Faculty Profiles - MORIHARA Ryuuta

Papers - MORIHARA Ryuuta

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The potential mechanism maintaining transactive response DNA binding protein 43 kDa in the mouse stroke model. International journal

Yuting Bian, Yusuke Fukui, Ricardo Satoshi Ota-Elliott, Xinran Hu, Hongming Sun, Zhihong Bian, Yun Zhai, Haibo Yu, Xiao Hu, Hangping An, Hongzhi Liu, Ryuta Morihara, Hiroyuki Ishiura, Toru Yamashita

Neuroscience research 2025.1

Language：English Publishing type：Research paper (scientific journal)

The disruption of transactive response DNA binding protein 43 kDa (TDP-43) shuttling leads to the depletion of nuclear localization and the cytoplasmic accumulation of TDP-43. We aimed to evaluate the mechanism underlying the behavior of TDP-43 in ischemic stroke. Adult male C57BL/6 J mice were subjected to 30 or 60 min of transient middle cerebral artery occlusion (tMCAO), and examined at 1, 6, and 24 h post reperfusion. Immunostaining was used to evaluate the expression of TDP-43, G3BP1, HDAC6, and RAD23B. The total and cytoplasmic number of TDP-43-positive cells increased compared with sham operation group and peaked at 6 h post reperfusion after tMCAO. The elevated expression of G3BP1 protein peaked at 6 h after reperfusion and decreased at 24 h after reperfusion in ischemic mice brains. We also observed an increase of expression level of HDAC6 and the number of RAD23B-positive cells increased after tMCAO. RAD23B was colocalized with TDP-43 24 h after tMCAO. We proposed that the formation of stress granules might be involved in the mislocalization of TDP-43, based on an evaluation of G3BP1 and HDAC6. Subsequently, RAD23B, may also contribute to the downstream degradation of mislocalized TDP-43 in mice tMCAO model.

DOI： 10.1016/j.neures.2025.01.006

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レカネマブ治療に伴うAPOE遺伝子型検査の実際

森原隆太, 小坂田陽介, 池上憲, 平祐貴, 石田将大, 樹下明典, 柚木太淳, 武本麻美, 野村恵美, 河野智仁, 松岡千加, 山下徹, 加藤芙美乃, 深野智華, 平沢晃, 石浦浩之

Dementia Japan 38 ( 4 ) 681 - 681 2024.10

髄液中抗MOG抗体を指標にIVIGによる維持療法を行った再発性MOGADの一例

池上憲, 佐々木諒, 石田将大, 樹下明典, 平祐貴, 小坂田陽介, 武本麻美, 森原隆太, 柚木太淳, 山下徹, 石浦浩之

神経免疫学 29 ( 1 ) 262 - 262 2024.10

ATXN2遺伝子のCAGリピートをホモ接合性に有し同胞と臨床病型が異なりパーキンソニズムと痙性を呈した症例

小坂田陽介, 松岡千加, 中田有美, 佐々木諒, 中野由美子, 柚木太淳, 武本麻美, 森原隆太, 山下徹, 石浦浩之

臨床神経学 64 ( 10 ) 755 - 755 2024.10

当院における2家系のSCAR8の検討

柚木太淳, 松岡千加, 小坂田陽介, 中野由美子, 武本麻美, 森原隆太, 山下徹, 石浦浩之

臨床神経学 64 ( 10 ) 751 - 751 2024.10

Suppression of PTBP1 in hippocampal astrocytes promotes neurogenesis and ameliorates recognition memory in mice with cerebral ischemia. Reviewed International journal

Yusuke Fukui, Ryuta Morihara, Xinran Hu, Yumiko Nakano, Taijun Yunoki, Mami Takemoto, Koji Abe, Toru Yamashita

Scientific reports 14 ( 1 ) 20521 - 20521 2024.9

Language：English Publishing type：Research paper (scientific journal)

The therapeutic potential of suppressing polypyrimidine tract-binding protein 1 (Ptbp1) messenger RNA by viral transduction in a post-stroke dementia mouse model has not yet been examined. In this study, 3 days after cerebral ischemia, we injected a viral vector cocktail containing adeno-associated virus (AAV)-pGFAP-mCherry and AAV-pGFAP-CasRx (control vector) or a cocktail of AAV-pGFAP-mCherry and AAV-pGFAP-CasRx-SgRNA-(Ptbp1) (1:5, 1.0 × 1011 viral genomes) into post-stroke mice via the tail vein. We observed new mCherry/NeuN double-positive neuron-like cells in the hippocampus 56 days after cerebral ischemia. A portion of mCherry/GFAP double-positive astrocyte-like glia could have been converted into new mCherry/NeuN double-positive neuron-like cells with morphological changes. The new neuronal cells integrated into the dentate gyrus and recognition memory was significantly ameliorated. These results demonstrated that the in vivo conversion of hippocampal astrocyte-like glia into functional new neurons by the suppression of Ptbp1 might be a therapeutic strategy for post-stroke dementia.

