese   Publisher：Symposium on the chemistry of natural products  

The enzyme protein kinase C (PKC) is thought to play a major role in cellular signal transduction and has been shown to exist as several isoforms. PKC is activated in cells by diacylglycerol and other diverse tumor promotors such as phorbol 12-myristate-13-acetate (1; PMA). Computer modeling studies on PMA have suggested that carbonyl group at C-3, hydroxyl groups at C-4, C-9, and C-20, and a long-chain hydrophobic portion at C-12 would be important to bind with PKC. However these structural hypotheses have not been confirmed by determining the actual PKC binding affinity or activity relationships of such substituted phorbols. We report here the entire stereospecific syntheses of phorbol analogs with the proposed necessary structural features in PKC activation. In particular, these include the syntheses of the analogs 7 and 10 having long-chain hydrophobic moieties at C-12 of their phorbol C-rings. The key intermediate 18 for the synthesis of these analogs is prepared from (+)-3-carene in a stereocontrolled manner. For the synthesis of 10, treatment of 18 with the phosphonate carbanion generated from 19 resulted in stereospecific formation of the E-stereoisomer of 20. DIBAH reduction of 20 and protection of the resulting primary alcohol, as its tert-butyldiphenylsilyl ether, yielded 21. A three-step series of reactions involving tosylation, iodide substitution, and nitration gave 22. The next crucial step was intramolecular cyclization of 22 to the corresponding isoxazolin 23, which was subsequently hydrogenated to the hydroxy ketone 24 in 85% yield (2 steps). Internal cerium alkoxide assisted propynylation of 24 was then followed by oxidation and conversion of the resulting intermediate into the ethyl-ketone 26. Intramolecular aldol cyclization of 26 and dehydration of the resulting aldol mixture in one-pot, followed by deprotection of the silyl ethers, gave the analog 9. Its allylic alcohol functionality was protected as its trityl ether, and the long-chain myristate group was then introduced at C-12. Selective deprotection of the trityl group of the resulting intermediate afforded the phorbol analog 10. The remaining hydrophobic analog 7 was obtained from 18 using similar methods to those used in the conversion of 18 to 10. The in vitro binding affinity of the phorbol analogs 7 and 10, relative to that of PMA (1), was then evaluated. The analog 7 proved to be very weak with respect to its affinity to bind to PKC, and an approximate 100 fold concentration of 10 was required. These results suggested that either the additional methyl and/or the acetoxyl groups at C-11 and C-13 respectively of 1 are also responsible for its high binding affinity to PKC. They may also be attributed to the presence of too much hydrophobic character due to the long chain at C-12 of the deacetoxy analog 10 resulting in a different hydrophobic interaction with phosphatidyl serine or PKC. In fact, an analog which has octanoyl group at C-12 shows higher binding affinity than that of 10.

DOI： 10.24496/tennenyuki.37.0_19

CiNii Article

CiNii Books

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

Publishing type：Book review, literature introduction, etc.  

Aminobenzopyranoxanthene (ABPX) dye, developed by our group, exhibits two-step color change in solution through a spiro-ring opening/closing process in response to chemical stimuli such as acids, metal ions and phenols. Using this unique characteristic, we have developed ABPX-based colorimetric sensor that change color depending on Cu2+ concentration, enabling visualization by spectrophotometry and the naked eye. Furthermore, an efficient and practical method to prepare ABPX with various nitrogen-containing fused rings was developed. Using a simple acid-catalyzed reaction consisting of 1,3-diisopropoxybenzene and CH 3SO 3H, a variety of benzophenones gave ABPX directly in excellent yields. We discovered that ABPX is a new type of mechanochromic organic molecule with a large wavelength difference of fluorescence (near-IR/blue). Detailed spectrophotometric and single-crystal X-ray analyses revealed that the near-IR fluorescence is attributable to fluorescence from slip-stacked dimeric structures in crystals, while the blue fluorescence is attributable to fluorescence from the monomer. Switching between the two is achieved by dynamic structural interconversion in response to mechanical grinding and exposure to the vapor of solvents.