DOI： 10.1038/s41598-024-71212-w

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Protective effect of scallop-derived plasmalogen against vascular dysfunction, via the pSTAT3/PIM1/NFATc1 axis, in a novel mouse model of Alzheimer's disease with cerebral hypoperfusion. Reviewed International journal

Yun Zhai, Ryuta Morihara, Tian Feng, Xinran Hu, Yusuke Fukui, Zhihong Bian, Yuting Bian, Haibo Yu, Hongming Sun, Mami Takemoto, Yumiko Nakano, Taijun Yunoki, Ying Tang, Hiroyuki Ishiura, Toru Yamashita

Brain research 1828 148790 - 148790 2024.4

Language：English Publishing type：Research paper (scientific journal)

A strong relationship between Alzheimer's disease (AD) and vascular dysfunction has been the focus of increasing attention in aging societies. In the present study, we examined the long-term effect of scallop-derived plasmalogen (sPlas) on vascular remodeling-related proteins in the brain of an AD with cerebral hypoperfusion (HP) mouse model. We demonstrated, for the first time, that cerebral HP activated the axis of the receptor for advanced glycation endproducts (RAGE)/phosphorylated signal transducer and activator of transcription 3 (pSTAT3)/provirus integration site for Moloney murine leukemia virus 1 (PIM1)/nuclear factor of activated T cells 1 (NFATc1), accounting for such cerebral vascular remodeling. Moreover, we also found that cerebral HP accelerated pSTAT3-mediated astrogliosis and activation of the nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) inflammasome, probably leading to cognitive decline. On the other hand, sPlas treatment attenuated the activation of the pSTAT3/PIM1/NFATc1 axis independent of RAGE and significantly suppressed NLRP3 inflammasome activation, demonstrating the beneficial effect on AD.

DOI： 10.1016/j.brainres.2024.148790

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【指定難病 Up to Date】前頭側頭葉変性症

森原隆太, 石浦浩之

岡山医学会雑誌 136 ( 1 ) 17 - 21 2024.4

リウマチ性疾患の中枢神経症状と神経自己抗体

高橋幸利, 井口晃宏, 矢部友奈, 千葉悠平, 伊倉崇浩, 須田顕, 山口佳剛, 土屋遙香, 土田優美, 中馬越清隆, 寺田真, 横手顕, 武井藍, 森原隆太

リウマチ科 71 ( 4 ) 444 - 452 2024.4

Neuroprotective effect of, a flavonoid, sudachitin in mice stroke model. Reviewed International journal

Ricardo Satoshi Ota-Elliott, Yusuke Fukui, Yuting Bian, Zhihong Bian, Xinran Hu, Hongming Sun, Haibo Yu, Ryuta Morihara, Hiroyuki Ishiura, Toru Yamashita

Brain research 1827 148745 - 148745 2024.3

Language：English Publishing type：Research paper (scientific journal)

A flavonoid, sudachitin, has been reported to show some beneficial health effects, including as an anti-inflammatory in LPS-stimulated macrophages, as well as improving glucose and lipid metabolism in mice fed a high-fat diet. In this study, we investigated the neuroprotective effect of sudachitin in the transient middle cerebral artery occlusion (tMCAO) mouse model. After daily pre-treatment of vehicle or sudachitin (5 or 50 mg/kg) for 14 days, mice (n = 76) were subjected to a sham operation or tMCAO for 45 min, and on the following days, they were treated daily with vehicle or sudachitin. The administration of sudachitin significantly reduced (p < 0.05) cerebral infarct volume and attenuated apoptosis, 5 days after tMCAO. Neurological impairment improved, the expression of an oxidative stress marker, 4-HNE, decreased, and the Sirt1/PGC-1α pathway was activated 5 days after tMCAO in the sudachitin-treated group. This is the first report to demonstrate the neuroprotective effect of sudachitin in cerebral ischemia/reperfusion injury mice model, probably by activating the Sirt1/PGC-1α axis. Sudachitin may be a promising supplement or therapeutic agent for reducing injury caused by ischemic strokes.

DOI： 10.1016/j.brainres.2023.148745

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脳血栓回収療法後の認知機能の転帰の検討 Reviewed

森原隆太, 小坂田陽介, 角田慶一郎, 久我純弘, 阿部康二, 大西英之, 石浦浩之, 山下徹

日本脳血管・認知症学会総会プログラム・抄録集 14th 2024

A Novel Peptidome Technology for the Diagnosis of Mild Cognitive Impairment and Alzheimer's Disease by Selected Reaction Monitoring. Reviewed International journal

Yusuke Fukui, Koh Tadokoro, Minaki Hamada, Kyoichi Asada, Lyang-Ja Lee, Hidehisa Tachiki, Ryuta Morihara, Koji Abe, Toru Yamashita

Journal of Alzheimer's disease : JAD 100 ( 1 ) 219 - 228 2024

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: With the aging of populations worldwide, Alzheimer's disease (AD) has become a concern due to its high prevalence and the continued lack of established treatments. Early diagnosis is required as a preventive intervention to modify the disease's progression. In our previous study, we performed peptidomic analysis of serum samples obtained from AD patients and age-matched healthy subjects to seek peptide biomarker candidates for AD by using BLOTCHIP-MS analysis, and identified four peptides as AD biomarker candidates. OBJECTIVE: The objective was to validate the serum biomarker peptides to distinguish mild cognitive impairment (MCI) and AD in comparison to cognitively healthy controls using a new peptidome technology, the Dementia Risk Test. METHODS: We enrolled 195 subjects with normal cognitive function (NC; n = 70), MCI (n = 55), and AD (n = 70), The concentrations of cognitive impairment marker peptides (Fibrinogen α chain (FAC), Fibrinogen β chain (FBC), Plasma protease C1 inhibitor (PPC1I), α2-HS-glycoprotein (AHSG)) were quantified by using a selected reaction monitoring assay based on liquid chromatography-MS/MS. RESULTS: The present study confirmed that three peptides, FAC, FBC, and PPC1I, were significantly upregulated during the onset of AD. This three-peptide set was both highly sensitive in determining AD (sensitivity: 85.7%, specificity: 95.7%, AUC: 0.900) and useful in distinguishing MCI (sensitivity: 61.8%, specificity: 98.6%, AUC: 0.824) from NC. CONCLUSIONS: In this validation study, we confirmed the high diagnostic potential of the three peptides identified in our previous study as candidate serum biomarkers for AD. The Dementia Risk Test may be a powerful tool for detecting AD-related pathological changes.

DOI： 10.3233/JAD-230915

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A Unique Case of Sarcoid-associated Myelopathy Accompanied by Lung Cancer. Reviewed

Koh Tadokoro, Yumi Nakada, Ryo Sasaki, Yumiko Nakano, Taijun Yunoki, Kotaro Shin, Masataka Taoka, Kiichiro Ninomiya, Emi Nomura, Mami Takemoto, Ryuta Morihara, Toru Yamashita

Internal medicine (Tokyo, Japan) 62 ( 23 ) 3531 - 3535 2023.12

Language：English Publishing type：Research paper (scientific journal)

The differential diagnosis of myelopathy in patients with malignancies may be challenging, as a spinal biopsy is not always applicable. A 66-year-old woman who had shown transient double vision and nausea developed spasticity and impaired deep sensation in both feet. Magnetic resonance imaging showed abnormal gadolinium enhancement of the brainstem, spinal meninges, and nerve root. Cerebrospinal fluid (CSF) revealed mild pleocytosis and elevated protein and decreased glucose levels, although CSF cytology was normal. Lung carcinoma was simultaneously detected, and noncaseating granuloma was detected from the hilar and axillary lymph nodes, so she was diagnosed with sarcoid-associated myelopathy. Her symptoms were kept stable by intravenous methylprednisolone, oral prednisolone, and methotrexate. This is the first case of sarcoid-associated myelopathy accompanied by lung cancer, suggesting the importance of clinical course, repetitive CSF cytology, and a biopsy of the lymph nodes to distinguish sarcoid-associated myelopathy from meningeal metastasis in patients with malignancies.

DOI： 10.2169/internalmedicine.0943-22

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Exogenous amyloid-β oligomers exacerbate cognitive deficits and activated necroptosis in APP23 mice(タイトル和訳中) Reviewed

胡欣冉, 于海波, 山下徹, 森原隆太, 福井裕介, 石浦浩之

脳循環代謝 35 ( 1 ) 122 - 122 2023.11

外因性アミロイドβオリゴマーはAPP23マウスにおける認知障害を悪化させネクロトーシスを活性化させる(Exogenous amyloid-β oligomers exacerbate cognitive deficits and activated necroptosis in APP23 mice) Reviewed

胡欣冉, 于海波, 山下徹, 森原隆太, 福井裕介, 石浦浩之

脳循環代謝 35 ( 1 ) 122 - 122 2023.11

脳血栓回収療法がpost-stroke dementiaをひきおこすのか?