DOI： 10.5059/yukigoseikyokaishi.76.1066

Scopus

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：Japanese  

J-GLOBAL

researchmap

Language：Japanese   Publisher：公益社団法人 日本ビタミン学会  

DOI： 10.20632/vso.87.11_652

CiNii Article

researchmap

Language：Japanese   Publisher：公益社団法人 日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

DOI： 10.20632/vso.86.12_694

CiNii Article

CiNii Books

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

DOI： 10.20632/vso.86.3_181

CiNii Article

researchmap

Language：English   Publishing type：Research paper, summary (international conference)   Publisher：INT INST ANTICANCER RESEARCH  

Web of Science

researchmap

Language：English   Publishing type：Research paper, summary (international conference)   Publisher：INT INST ANTICANCER RESEARCH  

Web of Science

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

DOI： 10.20632/vso.85.3_166_2

CiNii Article

researchmap

Language：Japanese   Publisher：公益社団法人 日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：Japanese   Publisher：公益社団法人 日本ビタミン学会  

CiNii Article

researchmap

Language：Japanese   Publisher：公益社団法人 日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：Japanese   Publisher：公益社団法人 日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

DOI： 10.20632/vso.83.10_593_1

CiNii Article

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

DOI： 10.20632/vso.83.5-6_328

CiNii Article

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：English   Publishing type：Research paper, summary (international conference)   Publisher：AMER CHEMICAL SOC  

Web of Science

researchmap

Language：English   Publishing type：Research paper, summary (international conference)   Publisher：AMER CHEMICAL SOC  

Web of Science

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：English   Publishing type：Research paper, summary (international conference)   Publisher：INT INST ANTICANCER RESEARCH  

Web of Science

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

DOI： 10.20632/vso.81.11_582

CiNii Article

researchmap

Language：Japanese   Publisher：日本ビタミン学会  

CiNii Article

CiNii Books

researchmap

Language：English   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

We describe a facile synthetic method of carbamate- and urea-linked glycoconjugates using sugar carboxylic acids by the modified Curtius rearrangement. This reaction is a simple one-pot procedure, and various nucleophiles including tertiary alcohols can be utilized to afford desired compounds in moderate to high yields. And the stereospecific synthesis of the anomeric isomers is achieved using the corresponding two stereoisomers of glycosyl carboxylic acid. (c) 2006 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tetlet.2006.07.139

Web of Science

Scopus

CiNii Article

researchmap

Language：English   Publisher：WILEY-V C H VERLAG GMBH  

An efficient divergent synthesis Of L-sugars and L-iminosugars from D-sugars is described. The important intermediate, delta-hydroxyalkoxamate, prepared from D-glucono-/galactono-1,5-lactone, was cyclized under Mitsunobu conditions to give the O-cyclized oxime compound and the N-cyclized lactam compound as mixtures. A more detailed investigation revealed that the appropriate protecting groups and solvents controlled the specificity for the O-/N-cyclization of the delta-hydroxyalkoxamate. Suitable protection at the delta-position of 6-hydroxyalkoxamate, derived from D-glucono-1,5-lactone, afforded the corresponding delta-alkylation product alone. Thus we succeeded in applying this to the total synthesis of L-iduronic acid. In contrast, with both TBDMS as the protecting group and RCN as the solvent the efficient conversion of D-glucono/galactono-1,5-lactone into the corresponding L-iminosugars (L-idonolactam and L-altronolactam) was achieved.

DOI： 10.1002/chem.200600268

Web of Science

Scopus

PubMed

CiNii Article

researchmap

A new bifunctional asymmetric catalyst containing a Lewis acid and a Lewis base (1) was developed and applied to the catalytic asymmetric cyanosilylation of aldehydes. The products were obtained generally with excellent enantiomeric excess. The experiments using the control catalyst (5) and the catalyst containing more electron-rich phosphine oxide (6) suggest that the catalyst 1 should promote the reaction via a dual activation of the aldehyde by the aluminum and TMSCN by the phosphine oxide. This reaction is practical and was applied to the catalytic asymmetric total synthesis of epothilone A. © 2001 Elsevier Science Ltd.

DOI： 10.1016/S0040-4020(00)01039-5

Scopus

researchmap