小坂田陽介, 森原隆太, 久我純弘, 阿部康二, 大西英之, 山下徹

神経科学研究所医誌 8 ( 1 ) 43 - 47 2023.11

Language：Japanese Publisher：(医社)英明会大西脳神経外科病院神経科学研究所

50歳以上のテント上急性期脳梗塞症例454例のうち31例を対象に、脳血栓回収療法がpost-stroke dementiaに与える影響について検討した。対象は内科治療群20例と血管内治療群11例に分け、脳梗塞発症後12ヵ月まで経過観察を行い認知機能を比較した。その結果、発症12ヵ月後の認知機能は、内科治療群でMMSEおよびMoCAで有意な改善を認めたが、血管内治療群は認知機能は改善せず、特にMoCAで差があった。以上より、脳血栓回収療法による血管壁への機械的侵襲や虚血再灌流障害はpost stroke dementiaのリスク因子となる可能性がある。

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Protective Effects of Rivaroxaban on White Matter Integrity and Remyelination in a Mouse Model of Alzheimer's Disease Combined with Cerebral Hypoperfusion. Reviewed International journal

Zhihong Bian, Xinran Hu, Xia Liu, Haibo Yu, Yuting Bian, Hongming Sun, Yusuke Fukui, Ryuta Morihara, Hiroyuki Ishiura, Toru Yamashita

Journal of Alzheimer's disease : JAD 96 ( 2 ) 609 - 622 2023.10

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: Alzheimer's disease (AD) is characterized by cognitive dysfunction and memory loss that is accompanied by pathological changes to white matter. Some clinical and animal research revealed that AD combined with chronic cerebral hypoperfusion (CCH) exacerbates AD progression by inducing blood-brain barrier dysfunction and fibrinogen deposition. Rivaroxaban, an anticoagulant, has been shown to reduce the rates of dementia in atrial fibrillation patients, but its effects on white matter and the underlying mechanisms are unclear. OBJECTIVE: The main purpose of this study was to explore the therapeutic effect of rivaroxaban on the white matter of AD+CCH mice. METHODS: In this study, the therapeutic effects of rivaroxaban on white matter in a mouse AD+CCH model were investigated to explore the potential mechanisms involving fibrinogen deposition, inflammation, and oxidative stress on remyelination in white matter. RESULTS: The results indicate that rivaroxaban significantly attenuated fibrinogen deposition, fibrinogen-related microglia activation, oxidative stress, and enhanced demyelination in AD+CCH mice, leading to improved white matter integrity, reduced axonal damage, and restored myelin loss. CONCLUSIONS: These findings suggest that long-term administration of rivaroxaban might reduce the risk of dementia.

DOI： 10.3233/JAD-230413

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視線計測計を用いた認知機能検査の有用性 Reviewed

田所功, 福井裕介, 野村恵美, 中野由美子, 柚木太淳, 武本麻美, 森原隆太, 阿部康二, 山下徹

日本老年医学会雑誌 60 ( 4 ) 476 - 477 2023.10

Injection of exogenous amyloid-β oligomers aggravated cognitive deficits, and activated necroptosis, in APP23 transgenic mice. International journal

Haibo Yu, Ryuta Morihara, Ricardo Ota-Elliott, Zhihong Bian, Yuting Bian, Xinran Hu, Hongming Sun, Yusuke Fukui, Koji Abe, Hiroyuki Ishiura, Toru Yamashita

Brain research 1821 148565 - 148565 2023.9

Language：English Publishing type：Research paper (scientific journal)

Alzheimer's disease (AD) is a neurodegenerative disease that is characterized by the loss of synapses and neurons in the brain, and the accumulation of amyloid plaques. Aβ oligomers (AβO) play a critical role in the pathogenesis of AD. Although there is increasing evidence to support the involvement of necroptosis in the pathogenesis of AD, the exact mechanism remains elusive. In the present study, we explored the effect of exogenous AβO injection on cell necroptosis and cognitive deficits in APP23 transgenic mice. We found that intrahippocampal injection of AβO accelerated the development of AD pathology and caused cognitive impairment in APP23 mice. Specifically, AβO injection significantly accelerated the accumulation of AβO and increased the expression level of phosphorylated-tau, and also induced necroptosis. Behavioral tests showed that AβO injection was associated with cognitive impairment. Furthermore, necroptosis induced by AβO injection occurred predominantly in microglia of the AD brain. We speculate that AβO increased necroptosis by activating microglia, resulting in cognitive deficits. Our results may aid in an understanding of the role played by AβO in AD from an alternative perspective and provide new ideas and evidence for necroptosis as a potential intervention and therapeutic target for AD.

DOI： 10.1016/j.brainres.2023.148565

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抗AQP4抗体陽性のNMOsd患者の髄液におけるIL-6測定の有用性の検討

柚木太淳, 中田有美, 松岡千加, 田所功, 佐々木諒, 中野由美子, 武本麻実, 森原隆太, 山下徹, 石浦浩之

臨床神経学 63 ( Suppl. ) S298 - S298 2023.9

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Papers - MORIHARA Ryuuta